LOCUS       DASTWE010000001         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_387871, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000001 DASTWE010000000
VERSION     DASTWE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387871"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..841)
                     /locus_tag="VFT30_00005"
     CDS             complement(47..841)
                     /locus_tag="VFT30_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEU5091033.1"
                     /translation="MRWIILSLIIVTTGCSTVPAMFTPAEPIPLNEFSHRLLSRILGA
                     HVSNGVVDYPGIQVDDRLSAYLAQLDRVDPNAFVTRNERLAFWINAYNAFAIKGILDH
                     YSPVSYVGRYRYFIGRDYRVGGAGINLYDLERQVLMKQFQEPRIHFAIVCASMSCPKL
                     QPWVYEPDQLERQLDDVARDFINDPTRNRFDRTQKVASLSMIFKWFDDDFTRAAGSVL
                     TYITRYVNDPELVHDLTQSEYRIEYLDYDWSLNGIPPKEPSHAGRS"
     gene            complement(858..1916)
                     /locus_tag="VFT30_00010"
     CDS             complement(858..1916)
                     /locus_tag="VFT30_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5091034.1"
                     /translation="MLIHIERSQYRPSGQIEGRSTGKSERTSLYRALWGGLTNLVLRR
                     PLLAVFQVNLRCNSSCGYCNLPLNVGRYEMSQQEIRDVFAGLYRGGLRFVFVQGGEPL
                     LRRDLPDILQDLVEIGFHLTLITNGIKLTPHLVQQFDDLSVSLSISLDTLDRNKYERI
                     RGADQLEAVLTGLDLLQHYRCPKFLICIVSEINRDEVKNVVAFARQRGFLPVVGAYHW
                     DVGLYGKQDATLMYDRQQARLVFERLLEQDILPPGYLRQYAKDNVAWLRGETLKPCDA
                     GRYSIAIDASGNVAPCLSLPAVGNLLESSLSEILARLDRQEIQHCSDRSSCNRVDGRV
                     IGSVLRHPIAAWQTPVTW"
BASE COUNT          553 a          609 c          549 g          535 t
ORIGIN      
        1 accatgttcc aaaaacgtca ggagcctggc gatgggcacc ctctcatcat gaccgaccag
       61 catgagaagg ctcctttggt gggatcccgt tcaggctcca gtcgtagtct agatattcga
      121 tacggtattc cgattgcgtc aagtcgtgaa ccagttccgg atcgttcaca tagcgggtga
      181 tgtaggttaa cactgaccca gcagccctcg tgaaatcgtc gtcaaaccac ttgaagatca
      241 tcgaaagtga cgcaactttc tgcgtgcggt cgaaacgatt gcgcgtggga tcgttaatga
      301 agtccctggc gacatcatcg agctggcgtt cgagctgatc cggttcatat acccatggct
      361 ggagctttgg gcaggacatg gaggcacaca cgatcgcgaa atgtatccgc ggttcctgaa
      421 attgcttcat cagcacctgc cgttccagat cgtacagatt gatgccggct cccccaaccc
      481 gatagtcccg cccaatgaaa tagcgataac gtcccacata gctcaccggc gagtagtgat
      541 cgagaatgcc cttgatcgca aacgcattgt acgcgttgat ccagaaggcc agccgttcat
      601 tgcgcgtgac aaacgcgttg gggtcaactc ggtctagttg ggcgaggtaa gccgacaatc
      661 gatcatccac ctggatgcct ggataatcga caactccatt gctcacatgt gcccccaata
      721 ttcgactcaa cagtctgtgc gaaaattcat tgagtggaat aggttcggct ggtgtgaaca
      781 ttgccggaac agttgagcaa ccagtggtca caatgatcag gctcagtatg atccatcgca
      841 tcgtaacctc tcttgaccta ccacgtaacc ggtgtttgcc acgctgcgat cggatgtcgc
      901 agtaccgacc cgatgacccg tccgtctact ctgttgcagg acgatcgatc tgaacaatgc
      961 tgaatctctt gtcgatcgag ccgggcgagt atttcactga gtgaagactc aagaagattt
     1021 ccgaccgcag gaagcgacaa gcacggcgca acatttccgg acgcgtcgat tgcgatgctg
     1081 tatctacccg catcgcacgg ctttaatgtt tctccacgaa gccatgcaac attgtccttc
     1141 gcatattgcc gaaggtagcc aggaggcagg atatcctgtt caagcaatcg ctcgaagacc
     1201 aaccgggctt gttgacggtc atacatcaac gtcgcatcct gcttgccata caaccccaca
     1261 tcccaatggt aggcgccgac aaccggcaag aaaccccgtt gcctggcgaa tgcaacaaca
     1321 ttctttactt catcccgatt aatctcgctc acgatacaga ttagaaattt tggacagcga
     1381 tagtgctgga gaagatcaag gccggtaagg accgcttcaa gttggtcagc accccggata
     1441 cgttcgtact tgttgcgatc caaagtgtcg aggctaatgg agagtgatac cgagagatcg
     1501 tcgaattgtt gcactaggtg tggggtcagc ttgattccgt tcgtaatcaa tgtcagatga
     1561 aatcctatct caactaaatc ttgaagaata tcaggtaagt cacggcgtag aagcggctcg
     1621 cctccttgca caaacacaaa acgtaacccg cccctataga gcccagcgaa gacgtcccga
     1681 atctcttgct gtgacatctc ataccgaccg acattcaatg gaagattgca gtaaccacag
     1741 gaagaattac aacgaaggtt cacttgaaat acggccaaca gcggacgtct tagaaccaaa
     1801 ttcgtcaacc cgccccacag cgctcggtag agactcgtcc gttcactctt tccagtagag
     1861 cgtccctcaa tctgcccgga tgggcggtac tgtgatcgtt caatatgtat cagcatgacc
     1921 tgaccaggcg acgatcgcgt gcagttttgc tcaacaatgt accgtttttc tccgggtctt
     1981 atggtctttc ctagtagcaa atctctgtaa ctgcgtcgtc gtttcacctt tctctggtcg
     2041 gcacgtactt tgctgcccac tcgtacaatt cattgctcgt accaggaggt gcttaatgat
     2101 gatccatcgc atggaaattc ttgcgggtgt tctgggtact ctagccgctc tttctttgtc
     2161 tataccctcc atgactgccg caagctcaaa agacggagaa aaatcaggtg tctattcgtc
     2221 agtcgcagca gcgacagagg tggctt
//
LOCUS       DASTWE010000002         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1486638, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000002 DASTWE010000000
VERSION     DASTWE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486638"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..>1092)
                     /locus_tag="VFT30_00015"
     CDS             complement(45..>1092)
                     /locus_tag="VFT30_00015"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5091035.1"
                     /translation="PGVNRSGYYNQFNQGKESIFLNLKDPQALEIAKKLVAASDIVSE
                     NFAAGVMDKMGLGYEVMRKIKSDIIMISMSGYGAVGPESPYVSYGPAQAPLSGLSSLT
                     GFADFPPMHVGFSYGDPNGGLHGAFAVLAALMHRARTGEGQYIDLSQWESSIAIIGEG
                     MMAQTMNGVPPPRIGNRDPYMSPHGLFRCRGEDRWVSIVVASEEEWQQFCQGVGQHGL
                     ATDSRFSSLEARKKNEDALEALITTWTEVLTAEEVAQKLQAVGVAAFPAVTNKELAED
                     PHLQNRGFFVELPHPEVGVRRHAGVPWIFSDTPCQVQRPAPCAGQDTDDVLHRVLGYS
                     SDEIAAFRAKGVLT"
BASE COUNT          247 a          312 c          273 g          260 t
ORIGIN      
        1 tgtctgttcg ccttttgcct tttgccttgc attcatgaag tcctctaagt caaaacccct
       61 ttcgcacgga acgcggcgat ctcgtccgac gaatatccca aaacacggtg cagtacatcg
      121 tccgtatctt gtccggcaca cggtgcaggc cgctggactt gacatggcgt atcagaaaat
      181 atccatggta cgccggcgtg ccgacgcacg ccgacttctg gatgcggtaa ttcgacgaag
      241 aatccgcggt tctgtaaatg tggatcttcg gctaactctt tgttggttac agcaggaaat
      301 gcagccacac ctacggcttg taatttttgt gcgacctctt ccgcagtgag tacttcagtc
      361 caagtcgtaa taagcgcttc gagcgcatct tcgttctttt tgcgggcttc tagagaagag
      421 aaacgagagt cagtcgcaag tccatgttgc ccaacacctt gacagaattg ctgccattct
      481 tcttcgctgg ccacgacgat actcacccac cggtcttccc cccggcaacg gaacaatccg
      541 tgtggcgaca tatacggatc gcggttgcca atacgtgggg gtggcacccc attcatcgtc
      601 tgcgccatca taccttcgcc gatgatcgcg atggacgatt cccactgcga gaggtcaatg
      661 tattggcctt caccggtgcg ggcacggtgc ataagtgcgg ccagtacggc aaatgcgcca
      721 tgcaagccgc cgttcgggtc gccataagaa aagccgacat gcatcggcgg gaaatcagca
      781 aatccggtta acgaagacag cccacttaag ggagcttgcg ccggaccgta cgagacatag
      841 gggctttccg gtccgactgc tccatacccg gacatggaaa tcataataat gtccgacttg
      901 attttacgca tgacctcgta gccgaggccc atcttgtcca tcacgccagc agcgaagttc
      961 tcggacacaa tgtcactagc tgcgacaagc ttcttagcga tttccaacgc ttgcggatct
     1021 ttgagattca gaaagatcga ctctttcccc tggttgaatt gattgtaata gccactgcga
     1081 ttcactcccg gc
//
LOCUS       DASTWE010000003         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_969582, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000003 DASTWE010000000
VERSION     DASTWE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969582"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..799)
                     /locus_tag="VFT30_00020"
     CDS             complement(167..799)
                     /locus_tag="VFT30_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5091036.1"
                     /translation="MKSTFTQDSRDSSAVVTSGVADLMTSLAVIFILLLVAYVTRVPD
                     GNANPPGGRATPTDMTPRLDSRHPPLEAKRPSVHTITVPDAAINFEFGKSTLLPTAET
                     FLSEAMPHYASLICKPGEQEVEAFVIEGYTDDLGDDIRNLRLSQERSFAVLAKSLEVI
                     REELPWAYECFLQKATANGRGRQDLLRNDAGHPDRDKSRRVVFKIHMRPA"
     gene            complement(796..2034)
                     /locus_tag="VFT30_00025"
     CDS             complement(796..2034)
                     /locus_tag="VFT30_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091037.1"
                     /translation="MDELDSFTALWKLLTRDVTVVGGLQSPMLSWIGAAGILILCLWH
                     STVFIRGLLQIQRTLSRFQSSVAPLVLARRQVSKEWLVIPALAKRRARLDESPETRRD
                     LDDLQALDRAVRSEQAFARDWLSYRKTLLVEQSAWFLEPTVHCQRSASEFFSFESLCA
                     AHLNVRFYRQLPAFMTGMGLMFTFLAILIGLGKLHANGSEIDGIQGLINGLSGKFVTS
                     IVGLACANAFTLLENSLWHRLDNRHRECISLLDEMFPQKAADQPTQIPSSPNGSPTTM
                     IAPARNDTAHQLVEVVQQRLGSTVAALNSASQALTALNSKHSPMKLDDLPQEIGHEVQ
                     RALRPLVNPLLEAIHDLNRSIKEQCSPVQLSQSDIETMFQGLRSNTAATTEQVRPANK
                     TATQESRVVPHETARKERRG"
BASE COUNT          571 a          622 c          677 g          556 t
ORIGIN      
        1 gagcaattta tcggactgac tcgccgtata tatgtgatga ctctctcgaa cctccaccga
       61 taataactgc ctacctaagg caagtcgcaa acagatgggt ccgatcggag atcgtgctat
      121 gtgaaccagt gatgacggga agccgccgtt gtgcgtgctc ggtacgctat gcgggtcgca
      181 tatggatttt gaaaacgacg cgacggcttt tgtcgcgatc agggtgtccg gcatcattgc
      241 gtaggaggtc ctgtcttccc cgcccgttcg ctgtggcctt ttgcaagaag cactcataag
      301 cccaaggaag ctcctcgcga atcacctcca aacttttcgc cagtactgca aaagagcgtt
      361 cctgactgag cctcagattg cgtatatcgt cccccagatc gtcagtgtac ccttcaatga
      421 caaaggcctc tacttcttgc tctccgggct tgcagatgag cgaggcgtag tgaggcatgg
      481 cctcggacaa gaaggtttca gcagtaggga gcaacgtgct ctttccaaac tcaaagttga
      541 ttgccgcatc cggcaccgtg atcgtgtgga cgctcggcct tttagcctcc agtggtggat
      601 gacgcgagtc aagtctcggc gtcatgtccg tcggtgtagc acgaccaccg ggtggattgg
      661 catttccatc tgggacgcgg gtcacatagg ccacgagcaa tagaatgaag atgacggcga
      721 gcgaggtcat aagatctgca actccactcg tcaccacagc agagctgtcg cgagagtcct
      781 gcgtgaaggt cgacttcatc cccttcgttc cttccgtgcc gtttcatgtg ggacgacacg
      841 gctttcttgg gtcgcggtct tattcgctgg acgtacctgt tccgtcgtcg cagctgtatt
      901 actccggagt ccctgaaaca tggtttcgat atccgattga gacagctgta ccggagagca
      961 ttgctctttg atggagcggt tgagatcgtg aatggcttcc agcaaaggat tcaccagggg
     1021 ccttaacgcc cgttggacct cgtgaccgat ctcttgcggc aaatcatcaa gcttcatcgg
     1081 tgaatgtttg gaattcaaag ccgtgagcgc ctgtgaggcg gagttcagtg ctgcgacggt
     1141 tgatccgaga cgctgctgta cgacctctac aagctgatgc gcggtatcgt ttcgggcggg
     1201 agcgatcatg gtcgttggtg atccgttcgg cgatgaggga atctgagtag gttgatcggc
     1261 agccttctga gggaacattt cgtccagtag ggagatgcac tcccggtgtc gattatccag
     1321 ccgatgccag agagaatttt ctaggagggt gaaggcattg gcacacgcca aaccgacgat
     1381 agatgtgaca aactttcctg agagcccgtt gataagtcct tggattccat cgatttccga
     1441 cccattcgca tggagcttgc cgagaccgat caggatagca aggaaagtga acatcaatcc
     1501 catacccgtc atgaaggcgg gaagctgccg gtaaaagcgg acgttgagat gggcagcgca
     1561 gagagattcg aacgagaaaa actcactggc cgaacgctgg caatgcacgg tcggctcaag
     1621 aaaccaggca gattgctcta ccaagagagt cttgcggtat gaaagccagt ctctggcaaa
     1681 agcctgctcc gaccgtaccg cacgatccag tgcttgcaga tcgtcaagat cgcgtcgcgt
     1741 ttctggcgac tcgtcgagac gcgcccgcct tttcgctaaa gccgggataa caagccactc
     1801 cttggaaacc tgtcgccgtg ccaggaccag aggcgcaacg gacgactgga accgcgaaag
     1861 cgtccgctgg atttgtaaca gacctcggat gaataccgta ctatgccaaa gacaaagtat
     1921 gaggataccc gcagcaccga tccagctgag cataggactc tgaagcccgc cgacaaccgt
     1981 cacatcgcga gtgagtaatt tccaaagggc ggtgaacgaa tccaattcat ccatcggaca
     2041 aacctcacgg ggaccctaac gccatctcag caagagcaga actcgatcgt ctccctcaac
     2101 ttctgtgcca gagagggaat gcgataaata gccgcgaatc gctatgctgt cgagataact
     2161 ccgtacgagg atggaaattc ttgccgagaa ggaggcaatt tcggcaggga tgttccttca
     2221 ctccacggtc aagaagtaaa tttgtcctct agtcgattga aagttggttt tcgtgttatg
     2281 gtttgatgtg ccttttgttt tggaatcaaa cagaaggtat tcaaaaggag gcggttccaa
     2341 tgttctcaag aacacgtttg agtcgactta ctggaattat gtgcgtggtg tgctcggtaa
     2401 cgggatgtgc aagcgaattc tctgtt
//
LOCUS       DASTWE010000004         1449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_290984, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000004 DASTWE010000000
VERSION     DASTWE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290984"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFT30_00030"
     CDS             complement(<1..474)
                     /locus_tag="VFT30_00030"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5091038.1"
                     /translation="MMKPFGRKRRRLSTVSTDLDLLEGLPLATLILDCDLTVLAVNRA
                     CHRLLGPRFPSSTVRSFPALWSMLTQSDTTTITIRLNGVLKNRRPTSARQHLLLRRTT
                     LPVPVEWTCAPSTFNGKTVLIITIRDLSHELELEQDRNRLAAIAEESPLPTIELDR"
     gene            complement(471..>1449)
                     /locus_tag="VFT30_00035"
     CDS             complement(471..>1449)
                     /locus_tag="VFT30_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5091039.1"
                     /translation="TFVAKPDDHSVTVIYAQAHEAVERIFEELEHGIPPTLEATKAIV
                     SNVLEQILSGRAAMATQVAIRKIKQFDRSLTAHALDTCILSLVVAIESGVEQPMLEQV
                     GIGALLHDAGYARLPRNLVRKREECTGQDKTLLEQHCKLGVTLLSEPSGMPEDVLRIV
                     SEHHERADGSGFPAGLGSDQISRLAQIVGIVNFYDGMVSRHGAHPAMIPHDAVRQLFL
                     AGERGHFEKSLVERMIRSIGVYPVGSLVKLNTGEQAVVVGVNPQQRLKPVVKVTTDPQ
                     GGSYTTPIEVDLAAPSPDRTVRTVLRVLDPARERVNIGIHLDQALSRAA"
BASE COUNT          311 a          383 c          425 g          330 t
ORIGIN      
        1 ccgatccagt tcgatcgtcg gcaagggact ttcctcagcg atcgcggcca accgattgcg
       61 atcctgctcc aattcaagtt catgggacaa gtctcgaatg gtgatgatca gtacggtctt
      121 tccattgaat gtactcggtg cgcaagtcca ttccacagga acggggaggg ttgtccgccg
      181 caagagaaga tgttgcctcg cagaggttgg acggcggttt ttcagtactc cgtttagtcg
      241 tattgtaatg gtggttgtgt cggactgggt gagcatcgac cacagggctg gaaaggatcg
      301 gacagtggaa gagggaaacc gtggtcctag caaccgatgg cacgcccggt taacggccag
      361 tacagtgaga tcacagtcga ggatgagggt cgccagcgga agcccttcaa gaagatcgag
      421 gtcggtggaa accgtagaaa ggcgtctgcg tttccggccg aacggcttca tcatgcggct
      481 cgtgagagag cttggtccag atgtatgccg atgttgacac gttcccgggc cgggtctagg
      541 acacgcagca cggttcggac tgtgcggtct ggagatggtg ccgccaggtc cacctcgatc
      601 ggcgtcgtat aagagcctcc ttgtgggtcg gtcgtgactt ttaccactgg tttgagtcgt
      661 tgttgaggat tgactccaac gacgaccgcc tgctcgcccg tattaagctt gactaggctt
      721 ccgaccgggt agactccaat gctccgaatc atgcgttcca cgagagactt ttcaaaatgt
      781 cctcgttccc cggccaagaa gagttgccga acggcgtcgt gcggaatcat ggccggatgt
      841 gcaccgtgcc gactgaccat gccgtcataa aaattgacga tgccgacaat ctgtgcgagg
      901 cgtgaaatct gatcgctgcc tagaccggct ggaaatccgc ttccatcggc ccgctcatga
      961 tgctcactaa cgattcgtaa gacgtcctcg ggcatgccgg aaggctcgga cagaagcgtc
     1021 acgcccaact tacaatgctg ttccagaagt gtcttgtctt gtccggtgca ttcctcccgt
     1081 ttgcgaacca gatttcgtgg taggcgagca tacccggcgt cgtggagtaa cgcacctatg
     1141 ccgacttgtt cgagcatcgg ttgctccacc ccgctttcaa tggccacgac aagggacaag
     1201 atacaggtat ccagtgcatg agccgtcagt gaacgatcga actgcttaat tttccgaatg
     1261 gcaacttgag tggccattgc ggcgcggccg ctgaggattt gctccagcac attggagacg
     1321 atggccttgg ttgcttctag cgtcggagga atcccatgtt caagctcctc gaaaatacgt
     1381 tcaacagctt cgtgagcttg cgcatagatg accgtgacgg aatgatcgtc aggctttgcc
     1441 acaaacgtc
//
LOCUS       DASTWE010000005         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1422065, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000005 DASTWE010000000
VERSION     DASTWE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422065"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VFT30_00040"
     CDS             complement(<1..117)
                     /locus_tag="VFT30_00040"
                     /inference="COORDINATES: protein motif:HMM:NF018158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091040.1"
                     /translation="MNKFIFVTGGVVSSLGKGLASASIGNLLESRGLKITFLK"
     gene            complement(167..967)
                     /gene="kdsB"
                     /locus_tag="VFT30_00045"
     CDS             complement(167..967)
                     /gene="kdsB"
                     /locus_tag="VFT30_00045"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU5091041.1"
                     /translation="MPKTSRSVTLVIPARYGSSRFPGKPLVKLNGKPMIQHVYERAVA
                     SRAVSDVLVATDDERIKQAVERFGGRVIMTTGDYRTGTDRVAAVARMFAGEYFLGLQG
                     DEIPLNSELLTDLIEPFLESGAGMGTLKRVMDPTEDLLNSAVVKVVTDAGGNALYFSR
                     SPIPFVRDDPEMQAMGGLHYIHLGLYIYTKETLLRFAALPTSRLEEAEKLEQLRALDH
                     GVRIRVWETKHASLRVDRPEDVPHVADRLQQFAAIKRELENSGMFFKP"
     gene            complement(960..>1553)
                     /locus_tag="VFT30_00050"
     CDS             complement(960..>1553)
                     /locus_tag="VFT30_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5091042.1"
                     /translation="RSELKAALRQKIVRYVESRIGDLSCIVISDYAKGVVSPTLMSEI
                     TRLAASRKVPIIVDPKVEHFSYYKGVTVLTPNHLEAAQASGLHGDSDQTIDEAGAMIR
                     QRLGCRSVLITRGEKGMSLYEDDGASWHLPTKARQVYDVTGAGDTVIGTLALALSVGA
                     SIKIGAIMANYAAGIVVGMVGTATVSPKQLSEAFGDA"
BASE COUNT          326 a          476 c          410 g          341 t
ORIGIN      
        1 cttgagaaac gtgatcttca atccccggct ttcaagcagg ttcccaatgg aggccgaggc
       61 cagtcctttt ccaagcgatg agactacccc gccggtcacg aagataaact tgttcatggc
      121 tgccctctct cctggacgtc agaccgctcc gatccgaata tgaatctcat ggtttgaaaa
      181 acattccgct gttctccagc tcccgcttga tcgccgcaaa ctgctgcagc cgatccgcga
      241 catgaggcac atcttccggg cgatcaaccc gcagagaagc gtgtttggtc tcccacacgc
      301 ggatgcgaac accgtggtcc aatgcgcgaa gttgttcgag tttttcggcc tcttccaagc
      361 ggctcgtcgg cagagccgcg aatcgaagca acgtttcctt cgtatagatg tacaacccta
      421 gatgaatata atggagccct cccatcgctt gcatttccgg atcatcgcgg acgaatggga
      481 tcggcgatcg cgaaaagtac agtgcattgc ctcccgcgtc ggtcacaacc ttgaccacgg
      541 cggaattgag taggtcctcc gtggggtcca tcacacgctt gagcgtcccc atgccggctc
      601 cgctttcgag aaatggctcg atcaggtcgg tcaataactc ggaattcaag ggaatttcgt
      661 caccctgcaa gcccaagaaa tattctcccg caaacatacg ggccacggcg gcgacccgat
      721 ccgtgccagt ccgatagtcg cctgtggtca tgatcacgcg cccgccaaac cgctcgacag
      781 cttgcttgat acgctcgtcg tctgtggcga ccaatacatc ggaaacggca cgggaagcca
      841 cggcccgctc atacacatgt tgaatcatcg gcttgccgtt cagcttgacg agcggcttgc
      901 ccgggaaccg tgacgagcca tatctggctg gaatcacaag ggtgactgac cgtgaggttt
      961 taggcatcgc caaacgcctc ggacaactgt ttgggcgaga cggttgcggt gccgaccatt
     1021 cccaccacga tcccggccgc gtagttcgcc atgatcgctc caatcttgat acttgccccg
     1081 accgacaagg ccagcgcaag agttccgatg accgtatctc ccgccccggt gacgtcgtag
     1141 acttgccgag ccttcgtggg cagatgccat gatgcgccgt cgtcctcgta caagctcatg
     1201 cctttttcac cgcgcgtaat caggacggac cggcatccaa gacgctgtcg aatcatcgct
     1261 ccggcttcgt cgatcgtctg atcgctgtcg ccgtgtaaac cggaggcttg agccgcctcg
     1321 agatgatttg gtgtgaggac tgtgacacct ttgtagtagc tgaaatgttc gaccttcgga
     1381 tcgacgatga tcggaacctt ccgcgaggca gcgagtcgtg tgatttcgga catgagtgtc
     1441 ggcgacacga ctcccttggc gtagtccgat atcactatgc aggacaaatc cccaatcctt
     1501 gactctacat agcggacaat cttttgccgc agcgctgcct tcagttcgct ccg
//
LOCUS       DASTWE010000006         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_808084, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000006 DASTWE010000000
VERSION     DASTWE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808084"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFT30_00055"
     CDS             complement(<1..980)
                     /locus_tag="VFT30_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5091043.1"
                     /translation="MTKGVTQIRRSVMVLAFPVTVSSLLQRAEGIVAVFLVGGLGATS
                     IAAVGLGQLLAFVATTLVSGISVGTNVIVAQLWGARRRQDAGEAARHFLWLSIGVSLV
                     LASLGMAGNRFVLEQLGAEPVVIELALPYSTLIFFVIPCTMLIQVLSSILQGTGDTRT
                     PMYAMIGVNLLHVLLAYPLIYGRWGIPPLGLKGAAIAVGLAEATGTLYLLLRCRPILK
                     SSSTLRLDLIRSIWDVGASVSGERIVQQAGIFIYTKLVLLYGTVAYAAHQVGLSIESF
                     SFLPGYGLAIAAATMVGQSIGAGKYARAKLENWEANRIAIVLMTCMGIIF"
     gene            1049..>2322
                     /locus_tag="VFT30_00060"
     CDS             1049..>2322
                     /locus_tag="VFT30_00060"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEU5091044.1"
                     /translation="MTTTDVHNGSPTTFAPPRLSKSKFLSGLQCHKRLYLEIHQPLLA
                     TPPDASTQAILDMGTEIGILAQRRFRDGVLVKAGFRQREAAIAETAALLQDSRIPAIF
                     EAAFEHEGVLVRVDILERVQNGEGISSSWRLIEVKSSTRVKDVHLDDLSIQSYVVQGA
                     GLKLDAICLMHIDTRYLYEGGEVDLQALFSIEDVSESVASRRGQMLERLAAMRVMLLN
                     SQPPEIEPGQHCHAPYECPFWSHCTTQKSSRWIYHLPGKKEIVGQLVQQGITTIDEIP
                     DGTQLSDIQRKVKDNVEWISSELSPILRSVRYPVHHLDVETVMLALPRFLSTRPYQPI
                     PVQWSNHIELESGDVSHQEFIHNEASEPRRRWAEALIESLGEKGSIVVYSAYEEAIIR
                     QLAETFPEFKSAFKAIVKRLWDLLPVIKNHYY"
BASE COUNT          570 a          628 c          635 g          489 t
ORIGIN      
        1 aaaaaaatga tccccatgca ggtcatcaga acaatggcga ttcgattggc ctcccagttc
       61 tccagtttgg ccctggcgta ctttcccgct ccgatgcttt gccccaccat ggtcgctgcg
      121 gcgatggcga gtccgtaccc aggcaaaaat gaaaacgatt cgatcgacaa gccgacttgg
      181 tgggcggcgt atgcgacggt gccgtacaac aagaccagct tggtgtagat gaaaattccc
      241 gcctgttgga cgatccgttc gccggacacc gatgctccga catcccagat cgatcggatc
      301 aagtcgagac ggagcgtgga ggatgatttt aagatgggcc gacaccgcaa cagcaggtag
      361 agagtaccgg tcgcctctgc gagaccgacg gcaatggctg cgcccttgag acccagtggc
      421 gggattcccc atcgcccata gatgagagga taggcaagaa gaacatgcag aagattaacg
      481 ccgatcatgg cgtacatcgg agtgcgcgtg tcacccgttc cctggagaat tgaggacaag
      541 acttggatga gcatggtgca cgggatcaca aagaagatga gtgtcgaata ggggagggca
      601 agctcaatga cgacaggctc cgcacccagt tgttccagca caaagcggtt gccggccatc
      661 ccaaggctgg ccaaaaccag cgacacgccg atcgaaagcc agaggaaatg cctcgcggcc
      721 tctcccgcgt cctgacggcg tctggccccc cacagctgcg cgacgatgac attcgtgccg
      781 actgaaattc cagagaccaa ggtcgtcgcg acgaaggcca gaagttggcc caacccaacc
      841 gctgcgattg aggttgcccc cagaccgccg actaagaata ctgctacaat gccttcggcc
      901 cgctgaagga gactgctgac ggtgacagga aatgccagca ccatcacgga gcgtctgatc
      961 tgagtgacac ctttggtcat gaggcgctat cctaacagag ttgctcctcg taccatctga
     1021 gaaaatggct gattacaccg tggactgtat gacgacgacc gacgttcaca acggatctcc
     1081 cacgacgttc gcaccacccc gcctgtcgaa atcgaaattc ctttcgggtc ttcaatgtca
     1141 caagcggctc tacctggaaa ttcatcagcc cctcttagca acccctcctg atgcgtcgac
     1201 gcaggcgatc ttggacatgg gaactgagat cgggatcctg gcgcagcggc gctttcgtga
     1261 tggcgtactt gttaaagcgg gattccgtca gcgagaggcg gcgatcgcag agaccgctgc
     1321 cttactgcaa gattccagaa ttcccgccat cttcgaagcg gcattcgagc acgagggagt
     1381 tcttgtgcgc gtcgatattt tagagcgagt gcagaatggt gaggggatat cgtcatcttg
     1441 gcgtttgatc gaggtgaagt cctcgacaag agtgaaagac gtccatctcg acgatctgtc
     1501 cattcaaagc tacgtcgtac aaggtgccgg gttgaagctc gacgcgatct gcctgatgca
     1561 catcgatacg aggtatctct acgaaggcgg cgaggttgat ctgcaagcac ttttctcaat
     1621 tgaagacgta tcggaatctg tggcaagtcg acggggacag atgctcgaac ggttggctgc
     1681 tatgagagta atgttgttaa actcgcaacc accggagatc gaacctggtc aacattgtca
     1741 cgctccctat gaatgtccct tctggtcaca ttgcacaacg cagaaatcgt cgcgctggat
     1801 ctatcacctg cctggtaaaa aagagatcgt cggtcagctc gtgcaacagg gcatcacgac
     1861 gatcgacgaa attccagatg ggacacagct gtctgatata caacgaaaag tcaaagacaa
     1921 tgtcgagtgg atttcgtcgg aattgagtcc aattcttcgc tcagtgagat atcctgtaca
     1981 ccacctggat gttgagaccg tcatgttggc actgccgcga tttctttcca ccaggcccta
     2041 tcagcctatt cctgttcaat ggtccaatca cattgaacta gaatccggcg acgtgtctca
     2101 ccaagagttt attcacaatg aggcatcgga gccgcgcagg cgttgggccg aggccttgat
     2161 cgagtcgctc ggcgagaaag gcagcatcgt tgtgtattca gcctatgagg aggccatcat
     2221 acgacaactt gcagagactt ttccggagtt caaatccgcg ttcaaagcga ttgtgaagcg
     2281 gttgtgggac ctgctccctg ttatcaaaaa tcactattat ca
//
LOCUS       DASTWE010000007         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_872726, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000007 DASTWE010000000
VERSION     DASTWE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872726"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1080
                     /locus_tag="VFT30_00065"
     CDS             <1..>1080
                     /locus_tag="VFT30_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5091045.1"
                     /translation="KKNFEAVDATVANLYEVNVPSVITSKTSRRLSVPSEAPEFVRDV
                     LGEIIGANGDNIPVSAFPVDGTFPTGTTQWEKRNIALEIPVWDEKLCIQCGKCVMVCP
                     HAVIRHKVYDEKLLASAPETFKSMPSKFKEFSKGYAYTVQVAPEDCTGCTLCVEVCPV
                     KDKTQVGRKAINMEAQPPLREQEAKNWDFFMEIPDLDRKLINPSTIKNSQLLRPLFEF
                     SGACAGCGETPYVKLVSQLFGDRSVIANATGCSSIYGGNLPTTPWAVDSKGRGPAWSN
                     SLFEDNAEFGLGMRLTIDKQGEYARELLASFEKELGKEFIDSILNADQADEAGIDAQR
                     ERVAELKNKLGKSSDSRSKDLVSLA"
BASE COUNT          263 a          293 c          291 g          233 t
ORIGIN      
        1 tcaagaaaaa ctttgaagcg gtggatgcaa ccgttgcaaa tctgtatgaa gtaaacgtcc
       61 cgtctgtaat cacatccaag acctcgcgcc ggctctccgt cccgagtgaa gcaccggaat
      121 tcgtccgcga cgtacttggc gagatcatcg gcgcgaacgg cgataacatc cctgtatccg
      181 ccttccccgt agatggtacg ttcccgacag gcaccacaca atgggagaag cgcaatatcg
      241 cgctggaaat cccggtgtgg gatgaaaaat tgtgtatcca gtgcggcaag tgtgtgatgg
      301 tttgtccgca tgcggtcatc cgccacaaag tatatgacga gaagttgctg gcaagtgcgc
      361 cagaaacctt caagtccatg ccttcgaaat tcaaggaatt ctcgaaaggt tatgcttaca
      421 cggtacaggt cgctccggaa gactgcacag gttgtacctt gtgcgtggaa gtctgcccgg
      481 taaaggacaa gacgcaggtc ggacgcaagg ccatcaatat ggaggcacag ccgccgctcc
      541 gtgaacagga agccaagaac tgggacttct tcatggaaat ccccgacctt gatcgcaagt
      601 tgatcaaccc atcgaccatt aagaattctc aattgctccg cccgttattc gagttcagtg
      661 gcgcctgtgc tggctgtggc gagacgccct atgtgaaact ggtctcgcaa cttttcggcg
      721 atcgctctgt gatcgccaat gcgaccggtt gctcttccat ctatggcggc aacctcccca
      781 ccaccccatg ggctgtcgac tccaagggac gtgggcccgc ctggtcgaat tccctgttcg
      841 aagataacgc tgaatttggt ctcggcatgc gactgaccat cgacaagcag ggcgaatatg
      901 cccgtgaact gctcgcctcg ttcgaaaagg aactcggcaa ggaattcata gattcgatcc
      961 tgaatgccga tcaggctgat gaggctggca ttgacgccca gcgcgaacgc gtggctgagt
     1021 tgaaaaataa acttggcaag tcaagtgact cacgttccaa agatttagtc agcctggcgg
//
LOCUS       DASTWE010000008         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1357460, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000008 DASTWE010000000
VERSION     DASTWE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357460"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1057
                     /locus_tag="VFT30_00070"
     CDS             65..1057
                     /locus_tag="VFT30_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEU5091046.1"
                     /translation="MSLKHIFEEHPERRYIMFGGKGGLGKTTFSAATAYWLAQQGKRV
                     LVFSVDPQASLSDIFQQDIFGKGPVKIMENLWAQEIDADQHIKAYQNEIRQKILDMYG
                     FDQVPDEIEQYIQAASAEPAMEESAIFDAVVDIVVQGDYDYYIYDLVPLGHALYYLSM
                     AKVYDEWINKITKLREEMREYEEMVSRLKREKETEEDQILKELQYIRGRINASSQILT
                     DKEKTGFFFVVIPEEMILIDTAKAAELFAKFDVPIMGYVVNRVVPRELLNQNIPPYLK
                     NRIEMQDKYLKQIDETFGKQVASRVPELERDVTGLEMIKKLSEIMYGEGVGETL"
     gene            1054..>1233
                     /locus_tag="VFT30_00075"
     CDS             1054..>1233
                     /locus_tag="VFT30_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEU5091047.1"
                     /translation="MIAKAIETRMKDYVKEHDQLRYIFFGGKGGVGKTVMAGATAIWL
                     AKQGKRVMLASTNPVH"
BASE COUNT          336 a          305 c          343 g          249 t
ORIGIN      
        1 aaacaggtac gcacgtgggt atgggtctac ttgcatgcta atctacctcc tataaggaga
       61 catcatgtcc ctcaaacaca tattcgaaga acatcctgaa cgacgctata tcatgttcgg
      121 ggggaagggt gggctgggaa agacaacctt ttccgccgcc accgcctact ggcttgccca
      181 acaggggaaa agggtgctgg ttttttcggt cgatccacag gcaagtcttt ccgatatttt
      241 ccagcaggat attttcggca agggtcctgt aaaaatcatg gagaatctct gggcgcagga
      301 gatcgacgcc gaccagcaca tcaaagccta tcaaaacgaa atccggcaga agattctgga
      361 catgtacggg ttcgaccagg taccggatga gatcgaacag tacatccagg ctgcctccgc
      421 cgagcccgcc atggaggaaa gcgccatctt cgacgccgtt gtggatatcg tggtccaggg
      481 cgattacgac tactacatct atgatcttgt ccccctggga catgcgctct actacctgag
      541 catggcaaag gtctatgatg agtggatcaa caagatcacg aaactgcgcg aggagatgcg
      601 ggaatacgag gagatggttt cacgattgaa gcgggaaaag gaaacggaag aagaccagat
      661 cctcaaggaa ttacagtata tccgggggcg catcaacgcc tcgtcccaga tcctgaccga
      721 caaggaaaag accggcttct tctttgtggt catcccggag gaaatgattt tgatcgacac
      781 cgccaaggct gccgaactct tcgcaaagtt tgacgtgccg atcatgggct atgtggtcaa
      841 ccgcgtggtg ccgcgcgagc tgctcaacca gaatattcca ccctacctga agaatcgcat
      901 cgagatgcag gataaatacc tgaagcagat cgatgaaacg ttcggcaagc aggtcgcctc
      961 acgcgttcct gaactggaac gggatgtgac cggcctggag atgatcaaga aactatccga
     1021 gatcatgtat ggggagggag taggagaaac gctatgatcg ccaaagcgat tgaaactcgc
     1081 atgaaagact atgtgaagga acatgaccag ctacgctaca tcttcttcgg cggcaaaggc
     1141 ggcgtaggga aaacggtcat ggcaggggca accgcgatct ggcttgctaa acagggcaag
     1201 agggtaatgc tggcgtcgac caatcctgtc cat
//
LOCUS       DASTWE010000009         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1519136, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000009 DASTWE010000000
VERSION     DASTWE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519136"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFT30_00080"
     CDS             <1..914
                     /locus_tag="VFT30_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEU5091048.1"
                     /translation="DENLLNDARKRLRHDNQRLDELMTDLQRKQRQMAEDNEKAQSAR
                     QEAEQAAREAQRLRAQLQEAEQEVRRGLKKKLGEQFQRARAEVEATVDSLKREQKLIK
                     AKETKQRLHELEMKVRQDLVPAGKPIPLEQLGIGDAVEIVGLGMTGNLLEMPQGKKRV
                     RVKVGEGEILATVSNLVGLAGESAPAPPPTAPSESDRRFPTGGGLGLDEQTVVDVRGQ
                     AADEALDQVVAALDRATLSGTPYLRIIHGHGTGRLKSVLREYLKESPYVTEFRPGDRA
                     EGGDGVTVARLRGREMRGPIPCLNAKS"
     gene            1053..>1142
                     /locus_tag="VFT30_00085"
     CDS             1053..>1142
                     /locus_tag="VFT30_00085"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU5091049.1"
                     /translation="MTFQELILALHRFWADQGCVIHQPYDMEMG"
BASE COUNT          291 a          276 c          349 g          226 t
ORIGIN      
        1 tggatgaaaa tctattgaac gatgcgcgga agcggttgcg tcacgacaat caacggctcg
       61 atgagttaat gacggattta cagcggaagc aacgccaaat ggctgaagat aatgagaagg
      121 cccagagcgc tagacaagaa gccgaacaag cggctcggga agcacaaagg cttcgagcgc
      181 aactgcaaga agcagagcaa gaggtccggc gtgggctcaa gaaaaaactc ggcgaacaat
      241 ttcaaagggc tcgggccgag gtagaggcca ctgtcgactc cttgaaacgt gaacagaagc
      301 tgatcaaggc taaggagacg aaacagcggt tgcatgagtt ggagatgaaa gtcaggcagg
      361 accttgtgcc ggccggcaag ccgatcccac ttgagcaact tggtatcggc gatgcggtgg
      421 aaatcgtcgg gttgggcatg acggggaatt tgttggaaat gcctcaaggg aaaaagcgcg
      481 tccgtgtcaa agtcggggag ggcgaaatct tagcgacggt ctcgaacctt gtcggcctgg
      541 caggcgaatc ggctcccgca ccaccgccta ctgcgccatc ggaaagtgac cgtcggtttc
      601 caactggtgg tggattgggt ctcgacgagc agacggtggt ggacgtacga gggcaagccg
      661 cagacgaggc actcgatcag gtcgttgcgg cactggatcg ggcgactctt agcggtaccc
      721 cgtacctccg gattatccat ggtcatggga ccggccgact caaatccgtc ttgcgcgagt
      781 atctcaaaga gtcgccttat gtaaccgagt ttcgtcccgg cgatcgagcc gagggaggag
      841 atggagtgac cgtagcaagg ttgcggggaa gagagatgcg tgggccaatt ccttgcctca
      901 acgccaagtc gtaatagctc tttcgtcatg actctcaaat gcaagctatt gatctagagg
      961 gacagttcct tgacagaaga aaagcattct tgctagcttg gcagctctag agaacgcatc
     1021 taatcatctc tcccgaccag gctgtaggtt ccgtgacttt ccaagaactt attcttgccc
     1081 tccatcgatt ctgggcagat caaggttgtg tcatccatca accttatgac atggagatgg
     1141 gg
//
LOCUS       DASTWE010000010         1727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_452690, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000010 DASTWE010000000
VERSION     DASTWE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452690"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFT30_00090"
     CDS             complement(<1..477)
                     /locus_tag="VFT30_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091050.1"
                     /translation="MSDHKNHRHTLYLIDGSAYIYRAFFALPPLSNSKGFQTNAVYGF
                     TTMLLKIMREHRPDALAVVFDEKGPTQRHEEYQAYKAQRPAMPDAMSAQVPYIHRIVE
                     ALAIPAIRLAGHEADDIIGTLARKAERADRDVVIVTSDKDMFQLLTPGVRIYDPVKD"
     gene            complement(552..983)
                     /locus_tag="VFT30_00095"
     CDS             complement(552..983)
                     /locus_tag="VFT30_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEU5091051.1"
                     /translation="MDWLLSAVQQYVSTETLVTLTVLSVVFFVGSLIAIPFILVRLPT
                     DFFDTRVPRRWMENHHPVLRSLGHLVKNVVGAIFLFAGFLMLFLPGQGILTMLIGVTM
                     LDFPGKRKLEAKMIGQPAVLSAINSMRQKFGKPPLTIASGS"
     gene            1270..1695
                     /locus_tag="VFT30_00100"
     CDS             1270..1695
                     /locus_tag="VFT30_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091052.1"
                     /translation="MTTSTKPKFVIFAHNATYDKLHQVATLGMTAAAMGKDVIIVLLF
                     WTIKKLAEGKIDQIDFPPEYAESAEEVARLLKEKKVPKISEMFQDAKHVGTLRLIACS
                     AGLEYMGVDSSAVEQNVDEVLGLPAILSLTDDSETKLFI"
BASE COUNT          408 a          448 c          485 g          386 t
ORIGIN      
        1 atctttgacc ggatcataaa tcctcacgcc gggcgtgagc agctggaaca tatccttgtc
       61 gctcgtcacg atcaccacat cgcgatcggc ccgttccgcc tttcgagcca gtgtgccgat
      121 aatgtcgtcc gcctcgtgtc ctgctagtct gatggcggga atcgccagcg cttccacaat
      181 acggtgaata tagggtacct gcgcgctcat ggcgtccggc atcgcaggtc gctgggcctt
      241 gtacgcttgg tattcttcat gccgttgtgt agggcctttc tcatcgaaga cgacggcgag
      301 agcatcgggc cggtgctctc gcatgatctt caggagcatg gttgtgaacc catacacggc
      361 attggtttga aagcctttcg aattcgacag tggcggcaag gcaaagaagg cccgatagat
      421 gtaggcgctg ccgtcgatca ggtaaagcgt gtgacggtgg tttttgtgat cagacatact
      481 tcttcagcgt ctcccagcgg ttcgccgctt gtgccctagg ggtcgactgc tgatagatga
      541 tggcatgggc gttatgatcc cgacgcaatc gtgagcggcg gcttaccaaa cttctgacgc
      601 atgctattga tggcactcag gacagcgggc tgtccgatca tctttgcttc caacttgcgc
      661 ttgcctggaa agtccaacat cgtcacaccg atcaacatgg tgagaatacc ctggcccggc
      721 aagaacaaca ttaagaaacc cgcgaacaga aagatggctc cgacgacgtt ctttacgaga
      781 tggccgagcg accgaagcac cgggtgatgg ttttccatcc aacgccgcgg cacacgggtg
      841 tcgaagaaat ctgtcggaag tcgaacgagg atgaacggga ttgcaattaa tgatccgacg
      901 aagaagacaa ccgagagaac cgtcagggta acgagggttt cagtcgagac atactgctga
      961 accgccgaaa gaagccaatc catcggtcgg catcctaaca tgggcttcta cgcccctcaa
     1021 ggcgaagcag gtccttgtct gatgtttacg ccgtcgtccc tgatcgttat aatcatcggt
     1081 catgaggctt cggtatgaat cggtacaagc cgtcttcgaa ggtcgggaag gtcggatggt
     1141 ttctttttca tcttgatggc ggcggcctgg aacctttctc ttgtcgcatg atcgaccgat
     1201 gacagccgat gaagtcgagg cagcgatcac ggactacgtc aacagcgtga aaacccgcac
     1261 tcagaagaaa tgactacgtc gactaaaccg aagttcgtca tcttcgccca caacgccacg
     1321 tacgataagc tgcaccaggt cgcgacgctc ggtatgacgg ctgcggcgat gggcaaagac
     1381 gtgattatcg tcttgttgtt ctggacgatc aaaaaactcg ctgaagggaa gatcgatcag
     1441 atcgatttcc cgccggaata tgccgagtcg gctgaggagg ttgcccggct gcttaaagag
     1501 aaaaaggtgc cgaagatctc ggaaatgttc caagatgcca agcatgtcgg aacgctgcgg
     1561 ctgatcgcct gcagtgccgg attggaatat atgggtgtag acagttctgc ggtcgaacag
     1621 aatgtcgatg aagtgttggg cctgcccgcc attctttcat taaccgatga ctcggaaacg
     1681 aagctgttta tctaaggacc ggtgaaacga aaaacatcca tacctct
//
LOCUS       DASTWE010000011         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1454475, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000011 DASTWE010000000
VERSION     DASTWE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454475"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFT30_00105"
     CDS             <1..209
                     /locus_tag="VFT30_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091053.1"
                     /translation="GHCSLHLSELQLSLLCPFSALKPKGTEKGSPGESFSFHRPSSSL
                     SKEATARQECLPRNATEPSLFLKT"
     gene            544..1224
                     /locus_tag="VFT30_00110"
     CDS             544..1224
                     /locus_tag="VFT30_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5091054.1"
                     /translation="MTFKNREEAGRRLVERLMQYHGDPTAIILALPRGGAAVGCQLSL
                     GLHLPLDVFIARKLGAPDNPEYALGAVGETGTIYLNPDAIAVYNLSSQEIEDLAQAQQ
                     QEIARRQILYRQDRQLPILIDRTVILVDDGIATGSTFFASAKSIKHLKPRRLIGAIPV
                     GPVETIQEAHRQVDELVVLATPDPFWAVGNHYIDFAQISDHDVVEFLRLAEESLSQWK
                     ERTHSSTA"
     gene            1297..1845
                     /locus_tag="VFT30_00115"
     CDS             1297..1845
                     /locus_tag="VFT30_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091055.1"
                     /translation="MIVRRWGKRATIIVSLVLPFLTGLGTVQAKIVVPPSGSVIEVDT
                     ATLKAVVGLFETAEDAIKAHDLDKVMTMYSSHYNYHGLKKNDVRKIWKDLFDEYQEIA
                     STHLFTRMTKVGSGGDAMLEVTCTGHLWARSKISGLYIPIDSWHEEVHYLVLEEGTWR
                     IHGNGGESPRVLPFGTAPHPLF"
     gene            1870..2727
                     /locus_tag="VFT30_00120"
     CDS             1870..2727
                     /locus_tag="VFT30_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5091056.1"
                     /translation="MRVVIGVDWSDQAFAAVSQTFQLYHPTDVTLVHGVDLGILKHPF
                     VAQAGNVQGYDDFRHAMVDSGNQLLERAAAMVPVEVASIRKVNEIGSPAQLILDSADT
                     LSADLVIVGVRGRSRLSEVVLGSVSHRVLLHGSRSVLIVRGVARKIQRVLVAIEDRDD
                     ADRITKWLTHHPFTDPTELCVLHALVPIGISDPYDALGTGTWWEGAERYADELVKSTA
                     GKLLNPRYTVNTKVATGNPAAVIEQEAKDRDLVVVTSHGRKGLSRFLLGSVSHAVVHH
                     VTCPVLVVR"
BASE COUNT          665 a          779 c          788 g          683 t
ORIGIN      
        1 acggtcactg ctcgcttcac ctttctgaac tgcaactaag cttgttatgt ccattttccg
       61 ccttaaaacc caagggaaca gaaaaaggtt cgccaggaga atctttcagt ttccaccgcc
      121 catcgtccag tctgtcgaaa gaggcgacag cccgtcaaga atgtttgccc cgtaatgcta
      181 cagagccttc tcttttcctg aagacctgac gttgaccggc ttatcttaaa acgccatgtt
      241 tttcatgcat tcttgcgcgt ttggcactgg cactgtcgat gcacatcttc acgggatggg
      301 gctaaaagtg ctctaccaaa ggcggagaag gcccaagccc aaaacgttga agggaggggc
      361 atgatgtttc gccatcccgt cgcctcgaaa ttccctgaga agtccattgc gatcaaagga
      421 aatcggaccc catcatcgtt tgctgctcat tctcaccctg atctttcata gcgatgttct
      481 tgatgatggg tatgacagtg gcctgatgta gacctgtgag cccagtgaaa ggaatggaga
      541 agcgtgacgt tcaagaaccg agaagaggcc ggtcgtcggc tcgttgagcg attgatgcaa
      601 taccatggcg atccaacagc cattattttg gcgcttccac gtggtggggc ggctgtcggt
      661 tgtcagttaa gtctagggct gcatcttcca ctggacgtct tcatcgctcg aaagctggga
      721 gcaccggaca atcctgaata tgcgctgggc gctgtgggtg aaacagggac aatctacctg
      781 aaccctgacg caatcgccgt ctataatctt tctagccaag aaatcgagga cctggcgcag
      841 gcacaacaac aagagatagc tcgacggcaa attctctatc gacaggaccg acagctccct
      901 atactcatcg accgaactgt cattctcgta gatgacggaa tcgccaccgg ttccacattc
      961 ttcgcctcgg ccaaatccat caagcacctg aagccccgcc gcctgattgg tgccattcca
     1021 gtcggtccag tggagacgat acaggaagct cacaggcagg tggacgagtt ggtggtcctt
     1081 gccacaccgg acccgttctg ggccgtcggt aatcactata tcgactttgc acagatcagc
     1141 gatcacgatg tggtggagtt tttgaggctg gcggaagagt cgctgtcgca atggaaggaa
     1201 cggacacact cttcaacggc ctaaccgccc ttgttaaggg aatgcgcagg cagcgaaaat
     1261 gaagacaccg atgacagatc gacggagaga ggggtaatga tcgttcgaag gtggggcaaa
     1321 cgtgccacca tcattgtttc tttggttctt ccttttctta ccggtctagg aacggtgcag
     1381 gcaaaaatcg tcgttcctcc ttccggatcg gtgatcgaag tggacactgc cactctcaaa
     1441 gccgttgtcg gcctcttcga aacggctgaa gacgcaatca aagcccatga ccttgacaag
     1501 gtcatgacca tgtattcttc acattacaat taccatgggc tcaagaagaa cgatgttcgt
     1561 aaaatctgga aagacctctt tgatgagtat caagagattg caagcaccca tctcttcacg
     1621 aggatgacca aggtcggatc aggcggggat gcgatgctgg aagtgacctg taccggtcat
     1681 ctctgggcca ggtctaaaat cagcggtctg tatataccca tcgatagctg gcatgaggaa
     1741 gtgcattacc tcgtcctcga agaggggacc tggcgcattc atggcaacgg gggtgaatca
     1801 ccacgcgtgc tcccgtttgg taccgcgcca catccgctgt tttgaggttc gaatgggaaa
     1861 ggccatgata tgagagtcgt catcggtgtc gattggtcgg atcaggcttt cgctgccgtc
     1921 agtcagacat ttcaactcta tcatccgacg gacgtcacgc tggttcacgg cgtcgattta
     1981 ggaattttga agcatccatt cgtggcccaa gcaggcaacg tgcagggata cgatgatttc
     2041 cgtcatgcga tggtggattc ggggaaccaa ctgctggagc gcgccgccgc catggtaccg
     2101 gtggaggttg cgtcgatcag gaaggtgaac gaaatcggca gtcctgctca gcttatcctc
     2161 gacagcgccg acaccctctc agccgacttg gtcattgtcg gtgtgcgcgg acgaagccgt
     2221 ctttccgaag ttgtcctggg gagcgtgtcc catcgtgtac tcttgcacgg ctctcggtcg
     2281 gttctcattg tcagaggagt tgctcgcaag attcagcgag tgctcgttgc catcgaggat
     2341 cgagatgacg ccgaccgaat cacgaagtgg ctgacgcatc atccctttac cgaccccacc
     2401 gagctctgcg tgctgcacgc tctcgttccg atcggaataa gcgaccccta tgacgctctg
     2461 ggaaccggaa catggtggga gggcgcggag cgctatgcgg atgagctcgt caaatcaacc
     2521 gccggcaaac tcttgaaccc tcgctatacg gtgaacacga aggtcgccac gggcaatcct
     2581 gcagcggtga ttgaacagga ggccaaagat agggatttgg tggtcgtgac ctcccatgga
     2641 cgcaagggcc tttctcgttt cctcttgggg agcgtgtccc atgctgtcgt gcatcacgtc
     2701 acctgtcctg tcctcgtcgt gcgatgaagg gctgaacatg aaaaaggcat tgatcgctgc
     2761 ttcacgctca tatccgcgtt gatctttgct cgaatgatcc gacatcagct agtccaaggt
     2821 actatctgca tccttcaatt gaagttctcg gagtgtaatg cggttcgttc gccctctcta
     2881 cattccttcc tttgagatcg atcacactga atccg
//
LOCUS       DASTWE010000012         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_517323, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000012 DASTWE010000000
VERSION     DASTWE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517323"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFT30_00125"
     CDS             complement(<1..626)
                     /locus_tag="VFT30_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEU5091057.1"
                     /translation="MITMNTIYTYQIEGIALQTGDIICTMNGKPDILPGEFWRFVGRL
                     VPGDVDHVAIYIGPNGRCIEAGAHGVIKFTVFDEQWNTERMARRRGMLFDTFYGVASP
                     LDGLAASEEEEHEMRRAVAEYCLGQVGKPYNLNFLNAETEDAFYCSQLVYKAYQKIGI
                     DLNTGLAMEQLPGTNAIIYPQEIWDGFSHRLAEREQPAITSSALLTNPS"
     gene            complement(626..>1020)
                     /locus_tag="VFT30_00130"
     CDS             complement(626..>1020)
                     /locus_tag="VFT30_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5091058.1"
                     /translation="HLSSIVFLHGPLDREPLVEKLRQAHLLVVPSSYEGFGIVYLEGM
                     CFGLPAIGTRAGAANEIISHGVDGFLIQPEDADELAKKLKDLHENREMLIQMSLAARD
                     RYLRQPNWEETAGRIREFLLKRIDEFSV"
BASE COUNT          224 a          291 c          263 g          242 t
ORIGIN      
        1 gacgggtttg ttaacagcgc gctgctcgtt atcgcaggct gttcgcgctc cgcgaggcga
       61 tgactgaatc cgtcccagat ttcctgggga taaatgatag cgttcgtgcc gggaagctgc
      121 tccatcgcga gcccggtgtt caggtcaata ccgattttct ggtatgcctt atataccagt
      181 tggctgcagt agaaggcatc ctcagtctcg gcgttcagaa agttcagatt atacggctta
      241 ccgacctgcc ccagacaata ctctgcaacc gctctgcgca tttcatgttc ttcctcttct
      301 gaggcggcga gcccatcgag cggagaagcg actccgtaaa aggtatcgaa caacatcccg
      361 cgccggcgcg ccatgcgctc ggtattccat tgctcgtcaa agaccgtgaa tttgatcaca
      421 ccgtgggcac ctgcctcgat gcaccgaccg tttggcccga tataaatcgc cacgtggtcc
      481 acatcaccgg ggacaagccg gccgacaaac cgccagaact cgcccggcag gatatccggc
      541 ttgccgttca ttgtgcagat aatgtcgccc gtctgtaagg caatcccttc gatctgatac
      601 gtataaatcg tattcattgt aatcattata cggaaaactc atcgattcgt ttcagcagaa
      661 actcacggat ccgacctgcc gtttcttccc aattcggctg gcgaaggtaa cggtcccgcg
      721 ctgcaaggct catttggatc agcatttccc gattttcatg cagatccttc aattttttcg
      781 ccagttcgtc ggcgtcttct ggctggatca ggaaaccatc cactccgtgg ctgatgattt
      841 cgtttgccgc gcctgcgcga gtcccaatcg cgggcagacc gaaacacatt ccttccagat
      901 aaacgatccc gaagccctca taactggatg ggacgacgag gaggtgggct tgtctgagct
      961 tttcgacgag aggttcccgg tccaggggtc cgtgaagaaa gacaatggaa gaaagatggc
//
LOCUS       DASTWE010000013         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1131445, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000013 DASTWE010000000
VERSION     DASTWE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131445"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1080
                     /locus_tag="VFT30_00135"
     CDS             <1..>1080
                     /locus_tag="VFT30_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091059.1"
                     /translation="LVGGSFSGNIVGTPNWINANARVWLQNNNTVPGDFNFRWGIQIR
                     IPANVAGGILNTNGPELATDFDMWYLIRGSVMGMPTILAEYRTDGVNTTAFDLTNANY
                     PVPAAVPSGMPSDVWDRFQLTSGPASFGGVTIMGNGQNDLVVQNSTGTGTTIKSGEVN
                     TFIARPRNYRPNVPANEIAIGDIAATFRIANWGSVGGNPNQVNWASGTWDYVTGNPTP
                     LASTGAIPPLNAGNNPPATSPIAVPVTMTLGPNQSIHQCILCNLSSAGPAMNFLQDSV
                     FRNMDYATASTEERDAEINTIGLPKDDAPDPRDVYIAIEKVNMPINSPGADAGTFLST
                     NMLSVLRRGGPLAEKLRKVLEILRN"
BASE COUNT          256 a          302 c          292 g          230 t
ORIGIN      
        1 aattagtcgg aggctcgttc tccggtaaca ttgtcggcac tccgaattgg atcaatgcga
       61 atgcacgagt ctggctgcaa aacaacaata ccgtacccgg tgactttaat ttccgttggg
      121 gcattcagat tcgtattccc gcgaacgttg cgggaggcat tctcaacacc aatggtcccg
      181 agctagccac tgatttcgac atgtggtacc tgattcgcgg ctccgtaatg ggaatgccca
      241 ccatcctggc tgagtaccgc acagacggag tgaataccac ggcattcgac cttacgaacg
      301 ctaattatcc tgtgccggca gctgtgccgt caggtatgcc ctccgatgtg tgggatcggt
      361 tccagcttac ttctggtcca gccagtttcg gcggcgtgac catcatgggc aacgggcaga
      421 acgatctcgt ggtccagaac agcaccggca ccggcactac aatcaaaagt ggcgaggtga
      481 atacgttcat tgctcgacca cgcaactacc ggccgaatgt acctgcaaat gagatcgcca
      541 tcggagacat tgctgcaacg tttcgcattg ctaattgggg atcggtcggc ggcaacccga
      601 atcaggtcaa ctgggcctcc ggcacgtggg actatgtaac aggaaatccg actccactcg
      661 ccagtaccgg cgcgattccg cctttaaacg cgggtaataa tccaccagcg acaagtccga
      721 tcgcagttcc tgtcacgatg acgctgggtc cgaaccagag tatccaccag tgcattctgt
      781 gcaacttgtc gagcgctggt ccggccatga attttcttca ggacagtgtg ttcagaaaca
      841 tggactacgc caccgcttcg accgaggagc gggacgctga gataaacact atcggcctgc
      901 caaaagatga tgctccggat cctcgcgacg tgtatatcgc gatcgaaaaa gtcaacatgc
      961 cgatcaactc gcccggggca gacgcaggca cgttcctgag taccaacatg ttgagtgtcc
     1021 tgagacgggg tgggcctctt gccgagaaat tgcggaaggt gctcgaaatt ctcagaaacg
//
LOCUS       DASTWE010000014         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_937560, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000014 DASTWE010000000
VERSION     DASTWE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937560"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFT30_00140"
     CDS             complement(<1..369)
                     /locus_tag="VFT30_00140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5091060.1"
                     /translation="MNLRSPTMEDAQVVHGSGVVEELASRFGPDGLLAFQPTRDEIPT
                     MWLDRSRLRETLQYLRAEIPRPFPMLFDLSATDERLRRNHDGLPIGTFTVFYRLFSFA
                     RNQSLRIKVPLRDEPHSLPSI"
     gene            complement(366..1076)
                     /locus_tag="VFT30_00145"
     CDS             complement(366..1076)
                     /locus_tag="VFT30_00145"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5091061.1"
                     /translation="MRAWHNGRLEGVSIRDGGDGSLTSVVGQSVMLAKLQDLLAWGRK
                     NSLWPLNFGLSCCYVEMATSLTSVYDVARFGAEVVRGSPRQADVLVVSGTVFIKVAPI
                     IKQLYEQMMEPRWVISMGSCANSGGMFDAYSVVQGVDKFLPVDVYMPGCPPPPHAFME
                     CLLLLQDKVGKERRPLSWWFGPQEVQKPIMPSMRDAKREERVRLREYPTVDILPRTDL
                     LSPHFAGQPLDRPTTPKP"
     gene            complement(1060..1515)
                     /gene="ndhC"
                     /locus_tag="VFT30_00150"
     CDS             complement(1060..1515)
                     /gene="ndhC"
                     /locus_tag="VFT30_00150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5091062.1"
                     /translation="MALQWIFQIGLYAAGVFIVIGVMLGLPALLGERHWRKSERRREI
                     ATGVPYESGIRPTGSAQIRLPIQYYLVAMFFVIFDLEAVFLYAWAVAVKETGWLGFIE
                     VVIFVTILLAALAYLWLIGALDWSAQYQRPTIHRLHRQQAEERRDARVA"
     gene            complement(1776..1952)
                     /locus_tag="VFT30_00155"
     CDS             complement(1776..1952)
                     /locus_tag="VFT30_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEU5091063.1"
                     /translation="MLKWAAIFFVIALIAAAFGFTGIAAGAASIAKVLFYLFLGIFVV
                     FLIAGIIVGNKVFS"
BASE COUNT          513 a          597 c          565 g          448 t
ORIGIN      
        1 gattgagggc agcgagtggg gttcgtcacg gaggggtact ttgattcgga gcgattggtt
       61 tcgagcgaat gaaaagaggc gataaaagac ggtaaaggta ccaatcggca acccgtcatg
      121 gttgcgacgc agccgttcat cggtcgcact caaatcgaag agcatcggaa acggacgagg
      181 aatctcagct ctcaaatact ggagtgtttc acgcaagcgg cttcggtcga gccacatcgt
      241 gggaatttca tctctggtcg gctgaaaggc gagcagtccg tctgggccga agcggctcgc
      301 gagttcctct accacccccg aaccatgcac cacctgagcg tcttccatag tcggtgatcg
      361 tagattcatg gcttgggagt tgtcggtcgg tcgagcggtt gtcctgcaaa gtggggtgaa
      421 agcagatcgg ttctgggcag gatatccaca gtcgggtatt cgcggagtcg aacccgctct
      481 tcccgtttgg catcccgcat tgaaggcatg atgggcttct gcacttcctg tgggccgaac
      541 caccagctta aaggccggcg ctctttaccg accttgtctt gcagcaacag caaacattcc
      601 atgaacgcat ggggcggcgg cgggcaaccg ggcatgtaca catcaaccgg aagaaatttg
      661 tcgactccct gtacgaccga gtacgcatcg aacatcccgc cggagtttgc gcaggagccc
      721 atcgagatga cccaccgcgg ttccatcatc tgctcgtaca gttgtttgat gatcggagcg
      781 actttgatga agaccgttcc cgacaccacc agcacatcgg cttggcgcgg agaaccgcgg
      841 acgacttcgg cgccgaatct cgccacgtcg tacacgctgg tgagactcgt cgccatttct
      901 acgtagcagc aggacaggcc aaagttcagc ggccatagcg aattcttcct gccccaagcc
      961 aacaggtctt gcagctttgc gagcattacc gactgaccga cgacggaggt caaggatccg
     1021 tccccaccgt ctcttataga aacgccctca agtcgtccgt tatgccacgc gcgcatcacg
     1081 ccgctcctcg gcctgctgac gatgaagacg atggatagtt ggccgttgat actgtgcgct
     1141 ccagtccaat gcaccgatga gccagagata agcgagggcg gccaagagga tcgtgacaaa
     1201 gatcaccacc tcgataaagc cgagccagcc tgtctccttg acggccaccg cccacgcata
     1261 gagaaacacg gcttccaagt caaaaatcac gaagaacatg gcgaccaggt aatattggat
     1321 gggaaggcgg atttgggcac tgcccgtcgg acggattccg gattcgtatg ggacgccggt
     1381 ggcaatctcc cgtcgccgtt cggactttcg ccaatgtcgt tctcccagga gtgccggtag
     1441 gcccagcatc acgccgatga cgatgaaaac accagccgca tagaggccga tctgaaatat
     1501 ccactgaagt gccatccgag cctctccttg accgcaaggt catgcagggg gatcttccac
     1561 cttgaagtac gttaagcaag tggtgagcca gtatgtcgga tcaatcaagg tagccagaga
     1621 taatcgccag tttcacaaat tgctgagaca ggcatctacg gcggacagct tgtccgaggc
     1681 atagacagct tgtcttgtcc gaataactcg aggtgtcccg ataccagccg cttttttaaa
     1741 cgacgagagc ggtattttct ggaggcaaac ggtaattaag aaaatacttt gttgcccacg
     1801 atgatgccgg cgatcaagaa cacgacaaag atcccaagaa ataaatagaa caagaccttt
     1861 gcaatgctcg ccgctccagc ggcgatgccg gtaaaaccga atgccgctgc tatcaatgcg
     1921 atcacaaaaa agatagcggc ccatttcaac agcatagagt cctctctttt ccagaccgtt
     1981 cactttacat ggcgcaaaat caatgcctga tgcaactcaa gcacgaagcc tccaagtgaa
     2041 gactcgactc gctaatcctt catgttttct tttagtgctt ttgcctcccc ttttcctttt
     2101 tccaccgcgc ctttaacatt gcc
//
LOCUS       DASTWE010000015         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1260766, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000015 DASTWE010000000
VERSION     DASTWE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260766"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFT30_00160"
     CDS             <1..543
                     /locus_tag="VFT30_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5091064.1"
                     /translation="QRDRVMLELLYAAGLRVSELVGLKLSQIDVNLGCVRVVGKGAKE
                     RVVPIGQTASKMLVDYLEQVRPTLLKGRSTRVLFISRRGRGLTRQAFWKLLVQRARRA
                     GISKPISPHMLRHSFATHLLEGGADLRVVQSMLGHADIATTQIYTHVEGSRLKHIHRR
                     YFPRQLSRRRTRAENSIKRS"
     gene            643..718
                     /locus_tag="VFT30_00165"
     tRNA            643..718
                     /locus_tag="VFT30_00165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:676..678,aa:Val,seq:tac)
     gene            850..927
                     /locus_tag="VFT30_00170"
     tRNA            850..927
                     /locus_tag="VFT30_00170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:885..887,aa:Asp,seq:gtc)
     gene            1002..1703
                     /locus_tag="VFT30_00175"
     CDS             1002..1703
                     /locus_tag="VFT30_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU5091065.1"
                     /translation="MFQAGPVGVVLSLGIVSKVVLLLLVCFSITSWAIIFYKLAVFRT
                     ADAKDRHFMTVLLRARDVEEVTRHAQQTVGSPCAKIFHAVIQRIGYIPTEVNENGSVA
                     YDRHVMERTAAHLAQGQIARLESFLPFLATTGNICPFVGLLGTVMGIIDSFREIGTQG
                     TASIAAVAPGVSEALIATAAGLFAAIPAVVAYNYFLVRIRRAAMHMDSVVVELLALIP
                     AQTRPVAPVPVGAKG"
     gene            1700..2143
                     /locus_tag="VFT30_00180"
     CDS             1700..2143
                     /locus_tag="VFT30_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU5091066.1"
                     /translation="MIFETRQRRFLAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIK
                     LPTSASNTIKPEIRAVLTIEKDQRLYLDKDQVSVAQLERKLRVMKEEHADVSLYLRAD
                     RDVPYGIVVQVMDGVKKAGIEKLGMVTDPTGPERVSEGTPSQHNQ"
     gene            2157..>3188
                     /locus_tag="VFT30_00185"
     CDS             2157..>3188
                     /locus_tag="VFT30_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5091067.1"
                     /translation="MQAWASAGMVSDDEWQATLTRRLGFAVIVSLVLHIGILAMVTWV
                     RLPRHSERPLTSIEISLANLPTLPEKAVEPPKSQPIKKEVEPPKPIQQTKPIERSKAI
                     AQPKPIEHPKPVEPLKPLEQPKSVPSVKAAPVSLPPTKAAPVPSVAPAKSSNDLMRDI
                     MKDIELPPDAPKRGDISPEDKPRKSPVIKLPDVPVLTETKETTQKKLIASAQSPSLTE
                     DLSKELDEELKKIKKLEVPKAAPAAEVPSKPAPPVEAKVQNAKAVDTTLKVPGMAQGS
                     NAYLALVRQRISNSWNAPPMDLTSQAYVVVVQFRLHKNGSVTGVTIEQSSGNEYYDLA
                     GKRAVLSANP"
BASE COUNT          750 a          819 c          901 g          718 t
ORIGIN      
        1 caacgcgacc gcgtgatgct ggaattgctg tatgcggcgg gtcttcgtgt gtcggagctt
       61 gtggggctca aactgtcgca aatcgatgta aatctgggct gtgtacgagt ggtggggaaa
      121 ggtgccaagg agcgagtcgt cccgattgga cagaccgcga gtaagatgtt ggtcgattat
      181 ttggaacagg taagaccgac tctactcaaa gggcgatcaa cccgcgtcct gttcatcagc
      241 cggcgaggac ggggactcac gagacaggca ttttggaagc tgcttgtgca gcgggcacga
      301 cgtgccggta tttccaagcc gatttcgccg cacatgctgc ggcattcttt tgccacgcat
      361 ctgctggaag gtggagccga tctgcgagtc gttcaatcca tgttggggca tgctgatatc
      421 gcgacgactc aaatctacac gcacgtggaa gggagccggc taaagcacat ccatcgccga
      481 tatttcccac ggcagctcag tcgacggcgg acacgcgcag aaaattcgat aaagcggtca
      541 tagttaggaa tcgggatatt gcgaaaccgg atgaacagcg cgcaaacccg ttgacaagga
      601 agtatggact tgatacgctc cgcggaggtt gctaagaaaa tcgggcggat agctcagttg
      661 gaagagcgct gcccttacaa ggcagaggtc acaggttcaa tccctgttcc gcctaccagt
      721 cggagctatt ggtacgcaga gacaggaagt gtgcttgccg cgcggaggat ctgttcctac
      781 atgcaggaat tgatcggcga ccaacgattt cgtcttgtcg tgctgctgtt ctgccatcga
      841 tagatttgcg gggccgtcgt tcagcctggt taggacgcca gattgtcaat ctggaggtcg
      901 cgggttcaaa tcccgtcggc cccgccattt tcttcagagt ttggtacaat tccactttgc
      961 caagaaatca tgagcgctga ggaaggggag tagagattcg tatgtttcaa gccggcccag
     1021 tgggagtcgt tctgtcgctc gggatcgtgt ccaaggtggt cctattgctc ttggtctgct
     1081 tttcgatcac gtcatgggcg atcatcttct acaaattggc cgtatttcgc accgctgatg
     1141 caaaagatcg tcattttatg accgtgttgc ttcgggctag agatgtggag gaagttactc
     1201 ggcatgccca acagacggtt gggagtccct gcgcgaagat ttttcatgcc gtgattcaac
     1261 ggatcggcta cattcccacc gaggtgaatg aaaacggctc cgtcgcgtat gatcgacatg
     1321 tgatggagcg gacagcagcc catcttgctc aaggtcaaat cgccaggttg gaatcatttc
     1381 tcccgtttct tgcgacgacc ggaaatatct gtccgttcgt gggcctcttg ggtacggtga
     1441 tgggtattat cgactcattc cgggaaatcg gcacacaggg tacagccagc attgcggctg
     1501 tggctccggg cgtttccgaa gccttgattg cgaccgcagc cggattgttt gccgcgattc
     1561 ccgccgtcgt cgcctataat tattttctcg tacgtatcag gcgtgccgcc atgcacatgg
     1621 attcggtcgt ggtggagctc ctggccttga ttccagccca aacgagacct gtcgctccgg
     1681 ttccagtggg agcgaaggga tgatttttga gacgagacag cgtcgatttt tagcggagat
     1741 caatgtgatt ccgctcgtgg acgttgtgct ggtgctcctg gtgatcttta tggtcacggc
     1801 accgatgctt tatcgaggca tggacatcaa attgccgact tcagcgtcga acacgatcaa
     1861 gccggagatc cgagcagtgc tgacgatcga aaaggatcaa cgcctgtatc tcgacaaaga
     1921 tcaggtgagc gtggctcagc tggagcggaa gttgcgtgtg atgaaagaag agcatgccga
     1981 tgtctcattg tatttgcgcg ccgaccgcga cgtgccatat ggaattgtgg ttcaggtgat
     2041 ggatggagtc aaaaaggccg gtatcgaaaa actcggcatg gtgactgatc ccaccggacc
     2101 tgaacgtgtc agtgagggta cgccatccca acacaaccag taggataagg tccacagtgc
     2161 aggcttgggc atcggccggc atggtctcgg atgatgaatg gcaggcgaca ctgactcgtc
     2221 gcttaggctt tgccgttatc gtctctctgg tgctacatat tggcatactg gcgatggtga
     2281 cctgggttcg actgccacga catagcgaac ggcctctgac gtccattgag atttccctcg
     2341 caaatctacc gactcttccc gagaaggccg ttgaaccgcc gaaaagtcag cctattaaaa
     2401 aagaggtaga accacccaag ccgatacagc agactaagcc tattgagcga tccaaagcca
     2461 ttgcacagcc caagcccatt gagcacccca aacctgttga gccactcaaa cctcttgagc
     2521 agcccaaatc tgttccttcc gtgaaagcgg cacctgtgtc gcttccgcct acgaaggccg
     2581 cacctgttcc ttcagtagct ccggcaaaat cgtcgaatga tctcatgcgc gatatcatga
     2641 aagatattga gttgcctccg gatgccccga agcgtggcga catcagtccg gaggacaagc
     2701 caaggaaatc gccggtgatc aagctgcctg atgtaccggt ccttacggaa accaaggaaa
     2761 caacgcaaaa aaagctgatt gcctccgctc aatccccctc cctgacggaa gatttgtcga
     2821 aggaattgga tgaggaactg aagaaaatca aaaaacttga ggtgcccaaa gcggcacccg
     2881 cggcggaagt accgtcaaaa cctgctcccc cagttgaagc aaaggtccag aacgccaagg
     2941 ctgtcgacac cacattgaaa gtgccaggaa tggctcaggg atccaatgcg tatcttgcgc
     3001 ttgtgcgaca gagaatcagt aattcttgga atgccccgcc catggatctg accagtcaag
     3061 cctacgtcgt tgtcgtacag ttcagacttc ataagaacgg atccgtcacc ggtgtgacga
     3121 tcgagcaatc ttcaggaaac gagtactatg atttagccgg gaaacgagca gttctcagtg
     3181 caaaccca
//
LOCUS       DASTWE010000016         1352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_129712, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000016 DASTWE010000000
VERSION     DASTWE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129712"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..467)
                     /locus_tag="VFT30_00190"
     CDS             complement(162..467)
                     /locus_tag="VFT30_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5091068.1"
                     /translation="MVLDEGRVDMTKAQIIEKVSEQVTTLTKRQAEVVVNTIFDCVKD
                     SLKNGDKTEIRGFGSFRLRARRMKEGRNPKTGETVAVPAKRVPFFKAGKELKELLNQ"
     gene            complement(487..>1352)
                     /gene="sppA"
                     /locus_tag="VFT30_00195"
     CDS             complement(487..>1352)
                     /gene="sppA"
                     /locus_tag="VFT30_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEU5091069.1"
                     /translation="RHLLWKAFWLFAVGLGVLILVNLFFPDLDLSTDDRIALIRVEGV
                     ILDSQTTIGELKRFSENPSVKAIVIRIDSPGGGVVPSQEIHDAVKRIRSKNNKAVIAS
                     MGSVAASGGYYIAAATDRIVANPGTLTGSIGVIMETANVEGLLQKIGVEGVVIKSGKY
                     KDVGSPLRKMSADERGLLQAVMDDVHKQFIEAVAEGRSLELRAAQALADGRIFTGRQA
                     KEAKLVDELGDLEDAIQLAAEVVGIEGEPKVIEPRRRFSLREILDSRLSMMLPKLDMQ
                     PGVSLKYLMAF"
BASE COUNT          301 a          409 c          302 g          340 t
ORIGIN      
        1 caattcacgc gctttctttt cggccaaacg acggaccata ctgcgcagaa atatcggtgc
       61 tcgctccatt cgtttgagcg cgccgtcggt ccaacggatt tctgttgtat tcgtctgcga
      121 agaatctgat gccgacattg gttccttttt caaccggatc gctattgatt gagcaattcc
      181 ttcagctctt taccggcttt gaaaaacgga acccgcttgg ccggtaccgc aactgtctct
      241 ccagtctttg gattccgccc ttccttcatc cgacgagccc gcagccgaaa gctgccgaag
      301 cctcgaatct cagttttatc gccgtttttc agcgaatctt tgacacaatc gaaaatggtg
      361 ttgacgacca cttctgcttg tcgcttcgtc aaagtggtga cttgctcaga aactttttcg
      421 atgatctgcg ccttggtcat atccactctc ccttcatcga gaaccaccac atcttcattg
      481 acacagctag aaggccataa gatatttcaa gctcacaccc ggttgcatat ccagcttcgg
      541 aagcatcata ctgagtctcg agtccagaat ttctcgaagg gaaaatcgac ggcgcggctc
      601 aatgacttta ggttcccctt caatccctac cacctctgca gccaattgaa tggcatcttc
      661 caagtcgccg agttcgtcga ccagcttcgc ttccttggcc tggcgtccgg taaaaattcg
      721 accgtcggct aacgcttggg cagcacgaag ctcgagcgaa cggccttccg ccaccgcttc
      781 aataaactgc ttgtggacgt catccatcac ggcttgcaac aaacctcttt catccgcact
      841 catcttacgg agtggagaac ccacatcctt atatttcccg ctcttaatga cgaccccttc
      901 cacaccgatc ttctggagca acccctcgac gttggccgtt tccatgatga ctccgatgct
      961 accggtcaga gttccaggat tggccacgat ccgatccgtt gcggcagcga tgtaataccc
     1021 tcccgatgcg gcgacgctcc ccatcgaagc gatcaccgcc ttgttgttct tgcttctgat
     1081 ccgcttgacg gcgtcgtgaa tctcttgcga tggcacgacg ccgcccccgg gactatcgat
     1141 tcttatgacg atggccttga cagaaggatt ctcgctgaat cgcttcagct ctccgatggt
     1201 cgtctgagaa tccaaaatga ctccttcgac tcgaatcaga gcgattcggt cgtcggtcga
     1261 cagatcaaga tcgggaaaaa agagattgac gagaatcaag acaccgagcc ccactgcgaa
     1321 aagccaaaag gctttccaca gcagatggcg ct
//
LOCUS       DASTWE010000017         1244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_388243, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000017 DASTWE010000000
VERSION     DASTWE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388243"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VFT30_00200"
     CDS             <1..1144
                     /locus_tag="VFT30_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU5091070.1"
                     /translation="GPIGTKGSRVTTYVSLPGRYLVFMPNVDHIGVSRRIPRDEERAR
                     LKEIMRRVRRSGFGYIVRTVSEGVKEDELKSDVDFLHVLWQDILTKREQLPAPALLHS
                     DLSLSFRVVRDLFGKKVDRLWIDSREEYEAIRDFVQRFSPEQTSRIHFYDKDEPLFDH
                     LGVEQEIARALSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETILRNNLEA
                     AKEVAYQLKLRGIGGIIIVDFIDMEREKNREKVYHALVDAMASDKARTRISRISDLGL
                     IEISRERVREDLLRSLSEPCRYCEGRGYTKSPTTVAYEIFREIRRIEPSADQQRIIVG
                     AHPTVAELLQDEERHGVEVLERDCSAKIIVTPDSQLHLEQYDLVVF"
BASE COUNT          283 a          297 c          389 g          275 t
ORIGIN      
        1 aggcccgatc ggcaccaagg ggtcgcgagt gacgacgtat gtctcacttc cggggcgata
       61 cctcgtcttc atgcctaatg tcgaccatat cggcgtgtct cggcggattc cgcgggatga
      121 agaacgggcg agactgaagg agatcatgcg ccgggtcagg cgctccggct tcggctatat
      181 cgtgcggacg gtcagcgaag gcgtcaagga agatgaacta aaatccgacg tcgactttct
      241 gcacgtcctc tggcaggaca ttttgacgaa gagggaacaa ctgcccgctc ccgcgttgct
      301 gcattctgat ttgagcttga gcttccgtgt cgtgagggac ctgttcggca aaaaggtgga
      361 tcggctgtgg atcgattcgc gagaggaata cgaagctatt cgggatttcg tacagcgatt
      421 ttctcccgaa cagacctcgc ggattcattt ctacgataag gatgagccgt tgttcgatca
      481 tctgggggtg gagcaggaga ttgcgcgcgc attgagccgg aaagtttggc tcaagtcggg
      541 gggctatctc gtgatcgatc ataccgaagc aatgacggtg atcgacgtca ataccggacg
      601 cttcgtggga aaacgggatc aggaagagac cattttacgc aataacctcg aggcggccaa
      661 agaggtcgcc tatcaactga agctgcgggg aatcggtggc atcatcattg tcgacttcat
      721 cgacatggag cgggagaaga atcgggaaaa ggtctatcat gcgttggtgg acgccatggc
      781 gtcggacaag gcgagaacga ggatatccag gatttcggat ctcgggttga tcgaaatctc
      841 ccgcgaacgg gtgcgggagg atctgttacg gtcgctgtcg gagccgtgcc gctactgcga
      901 aggccgtgga tatacgaagt ctcccacgac cgttgcgtat gagattttcc gcgaaatcag
      961 acggattgaa ccgtctgccg atcagcaacg gattatcgtg ggagctcatc cgacggtggc
     1021 tgaattgctg caagacgaag agcggcacgg agtggaagta ttggagcggg actgttcagc
     1081 gaaaatcatt gtcacgccgg acagtcagtt gcatcttgag cagtacgacc tggtcgtgtt
     1141 ctagtcgagt accttcacac cgccgatttt tgttaaggag atattctgcc tgccggactc
     1201 tcctgctgtg gaaatgaatt ccctgccgat gatcgagagt gatc
//
LOCUS       DASTWE010000018         1578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_65145, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000018 DASTWE010000000
VERSION     DASTWE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65145"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /gene="tmk"
                     /locus_tag="VFT30_00205"
     CDS             complement(<1..419)
                     /gene="tmk"
                     /locus_tag="VFT30_00205"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5091071.1"
                     /translation="MARTPRKPTGIFITLEGGEGSGKTTHALLLYQWLTAQGLNVLQT
                     REPGGTPLAEQLRTILLDSPAESIASETEALLILAARRQHVDHVIKPALAQGKTVVCD
                     RFSDSTMAYQGYGRRLDQRALRAMNDWATGKLVPHLTL"
     gene            623..1291
                     /locus_tag="VFT30_00210"
     CDS             623..1291
                     /locus_tag="VFT30_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEU5091072.1"
                     /translation="MLKTALRRYFLTGLLLVTPIWGTILVLKTLFVAVDGILGDAVAE
                     LVPDHYIPGLGILTLVLLIFLVGLFAANFIGRQIVGHWEDWLNRLPLVRGIYSTLKSM
                     MDILSFSERGSYRRVVLIQFPKNGHYCFAFVTGMTKGETTALGQEALIHVYVPTSPNP
                     TSGYFLLVPEREVTSVDISIEEAMKLIVSGGLYTPSGAMTSALSGEAKWSQVKQPDAG
                     VPIG"
     gene            1341..>1578
                     /locus_tag="VFT30_00215"
     CDS             1341..>1578
                     /locus_tag="VFT30_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5091073.1"
                     /translation="MKLSVDPHTTASHSPGLGVIVMAAGLGKRMKSDQVKVLHHVAGQ
                     PMVLYAVDVALQLAGHRIAVVVGHQSDRVRHVIET"
BASE COUNT          337 a          399 c          446 g          396 t
ORIGIN      
        1 agggtgagat gcgggaccag cttccctgtg gcccaatcat tcattgcgcg taacgctcgt
       61 tgatccaatc tccgtccata cccttgatag gccatcgtcg aatctgagaa ccgatcgcag
      121 acgaccgtct ttccttgtgc gagtgcaggc ttgatgacgt gatccacatg ttggcgtctg
      181 gctgcaagaa tgagcaaggc ttccgtttct gaagcaatgg attcagcagg actatcaaga
      241 agaatagtgc ggagctgttc agcaagagga gtgcctcctg gttctcttgt ctgaagcacg
      301 ttcaggcctt gtgcagtcag ccattgatag agcaggaggg catgcgtagt tttcccactt
      361 ccctcaccgc cttccagcgt gatgaagatg cccgtcggtt ttctaggtgt tctagccatc
      421 ggcgattttc atttcagccg taattcgtgg agcgatccta tgtcaggttg cggtgaatag
      481 caagaccgat accgtccagg catttcatca agactaagca cataacccgt tgaaatttac
      541 aataaaacgc ttgcggggtc tgtccggaac tctgtaagat gaaccactga atattctctc
      601 tgtggctggg ggccggtgat tcatgctcaa gaccgcgcta agacggtatt ttctgacagg
      661 actccttctt gtcactccta tctggggcac tattctcgtc ctgaagaccc tgttcgtcgc
      721 agtagacggc atcttaggcg acgccgtcgc agaattggtg ccggaccatt atattcccgg
      781 cctagggatc ctcactctgg ttctgctcat ctttttggtg gggttgtttg cggcgaactt
      841 catcgggcgt cagattgtgg gacactggga ggattggctt aaccgactgc ccctcgtacg
      901 gggaatttat tccacgttga aatccatgat ggatatcctg tccttttcgg aacgaggatc
      961 gtatcggcgt gttgtcttga tccaattccc gaagaacggc cattattgtt ttgcgttcgt
     1021 gaccggcatg acgaaaggcg agacaacggc actgggccag gaagcgctga ttcatgtcta
     1081 tgtgcccacc tcgcccaatc cgacgtcggg ctattttcta ttggtgccgg aacgggaagt
     1141 gacatctgtt gacatcagta ttgaggaagc gatgaaactg attgtgtcgg gtggtctcta
     1201 tacgccgtcc ggcgcaatga cctcggccct gagcggggaa gcgaagtgga gtcaggtcaa
     1261 acagccggat gccggtgtgc cgatcggata aatacggtgt cctatactcg attgagagtc
     1321 tctcatatcc ggagccctcg atgaaactat cggtagatcc acacacaacg gcgtcgcata
     1381 gtccaggatt aggggtaatc gtgatggcgg ccgggttggg caagcggatg aagtccgacc
     1441 aggtaaaagt gctgcaccat gtcgctgggc agccgatggt gctctacgcc gttgatgtgg
     1501 ccctccaact ggcgggccat cgcattgcgg tcgtggtcgg gcatcaatcc gacagagttc
     1561 gccacgtgat tgaaacag
//
LOCUS       DASTWE010000019         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1519404, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000019 DASTWE010000000
VERSION     DASTWE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519404"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="tgt"
                     /locus_tag="VFT30_00220"
     CDS             complement(<1..855)
                     /gene="tgt"
                     /locus_tag="VFT30_00220"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5091074.1"
                     /translation="MMQYQVEQSDRYSKGRTGLLTTGHAEVNTPAFMPVGSLGPVKGL
                     EPEDLQSLGFRLILNNAYHLYLRPGHKIVADMGGLHAFTGWPGAILTDSGGFQIFSLA
                     KLCEVTDEGVTFQSHIDGSTHFITPEKAIEIEEALGADIIMVLDQCKALPAGREIIRE
                     SVRRTQLWAERCQASRRRTDQALFGIVQGGLEADLRVASARELVRLGFDGYAIGGLSV
                     GEDKSDMYAMLDVTVPELPESKPRYLMGVGHPEDLLEGVARGIDLFDCVVPSRHGRTG
                     SLFTTTGRV"
     gene            complement(875..>2144)
                     /gene="argS"
                     /locus_tag="VFT30_00225"
     CDS             complement(875..>2144)
                     /gene="argS"
                     /locus_tag="VFT30_00225"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5091075.1"
                     /translation="AVVRLLNAIGYDAVGEYYINDAGRQMKLLGASVYARYRELSGQT
                     VDFPEDGYHGSYITSVAQQLKEQLDRVAGELTPADLETRCRSLAYQKLLGLIRDDLRS
                     FGIEIQSWFSETSLLESKAIERALDELKARELLFQQEGAWWFRASMYGDEKDRVVKKQ
                     DGEYTYLASDIAYHHDKLRRGYDLLIDVFGADHHGYIPRMQAVMQAYGHPKDRLHVVL
                     VQLVKLLRDGVEVKMSKRTGEFITMREVIDEVGADAAKFYFLMRDSKTHLDFDLDLAK
                     QRSADNPVYYVQYAHARICSLWCVASERGIARPSASETDLTVLTDPDELGLIKKLSAY
                     PEMIQASALAFEPHRMTYYLQQLAAQLHTFYNKHRVLPPVTDQEHDDSASAEVLTPKR
                     TAARLVLMGAVQHVLRNGLEVLGISAPEHM"
BASE COUNT          474 a          644 c          552 g          474 t
ORIGIN      
        1 gacccgaccg gttgtcgtaa acagcgagcc tgttctgcca tgacgcgagg gaacgacgca
       61 atcgaagaga tcgatgccgc gcgccacccc ttcgagcagg tcttccggat gtccgacccc
      121 catgagatac cgaggcttgc tctccggcaa ctctggaacc gtcacatcga gcatcgcata
      181 catgtcggac ttatcttctc cgaccgacag tccgccgatc gcatagccat cgaaccccaa
      241 tctcactaac tccctcgccg aggctactcg caagtccgct tccaatcccc cctgtacgat
      301 gccaaacaag gcctgatccg ttcgtcgcct gctcgcctga cagcgctcgg cccagagctg
      361 agttcggcgc acgctttccc ggatgatctc tcgaccggca ggcaacgcct tgcattgatc
      421 gagcaccatg atgatatcgg ctcccagcgc ttcttcgatt tctatcgcct tttcaggcgt
      481 aatgaagtgg gtcgacccat cgatgtgcga ttgaaacgtg acgccctcat cggtgacctc
      541 gcacaacttc gctaaactga aaatctgaaa cccaccactg tcggtgagaa tcgctcccgg
      601 ccatccggtg aaggcatgca gccctcccat gtcagccaca atcttatgac cgggtcgcaa
      661 gtatagatga taggcattat tgaggatgag tcgaaacccg aggctctgca gatcctccgg
      721 ctcgagcccc ttgactggtc ccaacgagcc taccggcatg aaggccggtg tgttgacctc
      781 tgcatgaccg gtcgtgagca ggccggtgcg gcctttggaa taccggtctg attgttcgac
      841 ttggtactgc atcatcttct atggggtcca accgctacat gtgctcaggc gctgagatgc
      901 cgaggacctc aagtccgttt ctgagaacgt gctgaaccgc ccccatcaag accagtcgcg
      961 ccgcagtccg tttcggcgtg aggacttcgg cggacgccga gtcgtcatgc tcttgatcgg
     1021 tcacgggagg caacacacgg tgtttgttat aaaacgtatg cagctgcgcc gccaattgct
     1081 ggagataata cgtcattcga tgcggctcaa aagctaacgc gctggcttgg atcatttcag
     1141 ggtaggcgga aagttttttg atcagcccca attcatcggg atcagtcaac acagtcaggt
     1201 ccgtctccga tgcggacgga cgggcgattc ccctttcgga agccacacac cagaggctgc
     1261 agatcctcgc atgtgcatat tggacgtagt acaccggatt gtcggcggac cgttgcttcg
     1321 ctaaatccag atcgaaatcc agatgagtct tggagtcccg catcaagaaa tagaacttcg
     1381 cggcgtcggc tccgacctca tcgatgactt cccgcattgt aatgaattcc ccggtccgct
     1441 tggacatctt cacttcgact ccgtctcgca acagcttcac caactggacc aggacgacat
     1501 ggagacgatc ttttggatgt ccataggcct gcatgacggc ttgcatgcgt gggatatacc
     1561 catggtggtc agcaccgaag acatcgatca acagatcgta gccacgcctc agcttgtcat
     1621 ggtgataggc gatatcggac gctaagtacg tatactcacc gtcctgcttc ttgacgacac
     1681 ggtctttttc gtcgccgtac atggacgccc gaaaccacca cgcaccttcc tgttggaaca
     1741 agagttcgcg ggctttcaat tcatccaaag cccgctcgat ggctttggac tcgaggagcg
     1801 aagtttcgct gaaccaggat tggatctcga taccaaatga ccggaggtcg tcacggataa
     1861 gtcctagcag tttctgatag gcaagagatc ggcagcgagt ctcaaggtcg gcaggagtca
     1921 gttccccagc cacgcgatcg agttgctcct tgagctgttg tgccacagac gtgatgtacg
     1981 aaccatgata accatcctct gggaaatcga cagtctgccc cgacaactct cggtaacggg
     2041 catacacaga cgcccccaac aatttcatct gtcgtccagc gtcgttgata tagtactcgc
     2101 ctacggcatc gtatccgatc gcattcagta atcggacaac tgcg
//
LOCUS       DASTWE010000020         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1487075, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000020 DASTWE010000000
VERSION     DASTWE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487075"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFT30_00230"
     CDS             <1..407
                     /locus_tag="VFT30_00230"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5091076.1"
                     /translation="LGILYTNLALQEARASQWDQAVASFTQALDHHRAVGNEEGLAVT
                     YSQLGKCYLDQGDLTRAERCLNNASEHYIKLGNEPAEAAVLRLLAQVYETRQDTVSAL
                     RCLMRVVALDERYALPELQADSAHLSRLSPSR"
     gene            541..1143
                     /locus_tag="VFT30_00235"
     CDS             541..1143
                     /locus_tag="VFT30_00235"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU5091077.1"
                     /translation="MASLARRFSVLVVGTMMTASFVHAENISPTITVGTQEVGLTAGY
                     LFSHRLTDLHTTKQHGPALMPSWMMTITDPIGDSWYRGQVSLGAEMLYLEFREPLLTH
                     GIGFNPRIKYTFVALGRIRPYLEFAGGPFWTDLGGRIREQANEFIFVLTGGVGISWFL
                     TPQLAFNAGYRFHHISNAGTAFPNLGLNASLPFAGFSFYF"
     gene            1157..>1406
                     /locus_tag="VFT30_00240"
     CDS             1157..>1406
                     /locus_tag="VFT30_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091078.1"
                     /translation="MTMNMLRWSVLSLAGFVLCSITGCTRWIDVKPPSVDPKETISHS
                     LPTDERVPLVTNSFHLSRNGASQYPSIDLERRILSSVQE"
BASE COUNT          302 a          416 c          358 g          330 t
ORIGIN      
        1 gacttgggat tctttatacc aacctcgcgc tgcaagaggc ccgcgctagt caatgggatc
       61 aggccgtcgc ttcattcaca caagcgctcg atcatcatcg ggcggtcggc aacgaggaag
      121 gacttgccgt cacctatagc caactcggca aatgttacct cgatcaagga gacctcacta
      181 gagccgaacg ctgcttgaac aacgcctcgg aacactacat caagctcggc aacgaaccgg
      241 ctgaggccgc ggtgctccgt cttctcgcgc aggtttacga gactcgccag gataccgtct
      301 ccgctctgcg atgcctcatg cgcgtggtgg cgctcgacga acgctatgcg ttgccggagc
      361 ttcaagccga ctccgcccac ctctctagac tcagtccatc cagataatcc ttttcgacct
      421 agtactcctt cgcttctgga cagaatccga tcctctgcta cctttgaagc agcacacctg
      481 tacgcctgtt gatcaagttt cacctcacag aagccgacag gatattgacg tggagatagt
      541 atggcttcgc tcgctcgacg gttcagtgtc ctggtagtcg gaacgatgat gacggcgtcc
      601 ttcgtccacg ccgaaaacat ctctcccacg atcacggtcg gcacccagga agtcggactt
      661 accgccgggt atttgttttc tcaccgacta acagatcttc acacgaccaa acaacatgga
      721 ccggcgttga tgccgtcgtg gatgatgacc atcaccgatc ctatcggcga cagctggtac
      781 cgaggacagg tttcgctcgg cgcggagatg ctctacctcg agttccgaga acctctgctc
      841 acgcatggca tcggattcaa cccaagaatc aaatatacgt tcgttgcctt gggtcgtatc
      901 cgcccatacc tggaattcgc gggcgggccg ttctggacgg atctcggtgg tcgcattcgt
      961 gagcaagcca atgaattcat cttcgtcctc acgggaggcg tgggcatatc gtggtttctt
     1021 acgccgcaac tcgccttcaa tgcgggctat cgttttcacc acatttccaa tgccggcacg
     1081 gcctttccga atcttgggct gaatgcgagt ttgccctttg caggattttc attctatttc
     1141 tgaggagacg gcgatcatga caatgaacat gttgcgatgg tcggtcctga gtcttgccgg
     1201 gttcgtgctg tgcagcataa cgggatgtac cagatggatt gatgtcaaac cgccctcagt
     1261 cgatcccaaa gagaccattt cgcattcgct ccccacggat gagcgggttc cgctcgtgac
     1321 gaattcgttt cacctctcaa gaaacggagc gtcgcaatat ccctctattg atctcgagcg
     1381 acggatctta agcagtgtcc aagaaa
//
LOCUS       DASTWE010000021         1361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_259102, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000021 DASTWE010000000
VERSION     DASTWE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259102"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..>1361
                     /gene="sthA"
                     /locus_tag="VFT30_00245"
     CDS             117..>1361
                     /gene="sthA"
                     /locus_tag="VFT30_00245"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEU5091079.1"
                     /translation="MAHYDLLVIGTGPAGQKAAIQAAKLNKKVGIIERKTVVGGVCIN
                     TGTIPSKSLREAVLYLSGFRQRNIYGGEYRLKKKITIEDLAFRANHVIKHEIQTVQDQ
                     MARNQVDMFFGSADFLDPHRLRIQRDDGSLELTSDFIIIAVGTEPARPPDVPFDDKTI
                     IDTDGLLTLRRIPDSIVIVGGGVIGTEYASILAALGIPVTLIDKRPRLLEFVDVEIVE
                     TLQQRMKDLGVILYHDEEVLAIKKTWTKSIHVSLRHNRPVQTSTLMYATGRVGATQSL
                     NLQTIALKPDERGRLPVNEHFQTMIPHIYAVGDVVGFPALASTSMQQGRHAACHAFGH
                     PDRTDTSLLPYGIYAIPEISMVGRNEEELKQAEVPYGVGIARYREIARGQLIGDETGM
                     LKLLFHRHTRHLLGVHCIGEGAT"
BASE COUNT          326 a          397 c          357 g          281 t
ORIGIN      
        1 ttgctcgtcg gttggtgaca gagagttaag aggagatact ttggacctga ggtgacggga
       61 gagcacgtaa ccgtttgcta ggactcaacc gtaacctcgg tgctccagag gctctgatgg
      121 ctcattacga tctactggtc atcggcacgg gaccggccgg ccaaaaggct gctattcagg
      181 cggccaagct caacaagaag gtcgggatca tcgagcgaaa gacggtcgtc ggtggggtct
      241 gcatcaatac cggcaccatt ccaagcaaat ccctccgcga agcggtgctc tatctctccg
      301 gatttcgcca gcgcaatatc tacggaggcg agtaccggtt aaaaaagaag atcacgatcg
      361 aagaccttgc cttccgcgcg aaccatgtca tcaagcacga gatccagacc gtgcaggatc
      421 agatggctcg gaatcaggtc gatatgtttt tcggttcagc cgactttctc gatccacatc
      481 ggctacgcat tcaaagggat gacggatcgc ttgagctgac gtcggatttc attatcatcg
      541 ccgtagggac agaaccagcc agacctccgg acgtcccgtt cgacgataaa accattatcg
      601 atacggacgg tcttctcact ctgagacgca tcccggactc cattgtcatc gtcggaggcg
      661 gtgtgattgg aacagaatac gcgtccattc tcgccgcatt gggaatcccc gtcaccctca
      721 tcgataaacg gccgcggctg ttggagttcg tcgacgtaga gatcgttgaa acacttcaac
      781 aacgcatgaa ggaccttgga gtcatcctgt atcacgatga agaagtcctc gcgatcaaga
      841 aaacatggac caaatccatc catgtgtctc ttcgccacaa ccgtccggtt caaacctcga
      901 cactcatgta tgcgactggg cgcgtcggcg cgacgcaatc gctgaatctc caaaccatcg
      961 cactgaaacc tgatgagcgc ggacgcttgc ccgtcaacga acactttcaa acgatgattc
     1021 cccacatcta tgcggtcggc gacgtcgtcg gctttccggc cctcgcatcg acgtcaatgc
     1081 aacagggccg ccacgccgcc tgccacgcct tcggccatcc agaccgtacc gacacctcgc
     1141 tccttcccta cggcatctat gccattccgg aaatctccat ggtgggtcgg aatgaggagg
     1201 aactcaaaca ggcagaggtg ccttatggag tcgggatcgc ccgctacaga gaaattgccc
     1261 gtggacaact cattggcgat gaaaccggta tgttgaagct actatttcat cgacacacca
     1321 ggcatctgct cggcgtgcac tgcatcggcg aaggagccac g
//
LOCUS       DASTWE010000022         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_485316, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000022 DASTWE010000000
VERSION     DASTWE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485316"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..>1249)
                     /locus_tag="VFT30_00250"
     CDS             complement(11..>1249)
                     /locus_tag="VFT30_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091080.1"
                     /translation="SPALADGLIDLYGLMDKVAGVRIQNDIGEMTALLQAQAGTGDDI
                     RAWADGALAPRLEAIDAVKALADLLTLAAGPGQARIDALLSGLQVVLTYLGDKKAFKD
                     ACKHLSYINEKESFEDIIKAFGATWKKVKPVERKGSLVVSFPPALHISNGQRDSLCFA
                     AELRKIERSISSRDAILVIDEVFDYLDDANLVAVQYYITRLIKKVREKQRSMYPLILT
                     HLNPYYFRNFTFASQKIYFLKTSKPAINKDFRALIAKREDPSIKKDVGRHYLHYDPSP
                     VDVKAQFQALGLREAWGDSIAFQAYTEAEWIKYVNESDNYDPFAVCCFVRVRIEKIVH
                     DAIQDPVRKSEFLDTSKTKNKLDFAVANGVDVDDTIYLLGIIYNEGMHVHDNVDDSSP
                     IVSKLENRTIRHMLIESIRG"
BASE COUNT          266 a          341 c          342 g          300 t
ORIGIN      
        1 tgagagccgg ttatcccctt atgctctcga tgagcatatg gcggatggtc cgattctcta
       61 gcttggaaac gatggggcta ctgtcatcca cgttgtcgtg aacgtgcatg ccctcgttgt
      121 agatgatgcc cagcagatag atggtgtcgt caacgtcgac accatttgcg acggcaaaat
      181 ccagcttgtt ttttgtcttc gacgtatcga ggaactcaga ctttcgaacc gggtcctgga
      241 ttgcgtcatg gacaattttc tcaatccgga cacggacaaa gcagcagacc gcaaaggggt
      301 catagttatc cgattcgttg acgtacttaa tccactccgc ttctgtgtac gcttggaatg
      361 cgatcgaatc tccccaggcc tccctcaagc cgagggcttg gaactgcgcc tttacgtcaa
      421 ccggtgacgg atcatagtgc agatagtggc ggccgacgtc tttcttaatg gatggatctt
      481 cccgctttgc gatgagggcg cgaaagtcct tgttgatggc cggcttggac gtcttcagaa
      541 aatagatctt ctggctagcg aatgtgaagt tcctaaagta gtacggattt agatgcgtaa
      601 gaatcagagg gtacatgctc ctctgtttct cacggacttt cttgatgagc ctagtgatgt
      661 agtactggac agccacgaga ttcgcgtcat ctaggtaatc aaacacctcg tcaatgacga
      721 ggatggcgtc gcgcgacgag atggaccgct caatctttct caactctgcc gcaaagcaca
      781 gcgagtcgcg ctgaccattc gagatgtgca aggctggtgg aaacgagaca acgagacttc
      841 ccttgcgctc aacaggcttg accttcttcc atgtagcacc gaacgccttg atgatgtcct
      901 caaagctctc cttttcgttg atgtaggaaa gatgcttgca cgcgtccttg aacgcctttt
      961 tgtccccaag gtaggtcagc actacctgca ggcccgagag cagcgcatcg atgcgggcct
     1021 gcccggggcc ggcagccaat gtcaggagat ccgccagcgc cttgacggcg tcgatcgctt
     1081 caaggcgtgg cgcgagggca ccgtcagccc atgcgcgtat gtcgtcgccg gttccggctt
     1141 gcgcctgaag caacgcggtc atctcaccga tgtcattttg gatgcggacg cccgccactt
     1201 tgtccatcag gccgtagaga tcaatcagac cgtccgccag cgccgggct
//
LOCUS       DASTWE010000023         1177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1422579, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000023 DASTWE010000000
VERSION     DASTWE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422579"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFT30_00255"
     CDS             <1..547
                     /locus_tag="VFT30_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091081.1"
                     /translation="SAYCAAKHAIQGFTESLRSELIHDNSRVQLSIVQLPAVNTPQFS
                     WIKTRMPRHPQPVPPIFQPEVIARYVYWIAHHPRREFTIGLSAAQAIIGDKFIPGLLD
                     HYLASMGYDAQQTTEPVDPDRPNNLYDPLPGDYGARGRFDDRSTDYSVQAWANRYRGW
                     LTLGLSLAALSSLYLTARRSA"
     gene            544..1002
                     /locus_tag="VFT30_00260"
     CDS             544..1002
                     /locus_tag="VFT30_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091082.1"
                     /translation="MKTTTAFRFLILVIPIVWVTVGLIPAEQHSHGKVHLVASPTQEP
                     SRQQTPGRIAPMTDREKEALDKFVKHELRFKDHYQAHFSTSHYEYNQYRPAYQHGFEL
                     SLDPRYRKMDWSNVEPEARRTWDESAMGLWSQYKDAVRYGWEQGVALERG"
BASE COUNT          273 a          342 c          327 g          235 t
ORIGIN      
        1 atccgcttat tgcgcggcga aacacgcgat tcaggggttc acggaatcgc tgcgttccga
       61 gctcattcac gacaatagtc gagtccagct gtcgattgtc caattgccgg cggtcaatac
      121 gccgcaattc tcgtggatca agacccggat gccgcggcac cctcagccgg tgccaccgat
      181 ctttcagccg gaagtgatcg ccagatacgt gtactggatc gcccatcacc cccgccgcga
      241 attcacgata ggcctgtccg ctgcacaagc gattatcggc gataaattca ttcccggact
      301 cttggaccac tatttggcaa gcatgggata tgacgctcaa cagacaacgg agccggtgga
      361 tcccgatcgt ccgaacaatc tatacgatcc cttgccgggt gactacggcg caagaggccg
      421 gttcgacgac cgttccaccg actacagtgt acaggcctgg gctaatcgct accgcggttg
      481 gttgacgcta gggctcagtc tcgcggcctt atcttctctc tatctgacgg ccaggaggtc
      541 agcatgaaaa ccacgactgc ctttcgattc ttgatcctcg tgatcccgat tgtctgggtg
      601 accgtgggcc tgataccggc agagcaacac agccacggca aggtgcatct cgtcgcatcg
      661 ccaacacaag agccgtcgcg tcaacagaca ccgggtcgta tcgcccccat gaccgatcgc
      721 gaaaaagaag ccctcgacaa gttcgtgaaa cacgagttgc gattcaagga ccattatcaa
      781 gcgcactttt cgacatcgca ttatgagtac aatcaatacc ggccggccta ccaacatggc
      841 tttgagctga gccttgaccc gcgttaccgg aaaatggact ggagcaacgt cgaaccggaa
      901 gcacgccgaa catgggacga aagtgcgatg ggactctgga gtcagtacaa ggatgccgtc
      961 cgctacggat gggaacaggg cgtcgcgttg gaacggggat gacaacaacg ggatgggaca
     1021 tccgaagccg cagttatgat atcggacctg atcgccgctc gatcccagat ggccatgtct
     1081 ctgggttttc acatcgtctt tgccgcgctg ggcatcgcga tgccggcatt gatggccgct
     1141 gccgagtggc gatggctgaa aacgaagaat gaagaat
//
LOCUS       DASTWE010000024         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_259145, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000024 DASTWE010000000
VERSION     DASTWE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259145"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /gene="dnaG"
                     /locus_tag="VFT30_00265"
     CDS             complement(<1..1430)
                     /gene="dnaG"
                     /locus_tag="VFT30_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5091083.1"
                     /translation="MGRSLISDDIKNRIRDRVDIADVVGRHISLRRAGQNLVGLCPFH
                     QEKSPSFSVSPSKQMFYCFGCKTGGDVFAFLSKITGATFPEVLRELGDTVGIAVEESP
                     AERVQRGQTSRIEEINRASMTWFQANLRDLQIGATAREYLNKRGIQQSTVDAFQIGVS
                     SPEWDGLCKFLSRNGFSRSEIMAAGLGSPRQNGNGCYDKFHGRLMFTIADLRKRVVGF
                     GGRVLDDRMPKYLNSPDTLLFKKGQTLFAFDQAREAIVRTKTVIVVEGYFDAIALHQA
                     GLTHTVATLGTALTAEHIQALRRFADQVVLLFDPDAAGVRAALRGLDLFVNSGLGVKV
                     VTLPVGEDPDTFVRKEGAAAFAQLEAAAPSLLDYALNHTVKQAVDGSLESRIRSVDEV
                     LRILQKSEHPIVRQERIKIVAERLGISEARLIERYPALLDQPKRGAEAPRTQPEQGIP
                     LNALFKGLPEERDLLLLLLHGKLSSA"
     gene            complement(1553..1897)
                     /locus_tag="VFT30_00270"
     CDS             complement(1553..1897)
                     /locus_tag="VFT30_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEU5091084.1"
                     /translation="MSECLFCKIVEKKIPAKLVQEDEHTLAFDDINPQAPVHTLVIPK
                     RHISAVQDLGMGDDSLLARLLISCTKVAALKGVRESGYRLVANTGRDAGQTVFHLHFH
                     VLGGRHMAWPPG"
     gene            complement(1963..>2345)
                     /locus_tag="VFT30_00275"
     CDS             complement(1963..>2345)
                     /locus_tag="VFT30_00275"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEU5091085.1"
                     /translation="TGERACFFSRIDEQGRVVHRKAQDAQGGILERILQTIRERRSSP
                     QTGSYASKLFEGGHDKILKKVAEEAGEVLLASKGGKKEEIVYEVADLFFHTLMVLGYH
                     DLSLQDIYEELGSRFGKSGLRSGK"
BASE COUNT          536 a          715 c          564 g          530 t
ORIGIN      
        1 tcggcagacg acaattttcc gtgcagcaga agtaagagga gatcccgctc ctcagggagc
       61 cctttgaaca gagcattgag aggaattccc tgttcgggct gcgtgcgcgg cgcttccgcg
      121 cctcgtttcg gctgatccag aagcgccgga tagcgttcaa tcagccgagc ctcgctgatc
      181 cccagccgtt ccgccacgat tttgatgcgc tcctgccgca cgatcggatg ctcgctcttc
      241 tgcagaattc gcagcacctc atcgacgctt cggatgcggc tttcaagcga gccgtcgacg
      301 gcctgcttga ctgtatgatt cagcgcatag tccaagagac tcggagcagc cgcttccagc
      361 tgggcaaatg ccgcggcacc ttccttccgc acaaacgtat cagggtcctc accaaccgga
      421 agggttacga ctttgacgcc caatccgctg ttgacgaaaa gatccaatcc tctcaacgcc
      481 gcgcgcacac cggctgcatc agggtcaaag agcaacacga cctggtcggc aaaccgccgc
      541 agggcttgaa tatgttcagc cgtcaaggcc gtccccagcg tggcgacggt atgggtaagt
      601 cctgcttggt ggagcgcgat cgcgtcgaaa tagccttcga cgacgatcac tgtcttggtc
      661 cgcacgatcg cctcgcgcgc ctgatcgaag gcgaaaagag tttggccctt cttaaacaac
      721 aacgtatcgg gcgaattgag atacttgggc atgcggtcgt ccaacacacg tccgccgaat
      781 cccaccacgc gcttgcgcag atcggcgatc gtgaacatga gtcgtccatg gaacttgtcg
      841 tagcacccat taccgttttg tctcgggctt ccgagcccag ctgccatgat ctcgctacga
      901 gagaatccat ttcgagaaag gaatttacac aacccatccc actccgggga cgacactcca
      961 atttggaatg catccactgt cgattgttga atgcctcgtt tgttcagata ctcacgagct
     1021 gtggcaccga tttgcagatc acgcagattg gcttgaaacc acgtcatcga ggctcgattg
     1081 atttcttcaa tgcgactggt ctgcccgcgt tgcactcgtt ccgccggcga ttcttccacc
     1141 gcaatgccca ccgtgtctcc aagctcccgc agcacttccg gaaaagttgc tccagtaatt
     1201 ttgctcagaa aagcgaagac atctccccca gtcttacagc cgaagcagta aaacatttgt
     1261 ttggaaggac tgaccgaaaa agaagggctt ttttcctgat gaaacgggca caaccccaca
     1321 aggttctgcc cagccctcct cagcgaaata tggcgcccga cgacatccgc gatatctact
     1381 cggtctctta tgcgattctt gatatcgtcg gaaatcaagc ttcggcccac cagagtcgca
     1441 gctcctactt tcccgtcgca cacgaccgga cgagtcggtt ggtaagtaat cggaataatt
     1501 gaccgttcag attaacagac gggtggaaaa cctgtcaatt ctgagaattc cgtcaccctg
     1561 gaggccaggc catatgccgc cctccgagca catgaaaatg aagatgaaag acggtctgcc
     1621 cggcatcccg accggtattc gcaaccaacc gatagccgga ttcccgtact cccttcaggg
     1681 ctgcgacctt cgtacagctg atcagcaatc gtgcaagtaa cgagtcatcg cccatcccta
     1741 aatcctgcac ggccgaaata tgccgcttcg gtatcaccag agtatggacc ggcgcttgag
     1801 gattgatgtc gtcaaaagcc agtgtgtgct catcctcttg aactagcttc gccggaatct
     1861 tcttctctac gatcttacaa aataggcact cgctcatctc aacctctaca ataattcaac
     1921 gtcatcctcg gatccgtctg ataataccct atgactattc ggctatttgc ctgaccttag
     1981 acccgatttt ccaaatcggc ttcccaattc ttcataaatg tcctgcaaag acaagtcgtg
     2041 gtagcccagc accatgaggg tatggaagaa cagatcggcg acctcgtaca cgatttcctc
     2101 tttcttgcca cctttggacg ccaacaggac ttctccggcc tcctccgcca cctttttcag
     2161 aatcttgtca tgccccccct cgaacagctt tgaggcatag gaaccagtct gggggctgga
     2221 gcggcgctcc cgaatcgtct gtagaatacg ttcaagaatt ccaccctgcg cgtcttgcgc
     2281 cttccgatga acgacgcggc cttgttcatc tattctagaa aagaaacagg cccgttcgcc
     2341 cgtat
//
LOCUS       DASTWE010000025         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1196359, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000025 DASTWE010000000
VERSION     DASTWE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196359"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VFT30_00280"
     CDS             <1..1008
                     /locus_tag="VFT30_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5091086.1"
                     /translation="IAAVVSRWTGIPVSRLLEGETDKLLKLEELLHQRVVGQDEGVRA
                     VADAVLRARSGIKDPNRPIGSFLFLGPTGVGKTELARALAAILFDDEGNLIRIDMSEY
                     MEKHTVARLIGAPPGYIGYEEGGQLTEAVRRHPFSVILFDEIEKAHHDVFNILLQVLD
                     DGRLTDSQGRTVDFKNTVLIMTSNIGSPHILEAQQRGASHEQVSTVVMGELRQHFRPE
                     FLNRVDELVVFHPLGTEHLIKIVEIQLDRLRNRLAERRIDLALSPSALRHLGERGYDP
                     VYGARPLKRLIQQELETPIARLLVKGELRDGDTVSIDLKNGSLILVPTTTEQRTSSPS
                     R"
     gene            complement(998..1423)
                     /locus_tag="VFT30_00285"
     CDS             complement(998..1423)
                     /locus_tag="VFT30_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091087.1"
                     /translation="MDIEIGSNLYRNSNGTIEVDGVPQIQVARHPSTGALLVNFALFD
                     SNGRMLAKVVDSTMMYNERRAYELMKTNKSVAMKEAESGKILLHFEMKAADVVSLSRG
                     EFHTMKGRLLHVSPTEWKIEKQQQSGLTQDAKGDAVSIG"
BASE COUNT          362 a          443 c          411 g          351 t
ORIGIN      
        1 attgcggccg tggtcagccg atggaccggt attcccgtct ctcgtttact tgaaggggaa
       61 accgacaaac tgttgaaact cgaggaactg ctgcatcaac gtgtcgtggg acaagatgaa
      121 ggcgttcgag cggtcgcgga cgctgtgctt cgtgcgcgat cgggcatcaa agatcccaat
      181 cgcccgatcg gttcatttct gtttctcggc ccgaccggtg tcgggaagac cgaactcgcg
      241 cgtgcgctgg ccgcaattct gttcgacgac gaaggaaatc tgatcagaat cgacatgtcc
      301 gaatatatgg aaaagcacac ggtggcccgc ctcatcggcg cacctccggg ctacatcggc
      361 tacgaagaag gaggccaact caccgaagcc gttcgacggc acccattctc cgtaatcctg
      421 ttcgatgaaa tcgaaaaggc acaccacgat gtcttcaata ttttgctgca agtgctggac
      481 gatgggcgac tgactgattc acaaggccgc acggtcgatt tcaagaacac cgtgttaatc
      541 atgacctcca acatcggcag cccgcacatc ctcgaagcac aacagcgagg tgcgtcgcat
      601 gaacaagtca gcacggtcgt catgggggaa ctgcgccaac attttcgccc ggagttctta
      661 aaccgggtcg atgaactcgt ggtgttccat ccgctcggca ccgaacactt gatcaagatc
      721 gtggaaattc agctggaccg tctccgcaat cgattggccg agcggcggat tgacttggca
      781 ctcagccctt ccgcactcag gcatctcgga gaacggggtt acgacccggt ctacggggca
      841 cggcccctca agcgcctcat tcagcaggaa ttggaaacac cgatcgcgcg attgctggtc
      901 aaaggagaat tgcgggacgg tgataccgta tcgattgatc tgaagaacgg aagtcttatt
      961 ctggtgccga cgacaactga gcagcggaca tcttcgccta gccgatagac acggcatccc
     1021 ctttggcatc ttgggtaagc ccgctctgct gctgcttctc aatcttccac tccgtcggcg
     1081 acacgtgcag caggcggcct ttcatcgtgt gaaactctcc gcgcgacaaa gacaccacgt
     1141 cggcggcctt catttcgaaa tggagaagaa tttttccgga ctcggcttct ttcatcgcca
     1201 cgctcttgtt cgtcttcatc aattcatacg cccgccgttc gttgtacatc atggtgctgt
     1261 ccaccacttt cgccaacatt cggccattcg agtcaaacaa cgcaaagttc accaggagag
     1321 ccccggttga aggatgtcgt gcgacttgaa tttgcgggac accatcgact tcgatggtgc
     1381 cgttggaatt tcggtaaaga ttggagccga tttcgatatc catagtccct aagcctcagt
     1441 gtaatatctt tcaaccgtcc cttttatccc tgcggtgagc gagttcctcc acaaaggcct
     1501 ggtgtcaaaa tgtaggagaa gaatcagtcc aaacgtgacg agggctatag atatttgcac
     1561 atacact
//
LOCUS       DASTWE010000026         1460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_97623, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000026 DASTWE010000000
VERSION     DASTWE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97623"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="trpD"
                     /locus_tag="VFT30_00290"
     CDS             <1..1135
                     /gene="trpD"
                     /locus_tag="VFT30_00290"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5091088.1"
                     /translation="TAPLQWHPAMIRGPVAGRILPRPNAGYTSRNMPIQNLLAKIAKG
                     PKASKDLTWDECKQAMKALIEGEATPAQVGAFLIAMRFKMESVTELAALTAAARQYVP
                     PLTVSRDLALVDVPTYAGKQDTFHAIIASSIVAVAAGAAVLMHGYDGIPGRPGSAGVL
                     KALGIPVDADPKLVSEDVNKKGFAYLDIGLYHPPLYRFLEMRQELGVRNVFHPIARLL
                     NPARAAVQVVGLTHPPHFEKTAEVLRLLGCRRALVIRGVEGDPELSVAMATRVLELKD
                     ERITPLGVAAKDFGLAFASSREMAGFPPDQREREADLIRRILRNQVQGGPRDWVLMNA
                     AMLLYAVGKGVSLSACFPAVRAALDGGAAGRKLDELVRQPVAA"
     gene            1154..>1460
                     /locus_tag="VFT30_00295"
     CDS             1154..>1460
                     /locus_tag="VFT30_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEU5091089.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFDNLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPAMIEYRDRLAREWRLNLVVGQNKEALTAGMNHTMGRVV
                     "
BASE COUNT          312 a          372 c          447 g          329 t
ORIGIN      
        1 tactgcaccg ctccagtggc atccggcgat gatccgcggg ccggtcgctg gaagaatttt
       61 gccaagaccg aatgcgggct acacaagtcg taacatgccg attcaaaatc tcctcgccaa
      121 gattgccaag ggcccaaaag cttccaagga tctcacatgg gatgagtgca aacaggcgat
      181 gaaagctttg attgaaggtg aggccacgcc ggcacaagtg ggggcctttc tcattgccat
      241 gcgattcaag atggaatcgg ttaccgagtt ggcggcactg acggccgctg ctcgacagta
      301 tgtgccgccg ctcactgtct cacgggactt ggccttggtc gatgtcccaa cctatgccgg
      361 gaagcaggat acgtttcatg cgattattgc ctcgtccatt gttgcagttg cagccggcgc
      421 tgcagtcttg atgcacggct atgacggcat tcctggccga ccaggcagtg ccggagtact
      481 gaaagccttg ggcattccag ttgatgctga tcccaagctg gtttccgaag acgtgaacaa
      541 gaaaggcttt gcctacctgg atatcggact gtaccatcca cccctctatc gatttctgga
      601 gatgcgtcaa gagctcgggg tcaggaatgt gtttcatccg atcgccaggc ttctgaatcc
      661 ggctcgggcg gcagtgcaag tcgtggggtt gacgcatccg ccgcattttg aaaaaacagc
      721 cgaagtcttg cgactcctcg ggtgtcggcg agcattggtg atccgtggag ttgaaggtga
      781 tccggaattg tctgtcgcga tggcgacgag ggtcctggaa ttgaaagatg agcgcattac
      841 gccgctcggt gtggccgcga aagactttgg attggcattc gcctcatcgc gcgagatggc
      901 ggggtttcct cctgatcagc gggaaaggga ggccgatctg atccggcgaa ttcttcgaaa
      961 ccaggttcag ggtggacctc gagattgggt gctgatgaat gcggccatgt tgctctatgc
     1021 ggtgggcaaa ggagtgtctc tgtcggcctg ctttccggcg gtacgcgcgg cgcttgatgg
     1081 aggagctgca gggcgtaagc tcgatgaatt ggtgcgacag ccggtcgctg cgtaagacct
     1141 aaggaacaag ccaatgaaac atcttcggca acttgaagat caaagcgtct acatccttcg
     1201 tgaagcctat aaacacttcg acaatctcgc catgctctgg tccatgggca aggattcaac
     1261 ggtattgctg tggctagcgc gcaaggcctt cttcgggcat gtgccgtttc cgcttttgca
     1321 cgtcgatacc agctacaaaa ttccggcgat gatcgagtac cgagaccgtc ttgcgcggga
     1381 atggcggttg aatctggtgg tgggtcaaaa caaagaagcc ttgacggcag ggatgaatca
     1441 cacgatgggc cgggtcgtct
//
LOCUS       DASTWE010000027         1119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_259204, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000027 DASTWE010000000
VERSION     DASTWE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259204"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFT30_00300"
     CDS             <1..651
                     /locus_tag="VFT30_00300"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU5091090.1"
                     /translation="KKLIAKGRKDDVLVLHYSGHGSNVPDDNGDEADHRDEILCPTDL
                     DWKDPFRDDWLRKTLGKLRSGVNLTVIMDCCHSGTITRVMNPFDAPRRQRYLPCPLDL
                     MATESGRKLRGTLRGQLGKAPRGRKRKKDIVHADIQELLITGCRDTQTSADAYIGGSY
                     NGALTYYLVESIKEAEGKLTYRELHQRTAAKLKQEDFDQIPQLEGRRTSFDRLFLS"
     gene            744..>1119
                     /locus_tag="VFT30_00305"
     CDS             744..>1119
                     /locus_tag="VFT30_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091091.1"
                     /translation="MSKKPKATMRGDSDRRIPAMDWEEFEAQLSRSASRGTSRTVTDQ
                     ALRDYFGPEKLERLQQLAQRVRSARQKREPLRGNIIFIPGIMGSELTVTDKGDDDVVW
                     ISFWRLIKGGIKKLQLAADGVQE"
BASE COUNT          268 a          284 c          329 g          238 t
ORIGIN      
        1 aaaaaactaa ttgcgaaagg gaggaaggac gatgtgctgg tcctccatta ttccgggcat
       61 ggatcaaacg tgccggatga caatggggac gaagcagatc atcgggatga aatcctgtgt
      121 ccgaccgatc tggattggaa agatcccttc cgcgatgatt ggctacggaa gaccttggga
      181 aaacttcggt cgggggtaaa cctcactgtc atcatggatt gctgtcattc cggcaccatc
      241 acgcgcgtga tgaatccgtt cgatgctccc agaaggcaac ggtacctccc ttgtccgttg
      301 gatctcatgg cgaccgagtc tggcaggaag ctgcgtggga cacttcgcgg tcaattgggc
      361 aaggctccgc gtggacgtaa gcgaaagaag gacatcgttc atgcggatat tcaagagttg
      421 ctcatcaccg gctgtcggga tacgcaaacc tcggccgacg cgtatatcgg tggttcctat
      481 aatggtgccc tcacctatta cctggttgag tcgatcaaag aggccgaggg caagctgacg
      541 tatcgagagc tgcatcagcg gactgccgca aaactgaagc aggaggattt cgaccaaatt
      601 ccgcagttgg aaggacggcg tacgtcgttc gatcgtctct ttctgagcta gaaaagcacg
      661 gccacggccg ccatcaagcg ccgccgtggc catagagggt ggtatacgct gcttgactcc
      721 ttctccattg gaggaattcc ctcatgagta aaaagccgaa agcgaccatg cgtggcgatt
      781 ccgaccgtcg gatccctgcc atggattggg aggagtttga ggctcagctc tctcgttctg
      841 ccagcagagg aacttcacga accgtaaccg atcaggcctt acgggactac tttgggcccg
      901 aaaagctcga gcggctccaa cagctggcgc aacgggttcg ctcagcccgt caaaaacggg
      961 agccgcttcg gggcaatatt atctttatcc ccggcatcat gggctccgaa ttgaccgtga
     1021 ctgacaaggg tgatgatgac gtcgtgtgga tcagtttctg gaggcttatc aaaggtggga
     1081 ttaagaaact acaacttgcc gcagatggag tgcaggaag
//
LOCUS       DASTWE010000028         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1293317, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000028 DASTWE010000000
VERSION     DASTWE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293317"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFT30_00310"
     CDS             complement(<1..478)
                     /locus_tag="VFT30_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5091092.1"
                     /translation="MTDRRPDTTDSQAISIDSDCWAFAFEGSGIGLWDWNITTNKVLF
                     SSGWKDLFGYAQDEVGDNFNEWSSLIHPEDLSTAMANIQRYVQGESSVFTSEHRIRCK
                     DGSYKWVLERGKIVSQTKNGKSLRVLGIHTDITERKHLERRLTIQHEVAKVLARASG"
     gene            complement(834..>1678)
                     /locus_tag="VFT30_00315"
     CDS             complement(834..>1678)
                     /locus_tag="VFT30_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5091093.1"
                     /translation="IRMSPKARQSKGKARLNRYEELVTQKQDQVADDLEIYIPPGPRL
                     GDVVVEANGISKAFGDNVLYEKVNFNLPKGGIVGVIGPNGAGKTTMFKMIIGKEKPDA
                     GSIKVGETVKLGYVDQDRSLDGNKSVYEVISDGQDTIKLGKAEVNARGYCARFNFAGT
                     DQQKKVKDLSGGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEGLENFAGCA
                     VISSHDRWFLDRIATHILAFEGDSKVVWFEGNYSEYEADRKKRLGKEADQPHRIRYRK
                     LTRN"
BASE COUNT          338 a          472 c          446 g          422 t
ORIGIN      
        1 atccggatgc cctggccaac accttggcta cctcatgctg gatggtcaat cgtcgttcca
       61 aatgcttacg ttcggtaata tccgtatgaa taccaaggac gcggagagac ttgccgttct
      121 tcgtctgcga aacgatcttc cctcgttcga gcacccattt atatgaccca tccttgcatc
      181 gtatccggtg ttcactcgta aaaacggaac tctcgccctg aacgtatcgc tgaatattgg
      241 ccatggccgt tgaaagatcc tccggatgga tgagagagga ccattcgttg aaattgtcgc
      301 cgacttcgtc ctgtgcatag ccgaataggt ctttccaccc gcttgagaat aagactttat
      361 tggtggtgat attccaatcc cacagaccga tgccgctgcc ttcgaaggcg aacgcccagc
      421 agtcgctgtc gattgatatc gcctgtgagt cagttgtgtc agggcgccga tcggtcatga
      481 gacaccgttc ccgttaggat gggaacccat gtagcgtagg ctcaaagatt gattgatagc
      541 gtcatgaagg gctcgacgat gcccttactg tcaattagta tcggtttgtg aatcagggac
      601 cttgagaaga attgagcgga tggaccccct ctattgccat ggcattcggc cctagcaggc
      661 ctttgggcct actgggaggg agtaggattt cagacgtcgt aatgagggct tacaacggtg
      721 gcaacaagag ctgagggcag gatgacggca tctcgcggca tgggaatacc accggctctg
      781 cggttcggac agcaatgagg ccgtgcaaga gaggctatgg ccaagaataa ttttcagttc
      841 cgcgtcagct tccgatacct gattcggtgt ggctgatccg cttctttgcc caagcgtttc
      901 tttcgatccg cttcgtactc actgtagttt ccttcgaacc agacgacctt gctgtcgccc
      961 tcgaaggcga gaatgtgcgt agcaatgcgg tcgaggaacc agcggtcgtg gctgctgatt
     1021 acggcacagc cggcaaaatt ttccagccct tcttcaagag cgcgcaaggt attcacgtcc
     1081 agatcgttgg tcggctcgtc aaggatgatc aggttcgctc cctctttcaa catgcgggcg
     1141 agatgcactc gattgcgttc gccgcctgag agatccttca ccttcttctg ttgatccgtc
     1201 ccggcaaagt tgaagcgggc gcagtagccg cgcgcattca cttcggcctt gccgagcttg
     1261 atggtgtcct gaccatcgga gatgacctcg tacacgctct tgttgccgtc gaggctccga
     1321 tcctgatcca cgtagccgag cttgacggtc tctccgacct tgatcgaccc ggcatccggt
     1381 ttctctttgc cgatgatcat cttgaacatc gttgtcttac cggcgccgtt gggaccgatc
     1441 acccccacaa ttccaccttt cggtaagttg aaattgactt tctcgtagag cacgttgtcg
     1501 ccgaaggcct tgctgatgcc gttcgcttcg accacgacat cgcccaaccg cggccccggt
     1561 ggaatgtaga tttccagatc atccgccacc tggtcctgtt tttgggtgac cagttcttca
     1621 taacggttca agcgcgcctt gcccttggac tgccgcgcct tcggggacat ccgaatcc
//
LOCUS       DASTWE010000029         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1261077, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000029 DASTWE010000000
VERSION     DASTWE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261077"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFT30_00320"
     CDS             <1..257
                     /locus_tag="VFT30_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEU5091094.1"
                     /translation="LCELIGMKRYGECQVVDFGEGRVAGYSMVQLISTSLISGHFANE
                     TNHAYLDIFSCKGYDPTVVESFSKEFFGARRSVATATLRY"
     gene            352..690
                     /locus_tag="VFT30_00325"
     CDS             352..690
                     /locus_tag="VFT30_00325"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEU5091095.1"
                     /translation="MKVTTVKEVLDSLPSKLDKDAAEDLDAIYQFDLRGNQGGQYHLL
                     VQNGTCVVKNGAHADPHVTLSMTGEDCIGILNGQVSGMTMAMSGRLQVTGDIGLAMQL
                     KSLFPNVVEG"
     gene            complement(687..>1875)
                     /locus_tag="VFT30_00330"
     CDS             complement(687..>1875)
                     /locus_tag="VFT30_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5091096.1"
                     /translation="GLIKRVTSFGSQKAVDVEIYGYDFEKARDVIARVKEIMEQIPGL
                     ADIEPSREENYPEVNVTVDREKAALLGISEADVANAVLFSLNGNGQTDPIIYTDPQSG
                     NEYFISAWLAEEHRKSLTDLENILLTTKAGEPVLLKNVASLKLNAGPVKVDRKYFQRV
                     VHITANPTTRPLGDIAEDLESALATLQLPAGFSIKLAGQIQQQRETFQGLQFAIILAL
                     LLVYMVMAAQFKSLIDPFIIMFSVPMGFPGVILILFLTNTTLSTTSMMGIIMMFGIVV
                     SNGVLLVDYTNVLRRKGEPLHQAVVTAARTRLRPILMTSLATVFGLLPMAIGLGTGGE
                     TNAPLARAVVGGLSVSTLLTLFLVPTMYAILEERFPRRLDWEMRNHQQAQAAELLAGS
                     SIH"
BASE COUNT          419 a          492 c          529 g          435 t
ORIGIN      
        1 agctctgtga gctcatcggc atgaagcgct acggtgagtg ccaggtcgtc gattttggcg
       61 aaggccgagt cgccggatac tccatggtac agctgatctc cacctcattg atcagcggcc
      121 actttgctaa tgaaaccaat cacgcctacc tcgacatttt cagctgcaag gggtatgatc
      181 ccacggtcgt cgagtcgttt tcgaaggagt ttttcggcgc ccgtcgcagt gttgcgactg
      241 ccacactccg gtactaaact ttttttcaca gtgaccgtgc ttcgggggcg ttaggttagc
      301 gtaacgcccc cgaatgcttt aaccatccaa tatcaggggt atcgtccaga catgaaagtc
      361 acgactgtca aagaggtgct cgactcactt ccatcaaaac tcgataaaga tgcagccgaa
      421 gatcttgatg ccatctacca gttcgactta cgagggaacc aaggaggcca gtaccatctg
      481 ctggtgcaaa atgggacctg cgtcgtgaag aatggagccc atgccgatcc gcatgtgact
      541 ctatcgatga cgggagaaga ctgcatcggg attctaaatg gtcaagtcag tgggatgacg
      601 atggcgatgt cggggcgtct gcaagttacc ggggatatcg ggctggctat gcagctgaaa
      661 tccttattcc cgaacgttgt tgaaggctaa tgaatgctac tcccggccag gagctccgcg
      721 gcttgagctt gctggtgatt ccgcatttcc caatcaagtc ttctcgggaa ccgttcttct
      781 aaaatcgcgt acatcgttgg gacgagaaac aacgtgagga gcgtagagac gctcagccct
      841 ccgacgaccg cccgtgccag gggcgcgttg gtttcgccgc cggttccaag gccgatcgcc
      901 ataggaagca gaccgaagac cgtggccagt gaggtcatga gaatcggacg cagtctagtt
      961 cgcgcggcgg tcacgacggc ctggtgaagc ggttctccct tgcgacgcag cacgttggtg
     1021 taatcgacca gcaggacacc gtttgaaacg acaatcccaa acatcatgat gatgcccatc
     1081 atcgatgtgg tggacagcgt cgtattcgtc aggaacagta tcaaaatgac acccggaaaa
     1141 cccatcggca ctgaaaacat gataatgaat gggtcgatga gcgacttaaa ttgtgcggcc
     1201 atgaccatgt agaccaacaa gagcgctaag atgatcgcaa actgcagtcc ctggaaggtt
     1261 tcgcgttgct gctgaatctg ccctgccagc ttgatgctaa agccggctgg cagctggagt
     1321 gtggctaatg cggactcgag gtcttcggcg atatcaccga gaggccgggt ggttggattg
     1381 gccgttatgt ggaccacgcg ctggaaatac ttccgatcga ctttcactgg tcccgcgttc
     1441 aatttgagag acgccacatt cttcagtaag accggctcgc cggctttggt agtgaggaga
     1501 atgttctcca gatcggtgag actttttcgg tgttcctcag cgagccacgc gctgatgaag
     1561 tattcgttgc cgctttgcgg atcagtataa atgatggggt cggtttgacc gtttccgttc
     1621 agggaaaaca ggaccgcatt ggctacgtcg gcctcgctga tgccgagcag agcggccttc
     1681 tctcgatcga ctgtgacgtt gacctcggga tagttttctt cccgactcgg ttcgatatcc
     1741 gccaggcccg gaatctgctc catgatctcc ttgacgcggg cgatgacgtc tctcgctttt
     1801 tcgaagtcat agccatagat ttccacatcc acggcctttt gggagccgaa gctagtgacg
     1861 cgcttgatga gacca
//
LOCUS       DASTWE010000030         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1584287, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000030 DASTWE010000000
VERSION     DASTWE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584287"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..962
                     /locus_tag="VFT30_00335"
     CDS             282..962
                     /locus_tag="VFT30_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5091097.1"
                     /translation="MTTNVITVYPDTSIQYIAKRLIENRISAVPVLEMDGRLVGIVSE
                     GDLMRRSESGTERHSSWWLFLLESPEKTARDYVKTHGSRAADVMTRQVITVDENMSLP
                     EVAETLEKNLIKRVPVMSGDKLVGIISRANVIRGLAAQRVATESVTDDRTIKASVEKA
                     LSAAGVRRAFLDVTVQGGVVTLWGMVESPAERQAARVAAESAPGATKVQDNLSIMPPI
                     VRPIMWAE"
BASE COUNT          262 a          269 c          291 g          210 t
ORIGIN      
        1 gaccccccgg ctttgtccac tcgaacggcg tcgagtgctc aagggtggtc tctgcgccac
       61 gattccacgg cccgatatct tttgaactga ttttgcgaca gggtttgaaa gaaatccctc
      121 cgtaggacca ttagagcaca ccgttgatta ggatcaacgc tgccgttgtt gcagcaacct
      181 atgaaaccat agggatatga gttgaggctg gccggccgct tgacgaaacg gcaccctaac
      241 ggatgtcgtt aagggaggat caaatgcaag cgaaagatat catgactacg aacgtcatca
      301 cagtctaccc ggacacgagc atccaatata tcgccaaacg cttgatagaa aataggatca
      361 gcgctgtccc tgtcctggag atggacggcc ggcttgtcgg catcgtgagc gaaggggatc
      421 tgatgcgccg atccgaatcg ggcaccgaac gccattcctc atggtggctt ttcctgttgg
      481 aaagcccaga aaaaacagca cgggactacg tcaagactca cggcagccgc gcggcagacg
      541 tgatgacccg tcaggtgatc acagtcgatg aaaacatgtc acttccggaa gttgccgaga
      601 cgcttgaaaa gaacctcata aaaagggttc ctgtcatgag cggcgacaag ttggtcggaa
      661 taattagccg cgccaacgtc atccgcggtc ttgctgctca gcgagttgca acagaatccg
      721 tcaccgatga cagaacaatt aaggcgtctg ttgaaaaagc tttgtcagca gccggtgtgc
      781 gtcgggcatt tctggacgta actgtccagg gcggcgtggt gacactttgg gggatggtcg
      841 agtctcccgc ggaaagacag gcagctcgcg ttgcggcaga atcggcaccc ggcgccacaa
      901 aggtacaaga taatcttagt attatgcctc caattgttcg cccgattatg tgggcggaat
      961 gacagacttg gaaataggaa tcgcgcatac ccgccgttcg caagcgagca gcagtaaaac
     1021 gccgatgtcc cg
//
LOCUS       DASTWE010000031         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_841017, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000031 DASTWE010000000
VERSION     DASTWE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841017"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFT30_00340"
     CDS             <1..258
                     /locus_tag="VFT30_00340"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEU5091098.1"
                     /translation="IVWFGESLFAEDLDCCSRVLKHCDVLLVIGTSGVVYPAAGFASV
                     AKEAGAFVAEINPDPTPQSALVDISLHGRAKDLVPLLFKPI"
     gene            complement(238..963)
                     /locus_tag="VFT30_00345"
     CDS             complement(238..963)
                     /locus_tag="VFT30_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5091099.1"
                     /translation="MSSSIRVVFFDAADTLFHVHGSVAEIYLRHAVEFGFPQKPDSLL
                     AIKQAFSRAFHEAPPPVFSAVEPARIKQSERLWWFDIVHHVFYRVGMFERFDAFFDHV
                     FRVFEDHRSWCLFPETASTLSQLKARDLELGIISNFDSRLFGVMRGLGIADAFDTVTI
                     SSLAQAAKPAPQIFHIALEKHAVDPEEAFHIGDSLRDDVEGAKKAGLRAILLDRDGIQ
                     DGAGVQTIKSLEELLPLLDRFEE"
     gene            1105..>2159
                     /locus_tag="VFT30_00350"
     CDS             1105..>2159
                     /locus_tag="VFT30_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091100.1"
                     /translation="MASPIFVVDSSPAVRRLVEQISIPEGFEVVGFQDGPAALEAARR
                     SSPSLIIADYHLDNMTFSGFCKEINKLDNLTETYLISLVNPADRPDENHLRTLGVKAF
                     LKKPFQSDDLLGVLKSLQQNQAGASNGKILKRRVWPPTSTLTDSDDEAIDYPTSGAGQ
                     EEVMDQLGSSSSASPAPPITPAAAPEEAMRGLFDQLLQSMTIRSQQSLAELLPRVIDE
                     KLATHVRPTVQKELQAQLGSILSEEYLTAIIQPLVSQALPALIRKELETSEPMIRQTV
                     SDLAKAFTGESVDRLVKDQVESGIHQYLPAVVREEIGSVDQLVKDELQREVLRQAPLL
                     ADDLVRTTVGQTVEQAV"
BASE COUNT          528 a          614 c          558 g          459 t
ORIGIN      
        1 attgtctggt tcggagaatc cttatttgcc gaggatctcg attgctgctc cagggtgctc
       61 aaacactgcg acgtcttgct cgtcatcggc acatccggcg tcgtctatcc ggctgcgggg
      121 ttcgcatccg ttgccaaaga agctggtgca ttcgtcgccg aaatcaaccc cgatcctaca
      181 ccgcagtcgg cactggtcga catctcacta cacggccgtg caaaagatct tgtgccatta
      241 ctcttcaaac cgatctaaga gtggcaacaa ttcctccagg ctcttaatgg tctgaacacc
      301 tgctccatcc tgtattccgt cacgatcgag taggatagcg cgcagtccgg ctttcttcgc
      361 accctccacg tcgtctcgca gactgtctcc gatatggaac gcctcctccg gatcgacggc
      421 atgcttctcc aacgcgatgt gaaaaatctg gggagcaggt ttcgcggctt gggccaaact
      481 tgagatggtc accgtgtcga atgcatcggc gatcccgagt ccacgcatca cgccaaatag
      541 gcgggaatcg aaattcgaga tgatcccgag ctccaaatcc cgtgctttga gctgagacaa
      601 agtggaggca gtttccggaa acaagcacca tgatcgatga tcctcaaaca cacgaaacac
      661 gtgatcgaag aacgcgtcga atcgctcgaa catgcccaca cggtagaaga cgtgatgaac
      721 gatatcgaac caccacagcc gttcactctg cttgattcgt gccggttcaa ccgcagaaaa
      781 gaccggcggc ggggcttcgt ggaacgctcg gctgaacgct tgtttgattg ctagcaacga
      841 gtctggtttc tgcggaaaac cgaattcaac cgcatgtcga agataaatct cagcgaccga
      901 cccgtgcacg tgaaagaggg tatcggctgc atcgaaaaac acaactcgga tcgatgaact
      961 cataatgggt ccgccctgat ctcagttcgg cacccggttc cgtgccgaaa tacatcgtga
     1021 atccaatcac ctatcgatgg ttttcttgac aaagaatacc ccccttgtta gcttcgaaga
     1081 aactaaaaag ttgggagagt tttcatggct tcgccgatct ttgtggtaga cagcagcccc
     1141 gcggtgagac ggctggtgga acagatctca atcccggaag gctttgaggt cgttgggttt
     1201 caagacggac cagccgccct tgaggccgct cgacggagtt cgccatcgct gatcatcgcg
     1261 gactatcatc tcgacaacat gactttttcg gggttttgca aggagatcaa caaactggac
     1321 aatctgacgg aaacgtatct tatttcgttg gtgaatccgg ctgatcgccc ggacgaaaat
     1381 cacctccgca cattaggtgt caaagccttc ctgaagaaac cgtttcaatc tgacgatttg
     1441 ctcggcgtgc ttaaatctct tcaacagaac caggctggtg cctcgaacgg caagattctg
     1501 aaacggcgcg tctggccacc gacttcgact ttaaccgact ccgatgacga ggcgatcgac
     1561 tatccgacaa gtggcgccgg ccaggaggaa gttatggacc agcttggcag ctcatccagt
     1621 gcatcaccgg cgccaccaat cacccccgcc gcagccccgg aagaggccat gcggggcctc
     1681 ttcgaccaac tcttacaatc gatgaccatc cgaagccagc agagtcttgc cgagctgctc
     1741 cctcgagtga tcgacgagaa actggcgacc cacgtccgac caaccgttca gaaagagttg
     1801 caagcacagc tcgggagcat cctctctgag gagtatctca cagcgatcat tcagcccctg
     1861 gtctctcagg cattgcccgc tctcatcagg aaagaactag aaaccagcga gcccatgatc
     1921 cggcaaaccg tatccgattt ggccaaagct ttcaccgggg agagcgttga tcgactggtc
     1981 aaagatcagg tcgagtcagg gatccatcaa tatttacccg cagtcgtgcg ggaagaaata
     2041 ggttcggtcg atcaattggt caaagatgag cttcaacgtg aggtgctgag gcaggccccg
     2101 ctactggcgg acgacttagt gagaaccaca gttggacaaa ccgtcgaaca ggcggttca
//
LOCUS       DASTWE010000032         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1487304, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000032 DASTWE010000000
VERSION     DASTWE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487304"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFT30_00355"
     CDS             complement(<1..346)
                     /locus_tag="VFT30_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091101.1"
                     /translation="MQTFWSQRVVPAVLMIASLSVSLPASANPPTKERLPLMLSGSGC
                     GSKEAEMNNVLQAIPGVTGVYFNRVPDHVLVDITPSIVKPADVVNRVNNAATSWQCKV
                     ELMQSCITADMPS"
     gene            complement(421..873)
                     /locus_tag="VFT30_00360"
     CDS             complement(421..873)
                     /locus_tag="VFT30_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU5091102.1"
                     /translation="MSPRFLLALLMVSSFGLAYFPLHSVAEEGVIVDGAGYTLHDTES
                     IKGHDEQTIAKDPVCDSSKKPKIFHVEPDEAKPGHTITIKGENFGTRDCFRGVAFSAA
                     GSTKIDYKFVNDTTIEVIVPDVQPGMSFIDVVASGGNARSKAFLVQAK"
     gene            complement(896..1717)
                     /locus_tag="VFT30_00365"
     CDS             complement(896..1717)
                     /locus_tag="VFT30_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5091103.1"
                     /translation="MTVEQFVTRLLVGLALCVSHAVTYAQTAGNLIIAGNGPEQTTIE
                     ALARAFEKANPRAYVDILWDENSKPLQLVKTGQAQIAVTGFEDPALFSTQIGWDGIGI
                     LVHLSNFTKEVTRQQVADIFSGKIKEWSELGGPETKILLIDRPRDQNVREAFETQLGI
                     VGKIPDSAKVIGEDEAVVKTVVGTLPPLSAASYVSLSTSLSAVSGGVAVRLLPVDKVE
                     PEVPTVKDGRYSLRRPLLLLSKKEPNTLVEAFAKFALSPPGQTIIGEIYVPMPSK"
     gene            complement(1773..2087)
                     /locus_tag="VFT30_00370"
     CDS             complement(1773..2087)
                     /locus_tag="VFT30_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5091104.1"
                     /translation="MPLYEYRCGQCEKQFEASQSVYARVEDTECPFCQARQATRLMSS
                     FSSNVVGDRKPGFTELKAKAMNEERMQRFAKLPPLNIRPNMPPPDLTSESEPMPTEGS
                     TS"
BASE COUNT          480 a          568 c          609 g          531 t
ORIGIN      
        1 ccgatggcat atctgcagtg atgcaggact gcatgagctc cactttgcat tgccacgatg
       61 tggccgcatt gttgacacgg ttgaccacat ccgccggctt cacgatgctg ggggtaatgt
      121 ccacgagcac atggtcaggg acgcggttga agtagacgcc ggtcacgcct ggaatggctt
      181 gcaagacgtt gttcatctcg gcttccttgc tgccgcagcc ggatccgctg agcatcaggg
      241 gaagtcgttc tttggtgggt gggtttgccg acgcaggcag ggaaacggaa agacttgcaa
      301 tcatgagtac ggctgggaca actcgttggc tccagaatgt ttgcatggaa tgaactccca
      361 gacaagaaca gtgtatctgc gttcataaaa tacacgacgc ggaataatat tgtcgagtta
      421 ctacttggcc tgcaccaaaa aggcttttga gcgggcattg cctccgctcg cgacgacatc
      481 aatgaatgac atgccaggct ggacatcagg aactatcact tcgatggtcg tatcgttcac
      541 gaacttgtag tcgatcttgg ttgacccggc tgcgctgaat gccacgccac ggaaacaatc
      601 tctcgttccg aaattttcgc ctttgatggt gatggtgtgc cccggcttgg cctcgtccgg
      661 ctccacgtgg aagatctttg gttttttgct gctgtcgcag acggggtctt tcgcgattgt
      721 ctgttcgtcg tgacccttga tcgattctgt gtcgtgcagt gtgtagcccg cgccgtcgac
      781 gatcactccc tcttcggcaa ccgagtgtaa gggaaagtaa gcgagtccaa acgatgaaac
      841 cattaggagg gctaaaagga atcggggaga catcgtgacc tccttcgatg gggaattact
      901 tgctcggcat cggaacatag atttctccga taatcgtttg cccgggaggg gagagcgcaa
      961 attttgcaaa ggcctcaacc agggtgttcg gttctttctt cgaaagcagc aacagcgggc
     1021 gacggaggct gtaacggcca tccttcacgg tcggcacttc cggctcaacc ttatccacag
     1081 gcagcaagcg gacggcgacg ccgcctgaga cggccgagag gctagtgctc aatgaaacat
     1141 aggaagctgc agagaggggc ggaagggtgc cgacgacggt cttcacaacg gcctcatcct
     1201 ccccgatcac tttggcggaa tcaggaattt tgcctactat cccgagttga gtttcaaagg
     1261 cttcccgaac gttctggtcc cgtggcctat cgatcaggag aatcttggtc tccggtccac
     1321 ccagttccga ccactccttt atctttcccg aaaaaatgtc ggcgacttgc tgcctggtca
     1381 cctctttggt gaagtttgat aaatggacca aaatgccgat tccatcccaa cctatctgag
     1441 tcgagaataa agccggatct tcgaaccctg tgacggcaat ctgggcctga cctgttttga
     1501 ccaactgtaa cggtttggaa ttttcatccc acaagatgtc gacataggca cggggattgg
     1561 ctttttcaaa ggctcgagcc aaggcctcga tggtcgtttg ctccggccca ttgcctgcga
     1621 tgatcaggtt tccagctgtt tgagcgtagg tgacggcatg ggacacacag agggccagcc
     1681 cgacaagcag ccgggttacg aactgttcca cggtcatcga atagcctgtt tgcacaggaa
     1741 gtcacgatcg agctggtact ggtattgggt gctcaagaag tcgatccttc tgttggcata
     1801 ggctctgatt ctgatgtgag atccggcggt ggcatgttgg gtctgatgtt caacggcggc
     1861 aacttggcga agcgctgcat ccgttcctcg ttcatggcct ttgctttcaa ctcagtaaag
     1921 cctggtttgc gatcaccgac gacattggac gaaaacgaag acatgagtcg ggttgcctgg
     1981 cgggcctgac aaaatggaca ttccgtgtct tcgaccctgg catacacgga ctgactagcc
     2041 tcgaattgtt tctcgcactg tccacaacgg tactcgtaga gtggcacgga attgaatcct
     2101 ttctgtatct ctattgtaaa gatatcatgc ggaactcggt tcgggacttg acaagacgac
     2161 ctttcccaag cgatcttgac cggctcac
//
LOCUS       DASTWE010000033         1460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_65491, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000033 DASTWE010000000
VERSION     DASTWE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65491"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..155)
                     /locus_tag="VFT30_00375"
     tRNA            complement(80..155)
                     /locus_tag="VFT30_00375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Thr,seq:cgt)
     gene            complement(253..>1460)
                     /locus_tag="VFT30_00380"
     CDS             complement(253..>1460)
                     /locus_tag="VFT30_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091105.1"
                     /translation="PDESLTIEIEVKNEGTGEAKDVTVTAEGKAELAAVFPSEVQIGS
                     LQPRESKRTSITQRVTASQENLHGELTLNLRTSSPVVSVPPPKVFSFGVKPRVIDPAL
                     IPDVDNLPNSLAAFTQPKAVIISIGVGTFRDEQVPAVKYARHDAEVMAEYLRIIGGVP
                     GERIRILLDRQALVRDVEDTFERWLRKKVDRETVVYVFFAGRALVEAGTGAISLVPYD
                     GTLAGAQQLYSLVRLQEVLYRLPIRRAILMFDVSTDPSPGAALADIPSPAWESPVSEA
                     RKDVEMWMVGNRSLQEAHAYDEGKHGLFTYHLLRGLQGVADVDRDGTVVAGELCTYAR
                     GQAARVAREQFSNKQDALCLPGTGRGGMVRIHPVARGNNSKPVPMQKQPNPPTDSSGL
                     GPNPMRIGP"
BASE COUNT          304 a          431 c          397 g          328 t
ORIGIN      
        1 gtggtagcag tggcgtcttc tcagcctaac cttgtggctg ctactaacgt acaagtccct
       61 gaaattccat taaaggttat ggagccggcg actggaatcg aaccagcgac ctgcggttta
      121 cgaaaccgct gctctaccaa ctgagctacg ccggcacctt ggtgagacag aaagaagatt
      181 ggatgaggtc ttgtccggca tgatagcggc caccggcgag cactgtcaat gaagcagatc
      241 ggctcaccga ccttatggcc cgatccgcat tggattgggg cccagccctg atgagtcagt
      301 gggaggattt ggttgtttct gcatcggaac cggctttgag ttattgcctc tcgctaccgg
      361 atgtattcgc accatgcctc cccgtcccgt tcctggcaaa cagagcgcat cttgcttgtt
      421 actgaattgt tcacgagcca cacgcgccgc ctgaccccgc gcataggtac agagttcgcc
      481 ggcgacgacg gtcccgtctc gatctacatc ggcaacacct tgcaaccctc tgagaagatg
      541 gtacgtgaac aatccgtgct tgccttcgtc atacgcatgg gcttcttgca ggcttcggtt
      601 gcccaccatc cacatctcca catctttcct ggcttcgctg acaggagatt cccaggccgg
      661 cgaggggatg tcggcaagag cggcacccgg cgacgggtcc gtcgagacgt caaacatcag
      721 aatagcccgc cggatcggaa gtcggtagag aacttcctgt agccggacga gggaatagag
      781 ctgctgtgcc ccggcgagtg tcccatcata cggcacgaga gagatcgcac cggtcccggc
      841 ctctaccagt gctcgtccgg caaaaaacac atagacaacg gtttcccggt ccaccttctt
      901 tcgaagccat cgctcaaacg tgtcttccac atcacgtacg agagcctgtc ggtcgagcag
      961 gatccgtatg cgctcgcctg gaacaccacc gatgatgcgc aagtattcgg ccatgacttc
     1021 cgcatcatgg cgagcatatt tgaccgccgg gacctgttcg tcgcgaaacg tcccgacacc
     1081 gatcgaaata atgacggctt tcggctgagt aaacgccgcc agagaattcg gcaggttgtc
     1141 gacatccgga atcagcgcag ggtcaatgac tctgggtttt acaccaaagc taaagacctt
     1201 gggcggggga acggacacca ccggactgct cgtccgcaag ttcaacgtca attctccatg
     1261 aaggttctct tgggaggccg tcacccgttg tgtgatcgac gttcgtttgc tctctcgcgg
     1321 ttgaaggctg ccgatttgga cttctgaggg aaatacggcg gccagctcgg cttttccttc
     1381 agccgtgact gtcacgtctt ttgcttcccc agttccctcg ttcttcactt caatttcgat
     1441 cgtgagggat tcgtcaggct
//
LOCUS       DASTWE010000034         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_744157, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000034 DASTWE010000000
VERSION     DASTWE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744157"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1283)
                     /locus_tag="VFT30_00385"
     CDS             complement(<1..>1283)
                     /locus_tag="VFT30_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5091106.1"
                     /translation="LADMVATSLALNYEAKQSLLLLLDARGRLQALNKILAQEVDVLE
                     LEDEIHSRVQSEVDKSQREYYLREQMRQIQNELGEGDIFTRDANELKKKLEAANLPDE
                     PKLVALKELDRLNQMPPMAPEVGIIRTYIDWIVELPWTSTTPDNLDVRNAAKILDRDH
                     YGLKKAKERILEYIAVRSLKPKRERQPILCFVGPPGVGKTSLGRSIAESLGRKFVRVS
                     LGGVRDEAEIRGHRRTYIGALPGRIIQTMKRAGTANPLFMLDEIDKLYSDFRGDPASA
                     MLEVLDPEQNNAFSDHYLELPFDLSKVMFVTTANTLSTIPPALLDRMEVIEFPGYIEE
                     EKLEIANRYLIPRQMFENGVDELNIAFEPNAVRRVIREYTYEAGVRNLEREIGRVCRK
                     IARMKAEGRKFPTMVTMESIEKFLGPQQVFQTEAER"
BASE COUNT          263 a          380 c          341 g          299 t
ORIGIN      
        1 cgttcggctt ccgtttgaaa gacctgctgg ggaccgagga atttttcgat cgattccatc
       61 gtcaccatgg tggggaactt ccttccctcg gctttcatcc gtgcgatctt gcggcagacg
      121 cgcccgatct ccctctccag attacggacg ccagcctcat acgtatattc acgaatgacc
      181 cggcggaccg cattcggctc gaaggcgatg ttgagctcat ccaccccatt ctcaaacatc
      241 tggcgcggga tcaggtaacg gttggcgatc tcgagtttct cctcttcgat gtagccggga
      301 aattcgatca cttccatgcg gtcgagcaat gccgggggga tcgtgctgag cgtgtttgct
      361 gtggtcacga acatgacctt cgaaagatcg aagggcagct ccagataatg atcgctgaaa
      421 gcattgttct gttcggggtc gagcacctcg agcattgccg aggcaggatc accgcggaag
      481 tcggaataga gcttgtcgat ctcatccaac atgaacagcg ggttggccgt tcccgcgcgc
      541 ttcatcgtct ggatgatacg cccgggcaag gcgccgatgt atgtacgacg gtgaccgcgg
      601 atctcggctt cgtcgcgcac gccgcccaag gagacgcgca caaacttacg ccccagcgac
      661 tcggcgatcg aacgacccaa cgatgtctta cccacacctg gcggtcccac aaagcacaga
      721 atgggctgcc gctctctctt cggcttcagg gaacgtaccg caatgtattc caggatacgt
      781 tccttggctt tcttcaaacc gtaatgatcg cgatccaaaa tcttcgccgc gttcctcaca
      841 tccagattat cgggggttgt gcttgtccaa gggagctcga caatccagtc aatgtacgtg
      901 cgaatgatgc caacctcagg tgccatcgga ggcatctgat tgagacgatc caattcctta
      961 agagcaacga gctttggttc atcgggaagg ttcgctgctt cgagtttttt cttcagctca
     1021 ttcgcgtcgc gagtgaagat gtcgccttcg cccagctcgt tctggatctg gcgcatctgc
     1081 tcacgcagat aatattcgcg ctggctctta tccacctcgc tctgtacgcg cgaatggatc
     1141 tcatcctcca gctcgagcac gtccacttcc tgggcaagga ttttgttcaa ggcctgcagc
     1201 cgtccacgcg cgtcgagcag caaaagcagg ctttgcttgg cctcataatt caaagccagc
     1261 gaggtggcca ccatatccgc cag
//
LOCUS       DASTWE010000035         4613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_905745, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000035 DASTWE010000000
VERSION     DASTWE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905745"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1220
                     /locus_tag="VFT30_00390"
     CDS             78..1220
                     /locus_tag="VFT30_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU5091107.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDPIFAEVREG
                     LKWLFQTRNDVLMLAASGTGGMEGAVSNFLSPGDKALYVNGGKFGERWGKLCKTFGVQ
                     ANEIKVEWGHAVNPQQVSDALKKDPSIKAVYVQASETSTGVSHDVKAIGEIIKGYDNT
                     ILVVDAITALGVFDIKTDTWGLDVVITGSQKALMLPPGMAFVSVSDKAWALADKAKNA
                     AFYFNFKKERENQVKNQTAFTPTVSLIIGLQEVFRMMKAEGLEKMFARQGRLALAMRE
                     GVKAAGMALFPKESPSDALTAVCAPDGIDGQAIYKNLRVQYGMTAAGGQDHLKGKIFR
                     LSHMGYADSFDVITALAATEMVLKGLGHSIQLGSGVGKAQEILLAK"
     gene            1238..2836
                     /gene="serA"
                     /locus_tag="VFT30_00395"
     CDS             1238..2836
                     /gene="serA"
                     /locus_tag="VFT30_00395"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEU5091108.1"
                     /translation="MAAAGMKILISDSLSKQGVEALEKAGFTVVVKSKMPKEELFKEI
                     KDADGLIVRSGTKVTAELIAAAEKLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNT
                     VTTAEHTMSMICAMSRRIPQATASVKAGKWEKDKFMGVELCNKVLGIIGAGQIGSHLT
                     KMAQGIGMSVVAFDPYLAPERAERMGVTMLDLEELFRRADVISVHTPLTPETRGIINA
                     QAIAKMKPGVLIANCARGGIINEQDLCEALKTKRVAAAAFDVFEEEPVKPDNPLLALD
                     NFICTPHIGAQTTEAQENVAIGIAEQIVDYFTKGVAKGAVNIPSVAPELLPRLQPFLT
                     LAEKLGTLQSQLVQGGIERVTVEYSGEVATLSIAPLTIAVLKGLLTPIMEHPVNYVNA
                     PIVAKERGIEVKEIKSTDAGDFTSLIRVRVEAGKVSHQVAGTLYHKKEARVTEIDQFK
                     VEVVPEEHMLLIHNVDRPGVIGMVGKVLGDQGINIVRMQCALEKRGGDALLIIGSDTE
                     FPTVVLDQIRSSSNILSVKVANLS"
     gene            2859..4256
                     /gene="hisZ"
                     /locus_tag="VFT30_00400"
     CDS             2859..4256
                     /gene="hisZ"
                     /locus_tag="VFT30_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU5091109.1"
                     /translation="MASAPPMRRTSSGQFPLVREHSLVPVGMATILPDAARHVRHLEA
                     ELLAHFNRFGYDEIILPTFEYLDVLMPGLEPSLLENSYKIVDRTTGRILLLRPDVTAQ
                     IARTVAMGMVGTHFPLRLSYRATVFRYEPEHAGRDREIFQVGAELIGADDPSADSEII
                     MLLIECLRQVGLRSFKISLGHVGFFKGLLIRAGLSSEGQKRAEQAAARKDVPRLGEIL
                     SQERVTKRSAHRILDALELCGQAEVLSRGRALAKGERPLVQALDRLAHVYRLLCETGF
                     QEAVLLDLGEFRGFDYYDGIVFDVFTDGIGVELGGGGRYDHLIGRFGRNLPSTGFALS
                     VDRLFRGLNHSDRTKSAGPEVLAVGPLGLSTRLVSVAQQLRQAGIRTVQRIVNPSGRD
                     LIGAAVKAGLEAEIDTIIVVGTDSPSPEQVVVLHQGNMSMAGRSHASPAVRKKTVSIT
                     DLIASLRVDLSKGAV"
     gene            4253..>4613
                     /locus_tag="VFT30_00405"
     CDS             4253..>4613
                     /locus_tag="VFT30_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5091110.1"
                     /translation="MKSIGMVDLSQPKLPPQNLEAEQSVLGAILLDNAAMPKAMELLV
                     EEDFYRTSHKKVYQAMLELSDTGEVIDQITLTERLKSRGELETIGGAAYLAELVQIVP
                     SSANIRYHCKIVRDKAVA"
BASE COUNT         1063 a         1162 c         1368 g         1020 t
ORIGIN      
        1 gtctcccagc gcaaatgata gcgtctgcct ctcctagtgg taccccacca gaatcgcatt
       61 gtaaggagga tcgatcaatg ttgaagcggt atctcttggc tcccggccca acgcccgtcc
      121 ctccagaggt gttgctggcg atggctcgtc cgatgatcca tcatcgcgca cccgagttcg
      181 atccgatatt cgcggaagtc cgcgaaggac tgaagtggct gtttcaaaca cggaatgatg
      241 tgttgatgtt ggcggcgtct ggaacaggtg gcatggaagg cgcagtctca aactttttgt
      301 ctcctggtga taaggccttg tatgtcaacg gaggcaagtt tggggagcgt tggggcaaac
      361 tctgtaaaac ttttggagtg caagcgaacg agatcaaggt cgaatgggga catgccgtta
      421 atccacagca agtctccgat gccctgaaga aagatccttc gatcaaagcg gtctatgttc
      481 aggccagcga gacgtcgacg ggggtttccc atgacgtcaa ggcgatcggc gagatcatca
      541 agggttatga caatacaatt ttggtagtcg atgccattac agcgcttggt gtatttgata
      601 tcaagaccga tacgtggggg ttggatgtcg tgattaccgg ctcccagaag gccttgatgc
      661 tacctccagg catggcgttt gtcagcgtca gcgacaaggc ttgggccttg gccgacaagg
      721 ctaaaaatgc ggccttttat ttcaacttta agaaggaacg tgaaaaccaa gtcaagaatc
      781 agaccgcatt cacccccacc gtttcgctga tcatcggact tcaagaggtt tttcgaatga
      841 tgaaggcgga aggactggaa aaaatgtttg cacggcaggg tcgtctggct ctggcgatgc
      901 gagaaggcgt caaggccgcc gggatggcct tgttcccaaa ggagtctccc agcgatgccc
      961 tgacggcagt atgtgctccc gatggcattg atggacaggc tatctataaa aatctccgtg
     1021 tgcaatacgg catgacggct gccggcgggc aagatcatct gaagggaaag atatttcgat
     1081 tgtctcatat ggggtatgcg gattcgtttg acgtgatcac tgcattggcc gcgaccgaga
     1141 tggtcctgaa aggactcggt cattccatac agctggggag tggcgttgga aaagcccaag
     1201 aaatcctatt ggcgaagtaa tcacggcgag gatgcacatg gctgcagcgg gaatgaagat
     1261 tctgatcagc gatagcttgt cgaaacaagg cgtcgaggcg cttgagaagg cagggtttac
     1321 cgtggtggtg aaatccaaaa tgccgaagga agagctattc aaagagatca aagatgccga
     1381 tggactgatc gtgcgatccg gcaccaaggt gacggcagag ctgattgctg cggcagagaa
     1441 actcaaggtc gtcggcaggg ccgggtcggg cttggacaat gtcgataccc ctgctgcgac
     1501 ccgtcgcggc atcgtcgtca tgaacacgcc gggaggcaac accgtcacca ctgccgaaca
     1561 cacgatgtcg atgatctgtg cgatgagccg gcgcattccc caagccaccg cttcggtgaa
     1621 ggcaggcaaa tgggaaaagg acaaattcat gggagtcgag ctctgcaata aagtgctcgg
     1681 catcatcggc gccggacaga tcggcagtca tctgaccaag atggcgcaag ggatcggtat
     1741 gagtgtcgtc gcgtttgacc cgtacctggc cccggaacgg gccgagcgaa tgggagtgac
     1801 gatgctggac ctcgaagaac tttttcggcg ggcggacgtt atttcggtcc atacgccgct
     1861 cacgcctgag actcgcggga ttatcaacgc acaggccatt gccaagatga aacccggcgt
     1921 gctcatcgcg aattgcgccc gtggcggcat cattaacgaa caagacctgt gcgaagccct
     1981 aaaaacaaag cgcgtcgccg ccgcggcctt cgacgtgttt gaagaagagc cggtcaagcc
     2041 ggacaatccg ctcctcgctt tggataattt catctgcacc ccgcatattg gagcccagac
     2101 gacggaggct caggagaacg tcgcgatcgg aatcgcggaa cagatcgtcg attattttac
     2161 caagggagtg gccaagggtg cggtcaatat tccgtcggtc gctccggaat tattgcctcg
     2221 cttgcaacct tttctgacat tggccgagaa gctcggtacg cttcaatctc aactcgttca
     2281 aggaggaatc gaacgagtca cggtggaata cagcggggag gtcgccacgc tctcaatcgc
     2341 gcccttgacc atcgccgtcc taaagggctt gctcactcct attatggaac atccagtgaa
     2401 ctacgtgaat gcccccattg tggcgaaaga gcgaggcatt gaagtcaaag aaattaagag
     2461 cacggatgcc ggggacttca caagcctgat acgagttcga gtcgaagccg gcaaggtgtc
     2521 acaccaagtc gccgggacgc tgtatcacaa gaaagaagct cgcgtcacgg agatcgatca
     2581 atttaaagtt gaagttgtac cagaagagca tatgttattg attcacaatg ttgatcgccc
     2641 aggtgtaatc ggtatggtcg gaaaggtcct cggagatcag ggaatcaata ttgtgcggat
     2701 gcagtgtgcc ttggaaaagc ggggagggga tgcgttattg atcatcggtt ccgacacgga
     2761 attcccaact gtggtccttg atcaaatccg ttccagctcg aatattctgt ccgtcaaggt
     2821 cgccaacctt tcgtaacgat tctctcgacc aggttattat ggcctctgct cctccgatgc
     2881 gtaggacttc ttcggggcag ttccctcttg tgcgcgaaca ctcactggtt ccggtcggga
     2941 tggccaccat tcttccggat gctgcccgcc acgttcggca tcttgaagcc gaattgctgg
     3001 cacacttcaa tcgtttcggt tacgacgaga ttatccttcc gacgttcgag tatctcgatg
     3061 tgctgatgcc gggactcgaa ccatcgctat tggagaattc gtataagatt gtggaccgga
     3121 cgaccggccg tattttgttg ttgcggcccg acgtcacggc acaaatagcc cgcacggtgg
     3181 cgatgggtat ggtgggcaca cactttccgt tgcgcttgtc gtaccgggcg accgtctttc
     3241 ggtatgaacc ggaacatgcc ggccgagatc gagaaatctt tcaggtgggc gccgagctga
     3301 ttggagctga tgatccgtcc gccgatagcg aaatcatcat gctgttgatc gagtgcctac
     3361 ggcaggtcgg attgcgctcg ttcaagattt cactggggca tgttgggttt ttcaaaggac
     3421 tgctcattcg tgcgggactc tcgtcagaag ggcagaagcg ggctgagcaa gcagcggcaa
     3481 gaaaagatgt accgaggctt ggggagatcc tgtcgcagga gcgggtgacc aaaagatcgg
     3541 ctcacagaat tctggacgcg ctggaactgt gcggacaagc cgaagttctc tcaagagggc
     3601 gagcattggc aaagggtgag aggccacttg tccaagcatt ggatcgactg gcgcatgttt
     3661 atcgtctgct gtgcgagact ggatttcaag aggctgtctt gttggattta ggagaattca
     3721 gagggttcga ctattatgac ggcatcgtct ttgatgtctt taccgacggt attggagtgg
     3781 agttaggcgg cggtggccgc tatgatcact tgatcgggcg attcggtcgg aaccttcctt
     3841 cgaccggatt tgccctgagt gtggaccggc tcttccgagg gttgaatcat tccgatagaa
     3901 cgaagtctgc ggggccggag gtcttggccg tcggtccgct aggattgtcg acgcgactgg
     3961 tttcggtcgc tcaacaactt cgccaagcgg ggatacgaac ggttcaacgg atcgtgaatc
     4021 catcgggacg ggacttgatt ggcgccgcgg tcaaggccgg ccttgaagca gaaatcgata
     4081 ccattattgt cgtcggcacg gacagtccct cacctgagca agtcgttgtg ctccatcagg
     4141 gcaacatgag catggccggt cgatcccacg cctcgcctgc tgttcgcaag aagaccgtgt
     4201 cgatcacaga ccttattgca agtctgcgtg tcgatctctc taaaggggcg gtatgaaatc
     4261 cattgggatg gtggatctct cccaaccgaa actacctccg cagaacttgg aggcggagca
     4321 gtccgtactc ggcgccatcc tcctcgataa cgccgccatg ccaaaggcga tggaactgct
     4381 ggtggaggag gacttctatc ggacgtcgca caagaaagtc tatcaagcca tgcttgagct
     4441 gtccgacacc ggagaggtga tcgatcagat cacgttgacc gagcgattga aatcgcgcgg
     4501 tgagctcgaa accatcggag gcgcggccta tctcgccgaa ctcgtccaga tcgttcccag
     4561 ttccgccaat attcggtatc actgtaagat cgtccgggac aaggcagtgg ccc
//
LOCUS       DASTWE010000036         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_614844, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000036 DASTWE010000000
VERSION     DASTWE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614844"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..983
                     /locus_tag="VFT30_00410"
     CDS             498..983
                     /locus_tag="VFT30_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5091111.1"
                     /translation="MRLLLAVDDSENAHRAVRYVGSLLGHTPDVTVTLFHVLKPMPRV
                     LLEHGGSENPAVEEQLSARLREDRETWIRKEREAECSVLRKACETLEQCGFDTSCVSL
                     KFGHEDDVAANILEEARRGQHDTIAVGRRGISRITRMFGGGVTDRLLRDAKGFAIWII
                     E"
BASE COUNT          309 a          307 c          323 g          286 t
ORIGIN      
        1 cgtcgcacgc acagttattt tacctagtag aacgtttctt tacgccgctt cctttccacg
       61 atgcccttac gtctgcgcat tggctgcttt ttcgatctct gaaccaatct caccgctgag
      121 catggcacca taccggctcc aataaagcca caacatgtct agttttaggg atttccccag
      181 tactacacga catttgaatg cgatatcgta gaattaggac aagcaatgga aacattgtga
      241 atgcatagcg gaaacgttat ccaagcatga tgtaaaaatt gcatgttcga aggcttcgcc
      301 cttgaaaaag gacggagctt tttttatgga ctcagagtgc agttccataa aggacgctaa
      361 gaatcgccgg tcatgcgtga ctcatcgctg cctatactca aagcagccga acgactgttg
      421 acgtatatgc ctgggcacgc tcatcctgtt tgcggaacag gtaagacccc ttagattctg
      481 gaaggtctga aaccgagatg cgtctcctac tggccgtcga cgattcggaa aatgcccacc
      541 gtgccgtccg gtacgtcgga tcattactgg gacacactcc ggacgtgacc gtcacgctct
      601 ttcatgtgtt gaagccgatg ccacgggttc tgttggagca tggaggatcg gagaatcctg
      661 ctgtcgagga acaattgagc gcccgcctgc gggaagatcg ggaaacatgg atccgaaagg
      721 aacgggaagc cgaatgctcg gtcctgagga aggcctgtga aacactggag caatgcggtt
      781 tcgatacgag ttgcgtgtcc ttgaagttcg gccacgagga cgatgtcgcc gcaaatattc
      841 tcgaagaggc gcgaagagga caacacgaca cgatagcggt gggccgccgt ggaatatccc
      901 ggatcacacg gatgttcggc ggcggcgtaa ccgatcgatt actacgcgac gcgaaggggt
      961 ttgcgatctg gatcatcgaa taaacgccac aaaagttgct gaacgctgcc gtcactagaa
     1021 gcagcgacag gtccatcaaa tcagccgggc cattgttcac aaagccgatg agcagtggtc
     1081 tgtcctttaa atcagtcata agccaaatga gagcgtcgtc gcatcgcttc ttcgatggca
     1141 tttaaggact ttccttcagt tgtgtagaca ctttgttctt tcgtgaggca ttctgaaaac
     1201 cgtattgtcg aagctgcgca cagaa
//
LOCUS       DASTWE010000037         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1002741, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000037 DASTWE010000000
VERSION     DASTWE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002741"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFT30_00415"
     CDS             complement(<1..369)
                     /locus_tag="VFT30_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU5091112.1"
                     /translation="MTKIKICGIKTVDDALAAMDAGADLLGFNFYPKSPRYISVGGCR
                     DVMSVMRKYGQITYVGVFVNASVAEIRATMETASLSLAQLHGDETPEIVRALDEKAFK
                     AFRGVPDRLNGFAREDAPALL"
     gene            complement(441..>1020)
                     /locus_tag="VFT30_00420"
     CDS             complement(441..>1020)
                     /locus_tag="VFT30_00420"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU5091113.1"
                     /translation="KFFLGKLATLRELRFERKSELPLLRKDFIIDEVQLYESRANGAD
                     AVLLIAAALTDDQQFSGLHALALDLGLTPLVEVHDEAETERALKLKDVRLIGINNRNL
                     ATFEVSLEPTERIRPMIPDGITVVAESGIFTAGDVERLAQAKVDAILVGEALVTSEDI
                     GAKVRELSGMSSRAEVETEVKPSPVKSWTPIE"
BASE COUNT          227 a          276 c          275 g          242 t
ORIGIN      
        1 caggagcgca ggagcatcct cacgggcgaa accgttcaat cgatcgggaa cgccgcgaaa
       61 tgccttgaat gctttttcat caagagctct gactatctcg ggagtttcat cgccgtgaag
      121 ctgtgccagg gacaacgaag ctgtttccat cgtcgcgcgg atctcggcaa ccgatgcatt
      181 cacaaacacg cccacataag tgatctgacc gtatttacgc atcaccgaca tgacatcccg
      241 acatcccccg acgctgatat agcgagggct tttggggtag aagttgaagc cgagcaggtc
      301 ggcgccggca tccatcgcgg ccagggcatc gtcgactgtt ttgatcccgc agatttttat
      361 tttggtcata tctattaatc catgtcattg cgatgaggag cgtagcgacg aggaagcaat
      421 ctcctgggat atagcaaacc ttattcgatc ggagtccacg actttacagg ggatggtttc
      481 acttctgtct cgacttccgc tcgcgatgac attcccgaaa gttctctgac ctttgcgcca
      541 atatcctctg aggtcaccaa cgcttcgccg acgaggatcg catccacctt cgcctgtgca
      601 agtcgctcca cgtcgcctgc agtaaatata cctgactcag ccacaacggt aatgccatcg
      661 gggatcatgg ggcggatccg ttctgttggt tccagagaaa cctcgaaggt cgcgagatta
      721 cgattgttga tcccgatcag gcggacatcc ttcaatttca gggctcgttc ggtctcggct
      781 tcgtcatgca cttccacaag cggggtcagt cccaggtcaa gtgccagggc atgtagaccg
      841 gagaattgtt gatcgtccgt caaggcggcg gcgatgagga ggacagcgtc cgcgccgttg
      901 gctctcgatt cgtagagctg gacttcgtca atgatgaagt cttttcgaag caggggcaat
      961 tctgatttac gttcaaagcg cagctcgcga agggtagcga gtttccccag gaagaatttt
//
LOCUS       DASTWE010000038         1392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_582558, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000038 DASTWE010000000
VERSION     DASTWE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582558"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..>1392)
                     /locus_tag="VFT30_00425"
     CDS             complement(102..>1392)
                     /locus_tag="VFT30_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEU5091114.1"
                     /translation="YIAAAEPRPRFGVTYKLPLIYAGNKEAQPQVKKILEDKSALVMT
                     ENIRPVLERENLAPARNKIHDLFLEHVMQQAPGYKKLIDMAGAPIMPTPAAVGLIMET
                     IAKREHLNLIGVDIGGATTDVFSVFDGAFNRTVSANLGMSYSVSNVLAEAGLPNIMRW
                     VPFTIDEQKLRNRIKNKMIRPTTIPQTLDELQIEQAIAREALRLALIHHKSLATGLKG
                     VQQERTISDVFEQQTSGKTLIDMLKLDLIVGSGGILSHAPRRIQSMLMMVDAYEPMGC
                     TRLSVDSIFMMPHLGVLSTINEKAATEVFVRDCMIYLGTCVAPIGQGKDGEVCADYEI
                     TWPDGKTMKERLKFGDLRLFPLESGKQATIKVQPAKQVNMGAGAGTAITKEVLGGVVG
                     LLLDGRGRPLRLPADQQDRVAALTKWFNAVGLYPTAK"
BASE COUNT          284 a          412 c          357 g          339 t
ORIGIN      
        1 tgagaccggg tgtgtaggag tgagccactt tgttcaccag gactgagcaa cgaggactgt
       61 ggactgagtg aatgcggctt ctcttagcac ctcagtcctc attacttagc agtcgggtac
      121 aaaccaactg cgttgaacca tttcgtcaaa gctgccacgc gatcttgttg gtcagccggg
      181 agtcgaagcg gccgaccacg gccatcgagc aaaagtccaa cgacgcctcc aaggacttcc
      241 tttgtgatcg ccgtccctgc accagcaccc atgttgacct gtttggcagg ctgaacctta
      301 atcgttgcct gtttaccgga ctccaatgga aagagccgta aatcgccgaa tttcaatcgc
      361 tctttcatgg tcttcccatc cggccacgta atttcatagt cggcacacac ttcgccgtct
      421 tttccctgtc caatcggggc cacacaggtc ccgagataaa tcatgcaatc tcggacaaag
      481 acctcggttg ccgccttctc attgatcgtg gagagcactc cgagatgcgg catcatgaag
      541 atgctgtcga cagacaatct cgtgcagccc atcggctcat aggcatcgac catcatcaac
      601 atcgattgga tccggcgcgg cgcatgcgag aggatgccgc cgcttccgac gatcagatcc
      661 agcttcaaca tgtcgatgag cgtcttgccg gaggtttgct gctcgaagac atccgaaatc
      721 gtccgctcct gctgtacacc ctttaatcca gtggccagcg acttgtgatg aatcaacgcc
      781 agtcgcaagg cttcccgcgc gatcgcctgc tcgatttgca gctcgtcgag cgtctgagga
      841 atggtcgtgg ggcggatcat cttattctta atgcggttgc gcaacttctg ctcatcaatc
      901 gtgaagggca cccatctcat gatgttgggc agtcccgctt cagccagcac attcgacacg
      961 ctgtatgaca tgccgagatt cgcactcact gtccggttga atgctccatc aaagacggag
     1021 aacacgtccg tcgtcgctcc cccgatatcc acaccgatca ggttcagatg ctcacgtttg
     1081 gcgatcgtct ccatgatcag tccgaccgct gctggagtcg gcatgatggg cgctcctgcc
     1141 atatcgatga gcttcttgta gccgggcgcc tgctgcatga cgtgttcgag gaacaggtca
     1201 tgaattttgt tccgcgccgg cgccaaattc tctcgttcca ggaccggtcg tatattctcc
     1261 gtcatgacca gcgccgattt gtcttccagt atctttttga cttgcggttg ggcttccttg
     1321 ttgcccgcat agatcagcgg caacttgtat gtcacgccga accgtggtcg tggttccgcc
     1381 gccgcgatat at
//
LOCUS       DASTWE010000039         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_905818, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000039 DASTWE010000000
VERSION     DASTWE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905818"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..859)
                     /locus_tag="VFT30_00430"
     CDS             complement(305..859)
                     /locus_tag="VFT30_00430"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU5091115.1"
                     /translation="MNNNLNKTTDQEHTRAEMDKLIASLDLPELNKEFMRARWLELVI
                     WMDGKAQDSVWWYRRLRLATIIGGVIVPALVSLNIGDDVAPLIKTLTFIVSLVVALSA
                     AVEEFFRYGERWRHYRRMTESLKSEGWHFLQLSGTYANHTHVQAYPVFATRVEELSRE
                     EVETYIAQVFKEKKEADDKTPSAS"
     gene            complement(1143..1796)
                     /locus_tag="VFT30_00435"
     CDS             complement(1143..1796)
                     /locus_tag="VFT30_00435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5091116.1"
                     /translation="MNRLVPFDIEAYAEIHSMPESSVCRALREETHRIMEHSQMLVGP
                     LEGAFLKMTTLLVGAKQVLEIGMFTGYSALCFAEAMPDDGIVITCDIDEKAAAVARRY
                     IAQAPFGKKISIRMGPALDTMRTLCGPFDLIFIDADKTNYLNYYRRSLDLLAPNGVIL
                     IDNVLWSGEVLKQPPPDESTAVIQELNRTVSADPCVTAVLVTIRDGILLVRRAQRRT"
     gene            1904..>2452
                     /locus_tag="VFT30_00440"
     CDS             1904..>2452
                     /locus_tag="VFT30_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEU5091117.1"
                     /translation="MKTLATLEPLTARLLEIQRINSAASVLSWDQETYMPAGGGEARA
                     EQIAVLQGLAHQKLVSPDVQHLLAQWVDPATGQAADQPGETWDEPSRSLLREVWRDFS
                     RAKKLPSDFVVRLSRECSLAQQVWAEAKEQNKFVMFLPNLRTVLQLKREEAEYLGYHD
                     SPYNALLDVYEPGATIAGLQPMF"
BASE COUNT          582 a          680 c          634 g          556 t
ORIGIN      
        1 gatacaggac tgtatcgaaa ttagtacggt aaggtcttgt ggatattcag aagtcatcaa
       61 gcccgacttc aagaccagga agagatcaac aattccactt gtcgaatgtc gtgaggctca
      121 cgcttccttc atctgtccga agacttcgaa accagcagct tctaatgcca gtttatcagt
      181 tcgacatgga tgcaattgaa atcgatctcg atgccttgat agtccttcat tgaagggcat
      241 cgcgacaagc catggaggca cgcaacgttc ttctcaatag acagacccgc ccaaccctgc
      301 aacttcacga cgcagagggg gtcttgtcat ctgcctcctt tttttctttg aacacctgcg
      361 cgatgtaggt ctcaacctct tcacgactga gctcttccac tcgagtcgcg aagacaggat
      421 aggcctgaac atgcgtgtgg ttggcatagg ttccgctcag ctgtagaaaa tgccagcctt
      481 cactcttgag cgactcggtc atccgacggt agtgtcgcca acgctcacca tagcgaaaga
      541 actcctccac ggcagcgctc aacgccacca cgaggctgac gatgaacgtg agcgttttta
      601 tcagaggtgc gacgtcatcg ccgatattca ggctaaccaa cgccggtacg atcacaccgc
      661 cgatgatcgt cgctaggcgt agcctgcgat accaccacac ggagtcctgc gccttcccat
      721 ccatccaaat gacaagttcc aaccatcgcg cccgcatgaa ctccttgttc aattccggca
      781 aatccaggga tgcgatgagc ttgtccatct ctgcccgcgt gtgctcttgg tcagtcgtct
      841 tattcagatt attgttcata ggctcctccg ccgagtcgtt cacgacgaga gaaagatcac
      901 ttatttttca aacgcaaaga atagcccctt tattcatttc gcttgagcaa tccctccaag
      961 caacactgct ctcatatctg caattttgca caagccgccc atgaaccaat aaaaatgggc
     1021 tatgcttgag tgaggtagga agcggcgatg aaggtgtgtc gccataggcg agatatcgaa
     1081 gtagatacga tgtgtatcaa tacagatacc ataagttgga gggataacgt aaccaatagt
     1141 tatcatgttc tacgctgtgc tcttctcaca agaagaatcc catcgcggat cgtgaccagt
     1201 acggcggtca cgcagggatc ggctgaaacg gtacgattga gttcttgaat gacggcggtg
     1261 gactcgtcag gcggtggctg tttcaagact tcgccgctcc acagcacgtt gtcgatcaag
     1321 atcaccccat tgggagcgag cagatcgagc gaacggcgat aataattgag gtaattggtt
     1381 ttgtctgcat cgatgaagat caggtcaaag ggaccgcaca gtgtccgcat ggtatcaaga
     1441 gccggtccca tgcggatgct gatctttttc ccaaatggtg cttgtgcgat atagcgcctg
     1501 gccactgccg ccgctttctc atcgatatcg caggtaatga cgattccgtc gtcgggcatg
     1561 gcttcagcga agcagagggc actatagccg gtaaacatcc cgatttcgag tacctgcttc
     1621 gctcctacca gcagcgtcgt cattttcagg aatgcccctt ccaagggacc caccagcatc
     1681 tgggaatgct ccataatccg atgggtctct tcgcgcagtg cgcggcagac cgacgactcc
     1741 ggcatggaat ggatttcggc gtacgcttcg atgtcgaacg ggaccaaccg attcatgaaa
     1801 atcctttcgt ggtttgaaaa agagcgccgc cctgcgtaca ctgcggccac actgtgttcg
     1861 tcccttccga tcttagcata gccaaaatga gaaggaggct tgtttgaaaa ccctcgcgac
     1921 gttagaaccc ctcaccgcca ggctgctcga aattcaacgc atcaatagtg ccgcctcggt
     1981 cctttcgtgg gaccaggaaa cctacatgcc ggccggcgga ggagaagcga gggccgagca
     2041 gatcgccgtg cttcagggcc ttgcccatca gaagttggtc tcgccggacg ttcagcatct
     2101 cttggctcaa tgggtcgatc ctgcaaccgg gcaagcagcc gaccaacccg gagaaacctg
     2161 ggatgaaccg tcgcgctcgc tcttgcgcga agtgtggagg gatttcagtc gcgcgaagaa
     2221 gctgccatcg gatttcgtcg tgaggctgag ccgcgaatgc tcgctcgccc agcaagtgtg
     2281 ggccgaggcg aaagaacaaa ataaattcgt gatgttcctg ccgaacctcc ggacggtgct
     2341 gcagctcaag cgtgaagaag ccgagtacct cgggtatcac gattcgccct acaatgccct
     2401 gttggacgtc tatgaaccgg gcgcgaccat agctggtctc cagccaatgt tt
//
LOCUS       DASTWE010000040         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_614916, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000040 DASTWE010000000
VERSION     DASTWE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614916"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFT30_00445"
     CDS             complement(<1..594)
                     /locus_tag="VFT30_00445"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091118.1"
                     /translation="MISLDVTGLSEHVNMPTVGTPEDTICAIATPGGEGGIGIVRISG
                     PHAIDIASRVVRLRSKRSFQTLASHKLHLADILFPLPVPSIGSHPALETHQSTEQIVD
                     EGLVVYMKAPRSFTAEDVVEIHCHGSGLVLQRVCEACVAAGARLAQAGEFTKRAFLNG
                     RLDLSQAEAVLDTIRAKSEVGLKLAQRQLRGELAQQIH"
     gene            complement(602..2296)
                     /gene="yidC"
                     /locus_tag="VFT30_00450"
     CDS             complement(602..2296)
                     /gene="yidC"
                     /locus_tag="VFT30_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5091119.1"
                     /translation="MEKRVIVFLVLSLAIIFGYEYLLKELGLLPQPTISEPADPSTSD
                     ASSPSSTSSGSPTSNAPATKEIAPPKPSGGGGRAQEPRGEPSAIEVVEVETDLYRAKF
                     TTHGAALISWELKRYRSSSDGKSAVQLVRQEGKFAEPLTVTTDDISHSKELIEGIYRV
                     ERDFTTLDSGHPTGHMTFYYDNPATGVRLEKQLTFHADSYVVDITLKPSGLAGAVKVG
                     LGTNFGVVDWGEGFIGSVGAASLIDDKIENDMPESETERKGALKWVALQDKYFIAALI
                     PKTSASALPRKQGDKLVSTAVRLAVDSSGAPLGLQLFAGPKEFDTLQSLQIRLEDTID
                     FGWFLFGSWDMVRAVAKPIFYVLRYINDYTHNYGLTIILLTVIIKLLFVPLQYKSYTS
                     MKKMQGIQPRVAAVQEKFKDDRERLNKELIKLYRDHKVNPVGGGLPMVLQMPVFVSLF
                     NILYMTIDLRQAPLGLWITDLSVQDPYYILPIIMGISMVVMQKIQPTTMDPTQAKVML
                     MLPALMTFLFINFPAGLVLYWLTNNVLTIVQQFVTDRFFFRTPAIAPTMDEQERKK"
BASE COUNT          575 a          629 c          633 g          570 t
ORIGIN      
        1 gtggatttgc tgcgcaagtt cgccacggag ctggcgttgt gccaatttaa ggccgacttc
       61 tgacttggct ctaatagtat ccaacacagc ctcggcctga gacaaatcga ggcggccgtt
      121 caagaaggcg cgtttggtaa actctcctgc ttgtgcgaga cgagctccgg cagcgacgca
      181 ggcctcacag actcgttgca gaactaatcc acttccatgg caatgaatct caaccacgtc
      241 ttccgcggta aacgatcgag gcgccttcat atagaccacc aggccttcgt cgactatctg
      301 ctcggtcgat tgatgtgtct cgagagcagg atggctaccg attgaaggaa caggcagagg
      361 gaagagaata tcggccagat gcagtttgtg agaagcgagt gtctgaaacg atctcttgga
      421 gcgcagccga acaacgcggc tcgctatgtc gatggcatgc ggcccgctta tccgaacgat
      481 accaatacct ccctcgccgc caggagtggc aattgcacaa atggtatcct cgggtgttcc
      541 aacggtaggc atgttcacat gttcggagag accagtcaca tcgagcgaaa tcaatcgaat
      601 atcatttctt cctctcttgt tcgtccatag tcggtgctat ggcgggagtt cgaaagaaaa
      661 agcgatcggt gacgaactgt tgcacgatgg tgagcacgtt attggtcaac caatacaaca
      721 ccagaccggc aggaaaattt atgaacagga atgtcatgag cgccggaagc atcagcatga
      781 ctttcgcctg agtcgggtcc atagtcgtcg gttggatctt ttgcatgacc accatgctga
      841 ttcccatgat gatcggtaga atatagtagg gatcctgcac cgacagatcc gtaatccaca
      901 accccaacgg cgcttggcgt agatcgatcg tcatatacag aatattgaac agcgatacga
      961 acaccggcat ctgcaatacc atagggagac cgccgcctac gggattgacc ttgtgatctc
     1021 ggtatagctt gataagctcc ttgttcaagc gctcgcgatc gtccttaaat ttttcctgga
     1081 cggcagcgac cctcggctgg atgccctgca tcttcttcat cgatgtataa cttttgtatt
     1141 gcagggggac aaacaatagc ttgatgatga cggtcagcag gatgatcgtc agcccataat
     1201 tatgcgtata atcattgata tagcgcagga cgtagaagat gggtttggct acagcccgga
     1261 ccatgtccca gctaccgaaa agaaaccatc cgaaatcgat ggtatcttcg agcctaattt
     1321 gcaaactctg aagcgtgtca aactccttgg ggccggcgaa tagctggagt ccgaggggtg
     1381 ccccagacga atcgacagcc aaacgaaccg cagtggaaac gagcttgtcc ccttgtttcc
     1441 tgggcaaagc ggacgcactc gtttttggaa taagtgcggc aatgaaatac ttatcttgca
     1501 gcgcgaccca tttcaaagcg cccttgcgct cagtctcgga ttccggcatg tcgttttcaa
     1561 tcttatcgtc gatgagcgaa gccgctccga ctgaaccgat gaacccttct ccccaatcca
     1621 ccaccccaaa attcgttccc aacccgactt ttaccgcccc tgcgagaccg ctgggcttca
     1681 acgtgatgtc tacaacatag ctatcggcgt gaaaggtcag ttgtttttca agccgaacgc
     1741 cggtcgccgg attatcataa tagaaggtca tatggcctgt gggatggccg gagtctaagg
     1801 tcgtaaaatc tcgctcgact ctgtagatcc cttcaatcaa ttcctttgaa tgagagatgt
     1861 catccgttgt gaccgttagg ggctcggcga acttgccttc ttgtcgcacg agttgcaccg
     1921 ccgacttccc atccgaactg cttcgatagc gtttgagttc ccagctaatc agcgcggccc
     1981 cgtgggtcgt gaacttagcc cgatagagat ccgtttctac ctcgacgacc tctattgcgg
     2041 aaggttctcc ccgcggttct tgagcccgac cgcccccacc cgagggtttt ggcggtgcta
     2101 tttccttggt tgcgggagcg tttgaggttg gagatccaga gcttgtactc gaaggggaag
     2161 acgcatcgct tgtggaaggg tcggccggtt ccgaaatggt cggttgagga agcaggccca
     2221 gctctttgag caggtactcg tacccaaaga taatcgcaag ggagagtacc aagaacacga
     2281 tgactcgctt ttccatgaag aagctgtgct ttctttaaaa aagatgtaat cgtcgctact
     2341 tgactgggtc ttctccccca ggatggaagg gatgacattt cataagccgg aggatggtta
     2401 ggccgat
//
LOCUS       DASTWE010000041         1279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_33345, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000041 DASTWE010000000
VERSION     DASTWE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33345"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFT30_00455"
     CDS             complement(<1..665)
                     /locus_tag="VFT30_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5091120.1"
                     /translation="MAHFVHRRWRNLCSFLWVVGRTPPAMQLGISAILLFMVLLGINW
                     AYHTFYKPTELFFPVENALTKSPRETWREYGALFEAHSTAVITPELLAALAQTEGSGN
                     PVARTYWRWRLISSNPLEWYQPASTAVGMFQITDGTFQEGKRYCIHNHVVVEDGPWHN
                     FNSCWFNSFYTRVIPGHAIELTAAFLDRHVAKAVGERAATFQQKQDLAAVIHLCGAGA
                     GRD"
     gene            902..>1279
                     /locus_tag="VFT30_00460"
     CDS             902..>1279
                     /locus_tag="VFT30_00460"
                     /inference="COORDINATES: protein motif:HMM:NF021584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEU5091121.1"
                     /translation="MQRLVVLSVVLLAFLMFSSAFSQSFTDKAKKDSAVDMSDEDPAM
                     QKAMERARAGLEDFLRMAGSPPPNTDQYSVKVRVNEGDNQEYLWVSNLKARGDLWSGR
                     IENLPMIRSVKKGQSYSFAKTEIV"
BASE COUNT          295 a          336 c          385 g          263 t
ORIGIN      
        1 tagtcacgac ccgccccagc gccgcaaaga tggatgaccg cggccagatc ctgcttctgt
       61 tggaacgttg ccgcccgctc tccgaccgct ttcgctacgt gacggtcgag gaacgcggcg
      121 gtcaactcga tcgcgtgacc cgggatgacc ctggtgtaga agctgttgaa ccaacaggag
      181 ttgaagttgt gccacggtcc gtcttcgacg actacatggt tatggataca atagcgcttt
      241 ccttcttgaa acgtgccgtc ggtgatttga aacatgccga ctgccgtcga ggccggttgg
      301 taccactcga gtgggtttga tgagatgaga cgccaccgcc agtaggttct cgccacagga
      361 ttaccagagc cctcggtctg agccaacgcg gcaagcaatt ccggtgtaat gacggctgtt
      421 gaatgggcct cgaacaacgc cccgtattcc cgccaggtct cgcgagggct cttagtgaga
      481 gcattctcca ctggaaagaa caattccgtc ggtttgtaga acgtgtgata cgcccagttg
      541 atacccaaca gaaccatgaa gagaaggatg gcgctgattc ccaactgcat cgcgggtggc
      601 gttcggccga cgacccagag aaacgagcag agattccgcc accgccgatg aacgaagtgc
      661 gccatcgaca tcaatggatg agagtggcgg gagcgagaac gggtgtggtg gcgacggggc
      721 atgtcggcag actaacagaa aacagataaa ttcttcctgt ccgagttgtg tgatgggagt
      781 acgccacagt tctttgagac gctgaatgtg tgaacgatct tgcattgcgg tccggacagg
      841 ctcaataatg ggcgaacgct cacatcattt gacggtgggc tgcagactct gggaggtttg
      901 tatgcaacga ctcgtggtgc taagcgtggt cctgttggcg ttcctgatgt tttcctctgc
      961 gttctcgcaa tcattcaccg acaaggccaa gaaggacagc gccgtcgaca tgagcgacga
     1021 ggaccccgcg atgcagaaag ccatggaacg tgcccgggcg ggcttggaag attttcttcg
     1081 aatggccggg tcgccgccgc ccaataccga ccagtattcg gtcaaagtca gagtgaacga
     1141 aggcgacaat caagaatatc tgtgggtctc caatctgaaa gcacgcggag atctctggtc
     1201 ggggcggatc gagaacctgc cgatgatccg ttcagtcaag aagggacaat cgtactcgtt
     1261 cgcaaagacg gagatcgtc
//
LOCUS       DASTWE010000042         1971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_905874, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000042 DASTWE010000000
VERSION     DASTWE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905874"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFT30_00465"
     CDS             complement(<1..644)
                     /locus_tag="VFT30_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091122.1"
                     /translation="MPEYLRFQLRRLQEETGAIEFLARSFLDPTSAHALGGIRSQLRT
                     AVSHRNQDFVLQIPKDRPIRTVPSAGEYEKRNHGNRWKGPAVVGALSFKWVMRTADEA
                     TVRDALLTGNATTMIRLLDAADGSELAMWRMEIGPDDAPGACLHVQVLGEADVRPFPS
                     SLPVPRLPSLSPTPMSSLEFLLSELFQHRWHERVHRNRDQSRTWREAQKERLSS"
     gene            complement(637..1743)
                     /locus_tag="VFT30_00470"
     CDS             complement(637..1743)
                     /locus_tag="VFT30_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091123.1"
                     /translation="MTDAKSPTILVVDDELRAAKGLKKRIGSSAEVLTRTPEAVTQAD
                     LRQADLILVDFELHEEMGEVPITQPPNGLALSSVLREQIREMNPRKVTGVALYTGQLQ
                     RISGTIPDEVRGFVVARLTNLEWVFEKNANDTEITSVSLAEAIKRLPSSWPEDANEAA
                     RHLHRFLGLSRSVPFFAVAEEDIAACHPPVHELSNASHALAVVRWLAQRILPYPAFLM
                     DRIGLAARLRLTPRRLDLLLAGESRLVDALVGVAYDGALAELYGSHWWRAGIDELIFE
                     WSEGTGEAEVLQASIQNLAGRELSFIDGEVVPVIDSSYRADRVAPIESALPVRPDDWP
                     VFADDAWAERVEIEGDPRLRGLLISPDAELVSDA"
     gene            complement(1740..>1971)
                     /locus_tag="VFT30_00475"
     CDS             complement(1740..>1971)
                     /locus_tag="VFT30_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091124.1"
                     /translation="LLRVENTGVAVDLDADPDRWFRPFETTTSDIDPLLGQGMGFGLP
                     ITRDILDEYGASIEFVRPRKGYATAIAIAFPS"
BASE COUNT          440 a          598 c          546 g          387 t
ORIGIN      
        1 tagcttgaaa gacgctcctt ctgcgcctcc cgccaggtcc gactttgatc acgattgcga
       61 tgaaccctct catgccagcg gtgctgaaaa agctcggaga gcaagaactc aagagagctc
      121 atgggcgtcg gagaaagact cggaagccgc ggcactggca gcgagcttgg aaacggccgc
      181 acatctgcct ccccaaggac ttggacatgg agacaagccc caggagcatc gtcggggccg
      241 atctccattc gccacatcgc taactcactg ccgtcggcgg cgtcaagcag tcggatcatc
      301 gtcgttgcgt taccggttaa tagggcatct cgaacggtcg cttcgtccgc cgtacgcatc
      361 acccatttaa agctgagtgc cccgacgact gctgggccct tccatctatt tccatggttt
      421 cgcttctcat actcaccagc gctcggaacc gtgcggattg gtcgatcctt cgggatctga
      481 agaacgaaat cctggtttcg gtgactcact gcggtgcgta gctgagatcg aatacccccc
      541 agcgcgtggg cactggtggg gtcgaggaag cttcgggcga ggaattcgat tgctccggtc
      601 tcttcctgca gtctgcgcag ctgaaaccga aggtactcag gcatctgaga caagctcggc
      661 atctggagat atgagcaagc ccctgagacg cgggtccccc tcaatctcca cgcgttcggc
      721 ccaagcatca tctgcaaata caggccaatc gtcaggacgc accggaaggg cagactcaat
      781 cggcgcgact cggtcagccc tgtagctcga gtcgataaca ggaaccacct ctccgtcgat
      841 aaacgaaagt tccctcccgg ccagattctg aattgaggct tggagcacct ctgcttcgcc
      901 agtcccttct gaccactcga atatcagttc gtcaatcccc gccctccacc aatgggaccc
      961 gtaaagctcc gcgagcgccc cgtcatacgc aactccgacg agtgcgtcga cgagccttga
     1021 ttcaccggcc agcagcaaat cgagccgccg aggagtgaga cgcaatcgtg ccgcgagccc
     1081 aatgcgatcc atcaggaagg cgggataagg aaggattcgc tgagccaacc aacgaaccac
     1141 tgcaagcgcg tgtgacgcat ttgagagctc gtggacaggc ggatgacagg cggcaatgtc
     1201 ctcctcagcg actgcaaaga acggcacact tcggctgagt cctaaaaagc ggtgaaggtg
     1261 ccgtgcagcc tcgttggcat cctccggcca agagctaggc agccgcttga tggcttcggc
     1321 caacgacacg gacgtgatct cagtgtcatt tgcattcttc tcaaataccc actcgaggtt
     1381 ggtaagacgg gctacaacga acccacgcac ctcatccggg atcgtgccgg agatccgctg
     1441 aagctgaccg gtgtagagcg cgacgccggt tacctttcgc ggattcattt cacggatctg
     1501 ctcgcgcagc actgagctca gcgccaggcc gttgggaggc tgcgtaatag gaacctctcc
     1561 catctcctcg tgaagctcga agtccacaag aattagatcc gcctgccgta gatcggcttg
     1621 cgtcaccgcc tcgggagtgc gcgtgagtac ctccgcgcta ctcccaatcc gtttcttcaa
     1681 gccttttgcg gcacgaagct cgtcgtcgac gacgaggatg gtgggtgatt ttgcatcggt
     1741 catgacggaa acgcaatcgc aatggcagtg gcatagccct tacgtggtcg aacaaattcg
     1801 attgaggcgc cgtattcatc caggatgtcg cgagtaattg gaagtccgaa tcccatcccc
     1861 tggccgagga gaggatcaat gtcggaggta gtcgtctcga aaggccgaaa ccaacgatct
     1921 ggatcagcat ccaaatcaac cgccacgcca gtattttcta cgcgcaagag c
//
LOCUS       DASTWE010000043         1254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1584505, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000043 DASTWE010000000
VERSION     DASTWE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584505"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1254)
                     /locus_tag="VFT30_00480"
     CDS             complement(<1..>1254)
                     /locus_tag="VFT30_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEU5091125.1"
                     /translation="WFNVSPFFVFKVSTLEYDRKRSFYDDNFHVIEDANEVLLTPLHE
                     ECPMARIERDLMDRRNVFKKRSKETDYFACMDKTLKRLGNSIYGQLGMHYLTLISDRL
                     AARMVTAQGRRHLELLQKFFVDECGYVPTYGDTDSIFVSIGETDSGPALVDRFQAWLG
                     RPVPKLEYEETFQQLVMISKKTYVGLRKSGDVKLVGFFKTIPVVMASFVNSVVELALT
                     NRDVDTFKSRLIQLVTEFEVGATGVERFVHTKKMKDSLYQYSSAVNLPHIVFARALAR
                     RGLDLRSLGGQIHYVSVVKDVKSKWEGNTYELVEDAGDMTINLPAFVAPFVAKLKSLL
                     HVLYDLPLGYLPNLIEATCAGRLEKRLDRLGTIAPFCIWAWQTRGRKPKNAPNLRQFV
                     PNWNDFASGCGVEIASRRTTSESFV"
BASE COUNT          280 a          360 c          330 g          284 t
ORIGIN      
        1 cgacgaacga ttccgacgtc gtccttctag aggcgatttc gacaccgcac ccggaggcga
       61 aatcgttcca attcggtaca aactgtcgca ggttgggcgc gtttttgggt ttgcgccccc
      121 tggtctgcca cgcccagata caaaatggcg cgatggtgcc gagcctgtcg agcctctttt
      181 cgagcctgcc cgcgcaagta gcctcgatca aattcggtaa atatccgagc ggcagatcgt
      241 acagaacgtg caacaaagac ttgagtttgg ctacgaacgg ggcgacaaag gcgggtagat
      301 tgatcgtcat gtcgcccgca tcctctacga gctcgtacgt gttaccctcc catttcgact
      361 ttacgtcctt cacaaccgac acgtaatgta tctgcccacc gagcgagcga agatccaacc
      421 ctcgcctggc caatgcgcgg gcaaagacga tgtggggcag atttaccgcc gacgagtatt
      481 gatacaggct gtccttcatc tttttggtat gaacgaatcg ctcgacgccc gtagcgccca
      541 cctcgaactc ggtcactagc tggatcaatc gcgacttgaa ggtgtctacg tcgcgattgg
      601 ttagcgccaa ttcgacgacg ctgttgacga aggacgccat gacgacggga atggtcttga
      661 aaaaccccac gagctttacg tctccggact ttctgagccc gacgtacgtc ttcttggata
      721 tcatgaccag ctgctggaac gtctcttcgt actctagctt gggtacggga cgacccagcc
      781 acgcctggaa tcgatcgact agagccgggc ccgaatccgt ctcgccgatg ctcacgaaga
      841 tcgagtcggt atctccgtag gtggggacgt agccgcactc gtccacgaaa aatttttgca
      901 gcaattccaa atgtcgccgt ccctgcgccg taaccatcct ggcagccagc cgatcgctaa
      961 ttagcgtcag atagtgcata ccgagctgac cgtatatcga atttcccagc ctcttgagcg
     1021 tcttgtccat gcaggcgaaa taatcggtct ctttgctgcg cttcttgaaa acgttgcggc
     1081 ggtccatcag gtcgcgctct atccgggcca tgggacactc ttcgtgcaac ggggtaagga
     1141 gcacctcgtt ggcgtcttcg atgacgtgaa aattatcgtc gtaaaaggat ctcttccgat
     1201 cgtactctag agtcgaaacc ttgaagacga agaacggaga tacgttaaac cacg
//
LOCUS       DASTWE010000044         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_98052, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000044 DASTWE010000000
VERSION     DASTWE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98052"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFT30_00485"
     CDS             <1..358
                     /locus_tag="VFT30_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091126.1"
                     /translation="IEVQRHITEALIEYDHESIVITRPPKKVRKGDGSIAPDATEPDT
                     LLPQDLYFSGVTGDDVRQVTVNGETIMADFVLVGMPDADFEEEDLFTARNRNFRIVGV
                     HNDTRWQRKAWVVEVK"
     gene            355..912
                     /locus_tag="VFT30_00490"
     CDS             355..912
                     /locus_tag="VFT30_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091127.1"
                     /translation="MTAWSPQSKSRMIPGISGKRIPGSNPGLPRMTGFHFTKVPRLST
                     SAEFRERHRQQVRDVRGTFRGGWGFAWQGLAAIAANIEQYAEETDEGLGRACERMAED
                     MVAYAKTNAIWKDHPGEHEDARQSIQAVVIKEPDGTWSIYLGHGKNVYYGIWLEVRWG
                     GRYAIILPTVYKFAPQLGEEVKSQT"
     gene            909..>1233
                     /locus_tag="VFT30_00495"
     CDS             909..>1233
                     /locus_tag="VFT30_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091128.1"
                     /translation="MTTVPTHFVGVFIADEVLAELFVSDGEVRIYAASSLGEGEHPEM
                     PTKPYLVWNELPSTSFQEVSETSDAEARTYTFTVYDDLGVFERIDKALKQIRSLVKSM
                     APFRSM"
BASE COUNT          274 a          353 c          378 g          228 t
ORIGIN      
        1 gatcgaggtt cagcgccaca tcaccgaagc gctgatcgag tacgaccacg aatccatcgt
       61 catcacgcga cctccgaaga aggtgaggaa gggagacggt tcgatcgctc cggacgccac
      121 tgagcccgac accttgctcc ctcaggatct ctacttctcg ggggtgaccg gagacgatgt
      181 gcgtcaggtc accgtcaacg gcgagacgat catggctgac ttcgtgttgg tgggcatgcc
      241 cgatgcggac ttcgaggaag aagatctctt cacggcccga aacaggaact ttcggatcgt
      301 gggtgtgcac aacgacaccc gttggcagcg caaggcatgg gtcgtggagg tgaagtgacc
      361 gcttggagcc cgcagtcgaa gagtcggatg atcccaggga tctccggtaa gcgcatcccg
      421 ggttccaacc cgggtctgcc tcgcatgact gggttccact tcaccaaggt ccctcggctt
      481 tcgacctcag ccgagttccg agagcgtcat cgccagcaag ttcgagatgt ccgagggacg
      541 ttccgaggcg gatggggatt cgcatggcag gggcttgccg ccatcgctgc gaacatcgag
      601 cagtacgccg aagaaacaga cgaaggtctg ggcagagcat gtgagcgcat ggccgaagac
      661 atggtggcct acgccaagac caacgccatt tggaaggacc accccggaga gcacgaggac
      721 gctcgacaga gcatccaagc tgtcgtgatc aaggagccag acggaacgtg gtcgatctac
      781 ctcggccacg gcaagaacgt ctactacggc atctggctcg aagttcgatg gggcggtcgt
      841 tacgccatca tcctgccgac cgtctacaag ttcgcaccac agctcggtga ggaagtgaag
      901 agccagacat gacgacagtt cccactcact tcgtcggagt gttcatcgct gacgaagttc
      961 tcgctgagct cttcgtcagc gatggagaag ttcggatcta tgctgcatcc tccctggggg
     1021 agggtgaaca ccctgagatg cccaccaaac cgtacctggt gtggaacgag ctgccttcga
     1081 catccttcca agaggtgtcg gagacgagtg atgctgaagc tcgcacctac accttcacgg
     1141 tctacgacga tctcggtgtc ttcgagagga ttgacaaggc gctcaagcag atccgaagtc
     1201 tggtgaagag catggccccg ttcaggtcca tgg
//
LOCUS       DASTWE010000045         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_938296, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000045 DASTWE010000000
VERSION     DASTWE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938296"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1381
                     /locus_tag="VFT30_00500"
     CDS             170..1381
                     /locus_tag="VFT30_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5091129.1"
                     /translation="MAEIKSVIGTQNKKGQTYTDTWKMMNEAPRTPSFFTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGSAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTLEIAYLLNTGML
                     VWHAETAQDFFDWILKGYMVSEEPDVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFVMKSNYISYATHASWQQEIWAAVERSRKHSIHWL
                     NGLIDTENTDADIVIVASGTAVSQGREAIRLLEDEGVRCGLVKVKTLRPWPEEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIRATVPNHQRVHAGPHVCGGMTMPPEIIVSEIKTALG
                     MKTFSLAGRGS"
     gene            1438..>1624
                     /locus_tag="VFT30_00505"
     CDS             1438..>1624
                     /locus_tag="VFT30_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HEU5091130.1"
                     /translation="MSKERIQISEALYDIMPSDYQDLVKSATYGKEDRGWKDIGTSKE
                     LIEQHSLCAGCPESMAFR"
BASE COUNT          405 a          418 c          447 g          354 t
ORIGIN      
        1 acgaagacgc catgctctcg ttccgtgatg catctgttga taaaatcatg cgattggccg
       61 ataccataaa gcgattgaaa gggaccgtcc aaccacggct ggcatcagcc aataatgggg
      121 tttcgaatta accgtttgaa atccgcgtgt gtgaaaggag gctgggtcca tggcagaaat
      181 aaaatcagtt attggaacac agaataaaaa aggtcagacc tatacagata cctggaaaat
      241 gatgaatgag gctccgcgca cgccttcatt cttcacgggc agcgaagtca tcaaggaagc
      301 tctccgtcgg gcaagctgcg atgtcatgat cgcgtatccg atcacgccac agagtgaagc
      361 ggccgcattg atcggcgaat tatttgctga aggctatatc ggagactatt tccgcggcga
      421 gagcgaattt gccgttatgt cgcaatgtgc aggttccgcg tttggcggag ctcgtgtgtt
      481 cacaacaaca gccggaccag gcaccatgcg ggccatggag aacttcccga tgtgggctgg
      541 cgctcgactc ccgattcaga tgatcgtcac gtgccgtggt attaactccc ctttgtcgat
      601 tcagccggac acgctggaaa tcgcctatct gctgaacacc ggcatgctgg tgtggcatgc
      661 agagaccgct caagacttct tcgattggat tctgaagggc tacatggtgt cggaagagcc
      721 ggatgtacac cttccactcg ccttgtgctg cgatgggttc tttgtcactc acacgaaaga
      781 cgtggtgaat ctgacgcctg ccgatatgtg cctgccaccc tacgatcctt accggtctcc
      841 cgtgccttgt atggacatgg agtgtccacc cgttcgtatg atgcgcgatc catttgtcat
      901 gaagagcaac tatatcagct atgcaacaca tgccagctgg cagcaagaaa tttgggctgc
      961 tgtcgaacgc tcgcgaaaac attcgatcca ttggctgaat ggtctgatcg acacggaaaa
     1021 tacggacgca gacatcgtga tcgtagcttc aggcactgcc gtttctcagg gtcgagaagc
     1081 gatccggttg ttggaagacg aaggcgtgcg atgcggtctg gtaaaggtga aaaccttacg
     1141 cccctggccg gaagaagaaa ttcgcgaggc taccaagaac gcgaaacaca tttttgtgcc
     1201 agagttcaac gtaactgggt ggttggccaa agaaatccgg gccaccgtcc caaatcacca
     1261 gcgcgtccat gccggacccc atgtttgtgg aggcatgacg atgccaccgg agatcatcgt
     1321 gtctgaaatt aagacagccc ttgggatgaa gacattctcc ctggctggcc gtggaagctg
     1381 aactacactg actgcgaagc tgtccgctct gatgagcgac actgaggagg cctatatatg
     1441 agcaaggagc gcattcaaat atccgaagcc ctatacgaca tcatgccatc ggactaccag
     1501 gatctcgtaa aaagcgccac atacgggaag gaagaccggg gctggaaaga cattgggaca
     1561 tctaaggagc tgatcgagca acattcgctc tgcgccggct gccctgagtc gatggccttc
     1621 cgct
//
LOCUS       DASTWE010000046         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1584673, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000046 DASTWE010000000
VERSION     DASTWE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584673"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>1003
                     /locus_tag="VFT30_00510"
     CDS             118..>1003
                     /locus_tag="VFT30_00510"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5091131.1"
                     /translation="MDGIAIGETLADPASPVALPTIRVEEPTVRMTFGVNTSPFAGKE
                     GRWGTSRNLRERLFEELRTNVALRVEETESAENFLVSGRGELHLGILIETMRREGYEF
                     QVSRPEVIFHKADDGALLEPYEEVHIETSNETVGAVVEMLGARRGQMMDMQEAGQGMT
                     RMVYIAPTRGLLGFRYQFLTSTRGAGVMHTIFHGYDEMAGPMSTRTTGSLVAWEAGDT
                     TAYALKNAEERGSLFIGPGVAVYEGMVVGENARGQDMPINVCKKKHLTNIRSAGGDME
                     IRLTPPRRMSLDEAIEYLS"
BASE COUNT          236 a          265 c          297 g          205 t
ORIGIN      
        1 atgaccagag atggccgcaa tctcccggaa tcagcgaaat atctttacac attccaggga
       61 ttgaacaaaa tcgagatcga atagtccagc gcgggtgatc ttgtctcgct agctggcttg
      121 gatgggatcg ccatcggtga aacactagct gacccggcaa gccctgtcgc gctgcctacg
      181 atcagggtgg aagaaccgac cgtccgtatg acgtttgggg tgaatacctc gccctttgcc
      241 ggaaaagaag gtcgatgggg cacatcacgc aatctgcgcg agcgtctttt cgaggagctg
      301 cgtacgaatg tcgcgttacg cgtggaagaa actgaatccg ctgaaaactt ccttgtctcc
      361 ggacgcggtg aattgcacct cggtattctg atcgagacaa tgcgccgcga aggatatgaa
      421 tttcaggtct cccgccccga agtgatcttc cacaaagcag acgacggggc tctgctcgaa
      481 ccgtatgaag aagttcacat tgagaccagt aatgaaacgg tcggcgcggt ggtggaaatg
      541 ctcggcgcgc ggcgcggcca gatgatggat atgcaggagg caggtcaggg catgacccgc
      601 atggtctaca ttgctccgac gcgcggcttg ctcggcttcc gctatcagtt cctcacctcc
      661 acgcgcgggg cgggagtgat gcatacgatc ttccacggct acgatgaaat ggcaggaccg
      721 atgtccacac gcacgacagg ctcgctcgtt gcctgggaag cgggtgatac caccgcctat
      781 gcattgaaga atgcggaaga acgaggttca ttgttcatcg gaccaggggt tgcggtgtat
      841 gaaggcatgg tcgttggcga aaacgcccgc gggcaggaca tgccgatcaa cgtctgcaag
      901 aaaaagcatt tgacgaatat ccgttcagcc ggtggcgata tggaaatccg cctcactccg
      961 cccagacgta tgtctctcga tgaagcaatc gaatacctgt ctg
//
LOCUS       DASTWE010000047         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1261555, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000047 DASTWE010000000
VERSION     DASTWE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261555"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFT30_00515"
     CDS             <1..710
                     /locus_tag="VFT30_00515"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU5091132.1"
                     /translation="LATDPKTTADEYGVLCLSLMARDGCAPQQITGAIISSVVPALTE
                     TFESMVETSFGCTPITVSADMDTGLILKYSNPKEIGSDRIVNAAAAYEKFRRDLIIVD
                     FGTATTFCVVNRDGEYLGGVIAPGLTISADALFTRAAKLSKVELTRPKTVIGTDTAGS
                     IQSGLIFGYAGLVDTLIHRMEGEMGRTSYVIATGGLASVIVPEARSIQHIEPFLTLHG
                     LELLYRRARGASLKQWA"
     gene            799..1380
                     /gene="grpE"
                     /locus_tag="VFT30_00520"
     CDS             799..1380
                     /gene="grpE"
                     /locus_tag="VFT30_00520"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5091133.1"
                     /translation="MSDDQEQEKTNANTIESLEEDSPVADAGSSVQEELAGKTEECKA
                     LNDKYLRLAAEFENYKRLTQRDQREQIRFGNEQLLKELLSVVDNLERAIKAARTNGSD
                     SALVQGVELTLKQLSGILVKFGVQAVETAGQDFDPSAHQAVSYGPSNDVPANKILDEF
                     QKGYRLHDRILRAAMVSVSSGPAQPSEHDSTTN"
     gene            1422..>2220
                     /locus_tag="VFT30_00525"
     CDS             1422..>2220
                     /locus_tag="VFT30_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU5091134.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVAITD
                     KSERLVGQIAKRQAITNPENTIFSVKRLMGRKFKSREVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEISAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDISVLEIGEGVFEVKSTNG
                     DTYLGGDDFDQRVMDWLVDEFKKDQGIDLRKDRMALQRLKESAERAKI"
BASE COUNT          549 a          595 c          618 g          458 t
ORIGIN      
        1 ggttggcgac cgatccaaag accactgccg atgagtacgg tgtcctctgc ctcagtctca
       61 tggcacgaga cgggtgcgcc cctcagcaaa tcaccggtgc gatcatctcc agcgttgttc
      121 ccgccctcac ggaaacgttc gaatcgatgg ttgaaacgtc ctttggctgc acccccatca
      181 ccgtctcggc tgacatggat accggcttaa tactaaaata ctcgaacccg aaagagatcg
      241 gaagtgaccg tattgtgaat gcggctgccg cctatgagaa gtttcgccgt gatctcatca
      301 tcgtcgattt cggcacagcg acgacgtttt gcgtggtcaa ccgagatgga gagtatcttg
      361 gaggtgtgat cgcaccaggt ctgactattt ccgcggatgc cctgttcaca cgagctgcaa
      421 aactgagcaa agtcgagctc acccggccta aaaccgtcat tggaaccgat acagccggta
      481 gtattcagtc gggcctcatt ttcggctatg ccggtctcgt ggacaccctc atccatcgga
      541 tggaagggga aatggggcgc acgtcatatg tgatcgctac gggtgggtta gcctcggtga
      601 ttgtgccgga ggcgcgatcg attcaacata ttgaaccgtt tttgaccctt cacggacttg
      661 agctcctcta tcgccgtgcg cggggcgcga gtctgaagca gtgggcctag tccccttcgt
      721 acccacatca ttttattttc cctttacccg ttgacttccc tcctcctatg gatatctccc
      781 gcttgtatag gtatcaggat gagtgacgat caagagcagg aaaaaacaaa cgccaataca
      841 atcgagagct tagaggaaga ttctccagtc gcagacgcgg gctcctcagt tcaagaggaa
      901 ctggccggca agaccgaaga atgtaaagca ctcaatgaca agtatctccg gttggccgcg
      961 gaatttgaaa actataaacg gctgacacaa cgagaccagc gtgaacagat tcgattcggg
     1021 aatgaacagc ttctcaaaga actgctgtcg gttgtcgata acctggagcg ggcaatcaag
     1081 gcggctcgga cgaacggaag cgattcggcg ctcgtccagg gtgtggaact gacgctcaag
     1141 caactatccg gcatccttgt caaattcggt gtgcaggcag tcgaaacagc cggccaggac
     1201 ttcgatccaa gcgcccatca agccgtctca tacgggccat cgaacgatgt gccggctaat
     1261 aaaatattgg atgagtttca aaaagggtat cggctgcacg ataggattct gcgagcggca
     1321 atggtcagtg tatcgtcagg tccagcccaa ccaagcgaac atgactcaac gacgaactga
     1381 cctgattcag tcgtcgttgt cgataaggaa ggagtccacc aatgggtaaa gtcatcggta
     1441 tcgatctcgg gaccaccaac tcttgtgtgg cgattatgag cggcggagac cccgtcgtga
     1501 tcgccaacgc cgaagggagc cggaccacgc cttccgtcgt tgccattacc gataagagtg
     1561 aacggctggt cggtcaaatc gcgaaacggc aagcgattac caaccccgag aacaccattt
     1621 tttcggtcaa gcgcctgatg ggccgcaagt tcaagagccg cgaggtacaa gaagccatga
     1681 aacggcttcc ctacaaggtg gttgaggccg acaacggcga tgcacacgtg gaattgcgcg
     1741 gcaagcgcta tagcccgccg gaaatctcgg cgatgattct gcagaaaatg cggcagaccg
     1801 ctgaagacta cctcggtgaa aaggtcactg aggccgttgt cacggtccct gcatattttg
     1861 acgacagcca gcgccaagcg accaaagatg ccggccagat cgccggtcta aacgtcctgc
     1921 gtatcatcaa tgagccgact gccgcctccc tcgcttacgg tctggataag aagaaggatg
     1981 aacgaattgc cgtgtacgat ttgggcggcg ggaccttcga catctccgtg ctcgaaatcg
     2041 gggaaggcgt cttcgaggtg aaatccacca acggtgatac ctatctcggc ggtgatgact
     2101 tcgatcaacg cgtgatggat tggctggtcg atgagttcaa aaaagaccag ggcatcgatc
     2161 tgcggaaaga ccgaatggcg cttcaacgcc tgaaagaatc ggcggaacgg gccaagatcg
//
LOCUS       DASTWE010000048         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_195146, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000048 DASTWE010000000
VERSION     DASTWE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195146"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..499)
                     /locus_tag="VFT30_00530"
     CDS             complement(47..499)
                     /locus_tag="VFT30_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5091135.1"
                     /translation="MLEQEKTTKWELPGVPAGGLRKVGQIRGTNTLILHAGQNGMLVA
                     LELLSTHTPGAPVVNARNEFIGFISEFDVLRALEAGKNLSNLTAEDIMVRDQISVTAE
                     TTIEEAVKTMEEKRLHNLPVKKDGRVAYSVTRHDLLRAWIGLGTSGED"
     gene            complement(707..>1288)
                     /locus_tag="VFT30_00535"
     CDS             complement(707..>1288)
                     /locus_tag="VFT30_00535"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEU5091136.1"
                     /translation="RLLTPPFDQMSRDPGYIKGYVPGVRENGGQYTHAAVWTVLAFAA
                     LGEGDKAGELFRMLNPVHRISSRASAQRYKVEPYVVAGDVYAEAPHVGRGGWTWYSGA
                     AGWLYRAGVEWMLGFRLRGATLSIDPCIPRHWPGYSIRFRYHSAIYNISVENPRHVSR
                     GVTLTELDGKVSTDNMNIPLVVSGNHHIRVVLG"
BASE COUNT          309 a          383 c          332 g          264 t
ORIGIN      
        1 actccgggtt ggattaaccc ttaactcata acggaggcga ctcatctcaa tcttcgcccg
       61 aagtgccgag gcctatccag gctcgcagaa gatcatgccg cgtcacagag taggcgaccc
      121 ttccatcctt cttcacagga agattatgca ggcgcttctc ctccatcgtc ttcaccgctt
      181 cttcgatcgt ggtctcggcc gtgacggaga tttgatcacg gaccatgatg tcttccgccg
      241 tcagattaga aagattcttg ccggcttcca acgcgcggag gacatcaaat tcactgatga
      301 aaccgatgaa ctcgttgcga gcattgacca ctggcgcgcc gggagtatgc gtggacaata
      361 attccagagc cacgagcatc ccattttggc cggcgtgcaa gatgagggtg ttggtccctc
      421 tgatctgccc cacctttcgc aatcctccgg ctgggacccc gggcaattcc cacttcgtcg
      481 tcttctcttg ctcgagcatc tgactctcct ttcaatttca ccatggtgat tgaataaata
      541 ctcgtcacag aagcgaagac catgccaatt caacccgtcg agaattccag tggttgagag
      601 aaccaaaagc atcgagagca atgcgatgtg aggtgaaaca ccccaaggga ggacagtgac
      661 ggaggaatat tgctacaaag acgaatcacg ggccctccca cgtggatcat cccagcacga
      721 cgcgaatgtg gtgattgccg gagacgacga gcgggatatt catattgtct gtcgacactt
      781 tgccatccag ttccgtcaac gtcacgccgc ggcttacatg acgtgggttc tcaaccgaaa
      841 tgttatagat cgccgaatga taccggaaac gaatcgaata ccccggccag tgacgtggaa
      901 tacaaggatc aatggagagc gtggcaccac gcaagcggaa accaagcatc cattccacac
      961 cggcccggta gagccaaccc gccgcgccgc tgtaccaggt ccaaccccca cgtccaacat
     1021 gcggcgcctc ggcgtacaca tcgccggcta cgacgtaggg ctcgactttg tagcgctggg
     1081 cgctcgctcg cgaggagatc cgatgaacag gattcagcat acggaacaac tcgccggcct
     1141 tatcaccctc acccaacgcg gcgaaggcta gcacggtcca gaccgccgcg tgggtatatt
     1201 gaccaccgtt ttctcgtacg ccaggaacat agcctttgat atagccggga tctctcgaca
     1261 tctgatcaaa gggtggggtc aacagacg
//
LOCUS       DASTWE010000049         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_970820, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000049 DASTWE010000000
VERSION     DASTWE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970820"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFT30_00540"
     CDS             <1..258
                     /locus_tag="VFT30_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5091137.1"
                     /translation="PVLMTSAATVFGHLPLVLATGPGAAARNSIGMVLVTGMTVGTLF
                     TLFVVPVFYSLIAAQHQPDLEPERIALDAEDEELLTVGARS"
     gene            283..585
                     /locus_tag="VFT30_00545"
     CDS             283..585
                     /locus_tag="VFT30_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091138.1"
                     /translation="MLKITSEKNRNSVRLRLEGSLTGPWVDELEHEWRTTRPAEGIPL
                     VVDLTNVTFVGEDGKLLLKQMWREGAQLIATGCCIGHLVEEITRSQPDTFIRCGTK"
     gene            597..>1033
                     /locus_tag="VFT30_00550"
     CDS             597..>1033
                     /locus_tag="VFT30_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU5091139.1"
                     /translation="MRILSLFIGSFFLASCAVGPDYTRPDLSSPASFRMAGVEADGES
                     FANLPWWELLQDQALHDLIQTSLRENKDLKRAVASVEEFQARLFVARMDFAPKADITG
                     NAPIMGRKAQFLFPGFPNAFNYYLQGNLAWELDIWGRIRRSNEA"
BASE COUNT          224 a          286 c          294 g          229 t
ORIGIN      
        1 ccggtgctca tgacatcggc tgccactgtc ttcggccatc tccctttggt gctggcaaca
       61 ggacccggtg cagccgcccg caacagcatc ggcatggtgc tggtcaccgg aatgacggtc
      121 ggcacgctgt tcacgctgtt cgttgtcccc gtgttctact cgctgatcgc tgcgcaacac
      181 cagccggatc tggagcctga aaggatcgcg ctcgatgccg aggatgaaga actgctcacg
      241 gtgggcgcac ggagctgaag gaagattcga aagatacttg ccatgctgaa gattacttca
      301 gagaagaatc gaaattcggt gcgactcaga ctggagggca gcctgaccgg accttgggtc
      361 gatgaactcg aacatgagtg gcgaaccacc cgaccagccg aaggaatccc tcttgtcgtg
      421 gatctcacga acgtcacctt tgtgggagag gacggtaagc ttctattgaa gcagatgtgg
      481 cgagaaggcg cacaattgat tgcaacaggg tgttgtatcg gtcaccttgt ggaagaaatc
      541 actcggtcac aaccggatac tttcatccga tgcggaacga aatgagcgga gtcatcatgc
      601 gcatactcag tctcttcatt gggtcttttt tcctggcctc ctgcgccgtg gggccggact
      661 atacccggcc tgatctttcg tcaccggctt cgttcaggat ggccggcgtc gaggcagacg
      721 gtgagtcctt cgcgaatttg ccctggtggg agctgcttca ggatcaggcg cttcacgatc
      781 tgatccagac gtctctccga gagaacaagg atctcaaacg cgcagtcgcc tctgtcgaag
      841 aattccaagc gcgtctattc gtcgccagga tggactttgc cccaaaggcc gacatcacag
      901 gcaacgcacc gatcatggga cggaaagcgc aatttctgtt tcccggcttt ccgaacgcgt
      961 tcaactatta tctccaagga aatctcgctt gggaacttga catctggggt cgcattcggc
     1021 gttccaatga agc
//
LOCUS       DASTWE010000050         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1132339, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000050 DASTWE010000000
VERSION     DASTWE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132339"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..>1169)
                     /locus_tag="VFT30_00555"
     CDS             complement(364..>1169)
                     /locus_tag="VFT30_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091140.1"
                     /translation="TVAFTQALLAVAVVGLVADRATTWMTPERTPSPVVQTASLPAAP
                     PAEMARLEQQLEELARMQAKVGEALGSQQNGMAQLHQAVAKLSSTQESVASSLVMVKE
                     EVREEVEKRLEAVGRETDRTTRHLASKGRVEQEQLEAAIHTLTADNDRLSKEVAGLEE
                     HNQALKAKLQTAVASTSKAVDPGPERLLKMADSLQATQQAPFLFWVTFSEGTSQERID
                     QWVHEMKGHKGALNEGWQEVRIVPPAVPPDRFLEQIRGDKIVKAARIGQ"
BASE COUNT          236 a          360 c          277 g          296 t
ORIGIN      
        1 accttcgatg actattgcta tctattcgtt gccctattac tagtttctcc cttgttgatg
       61 gacttcatgc tacccactcc atctcgctcg gctcaacgga caaaacatct atgaccgagc
      121 caaaatatgt ccagccgagg acgcttgact ggacattgag cctcatgatt ggcactcgtc
      181 taagaaatga aaaaccgcgc ggctgaccga catgattcga acatttttgc ccgagaacaa
      241 cgtcaacata ccccttcctc tccggatgaa actcgcccaa ggcctctctt cttcgtatgg
      301 atttcggacg tatcgaatag gaagcgacga tgcgtagcta agaactctgt tccggagata
      361 ctcctactga ccgattcgtg cggcttttac gattttgtct cctctgatct gttccaagaa
      421 acgatcgggc gggaccgccg gagggacaat ccgcacttcc tgccagcctt cgttgagcgc
      481 tcccttatga cccttcattt catgtaccca ttggtcgatc ctctcttgac tcgtcccttc
      541 gctgaacgtg acccagaaga ggaacggcgc ctgctgagta gcctggaggc tgtcagccat
      601 tttcaagaga cgctcaggtc ctggatcgac agctttcgat gtacttgcaa cagctgtctg
      661 cagcttggcc tttagggctt ggttgtgctc ttccaaccca gccacttcct ttgacaaccg
      721 atcgttatct gcggtgaggg tatgaatggc ggcttcaagc tgttcctgct cgacccgccc
      781 tttgctcgca agatgtcgcg tcgtgcggtc ggtctcacga cccactgctt ccagccgttt
      841 ctcaacctct tccctgacct cctccttgac catcaccaag ctggacgcca cggattcctg
      901 ggtcgatgac aatttggcca cggcttgatg gagctgcgcc atgccattct gttgagaccc
      961 gagagcttct cctaccttcg cctgcatccg tgccaactcc tccagctgct gttcaagtct
     1021 tgccatttct gccggaggag ctgcaggaag actcgctgtt tggacgaccg gagacggcgt
     1081 acgctccggc gtcatccatg tcgtcgcacg gtcagcgacc aatccaacga cggcaaccgc
     1141 caataaagct tgcgtaaagg cgaccgttc
//
LOCUS       DASTWE010000051         1844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1584827, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000051 DASTWE010000000
VERSION     DASTWE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584827"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFT30_00560"
     CDS             complement(<1..599)
                     /locus_tag="VFT30_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5091141.1"
                     /translation="MDRKQTIPVRRGFVSCLILASTLSSVLAFAFLAHALDTPPLTGR
                     IVDLAHVLPSSTVESLTSQLAAHETQSSNQVAVLIIPSLEGEPLEEFSHRVATSWKLG
                     HKGTDNGVLLVVAIRERKVRIEVGYGLEGVLTDARSAQIIRNEIVPRFRAGDMPGGIT
                     RGIDAIVKTIDGTYQASEKAVPRQESDVIGQVVIAVIVG"
     gene            complement(633..1235)
                     /locus_tag="VFT30_00565"
     CDS             complement(633..1235)
                     /locus_tag="VFT30_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5091142.1"
                     /translation="MGHTWWKRTLIAVTMISALSVAGCGYNDLQGLDEDTKAAWSEVI
                     NQYQRRADLIPNLVATVKGYAAHEKETLEGVVNARAKATGMQMTPEVLKDPAAFEAFQ
                     KAQAGLTSALGRLIAIAENYPNLKADQNFRDLQSQLEGTENRITVARKRYIDRVAEYN
                     KMVRYFPTSLTAKLLLHLEERPNFTVADENAVAKPPEVKF"
     gene            1334..1639
                     /locus_tag="VFT30_00570"
     CDS             1334..1639
                     /locus_tag="VFT30_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091143.1"
                     /translation="MTRDQSIREPGGFQVRHRTLGIYQGSAIDLAFWHPSSGMPEHGL
                     CRFSTRDKAQALIDVLCSPACMDPMDRNDLTIELYDLAEHDRLITEYPLPSAWETPT"
     gene            complement(1655..>1844)
                     /locus_tag="VFT30_00575"
     CDS             complement(1655..>1844)
                     /locus_tag="VFT30_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091144.1"
                     /translation="VVAEDEPPVEVAAMVRSVSARGIAFKFLRKAQENKRLLTFIQSH
                     SGSTSSVLSKAVAPTVIS"
BASE COUNT          390 a          535 c          499 g          420 t
ORIGIN      
        1 aatccgacaa tgacagcaat gacgacctgc ccaatgacat cgctctcctg ccgaggaact
       61 gccttctcgg acgcctgata ggttccgtca atggttttta cgatcgcgtc gataccccga
      121 gttattccac cgggcatatc ccccgcccgg aaccgaggca cgatttcatt tctaatgatt
      181 tgtgccgatc gggcgtcggt caatacgccc tccaatccgt acccgacttc gatgcggacc
      241 ttgcgctccc tgatcgccac caccagcagg acgccattgt cggtgccctt gtgaccgagt
      301 ttccaggagg tcgctacgcg atgggaaaat tcttcgaggg gttccccttc gagggagggg
      361 atgatcagga cggccacttg attgctcgac tgagtttcat gggccgccag ttgagacgtc
      421 agcgattcaa ccgtggagct cggcaagaca tgggcgagat cgacgatccg ccctgtcagc
      481 ggcggggtat caagtgcatg tgcgagaaat gcgaaggcca agaccgagct caaggttgag
      541 gctaagatca aacacgagac aaatcctcgg cgaaccggga ttgtttgttt ccgatccaca
      601 gtctcaacct cgacctgaac ctcaatctgt tctcagaatt tcacttcggg cggctttgct
      661 acagcgttct catcggcgac cgtaaagttg ggacgctctt ccaggtgcag cagcagcttc
      721 gctgtgaggc tggtcggaaa atatcggacc atcttgttgt attccgcaac tcggtcgatg
      781 taccgcttac gcgccacggt aattcggttc tccgtcccct caagctggct ttggaggtcc
      841 cggaaatttt gatcggcttt caggttgggg taattttcgg caatggcaat gagccggcct
      901 agcgccgatg tcagaccggc ttgggccttt tgaaatgcct cgaatgcggc tggatctttt
      961 aatacctcgg gcgtcatttg catccccgtc gccttggctc tagcgttcac gacgccctca
     1021 agggtctctt tttcgtgtgc cgcgtatccc ttgaccgtcg cgacaagatt gggaatgaga
     1081 tcagcacgcc gctggtattg attgatcact tcgctccacg cagcctttgt atcttcatcc
     1141 aagccctgaa gatcgttgta accgcaaccg gccacagaca atgcgctgat catcgtgacg
     1201 gcgatcagag tccttttcca ccaagtatgc cccatcactt cctccttcgg cagtagagca
     1261 ggacgcagtg ttcctccccc tccggtcatg ctaccatggg cataatcatt aagatatgtc
     1321 gatcaagagg gctatgacac gggaccaatc gattcgtgaa cctggtgggt ttcaagtgcg
     1381 acaccgcaca ctgggcatct accaaggcag tgcgattgat ctcgcgttct ggcatccgtc
     1441 gtcaggcatg cccgaacacg gactctgccg attttcaaca agagacaagg cccaagcgtt
     1501 gatcgatgtg ctctgctcgc cagcctgcat ggacccgatg gatcgaaacg atctgaccat
     1561 cgagctctac gatctcgccg aacatgaccg cttgatcacc gaatatccgc tgccgtcagc
     1621 ctgggaaaca cctacctagg ccgggagatc gagactagct gatgaccgtc ggcgcgacgg
     1681 ctttagagag gacggaggaa gtggaacccg aatgtgattg gatgaaggtc agaagccgtt
     1741 tgttctcttg cgccttgcgg agaaacttga acgcgatgcc gcgcgcactc actgaccgaa
     1801 ccatggccgc cacttcgacg gggggttcat cctcggcgac gacg
//
LOCUS       DASTWE010000052         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_389105, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000052 DASTWE010000000
VERSION     DASTWE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389105"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFT30_00580"
     CDS             complement(<1..450)
                     /locus_tag="VFT30_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091145.1"
                     /translation="MHTVIFVVDDEQVIRTAIVKRLIRQGHFATGFESGELLLQELEH
                     KIPELVLLDLKMPGISGLDALKHVRQLVPSALVIMLTAYGTVQDAVEAMKLGAYDFLI
                     KTVDLEGVEAVVARAIEILKLRRRLESELDGQTNRYHLNNVEAHSRAM"
     gene            complement(454..>1039)
                     /locus_tag="VFT30_00585"
     CDS             complement(454..>1039)
                     /locus_tag="VFT30_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091146.1"
                     /translation="DPKRKEIEVILSETRRLDRIVNQIVDYARPRRPAPTRIDLNQIV
                     GEVSKMLKPRLEGKRLTIKVTISSMISEFTADRDLLRQVLLNIVDNAIDASPKGGAPI
                     EITGHELFRDEKPGLVIQVKDDGAGIPPELLPNVFQPFVTSGKKHGTGLGLAICQNIV
                     ESHEGDIYVTSEVGRGTIVGIWLPLEQRTALERV"
BASE COUNT          231 a          296 c          252 g          260 t
ORIGIN      
        1 catcgcacgg ctgtgagcct cgacgttatt cagatgataa cggttggtct gtccatccaa
       61 ttccgattcc aagcggcggc ggagcttcag aatctcgatc gcgcgggcga cgaccgcctc
      121 caccccctcc agatcgaccg ttttgatcaa gaaatcgtac gctcccaatt tcatggcttc
      181 caccgcatcc tgcaccgtcc catacgcggt caacatgatg acgagggctg agggaacaag
      241 ttgacgcacg tgtttgaggg catcaaggcc gctgatgccg ggcatcttca ggtcgagcag
      301 cacaagctca gggatcttgt gctcaagctc ttgtaacaac aactcgccgg attcaaagcc
      361 ggtagcgaaa tgcccttgtc tgatcagccg cttgacgatg gccgtgcgaa taacctgttc
      421 gtcatcgacc acaaaaatga ccgtatgcat ggatcaaacc ctttccaatg cagttctttg
      481 ctccaatggt aaccagatgc cgacgatcgt tccccttcca acttcgctcg tcacatagat
      541 gtccccctca tggctttcga cgatattctg acaaatagcc aagcccaatc ctgtcccatg
      601 tttttttccg gacgtcacga acggctgaaa cacgttcggg agcagttccg gtggaatccc
      661 ggcaccgtcg tctttgactt gaatcaccaa accgggcttt tcgtctctga ataattcatg
      721 ccctgtaatc tcgatgggag cgccgccctt gggggatgca tcaattgcgt tgtctacgat
      781 attcaagagt acttgtctga ggaggtctcg atcggcggtg aattcgctga tcatggatga
      841 aatagttacc ttgatcgtga ggcgcttacc ttccaaccga ggtttcaaca ttttcgatac
      901 ctcaccgacg atttggttga gatcgattcg tgtcggtgca ggtcgacgtg gccgtgcata
      961 gtccacaatc tgattgacga ttcgatccaa tcgccgagtc tccgacaaga tcacctcgat
     1021 ttccttacgc ttcggatcg
//
LOCUS       DASTWE010000053         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_195285, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000053 DASTWE010000000
VERSION     DASTWE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195285"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(678..>1058)
                     /locus_tag="VFT30_00590"
     CDS             complement(678..>1058)
                     /locus_tag="VFT30_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase early set domain-containing
                     protein"
                     /protein_id="HEU5091147.1"
                     /translation="EVFSPPYLFNADGTPAARPVISQINGETPTSTHAPEVHHGETFT
                     LETPEAASIAKVVLVRPMAVTHQTDSEQRVVQMTFYQSGSTEISATAPNGIHPHAMAP
                     RGYYMLFILNSNGVPSEGKFIHLH"
BASE COUNT          241 a          294 c          291 g          232 t
ORIGIN      
        1 agtaaacgac ggaaaccaac tcaccactgt cgcttcaacc aaagttgcag tttcaaactg
       61 taaggagtgt gaaagaccgc cggctgtctg tccccttctt accgttagct gtccggacga
      121 tgtcgaaacg cataagccaa tgacgtttca agcgaacgtc accggtgtcg atccggagac
      181 gaagacaaca atcacgtggt ccataacggc tggaaagatc atcagcggtc agggaacgtc
      241 gaagataacg gttaccgcct ccgaaaccga acggccagga cttacggcca ccgcatctct
      301 agatggcgca gatccgtcat gctcaacgac ggctgcgtca tgcactataa aaatacattg
      361 agaagatgat cgcgaactaa tgagcatcac gcagcctagt gcgatgaagg ttgtcaagct
      421 tggcagctct taacgggcca gacttaaccc ttcaatcgct ctctcgattg tctgtagttt
      481 caatgatggt tgtttgatcg gagtcataat agtcacccac cgccaagcgg atgaacttcg
      541 ctcgggagga agcgaacgat tgcctgagga aacaccctgc tattctgagc gaagcggggc
      601 agtttgtctt ctctgccccg caccgttctt ccaagtgctc tcctttgcgc cgaggacacc
      661 gggcttgatc cctcacctca gtgcaggtga atgaactttc cctccgatgg cacgccattg
      721 ctgttcagga tgaacagcat gtagtacccg cgtggcgcca tcgcgtgagg gtgaatgcca
      781 ttcggcgccg tcgcgctaat ctccgtagag ccgctctggt aaaaggtcat ctgcacgaca
      841 cgctgttcgc tgtcggtttg atgggtcacg gccatcggtc ggaccaacac gaccttcgcg
      901 atgctggcgg cctcaggagt ctccaaggtg aaggtctcgc catggtggac ttccggagcg
      961 tgagtggatg tgggggtttc cccgtttatc tggcttatga ccggccgggc agcaggcgtc
     1021 ccgtcggcgt tgaacaagta cggcgggcta aatacctc
//
LOCUS       DASTWE010000054         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1391030, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000054 DASTWE010000000
VERSION     DASTWE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391030"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFT30_00595"
     CDS             <1..626
                     /locus_tag="VFT30_00595"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU5091148.1"
                     /translation="QRPVRLVGAGRDRVQIFCRRSGPLYLQDVPEGWITGITFRYVGS
                     DQHSALNILNSTCIITQCRAMEGILSGVVLYGPECRVAFTDNEVCRNRESGIFVFAGA
                     QPRVADNRCIENHHFGIAVRDPGSRPELVRNLCEGNMLSGMLLFQHAEGLIVDNVCRN
                     NQHWGIVMTPDSHPNPSPSALPTMNQLEPNLIGAYSISDQPLAQIGR"
     gene            complement(643..>1557)
                     /locus_tag="VFT30_00600"
     CDS             complement(643..>1557)
                     /locus_tag="VFT30_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HEU5091149.1"
                     /translation="ILLVEDNDIDARLTQDILAEWSMEEFDVIHVTRLTDAFALLARA
                     RFDAVLLDLSLPDGYGLSTVRQMQAANPTIAIIVLSGLSDQTLALQAVQNGAQDYLVK
                     GEGQSELLARSIRYAIERKRAEERLTYLAQYDQLTGLVNRTLFRDRLIQAMARSKRLQ
                     QPLGLMLLDLDQFKPVNDTMGHNVGDQLLKAVAERLQECVREVDTVARMGGDEFTIIL
                     EGLTCEEDITLVAQRITKLLAEPFHLGEHRALIGVSIGITVYPIDDHEVDELLKHADA
                     AMYRAKQQGGSSFQFHIPDDSPSSTSLS"
BASE COUNT          352 a          409 c          428 g          368 t
ORIGIN      
        1 ctcaacgacc cgtgcgactt gtcggcgccg gccgagatag ggtccaaatc ttctgccggc
       61 gtagcgggcc gttgtatcta caagacgtac cggaaggatg gattaccggc atcacgttcc
      121 gctatgtcgg cagtgatcaa cattcggctc tcaacatcct caactccacg tgcattatta
      181 cgcagtgccg agcgatggag ggaatcttgt cgggagtggt gttgtatgga cccgaatgcc
      241 gggttgcatt caccgacaac gaagtgtgtc gcaatcggga gtcgggcatt ttcgtgtttg
      301 cgggtgctca accgcgagtg gccgataatc gctgtattga gaatcaccat tttggcatcg
      361 cggtccggga tccgggcagt cgtcctgaac ttgtgcggaa tctatgtgaa gggaacatgc
      421 tgagcggcat gctattgttc cagcacgccg aagggttgat tgtcgataat gtgtgccgca
      481 acaatcagca ttggggtatc gtcatgaccc ccgattcgca tcccaaccca tcgccatcgg
      541 cgcttcctac gatgaatcaa ctcgaaccga atcttatcgg tgcgtattca atatcagatc
      601 aaccgcttgc tcagatcggt cgatgacgac cgtagatgat cgttaactca aggaggtcga
      661 agaaggcgaa tcatcgggaa tatgaaactg aaatgagctt cccccttgct gtttggcccg
      721 atacatcgcg gcatcggcat gcttcaagag ttcatcgact tcgtgatcat cgatcgggta
      781 gacggtaata ccgatactga ccccgatcaa tgccctgtgc tccccaaggt gaaatggttc
      841 tgccaataac ttcgtgattc gctgtgcgac aagcgtgata tcctcttcgc acgtgaggcc
      901 ttcaaggata atagtgaact catctcctcc catgcgggcg accgtgtcca cttcgcgtac
      961 acactcttga agtcgttcag cgacggcctt caaaagctga tcgccgacat tgtgacccat
     1021 ggtatcgttc accggtttga attgatcgag gtccagcagc atgagaccaa gaggctgttg
     1081 aagccgtttg ctgcgcgcca tggcttggat aaggcgatcg cgaaacaagg tgcggttcac
     1141 aagacccgtc agctgatcat attgggcaag gtaggtcagc cgctcttcag cccgttttcg
     1201 ttctatggca tagcggattg agcgggccaa tagctccgat tgaccctctc ctttgacgag
     1261 ataatcctgc gccccattct ggacagcctg tagtgcaagt gtctggtcgc tgagcccgct
     1321 gagcacgatg atggcaatcg taggattcgc agcttgcatt tgacgcactg ttgaaaggcc
     1381 atagccatcc gggagtgaca gatccaagag gacggcatca aagcgtgcgc gggccaggag
     1441 tgcaaaggcg tcagtcaagc gggtcacatg gatgacatcg aattcttcca tgctccattc
     1501 agccaagatg tcttgggtca gacgcgcatc gatgtcgttg tcttcgacca atagaat
//
LOCUS       DASTWE010000055         5072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_647824, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000055 DASTWE010000000
VERSION     DASTWE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647824"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFT30_00605"
     CDS             complement(<1..641)
                     /locus_tag="VFT30_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091150.1"
                     /translation="MIECGQGAAVKSPTFQPLSLADFDISPERGFLPSDPCETLPDCP
                     VLNHLSREMPKLLSTRQVRQFIEEQPLLLPSPPSSWHEDEYRAAMRVLSFAGHAYVWE
                     TPGKPAVKLPSQLARPWYEIAQKLGRPPVLSYASYALDNWRRLDRTKPIQLDNIVLLQ
                     NFLGGLDEEWFVVVHIQIERQAGRGLDGLMRAINGASQGKNDEVLLGLQDLASA"
     gene            complement(952..2211)
                     /gene="ahcY"
                     /locus_tag="VFT30_00610"
     CDS             complement(952..2211)
                     /gene="ahcY"
                     /locus_tag="VFT30_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5091151.1"
                     /translation="MDYDVRDIKLADQGRLKVEWAEATMPVLRLIRKRFKRQQPLKGV
                     RVTACLHVTTETANLAITLKAGGADVRLCASNPLSTQDDVAAALVQHEGIPTFAIKGE
                     DNATYYRHIESAIAHRPHVTMDDGADVVSHLHSKRKELLRNVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSVVVVVGYGWC
                     GRGIAMRAKGMGADVIVTEIDPLKGLEALMDGFRVMPMEQAAPVGDFFVTVTGNIHVI
                     RGEHFAAMKDGAIVCNSGHFNVELDIPALEKLASKRRIVRPGVEQFTLKKNDHRVSLL
                     GEGRLVNLATAEGHPSSVMDMSFANQALSAEYIVKNYKQLEKKVYPVPPAIDKEIARL
                     KLAGMGVSIDTLTAEQKKYLASWEMGT"
     gene            complement(2334..3482)
                     /gene="metK"
                     /locus_tag="VFT30_00615"
     CDS             complement(2334..3482)
                     /gene="metK"
                     /locus_tag="VFT30_00615"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5091152.1"
                     /translation="MRDNFLFTSESVTEGHPDKIADQISDGILDAIIAQDKYSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYCDASWGFDFHTCSVLTAIHQQSG
                     DIAMGVDSGGAGDQGLMFGYATNETDELMPMPLVLAHRLTKRLAEVRKKKILNWVRPD
                     GKSQVTVEYKNGKPVRIDTIVVSTQHSPDVTNKQIERDIMEKVIKPVMPKGLYDPTGV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPTKVDRSASYMA
                     RYIAKNLVAAGLADKCEVQLAYAIGVADPVSVLVDTKGTEKVSVDILDKLVRKHFPMT
                     PRGIIDHLKLRRPIFRKTAAYGHFGRNEPEFTWEKTDKTKALRKDAGL"
     gene            3795..4349
                     /locus_tag="VFT30_00620"
     CDS             3795..4349
                     /locus_tag="VFT30_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5091153.1"
                     /translation="MKLHRAALVFTFMTVGALCAAPFSDAESPQPKKRPGSERGFVQV
                     GDEAPNFTLRDLTGNVMSLSQLRGKVVLLNFWATWCGPCRVEMPAMEQLYRTLPRKEF
                     EILAVSTDPQGAVVTRPFQREMGFTFPILHDSEYRVGLAYGARTIPITFMVDRRGIVR
                     QKIFGARDWDSPEARDLIHELMKS"
     gene            4352..>5072
                     /locus_tag="VFT30_00625"
     CDS             4352..>5072
                     /locus_tag="VFT30_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5091154.1"
                     /translation="MTQSIPQISLIAAFSAGLLSFVSPCVLPLVPSYISYITGLSVEQ
                     LTDASERVKFKKAIVLNSLLFILGFSSVFIAFGASASFFGQLLITHQDLIRRIGGVLI
                     IVFGLYLLGILNLKFLKMEHRYQFRNRPAGYLGSFLIGVAFAAGWTPCVGPVLGSILL
                     YASTTDSLLNGVVLLAFYSFGLGLPLFLTALGVDRFLAYFKEVRAYLWGVSTVSGVML
                     VLVGVMIYANSLTIVTSFLERY"
BASE COUNT         1026 a         1354 c         1423 g         1269 t
ORIGIN      
        1 gcagacgcca agtcttgtag tcccaataaa acctcatcgt tcttcccctg cgaggctccg
       61 ttgatcgctc gcatcagtcc gtccaggcca cgacccgcct gccgttcgat ctgaatatgg
      121 accacgacga accactcttc atccagccca ccgagaaaat tctgcagcag cacgatgttg
      181 tcgagttgaa tcggcttcgt cctatcaagc cggcgccaat tatccagcgc gtaggaggca
      241 taggaaagca ccggcgggcg gccgagcttc tgggcaattt cataccacgg tcgcgcgagt
      301 tgggaaggga gcttgacggc cggtttgcca ggtgtctccc aaacataggc gtgtccggcg
      361 aatgaaagaa cccgcattgc cgctcgatat tcgtcctcat gccaactcga tggaggggag
      421 ggaagtaata acggttgttc ctcgataaac tgccggacct gacgcgtgct caacaacttc
      481 ggcatttcac gactcagatg gttcagcacc ggacagtcgg gtagagtttc gcaaggatca
      541 gatggtagga atccacgctc aggagagata tcaaaatctg caagtgacag aggttggaat
      601 gtgggggatt tcacagcggc tccttgccca cactctatca ctcctccatg agattgcaat
      661 ccacttcggt ggcgccgatg agactgggta gtcaagacgc tacgatttct tgatcctctt
      721 cattgtgttg tcttttcgct cttccctcgg tgggcctgta gctctctgga gcgacctcgg
      781 cgaaggaatg gggaccccac cgagccgctg gtgatgtgaa agctggaact gactgaagga
      841 ctgcggtcgc tgaagcgacc gcagatgaag gaattccaga aggttcacgt cgcagaaggc
      901 cggaaggtca ttccgagagc ctgggaacga cagcgagtct caggcccgcc tctatgtccc
      961 catttcccag gatgcgagat acttcttctg ctctgccgtc aatgtgtcga tcgacacgcc
     1021 catgccggcc agcttgagcc gggcgatttc tttgtcgatg gccggaggca cgggatagac
     1081 cttcttttcg agctgtttgt aattcttgac gatatactcc gcgctgagtg cctggttggc
     1141 aaagctcatg tccatgacgc tggacgggtg cccctctgcg gtggcaaggt tgacgagccg
     1201 gccttcgccg agcaagctga cacgatgatc atttttcttg agtgtaaact gctcgacacc
     1261 gggccgaaca atccgccgct tgctggctag tttttcaagg gctgggatat cgagttccac
     1321 gttgaagtgg ccgctgttac acacaatggc gccgtccttc attgcggcga agtgttctcc
     1381 gcgaatcacg tggatgttgc cggtgacggt cacgaagaaa tcgccgactg gggcggcttg
     1441 ttccatcggc atcacgcgga atccatccat gagggcttca agacctttca atgggtctat
     1501 ctcggtcaca atcacgtcgg cccccatgcc cttggcccgc atggcgatgc cgcgtccgca
     1561 ccagccatag cccacgacga cgactactga gccgcagacc aagcggttcg tggcgcgcac
     1621 gatgccgtcc atagtggatt gtccagtgcc atagcgattg tcgaacatat gcttggtatc
     1681 ggcgtcgttg acagaaatca ccggaaactt gaggaccttc ttttcggcca tgctgcgcaa
     1741 ccggatcaca ccggtggtcg tttcctcggt gcccccaatg acgttccgca acagttcttt
     1801 tcgcttagaa tgcaggtggg agaccacgtc agcgccgtca tccatggtga catgtgggcg
     1861 gtgagcgatg gcagactcga tgtggcgata gtacgtggcg ttgtcttcgc ccttgatcgc
     1921 aaaggtcggg atgccttcat gttgcaccaa tgccgccgca acatcgtcct gggtgctgag
     1981 agggttcgag gcacagaggc ggacatcggc gccgccggct ttcaatgtga tagccaagtt
     2041 tgctgtttca gtagtcacat gcaaacaggc tgtgacgcga acgcctttta gaggctgttg
     2101 tcgtttaaag cgcttacgaa tcaatcgcaa cacaggcatc gttgcttcag cccattcgac
     2161 cttcaatcta ccttggtccg ccagcttgat atctctgaca tcgtaatcca cgaagtcctc
     2221 ctcctcgaaa agaaggctca ggttaaggtt gaggttgagc tagaaactca acctcaatcc
     2281 ctatgcctga gcctcagtta ttaaaccgta actttggcct cagcctgttg gcgttacagc
     2341 ccggcgtcct tgcgcaaggc cttcgtctta tcggtttttt cccatgtgaa ctcaggttcg
     2401 ttgcgtccga agtgtccgta cgccgccgtc ttgcggaaaa tcggccgccg gagcttgaga
     2461 tgatcgatga tgccccgggg ggtcatgggg aaatgcttgc gcacgagttt gtcgagaata
     2521 tcgaccgaca ctttttcagt gcccttggtg tcgacgagta cagataccgg atcggcgacc
     2581 ccgatcgcgt aggccagctg cacctcgcac ttgtcggcca aaccggcggc cacgagattc
     2641 ttggcaatat agcgggccat ataggaggcg gaacggtcca cctttgtggg atctttgccc
     2701 gagaaggcac cgccgccgtg gctgccatga ccaccatagg tatcgacgat aatcttccgg
     2761 cctgtgaggc ctgtgtcgcc cattggacca ccgaccacga atcgaccggt ggggttaatg
     2821 tgatgtttga cgccggttgg atcatagaga cccttgggca tcacgggctt gatgactttt
     2881 tccatgatgt cgcgctcaat ctgcttgttg gtcacatcag ggctgtgttg cgtggagacg
     2941 acgatcgtat cgatccgtac gggcttgccg tttttgtact cgaccgtcac ttgggatttg
     3001 ccgtcaggcc gcacccagtt aagaattttt ttctttcgca cttccgccag gcgcttggtc
     3061 agtcgatggg ccaaaacgag cggcatcggc atgagctcat ccgtctcatt ggtggcgtag
     3121 ccgaacatca acccctgatc gccggcccct ccggaatcta cccccatcgc gatatcaccg
     3181 gattgctggt gaatggcggt gagaaccgag caggtatgaa agtcgaaccc ccatgacgca
     3241 tcgcagtagc cgacgtcctt gatgacatct ctgataatat ccggtatttc gacgtaggcc
     3301 ttggtggaaa tttctcccgc tacaaaagcg ataccggtgg tcaggatcgt ttcgcaggcg
     3361 acgcgggaat atttatcttg agcaatgatg gcgtccaaga tgccgtcgga aatctggtcg
     3421 gcgatcttat ccgggtgtcc ttcagtaacc gattctgaag taaacaagaa attatctcgc
     3481 atatgcccct cgtgggttca ggttatggaa tatcagcgtg ctgtgtctgc tgattatgaa
     3541 tgtgacgaat ggtgccgagt gatagaccac gcacaaactt cggtaattct agagagatca
     3601 atcggctttg tccatcactt caatcgtagg gtgaggaaaa tttgtcactc gctgttcgct
     3661 tgactcaggt ttttgcgcac ttgtaacata ccttcgttgc aagagcatct gctgatagtt
     3721 gagtgagaag cttgatcgta tcgtcgacgg ctatttcgac ggcaggctaa gactgtgtct
     3781 acctgtgtat tggcatgaaa cttcatcgag cggctttggt gttcaccttc atgacagtcg
     3841 gtgcgttgtg cgcggcaccg ttctccgacg cagaatcacc ccaaccgaag aaacggcctg
     3901 gctccgaacg tgggtttgtc caagtcggtg acgaagcgcc gaatttcacg ctgcgcgacc
     3961 tgaccggtaa tgtcatgagc ttgtcccagc tcagggggaa ggtcgtctta ctcaatttct
     4021 gggcgacatg gtgcgggccg tgccgtgtcg agatgccggc tatggaacag ctgtatagaa
     4081 cgttgccgcg aaaggagttc gaaatcctgg cggtgtcaac agatccacag ggcgcggtgg
     4141 tcacccgtcc gttccagcga gaaatggggt tcacatttcc cattcttcat gactcggaat
     4201 accgcgtggg cctggcgtac ggggcgcgta cgattccgat cacgttcatg gtcgatcgcc
     4261 gaggcatcgt acgacaaaag attttcggtg ctcgcgattg ggattcaccg gaggcccgtg
     4321 acttgattca tgaactgatg aagtcgtaac catgacacaa tccatcccac agatttcgct
     4381 cattgccgcc ttttcggcgg ggctcctttc gtttgtgtcg ccatgcgtgt tgccgttggt
     4441 gccgagctat atttcctaca ttaccggcct gtcggtcgag cagctgacag acgcgtccga
     4501 acgggtgaag ttcaagaagg caatcgtctt gaattcattg ttgtttatcc tcggattttc
     4561 atcggtcttc attgcgttcg gtgcctcggc cagcttcttc ggtcagctct tgatcaccca
     4621 tcaagacttg attcgccgca ttggtggtgt gttgatcatc gtgttcgggc tgtatctgct
     4681 cggaattcta aatctcaagt ttttaaaaat ggagcatcgg tatcagtttc gtaataggcc
     4741 ggccgggtac ttggggtcgt tcttgattgg tgtcgcgttt gctgccggtt ggactccctg
     4801 tgtcgggccg gtgttggggt ccattcttct ctatgccagt acgacggact ctcttcttaa
     4861 tggagtcgtg ttactggcat tttattcgtt cggattgggt ttgccgctgt tcctcacggc
     4921 attgggcgtc gatcggttct tagcctattt caaggaagtg cgagcctatc tgtggggcgt
     4981 ttcgacggtg agtggcgtca tgttggttct tgtcggtgtg atgatctacg ccaattcact
     5041 cacgatagtg acaagtttct tagagcgata cg
//
LOCUS       DASTWE010000056         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1261800, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000056 DASTWE010000000
VERSION     DASTWE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261800"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..851
                     /locus_tag="VFT30_00630"
     CDS             348..851
                     /locus_tag="VFT30_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091155.1"
                     /translation="MAPKSSLAASSKAWLELLQQPTVPGSRSWYEAVFRAPVLAKTNT
                     SLALTADHLVRDLLDREMLIQQLRVSKGDNSETVEALQRELADRDHKIEALLSKKDST
                     RATTDPASIQNMQKQLAERDRKIEELSTQLEALKRIDQEMREKVRPIRPPLTTVPVPV
                     PVPDTTP"
     gene            878..2296
                     /locus_tag="VFT30_00635"
     CDS             878..2296
                     /locus_tag="VFT30_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5091156.1"
                     /translation="MEQENILVVDDDEGLLHLLKMRLSAMGFSVTPCTTGQDAVGEAK
                     KTMFNLAITDLRLRGEDGLDVTEELLRSHPGLPVIILTAHGSIPNAVEAMQRGAFGYL
                     TKPFDDKELKATIEKALAQQRMSQEIQRLKSLVKELYGLENVVARSPAMQRLFQQIAQ
                     VADSDATILLFGETGTGKEVMARVIHTNSRRGKGPFVALNCAAIPETLFESELFGHVK
                     GAFTSALGAKRGLFQMANGGTLFLDEIGEMPLSMQVKLLRAVQEREIREVGSETSVKV
                     DVRIIAATNKDLGEAVKNGTFRNDLYYRISVVPLFIPPLRDRRDDIPLLAQHFLKLSV
                     KRANKEVKGFTPAALHRLMINPWPGNVRELENAVEKAVVMSRQDMLTPDLLPAVSVSA
                     DSPLKPLTEAKEEFERTYLKNVLQLTGGNISRAAQFAGRYRADFYKMLRKYGLHPSTT
                     KGRPDSDMEEFESEANLTEAER"
     gene            2500..>2897
                     /locus_tag="VFT30_00640"
     CDS             2500..>2897
                     /locus_tag="VFT30_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091157.1"
                     /translation="MMREGKSMIVPAGALIMAITVALSPSLVLGEGGARRDVVRQAGE
                     LAAAENAAPSSLSKNATILNRDGQVVRIGNNGWMCLPDDPSTAGTDSICMNESWRNFM
                     EALKNKKKPTYTQVGIAYMLQGDRPVSNTDP"
BASE COUNT          705 a          695 c          865 g          632 t
ORIGIN      
        1 ggggcgactg tacggtttat cctacggttg gcaaaaccgg aaaaggtagg gcaaacgtga
       61 caagaaaagc atcatctctg ataagagacc ctatcatcat cagcatcagt tctatcttgt
      121 tggctggatg tgcggcttgg acaacgtcac cacccggttc tcatccctat ttttccgcag
      181 agcctcgaga agtcacagtt tttcaaatgc ttgcaaagaa acaagagagc ttagccgcaa
      241 aatgtggtga atcgagctcc tgcgaccacc tgtactttac gcgcgggttg ctaggactct
      301 acgaaagtcg cgaagttgca gagaaatatt ttggaaaggt tttagccatg gctcccaaaa
      361 gctcattggc tgcgtcaagc aaagcctggc ttgaattgct acaacaacct accgttcctg
      421 gaagcagatc atggtatgaa gccgtattta gagcaccagt ccttgcgaag accaatacat
      481 cgttagcatt gaccgccgat catctggtgc gggacttgct tgatcgggag atgctcattc
      541 aacaacttcg tgtatccaag ggcgacaatt ccgagacggt tgaggctttg cagcgtgagc
      601 tggcggatcg agatcacaag atagaggcac tgttgtcgaa gaaggattcg accagggcca
      661 cgactgatcc tgcgtcgatt caaaatatgc aaaaacagct tgccgagcgt gacagaaaga
      721 ttgaggagct ttccacgcaa ctggaggctt tgaaacgaat cgatcaggag atgcgggaaa
      781 aggtgcgccc gatacgtccg ccattgacta ctgttccggt tccggttccc gtccccgata
      841 cgacacccta acgaacatta gaagggatta tgatcttatg gagcaagaaa acattctcgt
      901 agttgacgac gacgaggggc tgttgcattt gttgaaaatg cggttgtccg cgatgggttt
      961 ttcggtcacc ccttgtacga cggggcagga tgcggtcgga gaggcgaaga aaacgatgtt
     1021 taacttggcg atcactgact tgcgccttcg cggggaagat gggttggacg tgaccgaaga
     1081 actcctcagg agtcatccgg gattgcccgt catcatcctg acggcccatg ggagtattcc
     1141 caatgctgta gaggcgatgc aacggggggc gttcgggtac ctgacgaagc cgtttgatga
     1201 taaggagctg aaagccacga tcgagaaagc gctcgcgcaa cagcggatga gccaggagat
     1261 tcagcggctc aagtcgttgg tcaaagaact gtacgggctg gaaaatgttg tggcgcggag
     1321 tccggcgatg cagcggctct tccagcaaat cgcgcaggtg gccgattcgg atgcgaccat
     1381 tttgctgttt ggagaaaccg gaaccggcaa ggaggtgatg gcgcgcgtca tccatacgaa
     1441 cagccggcgc gggaagggtc ctttcgtggc gctcaactgc gcggcgatac ccgaaacgct
     1501 gttcgagagc gagttgttcg gacatgtgaa gggcgcgttc acgagcgctc ttggcgcgaa
     1561 gcgcggattg tttcagatgg ccaacggcgg cacgctgttc ctcgacgaga tcggggagat
     1621 gccactgtcc atgcaggtca aattgttgcg tgccgtacag gagcgggaga ttcgagaggt
     1681 ggggtcagaa acttcggtca aggtagacgt tcgcatcatt gcggcgacca ataaggacct
     1741 cggcgaggcg gtgaagaacg ggacgttccg caacgatcta tactatcgaa tctcagtggt
     1801 gcctctgttc attccaccgc tgcgggatcg ccgggacgat atcccattgc tggctcaaca
     1861 tttcctcaag ctcagcgtga agcgggcgaa caaggaagtg aagggattca cgcccgcggc
     1921 ccttcatcgt ttgatgatca atccctggcc ggggaatgta cgcgagcttg agaacgcggt
     1981 cgagaaggcc gtggtgatgt cacggcaaga tatgctcacg ccggatttgc tgccggcggt
     2041 cagtgtgtcg gccgattcgc ctctcaagcc gctgacagag gccaaagaag aattcgagcg
     2101 gacatatttg aagaatgttc ttcaattgac gggggggaat atctcgcgtg ccgcacagtt
     2161 tgccggccgg taccgagccg atttctataa gatgctgagg aaatatggtc tccatccgtc
     2221 aaccacgaaa ggaaggccgg attcggacat ggaagagttc gaaagcgaag cgaatttgac
     2281 tgaggcggaa cgatagatct tcgatcccat cgatcttgga agattcatag gtggattgaa
     2341 agggccgcgt ctccggtgac gcggcccttg tgtttcagca gatgcatcga actcttgatc
     2401 atcggttatc gctgtatacc tttttcattg gactccccga tcttcgagcg cttaggatat
     2461 cgacggtgac tgccattcta ttgagaaagg agaggggcta tgatgcggga ggggaaatcc
     2521 atgattgtgc cggccggagc gttgatcatg gccataaccg tcgcactatc accgtcgttg
     2581 gtcctcggcg agggaggagc gcgtcgtgat gtggtccgtc aggcaggcga actggcggcc
     2641 gctgaaaatg ccgcaccatc gtcactttcg aagaatgcca ccattctcaa tcgagatgga
     2701 caagtggttc gcatcggaaa caatgggtgg atgtgtttgc cggatgatcc tagtacggca
     2761 ggaacagact cgatatgtat gaacgaatcg tggcgtaatt tcatggaagc attgaagaac
     2821 aagaagaaac ccacgtatac ccaagtaggg attgcctata tgctccaggg cgaccgccct
     2881 gtgagtaata ccgatcc
//
LOCUS       DASTWE010000057         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1197096, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000057 DASTWE010000000
VERSION     DASTWE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197096"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1086)
                     /locus_tag="VFT30_00645"
     CDS             complement(16..1086)
                     /locus_tag="VFT30_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091158.1"
                     /translation="MSHCCSDSSYAGLILFLIVLSFCLEQADAKDFNPDNPLNQPAPV
                     YWCSSKTPDQQISTKKSSGCEPLYDQQTAESFQENARQQGFELPDRDPIKIVELQNAA
                     SKFSDRYRTFVSCCLTDNHAPAEIVDLIDEANHILKAIQQKGIFNSAGFGIGSGNGGL
                     GGGAAQGPKLGTFARQFTLSEIVGTVARARADLFRLKSRLDTLNEAQQGLSEVDYETS
                     GRTKAQIQEDEEAIKKEFKTKKPPSSAPTGMEIQDTTLRSRIGGDIEDTKLNSHFGAD
                     IGASVSPYSNVGESLRPRRGDDVHDSQLPHRPGSDIQDTSMSSSTGFEVDSAQNREGA
                     STLPRRGVGASIGDSDLNSRKR"
BASE COUNT          264 a          299 c          329 g          336 t
ORIGIN      
        1 gccgtaagga ttggactatc ttttccggct gttcaggtcg gaatctccaa tagaagcacc
       61 gactccgcgt cgtggaagag tggaagctcc ttcacgattt tgcgcgctgt cgacttcgaa
      121 cccagtactg ctggacatgg aagtgtcctg tatatccgac cccggccgat gcgggagttg
      181 gctgtcgtga acatcatccc ctctccgcgg acgaagtgac tctcctacat tgctataggg
      241 agataccgag gctccgatgt ccgcaccaaa gtgtgaattt aatttagtgt cctcgatgtc
      301 tcctccaatt cgtgagcgca aggtcgtgtc ttgaatttcc atcccggtcg gtgctgatga
      361 tggtggcttt ttggttttga actctttctt gatcgcctct tcatcctctt ggatctgcgc
      421 tttggtccgg ccgctggttt cataatcgac ttcgctgaga ccttgctgtg cctcgtttaa
      481 tgtatccagg cggctcttga gtcgaaacag gtctgcacgt gcacgtgcga cggttccaac
      541 aatttcgctg agcgtgaatt gtcgagcaaa cgtgccgagc ttcgggcctt gggccgcgcc
      601 gccgcccaaa ccaccgtttc cagagccaat accgaatccg gcggaattga aaataccttt
      661 ttgttgtatg gctttgagta tgtggttggc ttcgtcgatc aaatcgacga tttctgcggg
      721 tgcgtggttg tcggtcaagc agcatgagac gaacgtccga tagcgatctg aaaatttgga
      781 ggcggcattc tgcaattcca cgatctttat aggatcgcgg tcgggaagtt caaacccctg
      841 ttggcgtgca ttttcctgga aagattctgc cgtctgttgg tcgtagagag gctcacaacc
      901 cgaactcttt ttcgttgaaa tctgctgatc gggtgtcttg ctcgaacacc aatagaccgg
      961 tgcgggttgg ttgaggggat tatcagggtt gaagtccttc gcatcggcct gttctaagca
     1021 gaaggataga acgatgagga acaggatcag tcctgcgtat gaggaatccg aacagcagtg
     1081 agacatgaaa gcattctcta attgaaatcc cttgaagtca actaaactct ccgagtttga
     1141 tgctaggaat gtatccgttt gttcctagag accaagtcgc gtagcctatt gtaattgaaa
     1201 aacaaattga tccgaagcgg ctgctcgt
//
LOCUS       DASTWE010000058         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_324588, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000058 DASTWE010000000
VERSION     DASTWE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324588"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..>1702
                     /locus_tag="VFT30_00650"
     CDS             386..>1702
                     /locus_tag="VFT30_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5091159.1"
                     /translation="MTQPTPQERHEAVSLSDAAIRALQNNLDISLSRHTKESRLTDII
                     FEQSKFDPTLTVNGQYFRTVDPLERPVLGATGSELNQITKFDQRNHALSVDASTPLVT
                     GGNFDVNYSPARYSANLAEGFLFNPAWTGGLSFSVTQPLLKNAGIAVNTTFIKVAQNN
                     AVVEQHIFRDRVLTVIASVEQNYWELVFAKENVKVAQAALKAAEELLATNRLKAKGGV
                     MSIVDVLQAEAAVASRVEQVLVAEKAIRDEEDQLRTILNPGEAELRQNVRLTPVDAPI
                     TYLETLSLEEAIETAIEQRPEIVQAKRHIESSELNKQFARNQLLPTLSFQGTMGLTGL
                     GGDYSESFMRNFSGSFYNYGAGLVFSYPLGNRAAISTYTRRQLEAKNAEITLASVRHQ
                     IILGIREAVRRVQTDFKRIETTRSARILAEKQLQTEQERLKVGLSAT"
BASE COUNT          417 a          447 c          460 g          378 t
ORIGIN      
        1 gcctgaagcg gaggggacga agtcgccata tggtcttcat ggcacacatc tcgttgtggg
       61 tccgcagtct gctgatcaca catgacggta tattgaaacc tgatcagcca tccggatcga
      121 gaaagtcaca gcctgcgcac gactactccg tgtgagggac aggttgggta gtctcgtctt
      181 attaatagga tgaggtgctg atctcctatg gaggtaatgg tctatccata ctcgttcggt
      241 cgatgacatg tcggctctcg gcgggaactc tgtttatgga aggagtgtta tggattgcag
      301 gcgttcggct cattacagag tcatagctct tatgatcgtt ggtttgtgtg gcattgtgtc
      361 tccatctcgg agttggagct tggatgtgac acaaccgact ccgcaggaac gtcatgaagc
      421 ggtgtcgctg tcggatgcgg ccattcgcgc tttgcagaac aatctcgaca tcagtctgag
      481 ccgccatacc aaagagagcc ggttgaccga catcatcttt gaacagtcga agttcgatcc
      541 cacgttgact gtgaacgggc aatatttccg gacagtcgac cctcttgaac gaccggtgtt
      601 aggggcaact ggtagtgaac ttaaccaaat cacgaagttc gaccagcgta atcatgccct
      661 gtcagtcgac gcttcgaccc cacttgttac gggcggcaat ttcgatgtga actatagccc
      721 ggcaagatac agtgcgaatc tcgccgaggg attcttattc aaccccgcct ggaccggcgg
      781 tttgtcattc tcagtgaccc agccgctgct caagaatgcc gggattgcag tgaacaccac
      841 cttcatcaag gtcgcccaaa acaatgcggt cgtcgagcag catatttttc gggaccgggt
      901 tttgacggtc attgcctcgg tcgaacaaaa ctattgggaa ctggtgtttg cgaaagaaaa
      961 tgtgaaagtc gcccaagccg cgctgaaagc agccgaggag ctattagcca cgaatcgcct
     1021 caaggccaaa ggtggtgtga tgtccatcgt cgatgtgctc caagcggagg cagctgtggc
     1081 gtcccgagtg gagcaagtct tggtggctga aaaggccatt cgtgatgaag aagatcaact
     1141 gcggactatc ctgaatccgg gcgaagccga gttgcggcaa aatgtgcgtc ttactcccgt
     1201 cgatgcgccc attacatatc tcgagaccct tagccttgaa gaagccatcg agacagcgat
     1261 cgagcagcgc ccggaaatcg tccaggcaaa aaggcatatc gagtccagcg aactcaataa
     1321 acaatttgcc cgcaaccaac tactccccac gctatcattt caaggcacca tgggccttac
     1381 cggactcgga ggggactata gtgaatcctt catgagaaac tttagtggga gcttctacaa
     1441 ctatggcgca ggcctggtgt tcagctaccc gcttggcaat cgcgctgcca tcagtacata
     1501 cacaaggcgc caattggaag ccaagaatgc cgaaatcacc ctcgcgagcg tccgtcacca
     1561 aattattctc ggcattcgcg aggccgtgcg ccgcgtgcag accgacttca aacgcatcga
     1621 aaccacacgt tcggcacgca tcttagctga gaaacaattg caaactgagc aagaacggtt
     1681 aaaggtgggg ctcagcgcca ct
//
LOCUS       DASTWE010000059         1367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_550916, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000059 DASTWE010000000
VERSION     DASTWE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550916"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1367)
                     /locus_tag="VFT30_00655"
     CDS             complement(<1..>1367)
                     /locus_tag="VFT30_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5091160.1"
                     /translation="FPVVIRLTGQLNLKAVEHGFNEVVRRHEVLRTTFCSIDGKPYQI
                     IAPSLHIPLDILSIDSASGDEQESSLRRLATEEGNRPFDIARGPLMRTTMYRLAEDEH
                     VLVLTMHHIIADGWSFPVLMKELVTFYQAYVEGRPASSPPLAIQYADFAQWQHNWLQG
                     SVHETQLAYWKRQLDGSLPVLELPLDRPRPTVQSYRGKRYVVDFSPELSVALQALSCK
                     QGVTLFMTLLACFQILLARYSGQTDIIIGTPVANRTRSELESLIGFFVNMLVLRVDLS
                     GNPRFTDLLKRVREIVLGAQAHQDLPFEKLVDALQPTRALSHSPLFQVIFVLHSLPPQ
                     TLELPGLRIHMPEMDPETAKFDLSLDIFPDPDKMTGWFEYNADLFDEQTIARMAQHFV
                     LLLETIVAAPDSYLEAMPSLIATDREQVVERWNATERTYPTWLTVPELISAQAAASPT
                     AVAVR"
BASE COUNT          331 a          357 c          391 g          288 t
ORIGIN      
        1 cccgcacggc caccgcagtc ggcgatgccg ccgcctgcgc tgaaatcaat tccggcactg
       61 tcagccacgt cggatacgtc cgttccgtcg cgttccacct ttccaccacc tgttctcgat
      121 cagttgcgat aagcgaaggc atggcctcga gataactgtc gggagcggcg acgatcgttt
      181 ccagcagaag cacaaaatgt tgtgccatcc gagcaatcgt ctgctcatca aaaagatcgg
      241 cgttgtattc gaaccacccg gtcatcttgt ccggatccgg gaagatgtcc aacgagagat
      301 cgaacttggc ggtctccggg tccatttccg gcatgtgaat gcgcaggccg ggcaattcca
      361 aggtttgcgg cggaagacta tggagaacga atatgacctg aaacaacggc gagtggctga
      421 gtgcgcgggt cggttgcaac gcatcgacaa gtttttcgaa cggcaagtcc tgatgggcct
      481 gtgctcccag aacgatctcg cgcacgcgct tcaataaatc ggtgaaccga ggattgccgc
      541 tgagatcaac tcgaagcacg agcatgttga caaagaaacc gatcaagctt tccaattcgg
      601 aacgtgttcg attggcaacg ggtgtaccga tgatgatgtc ggtctggccg ctgtaacgag
      661 ccagcaatat ctggaagcag gcgagcaagg tcatgaacaa tgtgacccct tgcttacagc
      721 tgagagcttg cagcgcaacc gacaattcgg gagaaaaatc tacgacatac cgtttccctc
      781 gatagctttg tacggtcggc cgaggacgat ccagcgggag ttcaagtacg gggagagatc
      841 cgtctaattg gcgcttccaa tatgccagct gagtttcatg cacggatccc tgtagccagt
      901 tatgctgcca ctgcgcgaaa tcggcatact gaatcgccaa ggggggcgat gaggcaggac
      961 gtccttccac gtaagcctgg tagaacgtca ccaattcctt catgaggacg ggaaatgacc
     1021 aaccatcggc aatgatatgg tgcatggtca gaacgagcac atgttcgtcc tcggccagtc
     1081 gatacatggt ggtgcgcatc aagggaccac gcgcgatgtc gaatgggcga tttccctctt
     1141 ccgttgccag tcgacggagc gaggattcct gctcatcccc ggaagcggaa tcaatcgaaa
     1201 ggatatcgag tggaatgtgc agtgaaggag cgatgatctg atagggtttt ccatcgatgc
     1261 tgcagaacgt ggtgcgcaat acttcatgac ggcggacgac ctcgttgaat ccatgttcga
     1321 cagctttgag attcaattgt ccggtcagtc gaatgacgac aggaaag
//
LOCUS       DASTWE010000060         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_938620, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000060 DASTWE010000000
VERSION     DASTWE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938620"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3357)
                     /locus_tag="VFT30_00660"
     CDS             complement(<1..>3357)
                     /locus_tag="VFT30_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU5091161.1"
                     /translation="GLPTLTVQYVDYADWQRTRLDQGLRDVHRAYWTRQLSGALPPAE
                     LPVDRPRLRVRTFEGGVRSRSLSPELSADLDLFCQHWNVTTFMVLYAVFATWLHRYTH
                     ESDVIIGSIVSGRRRRELEDVMGYCVNTVALRSELSDGMTGQELLKQVRRVVVDAYDH
                     QELPFEEVIEALSLQRARQLSPLFNVMMVCEDNPLSAFTVKDLEVAHLPWEPTASEFD
                     LVLMVVNKMDGLDLAFLHDSTIFDDSTIDRMLGQLEILLEEFLKKPEARLDQLSLLTA
                     EERRNLQLTWNKPCHSATGIHSLIEAQVERTPHAPAVICGDQSISYQELNRRANRVAR
                     ALKKLGVGTDLPVGLCVERSVDALVGLLGILKAGGGYVPLDPSFPSHRLQLILEDAHV
                     SIVVTQAYLHNHLQSYGGQICDVETLSMFSGSAAEEENLAIPVSPDQLAYIVYTSGST
                     GRPKGVAVTHRSLVTSLHARLQHYPEPISRFLLTFSLAFDGSVTGIFWTLLQGGELVI
                     PSETAHRDPTELAALIEHHRISHVVWVPSLYHAILGEALSAQLESLRVIITAGESLPL
                     ELVRRHYQLLPHATLYNEYGPTEATVWCSVYQTTREEAGARVPIGKPIDHMQLYVLDA
                     NLQPMPIGVPGELYVAGECLARGYVNQPQLTQERFLANPYVAGTRLYRTGDLARYRAD
                     GNVEFLGRVDQQVKLRGYRIELGEIEYVLNNFPGVHQAAVLLRQDKPDQHRLVGYVAG
                     ESSVRERLDQIRGHLAARLPHYMVPSVILWLETMPLSATGKVNRHALPAPEHTADLAA
                     ARIAPRNQVEESLAELWKSVLQVPEAGIHDQFFEQGGHSLVATQLVSRIRELFEVDVS
                     LPAFFERPTIAALAEEVTRLRQREQKASAMPPIIPVPRDQPLPLSYSQQRMWLMYRLA
                     PESTAYNMPFASRQMGRLNKTALRSTIDAICSRHEAFRTTFMMRGEGPVQMIHPFRSP
                     HWVEVDLQELPPEQRRQKAARLVEQEANQPFDLEKGPLARFLLIEIEHEDHVLVLTMH
                     HIIGDQWSFGVIGHEFAFFYNAFCRGEVPSAKPISLQYADYAVWQRRCLTDDRLNTQS
                     DYWRKKLAGLSKLSLPTDYPRPPVQTFNGSH"
BASE COUNT          764 a          949 c          943 g          701 t
ORIGIN      
        1 atgtgaacca ttaaatgttt gcaccggggg tcgcgggtaa tcggtcggca acgacagctt
       61 ggaaagtccg gccagcttct tccgccaata atccgattgc gtgttgagcc ggtcgtcggt
      121 cagacaacgc cgttgccaca ccgcgtagtc ggcatactga agggagatcg gctttgccga
      181 aggaacttct cctcggcaaa aggcgttgta gaagaatgca aactcatgac cgataacccc
      241 gaacgaccac tggtctccga tgatatgatg catcgtgagg accaacacgt ggtcctcgtg
      301 ctcgatctca atcaagagaa atcttgccag agggcccttc tcaagatcga acggctgatt
      361 cgcttcctgt tccacaagcc gagccgcctt ctgtcgtcgc tgttcggggg gcagttcctg
      421 gagatccact tcgacccaat gaggagaacg aaaggggtgg atcatttgga ccggtccctc
      481 tcccctcatc atgaacgtcg ttctaaaggc ctcgtgtcgg ctacagatgg cgtcgatcgt
      541 acttcggagg gctgttttgt tgagccgtcc catctgccgc gaagcaaacg gcatgttata
      601 cgctgtgctc tcaggagcca gccgatacat cagccacatg cgttgctgag aataagaaag
      661 gggaagcggc tgatctctgg gcacaggaat gatcggcggc atcgctgaag ccttttgttc
      721 cctctggcgc aaccgcgtca cttcttcggc caatgccgcg atagtcggtc gttcaaaaaa
      781 cgccggtagc gagacatcga cctcaaaaag ttcgcgtatg cgggagacga gctgggtagc
      841 caccaaggag tggccgccct gctcgaagaa ctgatcgtgg atgcctgctt cgggtacttg
      901 aagaacggat ttccaaagct cagccaagga ttcctcgacc tgattcctcg gagcaatcct
      961 ggcggccgcc agatcggctg tgtgttctgg agcgggaaga gcatgtcgat tcacttttcc
     1021 ggtggcactg agcggcatcg tttccagcca cagaatgacg gatggcacca tgtagtgcgg
     1081 taatcgagca gcgagatgac cgcgaatctg gtcgagccgc tctctcaccg acgactcgcc
     1141 ggcgacatag ccgaccaatc ggtgctggtc cggcttatcc tgtcgaagca gcaccgccgc
     1201 ttgatgcacg cccggaaagt tattcaacac atactcgatt tctcctagtt caatccgata
     1261 gccacgcagt ttgacctgct ggtccacccg tccgagaaat tctacattgc cgtcggcgcg
     1321 ataccgagcc aggtcaccgg tccgatagag cctggtgccg gccacgtagg gattcgcaag
     1381 aaatcgctcc tgggtcagct gcggctggtt gacgtaaccg cgtgccagac attctcccgc
     1441 gacatagagt tctcccggta cgccgatagg catcggctgc agatttgcat ccagcacata
     1501 gagctgcata tggtcgatgg gtttgccgat tgggactcgg gctccggctt cctcacgagt
     1561 cgtttgatac acgctacacc agacagtggc ttccgtcggt ccatattcgt tgtacagtgt
     1621 ggcatgaggc aggagctgat aatgtcgccg cacgagttca agtggcagac tttctcctgc
     1681 agtaatgatg acacggagcg attccaactg agcactcaac gcttcgccga gtatggcatg
     1741 gtacaaagaa gggacccata cgacatgcga aatacgatga tgctcgataa gcgcggccag
     1801 ctccgttgga tcgcgatggg cagtctcgga tggaatgacc agttctccac cctgcagcaa
     1861 cgtccaaaat atgcctgtca cggatccatc aaaggccaga gagaatgtca gcaagaaccg
     1921 ggaaatcggt tctggatagt gctgaagtct ggcatgaaga gaggtcacta gactgcggtg
     1981 tgtcacggcc acgcccttgg gccgtcccgt cgagcccgac gtgtagacga tataagcgag
     2041 ctgatccggg gaaacaggaa ttgctaggtt ctcctcctcc gctgcgctcc cgctgaacat
     2101 cgacaaggtc tcgacgtcac agatttggcc gccatagctc tgtaaatggt tatggaggta
     2161 tgcttgtgtg accacgatgg aaacatgggc gtcctccagt atgagctgga gacggtgact
     2221 ggggaaggac ggatccaagg gaacataacc gccccctgct ttaagaatgc ccaagagacc
     2281 cacgagggca tccaccgagc gctcgacaca gagacccacg ggaagatccg ttccgactcc
     2341 taatttcttg agcgcgcgag cgactcgatt cgctcgtcgg ttcagctctt ggtagctgat
     2401 ggactggtct ccacagatca cagctggtgc gtggggagtc cgttcaacct gagcctcaat
     2461 gagcgaatgg atgccggtgg ccgagtgaca tggcttgttc catgtcagtt gaaggttccg
     2521 tctctcctcg gcagtcagca atgaaagttg atcaagccgg gcctcaggtt tcttgagaaa
     2581 ctcttcaaga aggatctcca gctgtcccag cattcggtcg atggtcgagt cgtcgaaaat
     2641 cgtagaatca tggagaaacg ccaagtccag gccgtccatc ttgttgacca ccatcaacac
     2701 gaggtcgaac tccgacgcag tcggctccca ggggagatga gcgacctcga gatccttgac
     2761 ggtaaacgcg gacaaggggt tgtcttcaca caccatcatc acgttaaaca gcggggacag
     2821 ctgacgcgct cgctgtaacg acagcgcctc gatgacctct tcaaacggta gttcctgatg
     2881 gtcataggca tccaccacta cccggcgtac ctgcttcaac aattcttgtc ctgtcatccc
     2941 atccgacaac tcggaccgca acgccaccgt attgacgcaa tagcccatca cgtcttcgag
     3001 ttccctccgg cgcctgccgg acacgatcga tcctatgatg acatccgatt cgtgtgtgta
     3061 gcggtgcagc caagttgcaa agacggcgta cagcaccatg aacgtcgtca cgttccagtg
     3121 ctgacagaag aggtcgagat ccgctgaaag ttccggagaa agcgatcgtg accgcacgcc
     3181 tccctcgaag gtgcgcaccc gcagccgcgg acgatcgacc ggtagctcag cggggggaag
     3241 agcgccactt aactggcggg tccaataggc tcgatggaca tcacgaaggc cttgatcaag
     3301 cctagttctc tgccaatcgg catagtccac atactgaaca gtcaacgtgg ggagtcc
//
LOCUS       DASTWE010000061         5814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1455677, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000061 DASTWE010000000
VERSION     DASTWE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455677"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1289)
                     /gene="fabF"
                     /locus_tag="VFT30_00665"
     CDS             complement(33..1289)
                     /gene="fabF"
                     /locus_tag="VFT30_00665"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5091162.1"
                     /translation="MASRVVITGLGVISPIGIGVSEFWKAALAGRSGVTAIPSLGWFP
                     MSGYRSRVAGQVHNFSPEQYLSTTQASRVDRYAQFALVSTKEALADANLIMAKEIPHR
                     VGVIVGAGMGGMVMGEREITQLFKTQRPHRVHPNFIPTITLNSASGIVAMAHGAKGPN
                     LTISTACSSSAHALGQALQCIRSGQADVIIAVGADASITPLVFAGFCSLRALSSEFND
                     APERASRPFDRRRDGFVMGEGAAALVVESLAHAKKRKARVYAELAGYAATSEAYHMVI
                     PQEDGQEISTTMKIGLDSAGIGPDQVDYIKAHATSTTIGDAVETKAIRSLFKNRAEKI
                     AINATKSLIGHTLGAAGAIGAVATTLSIHTGHIHPTANYEEPDPACRLDGIRRAVQDR
                     KVRYALLNAFGFGSNNATVVFKKFVA"
     gene            complement(1367..2458)
                     /gene="lptG"
                     /locus_tag="VFT30_00670"
     CDS             complement(1367..2458)
                     /gene="lptG"
                     /locus_tag="VFT30_00670"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEU5091163.1"
                     /translation="MTILFRYILREYAKIFGMCFSGLVTIYLVIDFFEKVRRFLRYES
                     GIVPILTYFALKIPSISFQVAPFAILVATLLTLGLFARSNEITAMRSCGISLLWMTSP
                     FLLFAGGVSAILFLFSSTVIPLASEKAEEVRVIQIEQRPAPVTVKAPQPWARISADGL
                     MEVNEIDLAGKILRGVRIFYFRPPFNLDRITEAAEARYTPQGWILLNGNHRRFQSDDT
                     VELALFTEQAISIPLIPDDFSSSLVGNSETMTFREIRNYLARFRHEGFSFARLLTDYY
                     GRVAFPLVTVVMVLVGIALSLRRSGVRGGNMAVGIGQAFIVGFCYWTTHSIAIALGRG
                     GVLAPMLAGWMANALFLSFGLYLLLKVRH"
     gene            complement(2463..3554)
                     /locus_tag="VFT30_00675"
     CDS             complement(2463..3554)
                     /locus_tag="VFT30_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU5091164.1"
                     /translation="MILRTLLDRYIFTELLSPFGLSLGALCFVMLTRELLRLVELLVS
                     KGVGFWAVLKVIAMLLPSGLVLTLPIAGLIASVTAFGRLSYDKELVAMRATGLSLFRL
                     SQPVLLFSVCVFTLTLVLSQWGQPWSSLNLKKVALNLLKDQLVLALERGTFNEPIPRM
                     MIYVPESEPGQPAEGIFISDERLPEEPRVIVAEQYHVFMDAAHAQVALQLVNGVIHSR
                     PRQVDHYRQITFASYDLKIDLNLSSYSATEERPSYEQIMARLAESGGKDAGSLRRLME
                     HYKDLAFPTASLVFCLLGVPVGIVSKRSGRIGGFAVGVGIIIAFYILNVGCDFLVTAL
                     ILDPFWGAWLPNIIFVVITAALFFKMSRQ"
     gene            3846..4064
                     /locus_tag="VFT30_00680"
     CDS             3846..4064
                     /locus_tag="VFT30_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5091165.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDSGEDVFVHYSALQGDGFKTLKE
                     GENVEFDIVQGAKGPQAANVLKSAIAAS"
     gene            4238..>5814
                     /locus_tag="VFT30_00685"
     CDS             4238..>5814
                     /locus_tag="VFT30_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5091166.1"
                     /translation="MPSDRSRVLQQAQLLASRGQYEAAIAEWKKLAAETPADGTIHNT
                     IGDLQLRRNALGEAASAFLQAASAFRAEGATLKAIAAFKKVLKCDPSRYEVYRHLGDL
                     NIERGLISSAVQDYLTLGKYYLKEHRGKDALEIYKKIVLQDPSNLDAQQRVAELCVQE
                     NQQDEATKVYLQLGRERSAQGRYDEAKEAYLSVLRIDPANNEAVQFVESMKKGGTGSM
                     KAVKPGSIAPVQKPSEPVDLLAEAVRRIDERQYAGAEAILNQMLTREPGNPQVCQLLA
                     RLHLQRGDVQVALGEYRFLAGAALRAHDLALAESLIQEFLSADPNSVPLLELNGELHE
                     ENGDGEGAALQYAKAIELLLEHPEPGMESLHEELFEKVRSLSRDEDLVNRLTARMGGG
                     STVDQSESVQNVSGLEAPSNLINPISTDSGSLRPDSGGAHTVRPDDREFTLVGAEPDN
                     GNPFAKDGGPRQRKVESDAVSNGQESHTMPVTASPETSRQLQTESVKISSVGKSSASQ
                     TSDGQKAEIQASLVSHST"
BASE COUNT         1466 a         1532 c         1625 g         1191 t
ORIGIN      
        1 gaaggggtcc tcgatacgtt caaccgtata tcttatgcca caaatttttt aaagacgacc
       61 gtggcattgt tgctgccgaa tccgaatgcg ttcaagaggg cgtaccgaac cttgcggtct
      121 tggacggcac gtcggatccc atccaaacgg caagctgggt ccggttcctc gtagttcgct
      181 gtggggtgga tgtgtccggt gtggattgag agggtcgtcg ccacagcgcc gatcgcccca
      241 gccgccccca acgtatgccc gatgagtgac ttcgtggcat tgatggcgat tttctcagca
      301 cgattcttaa aaaggctcct gatcgctttg gtctccacgg catcgccgat cgtcgtggag
      361 gtcgcatggg ccttgatgta gtcgacctga tccggcccga tgccggcaga gtctagaccg
      421 atcttcatgg tcgtggagat ttcctgacca tcttcctgag gaatgaccat gtgataggct
      481 tcgctggtgg cggcatatcc ggcaagttca gcatagactc tcgcttttcg cttcttggcg
      541 tgcgccaatg attccacgac taatgcggca gccccctcgc ccatgacgaa tccatcacgg
      601 cgtcgatcaa agggacgtga ggctcgctcc ggagcatcgt taaactcgct ggagagggca
      661 cgcaaggaac agaacccggc aaagaccaac ggagtaatgc tggcatcggc tccgacggcg
      721 ataatgacat cggcctgacc gctgcgaatg cattgaagcg cttgaccaag agcatgggca
      781 ctggaggaac aggccgtcga gatcgtgaga ttcggtccct tggcgccatg tgccattgcg
      841 acgatgcctg aagcggaatt caacgtgatc gtaggaatga aattggggtg cacgcgatgc
      901 ggtcgctggg ttttaaaaag ctgtgtgatt tcacgctctc ccatcaccat cccgcccatg
      961 cctgctccga caataacacc gacgcgatgg ggaatctcct tggccatgat cagattggca
     1021 tcggcgagcg cttccttcgt ggagaccaac gcgaattgcg cgtaacgatc cacgcgactg
     1081 gcttgcgtag tcgacaaata ctgttccggt gagaaattgt ggacttgccc ggcaacgcgt
     1141 gaacgatagc cggacatggg aaaccatcct agagaaggga tcgcggtaac tcctgagcga
     1201 cctgcgaggg ctgccttcca gaattcgctg acgccgatcc caatggggga tatgaccccg
     1261 agaccggtaa ttaccacgcg cgaggccatg tttctccctc cttatgagcg cacacggcgc
     1321 ctttcttacc ggaagaccga tgtacagtca actaggaaac ctttcttcaa tgtctaactt
     1381 tcaataacag atagaggccg aagctgagga acagtgcatt ggccatccag ccggcaagca
     1441 taggagccaa gactccccct cgtccgagcg cgatagcgat ggaatgtgtc gtccagtagc
     1501 agaatccgac gataaatgct tgtccgatac ctacggccat gtttccacct cggactccgc
     1561 ttcgccgcaa acttaacgca atgccgacga gcaccatcac gaccgtcact aatgggaatg
     1621 caacacggcc gtagtagtcc gtcagcagac gggcgaacga gaaaccttcg tgccgaaagc
     1681 gcgcgaggta atttcggatt tcccgaaacg tcatggtttc tgagttacct accaatgagg
     1741 aggagaaatc atcgggaatc aatggaatac tgatcgcctg ttccgtgaac agagcgagct
     1801 ctacggtgtc gtccgattgg aaccggcgat gatttccgtt cagcaggatc cagccttgag
     1861 gagtatagcg ggcttcagcc gcctcggtga tccgatcgag attaaacggc gggcgaaaat
     1921 aaaagattcg tacgcctcga aggatcttac ctgcaagatc gatctcattg acttccatca
     1981 ggccgtcggc gctgatacgg gcccagggct ggggggcttt gacggtgacc ggagccggtc
     2041 tttgctcgat ctggattaca cggacttctt cggctttctc cgaggcgagc ggaataacgg
     2101 tcgagctgaa gaggaagaga atcgcagaca cgccgccggc aaacaggaga aacggcgagg
     2161 tcatccacaa caagctgatc ccacaactgc gcatggcggt gatttcgttg ctgcgcgcga
     2221 acagaccgag cgtcagcaag gtcgccacca agatcgcgaa cggagcgact tggaacgaaa
     2281 tagaaggaat cttcaacgcg aagtaggtga ggatgggcac gattcccgat tcatagcgca
     2341 agaaccggcg gactttctca aaaaagtcga tcaccagata gatggttacc aaaccggaaa
     2401 agcacatgcc gaaaattttg gcgtattcac gaaggatgta ccggaatagg atcgtcatga
     2461 tgctattgcc tgctcatctt gaagaatagg gcggcagtaa taacgacaaa tatgatattg
     2521 ggcagccagg ctccccaaaa cggatcaagg atcaaggccg tcaccaaaaa atcgcacccg
     2581 acatttaaga tataaaacgc gatgatgatc cccacgccga cggcaaaccc accgatcctt
     2641 ccggaccgct tcgagacaat tccgaccggc acgccgagca gacagaatac gagcgaagcg
     2701 gttggaaagg ccaagtcctt atagtgttcc atcagacgac gaagagagcc ggcatctttg
     2761 ccaccggact cggccagccg tgccatgatc tgttcatagg acggacgctc ttcggtggcc
     2821 gaataactgc tgaggttcag atcgatcttg aggtcataac tggcgaatgt aatctgccga
     2881 taatggtcga cctgacgcgg tcggctatga atgactccat ttacaagctg gagcgcgacc
     2941 tgcgcatggg ctgcatccat aaagacgtga tattgttccg ccacgatcac gcgaggctct
     3001 tcgggcaagc gttcatccga gatgaagatg ccctcggctg gctggccagg ctcgctttcc
     3061 ggaacataaa tcatcatacg gggaatcggc tcattgaagg tgccccgttc caacgccaac
     3121 acaagctgat ccttgagcag attgagagcc accttcttga gattcaatga gctccatggc
     3181 tgcccccatt gagacaatac caatgtcaac gtaaagacgc acaccgagaa gaggagtacc
     3241 ggctgcgaga ggcgaaacag gctgagcccg gttgcacgca tggcgaccag ttccttgtcg
     3301 taggacaacc ggccgaatgc tgtaaccgac gcgatcaagc ctgcgatcgg aagcgtgagc
     3361 acgaggccgg agggaagcag catggcaata acttttaaca ctgcccagaa accgaccccc
     3421 ttggacacca gcagttcgac aagacgcaag agctccctgg tcaacatgac aaagcagagc
     3481 gcgccgaggc tgagaccgaa cggagagaga agttcagtga aaatataacg atcgagcagt
     3541 gttcgtagga tcacagaatg tttggatcgg agaaattttg aacactatag gggagtcctt
     3601 ctcacctgta aacacaggag aaaatcctcg cctgaaagat tcccttgaca gagcatgaag
     3661 cctatgttac atgcagcgcc actttccctc aaatgatggg ttccgtgagc gatgtatcat
     3721 tccttcaggt gcccgtccct tctaccatcc gaattcttgc ccaatctatt ttcaattttg
     3781 atacgggacg attgagtggg gccgctacgt aaccagtcgt agttgtttac aaaaggaggg
     3841 cttctgtgaa gacaaaagga acagtcaagt ggttcaacga tcgaaaaggg tttggcttca
     3901 tccggctgga tagcggagaa gatgtcttcg tccattattc cgcgttacaa ggagacggtt
     3961 tcaagactct caaggagggt gaaaacgtgg aatttgacat cgtacaggga gccaagggcc
     4021 ctcaagcggc caatgtgctc aagtccgcga tcgccgcatc ctaagtcgta ctatcttcag
     4081 tttgctcggt agaagagccg atcctcgaca agagtggtcg gcgcttctac tcgagcgttt
     4141 cattgtctga ttctgccatt tcctagacaa aaaccattga tactgttagc gtttagcttg
     4201 ttctgcgttt agctatgctg ttcgtggagg gatcgttgtg ccttcggatc gaagtcgagt
     4261 tcttcaacag gctcagctcc tggcgtcccg cggacagtac gaagcggcca tagccgaatg
     4321 gaagaagttg gccgcagaaa cccctgccga cggcactatt cataatacga tcggtgattt
     4381 gcaattgagg cgaaacgctc taggggaagc cgcctctgca ttcttgcagg cggcctcggc
     4441 cttccgagcc gaaggcgcca ccctgaaggc aattgcggct tttaaaaaag tccttaaatg
     4501 cgatccttct cgatacgaag tctatcgcca ccttggagat ctgaacattg aacgcggact
     4561 catcagcagt gcggttcaag actacctcac ccttgggaag tactacctaa aagaacatcg
     4621 gggaaaagac gcgctcgaga tctacaaaaa gatcgtcctg caggatccgt cgaatctcga
     4681 cgcgcaacag cgagtcgccg aactctgtgt ccaggaaaac cagcaggacg aagccactaa
     4741 agtgtatctc cagctaggcc gtgagcgatc ggcgcaagga agatacgatg aggcgaaaga
     4801 ggcgtatctc tccgtactca ggatcgatcc agcgaacaac gaagcggttc aattcgtgga
     4861 gagcatgaag aaaggcggaa cggggtcgat gaaagcggtc aaaccaggat ccatcgcgcc
     4921 ggtgcagaag ccgtctgaac cggtggactt actggctgag gccgttcgcc gcattgacga
     4981 aaggcagtat gccggcgcag aagcgatcct gaaccaaatg ctcacccggg aaccgggaaa
     5041 tccccaagtc tgtcaactcc tggctcggtt gcatttgcaa cgcggagatg tgcaagtggc
     5101 gttgggggaa tatcgcttct tggccggggc agcgttgcgg gcacatgacc tggctcttgc
     5161 tgaatcgctg attcaggagt tcttgtccgc cgacccgaac tcggtcccct tactggaact
     5221 gaacggggaa ctgcacgagg agaacggtga tggcgaggga gccgcactgc aatatgcgaa
     5281 ggccatcgaa ctcttgttgg agcatcccga gcccggcatg gaaagtctgc atgaagagct
     5341 cttcgaaaag gtgcgatcgc tgtcccgtga tgaagacttg gtgaatcgac tgacggcaag
     5401 aatgggtgga ggatccacgg tagatcagag cgagtctgtt cagaacgtgt ctgggttgga
     5461 ggcgccatcg aacctcatca atccaatatc gacggatagt ggatcactga ggcctgattc
     5521 gggcggtgcc catacagtcc gtccagacga tcgagaattt acgctggtag gtgcagagcc
     5581 agacaacggc aatccttttg cgaaggacgg tggaccgcgg caaaggaaag tcgagtcgga
     5641 cgcggtttcg aatggacaag aatctcacac catgccggtc accgcatcac cggagacctc
     5701 acggcaactt caaaccgagt cggtgaagat ttcatcggtt gggaaatcat ccgcatcaca
     5761 aacaagtgat gggcaaaagg cagaaatcca agcatcactt gtttcacatt caac
//
LOCUS       DASTWE010000062         1859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1294109, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000062 DASTWE010000000
VERSION     DASTWE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294109"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFT30_00690"
     CDS             complement(<1..992)
                     /locus_tag="VFT30_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5091167.1"
                     /translation="MAKKYVYYFGDGKAEGTSNMKELLGGKGAGLAEMTNLGISVPPG
                     FTISTEACVEYYRIGKQYPHGMWDATLQALKRVERSMGMGFGNPERPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVEGLAAKTRNDRFAQDSYRRFVSMFGSIVMGVPREHFEAI
                     LKHKKTEVGVAHETHLDARHLRELVASFKALIKEETKKDFPDEPLEQLRLAINAVFSS
                     WFGARAITYRRLYSIPDSWGTAVNVVAMVFGNMGETSGTGVAFTRSPSSGDRAFFGEC
                     LMNAQGEDVVAGIRTPLPVSALSKNVPEAYKELEHTYKKLEKHYRDMLDLEFTI"
     gene            complement(1021..1512)
                     /locus_tag="VFT30_00695"
     CDS             complement(1021..1512)
                     /locus_tag="VFT30_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091168.1"
                     /translation="MDQPMTDSRRRADKIRRFSLAVSLALMVLCNAILLGGLALSGIN
                     LDDLVKTPDFFNAKQDVCLRLTWQSVPGAKEPVRLCSEWLNLSDPSGKTHYLQPDTKL
                     KKGLDGQYYVDQGVQADYRLLMLMLFVTAIIIGGVRAKWFLVNRYRLRLESTDGRRAS
                     PAH"
     gene            complement(1575..>1859)
                     /locus_tag="VFT30_00700"
     CDS             complement(1575..>1859)
                     /locus_tag="VFT30_00700"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEU5091169.1"
                     /translation="PVDEARFQDAAESALHKAVGNERGRIEASVKAGEYSQALDALVS
                     LKPAVDAFFEAVMVNAEDKAIRGNRLSLLKMVDELFMEFADFSQIVVQGR"
BASE COUNT          395 a          564 c          475 g          425 t
ORIGIN      
        1 tggatcgtga attcgaggtc aagcatgtcc cggtagtgtt tctcgagctt cttgtaggtg
       61 tgttcgagct ccttataggc ctcaggaacg tttttcgata acgcgctcac cggcaggggc
      121 gttctgatcc ccgcgacgac atcttcgccc tgagcattca tcaaacattc accgaagaat
      181 gctcgatcac cggaactcgg gcttcgcgta aacgcgacac cggtcccgct tgtttctccc
      241 atgttcccga acaccatcgc caccacgttc actgcggtcc cccaggaatc gggaatgctg
      301 tacaaccggc ggtaggtgat ggcgcgggcg ccgaaccagg aggaaaatac cgcattgatt
      361 gccagccgca gctgttccag cggttcatcc ggaaagtctt ttttcgtttc ttctttaatg
      421 agggctttga aactcgccac taattcgcga aggtgcctgg catccaaatg ggtttcatgc
      481 gcaacgccga cttcggtttt cttgtgtttg agaatcgcct cgaaatgctc acgcggtaca
      541 cccattacga tactgccgaa catcgacaca aatctccgat agctgtcctg cgcaaaccga
      601 tcgttgcgcg tttttgccgc taacccctcc acggttttcg tggtcagacc gacgttcagc
      661 acggtgtcca tcatgcccgg catggaggca cgcgcgccgg atcggaccga gaccagcagc
      721 ggccgttcag gattcccgaa ccccatcccc atcgaacgtt ccacgcgttt gagcgcctgc
      781 aaggtggcat cccacattcc gtgagggtac tgcttgccga tcctgtaata ctcgacgcag
      841 gcttcggtag aaattgtaaa tccgggcggg actgagatac ccaaattggt catctcggcc
      901 aaccctgcgc cttttcctcc gagcagttct ttcatgttcg atgtgccctc ggctttgccg
      961 tcgccgaaat agtagacgta tttctttgcc acgtgacatc tccttccagg aagagtgcta
     1021 tcaatgagcg ggcgacgcgc gacgaccgtc ggttgattcc agacggagcc gatagcggtt
     1081 caccaagaac cacttcgccc ttacgccacc gatgatgatc gcggtgacaa aaagcatcag
     1141 catcaataac cgatagtcag cctgaacacc ttgatcgacg tagtactggc cgtcaagccc
     1201 cttcttgagc ttcgtgtcgg gctggagata atgggtcttt ccactcgggt cggagagatt
     1261 aagccattcc gaacaaagcc gaaccggttc tttcgctccg ggcactgact gccacgtgag
     1321 tcgaagacac acgtcctgct tggcattgaa gaaatcgggc gtcttgacca agtcgtccag
     1381 attaatgccg ctgagcgcga gcccgccgag aagaatagca ttgcagagca ccatcaaggc
     1441 cagcgagact gccagagaaa atcgccggat cttgtccgcc cgacgtcggc tgtccgtcat
     1501 cggctgatcc atggcagctc cagtcttgcc ttaaatctcg ccggcaggat gcccgtctct
     1561 tagtccgatt tgacctaccg tccttgtacc acaatctggg aaaagtccgc gaattccatg
     1621 aacaactcat cgaccatttt aagcagcgac aacctattgc cgcggatggc cttatcttca
     1681 gcattcacca tcacagcttc gaaaaaggcg tcgacggccg gcttaagact taccagcgcg
     1741 tccaatgctt ggctgtactc gccggcttta accgaagcct ctatccggcc gcgttcgttt
     1801 cctacggctt tgtggagggc cgactcggcg gcatcctgaa accgcgcctc gtccaccgg
//
LOCUS       DASTWE010000063         1493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_809392, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000063 DASTWE010000000
VERSION     DASTWE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809392"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..>1493)
                     /gene="pheT"
                     /locus_tag="VFT30_00705"
     CDS             complement(4..>1493)
                     /gene="pheT"
                     /locus_tag="VFT30_00705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5091170.1"
                     /translation="KQQGRLKPYSFFAKKPKSPHHLIVKPGMEQVRPYVAACTARGYR
                     VTEKGLAQLIQTQEKLADIFGRKRKTVSIGIYQLRNITFPVTYELVKPDDVRFTPLGM
                     ETTMTLSEILMVHPKGLEYGPILAVKSLLPILRDAKDQPLSFPPIINSREIGEVRVGD
                     DELFIEVTGTDLPMVMLSLNIFAANLADRGAIIEPMEVQYSTSTTLGKRVKTPQDFGM
                     PKTIRTKAIEQALGQELGETVVKQALEAYGYGVSAEKGSVKARLPPYRHDLMHTMDIV
                     EDVAMSRGYGEFTPIMPAQFTVGGLSTIEQLSDRSRELMVGLGFQEIISNILGSPDHY
                     SRLMRLDGTEWGRMVEVDNVMSLNFSCLRQWMLPSLLRIEAASSRAFYPHRLFEAGDV
                     AIPDSTHELGSRTETVLGALIAHATTHFSEIHSSLDVLFYYLGREYSLEPIQHPSFLE
                     GRAGRILVSGKSVGVIGEVHPEVLERWQIAMPVVAFDVNLSQLAE"
BASE COUNT          346 a          424 c          386 g          337 t
ORIGIN      
        1 cttctattcg gccagctgcg agaggttcac gtcgaaggcg acgaccggca tagcgatctg
       61 ccaacgttcc aggacttctg gatggacttc gccgatgacg ccgacggatt tgcccgagac
      121 gagaatgcgg ccggcacggc cttctaaaaa agacggatgc tgtatcggct caagactgta
      181 ctccctgcca aggtagtaaa acaggacatc gaggctggaa tggatttctg aaaaatgggt
      241 ggtggcatga gcaatcaagg cgcccaagac ggtctccgtt cgagacccca actcgtgagt
      301 actgtcggga atcgccacat cgccggcttc gaagaggcga tggggataga acgcccgact
      361 ggaggcggct tcgatgcgca gcaatgaggg cagcatccat tgtcggagac aagagaagtt
      421 caacgacatg acgttgtcaa cctcaaccat acgtccccat tccgttccgt ccagacgcat
      481 aagacgggaa taatgatccg gtgagccgag aatgttcgaa atgatttcct gaaacccgag
      541 ccccaccatc aattctcggg atcgatcgga cagttgttca atggtggata atcctcccac
      601 cgtaaattgc gccggcatga tgggggtaaa ctcgccataa ccccgactca tggcgacgtc
      661 ttcaacgatg tccatcgtat gcatcagatc gtgccgatac ggcggaagcc ttgctttaac
      721 tgatcctttt tctgccgata cgccataccc atacgcttca agagcttgct tcacgactgt
      781 ctcaccgagt tcctggccca acgcctgttc aatcgctttg gtccgaatcg tcttgggcat
      841 cccgaaatct tgtggcgttt tgacccgttt gcccaacgtc gtgctcgtcg aatactgtac
      901 ttccatcggc tcgatgattg cgccacggtc ggccaaattc gccgcgaaga tattcagcga
      961 aagcatcacc atgggtaagt ctgtccccgt cacttcaatg aagagttcat catcacccac
     1021 ccgtacttct ccgatttccc gactattgat aatcggtgga aatgacaacg gttggtcctt
     1081 cgcgtctcgg aggatgggga gcagactttt gacggccaga ataggcccgt attccagccc
     1141 cttgggatga accatgagaa tctccgacag ggtcatcgtc gtttccatac ccaatggggt
     1201 aaaccgcacg tcgtccggct tcaccaactc ataggttacc ggaaaggtaa tattccgcag
     1261 ctgatagatt ccaatggaaa cggtctttcg cttgcgccca aagatgtcgg ccaacttttc
     1321 ctgcgtttga atcagctgag ccaaaccctt ctcggtcacc cgataccccc tggccgtaca
     1381 ggcagccacg tacggacgca cctgctccat accgggcttc acgatcagat gatgcggcga
     1441 cttcggtttt ttggcgaaga aggaataggg cttcagccgg ccctgttgct tga
//
LOCUS       DASTWE010000064         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1068063, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000064 DASTWE010000000
VERSION     DASTWE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068063"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1219)
                     /locus_tag="VFT30_00710"
     CDS             complement(<1..>1219)
                     /locus_tag="VFT30_00710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5091171.1"
                     /translation="YASADFRLGHALAADYYERVTKRDKAGNRQQSHVDRIADDESRL
                     TVLELGVGNGNLAACFLSHLKALDKQGTIYPRVRYVMVDWQQTVLDGALAHPELAVHR
                     EQVETHQGSVEHLAEIAEASVDRIICNELWNDLPTKLLAKHSGEVEEEYLRPNLSESL
                     HANIQDWSTFVLAFQEIDLATLKTFPSFLDELVWEKEYRKVEWKDAPYRKTIVEFLQS
                     IDQEVLVPVNVGAFATLKEAKRLLAPDAIGFSAFDAGTGDMKVLNDPEKPCYGQFGGQ
                     YNFMVNFALIQAVAKHVGVKHTTLESQREFVGRSLNTNVITVMDLLAVHPSAGPKLQA
                     WEQDRLVLQTIKALNETFTSSYRRRIDFPLSTDMPPEERETSNAILRSLKDDGIPDTV
                     AYLTEEELTRAQQD"
BASE COUNT          248 a          333 c          339 g          299 t
ORIGIN      
        1 aatcttgctg tgcacgagtg agctcttctt cagtgaggta cgctacggtg tccggaatgc
       61 cgtcgtcttt cagggagcgc aggatggcgt tcgacgtttc tcgttcttca ggcggcatgt
      121 cggtgctgag gggaaaatca atccggcgac gataggagct tgtaaatgtc tcgtttaacg
      181 ctttgatggt ctgcaacaca agtctgtctt gttcccaagc ttgcagtttt ggcccggcag
      241 acggatgcac cgcgagcaga tccataaccg taatgacgtt tgtattcaac gaccgcccga
      301 cgaattctcg ttgcgattcg agtgtcgtgt gcttgacccc cacatgcttg gccacggcct
      361 ggatcaacgc aaagttgacc atgaagttgt attggccccc gaactggccg tagcacggtt
      421 tctccggatc gttgaggact ttcatgtcgc cggtgcctgc atcgaaggca ctgaagccga
      481 tcgcatcggg agccaggagc cgcttcgctt cttttagggt cgcgaaggcg ccgacgttga
      541 ccggcaccaa gacttcttgg tcgatggatt ggaggaattc gacgattgtt ttccgatagg
      601 gcgcgtcctt ccactcgact ttgcgatatt ctttttccca gaccagttca tcgaggaagg
      661 aagggaaggt cttgagcgtt gccagatcta tttcctgaaa tgcgaggacg aacgtcgacc
      721 aatcctgaat gttggcatgg agcgactcgc taaggttggg acggagatac tcctcctcga
      781 cctcgccgct atgtttcgct aacaatttcg tcggcagatc attccacagc tcgttacaga
      841 tgatccgatc gacactggcc tcggcgattt cggccaaatg ctcgacagat ccttgatggg
      901 tttcgacttg ttctcgatgc acggctagct caggatgcgc cagagcccca tccagcaccg
      961 tttgttgcca atcgaccatg acataccgca cacgcggata gatcgtgcct tgtttatcga
     1021 gggccttgag atggctgaga aagcaagcgg cgagattccc gtttccgacc ccaagctcca
     1081 ggaccgtcaa gcgcgattcg tcatctgcta tccggtcaac atgcgactgt tggcgatttc
     1141 cagccttgtc gcgcttcgtg acacgctcgt aataatcggc cgccagcgca tggccgagac
     1201 ggaagtccgc cgatgcgta
//
LOCUS       DASTWE010000065         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1520458, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000065 DASTWE010000000
VERSION     DASTWE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520458"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="VFT30_00715"
     CDS             complement(<1..1951)
                     /locus_tag="VFT30_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091172.1"
                     /translation="MAAISRATHAISFQLLNKDGQSVLGIFDPQKGQTSTLELINSSR
                     RNWKLKTLTGVPSATNHHFELKFRSDTLSLTGTPAITVDADAAKWKISSPVPTEGGVS
                     LYLLSESPFELKSGTKATVKLNNLNAAGTGGGRGTRVELKWGGNLEYSNSTGPTPDPW
                     VAGHRVQHLTLVNQRGEQNIPLHVGFSPTNQVLNNGTVNELKLQITNAMTQGSINVIP
                     QGDPEFQATEFKVSFESSANKWTLAPLDAVKQFEIKASLEGRELKVRKEEQGQTISWI
                     IWADRLPRLALNPGKSIDVTISNVKTSYLSGPTNLYLHYKNIPGYWDGDFVSIIEKGP
                     FVYRDKGVGVGTNDPQTKLHVKGTTRLEGDASIENGNLSVGTTTTRKKLSVNGDLRVE
                     DEISVGGGHAVKVDAPGVPAGRFYVTTDGDVGIGTADPKSKLHVNGDVRVAKDKEIYF
                     VDNGQIRSHDDAHRILFRREGYNVLELREYGHIVFSPGSEQGAETAKMIIRNTGRVGI
                     GTDDPSTHLHIKGAGDQEVMIESSDDNGAKWSLQSSAGAQDGRFEIINRSSGQNHFTI
                     LKDGRIGIGTVGPTKAKLEVSGWVGGSIGTFAYMNGSVFRSGGTSSPNDYSIWASNRI
                     AALEFNAFSDERVKNIQGRSDSGMDL"
     gene            complement(1974..>2542)
                     /locus_tag="VFT30_00720"
     CDS             complement(1974..>2542)
                     /locus_tag="VFT30_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091173.1"
                     /translation="PQILSLLIDPRGSVHATSGIVPVKAINIPPDQYGAALQSIEITF
                     LNAPIITDAGKIHLPLPEEAGYQWSWLHDNRGKWSEVSSRGVVRKETFAVEFESKATD
                     VWNELLELGWIELDKDDPVRAFVTEKDKRGSSTLNRFKELTERIEDVLDRAHIGPVNL
                     AAKFTGPQEIREGWLKLSTAGQAKTPKP"
BASE COUNT          493 a          705 c          692 g          652 t
ORIGIN      
        1 aaaggtccat cccgctgtca gagcggccct ggatgttctt aacgcgttcg tcagagaagg
       61 cgttgaattc taacgcagcg atgcgattcg aagcccagat agagtaatcg ttcggcgatg
      121 acgtcccacc tgacctaaac actgaaccat tcatgtatgc gaatgtgccg atcgaaccac
      181 caacccagcc ggaaacctcc agcttcgctt tggttgggcc aaccgtcccg atgccgatcc
      241 tgccatcttt taggatcgta aaatgattct gcccggagga gcggttgata atctcgaagc
      301 gcccatcctg agccccggcc gacgactgta acgaccattt ggcgccatta tcatcagagc
      361 tctcgatcat tacctcctgg tcgccggcgc cttttatgtg taggtgcgtt gatggatcgt
      421 ccgttccgat gccgaccctg cctgtgttac ggattatcat cttggccgtc tctgcccctt
      481 gctcggagcc gggggaaaag acgatgtgac catattcccg cagttccaat acgttatagc
      541 cttcgcggcg gaagagtatg cggtgggcat catcgtggct tcggatctgg ccgttgtcga
      601 caaagtagat ttctttgtct tttgcgactc gcacgtcgcc gtttacgtga agcttagact
      661 tcgggtcggc tgttccgatg ccgacgtcac cgtcggtagt cacatagaac cggccggcag
      721 gcacgcctgg cgcgtcaacc tttaccgcgt gtccaccccc aactgagatt tcatcctcaa
      781 cgcgcaggtc accgttaaca gacagttttt tgcgagtggt tgtcgttccg acactgaggt
      841 tgccgttctc gatcgaagcg tctccctcga ggcgtgttgt cccttttaca tgaagcttag
      901 tttgcgggtc gttcgtgcct acgccgactc ctttatccct gtagacaaac ggtcccttct
      961 cgatgattga tacgaagtcc ccgtcccagt agccggggat gttcttgtag tgcaggtaaa
     1021 ggttagttgg acccgagaga taagaggtct tgacgttcga gatcgtcacg tcgatcgatt
     1081 tcccgggatt cagcgcgagc cgcggtagcc tatcggccca aatgatccat gagatcgtct
     1141 gaccctgctc ttccttcctg accttgagtt cccttccttc gagtgacgct ttgatctcga
     1201 attgcttgac cgcgtcaagc ggcgcgagtg tccacttgtt agctgaggat tcgaacgaaa
     1261 ctttgaattc tgtggcttga aattcagggt cgccctgagg aatcacgttt atggatccct
     1321 gtgtcatggc attggtgatc tgtagtttga gctcatttac agtcccgtta ttcaacacct
     1381 gattagtggg ggagaagccc acgtgaagcg gaatattctg ctcacctcgc tggttgacga
     1441 gggtcaggtg ctgcacgcga tgtccggcaa cccatgggtc aggtgtcggt ccggtggaat
     1501 tggagtattc cagattgcca ccccacttga gttcgactct ggtgcccctc ccgccaccag
     1561 tgcccgccgc gttgaggttg ttcagcttaa cagtggcctt cgttccggat ttgagttcga
     1621 agggactttc actcaacaga tagagagaca cgcctccctc agtcggcact ggtgagctga
     1681 tcttccactt cgctgcgtcc gcgtcgacag tgatcgcagg tgtgccggtg agactcaaag
     1741 tgtcggacct gaacttcaat tcgaaatggt ggttcgtcgc cgacggtact ccggtaagtg
     1801 tcttcagttt ccaattgcgc cgcgagctat tgatgagctc aagagtagac gtctgtccct
     1861 tttgagggtc gaagatgccg agaactgatt ggccatcctt gttaagcagt tggaagctaa
     1921 tcgcgtgggt ggctctgctg atcgcggcca taaaatctct ccttggttaa ctgttagggc
     1981 ttgggggttt tcgcctggcc ggccgtgctc aatttgagcc agccttcgcg aatctcctgc
     2041 gggccggtga acttcgctgc caggttcaca ggtccgatgt gtgcgcggtc gaggacgtct
     2101 tcgatccgtt cggttagttc tttgaaacgg ttcagcgttg aactccctct tttatctttc
     2161 tccgtgacga acgcccgaac cggatcgtct ttgtcgagct caatccagcc aagttcgagc
     2221 agctcgttcc agacgtcggt tgctttgctt tcgaactcaa cagcaaacgt ctctttgcgg
     2281 acaacgccgc gcgacgaaac ttcggaccac ttgccacgat tgtcatgcaa ccaggaccac
     2341 tggtatcccg cttcctcggg cagcggcagg tgaatcttgc ccgcgtccgt aatgatcggc
     2401 gcattgagga aagtgatctc gattgactgc aacgccgcgc cgtattgatc cggaggaatg
     2461 ttgatcgcct tgacaggcac aataccgctc gtcgcatgga ccgaaccgcg cgggtcgata
     2521 agcagactca gaatttgcgg at
//
LOCUS       DASTWE010000066         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_227859, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000066 DASTWE010000000
VERSION     DASTWE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227859"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFT30_00725"
     CDS             complement(<1..637)
                     /locus_tag="VFT30_00725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5091174.1"
                     /translation="MRYNLDTSGKFKIVMNTYRVFGFWETLKEVVLYLLSCQTPNDDF
                     DKKYGVMTSGFTESFEAGITDETARSLAVGYVPTREVVIRHILKNTTKGLDLKEYSFI
                     DLGCGKGRTLIVASQLPFKEVIGVELSPLHCEVATANVEGYLSNGRHPVLCRNVRVDC
                     VNAADFEFPDTNLLIYMYRPFIGPIFKSVADRVRHFQAVCGRRVLIAYSCPH"
     gene            complement(650..>3243)
                     /locus_tag="VFT30_00730"
     CDS             complement(650..>3243)
                     /locus_tag="VFT30_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU5091175.1"
                     /translation="IEVQADKTPDAVAIVCGKRSLTYHELNRRANQLAHYLRQHGVGP
                     DVLVGVCMERSVEMVVSLLGILKAGGAYLPIDPDYPQDRIEFMLKDTQPVLLLTQRHL
                     FAQFQGYSGHALYLDTDWPLIAHQSTMNPQVSLSPLNLAYTIYTSGSTGRPKGVGNTH
                     LGLLNRLQWMQDSFHLRTGDRVLQKTPFSFDVSVWEFFWPLMTGAGLVVAGPGEHRNP
                     ARLIELIKEHNVTTIHFVPPMLHVFLETAGVDTCRCLQRVICSGEALSVELQRRFFMQ
                     LTWAELHNLYGPTEAAIDVTAWICKRNDDATSVPIGRPIANTQIFLLDRQDQTVPIGV
                     PGELHIGGVGVARGYHRRPALTAEKFVPDPFSKIPGSRMYKTGDVARNREDGVIEYIG
                     RLDHQVKVRGFRVELGEIEAWLSKHSAVREVVVLAREDQPDDKRLVAYIVPVSMPSRL
                     TPTANDLRQFLSKQLPEYMIPAKFLFLAELPRTANGKIDRKLLPAPDFSAQLIDQYVA
                     PRTAIENKLAGMWAEVLNVERIGIYDNFFDLGGHSLLAVQLMSRIRKSIGGSLSLLAI
                     FQAPTVASLAELLSNSRHDSSSSLVAFRDTGSCPPLYCFDPDGAHVLAYQPLGYSLDD
                     EQPVFGISLSRILSLDWRTLSIASIAEDHVSLIRQHQPEGPYNLLGWSNGGIIALAVA
                     HALERQDQAVSFLGILDTQPRVGMTENLNVLDELFAYVSRDHQREFLSLPEEERQALQ
                     THLVSLTEENRVEYAIQWAQKRGFLSHEESETSIDVLKIGYALDKEANRALQAYRHMP
                     VRAPIHAWWASATLSKHGKAPVDWTLYTRGTVEIETILGDHTDAVQSIQVHQRISEIL
                     AMNRSVT"
BASE COUNT          780 a          899 c          811 g          753 t
ORIGIN      
        1 catgtggaca cgagtatgcg atcaagactc gacgtccgca aactgcttga aagtggcgca
       61 cgcgatcagc gacgcttttg aaaattggtc ctatgaaagg acggtacata taaatcagca
      121 gattggtgtc cggaaactcg aaatccgccg cattgacgca gtccaccctg acattcctgc
      181 aaagcacggg atgtcgcccg ttcgataaat atccctcgac gttggccgtc gccacctcgc
      241 agtgcaacgg agaaagctcg acgccgatta cttccttaaa cggcagctga gacgctacga
      301 tcaaggtacg gcctttccca catcctaaat ctatgaagga atactctttg agatcgagac
      361 ccttcgtcgt gttcttcaga atatgccgaa tgacgacctc tctcgtgggt acatacccta
      421 ccgccaagct tctagccgtt tcatcggtaa tcccagcttc aaatgactcc gtgaacccgg
      481 aggtcatcac cccgtatttc ttgtcgaaat cgtcatttgg tgtctggcaa ctcaagagat
      541 acaggacgac ttccttgagg gtctcccaga agccgaatac ccgataggta ttcatcacga
      601 tcttgaactt tccgctggtg tcaaggttgt accgcatatg actccttctt cacgtcacag
      661 acctgttcat tgccaggatt tcgctgatgc gttgatgaac ttggatactc tgcaccgcat
      721 cagtatggtc accaagaata gtttcgattt ctacggttcc tcttgtatac aaggtccaat
      781 caaccggagc ttttccgtgc ttacttaagg tcgcactagc ccaccaggca tgaatcggag
      841 ctcgcacggg catgtgacga tacgcttgca aggcccggtt cgcttccttg tccaaagcat
      901 atccgatttt tagtacatca atggacgtct ccgattcttc atgcgacaag aatccacgtt
      961 tttgtgccca ttgaatcgcg tactccacac ggttttcttc cgtcaggctg accaagtgtg
     1021 tctggagcgc ttgccgttcc tcttcaggca aactcaaaaa ctcgcgttga tgatcacggc
     1081 tcacgtatgc gaacagttcg tccaagacgt taagattctc cgtcatcccg acacgcggtt
     1141 gcgtgtccag gatgcccagg aacgagacag cttgatcttg gcgttccaat gcgtgcgcaa
     1201 cggccaaggc gataattccg ccattggacc atccaagaag attataagga ccttccggct
     1261 ggtgctgacg tatgagactt acgtgatcct ccgcaatgga tgcaattgac agcgttctcc
     1321 agtctagaga caagatccga cttaatgaga ttccgaaaac aggctgctca tcatcaaggg
     1381 aatacccaag cggctgatac gcaaggacat gtgctccatc cggatcaaag cagtacagtg
     1441 gggggcacga tccggtgtct cggaacgcta ccagcgaaga ggaggaatca tgtctgctgt
     1501 tcgagagaag ctcggcaaga cttgccacag tgggggcctg aaagatcgcg agcaaagaca
     1561 atgagcctcc aatggatttg cgaatacgag acatcagctg aacggccagc aacgaatggc
     1621 cgccaagatc gaagaaatta tcgtaaatgc ctatccgctc gacattgagc acctcagccc
     1681 acataccggc cagcttgttt tctatcgctg tgcgaggtgc tacatattga tctatgagct
     1741 gagcactgaa atccggtgcc ggcagcaact tgcggtcgat tttcccattg gcagttcgtg
     1801 gcaactcggc tagaaataga aacttagcgg gaatcatgta ttcgggtaat tgtttggata
     1861 gaaactggcg taggtcattt gccgtaggag tcagccgaga gggcatacta accggcacaa
     1921 tatatgccac aagccgtttg tcgtcaggct gatcctctct ggccaacacc acaacctccc
     1981 tcactgcgga atgcttgctc agccacgctt cgatctcccc cagttcaacc cgaaaccctc
     2041 tgaccttcac ttgatggtct aagcgcccta tgtattcaat aactccatct tcccggttgc
     2101 gcgccacatc accggttttg tacatgcggc tgcccggaat tttgctgaag ggatcgggca
     2161 caaatttctc cgccgtcagc gccggccgcc ggtgatagcc gcgtgcaaca ccgacgccac
     2221 caatatgcag ttcgccaggt actcctatgg gcaccgtttg atcctgtcgg tccagtaaaa
     2281 agatttgcgt atttgcgatc ggacggccga tcggcaccga tgttgcgtcg tcattccgct
     2341 tacaaatcca ggcggttaca tcaattgcgg cctccgtggg tccatagaga ttgtggagct
     2401 cagcccacgt cagttgcata aaaaatcgac gctgcagttc gacagacagc gcttcgccgc
     2461 tacagatgac acgctgcaaa catcgacaag tgtccacgcc ggctgtctcc aggaatacgt
     2521 gaagcatggg aggtacgaag tggatcgtgg tgacgttgtg ctccttaatc aactctatca
     2581 atcgcgcggg atttctgtgc tctcccgggc ccgcaacgac aagaccagct cctgtcatca
     2641 acggccagaa gaattcccac accgagacat caaagctgaa cggtgtcttc tgcagtaccc
     2701 ggtcccctgt ccggagatga aaggagtcct gcatccactg aagccgattg agcagcccga
     2761 gatgggtatt cccaacacct ttggggcgac ctgtcgagcc tgacgtatag atggtataag
     2821 cgagattcag gggcgacagg ctcacctgag ggttcatggt ggattgatgg gctatcaatg
     2881 gccagtcggt atcgagataa agcgcatggc ctgagtaacc ctggaattgc gcgaagagat
     2941 gacgctgggt caacagtaat accggctgtg tatccttgag catgaattcg atccggtcct
     3001 gtggatagtc tggatcgatt ggcaaataag caccgccggc ctttaaaatc ccaagcaggc
     3061 tcacgaccat ttcaacagac cgctccatgc acactcccac gagaacgtcc ggtccaaccc
     3121 catgttgccg gaggtaatgc gccagctgat tggctcgtcg attcagctcg tgatatgtca
     3181 gagaccgttt cccgcacacg atggcgactg cgtccggtgt cttatccgcc tgtacttcga
     3241 tca
//
LOCUS       DASTWE010000067         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_33975, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000067 DASTWE010000000
VERSION     DASTWE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33975"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFT30_00735"
     CDS             <1..895
                     /locus_tag="VFT30_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEU5091176.1"
                     /translation="TISDQPLVELMPYIDWSPFFHTWELRGRYPTIFDDPTVGPKAKE
                     LYDDARRLLDEIVQKRQLKARGVYGFFAAASVGGDDIELYADASRKIRVTTIHHLRQQ
                     SEKPTGQPNLSLADYVAPKESGRQDYIGAFAVTAGIGLDELCKRFDNDHDDYNSIMAK
                     ALADRLAEAFAEFLHKRVREEWGYGKKERLTNDDLIRERYRGIRPAPGYPACPDHTEK
                     KLLFELLHVEKNTGITLTESFAMLPAAAVSGFYFAHPDAKYFAVGKIGKDQVEDYARR
                     KGMDLRTVERWLSPNLNYEPE"
     gene            988..1593
                     /locus_tag="VFT30_00740"
     CDS             988..1593
                     /locus_tag="VFT30_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091177.1"
                     /translation="MQTRILIVDDEQDILSALKKWLTWMGHEVVTSEDGEQALKVVAE
                     EQPDLMLLDIELPGLSGLDILKQLAEKRSSIPPQSVPEVIVITAFGTIDRAVEAIRLG
                     ACDFLTKPFEPDHLSIVIEKTMAQMALTRQIGLLQAEVKGRYEHVIGQSPRMVELLET
                     ARRAAGSSATVLLLGETGTGKEVMARAASVEPTCHEIFCRR"
BASE COUNT          387 a          410 c          449 g          351 t
ORIGIN      
        1 gacgatctcc gatcaaccgt tggtcgaact gatgccttat attgattggt cacccttctt
       61 ccacacgtgg gaattaagag gccgctatcc gacaattttt gatgatccaa ccgttggacc
      121 aaaggccaag gaactgtacg acgatgcgcg acgtctcctg gatgagatcg tccagaagcg
      181 acagctcaaa gctaggggag tctacggatt ttttgctgct gcgtcggtcg gtggtgacga
      241 catcgaattg tacgcggatg catcgcggaa gatccgggtg acgacgattc atcatcttcg
      301 tcagcaatcg gagaagccga cgggccagcc caatctttcg ctggccgatt atgttgcgcc
      361 gaaggaatca ggccggcagg attacatcgg agcattcgcc gtcacagccg gtatcgggtt
      421 ggatgaactg tgcaagcggt ttgacaatga ccatgacgac tataattcga ttatggccaa
      481 ggctctcgcc gacaggctag ccgaagcctt cgccgaattc ctccataaac gggtcaggga
      541 agaatgggga tacggcaaga aagaacgatt gacgaatgac gatctgatcc gagagcgata
      601 ccgtgggatc cgtcctgcgc cgggatatcc ggcttgtcca gaccacacag agaagaagtt
      661 gctgttcgaa ctcttgcatg tagaaaaaaa taccggcatc acattgacag aaagctttgc
      721 catgttgccg gctgcggccg tgagtggatt ctatttcgcc catcctgacg ccaagtactt
      781 tgccgtcgga aaaatcggca aagaccaagt tgaagactac gcgcgccgca aggggatgga
      841 tcttcggact gtcgaacgct ggctctcacc gaatctgaat tacgaacctg aataatattt
      901 gcgtacgctc ggtagccggc ggactttctc ttcagtccta tcttccgcta caatagccca
      961 tcatgattct ctccctaagg gtgttcgatg caaacacgta ttctcatcgt cgatgatgaa
     1021 caagatattc tcagtgcgct caagaagtgg ttgacctgga tgggtcatga ggtggtcacg
     1081 tccgaggatg gtgaacaggc gctgaaagtg gtggccgaag agcaacccga tctgatgctg
     1141 ctcgacattg aattaccagg gctcagcgga ctcgatatcc tcaaacagct cgccgaaaaa
     1201 cgttccagca ttccgccgca gtctgtcccg gaagtgatcg tcatcacggc gttcgggacg
     1261 atcgatcgtg ctgtagaagc gatacggctc ggggcatgcg atttcctgac aaagccattt
     1321 gagcccgatc acctctccat tgtgattgag aagaccatgg cacaaatggc tctcacccgt
     1381 cagatcggcc tgctacaagc agaagtgaag ggacggtatg agcatgtgat cgggcaaagt
     1441 ccacgcatgg tcgagttact tgagacggca cggcgggcgg ccggctcttc cgccacggta
     1501 ctgttattgg gggaaacagg gacgggcaaa gaagtcatgg cgcgcgctgc atcggtggag
     1561 cccacgtgcc acgaaatctt ttgtcgtcgt taactgc
//
LOCUS       DASTWE010000068         1656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_809523, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000068 DASTWE010000000
VERSION     DASTWE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809523"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFT30_00745"
     CDS             <1..649
                     /locus_tag="VFT30_00745"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5091178.1"
                     /translation="IEKGAQWFKSIGPESGPGPKLFCVSGHVEKPGLYELPMGIPLRT
                     LLYDHCGGIRGGKKLKAVMPGGSSFPLLTPDQIDAPMDFDSMRKINAFLGSAGVIVMD
                     EDTCMVKALEIIDRFYHHESCGQCSPCREGTGWLHKLLIRLEEGNGKLEDIELMDKIS
                     TNMTGNTVCVLADAAAMPTQSFLSKFKDEFVTHVTLGRCPFKKEGGVPRHHRHAA"
     gene            769..>1656
                     /locus_tag="VFT30_00750"
     CDS             769..>1656
                     /locus_tag="VFT30_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5091179.1"
                     /translation="MPTIEIDGKKVTVPDGWNVIQAAEKLDIEVPHYCYHPGLSISGN
                     CRMCLVEIEKMPKLQIACNTKVTEGMVVKTASPKAKAAQTAVLEFILVNHPIDCPICD
                     QAGECKLQDYYMDYGKHHSQVPLEAKVEKKKVVDIGPQVVLDQERCILCARCTRFLDE
                     VTKTGELGLFARGDHTYIDLFPGKRLENPYSANVVDVCPVGALTHKDFRFKARVWYLE
                     KVESVCTKCANGCNIDIYHRRGQMFRFRPRSNPDVNQYWMCDEGRLWYKELQKESRIL
                     RPVAREDDAFMPISWDQAVG"
BASE COUNT          398 a          399 c          473 g          386 t
ORIGIN      
        1 tattgaaaag ggcgcccaat ggttcaagtc gatcggtccg gaaagtggtc cgggaccgaa
       61 acttttctgt gtcagtgggc acgttgaaaa acctggtctt tacgaattgc cgatgggaat
      121 tccattacgg acgcttctgt acgatcactg tggcggcatt cgtggcggca agaaactgaa
      181 agctgtgatg cccggcggtt cgtctttccc actgttgact ccggaccaaa ttgatgcgcc
      241 catggatttt gattccatgc gcaaaatcaa cgcttttctc ggttccgcag gcgttattgt
      301 catggacgaa gatacttgca tggtaaaggc gttggagatt atcgaccgtt tttatcatca
      361 tgaatcctgc gggcagtgtt cgccgtgtcg tgaaggcacc ggttggctcc acaagttgtt
      421 aatccgcctc gaagaaggta atggcaagtt ggaagacatc gagctgatgg ataaaatttc
      481 gaccaacatg acgggaaaca ctgtgtgtgt gttggccgat gctgcggcga tgccgacgca
      541 gagcttcttg tcaaagttca aagacgagtt tgtgacgcat gtgacacttg ggcgttgccc
      601 ttttaagaag gaaggcggtg taccgcgtca ccaccggcat gcggcgtaaa gcagtcacga
      661 gttatgggtg atgggctatg agtgagaaaa agggacgagc gtcaaaggtc atactactct
      721 ccgctctgtg ctcgttactc gtcactcggt accgtacttg aggaaacaat gccaacaatc
      781 gaaatcgacg gaaaaaaagt caccgttcct gacggatgga atgtgatcca ggcggccgag
      841 aagcttgaca tcgaggtgcc gcattattgt tatcaccctg ggctgagtat ctctggtaac
      901 tgtcgcatgt gcttggtcga aatcgaaaag atgcccaagc tgcagatcgc ttgcaacacc
      961 aaggtgaccg aagggatggt tgtcaaaacg gcaagcccga aggcgaaagc tgcccagacc
     1021 gcagtcttgg aattcattct cgtgaatcac ccgatcgact gcccgatttg cgatcaggct
     1081 ggtgagtgca agttgcaaga ctattacatg gattatggta agcatcacag ccaggttccc
     1141 ttggaggcga aagtcgagaa gaagaaagtc gtcgacatcg ggccgcaagt ggtgctcgac
     1201 caggagcggt gcattctctg cgcccgctgc acccgcttcc ttgatgaagt gacgaaaacg
     1261 ggtgagctcg gactctttgc gcgcggcgat cacacctata tcgatctttt ccccggcaag
     1321 agactcgaga atccctattc cgcgaatgtg gtcgatgtct gtccggtggg cgcgctgacg
     1381 cacaaagatt tccgtttcaa agcgcgcgtg tggtacctcg aaaaagttga gtcggtgtgc
     1441 acaaagtgtg caaatgggtg caacatcgat atctaccacc gccgcggaca aatgtttcgt
     1501 tttcgtccac gttccaatcc ggatgtgaat cagtattgga tgtgcgacga aggccggctc
     1561 tggtacaagg aattgcagaa agaaagccgt atcctccgcc ccgtggcgcg ggaggatgat
     1621 gctttcatgc ccatctcctg ggatcaagcc gtaggc
//
LOCUS       DASTWE010000069         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_551191, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000069 DASTWE010000000
VERSION     DASTWE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551191"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFT30_00755"
     CDS             complement(<1..452)
                     /locus_tag="VFT30_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091180.1"
                     /translation="MKRSVVLVAILGLFTMASFTFADDGTTPTTPTPALDVVTLKDGS
                     VIYGEVIEMTGGLLHIKNSMVNDIIKVKWEAVSKLAVSHPTPFHLKDGTVLIGTVEES
                     EPEMMKLKAGPTGSNMTVPMNVVTQVNPVVQSPVVYTGSINAGYSQSAG"
     gene            complement(576..1382)
                     /locus_tag="VFT30_00760"
     CDS             complement(576..1382)
                     /locus_tag="VFT30_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU5091181.1"
                     /translation="MTVVDTLTQYAVQYGLQAAVALGIFIVGAMVSRWAGNFAQQALE
                     RQTLEPPVRLLLVRVVKIVVMLFTAMIALQTLGVPIAPLIAGVGVAGVGIGLALQGVL
                     SNVMAGLSIIFSKPYKVGEHISLLGVHGDVVVIDIFTTTLVHADRSRVIIPNRKIVGE
                     ILHNFGTIRQMHLTIPVSPNANLDEALAQVRDVLDRHPRVMKDPVPSVGVASLGESPL
                     AVAIAIEPWTAAADYSSTQGELNKLILQRFQERGIALPSASLTVHLVNDR"
     gene            complement(1472..>2180)
                     /locus_tag="VFT30_00765"
     CDS             complement(1472..>2180)
                     /locus_tag="VFT30_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU5091182.1"
                     /translation="AGSDAPPFKGGVTVQGVQLGPALNAVAETPISISGTAGADLSLQ
                     GKGFSMPDLTKALEGTGHMAVKDGKIEGVNLLQEVASALKVPGISFDNAKATAFSTIE
                     TDLAIKQGIINVQRLLMDSHDFQATGGGTIGFDQRLNLAVNLNLSQDASQKLATASPV
                     VKIAMKDGRLSFPLTITGTAQAPSYGVDLKGVTGRVHQQVQKKVEEAVDGLIKGTKTP
                     EDLKKEGQELLKGLFGR"
BASE COUNT          490 a          613 c          552 g          525 t
ORIGIN      
        1 cccgctgatt gtgagtatcc ggcattgata ctgcctgtgt aaacgacagg cgactggacg
       61 acggggttga cttgcgtcac cacattcatc ggcaccgtca tattacttcc ggtcggaccg
      121 gccttcagct tcatcatttc cggctcactc tcctcgaccg taccgatcag aacagttcca
      181 tccttcaaat gaaagggagt cgggtgagag acggccagtt tactgaccgc ttcccacttg
      241 accttgataa tgtcattcac catggagttt ttaatatgga gcagcccacc cgtcatctct
      301 atcacttcgc cataaatcac actgccgtct ttcaaggtca cgacatccaa ggccggagtc
      361 ggcgttgtgg gagtcgttcc atcgtccgca aaagtaaagg acgccatcgt gaacagacca
      421 aggattgcga ccaacaccac agaccgtttc atttattctc cttagtatag ctaagtactt
      481 atctacagtt gttcctaggt atgtaccgtg tttgcgcagg gcagcgcaat acactcatgc
      541 cattcactcc cttgttgcgg gtttagtggg tagggctaac gatcgttcac taagtggacg
      601 gtaaggcttg cagagggcag tgcgatccct cgttcttgaa atcgttgaag aattaatttg
      661 ttcaactctc cttgagtcga gctgtaatcc gccgcagccg tccatggctc tattgcaata
      721 gccacagcca gaggcgattc tcccagagat gccactccca ctgaaggaac cggatctttc
      781 atgacccttg gatgtcgatc gaggacatcc cgaacttgag caagtgcctc atcgagattc
      841 gcattcggtg acacgggaat ggtcaaatgc atctgacgga ttgttccaaa gttgtgtaga
      901 atctctccga cgatttttcg attggggatg atgacgcggg agcgatccgc atgcaccagc
      961 gtcgtcgtga agatatcgat caccacgaca tcgccatgga cgccgagcaa cgaaatatgt
     1021 tcgccgacct tgtagggttt gctgaaaatg atcgagagcc cggccatcac attgctcagc
     1081 acaccctgca acgctagacc gatgcccacg ccggcaactc cgacgcctgc gatcagcgga
     1141 gcgatgggca cgccgagcgt ctgaagagcg atcattgcgg taaagagcat caccacgatt
     1201 ttgacgacgc ggaccagaag taggcgaacc ggtggctcaa gggtctgccg ttcgagcgct
     1261 tgctgtgcaa aattccctgc ccagcgagac accatggcac cgacgatgaa gatcccaagc
     1321 gcaaccgcgg cctgcagtcc gtactgcacg gcatattgag tgagcgtatc aacgacagtc
     1381 atagggttaa tctcctctag aggacacaca tctctgacac gttgaatcct gagcaatacg
     1441 agaacgcgag gggtgggctt tcaatggtcc gctatcgtcc gaagagtcct ttcagaagtt
     1501 cctggccctc tttcttcaag tcctccggtg ttttcgtacc tttgatcaat ccatcgacag
     1561 cttcttccac cttcttctgt acctgttgat gcactctccc cgttacacct tttagatcaa
     1621 ctccatacga cggtgcctgc gcggtaccgg taatcgtgag cgggaaactc aaccggccat
     1681 ctttcatcgc gattttgacg accggtgagg cagtcgcaag cttttgactc gcgtcttgcg
     1741 acagattgag attcacggca agattcagtc gttgatcgaa tccgatcgtg ccgccaccgg
     1801 tggcttgaaa atcatggcta tccattaaca gacgctgcac gttgatgatc ccttgcttga
     1861 tggccagatc cgtttcgatc gttgaaaatg cggtcgcttt agcattgtcg aatgaaatgc
     1921 cgggtacctt gagagccgat gctacttcct ggagcagatt caccccttcg atcttcccat
     1981 ctttcaccgc catgtgcccg gttccttcca atgcttttgt caggtcaggc atggagaagc
     2041 ccttgccttg cagtgaaaga tcggcgccgg ctgtgccgct gatggagatc ggtgtctccg
     2101 cgacagcatt gagcgccgga ccgagctgca ctccttgaac agtgacgcca cccttgaaag
     2161 gtggcgcatc ggacccagcg
//
LOCUS       DASTWE010000070         1438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_518851, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000070 DASTWE010000000
VERSION     DASTWE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518851"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..853
                     /locus_tag="VFT30_00770"
     CDS             161..853
                     /locus_tag="VFT30_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091183.1"
                     /translation="MKIFSLVLMFAVSLVQARASDVYGTVPRAEASRYPASAQQEQLG
                     IGAQWLTAEQTRRAFAMELHPTVLVVEVAIFPGRGTPINISRSDFAVRNVGSEKLSKP
                     LHPRAVAMTVERRSGAGVDAETYDQVGIGIERGERIDPATGQRERVGGVYTSTGTGVT
                     VRSKGGQPTERELEAIERELTDKGLPEGAVSRAVAGYLFFQFPDKKKVGPYDLECRLE
                     GTKLALGLKPSK"
     gene            complement(959..>1438)
                     /locus_tag="VFT30_00775"
     CDS             complement(959..>1438)
                     /locus_tag="VFT30_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091184.1"
                     /translation="GVANAILGGWQLGGILTLQDGFPITALCGPGTIQNGGGVFYPNA
                     TGISPNLKRSEKKRERFFNTDAFVDLAPGRPQFVFGNAARNTIIGPGIISFDASANKK
                     FSFGESRYLEFRTEFFNMPNHPIFNPPGRDLRTTNYGVITSTKIDSRQIQFGLKLVF"
BASE COUNT          325 a          387 c          430 g          296 t
ORIGIN      
        1 gggttcagga tttgagatcc atgtcgctgg agcccggtgt ttacaatctc aggatcgaga
       61 cggaaggacg tccgggtttt gcgcgccgag tttacgtact cagtggaaaa acactcagcg
      121 tcgaggccaa cctgatggcg ccgaaagggg agcaacagcc atgaaaatct tcagtctcgt
      181 gctgatgttt gctgtttctt tggtccaggc ccgtgcgagc gacgtgtatg gcacagtgcc
      241 ccgagcggag gcttctcgat atcccgcgag cgctcaacaa gagcaactcg gtatcggagc
      301 acagtggctc accgccgagc agactcgtcg ggcctttgcc atggaactcc atccgaccgt
      361 gctcgtggtt gaggtcgcga tatttccggg aagagggaca cccatcaaca tctcgcgttc
      421 agactttgca gttcgcaatg ttggcagtga gaaactatcg aaaccgctac atcctcgggc
      481 cgtagcgatg acagtagaac gacgctctgg tgcaggcgtc gacgccgaaa cgtacgacca
      541 agttgggatc gggattgaga gaggtgagcg gatcgacccc gccacgggcc agcgagagag
      601 agtcggcggc gtctacacct cgaccgggac aggtgtgacc gttcgcagca agggtgggca
      661 accgacagaa agggagctcg aagcgatcga acgtgagctc actgacaaag gtttgcctga
      721 aggagctgtg agcagagctg tggccggtta cttattcttt caattcccag acaaaaagaa
      781 ggtcggccct tatgacctgg agtgcagact cgagggaacg aagctagcgt taggcttgaa
      841 gccttcgaaa taggcagacc tgggtacccg aagcgtcagc cggcgatagg tcaggtgggg
      901 tggggcggac cgtgcgtccg cccatttgtc gtttggctcg tcaccggtaa gccctgactc
      961 aaaacaccaa cttcaaccca aactgaatct gccgggagtc gatcttggtg cttgtgatca
     1021 cgccatagtt cgttgttcgc aggtctcggc ctggcggatt gaagatagga tgattgggca
     1081 tgttgaaaaa ctctgtgcgg aactcgagat agcgtgattc accgaagctg aacttcttgt
     1141 tcgccgaagc gtcgaagctg atgatgcccg gcccgataat cgtgtttcgc gccgcattgc
     1201 caaacacgaa ctgcggcctg cccggtgcca gatccacgaa agcatccgtg ttaaagaacc
     1261 gttcacgctt cttctcgctt cgcttcaagt tcgggctgat gccggttgcg ttcgggtaga
     1321 aaacaccgcc gccattctga atggtgcctg gaccgcacaa tgcggtaatc gggaagccat
     1381 cctgcaatgt cagaatccct ccgagttgcc acccaccgag aatcgcattc gcaacacc
//
LOCUS       DASTWE010000071         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1359084, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000071 DASTWE010000000
VERSION     DASTWE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359084"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFT30_00780"
     CDS             <1..602
                     /locus_tag="VFT30_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091185.1"
                     /translation="VPVIVFTGKELTDEEELRLLDLSQSIVLKGVQSPERLLDEATLF
                     LHLLTAQLPPEKKTMLQRVRQSDDSLIGKQILVVDDDVRNIFALTSLLELHGMAVTSC
                     ETGREAIELVESDSDLDMVLMDIMMPEMDGYETIRRIRSNPAHRLLPILALTAKAMKG
                     DRERCLQAGASDYIAKPVNTEELLALMRIWLFGKKKVRR"
     gene            608..>1091
                     /locus_tag="VFT30_00785"
     CDS             608..>1091
                     /locus_tag="VFT30_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091186.1"
                     /translation="MSETSVNVSKKMPWSMDMADSSALTEIPAHILLVDDDPGILSAM
                     ASLLEGPGRTVLTAKTGQSALRHLLRHDCAVIVLDVRMPQMDGFELATLIRQRDRSRF
                     TPIIFLSGVDTMDADVVRGLSSGAVDYLVKPVLPDVLQHKVDAFVELFRLREWAQQQA
                     R"
BASE COUNT          229 a          298 c          333 g          231 t
ORIGIN      
        1 atgtgccggt catcgtgttt accggaaagg aattgaccga cgaagaggaa ttgcggcttc
       61 tggacttgtc ccaaagcatt gtactcaaag gcgttcagtc gccggaacgg ttgctggatg
      121 aagcgacgct cttcctccat ctccttacgg cgcagttgcc gccggagaag aagacgatgc
      181 ttcaacgggt gcggcagagc gacgactcgc tcatcgggaa gcaaatcttg gtcgtggacg
      241 acgatgtccg caacatcttc gcgctcacga gcttgctcga gctgcacggc atggccgtca
      301 cgagttgcga aacgggccgg gaagcgatcg aactggtcga gtcggattcg gatctcgaca
      361 tggtgctcat ggatatcatg atgccggaga tggacggata tgaaacgatc cgtcggattc
      421 gcagcaatcc ggcgcatcgc ttgcttccga ttttggcgtt gacggcgaag gccatgaagg
      481 gagaccggga gcggtgcctg caggcaggag cgtccgatta catcgccaaa cccgtcaaca
      541 cggaagaact gctcgccctc atgcgcatct ggctatttgg taagaagaaa gtgaggcgtt
      601 gacaatcgtg tccgagacat cggtgaacgt gtccaagaaa atgccctggt ccatggatat
      661 ggcggacagc tccgcgctaa ccgagattcc cgcgcatatt ctgctcgtcg atgacgaccc
      721 aggtattctc agcgctatgg cgtctctcct agagggcccc ggtcgcacag tcctgacggc
      781 caaaacaggc cagagcgcgt tgcggcatct gctgcgccat gattgcgcag tcattgttct
      841 cgatgttcgg atgccacaga tggatggttt tgaattggcc acattgatcc ggcaacggga
      901 ccgttcccgc ttcacgccca tcattttcct gtcgggcgtc gacaccatgg acgcggacgt
      961 ggtgcgaggg ctttcgagcg gagctgtgga ttacttggtc aaaccggtcc tgcccgacgt
     1021 tcttcagcac aaagtggacg cattcgtgga attgtttcgg cttcgcgagt gggcccagca
     1081 acaggcccgg c
//
LOCUS       DASTWE010000072         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1326720, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000072 DASTWE010000000
VERSION     DASTWE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326720"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFT30_00790"
     CDS             <1..1133
                     /locus_tag="VFT30_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091187.1"
                     /translation="LHVEEDRLMELLTLNAQYQPAKAIENWDSLLWVERFNTISDFKL
                     ETGMVSEYMSILPEGTVLTLRESNVPMIVETHQIERKKNTPQKLIITGRSFTSILDRR
                     AAIQSVAALTGSANWNVNVKTPSDLAYYIINKICVEGQVSAADIFPAAQVQFPTPADY
                     LTSTGPTKSFSVDRGNLLEIVLKLLQTEAAADPTTTPATPAIAQHGIRAVRPNQNGTA
                     IAIEIYTGVDRRSTVYFDVTRDLLDDGNYLFSKVGSKNAAYGVAAGLAATMYEGAVEP
                     TGLNRRVVLVDASTSGIADPTILKNAMSQSLAEASETAMFDGSINQELSPYIYGVDYG
                     LGDIVRTVGDYGLDQASRVTEFIRSEDKSGRKAYPTLQALTV"
     gene            1142..1333
                     /locus_tag="VFT30_00795"
     CDS             1142..1333
                     /locus_tag="VFT30_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091188.1"
                     /translation="MESTNPLQGLLPARVRQILYAILFVAGLVFALWQAAEGDWLLFI
                     GSLFPALLGLLAAGNTTST"
     gene            1390..1695
                     /locus_tag="VFT30_00800"
     CDS             1390..1695
                     /locus_tag="VFT30_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091189.1"
                     /translation="MRNPFKRKPKPYIEERERILSVMETVSPYSDEYERLMKRLNELD
                     RLINRTSELKKTVIPALGTVGAITGIYALQQFAGVIVPKALEAHAARQEQKKSQQDD"
     gene            1843..2067
                     /locus_tag="VFT30_00805"
     CDS             1843..2067
                     /locus_tag="VFT30_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091190.1"
                     /translation="MPLINRKQKVAKIRPADIIVIGGRKFIVRRNEIASLVFQHMRCL
                     TVVPLNKREQEFLTTLTVSDTEKFKITRHK"
BASE COUNT          522 a          639 c          586 g          454 t
ORIGIN      
        1 ctctacatgt ggaggaggac cgcctgatgg aactcctgac actgaacgcc caataccagc
       61 cggcgaaggc gattgagaac tgggatagtc tcctatgggt cgagcggttc aacaccatca
      121 gcgacttcaa actcgagact ggaatggtct ctgagtacat gtcgattctc cccgagggga
      181 ccgtattgac tcttcgtgag tcgaacgtgc ccatgatcgt ggaaacgcat cagatcgagc
      241 ggaagaagaa cacaccgcag aagctgatca tcactggtcg ctcgttcacg tctatattgg
      301 accgacgtgc cgccattcag tccgttgctg ctctcaccgg gtctgccaac tggaatgtga
      361 acgtgaagac gcccagtgat ctggcgtact acatcatcaa caagatctgt gtcgaaggcc
      421 aggtttcggc tgccgatatt ttccccgctg ctcaggtgca gttccccaca ccagcggatt
      481 acctcacatc gactggaccg acgaagtcgt tctcagtgga tcgaggcaac cttctcgaaa
      541 tcgtcctcaa gttgcttcag accgaagcag cagccgatcc gaccacaacg ccggctacgc
      601 ccgctatcgc acagcatggt attcgggccg tacgaccgaa ccagaacggt actgccatcg
      661 ccatcgagat ctacaccggt gtcgatcgac gcagtaccgt atatttcgat gtcacgcgcg
      721 atttgttgga tgatggtaac tacctcttca gcaaggtcgg atccaagaat gctgcatacg
      781 gcgtggccgc cggtttggct gcaacaatgt atgaaggggc ggtggaaccc actggactca
      841 atcgtcgggt ggtgcttgtc gatgcgtcga cgtctggcat tgctgatcca acgattctca
      901 agaacgccat gagtcagtca ttggccgagg cgagcgagac agccatgttc gatggctcca
      961 tcaaccaaga gctgagtccc tatatttacg gtgtcgacta cggtttgggc gacattgtga
     1021 gaaccgtagg tgactacggc cttgatcagg cctctagggt gactgagttc atccgatccg
     1081 aggacaagtc tggccgcaag gcatatccca cactccaagc tctgactgtc taggaggaca
     1141 gatggaatcc acgaatcccc tgcaggggct actcccagcg cgtgtgcgcc aaatcctgta
     1201 cgcaatcctc ttcgtcgccg gcctggtctt cgccttgtgg caggccgccg agggcgactg
     1261 gctgctgttc atcggcagcc tcttcccggc gctgcttggg cttctggccg caggcaacac
     1321 cacgtcaacc tagcgcacga aaaacatctg ttaaggtgca gaacccatct caccttacaa
     1381 ggagttccaa tgcgcaatcc cttcaagcgg aagcccaagc cctacatcga ggagcgcgag
     1441 cgaatcctga gcgtcatgga aaccgtgtca ccgtactccg atgagtacga gaggctgatg
     1501 aagcgtctga acgaactcga ccgtctgatc aaccggacga gcgagctcaa gaagaccgtc
     1561 atcccagctc tcggaaccgt cggagcgatc accggaatct atgcgctcca gcagttcgct
     1621 ggcgtcatcg taccgaaggc gcttgaggcc cacgccgcaa ggcaggagca gaagaagtcc
     1681 cagcaggacg actgattctg aaaggcgtaa gtcactggaa acagtggctt atgtcttctc
     1741 aaaaaatccc gggtgggaaa atcgatttca aagtgaaacg tggggctggt gtgatttaca
     1801 taccctataa tgaccgaacc tacactcaca ctggagaatc aaatgccact catcaaccgc
     1861 aagcagaagg tcgccaagat ccgccccgct gacatcatcg tcatcggcgg taggaagttc
     1921 attgttcgga ggaacgagat cgcaagtctc gtcttccagc acatgcgctg cctcaccgtc
     1981 gtgccgctga acaagcgcga gcaggagttc ttgacgactc tcactgtctc ggacaccgag
     2041 aagttcaaga tcacccggca caagtagtcc gtcaagcccg aagccagcca catcggccta
     2101 gggttttcat atttacatgc cctatgatga ccactcaacc taaggagaaa ctcatgctca
     2161 agttcgcact cggtttgacc accggccttg ctctcggtgg t
//
LOCUS       DASTWE010000073         1010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_938986, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000073 DASTWE010000000
VERSION     DASTWE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938986"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFT30_00810"
     CDS             <1..827
                     /locus_tag="VFT30_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU5091191.1"
                     /translation="NFTSGFLPSQHAGTPFRSQGDPILYLSNPKGVSNEAQRLRLDLI
                     RDMNQDRQAQTGDVEIASRIHSYELAYKLQSAGPELLDFSKESPATLESYGVNQEATK
                     AFGTNCLLARRMVERGVRYVMLMHASWDHHSGIVSGLKRNCGATDQPSAALLKDLKQR
                     GLLEDTLVVWGGEFGRTPMSEIRRPDDPNSIGRDHHPNGFSLWMAGGGIKGGQVIGET
                     DDLGLEIVSDRVHVHDLQATILHCLGLDHKRLTYRHLGRDFRLTDVGGEVVTKMLA"
BASE COUNT          217 a          274 c          305 g          214 t
ORIGIN      
        1 ataacttcac cagtggtttc ctgccatcac aacatgcagg cacgccattt cgcagtcagg
       61 gcgatccgat tctgtaccta tcgaatccga agggagtgtc gaacgaagcg cagcgtctac
      121 gactggacct gattcgtgac atgaatcagg atcgtcaagc gcaaaccggc gatgtcgaga
      181 tcgcatcgcg cattcattcc tatgaactcg cgtataagct tcaatctgca ggtcccgagt
      241 tgctcgactt ctccaaagag tctcctgcga cgctcgagtc ctacggggtg aatcaggagg
      301 cgacgaaggc gtttggtaca aattgcctgc tcgctcgccg catggttgag cggggtgtcc
      361 gatacgtgat gctgatgcac gcgagctggg atcaccacag cggcattgtt tcggggttga
      421 agcggaattg tggcgccacc gatcagccga gcgccgcgct cttgaaggac ttgaagcagc
      481 gaggactgtt ggaggacacg ttggttgtct ggggcggcga gttcggcagg actccgatgt
      541 cggagatccg gcggcctgac gatccgaaca gtatcggacg agaccatcat ccgaacggct
      601 tcagtctctg gatggccggt ggcggcatca aaggcgggca agttatcggc gaaactgacg
      661 atctcggact agagatcgtc agtgaccggg tacatgtcca cgatctacag gcaacaatcc
      721 ttcattgcct tggcttggac cacaagcggc ttacttaccg tcacctcgga cgcgacttcc
      781 ggctcaccga tgtgggtgga gaagtcgtta cgaagatgct cgcgtagctg ttcgccgcga
      841 ccgtcaggcc ttcgtacact cgaagtcgat ggttcggtcg aatggtgtca gaccggtcag
      901 atacacacta ggcccaggta gcttgccttc cgctccacga acttgggtat acgtgtggtc
      961 ggtgacgcca ggtccaaagc ggataatgtc cgagcccatc cggtaagagg
//
LOCUS       DASTWE010000074         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_842053, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000074 DASTWE010000000
VERSION     DASTWE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842053"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFT30_00815"
     CDS             complement(<1..525)
                     /locus_tag="VFT30_00815"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU5091192.1"
                     /translation="MALIVQKYGGTSVGTIERIHRVADRVAHTQGAGNQVVVVLSAMS
                     GETDRLVKLAHEVTTVPDERELDMLLSTGERITIALLSMELRGRGINARSFTGRQVGI
                     ITDSAHTKARIARVTADRIKEALKQGVIPVVAGFQGINEQSDVTTLGRGGSDLSAVAL
                     AAALKADRCVIFTDV"
     gene            complement(530..1738)
                     /gene="apgM"
                     /locus_tag="VFT30_00820"
     CDS             complement(530..1738)
                     /gene="apgM"
                     /locus_tag="VFT30_00820"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU5091193.1"
                     /translation="MKYVIVHAGGMADHPQPELGGRTPLQASSTPNLDRLAQIGELGR
                     LLTPMDGVRHGSGLIGAAILGYDPKKFYPGPGPCEAARVGVSVSEHAVVYRCTMVTLR
                     PESGKGGEIKKLGAHVIMDDATAGLIETEEARELIEAINEQLGSETIQFYPGAGHRHL
                     MVWVNGKPRAICNDPQTILGQSIADALPTGDGADILRKLMDAAHLILRDHPVNDDRLA
                     GGKKPANCVWLWGEGRAVMWPSLVEKYDIAGAVVATSDVYRGVGISAGLEAVDPDRLA
                     DGDLRTKATVALEELAKKDFVYVHAELTDEVIHGTDIKAKVHGIEEFDRNLVGPLVEG
                     LAKQGPYRFLIICDHAEDPQSPAFYTFGEGGITGSETVGRRFTEADALATNMPTRDAT
                     KFVNKFFSKS"
     gene            complement(1755..>2185)
                     /locus_tag="VFT30_00825"
     CDS             complement(1755..>2185)
                     /locus_tag="VFT30_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5091194.1"
                     /translation="MRVPRMMGFQAAGAAPIVLGRIVEQPQTIATAIRIGNPASWSTA
                     LKVVEESAGAIDMVTDDEILQAYTTVAATEGVFCEPASAASVAGVAKLHRAKALREGE
                     TVVCTLTGHGLKDADTAISVSKQPQTVKATREDVARLLNV"
BASE COUNT          436 a          702 c          554 g          493 t
ORIGIN      
        1 gacgtcggtg aagatgacgc accgatccgc cttcaaggct gccgccagcg caacggcaga
       61 gagatctgat cccccacggc cgagggtggt cacgtcagat tgttcattga tcccctggaa
      121 tccggccacg accggaatga cgccctgctt caaggcctcc ttgatgcgat ccgccgtgac
      181 ccgtgcgatc cgtgctttgg tatgggcact atcggtaatg atgccgacct ggcgtcccgt
      241 aaaggaccgg gcattgatcc ctcggccccg caattccatc gacagcagcg cgatggtaat
      301 ccgttcccct gtcgagagca gcatgtccaa ttcgcgttcg tccggaacag tagtcacttc
      361 atgcgcaagt ttgaccagcc gatccgtctc cccactcatc gccgagagta cgacgacgac
      421 ctgattgccc gccccttgcg tgtgggccac ccggtcggca acgcggtgga tccgctcgat
      481 cgttccaacg gaagtccctc cgtatttttg aacgatcagc gccatgggat cagctttttg
      541 agaagaactt gttcacaaac ttggtggcat cacgggtagg catgtttgtg gcgagcgcat
      601 cggcctcggt gaaacggcga ccgacggtct ccgatcctgt gattcctcct tcaccaaacg
      661 tatagaatgc cgggctctgc ggatcttccg cgtgatcgca aatgatgagg aagcgatagg
      721 gaccttgttt ggctaaaccc tcgactaacg gtccgactaa gttgcgatca aactcttcaa
      781 tgccatggac tttggccttg atatcagtcc cgtggatcac ctcgtccgtc aattcagcat
      841 gcacatacac aaaatctttc ttggccaatt cctccaatgc caccgtcgcc ttggtgcgga
      901 gatcgccgtc ggccagccta tcgggatcca ccgcttcgag accggcggag attccaacac
      961 cacgatagac gtcgctcgtc gcaacgactg cccctgcgat gtcatatttt tctaccaagc
     1021 tcggccacat gaccgcccgc ccttcacccc acagccagac acagtttgcc ggtttctttc
     1081 cccctgcgag ccggtcgtca ttcaccggat ggtcgcgcag tatcaggtgc gcggcatcca
     1141 tcaacttccg gagaatatcg gcaccatctc ccgtgggaag ggcatccgca atggattgac
     1201 cgaggatcgt ctgaggatca ttacagatgg ctcgcggttt tccattcacc cataccatca
     1261 gatgacgatg ccccgcccca ggatagaatt ggatggtttc tgaaccgagc tgctcattga
     1321 tcgcttcgat caattcacgc gcttcttcag tttcgatcaa cccggccgtc gcatcatcca
     1381 tgatcacgtg cgcccctaat ttcttgattt caccgccctt gccactttcc ggtcgcaggg
     1441 tcaccatcgt acagcgatac accacggcat gttctgatac cgacaccccc acccttgccg
     1501 cctcacatgg cccgggaccc gggtaaaact ttttcgggtc atacccaaga atcgccgcgc
     1561 cgatcaatcc gctcccatga cgaactccgt ccataggagt cagcagtcta ccgagctctc
     1621 cgatttgcgc caggcgatcg agattgggcg tcgatgacgc ttgtagtggt gtacgtccac
     1681 ccagttccgg ttggggatgg tccgccattc ctccagcatg cacgatcaca tatttcatga
     1741 gatccacgcg tctttcatac gtttaataat cgggcaacgt cctcgcgcgt cgctttgacg
     1801 gtttgtggtt gcttcgacac actgatggcc gtatcggcat cttttaaacc atgccctgtc
     1861 agcgtgcata ccaccgtctc cccttcccgc agagccttgg ctcgatgcag cttggctacg
     1921 ccggcgacgg acgcggcaga agccggctca caaaagaccc cttccgtcgc cgccactgtt
     1981 gtgtaggcct ggagaatctc atcatccgtc accatatcga tcgcgcctgc ggattcctcc
     2041 acaaccttca aggccgtaga ccaactggcg ggattgccga ttcggatggc tgtggcgatc
     2101 gtctgcggtt gttcgacgat cctgcccaac acaatcggag cggcacctgc ggcttgaaag
     2161 cccatcatgc gaggcactct catcg
//
LOCUS       DASTWE010000075         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1423846, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000075 DASTWE010000000
VERSION     DASTWE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423846"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFT30_00830"
     CDS             complement(<1..260)
                     /locus_tag="VFT30_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091195.1"
                     /translation="MAISEAYPNGRWHPYVGVGVGMHQMAMRPGGFAGASSCCVPGGA
                     LDTNVITDQRTTTIGFQGVGGVKAHLFKYVAAFIEAKYLHAH"
     gene            complement(345..728)
                     /locus_tag="VFT30_00835"
     CDS             complement(345..728)
                     /locus_tag="VFT30_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091196.1"
                     /translation="MKARISAIVCLLCVLGSASLTQAEDPYSQRVADPYAISPDPPTM
                     SSGTLEPYMSFFAGVGIPFSQDATFTDGTQPTVVNDIDYTSKFSLGGNLGVWFPTRNK
                     LWGFDLGVELTGYLWYPDVRCCRDN"
     gene            complement(1277..>1565)
                     /locus_tag="VFT30_00840"
     CDS             complement(1277..>1565)
                     /locus_tag="VFT30_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5091197.1"
                     /translation="EKDPETQAIVMIGEIGGDAEEKAAEFIKRNVKKPVISFIAGITA
                     PPGRRMGHAGAIISGGKGTAAEKMKTLEAAGVKVVKNPAEIGAAVKQALGR"
BASE COUNT          355 a          471 c          411 g          328 t
ORIGIN      
        1 tgatgggcat ggagatactt cgcctctata aacgccgcca cgtacttgaa aagatgcgcc
       61 ttgactccgc ccacgccctg aaatccgatc gtcgtagtcc gttggtcggt gataacgttc
      121 gtatctaaag caccccccgg cacacaacac gaactagccc ccgcgaatcc acctggcctc
      181 atcgccatct gatgcatgcc cacgccgact ccgacgtacg gatgccaccg tccgttggga
      241 taggcctcgg aaatcgccat gggatagcga atcaggaaat tggggccgat gtagaggcct
      301 tggatctccg tcccggtgcc gctacccgtt gtgggatcac gattctaatt gtcccgacag
      361 cagcgcacat ccggatacca aagatagccg gtgagttcca caccaagatc gaatccccac
      421 aatttgtttc tggttggaaa ccagaccccc aggttgcccc cgagcgagaa tttcgacgta
      481 tagtcaatat cgttgaccac cgtcggctgg gtcccgtccg taaacgtggc gtcctgactg
      541 aacggaattc caacacccgc aaaaaaggac atatagggct cgagcgttcc ggaggacatg
      601 gtcgggggat caggcgagat cgcataggga tcggcaaccc gttggctgta cggatcttcc
      661 gcctgggtga gactcgcaga gccgaggaca cacagaaggc aaacgatcgc cgaaattctc
      721 gctttcattc agcgtcctcc cacggtaaag aaaccatgtg cttcgacaaa tggggtcgcg
      781 caatctgaga ttcgtcagtc tctcgtcggt cagcgaggcg atctgagacg agtgcgtctg
      841 cgtgagactc gactcgatcc ccggttcggc tggtgtggtg tattgacata tgtgtccacc
      901 tatccacctc cagagcgatc aggaggggat gctctgctcg catcgtaggg ggcggacggc
      961 gcccacagta acaaccccac gttcagtttt gaaaatagat aaaacggatt ggagttatcg
     1021 gtaaatccaa tatcccaccg gacatgcgga cagggctgtg gcgctggaga aatcgactct
     1081 gcctccaacc tgcactcgcc atcatcaccc gtcgcgcttc tctatggcac tgacacatac
     1141 gcgcaaacaa ctcattgaaa gatatgacca tcgtcacgcc cgcgtcactg agcagaggcg
     1201 atggacatcc catcgatgtg acaccgacga agttgtgaga gagaacgctg ccaaccggaa
     1261 gagcaagcta agagtactac cgtcccaatg cttgtttgac cgccgcaccg atttcagccg
     1321 gattcttgac gactttgacc ccagccgctt ccagggtttt catcttttcg gctgcggtcc
     1381 ccttgccgcc ggagataatg gcgccggcgt gacccatgcg gcgtccagga ggcgccgtga
     1441 taccggcaat gaagctgatc accggcttct tgacgttcct cttgataaac tctgccgctt
     1501 tctcctccgc atcaccgccg atctccccaa tcatgacgat cgcctgagtt tcagggtcct
     1561 tttca
//
LOCUS       DASTWE010000076         1213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_260527, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000076 DASTWE010000000
VERSION     DASTWE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260527"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFT30_00845"
     CDS             complement(<1..744)
                     /locus_tag="VFT30_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5091198.1"
                     /translation="MTPDHAEIMRRVADIAPRLAERAVACDTERQVVSESMREMINAG
                     LFRIPQPARVGGYELSLRTLGAAVTAVSSACSSSGWVLMVMGAHHWCVGTFPQQAQEA
                     IFGDGRDGLVAGTLSWQGNAVPVGGGYRVNGRWQFCSGVDHADWVMLGCADPESHNPR
                     VHVVVPVGDLEIDDTWHVMGLQGTGSKDVVAHDLFVPEHHTIDTRQMFGGTSPYITNH
                     RSNLYHLSAEAMLCMSVSTSVLGSAQFALD"
     gene            908..>1213
                     /locus_tag="VFT30_00850"
     CDS             908..>1213
                     /locus_tag="VFT30_00850"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEU5091199.1"
                     /translation="MSLYAQQVDTGVTRVQSAEEMSPEERAFQERIDAGIKIEPKDWM
                     PEAYRKTLIRQISQHAHSEIVGQLPEGNWITRAPTLKRKTVLLAKVQDEAGHGLYLYG
                     "
BASE COUNT          302 a          367 c          315 g          229 t
ORIGIN      
        1 gtccaacgcg aactgcgcag aaccgagcac cgaagtggat accgacatgc agagcatcgc
       61 ctcagccgat aagtggtaca aattactgcg atgattggta atgtaaggac tcgtaccgcc
      121 aaacatctgc cgggtatcaa ttgtgtggtg ttcgggaacg aaaaggtcgt gcgccacgac
      181 gtctttgctt ccagtgccct ggagccccat cacgtgccag gtgtcatcga tctcaaggtc
      241 accgaccggg acaactacat ggacccgcgg attgtgcgat tctgggtcgg cacatcccag
      301 catcacccaa tcggcatgat cgacgcccga acagaactgc cagcgtccgt tgacacgata
      361 tccaccaccg actgggaccg cattcccttg ccaggataga gtgcctgcga ccagcccatc
      421 ccgcccgtcg ccgaaaatcg cctcttgtgc ctgttgcgga aacgtaccca cacaccaatg
      481 atgagcgccc attaccatga ggacccagcc actagacgaa cacgcactcg acaccgcagt
      541 gaccgcagcg ccgagcgtac gcagactcag ttcgtaaccg ccgacacgag ccggttgcgg
      601 aatacggaac aggcctgcat taatcatctc ccgcatgctc tcagatacaa cctgccgttc
      661 cgtgtcacag gcaaccgcac gctcggctaa cctcggcgca atgtccgcca ctcgtcgcat
      721 gatctctgca tgatcgggtg tcatatgtgt ccttccccgt aagtctctga ttcttgtcat
      781 tctagagaac ccaacgacag cgggaaagat caggcaggtc aaggcaagct cgattccagt
      841 atgccctgtc gcgtgctaca aaaagaaagg aattacctca agagtcctgc tctggaggag
      901 cgacgtcatg tcactatacg cgcaacaggt ggataccgga gtcacacgag tacagtcagc
      961 ggaagaaatg tcgcccgaag aacgagcgtt ccaagaacgg atcgatgccg ggatcaaaat
     1021 cgaacccaag gattggatgc ctgaggctta tcgcaagacg ctgattcgtc agatctcaca
     1081 gcacgcgcac tccgagatcg ttggacaatt accggaaggc aactggatta ctcgcgcgcc
     1141 gacgctcaag cgtaagaccg ttttgctcgc caaagtacag gatgaggccg ggcacggcct
     1201 ttatctttac ggt
//
LOCUS       DASTWE010000077         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_939098, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000077 DASTWE010000000
VERSION     DASTWE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939098"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFT30_00855"
     CDS             <1..248
                     /locus_tag="VFT30_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091200.1"
                     /translation="LSAGFVAWLLRGSSLLASFLASMPAWRHFDPLPVLGMGGGDRRK
                     LDRKIREEDEQEKREFRGLDRVLKASSPDPSSKKRGS"
     gene            262..>1130
                     /locus_tag="VFT30_00860"
     CDS             262..>1130
                     /locus_tag="VFT30_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5091201.1"
                     /translation="MRITPLVWISLGLVSLTLSVMLAGDSLVDLVPNHDRQVFEYRRD
                     LAESLAVQYSALAERGHMETIKFAMETLAKRNEDILSLALFQNGRGLIAQVGEHAQVW
                     VQPPGDESTFQFLQVPIFSDDQSWGVLQVAFRQTDVSIVERLLTDPWLRFLVFVSMVG
                     FIGYLIFMKRTLRQLDPSGIIPTRVKAALDALTQGVVMIDTRDLIVLANDAFSQAVGK
                     PVASLVGADLGSLSWKPLIPSASVSVHPWTRTIMDRQPLDNVPLLLTFPESEPRKFIV
                     NTVPIMDDGSAVRG"
BASE COUNT          224 a          306 c          329 g          271 t
ORIGIN      
        1 cgctatccgc agggttcgtg gcttggctcc tgcgcggcag ctctctgttg gcgagtttcc
       61 ttgcctcgat gccagcctgg agacattttg atccattgcc ggtgctagga atgggtgggg
      121 gtgatcgccg aaagctcgac cgtaagatcc gtgaggaaga tgagcaggag aagcgggaat
      181 tccgtggtct ggatcgggta ctcaaagctt cgtccccgga cccaagcagc aagaaacgag
      241 gctcatgagc aagacgaagt catgcgaata acacccctgg tctggatcag tcttggcctg
      301 gtgagcttga cgctcagtgt catgttagcc ggcgattcgc tcgtcgatct ggtgccgaac
      361 catgatcgcc aggtttttga gtatcgtcgc gacttggcgg aatcactggc agtccagtac
      421 tcagcgttgg ccgagcgggg tcacatggaa acgatcaaat ttgcgatgga gacgctggcc
      481 aagcgaaatg aggatatttt gtcgctggcg ctctttcaaa acggccgagg ccttatcgcg
      541 caagtcggcg agcatgcgca ggtatgggta cagccgcccg gcgatgaatc gacgttccaa
      601 ttcctccagg tccccatctt ttccgatgat cagtcatggg gtgtgctcca agttgcgttt
      661 cgtcaaaccg acgtgtccat cgtggaacgc cttcttaccg atccatggct gcgtttcctc
      721 gtgttcgtca gcatggtggg atttatcggt tacctgattt tcatgaaacg cacactccgc
      781 cagttggatc cctccggcat tattcccact cgtgtgaaag ctgctctcga tgcgttgact
      841 cagggcgtgg tcatgattga cacgcgagac ctgatcgttc tagccaatga tgcgttcagt
      901 caggctgtgg gcaagcccgt ggcatcattg gtcggagccg atctcgggtc cctctcctgg
      961 aaacccctta ttccatcagc ttcggtgtcg gtccatccat ggaccaggac gattatggac
     1021 cggcagcccc tggacaatgt cccccttctc ctgacttttc cggagagtga accgaggaaa
     1081 ttcatcgtca acaccgtgcc gattatggat gacggctcgg cggtaagagg
//
LOCUS       DASTWE010000078         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1488580, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000078 DASTWE010000000
VERSION     DASTWE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488580"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFT30_00865"
     CDS             complement(<1..948)
                     /locus_tag="VFT30_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEU5091202.1"
                     /translation="MFTRLATTLSVLILTGCLLAPSSADAVRIKDIGAIEGVRENQLL
                     GYGLVVGLDSTGDRVIGGQFTIQAMMSMLNKMGVNLVIDPIQLLTRNIASVMVTAKLP
                     PFSRPGMTLDAVVSSMANAKSLQGGTLLLTPLKAANQQVFAVAQGPVSIGGFLGGTGG
                     PGGATVAKNHQAAGLVPGGAIIEKELPVNIDSWDTVSVLLRQPDFTTAIRTAEAIESA
                     FGKGSASAVNAGSVKATIPQVYQGRVVEYIASIEGLDVTVDSVAKVVVNERTGTVVLG
                     EHVRISTCAISHGNLTISVKNTLNVSQPPAPLIGSSAGQT"
     gene            complement(1248..>2050)
                     /locus_tag="VFT30_00870"
     CDS             complement(1248..>2050)
                     /locus_tag="VFT30_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEU5091203.1"
                     /translation="PRLLGHWTLTNLRSKTREDLAWAKTYKKYLFSLRQVSRLLLRTS
                     VGFPVTALLLLQACSSPPSVSSKVVVSPLPPPKTVGSLWQEENGRAYLYEDLRAMRIG
                     DILTVRIVEKHKGSKSADTEAQRESTIKNSLVGSAMGYIGIPGIRFSDETRRGMGIDA
                     SANNKFGGKGATSREGTLTGTISTIVTEVLPNGDLRIEGRREVTVNSEKQLMSIGGIV
                     RRVDVDTKNTVLSSAIADARIEYSGLGVLDDVQRPGWFIRILDWVYPF"
BASE COUNT          435 a          603 c          534 g          478 t
ORIGIN      
        1 agtctgtccc gcggaagagc cgatcagcgg cgccggcggc tgcgacacgt tcaatgtgtt
       61 ttttaccgag atcgtgaggt taccgtgaga gatcgcgcat gttgaaatcc gtacatgttc
      121 tcccaacacc actgtcccag tccgttcatt gacgacgact ttggccacag aatcgacggt
      181 cacatccagc ccctcgatcg atgcaatata ttcgacgacg cggccctggt aaacttgagg
      241 aatcgtggct ttgacggatc cggcattcac ggctgatgca ctgccttttc cgaaagcact
      301 ctcgatggct tcggccgtcc tgatggcggt cgtgaaatca ggctgtcgta gaagtacgga
      361 taccgtatcc catgagtcga tgttgaccgg tagctctttc tcgatgatgg ctccaccagg
      421 aaccagaccg gccgcctgat gattcttggc caccgtcgcg ccgccaggtc ctcccgtccc
      481 gccgagaaac ccaccgattg agactggtcc ctgcgcgact gcaaacactt gctggttggc
      541 tgctttcaaa ggcgtgagca agagcgtacc gccttgcagg cttttggcat tcgccatcga
      601 cgatacaacc gcatccagcg tcatccccgg tcttgaaaat ggaggaagct tggctgtgac
      661 catcactgag gcaatgtttc tggtcaacaa ttgaatcggg tcgatcacga gattgacgcc
      721 catcttgttc aacatggaca tcattgcctg aatcgtgaac tgtccgccga tcactcggtc
      781 gccggtgctg tccaggccaa ccaccaaccc gtaaccgagc agctggtttt cgcgcacccc
      841 ttcaatcgcg ccgatatcct tgatcctcac cgcatcggca ctggagggag ccaacagaca
      901 ccctgtcagt atcagcacgc tcaaggttgt tgccagtcgt gtgaacatgc gtggatacct
      961 atcttcgcta cgaacgttta gacacggaca cctgagacct acgacccagt aggagacgcg
     1021 ccaaccatcc ctgctcagaa accacttctt tcaccgagtt acgatcaaca tcatccagca
     1081 tatggaatcg cgtcttgctc ccaaaggagg gaggatcaaa gtcagcccgt cgtacgactc
     1141 cactctgcac catcatctgt agggtgatta gattcaatgg gcgtcgcttg gaaatgcccc
     1201 tcctcgtagt cgtgcgcctc tgcggagctg tcatagacac ttcctcttca gaatggatag
     1261 acccaatcga gaatgcgaat gaaccatccg ggtcgctgca catcgtcgag aacaccgaga
     1321 cccgaatatt cgattctggc gtcggcgatg gcggacgaca gaaccgtatt cttggtatcg
     1381 acatcgactc ggcgcacgat gccgccgatg ctcatcaact gcttttcact gtttaccgtc
     1441 acttcgcggc gaccttcgat acgaagatcc ccgttgggaa gcacttcggt cacgatagtg
     1501 gagatggttc cggtcaaagt cccttcgcgg ctggtcgcgc cttttccgcc gaacttgttg
     1561 ttggcgcttg cgtcaatccc catccctcgt ctcgtttcat cgctgaatcg tatgcctggt
     1621 attccgatat agcccatcgc gctgccgacg agactatttt taatggtcga ctctcgctgg
     1681 gcttccgtat ccgctgattt cgatcctttg tgcttttcga cgatccttac ggtaaggata
     1741 tcgccgatgc gcatcgcgcg gagatcctca tagagatagg cccgtccgtt ttcttcctgc
     1801 catagcgatc ctacggtctt cggtggaggc aatggagaca cgacgacctt gcttgagaca
     1861 ctcggcggac ttgaacatgc ctgaaggagc agcaaggcag tgacagggaa accgactgag
     1921 gttcgtagca ggagccgact cacctgcctg aggctgaata ggtatttctt gtaggtcttt
     1981 gcccaagcca agtcctcacg cgtcttggag cgcaagttgg tgagcgtcca gtgtcctagg
     2041 agacgaggca
//
LOCUS       DASTWE010000079         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1294638, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000079 DASTWE010000000
VERSION     DASTWE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294638"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFT30_00875"
     CDS             complement(<1..409)
                     /locus_tag="VFT30_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091204.1"
                     /translation="MTFSLTLSAGDLLFLLPELFLTAWLCVILIVDFSFKRIVQEQLA
                     YLTVVGLLITLACLAWFDITGLTGALFGEMFVLDRMAIFFKVIILLATVLVILLSIDY
                     VHKFSFFRGEYYFLVAMSALGMMFMASANDLLSL"
     gene            complement(406..>1171)
                     /locus_tag="VFT30_00880"
     CDS             complement(406..>1171)
                     /locus_tag="VFT30_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5091205.1"
                     /translation="TTRDLMPIAAVLCIFSILYGGLVAYYAKDTKYVIGYSSSSHMGY
                     VFLGMAALDYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHSRDIPSLGGLSNRMPF
                     ISAAFVIGCMASIGMPGTVNFIAEVMIIVGSWNKYPFQVIVAVLGIVLTTAYLFKMMR
                     GLFYGSMAEKYSHSHDAVAVIDRLPLLIMITVSISFGIFPGHLYSVVRSGVDPLIARI
                     TKVVPLAEHPSNSPSAASPVSLRPTSPEAVAKVVAP"
BASE COUNT          338 a          295 c          337 g          201 t
ORIGIN      
        1 agagcgataa gagatcattg gctgacgcca tgaacatcat gcctaaggcc gacatggcga
       61 cgagaaaata gtactcgccg cggaagaagg aaaatttatg cacatagtcg atggaaagca
      121 gaatcaccaa taccgtcgcc aacaggatga taaccttgaa gaatatggcc atgcgatcga
      181 ggacgaacat ttcgccaaat agagcgcccg tcaaaccggt gatgtcaaac caagccaaac
      241 aggcgagagt aatgagaagc cccacgaccg tcagataggc cagctgctcc tgaacgatgc
      301 gtttgaagga aaaatcgacg atcaggatga cgcagagcca ggcggtgaga aacagctccg
      361 gcaagagaaa cagcaggtca ccagctgaca acgttaaaga aaacgtcatg gcgcaaccac
      421 cttcgcgacg gcctcaggag aagtgggccg taaggagact ggtgaggcag cagagggact
      481 gttcgacgga tgctccgcga gagggacgac tttggtaatg cgcgcgatta acggatcgac
      541 ccctgaccgc accactgagt aaaggtgtcc gggaaaaatt ccgaagctga tgctcacggt
      601 gatcatgatc aggagcggca gacgatcgat gacagccacc gcatcgtgag aatggctata
      661 tttctccgcc atggatccgt agaagagccc gcgcatcatc ttaaagagat aggccgtggt
      721 cagcacaata cccaggacgg cgacgatcac ttggaagggg tatttattcc agcttccgac
      781 gatgatcatg acttcggcga tgaaattcac agtcccgggc atgccgatgg aggccatgca
      841 accgatcacg aatgcggcgg agatgaacgg cattcgattg gagagtcctc ccagagaggg
      901 aatatcgcgg ctatgggtct ggtcgtaaac ccatccggcc atggcgaaca acatccctgt
      961 ggccatggca tgcgcgaaca tatagataac ggcgccactc aggctaatgt aatcgagcgc
     1021 ggccatgccc aagaagacgt agcccatatg gctggaactg gagtagccga tcacatactt
     1081 ggtgtctttc gcatagtacg cgaccaaacc tccgtacaag atgctgaaga tacagaggac
     1141 ggcggcaatc ggcatgaggt cgcgcgttgt c
//
LOCUS       DASTWE010000080         1492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_228403, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000080 DASTWE010000000
VERSION     DASTWE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228403"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFT30_00885"
     CDS             <1..572
                     /locus_tag="VFT30_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5091206.1"
                     /translation="MQGKLAEAEKTLTKALKLNPSGWQSHNLMGLVYDQGKRHADAIV
                     SYHTALTYRPNEPNVLNNLGLAYALSGNYESAIDAYEQAIAAGSNSPKLYNNLGIAYV
                     QRRRYFDAFNTFKKATDEPRAYNNLGVALLGVGNNKKAVVCFEKAIELNPQYYEKASE
                     NLRIARQAVPNAANGTTAASNMPEAGSCP"
     gene            complement(1024..>1492)
                     /locus_tag="VFT30_00890"
     CDS             complement(1024..>1492)
                     /locus_tag="VFT30_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091207.1"
                     /translation="LRDRREDIPELARQFLMRSAEEFGKAAVRLSPDAITKMMNYAWP
                     GNVRELQSVIHRAAAMAIGDALEGPDLDLPDHEPRELAATFVPEVISADQGGFQAMKT
                     KMIDEFERTYLSQILSTHQGNISQAARAAKKERRAFQRLLHKHGLDRRSFSAA"
BASE COUNT          335 a          448 c          365 g          344 t
ORIGIN      
        1 tcatgcaggg caagctggcg gaggctgaga agaccttgac caaagccctc aagctcaatc
       61 cttccggctg gcaatctcac aacctcatgg gtctcgtgta cgaccagggg aagcgccatg
      121 ccgacgctat cgtgtcttat cacacggcgc tcacgtatcg gccgaacgag ccgaacgtgt
      181 tgaataatct cggccttgcg tatgcgctga gcggaaatta cgaatccgcc attgacgcgt
      241 acgagcaggc catcgcagct ggttctaatt ctccaaagct ctataacaat ttaggcatcg
      301 cctatgtcca gcgccgtcgc tacttcgatg cattcaacac ctttaaaaaa gcgacggacg
      361 aaccacgcgc ctacaacaac cttggtgtcg ctctccttgg cgtcggcaac aacaagaagg
      421 ccgtcgtctg cttcgagaag gccatcgaac tcaatccgca gtactatgag aaggcatcgg
      481 agaatcttcg gattgcccgc caagcagttc ccaacgctgc taatggaacg actgctgcgt
      541 cgaatatgcc tgaggccggt tcctgcccat agaccccccg ggaccttggt agcccgattg
      601 cgcagatggc ttacttgatt gattcgaaaa gctgttgcag ttccgttctt cgggctggat
      661 tcaagccgta gatttgacgg cctgacatgc ctttccggat aagccagtct ctttcgacaa
      721 gatcattcag cgctttcctc acacccattc gatcgacagt ggctccctgc gcgcgaagcc
      781 accagttctc tattccctct gctgtatctt tcgcgtctgg atgggaacag agatacccca
      841 gtaatagcca taccactccg ttgctggttt gagactgatc cggccgttcc acgatagccg
      901 gttaccagca cgtttaatgc cgagccaaac actcttcgaa caccgcgaaa tcttgagccg
      961 tgctccgtcg aatcaactct ctgcggtaat cagtcctcag gtaacacact gccaggcatc
     1021 aatctaggcc gcagaaaaac tacgacgatc gagaccgtgc ttatgaagca accgttggaa
     1081 cgcacggcgc tcttttttcg cggcgcgtgc ggcttgggaa atattgccct gatgtgtgga
     1141 gagaatctgg ctcagatacg tccgttcgaa ttcgtcgatc atcttcgtct tcatagcctg
     1201 aaacccacct tgatcggccg atattacttc cggtacaaac gttgccgcta attcacgcgg
     1261 ctcatgatca ggcaagtcaa ggtccggacc ttcaagtgca tcgcctatcg ccatcgctgc
     1321 cgcgcggtgg atcacgcttt gtaactccct cacattgccc ggccacgcat agttcatcat
     1381 cttcgttatc gcgtctggag acagccgaac ggcggccttg ccaaactctt cggcggagcg
     1441 catcaaaaac tgtctggcga gctcggggat atcttccctt ctatcacgca gg
//
LOCUS       DASTWE010000081         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_228448, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000081 DASTWE010000000
VERSION     DASTWE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228448"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFT30_00895"
     CDS             complement(<1..204)
                     /locus_tag="VFT30_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091208.1"
                     /translation="MKEGLRDSNEMEIQKTFVPDVDADDAKASGMLGMLSRGETEESG
                     QPVMRTSRGASPFLLEALYFRSFG"
     gene            complement(466..1212)
                     /locus_tag="VFT30_00900"
     CDS             complement(466..1212)
                     /locus_tag="VFT30_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU5091209.1"
                     /translation="MSLLIPLVLLGLVLYASFKRVMEYERLVVFVLGKFQTVKGPGIR
                     LVVPVLQQMVRVNLQTVTMEVPSQDVITRDNISVKVNAVIFLRVVDPQRAVLAVQDYL
                     YSTSQVAQTTLRSVLGQSQFDDLLSKRDDINAELQRIIDQQTEPWGVKVAAVEVKNVD
                     IPQDMQRAIARQAEAERERRAKIIHAEGEFQAAQKLAEAANVISQNPAALQLRYLQTL
                     VEIAAEKNSTTIFPIPIDTLAPFMKGFLPK"
     gene            complement(1236..2555)
                     /locus_tag="VFT30_00905"
     CDS             complement(1236..2555)
                     /locus_tag="VFT30_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEU5091210.1"
                     /translation="MDDQEKKTVLDRILTAAIVGPGLALCLILPTSPALAKDIIVADY
                     EGVINPVAAEYLHDALALAQSSGALALIFKLDTPGGLDTSMRLMIKDITGSPIPVIVF
                     VSPSGGRAASAGVFITMAAHVAAMAPGTNIGAAHPVAMGGEMDNTMKEKVENDAVAYI
                     KSIAEQHGRNVSWAEDAVRKSVSATEQEALKLKIVDLIAEDIPTLLKHLQGRKIAVSS
                     GTITFSGETATLREFPMGTRLELLKILSDPNIAYLLMSIGTIGIMAELYSPGSILPGI
                     IGAISLILAFYSLQSLPVNYAGALLVILGVVFLLLEISVTSYGILALGGLAAMTLGGL
                     LLIKSDAPYLQVSLSFLLPTVMTTGALIGIIAWTAVKGGRRRPVTGAEGMIGSIGIAR
                     TDLNPRGQITLQGEIWEAISQTPIRQGEAAEVMSVEGLTVKVAPTHK"
     gene            2659..>3246
                     /locus_tag="VFT30_00910"
     CDS             2659..>3246
                     /locus_tag="VFT30_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEU5091211.1"
                     /translation="MRGMSRFSNGERIHLVDQKRRQYALTLKAGETYQFSGQKIAHDA
                     LIGRPDGSIVTLSGGKKMVALRPTFGEYVLKMPRGAQILYPKDLAIIPMWADIYPGAR
                     VFEAGTGSGALTMALLRAVGPSGLVVTYEIREDFAQTAKTNIARSLTPTNLICFRKNA
                     YESIDLPDDQVPFDRVVLDLPEPWQVVPHAMKALRS"
BASE COUNT          738 a          854 c          893 g          761 t
ORIGIN      
        1 gccgaacgac cgaaagtata aggcctctag aagaaacggg ctagcgccgc ggctcgtacg
       61 catgaccggc tggcctgatt cttcagtctc accacggctg agcattccca gcatgccact
      121 agccttggcg tcatcggcat cgacatccgg gacgaatgtt ttctgtatct ccatctcgtt
      181 ggagtctcgc agtccttctt tcatcgtgtc accttttgtt tgctctggtt gaacgtcttc
      241 tcccttatta gaggcttatt cagcggaaag gattcaaccg agtcttactt gtactgtttc
      301 tacgaagcaa aagggatgct ggattgaaga tgagaaacgc caggtatgat cagcacgaaa
      361 aatcagaaag attagactgg gctaatgagg aaatgacgga agaactgtca cacttatgcg
      421 gcatctatgt cgaatcggca tcggcgatca ggctgaaatg ccacactatt tcggcaggaa
      481 ccccttcatg aacggggcaa gggtgtcgat cggaatcggg aagatggtcg tcgagttctt
      541 ctcagctgca atctcgacga gtgtctgaag ataccgcagc tgaagagctg cggggttctg
      601 actgatgaca ttggcggctt cagcgagttt ctgcgccgct tgaaattcgc cctcagcatg
      661 aatgattttt gcccgccgtt cccgctcggc ttcagcttgc cgagcgatgg cacgttgcat
      721 gtcttgaggg atatccacgt tcttaacttc cacggcagcc actttgacac cccatggctc
      781 ggtctgctga tcgataatcc gctgcagttc ggcattgata tcatcgcgtt tcgatagcag
      841 gtcgtcgaac tggctctggc caagtacgct gcgcagggtg gtttgggcaa cttgcgacgt
      901 ggaatacaga taatcttgga ctgcaagcac ggcccgctga gggtcaacca ccctgaggaa
      961 gatgacggcg ttcactttta cggaaatatt gtcccgcgtg atgacatctt gggatggaac
     1021 ttccatcgtc acagtttgga gattgacacg taccatctgt tggaggactg gaacgaccaa
     1081 ccgaatgccg ggtcctttca ctgtctgaaa cttgcccagc acaaacacca cgagccgttc
     1141 gtattccatg acgcgcttaa agctggcata aagcaccagc cctaggagga ctaacggtat
     1201 gagcaatgac ataacggctc cttgtcttca gtattctatt tgtgggttgg ggctactttt
     1261 accgtcagcc cttcgaccga catcacctct gcagcctctc cctgccgaat tggcgtttgg
     1321 ctaattgcct cccagatttc cccctgtagg gtaatctggc cgcgtgggtt gaggtctgtc
     1381 cttgcaatcc caatcgaccc gatcattccc tcggcgcccg taaccggcct gcggcgccca
     1441 cccttgacgg ccgtccatgc aatgattccg atcaaggcgc cggtcgtcat gacagtcggc
     1501 aatagaaagg atagggacac ttgcaagtaa ggcgcatcac tcttgatgag caacagaccg
     1561 cccaacgtca tcgcagccaa gccaccaagt gccagtatcc cgtagctggt gaccgagatt
     1621 tcgagcaaga ggaagaccac gccgagtatt actaggagtg cgcctgcgta attcacgggg
     1681 agcgactgaa gcgaatagaa ggccaagatc aagctgatgg cgccgatgat gccgggcaga
     1741 atggagccgg ggctgtagag ttcggccatg attccaatcg ttccgatgga catcaggaga
     1801 taggcgatat tgggatcgct caagattttg agcaattcca gacgagttcc catcgggaat
     1861 tcgcggagcg ttgcggtctc acctgaaaac gtgatggttc cactagaaac ggcaatcttt
     1921 cttccttgta ggtgtttcaa caacgtaggg atgtcctcgg caatcaggtc gacgattttg
     1981 agttttaagg cttcttgctc ggttgcggaa acgctttttc gaactgcatc ttccgcccac
     2041 gacacgttgc gcccatgctg ttcggcgatg cttttgatgt aagcaactgc gtcgttctcc
     2101 actttttctt tcatcgtgtt atccatctca ccgcccatcg cgactgggtg tgctgctcct
     2161 atgttggtgc ccggcgccat ggcggcaaca tgggcagcca tcgtgatgaa gactcctgca
     2221 gaggcagcac ggcctcccga gggggagacg aaaacgatca ccgggatggg cgagccggta
     2281 atatccttga tcatcaagcg catggaggtg tccaatcctc caggagtatc gagtttgaag
     2341 atgagcgcca gcgcacctga tgactgagca agagcgaggg cgtcgtgaag atattctgct
     2401 gcaacggggt tgatgacacc ctcgtagtct gcgacgatga tgtctttggc gagggcggga
     2461 gaggttggga gaatcaagca gagggctaac cccggtccta caatcgccgc tgtgaggata
     2521 cggtcgagca cagttttctt ttcctgatca tccatcgagc atcatgaaaa acgtcaatgg
     2581 tgagaagatc ttacggcccc cctgcaaccc tgtcaaggaa gggcgtttgg ttgcaaacta
     2641 tggaacgaac ctcctagaat gcgcggcatg tcccgctttt ccaatggcga acgcatccat
     2701 ctagtcgatc aaaaacgacg acaatatgct ctcacgttga aagcggggga gacgtatcag
     2761 ttcagtgggc agaaaatcgc gcatgatgcg ttgatcggcc gtcccgacgg atccatcgtc
     2821 acactgtccg gcggcaagaa gatggtggcg ctccggccaa cttttggtga gtatgtactc
     2881 aagatgccac gaggggcgca gatcctgtat cccaaggatc tcgcaatcat acccatgtgg
     2941 gctgatattt acccgggtgc tcgagtgttt gaggcaggaa ccggttcagg agccctgacc
     3001 atggccctct tgcgcgcagt gggaccaagc ggcttggtgg tgacgtatga gatccgggaa
     3061 gatttcgcac agacggcaaa gacgaatatt gcccgttctc tgactccgac taatctgatc
     3121 tgtttcagga aaaatgctta tgaaagcatc gacttacccg atgatcaggt gcctttcgat
     3181 cgtgttgtgc ttgacctgcc ggaaccatgg caggtcgtac cgcatgccat gaaagccctt
     3241 cgatcc
//
LOCUS       DASTWE010000082         1398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1197754, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000082 DASTWE010000000
VERSION     DASTWE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197754"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1398)
                     /locus_tag="VFT30_00915"
     CDS             complement(<1..>1398)
                     /locus_tag="VFT30_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091212.1"
                     /translation="VVPAPQLRPWLDIGIELTEVNVVVGLEFIRQISKLAPVLPWEAV
                     RNWATVGMKLIVPNSLGKPDYMATIEFLRTSASILGNIEDPPVREKVVSLCLLLAEHS
                     PESSITWLAESPRLLEILPSMEWRVRLLQYGSLLAEKHTDVTLEYLRRASELVGLIGN
                     GPDALTRFENWFKAGMEVAAYSPNGARAYFSVESRKALASVEQALSGVPFRQVARRVK
                     LFVQGLCGTDVAVTALSDSVTSPARAMVSGDGKTISLPALLRRYPTAAENERLYLVMA
                     AHEAGHLEFGTYRLRLESLTDLVETVRGRYGRSNEARPDTLAALFQMYPNPMLVRDLW
                     TVLEDARIEFLLQTEYPGLRCDLARFAAESISPRDPAQGVTPEELIVDGLLRLSTGET
                     EDSAVPKAVREEVSILWSMCRSILKTTATAEETVRTVDQVYVRLEELLVVRGETIRAE
                     RREELANIETGQMKS"
BASE COUNT          304 a          418 c          390 g          286 t
ORIGIN      
        1 cagatttcat ctgtcccgtt tctatgtttg cgagctcctc gcgacgctct gcgcgtatcg
       61 tctcgccgcg aactaccagc agttcttcca gccgaacata gacctgatcg accgtgcgaa
      121 ctgtttcttc tgccgtggcc gtagtcttga gaatggaacg gcacatgctc cagaggatag
      181 agacttcttc cctgacggcc ttgggcaccg ccgaatcttc cgtttcaccg gttgagagtc
      241 tcaggagacc atcgacgatc agttcctcag gcgtcactcc ttgagccggg tcgcgcggag
      301 agatggattc agcagcgaat cgtgcaaggt cgcaccggag tcccggatac tcggtttgca
      361 gcaaaaattc gatacgcgca tcttccagca cggtccagag gtcccgcacc agcatggggt
      421 ttggatacat ctgaaaaagg gcggccagtg tgtccggtct agcttcattg gatcgaccat
      481 accgacctcg tacggtttcg accaaatcgg tcagcgactc aagccggagg cgataggtgc
      541 cgaactccaa atgcccggct tcatgcgccg ccatcacgag atagagacgc tcgttttccg
      601 cggcggttgg ataccgacgg agcagcgccg gcaacgaaat ggtttttcca tcgccgctga
      661 ccatcgcacg agccggggac gtcacagagt ccgagagcgc cgtcacggcg acgtcggttc
      721 cacagagtcc ctgaacgaac agtttcactc gtcgcgcgac ctgtcgaaac ggcaccccgc
      781 tcagggcttg ttcgaccgaa gccagtgcct ttcgcgactc cacggaaaag taagcacgcg
      841 ctccgtttgg actgtaggcc gctacttcca tcccagcctt aaaccaattt tcaaatcgcg
      901 tgagcgcgtc gggtccattt ccgataagtc cgacgagttc ggaagcacgt cgcaggtact
      961 caagtgtcac gtcggtgtgc ttctcagcaa gcaaggagcc gtactgcaac agccggaccc
     1021 tccattccat ggacggcaat atttccaaga ggcgggggga ctctgcgagc cacgttatgc
     1081 tggactcggg agaatgttca gccaacagga ggcacaggga tacgaccttt tcccgaacgg
     1141 gagggtcttc gatatttcca aggatcgacg cgctggtcct gagaaactca atggtcgcca
     1201 tatagtccgg cttcccgagg ctattcggca cgatcaattt catgcccacc gtggcccagt
     1261 tgcgcaccgc ctcccaagga agcacggggg ccagcttgga aatttgtcgg ataaactcaa
     1321 gcccgacgac gacattgacc tcggtcagtt caatgccgat atcgagccag ggtcgcaatt
     1381 gcggagcagg aacaacgg
//
LOCUS       DASTWE010000083         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_2284, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000083 DASTWE010000000
VERSION     DASTWE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..369)
                     /locus_tag="VFT30_00920"
     CDS             complement(49..369)
                     /locus_tag="VFT30_00920"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HEU5091213.1"
                     /translation="MVRSHHEQPYRARALKLFPWICAHCGREFDGKKLSQLTVHHKDH
                     NHHNNPPDGSNWELLCLYCHDNEHQRDQVGGGSNESQSRPETDRPFAPFANLANLLKR
                     NTAA"
     gene            580..1317
                     /locus_tag="VFT30_00925"
     CDS             580..1317
                     /locus_tag="VFT30_00925"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEU5091214.1"
                     /translation="MKEKKEKGRGRDADKTSEIRVGSDPFQRLLGLMFKAHPWHGVSI
                     GEEAPDVVTAYIEIVPTDTVKYEVDKSSGFLKVDRPQRFSNYCPVYYGLIPQTYSGEQ
                     VARIFAKRARRKDMVGDGDPLDICVLSEKSIPHSDILLTALPIGGLSLADGGEADDKI
                     IAVMRDDAVYGNFTDISQCPTSLMDRLQHYFLTYKSAPGTTHHRVEITGVYGREEALK
                     VIRASHADYRAKYPELSSLWPKNLSLV"
     gene            1448..1768
                     /locus_tag="VFT30_00930"
     CDS             1448..1768
                     /locus_tag="VFT30_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091215.1"
                     /translation="MNRRMLIMVWTLALVFGLNYAGMVTMVSADESTVDLNSPDAIKS
                     ALEQQVGKRVKVKLESGHDLEGKVAKVGSQAVQLAELTGMEFFDATVKVDEIAAVIVR
                     GRTK"
     gene            complement(1818..2186)
                     /locus_tag="VFT30_00935"
     CDS             complement(1818..2186)
                     /locus_tag="VFT30_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5091216.1"
                     /translation="MVHFSHTRTYQRFPFHAPLIIGGESFVNEGTLRNLSMHGCSMAC
                     DRELPLGSTVRVSLLLPDQQSALPIESGRITWVQGNECGIEFVQLPLQSRMRLNRTLR
                     VELIQFLNARKNRELETRTA"
     gene            2701..>3100
                     /locus_tag="VFT30_00940"
     CDS             2701..>3100
                     /locus_tag="VFT30_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5091217.1"
                     /translation="MRVEILFCGICHSDLHQVRNEWSGAMPTVYPCVPGHEIVGRVTD
                     VGAAVTAFKPGDLVAVGCMVDSDGTCSECRDRLEQFCPNVVFTYNSRDKHLGGITYGG
                     YADGIVVDERFVLRVPPNLDLAGAAPLLCAG"
BASE COUNT          703 a          801 c          914 g          682 t
ORIGIN      
        1 tgacggtagc gagaaacaag gcgattccat atttcgggca tcgcctgatc aagctgcagt
       61 gttgcgttta agcaggttgg cgagattggc gaagggtgcg aacggtcgat ccgtctccgg
      121 cctcgactga gattcgttcg atccaccacc gacttgatca cgctgatgtt cgttgtcgtg
      181 acaataaaga cagagtaact cccaattgct gccgtcagga ggattgttgt gatggttgtg
      241 gtccttgtga tgaaccgtga gttggctcag ctttttgccg tcaaactcgc gtccgcagtg
      301 agcacaaatc catggaaata gtttcaatgc gcgcgctcgg tagggctgct cgtgatggct
      361 ccgaaccatg cgagcctcct gagaagggtg actcggcaca gagatattgt acgccaagtc
      421 tgcaaaatga accatacggc gaaaacggtt cctaccatct cctcttcgcc ctgatggggg
      481 gaaggggata ggtggtgaga cactattgcc cctctcacgc tcctgggaga tactggggcc
      541 gcggtcgcca ggaatggtct tcagataagc ggaggggaca tgaaagagaa aaaggagaag
      601 ggaaggggaa gggacgcaga caagacgagt gagatacgcg taggaagcga tccctttcaa
      661 cgtctgctcg ggctgatgtt caaggcgcat ccatggcacg gtgtgtccat cggggaggaa
      721 gccccggatg tggtcacggc ctatattgaa atcgtcccga ccgacacggt caaatatgag
      781 gtggacaaga gcagcggctt cctcaaggtg gacaggccgc agcggttttc caattactgt
      841 ccggtctatt atggactcat tccgcagacc tactctggtg agcaggtggc gcgtatcttt
      901 gcgaagcgag cccgccgcaa ggatatggtc ggggacggcg atccgctcga tatctgcgtg
      961 ttgtcggaaa agagcattcc gcacagcgat atcctcctga ccgccctgcc gatcggcggg
     1021 ttgagcctgg cggacggcgg tgaggcggac gacaagatca tcgccgtgat gcgggacgat
     1081 gcggtgtacg gcaacttcac ggacatttca caatgcccaa cgagtttgat ggatcgcctc
     1141 caacattatt tcctcacgta taagagcgca cctgggacga cgcatcatcg ggtggaaatc
     1201 accggtgtgt atggacgaga ggaagcgcta aaggtcatcc gcgccagcca cgccgactat
     1261 cgcgcgaagt atcctgaact gagttccttg tggccaaaga atttgtcgct tgtatgaatg
     1321 atcccactga cgttgccagt tggcattttc accggaacag ttcaccgaga tactgtgtaa
     1381 ctgtttggaa cgggggaggc agatgacatt cacgatattg aaaccgctca ctcacaagga
     1441 ggatttcatg aatcgacgga tgctgatcat ggtctggacc ctggcactgg tcttcggtct
     1501 taattatgcg gggatggtaa caatggtgtc ggcggacgaa tcaaccgtgg acctgaacag
     1561 cccagatgcc atcaagtcag cgctcgaaca acaggttgga aaacgggtga aggtcaagct
     1621 ggagtcgggt catgacctgg aaggcaaggt cgccaaggtc ggttctcagg ccgttcagct
     1681 ggcggagctg accggaatgg agttcttcga cgccacagtg aaggtggatg aaattgccgc
     1741 agtgatcgtg aggggccgca cgaagtaatc ggacacgcgg attttcagcg tatgattctg
     1801 ataccgttgc ggggatccta agccgtgcgt gtctctaact cccgattctt gcgtgcattc
     1861 aaaaattgaa tcagttcgac tcgcagcgtg cgattgagac gcatccgcga ctgcagcggc
     1921 agctgcacaa attcaatgcc gcattcattt ccctgcaccc aagtaatccg gcccgattca
     1981 atcggcaatg cagactgttg atccggcaag agcagactta ctctcaccgt gctgccgagg
     2041 ggtagttcgc gatcgcaggc catcgaacaa ccgtgcattg agaggttccg aagcgtgccc
     2101 tcattgacga acgattctcc gccgataatg agcggagcgt ggaagggaaa ccgttggtag
     2161 gttcgcgtat gagaaaagtg caccatcgtc gcctccctct gatggcggga ttgtatctgg
     2221 ccatgactcg gcacgatatc ctacattggg aggccactgg tatggatgca gttgtagtta
     2281 tgcgaattta agagggcatt gcgaagcgaa ggcaggttca tcgcattcac ccccatccct
     2341 gagtcatgat cgggcttgcc aacgacagga tcgccgcgca ccgaccctga agtgcagttt
     2401 gccccggcct tttctcttta tttctattcc taggggcact accagatacc ataaatcgaa
     2461 ccctgtatta ctgtgcttcc ggagtataag catacttgct attgcttatc cgaccacccc
     2521 aaaggcatgc ggttaggcga gcggaacaga cttatctaga gttatgggtt tgactggcct
     2581 tcagggaagg aagaatgcaa catggcaaag acatggaata gcaaagcata tgctgctgca
     2641 agtgctctat catcgctggc ccctatcact atcccacgac gcgagccaat ggaacgcgac
     2701 gtgagagtgg agattctctt ctgtggcatc tgccattccg acctccatca ggtccggaat
     2761 gagtggagcg gtgccatgcc cacggtctac ccttgcgttc cgggccacga gattgtcggg
     2821 cgcgtcaccg atgtcggcgc cgccgtcact gcgtttaagc ccggggatct tgtagcggtc
     2881 ggctgcatgg ttgattccga cgggacctgc tcggagtgtc gggacaggct cgaacagttc
     2941 tgcccaaacg tggtctttac gtataactct cgggacaaac acctcggagg aatcacctac
     3001 ggtggatatg ccgacggcat tgtcgtggac gagcggttcg tcctacgcgt tcctcccaat
     3061 ctcgatctcg ccggcgccgc gccgctgctg tgcgctggaa
//
LOCUS       DASTWE010000084         1036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1133218, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000084 DASTWE010000000
VERSION     DASTWE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133218"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1036)
                     /locus_tag="VFT30_00945"
     CDS             complement(<1..>1036)
                     /locus_tag="VFT30_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091218.1"
                     /translation="VSPHVMFSEADLAQVRSRLAKERLRFEEQLEQSIVERQQQSQTV
                     QQAEQRLDLLRNKRTVGKDSVEETPAISRGKAAVELARARMDNLVSQSDLLQQLMNVV
                     EGERQLWENRFVIVHTSEPGKAREAYERFTPLFDNFRASRDYLRQQTGIISGQISEVE
                     NRMRHSSAPLRAMLQDLIQTFHEREEAYTRALHRMDEAARFMERWRDEFKQQQKEVPL
                     SDRLEELLARMGSLVKDAWRYELFSAEDTIEVDGKTMTGHRSVTVGKTLTALAIFLIG
                     YVVCVHLARAIGRLAVTRLSMGSDVANLLRQWSQALLITFLIVLSFSWAKIPLTIFAF
                     LGGAFAIGVGF"
BASE COUNT          224 a          317 c          262 g          233 t
ORIGIN      
        1 gaatcccaca ccgatcgcaa aggccccgcc gagaaacgca aaaatcgtca atggaatctt
       61 agcccaagag aagctcagca cgatcaagaa cgtgatgagc agcgcttgac tccactggcg
      121 cagcagattc gcgacgtccg accccatgct gagcctcgtc acggccaatc ggccgatggc
      181 tcgcgccaag tgcacacaga cgacatagcc gatcagaaat atcgccaagg cagtcagcgt
      241 tttgccgact gtgacactgc ggtggccggt cattgtcttg ccgtcgacct cgatcgtatc
      301 ttccgcggaa aagagctcgt atctccaggc atccttgacc aggctcccca tacgggcgag
      361 caattcttcg aggcgatcgg atagcggcac ttccttctgt tgttgcttga attcatcccg
      421 ccatctctcc ataaacctgg ccgcttcatc cattcggtgc aatgcccgcg tgtaggcctc
      481 ttctcgctcg tgaaatgtct gaatgaggtc ttgcagcatg gcacgaagtg gcgccgacga
      541 atggcgcata cgattctcaa cttcgctaat ctggccggat ataatcccgg tctgctggcg
      601 caaataatcc cgggacgcac gaaaattgtc aaaaagcggc gtgaatcgtt cataggcttc
      661 ccgcgctttc cctggctcgg acgtatgaac gatgacaaag cggttttccc acagttgacg
      721 ctcgccttcc acgacattca tcaattgctg caagagatcg ctctgcgaca ctagattgtc
      781 catacgggct cgtgccagct caaccgccgc cttcccccgg gatatggccg gcgtctcctc
      841 taccgaatcc tttcctactg tccgcttgtt tcggagaagg tctaatcgct gctcggcctg
      901 ctgtacggtc tgggactgct gttgtcgctc aacgatcgac tgttccagtt gctcctcgaa
      961 acgcaagcgt tctttcgcca aacggcttcg gacttgagcg aggtctgctt cgctgaacat
     1021 gacatgagga gaaacc
//
LOCUS       DASTWE010000085         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_907157, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000085 DASTWE010000000
VERSION     DASTWE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907157"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFT30_00950"
     CDS             <1..468
                     /locus_tag="VFT30_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091219.1"
                     /translation="YSEYIEDVADWWREKVGKDWLAMRNRAMELLSEENRLSQIVKLV
                     GPDALPYAERMILETSRLLREGILQQNATDPVDAFSPIEKQIRMLELVLYFHERGMSI
                     IRRGAPINLIHDLPVVDQLIRMKSNVSNDDLSKFDDIQKAIDEQMSQLDAEYR"
     gene            465..>1041
                     /locus_tag="VFT30_00955"
     CDS             465..>1041
                     /locus_tag="VFT30_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091220.1"
                     /translation="MNEATVLGGQEVVGVGRIEGPILIVEGATNVSYDEVVEIRDSRG
                     QLRRGRVLEVGEGTAVIQVFAGSTGLSVDGTRIRFLGNTLHMPVAEEMLGRVFDGLGN
                     PIDGGPQPLTDRYADVNGQPINPTARIYPRDYIQTGISTIDGLNTLLRGQKLPIFSGA
                     GMPHDQLAAQIVRQATLGKQAGAAAESEAEQF"
BASE COUNT          237 a          268 c          314 g          222 t
ORIGIN      
        1 tacagcgaat atattgaaga tgtagcggat tggtggcgcg agaaggtcgg caaggactgg
       61 cttgccatgc gtaaccgggc catggaattg ttaagtgagg aaaaccgctt atcgcagatc
      121 gtcaagcttg tgggtcccga tgccctgccg tatgcggaac gcatgatcct tgaaacgtcc
      181 cgcctcctgc gcgaagggat attgcagcag aacgccacag atcctgtgga tgccttttcg
      241 ccgatcgaga agcaaatccg catgctggag ctcgtcctgt acttccatga gcgcggcatg
      301 agcatcatcc ggcgtggtgc tcccattaac ttgatccacg atttgcctgt ggtagatcag
      361 ttgatccgta tgaagtccaa tgtttcaaat gatgatttga gtaaattcga cgatattcaa
      421 aaagcaattg acgaacaaat gagtcaatta gatgcggagt acagatgaac gaagcaaccg
      481 tacttggcgg gcaggaagtc gtcggtgttg gccgaattga agggccaatt ttgatcgtgg
      541 aaggtgccac aaacgtcagc tacgatgaag tggtggaaat ccgtgattcg agaggccaac
      601 tccggcgcgg gcgcgtgctg gaagtgggcg aggggacggc ggttatccag gtcttcgccg
      661 gttcgactgg cttgtctgtg gacggtaccc ggatccgttt tctggggaat acgctccaca
      721 tgccggttgc cgaagaaatg ctgggtcgcg ttttcgatgg tctgggcaac ccgatcgacg
      781 gcggcccgca gccgctcaca gaccgttacg cggatgtgaa cggccagccg atcaacccca
      841 ccgcccgtat ctatccacgc gattacattc aaacaggtat ttcgaccatc gatgggctga
      901 acacgctcct gcgcgggcag aagctgccca tcttctccgg tgccggtatg ccccatgatc
      961 agctggcggc gcaaatcgtc cgtcaggcta ccctggggaa acaggctgga gccgcggcag
     1021 agagcgaagc cgagcagttc t
//
LOCUS       DASTWE010000086         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_907201, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000086 DASTWE010000000
VERSION     DASTWE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907201"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFT30_00960"
     CDS             complement(<1..929)
                     /locus_tag="VFT30_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5091221.1"
                     /translation="MDSSENSPDPVSLIQTEDRYRDYFENAKDAIYVHDLNGRYIMVN
                     KAGEELIGYSREELLQMRISDVVPRNYLDEIHTRLKEKLDDHSLTIYEVEAIRKDGTR
                     VPIEVSSRLVYENGVPVAVQGSARDITERKRAEEALRASQFQLQQSQKLEAIGQLAGG
                     VAHDFNNMLTAIIGYTDLSLRRVGLENPIRRNLEETKKAAERAAALVRQLLAFSRKQI
                     LEPKVLDLNDVVKDMEKMLTRLIGEDIKLTTRLEPDLGSVKADPSQVEQIIVNLVVNA
                     RDAMPRGGRMTIETANTRLDDQIGLRHVSVKPG"
BASE COUNT          230 a          303 c          307 g          296 t
ORIGIN      
        1 tcacccggct tgacggaaac gtgtctcaga ccaatttgat catcaagcct cgtgttggct
       61 gtttcaatgg tcattctgcc gccgcgaggc atcgcgtcgc gtgcattgac gacgaggttc
      121 acgattatct gttcgacctg agatggatca gccttgacgc ttcccagatc gggttcgagc
      181 cgcgtggtaa gtttgatgtc ttcaccgatc agacgcgtca gcatcttctc catgtccttt
      241 actacgtcat ttaaatcaag caccttcggc tcgaggattt gtttgcgact gaaagccagt
      301 agttggcgca ctaaagcagc cgctcgttcg gccgctttct tcgtctcttc gaggttccgg
      361 cggatcgggt tctccagtcc gactcgtctg agcgacagat cggtgtagcc gataatggct
      421 gtcagcatgt tgttgaagtc gtgagctaca ccgccggcta gttgtccgat ggcctcgagt
      481 ttttgcgact gttggagttg aaattgactg gcacgaagtg cctcttcggc acgcttgcgt
      541 tccgtgatat ccctggcgct gccttgcacg gccacgggca caccgttctc atagaccagg
      601 cggctgctca cttctatcgg aacacgagtg ccgtcctttc ggatcgcttc gacttcatag
      661 attgtcaggg aatgatcgtc gagcttttcc ttcagccgcg tgtgaatctc atcgagatag
      721 ttgcgtggaa cgacatcgga gatcctcatt tgaagtagtt cttcacgcga atagccgatc
      781 agctcttcgc ccgccttgtt aaccatgatg taacggccat tgagatcgtg gacgtaaatc
      841 gcatctttcg cgttttcgaa atagtcgcga taacgatctt cggtttggat aaggcttacc
      901 ggatcaggtg agttttcgga agaatccata actgcattta aagcgaacaa gccgagagcg
      961 cggcgcgtcc atcagttgcc aatggaccgc cacgccctca acgcacacgc ggcctcctcg
     1021 actagacctg ccccggtgcg ttaccgctga tcggtgtctc tccccaagtc cgatcaacga
     1081 ttagaatgga ggaatatagg ggaagggatt ggtgtgtagt atcgggcgag tgatga
//
LOCUS       DASTWE010000087         1160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1327232, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000087 DASTWE010000000
VERSION     DASTWE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327232"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFT30_00965"
     CDS             <1..559
                     /locus_tag="VFT30_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091222.1"
                     /translation="AEQQRLDDVMTARLSRKSEGGNKEQTELVPFEFQGLEAVLSKDP
                     ELAISKGLAWFAQDREEFRYRGGSLLGRAFPTCTPEFAAALGELVKTGGDTEADFALA
                     ILQNYLGSTSTDGILKEIVSRFPHDARKMDGVRISINSTGVVSGEFGLAEAWRVKKES
                     LTRWLTDERQTVKAFAELHMQNSTE"
     gene            complement(731..1048)
                     /locus_tag="VFT30_00970"
     CDS             complement(731..1048)
                     /locus_tag="VFT30_00970"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5091223.1"
                     /translation="MSEKITRGSGNVFRDLGFENPEEYQVKADLALHIIKIIEDRSLT
                     QKEAAAVIGATQPDISKLKNGQLTGFTLDRLFSFLLRLNRNIEIRVTKTRKRSGKLAT
                     VAA"
BASE COUNT          272 a          309 c          335 g          244 t
ORIGIN      
        1 ggctgaacag cagcgactcg acgacgtgat gactgctcgg ctctcaagaa agtctgaggg
       61 cggcaataaa gaacagaccg agctggtgcc gtttgagttt caagggctgg aagcggtgct
      121 gtcgaaagat cctgaactcg ccatcagcaa gggactggcg tggttcgctc aagaccggga
      181 ggaattccgc tatcgaggag gtagcctact cggcagagcg tttcctacat gcacgcccga
      241 gtttgctgcc gcgctcggcg agttagtcaa aaccggcggc gacacggagg cagatttcgc
      301 cctggcgatt ctccagaact atcttggctc gacatccacc gacggcatct tgaaagagat
      361 tgtgtcgcga tttccgcatg acgctcgaaa aatggacgga gtccggatca gtattaacag
      421 caccggcgtg gtctccggtg aattcgggct cgcggaagca tggcgggtca agaaggaatc
      481 gctgacccga tggctgaccg acgaacgcca gacggtcaaa gcattcgccg aactgcacat
      541 gcagaactcg accgaatgat cgtgacagag cgacgccgtg tggaggccga acgggaacgg
      601 cgagagagga gccggcacga ggaagaatct ggccacgatc atggttacag gccgaaaccc
      661 ttctgatagc agaaaaagat atggtcaggt gagctagccc gggtttgaat gccgaacaac
      721 gcccggccga tcaagcggcg acggtggcca gcttgccgga tctctttctc gtcttggtca
      781 cgcggatttc aatattcctg tttagccgaa gcagaaacga gaataatcga tccagcgtga
      841 agcccgttaa ctggccgttc ttgagcttgg agatatcagg ctgtgtcgca ccaatcaccg
      901 cagcggcttc cttttgtgtc aaggatcgat cctcaatgat cttgataatg tgtagggcga
      961 gatccgcctt gacctgatac tcctcgggat tctcaaaccc aagatcccga aaaacgttgc
     1021 ctgaccctcg tgtaattttc tcgctcatgg ggtggctcct ttactgtctg cgattgctag
     1081 cttcaaacgc tgcgcgatca gttccatctc cttcgacggc gtggcaatac cgcgcttcga
     1141 tttcttctgg aaacaatgca
//
LOCUS       DASTWE010000088         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1262682, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000088 DASTWE010000000
VERSION     DASTWE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262682"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1201)
                     /locus_tag="VFT30_00975"
     CDS             complement(263..1201)
                     /locus_tag="VFT30_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5091224.1"
                     /translation="MTSLASYAHLRSRLQLALAINAVIIAAEFVGGWLLNSIGLMSDA
                     GHNLVDQGSLFLALYAHLLTARPASESRTFGYHRAGIIAAFLNSFILLLTALGITLVG
                     VKRLLQPVPIDGGWVMVVAAVSFVANLGIALLLQHGAKDDLNIRSAFWHMVGDAWVSL
                     GVIVSGGAIFLTGWTVLDPLVSLLVVGAIVRGAWPIFKESMEVLLESTPPGISASHVA
                     ATMEAIPGVRNVHDLHIWAVEPRLVMLTSHVMIEHHRTPLELLQLIQNRITTDFGIKH
                     MTIQLETECCDPDDIHCDLRKLTGHHHEPQTFAHHH"
     gene            complement(1276..>1761)
                     /locus_tag="VFT30_00980"
     CDS             complement(1276..>1761)
                     /locus_tag="VFT30_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091225.1"
                     /translation="ISAEGPLAYPAALQRTSQTLLLIPGQDLTNDGIVLHLHGYLVQI
                     ERPQPREALIAKDDVKLRASVRTLSGSLVRPHGDWDSRKIRIYGEVLIGDRIIERLQL
                     FYDEGSRAFEAPFFVPPSKEVPDGIKLRVIATDLSTGNSGMDQANYPVFSERLPPKSG
                     R"
BASE COUNT          395 a          488 c          489 g          389 t
ORIGIN      
        1 ttccatcaca tgctagtgtc tcccatttca taggtatatc cctggtgaga gttgccactg
       61 aactttcaca acaccctagc tgttctccac ccgactctag ttctaatcgc tgcttaattg
      121 catttgacct gcttaagtaa ctccgagaat ccctgattta attgtcaggt atgcgagcaa
      181 gcaatgcggc ccaaatttca atttttagac ttcgataaac ctcaggagca taaagtctac
      241 accttccctt ccttccctat gatcaatgat gatgcgcgaa ggtctgtggt tcgtggtggt
      301 gtccggtcag cttccgaagg tcgcagtgga tgtcgtcagg atcgcagcac tcggtttcca
      361 attgaatggt catgtgtttg atgccgaaat ccgtcgtgat ccggttctgg atcagctgca
      421 gcaactccag cggggtgcga tgatgttcga tcatgacgtg gctggtgagc ataaccaacc
      481 gaggttcgac cgcccaaatg tgcagatcat gcacgttcct aacgccgggg atggcttcca
      541 tcgtggctgc gacatgagac gcgctgatgc ccggcggtgt cgattccagt aagacctcca
      601 tcgattcttt gaagatcggc caggctcctc gaacgatcgc gcccaccacc agcaagctca
      661 cgagcggatc caggaccgtc cagcctgtca ggaaaatagc cccgccgctg acaatgacgc
      721 cgagcgagac ccaagcgtct cctaccatgt gccagaaggc gctccgaatg tttaggtcgt
      781 ctttcgctcc gtgctggagc aacagggcaa tcccgagatt ggccacgaaa ctgacggccg
      841 cgacaaccat cacccatcca ccgtcgatgg gaaccggttg caggagtcgc ttcacgccga
      901 cgagtgtgat gccgagtgcg gtcaacagga ggataaaact gttgagaaaa gccgcgatga
      961 tgccggcgcg atggtagcca aaggtcctgc tctctgaagc tgggcgcgct gtgaggagat
     1021 gagcatacag tgccagaaag agcgaccctt gatcgacgag attgtggcct gcgtcgctca
     1081 tgagtccaat actattgagc agccagccgc cgacgaactc agccgcgatg atcacggcat
     1141 tgatggctaa ggccagctgg agtctggatc ggagatgtgc atacgaagcc agtgacgtca
     1201 tgcatgacag tttcgcacgg aggacgcgac gcagcaagtc ttgccgaagg tttccatcaa
     1261 agaagttgca gccgactacc ggccggattt gggtgggaga cgctcgctga acaccggata
     1321 gttggcttga tccattcctg aattgcccgt agacaggtcc gtcgcaatca ctcggagctt
     1381 gatcccgtcg ggaacttcct tcgacggcgg cacaaagaac ggtgcctcaa aagcgcggct
     1441 cccttcgtca taaaacagtt gtagtcgctc gatgattcgg tccccgatca acacctctcc
     1501 ataaatacgg atcttccgcg agtcccaatc tccatggggg cggaccaatg agccggacag
     1561 ggttcggact gaggctcgga gtttcacgtc gtcctttgcg atcaatgcct cacgtggctg
     1621 gggccgctcg atctgaacca ggtagccatg gagatgcagg acaatgccgt cgttcgtcag
     1681 gtcttgtccc ggaatcaaca acaaggtttg gctggtcctc tgtaatgcgg cgggataggc
     1741 gagtggtccc tctgctgaga t
//
LOCUS       DASTWE010000089         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_131665, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000089 DASTWE010000000
VERSION     DASTWE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131665"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFT30_00985"
     CDS             complement(<1..576)
                     /locus_tag="VFT30_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5091226.1"
                     /translation="MLQLESVHKQFATKVLLEGATAHLRPGARVGLVGPNGVGKTTLF
                     RMILGEESPDKGSIRKRPRLRIGYLPQELETIVGKTVLDAAHRDLYPEHEAKRILSGL
                     GFSEADFARPIENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFERFLLD
                     SNLTLLVISHDTKFLDGIATHIWELRDRTIQE"
     gene            complement(721..796)
                     /locus_tag="VFT30_00990"
     tRNA            complement(721..796)
                     /locus_tag="VFT30_00990"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Ala,seq:ggc)
BASE COUNT          260 a          280 c          311 g          213 t
ORIGIN      
        1 ttcctgaatc gtgcgatcgc ggagttccca gatgtgggtg gcgatgccgt ctaaaaattt
       61 tgtgtcatgg ctgatgacca acagtgtgag attggaatcg aggagaaatc gttcgaacca
      121 acgttgcgtg ggcttgtcca aatggttcgt tggctcatcc agcatgagga catcgggatt
      181 tgacaataac aaatgcgcca atgccactcg catgcgatag ccgccagaaa gattctcaat
      241 cggtcgggcg aaatccgctt cagagaaccc taagccggaa agaatgcgtt tggcctcatg
      301 ttccggatag aggtctcggt gcgcggcatc caagacggtc ttcccaacta tggtttccaa
      361 ttcttgtggg aggtagccga tgcgcagccg cggacgctta cgaatggagc ctttatcggg
      421 agactcctct ccaaggatca tccgaaacag cgtcgtcttg ccgacgccgt taggccccac
      481 gagacccacg cgggcgccgg ggcgcaaatg cgcggtcgct ccctcgagga gcaccttggt
      541 cgcaaactgc ttatggacgg actcaagttg cagcatcgat cattgagtgg tttggtcgta
      601 caggtgaagc gttcggcgag aatccgccac cgcctcgaca caacagaggc gaagcagtca
      661 agacagttga ctaatcggta accccgcaca cattcagact cgttatcgag caagcgttat
      721 tggtggagct ggccgggatt gaaccggcga cctcgtgaat gccattcacg cgcgctccca
      781 actgcgccac agccccacgc ttgtatacat gtgcacaggc acggaataga aaatcactgc
      841 agagacaagt gtgcaactta acacggcctg tttttgtcag tcaagaaaga gcgagagagg
      901 cgcaatccct cgacagtgct cgctggacgc gcgcaggaaa ggatcgaggg gctacctcgt
      961 gagcaaagcc gcagaagacg gtcattggac ggccacggta gccgggtatt ggaacatcgc
     1021 caactgagaa ggtgaactgg ttccgaaaga tcggagtagc atgt
//
LOCUS       DASTWE010000090         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_842614, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000090 DASTWE010000000
VERSION     DASTWE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842614"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFT30_00995"
     CDS             <1..981
                     /locus_tag="VFT30_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5091227.1"
                     /translation="TQTVSLVREAVEAVTAGAEEISKGSDDLAQRTGQQAGALEETSA
                     SMEEMTSTVKQNADNSKQANQLAIAARDIANKGGTVTTRAVEAMGEINKSSKQIADII
                     TVIDEIAFQTNLLALNAAVEAARAGEHGRGFAVVAAEVRNLAQRSATAAKEIKGLINE
                     SIQRVTDGTELVNQSGKTLEEIVGSVKRVTDIIAEISAASQEQASGIDQVNKAIMQMD
                     EGTQQNAALVEETASASQSMKEQAKELMRQVEVFKIQQSESRRAAEAAKRESAVHGKQ
                     PAKPAVSANNPKNTKRPSISGRSVEEYQPVGLTAGNGKDSRNKADEFEEF"
BASE COUNT          301 a          270 c          352 g          169 t
ORIGIN      
        1 actcaaacgg tgtcactcgt gcgcgaagca gtcgaagccg ttacagccgg ggcagaagaa
       61 atcagcaaag gcagcgatga tctcgcgcaa cggaccggtc agcaggcggg ggccttggag
      121 gaaacctccg cgtcaatgga agaaatgacc tctaccgtca aacagaacgc cgacaattcc
      181 aagcaagcga atcagttggc cattgccgca cgcgatattg ccaataaggg tggaacggtc
      241 acgacaaggg cggtcgaggc aatgggagag atcaataaga gcagcaagca aatcgccgac
      301 atcatcacgg tcatcgacga aatcgccttc cagactaatc ttctggcgtt gaatgcggcg
      361 gtggaagcgg ctcgggccgg ggaacatggg cgaggctttg ccgtggtggc ggctgaggtg
      421 cggaatctag cccagcgttc cgcaaccgcc gcgaaagaaa tcaagggatt gatcaacgag
      481 tcaatccaac gcgtcacgga cgggaccgag ttggtcaatc agtccggaaa gacgcttgag
      541 gagatcgtcg ggtccgtcaa acgcgtcact gatatcatcg ctgagatcag cgctgcatcg
      601 caggagcaag ccagcggtat cgatcaggtg aacaaggcca ttatgcagat ggatgagggt
      661 acccagcaga atgccgcttt ggtcgaggaa acagcctctg ccagccagtc gatgaaggaa
      721 caggcgaaag agctgatgcg acaagtggag gtgttcaaga ttcagcagtc tgaaagtcgg
      781 cgagcggcag aggcggcgaa gcgggagagt gccgtacacg gcaaacagcc ggctaaacct
      841 gcggtgagcg cgaacaatcc gaagaacacg aagcggccgt cgatctccgg cagatcagtc
      901 gaggagtatc aaccagtcgg tctcacggca ggcaacggaa aggacagccg caataaggcg
      961 gacgagttcg aggaatttta aacgcttgcc tgaccgaagc gtccagacgg accgtggagt
     1021 ggattgctaa agagatcaag ctatgatgga ggatgaggtg ccgatgacac ccggcaaacg
     1081 tgtttggaag ga
//
LOCUS       DASTWE010000091         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1165723, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000091 DASTWE010000000
VERSION     DASTWE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165723"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFT30_01000"
     CDS             complement(<1..741)
                     /locus_tag="VFT30_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091228.1"
                     /translation="MDKPRHFLPQTIVTALLLFQAGCGATQLGMCAPHTKEEGMPTRN
                     WAECFDQPFSHEGITHSVFCLHDGTDKPPVLLLHELTGLSPGTLAYAEELSKDFTVYV
                     PLLFGEKGKFSLTKGLWAYWFRGLMDFFPSGEWGVPSQGSAPVVNWLRGVVQKVGERH
                     AQQHIGIIGNCLTGPIPLALLNHPRVSATVVAQPALPLRFWWYTDADKKSLGLTADDL
                     QYARKSAVKIYGLRFETDCMSDPAKQETL"
     gene            complement(1155..2804)
                     /locus_tag="VFT30_01005"
     CDS             complement(1155..2804)
                     /locus_tag="VFT30_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5091229.1"
                     /translation="MSRTAAFRSFAQMMTAALLADGRHCPATEALGVTQESASYPTHD
                     VLSRRDFLLRAGMTAAALAISTIPGFPHRATAAKPLPSSLSVGIVGAGLAGLACADTL
                     KTRGIRASVYEAATRAGGRCWSLRGFFPGQVAERGGELIDNLHKTMLGYAKRFHLSLE
                     DVNKEPGEIFYYFSGQGYSEAAVVAEFRDFVSIMRTDLNRLSREVTATSHTPDDIALD
                     RTNLLTYLEGHNGAGIPAGPLAKAAIIAAYEAEYGLAAGEQSCLTFLLFIHADRRSKF
                     TPFGVFSDERYHLVDGNDRIIEGLTRELPGQITYDSRLVRVRRLSDGRIELTFQQGSR
                     TMTFTHDVAVLTIPFTTLRHVDLDAGLGLRPAQRATIQGLGYGTNAKMMIGFSSRPWR
                     ALGGNGTAYVTDLAHAQTTWETNPTLGSETKGILTDYSSGPRGTRLDPDAVQDEAQRF
                     LQDLNLIYPGALGAATLVQGQHLAHLEHWPSNPLMKGSYTCYRPGQFTTMAGLEGVPA
                     GNLFFAGEHANSFYEWQGFMEGAALSGIAAAKSILTTVKIH"
     gene            2939..3160
                     /locus_tag="VFT30_01010"
     CDS             2939..3160
                     /locus_tag="VFT30_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEU5091230.1"
                     /translation="MKLIPVHSSGIRAVGYEEGREILSIQFIDGDLYEYYEVPASEVI
                     DLFQAESIGWFVNKRIKPQYKYRKLKAAS"
BASE COUNT          751 a          883 c          947 g          718 t
ORIGIN      
        1 cagggtttct tgttttgccg gatccgacat gcaatctgtt tcaaatctca atccgtaaat
       61 tttcaccgca ctctttctcg cgtactgtag atcgtcagcc gtcaggccga gcgacttttt
      121 atcggcatcc gtgtaccacc agaaacggag gggcagggca ggttgggcga ccaccgtggc
      181 actgacgcga ggatgattca gcaacgctaa aggaataggg cctgtcaggc agttgccgat
      241 gatgccgatg tgctgttgag catgccgctc tccaaccttc tgcaccacgc cccgcaacca
      301 gttcacaaca ggagcgcttc cctgagacgg gacaccccat tcaccgctgg ggaaaaagtc
      361 catcaatcct cggaaccaat aggcccacaa tcctttcgta agggaaaact ttcctttctc
      421 gccgaatagc agcggcacat acaccgtgaa gtccttcgag agctcctcgg cgtaggcaag
      481 tgtcccagga gacagtccgg tcaactcgtg aagcaaaagc actggaggtt tgtcggttcc
      541 gtcatgtagg cagaacaccg aatgggtaat gccctcgtgc gagaatggtt ggtcgaaaca
      601 ctctgcccaa ttccgagtag gcataccttc ttctttcgta tgcggcgcgc acatccccag
      661 ctgcgtcgcc ccacaacctg cctgaaataa gagtaacgcg gtgacgatgg tttgcgggag
      721 gaagtggcgc ggtttgtcca tattgtgttt ccggcgtaat gtgctgcggc tcttgtcatg
      781 catgctgtga aataaatcag cagaagattg ccagcgtctg aggatcggac gcaagaccct
      841 taaggttcag atgcacagtt cgcgagtgga agggtattct actaggtatc tggtcgtgac
      901 ttccaagccg ggctcggcgc gctactgatc acattggtga ttgctggtgg catgctcgac
      961 ttctcgagcg agcgggggga ctgacttgtt caagcgactg cgcgagcggc ttggtgcggc
     1021 cctggatgat catcagtttg tgacctatct cgataagctt gcttcaccct gcactggttc
     1081 aaccggcaaa aatttctctt gagagacacg aagtctattc cgagggctac gatcgtgaat
     1141 tactgcaatg accgctaatg aattttgact gttgtcagaa ttgatttggc tgccgcaata
     1201 ccggagagtg ccgcgccttc cataaagccc tgccactcat agaacgagtt ggcgtgttcg
     1261 ccggcgaaga agaggttgcc cgcggggaca ccctccagac cggccatcgt tgtaaactgg
     1321 ccgggacggt agcaggtgta gctccctttc atcaggggat tcgatggcca atgttcaaga
     1381 tgcgcgagat gctgtccttg cacgagcgtg gcggctccga gcgcgcccgg atagatgagg
     1441 ttcaagtcct gtaagaaccg ctgagcttcg tcctgcactg cgtcgggatc cagtcgagta
     1501 cctcgcggtc cgcttgaata gtcggtcaga atccctttcg tttccgatcc taaggtcgga
     1561 tttgtttccc atgtcgtctg ggcatgcgcg agatcagtca cataggcggt tccgtttcct
     1621 cccaacgcgc gccacggccg gctggaaaag ccgatcatca tcttggcgtt ggtaccgtac
     1681 cccaatcctt gaatcgtggc gcgctgcgcg ggacggagtc cgaggccggc atctagatct
     1741 acgtgacgga gagtggtaaa ggggatggtc agcacggcga catcgtgcgt gaaggtcata
     1801 gtgcgggagc cttgttgaaa ggtcaactcg atgcggccat cgctcaggcg gcgaacccgc
     1861 acaagcctgg agtcgtacgt gatttgccct ggaagttcac gtgtcaatcc ttcgataatc
     1921 cgatcgttgc cgtcgactag atgataccgt tcatcgctga agacgccgaa aggggtaaac
     1981 ttcgaacgac ggtcagcatg tatgaacaac agaaacgtga ggcagctctg ctctcccgca
     2041 gccaacccat attcggcttc ataggcggca atgatggcag ccttggcgag tggcccagcc
     2101 gggatgccgg ctccgttgtg tccctcaaga taggtcaaca gattcgtacg gtccaaagct
     2161 atatcgtccg gtgtatgaga agtggccgtg acttctcgcg acaggcgatt caaatcggtc
     2221 cgcattatcg agacgaaatc tcgaaattcc gccacgactg cggcttctga atatccttgt
     2281 ccggagaagt agtagaagat ttctccaggc tccttgttca catcttcgag gctgaggtgg
     2341 aaccgtttcg cataacccag catcgtctta tgaagattgt cgatgagctc gccgccgcgt
     2401 tccgcaacct gaccaggaaa gaagcctcgt aacgaccagc agcggccgcc ggctcgcgta
     2461 gccgcttcgt agacagaggc tcggatgccg cgtgtcttga gtgtatcggc gcaagccagt
     2521 cctgcaagcc ctgcacccac gatgccgact gacagcgagg aaggcaatgg tttggccgca
     2581 gtggctcgat gaggaaaacc gggaatcgta ctaatagcga gtgccgctgc agtcattccg
     2641 gctctgagca gaaagtcccg gcgtgacagc acatcatggg tcgggtatga ggccgactct
     2701 tgggtgactc cgagagcctc agtggccgga caatgccgac catccgcgag caacgcggcg
     2761 gtcatcattt gggcaaatga acggaacgcg gcggttcgac tcattagcaa gcttcccatt
     2821 cgggaacagt cagggagcgg tatcttacaa gatagaagcg ccggcgaaaa gaggcatgag
     2881 cgtgctctcg cttgaatgcc gatcccttgg atggtggaat ggagtgagga ggcgacacat
     2941 gaaattgatt cctgtccatt cttccggtat cagggctgtc ggctatgaag agggacgaga
     3001 gatcctttct atacagttta tcgatggaga cctctatgaa tactatgagg ttcctgcgag
     3061 cgaagtgatc gatctgttcc aggcggaatc catcggttgg tttgtgaaca agcgaatcaa
     3121 accccagtac aaataccgaa aactcaaagc tgcgtcatga tcgcgtgccg cctcggatca
     3181 aagcatcgcg aaacgcgaaa acggttcatg gagagtgata ccgtacgcga aagcgtaaca
     3241 aacgaagatg ccgagaccgc caacagtacc cgcgaagagt ggcaaatctt ggcagcgcc
//
LOCUS       DASTWE010000092         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1489035, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000092 DASTWE010000000
VERSION     DASTWE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489035"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFT30_01015"
     CDS             complement(<1..539)
                     /locus_tag="VFT30_01015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5091231.1"
                     /translation="MTAQQRIVESFLCGAILFGLHTLTGCQTAQTETYGGGHRKAEAL
                     ASKEVKQAMAATKSRADKDMAAVLTGLEDLKPKPIISLSAEEARAQPTTADAVAALLE
                     ERKQSTAPMPIGKTKNQTIPGPGGPIPIRIYTPQGNGPFPVIVYFHGGGWVIATIDTY
                     DSSARALAKTADAIVVSVE"
     gene            complement(581..1654)
                     /locus_tag="VFT30_01020"
     CDS             complement(581..1654)
                     /locus_tag="VFT30_01020"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5091232.1"
                     /translation="MNIRLKPLKDQVIVVTGASSGIGLVTARLAAKRGARLMLIARNE
                     TALQQFTEKINSESSKSYIATFVAGDVAEESVARSAAEEAIRRFGRIDTWINCAGISI
                     YGRLVDVSLEDQRRLFETNFWGVVIGSRVAVEYLRQEGGALINIGSILSDRAIPLQGT
                     YCASKHAVKAYTDALRMEVEHDGLPISVTLVKPGSIDTPYAEHAKNYLPNEPKNASPT
                     YAPEVVAETILYCAEHPVRDVFAGGGAKMVSMLGNLAPRLADKIMKNTMFSVQQSNQV
                     DSDRQDNGLYGPARGLQERGGRALYVAESSLYTQASLHPMVTGVVAALAGAVLISVLR
                     REDSNGQSRRMRPMRSRTNEDFW"
     gene            complement(1928..2476)
                     /locus_tag="VFT30_01025"
     CDS             complement(1928..2476)
                     /locus_tag="VFT30_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU5091233.1"
                     /translation="MYRTLFAGLCMIVALGFFTGILGAQQDVQSDPKGEKLDPKADTQ
                     TFLTIAVEEQQVAIALGQLAAQRAKNNQVKEFGSKMVEDHKKVRREAEQLAAKHHVSL
                     PTKLTPEDKQKVDELSQLSGHEFDRAYMNYALQNHETTLEEFQHHLNTMRYPDLREWF
                     TSTLPALKAHRDQARLVKNSLQ"
BASE COUNT          540 a          730 c          685 g          659 t
ORIGIN      
        1 tattccactg acaccacgat ggcatccgca gttttcgcga gcgcccgcgc cgacgaatcg
       61 tatgtatcga tcgtcgcaat cacccatcct cccccgtgaa aatacacgat cacgggaaac
      121 ggaccgttgc cttggggagt atagatgcgg attggaatag ggccccctgg accggggatc
      181 gtttgattct tggtctttcc gatcggcatg ggagccgtac tttgctttcg ctcctccagc
      241 aatgccgcca cggcatcggc agtggtgggt tgtgctcgtg cttcctcggc ggagagcgaa
      301 ataataggct tgggttttaa gtcttccaac cccgtcagca ccgcagccat gtctttatct
      361 gcccgtgact tcgtggcggc catcgcttgt ttcacttcct tggaggctag agcctcagct
      421 tttcggtgtc cgccgccgta ggtttccgtc tgcgccgttt ggcaacccgt aagtgtgtgg
      481 agaccgaaga gaatggctcc gcagagaaat gattccacga tacgttgttg cgcggtcatt
      541 gtcgcttcct ttcttttttg aactcgccgc tgtccgacta ttaccaaaaa tcttcatttg
      601 ttcgacttcg catcggtcgc atgcgacgac tctgtccgtt cgaatcttca cgtcgaagga
      661 cggagatcag aaccgcaccg gcgagagctg cgactacccc tgtcaccatc ggatggagcg
      721 atgcttgagt gtagaggctg gattccgcaa catagagcgc ccgaccacca cgctcttgca
      781 atcctctcgc cggaccgtag agaccattgt cttgccggtc tgaatcgact tgattgctct
      841 gctggacgga aaacatcgta tttttcatga tcttgtcagc cagtctcggt gccagatttc
      901 caagcatcga caccatcttg gctccgcctc cggcgaagac atccctcacc ggatgctcgg
      961 cgcaatacag aatcgtctcg gcaaccactt ccggcgcgta agtgggagac gcatttttcg
     1021 gttcattcgg taaatagttc ttcgcatgct cggcatatgg agtgtcgatg gaaccgggct
     1081 tcaccagcgt aaccgagatc ggcagtccat cgtgctccac ctccatacgc agagcgtcag
     1141 tataggcttt caccgcgtgt ttactggcgc agtacgtccc ttggagtgga atggcgcgat
     1201 cggagaggat gctgccgatg ttgatgagag ctccgccttc ctgacgaaga tactcaacgg
     1261 cgacacgtga gccgatgacg actccccaaa aattcgtctc gaacaatcgg cgttgatctt
     1321 ccaaagacac gtcgacaagt cgtccataaa tcgagatccc tgcgcaattg atccatgtat
     1381 cgatccggcc gaagcggcgg atagcttcct cggccgcact ccgcgccacc gactcttcgg
     1441 ccacatctcc cgcgacaaag gtcgctatgt aactcttcga cgactcactg ttgatttttt
     1501 ccgtaaactg ctgaagcgcc gtctcatttc tggcaatcag catcaaacgg gcaccgcgtt
     1561 tagcggctag gcgagccgtc acaagaccga tgccgctgga agcaccggtg actacgatga
     1621 cctggtcctt caagggtttg agtctgatgt tcatgggtat ctagacgccg tcacggccgc
     1681 gttgcaatac gctgtgtgac aacatcatct tcttttttac cgccaatgag agtagccggc
     1741 gactagaaga ttcccgagaa atgactgcct tttgcagata gagttgagca aagggaagag
     1801 catgagcggg gatgcgactc agccgcgttg atccgtagcg caattcacaa agggagtcac
     1861 ccccgcctgc tcgtggatgg attttcttaa gcggtggctc ttatcacagc ctttacaagt
     1921 tatagactca ttgcagagag ttttttacca gccgtgcctg atcccgatgg gccttcaagg
     1981 ccggcaaggt ggaagtaaac cattcccgca ggtccggata tcgcatcgtg ttgagatggt
     2041 gctggaattc ctccaaggtg gtctcgtgat tttgcagggc gtaattcatg taggcgcggt
     2101 cgaactcatg accggacaat tgcgacagtt catctacttt ttgcttgtcc tcgggggtga
     2161 gtttcgtagg caaggacaca tgatgtttcg ctgccaactg ttctgcctct cgacggacct
     2221 tcttgtgatc ttcaaccatc ttggagccga attcctttac ctggttgttc ttggcccgtt
     2281 gtgcagccag ttgacccagc gcgatcgcga cttgctgttc ctcgacggcg attgtgagaa
     2341 acgtttgcgt atctgctttg ggatctaatt tttctccttt aggatctgat tgcacatcct
     2401 gttgcgcccc tagtatccct gtaaaaaagc ctagggcgac aatcatgcaa agacctgcga
     2461 atagcgtacg atacatggga tgctccttgg ataaggtgag tagcctttgg atctacacgg
     2521 ttccctagta ttctcgccaa tgcccggtgc aattcatgga cccgggagtt tggcttcccc
     2581 cctacctcgt caaggccacg cactgaggac ctta
//
LOCUS       DASTWE010000093         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1230494, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000093 DASTWE010000000
VERSION     DASTWE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230494"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFT30_01030"
     CDS             complement(<1..337)
                     /locus_tag="VFT30_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5091234.1"
                     /translation="MAQTDTQRIDISPAQEPLQSVEPCTLVIFGGSGDLARRRLIPAL
                     YNLLLDGLLPSNYVVLGLGRTPMNDEEFRSTVRDGVIKHSRQALIEETWSAFSQHLFY
                     LAGGNDDAQT"
     gene            complement(417..>1136)
                     /gene="gnd"
                     /locus_tag="VFT30_01035"
     CDS             complement(417..>1136)
                     /gene="gnd"
                     /locus_tag="VFT30_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU5091235.1"
                     /translation="VPRVVWVMVPSGAPTEETIQAVAALLQPGDIIVDGGNTRFHDDV
                     RRAAELKKKGIHYVDAGTSGGIWGLKVGYCLMVGGEEAAVKRLAPALKTLAPENGWAH
                     VGSVGAGHYVKMVHNGIEYSMMQGYAEGFELMSKSEYKLDLARVADLWMHGSVVRSWL
                     LELAASALKDDQKLEKLKGYVQDSGEGRWMIADAIEKDVPVPTLTTALFTRFRSRQEE
                     SFAEKMLAALRNAFGGHAVRR"
BASE COUNT          235 a          349 c          298 g          254 t
ORIGIN      
        1 aggtctgcgc gtcgtcgttt ccccctgcca ggtagaacag gtgctgggaa aaggcgctcc
       61 acgtttcttc gattaaagcc tgtctggaat gtttgatgac accatcgcgg acggtcgacc
      121 ggaattcttc atcattcatt ggagtgcggc ccaggccgag cacgacgtag ttcgacggca
      181 gcaatccgtc gagcaataaa ttatagagag caggaatgag gcgtctccgg gccaaatctc
      241 ctgagccgcc gaatatcacc aacgtacaag gttcgacgga ctgcagcggt tcttgggctg
      301 gactgatgtc gatccgttga gtatccgttt gagccatcag gatctcccta gcagcgtgtt
      361 gagaaagtcc gccggcgtcg aagcgcgttc aaccgcatgt ctctgatacc gggctgttat
      421 cgccggacgg cgtgcccgcc gaaggcattc cgcagggccg ccagcatctt ttcggcaaat
      481 gattcttcct gccgcgatcg aaatcgcgtg aagagcgccg tcgtgagggt gggaaccggc
      541 acatcctttt cgatggcatc ggcgatcatc caacggccct ctccggagtc ttgaacatac
      601 ccttttagtt tttccaactt ttgatcgtct ttgagcgcac tcgcggccaa ctccaagagc
      661 cacgatcgga cgacactccc gtgcatccat aagtcggcca cgcgtgcaag atcgagcttg
      721 tactcgctct tcgacatcaa ctcaaatccc tcggcatagc cttgcatcat gctgtactcg
      781 atgccgttgt gtaccatctt cacataatgt ccagcaccga ccgagccgac gtgggcccaa
      841 ccattttcag gcgccaaggt cttcaacgcc ggtgcaagcc gtttgacggc agcctcttct
      901 ccgccgacca tgagacaata gccgactttc agtccccaga tccctccgct ggttcccgcg
      961 tccacatagt ggataccttt tttcttcagc tcggcagccc gccgcacatc gtcgtgaaac
     1021 ctcgtgttcc cgccatctac gatgatgtcg ccgggttgca agagtgcagc cacggcctga
     1081 atggtttctt cggtaggagc tccggaagga accatcaccc aaacgacacg cgggac
//
LOCUS       DASTWE010000094         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1069137, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000094 DASTWE010000000
VERSION     DASTWE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069137"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFT30_01040"
     CDS             complement(<1..499)
                     /locus_tag="VFT30_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5091236.1"
                     /translation="MKIGLISDTHIPESMPELPAQVRTVFAGADLILHAGDLHCLDVL
                     NWLEEIAPVLACRGNGDEGSGGREVVPEDPRLRDAVVTNSAGFRIGMVHDVLDPHEYP
                     KWPLSRTMEMNFGGPTDLIVCGHSHVESIKRYDNVLVINPGSPTFPHNYSAQPGTVAV
                     LTLEES"
     gene            625..>1161
                     /locus_tag="VFT30_01045"
     CDS             625..>1161
                     /locus_tag="VFT30_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5091237.1"
                     /translation="MSEVIETRLPVTTLTEEEVLFREATRDFAEKRVKPQVMDMDRAG
                     KLPRELIDELFTLGVMGIEIPQSYGGAGSNFLMSILAIEELARVDPAVAVCVDVQNTL
                     VNNALLRWLTPEQQARFFPRLARDTVGAYALSEPDSGSDAFGMATQAKLDGDSFVLNG
                     RKMWITNGAEAGVYLLFAN"
BASE COUNT          255 a          320 c          320 g          266 t
ORIGIN      
        1 gactttcctc tagtgtgagt acggcaacag ttcctggttg ggcactgtag ttgtgaggaa
       61 aagtcggact gccgggattg atcaccaaga cgttgtcgta tcgcttgatc gactcgacat
      121 gcgagtggcc gcagacgatg agatcggtcg gtccgccgaa attcatttcc atagttcgcg
      181 agagtggcca cttcgggtac tcatgaggat cgagaacatc atgcaccatg ccaattcgga
      241 atcctgcgct gtttgtgaca accgcgtcac gtaagcgcgg gtcctcggga accacctccc
      301 gtccaccgga tccttcatcg ccgttgccgc gacaggccag caccggggca atctcctcta
      361 accagttgag gacgtcaagg cagtgcagat ctcccgcgtg taaaatcaaa tcggcacccg
      421 caaaaaccgt tcgcacctga gcaggcaatt cgggcatcga ttcgggaata tgggtgtcag
      481 aaatcaggcc gattttcatc gcttcttctc cgcctctctc gctatcccta tcttgtctct
      541 gtcttcacga actatatcgt ctagacagtg ccggagcaac gtgaaatggt tgcacagtct
      601 ctcaccaaat agggaggtgg agctatgtct gaagtgatag aaacgcgcct gccagttact
      661 acgctaactg aggaagaggt gctgttccgc gaagcgacac gtgacttcgc ggagaagagg
      721 gtcaaaccac aggtgatgga tatggaccgt gcgggcaaac tgccgcgcga actgatcgac
      781 gaacttttta ccctgggagt gatgggtatt gagattccac agtcctacgg tggggcgggc
      841 tcaaatttcc ttatgtcgat cttggcgatc gaagaactcg cccgcgtcga cccggcagtc
      901 gcggtgtgcg tcgatgtgca aaacaccctc gtcaataatg ctctcttacg ctggcttacc
      961 ccagagcagc aagcgcgatt tttccctcgc ctggctcgcg atactgtcgg tgcgtacgcc
     1021 ctctctgaac cggattcggg aagcgatgcg ttcggcatgg cgactcaagc taagcttgac
     1081 ggtgactctt ttgtcctcaa tggccgcaaa atgtggatca ccaacggtgc cgaagccggc
     1141 gtgtatttgc tattcgccaa c
//
LOCUS       DASTWE010000095         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_422640, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000095 DASTWE010000000
VERSION     DASTWE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422640"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFT30_01050"
     CDS             <1..451
                     /locus_tag="VFT30_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091238.1"
                     /translation="EIQGIGFVGVKGFAGGFGDRLLQSWGESVLKRFVRESVDEALKL
                     ESALAKLTTSVRIVLMHYAPVFDTVIGESPEIIPFLGTSRLEEPVNRYGVTAVFHGHA
                     HHGTLEGRTREGVPVYNVALPLLRLAAPAQAPVKIIQVPIPRHAESV"
     gene            448..>1289
                     /locus_tag="VFT30_01055"
     CDS             448..>1289
                     /locus_tag="VFT30_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU5091239.1"
                     /translation="MNYRGMPSDKTSPSIYRLALQDMQRSGVPFLVGGAFALESYTGV
                     IRRTKDLDVFVLRQNVDQLLRFLACVGYETEVRFPHWISKAHRDDYVIDIIFGSGNGI
                     CPVDEKWFGAAPIATVMGERVRLVPVEEMIWSKAFIMERDRYDGADIAQLMHARSEDM
                     DWARLLWRFDRHWRVLFSHLVLFGFVYPGRRLQIPHWLMREMMQRLNLEMQTLSSDRD
                     VCQGTLLSWGQYLNHIEQGDYEDARHSPRGSLTACDTRFVTERFQEEQKGSPGVQTPA
                     AGSER"
BASE COUNT          279 a          318 c          398 g          294 t
ORIGIN      
        1 ggagatacag gggataggat ttgtcggagt gaaagggttc gccggtggat tcggagatcg
       61 gcttttgcag tcatggggag agtcggtgct caaacgattt gttcgtgagt cggtggacga
      121 agcgctgaag ttagaatcgg ctttggccaa actgacgacc tcggtccgaa tagtgttgat
      181 gcactatgcg cccgtattcg ataccgtcat aggagaatcc ccggaaatca ttccgttcct
      241 tggaaccagc cgcctggaag aacctgttaa tcgctacggc gtgacggcgg tatttcatgg
      301 acacgcacat cacggcacac tggaaggacg aaccagagag ggtgtgccgg tgtacaatgt
      361 cgcgcttccc ctgcttcgat tggctgcacc ggcgcaggcg cccgtgaaga tcatccaggt
      421 tccaattcca cgacatgcgg agtcggtatg aactatcgcg ggatgccgtc cgataaaaca
      481 tcgccgagca tctatcggtt ggcgctccaa gatatgcagc gttccggagt tccttttttg
      541 gtgggcggcg catttgcgct ggagagctac acaggtgtga ttcgccgcac caaggatctc
      601 gatgtgttcg tgctccgaca gaatgtggat caactcctgc gatttctggc ctgtgtgggg
      661 tacgagaccg aagtgcgatt tccgcactgg atcagcaagg ctcacagaga tgactatgtc
      721 atcgacatta tttttgggtc tgggaatggg atttgccccg tcgatgagaa atggttcggc
      781 gcagcgccga tcgcgacggt catgggtgag cgggtccgac tggtgcccgt ggaagaaatg
      841 atttggtcga aagcctttat catggaacgt gaccgttacg acggggccga catcgcgcag
      901 ctgatgcatg cgcggtccga agacatggat tgggcgcggt tattgtggag attcgatcgt
      961 cattggcgtg tactattcag ccatcttgtc ttgttcggct tcgtctaccc ggggcgccgg
     1021 ttgcaaatac ctcattggct gatgcgtgaa atgatgcagc ggctcaattt ggaaatgcag
     1081 acgttgtcgt ccgatcgtga tgtctgccag ggaacactgc tctcctgggg acaatatctc
     1141 aaccacatcg agcaaggtga ctacgaagat gcccgccaca gtccacgcgg atcgctgacg
     1201 gcatgtgaca ccagatttgt gaccgagcgg tttcaggaag aacaaaaggg aagtccaggc
     1261 gtccagacgc ctgcagccgg ctccgaacg
//
LOCUS       DASTWE010000096         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_34982, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000096 DASTWE010000000
VERSION     DASTWE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34982"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFT30_01060"
     CDS             complement(<1..314)
                     /locus_tag="VFT30_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5091240.1"
                     /translation="MIELYDLVGANNLRFSPFCWRTKAVMAYKKIPYTTVPIRFSDKD
                     KLAFSGQDRVPVIKDNGKVVSDSWTIAEYLEDTYPDAKIFPGLGLKEACRFFNLYVDR
                     TV"
     gene            complement(311..>1018)
                     /locus_tag="VFT30_01065"
     CDS             complement(311..>1018)
                     /locus_tag="VFT30_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5091241.1"
                     /translation="LVTEALRCGVNLFDTAPMYGAAENVLGEVLKGRRRDIIVATKVL
                     AYDQRSARTLIEESFRKLQIDVIDLMQIHNMSAWREVTPVLEEYKAKGRIRFLGITDY
                     RTSMFPEMMQAMRTGKFDTIQIPYNLGDRAAEREIIPLAKQLNLGVLVMTPIAPIFDR
                     NRLLQPLQRQDLAFLTPYGVTTPGQALLKYLLTNADVATLLPATSRLERVTENSKSAD
                     GPGLPPEVLRRLEGMLL"
BASE COUNT          226 a          264 c          283 g          245 t
ORIGIN      
        1 tgaaccgtac gatcgacata gagattgaag aagcggcacg cctctttcag tccgagaccg
       61 gggaaaattt tggcgtccgg gtacgtgtcc tcaaggtatt cggcaatggt ccaagagtcg
      121 gagacgacct tgccgttatc cttgattacc gggacgcgat cttggccgga aaatgcgagc
      181 ttgtctttgt cggagaagcg aattggcacg gtcgtatagg ggatcttttt atacgccatc
      241 acggctttcg tccgccagca aaatggactg aaacggagat tattggcgcc aacaaggtcg
      301 tatagctcga tcatagcagc atcccttcta gccgacggag cacttcgggt ggtaatcccg
      361 gtccgtctgc actttttgag ttttctgtaa cgcgttcaag ccgcgaggtg gcaggtaaca
      421 gcgtggcgac gtcggcgttc gtgagcaaat acttgagcaa ggcttgcccg ggtgtcgtga
      481 cgccgtacgg ggtaaggaac gccaaatctt gacgctgcaa tggttgtaac aagcggtttc
      541 gatcaaaaat tggggcgata ggagtcatca ccaacacgcc gaggtttaac tgcttggcaa
      601 gtggaatgat ctcccgttcc gccgcgcgat ctccgagatt gtacggaatt tgaatcgtgt
      661 caaatttccc cgttcgcatt gcctgcatca tctcaggaaa catgctggtc cggtagtcag
      721 tgatcccgag gaaacgaatc cgccccttcg ctttgtattc ttcgagaacg ggagtaactt
      781 cccgccaggc agacatgttg tgaatctgca tcagatcgat aacatcaatc tgcaacttgc
      841 gaaacgattc ttcgatcagt gtccgcgccg agcgttgatc gtaggcaagc actttcgtcg
      901 cgacgatgat gtccctgcgc cttcctttga gcacttcccc gaggacgttc tctgccgcac
      961 cgtacattgg cgcggtatcg aacaggttca cgccgcagcg tagtgcttcg gtgaccag
//
LOCUS       DASTWE010000097         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1101412, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000097 DASTWE010000000
VERSION     DASTWE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101412"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFT30_01070"
     CDS             <1..792
                     /locus_tag="VFT30_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEU5091242.1"
                     /translation="TVNRAMSKSMSKSHLVAMLACVAFLVPTQEKLPKVLFYSDPQRS
                     DNDIVRRTQPEVPSPAEKRFVEITKGIFAVTATQDASEVSREKLKKYRGVVFFTAINP
                     PADKEALVEWVRDGGAFTGIHSTANTFQSYAPFGEMLGAYFESRPWRTKDKPLAKVRV
                     KVEDKRHPATKHLGDAFEIEDDLYLFRNWDRNQVHVLLSMDPMSLDMTKVERENPDMA
                     IAWTKTYGKGRVFYTALGDPEHVWKDARYQTHLIEGVKWTMGKLP"
     gene            complement(1029..>1594)
                     /locus_tag="VFT30_01075"
     CDS             complement(1029..>1594)
                     /locus_tag="VFT30_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEU5091243.1"
                     /translation="CSIMLLDEKKEELVIKATQALSHAYSSKPNIKVHKSLLGRVVID
                     RKPLVIRDVTLEKGYTYPELAKQEGLRSLVSVPLMIKNSVIGVFNLYTSQERQFSNEE
                     IQLISTVANQAAVAIENTKLMAETLSMQEALQTRKVVERAKGLLIKEEKITEDEAYRR
                     IQQKSMKLRKSMREIAEAIVLAAEIKM"
BASE COUNT          349 a          443 c          446 g          356 t
ORIGIN      
        1 acagtgaata gagcgatgag caaatccatg tctaaatctc atctcgtggc catgctcgcg
       61 tgcgtggcgt tcctggtccc gacgcaggag aagctgccga aagtgctctt ctactccgat
      121 ccacaacggt cggacaatga catcgtccgc cggacacagc cggaggtgcc ctcgcccgcc
      181 gagaagcgtt ttgttgagat aacgaagggc atattcgcag tgaccgccac ccaagatgcc
      241 tcggaagtga gccgggagaa gctgaagaag tatcgcggcg tggtattctt caccgccatc
      301 aatccgccgg ccgacaaaga agcgctggtc gagtgggtta gagacggcgg cgcatttacc
      361 ggcattcatt ccacggcgaa cacgtttcag agttacgcgc cgttcggcga gatgcttggg
      421 gcgtacttcg agagccgccc ctggcgcacc aaggacaagc cgcttgcgaa ggtgagagtg
      481 aaagtggagg ataagcggca cccggcgacg aagcacctgg gtgacgcgtt cgagatcgaa
      541 gacgacttgt atctttttag aaattgggat cgcaaccagg tgcacgtcct gctcagcatg
      601 gacccgatga gcctcgacat gaccaaggtg gaacgcgaga atccagacat ggcgatcgcc
      661 tggaccaaga cttatggcaa agggcgcgtg ttctatacgg cgctgggcga tccggagcat
      721 gtgtggaaag acgcacgcta ccagacgcac ctgatcgaag gcgtgaagtg gacgatgggg
      781 aaattgccgt agcgagtcag aatgagtaga tagcgagcca cgtcataccg ggcgctgtcg
      841 gtcggttctt gacgcttcac cctgccttca gccaccctct ccccatgaac gggagaggga
      901 gttctcgctg ggtggcgcag acatgcatca tctgcatctc tgcgaacagt cggtctgcag
      961 tgacactaac tcatcgtcga ctcaatagag ttgctattgt ctgatcagac tgatgagaga
     1021 caccgcccct acatcttgat ttccgcggcc agcacgatgg cttcagcaat ctctcgcatg
     1081 gacttgcgca gcttcatgct cttctgctga atgcgccgat acgcttcatc ctcggtaatc
     1141 ttttcctctt taatcagaag tcccttcgcc cgctccacga cttttcgtgt ctgcagcgct
     1201 tcctgcatcg aaagcgtctc cgccatcagc ttagtgttct cgatcgccac agccgcctga
     1261 ttcgcgaccg ttgagatcaa ctggatctcc tcgtttgaga actgtcgttc ctgcgaggtg
     1321 taaagattga acactccgat cacactgttc ttaatcatca agggaaccga gaccaacgag
     1381 cgcagccctt cctgtttggc tagttccgga taggtgtatc ccttttcgag ggtgacatct
     1441 cggatgacca gcggcttgcg atcgatgacc acacggccca gcaaactttt gtggaccttg
     1501 atgttcggtt tgctggagta ggcatggctt aaggcctgag ttgctttgat taccagctct
     1561 tccttctttt cgtccagcag catgatggag caga
//
LOCUS       DASTWE010000098         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_875147, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000098 DASTWE010000000
VERSION     DASTWE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875147"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFT30_01080"
     CDS             complement(<1..658)
                     /locus_tag="VFT30_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091244.1"
                     /translation="MTELKLPEELEDQAILHAFGILEPDDRRAFMVRLQGDSDLLRQA
                     VTAYQATTDALASAVAPVTPSATLRERLVKKVALEAGRETEQFEHAANTLALASLPVK
                     PRDSVRERLLSRIEGHSDVRLDVTDSAYVLSETPALVGNGGERRLSQEVDSFSTRLRS
                     CWMGVSNFLRTLVIRSMTSARVRYWKDKIAFQQPTKGLTFIRASEGTWRGIAPGVMAK
                     L"
     gene            complement(655..1254)
                     /locus_tag="VFT30_01085"
     CDS             complement(655..1254)
                     /locus_tag="VFT30_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5091245.1"
                     /translation="MLLMSETTKAAQDQEWTQLIALTAQGDQTALATFYDRTSPQVFG
                     LIYKILNNREAAEEVTLDVYTQVWRQAHTYDQARGAPGAWLMMLARTRAIDRFRAGAV
                     EQGRIESLDAVELFISDGDTPEQDVEGQERRRYVQQALAVLTAEQRQVIALAYFYGLS
                     QSEIAEKLQLPLGTVKTRIRLGMMKLREALAPYEAGLTP"
     gene            1497..1970
                     /locus_tag="VFT30_01090"
     CDS             1497..1970
                     /locus_tag="VFT30_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEU5091246.1"
                     /translation="MAIKTAFHITLSMLLLTLVGCNTMEQASTSTMSDKPLYERLGGK
                     PAITAVVEDFVGRVAADDRINGKFANTDIPRLKMLLVEQICQASGGPCSYTGRSMKAT
                     HAGMGVSKADFDALVGDLVATLNKFKVPEREKNELLGALAPMKKDIVEKPQMSMN"
BASE COUNT          498 a          630 c          547 g          457 t
ORIGIN      
        1 gcaacttggc catcacgcca ggagcgattc cccgccaagt tccctccgat gccctgatga
       61 aggtaagccc cttcgtcggc tgctgaaatg cgatcttgtc tttccaatac ctgactcgcg
      121 ccgaggtcat agaacgaatg acaagagtcc gcaggaagtt tgaaacaccc atccaacaag
      181 atcgcagccg agtggagaaa gaatcgacct cttgagaaag gcgcctctcc cctccattac
      241 ccactagggc aggcgtttcg cttaggacgt acgccgagtc tgtgacatca agtcggacat
      301 ccgaatgccc ttcgatgcgg gagaggagcc gctcccgtac agaatcccga ggctttactg
      361 gaagggacgc caaggccaac gtattcgcgg cgtgctcgaa ctgctcggtt tcgcgtccgg
      421 cctccaacgc cactttctta acaagccgct cgcgaagagt cgcggaaggt gtcactggag
      481 ccacggctga cgccaatgca tccgttgtcg cttgatatgc tgtcactgct tgccgcaaga
      541 gatcggagtc tccttgtagt ctcaccataa acgcccggcg atcgtccggc tccaatatcc
      601 cgaaggcatg gaggatcgcc tggtcttcca attcttccgg cagtttgagc tcagtcacgg
      661 tgttaaaccc gcctcataag gcgcgagcgc ttcgcgcaat ttcatcatac ccaatctgat
      721 tctcgtcttc accgtaccga ggggcagctg cagcttttcc gcaatttcac tttgacttaa
      781 gccgtagaaa taggccagtg cgatcacttg ccgctgctcc gccgtcagta ccgccaacgc
      841 ctgttgtaca taccgtcgac gttcttggcc ttcgacatcc tgctcgggtg tgtcgccgtc
      901 gctgataaac agttcgacgg catccaacga ttcgatgcgc ccctgctcga ccgccccggc
      961 gcgaaaacga tcaatcgccc ttgttctggc caacatcatc aaccaagcac caggcgcccc
     1021 tcgcgcttga tcatacgtat gggcctgacg ccacacctgc gtgtagacgt ccaaggtgac
     1081 ttcttcagcg gcttcgcgat tattgagaat tttgtagata agcccaaata cttggggact
     1141 cgtccgatca tagaacgtgg cgagcgctgt ctgatcaccc tgtgctgtca gagcaatcag
     1201 ttgtgtccat tcttggtctt gagccgcttt ggttgtttct gacatgagca acatacaatc
     1261 tcccccatca aactatacga gtgaataggg gagattggat tgctgattgc gggtttttgc
     1321 accctcgata gaagccgaac tatagcacca taaaaatagc cccagcaagc agacctacct
     1381 cacgaagaga tccaaaccgg aacacgtctc gtaatggaga acagaccgcc ggtcagttct
     1441 gattcacgag acaggtgccc gacacaatca ttcagccatc agaggagaac agatcaatgg
     1501 ccatcaagac cgcgttccac atcaccctca gcatgctttt actgaccctc gtagggtgta
     1561 acacgatgga gcaggcatcc acatccacga tgagcgacaa gccgctctac gagcgattgg
     1621 gcggcaagcc ggctatcacg gctgtagtgg aggatttcgt cggtcgagtc gccgccgatg
     1681 accgcatcaa cggcaaattc gccaacacgg atattccccg cttgaaaatg ctgctcgtcg
     1741 aacagatctg ccaggcctcg ggcggaccct gttcctacac tggtcgcagc atgaaagcga
     1801 ctcatgccgg catgggtgtg agcaaagccg acttcgacgc gctggtcggg gatttagtcg
     1861 ccacattgaa taaattcaag gtcccggaac gcgagaagaa cgaactgctc ggggcactgg
     1921 caccgatgaa gaaagacatc gtcgagaaac ctcagatgtc catgaactga gaagtcagcc
     1981 aaatcgtcat gacgttttgg agaggaggta gtctatgaaa tcacgcgtcc atccatcctt
     2041 gatgttcgtc ttcactgtcg gactcttgtc cggctgcagc ggaagtgaca ccgtgcccat
     2101 cataactccg agtgggccca cagctacggc gg
//
LOCUS       DASTWE010000099         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_713705, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000099 DASTWE010000000
VERSION     DASTWE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713705"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VFT30_01095"
     CDS             complement(<1..792)
                     /locus_tag="VFT30_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEU5091247.1"
                     /translation="MADKTIKSDGIQKQTGESETGSSRRDFLVHSARVTAGAGIAALL
                     GNLGHWALSYAADKAPIKIGVLHSLSGTMAISEVSLRDVVLMAVDEINAKGGILGRQV
                     KPVVVDPASNWDLFAEKAKQLLLQDKVAVVFGCWTSVSRKSVLPVFEKNNGLLFYPVQ
                     YEGEECSHNVFYTGAAVNQQAVPAVEYLMSKEGGGYKKFYLLGTDYVYPRTTNKILRA
                     MLVAKKVPEANIMEEYTPFHHQDYQTIVGKIKKFAAGGGAAVISTI"
     gene            complement(825..>1174)
                     /locus_tag="VFT30_01100"
     CDS             complement(825..>1174)
                     /locus_tag="VFT30_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091248.1"
                     /translation="ALGVEFFRYGQDRNIGTGSEVRTLVGWGELDYAHDRDTTFIAGY
                     GFDNPSNSDLQGTVAVPDQQYLLNHRSYLTAVRHIWGDLFLSFEWNHLMTKWTTGESF
                     AGDNFMISTWYNF"
BASE COUNT          240 a          351 c          308 g          275 t
ORIGIN      
        1 gatggtgctg atgactgccg ctccaccgcc ggcggcgaac ttcttaattt tgccgacgat
       61 cgtttggtaa tcctgatggt ggaagggggt gtattcctcc atgatgttgg cctccgggac
      121 ctttttagcc acaagcatcg cacgcaggat cttgttcgtc gtccgcggat acacgtagtc
      181 agtgccgaga agataaaact ttttgtaccc tccgccttcc ttgctcatca aatattcgac
      241 ggcgggtacc gcctgctgat tgaccgccgc accggtatag aagacgttgt gagagcactc
      301 ttcgccttcg tactgcaccg gatagaagag caagccgttg ttcttctcga acaccggcag
      361 cacggatttg cgactgaccg aggtccaaca gccgaagacg accgcgacct tatcctgcag
      421 caggagttgc tttgcctttt cggcgaacag atcccagtta gaggcgggat cgacgaccac
      481 cggcttcacc tgccggccca aaatcccgcc tttggcattg atttcgtcga ccgccatcaa
      541 gaccacatcg cgcagcgaca cttcactgat cgccatggtc ccgctcaagg aatgcaggac
      601 gccgattttg atcggcgcct tgtccgctgc ataggagagc gcccagtggc cgagattgcc
      661 gaggagcgcg gcgatccccg ctcctgccgt cactctggca ctgtgcacga gaaagtcacg
      721 ccgtgacgaa ccggtttcac tctcgcctgt ttgtttctgt atcccgtctg acttgattgt
      781 cttatctgcc attgccggac tcctttcggt acgaaacacc gctgctagaa gttgtaccat
      841 gttgagatca tgaagttgtc gcccgcaaag ctctcaccgg ttgtccattt cgtcatgagg
      901 tgattccatt caaacgagag gaacaggtcg ccccagatgt gccggacggc cgtgagatac
      961 gaacggtggt tgagcaggta ttgctgatcg ggcaccgcca cggtgccttg caaatcggaa
     1021 ttgctcgggt tgtcaaatcc gtaacccgcg atgaacgtcg tgtcgcggtc atgcgcatag
     1081 tccaactcac cccatcccac caacgttctg acttcactgc cggtgccaat gtttcgatcc
     1141 tggccgtacc ggaaaaattc cacacccaat gctt
//
LOCUS       DASTWE010000100         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_455028, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000100 DASTWE010000000
VERSION     DASTWE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455028"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="exbB"
                     /locus_tag="VFT30_01105"
     CDS             <1..408
                     /gene="exbB"
                     /locus_tag="VFT30_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEU5091249.1"
                     /translation="LVDYGIIGLLLALSLWALAVGVERWLYYRRVAMNQFDNIQLMEM
                     ALTKRLVVIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVGAIMVGLSLALKATA
                     IGLLVAIPCVVLNNILRRRVSELLTLYKVQHGA"
     gene            398..772
                     /locus_tag="VFT30_01110"
     CDS             398..772
                     /locus_tag="VFT30_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU5091250.1"
                     /translation="MERELNQINVIPLVDVMLVLLVIVLTTATFISTGQVPVELAKAK
                     EVSDHQDVPLMITLTSDGRLFLNDQPVPQDGLTTVLNGHPRESAVVLRADRVTVLERF
                     VGVVDEVRGLGFKHVSLEVLRS"
     gene            769..>1404
                     /locus_tag="VFT30_01115"
     CDS             769..>1404
                     /locus_tag="VFT30_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091251.1"
                     /translation="MTTIEFNQISASAATEQARGWTASVLLHGVAVAGAFLLMAEIGK
                     PVLREPFQWDVSMVDAQTHAEPKQSESPLVEKVVQPPPSPVKPKPPLRQVTQPIAHQT
                     SQPTEHEAPVAVEPTKVVRDIAVNAEPVVEPTESQTVMSQVVMTAAIESSNESTIEQT
                     TPVESTFEHRTVQYRQIRYRETRADYGWLVDMVRKRVEDLMRYPMLAKANHW"
BASE COUNT          318 a          357 c          415 g          314 t
ORIGIN      
        1 ttggtggatt acggaatcat tgggctactt ttggcgctca gcctgtgggc cctggcagtg
       61 ggagtcgagc ggtggctgta ttaccgacgg gtggcgatga atcaattcga caatattcaa
      121 ctcatggaaa tggcgctgac aaaacgatta gtcgtgattg gaacggtagc cgcaaatgca
      181 ccctacatcg ggctgttggg cactgtgttg gggatcatgc tgacgttcca tacaatgggt
      241 acgtcgggaa cgatggccgt cggcgcaatc atggtagggc taagcctggc gctcaaggcg
      301 acggctatcg ggttgctggt ggcgattcca tgcgtcgtgc tgaacaatat tcttcgacga
      361 cgcgtctccg aactgctcac gctgtacaag gtgcagcatg gagcgtgagc tgaatcagat
      421 caatgtcatt cctctcgtgg acgtaatgct ggttcttttg gtcattgtct tgaccacggc
      481 cacgttcatt tcgacaggac aagtacccgt cgagctggcg aaggcgaaag aagtcagtga
      541 tcatcaagac gtacccttga tgattacctt gacgtccgat ggtcgactgt ttctgaacga
      601 tcaacctgtc ccccaggatg ggctgacgac tgtcttgaac ggtcatcctc gcgagtcagc
      661 ggtcgtatta cgggctgatc gagtcacggt cttggagcgg ttcgtgggag tggtcgatga
      721 agtgagagga cttggattca aacacgtgag ccttgaggtt ctgcgttcat gacgaccatc
      781 gaattcaatc aaatcagcgc gagcgccgct accgaacagg ctcgggggtg gactgcctct
      841 gtgctgcttc atggcgtggc cgtggcaggc gcgtttctcc tcatggcgga aattggcaag
      901 ccggttctgc gagagccgtt tcagtgggac gtttcaatgg tcgatgctca gacgcatgct
      961 gaaccgaagc aatctgaatc accgctggtg gaaaaggttg ttcagccgcc tccttcaccg
     1021 gtcaagccga aaccacccct tcggcaggta actcagccga tcgcccatca aacctcgcaa
     1081 ccgacagaac acgaggcgcc ggttgccgtt gagccgacga aggtcgtcag ggacattgct
     1141 gtgaacgcag agccagtggt ggaaccgacg gaaagccaaa cagtcatgtc tcaagtcgtc
     1201 atgacagcgg caatagaatc ctccaatgag tcgacgatcg aacaaacaac gcccgttgaa
     1261 tctaccttcg agcaccgaac ggttcagtat cggcagattc gctatcgcga aacccgagcg
     1321 gactatggct ggctcgtgga tatggtcagg aaacgggtcg aagatttgat gcgctatccc
     1381 atgcttgcaa aagccaatca ttgg
//
LOCUS       DASTWE010000101         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1456882, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000101 DASTWE010000000
VERSION     DASTWE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456882"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFT30_01120"
     CDS             <1..240
                     /locus_tag="VFT30_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091252.1"
                     /translation="TWGPSTYPIDAFVLAVVLLVSFFIFRDALAKQDEANDAILQAVT
                     TGEVEFPVIERILEIVAELDADLKEKNLKQKPRDI"
     gene            complement(669..>1056)
                     /locus_tag="VFT30_01125"
     CDS             complement(669..>1056)
                     /locus_tag="VFT30_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091253.1"
                     /translation="IAFRTDGRDDQVRGVKVAGNGTAYARGDLLPDQPRPRRPARVTD
                     VLRHHKVPGELLDPHLFTIYAYCEKIRVAIGVRDRHPRHDGRVEVQEALLPYNGRVRA
                     QGGTGEAPGWTPPVWLPERHGDHPRG"
BASE COUNT          238 a          304 c          299 g          215 t
ORIGIN      
        1 acgtggggac cttcgaccta tccaatcgat gcattcgtcc tagcagtcgt cttgctcgtc
       61 tcattcttca tattcagaga cgcgttggcc aaacaggacg aagctaacga tgctattctc
      121 caggcggtca cgaccggcga agttgagttt cccgtgatcg aacgcattct tgaaatcgtg
      181 gccgagcttg acgctgacct caaggagaag aatctgaagc agaagccaag agacatctag
      241 cctatgcaag ccccgcctga tccataatgc gtggcatatg cagggtgaaa gcaaacaatc
      301 actgtaccga tggatagacg taccgcctga cgcgaagcgt ccgactggcg cgagaaagca
      361 gatctccaag aactgcgtca caagaaccaa ttcacccaac agctccacga caagatcaag
      421 taccggatca agcggcggtc agaagctgag ggtcagtgtc agcgtgacca agcggagaag
      481 accaagaacg aatagcgtct ccttctcctg gcggcgcagg ccgaggtgct ggtaatgacg
      541 tccatacagc ctcatagtct tggggccgcg aggagcccgt ttgagtggcg ttaggtcgat
      601 aggacgatag tcggatcgag gagtgccccg ttaggacccg ctgagccatc tctcgaagaa
      661 tggccgcctc atcctcgtgg atggtcacca tgtcgctctg gtagccaaac gggcggcgtc
      721 caccctgggg cttcccctgt accgccttgc gcgcgcacac gtccgttata cggtaagaga
      781 gcttcttgga cttctacccg tccatcgtgg cgcggatgcc gatctcgaac tccgattgct
      841 actcgtattt tctcacaata ggcgtaaatt gtgaataaat gggggtcgag cagctcaccc
      901 ggtaccttgt gatgtctcaa gacatcggtg acacgtgctg gccgacgtgg tcgcggctgg
      961 tctggaagca gatccccacg ggcatacgcg gttccgttcc cggcgacctt cacgcctcgc
     1021 acctggtcgt cgcggccatc ggtgcggaac gcgatc
//
LOCUS       DASTWE010000102         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_810521, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000102 DASTWE010000000
VERSION     DASTWE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810521"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..496)
                     /locus_tag="VFT30_01130"
     CDS             complement(77..496)
                     /locus_tag="VFT30_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5091254.1"
                     /translation="MSEGNKQLIRRWFEEVWNKGRAESIDELFAENGIAHGLSDDPAK
                     PITGPRDFRPFHTLFREAFPNMMIVVEDVVAEGDKVAARCSVRAKHEGDFMGREATQA
                     PVEFTGMTIVRIDNGKIVEAWNNFDFMSLHKQVGLLA"
     gene            complement(515..901)
                     /locus_tag="VFT30_01135"
     CDS             complement(515..901)
                     /locus_tag="VFT30_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5091255.1"
                     /translation="MNNGFPAAVPEIPVTNMNVALDYYKDKLGFSIDWGGAEGGIAGI
                     SKGNCRMFLTDNDFRAHHGNAAPVMIWINLSSKNEVDELYEIWKKSAARIISPPESMP
                     WKLHEFTASDPDGNLFRVFYDFSRDT"
BASE COUNT          245 a          277 c          235 g          266 t
ORIGIN      
        1 tcaagattta catgatgtac aagataaatc ctgtaatcct gtaaatcttg taatcctgtc
       61 tacgagtgca cgccaatcag gcgagcagcc caacctgctt gtgcaagctc ataaagtcga
      121 aattattcca ggcctcaaca atctttccat tatcgatgcg cactattgtc atgcccgtaa
      181 actcgaccgg agcttgagtc gcctctcttc ccatgaagtc gccttcatgc ttagcacgca
      241 cagagcaacg cgccgcgact ttgtcacctt cagcgaccac gtcctcgaca acgatcatca
      301 tgttaggaaa ggcctcgcga aacagcgtgt ggaaaggccg gaagtctctc ggacccgtaa
      361 tcggcttcgc tggatcatcc gataagccgt gagcgatgcc gttctcggcg aacaactcgt
      421 cgatgctttc ggcgcggccc ttgttccaga cttcttcaaa ccagcgacgt attaattgtt
      481 tgtttccttc tgacatgagg tcctccgatg tagtttaggt gtctcgggag aaatcataaa
      541 acacgcggaa taggttgccg tctgggtcag atgctgtgaa ctcatgcagc ttccatggca
      601 tcgactcagg aggtgagatg attcttgctg cactcttctt ccaaatctca tatagctcat
      661 ctacttcatt cttgctactg agattgatcc agatcatcac tggcgcagcg ttgccatgat
      721 gcgcgcgaaa gtcattgtcc gtaaggaaca ttcggcagtt gccctttgag attccggcga
      781 tgccaccctc agcgccgccc caatctatgc taaagccgag tttgtcttta tagtagtcca
      841 acgcgacgtt catattggtc accggtatct ccggcactgc ggcgggaaag ccattattca
      901 ttggaccacc tccttgtccg ttgagattat taaaccacag attacacgga taaaggcgga
      961 ttcgaatcgt gtgatggaga cattatgaac acactccagg acattcgcta cgcattccgg
     1021 atg
//
LOCUS       DASTWE010000103         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_616688, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000103 DASTWE010000000
VERSION     DASTWE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616688"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFT30_01140"
     CDS             <1..345
                     /locus_tag="VFT30_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091256.1"
                     /translation="ETLNWDEEPERDQGQLFDPDAVSISPGDVNAPLYENHWPSAQAA
                     WDQLKDREVIRETTRQALDGRQVHVERGSFRGQHYLYCANTFDQRQLVIVEEVRSAML
                     DEYYQEIIRGLL"
     gene            524..>1128
                     /locus_tag="VFT30_01145"
     CDS             524..>1128
                     /locus_tag="VFT30_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091257.1"
                     /translation="MKAAHTTIIVALSLVCIGCTTPPEVKQALIDKDRIYAENERLMQ
                     QYRELVGNVTQRHQYWYRYVQTRFKLNLALQWATTNPKLTDVTDAELAKDDADLLGTE
                     IIALINEIRLTDLPERRGPSGQTVFQAGTGDMNHLLQKLPELIGRIEERVVKDARTSF
                     TVDLTAFDQYRTNVEAVRKINAIIKQYLDIDVTLSRNDVQSL"
BASE COUNT          280 a          300 c          305 g          243 t
ORIGIN      
        1 gagactctga attgggatga agagccggag cgggatcagg gacagctctt cgatccagat
       61 gccgtttcga tatcacccgg cgatgtcaat gccccgctct acgagaatca ttggccttcg
      121 gcacaagcgg cgtgggacca attgaaagat cgggaggtca tcagagagac cacccgccaa
      181 gcactagatg gccgacaagt gcatgtggag cgcgggagct ttcgcggaca acactatctc
      241 tactgcgcca acaccttcga ccaacggcaa ctggtcatcg tcgaagaggt tcgttcggcg
      301 atgctcgacg agtactatca agaaatcata cgtgggctcc tctaggaaag ctatcggctt
      361 gtcctgtcta cgtggctctc aataccataa atacccgata ttcctcttgc aatcatttat
      421 agccctcgct aaccttgggc cgctttccaa ctgattggag acagtaagca ggttgcgttc
      481 tcgtttgttc gtttccggcg tgagtcggga aggaggaatc actatgaaag cggcgcatac
      541 caccataatt gtcgcgctga gtctggtctg tatcggttgc acgactccac cggaagtcaa
      601 gcaggccttg atcgacaagg atcggatcta tgccgagaac gagcggctga tgcagcaata
      661 ccgtgagtta gtcggcaatg tgacccagcg gcatcaatac tggtatcgct atgtccaaac
      721 caggttcaag ctcaatttgg cgctgcaatg ggctaccacg aatcccaaac tgaccgacgt
      781 aaccgatgct gagttggcga aggacgatgc cgatctgctt gggacggaga tcatcgcgct
      841 gatcaatgag atccgactca cggatctgcc ggagcgcaga gggcccagcg ggcagactgt
      901 ctttcaagct ggcacagggg atatgaatca tctccttcaa aagttgccgg aactcatcgg
      961 ccgaatcgag gagcgtgtcg tgaaagatgc acgcacatct ttcacagtgg acctcacggc
     1021 attcgatcag taccgcacca acgtcgaagc ggttcggaag atcaacgcta tcatcaaaca
     1081 atacttggat attgacgtca cgttgtctcg caacgacgtg caatcgct
//
LOCUS       DASTWE010000104         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1327670, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000104 DASTWE010000000
VERSION     DASTWE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327670"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1174)
                     /locus_tag="VFT30_01150"
     CDS             complement(41..1174)
                     /locus_tag="VFT30_01150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU5091258.1"
                     /translation="MALNRIHKTLAAILCIICLTSCVQAVPPDLLVALEAIDQDLISL
                     RAPEAAPEEYGQFVRQWISLKAHIQLDDDLIRWPWESSDLENDLRQLYIMGERTVSQL
                     HERQTSQHRAAQAKVVRLEQQLHAITSRVESIDGRILLGEKVVQTDLLVKQARSFFEQ
                     KDFQRSMQAAEKADHALKAQALLLSRELGRYADESRIAYWQALAKQAIDWSRIHQSTA
                     IVVSKADRELTLYKGGRKVLSYSVRLGFNGMLEKQFQGDGATPEGRYHVTDKRGLGQT
                     QFYRALALDYPNAEDRRRFELAKRSGKIGPAKGIGGQIEIHGADNERLAQTLGCIMLD
                     NSNMAVLYEGVSVGTPVTIVGALTRENAVALALSELAQRREEI"
     gene            complement(1183..1794)
                     /locus_tag="VFT30_01155"
     CDS             complement(1183..1794)
                     /locus_tag="VFT30_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091259.1"
                     /translation="MLRKASLVSLALTFVAIAGCAEPPTQQIQEAEKALKDAQESGAA
                     TYSADEYAKLEGTLAALRKEVADQDEKFTLFRDYGKTQQLSASAKADSERIKAASVQK
                     KEEAKAAALQAQQVAEEAVKATQDLVAKAPVGKDRAAVEAIKNDVEGLKSLLKQVQAS
                     IDKEDYPAAQTQAKAIHDMSQGVSTEIQNALAKVGKGKPAKKK"
     gene            2262..2399
                     /gene="rpmH"
                     /locus_tag="VFT30_01160"
     CDS             2262..2399
                     /gene="rpmH"
                     /locus_tag="VFT30_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5091260.1"
                     /translation="MSFTFQQPSNLKKKREHGFRKRMSTKNGRKVLARRRAKGRARLT
                     V"
     gene            2510..2905
                     /gene="rnpA"
                     /locus_tag="VFT30_01165"
     CDS             2510..2905
                     /gene="rnpA"
                     /locus_tag="VFT30_01165"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5091261.1"
                     /translation="MGCGEETPFQMTPKAQGRNVIFLRRNRDIQIVKRHGRRISTALF
                     NLLAYKMDDAPSRVGIIVGKRFGNAVRRNRAKRIFRELVRQLHQDLVPGRGLLVFPKR
                     DVLLQSRDELFQAWRSSLERLNLLRSRAY"
BASE COUNT          656 a          808 c          781 g          783 t
ORIGIN      
        1 cgctgaagtt gcaagaaaga ttcggcccat cagtctacgc tcagatttcc tctctacgct
       61 gggcgagttc ggataaggcc agagccactg cattctctct cgtcaaggca ccgacgatcg
      121 tgaccggtgt cccgacggaa accccctcgt agagaacagc catattcgaa ttatcgagca
      181 tgatgcaccc aagggtctgg gccaaccgtt cattgtcggc tccatggatc tcgatttggc
      241 ccccgatgcc tttggccggg ccgatctttc ccgacctctt cgcaagttcg aatcgtcgac
      301 gatcttcggc attgggataa tccagagcca gggctcggta gaattgcgtc tgccctagac
      361 cgcgcttgtc cgtcacgtga tatcgacctt ccggtgtcgc tccgtctccc tggaactgtt
      421 tttccagcat gccgttgaac cccaaccgga ctgaatatga tagcaccttc cgtccgccct
      481 tgtagagagt caattctcgg tcggccttgc tcaccacgat ggccgttgat tggtggattc
      541 gcgaccaatc gatagcctgt ttggccaaag cctgccaata ggcgatccgg ctctcatcgg
      601 cgtatcgtcc caattctcgg ctgagtaata aagcttgagc cttgagggca tgatctgctt
      661 tctctgccgc ttgcatggat cgctggaagt ctttttgttc gaagaaggaa cgggcctgtt
      721 taacaaggag atccgtttga acgacttttt ctcctaggag aatacgccca tcgatcgatt
      781 cgaccctcga ggtaatcgca tgaagctgct gttcaaggcg aaccaccttg gcctgggccg
      841 cgcgatgctg tgaagtttgt ctctcgtgca gttgggaaac ggtgcgttcg cccatgatgt
      901 atagctgcct gagatcattc tcaagatcgc tcgattccca tggccacctg atcaaatcat
      961 catccaattg gatgtgtgct ttcagagaga tccattgacg aacgaactgc ccgtattcct
     1021 caggcgcagc ttcaggagcc cgcaatgaga tcaaatcctg atcaatggcc tcaagggcca
     1081 caaggaggtc cggcggaacg gcttgaacgc aactggtcaa acagataatg cagagaatcg
     1141 cggcaagcgt cttatgtatc cggttaagcg ccatgccgta cgttactttt tctttgcggg
     1201 tttccctttt cctaccttcg cgagggcgtt ctggatttcg gtggacacgc cttgactcat
     1261 gtcgtgaata gccttcgcct gggtttgcgc ggccgggtaa tcttctttgt cgatcgaggc
     1321 ctgaacctgt ttcagtaacg acttcaaccc ttccacgtcg ttcttgatgg cctccaccgc
     1381 cgcacggtct tttccgaccg gagctttggc gaccaaatct tgcgtcgctt tcaccgcctc
     1441 ttcagcgact tgttgagctt gcaaagctgc ggccttggct tcctcctttt tttgaaccga
     1501 agccgccttg atgcgttcac tatcggcttt ggctgaggcg gatagttgtt gggtcttgcc
     1561 gtagtctcga aagagcgtga acttttcatc ctggtccgcg acttctttcc gcaatgcggc
     1621 aagggtacct tctagcttgg catattcatc agcgctatat gtcgcagcgc cgctttcctg
     1681 tgcatctttg agagcctttt ccgcttcctg gatttgctga gtgggaggct ccgcacatcc
     1741 ggcgattgca acgaaagtca aggcaagaga gacaagggat gcttttctca gcatacaaaa
     1801 aatcctcctt acaggttcta tccacagact ttggtcaatc aagttcttcc ttcatcttag
     1861 agtagtatcc atgatgagta cccagcatta ttcttcaaga agccattagg accattcaag
     1921 atggctgcgc cagcttaaca gacttagatc gtcaggccta ctagcaccct gaaacatgca
     1981 gcaagcccgg tgccacctat aatcgtcgct gtcgaataag gattcaattt atttcgcata
     2041 tttatgccga gtttgatgat ctttggcacc tgattttgtg gcattctggt ggtcgaaagc
     2101 aaaagcgcct caaccctacg gacattacga ctgaagtcgg gcgttcctat taaggggtct
     2161 gatggcagag gcagggtgaa attgacaggt cagagtgcct cccctataat tccccgttca
     2221 tttaaactga ctattcaaag cgtgggaatg gaagggggat tatgtctttc acatttcaac
     2281 aaccgtccaa cttgaaaaag aagcgtgagc acggattcag gaagcgtatg agcaccaaga
     2341 acggccgcaa ggtgctggcc cgccggaggg ctaagggacg agctcgattg actgtctaga
     2401 cacatcgtgt cggatcagca ggtgttcttc tggtcgctct cgatctctcg tgttgctgtt
     2461 gagcgtgcgc tcaccccacc agctagagtc tttccgggat catgaacgga tgggatgcgg
     2521 cgaagaaaca ccctttcaaa tgacaccgaa agcgcaagga cgcaatgtca ttttcttgcg
     2581 ccgcaacagg gacattcaga tcgtcaagcg ccatggtcgc cgaatttcga ccgccctctt
     2641 caatcttttg gcgtacaaga tggatgatgc cccgagtcgc gtggggatta tcgtgggaaa
     2701 gcgatttgga aatgcggtcc gacgcaatcg ggccaagcgg atctttcgtg aactggtgag
     2761 gcaattacat caggacttgg ttccgggccg cggacttctc gtgtttccca aaagggacgt
     2821 attattgcaa tcgcgggatg agttgttcca agcatggagg agctcgcttg agcgcctgaa
     2881 ccttctgcga tcaagagcat attgacatgc aatcgctgtg tctgtggctg attcaaggat
     2941 atcgcctggt aatttcccct ttgtacggtc gcacgtgccg attcgaaccc aggtgttcgc
     3001 aatatgcctc ggatgcaatc actaaata
//
LOCUS       DASTWE010000105         1193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_907632, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000105 DASTWE010000000
VERSION     DASTWE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907632"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..>1193
                     /locus_tag="VFT30_01170"
     CDS             136..>1193
                     /locus_tag="VFT30_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU5091262.1"
                     /translation="MSGLEQFKRLMSYVKPYRIRFVGAFVCSGLVALLSGGYAWLARP
                     VLDDIFIQKDERMLLVLPLALLGIATLKAVFSYGVGYLMAYVGNGVVADIRQQLFQQL
                     MRLSIGFHDTNTSGRLVSRVVNDVGMMANAASSVVKDIFQNGLTFLAMVGVIIYQNWR
                     LAAISLVVIPLSALTMMRVGKRLKRLATSGQEQMGDMASTLQETFASIRVVKAFGREE
                     AEAERFKERNQTFLSTTLKSNQVWSLGHSHMEIIGVIGVATIIWYGGYLVIHEEMTPG
                     AFFSFMAAMFMAYTPIKKLSGANNLIQQALAAAERVFEVLDLETEQSQNRGTVPLAGI
                     NRAIEFQGVSLRYENQTAP"
BASE COUNT          253 a          276 c          379 g          285 t
ORIGIN      
        1 attgtatgaa gaggctcttc ggatcttgga cgatcgaccg gcgtatgatg agatgaaacg
       61 ggatttggca aaggttcggg ctgccttggg tgaaccgggc gcgtcggcca gagccgccga
      121 ggtggtgttg acggcatgtc aggcttagag caattcaaac ggttgatgag ttatgtgaag
      181 ccgtaccgta tacggttcgt aggagctttt gtctgctcag gtctggtggc actgctcagc
      241 gggggctatg cctggctcgc gcgccctgtt ttggacgata ttttcatcca aaaagacgaa
      301 cggatgttgt tggtgctgcc gctggcgctc ttagggatcg cgacgctcaa ggcggtgttc
      361 agctacggcg tgggatattt gatggcttat gtgggcaatg gtgtcgtggc ggacatacga
      421 cagcaattgt tccaacagct tatgcggctg tctatcggat ttcatgacac caatacatcg
      481 ggacgcctgg tctcgcgggt cgtgaacgat gtggggatga tggcaaatgc cgcatcgagt
      541 gtcgtcaaag acattttcca gaacggtctc acctttctcg cgatggtcgg cgtcattatc
      601 tatcagaatt ggagattggc ggcgatctct ctggtcgtga tcccgctctc ggcgctgaca
      661 atgatgcggg tgggaaaacg attgaagcga ttggcgacca gcggacagga gcaaatgggg
      721 gacatggcct caacgctcca agaaacgttc gcgagcatca gggtcgtgaa agcgttcggg
      781 cgggaggagg cggaggctga acgattcaag gaacgaaacc agacctttct ctcgaccacg
      841 ttgaagtcca atcaagtctg gtcgctcggc cactcgcaca tggaaatcat cggagtgatc
      901 ggtgtcgcga ccattatttg gtatggaggc tatttggtca ttcatgagga gatgactccc
      961 ggcgcttttt tttcgttcat ggcggcgatg tttatggcct ataccccgat caaaaaacta
     1021 tcgggcgcca acaatttgat tcaacaggcg ctggcggcgg ctgagcgggt cttcgaggtg
     1081 ttggacttgg aaaccgaaca gtctcagaat cgcggtacgg ttccgctggc gggaatcaat
     1141 cgggccattg aatttcaggg ggtctccctg cggtatgaga atcagacggc ccc
//
LOCUS       DASTWE010000106         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_681309, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000106 DASTWE010000000
VERSION     DASTWE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681309"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..412)
                     /locus_tag="VFT30_01175"
     CDS             complement(5..412)
                     /locus_tag="VFT30_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091263.1"
                     /translation="MTNSIGNPAIPPWLYKLFTGHQYPYVRRLAKFGQTVKPGEDRPE
                     PTKEMIEAKFWEVYLRCRVKVLQEVKEGMIVVFHDLGEYPPGSFQELVDNPEEFLAKT
                     FGKKKIKVNFYDGENFVCTINFKVAGWTEHEHA"
     gene            complement(425..>1402)
                     /locus_tag="VFT30_01180"
     CDS             complement(425..>1402)
                     /locus_tag="VFT30_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5091264.1"
                     /translation="EGRSLPSIVEECLALEMETQPSREFTTHEGRDTFYAEWDYRIED
                     YRMNWCRVVERAADPGSDDFVTKTVVSHQSTVKSLRRFFEGLRPPAFRRVAGQYDGEE
                     VDLDAVVRRTVEQWAGMEGDDRLYIRREKRERDVAVAFLIDISGSTSRDLGTGRQVID
                     IEKEGLVLLCEALDAVGDQYGLYAYSGQGRARIEFLTIKDFDDRLGATTAHRLGGLAP
                     RHQNRDGAAIRHATAKLLAREAKNRVLVLLSDGRPLDDQYKDDYSLEDTKAALREARQ
                     RGVETFCITIDREAENYLRRMYADAQYCVIDRVEALPTKLPGIYRRLTA"
BASE COUNT          288 a          424 c          341 g          349 t
ORIGIN      
        1 gccatcacgc atgttcgtgc tccgtccagc ccgcaacttt gaagttgatt gtgcagacga
       61 aattttctcc gtcatagaaa ttgaccttga tctttttctt gccgaatgtt ttcgccagaa
      121 actcctccgg attgtcgacg agttcctgaa agctaccggg aggatactca ccgaggtcat
      181 gaaagaccac gatcatccct tctttgactt cctgcagcac tttcacccgg caacgaagat
      241 agacttccca gaactttgct tcaatcattt ccttcgtcgg ctccggacgg tcttcaccgg
      301 gtttcaccgt ctgtccgaac ttggctaatc gacggacgta gggatactga tgccctgtga
      361 agagcttgta caaccatgga ggaatcgccg gatttccgat tgagttcgtc atgttcttga
      421 gttcttacgc cgtcaatcgt ctgtaaattc caggcagctt cgtcggaagg gcttcgacgc
      481 ggtctatgac gcagtactgg gcatcggcat acatgcgacg tagataattc tctgcttccc
      541 gatcgattgt gatacaaaag gtctcgactc cccgttgtct agcctcgcgg agcgccgctt
      601 tcgtatcttc caacgagtag tcgtccttgt actggtcgtc caatggtctt ccgtcgctca
      661 acagtacgag gacacggttc ttggcttccc gcgccaacag tttcgccgtc gcatgacgaa
      721 tagctgcacc atcgcgattc tgatgacgag gagccaagcc accaagccga tgggccgtcg
      781 tcgctccaag gcgatcgtca aagtctttga tcgtgagaaa ctcgatcctc gctcgtcctt
      841 ggcccgagta ggcatagagt ccatactgat cgccgacggc gtccagtgct tcacagagca
      901 gcaccaagcc ttctttctca atgtcgatca cctgtcggcc ggttccgagg tcacggcttg
      961 tggatccact gatatcgatg aggaaggcaa ccgccacgtc gcgttctcgt ttttctcgcc
     1021 ggatgtacag ccgatcgtcg ccttccatgc ccgcccactg ttccactgtt cgtcggacaa
     1081 cggcatcgag atccacctct tctccgtcgt attgtcccgc aacgcggcgg aacgccggtg
     1141 gacgcaaacc ttcaaaaaac cggcgcagtg acttgacggt actctggtgg gagaccaccg
     1201 tcttcgtgac gaaatcgtcg gatcctgggt ccgccgctcg ttctaccaca cgacaccaat
     1261 tcatccggta atcctcaatc cgatagtccc actcagcata aaaggtgtct cgcccttcat
     1321 gagtcgtgaa ctcccggctc ggttgtgttt ccatctcaag tgcgaggcac tcttccacaa
     1381 tggacggcaa tgaacgcccc tc
//
LOCUS       DASTWE010000107         1480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_519896, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000107 DASTWE010000000
VERSION     DASTWE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519896"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFT30_01185"
     CDS             <1..246
                     /locus_tag="VFT30_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEU5091265.1"
                     /translation="KHIIYRKKKGFDNPLAAWFRTSMRSFVEEALFSKDSATSMYFDQ
                     QYICWMLEQDRQGTEQLRRHIHLLVSFELWHREFIGV"
     gene            249..1223
                     /locus_tag="VFT30_01190"
     CDS             249..1223
                     /locus_tag="VFT30_01190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5091266.1"
                     /translation="MGTESPIELSIIIVNWNSRDFLQKCIASILDAKIKCQHEIIVID
                     SGSFDGSREMVKQCYQPVKFIQSEINLGFAKANNVAFKASSGSQILFLNPDTKVVAPA
                     IDILLDQIMKLPKAGAIGCKLLNEDGSVQTSCIQSFPTILNQFLNSELLMSLFPKSKL
                     WGMASLFGSANGPAEVEMLSGACLMMKRTLFEQVGYFSEEYFMYAEDVDLCYKVRQAG
                     YRNYYVPNATVFHFGGGSTQQRPNDFAVIMMRASVLKFLRKTRGELYGFWYRLSMLIL
                     AMGRILCLLVLSPLYLSLGWLASWKASARKWWVISAWSMGLKQVGEKS"
BASE COUNT          373 a          333 c          403 g          371 t
ORIGIN      
        1 aaacacatta tctatcgtaa aaagaaggga ttcgacaatc ccctggcagc gtggttcagg
       61 actagtatgc ggtcttttgt ggaagaggcg ctttttagca aggattccgc taccagcatg
      121 tatttcgatc agcaatatat atgctggatg ttagaacagg accgccaagg gacggagcag
      181 ctacgccgcc atatccatct gttggtgtcg ttcgaattgt ggcaccggga gtttataggg
      241 gtttgaagat gggaaccgaa tcgccgatag aactgtccat aattatcgtc aattggaatt
      301 ccagagattt tcttcaaaag tgtattgcct cgatcctgga tgcgaagatc aagtgccagc
      361 acgaaatcat cgtgatcgac agcggatcgt tcgatggctc ccgcgagatg gtgaaacaat
      421 gttatcagcc ggtgaagttc atccagagcg agataaatct cggctttgcg aaggcaaaca
      481 atgtagcctt taaagcatca agcggcagcc agatactatt cttaaatccg gataccaagg
      541 tggtcgctcc ggccatcgac atccttcttg accagattat gaagttgccg aaggcaggcg
      601 caatcggatg caagttgctc aatgaggatg gatcggtaca aaccagctgc attcaatcgt
      661 tcccaactat cttgaatcag ttcctgaact cagaattgtt aatgagccta ttcccaaagt
      721 ccaagctctg gggaatggca tcacttttcg gttccgcgaa tggcccggcg gaagtggaaa
      781 tgctttctgg ggcttgtctc atgatgaaac ggacgctctt cgagcaagtc ggctatttta
      841 gcgaggagta tttcatgtac gccgaggatg tggatctctg ctacaaagtc aggcaagccg
      901 gttataggaa ttattacgtc ccgaacgcca cggttttcca ctttggcgga ggaagcacgc
      961 aacagaggcc aaatgacttc gctgtgatca tgatgcgggc gtccgtgttg aaattcctca
     1021 ggaagactcg aggagaactg tacggattct ggtatcgatt gtcgatgctg attttggcga
     1081 tgggacgcat cctctgtttg ctggttcttt cgcctctcta tcttagcctg gggtggctcg
     1141 cgtcctggaa agcttcggcg agaaagtggt gggttatttc agcgtggagt atgggactga
     1201 agcaggttgg agagaaatca tgaagaaaac aatcaattag ttcggaagag aaccgattac
     1261 cctagaccat tctgccctca tctaatcatc gtgtgtggct ttgtcggaaa acttaatcta
     1321 gacagaaatc agccggttcc acgtgagctc atccggcaga tgatgacgga aatcgttcat
     1381 cggggccctg atggggaagg tgattacctc tccggcccgg ttgggcttgg ccacagacgg
     1441 ctctccatca tcgggataga gagcggcgct cagccgatga
//
LOCUS       DASTWE010000108         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1295398, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000108 DASTWE010000000
VERSION     DASTWE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295398"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1181)
                     /locus_tag="VFT30_01195"
     CDS             complement(27..1181)
                     /locus_tag="VFT30_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091267.1"
                     /translation="MALIVASDVASRSEAAEWSLSPSVGVKGVYNSNLLLTPLPHDET
                     YGYWVTPAAEVAGKTERLEVSSRVAADVVGYFGGEDKQFTNAFAPLSVRYKTEKDLLG
                     FTGGFTRDNTLLSELQETGVVLQFAQRNQWAFNPTWTRSLTEKLSFQSGVQFSDTTYE
                     SARLVDYRVMGGSGGLRYQLTERDQIQVLGSYIHFRTTDSPFGFQADFPGINMSLTHA
                     FDESLTGTVYGGPRFLSSTSQAQVGDITARDTVWLAGANMTKNFERATLQVSFARDLV
                     PSGFGLLIQTNRGELSGSYEISETLACSLNVVGVLTSGKTPAAIGGVFPDSSYVSLTP
                     KLSWKFLEWWQAEVSYMYRWRDIDSVADSAQSHATMFTVTYYPPKLSLSY"
     gene            complement(1259..>1580)
                     /locus_tag="VFT30_01200"
     CDS             complement(1259..>1580)
                     /locus_tag="VFT30_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEU5091268.1"
                     /translation="LVKRLTKYIPNPNVSVAVTKVVSYKVYVVGRVNKPGEYLIGHYT
                     DVLQALSLAGGLTPFAAENDIKVMRRVRGEQYAIPFRYGDLRKGRDLEQNIILQRGDV
                     VMVP"
BASE COUNT          371 a          453 c          401 g          355 t
ORIGIN      
        1 agtgagacgt gtgtttgtgc cataactcag taggacaatg aaagtttcgg tggataatag
       61 gtcaccgtga acatcgtcgc atgggattgg gcggaatctg caacggagtc aatgtcacgc
      121 catcggtaca tgtacgatac ttccgcctgc caccattcga gaaacttcca cgataacttc
      181 ggcgtcaggc tgacatagct gctatccgga aagactccgc cgatggccgc tggtgtcttt
      241 cccgacgtaa ggacgccgac cacgttgagg gaacaggcca gagtttccga gatttcgtat
      301 gacccggata gttctcctcg attggtttgg atgagcaatc caaatccact gggcacaaga
      361 tcccgagcaa aggatacttg cagtgtcgcc ctctcaaaat tcttcgtcat attcgctcct
      421 gccaaccaga ccgtatcgcg agccgtgatg tcacccacct gagcctgtga ggtagaagac
      481 aagaatctgg ggccaccata gacggtccct gtcagcgatt cgtcgaaggc atgcgtcagg
      541 ctcatgttga tcccgggaaa atccgcttga aacccaaatg gtgaatcggt cgtgcgaaag
      601 tgaatatacg accccagcac ttggatctga tcacgctccg tcagttgata gcggagtcca
      661 cctgaacctc ccatcactcg ataatcaacc aacctggcgc tctcatacgt ggtgtctgaa
      721 aattgtacgc ccgattgaaa tgataacttt tcagtcaaac ttcttgtcca tgtcgggtta
      781 aatgcccact gatttcgctg cgcaaactgc aggacgacgc cggtctcctg taactcactg
      841 agcagcgtat tgtcacgagt gaagccaccg gtgaagccga ggagatcctt ctcggtctta
      901 tatcgtacgg acaacggagc aaaggcattc gtgaactgtt tgtcctctcc accgaaatac
      961 ccgacaacat ccgcggcgac ccggctgctg acctcaagcc gttcggtctt gccggcaacc
     1021 tctgccgctg gagtaaccca atagccgtag gtctcgtcgt gaggcagagg ggtcaagagc
     1081 aagttgctgt tgtaaacccc tttgacgccg acggatggtg agagtgacca ctcagcggcc
     1141 tcgcttcgtg acgcgacgtc agatgcaacg atcagcgcca cgaaaagcac gtggacggcc
     1201 tgcctccgac tcctaaccca gtctctccgc ttgccgcttg agtacatgag ggcgacggtc
     1261 agggcaccat gacaacgtca ccgcgctgga gaatgatgtt ctgctccaag tctctccctt
     1321 tccgaagatc tccatagcga aagggaatgg catattgttc tcccctcact cgccgcatga
     1381 ccttgatatc gttttccgca gcaaacggag tcagtccacc ggcgaggctg agcgcttgca
     1441 gcacgtcggt gtaatggccg atcaagtact cacccggctt attcactcgc ccgaccacat
     1501 acaccttgta actgacgact ttcgtgaccg ccacggacac gtttggattt ggaatatact
     1561 ttgtcaaccg tttcaccaga
//
LOCUS       DASTWE010000109         1199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1101622, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000109 DASTWE010000000
VERSION     DASTWE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101622"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..520
                     /locus_tag="VFT30_01205"
     CDS             131..520
                     /locus_tag="VFT30_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091269.1"
                     /translation="MPKAMLAKGDETILLVKENEIERKLALSALQRYRYRVLEAGSSV
                     EALMLTQQYNGIVHLTVSPLVMPEIWGRELARRLLRQHPTMKTLFVSGYDDETIQHHR
                     INQRFVLQQPYRQSGLIEKVRDVLDAA"
     gene            complement(551..>1199)
                     /locus_tag="VFT30_01210"
     CDS             complement(551..>1199)
                     /locus_tag="VFT30_01210"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5091270.1"
                     /translation="IFIGICIAVFLYQVNLPQEAAELFAFQYGAIPAIVFGQASLPEE
                     AVAIPVSLTLVTSMFLHGGWMHLLGNMLYLWIFGNNIEDAMGHTKFVVFYILSGILAA
                     LSHALTDPSSQIPMVGASGAISAVLGAYLLLFPRAYVLVLLPAIGMTRVAAGVVLGMW
                     FITQLVSGGMSIGATGGGVAFFAHIGGFIAGMALIGLFKRKEVRFFAPGRSTWSS"
BASE COUNT          311 a          316 c          340 g          232 t
ORIGIN      
        1 gtctcaccgc cgtcttcggg atcgtcaaac agaacggagg aacgctggag gttgatagtc
       61 gaccaggaca agggaccgtc gtccaaatcg tcttccctgc ggtccatcgc cggagacatg
      121 gaaagaagtg atgccgaaag ccatgttggc caagggagat gaaacgattc tgcttgtgaa
      181 ggaaaacgag atcgaacgga agctggcatt gtcggcttta cagcggtatc gctatcgagt
      241 cttggaggcg ggctcatcgg tcgaggcgct tatgctcacc cagcaataca atggaattgt
      301 tcatctcacc gtgagtccct tggtaatgcc agaaatctgg ggacgtgaac ttgcccgccg
      361 actcttgagg cagcatccga cgatgaagac actgtttgtc tcaggttacg atgatgaaac
      421 gattcaacat catcggatta accagcggtt tgtcctgcag caaccctacc gtcagtcagg
      481 attgatcgaa aaggtcagag acgtgttgga tgctgcctga gcatcctttc cggttctggg
      541 aggcacgtcc tcatgaagac cacgtcgacc ggcccggcgc aaaaaatcgc acttctttcc
      601 gtttgaagag accgatcagt gccatgcccg cgatgaatcc accaatatga gcgaaaaagg
      661 cgacgccacc tccagtggct ccgatgctca tgcctccact gaccagctgc gtgataaacc
      721 acatgccgag gacgacgccg gccgctactc gggtcatccc gatcgctggc agcaagacca
      781 gcacgtaagc gcgtggaaat aagagcagat aggcaccgag cacggccgag atggccccgc
      841 tcgccccaac catcgggatt tgggaggaag gatcggttaa tgcatggctc agcgcggcga
      901 ggataccgga taggatgtag aagacgacga atttcgtgtg acccatcgca tcctctatat
      961 tattcccgaa aatccagaga tagagcatgt tcccgagtag atgcatccag cctccgtgaa
     1021 gaaacatgct ggttaccagg gtgagggaca ccggaattgc aacggcttcc tcaggaagcg
     1081 aagcctggcc gaagacaatg gcagggatgg caccgtattg aaacgcgaag agctcggctg
     1141 cctcctgagg aagattcact tgatagagaa acacagcgat acagatgccg atgaagatc
//
LOCUS       DASTWE010000110         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1327778, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000110 DASTWE010000000
VERSION     DASTWE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327778"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFT30_01215"
     CDS             <1..499
                     /locus_tag="VFT30_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091271.1"
                     /translation="TLSTITPIADKNRHRLITVVDPSLPPVAGDEGRLVQVLTNLLDN
                     AIKYTPAGGTITIGGKLASSVGNAEPPARAIEISVADTGIGIPEEDRPRVFERFYRVD
                     KARSRELGGTGLGLAIVKHIVEGHGGQVWVEANYPQGSRFVVRLPLSAAARGGLHHMN
                     EASRA"
     gene            complement(472..1464)
                     /locus_tag="VFT30_01220"
     CDS             complement(472..1464)
                     /locus_tag="VFT30_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU5091272.1"
                     /translation="MTELSGILLVTVVLALLFDFSNGWHDCANAVATVVSTRVLSPVA
                     AVLLAGVLNVAGAFFSTAVAKMIGGGVVFPDAITNTVVAGAMGGAILWNFFTLMLGLP
                     TSSSHALIGGLVGSAVAHGGWSVVQFNGLRKILEAMVLSPLLGFGIGFLLMVLVSWVF
                     FRVHRGVATKVFSRLQIVSASFMAFSHGANDAQKVMGVITLALVTSGQLTSSEVPTWV
                     IVACALAMGLGTMVGGWRIIRTLGIKMVKLEPVHGFSAETAAASVLLFTAHFGLPVST
                     THTITSAILGVGSTKRLSAVRWGVTSKIFSAWVFTLPGAALLGAGVYALLASFM"
     gene            complement(1561..1716)
                     /locus_tag="VFT30_01225"
     CDS             complement(1561..1716)
                     /locus_tag="VFT30_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091273.1"
                     /translation="MSRDAISELFEKETNPIAVIKWKEIYETFEEGTDRCEDVANVIE
                     CIVLKQT"
BASE COUNT          414 a          526 c          474 g          318 t
ORIGIN      
        1 gacgctgtca acgatcactc cgatcgctga taaaaatcgt caccggctca ttacagtggt
       61 cgatccatcg ctccctccgg tggcgggcga tgaaggccgg ctcgtgcaag tcttgaccaa
      121 tctgctcgat aacgccatca aatatacgcc ggcggggggg accatcacga tcggcggcaa
      181 gcttgcttcc tcggtcggaa atgccgagcc accggcaaga gcgatcgaga tcagcgtcgc
      241 cgataccgga atcggtattc ccgaagagga ccggcctcgc gtcttcgaac ggttttaccg
      301 cgtcgacaaa gcccgctcac gagagttggg agggacggga ctcggactgg cgatcgtgaa
      361 gcatatcgtt gaggggcatg gaggacaggt gtgggtcgaa gcgaattatc ctcaagggag
      421 tcgattcgtg gtccgtcttc cgctcagtgc tgcagcccgt ggtggcctcc atcacatgaa
      481 cgaagcaagc agggcataga cacccgctcc caacagtgcg gcgccgggca atgtaaacac
      541 ccaagccgag aagattttac tcgtcactcc ccatcgaacc gcggacaggc gcttggtcga
      601 tccaacacct aagatcgcgg aagtgatggt gtgggtcgtg ctgaccggaa ggccgaagtg
      661 cgcggtgaac agtaaaaccg aagccgctgc cgtctcagcc gagaatccgt gaaccggctc
      721 gagtttcacc atcttgatcc ctagagtccg aatgatccgc cacccgccca ccatggttcc
      781 gagccccatc gccagcgcac aggccacgat gacccacgtc ggcacttccg atgatgtcaa
      841 ctgaccggac gttacgagag caagtgtaat gacacccatc accttctgag catcgtttgc
      901 cccatggctg aatgccatga agctggccga aacgatttgg agtcgactga atactttggt
      961 cgccacaccg cgatgaactc gaaaaaacac ccagctgacc aggaccatca ggagaaatcc
     1021 tatcccgaat cccaagaggg gggagagaac catggcttcg agaatcttcc gcaggccgtt
     1081 gaactgaact accgaccagc cgccgtgcgc caccgccgaa ccgacgagtc caccgatcag
     1141 cgcgtgagag gaactggtcg gcagtcccag catcagcgtg aaaaaattcc agagaattgc
     1201 gccgcccatc gctccggcaa ccaccgtatt cgtgatcgcg tcggggaaga cgacacctcc
     1261 tccgatcatt tttgccaccg ccgtcgaaaa gaaggccccg gcgacattca acaccccggc
     1321 caacagcacc gcggcgacgg ggcttaacac ccgggtggaa acgaccgtcg ccacagcatt
     1381 ggcgcaatca tgccacccat tcgaaaagtc gaacaggagc gccaacacca ccgtcacaag
     1441 aagtatgccg ctgagttcag tcatggttcg attgaaatac tatccgccca tccgacgacc
     1501 attcaggcgt ggagggcttg aggagatata gatgccgggc ggaggtccat tcatgggcga
     1561 ttaggtctgc ttgagcacaa tacattcgat gacgttggcg acatcttcac accgatcggt
     1621 cccttcctcg aaggtttcat aaatctcttt ccacttgatg acggcgatcg ggttcgtctc
     1681 tttctcaaac aactctgaaa tggcatcgcg tgacacacga tcggcctcgt tc
//
LOCUS       DASTWE010000111         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1327784, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000111 DASTWE010000000
VERSION     DASTWE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327784"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFT30_01230"
     CDS             complement(<1..321)
                     /locus_tag="VFT30_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEU5091274.1"
                     /translation="MTASASTSPKIDRPLNVIVATDCGSTTTKAILIEKIGDEYRQTY
                     RGEAPTTVEAPFEDVTRGVLNAIAEIEELSGRKILDGDNIITPCRDDKTGVDIYISTS
                     SAGGG"
     gene            complement(318..689)
                     /locus_tag="VFT30_01235"
     CDS             complement(318..689)
                     /locus_tag="VFT30_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091275.1"
                     /translation="MRSLFKGNAAEKLAKVRAGLRHAFAVQAETQPLVIEDVQLLERI
                     AETIVKRGMAAPATMFLESMGPMNFLGSQALHFITPIIECAFNAKEVEQIARLLERRD
                     TVSRLIAIIEAKSAPKGPTAQ"
     gene            900..>1183
                     /locus_tag="VFT30_01240"
     CDS             900..>1183
                     /locus_tag="VFT30_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091276.1"
                     /translation="MRKRRSSYEEQIARDYEKGRFKSVVPSKADIKRFKEAARATFIK
                     NRRVNVRLSSPDLMDLQTRAAEEGVPYQTLIASVLHKFVTGRFTEKPSRLT"
BASE COUNT          283 a          298 c          320 g          282 t
ORIGIN      
        1 cccgccgccg gcgctgctgg tcgagatata aatatcgacc cccgtcttgt catcccggca
       61 gggcgtgatg atgttgtcac catctaaaat cttccgaccc gagagctcct cgatttcggc
      121 aatggcattg agcacgccgc gcgtcacatc ctcaaatggg gcctcgaccg tcgtcggcgc
      181 ttcgccgcga taggtctgcc tatactcgtc accgattttc tcaatgagaa tggctttggt
      241 ggtggtgctc ccacaatccg tcgcgacgat cacgttgaga ggacgatcga ttttgggaga
      301 ggtagaagcg gaagccgtca ttgagccgtt ggtccctttg gagcagactt ggcttcaatg
      361 atggcaataa ggcgagacac cgtgtcacgg cgctcgagca atcgcgcgat ctgttcaact
      421 tctttggcgt tgaacgcgca ttcgatgatt ggtgtgatga agtgcagtgc ctgacttccc
      481 agaaaattca tgggtcccat ggattccaaa aacatcgtcg ccggagccgc catgccgcgt
      541 ttgacgattg tctcggcaat ccgctccagt agttggacat cttcaatgac gagaggttga
      601 gtctcagcct gaacggcaaa tgcatggcgc agtccggctc gaacttttgc cagtttttct
      661 gctgcgtttc ccttgaagag cgatcgcata agtgtaggcc agataaggcg aacccagaac
      721 ggatcgcatt atatgaaggg ggtgaaggag agtcaattca atggtggaac cgcgaacctg
      781 gtttcaatgt atagtatgcc tacttggttc cgttcgtgga agaagttgac cactacttcc
      841 ttaaaacgat cattccgaac aggaaggcta cccgtgacta cttgaaaggc ggtgtgtaaa
      901 tgagaaaacg tcgatcatcg tatgaagaac aaattgccag agactatgag aagggtcggt
      961 ttaagtccgt tgttccctca aaggcggata tcaagcgctt caaagaagcg gctcgcgcga
     1021 cttttatcaa gaaccggagg gtcaatgtga gattgtcctc acccgactta atggatctcc
     1081 aaacccgtgc tgctgaagaa ggggtgcctt accaaacgct cattgccagt gtgctacata
     1141 aattcgtgac aggtcgattc acagagaagc catcgcgtct cac
//
LOCUS       DASTWE010000112         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_132164, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000112 DASTWE010000000
VERSION     DASTWE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132164"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1335
                     /gene="pheA"
                     /locus_tag="VFT30_01245"
     CDS             259..1335
                     /gene="pheA"
                     /locus_tag="VFT30_01245"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU5091277.1"
                     /translation="MPKDMSEYRKEIDRIDDEIIRLLNERSKSVVEIGKLKKEKDADA
                     NLHTAGREAEIVERLTKLNTGPFPSEAIQSVYREIMSASLSLEAPQKVAYLGPRATFT
                     HMACMQKFGSSVQYVPVHSIKEVFSEVERSRANFGVVPIENTTEGVVNHTLDMFIDSN
                     LLIYGEVLQEVSHHLLSKSGLIEDVKKIYSHPHALAQCRNWLETNLPHVPSIEVASTA
                     RAAELCIDEPASAAIASELAGQLYGLKVMKARIEDNLNNFTRFLVLSHKPSERTGKDK
                     TSLMLSVKDKVGALYDLLRPFASHGINMTKIESRPSQRKAWEYIFFVDVEGHINEDRV
                     NKAVEEVKSRCLFMKILGSYPIHS"
     gene            1338..>1583
                     /locus_tag="VFT30_01250"
     CDS             1338..>1583
                     /locus_tag="VFT30_01250"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU5091278.1"
                     /translation="MALQVHPDIRSLSPYVPGKPIDELQRELGLTRVIKLASNENPLG
                     PSPRALAALSGAQDMLHRYPDGGGYQLRQTIADRWKVG"
BASE COUNT          396 a          411 c          407 g          369 t
ORIGIN      
        1 tttgatccaa gccggcttcc tggatcgtac cggtcgtggc cggcaagtaa ctcgtttggc
       61 gtacgatcac tttcaacggc cttctccctt gctgatgtaa aacagtagat tccgattttt
      121 caaaactcct gatatccaag cgctatcctt gcaatagatt caatcgttcc tgtaagattc
      181 cctacttgtc tgtaatcccc catcctacgg ggatgaggaa gtcctccccg caatcgagtg
      241 cccgaggcga gactcagcat gcccaaagat atgtcagaat atcgtaaaga aatcgatagg
      301 atcgatgatg aaatcattcg gctgctcaat gagcgctcga aaagcgtcgt cgagatcgga
      361 aaactcaaga aagaaaagga tgccgatgcc aacctccata ccgcgggtcg ggaggcggag
      421 atcgtcgaac ggctcacgaa actcaacaca ggacctttcc ccagtgaggc tatacaatcg
      481 gtttatcgag aaatcatgtc ggcttccttg tcgctcgagg ctccccaaaa ggtagcgtac
      541 ctggggccgc gggcgacctt tacccacatg gcctgcatgc agaaattcgg ttcgtccgtc
      601 cagtatgtgc ccgttcacag tattaaagag gtgttcagcg aagttgagcg aagccgggcc
      661 aattttgggg tggttcccat cgaaaacacg acggagggtg tcgtgaatca caccctcgac
      721 atgtttatcg attccaacct actgatctat ggagaggttc ttcaagaggt ttcgcaccat
      781 ctgctgtcga aatcaggcct tatcgaggat gtgaagaaaa tctactctca tcctcatgcc
      841 cttgcgcaat gccgaaactg gctagagacc aatctcccgc atgtcccgtc gatcgaggta
      901 gccagcactg ctcgggcagc agaactgtgc atcgatgagc cggcctcggc cgcaattgcg
      961 tcagagttgg ccggccaact ctacggactg aaagtgatga aggcccgtat cgaagacaac
     1021 ttgaacaatt tcactcgttt cctggttttg tctcataaac cgtcggagcg gacagggaag
     1081 gataaaactt cattgatgct gtcggtgaag gataaagtcg gagccctcta tgatctgctc
     1141 cgtccgttcg cctcgcacgg aatcaatatg acgaaaatcg aatcccgccc atctcagcgc
     1201 aaagcatggg agtatatatt ttttgtggat gtcgaaggac atatcaatga agaccgggtc
     1261 aacaaggccg ttgaagaggt gaagtcgcgc tgtctcttta tgaagatttt aggctcctac
     1321 ccgattcata gctgatcatg gcactacagg tccaccctga tatccgatct ctgagtccgt
     1381 acgttcctgg taagccgatc gatgaactgc aacgggaact tggactcacc cgcgtgatca
     1441 aacttgcgtc caacgagaat ccactggggc cttcaccgag ggccttggca gcactgagtg
     1501 gtgcgcagga tatgctgcat cgatatccgg atggaggtgg ctatcaacta cgccagacga
     1561 ttgctgatcg ttggaaagtg gga
//
LOCUS       DASTWE010000113         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_907781, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000113 DASTWE010000000
VERSION     DASTWE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907781"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..799)
                     /locus_tag="VFT30_01255"
     CDS             complement(143..799)
                     /locus_tag="VFT30_01255"
                     /inference="COORDINATES: protein motif:HMM:NF022620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HEU5091279.1"
                     /translation="MLRVVLAVMLVFIPLSLSSCDKAYMATMEKMGYAKRDILSSRVK
                     SARDAQEDAKKEIQSTLEQFGKVVSYEGGDLEDTYKKLSGELETSEDSAKSVRKRIAD
                     VESVADALFSEWEEELGQYSSADLRRKSQAKLTQTKGRYREMLAAMKRAEQRIEPVLK
                     PLRDQVLYLKHNLNARALAAIKGELVKVDAQVDQLVKDMNRSIAEADKFIQSMEKDSD
                     "
     gene            965..>1404
                     /locus_tag="VFT30_01260"
     CDS             965..>1404
                     /locus_tag="VFT30_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU5091280.1"
                     /translation="MRLMFSNLWWVGVLVLAVACSSQRVIPEELEPLVDRAIPFHEVA
                     AAPDSYQGKIVVFGGEVLKAKRLKDGTQIELLQLPLDKGERPILDRQQSQGRFLAVQQ
                     EFLDPATIVEGTKMTIVGEVSRAKTEYLDDVEYRYPVLVVKYLHT"
BASE COUNT          277 a          389 c          387 g          351 t
ORIGIN      
        1 ggcgagtgcc agtacgattg tgccaatgcc gaggccgcct cttcctccga ggcgagcggg
       61 acccatgccg cggcgatctt cgatgttgtc gctttcgcgt tgatcttcaa gacgcatagc
      121 cgtgctccat catgccgcac agtcagtccg agtccttctc catcgattga atgaatttgt
      181 ccgcttcggc gatggaacgg ttcatgtctt tgactagctg atcgacctgc gcgtcgactt
      241 tcacgagttc ccccttgatc gcggcgagcg cacgcgcgtt caggttgtgt ttcaggtaga
      301 gcacctgatc gcgcaacggt ttgaggaccg gttcaatccg ctgttcggcc cgtttcatgg
      361 ccgcaagcat ctctcggtaa cggcccttgg tctgggtgag tttggcctga ctctttcggc
      421 gcaggtcggc gcttgagtat tgaccaagtt cttcttccca ttccgagaag agtgcatcgg
      481 ccacgctttc gacgtccgcg atccgtttcc ggaccgattt ggcactgtcc tcgcttgtct
      541 ccagttcacc gctcagcttc ttataggtgt cttccagatc tcccccttca taggagacca
      601 ccttaccgaa ttgctccagc gtgctttgaa tctctttctt cgcatcttct tgcgcatccc
      661 gcgccgattt gacacggctg ctcaagatgt cgcgtttggc atagcccatc ttctccatgg
      721 tggccatata ggccttgtcg caggaagaaa ggcttaaggg tatgaacacc agcatgacgg
      781 ccaagacgac tctcaacatg atcacctctc tacgcggata tggcatcgac gatgagcgcg
      841 tctcccgtgt cccagcgcga ctctgcatca taaccgaaga aagcacagtg ggaaagaccc
      901 taggtcgacg ttctattgga cttgctgtgt agaaacggca tcgtcgtcgc gctatagtgt
      961 aaacatgcgt cttatgtttt ccaacttgtg gtgggtgggg gtgttggttc tggcggtagc
     1021 ttgttcctca caacgagtga ttcctgaaga actggagcct ctagtcgacc gagcgatccc
     1081 gtttcacgaa gttgcggcgg cccctgattc ataccagggc aagatcgtgg tatttggtgg
     1141 ggaggtgctg aaggcaaaac gactgaaaga cgggacacag atcgagcttc tgcagttgcc
     1201 gctcgataag ggcgagagac cgattctcga ccgtcaacaa tcgcaggggc gcttcctcgc
     1261 ggtccagcag gagtttcttg atcccgcgac cattgtcgaa ggaacaaaga tgacgatcgt
     1321 tggagaagtg tccagggcga agaccgaata ccttgacgat gtcgaatatc ggtaccctgt
     1381 gttggtcgtg aaatatctgc acac
//
LOCUS       DASTWE010000114         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1166151, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000114 DASTWE010000000
VERSION     DASTWE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166151"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFT30_01265"
     CDS             <1..365
                     /locus_tag="VFT30_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU5091281.1"
                     /translation="PKAGLVFDTLYRAGEWVEAGRPVIVLLPPDHIKVRMFVSETKVG
                     AIKLGDVVQITVDGVQGTIQGKVSYISPRAEYTPPVIYSQENRKKLVFLVEALFDQDV
                     ARDLHPGQPVDVQFDGAL"
     gene            362..1303
                     /locus_tag="VFT30_01270"
     CDS             362..1303
                     /locus_tag="VFT30_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5091282.1"
                     /translation="MKRDVNGQELAIDVHGMTKRFGSRTVVDNIGLQIRRGEIYGFLG
                     PNGSGKTTFIRMLCGLLRPDEGSGTCLGYDVITESEAIKAAVGYMTQRFSFYEDLSIA
                     ENLDFVARMFGVDRRRQAVEQSLERLGLAGRREQLAGQLSGGWKQRLALAACLIHRPL
                     LLLLDEPTAGVDPKARREFWEQIHGLAAEGLTFLITTHYMDEAERCHRLAYIAYGKLL
                     TYGTVADVIVQAQLTTWSVSGPDLQQLAEILRRQPGVQQAVAFGDRLHVSGDDPVVLE
                     ASVSAFRAPPYEWHRVNTSLEDVFIHLMHRSSNEVTS"
     gene            1300..2442
                     /locus_tag="VFT30_01275"
     CDS             1300..2442
                     /locus_tag="VFT30_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5091283.1"
                     /translation="MSLRRTFSPSRFWAIVVKEFIQMRRDRVTFGMMIGIPLIQLILF
                     GFAINADPKHLPTAVLLADYGPHGRTLLQAIRNSDYFEVTRQVMSEREAEEVLARGDV
                     QFVINIPYNFSRDIVRGEKPAILVEADATDPAATSNAIGSLRVLAATALRRDLTGPLA
                     FLSRNDDPIDLRIHARYNPEAITQYNIVPGLMGVVLTMTMVMITGLAITRERERGTME
                     NLLSMPTRPFEVMIGKVLPYILVGYVQVVLILLAAHLLFKVPVYGNIPMLFLAALIFI
                     TANLAMGITFSTMAQNQLQAVQLSFFFFLPSLLLSGFMFPFRGMPQWAQTIGEVLPLT
                     HFLRIVRGIMLKGNGPEEIVLQLWQIALFAVVALAIGVKRYRQTLD"
BASE COUNT          541 a          716 c          760 g          613 t
ORIGIN      
        1 cgccgaaggc ggggcttgtc ttcgacaccc tctaccgcgc aggggaatgg gtagaggccg
       61 gacgtcccgt gatcgtcttg ctgccgcccg atcacatcaa agtgcgcatg tttgtatccg
      121 agacaaaggt tggggccatc aaactcggcg atgtagttca gattacggtg gatggcgtac
      181 aaggcacgat tcaggggaag gtgagctaca tttcgccgcg ggcagaatat accccgccgg
      241 tcatctacag ccaggagaac cgtaagaaac tggtcttttt ggttgaagcg ctgttcgatc
      301 aggatgttgc acgcgatctg catcctggtc aaccggtaga tgtgcaattc gacggtgctc
      361 tatgaagcga gatgtgaacg gacaagaatt ggccattgac gtgcacggca tgacgaaacg
      421 gtttgggtca cggaccgtgg tcgacaacat cgggctgcag atacgtcgcg gcgagattta
      481 cggctttctc gggccgaatg gcagcggcaa gaccacgttt atccgcatgc tgtgcggtct
      541 tttgcgcccg gatgaaggga gcggtacttg tcttggctac gatgtcatca ccgaaagcga
      601 agccatcaaa gccgctgtcg gctatatgac ccagcgcttt agtttctacg aagacctgag
      661 catcgccgag aatctcgatt tcgtcgcgcg gatgtttgga gtcgatcgtc gccgtcaggc
      721 cgtcgagcag agcctcgaac ggttgggcct cgccggtcga cgagagcagc tggctggtca
      781 gttgtcagga gggtggaagc agcggcttgc cttagcagcc tgtctgatcc atcgaccact
      841 gttattgcta ttggatgagc cgacggccgg tgtcgatccg aaagcgcgca gagaattttg
      901 ggaacagatt cacggattgg ccgccgaagg cctcactttc ttgatcacca cccactatat
      961 ggacgaagcc gagcgctgcc atcgccttgc ctacatcgcc tacgggaagt tactcaccta
     1021 tggaactgtc gcggatgtca ttgtccaggc gcagcttacc acctggtccg tcagcggacc
     1081 ggatctccag caattggcgg aaatcttgcg ccgacaaccc ggcgtgcaac aagccgtcgc
     1141 gtttggcgac cggttgcatg tgagcggaga cgatcctgtc gtactcgaag catcggtttc
     1201 ggcgtttcgt gctccgccgt atgaatggca tcgcgtgaac acgagtctcg aagatgtgtt
     1261 catccatctc atgcatcgat cctcgaatga agtcacatca tgagcctgcg acggacattc
     1321 tccccctccc gcttctgggc catcgtcgtc aaggagttca ttcagatgcg ccgggaccgt
     1381 gtgacgttcg ggatgatgat cggcattccg ttgatccaac tcattctctt cggttttgcc
     1441 atcaacgcgg acccaaagca cttgccgacg gcggttcttc tcgccgatta cggtcctcac
     1501 ggacgaacac ttctccaggc gatacggaac agcgactact tcgaagtcac ccggcaggtg
     1561 atgagcgagc gagaggcaga ggaggtactc gcgcggggcg acgtgcagtt cgtgatcaac
     1621 attccgtaca acttttcgcg tgacatagtc cgcggtgaga agcctgctat ccttgtcgag
     1681 gccgatgcaa cagacccagc ggcaaccagc aacgccatcg gctctttgcg tgtgttggca
     1741 gcgacggcac tccggcggga tctcaccggg cctctggctt ttctgtccag gaatgatgat
     1801 cccatcgatc tgcgaattca cgcgcgatac aatcccgagg ccatcacgca atacaacatc
     1861 gtgccaggct tgatgggtgt ggtgctgacg atgacgatgg tgatgatcac aggcttggcg
     1921 attacgcgtg agcgcgaacg cggcaccatg gaaaacctgc tctcgatgcc gacgcggccg
     1981 ttcgaggtca tgatcggcaa ggtgcttccc tatattcttg tgggctatgt ccaagtggtg
     2041 ctcattctcc tggcggctca tttgcttttc aaggtaccgg tgtacggcaa cattccgatg
     2101 ctgttcctag ccgcgttgat ctttattacc gccaacctgg cgatgggaat tactttttct
     2161 accatggcac agaaccagtt acaggccgta cagctctcgt tctttttctt cttgccgtca
     2221 ctcctgctct cgggattcat gtttccgttc cgtggtatgc cgcagtgggc acaaacgatc
     2281 ggtgaggtgc tgccgcttac ccactttctt aggatcgtac ggggcatcat gctcaaagga
     2341 aatgggccgg aagaaatcgt gctccagctg tggcagatcg ccctctttgc tgtcgtcgct
     2401 ctggcgatcg gcgtgaaacg gtaccgccag actctagatt agggaattcc tggctccggt
     2461 cacgaaacgg tgcgccatta atatttgagc ttatagaaac cataattcag atggccgaat
     2521 aatgggcggg cttgattctc cgatctatgc tgttgttagc taatggcttt ggcggataaa
     2581 catttaacgg ttgggacgag acctcccggc gctgagtcag tgcgtactga
//
LOCUS       DASTWE010000115         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1521796, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000115 DASTWE010000000
VERSION     DASTWE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521796"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFT30_01280"
     CDS             <1..484
                     /locus_tag="VFT30_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091284.1"
                     /translation="ITYRVEDACTETARQEFDLAVSAWLLVYARNQAELLAMCRGLAS
                     RLKSGGRFVTFTGNPNLYAFTQADYRKYGFSITLTDRAYEGAPIIWTVYLDDSSFQIE
                     NYHLPMQAYETAFIEAGFHDFKVHLPEVSPHPQGVDDGAFWSDYLAQPPAVLIECVKK
                     "
     gene            complement(631..1080)
                     /locus_tag="VFT30_01285"
     CDS             complement(631..1080)
                     /locus_tag="VFT30_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5091285.1"
                     /translation="MALVRWDPFRELEEVSDRLNRMFARPAVSGTNGKETMIVADWTP
                     SVDISETEGEYQIKAEIPDVRKEDVKVTVEDGVLTIQGQRKQEKEEKGTKYHRIERSY
                     GSFARTFSLPDVIETDKVKAEFKDGVLNLYLPKSEKAKPKAIEVKVA"
BASE COUNT          268 a          345 c          309 g          314 t
ORIGIN      
        1 cattacatac cgcgtcgaag acgcttgtac agaaacagct cggcaagagt tcgatctcgc
       61 ggtgtcggct tggctcttgg tctacgcgcg taaccaggca gagctcttgg ccatgtgtcg
      121 tgggctcgcg agccgtttga agtccggggg acgattcgtc accttcacag ggaatcccaa
      181 tctctacgcc ttcacgcaag ccgattaccg gaagtatggt ttcagtatta cgctcaccga
      241 ccgcgcatac gaaggagctc caatcatctg gacggtctat ctcgacgact cctcattcca
      301 gattgagaat taccatttac ccatgcaggc gtatgagacg gcgtttattg aggcaggatt
      361 ccacgatttc aaggttcatt tgccggaggt ctctccacat ccgcagggcg tggatgatgg
      421 ggcattctgg tcggattacc ttgctcagcc tccagcagta ctgatcgaat gtgtgaaaaa
      481 gtaaccgggg ggaacgttgg gctgcccctg ccgctcatcc tagtggaaat cattcgaggc
      541 gattggtgac cgtcgcgtaa ggggaaagat tggtctgaag aaggcccgct gataaggcgg
      601 gccttcttca gaagacctga tttgaccggc ctacgccacc ttcacttcga tggccttcgg
      661 tttcgccttc tccgatttcg gcagatagag attcaatact ccatccttga actcggcttt
      721 caccttgtcc gtttcgatta catcgggcaa ggaaaaggtc cgggcaaagc taccgtaaga
      781 tcgctcaatc cggtgatact tcgttccttt ttcttccttc tcttgcttac gctgaccctg
      841 aatcgtgagc accccatcct cgaccgtgac cttcacatct tcctttctca catcgggaat
      901 ctcggccttg atctgatact ctccttcggt ctcactgata tcaacagatg gcgtccaatc
      961 cgctacaatc atagtttcct tgccgttagt tcccgaaaca gcaggacgcg caaacatccg
     1021 attcaaacga tccgacactt cttccaactc ccgaaacgga tcccatcgta cgagtgccat
     1081 ggtagcctcc ttttagtctt ccctagtgag ctgtcgggtc atcacgctgg cgctgcgccg
     1141 atttatctga aagataaagc gaccgatgga gaagtcaagg gctcggatgg tcttgtaaat
     1201 accgggatta tcgtggtgcg ataggcacgt agcgtc
//
LOCUS       DASTWE010000116         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1069460, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000116 DASTWE010000000
VERSION     DASTWE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069460"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFT30_01290"
     CDS             complement(<1..498)
                     /locus_tag="VFT30_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5091286.1"
                     /translation="MIGDFEALKWGSGTLPRMTMDVLTPKIVDITAEGLSLSGDLTGE
                     ELGLTDTDVSIRGTVAVGLDLRAIERTIYVTGVVEGTAVRQCVRCLKDFDDPLAFSLR
                     VAYEREVKAMNAVAKRDDPRKRKSAAPPEEEGEEQNDDLYYFTGDHLELAPMLREQLI
                     LAAPMH"
     gene            complement(504..752)
                     /gene="rpsR"
                     /locus_tag="VFT30_01295"
     CDS             complement(504..752)
                     /gene="rpsR"
                     /locus_tag="VFT30_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5091287.1"
                     /translation="MDRSENERGGGRLFQRRRPCRFCLEKAPIDFKDAGLLRNFLTER
                     GRIVPRRISGNCMRHQRELTVAVKRARHIAIISFAEER"
     gene            complement(763..1176)
                     /locus_tag="VFT30_01300"
     CDS             complement(763..1176)
                     /locus_tag="VFT30_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5091288.1"
                     /translation="MAGFNKVILMGNLTRNPELRYTPSGTPVASFGLAVSRRFKQGDD
                     LKEEVCFVDIVVFGRQAEHCGQYLSKGNGAIIEGRLQQRRWETEDGQKRSKHEVVAQT
                     VTFMPKRQDAGAGAEPPMHDDPGYDMGEEGLMTGA"
     gene            complement(1163..1510)
                     /gene="rpsF"
                     /locus_tag="VFT30_01305"
     CDS             complement(1163..1510)
                     /gene="rpsF"
                     /locus_tag="VFT30_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5091289.1"
                     /translation="MELYESLFIIRPSVSDEETKALIDKMKNVADKTGAQFIKAENLG
                     KKKLAYEVRRERKGTYAYFYFKAPNNTVGELERAYRLEDNIIKFLTVHHEKPLVERRP
                     VEAAAQESDGGRI"
     gene            1737..2918
                     /locus_tag="VFT30_01310"
     CDS             1737..2918
                     /locus_tag="VFT30_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091290.1"
                     /translation="MDRHIACLQIPAFRIVLARAADSALRNRPVAVALMHTPRAVIRE
                     ISMEAFHEGLQPGMPVDLARLICPGLRMIPPDSSLTQAAHRELQHHISSFAPAWETIR
                     PGSLFLDLTGTTRLFGPPIDTATRISRELTHQQRWPSTIGLAGNKLVSQLAATTLSKP
                     LQVLSIHSGCEQPFLAPLPTMLLPGLHGTQPSRVLQRLEDLHLLTLGAIASVSLAHLQ
                     AAIGPPAGLLRNWALGIDPSPVHPLIAQPMIERSLHLDPDEVDDRLLLGRLYRLSEHI
                     CATLRQRQRMCRHISLTVRHSDHVEQTAHETLPHGTYWESDLQPVLTRLFYRCFSRRV
                     RLTRLMLQVSRLEPPARQLSLFDESESVVLPRSHRLSLALDQIRAKFGDQALSWGRTL
                     R"
BASE COUNT          580 a          925 c          726 g          702 t
ORIGIN      
        1 gtgcatggga gcagcaagaa tcaattgctc ccgcagcatt ggagccagct ccaaatgatc
       61 acccgtaaag tagtagagat cgtcgttctg ctcttcccct tcttcttccg gaggcgctgc
      121 cgacttcctc ttccgcggat cgtctcgctt tgcgacagca ttcatcgctt tgacttcccg
      181 ctcataggcc acacgtaacg aaaacgccaa tggatcgtca aaatctttga gacagcgcac
      241 gcattggcga accgctgtcc cttccacgac tccggtcaca tagatggtac gttcaatcgc
      301 tcgaagatcc agtccaaccg ctacagttcc gcgaatggac acatccgtat ccgtcagtcc
      361 cagctcttct cccgtcaggt ctcccgacaa tgagagacct tctgccgtga tgtccacgat
      421 ttttggtgtc aatacatcca tggtcattct cggcagcgtg ccgctgcccc atttcagtgc
      481 ctcgaaatcg ccgatcacgt tcgctatcgc tcctcggcaa agctgatgat ggcgatatgc
      541 cgagcccgct tgacggccac cgtcagttca cgctgatgac gcatgcaatt cccggaaata
      601 cggcgcggaa cgattcttcc acgctccgtc aaaaaattcc gaagaagccc ggcgtctttg
      661 aagtcgatcg gtgccttttc caagcagaat ctgcagggac gccttcgctg gaataaccgt
      721 cccccgccac gttcgttttc gcttcggtcc attactgcct cctcatgctc ctgtcattaa
      781 cccttcttca cccatatcat agccggggtc gtcgtgcatc ggaggttccg cacccgcccc
      841 cgcgtcttgg cgctttggca tgaacgtcac ggtctgggcg actacttcat gtttgctgcg
      901 cttttggcca tcctcggtct cccatcggcg ctgctgcaac cggccttcga tgatcgcccc
      961 attgccttta ctgagatact gtccgcaatg ttccgcctgt ctgccgaata ccacgatatc
     1021 gacgaagcag acttcttcct tcagatcgtc accctgttta aaccgacggc tcaccgccaa
     1081 gccaaaactc gccaccggtg tcccgctcgg cgtataccgg agctcagggt tacgggtgag
     1141 gttccccatc agtatgactt tgttaaatcc ggccaccgtc tgactcctgt gccgcagctt
     1201 ccaccggacg ccgctccacc aagggttttt cgtgatgaac cgtcagaaac ttgatgatgt
     1261 tgtcttccaa tcgatacgcc cgctcaagct cgccgactgt attgttcggc gccttaaaat
     1321 agaaataggc gtaggtaccc ttccgctcgc gacgtacttc gtaggcgagc tttttcttcc
     1381 ctaaattctc ggctttgatg aattgggcgc cggtcttatc ggcgacgttt ttcatcttgt
     1441 cgatgagcgc cttggtctcc tcatcggaga cggacggacg aataataaac agagactcgt
     1501 agagctccat gcagcgatcc tcctggacaa aggcccccga cccagtcggg agcgaggtgt
     1561 agggtgccta tttggcgtag agaacggtgt ggacggtagc acagggtgga aatggctgtc
     1621 aacaaaagcg ggggctgact tcgcccactc acccgctttc actcgcctgc tcggcttttc
     1681 tacgtacttg actggggtta tttttattgt aaccattggc tactaacttg accgccatgg
     1741 atcgccatat tgcctgtcta cagattcctg cttttcgaat cgtattggcc cgtgccgctg
     1801 atagcgcgct gcggaacaga ccggtagccg tagcactgat gcatacgccg cgtgcagtga
     1861 tccgagagat ttctatggaa gcatttcacg agggtctaca accggggatg cccgtggatc
     1921 tcgcaaggct gatctgccct ggtctgcgga tgatccctcc ggattcatca ctcacgcaag
     1981 ccgctcatcg cgagctgcaa caccatatct cgtcctttgc accggcttgg gaaacgatca
     2041 ggcccggatc gctctttctg gacctcaccg gcacaactcg actcttcggc cctcccatcg
     2101 atacagcgac ccgcatcagt cgagagttga cccatcagca gagatggccg agtacgatcg
     2161 gattggcggg aaacaagctg gtctcgcaac tggccgcgac cacattatcg aaaccactcc
     2221 aagtgttatc gatccactcc ggctgtgagc aaccgttcct cgctccgttg ccgaccatgt
     2281 tgcttcccgg ccttcacggt acccaaccct cacgggtctt acaacggcta gaagatctac
     2341 acctccttac acttggcgca atcgcctctg tgtcgttggc acatctgcaa gccgccatcg
     2401 gccctccggc cggtttgctg cgcaactggg ccttggggat cgatccttcc ccggtacatc
     2461 cactaatcgc acagccgatg atcgaacgct cccttcatct cgatccggat gaagtggatg
     2521 atcggctctt gcttggtcgg ctataccgat tatcggaaca tatttgtgca acacttcggc
     2581 aacgacaacg gatgtgccgg cacatctcgc tgactgttcg tcatagcgac catgtcgagc
     2641 agaccgctca tgagacgttg cctcacggca cctactggga gagcgatctc cagcctgtat
     2701 tgacacgcct cttctaccga tgtttcagcc gacgggtgcg tctcacacgg ctgatgcttc
     2761 aggtcagccg gctggaacca ccagcccggc aactttcgct cttcgacgag tcggaatctg
     2821 tcgtgcttcc ccgctctcac cgtctctcgc tggctctgga tcagattcgc gcaaaattcg
     2881 gcgaccaagc cctgtcctgg ggaaggacgt tacgatgacg acggctcagt tcg
//
LOCUS       DASTWE010000117         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_164554, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000117 DASTWE010000000
VERSION     DASTWE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164554"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..893
                     /locus_tag="VFT30_01315"
     CDS             120..893
                     /locus_tag="VFT30_01315"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU5091291.1"
                     /translation="MTDDRLKIAGREFKSRLWVGTGKYKDFVETQKAIEASGADVVTV
                     AVRRVNITDRSKDNLLDYIDPKKYTILPNTAGCYTVEDAVRYSRLARAAGVSDLVKLE
                     VLGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAAPI
                     GSGLGIRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGAQDP
                     LAMAEAMKYAVHAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            1082..1708
                     /gene="thiE"
                     /locus_tag="VFT30_01320"
     CDS             1082..1708
                     /gene="thiE"
                     /locus_tag="VFT30_01320"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5091292.1"
                     /translation="MPSVNFRLLLVTDRTQAGGRSLPSLIRQAVSAGLSAVQLRERDL
                     STREFLPLAQEIQSITASHAVSLIINDRVDLMLALNLDGVHLRSDSLLPFPVRRLIGP
                     RRLIGVSTHSVDDVRYANQGGADYVVFGPIFETPSKRSFGAPLGLDLLADACRQSSIP
                     VLAIGGITCERVRDVRRAGAHGVAVIGALLTRDDIGEAVREFNHALET"
     gene            1940..>2738
                     /locus_tag="VFT30_01325"
     CDS             1940..>2738
                     /locus_tag="VFT30_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5091293.1"
                     /translation="MIHKNDEHAREIEKLRVQIQSMEEEIRRLYQSRHQLDQANKQNE
                     KLVATLQEAKAQIEALRTEVEKLTAPPSAYAIFSSMNADGTGNVYVSGRKMKVNLHPS
                     IKRNELRKGQEVVLNEALNVIEVKGFDSQGEVVRLKDVLEGGRALVTLHFDEEKVAEL
                     GDPLLAERLSVGDHLLYDPRSGYVIEKLPKSEAEELVLEEVPDVDYEHIGGLQKELEQ
                     VRDAVELPFLHPHVFSEYKLSAPKGVLLYGPPGCGKTLIAKAVANSIA"
BASE COUNT          604 a          704 c          804 g          626 t
ORIGIN      
        1 gtcgagattc ttggctttat cggcggcggc gccacggcaa agaattgagc cggttctcgc
       61 attttcttta aggagcctat ggttagtggc ttcgtgacta tgttctggag aggataacga
      121 tgacggatga tcgactaaaa attgcggggc gtgaattcaa gtcccggcta tgggtcggga
      181 cgggaaaata taaagacttt gtcgagacgc aaaaggccat tgaagcgtcc ggtgccgatg
      241 tcgtgacggt cgcggttcgt cgagtaaata tcaccgatcg atcgaaagac aatctgctcg
      301 attacatcga cccgaagaaa tacacgattc ttcccaacac ggctggatgc tataccgtgg
      361 aggatgcggt acgatactca cgcttggctc gcgccgccgg tgtttccgat ttggtgaagt
      421 tggaagtgct cggtgacgag aagacgttgt ttcccgatac ggccggtttg atcgaagccg
      481 ccaagattct cattaaggaa ggatttatcg tccttccgta cacgaatgac gacccgattg
      541 tcgcgaagaa actcgtggat atcggatgtc cggcggtcat gcctttagct gcccccatag
      601 gatccgggct cgggattcgt aatccgtaca acctgaaaat catcatggaa accgtcaagg
      661 tgccgattat cgtcgatgcc ggtgtcggaa ccgcgtcgga cgccgctttg gcgatggaat
      721 acggcgccga cgcggtgctc atgaatacgg ccattgccgg tgctcaggat ccgcttgcca
      781 tggcggaagc gatgaagtac gcggttcatg ccggccgtct ggcttacaag gccggtcgca
      841 ttcccaggaa actctatgcg acggccagta gcccgatcga agggatgctc tagcaggatg
      901 ctgaaaaagt ctgccagcgg cgttctcgca tcgctcagag gctcaacgta ccgaagcgta
      961 cgcctcgcca cttcgcttgc tgtggtcttg ctggacagcc tttttgagca tcctgaaggc
     1021 attctggcgt tggtagtgag ttctcgcgtt cagcatcgat cgggacatac attcttcgca
     1081 catgccctcc gttaacttcc gtctgctgct cgttactgac agaactcaag caggcgggcg
     1141 gtcccttcca tcacttatcc gacaggcagt cagcgccggg ttgtccgctg ttcaactgcg
     1201 ggaacgagat ttatcgacgc gcgaatttct tccgctcgcg caggagattc agtcgattac
     1261 ggcgtctcat gccgtttctt tgatcatcaa tgatcgcgtc gatctgatgc tggcgctgaa
     1321 tttggacggc gtgcatcttc gatccgacag tctgcttccc ttccctgtgc gtcgcctgat
     1381 cggaccacgt cgattgatcg gagtttccac acattcggtc gatgatgtgc gatatgcgaa
     1441 tcaggggggt gccgattatg tcgtctttgg tcccattttt gagactcctt cgaaacgttc
     1501 attcggggcc ccacttgggc tcgatctgct ggccgatgcc tgccgccaat catccatccc
     1561 cgttcttgcc attggaggaa tcacatgcga acgagtgcgt gacgttcgtc gagccggtgc
     1621 tcacggcgtt gccgtgatcg gagcgctgtt gactcgtgat gacatcggcg aagctgtccg
     1681 agagttcaac catgcgttgg agacctgata cggcggtgca tggcagggag gagtcttgca
     1741 cgagatcgtc cgttggagtt gaccacccct aggtcgggtg ttagaatccg gtcaagattt
     1801 attcgcactg acgctcgatg ccgcgagctc ttcgtgtcgt ctcgatatgg cggaaaacaa
     1861 gggtcagcca aatcgactcc ggtccctccg agattctgtc aagaagatta cgaagcggct
     1921 gtccgagggg aatggggaga tgattcacaa gaacgacgaa cacgcgcgcg agatcgaaaa
     1981 gcttcgcgtt cagattcagt cgatggagga agaaatccgt cggctctacc aatcacgaca
     2041 ccaactcgat caagccaata aacagaacga aaaactcgtg gccacgctgc aggaagcgaa
     2101 ggcgcagatc gaagcgctgc gtaccgaggt ggaaaaactg acggcgccac cgtcggcgta
     2161 tgctattttt tccagtatga atgcggacgg taccggaaat gtctatgtgt cgggacggaa
     2221 aatgaaggtc aaccttcatc cctcgatcaa gcgcaatgag ttgcggaagg gacaagaagt
     2281 cgttctgaat gaggcgctca acgtgatcga ggtcaaaggg ttcgacagtc agggagaggt
     2341 cgttcgactc aaagacgtgt tggaaggggg acgggcattg gtcacactcc atttcgatga
     2401 ggagaaggtg gccgagctcg gtgacccact gcttgctgaa cggctgagtg taggggacca
     2461 tctgttgtat gatcctcgct ccgggtatgt cattgagaag ttaccgaagt ccgaagccga
     2521 agaactcgtg ttggaagagg tcccggatgt cgactatgag cacatcggag gcctccagaa
     2581 agaattggaa caggtacgcg atgccgtcga acttccgttc ctgcatccgc acgtgttctc
     2641 cgagtataag ctcagcgcac cgaagggggt tctgctttat ggcccacccg gttgtgggaa
     2701 gacgctgatc gccaaagccg tcgctaattc gatcgcca
//
LOCUS       DASTWE010000118         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_875487, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000118 DASTWE010000000
VERSION     DASTWE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875487"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..>1724)
                     /locus_tag="VFT30_01330"
     CDS             complement(278..>1724)
                     /locus_tag="VFT30_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091294.1"
                     /translation="LSPEAARDRCSAIRTRLGHANVFGSPSAVAFQQPSSGFDAANSW
                     RVSPCPFYLSPDQLQFFIDLGPQLLSFYRGLNRLYNESVKGIQPAWVAGYLDQGKPEA
                     LMQYSRMKRFRDALPAIIRPDIIPTQDGMVITELDSVPGGIGLTACLSYIYHDLNSGD
                     SRLIGGRDGMVHGFARMLRSLRGEREGCIAILVSEESKDYRPEMSWLAAQLREEKVPA
                     WCVEPHEIRFTEEGLRLHADGNEQPIGIVYRFYELFDLLNIPKAELVQYAVKKGRVSV
                     TPPYKPALEEKSAFALLHHPVLRPFWEKELGADCMLHLSAIMPKTWLLDPAPLPAIAT
                     IPGLRIGGRAVANWSDLETATQKERHFVIKPSGFSALAWGSRGVSIGHDMPQNAWAEA
                     LRNALASFPTTPYILQEFHKGRLFEMDYLDEGSQAIVRMSGRARLSPYYFVSGDKVEL
                     TGILATICPADKKVLHGMKDAIMAPCALRSN"
BASE COUNT          386 a          477 c          495 g          366 t
ORIGIN      
        1 ggacaccgcc cgctcacgaa gacaccgccg cctcaccgac tcggcggcgt ccacaaacgt
       61 gacgctcatt attcttcgcg tcgtggacct cgcgggcgtt cacaaacgtg ccgtgcttta
      121 ttcagcgcgg cgtgaacctc agaagctcaa cataccgaac agcatgcacc tagcctgagg
      181 ccttgcttgt ggagagggca tctcggcacc ggggtcgggc gggtgagagc ggcggcgttt
      241 ttgaacatcc tgctgaccat tcattgtttt tgaggagcta gttggatcgt agagcgcaag
      301 gagccataat cgcgtctttc atgccgtgaa ggactttttt gtcggctggg cagatcgtcg
      361 ccagaatccc ggtgagttcc actttatcgc ccgagacaaa atagtaggga gacaaccgtg
      421 cccgtccgga catgcgtacg attgcctgac tgccttcatc cagatagtcc atttcgaaca
      481 gccgcccctt gtggaactct tgcaagatat agggtgtggt cgggaaggat gcaagcgcgt
      541 tccggagcgc ctcggcccat gcgttctgag gcatgtcatg cccgatcgac acgccgcgac
      601 ttccccatgc gagtgcggaa aatccggaag gtttgatgac gaaatggcgc tccttctgcg
      661 tggccgtctc gagatcggac caatttgcaa ccgcacgtcc accgatccgt aggccgggga
      721 tcgtcgcaat cgcaggaagc ggtgccggat cgagcagcca ggttttcggc atgattgcgc
      781 tcaaatgaag catgcagtcc gctcccaatt ccttttccca gaaggggcga agtacgggat
      841 ggtggagcag cgcgaaagcg gatttttctt ccagcgccgg tttgtagggc ggcgtaacgg
      901 acaccctgcc tttcttcacg gcatattgaa cgagctcggc tttgggaatg ttcaacaggt
      961 cgaacagttc atagaaacgg tagacgatac caatcggttg ttcgttgccg tcggcgtgca
     1021 atcgaagccc ttcttctgta aaacgaatct catgaggctc gacacaccaa gcgggcactt
     1081 tctcttcgcg gagttgagcg gccaaccagg acatttccgg ccgatagtcc ttagattctt
     1141 ccgagaccag gatcgcaata catccctcac gctcgcctcg caaagatcgc agcatgcgag
     1201 caaatccatg taccatccca tctcgtcctc cgatgagtcg tgaatcaccg ctattaaggt
     1261 cgtgatagat gtacgatagg caggcagtaa gcccgatacc acctggaacc gaatccagct
     1321 cggtaatgac catgccgtcc tgtgtgggga taatatcggg cctgatgatg gcgggcaggg
     1381 catcgcgaaa ccgtttcatc cggctgtact gcatcaaggc ttccggcttg ccttgatcga
     1441 gatatccggc aacccaagct ggttgaattc ccttgacgct ctcattgtag agccgattca
     1501 gcccgcgata gaacgagagc agttgtggcc ccaaatcgat gaagaattgc agttgatcgg
     1561 gagacaggta aaacggacag gggctgactc gccaagagtt tgccgcatca aagccggaag
     1621 acggctgctg aaaagctacg gcggatggag atccaaacac attggcatgg cctaatcgtg
     1681 tacggatggc ggagcagcga tcacgtgctg cctcgggaga gagc
//
LOCUS       DASTWE010000119         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_907919, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000119 DASTWE010000000
VERSION     DASTWE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907919"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1085
                     /locus_tag="VFT30_01335"
     CDS             102..>1085
                     /locus_tag="VFT30_01335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5091295.1"
                     /translation="MKFMYHLVPTIPATPQERERLAPIAHRTDKTQEMLEEMIDLAQL
                     AEAVNFDAVTYSEHHFYTEGLEAGATPIPHLLNLLFHTKRIKIGPVGFVLPTWDPIRL
                     AEDVAWADHMSKGRVLVGLARGFFPRWVNVLGQKYGVQPGTMGPEAELRNREVFQELF
                     EVLKLSWKDEPFSFKGKYYQVPFPAEGHDWAAREATRRYGAPGEVDDTGWLRKISPVP
                     KTYQKPHPPLFQALTTNEETIRWAAREGIVPMMFLPFPDLCIKGASVYTEEANKAGHA
                     LRLGQNIGLARLVYIGKTRAEAMQRARDGSIFLFSRFHAKFDKNIPTSIEPL"
BASE COUNT          262 a          276 c          308 g          239 t
ORIGIN      
        1 ttaaggcgac gaggaacaag agacagcgag aactaaaaag aaggagcctt ttctgttccc
       61 ttccacctat aacttgtaac ctaaggtaaa ggaggacctt catgaaattc atgtatcatc
      121 ttgttccaac tataccggca acgccgcaag agcgagaacg cctcgcaccg atcgctcatc
      181 gcacggataa gacgcaagag atgctcgaag agatgattga ccttgctcag cttgccgagg
      241 cggtgaattt cgatgcggtg acgtattccg agcatcattt ctacaccgag gggttggaag
      301 cgggagcgac gccgattccc catctgctca atttgttatt tcacaccaaa cgcatcaaaa
      361 ttgggccggt tgggtttgtc ttaccgacgt gggacccgat ccgccttgcc gaagatgttg
      421 cctgggcaga ccatatgtcc aaaggtcgag tcctggtcgg tttggcacgt ggtttctttc
      481 cgcgatgggt gaacgtgctc ggacaaaagt acggcgttca accgggcaca atgggaccgg
      541 aagcggagtt gcgtaatcgc gaagtctttc aagagctctt tgaggtgctg aaactgtcgt
      601 ggaaagacga gccgttctcg ttcaaaggaa aatattacca agttccgttt cccgccgagg
      661 ggcatgactg ggcggcgcgc gaagcaacgc gacgttacgg tgctcctgga gaggtggacg
      721 acaccggctg gctccgcaag atctcgccag tgccgaagac ttaccagaaa ccgcacccgc
      781 ccttatttca ggcgctgacg acaaatgaag agaccattcg ctgggcagcg cgcgagggta
      841 ttgtgccgat gatgttcctg ccgttccctg atctgtgcat caaaggggcg tcggtgtata
      901 cggaagaagc caacaaggct ggacatgcgt tacgactcgg gcagaacatc ggcttggcgc
      961 ggctggtgta cattggcaag acccgcgctg aagcgatgca acgggcacgt gacggatcga
     1021 tcttcttgtt tagtcgattc catgccaagt tcgacaaaaa tattcctact tctattgagc
     1081 cgtta
//
LOCUS       DASTWE010000120         1420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_907956, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000120 DASTWE010000000
VERSION     DASTWE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907956"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..484
                     /gene="panD"
                     /locus_tag="VFT30_01340"
     CDS             128..484
                     /gene="panD"
                     /locus_tag="VFT30_01340"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEU5091296.1"
                     /translation="MFRQMLRSKIHRATVTGAHLEYEGSLTIDQDLMEAAGILPYEAI
                     ICSNLNNGERFMTYAINGTRGKGEIILNGPTARKAAVGDQIIIFCYEYYGEEEIKKHA
                     PKIVRVNDKNRIVMVS"
     gene            556..>1420
                     /locus_tag="VFT30_01345"
     CDS             556..>1420
                     /locus_tag="VFT30_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU5091297.1"
                     /translation="MSLHKLTLVELQKQFKAGEVTATEIVRAYFLRISQVEPKMKAFV
                     TQTKESAFLQAEALDRKLKEWRKTKPLTGMPLAIKDNICTDGVHTTCSSRMLQNFIPP
                     YDATTVAKLRAQEYILLGKTNLDEFAMGSSTENSAFGPSRNPWNLHCVPGGSSGGSAA
                     AVAAEECVAALGSDTGGSIRQPAAFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITR
                     DVSDAAFLLQAIAGHDPMDSTSVDRSVPDYMKALQKRDLKSLKIGVPVEFFTEGLDPD
                     VEQAVRAAIGEL"
BASE COUNT          351 a          343 c          425 g          301 t
ORIGIN      
        1 gcgcctgcgc gtgaagctga cgggtttgtt gtacccaaaa ttctagagga gcgttaatcg
       61 tcagacagcc gactcatacg tggacgacaa cgaatgatga cagcgattga cgaataaggg
      121 gagatagatg tttcgacaaa tgctacgttc gaaaattcac cgtgcgacgg tgacgggcgc
      181 ccatctcgag tatgaaggca gtctgacgat cgatcaagac ttgatggaag cggccgggat
      241 cctgccgtat gaggcgatta tctgctcgaa cttgaataac ggcgagcgat tcatgaccta
      301 cgcgatcaat ggtacgcgag ggaaagggga gatcattttg aatgggccca ccgcgcgaaa
      361 agcggcggtc ggagatcaga tcatcatctt ttgttatgag tattacggcg aagaagagat
      421 caagaagcac gcacccaaga tcgttcgggt gaatgataaa aatcgaattg tgatggtgag
      481 ctgatctact aggacagacc aatcgatgat gtctgttggt ccgtgttcat ctcacccgtc
      541 ggtagtggga gattcatgtc ccttcataaa ctgaccctcg tcgagcttca gaagcaattc
      601 aaggcagggg aagtgacggc gacggagatc gtgcgcgcct actttctgcg catcagccaa
      661 gtagagccca agatgaaggc cttcgtgacc caaacgaaag agtcggcatt cctgcaggct
      721 gaggccttgg accggaagct caaggaatgg aggaagacca aacctcttac cggaatgcct
      781 cttgccatca aggacaatat ctgtacggac ggcgttcaca cgacctgcag ctctcgaatg
      841 ttgcagaact tcataccgcc ttacgatgcg acgaccgtcg ccaagttgcg tgcgcaagag
      901 tacattttgt taggcaagac gaatttggat gaattcgcga tggggtcgtc aacggaaaac
      961 tccgcgttcg gccccagccg aaatccctgg aacctacact gtgtgccggg aggatcaagc
     1021 ggagggtctg cggcggcggt ggcggcggag gaatgtgtgg cggcacttgg ctcagacaca
     1081 ggtgggtcca tcaggcagcc ggcagccttc tgtggcgtgg tgggactgaa gcctacctat
     1141 ggacgagttt cgagatatgg cttggtcgca ttcgcttcct cgctggatca aatcgggccg
     1201 attacgagag atgtgtctga cgcggcattt cttctgcagg ccattgcagg tcatgatccg
     1261 atggactcca cgtcggtcga ccgctccgtg ccggattata tgaaagcgct gcaaaaacgg
     1321 gatctcaaat ctctaaagat cggcgtaccg gtcgaatttt ttaccgaagg actcgacccg
     1381 gacgttgagc aggcagtcag agcggccatc ggggagttga
//
LOCUS       DASTWE010000121         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_552529, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000121 DASTWE010000000
VERSION     DASTWE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552529"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFT30_01350"
     CDS             complement(<1..353)
                     /locus_tag="VFT30_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5091298.1"
                     /translation="MKPVHSDAFIAGASAPAASPSNADVFPASFAQQRLWFLSQLEPD
                     SASYNTALAVRLRGPLNREATAQSLNEIVRRHEVLRTTFDEIDGELVQVIAEDLPMDL
                     PVVDFSLEPPSERQTS"
     gene            complement(350..1381)
                     /locus_tag="VFT30_01355"
     CDS             complement(350..1381)
                     /locus_tag="VFT30_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU5091299.1"
                     /translation="MQANHNNSAPMSVEETCREVTGLPSCRVSYLSSNTPLPVVVNPM
                     EKRSLAEYRDVIEQMVNTRLSTVGGLLFRGFPIESVADFEAFVKMISPDLASYEFGST
                     PRSQVQNQIYTSTEYPPHQHIPLHNEQAYTTEWPMKIWFYCAQASPEGGYTPIADSRE
                     VYRHIPIRIRERFIEKGVMYVRNYGNGLDVPWNKVFNTMDRRTVEQYCRSAGIECEWK
                     ADGELRTRQVCQAVAVHPLSGETVWFNQAHLFHVSNLEPAVREALLSFVDEEDLPRQA
                     LYGDGTPIETAVLDEIRGVYQCHAVQFPWQKGDVLMLDNMLAAHGRTPFKGPRKILVA
                     MSGSNKGSQ"
     gene            complement(1439..>2117)
                     /locus_tag="VFT30_01360"
     CDS             complement(1439..>2117)
                     /locus_tag="VFT30_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5091300.1"
                     /translation="GGASVFRSWADNEFLADVEVCAVQLPGRETRITESPVGDLRRLV
                     HMLCEALEPYLDRPFVFFGHSIGALVSFELARELRRMRGIEPSYLFVSGCPAPHLPDS
                     ERICDLPENEFLERVGRFNGTPPEVLNHPELMQLVLPALRADFSLRDRYVYRDEPPLN
                     CPIIAFGGMSDQHVNGVLLGSWRQHTRERFQLWLVQGDHFFVRSAQGQLMEILSSLLS
                     QYRGIVR"
BASE COUNT          485 a          646 c          548 g          438 t
ORIGIN      
        1 gaagtctggc gctcggatgg tggctcaagg ctgaaatcca ccaccggcaa gtccatcgga
       61 aggtcttcgg cgatgacttg aaccaactcg ccgtcgatct catcaaaagt cgtgcggagg
      121 acttcatgcc gccggacgat ctcgttgaga ctctgcgccg ttgcttctcg gttgagcgga
      181 cctcgcaacc gtaccgccag cgccgtgttg tacgaggcgc tgtccggttc cagctgggag
      241 agaaaccaca accgctgctg cgcaaaactc gccggaaaga cgtccgcatt cgatggagaa
      301 gcggccggcg ccgacgcacc ggctatgaat gcatctgaat gtacaggttt cactgtgaac
      361 ccttgttcga tccggacatc gccaccagaa tctttctcgg tcccttgaac ggcgtgcgcc
      421 catgcgccgc cagcatattg tccaacatca acacgtcgcc tttttgccaa gggaactgaa
      481 cggcgtggca ttgatagacg ccccggattt cgtcaagcac cgccgtttcg atcggcgtcc
      541 catctccgta gagggcttgc cgaggcagat cttcctcgtc gacaaaggac agcagcgcct
      601 ctctcactgc cggctccaga ttcgacacat ggaatagatg ggcctgattg aaccagactg
      661 tttctccgct cagtggatgg actgcgaccg cttgacagac ttggcgagtg cgcaattctc
      721 cgtccgcctt ccattcgcac tcgatgcccg ccgaccggca atattgttca acggtcctgc
      781 gatccatcgt gttgaacacc ttgttccaag gcacgtccag tccattcccg tagttccgca
      841 catacatcac gcccttctcg atgaagcgct ctcggatgcg gattggaatg tgccgataca
      901 cctcgcggct gtccgcgatc ggcgtatagc ctccttccgg cgacgcttgc gcgcaataaa
      961 accagatctt catcggccat tcggtggtat aggcctgttc gttatgcaga gggatgtgct
     1021 gatgcggagg atattcggtc gacgtataaa tctgattctg gacctgcgac cgtggcgtcg
     1081 atccgaactc gtaggacgcc agatccggcg aaatcatctt tacgaaagct tcaaagtcag
     1141 ctaccgattc aataggaaac cctctgaaca gcagtcctcc gacggtcgaa agacgcgtat
     1201 tcaccatctg ctcaatgacg tctcggtatt cagccaacga ccgcttctcc atgggattga
     1261 cgacaacagg aagcggggtg ttagaggaca agtagctcac acggcatgac ggcagaccgg
     1321 ttacctcccg acatgtttct tcgacagaca tcggtgcaga attattatga ttggcctgca
     1381 tgaagctcct ccacatgcgt cgacgcaacg aatctttccg tgactgcggc cgccaccgtc
     1441 atcgcacaat ccctcgatat tgcgataaca atgaggagag aatctccatc agctgtccct
     1501 gcgcgctccg cacaaagaaa tgatctcctt gaactagcca cagttgaaat cgctcgcgag
     1561 tatgctgccg ccagcttccc aataggaccc cgtttacatg ctgatcgctc atccctccga
     1621 atgcgatgat gggacagttc aatggcggct catcacgata aacgtatcga tcgcgaagcg
     1681 aaaaatcggc gcgaagcgcg ggaagtacca attgcatcag ttccggatga ttgaggactt
     1741 ccggcggagt gccgttgaac cgacccaccc gttcaagaaa ttcgttttca ggcagatcac
     1801 aaatccgctc cgaatccggg agatgtggag ccggacaacc ggacacgaac agatagcttg
     1861 gctcgattcc acgcatccgg cgcaactccc gggccaattc gaaactcacc agtgccccaa
     1921 tgctgtgacc gaaaaagacg aacggccgat ccagataagg ttccagcgcc tcgcacaaca
     1981 tgtgcaccag ccggcgcaaa tcaccgacag gcgactcggt gatcctggtc tcccgccctg
     2041 gaagctggac ggcgcaaact tcgacatccg ccaagaactc attgtctgcc cagcttcgaa
     2101 acacagaggc tcccccg
//
LOCUS       DASTWE010000122         1239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1392770, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000122 DASTWE010000000
VERSION     DASTWE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392770"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VFT30_01365"
     CDS             complement(<1..848)
                     /locus_tag="VFT30_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5091301.1"
                     /translation="MPQPEAATLFIAASEHDSNLYYATRFMAPDPFIYLEIKGERLMV
                     MSDLEMDRARTQASVDRVLSYSEIEQKAKKQGIKSPTTVDIVHLVLKESKVRRLLVPA
                     NFPFIYATRLQELGYSLKPKRDPFYEQRVVKTAEEVRSIELSQRATEDAVTSAHDLLR
                     RATIRDGELWFDGAVLTSERVKQLINVKLMERNCVAQHTIVAGGEQACDPHNEGSGPL
                     PAHRSIIFDVFPRSATSRYFADMSRTVVLGTASPELKRLYGIVKDAQEDAIGKIKDGA
                     DGMKIH"
     gene            1023..>1239
                     /locus_tag="VFT30_01370"
     CDS             1023..>1239
                     /locus_tag="VFT30_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091302.1"
                     /translation="MGELFQHPVEDANMGAGVGQEWEAVKKWWSSRIKAGHGIIGAGE
                     RVISFGRTSDSSSGVTKSIGNVRVFVKT"
BASE COUNT          283 a          328 c          331 g          297 t
ORIGIN      
        1 tgatgaatct tcataccgtc tgcgccgtcc ttaattttgc cgatggcatc ttcctgggca
       61 tccttcacga tgccatacaa ccgttttaat tccgggctcg ccgtcccgag gactaccgtg
      121 cgcgacatgt cggcaaaata gcggctcgtc gctgagcgag ggaatacatc gaagataata
      181 ctgcgatgag ccggcaacgg tccgctccct tcattgtggg gatcacaggc ctgctcccca
      241 cccgccacga tcgtatgctg ggccacgcag ttccgctcca tgagtttgac attgatcaac
      301 tgcttgaccc gctcagatgt caacaccgct ccgtcaaacc aaagctcccc gtccctgatg
      361 gtcgcccgtc gcagcagatc atgcgcagag gtcaccgcat cttcagtggc tcgctgagag
      421 agttcgatag atcgaacctc ctccgcggtc ttcacgacac gttgttcgta gaacggatcg
      481 cgttttggtt tgagactgta tcccagctcc tgtaaccgcg tggcgtagat gaaggggaaa
      541 ttagccggga ccagcagacg tctgactttg gactctttca aaacgaggtg cacgatatcg
      601 accgtcgtcg gacttttgat cccctgtttc ttcgccttct gttcgatttc agagtaggac
      661 agcactcgat ccactgaggc ttgggtcctg gcacgatcca tctccagatc gctcataacc
      721 atcaaccgct caccctttat ttcgagataa atgaacgggt ctggcgccat gaagcgtgtg
      781 gcatagtaca gattcgagtc atgctcactg gcggcgatga ataatgtggc cgcctctggt
      841 tgtggcatcg cgctgcgatt catggataat aactagtgag agtatggagc gggatggaat
      901 ctcaccaaaa ataacaacca gacttcaggg tcccaaaaat tcgcccggcg ctgtctcagg
      961 aaacttgaac gtcgaggcgt acgcaatttg tgcgttgagg cattcaaacg actgagaacg
     1021 atatgggcga gctttttcaa catcccgttg aagatgctaa catgggggca ggggtcggtc
     1081 aagaatggga ggcggtcaag aaatggtggt cgtcaagaat caaggctggt cacggcataa
     1141 taggggccgg cgagagagtc atatcttttg gtcgcacttc ggattcttca tctggtgtca
     1201 ccaaatcaat cggcaatgtc agggtgtttg ttaagacat
//
LOCUS       DASTWE010000123         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_35464, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000123 DASTWE010000000
VERSION     DASTWE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35464"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFT30_01375"
     CDS             complement(<1..592)
                     /locus_tag="VFT30_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091303.1"
                     /translation="MTRSRLSAHTQSRTWITVAKTLGRSQDHCVHHRATTSVTLIVLF
                     LMLVPSLAVAEGKVSPILLADVVSQQAGQLAPIDSDEGALTLFTTTIGPVLGLKDAAG
                     ALGTKKLPSKMVKELGLAELSKSVHELMAALATWQLADSIRHDAEPSPVVLSLSSARQ
                     DWLRGHSRSASLSSLLRLMQEDQTLLTSQTPANPQNT"
     gene            complement(589..1389)
                     /locus_tag="VFT30_01380"
     CDS             complement(589..1389)
                     /locus_tag="VFT30_01380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5091304.1"
                     /translation="MPTTQTAPFTLAQIGTGTARFPSGVPIPTHTDQMVDPYFKSKVP
                     KEHQVDCLLFWPQQAGTYPGVVLLHDRWGLTGQMKDLGAQLACEGYMVIIPNLYGRLG
                     GMVTANDDVAAALLERQNDAHIITDINSCCEYLNTRHFAKRNIHGVVGYGMGGSYALR
                     FACHRKRLRAAVSYYGKAVTPKELMKDLFSPLLYHQAGKDTWATQDDVEQLRSASAEY
                     AKRVEIHFYPEASHAFCNDMKPHAYDPDSSALAWERTASFLKSCFQGT"
     gene            1617..>2196
                     /locus_tag="VFT30_01385"
     CDS             1617..>2196
                     /locus_tag="VFT30_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5091305.1"
                     /translation="MNKDCIGVGLIGVGRHGMRYAQHLIQDLPTASLRAVCRRHPEQV
                     FHVPGAESVKVYGDAPSLIADPTVDVVVVVTPPIYSSEICRLAAQARKPLLIEKPLAT
                     TATDARMMVAQACEAGVPLMTAQTLRFDHTIQHMKTLQPYIGRSQQLDLVFNIEIRKT
                     APDHADGYGKRGALLEIGVHMLDLVRFLTGEEV"
BASE COUNT          463 a          572 c          643 g          518 t
ORIGIN      
        1 ctgtgttttg cggattggcg ggtgtttgag aggtcaaaag cgtctggtcc tcttgcatga
       61 gacgaagcag gctggagagt gaagccgacc ggctgtggcc tcgcaaccaa tcttgtcgtg
      121 cggaagaaag tgagagcacg acaggggatg gttctgcatc atgacggatg gaatcggcaa
      181 gctgccatgt agccagcgct gccatcagct catggacgga tttggataat tccgccaacc
      241 ccagttcttt gaccatttta ctcgggagtt tcttggttcc caatgctccc gctgcatctt
      301 tgagcccgag gacagggcca attgtcgttg taaacagtgt cagggctccc tcgtctgaat
      361 cgatcggagc cagctggccg gcctgttgac tcaccacgtc ggccaacagg atcggagaca
      421 cttttccctc cgccacggca agagacggga cgagcatcaa gaacagcacg atcagggtga
      481 cactggtcgt ggcacggtga tgtacgcagt gatcttggct ccggccgaga gtttttgcga
      541 ccgtaatcca tgtgcgagac tgagtgtgcg ctgagagacg acttcgggtc atgttccttg
      601 gaagcaagac ttcaagaagc ttgcggttcg ctcccaggcg agggccgaac tgtcgggatc
      661 gtaggcatgc ggtttcatat cattacagaa agcgtgggag gcttcggggt agaagtgaat
      721 ttcgactcgc ttagcatatt cggcagaggc gctgcgcaat tgctcgacgt cgtcttgagt
      781 ggcccaggta tcctttcccg cttggtgata cagcagcggt gaaaagaggt ctttcatcaa
      841 ctctttggga gtgaccgcct tgccgtagta tgagacggcc gcgcgcagcc gtttccgatg
      901 gcaggcgaaa cggagcgcat aggaacctcc cattccatag cccacgactc catgaatgtt
      961 cctcttcgcg aagtggcgtg tattgagata ttcacaacag gaattgatat ccgtgatgat
     1021 atgcgcgtcg ttctgacgct ccagcagagc agccgccacg tcgtcgttgg cggtcaccat
     1081 tccccccaac cgcccgtaga gattcggaat gatcaccata tagccttcac aggccagctg
     1141 ggcgccaaga tctttcattt gtccggtcaa tccccaccga tcatgcagca agacgacacc
     1201 tggataggtt cctgcttgtt gcggccagaa gaggaggcaa tcgacttggt gttccttcgg
     1261 caccttgctc ttgaagtagg ggtccaccat ttgatcagta tgggtgggga tcggcacacc
     1321 gcttgggaag cgggctgttc ccgtcccgat ctgcgcgagt gtgaacggtg ccgtttgtgt
     1381 cgtgggcatg gccgaaatgc tctaccgaag aagctttgag cgttgcataa ctttacttta
     1441 ctatatggag ccacttgcga gaatgtcaaa tgaggtccgc gaggcgacga ataaggagcc
     1501 tcacgtttgc ggacgggcgc gagttagtga gcgcccagtg tcttcatttg tggatgccgc
     1561 cgatccggca gtgtccgaag ctggtggact tttcaagatt cggagtttga ggtgtcgtga
     1621 acaaagattg tattggtgtg ggactgatcg gcgtgggtcg acatgggatg cgctatgccc
     1681 agcatcttat tcaggatctt cccacggcct ctctcagggc tgtgtgtcgg cgacatcctg
     1741 aacaggtctt tcatgtaccg ggtgccgagt cggtcaaggt ctacggcgac gccccatccc
     1801 tcatcgctga tccgacggtc gatgttgtcg tcgtggtcac tcctcccatt tactcatctg
     1861 aaatctgtcg actagcagcc caggctcgta aacctctgtt gatcgagaag ccattggcga
     1921 ctacggcaac tgatgcccgt atgatggtag ctcaggcttg cgaagccggg gttcccttga
     1981 tgacggcgca aaccctcagg ttcgatcata cgattcagca catgaagacg ctccaacctt
     2041 atatcggacg ttcccaacaa ctggatttgg tgttcaacat tgagatacga aaaacagctc
     2101 ccgaccatgc cgacggctat ggcaaacggg gtgcgttgct ggagatcggt gtgcacatgc
     2161 tcgacctggt tcgatttcta accggtgaag aagtac
//
LOCUS       DASTWE010000124         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_261762, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000124 DASTWE010000000
VERSION     DASTWE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261762"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFT30_01390"
     CDS             complement(<1..826)
                     /locus_tag="VFT30_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5091306.1"
                     /translation="MIIRGMRSLFPMVGIGVFILSLCPSALAMSETADNGGQKSEVEK
                     DLLIPDLLDSPPEPEDRLVILPEIKREGERFFLSSFKLPDKITFAGVPVPLDNWQVRE
                     RIEYEFYQFLEDQGESIILAKRTGRCFPPAEKQLAEAGLPDDLKYMLLVESKCISAAY
                     SRAKASGPWQFIPSTGRRYRLKSDAVRDERRNLEMSTEAAVKYLKYLKDFQDNDWFLA
                     MASYNAGEERIRKLLKEQKIADFWKMHGPRETMRYVPRIIAAKEIYSQPEKYLGLTK"
     gene            944..>1519
                     /locus_tag="VFT30_01395"
     CDS             944..>1519
                     /locus_tag="VFT30_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5091307.1"
                     /translation="MQALTLLNKTITDCTACPRLVVYRQAIARQKRKQYRDWTYWGRP
                     VPGFGDRQARLYVLGLAPAAHGGTRTGRVFTGDRSGDWLYDALYRYGFANQAASQHRN
                     DGLSLTDCYIGATVRCAPPGNKPAPDEFERCGRFLQDEVCLLKNHRVVIALGKIAFDH
                     YLKTCRTQGRAMPAPIPKFGHGVVYRLSWGVT"
BASE COUNT          339 a          407 c          404 g          369 t
ORIGIN      
        1 tcttggtcag cccaagatac ttctccggct gggagtagat ttccttagcg gcgatgatgc
       61 gaggaacata tcgcatggtc tcgcgtgggc catgcatctt ccaaaagtcg gcgatttttt
      121 gctccttgag cagcttgcgg attcgttcct caccggcatt ataggaagcc atggccaaga
      181 accaatcatt gtcctgaaaa tctttgaggt acttgagata cttcactgcc gcttcagtcg
      241 acatttcaag atttcgacgt tcatcacgga cggcatcgct cttcagtctg taccggcgcc
      301 cggtggaggg gatgaattgc catggaccgg acgccttcgc tctcgagtag gcggctgaaa
      361 tgcacttgct ctcgaccagc aacatatact tgagatcatc cggcaaaccc gcttcggcca
      421 attgcttttc agcaggaggg aaacaacgcc ccgtacgttt ggcgaggatg atgctttcac
      481 cttgatcttc caaaaattga tagaactcgt attcgatccg ttctctcact tgccagttat
      541 ccagcggaac cggcactccc gcaaacgtga tcttgtcggg cagcttgaat gagcttagaa
      601 agaaccgttc tccttcgcgt ttgatttccg gcaaaatgac caggcggtct tccggttcgg
      661 gtggcgaatc caataggtcc ggtatgagca aatccttttc cacttctgat ttttgaccgc
      721 cattatccgc tgtttccgac atggccaggg ccgaaggaca cagtgacagg ataaagacac
      781 cgattccgac cataggaaac aaggaccgca taccacggat gatcattctc gcactcctcc
      841 attacattgg acaaagccgt cacaaaacgc ggggttatgc tcacagctca ctcattcacc
      901 agcaagcaga atgggttccc gttcgtgtta cactgaggcc catatgcaag cgctgacact
      961 cctgaacaag actatcaccg attgcacggc ctgtcctcga ttagtcgtct atcgacaggc
     1021 gattgcacgg cagaaacgga aacaatatcg tgattggacc tactgggggc ggccggttcc
     1081 cggcttcgga gatcgccaag ctcggctcta tgttcttggg ctggctcctg ccgcacatgg
     1141 tgggactcga acgggacgtg ttttcaccgg agatcggagt ggggattggt tgtatgacgc
     1201 gttgtatcgg tatggattcg ccaaccaggc tgcttcgcag cacagaaacg atggcctatc
     1261 gttgacggat tgttacatcg gagcaaccgt gcgatgcgca ccgccgggga acaagcccgc
     1321 gccggatgag tttgaacgct gtggccgatt cttgcaagat gaggtttgtc tcctgaagaa
     1381 ccatcgcgtg gtgattgctt tgggaaaaat tgctttcgat cattacctca agacctgtcg
     1441 gacgcagggg cgtgctatgc cggcgccgat cccaaaattc gggcatggtg tcgtctatcg
     1501 cttatcctgg ggcgtgaca
//
LOCUS       DASTWE010000125         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1263520, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000125 DASTWE010000000
VERSION     DASTWE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263520"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..525)
                     /locus_tag="VFT30_01400"
     CDS             complement(157..525)
                     /locus_tag="VFT30_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091308.1"
                     /translation="MPWIGTSSTGQFACANASQRTLKDLRIKRKGQPVFVLGHVLARK
                     AQEATFEAFNDRLAVVKFSDEGLVGYDPRELLLPTEIDEHGVPYFEIRPCRSCGMLFP
                     LTMEERESDHEPEQCPDCTA"
     gene            615..965
                     /gene="arsC"
                     /locus_tag="VFT30_01405"
     CDS             615..965
                     /gene="arsC"
                     /locus_tag="VFT30_01405"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEU5091309.1"
                     /translation="MANITIYHKPTCTTCRQAVQLLKDSGTPFTAINYYEHSFTKTQL
                     KTLLKKAGLSPKDVLRTKEDLYHELGLAKKQLSDDELLDLMVKHPDLIQRPIVEKGDQ
                     AILARPADSIKKLL"
     gene            complement(1097..>1317)
                     /locus_tag="VFT30_01410"
     CDS             complement(1097..>1317)
                     /locus_tag="VFT30_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091310.1"
                     /translation="IAPTILATTADLQELVDAKTKRSTPDLPHMKGRRRIVVGELLLD
                     ALDGKVTFTVDQQTRTIRWSLSEQTTDR"
BASE COUNT          316 a          377 c          336 g          288 t
ORIGIN      
        1 caaacgtgac gtcattattc ttcgcgtcgc ggacattcct aagaccccct ggagaagcgc
       61 gtcttggcgc tccgtggtcg ggcgggtgag atcaacggtc tttttgaaat accctgtagg
      121 ctatgcttat cgataggttg ctgaagacca gactcatcag gcggtacagt cggggcattg
      181 ctccggttcg tggtcagact cgcgttcttc catggtaagc ggaaagagca ttccacagga
      241 tcgacaaggc cgaatctcaa aatacggaac gccatgctca tcgatttcag taggaagaag
      301 gagttctcga gggtcatagc ccacgagtcc ctcatcgctg aatttcacaa cggctaaccg
      361 gtcgttgaag gcttcaaagg ttgcttcctg cgctttgcga gcgagcacat gcccaaggac
      421 aaagaccggc tgacccttgc gcttgatacg aagatccttc agcgttcgtt gagaggcatt
      481 agcgcaggcg aactgaccgg tggagcttgt tccaatccac ggcataacga tcctgtttcc
      541 gacagagaac tggttgggat ctaacacaga ctgaaaaaaa tcgtcaagac gcgctttttc
      601 accaaggagt ccctatggca aacatcacga tctatcataa accaacctgc acgacatgcc
      661 gacaagccgt ccaactgctc aaagacagcg ggacgccttt tacagctatc aactattacg
      721 aacactcgtt taccaaaaca cagcttaaaa cgctcttgaa gaaggccggc ctatccccca
      781 aagacgtgct tcgaaccaag gaagacctgt accacgagct cggcctggcg aagaaacagc
      841 tctccgatga tgagctactc gacctgatgg tgaaacaccc agaccttatt caacggccga
      901 tcgtggaaaa aggcgaccag gccatcttgg cgagaccggc ggactcgatc aagaaactct
      961 tgtaggttgt ggccactgcc ggctcactaa gatacggccc gctcaccgct gacactgcgc
     1021 gctcaccgac tggcgcccgt gcgcaaactt gacgttcctt cttcgtcgcg tcgcgcacct
     1081 ctttattcat tgcgcctcag cgatcagtgg tctgctcgga gagcgaccac cgaatcgtcc
     1141 gtgtctgttg atcaacggtg aaggtgacct tcccgtctag cgcgtcaagc agaagttcac
     1201 ccaccacgat tcgtctcctt cccttcatgt gcgggagatc tggagtcgat cgcttcgtct
     1261 tggcatcgac taattcttga aggtcggccg tggtcgccaa gatggtcggc gcaatct
//
LOCUS       DASTWE010000126         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_197111, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000126 DASTWE010000000
VERSION     DASTWE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197111"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFT30_01415"
     CDS             complement(<1..405)
                     /locus_tag="VFT30_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5091311.1"
                     /translation="MAITDTYVVTVLLDVNETQDVDDRAESRSYIVADRNSGHAIIID
                     AVIENVERDLRVTKELGLTLLFVIETHIHADHITGASLIKERTGAQIVYGGGAKGVVT
                     GVDQFLFEDEALRFGNTVLTALATPGHTDGCTS"
     gene            complement(526..>1073)
                     /locus_tag="VFT30_01420"
     CDS             complement(526..>1073)
                     /locus_tag="VFT30_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5091312.1"
                     /translation="RAGVRYSRLFHLLSSVIRLCFGVRWRIATVSAVPFLVCETSSSL
                     TYSVIIFEMGRRYDHMDVRVRSLEEAREFYATLLPALGFTRQMDVKGWLSFEAADSGK
                     ATEFFGITESPHHVPNECRIAFWAENTSDVDRLAKIAVQAGARNVEGPGYDEGPGYYA
                     VFFEDPSGNRLEICHRDDEEK"
BASE COUNT          237 a          299 c          289 g          248 t
ORIGIN      
        1 gcttgtgcag ccgtccgtat gacccggagt cgcgagtgcc gtcaggaccg tgttgccgaa
       61 ccgcagcgcc tcatcctcga acagaaattg atccacgccg gtgactacgc ctttagcccc
      121 gccgccatac acgatttgcg ctccggttct ctctttaatg agcgatgccc cagtaatgtg
      181 gtcggcatgg atgtgcgttt caatgacgaa gaggagggtg agcccaagct ctttggtaac
      241 tcggaggtcg cgctcgacat tttcgatgac ggcatcgatg atgattgcat ggccagaatt
      301 gcgatcggcc acgatgtagg atcgactttc cgctctgtca tcgacatcct gcgtctcatt
      361 gacatccaac aatacggtca cgacgtaggt atcagtgatt gccatggcat actctcttcg
      421 gaagatctgt gtgtggcgaa tgacgaacgg cggccattct aacttgtcag ttcggcttgg
      481 tctatcgcag tgtcaggtga tgtggcggga agtgcagtct ctaccttact tttcttcgtc
      541 gtctcggtgg cagatctcga gacgattgcc gcttggatct tcgaagaaaa ccgcataata
      601 cccgggacct tcgtcatagc cgggcccttc gacatttcga gcgccggcct ggacggcgat
      661 tttggcgagc cggtccacgt cactggtgtt ttccgcccag aacgcaatcc ggcactcatt
      721 cggaacatga tgcggggatt ctgtgatgcc aaagaattcc gtcgccttcc cggaatcggc
      781 tgcttcaaac gacagccaac ctttaacatc catctggcgt gtgaatccca gtgccggcag
      841 gagggtcgca taaaattcgc gcgcctcctc caggctgcgt acccgcacat ccatatgatc
      901 gtatcgtcgt cccatttcaa aaattataac agaataggtg agcgaggacg aagtctcgca
      961 aacaagaaaa gggactgccg agactgttgc gatgcgccat cgcacgccga agcagagccg
     1021 gatgacgctt gacagcagat gaaataatct cgaatatctc acgccagcgc gta
//
LOCUS       DASTWE010000127         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_584855, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000127 DASTWE010000000
VERSION     DASTWE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584855"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..>1106
                     /locus_tag="VFT30_01425"
     CDS             94..>1106
                     /locus_tag="VFT30_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5091313.1"
                     /translation="MITPEVISAHNLTQDEYERIKQLLDREPTLDELGIFSVMWSEHC
                     SYKSSRVHLKRLPTTGERVIVPPGENAGVVDLGDGWCAAFKIESHNHPSFIEPFQGAA
                     TGVGGILRDIFTMGARPVASMNSLRFGSLEHPKYGRRNRSLLAGVVDGIAHYGNAFGV
                     ATVGGEVAFDDAYAFNPLVNAFALGLVRHDQIFFGKATGIGNPVLYVGAKTGRDGIHG
                     ATMASAEFDEEALEKRPTVQVGDPFLEKLLLEACLEAMRSGAIAGIQDMGAAGLTSSS
                     CEMAARAGTGIELDLTLVPQREVGMTAYEMLLSESQERMLIVAHSGREREVMDIFRKW
                     DLDA"
BASE COUNT          254 a          265 c          307 g          280 t
ORIGIN      
        1 cttgggcgca ggtcctctga ttcctgtggg acatcaatac caaacaccaa tgtcctaaga
       61 cctacagacc gaaagaccat tttgcacttg tcaatgatca ctcctgaagt tatttccgca
      121 cataacctta cgcaagacga atacgaacga attaagcaac tccttgaccg tgaaccaact
      181 ctggatgaac tgggaatctt ctcagtcatg tggtcggaac attgctcata taaatcatct
      241 cgggtacatc tgaaacgact cccaaccact ggcgaacgag taattgttcc acctggtgag
      301 aatgccggcg tcgtggacct tggcgacggc tggtgtgcgg ctttcaaaat tgaatcgcat
      361 aatcaccctt cattcataga gccttttcaa ggtgcggcaa cgggtgtcgg aggcatactc
      421 cgcgacattt ttacgatggg cgctcgcccg gttgcctcaa tgaatagctt gcggtttgga
      481 tcgctcgaac atccgaagta tggaagacgg aatcgttcgc ttctggccgg agtggtagat
      541 ggaattgctc attatggaaa tgcttttggc gtagcaacgg ttggcggtga agtcgctttc
      601 gatgacgctt acgcatttaa tccgctcgtg aacgcctttg cgcttggtct tgtgcggcat
      661 gaccagatct tttttggaaa ggctacggga attggcaatc ctgttttgta cgttggagca
      721 aagactggcc gtgatggaat tcatggcgcg acgatggcgt cagcagagtt cgatgaagag
      781 gctcttgaga agcgaccaac tgtccaggtt ggtgatccgt tcctggagaa actcttgttg
      841 gaagcttgcc tcgaagcaat gcgaagtggc gcaattgcag gcattcaaga catgggcgcc
      901 gctggtctga cttcgtcttc atgcgagatg gctgctcgcg cggggaccgg aatcgagctc
      961 gatctgactt tagtccccca acgtgaggtt ggaatgactg cttatgagat gttgttgtcg
     1021 gaatctcagg agcgcatgct gatcgttgcg cactcgggtc gtgagcgcga agtaatggac
     1081 atcttccgca agtgggacct cgacgc
//
LOCUS       DASTWE010000128         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_649721, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000128 DASTWE010000000
VERSION     DASTWE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649721"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFT30_01430"
     CDS             complement(<1..269)
                     /locus_tag="VFT30_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091314.1"
                     /translation="MIALVLAMVTLLPMTALAEPIQDSSALNAQSTRTAPLTIGEVLA
                     RIELTHPLLRAMGAERSKARAKILKALGAWEPQIKNYTQTERYNT"
     gene            412..1311
                     /gene="nhaR"
                     /locus_tag="VFT30_01435"
     CDS             412..1311
                     /gene="nhaR"
                     /locus_tag="VFT30_01435"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEU5091315.1"
                     /translation="MDWLNYHHLLYFWSVAKHGTVTKACEELRLAQPTISGQIHLLEN
                     TLGEKLFIRTGRRLALTEMGQLVFKYAEDIFATGQELMNTVKGQGNGHPTRLVVGIAD
                     AVPKPLATQLLKSALKLYRPTRLICNEDKLRELLTDLAAHRSDLVIADTPAPPSVKEH
                     TYNHPLGESGVTLFATAKLASQYRRGFPQSLRDAPLLLPTSSSMLRRLLDQWLVNHGM
                     QPNIVGEFDDSATLKAFGQDGHGIFPGASVIEKEICRQYRVQVVGQVEGLKQHFYAIT
                     VERRLKHPIVLALVRTARRELLS"
     gene            complement(1342..>1686)
                     /locus_tag="VFT30_01440"
     CDS             complement(1342..>1686)
                     /locus_tag="VFT30_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine synthase F"
                     /protein_id="HEU5091316.1"
                     /translation="RAEFGHEREYLVQVDRPFDQSFLDHLSRGVVILGSKTKPCRTMR
                     MERDQFRIILTEGRNRQIRRMCQALGYRVITLHRTRIMHITVNGLSAGNWKELTSPER
                     EELLRAVGRPQV"
BASE COUNT          367 a          497 c          460 g          362 t
ORIGIN      
        1 cacgtgttat aacgttcggt ctgtgtatag ttcttaatct gcggttccca ggccccgagc
       61 gccttcagga tcttcgctcg agccttcgat cgctcggctc ccatcgcccg aagcagagga
      121 tgcgtcaatt cgatgcgagc caacacttct ccgatggtca ggggagcggt gcgcgtcgac
      181 tgagcgttca atgcgctgga atcttgaatc ggttccgcca gtgcggtcat tggcaagaga
      241 gtcaccatgg ccaataccag agcgatcatg agggatgctc ctttcacatc gggtggcgga
      301 cggcgcccac actagcaatc tctcgttcat ttttcaaaat agataaaacg gattggagtt
      361 atcggctaat ccgatgtata gtgtcttccc taatttgagg cctagggcca tatggattgg
      421 ctgaactatc accacctgct gtatttctgg tccgtcgcca aacatggaac ggtgacgaaa
      481 gcctgtgaag aactccgcct ggcccagccc acgatcagcg ggcagatcca cctcttggaa
      541 aatacgttgg gagaaaagtt attcatacga accggccgcc gcctggcgct gacggagatg
      601 ggccagctcg tgttcaagta cgcggaggat atctttgcga cggggcaaga gctgatgaac
      661 accgtgaagg ggcagggcaa tggacatccg acgcggctgg tcgtcggcat tgcggacgcc
      721 gtgccgaaac cgctggcaac ccagctcctc aaatcggctt tgaagctgta tcggccgacg
      781 cgtttgatct gcaacgaaga taaattgcgc gaactcctga ccgatctggc cgcccatcgg
      841 tcggatctgg tgatcgctga cacaccggcc ccgccgagcg tcaaggagca cacgtacaac
      901 caccccttgg gcgaatcggg agtcacgctg tttgccacgg cgaagctggc ctcgcagtat
      961 cgccgaggtt ttccccaatc gcttcgcgat gcaccattac tccttcctac ctccagctcc
     1021 atgcttcggc ggctgttgga ccaatggctg gtcaaccacg gaatgcaacc caacattgtg
     1081 ggagagttcg acgacagcgc gacattgaaa gcgttcggac aggacggaca tggaatcttc
     1141 cccggcgcgt cggtgatcga gaaggaaatc tgccgtcaat atcgggtgca ggtagtcggc
     1201 caagtagaag ggctcaagca gcacttctat gccatcaccg tggagcggcg gctgaagcac
     1261 ccgatcgttc tcgccctcgt ccgaaccgcc cgtcgcgagt tgctcagtta gccgtcaatg
     1321 ggcttccact agaaaaatgc gtcagacctg aggccgccct accgctctca ggagctcctc
     1381 ccgctccgga ctcgtgagtt ctttccaatt cccggcactc agcccgttga cagtaatgtg
     1441 catgatcctc gttcggtgca atgtaatcac tcgatagccc agcgcctggc acatacgcct
     1501 gatctgtcgg ttccgtccct ccgtaagaat gatgcgaaac tgatcacgct ccatgcgcat
     1561 cgtcctacaa ggctttgttt tgcttccaag aataaccaca ccccgcgaca gatgatccaa
     1621 aaaggattgg tcgaacggac gatcgacctg cacgagatac tctcgttcgt gtccgaattc
     1681 ggctcg
//
LOCUS       DASTWE010000129         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1069808, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000129 DASTWE010000000
VERSION     DASTWE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069808"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..>1147
                     /locus_tag="VFT30_01445"
     CDS             140..>1147
                     /locus_tag="VFT30_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HEU5091317.1"
                     /translation="MTIHTFAGKPAPHAILVDVEKLLSAYWTEKPDASVREQRVSFGT
                     SGHRGSSFRRSFNENHIAAITQAICEYRTAQHTTGPLYLGKDTHALSVPAFATAIEVF
                     ATNGVDVMIDQNNGYTPTPVISHAILTYNRGRTSGLADGVVITPSHNPPEDGGIKYNP
                     PSGGPADTQITKWIEDRANAFLAANLQGVKRLPIEQARRASTTHRHDYIGAYVSDLTN
                     VVDMDAIKSAKLKLGIDPLGGSGVAYWQPIVERYGLNLEIVNPAVDPTFRFMPLDWDG
                     KIRMDCSSPYAMANLIALKDRFDVAFGNDADNDRHGIVTRCGLMNPNHYLAVSIAYLF
                     AN"
BASE COUNT          284 a          323 c          277 g          263 t
ORIGIN      
        1 gttctataag gggtttctcc cccatcttca cgcaagatgc aaaaagcctc gacgtaccgg
       61 acagaaggaa agtcctcttt cgacttacgt tgtccctctg cagttgacag tagcctgaca
      121 ggcgaactac acctttacta tgactattca tacttttgcc ggcaaaccag cacctcacgc
      181 catcctcgtg gatgtagaaa aacttttgtc cgcttattgg actgaaaaac cggatgcctc
      241 tgtccgagaa caacgcgtat cattcggtac ttccggacat cgtggctctt cattcagacg
      301 aagcttcaat gagaatcata tcgcggccat tactcaggca atttgcgaat accggactgc
      361 acagcatacg accggacctc tctatttggg aaaagatacg cacgcactgt ctgtgccggc
      421 cttcgcgact gcgattgaag tcttcgcaac gaatggcgtt gatgtcatga tcgaccagaa
      481 taatggatac acaccgacac cggtcatctc gcatgcaatc ctgacctata accgaggccg
      541 aacctcaggt ctggccgatg gggttgtcat cactccatcc cacaatcccc cggaagacgg
      601 cggtatcaag tacaacccgc cgagcggggg accagccgat acacagatca cgaaatggat
      661 cgaggaccga gccaatgcct ttctagccgc caacctccaa ggagtgaaac gtcttccgat
      721 tgaacaggct cgacgggcat caaccacaca tcggcacgac tacattggag cctacgtgag
      781 cgatctgacc aacgtggtcg atatggatgc catcaagtcc gcgaagctga agcttgggat
      841 cgacccactt ggggggtccg gtgtagccta ttggcaaccg attgttgagc gttacggact
      901 caatcttgag attgtgaatc cagcagtcga tccaacgttc cgcttcatgc ccttggattg
      961 ggacggtaag attcgcatgg actgttcgtc tccctatgcc atggcaaatc tcatcgcgct
     1021 caaagatagg ttcgatgtgg cctttgggaa tgatgcggac aatgaccgac atggaatcgt
     1081 cactcgttgc ggtttaatga atccgaacca ttacctggct gtctcgatcg cctacctctt
     1141 cgccaac
//
LOCUS       DASTWE010000130         1240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_681908, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000130 DASTWE010000000
VERSION     DASTWE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681908"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..>1240
                     /locus_tag="VFT30_01450"
     CDS             261..>1240
                     /locus_tag="VFT30_01450"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5091318.1"
                     /translation="MVSMYSTTYVDDIREIKEENPFESMMSRFDRAAQLLDLDPDLYA
                     VLRVPNRELKVYIPTKMDSGRIQVFEGFRVQHNFARGPAKGGIRYAPDVNIDEVRALA
                     AWMTWKCAVVNVPFGGAKGGIICDPQQMSMGELERMTRRYAAELLDFIGPEKDVPAPD
                     MNTNEQTMAWIMDTYSMHARHTVNAVVTGKPIDLGGSAGRREATGRGILIVVNEAIKR
                     FNMTPSETRVVVQGSGNVGGIGAQLLHETGYKVVALSDIHGGIYNQNGIDIPKALAYL
                     RTTRSFEGYEGVEFVNNQELLELECDVLVPAATENQINSQNADKIKCKVLA"
BASE COUNT          304 a          301 c          363 g          272 t
ORIGIN      
        1 gccatcacga tcagcttgtg cgaatcgaga tctccccggc tgagatggac cgtgtgttac
       61 ggaaagaatt gattgatgag cttgcgagac gctttcgtga gcttggattt aaatatgtga
      121 cgctcgatct cgagggtttt agaagcggat cgatgaatga agttttagat taatcatgtg
      181 aagcctgtaa atcccgtcat cctgtgaatt gttagacagg attacaagat ttacaggatt
      241 cacaggatgg agagaatccg atggttagca tgtacagcac tacatacgtt gatgatattc
      301 gcgagataaa agaagagaac ccgttcgagt cgatgatgtc gcgattcgat cgcgccgcac
      361 agttactgga tctcgatccg gacctctacg ccgtgttgcg agttcccaat cgcgagctga
      421 aggtctacat ccccacaaag atggattcag gccgtattca ggtgtttgaa ggtttccgcg
      481 tccagcataa cttcgcgcgt ggtccagcca agggcggtat acgctatgcg cctgacgtca
      541 atatcgacga agtgcgcgcc ctcgccgcgt ggatgacgtg gaagtgtgct gtggtgaatg
      601 tgcctttcgg tggcgccaag ggcggcatca tctgcgatcc gcagcagatg tccatgggtg
      661 agctcgaacg catgacgcgt cgctacgctg ccgagctgct cgacttcatc ggaccggaaa
      721 aggacgtgcc cgctccggac atgaatacca acgagcagac aatggcgtgg attatggaca
      781 cctactcgat gcacgcacgc cacactgtca acgcagtcgt gaccggtaag ccaatcgatc
      841 tcgggggatc ggcggggagg cgtgaggcga caggccgcgg catactaatt gtagtcaacg
      901 aagccatcaa gcgattcaat atgacgccgt ccgagacccg tgtggtagtg cagggctcag
      961 gaaatgttgg cggcattggt gcgcaactac tccacgagac cggttacaag gttgtagcgt
     1021 tatcggacat ccatggagga atttacaacc agaacgggat cgatattccg aaggcactgg
     1081 cttacttgag aactacgcgg tcgttcgaag gctacgaggg tgtggagttt gtcaacaacc
     1141 aggagctgct cgagctcgaa tgcgacgtgc tcgtcccggc ggcaactgag aaccagataa
     1201 actctcagaa tgccgataag atcaagtgca aggttctcgc
//
LOCUS       DASTWE010000131         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1102148, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000131 DASTWE010000000
VERSION     DASTWE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102148"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFT30_01455"
     CDS             complement(<1..297)
                     /locus_tag="VFT30_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEU5091319.1"
                     /translation="MGKTLIIAEKPSVAADIARVLGGFTKHADYFESDQYVLSSAVGH
                     LLELVVPEQYEVKRGKWSFANLPIIPPHFDLNPIEKTEARLKLLTKLIKRKDVDS"
     gene            complement(515..>1042)
                     /locus_tag="VFT30_01460"
     CDS             complement(515..>1042)
                     /locus_tag="VFT30_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU5091320.1"
                     /translation="LAQQGGLISEFPLGTPAIASNFPRRNRIISGMSRGCLVVEAALQ
                     SGSLITARQALEQGREVFAVPGSIHSPLAKGCHSLIKQGAKLVESAEDILDELGYPAP
                     IPEGIDNTGETEREKPEREKSLLLRHLGHEIIDIDTLSVRSGLTVETVSAMLLTFELD
                     GIVASLPGGGYQRLA"
BASE COUNT          239 a          275 c          277 g          251 t
ORIGIN      
        1 ggaatccaca tccttgcgct tgatgagttt ggttaacagc ttaagccgtg cctcggtttt
       61 ctcgatggga ttgaggtcga aatgcggcgg gatgatgggt aagttggcaa aactccattt
      121 cccccgtttg acttcgtatt gttccggcac gacaagttca agcaaatggc cgacagccga
      181 agaaagcaca tactgatcgc tttcaaaata atcggcatgc ttggtgaacc cgccaaggac
      241 ccgcgcaatg tcggcagcga cggaaggctt ctcggcgata attaaggttt tacccatgaa
      301 gcttccatga atgtccgcag gttggcggca gcgggttaaa taacgggtgt agatgactga
      361 tcattatcag tacaataaca gaaaattgtt ggatggccgg caagttggtc ggggatcgta
      421 gtctcgctga cagtgaatgt ctgtggcaat cggtcggcat accctatcat ttggcttgcg
      481 acttcagaca ccatgggacg ttcatcaata ggacctatgc aagcctttgg tagccgccac
      541 ccgggaggct tgccactatg ccgtccaatt cgaacgtcaa tagcatcgct gataccgttt
      601 ccaccgtcaa gccgctacgg acggagagcg tatcgatatc gatgatttca tgaccgaggt
      661 gtctcaacag cagagatttt tctctctccg gtttttctct ttccgtttcg cccgtattat
      721 caatgccctc gggtatcggg gccgggtagc ctaattcatc caaaatatcc tccgcacttt
      781 cgacgagttt cgctccctgc ttgatgagcg agtggcaacc cttcgcaaga ggagaatgga
      841 tggaacccgg gacagcaaat acctcccgcc cctgttccag agcctgcctt gcggtaatga
      901 gagacccgct ctgcagcgca gcctcgacca cgagacagcc tcggctcatg ccgctgatga
      961 tgcggttgcg gcgcggaaaa ttgctcgcta tagccggcgt gcccaacggg aattcggaaa
     1021 taagtcctcc ctgttgagct aa
//
LOCUS       DASTWE010000132         3073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_197204, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000132 DASTWE010000000
VERSION     DASTWE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197204"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="VFT30_01465"
     CDS             complement(<1..1889)
                     /locus_tag="VFT30_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU5091321.1"
                     /translation="MFPSLAAAFSLGLLCGAQISFFPLSVIVLLAGIAVGSSMLERAG
                     YIDSPSALLLYFSLLSGVVYWSFTTPTSESPQLSPPLHETVLASIVGRVMAPVQHSVG
                     RQTILVQAEESDSESRRIRLVWRDPGITLHHGDRIAFRGKLHRPRGSLNPGGFDYAAY
                     MERHGIDFLTTVTGAQAVMLLDTETPIGLWSLWSRIDHWRATIRAAAIKTLSQPAQGL
                     FLGMIIGDRGYLGQELQDWFMVTGTVHLLSISGSHLGLVAIVVFWLVKRLVLGLPLTL
                     LLTLSRTVTPSKIAILLAWPAVALYAFLAGAELATMRSLVMITLAMIAMWLGYERHLG
                     HAMAVALLAILSHDPRAIFDISFQLSFLSVLVMVRMIVLTKSWNADGVGADGQLRHRF
                     ASHALKALSMSGAVTVATLPMVAFYFNQVPWLGVITNLIAVPFTGIILVPLGLLAALW
                     TIITETESLILGSALEQLFGLMVLGLQWCARFPGGEWHIAAPSIPTIALFYSGLLMAS
                     FQAIPWRVRVAGAGLSILLIGWWLSPPLSKADGDHWRVTFLDVGQGDSAVVELPDGQT
                     VLIDGGARYDRFDMGKNVVAPFLWSRRIHHITHVIGTHQQLDHVGGLIWVLRHMSVGR
                     YWGGG"
     gene            complement(1925..>3073)
                     /gene="glmM"
                     /locus_tag="VFT30_01470"
     CDS             complement(1925..>3073)
                     /gene="glmM"
                     /locus_tag="VFT30_01470"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5091322.1"
                     /translation="MGVDVLLVGPMPTPAIAFLTRSLRADAGVVISASHNPYQDNGIK
                     FFSSDGFKLPDEVEARIEELIISNEISHLRPTADLIGKAYRIDDAEGRYIEFAKRSLP
                     KDLDFQGIKLVVDCANGAAYKLAPTVLRELGATVEVIGNKPDGMNINAGCGAVHPELL
                     QESVRRYNADLGIALDGDADRAVFVCEQGTVIDGDHVMAALGLDLHRNGLLAKQTLVG
                     TVMSNFGLELSLSKAGVKLIRTPVGDRYLLERMLAEGYNFGGEQSGHFIFLDHNTTGD
                     GLISALQMLSLVKRTKKPLSELAKAMTAVPQVLLNVHVTKKPRLESIPDIDCAIQESE
                     RRLDGCGRVLIRYSGTEPLLRIMVEGEQSTKVKEVAEDLARVVRKHIG"
BASE COUNT          787 a          886 c          780 g          620 t
ORIGIN      
        1 atcccaccac cccagtaccg tccgactgac atatgccgga gtacccaaat cagcccgccg
       61 acgtgatcca gctgctggtg cgtcccaatg acatgagtaa tatggtggat tctacggctc
      121 cacagaaacg gtgcgaccac atttttcccc atgtcaaatc ggtcatatcg agctccgccg
      181 tcgatcagaa ctgtctgacc atccggcaac tcaacaacgg cactatctcc ttgtccgaca
      241 tcaaggaatg tgacacgcca atgatcaccg tctgctttcg acaggggagg actcagccac
      301 caaccgatca acaatatcga cagaccggca ccagcaactc ggacgcgcca cggtattgcc
      361 tgaaaacttg ccataagtaa cccagagtaa aacagcgcga tcgtaggaat ggatggtgca
      421 gctatatgcc actccccgcc tggaaaacgg gcacaccact gcaatccaag aaccatcaac
      481 ccgaataatt gctccaacgc cgagccgagg atcaaagact cggtttccgt aataatcgtc
      541 cacagggccg ctagcagtcc caaaggtacc aagataatac cggtaaacgg gacggctatc
      601 aggttcgtga tgactcccaa ccacgggacc tgattgaaat agaatgcaac catgggcaaa
      661 gtggccacgg tcacagcccc gctcatcgac agcgctttga gcgcatggct cgcgaagcgg
      721 tgtctcagct gaccgtcagc ccctacaccg tcggcgttcc atgatttcgt gagtacgatc
      781 attctgacca tcacgagcac agaaagaaaa gagagttgaa aggaaatatc aaagatggcg
      841 cgaggatcgt gcgagaggat cgctaggaga gccacagcca tcgcatgccc cagatggcgt
      901 tcatacccta accacattgc gatcatcgcc agagtgatca tcacgagcga acgcatggtc
      961 gctaactcgg cccccgcaag aaacgcatag agtgcgacag cgggccaggc aagcagaatt
     1021 gcaatcttcg aaggcgtgac tgttcgtgat agcgtcaaca agagagtgag gggaagccca
     1081 agaacgagtc tttttaccaa ccaaaaaacc acgatggcca cgagcccgag atgtgaaccg
     1141 gagatcgaga gcagatgaac ggttccggtc accatgaacc agtcttggag ctcttgtccg
     1201 agatagcctc gatccccgat aatcatgccg agaaatagcc cttgtgcggg ttggctcaat
     1261 gtcttgatgg ctgccgcacg tatcgtggct ctccaatgat cgatccggct ccataaactc
     1321 cacaacccga ttggagtctc cgtatctaac aacatcacag cctgagcacc ggttaccgtg
     1381 gtcagaaagt cgatgccatg acgttccata taggcggcat agtcgaatcc accagggttt
     1441 aacgacccgc gtggacggtg aagttttccc cgaaacgcaa tacggtcgcc gtggtgaagc
     1501 gtgataccag gatcgcgcca cacaagacgt atccgtctcg attctgaatc agattcctct
     1561 gcttgaacca agatcgtctg gcgtcccaca ctgtgctgaa cgggagccat aacccgaccg
     1621 acaatcgagg ctagaacagt ttcatgaagc ggtggtgaca gctggggaga ctccgatgta
     1681 ggggtggtga aactccagta aactactccg gagagaaggc tgaaatacag cagcagtgcg
     1741 gagggtgagt cgatatagcc ggctcgctcg agcatgctgg atccaacggc gatgccggcc
     1801 aacagaacta tgacggagag cgggaaaaag gagatctgtg ccccacacag aagaccgagg
     1861 gaaaacgctg ctgcgaggga cgggaacatg agtttccctc atgtaccggt ggaggaggga
     1921 acactcagcc gatatgcttt cgtactaccc tggcaaggtc ctcagccact tccttgacct
     1981 tggtggactg ttctccttcc accataatcc gcaacagcgg ctcagtccca gaatatctga
     2041 tgaggacccg cccacacccg tccaggcggc gttcgctctc ttgtatagcg caatcgatgt
     2101 cgggaatcga ttccaatcgc ggcttcttcg tgacatgcac attcaacaac acttgtggca
     2161 cagcggtcat tgccttggcc agctcggata acggcttctt ggttcgtttc accaatgaca
     2221 gcatctgcag agccgagatg aggccgtctc cggttgtatt gtgatcaagg aatatgaagt
     2281 gtcccgattg ctcccctccg aagttatagc cttcagccaa cattcgttcg agcagatacc
     2341 gatcaccgac cggcgtccga atcagcttga cgcccgcttt cgacaacgac agttccagtc
     2401 caaagttgct catcacggtt ccgaccaacg tctgcttggc gaggaggccg tttcgatgaa
     2461 gatccaggcc caaggctgcc atcacatgat caccatcgat gacggttcct tgctcacaga
     2521 caaagacggc tcgatcagca tcgccatcca aggcgatacc gagatccgcg ttgtaacggc
     2581 gcaccgactc ttgcaacagt tcaggatgga cggcaccgca acccgcattg atattcatcc
     2641 catccggttt attgccgatg acttcaaccg tggctcccaa ttccctgagg actgtcggag
     2701 ccagtttgta tgcagcacca ttcgcacaat cgacgacaag cttgatgcct tgaaaatcca
     2761 agtccttcgg caacgaccgc tttgcaaatt caatataacg cccctctgca tcgtcgatgc
     2821 ggtaggcttt gccgatgagg tctgctgtcg gtcgaagatg gctgatttcg ttcgaaatga
     2881 ttagttcttc aatgcgagct tctacctcat cagggagctt gaatccgtcg ctggaaaaga
     2941 atttaattcc gttgtcttgg taaggattgt gcgacgccga aatgaccacc ccagcatccg
     3001 cccgtagact cctggtcaaa aaggcgatcg ccggagtcgg catcggtccg actaacagca
     3061 catccacccc cat
//
LOCUS       DASTWE010000133         1291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1393116, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000133 DASTWE010000000
VERSION     DASTWE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393116"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..138
                     /locus_tag="VFT30_01475"
     CDS             7..138
                     /locus_tag="VFT30_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5091323.1"
                     /translation="MGRVYERQGKWTEAIDSYKQALVLNPDYALAKKALSRLISSLN"
     gene            150..536
                     /locus_tag="VFT30_01480"
     CDS             150..536
                     /locus_tag="VFT30_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5091324.1"
                     /translation="MGVTILVAVTDIFFYTKVRDALRPQGHTLERIRNHEEVRLKAAA
                     ANPAVMIINMNDVGVNAFQVLETLKSDPALGSFPILAFANHEEVETWKRAKELGVTKV
                     VSRNEFSSRTKDLVEELIHQQPSAHT"
     gene            567..>1291
                     /locus_tag="VFT30_01485"
     CDS             567..>1291
                     /locus_tag="VFT30_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HEU5091325.1"
                     /translation="MKQSPLYNHHIQLGATFEEVSGWSVPAHYGHVAVEHAAVRHAAG
                     LSDLSHRGKIRVTGDDRIKWLQSLISNDILTLQSGQGRYSSFLTHKGKMLGYFRVYIQ
                     PDVVWIEDVGEVGDATFQALRKFLLYGTKAKMENCMEGWGLLLVSGPKAAEIVSAAFS
                     TDVGTLELLHSVAATVGGQQALIVRTEETGEQDFALLLPVQGVPAAWEQLMASGAPFG
                     IKPVGAQAREALRIEAGLPKAGP"
BASE COUNT          302 a          362 c          359 g          268 t
ORIGIN      
        1 ctcaacctgg gacgcgtgta tgaacggcaa ggcaagtgga cagaggcgat cgacagttac
       61 aagcaggccc tggtcctcaa tcccgactat gccttggcaa aaaaggcgtt gagccgcctc
      121 atcagctcgc tcaattagga acgaattgta tgggtgtcac cattctcgtc gcagtcacgg
      181 acattttctt ttacaccaaa gttcgcgatg cgttacgccc gcaagggcac acgttggaac
      241 gtatccgcaa tcatgaggaa gtacggctga aggcggctgc ggccaatccg gcggtcatga
      301 tcatcaatat gaacgatgtg ggagtcaatg cctttcaggt tctggaaact ctcaaatccg
      361 atcctgcctt aggctcgttt cccattcttg cattcgctaa tcacgaagag gtggaaacct
      421 ggaaacgcgc caaggaactc ggcgtcacca aggttgtctc ccgcaacgaa ttttcgtcgc
      481 gcacgaaaga tctcgtcgaa gaactcatcc accaacagcc ttccgctcac acgtgaaggc
      541 cggtacataa ctcagacggc ataatcatga aacaatctcc tctttacaat caccacatac
      601 aattgggcgc tacattcgag gaggtctccg ggtggtcggt tccagctcac tatggtcacg
      661 tcgcggtgga acatgcggcc gtgcgtcacg cagcaggcct gtccgacctt tctcaccgag
      721 gcaagattcg ggtgaccggt gacgatcgga tcaagtggct gcagagcctc atcagcaacg
      781 acattctcac ccttcaatcg ggacaaggcc gctattccag cttcctcacc cataagggca
      841 aaatgctcgg ttactttcga gtctacattc aacccgacgt cgtgtggatt gaagatgtcg
      901 gcgaggtagg cgatgccacg ttccaagcgc tgcgaaaatt tctcctttac ggcaccaagg
      961 ctaagatgga gaattgcatg gaaggctggg gtttgctgct cgtcagcgga ccgaaagccg
     1021 cagaaatcgt ctcagcggca ttcagcaccg acgttggcac ccttgagctg ttgcacagcg
     1081 tcgccgccac cgtcggtgga cagcaggcct tgatcgttcg cacggaagaa acaggtgaac
     1141 aggactttgc cctgctgttg ccggtgcagg gagttcccgc tgcctgggaa cagctgatgg
     1201 caagcggcgc tccgttcggt atcaagcccg tcggcgcaca ggcacgggaa gcgttacgga
     1261 ttgaagcggg gttgccgaag gcgggaccgg a
//
LOCUS       DASTWE010000134         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_3595, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000134 DASTWE010000000
VERSION     DASTWE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3595"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFT30_01490"
     CDS             complement(<1..246)
                     /locus_tag="VFT30_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5091326.1"
                     /translation="MPVDGHLRVGAVGDLHCPRLSIGEIRSLFAQIAERSDVLLLCGD
                     ITDYGKPEEAQSFVTALPALNGIPVVAVLGNHDHESGE"
     gene            complement(267..578)
                     /locus_tag="VFT30_01495"
     CDS             complement(267..578)
                     /locus_tag="VFT30_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5091327.1"
                     /translation="MRRLAFVLTLSIVGGLAACQATTGKTASQTMNDASVTAAVQTKL
                     TTDRMANFTRVDVDTEQGIVQLSGVVQTSEQRARAEELTKQVNGVKRVHNNLQVQTVE
                     P"
     gene            complement(610..939)
                     /locus_tag="VFT30_01500"
     CDS             complement(610..939)
                     /locus_tag="VFT30_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5091328.1"
                     /translation="MRNGNYYDEGIGDATGWSAFMAGALIGAGVALLFAPQPGSELRG
                     LLRNYANRAKDDLMDKGQEAWDTAVERGKEYYNKGEEAIRDVGRSAKEFAKQGQDVVR
                     EPGRSAL"
BASE COUNT          211 a          311 c          228 g          259 t
ORIGIN      
        1 ttcgcccgat tcatgatcat ggtttcctaa tacggcgacg acaggaattc cgttcagagc
       61 cggaagcgca gtaacgaagc tctgcgcctc ctcgggcttc ccgtaatcag tgatgtcacc
      121 gcacaataag agaacgtcgg aacgctctgc tatctgcgcg aacaacgatc ggatctcacc
      181 gatgctaagc ctgggacaat gtagatcacc tacagctccg actctcaagt gtccgtcaac
      241 cggcatctcg tgtcactatt tccgtttcac ggttcaaccg tctgaacctg taaattattg
      301 tgaactcgtt tgacgccatt gacctgtttc gtgagctcct ccgctcgggc tctttgttcc
      361 gatgtttgca ccactccact gagttgcaca attccctgtt cggtatcaac gtcgactcgc
      421 gtaaaattgg ccatgcgatc ggtcgtgagc ttcgtttgca cagccgccgt aacggaagcg
      481 tcgttcatcg tctgggaagc cgtttttcct gtcgtggctt ggcatgccgc aagcccaccg
      541 actatgctta gcgtaaggac aaacgctagc cttcgcatga agcggcctcc gtgtgatgta
      601 tagtgaacgt tacaaagctg aacgccctgg ctcgcgaacc acgtcttgtc cctgtttggc
      661 aaattctttg gccgaacgtc ccacgtcgcg gatcgcctct tctcccttgt tataatattc
      721 cttgccccgt tccaccgcgg tatcccatgc ctcttgcccc ttatccatca gatcatcctt
      781 cgcgcgattg gcataattgc gcaacagacc gcgcagctct gagcctggct ggggagcaaa
      841 cagcagcgcg acgccggccc cgatcaacgc tccagccata aaagcggacc atcccgtcgc
      901 atccccaatt ccttcatcgt aatagtttcc gttgcgcata atatcgtcct ccttttgtag
      961 ctaaaaacct acaggttcta tgactacact cgagtttcat taatctgcc
//
LOCUS       DASTWE010000135         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_488207, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000135 DASTWE010000000
VERSION     DASTWE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488207"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFT30_01505"
     CDS             <1..371
                     /locus_tag="VFT30_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5091329.1"
                     /translation="DLMHADSEIPMVQAAVGGMAAMLEMTAKKLGMTTVLDQEGGLVG
                     VITDGDLRRFIQRGTDLVNTTAGELASHNPKTIGPNELAATAVEMMERYSITTLVVTE
                     DGRTIRGVIHLHDLLKNGIV"
     gene            387..983
                     /gene="pgsA"
                     /locus_tag="VFT30_01510"
     CDS             387..983
                     /gene="pgsA"
                     /locus_tag="VFT30_01510"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5091330.1"
                     /translation="MNRVPEVWRDIGQESLNLPNLLTLVRILLIPVFIILFVNPTPDQ
                     SLAAAIVFTVAAVTDMLDGYIARRTGQVTKLGKLLDPIADKLLVLSALILLMNVDRVS
                     ALVALLIVAREVAVTGIRAIAAGEGMIIPSETTGKYKMTLQVVAIILLILEGTGLAEL
                     GNMHLAGTGTLYLSLVLGYVSGSQYVWSFWKQVVAKGL"
     gene            1085..>1547
                     /locus_tag="VFT30_01515"
     CDS             1085..>1547
                     /locus_tag="VFT30_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091331.1"
                     /translation="MQLFRSACALFLLQIILIACSENEQPPQLAAFAYTNATGHCPVG
                     SRPGRGGATDEKVSADGIRYMVRTPSNYDATFAHPLLMVYAPAGQSRWAAERFTGLTT
                     AATGAGFVVAYVDHRQLSVSIIEQLGTIPDLIAKEWCIDEHRVYVTGHSDGG"
BASE COUNT          340 a          422 c          435 g          350 t
ORIGIN      
        1 aagatctcat gcatgccgat tcagagatcc cgatggtaca agccgccgtc gggggtatgg
       61 cggccatgct tgaaatgacg gccaaaaagc tcggcatgac gaccgtgctg gatcaggaag
      121 gcgggttggt tggagtaatt accgacggag acttacggcg ctttatccag cgaggcacgg
      181 atttggtcaa cacgacggca ggagaattgg cctcgcacaa tcctaaaacg atcggaccga
      241 acgaattggc tgcaacggca gtggagatga tggaacggta ttcgatcacc accctggtgg
      301 taacggaaga cggccggacg attcgtggcg tgattcattt gcatgactta ttaaaaaacg
      361 ggattgtcta gaggatagat gcagccatga accgcgttcc tgaagtctgg cgagacattg
      421 ggcaggaatc gctcaacctg cccaatctcc tgacgctcgt ccgcattctt ttgattcccg
      481 tctttatcat tctcttcgtc aatccaacgc ccgatcagtc cttggcggcg gccattgtct
      541 ttaccgtcgc ggctgtgacg gatatgttgg acggttacat cgcacggcga accggccagg
      601 tgaccaagct cggcaaactg ctcgatccga tcgcggacaa actcttggtc ttgtcggcgc
      661 tgattcttct catgaacgtg gatcgggtca gcgcgttggt cgcgctgctg atcgtcgcgc
      721 gagaagtcgc ggtgaccggc atccgtgcca ttgctgccgg agaagggatg attatcccct
      781 ctgagacgac cggcaagtat aagatgacgc tccaagtcgt cgccatcatt ctgctgatcc
      841 tggaagggac aggcctcgct gaactcggca atatgcacct ggccggcacc ggcaccttgt
      901 atctgtcact ggtgcttggt tatgtctccg ggagtcagta tgtgtggagc ttttggaagc
      961 aggttgtcgc gaaaggactc taacagatca gcgagacggt ctagttccag cgctcaacat
     1021 cgtacccact ttcaccttga agcggacaaa ctctcgataa tctcctcgcg acgtgatggg
     1081 caacgtgcaa cttttcagat ctgcctgcgc gttgttcctt cttcaaatca tccttatagc
     1141 ctgtagtgaa aacgagcaac caccacaact cgcagcattc gcctatacga acgcgactgg
     1201 gcattgtccc gtcggttcac gtccaggccg cggcggcgca accgatgaga aggtctctgc
     1261 tgatggcatt cgctatatgg ttcgcacacc ctccaactac gatgccacgt ttgcgcatcc
     1321 cctcttgatg gtctatgccc ctgccgggca gagtcgctgg gcggctgaaa gattcacagg
     1381 cctcacgacc gcagcgactg gtgccgggtt tgttgtggca tacgtcgatc accgacagtt
     1441 aagcgtctca atcatcgaac agctagggac aattcccgac ttgattgcaa aggagtggtg
     1501 catcgatgag cacagagtgt atgtcaccgg ccattcagac gggggca
//
LOCUS       DASTWE010000136         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_423551, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000136 DASTWE010000000
VERSION     DASTWE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423551"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFT30_01520"
     CDS             <1..301
                     /locus_tag="VFT30_01520"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEU5091332.1"
                     /translation="THPDGLNPMTITAMQEIGVEVYHHRSKHVEEFVGQSFTYVITVC
                     DRAKESCPVFPPAFKILHWSFEDPATIPLENRSEAFRRVRDEIAERIRQFLNEVV"
     gene            complement(584..1465)
                     /locus_tag="VFT30_01525"
     CDS             complement(584..1465)
                     /locus_tag="VFT30_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5091333.1"
                     /translation="MADRKKRLDSNVSGNFYVDATCINCDTCRQLAPLSFEEVGDYSA
                     VGCQPDSEPQIHQAYQALLACPVGSIGTEHSDKARLQTAMASFPLQIENGVSYCGFNS
                     EKSFGANSFFIEHPDGNWLIDSPRYIKHLVEAFEQRGGIAHIFLTHKDDVADADKYAA
                     HFGAKRIIHQADRDAAPTAERVIGGEETVRVGSEFQIIPVPGHTAGCMALLYRDRFLF
                     TGDHLWWDPHTKSLEAPTRLLWRKWTLIESIRKLLDYPFEWVLAGHGDRIHLPQSEMH
                     TALQDLIRRRTPTTVST"
     gene            complement(1470..2177)
                     /locus_tag="VFT30_01530"
     CDS             complement(1470..2177)
                     /locus_tag="VFT30_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEU5091334.1"
                     /translation="MAHGTDDPGRRRDRHVGNGDLAGVMHDESFDSTDYLSNADPVMR
                     RLIEEVGPFMLKPKIRRSPFESLARAIAYQQLHDKAAESILKRFIALFPGRRFPRPDE
                     LLAVNVRSIRKAGFSRAKVSALRDLALKTLDGTVPTGREVGILDDHAIIDRLIEVRGI
                     GRWTVEMFLIFQLGRPDVLPVDDFGLRNGFRIAYRRPLMPTPKQLLQYGERWKPHRTV
                     AAWYLWRAADRERQAYK"
     gene            complement(2129..>2429)
                     /locus_tag="VFT30_01535"
     CDS             complement(2129..>2429)
                     /locus_tag="VFT30_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5091335.1"
                     /translation="HLHFAGATAYLGSKAALEQYTKGLAQELAPKGITVNTVSPGFTE
                     TGMMTEEYRQIGIQLSPMKRLGVPKDIADVVAFIVSEEARWLTGQTIQAGGGIVM"
BASE COUNT          534 a          674 c          647 g          574 t
ORIGIN      
        1 gactcatccg gatgggctca acccgatgac gatcacagcg atgcaggaga tcggcgtcga
       61 ggtataccac catcggtcaa aacatgtgga ggagttcgtt gggcagtcgt ttacctatgt
      121 cattacggtc tgtgatcgcg ccaaggagtc gtgtcctgtc tttcctccgg cattcaaaat
      181 actgcattgg tcctttgagg accctgccac tataccactc gagaatagat cggaggcctt
      241 tcgacgagtg cgggatgaaa tcgccgaacg gattcgccaa tttcttaacg aagttgtgta
      301 aagcactcgt agatgcgtag ttagttggat ggaactggtg gcatccgcac aatgatctgt
      361 ggaggtggtg ccctcttagc aatttctttc tgagtttcaa tccgcatctt tgtcatttct
      421 agtgggcatc tgtttgtttt tggatggcct tcacttgcaa gttcccgctc tctcttgttg
      481 catccgcccc cattcttgcc gcgacaaagc acgccgaagg agacaacagc ccacaacgaa
      541 tcggctggca acatgccttt cgtcttttca gcagttcaat agcttacgtg ctgactgtag
      601 tcggcgtgcg acgtcggatc aagtcctgaa gggccgtgtg catctcggac tgtggcagat
      661 gaatgcgatc gccgtgtccg gccagtaccc attcaaacgg gtagtcgagg agtttccgaa
      721 tggactcaat caaggtccat tttctccaaa ggagacgagt gggagcttcc aacgatttgg
      781 tgtgtggatc ccaccagaga tgatctccgg tgaagagaaa tcgatcgcga tacagcaggg
      841 ccatgcatcc cgcggtatgg cccggcacgg gaatgatctg gaattctgat ccaactcgaa
      901 ccgtttcttc tcctccaatc acccgttctg cagtcggagc ggcatctcta tccgcctgat
      961 ggatgatgcg tttcgcaccg aaatgtgcgg catatttatc tgcatccgcc acgtcatcct
     1021 tgtgagtcag aaaaatatgg gcgattcctc cccgctgttc aaaggcttca acgagatgct
     1081 tgatgtaccg aggagagtcg atcagccagt tgccgtccgg atgttcaatg aaaaaactgt
     1141 tcgctccgaa cgatttctcc gaattgaagc cgcaataact cactccgttc tctatctgga
     1201 ggggaaagct cgccatggct gtttgcaatc gtgccttgtc gctgtgttca gtaccgatcg
     1261 atccgacggg acaggcaagc aatgcctggt aggcttgatg aatctgcggt tcactatccg
     1321 gctggcaacc gacagcggaa tagtcgccga cttcttcgaa gctcagcggc gccaactggc
     1381 gacacgtatc gcaattgatg caggtcgcat ccacatagaa gttaccggaa acattagaat
     1441 cgagtcgttt ctttcgatca gccatcagat tacttgtatg cctgcctttc tcggtcagcc
     1501 gcacgccaaa gataccaggc ggccaccgta cgatggggtt tccatcgttc gccatattgt
     1561 aacagctgtt ttggcgtggg catcaagggc cgccggtagg caatgcggaa gccattgcgc
     1621 aggccgaaat catcgaccgg caacacgtcc ggacgcccta actgaaaaat cagaaacatc
     1681 tcgaccgtcc atcgtccgat gccccgaact tcaatcagcc gatcaataat ggcatgatcg
     1741 tcgaggatgc cgacttcgcg tccggtcggg acggtcccat cgagggtctt gagcgcgaga
     1801 tcccgaagcg ccgacacctt cgcgcgtgaa aagccggctt tcctgatcga ccgcacgttc
     1861 acggcaagca attcatccgg ccgcgggaac cgacggccgg ggaagagcgc gatgaatcgt
     1921 ttgaggatgc tctcggcggc tttgtcgtgg agttgttgat aggcaatcgc gcgggcaagc
     1981 gattcgaacg gcgagcggcg gatcttgggc ttcagcatga acggaccgac ttcctcgatc
     2041 aaccggcgca tgaccggatc agcgtttgaa agatagtcgg tcgagtcgaa gctttcgtcg
     2101 tgcatcactc cagcgagatc cccattacct acatgacgat cccgccgccg gcctggatcg
     2161 tctgtcccgt gagccatcga gcttcctcac tcacaatgaa cgccacaaca tcggctatat
     2221 cttttggcac accgagccgt ttcatcggcg acagctgaat gccgatctgc cgatattcct
     2281 ccgtcatcat cccggtttcg gtaaaacccg gcgagacggt atttaccgta atccctttcg
     2341 gagccagttc ttgagccaat cccttagtgt attgctccaa cgctgccttg cttccgagat
     2401 aggctgtggc cccagcaaag tggagatgc
//
LOCUS       DASTWE010000137         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1587059, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000137 DASTWE010000000
VERSION     DASTWE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587059"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFT30_01540"
     CDS             <1..1081
                     /locus_tag="VFT30_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU5091336.1"
                     /translation="VSTGTFGLAFHLKRRKGFVPFANVELRTGLCQRVGSKEAVVERR
                     MTQDSTGPKSNASVIRIVEVGPRDGLQNESDVVPTAVKVSFVDALSKSGVAEIEAGSF
                     VSPRAIPQLADSDEVFRRIERVPGVVYSALVPNERGLERARAAAVNKIAVFTAASDSF
                     TRRNINCTIGESIERFKPVVFGAKRDGMTIRGYISTVTHCPFEGAVQPSQVLDVVRQL
                     LDLGVDEISLGDTVGKAAPRDIRRLLDEIVPRIDRSRLSLHFHDTCGMAIANVLTAWT
                     EYSIEAFDTAAGGLGGCPYAPGASGNVATEDVVYALKASGASLVVDERKVIAAASEIG
                     KALNLGLSSRLSQVQTEQTAYEGVA"
     gene            1078..1890
                     /gene="meaB"
                     /locus_tag="VFT30_01545"
     CDS             1078..1890
                     /gene="meaB"
                     /locus_tag="VFT30_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5091337.1"
                     /translation="MSPLSRAPFLCDIHDLMILAEQVKAGNIRAVSRLISLLENHPGG
                     REVLRLLNSASWGTRVIGVTGYPGAGKSTVVNRLVSFYRRQGLKVGVLAVDISSSVTG
                     GALLGDRIRMQEHALDHGVYIRSMATRGHHGGLAQATGDAALVLGAAGYEVILIETIG
                     VGQNEVDIVDVAHIVVAVVAPGLGDEVQAMKAGLLEVAHIIVVNKGDLPDADNTVRDL
                     REWCPNVLRTIATTGEGIPQLAAAMTAYQQPGGVGQSVKLEPTDVPNHGSLV"
     gene            1991..>2496
                     /locus_tag="VFT30_01550"
     CDS             1991..>2496
                     /locus_tag="VFT30_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5091338.1"
                     /translation="MPHQLLTISHHVGRITLHNPPANVLNLSVLKELDLVLSELEEDD
                     YVRVVIVTGIGRFFCAGADINELAHLNTEHGGAEFASCGQALLNRIERSDKPVLAAIN
                     GTCVGGGLELALACHVRVAVAGAILGLPEIKLGLIPGFGGTQRLPRIVGPSKAAEMIL
                     TGTSVSAE"
BASE COUNT          533 a          637 c          800 g          526 t
ORIGIN      
        1 tgtgtcgacg ggaacgttcg ggctcgcctt tcatctgaag cggaggaagg gattcgtgcc
       61 tttcgcgaac gtcgaactcc gaactgggtt atgccaacgg gtggggagta aagaggccgt
      121 tgtggagcgc cgcatgacgc aagacagcac cggaccaaaa tcaaatgcgt ctgtgatccg
      181 gatcgtcgag gtcggtcctc gagacgggct gcagaatgaa tcggacgtcg tccccacggc
      241 ggtcaaggtg tcgttcgtgg atgccttgtc caagagcggt gtggcagaga tcgaagcagg
      301 atcgtttgtg tcgcctcgcg ccattccgca attggcggat tccgatgagg tgttccggag
      361 gatcgagcga gtgcccggcg tcgtctattc agcgctagtg ccgaacgaac gagggttgga
      421 acgtgcgaga gcggcggcgg taaacaagat tgctgtcttt accgcggcct ccgattcgtt
      481 cacgcgacgg aacatcaact gcacgattgg cgagtcgatc gagcgattca agccggtggt
      541 gtttggggcg aaacgcgatg gcatgaccat ccgaggatat atttctaccg tgacccattg
      601 tccttttgag ggtgccgtcc agccctcgca agttctggat gtggtgcggc agttactcga
      661 tcttggagta gacgaaatct ctctcgggga cactgttgga aaggctgctc ctcgggatat
      721 ccgaaggcta ctagatgaaa tagtgccacg cattgatcga agtcgtctct cccttcactt
      781 tcatgacacc tgcggcatgg ctatagccaa tgtcctgaca gcatggacag agtacagcat
      841 cgaggcgttc gacacggctg ctgggggact cggagggtgt ccttatgcgc caggggcatc
      901 ggggaacgtt gcgacggaag acgtggtcta tgccttaaag gcatccggcg cctcgttggt
      961 cgtggatgaa cggaaggtca ttgcggcggc aagcgagatt gggaaggctc tgaacctcgg
     1021 actatcgtcg cgcctctcgc aggtacagac tgaacaaact gcgtatgaag gtgtggcatg
     1081 agtcccctca gtcgagcccc gttcctgtgt gacatccacg acttgatgat cttagccgag
     1141 caggttaaag ccggaaacat ccgcgccgta tcacgcctga tctccttgtt ggaaaaccat
     1201 ccaggcggca gagaagtgct gcgcctcctc aacagtgcct cgtgggggac gagggtaatc
     1261 ggagtaacag ggtaccccgg agccgggaag agcacagtgg tcaaccgctt agtgagcttc
     1321 tatagacggc aaggtttgaa ggtaggggtt ctggcggtcg atatcagtag ctctgtcaca
     1381 ggcggtgccc tgttgggtga ccgaatcaga atgcaggaac acgcgctgga tcacggggtg
     1441 tacatcagaa gcatggccac tcgtggacat catggagggc ttgcccaagc gaccggcgat
     1501 gcggcgttag tattgggagc agccggctac gaggtgatcc tgattgagac catcggagtc
     1561 ggccagaatg aagtcgacat cgtcgatgtc gctcacatcg tcgtggcagt ggtggcgccg
     1621 ggtctgggcg acgaggtcca agctatgaag gccggactat tggaagtcgc acacatcatc
     1681 gtcgtcaaca agggagatct tcctgacgcg gataatactg tacgagacct gcgggaatgg
     1741 tgtccgaatg tgttgcgtac catcgcgacg acaggcgaag ggattccgca gctcgctgct
     1801 gcgatgacgg cgtaccaaca gcccggcggt gttggccagt cggtgaagct cgaacctact
     1861 gacgttccga accatggaag cttggtatga taacaaaggg gaaagccgaa ggcgattatc
     1921 cgccggcgac aatgaagaag ggctgtgccc ctgagctggc ggaccacacg aagtgaggtt
     1981 tggaggactt atgccgcatc aattattgac gatttctcac catgtcggac ggatcacgct
     2041 ccataatccg ccggccaatg tgctgaatct ttccgtactc aaggaacttg atctcgtctt
     2101 gagcgaattg gaggaggacg actatgtccg ggtggtgatt gtgaccggta tagggcgttt
     2161 tttctgcgca ggcgccgaca tcaatgaact ggctcacctt aataccgagc acggaggagc
     2221 ggagtttgct tcttgcggac aggcccttct caaccgtatt gagcggtcag acaagccggt
     2281 tcttgccgca atcaacggaa cttgtgtcgg cggtggtctc gaactcgctc tggcctgcca
     2341 cgttcgggtg gcggtcgcag gcgccatatt ggggcttccg gaaatcaaac tcggtctcat
     2401 cccgggcttc ggaggaaccc aacggttgcc gcggatcgtt gggccttcca aggcggcgga
     2461 aatgatcctg acgggtacca gtgtctccgc agagga
//
LOCUS       DASTWE010000138         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_553059, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000138 DASTWE010000000
VERSION     DASTWE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553059"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFT30_01555"
     CDS             complement(<1..939)
                     /locus_tag="VFT30_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5091339.1"
                     /translation="MSAIAFPPSRVLFCFFATLFSSLMGGSRVEALSADSPGIRMLEE
                     IQTVITELAEQTKPSVVNLFPIPSTGRTREGPGERTPNASGSGSGLIVDSEGHIVTNN
                     HVIGDATEIEVRFSDKTKLIAHVIGKDADTDLAVLKVTTDRPLPSARFGDSADVKVGQ
                     WVLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPLFNLKGE
                     VIGINTAIINFAQGIGFAIPSNMAKQVIEQLLAKGKVVRGWLGVGIQPLTAELAKTFG
                     VAEGEGVLVNEVFEKDPAALAGIKPGDVIVRIDGAVV"
     gene            complement(982..1629)
                     /locus_tag="VFT30_01560"
     CDS             complement(982..1629)
                     /locus_tag="VFT30_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEU5091340.1"
                     /translation="MTFELLGRVHKELSITGHAFYETVLSISELVNRKVQIIRLHWQA
                     STLLQQIDDVTARLGQHIVVQVSDRMLYQNQPDSVVGVIDQMLADALSRVQELKQSLI
                     GVDAQIRELKVETVHQDLLSLQRDLALRAGGIERLTVVRGAAAAGQPPSALPRYSSAY
                     VATVLRGSFLLAPTDDLLFRPGDIVVLIGSQAELNQLVSWFTGQRPLTTSLARSG"
BASE COUNT          419 a          485 c          481 g          409 t
ORIGIN      
        1 aacgacggca ccgtcgatcc ggacgatgac atcgcccggc ttgatcccag ccaacgctgc
       61 cgggtctttc tcgaagactt cgttcaccag gactccttcg ccttcggcca ccccgaatgt
      121 tttcgccaac tcagccgtca acggctgaat tccgacgccg agccagcccc gcacgacctt
      181 gcccttggca agtaactgtt cgatgacctg cttggccatg ttcgagggaa tggcgaatcc
      241 gatgccttgg gcgaaattga tgatcgccgt gttgatgccg atcacctcac ctttgaggtt
      301 aaagagcggg cctcctgaat taccaggatt gatagaggca tcagtctgaa tgaagttttc
      361 gtaccgtgag agattgatgt tctcgcgacc aatgccgctg accaccccga gcgtcacggt
      421 ccggtccagt ccgaacggat tgccgacggc caacacccat tggccgactt tgacgtcggc
      481 cgaatcgccg aatcgcgcgc tgggtagcgg tcgatcagtg gtcactttga gcacagctaa
      541 atcggtgtcc gcatccttgc cgatcacgtg agcgataagc ttcgtcttgt cggaaaaccg
      601 cacctcaatt tcagtcgcat cgccgattac atggttgttc gtcacgatat ggccttcact
      661 gtcgacaatc aggcccgagc cggagcccga agcattcggc gtccgctcgc cgggtccttc
      721 acgagtccta ccggtgctag ggatgggaaa caggttgacg acagaaggct tggtctgctc
      781 agcgagttcg gtaatgacgg tttggatctc ttccagcatg cgaatgccgg gtgagtcggc
      841 cgataatgcc tcgacccggc tgcctcccat gagagaggaa aagagagtag caaaaaaaca
      901 gaacaaaaca cgtgatggtg gaaatgcgat agcagacatc gaccgttatt gtatctgatc
      961 tctgtcgatg tgcctactgt cttatcccga tctagcgagc gacgttgtca gagggcgctg
     1021 gccggtaaac catgagacaa gttgattgag ttctgcttga gagccgatca aaaccacgat
     1081 gtcgccgggt cggaacagga gatcatccgt gggagccaac agaaatgatc ctcgtaatac
     1141 cgtggccacg tatgcagagg aataccgggg aagggcgctg ggcggctgtc cggccgcggc
     1201 agcgcctcga acgacggtca gccgttctat acctccagca cgaagcgcca aatcacgttg
     1261 gagagagagt aggtcttggt ggaccgtctc gactttgagt tcacgaatct gggcatcaac
     1321 tccgatcaac gattgcttca actcttgaac gcgagaaagg gcatcagcaa gcatctgatc
     1381 gatcacaccg acgacagaat cgggttgatt ttggtacaac attcgatcag aaacctgcac
     1441 gacgatgtgt tggccgagcc tggccgtgac atcgtcaatt tgctggagca aagtagaagc
     1501 ctgccaatgc agacgaataa tctgaacctt tcgattgacc agttcggaaa tagagagaac
     1561 agtttcatag aacgcatgtc cggtaattga aagctcttta tgtacacggc caagaagttc
     1621 gaacgtcata gtctgataat atatattatg ttaactatct gttttcttac tcgcccgctc
     1681 gtgctttccg tgtggcttct ttcgcccggc attccttata cctacatagc cgatcgtagc
     1741 tcgaaggtca atcgtgcagc ggttttcctt gagcttcggc aaatcttttg acac
//
LOCUS       DASTWE010000139         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1231622, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000139 DASTWE010000000
VERSION     DASTWE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231622"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFT30_01565"
     CDS             <1..568
                     /locus_tag="VFT30_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5091341.1"
                     /translation="SIMGSDEALSWPLETTMLDYELELACVIGRKGRDISERLAEDYI
                     AGYTIMNDFSARDIQFQEMACRLGPAKGKDFATALGPCLVTPDEIADLGALTMIARVN
                     GEVWSRGRFGTIHWSFAQMISHVSRDETIYPGDVFGSGTVGGGCGLELDRYLKPGDVV
                     ELEIQPIGVLRTRVMSATPGGENAGQHD"
     gene            528..>1151
                     /locus_tag="VFT30_01570"
     CDS             528..>1151
                     /locus_tag="VFT30_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091342.1"
                     /translation="MQHQGVKMLVSTIEQALAEVVDRHRVLYEDRTIDARLKAIIRAG
                     GHTNVTEDDVRHILRKSRSVFFDTHVEVVSGHHTATYLRFESIARFPELIRQLTDDMA
                     DWIRKTFQYHPITGLVATSSAAELLAQGIVDVLHDTMGLRVALTPYSCETGRIGTEIT
                     VGGIKAGERFLALNDVTTRGNCVSKLGKVITDHGGLLAGMMVFARRDS"
BASE COUNT          249 a          272 c          374 g          256 t
ORIGIN      
        1 ctcgatcatg ggatcggacg aggctctgtc ctggccgctg gagacgacga tgttggacta
       61 cgagttggaa ctggcatgtg tcatcgggcg gaagggcagg gacatctccg aacggctggc
      121 ggaggactac attgccggtt acacgatcat gaacgacttc agcgcgcgcg atattcagtt
      181 ccaagaaatg gcctgtcggc tgggtccggc gaaaggcaag gatttcgcga cggcgctggg
      241 cccctgtctc gtaacccctg atgagatcgc ggacctcggt gccttgacga tgattgcaag
      301 ggtcaacggt gaagtatggt cgcgaggacg attcgggacg attcactggt cttttgctca
      361 gatgatctct cacgtgtcgc gggacgaaac aatctatccg ggagatgttt tcggttcggg
      421 aacagtggga ggtggatgcg ggttggaact ggaccgttac ttgaaaccgg gcgatgtggt
      481 ggaattggaa atccagccga tcggggttct taggacgcgg gttatgagtg caacaccagg
      541 gggtgaaaat gctggtcagc acgattgagc aggccctcgc agaagtggtg gatcggcatc
      601 gcgtgctgta tgaagaccgg acgatcgatg caagactgaa agccatcatc cgcgctggtg
      661 gccacaccaa tgtcaccgag gatgatgtgc gtcatattct gcgtaagtct cgatctgtct
      721 ttttcgatac tcacgtggag gttgtgtcag gtcaccacac ggcgacgtat ctgcgtttcg
      781 aatccatcgc tcgttttcct gaattaatcc gtcagttgac cgacgatatg gcggactgga
      841 tcaggaagac gtttcaatac catccgatca cgggtctcgt ggcgaccagt tcagcagcgg
      901 agttgctcgc acaaggaatt gtggatgtcc ttcacgacac gatggggttg cgtgtggcac
      961 tgacgccgta cagttgcgag acaggacgga ttggaacgga aatcaccgtc ggcgggatta
     1021 aagccggcga gcgattttta gcgctcaacg atgtcacgac gcggggcaat tgtgtcagca
     1081 aactcgggaa agtcatcacg gaccatggag gtttgttggc ggggatgatg gtctttgccc
     1141 gccgtgacag c
//
LOCUS       DASTWE010000140         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_843835, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000140 DASTWE010000000
VERSION     DASTWE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843835"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFT30_01575"
     CDS             complement(<1..1298)
                     /locus_tag="VFT30_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU5091343.1"
                     /translation="MNGIITEVLSVSDGQPLSLVSLEPVLISGMRSGSRQVREWIPLD
                     RVPPSLIKTLLIIEDRRFFSHFGVDPIAIGRALWINMTRGGVVQGGSTLTQQLAKNLY
                     YSPKRTIGRKLRELMAAMALEFKYRKEEILESYVNEIYLGQAGPVSIYGVGEAAHRYF
                     SKNVDELSIDEMALIVGLIKGPNIYSPVKDIELATKRRNVVLRRLREAGILSEDVVAQ
                     AMNWPVKVMLNQDVLTDAPYFVDHLLREIEQGIGMGIPDGARIYSTLDPRVQRIVAQV
                     LQEGLTKLEKRYPTLAGVEPPLQGAAVVLDVKTGHVLAMVGGRNYRLSQFNRAVQAHR
                     SAGSLFKPFVYLAGFEVARDQGTTGLTPATLLVDEPVTLESGIGSWSPQNYDRQYRGQ
                     VTVRTALEQSLNVPAVRTAHRTGITALTNLLHAFGITTPLA"
BASE COUNT          318 a          402 c          360 g          327 t
ORIGIN      
        1 gccaacggtg tggtgattcc gaacgcgtgt aacagatttg tgagcgcagt aattccagtt
       61 cggtgtgcgg tccgaacggc aggaacgttc aaggactgct cgagtgccgt tcggaccgtt
      121 acttgccctc tgtactgccg atcgtaattc tgaggcgacc aggatcctat gccggattcc
      181 agtgtcactg gctcatccac tagtaatgtc gctggagtca gcccggtcgt accctgatca
      241 cgtgctactt caaatccagc aaggtacaca aaaggtttga agagagaccc cgccgatcga
      301 tgcgcttgca ccgcacggtt gaactggctt aggcgatagt tacgaccgcc gaccatcgcc
      361 agcacgtggc cggttttcac atccagtacg accgcagctc cctgaagtgg cggctcgact
      421 ccggccagag tcggatagcg cttttcaagt ttcgtcaatc cttcctgtag cacctgagcg
      481 acgattcgct gaaccctggg atcaagggtg gaataaattc gtgctccgtc cggtattccc
      541 attcctattc cctgctcgat ttctctgagc aggtgatcga caaagtatgg cgcatcggtg
      601 aggacatctt gattcaacat gaccttcacc ggccagttca tggcctgagc cacgacgtct
      661 tccgacaaga tacccgcttc cctcagacgg cgaagcacca cgtttcgacg cttcgttgcc
      721 aactcaatgt cttttacagg ggaatagata ttgggcccct tgatcaatcc gacgatcagc
      781 gccatttcat cgatcgacag ttcgtcaaca ttcttactga aatagcgatg agcggcttcc
      841 cccacaccgt agatcgaaac cggtccggct tggccgagat agatctcatt cacataactc
      901 tccaggattt cttcttttcg gtatttgaac tcgagagcca tcgcggccat caattccctg
      961 agtttccgtc cgatggttcg cttcggagaa tagtaaaggt tcttggccaa ttgttgggtg
     1021 agcgtgctgc ctccctgcac gactccgcca cgcgtcatgt tgatccaaag tgctcggcca
     1081 atcgcgatgg ggtcaacccc gaaatgggaa aaaaaacgac gatcctcgat gatcagcagc
     1141 gtcttaatca gagagggagg gactcggtcc aggggaatcc attcccggac ctgcctggac
     1201 ccggaacgca tcccgctgat caaaaccggc tcgagagata ccaaggaaag cggttggcca
     1261 tcggacactg acaacacctc ggtgatgatg ccgttcacca gtatcaaacg gactgatcgt
     1321 gcaggaagcc ggttttcctc ctgagcatgc agaaagatct caattgagtc ttttgttgcg
     1381 aaatactcac ctgccacccg tggtgcc
//
LOCUS       DASTWE010000141         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1166874, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000141 DASTWE010000000
VERSION     DASTWE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166874"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFT30_01580"
     CDS             <1..1180
                     /locus_tag="VFT30_01580"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEU5091344.1"
                     /translation="RNTAPAIALAALEVLTRDPDGLMLVVPADHVVTGQRDFETAVRF
                     ASQLAAEGYLVTFGIKPIRPETGYGYIKPKDNALLGKQGKLRGYLVQKFVEKPNAVKA
                     AQYVKTGGYFWNSGMFVWRAATILAEIGLHQPAIMAAMERIKKLRIDSAPKQAIDDVY
                     RQIVSVSIDTGVMERSSKAAVVPVTFQWSDVGSWGSLDEVAEKDKAGNVVTGRVIDLE
                     SKHSIVYADRRVVATIGLQNMVVVDTPDATLVCPKSRAQDVKKVVDILKRQQAPEHLE
                     HLTVQRPWGSYTVLEEGTGFKVKRVTVNPGGRLSLQMHHQRSEHWVVIAGTARVTRDQ
                     EVFDLHVGESTAIPVKTKHRLENSGQETVHIIEVQNGPYLGEDDIVRFKDDYGRIASG
                     "
     gene            1247..>1557
                     /locus_tag="VFT30_01585"
     CDS             1247..>1557
                     /locus_tag="VFT30_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEU5091345.1"
                     /translation="MGLFREYDLRGIVGSELTEDLAERLGRAYSTYVNERGVKTISVG
                     RDGRLSSPTLHKALLKGLLAGGLDVIDIGICPSPLVYFSLFTLPIGGGIMITGSHNAA
                     E"
BASE COUNT          353 a          362 c          497 g          345 t
ORIGIN      
        1 acgtaacacg gcccctgcca ttgcattagc ggcgctcgag gtcctgacac gtgatcctga
       61 cggcctcatg ttggtggtcc ctgccgacca tgtggtgacg ggacagcgcg actttgagac
      121 tgcggtccga ttcgcttcgc aattggctgc ggagggctac ttggttacgt ttgggatcaa
      181 gccgatcaga ccggagacgg gttatggcta tatcaaaccg aaagacaacg ctctgcttgg
      241 taaacagggc aagttgcgag ggtatctcgt tcaaaaattc gtggagaagc ccaacgccgt
      301 caaggcggct cagtacgtga aaacgggtgg ctatttttgg aacagcggca tgtttgtctg
      361 gcgcgccgcg acgatcttgg cggaaatcgg ccttcaccag ccggcgatta tggccgcaat
      421 ggaacggatc aagaagctta gaatcgacag tgccccaaag caggcgatcg acgatgtcta
      481 tcgccaaatc gtgtcggtct ctatcgatac cggtgtgatg gaacggtcgt cgaaggcggc
      541 tgtagtgccg gtgacgtttc agtggtctga tgtcgggagt tggggcagct tggacgaagt
      601 ggccgagaag gataaggccg gcaatgtagt caccggacgg gtgatcgacc ttgagagcaa
      661 gcactccatt gtctatgcag atcggcgcgt cgtcgcgacg atcgggctac aaaatatggt
      721 agtggtggac actccggatg caacattggt gtgtccgaaa tctcgagcac aggatgtgaa
      781 aaaggtcgtc gacattctga aacggcagca agcgcctgag catttggaac acctgacggt
      841 tcaacggcct tgggggtcat acacagtgtt ggaagagggg acgggtttca aggtgaaacg
      901 tgtcacggtg aaccccggtg ggcggctctc tcttcaaatg catcatcagc gtagcgaaca
      961 ttgggtggtg attgccggta ctgcgcgcgt cacaagagat caagaagtct tcgatttgca
     1021 cgtcggcgaa agtacggcca tcccggtgaa aaccaagcat cgattggaaa attccgggca
     1081 ggaaacggtg catattatcg aggtacagaa cggaccctat ctcggtgaag atgatattgt
     1141 gcggttcaag gacgattacg gtcggatagc gagcggttga tccagggtga agttggggtc
     1201 aggccggatg cttgtgagtt gagaactgct cgttaaagga gggcttgtgg ggttgtttcg
     1261 agaatatgat ctccgtggaa tcgtcgggag tgaattgacc gaggatctgg cagaacggtt
     1321 gggccgagcc tattccacgt atgtgaatga gcgtggagtg aaaacgatca gtgtggggcg
     1381 cgatggtcgg ttgagttccc cgacccttca caaggcgctt ctgaaggggc tactcgccgg
     1441 cggtctcgat gtcatcgaca tcggaatttg tccctccccc ttggtgtact tttcattgtt
     1501 taccctgccc atcggcggcg gcatcatgat taccggaagt cacaacgcgg cagaata
//
LOCUS       DASTWE010000142         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1102474, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000142 DASTWE010000000
VERSION     DASTWE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102474"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFT30_01590"
     CDS             <1..630
                     /locus_tag="VFT30_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU5091346.1"
                     /translation="VTHVMQECNIGLFPVELHAEQSRVVRVVMSQPKPEFLDPIDAID
                     DVYLVGGALGLPKHVIADMKWPLQVVSTGLPVLIVPVRTLTAVRSIHPDASAIITVCE
                     RFGANGIMVFTTVTVESFASVHARMFAPKIGILEDPATGSAGGALGAYLVQNGVVEVG
                     PRTDILIEQGYEIDRPSRILVQVESDDDVIQGVKVGGQCVMVVEGVLKF"
     gene            848..1282
                     /locus_tag="VFT30_01595"
     CDS             848..1282
                     /locus_tag="VFT30_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5091347.1"
                     /translation="MATVSQIANKRPKSIGPKISITSAAKTMRQARVGSLLVKKGKQL
                     IGIVTDTDIVRRAVATGKPLGKLTVEKIMTTPICTIEGNQSVDDAQDMMAELGVRHLG
                     VTRNGEITGVVSVRDLLQHYKRYAQSKISTEVTYDEPKITQD"
     gene            complement(1339..1851)
                     /locus_tag="VFT30_01600"
     CDS             complement(1339..1851)
                     /locus_tag="VFT30_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU5091348.1"
                     /translation="MTIPSHHEHKDHAPNSIGCMVITCSDTRTPETDTSGQLIRKLLG
                     ERGHTVVGYHLVKDEPAQIQLWIARGTVNAEVQAIIINGGTGISRRDSTYEAVDEMLE
                     KRLDGFGEIFRLLTYQEIGSSAIMSRATAGVINGRVLFSTPGSENAVRLAMEKLILPE
                     LGHLVKELTK"
     gene            complement(1942..2268)
                     /locus_tag="VFT30_01605"
     CDS             complement(1942..2268)
                     /locus_tag="VFT30_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEU5091349.1"
                     /translation="MPKPKYHILVCTNSRPPGHPKPSCGSAGAAQLLMAFNMGLMQRS
                     VPPGEVLVSATGCLGPCEQGPTVVVYPDNTWYSKVTETDVATILDEHVAKGTPVAKLN
                     PDAVWK"
     gene            complement(2308..2751)
                     /locus_tag="VFT30_01610"
     CDS             complement(2308..2751)
                     /locus_tag="VFT30_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEU5091350.1"
                     /translation="MKFFLYGDLLNPSQLKRRAPEHRFLYLATLSDHTVKFCRWSSQW
                     RCGLASIVPSQGEKTWGGVFELTDEDIKIMDQFEQDVPQGAYRHLQVTVSSANGEKEL
                     VTTYAANPIGKFKPKDHYLDWVLKGLKQWKFPEEVIQEWESYRPR"
BASE COUNT          649 a          763 c          800 g          681 t
ORIGIN      
        1 gtcacgcatg tcatgcagga atgtaatatc ggtctattcc cagttgagtt gcatgcggaa
       61 cagagtcgtg tggtgcgggt cgtcatgtct caacccaaac ctgaatttct cgatcctatc
      121 gacgctatcg acgatgtgta cttggtcggt ggagctctcg gcttgccgaa acacgtgatc
      181 gccgatatga aatggccgct tcaagtcgtg tcgaccggct taccggtctt gatcgtgccg
      241 gtacgaacgt tgacggccgt ccggtcgatc caccctgatg cttcggccat catcaccgtc
      301 tgcgaacgat tcggggccaa cgggattatg gtcttcacga cggtgacggt tgaatcgttc
      361 gcttccgtcc acgcccggat gttcgctccc aaaattggaa tcttggagga tcctgcgacc
      421 gggagcgccg gcggtgcact cggtgcatac ctggttcaga acggcgtcgt tgaagtcggg
      481 ccgaggacgg acatccttat tgaacagggc tacgaaatcg atcggccttc caggattctg
      541 gtgcaggttg agtcggacga cgacgtgatt caaggtgtga aggtcggtgg acagtgtgtg
      601 atggtggtgg aaggagtatt gaagttttga aatagggaca attgatcaac gacgaagaaa
      661 aagagatcca tgagcttcct gtgcttgcgc gccgcgcatg cagaccatcc tgcttagccg
      721 cattactatc tttgaagtga acggcctcgc acgctccgct cattcatgat aatctacgcc
      781 cgacgaatgt ggctgatatg tgaaccagca gtcagccgcg atcatatgaa aacgaaaggg
      841 ggaggggatg gctacagtta gtcagattgc aaacaagcgt ccaaagtcca tcgggccgaa
      901 gatctcaatc accagtgccg ccaagacaat gcgccaggca cgagtgggat cgctgttggt
      961 gaagaagggc aagcaattga ttggtatcgt caccgatacc gatatcgtgc gccgagctgt
     1021 ggcgacaggg aagccgctcg ggaagcttac tgtcgagaaa attatgacga caccgatttg
     1081 cacgattgag ggaaaccagt cggtcgatga cgcccaagac atgatggctg aattgggtgt
     1141 ccgtcatctc ggcgtcacca ggaacggcga gatcaccggc gtcgtctccg tgcgcgatct
     1201 gctgcagcac tataagcgtt acgcgcaatc gaagatttca actgaggtga catatgacga
     1261 accgaagatc acgcaggact gacgcagtac cgagcgtgag ctttgagtgc tgagcttaca
     1321 tagtaagcac ccaacacatc actttgtcag ttctttgacg aggtggccca gctcgggaag
     1381 gatgagcttt tccatcgcga ggcggacggc attttctgag ccgggagtgg aaaagagtac
     1441 gcggccattg atcacaccgg cagtcgcccg actcataatc gccgatgaac caatttcttg
     1501 ataagtgagc aaccgaaaaa tctcgccgaa tccatcgagc cgcttttcca acatttcatc
     1561 caccgcttcg tacgtggagt cccgccggga aataccagtt ccaccattga tgatgatggc
     1621 ctgcacttcc gcattgaccg tacctcgagc gatccagagc tggatctgcg ccggttcgtc
     1681 cttcacgaga tgatagccta cgaccgtatg gccgcgctct cctagaagct tgcgaatcaa
     1741 ctgaccgctg gtgtcggttt cgggcgtacg ggtatcactg caggtaatga ccatgcatcc
     1801 gattgagttg ggtgcatggt ctttgtgttc gtgatgactt ggtatggtca tgtggtaaga
     1861 aatacgctat cagcaatcaa tttaaacaat caagctttca atctcgtccg accgctcaaa
     1921 gcgggccgtc attggctaag tttatttcca cactgcgtct ggattcagtt tcgctactgg
     1981 ggttcctttt gcgacatgtt catcaaggat ggtggcgacg tccgtctcag ttaccttgga
     2041 ataccaggta ttgtcgggat agacgacgac cgtcggccct tgctcgcagg gaccgaggca
     2101 gcctgtcgcg ctcaccaaca cctcccccgg tgggacggat cgctgcatca gccccatatt
     2161 gaaagccatc agcagctgcg ccgcaccggc cgacccgcaa gagggtttcg gatggcctgg
     2221 gggacgggaa ttggtacaga cgagaatatg atacttcggt tttggcatag tctcctcaat
     2281 aaaaatggaa catgtggcac ggtccgctta ccgtggccga tacgactccc actcctggat
     2341 cacttcttcc ggaaacttcc actgtttgag ccctttcagc acccagtcca ggtagtggtc
     2401 tttgggttta aacttcccga tcgggtttgc tgcgtacgtg gtgacgagtt ccttttctcc
     2461 gttcgcgctc gaaacggtga cttgcagatg gcgataagct ccttgtggca catcctgctc
     2521 aaactgatcc atgatcttta tgtcttcatc cgtcagttca aatactccac cccaggtctt
     2581 ctcaccttgt gaaggtacga tactggcgag cccgcaccgc cactgtgacg accatcggca
     2641 aaatttgacg gtatggtccg acaaggtcgc gaggtagaga aatctgtgtt ccggggctcg
     2701 ccgtttgagt tgtgagggat tgaggagatc accgtacaaa aagaacttca tgcaccgtac
     2761 tcattctgtg cccgaccgtc ctgtagtctg ctcgtacttg taacacaccg gcttttcatc
     2821 agacaagcgg ctcgctgcgc ccaattctcg aagcgacatt gacatggcgt ctgggccttc
     2881 ctaagatgcc gtg
//
LOCUS       DASTWE010000143         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_520822, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000143 DASTWE010000000
VERSION     DASTWE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520822"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VFT30_01615"
     CDS             <1..1606
                     /locus_tag="VFT30_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HEU5091351.1"
                     /translation="RQWKQIKLAHLARMALDWAAIAPRPGEAPKAHPYAADLDLFGAH
                     SLTHLLDTTVSDHGRGRLHSWLLEQPPTPSHWHTRQSLVKELASRSLFRDRLALEAKL
                     TGDQEINGRRLAAVLEHPIGLPHINTILIVQSLLALATISLALATMFGWLPGYWMFSF
                     AAYVLIYFMTDQGEELLEHAVGLHHETERLATVLGYIGQHTRRRDTALAYTWTNLIGE
                     ASPVLHLQQAARTLHAISIKAHPLVHLAVNALCPWDLWFTRRLIHIQHKIKTALPQWL
                     DCLAEVEAASALATFAYLHPNYAWPGPLIAPGEQNGTPPAFDADQLGHPLLTAKTRVT
                     NDVHLKGLGSIHLITGSNMSGKSTFLRTIGINLCLAQAGAPVCAHSFEWTWSRLACCI
                     RVDDSLDAGLSFFYAEVKRLKTILDATKDITAPPVLFLIDEIFKGTNNRERLIGSRAY
                     ITALSQGNGFGLVSTHDLELADLEPVVPRLINAHFQETVSAGTLEFDYKLRPGPCPTT
                     NALRIMELEGLPISPAPNIRQNGPHG"
     gene            1603..>2617
                     /locus_tag="VFT30_01620"
     CDS             1603..>2617
                     /locus_tag="VFT30_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5091352.1"
                     /translation="MTTSAGSETQPFSHPLRGLLIAQFCGAFNDNAWKLMVALLAIRQ
                     ATAGMAQGPELETVAQTQTAMAFVIFTLPLMLLSLVGGTLADRVSKRTVIISIKVVEV
                     FLMAAGTVALWLNPAGGVLPLIVLCGMGVHSALFSPSKYGILPELIPHERLATGNGLL
                     EMWTFAAILTGTAAGGFLLQIAGDQTWLAPLALTGLSVIGLTTAFSIPHVSPARTTGG
                     VGVTVQGAWTAIQSERMLRMAIPMEIMFWTIASLFGQNILVYAKAVLHLSDGMSGLPL
                     TILSVGIGIGAMLVGRISQNRVEYGLIPLGAIGVFLILLSLGILAPPLTGTFLMMGVL
                     GISS"
BASE COUNT          546 a          797 c          684 g          590 t
ORIGIN      
        1 gcggcaatgg aaacagatca aactggctca tctggctcgg atggcgctgg attgggccgc
       61 gatcgctccg agacccggtg aagctccgaa ggctcatcca tacgctgctg acctggatct
      121 cttcggtgca cattcgctga cacatctcct cgacaccacg gtatccgatc atggcagagg
      181 gcgcctgcat agttggctgt tggagcagcc tcctaccccg tcacattggc acacacgcca
      241 gtctttggtg aaagaactgg catcccgctc cttgtttcgt gaccgtctcg cgctcgaggc
      301 caaactgacg ggagaccaag aaatcaacgg caggcggttg gctgctgtgc tggaacatcc
      361 gatcggcttg cctcacataa acaccatcct cattgtccaa agtctccttg cccttgcaac
      421 aataagtctt gccttagcta cgatgttcgg ctggctcccc ggttattgga tgttttcgtt
      481 cgcggcctat gtgctgattt acttcatgac tgatcaaggg gaagagttgt tggagcatgc
      541 cgtcgggctt caccatgaaa ctgaacggct cgccacggtc ctcggctaca ttgggcagca
      601 tacaagacga cgagacacgg cgctcgctta cacctggaca aacctgatcg gtgaagccag
      661 tcccgtgctg catctacaac aagccgctcg gacgcttcat gctatcagca tcaaagccca
      721 tcccctcgtc catttggccg tcaacgcgtt atgcccatgg gatctgtggt tcactcgacg
      781 actgattcac atccagcaca agatcaagac tgccctccct cagtggttgg actgcctggc
      841 agaagtcgaa gcggcatcgg ctttggctac ctttgcctat ctccatccca attacgcatg
      901 gcccggtccc ctcatcgcac ccggcgaaca gaacggcaca cctcctgctt tcgacgccga
      961 ccaactcggc catccgttac tgacggcaaa gacccgtgtt actaacgacg ttcatttgaa
     1021 gggactcggc tcgattcacc tgattaccgg ctcgaacatg tccggcaaga gtacattcct
     1081 gcggacgatc ggtatcaatc tctgcctggc gcaagccgga gcccctgtct gcgcccattc
     1141 atttgagtgg acgtggagcc ggctggcctg ctgcattcgt gtcgatgatt cgctggatgc
     1201 cggtctgtcg ttcttctatg ccgaggtcaa acgactcaag acgatcctcg acgccacaaa
     1261 agacataacc gcgccacccg tgctgttttt gatcgacgag atcttcaaag gcaccaacaa
     1321 ccgggagcgg ctcatcggca gccgtgccta tataaccgca ctgtcgcaag gaaacggttt
     1381 cggcctcgtg agcacccatg atctggagtt ggcggatttg gagcccgtcg taccaagact
     1441 catcaatgcg cacttccagg aaactgtatc ggccggcaca ctcgagtttg actacaagct
     1501 cagaccaggc ccgtgcccca cgacgaacgc gttgagaatt atggaattgg aaggcctgcc
     1561 tatttcccca gcaccaaata tcaggcagaa tggtccccac ggatgaccac ctcggccggt
     1621 tctgaaaccc aacccttttc acatcccctc cggggtcttc tgatcgccca attctgcggc
     1681 gccttcaacg ataacgcgtg gaaactgatg gttgccttgc tcgccattcg gcaagccacg
     1741 gcaggtatgg cacagggacc agaattggaa acggtcgctc agacgcagac cgcgatggca
     1801 tttgtcattt tcacactgcc gttaatgctc ctctctctcg tcggaggcac attggccgat
     1861 cgcgtgagca agcggacggt catcatctcg atcaaagtcg tcgaggtttt cctcatggcc
     1921 gccggcacag tcgcactctg gttgaatcca gccggaggtg tcctgccctt gatcgttcta
     1981 tgcggcatgg gtgtgcacag cgccctgttt agtccttcga agtacggaat ccttcccgaa
     2041 ttgatcccgc atgagcgcct agccaccggc aacggcctct tggagatgtg gacctttgcc
     2101 gcaattctga cgggaaccgc agcaggggga ttcctgttac aaatcgccgg ggatcaaacc
     2161 tggctggccc cactggcatt gaccggtctc tcggtcatcg gcctcacgac cgcctttagc
     2221 attccacacg tgtcacccgc ccgcacgacc ggaggcgtgg gagtcaccgt ccaaggcgca
     2281 tggacagcaa ttcaatccga acggatgttg cgcatggcca ttcccatgga aatcatgttc
     2341 tggacgattg cgagcctgtt cggacagaac atactcgtct atgcaaaagc cgtcttgcat
     2401 ttgtcagatg ggatgtcggg tcttcccctt accatcctgt cggtgggcat cggaatcggg
     2461 gcaatgctcg ttggacgaat ctctcagaac cgggtcgagt acggactgat tccgttaggt
     2521 gccatcggcg tgtttctcat attactgtcg ctaggaatcc tggcaccccc tttgacagga
     2581 accttcctca tgatgggagt gcttggaatc tccagtt
//
LOCUS       DASTWE010000144         1301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_682382, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000144 DASTWE010000000
VERSION     DASTWE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682382"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1301)
                     /locus_tag="VFT30_01625"
     CDS             complement(<1..>1301)
                     /locus_tag="VFT30_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091353.1"
                     /translation="SETDGILSVGNGNRAIANPFIRVVDDTLKCNKLKVGGVEFALDG
                     SIESGGVLTLEKDKLARFKNDGAVSFDFDGHLKCNKLIVDKLEGVVISNLPVSLPEDP
                     VLDALLVYEDGATSWEPNTVRVKHSNVAPGTAHFYDGTDLRPTPLTFDGRGRENGPGV
                     LLHGETGTVLVRTAGDDAILTSDGGWKTEPTLLKLGPGSAGDVVTLDDSIPIFKRPDW
                     MSTKDPGKKVSGSHFVVFGTDNLEPSYIVDDGGADSRYLGVSRNSSIDIVSDIGFLAP
                     SMTRRYSLLNGKLEAKMSVPIPDQSEEDQHLARFDDGKLVAEWVPTNFIVPPPSGIVD
                     ASNASIVNVAEPVDPMDAVTLNYAKQHCCWDGDAGNARVCNVGNPASETDGVPFRYAK
                     ELYSRGLNILSTDNFGAGGDKITNIADPVNLADAANVAYLSS"
BASE COUNT          289 a          360 c          335 g          317 t
ORIGIN      
        1 gaacttaaat acgcgacgtt ggccgcatcg gctaggttaa cgggatcggc aatgttggtt
       61 atcttgtcgc caccggctcc gaaattgtcg gtgctgagaa tgttgaggcc acgagagtag
      121 agttccttgg cgtatctgaa aggtacgccg tcagtttcgc tcgcgggatt gccgacgtta
      181 catactctcg cgttaccggc atctccgtcc cagcagcaat gttgtttggc gtaatttaac
      241 gtgacggcgt ccatcgggtc gacgggttcg gccacattaa cgatactggc attcgacgca
      301 tcaacgattc ccgacggtgg tgggactata aaattagtcg gcacccactc ggccaccaat
      361 ttaccatcgt caaaacgagc caagtgttgg tcttcttcgg actggtcggg tatggggacg
      421 ctcatcttgg cttccagctt gccgtttaag agagagtatc gacgagtcat cgagggtgct
      481 agaaaaccaa tgtctgacac gatgtcgatg gacgaatttc tggatacgcc cagatacctg
      541 ctgtctgcac cgccgtcgtc cacgatgtag gaaggttcga ggttgtccgt accaaagacc
      601 acaaagtgtg atccggatac cttctttccg ggatctttcg tcgacatcca gtccggtcgt
      661 ttgaatatcg ggatcgaatc gtccagggta acgacgtcac ccgcgcttcc cgggcccaat
      721 ttcagcaacg tcggctccgt cttccaacct ccgtccgacg tcagtatggc atcatcgccg
      781 gcagtcctca ccaaaaccgt accggtttcc ccgtgcaata acacaccggg tccattctcg
      841 cgtccgcgcc cgtcaaatgt cagtggagtc ggtctgagat cggtgccgtc gtaaaagtgg
      901 gctgtaccag gcgcgacgtt ggaatgttta acgcgaacgg tgttgggttc ccacgaggtg
      961 gcgccatctt cgtacacgag aagagcgtcg agtacgggat cttctggcaa cgataccggc
     1021 aagttcgaaa taaccactcc ttccaatttg tccacgatca acttattgca cttcagatga
     1081 ccgtcgaaat cgaacgatac tgcgccgtcg tttttaaaac gagctaactt gtccttttcg
     1141 agtgttaaaa cgccgccaga ttctatactg ccgtccaatg caaactccac gccaccgacc
     1201 tttaacttgt tacactttag cgtatcgtcg accacgcgaa taaaggggtt ggcaatcgcc
     1261 ctgttaccgt taccgacact cagtataccg tcggtttcgg a
//
LOCUS       DASTWE010000145         1290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1587289, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000145 DASTWE010000000
VERSION     DASTWE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587289"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>1290
                     /locus_tag="VFT30_01630"
     CDS             66..>1290
                     /locus_tag="VFT30_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEU5091354.1"
                     /translation="MNTSEPSTSSNFIRDLVAADRAVGKHGGRVVTRFPPEPNGYLHI
                     GHAKSIVLNFGIANETPGGVCHLRFDDTNPTTEDPEYVQAIQEDVRWLGFDWHGKMFY
                     ASDYFEQLYAFAIVLIKKGKAYVDSLTADQIREHRGTLTEPGRDSPYRTRSPEENLDL
                     FARMRAGEFADGTHVLRAKIDMGSPNINLRDPILYRIRHATHYRTGTAWCIYPSYDFA
                     HPLSDAIEGITHSLCTLEFEDHRPLYDWVVAEADTPHRPQQIEFARLNLTSTVMSKRK
                     LLELVGKKLVASWDDPRLPTLKGLRRRGVTPEAIRAFCEHIGVAKRDAIVEMQLLEHF
                     IREDLNKRSPRVMAVLRPLKVVLDNYPEGVVEELDAVNNPEDVSAGMRKIPFSRTLYI
                     EQDDFREDPPKQFYRL"
BASE COUNT          304 a          377 c          334 g          275 t
ORIGIN      
        1 tggtagaact ccaatctggg tctccaccca cacatgacca tgccaaggcg ttctcacaag
       61 atttcatgaa tacatcggaa ccctcaacct cttcgaattt cattcgggat ctcgtggcag
      121 ccgaccgggc ggtaggtaaa catggcggac gggtcgtcac gcgatttcct cctgagccca
      181 atggatatct acacatcggt cacgccaagt ctatcgtgct taattttggg atcgcgaacg
      241 agacccccgg aggagtctgc catctgagat tcgacgacac gaatcccacc accgaagacc
      301 ccgagtatgt tcaagccata caggaagacg tccgatggtt gggatttgat tggcacggca
      361 agatgttcta tgcatcggat tatttcgagc agctctacgc tttcgccatt gttttgatta
      421 agaaaggcaa agcctacgtt gatagcctca cggcggatca aatccgggaa caccgcggca
      481 cgctcaccga accaggccga gacagtcctt atcggacccg atcccccgag gagaacctgg
      541 atctctttgc tcgcatgcga gccggagagt ttgccgatgg aacccatgtc ttacgcgcca
      601 aaatcgatat gggctcccca aacatcaatc ttcgggatcc gatcctctat cggatccggc
      661 atgccaccca ctatcggaca gggaccgcat ggtgcatcta tccctcgtat gactttgcac
      721 acccgttgtc cgatgccatc gagggaatca cgcactccct ctgcacgctg gagtttgagg
      781 atcaccgccc cctctatgat tgggtcgttg ccgaagccga cacgccacat cgaccgcagc
      841 aaatcgaatt cgcccgactg aatctgactt ccaccgtgat gagtaagcgc aagctgctcg
      901 aattggtcgg caagaaactc gtggccagct gggatgatcc acgccttccc accttgaaag
      961 ggctccggcg ccgaggggtg actcctgaag cgattcgtgc cttttgcgaa catatcggcg
     1021 tcgcgaagcg cgatgcgatc gtcgagatgc agctgctcga acacttcatc cgagaggact
     1081 tgaacaaacg ctcgcctcgc gtgatggcgg tgctccgacc gttgaaagtt gtactcgaca
     1141 actatccgga aggtgtcgtt gaggaacttg acgccgtgaa taatcccgag gacgtctccg
     1201 cgggaatgag aaagattccc ttctcaagaa cgctgtacat tgagcaggat gatttcaggg
     1261 aggacccacc gaaacagttc taccgcctag
//
LOCUS       DASTWE010000146         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_520940, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000146 DASTWE010000000
VERSION     DASTWE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520940"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFT30_01635"
     CDS             <1..557
                     /locus_tag="VFT30_01635"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEU5091355.1"
                     /translation="FANRQVITHQIREAFTAVFGRSAEELGMELVYDVAHNIAKVERY
                     PEGELVVHRKGSTRALGPGSSDLPPRYRTTGQPVICGGSMETGSYLLAGTQKAVRDTF
                     ASTMHGSGRTMSRAKAKKTVRGEQIQKQMKQRGILVKAVSMSGLAEEAGFAYKNISEV
                     VETVDRAGITKPVAELRPIGNIKG"
     gene            897..1109
                     /locus_tag="VFT30_01640"
     CDS             897..1109
                     /locus_tag="VFT30_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091356.1"
                     /translation="MAVSKTYVADLCRRHQYRNLKARRKWKHHVPRPDPAQSDLGPRP
                     LTKNEKGQPSVVLTILDHASHAYLRL"
BASE COUNT          461 a          426 c          495 g          376 t
ORIGIN      
        1 ctttcgccaa ccgtcaagtc atcacccatc agatcagaga agcgtttacc gctgtattcg
       61 gccggtcggc agaagagctg ggtatggaac tggtctatga cgtcgcgcac aatatcgcca
      121 aagttgagcg gtatccggaa ggggaactgg tggtccatcg gaagggttca acgagagcat
      181 tggggcctgg aagttccgat ctcccgccac ggtatcgtac gactggtcaa ccggtcattt
      241 gtggcggttc aatggaaacc gggtcgtatc tgttggcagg gacgcaaaaa gcggtgcgtg
      301 acaccttcgc ttcgacgatg cacggatcgg ggcgcacgat gtcgagggcc aaggcgaaga
      361 aaacggtgcg tggtgaacag atacagaagc aaatgaaaca gcgagggatc cttgtgaagg
      421 ctgtctctat gtctgggttg gctgaggagg cgggattcgc ttataagaat atctccgagg
      481 tcgtcgaaac agttgatcgt gcgggaatca caaaaccagt agcggaactc cggccgatcg
      541 gaaacatcaa gggatgaaca cacaccctag cttggatact gtagttgagt ctgccgtggt
      601 gctggaaaaa ttcggacgat tgacgtcgaa ggattaagcc gctcttcgac aaattctcac
      661 cacacatttg tttatcgtga tcttattcgg gttatcctag cgtgcttcaa aggtatgtac
      721 agattccaag gcggcaacag gtccacggat gcgtgaaacc actgcctgct cgttcctcac
      781 ccaacccaag cctaaatggg tccagaacga tcatcaaact gaaagctctc atatcgcagg
      841 cgggctgccg gaccctggcc cattccttta accgtcgctg ggcatgccga aatcagatgg
      901 cggtcagtaa gacctatgtc gcagacctgt gtcgacggca tcagtatcgg aatctcaaag
      961 cgcgccggaa gtggaaacat cacgtgccac gacccgatcc cgcgcaatcg gatctggggc
     1021 cgcgaccgct gaccaagaat gagaaagggc agccgagcgt ggttttgacc atactcgatc
     1081 atgccagtca cgcctatctg cggttgtagc ggctgtccga tgaatcatcg tggatgttgc
     1141 tgtaggaact tatgaggcgg tcaaacgata cggccgacta caattcgtgc ggacagacaa
     1201 cgaagcggta tttaagtcct tctggcatgg gagttatgcc aacggctggt aacatgcagc
     1261 cgcgagtacg accataaact gacagggatt cgaatcttgt acagctatga tcggccgtac
     1321 gatcacttgt aagggcgaac actcgccgaa gtctgatccg ggatcaatat ctgcacggcg
     1381 aagttcggct aggaatacgg tacaaggggt agagagttat agagtcgtgc aacaccgacg
     1441 tctgtgagaa gtacggacgg gtctgttcgt aattgggcat gcatggaaaa aagaattaga
     1501 cacaaagaag ttcagccaga aaggaaaaat gtagcttagg ccgaggcagc acgggggatt
     1561 cggaagaaaa aatcaggtca cggatcgcag tgacatgtcc accggacagg acacccgaag
     1621 aactcgaatg tcaggaaact agtgcgttaa tcacgtgtct gtttttttcg gggccacttc
     1681 aaaccgccct catctttccc atttggtcca aaaactcaaa tttcagcttt tcagacaata
     1741 gtttgcgaat catttgcc
//
LOCUS       DASTWE010000147         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_876389, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000147 DASTWE010000000
VERSION     DASTWE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876389"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            475..1581
                     /locus_tag="VFT30_01645"
     CDS             475..1581
                     /locus_tag="VFT30_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEU5091357.1"
                     /translation="MYAREFRDGAKDGLEALEPLDPDKITSFSDLLEAMGKTAFGGRH
                     LGKAFDVLWAMIEDPDCHVVMTLSGAMTIAKMGKIITKMIDEGMVQCIISTGALMAHG
                     LSESVGKTHYRHDPSMSDEELFRKGYNRVYDTLEMEANLNYVEHVVAQTLKRLNHDRP
                     LSSEILTRELGKTLAEELDGDGILKSAYLKNVPVYIPAFTDSEVGLDMGTWAMAREVD
                     RARSHAGQGKDLDILRAIHQSCPSFNPYLDLNSYADHVLSAKRLGIFTIGGGVPRNWA
                     QQVGPYIEIGNLRLGLNVKPPRFHYGVRICPEPDYWGGLSGCTYQEGLSWGKFVTPKD
                     GGRFAEVLSDATVVWPLLMMGILERKKASAVHRS"
     gene            1578..>2156
                     /locus_tag="VFT30_01650"
     CDS             1578..>2156
                     /locus_tag="VFT30_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5091358.1"
                     /translation="MRKTVRQVLVQLFVLGGLGWISYVSAGDTLETDARMRGRATAPV
                     TLIEYSDFTCGYCLKFFKDTWPRIQARYVDTGKVRFFYKDYPRADRGSGVDAASAARC
                     AGDQGKYWGMHDRLFAAGGRLDKQVYLRHAVALALDQPAFERCMNDGRYTKAIFEDRQ
                     EANQWGFHGTPGFILVRTTAEPTEKEPAVAIPG"
BASE COUNT          496 a          511 c          644 g          505 t
ORIGIN      
        1 tggtcgtgtt tcatcggtgc tgccctatga ctccactgcg tgcaggacga tgcgaaagcc
       61 gcagccttgt caggaccctg cctataccta ggagtgcccg aattataggt cccgcctatc
      121 agaagggcta gagaaaggcc agagtaaaat tctgggtggc atcagggtcc atacgtcagc
      181 agacggcaga atccgtctat ccggaatggg caaaggacag atgtcacacg tatcaagcac
      241 ggataaatcg tcttgttgat ctgttcctat tcggactgca ctccgggccc actgccgatc
      301 cacttcctgc ggttgcatta cagcaactaa gtttgagttt ggaattcaaa agagggctca
      361 cgtctgactt ctgtgagctc atgcaatctg ccagacgcgg gaacctttca ttgacgcatc
      421 tgttctcggt tgtgtataaa caaatgcaga ttttagaatt tctcagagga tcgtatgtac
      481 gcacgcgagt ttcgtgacgg cgcaaaagac ggattggaag cgctcgaacc gcttgatccc
      541 gacaagatta cctcgttcag tgatttgctt gaggcaatgg gcaagactgc attcgggggg
      601 cgccatctcg gcaaggcgtt cgatgtgctc tgggcgatga tcgaggaccc tgattgccac
      661 gtcgtgatga cgttgtccgg tgcgatgacg atcgccaaga tgggcaagat catcacgaag
      721 atgatcgatg aaggaatggt gcaatgcatc atctcgaccg gagcgttgat ggcgcatggc
      781 ctcagtgagt cggtcggcaa gacgcattat cggcacgatc cttcgatgag cgacgaagag
      841 ctctttcgga aagggtacaa tcgtgtctac gacacactcg agatggaagc gaatttgaat
      901 tatgtcgagc acgtcgtcgc gcaaacgttg aagcgcctca atcatgatcg gccgctttcg
      961 tcagaaatcc tcacgcggga gttgggaaag acgttagccg aggaattaga cggagatgga
     1021 atcctcaaaa gcgcctactt gaaaaatgta ccggtctata ttccggcttt cactgattcg
     1081 gaagtggggc tggacatggg aacctgggcg atggctcggg aggtcgatcg agcccgatct
     1141 catgccgggc aggggaaaga tctggatatt ttacgagcga tccatcaatc atgtccttca
     1201 ttcaatccgt acctcgatct caacagctat gccgatcatg tcctctccgc taaacgactt
     1261 ggaattttca cgattggagg aggagttcca cggaactggg cgcaacaggt tggtccgtac
     1321 attgaaatcg gtaatcttcg attgggtcta aacgtgaagc cgcctcgatt tcactatggg
     1381 gtgaggattt gccccgaacc ggattactgg ggagggctca gtggctgtac gtatcaagaa
     1441 ggactttcct gggggaaatt tgtgacaccg aaggacggtg ggcgattcgc cgaagtattg
     1501 agcgatgcga cggtcgtctg gcccttgtta atgatgggga ttctggagcg aaagaaagcc
     1561 agtgctgtgc atcggtcatg aggaaaacgg tccggcaagt tctggttcag ctgtttgtcc
     1621 tcggtggact tggatggatc tcttacgtct cggccggaga tacgcttgaa accgatgctc
     1681 gcatgagagg gcgtgcgacc gcgccggtca cgctcatcga atattctgat tttacatgtg
     1741 gctattgcct gaagtttttt aaggatacgt ggccacggat tcaggctcgt tacgtcgata
     1801 ctgggaaggt ccgatttttt tacaaggatt atccgcgagc cgatcggggc tcaggagttg
     1861 atgctgcctc agcggcacga tgtgcgggtg accaaggaaa gtattggggg atgcatgacc
     1921 ggttgttcgc cgcaggaggc cggttggaca agcaggttta tctccggcat gcggtcgcgc
     1981 tcgccttgga ccagcccgcg tttgaacggt gtatgaacga cgggcggtat acgaaggcga
     2041 ttttcgaaga tcgccaggaa gccaatcagt gggggtttca tggaacccct gggttcatcc
     2101 tcgtgcgaac gaccgctgag ccgacggaaa aagagccggc cgttgcgatc cctgga
//
LOCUS       DASTWE010000148         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_68558, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000148 DASTWE010000000
VERSION     DASTWE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68558"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..398)
                     /locus_tag="VFT30_01655"
     CDS             complement(165..398)
                     /locus_tag="VFT30_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091359.1"
                     /translation="MRKGLVVLGLVFSACSFGTRVQPPPPVPALPEVSYVSPCDPKAV
                     AGLTQDAVETLRDRDLILRRHIQMLEQYVRGSQ"
     gene            504..>1146
                     /locus_tag="VFT30_01660"
     CDS             504..>1146
                     /locus_tag="VFT30_01660"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU5091360.1"
                     /translation="MKLAEIALRLGCELRGDGNIEIAGMNSIEDAQPHEMTFVANKKY
                     LTKLATTKAAAVILAPTDPDITLPSLRTPNPYLAFARVLEWFYPPYTPPVGIHPTAVI
                     ASTARVGASAYIGPYAVIDEHVVIGDHARIFAHVAIYPNVQIGHEFTAHAGAVVREGT
                     KIGDRVVLQAGVVIGGDGFGYVPLPDGSAYKIPQTGTVTLADDVEIGANTTIDR"
BASE COUNT          261 a          292 c          324 g          269 t
ORIGIN      
        1 agctacgcga actagttgtg attttccatc cggccttgcg aaataatgcg atagtagaat
       61 tctcaaggct gcctttggga ataccaagct ttaatgcatc catacgaagg aggttcgtaa
      121 cataggggcg ggggggtcgc aagttggact ctatgaagat tctgttactg actaccgcgc
      181 acatactgtt caagcatctg gatatggcgc cgcaggatga ggtcgcgatc gcgaagggtt
      241 tcgaccgcgt cttgcgtgag tccggcgaca gctttagggt cacagggtga gacatagctg
      301 acttctggta gggccggcac cggcggtggt ggctgaaccc ttgtgccgaa tgaacaagca
      361 gagaaaacta accctaagac aaccaacccc ttcctcatga tctcctcttt tacccgtgca
      421 ctcacatagt cgtgctagat agactctgct acatatggca cgcgtctcgc tttgacaaga
      481 cgcgcgaggc ggaggagagc acgatgaaac ttgctgagat tgccctacga ctcggatgtg
      541 agctgcgagg agacggaaat atcgagattg ccggaatgaa tagtatcgag gacgcgcagc
      601 cgcacgagat gacctttgtt gcgaataaga aatatctgac taagctggcg acgaccaaag
      661 ccgcggcagt cattcttgct ccgactgatc cggacatcac gctgccatcg ttgcggaccc
      721 ccaatcccta tcttgccttt gcgcgagtgc ttgaatggtt ttaccctccc tatacgccac
      781 cagttgggat tcatccgact gcggtgattg cgtccaccgc ccgcgtcggt gcgtcagcgt
      841 atatcggccc gtacgctgtc attgatgaac acgtcgtgat tggggatcac gcgcggattt
      901 ttgctcatgt ggcgatttat ccaaacgtcc agattggtca tgagtttacc gcacatgctg
      961 gtgccgtagt tcgcgagggg acaaaaattg gtgaccgcgt ggtcctgcaa gctggagtag
     1021 tgattggtgg tgacgggttc ggctatgtcc cgttgcccga cggatcagcc tataaaatcc
     1081 cacaaactgg gactgtcact cttgcagatg atgtcgagat cggtgcgaat accacgatcg
     1141 atcgtg
//
LOCUS       DASTWE010000149         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1134981, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000149 DASTWE010000000
VERSION     DASTWE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134981"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1013)
                     /locus_tag="VFT30_01665"
     CDS             complement(<1..>1013)
                     /locus_tag="VFT30_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5091361.1"
                     /translation="LSALGPGGLTRERAGFEVRDVHPSHYSRICPIETPEGPNIGLIT
                     SLATYARINQFGFIEAPYRKVVKGRVTDEIEFLSAIEGDKYIIAQANSKLDGGGRLVS
                     ETVSCRHGGDFVLATPDKIEYMDVSPKQVVSVATALVPFLEHDDANRALMGSNMQRQA
                     VPLVTSEAPLVGTGMEAVVARDSGYVIQARRAGVVESVDATRIVVRADSKDGKRGKDS
                     GLDVYDLIKFQRSNQNTCITQTPVVRIGQPVKKGQVLGDGPAIDHGELALGKNVLVAF
                     MPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGKEDVTRDIPNVGEEAL
                     RNL"
BASE COUNT          214 a          328 c          246 g          225 t
ORIGIN      
        1 ccaaatttct cagcgcctct tctccgacat tgggaatatc tcgcgtcaca tcttccttcc
       61 ccagcttggt atcccgggct tctacctcaa actcttcgat gtgaatcgag gtgaaagcgt
      121 cctcgcggac caatttctcg ctaagcaata tggcgtcctc gaagttgtat ccaccccagg
      181 gcatgaacgc cacgaggacg ttcttgccca gggcgagctc tccatgatca atcgccggtc
      241 catctccgag cacctgtccc ttcttaaccg gctggccaat ccgaacgacg ggggtctgag
      301 taatgcaggt attttgattc gatcgctgga atttgatcag gtcataaacg tccagcccgg
      361 aatccttccc cctcttcccg tcctttgaat cggcccgcac cacgatgcgg gtggcatcga
      421 cgctttccac gaccccggct cgacgagcct ggatgacata cccggaatct cgcgccacca
      481 ccgcctccat tccggtccca accagaggag cttctgacgt caccaaagga accgcctggc
      541 gctgcatgtt ggaacccatc agcgcacggt tggcatcgtc atgctccaga aacggtacga
      601 gcgcggtcgc aacactcacc acttgcttcg gcgacacgtc catgtactcg atcttatccg
      661 gagtggccag gacgaagtct ccaccatggc gacaggaaac ggtttccgat accagcctac
      721 cgcctccatc caatttcgag ttggcttggg cgatgatgta tttgtcaccc tcgatcgccg
      781 agagaaactc aatttcatca gtcacacgtc ccttgacgac tttccgatac ggcgcctcaa
      841 tgaatccgaa ctggttgatc cgtgcgtagg ttgccagcga tgtgatcaat ccaatattgg
      901 ggccttccgg cgtctcgatc gggcaaatac gactatagtg agacggatgt acgtctcgaa
      961 cttcgaaccc ggcccgctct cgcgtgagcc cccctggacc aagagccgac aga
//
LOCUS       DASTWE010000150         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1328871, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000150 DASTWE010000000
VERSION     DASTWE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328871"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..>1028)
                     /locus_tag="VFT30_01670"
     CDS             complement(49..>1028)
                     /locus_tag="VFT30_01670"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5091362.1"
                     /translation="GSLENLLHLQGNPEMADRLCTTIDTVLNVDILSELIGICDVVVH
                     LAAAVGVQYVLENPLSSILTNVQGTEKVLELCAKFRKKVLIASTSEVYGKHMHAPLIE
                     SDDCVYGPPTKSRWSYAASKLIDEFTALAYHRSRGLAVCIVRFFNTVGPRQTGRYGMV
                     IPRFVEQALSNRPITVYGTGRQTRTFTHVRDSCEAVMRLLDSPDAVGEVVNVGGIEEI
                     SMLELAHRIREKVGCNAQPQLVSYDHVFGHDFEDMQRRVPSTAKLRALTNFSPCTGLD
                     EILEDVISHYRANYHAGPRPLARANSKQATTDPFGDEIDSTCLTRLSQQ"
BASE COUNT          257 a          281 c          255 g          235 t
ORIGIN      
        1 aacaaatcaa ataaaactcc tcttatcctg gcatatcaga acatctgttc actgctggga
       61 gagtcgcgtc aaacaggtgc tatcaatttc gtcgccgaac ggatcggtgg tggcctgttt
      121 ggaatttgct cgcgccaatg gccgcgggcc agcatgataa ttcgcacgat agtggcttat
      181 gacatcctct aggatttcat ccagtccggt acaaggggag aaattggtca acgcccgcaa
      241 cttagcagta gacggcacgc gcctctgcat atcttcaaag tcatgaccga agacatgatc
      301 ataagagacg agttgaggtt gtgcattgca tcccactttt tccctgatgc gatgcgcgag
      361 ttcgagcatg gatatctctt caattcctcc cacattgaca acttccccta ccgcatccgg
      421 cgaatccagc agacgcatga ccgcttcaca ggagtcgcgc acatgtgtaa acgtcctcgt
      481 ttgacgacca gttccataaa cggtaatcgg gcgattgctc agagcctgct ccacaaatcg
      541 tgggattacc atgccgtacc gtccggtctg gcgaggaccc accgtattaa aaaacctgac
      601 aatacagact gccaaacccc ggctccgatg ataggccaaa gcggtgaact catcgatgag
      661 cttcgaagcg gcatagctcc agcgtgattt cgtgggagga ccatagacac aatcgtcgct
      721 ctcgattagc ggagcatgca tgtgcttccc atacacttcc gatgtggacg caatgagaac
      781 cttcttgcgg aacttggcac aaagttcgag cactttctcg gtgccctgca cgtttgtcag
      841 gattgaggag aggggattct cgagcacata ctgcaccccg actgcggcag caaggtgaac
      901 gaccacgtcg catattccga ttaactcgga taggatgtcc acattcagca ctgtatcaat
      961 ggtcgtacac agcctatctg ccatctcggg attgccctgc agatgaagga gattttcgag
     1021 agacccgg
//
LOCUS       DASTWE010000151         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1328876, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000151 DASTWE010000000
VERSION     DASTWE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328876"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="VFT30_01675"
     CDS             <1..>1007
                     /locus_tag="VFT30_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5091363.1"
                     /translation="QGAATGVGGILRDIFTMGARPIALLDSLRFGELHSPKNRHLMKG
                     VVSGIAGYGNCMGVPTVGGEIVFNDIYSLNPLVNVFCLGLAHQDKIFRGTAAGVGNPV
                     IYFGSKTGRDGIHGATMASDSFDDQSEQKRPTVQVGDPFTEKLLLEACLELMERDLLV
                     GIQDMGAAGLTSSSCEMASRAGNGIELDLTVVPRREPGMTPYEIMLSESQERMLMVAK
                     AGKEDECIEICRKWDLDVAVVGKVTADGILRVLDQGKVVAEIPAKALADDGPRYERPF
                     QPPAYQDMLTNLNYDAIPDVKDANVALLTLLESPTIASKRWVYEQYDHMVRTNTTVRP
                     G"
BASE COUNT          221 a          255 c          310 g          221 t
ORIGIN      
        1 atcaaggcgc tgccacggga gtaggcggca ttctgcgcga tatctttacc atgggggcga
       61 ggccgatcgc gctccttgat tcactgcggt tcggagaatt gcactcgccg aagaatcgcc
      121 atctcatgaa gggtgtggtc tccggcatcg caggctacgg caattgcatg ggggtcccga
      181 ccgtaggagg cgagatcgtc ttcaatgaca tctactctct taatccgttg gtcaatgtgt
      241 tttgtctcgg tctcgcccat caagacaaga tattccgtgg cacggccgct ggagtcggga
      301 atcccgtgat ctatttcggt tcaaagacgg gccgcgatgg gattcacgga gcgacgatgg
      361 cgtccgattc gttcgatgat caatcggagc agaagcggcc gaccgtccaa gttggtgatc
      421 cctttacgga aaaattgctg ctcgaagcct gtcttgagct gatggaacgc gatctcctcg
      481 tcggaattca agacatgggt gctgcggggc tgacgagttc ttcctgcgaa atggcctctc
      541 gcgccggcaa cgggatcgag ttggacctga cggtggtacc acggcgcgag cccggtatga
      601 cgccctatga gatcatgcta tccgaatctc aagagcgtat gttgatggta gcgaaagccg
      661 gcaaggaaga cgaatgcatc gaaatttgcc ggaagtggga tctggacgtt gccgtggtcg
      721 ggaaagtgac cgccgacgga atcttgcgtg tcctggatca gggaaaggtt gttgcagaaa
      781 ttccggcgaa ggcattggct gatgacggcc cgcgttatga acgacccttt cagccaccag
      841 cctatcaaga tatgctgacg aacctgaact atgacgccat tcccgatgtg aaagatgcca
      901 atgtggcatt gctgactttg ttggagtcgc cgacgatcgc cagcaagcgg tgggtatacg
      961 aacagtacga tcacatggtg cggacgaata ccacggtgcg acccggt
//
LOCUS       DASTWE010000152         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_230303, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000152 DASTWE010000000
VERSION     DASTWE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230303"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1258)
                     /locus_tag="VFT30_01680"
     CDS             complement(<1..>1258)
                     /locus_tag="VFT30_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU5091364.1"
                     /translation="YVPESLLTKMEGIGRQYTAYKQIKAMKNPIKAGAYLFVAVVAVL
                     ILFSATWFGFYVARGITVPIQRLAEATEAVAQGDLSVQIDAKATDEIGTLIESFNRMT
                     QDLQGSKSKLEEANLTLRNTNVELDRRRAYIETVVDTIAAGLLSIDRSGTITTFNPSA
                     ERILGLAADRFRGRSANEVFKEFGLDSFQTAYDRMLADERDDLDLEAQLDIQGKLITI
                     GLKGSRMRDEANKDLGFVLVFEDLTELIKAQKVAAWQEVAKRVAHEIKNPLTPIQLSA
                     QRLRKKFFEQSPDLDRVFDDATNVIINEVGSLKEMLDEFSKFARLPAPQMTRQSLHDV
                     VREVATLYREAQKDIELIVDLDEDLPSINFDREQLRRVFVNLFDNAVQAMNQKGRLWV
                     DTKYDTKRRRAVVTVADEGPGIAPEDQ"
BASE COUNT          274 a          380 c          311 g          293 t
ORIGIN      
        1 ctgatcctct ggagcgatgc caggcccctc atccgctacc gtgacgactg cgcggcggcg
       61 tttggtatca tacttcgtat cgacccacag ccgtcctttc tgattcatcg cttgaaccgc
      121 gttgtcgaag agattgacga acacacgcct caattgttcc cggtcgaaat tgatggacgg
      181 cagatcctcg tccaggtcca caatcagctc aatatccttc tgcgcttcgc gatagagggt
      241 ggccacttcc cgtaccacat cgtgcaagga ttgccgcgtc atttgcggag ctggcaggcg
      301 ggcgaatttt gagaactcgt ccagcatctc tttaaggctt ccgacttcat tgatgatcac
      361 attggtggca tcgtcgaaca cccggtcaag gtcaggagac tgttcgaaga acttcttgcg
      421 caatcgttga gcggataatt gaataggagt cagtggattc ttgatttcat gggcgacacg
      481 tttcgcgacc tcctgccaag ccgcaacctt ctgcgccttg atcaactccg tcaaatcttc
      541 aaagaccaac acaaacccca agtctttgtt cgcctcatcc cgcatacgag aaccttttag
      601 accgatcgtg atcagcttcc cttggatatc caactgtgct tccagatcca aatcgtcgcg
      661 ctcatcggcc agcatacggt cataggcggt ctgaaacgag tcgaggccga actccttgaa
      721 cacctcattg gccgatcgac ctctgaaccg gtcggcagcc aacccaagga tgcgctcggc
      781 cgatgggttg aaggtggtga tcgttccgct ccgatcgatc gataacaggc cggcggcgat
      841 cgtatcaacg accgtttcga tataggcgcg gcggcgatcc agctcgacat tggtgttccg
      901 cagagtcagg ttggcctcct caagcttcga tttactcccc tgcagatcct gcgtcatccg
      961 attgaacgac tcgatcagcg tcccaatttc atcggtcgcc ttggcatcga tctgcaccga
     1021 cagatccccc tgggcgacag cttcagtcgc ttccgccaac cgttgaatcg gaacggtaat
     1081 acctcgcgcc acatagaacc cgaaccacgt cgcgctgaag agaatcaaca ccgccacgac
     1141 ggcaacaaaa aggtacgccc ccgctttaat ggggtttttc atcgccttga tctgcttgta
     1201 cgccgtgtat tgacggccaa tcccttccat cttggtcaac agggattcag ggacgtag
//
LOCUS       DASTWE010000153         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_715010, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000153 DASTWE010000000
VERSION     DASTWE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715010"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..549
                     /gene="rplS"
                     /locus_tag="VFT30_01685"
     CDS             145..549
                     /gene="rplS"
                     /locus_tag="VFT30_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5091365.1"
                     /translation="MNQLERIQRSLTKKSVPNFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGSLNTEMFTVRKLSYGVGVERIFPVHSPIVSKVDVVRQGRVRRAKLYYLRGK
                     KGRFAKVEEREFVGESKPAAQPTASEEEAVTA"
     gene            591..1271
                     /locus_tag="VFT30_01690"
     CDS             591..1271
                     /locus_tag="VFT30_01690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5091366.1"
                     /translation="MIGEGMCDGPTEEFERAARSCGYRRIAGIDEAGRGPLAGPVVAA
                     AVVLPTRCRLSGVDDSKQLSEGERDRLYAAILEQAVGVGVGSADVGEIDRLNILEATR
                     LAMRRAIDQLAPSPDYLLIDAVALPGIRISVRPIIKGDSLSLSIAAASIIAKVTRDRL
                     MAMYQDMFPEYDFLSHKGYCTTGHLQRLARHGPCSIHRRTFMPVQEVIIAGKNDSARR
                     MSDTLFEQ"
     gene            1276..1665
                     /locus_tag="VFT30_01695"
     CDS             1276..1665
                     /locus_tag="VFT30_01695"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5091367.1"
                     /translation="MATSDPRHQFGQASEMRAEQFLLAKGYRILDRNVRTPLGELDLV
                     AEDQGVVVFVEVKGRATEAFGGALLAVDQRKRVKLTKLAAQYLARRHWSDKTCRFDVV
                     LVQGRPSDQGRIKHLQNAFDISEHGEF"
     gene            1687..2376
                     /gene="ftsE"
                     /locus_tag="VFT30_01700"
     CDS             1687..2376
                     /gene="ftsE"
                     /locus_tag="VFT30_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5091368.1"
                     /translation="MDAIIQLIHVSKWHDRRAALSDVTLEIEKGEFVLLMGSSGAGKS
                     TLLRMLIGEEQPDEGQIFVHGRNVTKLRRTEIPFLRRKVGAVFQDFRLLPKKSVFDNV
                     ALPLIVQGASEKDIRRKVTEALRAVDVDHKKDQSPNSLSTGEQQRVCIARAIVNGPVV
                     LLADEPTGNLDPERTREIIELFKLINARGTTVIVATHDPHVLNHVNRRVITLVQGVLL
                     SERKIGQGIGV"
     gene            2373..>2795
                     /locus_tag="VFT30_01705"
     CDS             2373..>2795
                     /locus_tag="VFT30_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU5091369.1"
                     /translation="MRRLFYLVREAWANMRINRTTTIVAVLTTAFTLACVGIFLLLYV
                     NLRNAAGSLQEDVKVMVYLEDRLPRERMQELERRLKIDRMVAEVLFISKEQALGEFRV
                     QFPSESHLLEGLGENPLPASFVVTLAPNFRSPDAMKGWV"
BASE COUNT          624 a          681 c          849 g          641 t
ORIGIN      
        1 aagcattgcg aaacacgtat ttgcggcgcc ccgatcttct gaaagatcgg gtgtttacga
       61 aggaagatca acagttgttg gatgaattga tgagcgaagg gcttttgacg gccccgacat
      121 cacgccggga ggagggttaa gtccatgaat cagttggagc gaattcagcg gtcgttaacg
      181 aagaaatcag tgccgaactt tgagattggg gataccgtca gggtccacgt caaagtcgtc
      241 gaaggcgaga aagagcgtat tcaggtttat gaaggcaccg tgattgcgcg caaggggagt
      301 ttgaataccg aaatgtttac ggtccgaaaa ctttcatacg gggtcggggt cgaacggatt
      361 ttcccggtgc attctccgat cgtctccaag gttgatgtgg ttcggcaggg acgagttcga
      421 cgcgcgaagc tctactattt gcgcggcaaa aagggacggt tcgcaaaggt ggaagagcgc
      481 gaatttgtcg gagagagcaa gccggcggca caaccgacgg cttctgagga ggaggctgtg
      541 acggcctaga cggtttccac ctatgtgttc cctcacggca atgcgatggc gtgatcggtg
      601 aagggatgtg tgatggaccc actgaagagt tcgagcgggc ggcccgctcg tgtgggtatc
      661 gtcgtattgc cggtatcgat gaagccggac gcggtccatt agccggtccg gtcgtcgccg
      721 ccgctgttgt cttaccgacc cgatgtcgac tttcaggggt cgatgattct aaacagcttt
      781 ctgaaggaga gcgagaccga ttatatgccg cgattcttga gcaagccgtg ggtgtgggag
      841 ttggatcggc ggacgtaggt gaaatcgatc ggctcaatat tctcgaagca acccggttag
      901 cgatgcggcg agccatcgat caattggctc cctcccctga ttatctactc attgatgccg
      961 ttgctcttcc tggaatcaga atctccgtac ggcctatcat caagggggac tccctttccc
     1021 tttcgattgc ggctgcctcc atcatcgcca aagtcacgag ggatcgactc atggcgatgt
     1081 accaggacat gttccccgaa tatgatttcc tctcgcataa ggggtattgt acgacaggac
     1141 atctgcaacg attggcgcgc catggtccat gctccatcca tcgtcgaacg ttcatgccgg
     1201 tgcaagaggt gattatcgcc ggaaagaatg attcggcccg acggatgagt gacacattgt
     1261 tcgaacagta agcgcatggc cacttccgac ccgcgccatc agttcggcca agccagcgaa
     1321 atgcgggccg aacagttctt gttggccaag ggctatcgaa ttctcgatcg caacgtacgg
     1381 acccctctcg gagaattgga cctcgtggca gaagaccaag gtgttgtggt ctttgtcgag
     1441 gtcaagggca gggctacgga agccttcggt ggtgctctgc tggcggttga tcaacgcaag
     1501 cgggtgaaac tgacaaaatt ggctgcgcag tatttggcgc gacggcattg gtccgataag
     1561 acctgccgat tcgatgtggt gttggttcag gggagacctt ccgatcaggg aaggatcaag
     1621 catctccaaa acgcgtttga catcagtgaa cacggagaat tctagccctt ctccagcggg
     1681 gtttccatgg atgccatcat tcaactcatc catgtgtcca aatggcatga tcggagagcc
     1741 gcgctgtccg atgttacgct cgagatcgaa aagggggagt ttgtcctcct catgggatcg
     1801 agcggggccg ggaagtctac cctcctgcgt atgctgatcg gtgaggagca gccggacgaa
     1861 ggacagattt tcgttcacgg caggaatgtg acgaagctca gacggactga gatccccttt
     1921 cttcggcgaa aggtcggcgc ggtgtttcaa gactttcgtc tcttgccaaa gaaatcggtc
     1981 ttcgacaatg tggcgctccc attgatcgtt caaggtgcat ccgaaaaaga tatccgtcgt
     2041 aaagtcacag aggcattgcg tgcggtcgat gtcgatcata agaaagatca gtcgccgaac
     2101 agtctttcaa cgggagagca gcagcgtgtg tgtattgcgc gggcgatcgt caatgggccg
     2161 gtcgtgctcc tggccgacga accaacgggt aatcttgatc cggaacgtac cagagagatc
     2221 attgagttgt tcaaattgat caatgcccga gggacgaccg tgatcgtcgc gacgcacgat
     2281 cctcatgtct tgaatcacgt gaatcgacgg gtgattacct tggtgcaagg agtgctgctg
     2341 tcggagcgga agatcgggca agggatcggg gtatgaggcg actattttat ctcgtgcgcg
     2401 aagcgtgggc gaacatgcgg atcaatcgca cgacgacgat cgtggccgtt ctgaccaccg
     2461 cgtttacttt ggcctgtgtc ggcatctttc tgttgctcta tgtgaatctg cggaacgccg
     2521 cggggtcgct ccaggaagat gtcaaggtca tggtctattt ggaggatcga cttcccagag
     2581 aacggatgca ggaactcgag cgaagactca agatcgatcg catggttgcc gaggtgctct
     2641 ttatttcgaa agaacaggcg ttgggagaat ttcgagtcca attccccagt gagtctcact
     2701 tactcgaggg gcttggcgaa aacccccttc cagcctcgtt tgtggtgacg cttgcaccga
     2761 actttcggtc tccggacgcc atgaaaggct gggtt
//
LOCUS       DASTWE010000154         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1296656, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000154 DASTWE010000000
VERSION     DASTWE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296656"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFT30_01710"
     CDS             complement(<1..720)
                     /locus_tag="VFT30_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5091370.1"
                     /translation="MALSTSVHDLRTLIRSSHPLVVIETVEEERVLALLQSVTAQERM
                     PLFEWSVTRGLSRADEGSTLSNKMTATPLAVLQHLHGLTVEAVFWLKDLGPHLQDPAV
                     CRQLREVAAIYSRSRATCLLTGQPITLPLDLDKIAVRLDLKLPDRDELRSMLRGLLQS
                     LGPRTTIRPRSTTLAQNILHSISETKTHENGPSAKESDTILRALQGLTLHQARQVIAH
                     CIVERGTLSVEDVQTILKRKVQ"
     gene            complement(795..>1099)
                     /locus_tag="VFT30_01715"
     CDS             complement(795..>1099)
                     /locus_tag="VFT30_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091371.1"
                     /translation="ALARNGYPLKVSVKTDQRAVYVTERKISNPTSLEVTGSRNQYLI
                     FLQALPDNLTKLGVKAEFERVAQGGRILSVSHEETNEIEHRLINEINKALANPAKT"
BASE COUNT          221 a          281 c          339 g          258 t
ORIGIN      
        1 ctgtactttc cgctttagaa ttgtttgaac atcctcgaca gagagcgtgc cgcgttccac
       61 gatacagtgg gcgatgacct ggcgggcttg atggagcgtc aagccctgga gagcacggag
      121 aattgtgtcg gactccttag ccgaggggcc gttctcgtgt gtcttcgttt cgctgatcga
      181 atggagaatg ttttgagcga gagtcgtaga ccgtggacga atggttgttc gaggtcccaa
      241 cgattggagc aagccgcgca gcatcgaccg cagttcgtct cgatccggca gcttcagatc
      301 gagtcgtacc gcaattttgt cgaggtcaag cggcaacgtg atcggttgac ccgtcaacaa
      361 acaggtggct cgggatcggc tgtagattgc agcaacctcc cgaagttgtc ggcagacggc
      421 gggatcttgc agatgtggcc caagatcctt gagccaaaac accgcttcta ctgtcaggcc
      481 atgaagatgc tgaaggacgg ccaaaggcgt cgcggtcatc ttattactga gtgttgagcc
      541 ttcgtcggcg cggctaagcc ctctggtgac ggaccattcg aagagcggca tgcgctcctg
      601 tgcggttacc gattgtagta aggccaggac tcgctcctcc tccaccgttt caatgacgac
      661 gagaggatgg gaggagcgga tcagggtgcg aagatcgtgc acgctggtcg agagtgccat
      721 cggaaggaaa tgctagcacg aggcctcgac cggaccaaga aaaccggtga ttgtgaccac
      781 cgtgtgccga cgatttatgt cttggctgga ttggcgagcg ccttgttgat ttcgttgatc
      841 aggcgatgct cgatttcgtt cgtttcttcg tggctgacgc ttaagatgcg gcccccctgt
      901 gcgacccgtt cgaattccgc tttcaccccg agtttcgtca gattgtcggg aagggcttga
      961 aggaaaatga gatattgatt ccgcgagcct gtcacttcga gcgacgtggg gttggagatc
     1021 ttgcgctccg tcacatagac cgcccgttga tcggtcttga cactgacctt cagcggataa
     1081 ccgttgcgtg ccagtgcgt
//
LOCUS       DASTWE010000155         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1458298, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000155 DASTWE010000000
VERSION     DASTWE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458298"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFT30_01720"
     CDS             <1..764
                     /locus_tag="VFT30_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091372.1"
                     /translation="DPGLQSYLVTVAMKIHRTQSVESRMAAKSSTHFIVKFDGEEDQN
                     TWISVLEILEEAYREIGQKFGHFPSKPIIVVLHAKDSFQGATGSPAWADGLYDPTLGR
                     IQIPTQGATTDRKWLASVLRHEYVHALLHDRLGTSSGALPTWLNEGLAMQLAGDAWPE
                     LDEAMSGDIKVIPLTYLEGSWGALPASTSMMAYLEANSATRYLIERWGMARVNELLNA
                     LQTKSSVAAALQNRLFVSYEQFHQQWLESFERSRS"
     gene            complement(811..1689)
                     /locus_tag="VFT30_01725"
     CDS             complement(811..1689)
                     /locus_tag="VFT30_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091373.1"
                     /translation="MLHGCRWNVLPSFSRQAHVTDQRQSPTCHDSSKILRAFVAVVLC
                     LAFSIGQAWGEIGTEDKRAIVAAGFGYQIGTVSTIAVKVYDADSGDILSNETYELAVK
                     ESDGVRSNRGPRIFAGGVGLGATDLSNFVLRVYDANTGTFQWEGRLNLVQPDWRAGGK
                     VVSTVLPRQATVTRVQVVEATMEQPVFLLRAMDAATGMLVWEDEFTTVRTRIPLAQQI
                     AGQSIQPTSTLPASSHTFNFRIRMYDPSGRKVLWEDQLSQLDSDEGPEQSHSDQADML
                     PAWPHLPQEESAAGSI"
     gene            complement(2592..3659)
                     /locus_tag="VFT30_01730"
     CDS             complement(2592..3659)
                     /locus_tag="VFT30_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU5091374.1"
                     /translation="MTQRITTFDEFRDAVSIYRLPRVLLAALELDLFTTVGDRSWTIP
                     HLAKELKVSERGLSILCRNLAAVGVLQKKGASYWNSRLGATALNADHRAYRGGYLDLI
                     KRHWADWVRLLESVRSGLPIDHDTPDSPDYRRQFTWAMHHRTLEIAPAIAAQVPLRRF
                     KTLLDLGGGPGTYAMAFLAKNPTLHATVGDRAPALEVAKEIASTHKARRRLSYLPLDF
                     SNEPIPGTYDVIWYSNVLHIYSPEENQAIFRRALAALTPGGRFIIQDAFLHDREGLYP
                     IEASLFAVSMLLFTEGGNTYSASETATWLKNAGFVAVKSLPIKKGTEDWEDGILEASA
                     PGPRPKATVHRTRSSRDRKVR"
     gene            complement(3656..>4041)
                     /locus_tag="VFT30_01735"
     CDS             complement(3656..>4041)
                     /locus_tag="VFT30_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091375.1"
                     /translation="SFPQFYELEPSGPLLMDLGVDGWLLEVRPDGKLLCQYGVAMDEV
                     IALMSEGTPEDLGTDEVAKQAKYFLQPAVAKYRALLLQSGFVEETEMTDEFVAVTFAR
                     NADLQNRAKLEDLLRWCRRQIGSAS"
BASE COUNT          934 a         1112 c         1052 g          943 t
ORIGIN      
        1 gggatcctgg attgcagtcc tatctcgtca ccgtcgcgat gaagattcat cgcactcagt
       61 cggttgaatc tcgcatggct gcaaaaagca gtactcattt tatcgtgaaa ttcgacggtg
      121 aggaagacca gaatacctgg atctcagtgc tggagattct tgaagaagct tatagagaaa
      181 tcggacaaaa gttcggtcat tttccctcga aaccgatcat agtcgtcctc catgccaagg
      241 attcgttcca aggagcgacc gggagtccgg cctgggcaga tggcctctat gaccccactc
      301 ttgggcgtat tcagattcct actcaagggg cgaccacgga tcggaagtgg ttagcgagcg
      361 tgctccgtca cgaatatgtc catgccctcc ttcatgaccg cttaggaacg agcagcgggg
      421 cgctgccgac ctggttaaat gaaggtctgg ccatgcaatt ggccggagat gcgtggccag
      481 agcttgatga ggccatgagc ggagacatca aagtcattcc cttgacttat ctggaaggct
      541 cgtggggcgc actgccggct agcacgtcga tgatggccta cttggaggct aattcggcaa
      601 cacgctacct aattgaaaga tgggggatgg cccgagtcaa tgagttgctg aatgctttac
      661 aaactaagtc gtcggtagca gctgcacttc aaaacaggtt gtttgtctcc tacgagcaat
      721 tccaccagca gtggctcgaa agcttcgaac gaagccggtc gtaaccttgt cggcttcctt
      781 gcgggtcatt ggccagggat actagcgtca ctagattgat cctgctgcgg actcttcctg
      841 cggcagatga ggccaggcag gaagcatgtc ggcctgatcg ctgtgcgact gctcaggccc
      901 ttcatccgaa tccagctgag agagctgatc ctcccacaga actttccttc cgctgggatc
      961 atacatgcga attcggaagt taaaggtgtg ggaactcgca ggtagagtgc tggtcggctg
     1021 aatcgattga cctgcaatct gctgggccag cggaattcgt gtacggacgg tcgtaaactc
     1081 atcctcccat acaagcatgc cggtcgctgc atccattgcg cgcagcaaaa acaccggctg
     1141 ctccatggtc gcttcaacaa cctgtaccct cgtaaccgtg gcttgccgcg gcagcacggt
     1201 cgatacgacc tttccccctg ccctccagtc aggctgcacc agattgagtc gcccttccca
     1261 ttggaatgtt ccagtatttg cgtcgtatac ccgcaacacg aaattggaga gatctgtagc
     1321 gccaagtccg actccaccgg caaaaatacg cggcccgcgg tttgatctca caccgtcact
     1381 ttctttgacg gctagctcat aggtttcatt ggacaagatg tcacccgagt cagcgtcata
     1441 cacctttacc gcgatcgttg agacggtacc gatttggtaa ccgaacccgg cggccacaat
     1501 cgcccttttg tcttctgtgc cgatttcgcc ccaggcctga ccaatagaga atgcgaggca
     1561 caagacaact gcgacgaatg ctctcaggat cttcgaggag tcatggcaag taggactctg
     1621 ccgttgatca gtcacatggg cttgacgaga aaaactcggc aacacgttcc atctacatcc
     1681 atgcaacata tatccctcct ctacagttga acgcattctg gaagaggata tgtcgcccgt
     1741 aaattactcg ctgtaggaag aatcccctcg aaagaggggg atctcacaga agataaggaa
     1801 ccgtttacgt cagtcttgtc gactagagag ttcggcgtcg ccaaaccatc gataccgatg
     1861 gcgcgcaacc atgcgatagc cccattgagc tagtcgcttt gcagagggga accgtaacaa
     1921 ccacgataga agcttcccac ctgggaggag agggacgaga gggagaaacg cttcgagacc
     1981 ccgaattact tctcctgaag gttgaatcaa gaacgccaca tcaggccgac caggacgata
     2041 attcgcccct aacattctct ttgccgtttc gctctgatac gcaaggaacc taacgtcgga
     2101 ttctgtttgc ccaattccta gctcttcgag tttctgtttt gcggacacac acaatcggca
     2161 ttctccatca tagaccagta ggcagacttg cgttgatact ggcccatggc tctgcttctt
     2221 gaactccatg gtacaggtag gttaacaccc tgaaacggca ggcaacaagc aaccattgcc
     2281 gacgttttcc tccgcggtct atagttttca gatgaaacaa accgtcttgc tgagtctgtt
     2341 gattggcgtg cttgtcgtcg agataggatt ttacctaata ggcgcatcca caacccatgg
     2401 catggcttgg agaatcggtt ttttcatcct catgcctctg gcgctcgcgg tattgatttg
     2461 gctccgcctc cgatgggccg ccatggtctg cgtgatctat gccacggttg gattggcact
     2521 ggacatagca acaatcattc aggtgctgac aaaagattca gagttgggag tttccttggt
     2581 agccaatgcg gtcagcggac ttttctatct ctgcttgatc gtgttcggtg gacggtcgct
     2641 tttggacgtg ggcccggagc cgatgcctct agaatcccgt cctcccaatc ctccgtcccc
     2701 ttcttgatgg gaagcgattt gacggcgacg aatccggcat ttttgagcca cgtggccgtc
     2761 tccgaggctg aataggtgtt tccgccttcc gtaaacagta acatcgacac agcaaacaaa
     2821 cttgcttcta ttgggtacaa accttcgcga tcgtggagaa acgcgtcttg gatgatgaat
     2881 cgaccaccgg gcgttaatgc ggccaaggcc cgacgaaaga tggcctggtt ctcttcgggc
     2941 gaataaatgt gcagcacatt cgagtaccag atcacatcat acgtaccagg gataggttcg
     3001 ttggagaaat cgagcggtag ataggagagt cgccgccgcg ctttatgagt actggcaatt
     3061 tctttggcga cctcaagagc gggcgcccga tcaccgacgg tcgcgtgaag cgttgggttt
     3121 ttcgcgagaa atgccatagc gtaggtgcct ggccctccac cgagatccag cagtgttttg
     3181 aaacgtctta aaggaacctg ggccgcaatc gccggggcaa tttccaatgt tcggtggtgc
     3241 atggcccacg tgaattgtcg acggtagtcc ggactgtctg gagtatcatg atcgatcggc
     3301 aagccactcc tcacggattc caacagtcgt acccagtccg cccaatggcg tttgatcaaa
     3361 tccaaatatc cgccgcgata tgcgcgatgg tcggcattca gcgctgttgc tcccagccga
     3421 ctattccaat agctggctcc ttttttctgc aataccccga cggctgcgag attacgacac
     3481 aggatgctca aacctctttc gctgaccttg agctccttgg caaggtgggg gatcgtccac
     3541 gatcggtcac cgacagtcgt aaagagatcc agttccagcg ctgccaacaa tactcgtggt
     3601 aatcggtaga ttgagacagc atcacgaaat tcgtcgaatg tcgtgattcg ttgagtcacg
     3661 atgcgcttcc gatttgcctg cggcaccatc ggaggagatc ttccagcttg gcgcgattct
     3721 gcagatccgc atttcgcgca aacgtaacgg ccacaaactc gtcggtcatc tcggtctcct
     3781 caacaaaccc ggattgaagg aggagcgccc gatatttggc aacagccggc tgaagaaagt
     3841 acttggcttg cttcgcgact tcatctgttc ctaaatcctc tggcgttccc tccgacataa
     3901 gcgcgataac ttcatccatc gccaccccat actggcaaag gagcttccca tccggcctga
     3961 cctctaagag ccagccatct actccaagat ccatcaagag cggaccgctg ggctccaatt
     4021 cgtaaaactg agggaaagac c
//
LOCUS       DASTWE010000156         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_650486, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000156 DASTWE010000000
VERSION     DASTWE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650486"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFT30_01740"
     CDS             <1..599
                     /locus_tag="VFT30_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEU5091376.1"
                     /translation="DYVGKGLSGGRIVVYPPKASTFQAHENVIIGNVAFYGATSGEAY
                     ISGIAGERFCVRNSGVLAVVEGVGDHGCEYMTGGRVVVLGPTGRNFAAGMSGGIAYVL
                     DEKGDFIVRCNRAMVEVTGIDDASEIELVKNMIFRHAEYTASRRATEILLSWDEMISR
                     FVRVLPHDYKRVLEGAKGTANFALPIVEIRVKSADVSG"
     gene            629..>1055
                     /gene="gltD"
                     /locus_tag="VFT30_01745"
     CDS             629..>1055
                     /gene="gltD"
                     /locus_tag="VFT30_01745"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEU5091377.1"
                     /translation="MGKPTGFIEYARSVPLVRPALERVTDWREFHGHSDDTELQNQGA
                     RCMDCGVPFCHTGNVLGGAASGCPINNLIPEWNDLVYRGRWREAFDRLRKTNDFPEFT
                     GRVCPAPCEGSCVLGVNDNPVTIKSIEQAIIDRAFDAGWV"
BASE COUNT          239 a          231 c          337 g          248 t
ORIGIN      
        1 acgactacgt ggggaaggga ctttcgggag gaaggattgt cgtctatccg cctaaggctt
       61 caacttttca ggcgcacgag aacgtgatta ttggcaacgt cgcattctat ggggctacga
      121 gcggagaagc atatatatcc ggtatcgccg gtgagagatt ttgcgttagg aacagcggtg
      181 tgttggctgt ggtcgaagga gtcggagacc acgggtgtga atacatgact ggtggtcgcg
      241 tggtggtgct tggtccaact ggccgcaact tcgctgccgg catgtccggc ggaatcgctt
      301 acgtgctgga tgagaagggt gacttcattg ttcgatgcaa tcgggccatg gtggaggtaa
      361 cgggcatcga cgacgcgagc gaaatcgaat tagtcaaaaa catgatcttt cggcacgcag
      421 agtacaccgc tagccggcgg gcgactgaga tattgttgtc gtgggatgaa atgattagca
      481 ggtttgttcg ggttctgcca cacgattaca agcgagtgct cgagggggca aagggaaccg
      541 cgaattttgc actgccgatt gtagagataa gagtcaaatc ggcggatgtc tctggatagg
      601 ccactccgaa acagaatggt gtaagccgat gggcaaacca acaggattta tagagtacgc
      661 gcggtccgtt ccattggtgc ggccggcttt ggagcgggta acagactggc gtgaattcca
      721 tggtcactcg gatgacactg aacttcaaaa tcagggtgcg cgctgcatgg attgtggcgt
      781 gccattttgc catacgggaa atgtgttggg tggcgctgcc tcgggttgtc ctatcaataa
      841 cttaatcccg gaatggaatg atctggtcta tcgcgggcgc tggcgtgaag ctttcgatcg
      901 acttcgtaaa accaacgact ttcccgagtt cacgggcaga gtttgccctg cgccatgcga
      961 gggctcatgc gtgctcggtg tcaacgataa tccggtgaca atcaagagca ttgaacaggc
     1021 gatcattgat cgcgcctttg atgccggttg ggtaa
//
LOCUS       DASTWE010000157         1347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_327276, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000157 DASTWE010000000
VERSION     DASTWE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327276"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFT30_01750"
     CDS             complement(<1..752)
                     /locus_tag="VFT30_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091378.1"
                     /translation="MTVQSTATFLRILRLSLISLSALFIDTSLLHAAEKASTTIFVKD
                     ALTSPNQPATIEAKLISKGLLTSNPLGGEPLELMVGGKVVATAMTGGDGRAFFNYIPK
                     ARGLAPIQVRVGNSPRVASTEGQATLAVLEKRQPILMIELSSLLEEPTPSRVSPRIPP
                     MGIAIELERKPMPEAADELGKLTQFYYRVIYIVTLSAGGDGARVSAEARDWLKAHKFP
                     TGYVMVLPPDSQALGTKIDEFHAAGWKTIKIGV"
     gene            complement(764..1231)
                     /gene="bcp"
                     /locus_tag="VFT30_01755"
     CDS             complement(764..1231)
                     /gene="bcp"
                     /locus_tag="VFT30_01755"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEU5091379.1"
                     /translation="MSKDLAVGDQAPELSIPDQHGKTVTLKSFKGKQIVLYFYPKDDT
                     PGCTKESCDFRDVESQILRAGGAIVGVSLDGKESHQKFIKKFGLPFPLLSDEDAAISK
                     AYGVYKEKNMYGKKYWGIERSTFVIDPEGKLKAIFRKVKVDGHADEVLKALKA"
BASE COUNT          276 a          396 c          347 g          328 t
ORIGIN      
        1 actccgatct tgatggtctt ccatcctgcc gcatggaact cgtcgatctt tgtccctaag
       61 gcttgggagt ccggcggtaa caccatcaca tagcccgttg gaaacttatg cgctttcagc
      121 cagtctctcg cctcagcact cacccgagcc ccatctcctc cggcagacag agtgacgata
      181 tagatgaccc gatagtagaa ctgggtgagc ttaccgagtt catcggctgc ctctggcatc
      241 ggtttccgtt ccaactcgat cgcgatcccc atgggaggaa ttcgaggaga aactcgactc
      301 ggtgtcggtt cttcgagcag cgacgacaac tcgatcataa gaatcggctg tcgcttttcc
      361 aacacggcca gcgtcgcctg cccttctgtc gaagccactc gggggctgtt accgacgcgg
      421 acctggatgg gtgctagacc ccgcgctttc gggatatagt tgaagaacgc ccttccgtcc
      481 ccacccgtca tagccgtggc gacgactttc ccacccacca tcagttcaag cggttctcct
      541 cctagtgggt tgctggtcaa gagccccttc gagatcagtt tggcttcaat agtcgcgggt
      601 tgattcgggg atgtgagcgc atccttaacg aaaattgttg tagaggcttt ctccgcagca
      661 tggagaagag aggtgtcgat gaaaagggca gagagagaaa tgagagaaag ccggaggatg
      721 cgcaggaagg tggccgttga ctgcacagtc atcggccctt cgttcaggcc ttcagcgcct
      781 tgagcacttc gtccgcgtga ccgtcgactt tcaccttccg aaagatcgcc ttcagcttgc
      841 cttcgggatc aatgacgaac gtgctccgct cgatacccca atacttcttg ccgtacatgt
      901 tcttctcttt gtacacaccg taggccttgg agatcgccgc atcttcatcg ctgagcaacg
      961 gaaacggcaa tccgaatttc ttgatgaact tctggtgcga ttctttccca tccaagctca
     1021 ctccgacaat cgcgcctccc gcgcgcagaa tctgcgactc gacgtcgcga aagtcgcaag
     1081 actctttcgt gcacccgggc gtatcgtctt tcgggtagaa gtacagcacg atttgtttgc
     1141 ccttgaaact cttcagagtc accgtctttc cgtgctgatc cgggattgag agttccggtg
     1201 cctgatcccc taccgcaaga tctttgctca taggtccatc cttattcggg aattaataac
     1261 cgccggaggg cataccgcta ccgaacgacg accggcgcgg catattggga cgcagcttag
     1321 ccttgttttt attttcagga ttcccgt
//
LOCUS       DASTWE010000158         1388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_262747, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000158 DASTWE010000000
VERSION     DASTWE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262747"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..665)
                     /locus_tag="VFT30_01760"
     CDS             complement(168..665)
                     /locus_tag="VFT30_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5091380.1"
                     /translation="MAALAKLVDRQEIHDVLMRYCRGIDRCDEELLRSVYHPDATDNH
                     GQFNGKAADFIPWALQSLRRDERTTHFLCNELIEVQGDAAHCESYLFAVHRRKTKDGT
                     ATKDLTFGGRYVDRFERREGVWKIAHRQVIFEWSRIDPVEESYALEQFVTGQRSREDE
                     VYKRS"
     gene            complement(813..>1388)
                     /locus_tag="VFT30_01765"
     CDS             complement(813..>1388)
                     /locus_tag="VFT30_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091381.1"
                     /translation="AHIRLVKAKEPPPPNPNRDNRLRYLVSELIDENESDDTLFDDGE
                     TDWYTNNFWIKTPAGECGAAWGKEITPAGRVANPGWEYSSRPIKKNESATTWLEVSDY
                     DSLSKSDYYDGHSRSPCKHMEFRFEQNTMKIWEEGCGLSLFDDLKEGGYKEVARSMGN
                     GDDGNNDVTVTHKFAVCSISQDPMCSEKCGP"
BASE COUNT          281 a          433 c          343 g          331 t
ORIGIN      
        1 catctcagtg tttgccatgg gatcgccctc cttgagcgta ggactctacc acacgatgcg
       61 gacgtgcgtc agccgtttac ctctgctatc cgccacggtc aaagcgcgac ggtcagccga
      121 cacgttcact ctgtgcctag ccagaatgaa accgtcggga agaacgccta gctgcgtttg
      181 tagacctcat cctcccgaga acgctggccg gtcacgaact gctcgagggc atagctctct
      241 tcaaccggat cgattcggct ccactcgaaa atcacctggc gatgggcgat cttccacacc
      301 ccctctcgcc gttcgaaccg gtccacgtag cgaccgccaa aagtcaaatc cttcgtcgcc
      361 gtcccgtctt tggttttgcg ccgatgcacg gcgaagagat agctctcgca atgggcggca
      421 tcaccctgca cctcaataag ctcgttgcac aggaaatggg ttgtgcgttc gtcgcggcgc
      481 aatgactgca gcgcccacgg gataaaatcc gccgccttac cattgaactg cccgtgattg
      541 tcggtggcat cgggatgata cacgctgcgc aggagttcct cgtcgcagcg atcgatcccg
      601 cggcaatagc gcatcaagac gtcgtgaatc tcctgtctgt ccaccagctt cgccaacgcc
      661 gccaatgttt tctcgtctaa agtcatgtcc cttatctcct gggttgaata gggtcatcca
      721 ctgctatcac ccgagcaggc ccagagaaga gtacgataag gtacccgtaa aaaacaagcg
      781 tgcgcttcgt acgtcaaacc tcccaggcgt tcttacgggc cgcacttctc agagcacata
      841 gggtcttggc taatcgaaca aaccgcgaac ttgtgggtca ccgtcacgtc gttattaccg
      901 tcatcgccat tgcccatact tcgggcgacc tctttgtacc cgccttcctt caggtcatcg
      961 aacaaagaga gcccgcagcc ttcctcccaa atcttcatcg tattttgctc gaaacgaaat
     1021 tccatgtgct tgcagggtga tcgcgaatgg ccgtcgtagt agtcgctctt actgagactg
     1081 tcgtagtcgc tgacttccag ccacgttgtg gcgctttcgt tctttttgat cgggcggctc
     1141 gaatattccc acccgggatt cgctacccgt ccggccgggg taatctcttt tccccacgcc
     1201 gcgccacatt ccccggccgg cgtctttatc caaaagttgt tggtgtacca atcagtctct
     1261 ccgtcatcga acaaagtgtc atcggattca ttttcatcta tcaattccga aacaaggtac
     1321 ctgagccggt tgtcacgatt cggattcggc ggtggcggct cttttgcctt caccaagcgg
     1381 atgtgggc
//
LOCUS       DASTWE010000159         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_36538, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000159 DASTWE010000000
VERSION     DASTWE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36538"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="glyA"
                     /locus_tag="VFT30_01770"
     CDS             <1..922
                     /gene="glyA"
                     /locus_tag="VFT30_01770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5091382.1"
                     /translation="PGDTILGMDLAQGGHLTHGSKVNFSGILFRVFSYGVDRRTETID
                     YDAVQKVAEECRPRMIVVGASAYARVLDFPRFQQIAKSVGAYLLVDIAHIAGLIAAGL
                     HPNPVPYADFVTTTTHKTLRGPRGGVTMCKAEYAKGVDKLVFPGLQGGPLMHVIAAKA
                     VAFKEALSPGFKRYQQQVLTNAKALAQGFIDRGYKIVSGGTDTHLMLLNLTNKGITGK
                     EADAALDTAGIIVNKNAVPYDEKPPAVASGIRLGSPIVSTRGMKEPEMKRIVELVDRV
                     LQHRQEPTVLEEVREQAKALCDGFPIFHSY"
     gene            956..1420
                     /gene="nrdR"
                     /locus_tag="VFT30_01775"
     CDS             956..1420
                     /gene="nrdR"
                     /locus_tag="VFT30_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU5091383.1"
                     /translation="MKCPFCDELEDKVVDSRMAKEGEVIRRRRECLGCKRRYTTYERV
                     EEILPVVVKKDGRRESFDRNKILVGLKKACEKRPISIGTIEAVTDRIEKRIQEMGETE
                     IESRVVGEEVMKELHQLDQVAYVRFASVYREFKDIDQFMDELKTLAQQRRER"
     gene            1528..>1847
                     /locus_tag="VFT30_01780"
     CDS             1528..>1847
                     /locus_tag="VFT30_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5091384.1"
                     /translation="MNKPLPQQRVRRNTPTSGATNVAIIGAGRGGTALMEIFANDPLV
                     QIVGVAELDQKAPGVGLAKQLNIPITQDYRQLLAMERVDLIIDVSGDGEVWQFLQDFH
                     RMGVT"
BASE COUNT          420 a          495 c          543 g          389 t
ORIGIN      
        1 gccgggggac accattttag gaatggacct tgcccaaggg ggtcacctca cgcatgggag
       61 caaagtcaat ttctccggta tcctcttccg tgtcttctcc tatggcgtcg atcgccggac
      121 cgaaaccatc gactatgacg ccgtgcagaa ggttgcagag gaatgccgtc cgcggatgat
      181 cgtggtcggg gccagtgcct atgcccgcgt gctggatttt cctcgcttcc aacagatcgc
      241 caagtcggtc ggtgcctatc tgttagtcga tatcgcgcat attgccggcc tcatcgctgc
      301 ggggctccat ccgaatcccg ttccctatgc cgactttgtc acgacgacga ctcacaagac
      361 gcttcgcggt ccgcgtgggg gcgtcacgat gtgtaaggcc gagtatgcga agggagtcga
      421 caaactggta tttcctgggc tacagggcgg accgttgatg catgtgatcg cggccaaagc
      481 cgtcgccttc aaagaggcct tgtcacctgg atttaagcgt tatcagcagc aagtccttac
      541 caatgcgaag gccttggcac aagggttcat cgatcgtggc tataagatcg tctccggtgg
      601 aaccgataca catctcatgc ttctgaacct cacgaacaag gggatcaccg ggaaagaggc
      661 ggatgccgcg ctggacaccg ccggcattat cgtcaataag aacgccgtgc catacgatga
      721 aaagcccccg gcagtggcca gcgggatccg cctgggttcg cccatcgtct ccacacgcgg
      781 gatgaaagaa ccggagatga agcggatcgt cgagttagtc gatcgtgtcc tccagcatcg
      841 acaagagccg acggtgttgg aagaagttcg cgaacaggcg aaagcattgt gcgacgggtt
      901 tcccatcttt cattcctact agtcagccga tagcgagagg gcagggtcgc cgccggtgaa
      961 atgtcccttc tgcgatgaac ttgaagacaa ggtagtcgat tctcgaatgg ccaaggaagg
     1021 cgaagtcatt cgtcgccgcc gggagtgcct tggttgtaaa cgccgctata ccacctacga
     1081 gcgagtcgag gaaattcttc ccgttgtcgt aaaaaaggac ggtcgccgcg agtcgtttga
     1141 ccgcaataag attctcgtcg gcctgaaaaa agcctgtgaa aaacggccga tcagtatcgg
     1201 caccatcgaa gccgtcaccg accggataga aaaacgaatc caggagatgg gtgagacgga
     1261 gattgaaagt cgggtcgttg gtgaagaagt catgaaggag ctgcatcagt tggaccaggt
     1321 cgcctatgtc cggtttgcat ccgtctaccg ggaatttaag gacatcgacc aatttatgga
     1381 cgagttgaag acgctcgccc agcaacgtcg cgaacgttaa gacgcaactc ctggccccat
     1441 cattctcttt cctggctcgt taatcccatc cgattagacg ttcgcccttg ttgatccgac
     1501 gaccagtgtg caggaggcac agagccgatg aataagccgc tcccccagca gcgtgttcgg
     1561 cgaaacacgc cgacctctgg agcgacgaat gtcgcgatta tcggggccgg tcgagggggg
     1621 actgcgctca tggagatttt tgcgaatgat cctctggtgc agatcgtcgg tgtcgccgaa
     1681 ctagatcaga aagcgccggg agtgggattg gccaaacagc tcaacatccc gattacacag
     1741 gattatcgac agctattggc gatggagcgt gtcgacctga tcatcgatgt gtcgggtgac
     1801 ggagaggtct ggcaattcct ccaggatttt catcggatgg gtgtcac
//
LOCUS       DASTWE010000160         1193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1038341, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000160 DASTWE010000000
VERSION     DASTWE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038341"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFT30_01785"
     CDS             <1..889
                     /locus_tag="VFT30_01785"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEU5091385.1"
                     /translation="QARQLEFGRLVLIAMGYDFKRGRLDLSTHPFTTSFHPTDVRVTT
                     RVHEHELQSCLFSCIHEGGHGLYDQGLDQRYFGTPLGDSVSLGIHESQSRLWENCVGR
                     SRSFWRFFYPILQQTFHQLRGVDIEQFYAAINRVKPSLIRVEADELTYNLHIMLRFEI
                     EQALVENKTQPDDLPAIWNQKMKDYLGIAPSSDAEGVLQDVHWSFGAFGYFPTYTLGN
                     LYSVQFFEQAKLEIPHLEDEIAAGQLMVLRRWLEQKIHRWGRMFTPAHLAQRVTGSTL
                     SPEPFLNYVEQKYGELYQL"
     gene            complement(877..>1193)
                     /locus_tag="VFT30_01790"
     CDS             complement(877..>1193)
                     /locus_tag="VFT30_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091386.1"
                     /translation="PLLLNAIEGVEVNEKVKDILDAVVAAYGTQDSGTITSSVESIQL
                     STPLDPELKIPIRDIVFHTTLLVPHRIEVQAEVAFSPAIASKLNAAMGYDSRPVIDAQ
                     SW"
BASE COUNT          260 a          320 c          325 g          288 t
ORIGIN      
        1 tcaggcccga cagttggagt tcggccgatt ggtcttgatc gcgatggggt atgacttcaa
       61 gcgcggccga ctggacctct caacccatcc cttcaccacg tcatttcatc cgaccgacgt
      121 gcgcgtgacc acccgtgttc atgaacatga attacaatcc tgtcttttca gttgtatcca
      181 tgaaggaggg catggactgt atgaccaggg gttggatcaa cggtatttcg gcacgccgtt
      241 gggcgattcg gtctccctcg gcatccatga aagtcaatct cgtctctggg aaaattgcgt
      301 cggacgctcg cgatcatttt ggcgcttctt ctacccgatt ttgcagcaga cttttcacca
      361 gctgcgcggt gtggatatcg aacaattcta tgcggcgatc aatcgcgtga aaccgtcgtt
      421 gatccgcgtg gaagccgacg agctgaccta caatctccat atcatgctgc ggttcgagat
      481 cgagcaagcg ctcgtggaaa acaagactca gcccgacgac ttgccggcga tctggaatca
      541 gaagatgaag gactacttgg gaattgctcc gtcttccgac gcggaaggag tgctgcagga
      601 tgtccactgg tcattcggtg cgttcggtta ttttcccacg tataccttgg gcaacctcta
      661 ttccgtgcaa ttcttcgagc aggctaagct ggagattccc catttggaag atgagattgc
      721 agccggtcag ttaatggttt tgcgccggtg gctggagcag aaaattcacc gctggggccg
      781 catgttcacg ccggcccatc tcgcgcaacg cgtgaccggt tcaaccctaa gtcctgagcc
      841 ttttctcaac tacgtggaac agaaatacgg cgagctctac caactctgag catcgatgac
      901 aggtcggctg tcatatccca ttgcggcatt gagctttgac gcgatggcag gagaaaacgc
      961 aacttcagcc tgcacctcga tccgatgtgg cacgagcagc gtagtgtgga atacaatatc
     1021 tcgaatggga atcttgagct cagggtctag aggtgtgctc agctgaatcg attcgacgga
     1081 gctcgtaata gtaccgctat cctgagtccc ataggcggcg acgacggcat cgagaatgtc
     1141 tttgaccttc tcgttgacct ccaccccttc gatcgcgttg aggagtaacg gga
//
LOCUS       DASTWE010000161         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_101277, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000161 DASTWE010000000
VERSION     DASTWE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101277"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VFT30_01795"
     CDS             <1..102
                     /locus_tag="VFT30_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091387.1"
                     /translation="ELISPIAMDQGLRCAVREGGKTVGSGVVTEILA"
     gene            185..334
                     /gene="rpmG"
                     /locus_tag="VFT30_01800"
     CDS             185..334
                     /gene="rpmG"
                     /locus_tag="VFT30_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5091388.1"
                     /translation="MREIIDMACTLCKQRNYSSMKNKKNDPDRLERNKFCKFCRKHTP
                     HKEVK"
     gene            419..493
                     /locus_tag="VFT30_01805"
     tRNA            419..493
                     /locus_tag="VFT30_01805"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Trp,seq:cca)
     gene            738..932
                     /gene="secE"
                     /locus_tag="VFT30_01810"
     CDS             738..932
                     /gene="secE"
                     /locus_tag="VFT30_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU5091389.1"
                     /translation="MFKRMTESIRLFVTDVRAELKKVSFPSRAETIGSTTVVIVFCIL
                     MSLYLSVIDSFLSWLVGKFI"
     gene            994..1530
                     /gene="nusG"
                     /locus_tag="VFT30_01815"
     CDS             994..1530
                     /gene="nusG"
                     /locus_tag="VFT30_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU5091390.1"
                     /translation="MTKNWYVIHTYAGFEGRVKTSLMERANQMGLVEKVGQVLVPTED
                     VIEIKDGKRRTSRRKFFPGYVLVELESPLGDETLQMIKETPKVTGFVGGGTVPTPLTN
                     EEVESLLKQVDAGQAEPREQVKFIKSDNVRIIDGPFLGFNGVVDEVDQDHSRLKVMVS
                     IFGRSTPVELGFLQVERI"
     gene            1611..>1836
                     /locus_tag="VFT30_01820"
     CDS             1611..>1836
                     /locus_tag="VFT30_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5091391.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKDGDSIIPVIITVYKDRTFSFIMKTPPA"
BASE COUNT          438 a          417 c          567 g          414 t
ORIGIN      
        1 gagttgatca gtccgatcgc gatggatcag gggttgcggt gtgccgtgcg cgagggcggc
       61 aagacggtgg gctccggcgt cgtcaccgaa atcctggcat aggtaagggt tgatcgtcat
      121 gcgtctaccg tcggtcgaaa aaggaactct tccggattgc gattgacgaa ttacggagca
      181 gaaaatgcgc gaaatcatcg acatggcttg tacgctctgc aaacagcgga attactcgtc
      241 catgaagaac aagaagaacg atccggatcg gttggaacga aacaagttct gtaagttctg
      301 ccgaaagcac acgcctcata aagaggtgaa ataagatgag gcgcaagggg tgacgcgttt
      361 ggggtgagga acctcaccga ttggctgacg ttgcacgcct cgcgtttcac gttcgagtag
      421 gggcatggtg ttaatggcag cacatcggtc tccaaaaccg agagtctggg ttcaaatcct
      481 agtgcccctg ccaaacagag cttgctcgac ccggagcgct tcacatgatg cgcgacggga
      541 taagcacctc acgttggggc tggattttgt gtagatcgct cgctcgtgcc aggagccgta
      601 agatggttct cggcgaccgg atgaagcacc ttgcgggatg tgaccgtttg aaaatagctt
      661 gctcgacccg gagcgcgtgc aatgttatgc gacgggatca tccccgcaag tgttagggtg
      721 acgaaacgga gttagtcatg tttaagcgga tgacagagtc gattcggctg tttgtgacgg
      781 acgtgcgggc agagctgaaa aaggtttcat ttccgagtcg tgcggaaacg attggctcaa
      841 cgacggtggt gatcgtgttc tgcatcttga tgtcgctgta cttgtccgtc attgactcat
      901 tcctttcatg gttggtcggc aaattcatct aagcctgccg agaagtggta aggggaattc
      961 agcggtaatt catcgaagat ctgagggtgg tgcatgacaa agaactggta cgtcatccat
     1021 acgtacgcgg gttttgaggg gcgagtgaag accagcctca tggagcgtgc aaatcaaatg
     1081 gggcttgttg agaaggtcgg gcaggtgctc gttccgacag aggacgtgat tgaaattaag
     1141 gatgggaagc gacggacttc tcgacgaaag ttcttcccag gctacgttct cgtggagttg
     1201 gagtctccgt tgggggacga gactttgcag atgatcaagg agacgccgaa agttacggga
     1261 tttgtcgggg gaggcacagt tccgacccca ctgactaatg aggaagtgga gtccctgctc
     1321 aaacaggtcg atgcgggtca ggccgagcct cgagagcagg tcaaatttat caagagcgac
     1381 aacgttcgca tcatcgatgg tcccttcctc gggttcaacg gcgtggtcga cgaagtcgat
     1441 caagaccata gtcggttgaa ggtgatggtg agcatcttcg gccgatcgac cccggttgaa
     1501 ttgggattct tacaggttga acggatttaa gtcgatcggt cggcgtcggc cttcagtcac
     1561 gattgagggc tgaaagttga ttgtcgatcg cgaaggagaa agagaacgaa atggcgaaag
     1621 aagtttcggc gcagatcaag ttgcaaattc cggccggcaa agccaatccg gctcctcctg
     1681 tcggcccctc cttgggtcag catggcgtca atattatgga gttttgcaag cagttcaatg
     1741 ccaagaccca gaaggatggg gatagcatca tccctgtcat cattacggtc tacaaggacc
     1801 gtactttcag cttcatcatg aagacgcccc cggctt
//
LOCUS       DASTWE010000162         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_521358, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000162 DASTWE010000000
VERSION     DASTWE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521358"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..978
                     /locus_tag="VFT30_01825"
     CDS             265..978
                     /locus_tag="VFT30_01825"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU5091392.1"
                     /translation="MARLGVNIDHVATLRQARGGSDPDPLAAAVLVELAGADGLVVHL
                     REDRRHIQDRDLQLLREIVRTKLDLEMAADGEMAKIALNIKPDLVTLVPEKRQELTTE
                     GGLDIVGQRERIQGIVTLLHNGGIPVSVFVEPDLNQIKAAHKIAADFVELHTGRYANA
                     TRSKEADAEFEAITHAAKLAYKLGIGVNAGHGLNYRNIKRLTHIPEIVEYNIGHSIVA
                     RAVLVGLVQAVKEMKALLD"
     gene            1030..2610
                     /locus_tag="VFT30_01830"
     CDS             1030..2610
                     /locus_tag="VFT30_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5091393.1"
                     /translation="MTKIITAVQMQELDRRTISEAHIPATTLMERAGSGVVSCLEQRL
                     GPVRGKTVTVICGKGNNGGDGFVAARLLRRRHANVRVLAMAPSSELSRDAATMYKQFV
                     RGRGKSSVYLYTSKAQAQVLLQDSDILVDALLGTGLSSAVTGRYAEVIDCINEAGCPV
                     VAVDLPSGLHADTGTILGRAVRASFTVTFGLPKLGLYQNNGIDLAGQVVIEDIGIPPA
                     YINAVESRTTLITEHMVRAYLPKRQLSSHKGTFGHVGIIAGSVGKTGAAALAARAALR
                     IGAGLVTVAIPTSVNDTLEAKLLEAMTLPMPETKARTFARTALDRLVAFMAARTAIAI
                     GPGLSTHPETVELVQALTKQLDRPAVLDADALNALTGRTALLASCKTPPIITPHPGEM
                     ARLEADATPQTVNNDRIGTASRFARERGLFVVLKGARTVVARPDGAVAICPTGNPGMA
                     TAGTGDVLTGMMVGLLAQGMPSWEAACAATYLHGAAGDLAAARMGQAGMISGDVIEEI
                     PYALRQIHQISSDKINKI"
BASE COUNT          603 a          723 c          730 g          593 t
ORIGIN      
        1 attccccaaa ctaactcatc gcgataaatg agcgaaggga tgtggaaagc gcatatttta
       61 atggagggcc ttggtcgagt caatttaccc cattcttatg gagttctgag ggtgccaaga
      121 ccagaaggct ttcatcagtt cttcgcctat cgacgaaccg gttcttgccc cctctcgttc
      181 ctttctgcta ctaatatcgt ttgattactg acctagcttg gtctcagtcg cttaatatca
      241 agttgattgg ataaaggaga gtggatggcc cggcttgggg taaatatcga ccacgtggcg
      301 acattgcggc aggcacgtgg cggatccgat ccggatcctt tggctgctgc tgtacttgtc
      361 gagttggcgg gagcggatgg tttggtcgtt cacctccggg aagaccggcg tcatatccaa
      421 gatcgagatc ttcagctcct tcgtgaaata gtccgcacaa agctcgatct tgaaatggcg
      481 gccgacggcg aaatggcgaa aatcgccctc aacatcaagc ccgaccttgt cacattagtc
      541 ccggaaaagc gacaagaact caccacagaa gggggccttg atattgtagg acagcgcgaa
      601 cgaattcagg gcattgtgac tctgttacat aacgggggga ttcccgtcag tgtctttgtc
      661 gaacctgatc tgaatcagat taaggccgcc cacaaaattg cagctgattt cgtggagttg
      721 cataccggtc gttacgcgaa tgccacacgc tccaaagaag ccgatgcgga atttgaagcc
      781 attacccacg ctgccaagct ggcttacaaa cttgggattg gggtcaatgc cgggcatgga
      841 ctgaattacc gcaatatcaa acgcctgacg catattcccg aaatcgtcga gtacaacatt
      901 gggcacagta tcgtcgcacg ggcagtcctg gtgggtctcg tacaagccgt gaaagagatg
      961 aaagccttgc tggattgagt tgatgtcctc gattcgatag gtccgcctat tcaccgcatt
     1021 cggttcacca tgaccaaaat catcaccgct gtgcagatgc aggagctcga ccgtcgaacg
     1081 atctcagagg ctcatatccc agcgacgacg ttgatggaac gtgccgggtc cggcgtcgta
     1141 tcttgccttg agcaacggtt gggacccgta cggggcaaga ccgtcactgt tatttgcgga
     1201 aaaggcaaca atgggggaga tggattcgtc gctgctcgtc tactccgtcg gcgccatgcc
     1261 aacgtgcgcg tactcgccat ggcacccagt tccgaactga gccgcgatgc ggcaacgatg
     1321 tataagcaat tcgttcgggg cagagggaaa tcttccgtct acctctatac gtccaaggct
     1381 caggcccaag ttcttttgca ggacagcgac atcctcgtcg acgcactcct cggtacagga
     1441 ctctcctccg ctgtaacggg gcgctatgcc gaggttattg actgtatcaa cgaagccggt
     1501 tgccccgtgg tggccgttga tctgccatcg ggcctccatg ctgataccgg aactatcctg
     1561 ggccgagccg ttcgtgcctc cttcaccgtg acctttggtc ttccgaagtt aggcctctat
     1621 caaaacaatg gtatcgacct cgccggacag gtggtcatcg aggacatcgg aattccaccg
     1681 gcttacatta acgcggttga gagccgaacg accttgatta cagagcacat ggtacgggca
     1741 tacctgccga aacggcagct atcgagtcac aaaggtacat ttggtcatgt gggcattatt
     1801 gccgggtccg ttggaaaaac tggcgccgcc gccttggcgg cacgagcggc cctgcgtatc
     1861 ggcgcgggcc tcgtcaccgt cgcaatccct acaagcgtca acgatacatt ggaagcaaag
     1921 ctgttagagg caatgacact tccgatgcct gaaaccaaag cgcgcacctt tgcacggacc
     1981 gcattggatc ggctcgtagc cttcatggcg gctagaaccg caattgccat cggtccagga
     2041 ttatccactc atcctgaaac ggttgaactc gtccaggcct tgacgaaaca gcttgaccga
     2101 ccggctgttt tggatgcaga tgccctgaac gcgttgaccg gacgaactgc tcttttggcg
     2161 tcttgtaaaa caccgccgat tatcacgccc catcccggag agatggcgcg actggaggct
     2221 gatgcaacgc ctcagaccgt caataatgat cggatcggta ctgcctcccg cttcgcgcga
     2281 gaacgcggcc tgttcgtcgt tttgaaaggt gccaggaccg tcgttgccag accggatgga
     2341 gccgttgcta tttgccctac cggcaatcca gggatggcga cggcaggaac tggcgatgta
     2401 ttaaccggta tgatggtggg actcttagcc caaggcatgc cctcctggga ggctgcctgt
     2461 gccgcaacgt acctgcatgg agccgccggc gatttggctg cagccagaat ggggcaggca
     2521 gggatgatct cgggagacgt gatcgaggag attccctatg ccctcaggca gatacatcag
     2581 atttcatcag ataaaatcaa caagatatag accgatcccc tgtgaggacg ttcccatggc
     2641 tacatctat
//
LOCUS       DASTWE010000163         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_973917, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000163 DASTWE010000000
VERSION     DASTWE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973917"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..>1144)
                     /locus_tag="VFT30_01835"
     CDS             complement(219..>1144)
                     /locus_tag="VFT30_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEU5091394.1"
                     /translation="QTALRNVIEPIFEHEFDSCSFGFRPNLGCQNALEEIERQLAAGS
                     IHVVDVDLRKYFDTIPKQALMDEVAKKIADGRVLDLIEAYLQQGVLEEMRLWTPEKGT
                     PQGAVISPLLANVYLHPVDVAMRQAGFTLVRYADDMVVLCPTREEAEAALVRLNELIE
                     ARGLQLHPDKTRIAHLMVRPGFQFLGYVFYDKYRDPRPSSKDKLRATIRRKAKLTNGN
                     SLKDIISDLNASLHGWFNYFQFCSANSWAWAEIDKWVRFRLRAILDKRQKSGRKRRRG
                     RGYAHYRWPNAYFTEHGLFSLVSAVKLVRQP"
BASE COUNT          218 a          329 c          337 g          260 t
ORIGIN      
        1 ggagcgtggc ccacggcgtt cgccaatgtc gcgtttacgc tggtgctctg tttcgccgca
       61 ggactcgcgg gtgcggcgat cgggcgcgtg atgcagtaac gggtcacgac tgacgggcag
      121 gatgaacgat agaggtaggg agaggcctta ggcctccccc tccctccgaa ccggacgggc
      181 ggatctcccg catccggctc tccagttggt gatcttgttc agggctgtcg gacgagctta
      241 acggcgctca cgagactgaa gagaccgtgc tcggtgaagt atgcgtttgg ccagcgataa
      301 tgagcgtagc ctcgcccacg gcgtcgtttt cttccgctct tttggcgttt gtcgagaatg
      361 gcccggagac ggaagcgaac ccacttgtca atctccgccc atgcccaact gttcgccgaa
      421 cagaactgga agtagttgaa ccagccgtga agagaagcat tcagatccga gatgatgtcc
      481 ttgagtgagt tgccgttggt cagcttcgct ttccggcgga tcgtggcgcg gagtttgtcc
      541 ttgctcgaag gccgtggatc gcggtacttg tcatagaaca catatcccag gaactgaaag
      601 ccaggtctta ccatcaggtg cgcaatgcgc gtcttgtccg ggtggagttg cagtcctcgc
      661 gcttcaatca gttcgttgag cctgaccagc gccgcttcgg cctcttccct tgtggggcag
      721 agcaccacca tgtcatcggc atatcgaaca agcgtgaacc ctgcttgccg catggccaca
      781 tcgaccgggt ggagatacac atttgccaga agcggactga tgaccgcacc ttgcggcgtc
      841 cccttctccg gagtccagag tctcatctcc tcgagaaccc cctgttgtag atacgcttcg
      901 atcagatcga gcactctgcc atcagcgatc ttcttcgcga cttcgtccat cagcgcttgc
      961 ttggggatcg tgtcgaagta ctttcgcaga tcgacgtcga ccacatggat cgatccggct
     1021 gcgagctgtc gttcgatttc ttccagtgcg ttttggcatc caaggttggg acggaagcca
     1081 aaactgcagg agtcgaactc atgctcgaag atcggctcga tcacgttccg caaggctgtc
     1141 tgaa
//
LOCUS       DASTWE010000164         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_295160, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000164 DASTWE010000000
VERSION     DASTWE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295160"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..2103
                     /gene="dxs"
                     /locus_tag="VFT30_01840"
     CDS             160..2103
                     /gene="dxs"
                     /locus_tag="VFT30_01840"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU5091395.1"
                     /translation="MSILKTIHSPADLKRLSPNKFPALCREIREQIIGAVSNVGGHLA
                     SNLGVVELTVALQYLLDTPTDKIVWDTSNQSYTHKLLTGRREQFHTLRQYGGLSGFCK
                     REESEYDTFNAGHAGTGVSAAFGMVEARDQLKQKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGLGKDFLVILNDNQMSISKNVGAISAYLSRTITGEFYGKMREETGQLLGKIPHIGSD
                     IQKLARRAEELAKGVILPGLLFEELGFQYSGPIDGHNFEHLLPTIENVLKMKGPVLLH
                     VITKKGLGYEPAMKNPVWFHACPPFVRSTGAPAKKAARPSYTAIAMDALVKLAREDKR
                     VVAITAAMCEGTGLTAFEKEFPDRLYDVGIAEQHAVTFAAGLAAQGMKPVVAMYATFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDYAYLRHVPNMVVMAPKDE
                     NELQHMVKTCLEFNGPISVRYPRGVSLGVKMDPVPQALPVGKGELLKDGTDVAIIAIG
                     VSVWQAVEAAERLGKEGVSTAVVNGRFVKPLDHELIVDVAKRVRYVVTVEEGCKIGGF
                     GSAVLETLSEAGVTEVKTKVLGLPDWYIEQGPQDLLRERYGLTAEGIYQSVKELIGKA
                     PVVNSQFTGAALAGHPHGDEQGS"
     gene            2137..3321
                     /gene="ispG"
                     /locus_tag="VFT30_01845"
                     /gene_synonym="gcpE"
     CDS             2137..3321
                     /gene="ispG"
                     /locus_tag="VFT30_01845"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5091396.1"
                     /translation="MHIARRKTRQITVGKVKVGGDAPVSVQSMCSTDTRDVAATVAQI
                     HQLEAAGCEIIRVAVPDEEAAQVLPQIKAAMTVPLIADIHFDHRLALKAAQIVDCVRI
                     NPGNIGAWWKVEEVIKAVNERGIPLRVGVNGGSLERPLLEKYGWPSPEALAESALNAV
                     HALEDVGFTNMKVSLKASDVHHAIDAYWLFAHQSDYPLHIGITEAGTAMTGAVKSSIG
                     LGYLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLVRKV
                     PMEELMDTLIQEVELLAKEKEAESNGDATASVPSNGWESLEPQSDQSSTLGREIPVLP
                     HR"
BASE COUNT          792 a          841 c         1048 g          731 t
ORIGIN      
        1 atcacgaggc catgagagag ggatccctcc aggtcatgtt tcataagtgc ttgacatcat
       61 atggttcttg tgtgagactc caccaaattt ggcacccgaa ctaagggtgc tcattgtgta
      121 agccactttg ctgacggggg agaacgaagg agaccatcta tgtccatact caagacgatc
      181 catagtcctg ccgatctgaa gcgattgtct cccaacaaat ttccggcatt atgccgggag
      241 attcgtgaac agattatcgg ggccgtttcg aatgtgggcg gacacctggc ctcgaattta
      301 ggtgtcgtcg agctcacagt cgccttgcaa tatctcctgg atacaccgac cgacaaaatc
      361 gtttgggata ctagcaatca atcatacacg cacaaactcc tcaccggtcg gcgtgagcag
      421 ttccacacgc ttcgccagta cggcggattg agtggatttt gtaagcggga agagagtgag
      481 tacgatacgt tcaatgccgg ccatgcgggg actggtgtgt cggccgcctt cggcatggtc
      541 gaagccagag atcagttgaa gcaaaagcat aaggtcgtct gtgtcgtcgg tgacggcgcc
      601 atgacggcgg ggatgacgct ggaaggactg catcatgccg ggggactcgg aaaagatttt
      661 ctcgtaattt tgaacgacaa ccaaatgtcg atctcgaaaa acgtcggggc tatttccgct
      721 tatctgagtc ggaccatcac gggtgagttc tatgggaaaa tgcgtgaaga gactggtcag
      781 ctattgggta agattcctca catcggctcc gacatacaga aactcgcccg ccgggccgaa
      841 gagctcgcca aaggcgtgat tctgccggga ttgctctttg aagaactggg atttcaatac
      901 agtgggccca tcgacggtca caactttgag catctccttc cgacgattga gaatgtgctg
      961 aagatgaagg ggccggtcct gcttcatgtc atcacgaaga aggggctcgg ttacgaaccg
     1021 gccatgaaga atccggtatg gttccatgcg tgccctccgt tcgttcggtc gaccggcgca
     1081 ccggccaaga aagccgctcg cccgtcctat acggcgatcg ccatggatgc gctcgtcaag
     1141 ttggctcgtg aggacaagcg ggtcgtggcc atcacggctg caatgtgcga aggtacgggg
     1201 ctaacggcat tcgaaaaaga atttccggat cgtctctacg atgtcggcat cgccgagcag
     1261 catgcggtga catttgcggc gggactcgcc gcgcagggca tgaaaccggt cgtggcgatg
     1321 tatgcgactt tcctccagcg cgcgtatgat caagtcgtgc atgatgtcgc cacccagaat
     1381 cttccggtga ccttctgcat cgatcgagga ggactcgtgg cggaggacgg gacgacgcat
     1441 catggggcct tcgactatgc ctatttgcgg cacgttccta atatggtcgt catggctccc
     1501 aaagatgaaa atgaactgca gcatatggtg aaaacctgcc tggaattcaa cgggccgatt
     1561 tcggtgcgat atccgcgtgg ggtgagtctc ggtgtgaaga tggatccggt tccccaagcc
     1621 ttgccggtag gaaaaggtga gcttctcaaa gatggaacgg atgtggccat cattgcgatc
     1681 ggtgtctcgg tatggcaggc cgtcgaagca gccgaacggc ttggtaagga aggggtttca
     1741 acagcggtcg tgaacggacg attcgtcaag ccactcgatc acgaactgat tgtcgatgtg
     1801 gcgaagcggg tgcgctatgt tgtgacggtg gaggaaggct gcaagatcgg cgggtttggt
     1861 tctgccgtgc tggaaaccct ttcggaagca ggggtgacag aggtgaagac gaaggtccta
     1921 ggcttacccg attggtacat cgaacagggg ccccaggatc tgttgcggga acggtatggt
     1981 ttgacggctg aagggatcta tcaaagtgtg aaggaattga tcggcaaagc accagtcgta
     2041 aactcgcagt tcacaggtgc ggcactagcc ggccatccac atggagatga acaagggagc
     2101 tgatcggaaa gagtaagggt aagagagtta catcagatgc atatagccag acgaaaaact
     2161 cgacagatta cggtcggcaa ggtgaaggtt gggggcgatg ctccggtctc cgtgcaatcc
     2221 atgtgttcga cggacacgcg cgatgtggcc gcgacggtcg cgcagattca ccagcttgaa
     2281 gccgccggct gcgaaatcat ccgcgtggcg gttccggacg aggaggcggc ccaggtcctc
     2341 ccgcaaatca aagcggcgat gacggtcccg ctgattgccg atattcactt cgatcatcgg
     2401 cttgccttga aggccgcgca gatcgtcgat tgtgtccgca tcaatcccgg taatatcggt
     2461 gcttggtgga aagtggaaga agtcattaag gctgtgaatg agcgagggat tccccttcga
     2521 gtcggcgtca atggagggtc gctcgagcga ccgttgttgg agaagtacgg atggccttcc
     2581 ccggaggcgc tggcagagtc ggcactcaat gccgtacatg cgcttgaaga cgtgggcttt
     2641 accaacatga aggtgtcgct caaagcttcg gacgtgcatc atgcgattga tgcctattgg
     2701 ctctttgccc atcagtccga ttatccgctt cacattggga ttacagaagc agggacggcc
     2761 atgactgggg cggtaaagtc ttccatcggc cttgggtact tgctttcgca ggggatcgga
     2821 gacacgttgc gggtatcgct ggccgctgac ccggtggagg aagtcaaagt cgggtttgaa
     2881 atcctcaagt cactcgaatt acgccatcgg ggcattaatg tcatcgcgtg cccgacatgt
     2941 gggcgcgtcg agatcgacgt cgtccggatg gcgaacgagt tggaaaagaa actcggccac
     3001 atcaagaccc cattgaacgt gtcggtgctc gggtgtgttg tgaacgggat cggggaagga
     3061 aaagaagccg acatcggaat cgccggcggc gaggggaagg gtattttatt caagaagggc
     3121 aagctcgtcc gaaaggttcc gatggaagaa ttgatggaca ccctgattca ggaagtggaa
     3181 ttgttggcca aggaaaaaga agcggaaagc aacggcgacg cgactgcatc tgttccttca
     3241 aacgggtggg aatcgttgga accccagtcg gatcagtctt cgaccctcgg cagagaaatc
     3301 ccggtcttgc ctcaccggta ggtgtattgt cgacaacgac tctcccactg cttcactgag
     3361 ctgcacgccc ctctcaccaa aaaacgattg acgcactttc acagcgtcat gt
//
LOCUS       DASTWE010000165         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1038485, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000165 DASTWE010000000
VERSION     DASTWE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038485"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFT30_01850"
     CDS             complement(<1..354)
                     /locus_tag="VFT30_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5091397.1"
                     /translation="MRIPSWEIGRALGIPIRIHASWFLVFLLVTWTLSTGYLPESLPG
                     LTPERYWAMGGIAAVLLFLSVLLHELGHSYVALYYRIPIEKITLFIFGGVAHMRKEAP
                     TPRAELLIALAGPAVS"
     gene            424..1143
                     /locus_tag="VFT30_01855"
     CDS             424..1143
                     /locus_tag="VFT30_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5091398.1"
                     /translation="MRRPVLLYLLLGALSAATVGPFASVDGVAQVIKSGPPTCQGVAL
                     TFDLCPVRKGSGYDLSLMDYLINHRIPATFFMSGKWIAKHEPEVDHLLGLEFFEVGTH
                     GEVHAHLPMHDADEQRTEILGPIKTLRDYYAHDATLFRPPYGEYNDVTVAVVNMLGLR
                     FIQWNIESGDPDPTLSAEQILTRVATRAKPGSIIVFHANGKGKHTRQVIEQLTSDILP
                     RKGLQPMTVSELLTCTPGAHD"
BASE COUNT          300 a          335 c          315 g          245 t
ORIGIN      
        1 acttaccgcc ggcccggcca acgcaatgag gagttcggca cgaggtgtcg gggcttcctt
       61 ccgcatgtga gccacgccgc cgaaaataaa caacgtgatt ttttcaatgg ggatacgata
      121 gtacaaagcg acgtaggaat ggccgagctc atgcaacagc acggagagga ataaaagcac
      181 cgctgcgatg ccacccattg cccaataacg ttcgggagtg agtccgggca aactttccgg
      241 aagatatcca gtcgagagag tccatgtgac caggagaaag accagaaacc atgacgcatg
      301 gatacggatc ggaattccta acgcccggcc gatttcccag gaaggtatac gcataggcca
      361 caccgtaaca gccgaatacg taaagggtca attcttctcc accggttcag gtatactggc
      421 cctatgcggc gacccgttct gctatacctg ctgctcggcg ctctgtcagc agcaactgtc
      481 gggccattcg cttcagtaga cggtgttgcc caagttatca agtccggccc gccaacgtgc
      541 cagggcgtgg cactgacgtt cgatctttgt cccgtacgga aggggtcagg ctacgatctg
      601 tctttgatgg actatctgat caatcatcgg atccccgcca cgttcttcat gtccggcaag
      661 tggatagcaa aacatgaacc ggaagtggac catctattag gcctggaatt ttttgaagtg
      721 ggcacgcacg gtgaggtaca tgcccacctt cccatgcacg atgccgacga gcagcgcacg
      781 gagatccttg gccccatcaa aacactacga gattactatg cccatgatgc gacgttgttt
      841 cgaccaccgt atggagaata caacgacgta accgtcgccg tagtgaacat gctcggcttg
      901 cgttttatcc aatggaatat cgaatcgggc gatccggacc ccacactgtc tgccgagcag
      961 attctcacca gagtcgccac gcgtgccaag ccagggagca tcatcgtctt ccatgcgaat
     1021 ggaaaaggaa agcacactcg gcaggtgatt gaacaactta catcggacat tctgcctcga
     1081 aaaggactcc aacccatgac cgtcagcgag ctgttgacct gtactcctgg agcccatgac
     1141 tgactcggtt attaggaaca tggatccgga cgatcgagag tccgtcgtcc aactg
//
LOCUS       DASTWE010000166         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1426293, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000166 DASTWE010000000
VERSION     DASTWE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426293"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1038)
                     /locus_tag="VFT30_01860"
     CDS             complement(46..1038)
                     /locus_tag="VFT30_01860"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5091399.1"
                     /translation="MIRVRVIGTGSYLPSRVVPNEEIASRLRLSPARIQRLTGIRTRH
                     WAGDHEASSDMAIVAGRRALDAADCAASSIDAIILSTTSPDMAFPSTACLVQRGLGCR
                     QVGAFDVSASCSGFLYGLSMAQAMIQSGQTKTCLVVASEVKSRSLDLQDEATALLFGD
                     GAGAVILQGEENRSPEWRGILGIKLYADGSHHGLIRVPAGGSRTPLSSDRLRNQEHTL
                     RMQGASLFRIAVRRIEQAVLEIMKEFGLCPADLKQVVLHQANGRILSHVADRLGIDQN
                     RLASVIERYGNTSSASLPIALDDAVRSGDISSGDLVLLGSFGGGLTWATGLVRW"
     gene            1280..>1938
                     /gene="bamD"
                     /locus_tag="VFT30_01865"
     CDS             1280..>1938
                     /gene="bamD"
                     /locus_tag="VFT30_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU5091400.1"
                     /translation="MVSCAGDFSPSDVRSGIKKALSGTDEQIFLGDTVENHYHPNVIM
                     KRGEAYFEKEEYAEALVEYKHFMELHHNHILAPYAAFRIGEIHFKMAKTIDRDPEPMQ
                     KAIAAFEQMRKEFSGSRYDSQAQQKLEECHDWIAQMHLFVGQFYYRRGSYLAATHRFQ
                     QILKNYPDKPIAAEALYFLAKAHHEMGADDWARENLIVLAEKYPNSTASGAGRSLLEK
                     IG"
BASE COUNT          500 a          528 c          487 g          423 t
ORIGIN      
        1 tcgatgatga gcgaccaaac gaatcgctac tctcgttcca cgtctttacc atcgtacgag
       61 ccccgttgcc caagtcagcc cgccaccgaa actgccgagc agaaccagat caccagacga
      121 gatgtcgccg ctgcgaaccg cgtcatcgag cgcgatggga agggacgctg aggacgtatt
      181 gccataacgt tcgatcaccg atgccaatcg gttctggtcg atacccaggc gatcggcaac
      241 gtgagacaag attcgaccgt tcgcctggtg gaggacaacc tgtttgagat ctgctggaca
      301 aaggccgaat tctttcatga tttccagcac ggcctgttca atccgacgaa cggctatccg
      361 aaagagcgat gcgccctgca tacgaagcgt atgctcctga ttgcgcaacc tgtcggatga
      421 caagggtgtc cgtgaccctc cagccgggac tcgaataagt ccatggtgtg acccatcggc
      481 atacaatttg atcccaagaa ttcctcgcca ctcagggctt cggttctctt cgccctgtag
      541 tatgacggct ccagccccgt cgccgaacaa cagcgccgtc gcttcatcct gaaggtcgag
      601 tgatcgagac ttcacctccg aggccacaac aaggcaggtc ttggtctgtc cgctctgaat
      661 catggcctgt gccatggaga gcccatagag aaatcctgag caggaagcgg acacatcaaa
      721 tgcccccact tgtctacatc ccaatcctcg ttggaccaag caagctgttg aaggaaaggc
      781 catgtcggga gaggtcgtag ataaaatgat ggcatcgata gaagaagccg cacaatccgc
      841 ggcatcaagt gcccggcgcc ccgccacaat ggccatatcg gaagacgcct cgtgatcccc
      901 ggcccaatga cgggttcgaa taccggtcag ccgttggatt cgcgccggtg atagtctcaa
      961 tctggaagct atctcttcat tgggtacgac ccgagaaggg agataactgc ctgttccaat
     1021 gacccttact cgaatcatgc ccataatcac atctacacga acaaaattgt gcgtactcca
     1081 acgtgggccg ggattatagg gagcatgagg gggcaggtca accaaagtgc actcaaaccg
     1141 ccagtatcat tgctttttgc ccacatcctt gctatctcta ttccccatgc atcctcaatc
     1201 catccgaatc agttccagtc cgttatcaaa gtcatcaggc ctatgcctgg cgatgtggct
     1261 cggcattggc tctctgttca tggtttcatg cgctggtgat ttctctccta gcgatgtgcg
     1321 aagcggaatc aagaaagcgc ttagtggcac cgacgagcaa atttttctcg gagatacggt
     1381 tgaaaatcat taccacccta atgtcatcat gaagcggggg gaagcctatt ttgagaaaga
     1441 agagtatgcg gaggcattag ttgagtataa acacttcatg gaactccatc ataatcacat
     1501 cctggccccg tatgcggcat ttaggatcgg cgaaattcat ttcaagatgg cgaaaaccat
     1561 cgatcgtgat cctgaaccga tgcaaaaggc cattgccgca tttgagcaaa tgagaaaaga
     1621 attttccggc agccgctatg atagccaagc tcagcaaaaa ctcgaggaat gccacgattg
     1681 gatcgctcag atgcatctct tcgtgggaca attctattac cgacgaggct cctacctcgc
     1741 agctacacat cggttccagc aaatcttaaa aaattatcct gataagccga ttgccgcaga
     1801 agccttgtac ttccttgcta aggctcacca tgaaatggga gccgacgatt gggcaaggga
     1861 gaatctgatc gtattggcgg aaaaatatcc gaacagtaca gcctcagggg ctggaaggag
     1921 cttactggag aaaatcgg
//
LOCUS       DASTWE010000167         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_166029, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000167 DASTWE010000000
VERSION     DASTWE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166029"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFT30_01870"
     CDS             <1..227
                     /locus_tag="VFT30_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091401.1"
                     /translation="AALEWGLQNIRVNLVLPGWQKTDLTKGLFPEGQDWPDHALHRPA
                     ALDEVVGTILHVAQLKDVSGQIWNCDSRHL"
     gene            339..>1006
                     /gene="bioD"
                     /locus_tag="VFT30_01875"
     CDS             339..>1006
                     /gene="bioD"
                     /locus_tag="VFT30_01875"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEU5091402.1"
                     /translation="MNRGIFITGTDTGVGKTLVTAALALHLKKGGLTVGVMKPIETGV
                     SAGREARSDAARLRSIIESEETPGAICPYSFELPVAPLTAAQMDGRSINLGTISKIYR
                     LLSGRYECMVVEGVGGVQVPITPSDNVLSLIKHLRLPVVVVGRSGLGGINHALLTIET
                     LRRQNISMIALVLNRTHPVRSALARVQERSTLEILRKQAGIPVIGPLPYESGLQSRFR
                     PAII"
BASE COUNT          234 a          278 c          276 g          218 t
ORIGIN      
        1 ccgccgcgct agaatggggg ctgcagaata ttcgcgtgaa tctcgtgttg cccggctggc
       61 agaagactga cctgaccaag gggctctttc cagaaggaca agactggcct gatcacgcac
      121 ttcatcgtcc agccgctctc gacgaagtcg tcggtacgat cttgcatgtt gcccagctca
      181 aggacgtatc agggcagata tggaattgtg acagcagaca tctctaacaa gagcgtatgg
      241 ctggtggctt atagcctata gccagacgca tactgaagac gcgtgatggg tcgatccgaa
      301 acgatacgcc atatgctata cgccataagc tctttcatat gaaccgcggc atcttcataa
      361 cgggtaccga tactggcgtt gggaagacat tggtcacggc ggcactcgct cttcatctga
      421 agaagggcgg gctgacggtt ggcgtgatga aaccgattga gacaggagtc tcggcgggaa
      481 gagaagctcg atcggatgca gcacgactac gatccatcat agaaagtgaa gagacaccag
      541 gagctatctg tccctattcg tttgagttgc cagtcgctcc gcttaccgct gcgcagatgg
      601 atgggcgcag catcaatctc ggcactatca gcaagatcta tcgtctctta tccggtcgat
      661 atgagtgcat ggtggtcgaa ggagtcggcg gcgtgcaggt acccatcacg cccagcgaca
      721 atgtgctgag cttgattaaa cacttgcgac ttccagttgt cgtcgttgga cgatccgggc
      781 tgggcggaat caatcatgct ttactgacga tcgaaaccct tcgccgacag aacatcagca
      841 tgatcgctct tgttctgaat cgcacacatc ccgtccgatc ggccctcgca cgcgttcaag
      901 agcgatcgac gcttgagatc cttcgcaaac aggccgggat acctgtgatc ggcccactcc
      961 cttacgaatc aggtctgcag agccgcttcc gccccgccat catcca
//
LOCUS       DASTWE010000168         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_941590, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000168 DASTWE010000000
VERSION     DASTWE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941590"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2571)
                     /gene="uvrA"
                     /locus_tag="VFT30_01880"
     CDS             complement(64..2571)
                     /gene="uvrA"
                     /locus_tag="VFT30_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5091403.1"
                     /translation="MGNSIIIKGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLFARVGRPYCFQCGDEITAQTVQQMVDAIASLPEGEKFQILAPIVRGRK
                     GEYRKELLEMRKAGYVRARVDGKIVDLGEDIALDKQKKHTIEIIIDRLVMKSGDALMR
                     RLADSVETSVKLTGGLVGVLTEDARTRLYSDKLACINCGVSYPEVTPRIFSFNSPHGA
                     CPACDGIGYAMPPGDQEEEDFTLLEPCQVCHGARLRPESLSIKLAKQSIAEVTSLSVR
                     AAADFFLSLKFTDRELVIAHRILKEIRERLGFLVNVGLDYLTLDRAAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVVVVEHDAETMQA
                     ADHLLDMGPGAGSHGGHVIAQGTPQQIMGDPNSLTGQYLRGAQTVSVPQRQRKPRGML
                     FIVGAQKHNLKNLTARIPLGLMTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDG
                     CKELRGVDALDKVIDIDQSPIGRTPRSNPATYTGLFGYIRDLYSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETLEILHKGKSIADV
                     LNMTVDDALEFFEHIPLIKAKLQTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSK
                     RATGRTMYILDEPTTGLHFADVQRLLDVLDRLVEAGNTVLVIEHNLDVIKNADWIIDL
                     GPEGGDRGGEIVAEGPPKEIAKARRSYTGQVLKEAGV"
BASE COUNT          608 a          868 c          753 g          675 t
ORIGIN      
        1 gttcggttgc tgtctcgagt catcgcatca tggcgacacc gagagccccg tcccttccag
       61 ctctcacact cccgcttcct tcagtacctg ccccgtatac gatcgccttg ccttcgcaat
      121 ctctttcggc ggcccttctg cgacgatctc gccgccgcga tctccgccct ctggtcccag
      181 atcgataatc caatcggcat tcttgatcac gtccaggttg tgttcgatca ccaacacagt
      241 gttccctgct tcgacaagcc gatccagtac atcgagcaat cgctgcacat cggcgaaatg
      301 cagtcctgtg gtcggttcat cgaggatata catcgtgcgg cctgtggccc gcttggagag
      361 ttcgcgtgac agctttactc tttgtgcttc gccaccggag agcgtcgtcg ccgattgccc
      421 cagctttaca tagtgcaggc cgacatcatg cagggtctga agtttcgcct tgatcaaagg
      481 gatgtgctcg aagaactcca acgcatcgtc cactgtcatg ttcaggacat cggcaatgct
      541 cttgcctttg tggagaatct ccagcgtttc gcgattgtag cgttgccctt tacacacctc
      601 acacgtcaca tacacatcgg gcaagaaatg catctcgatc ttgataagtc catcgccctg
      661 acaagcctcg catcgtcccc ctttcacgtt gaagctgtac cttccaggct tgtatccgcg
      721 aacccgcgac tccggaagat tggaatagag atcacggatg taaccgaaca ggcccgtata
      781 ggtggcggga ttggaccgcg gtgtacggcc gatcggcgat tgatcgatgt cgatgacttt
      841 gtcgagcgca tcgactcctc tcaattcttt gcacccatcg attttcggct tcttgtgata
      901 gagcaactgg gagagcgagt gaaagagtac ttccagcacc aaggtgctct tgcccgaccc
      961 cgatacgccg gtcacgcatg tcatcaggcc aagcgggatt cgcgccgtca ggttttttaa
     1021 gttatgtttc tgtgccccga ctataaaaag catgcctctt ggctttcgct gtcgttgcgg
     1081 cacggacaca gtctgcgcac cacgcaggta ttggcccgtc agcgagttgg gatcgcccat
     1141 gatttgttga ggcgtccctt gtgcaatgac atgcccgcca tgcgagccgg caccgggacc
     1201 catgtcgaga agatgatcgg cggcctgcat cgtctcggca tcatgttcta ccaccactac
     1261 cgtattgccg agatcccgca gtctcagcaa cgtctgtaac aaccggcgat tgtcgcgttg
     1321 gtgcaatccg atagacggtt cgtccagtat gtataagaca ccaaccaggc cagacccgat
     1381 ctgcgtggcc aatctgatcc gttgcccttc gccgcctgac agggtggccg cggctcgatc
     1441 gagcgtcagg tagtccaagc ccacgttgac cagaaagcct aatctctcgc gaatttcttt
     1501 gaggattcga tgcgcgatga ctagctcgcg gtcagtaaac ttcagcgaca gaaaaaaatc
     1561 cgccgccgcg cgaaccgaaa ggctggtcac ctcggcgatc gattgcttgg ctagttttat
     1621 agacaaactt tccggcctca gccgtgcccc gtggcagacc tgacagggtt ccaacagtgt
     1681 gaaatcctct tcctcctgat cacccggcgg catggcataa ccgataccgt cacaagcagg
     1741 acaggcgccg tgcgggctat tgaaggaaaa tatccgaggg gtgacttctg ggtaactcac
     1801 accgcaattg atacaggcta gcttgtcact atagagcctc gttcgagcgt cttccgttaa
     1861 gacgccgact aacccgccgg tcagcttgac cgacgtctca actgaatcgg ccagccggcg
     1921 catgagcgca tcgccggatt tcatcaccag ccgatcgatg atgatttcga tcgtgtgttt
     1981 cttctgttta tcaagggcaa tgtcttcacc cagatcgaca attttgccat cgacgcgagc
     2041 acggacatag cccgcctttc gcatctccag cagttccttc cgatattctc ccttcctccc
     2101 acgcacgatc ggcgccagaa tttggaactt ctccccttcc ggcaacgagg cgatggcatc
     2161 aaccatttgc tggacagtct gcgcagtgat ttcatctcca cattggaaac agtagggacg
     2221 gccgacgcgg gcaaagagca gccgaagata atcatagatt tccgtgaccg tgccgacggt
     2281 ggaccggggg ttgtggctgg tacttttctg ttcaattgag atcgcgggag acaggccttc
     2341 gatcgaatcg acatcgggct tgcccatctg ttcgaggaac tgccgtgcat aagccgacag
     2401 cgactccacg taccgccgct gcccttccgc gtatatcgtg tcgaaggcaa gggaggactt
     2461 gccggacccg ctcaagccgg tgatgaccac cagcttgtca cgcgggatct cgacgtcgat
     2521 gtttttgaga ttgtgctctc gcgccccctt gatgatgatg gagttgccca taagggcggg
     2581 attataccat ttgcgccgcc gtccaagttg atgaaagccc gaagatgggt tcagagatct
     2641 ccgaaattca gaagaaacca cgtttaagaa tatttgtcat gtctcaaacg agggcatgac
     2701 gagggtttcg cttgtacctt gtacgatgct tcgaggtgat gccgctcgta caaaggctcc
     2761 ccacccctcg ccttgacaaa gcttgaaacc gggtgctacc accgtcgcat ggtgaaaacc
     2821 gaccattctc cattggccca agattcccag gcccctgcca aggccgtctc tacatggtcc
     2881 ggccaggctc gcgaagctgc tgtg
//
LOCUS       DASTWE010000169         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1167680, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000169 DASTWE010000000
VERSION     DASTWE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167680"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFT30_01885"
     CDS             <1..469
                     /locus_tag="VFT30_01885"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091404.1"
                     /translation="EVWLVYMMALIEPLPERAVKDVLKRFPFSEQEATRLKMARVGCH
                     KVIRRLATQRTLKPAEVYHLLSGLSDETLLLLMAKSKGESVKRQVSAFLTTYQHVKSM
                     LTGRDLKTMGLKPGPQFKKILDQLIDARLNGEIKTESEERDLVMKLAALKMDS"
     gene            complement(466..1164)
                     /locus_tag="VFT30_01890"
     CDS             complement(466..1164)
                     /locus_tag="VFT30_01890"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HEU5091405.1"
                     /translation="MDTKQTPRMLTHDERKAAEAAFGGRPFNPAWSQSARKVYEGLTQ
                     ALPVLPGETVVPPHQNENASSEESPSPIEAAPTLPTIEARELEGPTPSGKEPEARTDT
                     PADQLITNRQQAIQAGFLIDVSTDAQKLGLTFPVTVTKPLWEVGIAPGESMSDEVKAE
                     RLRDVLMAFRLRIASQATLSPLIDFPAMLALPPGGVPQPVPLFALIQPDEQNRAAATL
                     LLPNEVSATIIPMN"
     gene            complement(1213..2478)
                     /locus_tag="VFT30_01895"
     CDS             complement(1213..2478)
                     /locus_tag="VFT30_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5091406.1"
                     /translation="MNITSWRSGVTSYQWLVLFVAWLGWVFDAMDATIYAIVLHPALH
                     DLLHTASGPPTAEQIGWYGGIIFSIFLIGWAIGGITFGVLADRFGRTKVLIATIIIYA
                     VFTGAAALAETWWHLAIARFLTALGIGGEWAAGASIVAETWPEEKRAKAAGILQSAWA
                     VGFFLAATMNLTLRDAYGWRGLFIIGILPAFVALLVRWWVKEPERWIHAHEQKAIPLT
                     AIFQGDLRRATFVGSTLAFVAVFGLWGATNWAPTLIRELPDLKGQEPATLTKYVSYAI
                     MALNAGAIFGYLGFGPLADRFGRRPIFAFMCLGSLIMLPVTYLTPSSYIGVLMLLPIL
                     GFFNNGIFSGFPIYLPELYPTQLRATGAGFCFNAGRVLASASPLLTGWLVATLGTFGR
                     AASTVALIYLLGLIVLLFAPETKGRRLPD"
     gene            2605..3234
                     /locus_tag="VFT30_01900"
     CDS             2605..3234
                     /locus_tag="VFT30_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5091407.1"
                     /translation="MNYYEVLGVSREASDEDIKKAYRKLVFEHHPDRNPHKKDANERI
                     REINAAYEIVGDPDKRKSYDRLSWGAEPRAETVDPGVVLDEMERKLFDEGRKELFAIL
                     MKDVKRVREELTLIREQVVAEQGYDSFLEHVVAARASAIMGDFVTEEMNGRKQRLVEV
                     AVEMMVSQGVAKRGDEGGIRALRNRLETAFRNGRVHGIASALELFYERR"
BASE COUNT          830 a          912 c         1007 g          696 t
ORIGIN      
        1 ggaagtctgg ttggtctaca tgatggccct tatagagccg ctgccggaac gggccgtgaa
       61 ggatgtgctc aagcggttcc ccttttccga gcaggaagcg actagactca aaatggcccg
      121 tgtaggctgt cacaaagtga ttcgtcgact tgcgacgcaa cgaacgctca aaccggcgga
      181 agtgtaccac ctcttgtcag gactatccga tgagacgctg ttactcctca tggccaagag
      241 caaaggagaa tcagtgaagc ggcaggtctc ggctttcctc acgacgtatc agcacgtgaa
      301 atcgatgctg accggtagag atctgaagac gatggggctg aagccggggc cacagttcaa
      361 gaagatcttg gatcaattga tcgatgcacg tctcaacgga gagatcaaga cagaatcgga
      421 agagagggat ttggtaatga agcttgctgc attgaaaatg gacagttagt tcatcggaat
      481 gatcgtcgcg gagacctcgt tgggcagcag cagggtggcc gccgcgcgat tctgttcgtc
      541 cggttgaatc agtgcgaaca acggcaccgg ctgcggcacg ccgcccggcg ggagggccag
      601 catcgcagga aagtcgatca gcggcgacag cgtggcctgg ctggcgatgc gaagccgaaa
      661 agccatcaac acatcccgca accgttcggc cttcacctca tccgacatcg actcaccggg
      721 ggcgatcccg acttcccaga gaggctttgt cacagtgacg gggaaggtca ggccgagctt
      781 ttgcgcatcc gttgacacat cgatcaggaa tcctgcttga atcgcctgct ggcgatttgt
      841 gattaattgg tcggccgggg tatcggttcg tgcctccggc tctttgcccg aaggagtcgg
      901 gccttcgagt tcccgtgctt ctattgtcgg taacgttgga gctgcttcga taggagaagg
      961 cgattcctcg cttgaggcat tctcattctg atggggtggc acaacggtct cgcccggcag
     1021 aacaggaagg gcttgggtca gcccttcata gacttttctg gcagattggg accacgctgg
     1081 gttaaacggt cggcctccaa aagccgcttc ggccgctttt ctttcgtcat gggtaagcat
     1141 tctgggagtc tgttttgtat ccatggtcgt tagataagct gtgagaatgg aaagtcaagg
     1201 actgaacata agctaatctg gtaaacgacg gcccttcgtt tccggagcga ataaaagcac
     1261 aataagtcca agcagataga tcagcgcgac ggtgctggct gcacggccga atgtgcctaa
     1321 tgtcgcgacg agccaaccgg tcaggagggg agaagccgac gccaagacgc gccccgcgtt
     1381 gaaacagaat ccggcccctg tcgcccgtaa ctgcgttggg tatagctccg gaaggtagat
     1441 cggaaatccg ctgaaaattc cattattaaa aaacccaagg atcggcagga gcatcagaac
     1501 gccgatataa ctcgagggcg tcaagtaggt gaccggcagc atgatgagac ttccgaggca
     1561 catgaaggca aatatcggtc gtctgccgaa cctgtctgct aagggaccga agccgaggta
     1621 gccgaagatc gccccggcgt tcaacgccat gatggcatag ctcacatact tggtgagggt
     1681 tgcgggctct tgtcccttca agtccggtaa ttcacggatc aacgtcggtg cccaattcgt
     1741 tgccccccaa agaccgaaca ctgcgacaaa ggccagcgtc gaaccaacga acgtggctcg
     1801 tcgtaagtcg ccttgaaaga tggccgtcag ggggatggct ttctgctcat gtgcatggat
     1861 ccagcgctcc ggctccttga cccaccagcg aacgagaagg gcaacaaacg ccggaagaat
     1921 gccgatgata aacaatcctc gccagccata ggcatctctc aacgttaaat tcattgtggc
     1981 ggccaaaaag aaccccacgg cccaagccga ttggagaatg cccgccgctt tggcacgttt
     2041 ctcttccggc cacgtctcgg ccacgatcga agcacctgcc gcccattctc caccgatgcc
     2101 cagcgcggtc agaaatctgg ctattgccag gtgccaccag gtctctgcca atgcagcggc
     2161 gccggtgaag acggcgtaga tgatgatcgt ggcgatcagg accttcgtcc tgccgaatcg
     2221 atcagcgaga acaccgaacg tgattccccc gatcgcccag ccgatcagga agatggaaaa
     2281 gatgataccg ccgtaccagc cgatctgctc ggcggtcggt ggaccactcg ccgtatgcag
     2341 caaatcgtgt aaggccggat gcaaaacaat ggcataaatc gtcgcatcca tcgcatcgaa
     2401 gacccagcct agccaggcaa caaacaagac caaccactgg taggacgtaa caccggatcg
     2461 ccaagacgta atgttcatgc gattctactg cgcctatcca ccaacaaaca ggccgggaca
     2521 gtgcgcgcaa ggctaagggg gttctccatt actgtcaagg tgaagcgtgc gacacctcgt
     2581 gctatagtcc ttcgcatgct gcggatgaat tactacgagg tgctcggcgt ctcacgtgag
     2641 gcgtccgacg aagacatcaa gaaagcctat cgaaagctgg tctttgagca tcatcccgac
     2701 cggaatcctc ataaaaagga tgcaaacgaa cggatcaggg agatcaatgc cgcgtacgag
     2761 atcgtcggtg atcctgacaa acgcaagagc tacgaccgtt tgtcctgggg agctgagcca
     2821 cgcgctgaaa cggtcgatcc cggcgttgtc ctcgatgaaa tggagcggaa gctcttcgac
     2881 gaaggcagga aagaattgtt cgcgatcttg atgaaagatg tgaagcgggt gagggaagag
     2941 ctaactctca ttcgagaaca ggttgtggcc gaacagggct acgactcgtt tctggagcac
     3001 gtagttgccg cacgcgcctc ggccattatg ggcgacttcg tgacagaaga aatgaacggg
     3061 cgaaaacaac gtctggttga agtggcggtg gagatgatgg tgtcgcaggg agtggcgaag
     3121 cgcggcgatg agggcgggat tcgagcgctg cggaatcgcc tcgaaacggc ttttcgaaac
     3181 ggtcgcgtgc acggcatcgc gtcggcgctg gagctgttct atgaacggcg atagcgccat
     3241 tggttaaaag gatctgcggc aactggtcaa aatttcaact cagtttcact gcttgccggg
     3301 tgggagacga gtcgtatccg cttcacatgc atcggttttt ctttcagtct gattagattc
     3361 acgcttcaga tcgaatcgga ccgctaatct ctctttctgt gtaagcaatt ctgtgaagta
     3421 gagtcagtcg cagcttcatc agaac
//
LOCUS       DASTWE010000170         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1297108, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000170 DASTWE010000000
VERSION     DASTWE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297108"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1028)
                     /locus_tag="VFT30_01905"
     CDS             complement(<1..>1028)
                     /locus_tag="VFT30_01905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5091408.1"
                     /translation="NATLRYLLSLLAVLSTGIYAQVYRYRHTTSPIKREQLKWIVFGI
                     AVGIFMGIGVNLFLMFFELTNPSASTYLIVDMVTQTLSVIAQFTVPVAVVFSILKYRL
                     YDIELVINRSLVYGLLTIILAAVFGVILFGLQAAYRAVTNQTNPPTIAVVVATVTVSS
                     LFQPTRKVLRRFINRRIYGIDVDFDELKRREEKREQLALMPSQGVTSVGGYKDLELIA
                     RGGMGEVYKARHPNLNRTVAIKVLSAFFKDDPGFNKRFAREAKTMAQLRHPNIITIHD
                     FGDQKGLPFIVMEYLTGETLSQILARQGCLALDEGLPLLQDLASALDYAHQQGVIHRD
                     IKPSNVII"
BASE COUNT          232 a          265 c          315 g          216 t
ORIGIN      
        1 cgatgatgac gttggacggt ttgatgtccc ggtggatgac tccctgctgg tgggcataat
       61 ccagggcaga tgccaggtcc tggagcagag gcagtccctc gtcgagggcg agacaccctt
      121 gtctcgccag gatctgtgag agggtctcgc ccgtcaggta ttccatgacg ataaagggca
      181 atcccttctg atcaccgaaa tcgtggatgg tgatgatatt gggatgtcgc aattgcgcca
      241 tggtctttgc ctcgcgggcg aagcgcttgt tgaagcccgg atcatccttg aagaatgccg
      301 acaacacttt gatcgcgacc gtccggttca ggttcgggtg ccttgcctta tagacctccc
      361 ccataccgcc gcgcgcgatc aattccaggt ctttataacc cccgacacta gtcaccccct
      421 gggagggcat aagcgcgagt tgctccctct tttcctcgcg gcgtttgagt tcgtcgaagt
      481 cgacgtcgat gccgtagatt cggcggttga tgaagcgacg aaggaccttg cgagtcggtt
      541 ggaaaagact ggagaccgta accgtggcga caacgaccgc gatcgtcggt ggattggtct
      601 ggtttgtgac agctcgatag gcagcctgca agccaaagag gatgacgccg aagacggccg
      661 ccaggatgat cgtcagcaaa ccatacacga gggatcggtt gatgaccagt tcaatgtcat
      721 agaggcgata tttcagaatg gaaaagacca cggcaacggg caccgtaaat tgtgcaatga
      781 ctgagagcgt ttgcgtgacc atgtccacga tgaggtaggt cgaggcggag gggttcgtca
      841 gctcgaagaa catcagaaag aggttgacgc cgatgcccat gaaaatgccc acggcaatgc
      901 cgaagacaat ccacttaagt tgctcgcgct tgatcgggct ggtcgtatgc cggtagcggt
      961 agacctgcgc gtaaatcccg gtgctcaaca cggcgagcag tgagagcaga tatcgcaacg
     1021 tggcgttc
//
LOCUS       DASTWE010000171         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_263141, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000171 DASTWE010000000
VERSION     DASTWE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263141"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2411
                     /locus_tag="VFT30_01910"
     CDS             <1..2411
                     /locus_tag="VFT30_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091409.1"
                     /translation="PPEKVIGHLRLGLSEERLRTELYGYLLWIGSLTFAVVLVGVMVT
                     LLLIRRIIHPVQDLVEATGRIAGGDFRQSVVVTSNDEIGTLAESFNGMIQRLATSQEE
                     VQQYQRTLEAKVEERTKELEEKTHEAVELAQKAQAASQAKTEFLANMSHEIRTPLNGV
                     LGMTQILLHTQLTDKQRRFVEIVDKSGITLLDVVNDILDFSKIEAGKLQLENSDFDLQ
                     ELIGEVMDLFAHRVQSKQVELASNIIDEVPTHLRGDVTRLRQILVNLVGNAVKFTEQG
                     EIVLSVSQVEEKADQVTLRLEVKDTGLGIAPSVQEKIFEAFSQADGSTTRKFGGTGLG
                     LTIVKQLVSLMNGTVGVESSLGDGSTFWLEIPFSRSLEGESKRWEACDAFRRMRAIVV
                     DDNATSRHILDQYLNGWGIAHVTVSSGKEALEVIRNVSDPQALYDLAIIDGQMPEMAG
                     FELARAIRADRRLARIKIIMLTSMEQDERLLASEAAGELDGSITKPVRQSHLYDLLVA
                     LTVTGNGSSALPEPVPVERSHPEPRKDVSVLVAEDNPVNREVTAEMLAVEGYQVHTVC
                     NGREAVDAIAARMFDLVFMDCQMPEMDGFAATAAIRARERNLKLPRIPIVALTAHAIA
                     GDRERCLAQGMEDYLSKPFRQSQLIAVVRRWVSEGRSSSTNKPDTTNPATPLVAALSE
                     SVDCLDERILRNLRDLRRPGRPDIYVDMLSRYLDSSRQHIETMRQHIVAQNAAALFQT
                     AHSLKSSSGMVGARTLAEHIKRLEVLGNSGDVSSAPAVFAQVEMEYHRVKRAIEGLLG
                     KEAA"
     gene            2679..2825
                     /locus_tag="VFT30_01915"
     CDS             2679..2825
                     /locus_tag="VFT30_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091410.1"
                     /translation="MPVRDGFSVCAKLRESGDGSRVPILIMTGLDDADSIAGHTSSGR
                     LISS"
BASE COUNT          746 a          735 c          933 g          711 t
ORIGIN      
        1 agcctccaga aaaagtgatc ggacatctga ggcttgggct cagcgaagag cgattgagga
       61 cagaactcta tgggtatctg ctctggatcg gatccctgac atttgccgtc gtattggtcg
      121 gtgtcatggt gaccttgctt ctgattcgtc ggattattca tcccgttcaa gacctcgtcg
      181 aagcaactgg gcggatcgcc ggaggagact ttcggcaatc tgtggtggtg acaagcaatg
      241 atgaaatcgg gacgttggcg gaatccttta atgggatgat ccagcggttg gccacctctc
      301 aggaggaggt ccagcaatac cagcgtaccc tggaagcgaa ggttgaagag cggacgaaag
      361 agcttgaaga gaaaacgcat gaggccgtgg aattggctca gaaagctcag gcggcaagtc
      421 aggcgaagac agaattttta gccaacatga gtcatgagat tcgaactccg ctgaacggag
      481 ttctgggtat gacacagata ttgcttcata cccagttgac ggataaacag cggcggtttg
      541 tcgagattgt ggataaatca ggcatcaccc tactcgacgt ggtgaatgat atcctggact
      601 tctccaagat tgaagcgggg aaattgcaac tagagaacag cgacttcgat ttgcaggagt
      661 tgatcgggga agtgatggat ctcttcgcgc accgagtgca gtcgaaacag gtcgagttag
      721 cctccaatat tatcgacgag gtgcctacgc atttgagagg agatgtcacg cgactgcgcc
      781 aaatcctcgt gaatcttgtg gggaatgccg tcaagtttac agaacaaggg gaaattgtgc
      841 tgtcggtatc tcaggtcgaa gagaaggccg accaagtcac cctccgccta gaggtaaagg
      901 atacgggatt aggaatcgcc ccttccgtac aagaaaagat cttcgaggcg ttctcgcagg
      961 cggatgggtc aaccacgagg aagttcggtg ggacggggct gggcttaacc atcgttaaac
     1021 agttggtgag tctgatgaac ggaacggtcg gtgtcgaaag ctccttggga gatggttcga
     1081 cgttttggtt agagattccg ttctctcggt cgctcgaggg cgaatcgaaa cgctgggagg
     1141 cctgtgacgc attccgccgg atgcgcgcga tcgtggtcga cgacaatgcg accagccgcc
     1201 acattcttga ccaatacctg aatggatggg gtattgccca cgtgactgta tcgtccggca
     1261 aagaagcgtt agaagtcatt cgtaatgtgt cggatccaca ggcactgtac gatctggcga
     1321 ttatcgacgg gcaaatgcca gaaatggccg ggtttgaatt ggccagagcc atacgagctg
     1381 ataggcggct ggcgaggata aaaatcatca tgctgacctc gatggagcaa gatgaacgat
     1441 tgcttgcctc agaggctgcc ggggagcttg atggttcgat taccaagccc gtgcgtcaat
     1501 cccatctcta cgatcttctc gttgccctca ccgtgacagg gaatgggagt tcggctctgc
     1561 ccgagcctgt gcctgttgaa aggtcccatc cggaaccccg gaaggatgtg tctgtcttgg
     1621 tggcagagga caatccggtg aatcgagaag tcacggcgga gatgctcgcg gtcgaggggt
     1681 atcaggtcca taccgtctgc aacgggcgcg aggctgttga tgcaatcgct gcccgcatgt
     1741 ttgatctcgt gtttatggat tgccagatgc ctgagatgga tggatttgcc gccaccgccg
     1801 ccatccgtgc tcgcgaacgg aacttgaagc tcccgcgaat acctattgtt gccttaaccg
     1861 cccatgccat tgcaggagat cgagagcgct gtttagctca aggcatggag gattatttga
     1921 gcaagccatt ccggcaatct caactcattg cggtggtgcg acgatgggtt tcagaaggta
     1981 ggtcgtcttc tacaaacaaa cccgacacga ccaatcctgc tacccccctc gttgctgcct
     2041 tgagcgagtc tgtcgactgc ctagacgaaa ggatcttgcg gaacttgcgc gaccttcgtc
     2101 gccctggaag accggatatc tacgtcgaca tgttgtctcg atacctcgac tcctcaagac
     2161 aacatattga aaccatgaga cagcacatcg tcgctcagaa cgccgctgcg ttgttccaaa
     2221 ctgctcattc tcttaaatca agcagcggga tggtcggtgc cagaacattg gccgagcaca
     2281 taaaacgact cgaagtattg gggaattcgg gggatgtgtc gtcagcaccg gctgtgttcg
     2341 ctcaggtaga aatggaatat caccgtgtga agcgagcgat agagggatta ttggggaaag
     2401 aggctgcgta agtcgcttat ggctgtcatt cgaacggtct ttgtccgcct gttgtcgaat
     2461 gtagtggatt actaaaaaca gtagagattc tgacatgcgt aatagagacg agacaacttc
     2521 ggcattagtc ctggttgtag atgacgaaga gttagccaga ttattcgtcc gtgaagcgct
     2581 ggaacagcag gattcgaggt gtgcgaagcc tcgaatggct tgcaggcgct cgaacagttt
     2641 gcgtgtctgc ggccggatct tattgtaatg acgtcgtgat gcctgtgagg gacggatttt
     2701 ctgtctgtgc gaaacttcgt gagtcgggag acgggagccg tgtgccgatc ctgatcatga
     2761 ccggtttgga tgatgccgac tcaattgccg ggcatacgag ttcggggcga ctgatttcat
     2821 cgtaaaacca atgaatgcaa gcatcatagg gcatcgggta cggtacatgt tacgggggag
     2881 catcacgctg aatgctttgt tgcgcagcga ggcccggctt ggcctggcgc aacggacata
     2941 ttcggtcggc ctatgccagg tgaagaattt gtggaactgc tcgtacggac cacctctccg
     3001 tcgtgcaaca gacgggcggc ataggctcaa gagcagcagt aacggaatcg tgttcgatcg
     3061 gcaatataaa gggctagcca cacacggtcc tgtttgcctg gaacaaagga agatgctcag
     3121 ttcac
//
LOCUS       DASTWE010000172         5307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_489346, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000172 DASTWE010000000
VERSION     DASTWE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489346"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..594)
                     /locus_tag="VFT30_01920"
     CDS             complement(277..594)
                     /locus_tag="VFT30_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091411.1"
                     /translation="MNFQNSIDSGAIDSGSNDLSVFKPPTGTVSGTTGQICSRTGVYR
                     STDNKAQYLEVVAAGDPFPPFPGGKGTSKTTWTLMTSSNSGTAATTEDGGFRSVLVEA
                     GTA"
     gene            complement(696..1331)
                     /locus_tag="VFT30_01925"
     CDS             complement(696..1331)
                     /locus_tag="VFT30_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091412.1"
                     /translation="MKRIILVVSILIAFGGLEVARAQTTPRLPDEKQVAQPKKRGGLI
                     TLYALDPLVRTLCFSDGKEGMAIDKNRWGNRCSDLSFSLAGNGSFVTGIEVNRVAAIV
                     DLGTANDLRGRYGYEDADNGGVGFASLRLDGGKIMILKDDNVQEEYQPLTEGPKLFTD
                     VGPSANAPIKLGHIYLVRLADQKDKSFQQIVKLIVVAYRPEEAVTFRWELL"
     gene            complement(1374..2321)
                     /locus_tag="VFT30_01930"
     CDS             complement(1374..2321)
                     /locus_tag="VFT30_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091413.1"
                     /translation="MKILAIFALIVLTFTGTAFAQEKSCSFNLVGTWKVQLTKTDARL
                     YTFDDSGNVKILEVAGTAEPKEIATAKYSIDPETPEVVSFKASGKNRIFGRAKATMTV
                     EKYDDVSLTCSIPGAGTTRWTKVDPNRYFIVLAARTEEFYDHTGSAFPVLIKLSNGAP
                     EINAVGIYSDEGKPHFGTVPPETYKNYLREARGDSETVLRLEINGAQYDRSLMVVKEW
                     QRRAREDALLYRPPTSPVTLNNILLVKAVTETLNQCSDDVSLYKLNYVHPQDWLTDKY
                     SPELVPFYYFKELRRLNEARHISDQKFQEIIPSNNLAVR"
     gene            complement(2474..3112)
                     /locus_tag="VFT30_01935"
     CDS             complement(2474..3112)
                     /locus_tag="VFT30_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU5091414.1"
                     /translation="MDRRTMLNKVFEPHPASPGITRYTNAVLRTQDNQEFRFYDDLIR
                     GKQVVINFMYAECHGACPLVTKTLITTYNTLKDRMGKDLFFYSITTKPKDDTPTALKH
                     YAQMRKADLPGWYFLTGDSYDIETIRFRLFNMNHPGFDLDTAMHAGSLRIINDATNTW
                     LMAEAFASQKAILKRISWADPQKSYAERVVESRARQVLINADIKKYGYRRDV"
     gene            complement(3112..5043)
                     /locus_tag="VFT30_01940"
     CDS             complement(3112..5043)
                     /locus_tag="VFT30_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5091415.1"
                     /translation="MPKKKAKNRQGIGDEETKGTISRRNVLKIGAAAGAATVLAPNVL
                     TSNNALAFQDPIPSEPVICVTPANSPPHHPFVDEFTAPFPAVDTPLNPQPTEFANLGA
                     GEADRAPHPRWAQFTPAHFTYEMKARPGLHTFHSDFSPTYIWGFNGKYPAPTCLQRYG
                     KPTIVRFHNNLPVTTTTFGRNEITIHLHNGHHGSESDGFAGDFFGTGFWKDNHYLNCY
                     AGLNAFGGNGDPKEKMGSFWFHDHRAEFTLNNNVLGLNGMYLVYDPTDPGHEHPSAGS
                     LQLPGYYGVTDFPVILVDHRFCPTADGRTELVNAAGGDKFIVNGKIQPKMTVRRRKYR
                     FRFLNTGPTQTYDITLIKPDGSVGTMVVIATDANFLQNPIPIDAGANAGNSNSTTAPI
                     VPGGLRVSVAERYDVIIDFGAFPAGSKLYLKENRAMFVGNPSPDPLPPGLAIENVLMQ
                     FNVVNTEAWFPRDTPAIPSTLCTYPTLPATDATFEWQFVRDPVGSVPRLFRINGFAFD
                     PEIPRHCILQDTCEEWLLNNNVGGSAWVHPVHIHFEEFRTLKRFVNGVEVPVPPLQSG
                     RKDVSRLEAGQGALIKMQFRDISGRYLIHCHNMAHEDAFMMVRWDIVPDAAAKAACEA
                     LMAQGRKELESKLRNEEVA"
BASE COUNT         1137 a         1395 c         1451 g         1324 t
ORIGIN      
        1 aggtaacgtc ttcgtgcatg gggcagacat agtggaattt gagcggcttt ttaggatgcg
       61 ctgagcgttc cttctgtgct gcggtgacga cggccacaga gtacaacggg gaaccaatta
      121 gaagcaatgc cgccgcgacg agcggcacga tcagaagccg aaggaaattc attactaccc
      181 ctggataaag actcttcctg ctcaagccgc taacgacatg agcaggaaga gctatgttat
      241 tgacgccgag acttgaagga agaggtggtg ctctccttag gcagtccccg cctcgaccaa
      301 cactgatcta aagccgccat cctcggtagt ggcagcggta cccgaattgg acgaggtcat
      361 cagggtccag gtcgtcttcg aggtcccttt gcctcccgga aacggcggga aaggatcgcc
      421 ggccgcaact acttcgaggt attgggcttt gttgtcggtg gagcgataga caccggttcg
      481 cgagcagatt tggccggttg ttccgctaac cgttcccgtc ggtggtttga aaacgcttag
      541 atcattcgat ccggaatcaa ttgcacctga gtcgattgag ttttgaaaat tcataccagt
      601 tctccttcaa gttgaagttc gtaaacctgc ttccttcgag ggaagcgcga aatttgatca
      661 gtcgccacgc actgctaagt tagaccacag ccggattaga gcaactccca tcggaacgtc
      721 actgcttctt caggacgata agcaacgacg atcagcttga cgatctgttg aaaactcttg
      781 tccttctgat ccgccagacg cacgaggtag atatgtccca gcttgatcgg tgcgtttgcg
      841 gatggtccaa cgtcggtaaa aagtttgggc ccctcagtca gcggctgata ttcctcctgg
      901 acgttatcgt ctttgaggat catgatcttg ccgccgtcta aacgtaacga agcaaaacct
      961 actccaccgt tgtccgcgtc ttcgtagccg taacgtccac gcagatcgtt ggcggtgccg
     1021 agatccacga tggcggcgac gcggttgact tcaatgcctg ttacaaagct gccattacca
     1081 gccaggctaa aactaagatc gctgcaacgg ttaccccagc gattcttatc aatcgccatc
     1141 ccctctttcc cgtcactaaa gcagagtgtg cgtaccagag gatcaagcgc atagagtgtg
     1201 atcagccccc cgcgtttctt tggctgagcc acctgtttct cgtccgggag gcgcggtgtt
     1261 gtttgcgcgc gcgctacctc gagaccgccg aacgctatca gaatcgagac gaccaaaatt
     1321 attcgtttca cagtaattct tctcccgtct tgcttatttg tactgctccg tatttaacgc
     1381 acggccaggt tgtttgacgg gattatttcc tggaattttt gatcgctaat gtgccgggct
     1441 tcgttcaatc gtctcagctc tttgaagtag tagaacggaa ccagctccgg gctgtactta
     1501 tcagtgagcc aatcctgtgg atgaacgtaa ttgagcttgt agagactgac gtcatccgag
     1561 cactggttaa gtgtttccgt gactgccttc acgagcagaa tattgttaag agtgacgggg
     1621 gaggtgggcg gccggtacag gagcgcatct tcccgggcgc gacgttgcca ctctttgact
     1681 accatcaagc tacggtcgta ttgcgcaccg ttgatttcaa ggcgcaacac tgtttccgaa
     1741 tcaccgcggg cttctctcag ataatttttg taagtctctg gcggaacggt tccgaagtgg
     1801 ggcttgccct catcggaata aatgccgacc gcgttgatct ccggggcacc gttggagagt
     1861 ttgatcaaga ccggaaaagc tgatccggtg tggtcataaa actcttccgt gcgcgcggcg
     1921 agcacgatga aataccggtt cggatcgacc ttggtccatc gtgttgttcc tgctcccgga
     1981 atagagcagg taagcgaaac gtcgtcgtat ttctcgactg tcatcgtcgc tttggcgcga
     2041 ccgaaaatcc tgttcttgcc gcttgcttta aaggagacga cctccggagt ttcgggatcg
     2101 attgaatact tggcagtcgc aatctccttc ggctcagccg tgccggcaac ctcaagtatc
     2161 ttcacgttac cgctgtcatc gaaagtgtaa agacgagcat cagttttcgt gagctggacc
     2221 ttccacgtgc ccactaagtt gaaagaacaa ctcttttcct gggcgaacgc tgtacccgtg
     2281 aaggtcaaaa ctatcagagc gaatatagcg agtattttca tgatgaatga ccctttgatg
     2341 tgcccgagcc tatgcgctcg gtcttagtgt tgggccagga atagaagccg catgagcggc
     2401 ctctattccc ggtttgtgct atgaccgtcg agggcgggct gactccagcc tggtgcgacg
     2461 gcctggccgc aaactacaca tcccgacgat agccgtactt cttgatgtcg gcgttaatca
     2521 ggacctgacg agcccggctt tccacgactc tctccgcata tgatttctgc ggatcagccc
     2581 aggaaatccg tttcaggatc gctttctggc tggcaaacgc ctcggccatc agccacgtgt
     2641 tggtcgcgtc gttgatgata cgcagcgagc cagcgtgcat ggcggtgtcg agatcgaagc
     2701 caggatggtt catgttgaag agcctgaagc ggatggtctc gatatcgtag gaatctccgg
     2761 taaggaaata ccagccaggg agatcagcct tgcgcatttg agcgtagtgc ttcaacgcgg
     2821 tcggtgtatc gtcctttggc tttgtcgtta tcgagtaaaa gaagaggtct ttacccatgc
     2881 gatctttgag ggtgttgtag gttgtgatca aggtcttggt gaccagcggg cacgctccgt
     2941 gacactcggc gtacatgaaa ttgatcacga cctgcttgcc tcggatcaga tcgtcataga
     3001 accggaactc ttggttgtcc tgcgttcgga gcacagcgtt tgtgtaccgc gtgatgcccg
     3061 gtgatgcggg atgcggctcg aacactttgt taagcattgt tcttctatcc attacgccac
     3121 ctcctcattc cggagcttgg attcaagttc cttgcgaccc tgggccatca atgcctcgca
     3181 agctgccttg gctgctgcat ccggtacgat gtcccagcgc accatcatga acgcgtcctc
     3241 gtgggccatg ttgtggcagt ggatcagata ccgaccagag atgtctctga actgcatctt
     3301 gataagagcc ccttgtcctg cttcgagacg gctgacgtcc tttctccccg attgcagcgg
     3361 cggcaccgga acttccacac cgttcacgaa tcgtttcaac gtgcgaaact cttcgaagtg
     3421 aatgtggacg gggtggaccc aggcggagcc accgacgtta ttgttcaaga gccactcttc
     3481 gcaggtgtcc tggagaatgc aatgccgcgg tatttccgga tcgaatgcga atccattgat
     3541 cctgaacaag cgcggtacac tgcccacagg gtcacggaca aactgccact cgaaggtagc
     3601 atctgtcgcc ggcaacgtcg gatacgtgca gagagttgac gggattgccg gagtgtctct
     3661 cgggaaccac gcttcagtgt tcaccacgtt gaactgcatc aggacgttct caatggccag
     3721 tcctggcggc agcggatcgg gtgacggatt gccgacaaac atcgccctgt tttccttgag
     3781 gtagagcttg gagcctgccg ggaacgctcc gaagtcgatg atgacatcgt aacgttcggc
     3841 cacgctcact cggagaccgc ccggaacgat tggcgccgtg gtgctgttgc tgttgccggc
     3901 attcgcacct gcgtcgatcg ggatcgggtt ctgaaggaag ttcgcgtcag tggcgatcac
     3961 gaccatcgtt ccgactgagc catcgggttt gatcagggta atgtcgtaag tctgtgtcgg
     4021 tccggtattg aggaagcgga acctatactt tcgccgcctc acagtcatct tcggctgaat
     4081 ctttccattg acgatgaact tatcgccacc ggccgcattg accagctcag ttcgcccatc
     4141 agcagtcgga cagaacctgt ggtccacaag tatcactgga aagtcagtaa caccataata
     4201 tcccggcaac tgtagtgaac ctgccgatgg gtgctcatgg cctggatccg ttggatcgta
     4261 cacgaggtac attccgttca agcccagcac gttgttgttc agcgtgaatt ctgcgcggtg
     4321 gtcgtggaac cagaatgaac ccatcttttc tttggggtca ccatttccac caaaagcgtt
     4381 caaccctgcg tagcaattca ggtagtgatt gtctttccag aacccggttc cgaagaagtc
     4441 acctgcgaaa ccgtcgctct ctgagccatg gtgtccgttg tgcaagtgga tcgtgatctc
     4501 gttcctgccg aacgtcgtcg ttgttaccgg caagttgtta tggaaacgca cgatggtggg
     4561 cttgccatat ctttgcaggc aggtcggcgc tggatacttc ccgttgaagc cccagatgta
     4621 cgtcggagaa aagtccgagt ggaacgtatg aaggccgggc ctggctttca tttcatacgt
     4681 aaagtgagcc ggggtgaact gcgcccagcg tggatgcggc gcgcgatcgg cctcacctgc
     4741 acccaggtta gcgaactcag ttggctgcgg gttcaacgga gtatcgaccg caggaaatgg
     4801 tgctgtgaat tcatccacga agggatgatg tggtggactg ttagctgggg taacgcaaat
     4861 taccggttcg gacggaatcg gatcctggaa ggccagtgcg ttgttagaag tcagtacgtt
     4921 tggggccaaa acagtcgcgg ccccggctgc tgctcctatc ttcagcacat ttcgtctact
     4981 gattgtgcct ttagtttcct catctccaat tccctgccgg ttttttgcct tctttttagg
     5041 catagaatct cctttcagcc gtttgggttt ggcctcaatc taaaatccct tgctggtggt
     5101 tatgaccttt actcgaagag tgtagctatg tggctcccga atcctgatct aacttcccga
     5161 caaaggcgat cagtttgaaa ggcgagggct tctgctgaaa ccagttgagt aacgtttatt
     5221 gactacacga aattaacgaa tggtgaaagt tcacttgtgc gctgattcgt gtgaaagatt
     5281 acgatctgaa cttttgactt acaagac
//
LOCUS       DASTWE010000173         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1232608, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000173 DASTWE010000000
VERSION     DASTWE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232608"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFT30_01945"
     CDS             <1..757
                     /locus_tag="VFT30_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091416.1"
                     /translation="RERWLEAGVTMRDPCSVWIDAEVTIGRDTVLYPHVSLEGKTIIG
                     EGTIIRSGVRISSCVIGNNVEILDHCVLRESQVDDEAHLGPFAHLRPGAIVRRKAKVG
                     NFVEMKKAELGEGSKANHLTYLGDARIGKGVNVGAGTITVNYDGANKHQTLIEDYASV
                     GSDTQIIAPITIGQGAIIAAGTTITQDVPADSLAISRVLQVNKVGWAAKRRMLAGGMP
                     SRDSARASGELTEMKTDNTASKVKKGRAKSSKR"
     gene            817..>2243
                     /gene="glmS"
                     /locus_tag="VFT30_01950"
     CDS             817..>2243
                     /gene="glmS"
                     /locus_tag="VFT30_01950"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5091417.1"
                     /translation="MCGIIGYVGNQDAVPILIGGLAKLEYRGYDSSGVAVMQGEKIAV
                     RRSVGKLINLQNSLKTNELKGTIGIGHTRWATHGKPSEQNAHPHRSKGCVLVHNGIIE
                     NYQALKQQLQKDGYKFESETDTEVVAHLIDKYLQKENKLADAVRLATKDVRGSYALAV
                     ISEREPGTLIAARSGCPLVVGRTKHASYVASDVMAMLAHTREVTYLEEGDVAVVTQHR
                     VDLTDVDGHAVSRKSSTITWDASAAEKSGYPHFMLKEIHEQPQTILDTMRGRYSYETG
                     EADLPDIGLTPKEFAAVERIWIVACGTSWHAGQVGKYLFEEMVRTPVQVDIASEFRYR
                     DPLVGKNDLFITISQSGETADTLAAAREAKQKGARVVSIVNVVGSTLARESDGILYTH
                     CGPEIGVASTKAFTAQLTALYLLALHFARVRNVMKVADGKAWLDRLVQLPVLVERVLQ
                     REAEIVAIAKRFYKKRNFLFLGRGI"
BASE COUNT          548 a          553 c          693 g          449 t
ORIGIN      
        1 tcgcgaacgc tggcttgaag ctggagtcac gatgcgagat ccttgttccg tgtggattga
       61 tgcagaagtc actatcggaa gagacacggt gctctatccg catgtgagtc ttgaaggcaa
      121 gacaattatc ggtgaaggaa cgataatccg ttccggcgtc cgtatttcaa gttgcgtgat
      181 cggaaacaat gtagagattc ttgatcattg cgtcctacgc gaatcgcaag tcgacgacga
      241 ggctcatttg ggaccattcg cgcatttgag acccggggcg atcgtacggc ggaaagcgaa
      301 agtcggaaac ttcgtcgaga tgaagaaggc cgaactcggc gaagggtcca aggcaaatca
      361 cctgacatat ctcggcgacg cgcgaatcgg gaaaggtgtc aatgtagggg ccggaacaat
      421 cacggtaaat tatgacggcg cgaacaaaca tcagaccctg atcgaggatt atgcgtctgt
      481 ggggagcgat acccagatca tcgcgccaat caccattgga caaggagcaa tcattgcggc
      541 cggtacgacc atcacgcaag atgtgccggc tgattcattg gcgatctccc gtgtcctgca
      601 ggtgaataag gtggggtggg cggctaaacg ccgaatgttg gcaggcggga tgccgagcag
      661 ggactcagcg cgagcttcgg gtgaactgac tgagatgaaa accgacaaca ctgcaagcaa
      721 agtgaagaag gggcgcgcaa agtcatcgaa acgctgatca cgtaagagag gcaatcgtcg
      781 tctgtcggtc agctagtcaa gtctggagca accgtcatgt gtggaatcat cggatacgtc
      841 ggcaatcaag atgcggttcc gatcctcatc gggggactgg ccaagttgga gtaccgaggg
      901 tacgactcct cgggagtcgc cgtcatgcag ggagaaaaga ttgctgtcag gcggagcgtg
      961 ggcaaattaa tcaacctcca aaattcgctc aaaaccaacg agctcaaggg aacgatcgga
     1021 atcggccata ctcggtgggc aactcatgga aagccctcgg aacagaatgc ccatccacat
     1081 cgatcgaaag gatgcgtgtt ggtgcacaat gggattatcg aaaactatca agcgctgaag
     1141 cagcagttgc aaaaagacgg ctacaaattc gagtcggaaa ccgatacgga ggttgtcgcc
     1201 catttgatcg ataagtatct tcagaaggaa aataagctgg ccgatgccgt gcggctcgcg
     1261 acaaaagacg tgcgaggcag ttatgcgctg gctgtgattt ccgaacggga gcccgggacg
     1321 ttgatagccg cgcgatccgg ctgcccactc gtcgtcggtc ggacgaagca cgcgtcctac
     1381 gtcgcgtccg atgtgatggc gatgctcgcg cacacgcgag aagtgaccta tctcgaagag
     1441 ggggacgtgg cggtggtcac gcaacaccga gtggacctca cggacgtcga cggccatgcc
     1501 gtctcgcgta agtcgtcgac gattacatgg gatgcctcgg cagcggagaa aagcggctat
     1561 ccccacttca tgctgaaaga gattcacgaa cagccgcaga ccattcttga taccatgcgc
     1621 ggacggtatt cctatgaaac gggcgaggcg gatcttcctg atatcggcct gacgccgaaa
     1681 gaattcgcag cggttgagcg catctggatc gtcgcctgcg gcacgtcctg gcatgcgggt
     1741 caagtcggaa agtatctgtt cgaagagatg gtgcgtactc cggttcaggt ggatatcgcc
     1801 agcgagtttc ggtatcgcga tccgctcgtc gggaaaaatg acttattcat tacgatttct
     1861 caatccggtg agacggccga tacgctggcc gctgcgcgag aggcgaagca aaaaggtgcg
     1921 cgcgtcgtct caattgtcaa cgtcgtcggc agtactttgg cgcgtgagtc ggacgggata
     1981 ctgtacacgc actgcgggcc ggaaatcggg gttgcctcca ccaaggcgtt taccgcgcaa
     2041 ctcaccgcgt tgtatctcct ggccttgcat ttcgcgcgag ttcgtaatgt gatgaaggta
     2101 gcggacggga aagcctggct ggaccgattg gtgcagttgc cggtgttggt cgagcgtgta
     2161 ttgcagcgag aagccgaaat cgtggcgatc gccaagcggt tttacaagaa acgtaatttt
     2221 ctgtttttgg gccgcggcat caa
//
LOCUS       DASTWE010000174         1237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_457037, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000174 DASTWE010000000
VERSION     DASTWE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457037"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFT30_01955"
     CDS             <1..1136
                     /locus_tag="VFT30_01955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5091418.1"
                     /translation="VTTLFLVIVADKILRVAGLSLLDRVGSTRYILAYATSLTLIGLW
                     ITTAWLLHLDALHRFFVKSVQSTRQQEPGRREESTDEIDPKQKSSKSATHILTTQNSP
                     PSTLGRYKVLKELGRGSMGVVYLGKDPTIQRFVAIKTMRLDEIDDRDKLQEVKARFFR
                     EAESTGRLSHPNIVTIFDAGEEDDLGYIAMEMLQGTTLTQWSRKPNLLALEKVISILA
                     TVAEAMDYAHQQGVVHRDIKPANIMLTTDTVVKIMDFGIAKMATSSKTQTDIVLGTPM
                     YMSPEQIAGKKVDGRSDIFSLAVVMFELLTGRLPFTADNVSALLFAIAHTPHLSVKAI
                     RPDLPPTIQEVLDRALQKDPVHRYRRAGEFAAELRSCLQGLAA"
BASE COUNT          295 a          355 c          329 g          258 t
ORIGIN      
        1 ctgttacgac cctcttcctt gtgatcgtgg cggataagat tctgcgggtt gccggacttt
       61 cgcttctcga ccgtgtggga tccacacggt atatcctggc ctacgctacg tcattgacgc
      121 tgatcgggct ttggatcacc actgcatggc ttctgcatct cgatgccctt caccgttttt
      181 tcgtgaagtc ggtgcaatcg actcgacaac aagaacctgg acggcgcgaa gaaagtaccg
      241 acgaaatcga tccaaagcag aaaagcagca aatctgcaac gcatatcctc accacacaga
      301 acagcccacc aagtacactc ggccggtaca aggtactaaa ggagttagga cgcggctcga
      361 tgggggtggt gtatctgggg aaagatccga cgatccaacg attcgtggcg atcaaaacca
      421 tgcgactcga tgagatcgac gaccgagata aactgcagga ggtgaaggcg cggttctttc
      481 gggaggccga atccaccgga cgattgtctc accccaacat cgtcacgatc ttcgatgccg
      541 gagaagaaga cgacctcggc tacattgcca tggaaatgct ccagggcacc acgctcacgc
      601 aatggtcaag gaaaccgaat ctcctggcgc tcgaaaaagt gatctcgata ttggcaaccg
      661 tcgccgaagc gatggactat gcccaccagc agggggttgt gcatcgcgac atcaaaccag
      721 cgaacatcat gctgaccacc gatacggtcg tcaagatcat ggacttcggt atcgcgaaga
      781 tggcgacgtc gtcgaaaact caaacagata tcgtgctggg aactcccatg tacatgtccc
      841 ccgaacagat cgccgggaag aaggtggatg ggcgatcaga tattttttcg ctcgccgtgg
      901 tgatgttcga actcctgacc ggtcgcctac cctttaccgc agacaacgtc tcggcactgc
      961 tgtttgccat tgctcacacg ccgcatctca gcgtgaaagc catcaggccg gacctccctc
     1021 ctaccataca ggaggtcctg gatcgagcgt tgcagaaaga cccggtccac cgttatcgcc
     1081 gggccggcga atttgccgcg gagctccgct cctgcctcca agggctggcc gcataataca
     1141 agctgatgct gcttctctga ttctccacgg gagtttcggt ttcttgccga cgcttcgcga
     1201 tcatcgttat aaatcgcgta acgctggcag tagaaag
//
LOCUS       DASTWE010000175         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_295505, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000175 DASTWE010000000
VERSION     DASTWE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295505"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFT30_01960"
     CDS             complement(<1..265)
                     /locus_tag="VFT30_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5091419.1"
                     /translation="MKVRIGRDELLTGLQRVQGVVEKRNTMPILSNILLEAKQDGVEI
                     VATDLEIGMRGLYKGTVLSTGGVTISARKLFEIVKELPPGDIDL"
     gene            complement(289..>1425)
                     /gene="dnaA"
                     /locus_tag="VFT30_01965"
     CDS             complement(289..>1425)
                     /gene="dnaA"
                     /locus_tag="VFT30_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5091420.1"
                     /translation="GGGLMAVAFTVREKGVSPQGEPQAVMNGPRGATQSKPRRGIQLN
                     PKYTFKNFVVGAGNQFAHAACMAVAEQPGHTYNPLFIYGGVGLGKTHLLNAIGNHVAE
                     KSDLRIAYLTTEQFTNEVINSIRYDKMMDLRKRYRHIDMLMIDDIQFLVGKERTQEEF
                     FHTFNALYEGHKQIVLSSDRFPKDMPDIEERLRSRFEWGLIADLQPPDVETRISILRK
                     KSEDEGVKLPEDVIQFLSITMKSNIRELEGSLVRLGAYASLTGQVITLDLAKNVLRDL
                     IGDKKKIVAMDDIQEAVCAQFHVKMTELKSRRRSKTLVHPRQIAMYLCRELTDASYPE
                     IGRQFGGKDHTTIIHACRQVTKAKETDTVLQTTIETLKEHILRN"
BASE COUNT          305 a          396 c          351 g          373 t
ORIGIN      
        1 tcaggtcgat gtcgccgggg ggcaactctt tgacgatttc gaatagtttt cgggctgaga
       61 tggttacacc gccggtcgaa agaacggtcc ctttgtacaa acctcgcatg ccgatctcaa
      121 ggtccgtcgc cacgatctcc accccatcct gcttggcttc caacagaatg ttcgacagaa
      181 tcggcatcgt gttccgtttc tccacgacac cttggacccg ctggagtcct gtcaacaatt
      241 catcccgccc aatacgtacc ttcatggaga actctcccgg tcaagccttt agtttcgaag
      301 aatgtgctcc ttcaatgttt caattgtcgt ctgcaacact gtgtcggtct ccttggcctt
      361 cgtgacctgg cgacaagcgt gaatgatggt cgtatggtcc ttgccgccga attggcgccc
      421 gatttcagga tatgatgcgt cggtcagctc gcgacacaga tacatggcga tctgtcgagg
      481 atgtacgagg gtcttgcttc gacggcggga tttcagttcc gtcatcttca catggaactg
      541 tgcacaaacc gcctcttgaa tatcatccat cgccacgatc tttttcttgt ccccgatgag
      601 atcgcggagg acattcttgg caagatcaag ggttatcact tgcccggtca atgaagcata
      661 ggcgcctaat cgaaccagac taccttccag ttcgcggata ttgctcttca ttgtgatcga
      721 caggaactgg atgacgtctt caggaagttt gactccctca tcctccgact tttttctcag
      781 gatggagata cgagtctcca catcgggcgg ctgtaagtcc gcaatcagcc cccattcgaa
      841 gcgagatcgt agacgctctt cgatatccgg catatctttg ggaaaccgat cgcttgagag
      901 gacaatttgt ttgtgtcctt cgtacaaggc attgaatgta tggaagaact cttcctgagt
      961 ccgttctttc ccaaccagga actgaatatc atcgatcatg agcatatcga tatgtcgata
     1021 acgcttccgc agatccatca tcttgtcata gcgaatggag ttgatgacct cgttggtaaa
     1081 ttgttccgtg gtcaggtacg cgatgcgcaa atcgctcttc tcagccacgt ggttccctat
     1141 ggcgtttaac aagtgagtct ttcccagtcc cacaccgccg taaataaaga gcgggttata
     1201 cgtgtgcccg ggctgctcgg ccactgccat gcaggccgcg tgggcaaatt gattcccagc
     1261 accaacgaca aagttcttga atgtatactt tgggttcagc tggattcccc gtcgaggctt
     1321 ggactgcgtt gcgccgcgag gtccattcat gacggcttgg ggttcaccct gcggcgaaac
     1381 tcctttctca cggacggtaa atgcgaccgc catcagcccg ccacc
//
LOCUS       DASTWE010000176         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_4920, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000176 DASTWE010000000
VERSION     DASTWE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4920"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..>1090
                     /locus_tag="VFT30_01970"
     CDS             191..>1090
                     /locus_tag="VFT30_01970"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEU5091421.1"
                     /translation="MKRRKLTKSIPVRACMVLGLLLGFSFGEVQAEEYKLKIPFGLEE
                     TAVVIPADNPLTKEKVELGRLLFFDKRLSQDNTIACANCHIAKFAFTDGKPVSTGIRG
                     QKGGRSAPASFNRVFSSAQFWDGRAATLEAQSVGPFTNPIEHGFANYDVMNAKMMKIP
                     GYRKLFKQVFGDDKITTERVGMAIASFQRTVLSGNSPADRFDQGGESGAIPEGAQKGL
                     ILFREKARCTKCHSGFNFTDEKFHNLGIGWDTNTVDLGRYMITNNPEEIGAFKTPTLR
                     EIARSAPYMHDGRFKTLEEVVNFY"
BASE COUNT          273 a          259 c          331 g          227 t
ORIGIN      
        1 gacccagcgt atgcaggtgc acgagaagac ggcgtggatg ctccgcagcc tgctggaata
       61 acgtgagagc gatgacagtt tccacgcacg gtcgaattga gaaggatcca gaggcaatga
      121 acgatgaagc ggtgagttcg cagaagggag tcaaaagtcc agatatagcc tgtagaagga
      181 ggagatgaag atgaagaggc ggaagttgac gaagtcgata cctgtgcgtg cgtgtatggt
      241 gttgggattg ctgttgggat tctctttcgg tgaagtacaa gcggaggagt acaagctcaa
      301 gattcctttt ggtctcgagg agactgctgt ggtcattccg gccgataatc ctttgacgaa
      361 ggagaaggtc gagttaggcc gattgctgtt ttttgacaag cggctgtcgc aggacaacac
      421 tatcgcctgc gcgaattgtc atatagcgaa attcgcgttc acggatggca agccggtctc
      481 caccggcatc cgaggccaga agggtggtcg cagcgcacca gcgtccttca accgggtgtt
      541 cagcagcgca caattttggg acggccgggc cgcgacattg gaagcccaat cggtcggtcc
      601 atttacgaat ccgatcgagc acggctttgc caactacgat gtgatgaatg cgaagatgat
      661 gaaaattcca ggctaccgga aactcttcaa gcaagtcttt ggtgatgaca agatcacgac
      721 cgagagggtg ggcatggcta tcgccagctt ccagcgtact gttctttccg gcaacagccc
      781 ggcagaccgg ttcgatcagg gaggggaatc aggagccatt ccggaaggcg ctcagaaggg
      841 cctgatcttg ttccgggaaa aggcgcgttg cacaaaatgc cactccgggt tcaatttcac
      901 cgacgaaaag tttcacaacc tcggcatcgg ctgggatacg aatacggtcg atctcggtcg
      961 gtatatgata acgaataacc cagaggagat tggagccttc aagaccccga ccttgcggga
     1021 gatcgctcga agtgctccgt acatgcacga cgggcgcttc aagacccttg aagaagtcgt
     1081 caatttctac
//
LOCUS       DASTWE010000177         1290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_295656, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000177 DASTWE010000000
VERSION     DASTWE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295656"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFT30_01975"
     CDS             <1..719
                     /locus_tag="VFT30_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU5091422.1"
                     /translation="GMIWLYLADERGHIDPLPPAPRMPLPSEPRQSFHISLTYTCTPD
                     DGIIGLIDPVHGPYVHSWWRSDARMHEKTKIFEPIPNGFRMTAHRPAKNSGPFHWMER
                     IFGGPLTTTIDFFLPNQRVELMQCGRAWLANRLMATPMSDMECRIDFSAYWVGLHWLP
                     FGNLIFRTLTKMFLGQDERAMKHLAVGLRHKPSSMFIGDADMPAKWYYKLKAAHQASV
                     QTGRPFDHPLKERVTLRWRS"
     gene            707..>1290
                     /locus_tag="VFT30_01980"
     CDS             707..>1290
                     /locus_tag="VFT30_01980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEU5091423.1"
                     /translation="MAKLTSPFRLACMLILAVMPGMLSHAGTSTIVSVPTLGVLGEGL
                     PGVVNYILVQLDHVAARDGPTVQFNEINLGGGSVVGEEWKEGARRAVRAVTHTVGDSG
                     HDWLITIKNRSVTSLTDGMSAGAVVAVAIMAAYRGDVIRSDVALSGQITPDGRLDVVG
                     GLPVKIEAAANAHYRTIIVSRDQPLTPDWMGATDV"
BASE COUNT          259 a          353 c          385 g          293 t
ORIGIN      
        1 acggcatgat ttggctgtat ctggccgacg aacgaggcca tatcgatccg ttgccgccag
       61 cgccgcgcat gccgcttcca tcggaacctc ggcaatcgtt ccatatttcg ctgacctata
      121 cctgcacgcc ggacgatggc atcatcggtc tcattgatcc ggttcatgga ccctatgttc
      181 attcttggtg gcgcagtgat gcgcgtatgc atgagaagac gaagatcttt gaaccgatcc
      241 cgaacggttt ccggatgact gctcaccgac cggcgaagaa cagcggcccg tttcattgga
      301 tggaacgtat cttcggaggg ccgctcacca cgacgatcga cttcttcctg ccgaatcaac
      361 gggtggaact catgcagtgc ggaagagcgt ggctcgcgaa tcggttgatg gctacgccga
      421 tgtccgatat ggaatgccgc atcgatttct ctgcgtattg ggtcgggctg cattggcttc
      481 cgttcggcaa tttgattttt cgtacgctga ccaaaatgtt tctcggccag gatgagcggg
      541 cgatgaaaca cttggcggtg ggacttcgcc acaaaccctc gtcgatgttt atcggcgatg
      601 ccgatatgcc ggcgaaatgg tactacaaac tgaaggccgc ccatcaggca tcggtccaaa
      661 caggccggcc ttttgatcat cctctcaagg aacgagtgac cctccgatgg cgaagttgac
      721 ctcgcccttt cggctggcat gcatgctgat attggcggtc atgcctggga tgctatcgca
      781 cgccgggacc tccacaatcg tctcagtccc gacgttgggc gtactgggcg agggccttcc
      841 cggtgtcgtg aactacatct tagtccagct cgatcacgtg gctgcgcgtg acggccccac
      901 tgttcagttc aatgaaatca acctcggagg agggtctgtc gtcggcgaag aatggaagga
      961 gggagcccgt cgagccgtgc gtgccgtgac ccacactgtg ggtgacagcg gtcatgattg
     1021 gttgattacg atcaaaaatc gctctgtgac gtctttgacc gatgggatga gtgccggtgc
     1081 cgttgtcgca gtggcgatta tggcagccta ccgaggagat gtgatcagat cggacgtggc
     1141 cctatccggc cagatcactc cagatggtcg tttggacgtg gtaggagggt tacccgtgaa
     1201 aatcgaggcc gccgcgaacg cgcattaccg aacaatcatc gtctctcggg atcagccact
     1261 cacgccggat tggatgggtg cgacggatgt
//
LOCUS       DASTWE010000178         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_263366, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000178 DASTWE010000000
VERSION     DASTWE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263366"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFT30_01985"
     CDS             complement(<1..1005)
                     /locus_tag="VFT30_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5091424.1"
                     /translation="MSDPSDGDTLESLRMFTGFSCTVRLGNESDIAETLDRLYGSNGG
                     AGKVLEETAEGTGDYAEAGDDIEHLRDMASEVPVIRLVNNLISRAVERRASDIHIEPF
                     EKEFRIRYRIDGILQTVESPPINLKPAIISRVKLMAKLNIAERRLPQDGRIKLKVLGK
                     EIDLRVSTLPTLYGESVVMRILDKSDSNAFDLRRLGFPEKLLAKIEKLTSMPHGILLV
                     TGPTGSGKSTTLYSAMKRINLPDKKIITIEDPVEYQMDGINQIHVNAQIGLTFASGLR
                     HIVRQDPDVIMIGEIRDLETAEIAIRSALTGHLVFSTLHTNDAPSAITRLVDMGVENY
                     L"
BASE COUNT          220 a          287 c          264 g          241 t
ORIGIN      
        1 aagatagttc tccactccca tgtccaccag ccgggtgatg gcgcttggag catcattggt
       61 atgaagagtg ctaaatacaa ggtgccccgt cagggcagac cggatcgcaa tctcagccgt
      121 ctcaagatcg cgaatctccc caatcatgat gacgtccggg tcctggcgaa caatgtggcg
      181 gagtcccgaa gcaaatgtca gaccaatctg agcgttgacg tggatttggt taatgccatc
      241 catctggtat tccaccggat cctcaattgt aatgatcttc ttgtccggca gattgatccg
      301 cttcattgca ctgtatagcg tcgtcgactt tccgctgccg gtcggcccgg tgacgagcag
      361 gatgccatgc ggcatcgagg tcaacttttc gatcttggca aggagttttt cggggaatcc
      421 gaggcggcgt agatcgaacg cattggagtc gctcttgtcc aggatgcgca tcacgacgct
      481 ctcaccatac aaagtaggca acgtcgaaac acgaagatcg atctctttgc ccaggacctt
      541 caacttgatg cgaccgtcct gaggcaagcg ccgttccgca atgttgagtt ttgccatcaa
      601 tttcactcga gagatgatgg caggcttaag attgatcggt ggcgactcga cggtctgcag
      661 aatgccgtcg attcgatagc gaatccgaaa ttccttctcg aacggctcga tatgaatgtc
      721 gctggctctc cgctccacgg cgcgcgagat gaggttgtta acaagtcgaa tgactggcac
      781 ctccgacgcc atgtcgcgca gatgctcgat atcgtcaccc gcctcagcgt agtcacccgt
      841 tccctcagcg gtttcttcca aaaccttccc tgcaccgcca ttgcttccgt agagccgatc
      901 taatgtctcg gcgatatcgc tttcgtttcc cagacgaacg gtgcagctaa atcccgtgaa
      961 catgcgcaag gactccagtg tgtctccgtc tgatgggtcc gacatagcca cg
//
LOCUS       DASTWE010000179         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_37229, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000179 DASTWE010000000
VERSION     DASTWE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37229"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFT30_01990"
     CDS             <1..906
                     /locus_tag="VFT30_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091425.1"
                     /translation="QATPVARTTAKFISFIEGRWISVVGILGLVAFLFRIYSRAPREA
                     ASVAAILIVLLTYPSTQFSLRHFFHLEVFFWIGLASLAMIPFDFPKLRACASGFSLWL
                     IGFLICGAISYTALVMIQDHMLKRETTALLMSPSQSIATTVIDWPGNRSLFRVPVPSQ
                     YEPLVRSAPDSLHLPSMYTTSPFEVRAAADRLAIVLGGPECATGPITIDIVYDTTAWT
                     WQPFDRSLSIQIGEQGMGDTVVIVPAIYRPTQSFAGVAVPGKLSGCVRAVNRIEDAKR
                     LPTIFSAVLGPNWKNEPFFLNLGKF"
     gene            1000..1674
                     /locus_tag="VFT30_01995"
     CDS             1000..1674
                     /locus_tag="VFT30_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5091426.1"
                     /translation="MAASLYTVVIPVFNGATIIARTIDRVDWYFRTRGLAFELIVVND
                     GSSDGTWDVLKREAGQRSNLIALNLVKNYGQHAATQCGLRVATGDYVITMDDDLQHDP
                     IDIDKLIAKAAQGHDVVFARFERARRAWHRRPETVLATALVRLVYKIPSDIKISSFRL
                     LRRDVVNRMLVLETSKPYVTGLALHAAKNPGNADVCTREGIPTNPPDQTDLRAAYFRV
                     SERLST"
     gene            complement(1641..2345)
                     /locus_tag="VFT30_02000"
     CDS             complement(1641..2345)
                     /locus_tag="VFT30_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU5091427.1"
                     /translation="MIGPEKIPRLRRQLLVILGGIAGGAIVSETIKRCAAAGAAIELL
                     GYLNDAMSPGTNLAGHPVLGRFEQRRECAAEAKFISAIMKPREARTRFARIRSLDIPY
                     DRWATVMDPTAIVTSDASVGCGSFVGAGSIVESGAVVGNHCHLRANCYLSHDVKLGDF
                     VSVGPHATVLGRSMIGEGAHFGANSVCRDGILIGRYALVGVGAVVTGDVPDFTIVAGN
                     PARSIGQVDKRSDTLK"
BASE COUNT          583 a          803 c          735 g          605 t
ORIGIN      
        1 caggcaactc ccgtagcgcg aaccaccgcc aagttcatct cctttatcga gggtcgttgg
       61 atatcggtcg ttgggatcct cggccttgtt gcgttcttgt ttcgaatcta ttcgcgggct
      121 ccgcgcgaag cggcaagcgt agccgcgata ctgatcgtgc tcctgacata tccgagcacg
      181 caattctctt tacgacattt ttttcatctg gaggtgtttt tttggatcgg ccttgcttcg
      241 ctggccatga tccctttcga ttttccaaag cttcgggctt gcgcatcagg tttttctcta
      301 tggctcatcg gcttcctgat ctgtggagcg atttcgtaca ccgcgctggt tatgattcag
      361 gatcatatgc tgaagagaga aacgacggcc ctattgatga gcccgagcca atcgatcgca
      421 accaccgtca tcgactggcc cggcaatcgg tcactcttca gggttccagt accgtctcaa
      481 tatgaacccc ttgttcggag tgcaccggac agtctgcacc tgccgtcgat gtatacgacc
      541 tctccttttg aggtgcgcgc tgcggcggat cgtctggcca tcgtactcgg tgggccagag
      601 tgcgctaccg gaccaattac gatcgatatc gtgtacgaca cgacagcttg gacgtggcag
      661 ccgttcgaca gaagtctttc aatccaaatt ggggagcaag ggatgggcga caccgttgtg
      721 atcgtcccgg cgatttaccg cccgacgcag tctttcgctg gagtggccgt tcccggcaag
      781 ctgtcagggt gcgttcgcgc agtaaatcga atcgaggatg cgaaacgctt gccgacaatt
      841 ttttcagcag ttctcggccc gaactggaag aatgaaccat tcttcctgaa cctcggaaag
      901 ttttgaaccc ttgcgggtac cgcctccgtg ttatagtagc gtacgaaaat gtataacttc
      961 gccgtttgac agcgcggcga atacaccgtc ggggcggcga tggctgcgag cctgtacact
     1021 gttgtgatcc cagtgttcaa cggcgccacg atcatcgccc ggacaatcga ccgagtggat
     1081 tggtatttcc gaacgcgtgg cctcgctttt gaactcattg tcgtgaatga cggcagctca
     1141 gacggcacgt gggacgttct caaacgcgag gccggacaac gctcaaatct tatcgccctt
     1201 aatttggtca agaactacgg ccagcacgcg gcaacccaat gtggcttgcg tgttgccacg
     1261 ggagactacg tcatcaccat ggatgacgat ctgcagcatg atccgatcga tatcgacaaa
     1321 ctcatcgcga aggctgcgca gggacatgat gtcgtttttg cacgatttga gcgggcgcgg
     1381 cgggcctggc atcgccgtcc ggaaacggtc ttggcaacag cgttggttcg actcgtgtat
     1441 aaaattccgt cggacatcaa gatatcgagc ttccgactac tgcgacggga cgtggtgaac
     1501 cgcatgctgg ttctcgagac aagcaagcct tacgtgacgg gccttgcctt gcacgcggcg
     1561 aagaatcctg ggaacgccga cgtttgcact cgcgaaggca tacccacaaa ccctccagat
     1621 cagactgatc tgcgcgccgc ttacttcagg gtgtcggagc gcttgtcaac ttgacctatt
     1681 gaacgggcgg gatttccggc gacgatcgtg aagtccggaa cgtcgccagt caccacagcc
     1741 ccgacaccta cgagcgcata gcgaccaatc aagatcccat ctcgacagac tgaatttgcg
     1801 ccgaaatgtg cgccttcccc gatcatggag cggcccaaga cggtggcatg cggccccaca
     1861 gaaacgaaat cgccgagctt cacatcgtgg ctcaaatagc aatttgcacg aagatggcag
     1921 tggttgccga ccacagcgcc cgactcgacg attgacccag cgccaacgaa actcccgcat
     1981 cctacggatg cgtccgaagt tactatagcc gtcgggtcca tcacggtggc ccaccgatca
     2041 tagggaatgt ccagcgacct gatacgcgcg aaccgggtcc gagcctcacg aggtttcatg
     2101 atcgcgctga tgaatttggc ctcagcagca cattcccgcc gttgctcaaa ccgtcccaat
     2161 acaggatgcc ccgccaagtt ggttcctggg ctcattgcat cgttcaagta gccaagcagc
     2221 tcgatagctg ctcctgctgc cgcgcatctc ttgatcgttt cgcttacgat ggctccaccg
     2281 gctatcccac ccaggatcac gagaagctgg cgcctcaatc gagggatctt ttcgggacca
     2341 atcaccgggg gagcctgcca aacggattgt cgtgaccagg taaccaatgc ctccgataac
     2401 acgtaagcga tcaggccgcc cggccgagcg gtcactaact tccgaaggcc gtcatatatt
     2461 ccaaacgggc acgtcagcgc cacactaatt attgccccgt cacgtccatg ccgtatctac
     2521 tcacgtcgga aattggcact cagcggaagg caacgcactc tgatccatgt ccgcttttgg
     2581 gagtaaagct gacatcccgg acatggccac tatgtcgcct aatgacgcac ttaacggaca
     2641 tccgacgggt cagccccaat gtccgcttcc ggatgaatag cggacgggca cgactgatat
     2701 tggttggagt ccggtactct cgctgc
//
LOCUS       DASTWE010000180         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_489613, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000180 DASTWE010000000
VERSION     DASTWE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489613"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..922
                     /locus_tag="VFT30_02005"
     CDS             377..922
                     /locus_tag="VFT30_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5091428.1"
                     /translation="MDRDGKSVRFVTLVEPHLADAYALARWLTRNRDDADEVLQEACI
                     RALAAIEQQSGTNVRAWLLAIIRNTAYTWLKAKKRISLIGLDSLSEKDLARVEEGGAQ
                     GVPTTDPEAEMIARADAQNLEKHINELPIEFREALVLRDIQGLGYHEIAQVTGVPVGT
                     VMSRLARARRRLISALKETEA"
     gene            922..1671
                     /locus_tag="VFT30_02010"
     CDS             922..1671
                     /locus_tag="VFT30_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU5091429.1"
                     /translation="MALATESDQDGARLLVHAYLDGELDVANGLAVKQKIDTDSNLAA
                     EAADIQELKALLRGKFPSEPLPSHLKQKIDSLHRQVGRTRRPAWGSMAAAILVAIALS
                     NASTWFVARIMTSSTQTSELVDSHLRSLIAPQPTDVASSDRHTVKPWFNGKVAQSPRV
                     KDLSEAGFPLLGGRVDVLQKVAVPTLVYRRRLHVISVTSAPTAVSGTADDVINGFNIV
                     RWDEEGMSYWAISDLNPAELNEFARLYRQRS"
BASE COUNT          421 a          460 c          525 g          426 t
ORIGIN      
        1 gatgcgagtt caactctttc ggtcccatca cgaaccgggt cggtgaggct gtgaagccca
       61 agaagtactg cgatcgcgac aattgaatag agaacgacga tgaaaccgtc ctgggacttt
      121 gaggctattc ggctttcaat gcgctcgtaa gtaatggtgg acatccgcct tctcctcgat
      181 tgagacccgc gctgcctaaa aacgatgcgt tcccccggca cgtcttgaaa cctggtccta
      241 ctgaccggaa aatattgacc ggtccttgcc ggggtgactc tcgtgggttg cgcaaaattc
      301 ccatgatggg tattttttta ttttgctggg gatggggaat tttttatcgg ttttcagagt
      361 caactaaaaa gactgcatgg atcgcgacgg aaagagcgtc aggtttgtta ccttggtcga
      421 gcctcatctc gccgatgcat atgcacttgc tcgatggcta acccgcaata gggatgatgc
      481 tgacgaggta ttgcaggagg catgcattcg tgcactggca gccatagagc aacaatcggg
      541 cacgaatgta cgtgcatggc ttttggcgat aatccgtaat acggcatata cttggctgaa
      601 agcgaagaag agaataagct tgattggctt ggactcgctt tcggaaaagg acttggcccg
      661 ggtggaagag ggcggcgcgc agggcgtgcc aacgacggac ccggaagcgg agatgatagc
      721 tcgtgctgat gcgcaaaatc tggaaaagca tatcaatgag ctaccaatcg agtttcgaga
      781 ggctctggtg ctgcgcgaca ttcagggatt aggatatcac gagatcgccc aggtgacggg
      841 cgttccagtc ggtaccgtta tgtcaagact ggcgcgtgct cgaagacgtt taatctcagc
      901 gctgaaggaa accgaagctt aatggcgcta gcaaccgaaa gtgatcaaga tggcgctcgt
      961 ttgcttgtcc atgcctatct ggacggcgaa ctcgacgttg caaacggttt ggccgtcaaa
     1021 cagaagatcg atacggattc caaccttgca gctgaagccg cagacatcca ggagctcaag
     1081 gcgctgctgc ggggcaagtt tcccagcgaa cctttgccct ctcacttgaa gcagaaaatc
     1141 gatagccttc accgtcaggt cggtcgcact cgtcggccag cgtggggatc gatggcggca
     1201 gcgatcttgg tggctattgc gctatccaat gcgtcaacct ggtttgtcgc gcgcattatg
     1261 acatccagta cgcaaacgtc agaattggtc gatagtcatc tacgctcctt gattgcgccg
     1321 cagccaaccg atgttgcatc ttctgacagg cataccgtca aaccttggtt taatggcaag
     1381 gttgcccagt ccccgcgagt taaggatctg tccgaagcag ggttcccctt attgggtggg
     1441 cgcgtcgatg ttctgcagaa agtcgccgtg ccgaccttgg tataccgcag gcggctccat
     1501 gtcatcagtg tgacatctgc cccaactgcc gtgagcggta cagccgacga tgtcatcaat
     1561 ggattcaaca tcgttcgctg ggatgaggag gggatgtcct actgggcgat ctccgacttg
     1621 aacccggcag aactcaatga gtttgccagg ctttatcgtc agcgttcgta gttgtttctg
     1681 gctcgtaatc cagcttaaac acggatggcg cagcttatcc gaacgacggc gcgagcgccc
     1741 gatggccgga agctatctac tctctctcca aagcacatga ggagggagtt ctggttgcga
     1801 cgaatctaaa tcaattgctc ggcggtctag ag
//
LOCUS       DASTWE010000181         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_424875, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000181 DASTWE010000000
VERSION     DASTWE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424875"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFT30_02015"
     CDS             <1..704
                     /locus_tag="VFT30_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091430.1"
                     /translation="GYRQRVAADIAALPSPPLNAALFSRDAIEVLGSGVAVDGNDQST
                     LSPAPLNGLESASTIGGNLTSVTGSPLPDRPLSSYSYDMRGLLKTLKPPFTKKIEQIV
                     PGITILADGTYVGNGLSLGQIPTSGDTSQSTYVDGPLNISDSSGQGILVINGDLSVTG
                     NFTYFGLIIVKGKIHLNGGGMEGVKIYGAMIASSDAGIGQSVLEGIVQIRNDSAMIQA
                     QFNRLQYARLVFREM"
     gene            complement(820..>1001)
                     /locus_tag="VFT30_02020"
     CDS             complement(820..>1001)
                     /locus_tag="VFT30_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091431.1"
                     /translation="NGCGISSEDLQRIFVPFFTSKSSGSGLGLPIVQKIVLMHDGKVE
                     VESKPGGGTCVNVYL"
BASE COUNT          249 a          274 c          251 g          227 t
ORIGIN      
        1 gcggttacag acagcgcgtg gcagccgata ttgcagcgct tccctctccg cctttgaatg
       61 ctgctctctt ttcaagggac gccatcgagg tgttgggatc aggagtggcc gtggacggaa
      121 atgaccagag cactctcagc ccggccccac tcaatggtct tgagtcggca agcacgattg
      181 gcggcaactt gacgagtgtc actgggtcgc ctctccctga caggccgctc tccagttact
      241 cctacgacat gaggggtctc ctcaagactt tgaagccacc gtttacaaag aaaatcgaac
      301 aaatcgttcc tggcattaca atccttgcgg atggaaccta tgtgggaaac ggcctcagcc
      361 ttggacagat accgaccagt ggcgacactt cgcaatcgac ctacgtcgac ggaccactga
      421 acatctcaga ctccagcggc caaggcattc ttgtgatcaa cggagatctt tcagtaacgg
      481 gaaattttac ctatttcgga ctgatcatcg tcaaagggaa gattcatctg aatggcggtg
      541 ggatggaagg cgttaaaatt tatggggcga tgattgcaag ctccgacgca ggtatcggtc
      601 aatccgttct tgaaggtatc gtgcaaattc gaaacgattc cgccatgatc caggcgcaat
      661 tcaaccgcct gcaatacgct cggctggtgt ttcgcgaaat gtgatcgcag cttgccagat
      721 tgcttcccta gctaaagtgc aagacggcca tgaaaggatc ctgcatcctc gttctcggct
      781 cttcttcaag aacgagcgaa ggacgacgac taccgactgt cacaaataga cattcacaca
      841 ggttccccct ccaggttttg actcgacttc gacctttccg tcgtgcatga gcacgatctt
      901 ctgcacaatc ggcaagccca aaccagagcc actgctcttg ctggtaaaaa atggaacaaa
      961 gatgcgctgc aagtcttcag aggaaatgcc acaaccattg t
//
LOCUS       DASTWE010000182         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_392774, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000182 DASTWE010000000
VERSION     DASTWE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392774"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="lpxK"
                     /locus_tag="VFT30_02025"
     CDS             complement(<1..1028)
                     /gene="lpxK"
                     /locus_tag="VFT30_02025"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU5091432.1"
                     /translation="MYGLIIRFRLWCYRKGWLTTTRLPCRVVSVGNLTVGGTGKTPIV
                     ILLTEWLLAKGHRVAILSRGYKRTSKARRLLVSDGSRILVGPLEAGDEPFLMAQRCPR
                     AIVAVGADRVALGQWVLEQYPVDCIVLDDGFQHRSLHRDVDLVLLDATDAAGLDALLP
                     AGRLREPLTELYRANAVMITRADSRQDVDAIRSRLQVISCSSDDAVEVVFRPESVVAI
                     VSGERQPVEWCRMKKTWLVSGIGNSRSFRRSAESMGVEILGETAFDDHHGYEHDDMEK
                     IRDNLQATGCEIVLTTEKDGGKLLPFLMPSDPWWMLHLGTEVMRGEERLYRLIGGPLI
                     GKPEQVGSR"
BASE COUNT          259 a          332 c          242 g          224 t
ORIGIN      
        1 cgagatccca cctgttctgg cttaccgatt aacggtccac cgatcaacct gtacaaccgc
       61 tcttctccgc gcatgacttc cgtcccaaga tgaagcatcc accaaggatc gcttggcatg
      121 aggaagggaa gcagcttccc accgtctttc tcagtcgtca gaacaatctc gcaaccagtg
      181 gcttgtagat tgtcacgaat cttctccata tcgtcgtgtt catagccatg atgatcgtca
      241 aaggcagtct cacctaagat ctcaaccccc atggattcgg ctgatcggcg gaatgatcgg
      301 ctattcccga tgccgctgac caaccaagtt tttttcattc gacaccattc aaccggttgc
      361 cgctcacccg aaacgatcgc cacgacggac tccggtcgaa acacaacttc aacggcgtcg
      421 tcggatgaac atgaaatcac ttgcaatcgg ctacgaattg cgtccacatc ctgacgagaa
      481 tcggcacggg tgatcatgac cgcattcgct cgatacagtt cggtcaaggg ttctcgtagt
      541 cttccggccg gaagcagcgc gtcaagtcct gcggcatcgg tagcatccaa cagcacaaga
      601 tcgacatcac gatgaagcga ccgatgttga aagccatcat ccaaaacgat gcagtcgact
      661 ggatactgtt ccaagaccca ttgccctaac gcaacccgat cggctccaac cgcgacaatc
      721 gcccgtggac aacgctgtgc catgagaaac ggctcgtccc ctgcttctaa cggtccaacc
      781 aaaattcgtg atccatcgga cacgagaaga cgacgtgcct tgctcgtccg tttgtacccc
      841 cggctcaaga tcgccaccct gtgccctttg gccaacagcc attccgttag gagaatgacg
      901 atgggggttt ttcctgtccc cccgaccgta agattcccca cgctcacgac acgacagggc
      961 agtcgtgttg tcgtaagcca acctttccga tagcaccaca gtcgaaatcg gatgatgagg
     1021 ccgtacaacc tagcaaccca gtaaagccct ttacagg
//
LOCUS       DASTWE010000183         1462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_942188, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000183 DASTWE010000000
VERSION     DASTWE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942188"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..928
                     /gene="phoU"
                     /locus_tag="VFT30_02030"
     CDS             230..928
                     /gene="phoU"
                     /locus_tag="VFT30_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5091433.1"
                     /translation="MQRNTFSQELHEVKDEILLLSSMVEQAVMDSVTALKDNDLEGAR
                     QILSNDLFINRKRFEIETSIMVLIATQQPIAHDLRLLASSLDICTELERMGDYAKGIA
                     NINLRSEGLSLPHIRKDIYLMAEKAVDMLHRAMTAFADEDTVTAGDIIQEDVIIDECY
                     SRLYQDAVQSVLRDPRNMERANYVIWAAHNLERLGDRVTNICERVIYIVRAERPRWKA
                     VPRDSKLFVPEYKW"
     gene            922..>1462
                     /locus_tag="VFT30_02035"
     CDS             922..>1462
                     /locus_tag="VFT30_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091434.1"
                     /translation="MVNISAIDVGSNAMRMVVGEVDEAWQVRPIENIRLPVRLGQDVF
                     DRGYLEEKTIQQTEEVFLRFKQMAENFNVHQLRAVATSAAREAANSDLLVDRVFRTSG
                     IEIEIISGEEEARLIHAAVAHVLNLKEKRTLLIDIGGGSVEVTLSTGRNIISTDSYDM
                     GTVRLLKKLNGNKSKHPLGK"
BASE COUNT          366 a          353 c          400 g          343 t
ORIGIN      
        1 attatgaaaa ctcgaatcgg ctgtcgccgt tctcgattct gagttttacc aatcgttaac
       61 aatcctttcc cgccctgtta attgtcccat gctacattta cagcgtaccg cacgcaggtc
      121 ttatctgtat aaccagacga atctctactt ctagccctcg tgtaccgaga agaatttcgt
      181 cgcatcagac gagcggcaga aaaatcagca cacaaacttg gaattaatta tgcagcgtaa
      241 taccttcagt caggaattgc acgaagtcaa agatgagatc cttctgctca gcagcatggt
      301 ggaacaggcg gtcatggact ccgtaacggc actgaaagat aatgacctgg agggtgcgcg
      361 gcaaattctt tccaatgatc tcttcatcaa tcgaaaacgt tttgagatcg agacctctat
      421 tatggtcctc attgcaacgc agcagcctat cgctcacgac ctgcgccttc tggcttccag
      481 cctggatatc tgcaccgaat tggagcgcat gggagattat gctaagggca tcgccaatat
      541 caatctccgt tcagaaggtt tgagcctgcc tcatatccgc aaggacattt acttaatggc
      601 agagaaagcc gtggatatgc tgcaccgggc gatgacggcc tttgccgacg aggatacagt
      661 aacggcgggg gatatcatcc aggaagatgt tattattgat gagtgttatt ccaggttgta
      721 ccaggatgct gtccaaagcg tgttgcggga tcccagaaat atggagcgcg ccaactatgt
      781 catctgggca gctcacaatc tggaacggtt gggtgaccgt gttaccaata tctgtgagcg
      841 agttatttat atcgttcggg ccgagcgtcc ccggtggaag gcagtcccca gggactcgaa
      901 actattcgtg cctgagtaca aatggtgaat atttctgcca ttgacgttgg gtcgaatgcc
      961 atgcgcatgg tggtcggcga ggtggatgag gcctggcagg tgcgcccgat cgagaatatc
     1021 cgccttccgg tgcgcctcgg gcaggatgtg tttgacaggg gatatctgga agaaaagacc
     1081 attcaacaaa cagaggaagt atttttacgt ttcaagcaga tggctgagaa tttcaatgtt
     1141 catcaactgc gtgccgtcgc caccagcgcg gcgagggagg ccgcaaacag cgatctcctg
     1201 gtagaccgtg tgttccggac cagcgggatc gagatcgaga tcatcagtgg ggaagaggag
     1261 gcacgcctga tccatgcggc cgtcgcgcat gtgctgaatc tcaaggagaa gcgcacgttg
     1321 ttgatcgata tcggtggagg cagcgtggag gtcactctct ccacagggcg gaatatcatt
     1381 tccacggaca gctacgatat ggggacggtg cgtctcctca aaaaattgaa tggcaacaag
     1441 tccaaacatc cgctggggaa gc
//
LOCUS       DASTWE010000184         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_5214, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000184 DASTWE010000000
VERSION     DASTWE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5214"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFT30_02040"
     CDS             complement(<1..834)
                     /locus_tag="VFT30_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5091435.1"
                     /translation="MGLLTAWPAWGLTAGDLYRSGKEATALVIGIDDEAHALSFGSGV
                     FISQDGLLLTNAHVIHDSTRILVYGAAGGQIPTPAPEIVAMDPDLDLAALRLTYAHPV
                     PYLSLAKEPPEDGLAAVTIGYPRLPDVLQMGLTIHPTVFPVTVSGEAMGQSRIAHRPL
                     PFLHITGLMNAGSSGGPLVAAESGDILGLVVHTVPYMEAAKNSQGESIGRVMLRAGLS
                     YCIPSARIRDWLDANHLSKSESRELFSQTQQALPKPTASTGFVTTAHLLQLMADVIRK
                     DS"
     gene            complement(1181..1600)
                     /locus_tag="VFT30_02045"
     CDS             complement(1181..1600)
                     /locus_tag="VFT30_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEU5091436.1"
                     /translation="MAKTLIEPTSTSAEKLRTMLLDKRAAVQQQINELLAQYREHYST
                     THNDSVLDLEDRSLRDSTAEQQLSLLESRNHTRVMLDTALRRLDDGEYGLCEDCGADI
                     GPGRLSALPFAKRCLSCQHQAEVLEKIARQEDRRDDR"
BASE COUNT          371 a          452 c          462 g          376 t
ORIGIN      
        1 ggaatccttc cgaatgacgt ccgccatcag ctgcagtaga tgagccgtgg tgacgaatcc
       61 agtggacgcg gtgggcttcg gcagggcctg ttgtgtttga ctgaatagct cacgagactc
      121 gctcttcgag agatgattgg cgtcaagcca gtcgcgaatc ctcgctgatg gaatgcaata
      181 gcttaaacct gctcgtaaca tcacccgtcc gatcgactcg ccctgtgaat ttttggccgc
      241 ttccatataa ggaacagtat gcaccacgag tccgaggata tctccggact cggccgcgac
      301 gagcggaccg ccgctgcttc ccgcattcat gagccccgta atgtgaagaa agggcagcgg
      361 cctgtgggcg atgcgggact gccccatcgc ctctccgctc accgtcacgg ggaatactgt
      421 cggatggatc gttaacccca tctgaagcac atccggcaag cggggatatc caatcgtcac
      481 ggcggccagg ccgtcttccg gtggctcctt cgccaacgac aagtacggga cgggatgagc
      541 gtatgtgagg cgtagcgctg caagatcaag atcaggatcc atcgctacga tctcgggagc
      601 gggagtcggt atctggccgc cggccgctcc atagaccaag atacgcgtgg aatcatgaat
      661 gacatgtgcg ttggtaagca gcaaaccgtc ttgagagatg aacacgccgc tgccaaaaga
      721 gagagcgtgc gcttcatcat cgattccgat gacgagtgct gtcgcttctt tgccggaacg
      781 gtacagatct cctgccgtca acccccaggc aggccatgca gtgagtagac ccacgcagat
      841 caggaaggta cggatagcga cgaaactatg aagtcctgcg agtgtgatgg tgcccctcgg
      901 tacgacgggt gcactttaca ctaagcaggt atggtgaata aagccagtag attctcataa
      961 agagcatggg agcaacacta ggcgaacccc tggttacgat gaatcgttag gcagaaaagc
     1021 gctaccttga taagtacgct cttacgacgg ctttcatttg cttatcttgc ttatgccccg
     1081 aacttcttac cgtgtgagga ataaccatac ctcggccact gttctacgag tgcctttcct
     1141 acataaagtc tttgaaatgt agtgggtatg cgggcacggt ctacctgtca tcccggcgat
     1201 cttcttgccg cgcaattttt tccagcacct cggcctgatg ttggcacgac aggcaacgct
     1261 ttgcaaaggg gagggcgctc agacgccctg gacctatgtc ggcaccgcaa tcctcgcaca
     1321 atccatactc tccatcgtct agccggcgga gcgccgtatc cagcatcacg cgcgtgtggt
     1381 tgcgggattc cagcagggac aattgctgtt cggccgtgct gtcgcgcaac gacctgtctt
     1441 caagatcaag cacggaatca ttgtgcgtcg tggagtagtg ctcccgatat tgagccaaga
     1501 gctcattgat ctgctgctga actgccgccc gtttgtcgag cagcatcgtt cgaagcttct
     1561 ccgctgatgt ggaagtaggt tcaatgagtg tcttggccat gatcaagctc ctcatgttaa
     1621 gttttgaatc gtgacagtaa ttttgcttga tctgcaaaag t
//
LOCUS       DASTWE010000185         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1007016, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000185 DASTWE010000000
VERSION     DASTWE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007016"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..350)
                     /locus_tag="VFT30_02050"
     CDS             complement(81..350)
                     /locus_tag="VFT30_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091437.1"
                     /translation="MICIILGLAPLAVAQDVHHELAAADSDGHEHSDTDICQWVQHHI
                     AGSVDLDLPRLAVCDIARHQELLSESILRSAALSLVGPSRAPPQV"
     gene            584..1645
                     /locus_tag="VFT30_02055"
     CDS             584..1645
                     /locus_tag="VFT30_02055"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5091438.1"
                     /translation="MNRPIDNQHVIEIKALPSPRAIKTKLPITDQAAALVVETREAIR
                     RILHGKDRDRLLVIVGPCSIHDPEAAYEYATKLKPVADALRDRLLIVMRTYFEKPRTT
                     IGWKGLINDPHLDGTCDIAVGMELARSILLQINQLGLPCGTELLDPISPQYVADLISW
                     AAIGARTTESQTHREMASGVSMPVGFKNGTEGSLQVAVNAMISARTLHHFVGINADGQ
                     TAIIRTMGNPDRHIVLRGGGGRTNYEAEQVAKAEAAVAGEGIARPIMIDCSHDNSKKD
                     HTRQGLVAHEVLRQFREGRQTIMGLMLESNLNPGRQAWKEGVALLHGVSITDACLGWD
                     ETEHLLLELAASIAPKPVF"
BASE COUNT          480 a          520 c          517 g          413 t
ORIGIN      
        1 ttaaatgaga atggatgaaa taggtgtgct tggacctcac ggccctacca accatcctga
       61 ctgcttggcg tgatattcgg ttatacttga ggaggggcgc gggatgggcc gacaagcgag
      121 agagcggccg aacgcagaat ggattcagaa agaagttcct ggtgccgggc gatgtcgcaa
      181 acggctaatc gtggaagatc gagatcaacc gagccggcga tatggtgctg gacccactgg
      241 cagatgtcgg tgtctgaatg ttcgtgaccg tccgaatcgg ccgcagccag ttcatggtgg
      301 acatcctgag ccacggcgag cggagccagt cccaggatga tacagatcat tcccaatgcg
      361 actcctgcac ggagtaacga tgagagggat ctgaacgtca tcgaaaacat agcggtagaa
      421 taggcatcca tgatgagaat tgcaagaact gctgagatag cgcagcaatc agctcgattt
      481 accgaccgaa ttgaccggta tggacttttc cggcatgatt cgttacattg ctaacccctc
      541 acgatctcaa caagaatgag atctctgaag gacatgatcc ctcatgaata gaccgatcga
      601 caatcagcac gttatagaaa tcaaggcgtt gccatccccg cgcgccatta agaccaaact
      661 gcctattacc gatcaggctg cggcactcgt cgttgaaacc cgagaagcga ttcgccgaat
      721 tctccatgga aaggatcgtg acaggctcct ggtcattgta ggaccgtgct ccatccatga
      781 cccagaagct gcctatgagt acgccaccaa gctcaagccg gttgccgatg ctttgcgtga
      841 ccgtctcctg atcgtgatgc ggacttactt tgaaaaacca cgaaccacca tcggatggaa
      901 agggctcatt aatgaccctc acctcgatgg aacctgtgat atcgcggtgg ggatggagct
      961 ggcgcgatcg attcttcttc aaatcaatca attgggtctt ccctgcggga cggaactact
     1021 cgatccgatc agtccgcagt acgttgcaga tctcatcagc tgggcagcga tcggcgcccg
     1081 caccacggaa agccaaaccc atcgcgaaat ggccagcggc gtgtccatgc cggtcggctt
     1141 caagaatggc actgagggca gcttacaggt tgccgtcaat gccatgattt ccgcccgcac
     1201 actacatcat tttgtcggca tcaacgcaga cggccagact gcgatcatca gaactatggg
     1261 caaccccgac cgccatatcg ttctccgtgg tggaggagga aggacgaact acgaagcaga
     1321 acaggtggct aaagcagaag ccgcagtcgc cggagagggc attgcccgtc cgatcatgat
     1381 cgattgttcg cacgacaact ccaagaaaga ccacacgcgc caagggttgg tcgctcatga
     1441 agttttgcgg cagttccgtg aaggccgcca aaccatcatg ggtctcatgc tcgaaagcaa
     1501 cctcaatccc ggcagacagg cgtggaaaga aggtgtcgcc ctccttcatg gcgtctcaat
     1561 taccgatgca tgtctaggct gggatgagac cgaacatctg ctgcttgagc tcgccgcttc
     1621 gatcgctccc aagcccgtct tttaatccta cctttcatca tctaattccc agaaccaagg
     1681 tttctctatt ttctgattga tagccggctt tacgccacga ttgaagggaa cggtatggca
     1741 agcacactcc aataggaatc agtgccggca cggctctcct cttgatgcaa ttcagatgag
     1801 tcggcctagc gcaggcatca tctaaccgtt actaaagttc gaggttataa cggtccaatg
     1861 tcgtggcttt atgacgggcg tggttggaga gcgacttgtt gtaatcgacg atagcgcgta
     1921 actcgttccc
//
LOCUS       DASTWE010000186         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1523907, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000186 DASTWE010000000
VERSION     DASTWE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523907"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..>1002
                     /gene="amrB"
                     /locus_tag="VFT30_02060"
     CDS             219..>1002
                     /gene="amrB"
                     /locus_tag="VFT30_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEU5091439.1"
                     /translation="MTTVAAKDSDHYPVLRNLQFSPIKQGDDQLIVLWDPSGLSKEKL
                     VLPLNFFFIVQHFDGEHSLQEIGALYLKRFGEFLLPSKVEQLVVDLDQKLFLESLRAE
                     TARQQARIDYRQRPSRPAVFAGRSYEADSAKLRKQIDGFFTSGEGPDIKPSEHRGKLI
                     KGLVAPTYDLKQAGPVYAWAYKELQECQQPNVYILIGTAHAGLENLFAVTDKDFETPL
                     GVVPVDHPIVNRLKAVAPEFFEEDIAHQSEHAIEFQLPFLQTV"
BASE COUNT          229 a          266 c          268 g          239 t
ORIGIN      
        1 gatcgaattc tccgatgcat cgtatgggcg accgaccctc tggttacttg tcgtgactgt
       61 cgtcgcgctg cttgttctca gccgccgcgc gagactgaag cctccacaag aaggttgagg
      121 ttgaggataa ggttgagtag gaactcagcc tcagcctcga ccttagcatt ctgaccagtt
      181 gcggcttgtt ctgttcctcc aatacaatga ccaccgtcat gactaccgtt gccgcaaagg
      241 attccgacca ctatccggtc ttgcggaatc tccagttctc acccattaag cagggagacg
      301 atcagctgat cgtactctgg gacccgagcg ggctcagtaa agagaagctt gtcttgcctt
      361 tgaacttttt cttcattgtg caacatttcg atggcgaaca ttcgctacaa gagatcggtg
      421 cgttgtactt gaaacggttc ggcgagtttc tcttgccgag caaagtagag caactggtgg
      481 tggatctgga ccagaagctc ttcttggaaa gcctgcgagc tgagacggca cggcaacagg
      541 ctcggatcga ctatcggcaa cggccgtccc gcccggcggt ctttgccgga cggagctatg
      601 aagcggatag cgccaaacta cggaaacaga tcgatggttt tttcacatcg ggcgagggac
      661 cggatatcaa gccgtcggaa catcgtggca agttgatcaa aggcttagtt gcgccaacct
      721 atgatctcaa gcaagctggg ccggtctatg cctgggccta caaagaacta caagaatgcc
      781 aacaacctaa cgtctatatt ctgattggaa ccgcccatgc cggtcttgaa aatctctttg
      841 ccgtcacgga caaggatttt gagacaccgc tcggtgtggt gccggtggac catccgattg
      901 tgaatcgcct caaggctgtt gcaccggagt tcttcgagga ggacatcgct caccagtcag
      961 agcatgcgat cgaatttcaa ttgcccttcc ttcagactgt ta
//
LOCUS       DASTWE010000187         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1200736, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000187 DASTWE010000000
VERSION     DASTWE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200736"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..>1025)
                     /locus_tag="VFT30_02065"
     CDS             complement(124..>1025)
                     /locus_tag="VFT30_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU5091440.1"
                     /translation="ITDERMLQIAREMNLSETVFVLPPSEEGKAEPPPTAADEIGSKP
                     SVLRRLRIFTPTRELPFAGHPIVGTWNGLAREGVVPLPEGGTGWQRIYHEVGIGILPV
                     DIEFKNGEPVQVVMTQGQFQILDELDDSHEQAELARALGLAREDLDESLPIQTITTGL
                     PSMAVPVRSLADLRDCKINTSLLGDIYSRHGATGCLAFTRETIEVGESRAHARFFAPA
                     DNIPEDPATGSACGALGGYLIHHGALSLEPNDGRFHFVIEQGDFIHRASRVRLNVKGK
                     RGEIAEVKVGGPSVLVAKGEVVF"
BASE COUNT          261 a          297 c          247 g          220 t
ORIGIN      
        1 tggtcttggg cctttgggct tcggatgaaa tactcggacc aaagacccaa gacccaagac
       61 cgaaggccaa agtcccaaga ccgaaggcca aagtcccaag acccaaggcc aaaggccgac
      121 ccctcagaat accacttcgc ctttcgccac caacacagat ggtccaccta cttttacttc
      181 cgcaatctca ccccgtttac ctttcacgtt taatcgaacg cgactcgcac gatgaataaa
      241 gtcaccttgt tcgatcacaa agtgaaatct tccatcgtta ggctccagcg ataaagcacc
      301 atgatgaatc aggtagccac cgagagcgcc acaagcactc cccgtcgccg gatcttcggg
      361 aatgttatcg gcaggagcga aaaaacgtgc atgggcgcgc gactcgccga cctcaattgt
      421 ctcgcgcgta aaagcgaggc aaccggtagc gccatgccga gaataaatgt caccaagtag
      481 cgaggtgttg attttgcagt cgcgaagatc ggcaagagac cgaactggga cagccataga
      541 gggcagaccg gtagtaattg tttgaattgg caaactctca tccaggtctt ctcgggcaag
      601 tcctaaagct cgggccaact ccgcctgctc gtgtgaatca tccaattcat cgagtatctg
      661 aaactgacct tgcgtcatta cgacttgcac tggttcgccg ttcttgaact cgatatcgac
      721 gggcagtatg ccaatcccaa cttcatgata gatgcgctgc cagcccgtac caccttctgg
      781 cagaggaaca accccttctc gagctagtcc attccatgtt ccaacaatgg gatgacccgc
      841 aaaagggagc tcgcgggtcg gagtgaagat gcgcaaccgg cgaagcacac tcggttttga
      901 tccaatctca tctgccgctg tcgggggtgg ttcggcttta ccttcttcgc ttggaggcag
      961 aacaaagacc gtctcagata aattcatttc gcgcgcaatc tgcagcattc gctcgtcagt
     1021 tattc
//
LOCUS       DASTWE010000188         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1265460, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000188 DASTWE010000000
VERSION     DASTWE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265460"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VFT30_02070"
     CDS             complement(<1..1060)
                     /locus_tag="VFT30_02070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5091441.1"
                     /translation="MAQGSLVSQATRKIAGKALSHGIRLLAERVRANGGQPLDLVLPD
                     GSRLNFGQKPRVVLAIRDPNLLPVLTHPTLGSLGEAFVDGRIDIDGDIISVIASAERL
                     VAAGGSPAMARIPASSACHTPKQDLDDIKHHYDVSNDFYRLWLDDRMVYSCGYFQTGK
                     ETIDTAQEAKLDHICRKLRLQPGERFLDIGCGWGGLIVHAAQRYGVHAVGITLSDNQF
                     AFAKERVKAAGLERQVQVLLLDYRDAPERLGEGTFDKISSIGMFEHVGQKKFPVYFGV
                     VRRLLRDRGLFMNHGITSPDADGRTVGSGVSEFMDKYVFPHTELPHLHSVIREMARQN
                     FEVYDVESLRPHYARTLAH"
     gene            complement(1327..>1829)
                     /locus_tag="VFT30_02075"
     CDS             complement(1327..>1829)
                     /locus_tag="VFT30_02075"
                     /EC_number="1.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004128 - cytochrome-b5 reductase
                     activity, acting on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-cytochrome b5 reductase"
                     /protein_id="HEU5091442.1"
                     /translation="LTVKRYDAGSVSKYLHDRQIGDTVLMSGPNTGGHWIDGMAKRVG
                     FVAGGTGITPMISIIRWILINKIDAELFLIFANKTESDIIFRQEWGRNVRAYPNFHCH
                     LVLEQPPLEWEGSVGRVTPEILRQHLPVPDSDTCVFLCGPPPMVDSLEGVLKELCYPQ
                     QAIILP"
BASE COUNT          416 a          501 c          507 g          405 t
ORIGIN      
        1 agtgtgcgag tgtccgagca tagtgcggtc gtagactttc gacgtcatag acctcaaagt
       61 tttgtctcgc catctcccgg attaccgagt ggagatgggg aagttcggta tgtgggaaca
      121 catacttgtc cataaactcg ctcactccgg agccgaccgt ccgcccgtcg gcatcaggtg
      181 aggtgatgcc gtggttcata aacagcccgc gatcgcgcaa caggcgccgc acaacgccaa
      241 agtagacggg gaacttcttt tggccgacat gttcaaacat gccgatactc gagatcttgt
      301 cgaaagtgcc ctcccccaat ctttccggcg cgtcgcgata atccagcagt agtacctgga
      361 cctgtcgctc aaggcccgct gccttgacac gctccttggc aaaagcaaac tgattatccg
      421 agagcgtgat tccgacagca tgtaccccgt aacgctgggc ggcatgaaca atcagtccgc
      481 cccagccgca gccgatgtcg aggaaccgtt caccgggttg aaggcgtagt ttgcggcaga
      541 tgtgatcgag cttggcctct tgcgctgtgt cgatcgtttc cttcccggtc tgaaaatacc
      601 cgcaagaata aaccatgcgg tcatccagcc acagccggta aaaatcgttg ctcacgtcgt
      661 agtgatgttt aatgtcgtcg aggtcctgtt tgggagtatg gcatgccgac gaagcgggaa
      721 ttcgtgccat tgccggggag ccgccggccg ccacgaggcg ctcagcgctg gcgattacgg
      781 agatgatgtc gccgtcgata tcgatgcgac catcgacgaa ggcctcaccg agcgatccga
      841 gcgtgggatg agttaatacc ggcagcaagt tcggatcgcg gatggcgagt acaacgcgag
      901 gtttttggcc aaaattgagg cgcgagccat ccgggagtac gagatcaagt ggctggccgc
      961 cgtttgcccg gactctttcc gccaacagtc gaatgccatg actcagtgcc ttccctgcga
     1021 tctttctggt cgcctgcgag acgagactgc cctgagccat gttaacctcc tctgggtgta
     1081 acctgcgatt cttggtcgaa tgcaggtgtt cgctggagac aaattcggtt caaacgagcg
     1141 tcaaagaccc cctataccaa agagatgcga agtattgctt ctccgcttag tctttccatg
     1201 tggtcatgtc aagtgcccat gcacgcggca ctcgacgata cctatgagac gtccttctca
     1261 actgactaag gcattgtgag atttaaagta tttgaataac caggaggatg agaactagga
     1321 taatcctcag gggagaataa tcgcttgctg gggatagcat agttctttca gcacgccttc
     1381 caatgagtcg accatcggcg gtgggccgca aagaaacacg caggtgtcgg agtctggaac
     1441 gggaagatgt tgccgaagaa tttcaggggt gacgcgaccc acgctacctt cccactcaag
     1501 aggcggctgt tccaacacga gatgacaatg aaagttcgga taggctcgga cattgcgtcc
     1561 ccactcctgt cggaagataa tgtccgattc ggttttgttg gcaaagatca ggaataactc
     1621 cgcatcgatc ttgttgatca gaatccagcg aatgatggag atcatcgggg taatccccgt
     1681 gcctcccgcc acaaagccga ctcgcttcgc cattccatcg atccaatgcc cgccggtgtt
     1741 cggcccgctc atcaataccg tatcgccgat ctgtcgatcg tgcaggtatt tcgagacgga
     1801 accagcgtca tagcgcttga ccgtgagat
//
LOCUS       DASTWE010000189         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_683732, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000189 DASTWE010000000
VERSION     DASTWE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683732"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VFT30_02080"
     CDS             complement(<1..1578)
                     /locus_tag="VFT30_02080"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEU5091443.1"
                     /translation="MLGFEELFVNLRAVTLFFQKIAFDEIRFVMPFVAARVNPEGKLN
                     LMLLAPPPAETAQQPAPPSGEPKKMMPVEIDLLEITQGILEYRDDSKPRPVLIDVVPI
                     QIMLRDFSTVPSQESENAYAFKAEIGKGEQVAWEGTVSLEPVESDGKLSLSGIKLRTL
                     YQAVRDQFQFDVPQGVLGLSASYHFDLRGQAPQATVKNGKASIQNLRIVERGGIEPVV
                     NIPSFGVEGIQLDLQNQAIDVAKVHSADARFEAWMDPDGVLNYQQLFKPIGGRGAEEK
                     LPAATVKPEKPAKPWSITVDEIAIKNYGASFEDRTLERPGRVDVDAMNVTVKDVQIPF
                     KKPLPIDVSLKLNETGSVGVQGKVAVEPLSADTDLKLEHIDIRPFQPYLDRFLNADVL
                     DGAIDLVGSVHFSKEHANEPLMRFQGNLAVKQLEVADRKELDDVVTWKALKVNQIALN
                     IEPTSVKIAEIVWQEPSVYMVVDSEGQLNVSRLAAAPPPNEQTAGPNVPKDKTPVSKA
                     GEPVPVTIDQVKLVKLAA"
BASE COUNT          341 a          480 c          398 g          384 t
ORIGIN      
        1 tgcggccagc ttgaccaact tgacctggtc gatagtgaca ggcacgggct cacctgcctt
       61 cgaaactggg gtcttgtctt tgggtacgtt tggacccgcc gtctgttcgt taggcggagg
      121 cgcggcagca agacgcgaga cattgagttg cccttcagag tccaccacca tgtataccga
      181 tggttcttgc cagacaattt cagcgatctt cacgctggtc ggctcgatat tcagtgcgat
      241 ctgattcacc ttcaacgctt tccacgtcac cacatcgtca agttccttgc gatccgcgac
      301 ctccagttgc ttgaccgcca aatttccttg aaaccgcatc aacggttcgt tcgcatgttc
      361 cttagaaaaa tgcactgatc cgacgagatc gatcgcccca tccagcacat cggcattcag
      421 gaatcgatcc agatagggtt gaaagggacg gatgtcgatg tgctccagct taaggtctgt
      481 atcggcgctg agaggttcga ccgccacttt tccctgcaca ccaaccgagc cggtctcatt
      541 cagcttgagc gacacatcga tcggaagagg tttcttgaat ggaatctgca catccttcac
      601 ggtgacattc atcgcgtcta catccacacg gccgggacgc tcaagtgtcc ggtcttcgaa
      661 tgacgcgcca tagttcttga ttgcgatttc gtcgacagtg atggaccagg gtttcgccgg
      721 tttttcgggc ttgacggtcg cggctggaag tttttcctct gctcccctcc cgccgatcgg
      781 cttgaaaagc tgttgatagt tgagcacgcc atccggatcc atccaggcct cgaaccgggc
      841 atcggccgaa tgcaccttgg cgacatctat ggcctggttt tgcagatcca attggatccc
      901 ttccacacca aaactcggaa tattcacaac cggctcaatc ccaccccgtt ccacgatcct
      961 gagattctga atcgacgcct ttccattctt gaccgttgcc tgaggagcct ggccgcgaag
     1021 atcgaaatgg tacgatgcgg atagaccgag aacaccctgc gggacatcga actgaaattg
     1081 atcgcgcaca gcttgataca acgtccgcag cttgattccg gataaactca gcttgccgtc
     1141 ggactccacc ggctccagcg agacggttcc ttcccacgcc acttgttcac cctttccaat
     1201 ctccgccttg aaagcatagg cattctcgct ctcttggctt ggaacggtac tgaagtctcg
     1261 cagcatgatt tgaataggaa cgacatcgat caggaccggc cttggtttgg agtcatcacg
     1321 gtactccaaa atgccttgag tgatctccag caggtcgatc tccaccggca tcatcttttt
     1381 gggttccccc gatggtggag ccggctgttg cgcagtttca gccggcggtg gagccaacaa
     1441 catcaaattc agttttcctt caggattcac cctggctgcc acgaagggca tgacgaagcg
     1501 gatctcatcg aacgcgatct tctggaagaa cagcgtgacc gcgcgcagat tcacgaacag
     1561 ctcctcaaaa cccagcaccg ccgtctcgtt ctgctctcgc act
//
LOCUS       DASTWE010000190         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_974803, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000190 DASTWE010000000
VERSION     DASTWE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974803"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFT30_02085"
     CDS             complement(<1..744)
                     /locus_tag="VFT30_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5091444.1"
                     /translation="MSKAKFERKKPHLNIGTIGHVDHGKTTLTAAITKVLAKSGRATF
                     LAYDQIDKAPEERERGVTINIAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKADMVDDKELLELVELEVRELLSK
                     YKFPGDDTPIVIGSALKALEGDTSEMGEGAILKLMEAVDSFVQEPEREIDKPFIMPVE
                     DVFTISGRGTVVPGRVERGIIKVGEEIEIV"
     gene            complement(831..903)
                     /locus_tag="VFT30_02090"
     tRNA            complement(831..903)
                     /locus_tag="VFT30_02090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(868..870),aa:Thr,seq:ggt)
     gene            complement(995..1070)
                     /locus_tag="VFT30_02095"
     tRNA            complement(995..1070)
                     /locus_tag="VFT30_02095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1035..1037),aa:Gly,seq:tcc)
     gene            complement(1076..1160)
                     /locus_tag="VFT30_02100"
     tRNA            complement(1076..1160)
                     /locus_tag="VFT30_02100"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1124..1126),aa:Tyr,seq:gta)
BASE COUNT          285 a          356 c          303 g          284 t
ORIGIN      
        1 gacaatttct atttcttcgc caaccttaat aatgcctcgc tcaacccggc cggggaccac
       61 cgtgccgcgc ccactaatgg taaaaacgtc ttcgacgggc ataatgaagg gtttatcgat
      121 ttcgcgttca ggctcctgaa cgaacgagtc aacggcttcc atcagcttca agatcgcgcc
      181 ctcacccatt tcggaggtat cgccttccag agccttgagc gcgctgccaa tgacgatcgg
      241 tgtgtcatcg ccgggaaact tatatttgga aagtagctcg cgaacttcaa gctcgaccag
      301 ctccaacagc tctttgtcat cgaccatatc ggccttattt aagaagacga caatggcagg
      361 aacgccgact tggcgtgcca ggaggatatg ctctcgggtt tgcggcatag gaccatcggc
      421 ggcggaaacc accaaaatcg cgccatccat ttgcgccgca ccagtgatca tgttctttac
      481 atagtcggca tgtccagggc aatccacgtg agcgtagtgg cgcttgtcgg tctcatactc
      541 gacatgggca atgttgatgg taacgccgcg ttctctttct tcgggcgcct tgtcgatctg
      601 atcataggct aagaaagtgg ctctacctga cttcgccagg accttggtaa tcgccgcggt
      661 cagagttgtc ttaccatggt ctacgtgacc gatcgtacca atgttcaaat gtggtttctt
      721 acgctcaaat tttgctttgc tcatcgcgtc ctcctaattg atttccaaat catcaccctt
      781 aacgtttcaa ctaatgaacg actctcccca atgtcgcgac tgaaaggcaa agcccaggag
      841 cgggattgaa ccgctgacct cgtccttacc aaggacgtgc tctaccgact gagctacctg
      901 ggcatatcat tggatgacgc ccaggccgtg cggtgcccac gatcggggcg agagatcggc
      961 ctcgcaacaa cgtcatcctg gataaatacc aatatggagc gggaaacggg actcgaaccc
     1021 gcgaccccaa gcttggaagg cttgcgctct accaactgag ctattcccgc aaaattggtg
     1081 gagagggaag gattcgaacc ttcgaaggca tcgccggcag atttacagtc tgctcccttt
     1141 ggccactcgg gaacctctcc tcgactccac ccagaaacaa actcttcgcc acaccaacct
     1201 tcctcaatgg agtaggtgga gctggcga
//
LOCUS       DASTWE010000191         1828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1007187, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000191 DASTWE010000000
VERSION     DASTWE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007187"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..421)
                     /locus_tag="VFT30_02105"
     tRNA            complement(346..421)
                     /locus_tag="VFT30_02105"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Thr,seq:tgt)
     gene            605..>1828
                     /locus_tag="VFT30_02110"
     CDS             605..>1828
                     /locus_tag="VFT30_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5091445.1"
                     /translation="MNQLHVLVVDDDSAVRDVLQEALTQEEYSVSTAEDGAAAIQAVK
                     DSVVHIVITDFQLPDIDGLEIIDRLAKLDAKIIPIMMTGFGTIETAVRAMKSGAFDFI
                     TKPFDLEAVAVVVRKAAEFLRLRQENHLLRKAVRDQYRLEQLVGASEPIQQVMEFVRK
                     VADSDSTVMIQGESGTGKELVARMLHFNSLRKDRPLVPVNCGAIPENLLESELFGHEK
                     GAFTGATHARMGRFELANGGTIFLDEIGEMSLPLQVKLLRVLQEREFERVGGNRTIHV
                     DVRIVAATNQDLETLVEEKRFRKDLFYRLNVIPIVIPPLRERRSDIPLLIDHFLTRFN
                     QTKHTEISSLAPDALHLLTEYDWPGNIRELENMIERLVVLKKRGVLSIGDLPEKICRR
                     SSGSELTERFIRFNED"
BASE COUNT          426 a          487 c          505 g          410 t
ORIGIN      
        1 ccataccgag tgttcaatcg tgctgagcgt gttgagaacg gctcaattgc ctctgcactt
       61 cacgccgcat tgtctccgcc acatctatgc ctcctcctgc ttgcagaaag cgtcagccct
      121 gctcatgtcc aggagcaatt aggccgtgcc acgattgaat tgtcagtttc cacttatggt
      181 cgatggttga ggaaaagagc gcctggtgca gttggatcgg ctcgactcag ccgaggagaa
      241 atacgtagct gcccaacctt gtggtagcaa aacggtagca ggcgtggctt atgtaccgaa
      301 cagaccgaac tgattcttca actacctgaa atgtccaaga aggaatggag ctggcgagag
      361 gaatcgaacc tccaacctgc ggtttacaaa accgctgctc tgccgattga gctacgccag
      421 cccgctcata agatcgcgtt cgaactttgt ctcgcacagt accaataggc cgaagaacct
      481 gtcaagaaag gcctctcgtc ctagataggt cgattgctgg gtttattgat acggcttgca
      541 ttgcttgctg cgggccatta ttctaggctc gttttctcct ttcgtattcc aaaggacatc
      601 aagtatgaat caactccacg tcctggttgt cgatgacgac tctgccgtca gggatgttct
      661 tcaggaagcc ctcacccagg aggagtatag cgtctccact gccgaggacg gcgccgcggc
      721 aatccaggcg gtgaaggact cggtggtcca tatcgtgatc acggattttc agttgcccga
      781 tatcgacgga ctcgagatca tcgatcgcct tgcgaaactc gatgcgaaga tcattcccat
      841 catgatgacc ggattcggca ccatcgaaac agcggtccgt gccatgaagt ccggcgcgtt
      901 cgacttcatt accaagccgt ttgatctgga agccgtggct gtggtggttc ggaaagcggc
      961 ggagttcctt cggcttcggc aagaaaacca cctcttgcgg aaggctgtac gcgaccaata
     1021 tcggttggaa cagctggtag gtgccagcga gccgatccaa caagtaatgg agttcgtccg
     1081 aaaagtggcc gacagcgaca gcacggtcat gattcaaggc gagagcggca ccggcaagga
     1141 gttggtggct cgcatgctcc acttcaacag tctccggaag gatcgtccgc tggtcccggt
     1201 gaattgtggc gccattcctg aaaatctgtt ggagtcggaa ctcttcgggc acgaaaaagg
     1261 cgcgttcaca ggagccactc atgcgaggat gggccggttt gaactggcaa acggagggac
     1321 catctttctc gatgaaatcg gcgaaatgag cttgccgctg caagtgaagc ttcttcgggt
     1381 attacaggaa cgggaattcg aacgagtcgg aggaaatcgg acgattcatg tggatgtgcg
     1441 tatcgttgcc gcgaccaatc aggatttaga aacgctggtc gaggaaaagc gatttcgcaa
     1501 agacctgttc tatcggctca acgtgattcc catcgtgatc cctccgctac gggaacgccg
     1561 aagtgacatt ccgctcctga tcgaccattt tctgactcgc ttcaatcaaa ccaagcacac
     1621 cgagatttca agccttgccc ccgacgccct ccatctattg accgagtatg actggcccgg
     1681 caacattcgg gagttagaaa acatgatcga gcggttggtc gtgctgaaga agcgcggagt
     1741 cctttccatc ggcgatttac ccgagaaaat ctgtcggagg tcgtcgggct ccgaactcac
     1801 ggagcggttc atccggttca acgaggac
//
LOCUS       DASTWE010000192         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1265562, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000192 DASTWE010000000
VERSION     DASTWE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265562"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFT30_02115"
     CDS             <1..529
                     /locus_tag="VFT30_02115"
                     /inference="COORDINATES: protein motif:HMM:NF020803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HEU5091446.1"
                     /translation="GGRVNRIDTRAGTVHTDFDEYKPAVANVIPPQWASSIARVAALD
                     DGKGWCPIHASTFESTVHRDVHIIGDAAIVNPIPKSAFGANIEAKACAAAIASLLRAE
                     PVAPPVLMNTCYSLVAPNYGISITGSYRVVDDTIHTIPGTDGISPLDAPDEIRAQEAQ
                     YARSWYANITADTFT"
     gene            526..>1243
                     /gene="soxX"
                     /locus_tag="VFT30_02120"
     CDS             526..>1243
                     /gene="soxX"
                     /locus_tag="VFT30_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEU5091447.1"
                     /translation="MTNYVRLIRGLIVVCLTVLPGTDVRSSLAETEKVQIHYTVKDGA
                     IVEPLTRTAGDSDRGKQIVLDRERGDCLVCHTVPLPDRQMHGTVGPLLDGVGSRYSAE
                     ALRLRLVDSKVINPQSLMPAYHRVSKLYRVLKPYRDRPLLTAQEIEDVIAYLSTLTHT
                     SPISNGQKMRQPATGQLKTEPRKPAQNLSHGDTRRSGYTYLSQENQQLQDDEFANPGM
                     LWVERGRELWAKPDGAAHVAC"
BASE COUNT          296 a          362 c          347 g          238 t
ORIGIN      
        1 aggaggtcgg gtgaaccgga tcgacacgcg tgcgggtacc gtccacacag acttcgacga
       61 atataagccg gccgtggcaa acgtcattcc tccgcagtgg gcctcgtcga tcgcaagagt
      121 cgcagcgctc gatgacggaa aaggatggtg tccgatacac gccagcactt tcgaatcgac
      181 cgtacatcga gacgttcata tcatcggcga cgccgcgatc gtgaatccga ttcccaagtc
      241 agcattcggg gccaacatag aagcaaaagc ctgtgcggcc gcgatcgcgt cactcctacg
      301 cgccgaacct gtggcacccc cagtgcttat gaatacctgc tacagcctcg tcgctcctaa
      361 ttacggcatc tcgattaccg gtagttatcg agtcgtcgac gacacgatcc acacgatccc
      421 ggggactgac ggaatcagtc cgctcgacgc gccggacgaa attcgtgccc aagaggcaca
      481 gtacgcgcgt tcgtggtacg ccaatattac ggcagacaca ttcacatgac gaattacgta
      541 cggttgatcc gaggactcat cgtcgtgtgt ctgacagttc tgccgggcac tgacgtccgt
      601 agtagcctgg cagaaactga gaaagtgcaa attcactaca ctgtcaaaga cggggcgatt
      661 gttgaaccac tcacccgtac tgcaggtgac tctgatcgtg ggaagcaaat cgtgctggat
      721 cgcgagcggg gcgactgtct tgtgtgtcac accgtgccac tgccagaccg ccagatgcat
      781 ggcacggtgg gtccgctgct tgacggggta gggagtcgct actcggcaga ggcactacgg
      841 ttacgcctcg tggattcaaa ggtgatcaat ccacagtcac tcatgccggc ctatcaccga
      901 gtgagcaaac tttatcgcgt gcttaagccg tatcgcgata gaccattact gacggcccag
      961 gagattgagg acgtcatcgc gtatttgtcg accctcacgc acacatcacc catctcgaac
     1021 ggacagaaga tgcggcaacc ggcaaccggg caactaaaaa cagaacctcg gaaaccggct
     1081 cagaacctct cccatggaga cactcgccgt tcaggctaca cctacctgtc gcaagagaac
     1141 caacaactgc aagatgacga gttcgcgaat ccgggtatgt tgtgggtgga acgcggccga
     1201 gagttatggg ccaagcccga tggcgctgct cacgtggctt gtg
//
LOCUS       DASTWE010000193         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_877840, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000193 DASTWE010000000
VERSION     DASTWE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877840"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFT30_02125"
     CDS             <1..288
                     /locus_tag="VFT30_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091448.1"
                     /translation="IVDCDDWLSINLIGFDWYANWDIAQRVGGRSPKHIAGRTFIRGR
                     LGDEGMCNTVPRKIMKGFVVGHLTFGPQNLTEHQQRVCRKMYEEIVDEYQS"
     gene            285..986
                     /locus_tag="VFT30_02130"
     CDS             285..986
                     /locus_tag="VFT30_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091449.1"
                     /translation="MKVEYIIPFRDRGIDQLRHKNLMCVTDYWRANGVHWPHVCTDGR
                     AGDAQFNRSAAYNFGVRNHDADVYVFTESDMIVPKDQIDKAIEMAVESPGLVVPFTLY
                     CYLAPDDSALVRADQIKAHTCSPVYTKENGRSIGAVNVVSRETMEMVGKWDENFEGNW
                     YDDDAMKRAFDVAAAKTRWVEGPAYHLYHMPGWKGEHLTDEDRAATKRNKKRYLKYRQ
                     ATTPEQIRKLTSGGS"
BASE COUNT          310 a          259 c          328 g          249 t
ORIGIN      
        1 attgtcgact gtgacgactg gttgtcgatc aaccttatcg gattcgactg gtatgcaaac
       61 tgggacatcg ctcagcgtgt aggtggacga tcaccgaagc acatcgctgg tcgcacgttc
      121 attcgtggtc ggctcggtga tgaaggcatg tgcaacactg tacctcgaaa gatcatgaaa
      181 ggtttcgtgg tcggtcatct cacgtttggt ccgcaaaatc tgacagagca tcaacagcgc
      241 gtgtgccgca agatgtacga agaaatcgtg gatgagtacc aatcatgaaa gtcgaataca
      301 tcatcccatt tcgtgaccgc ggtatcgacc agctgcgcca caagaatctg atgtgcgtaa
      361 cggactactg gagagctaac ggggttcatt ggcctcacgt atgcaccgat ggtcgtgctg
      421 gtgacgctca gttcaatcga tcagctgcgt acaattttgg cgtgcgcaat catgacgcgg
      481 atgtctacgt gtttactgag tcagacatga ttgtgccgaa agatcagata gacaaggcga
      541 ttgagatggc cgtggagtcg ccaggactgg ttgtgccatt cacattgtat tgctatcttg
      601 cgccagatga ttcagcactc gttcgtgccg accagatcaa agcacataca tgctctcctg
      661 tgtacaccaa ggaaaatgga cgcagcattg gtgctgtgaa cgtcgtgtct cgagagacca
      721 tggagatggt cggcaaatgg gatgagaatt tcgaaggcaa ctggtacgac gacgatgcga
      781 tgaagcgcgc gtttgatgtg gcagcggcta aaacgcggtg ggttgaaggc ccggcgtacc
      841 atttgtacca catgccagga tggaaaggag agcatctcac agacgaagat cgggcagcga
      901 cgaagagaaa caagaagcgc tacctgaaat accgacaagc aacgacacca gagcagatac
      961 ggaaactaac tagtggtggg tcatgacaaa cataagctat gtagtagtcg gagatacgcg
     1021 cagatcacgc agagcattta gtcttgcacg aagaattgat gcgtcagtgg cactggacct
     1081 tgaaggtact tggggccctg gaatcaatca cacaagagca tgggttcttg gaaacaccga
     1141 gaaatg
//
LOCUS       DASTWE010000194         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_263954, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000194 DASTWE010000000
VERSION     DASTWE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263954"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..>1136
                     /gene="dnaE"
                     /locus_tag="VFT30_02135"
     CDS             3..>1136
                     /gene="dnaE"
                     /locus_tag="VFT30_02135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5091450.1"
                     /translation="MVSKAYLEGFYYKPRMDKELMQEHHEGLIGLSGCLSGEVSYLIG
                     QQDLDGAIRAAGEYREIFGKDNYYLELQANGLEHQRIANDGLLEIHKKLGIPLAGTND
                     CHYLKKEDSRPHDLMLCLQTGKTINDPNRMKFDTDELYVKSTDQALAEFREMPTAVSN
                     TVKIAEACSLDLALNETYLPQFKVPDGFTRETYVEELAKQGLAARLKERPSSIPEIAY
                     QVRLKEEVTVICSMGFAGYFLIVWDIIKFARSRGIPVGPGRGSAAGSLVAYALRITDL
                     DPLVYSLLFERFLNPERVSLPDIDMDFCMDRRDEVINYVIHKYGEDHVAQIITFGTMK
                     AKAAIRDVGRVLEMPYAEVDRIAKLVPDDLKMTLDKALEQEPRL"
BASE COUNT          267 a          313 c          312 g          244 t
ORIGIN      
        1 agctggtaag caaagcatat cttgaaggtt tctactataa gcctcggatg gataaggaat
       61 tgatgcagga acaccatgag gggctcatcg gcctttcagg ttgtctgagc ggggaagtgt
      121 cgtatctcat tgggcaacag gatttggacg gtgccattcg ggccgcgggc gagtatcgcg
      181 aaatctttgg gaaggacaat tactacctag aactgcaagc caatggactc gagcatcaac
      241 gcatcgcgaa tgatggactc ctggagattc ataaaaaatt gggcattccc ctcgccggca
      301 ccaacgattg ccattatctc aagaaagaag attcccgccc gcatgacctc atgctctgcc
      361 tacagacggg gaagaccatc aatgatccga accggatgaa gtttgacacc gatgagctct
      421 atgtcaaatc gacggatcag gcgctcgcgg aattcaggga aatgccgacc gccgtctcca
      481 acaccgtcaa gatcgctgag gcctgctcac tcgacttggc gctcaacgaa acctacctgc
      541 cgcagttcaa ggtccccgac ggtttcaccc gtgaaaccta cgtagaagaa ctagcaaagc
      601 aaggcctggc tgcgcgcttg aaagagcgtc cgagttccat tcccgaaata gcctatcagg
      661 tgaggctcaa agaagaagtc acggtaatct gttcgatggg gttcgccggc tatttcctta
      721 ttgtctggga catcatcaaa ttcgcccgct cgcgcggcat ccccgtggga ccgggtcgcg
      781 gctccgccgc gggcagtctt gtcgcctacg ccttgcgtat tactgatctc gatccgctcg
      841 tgtactcgct gttgttcgag cgttttttga accccgagcg cgtttccctt cccgacatcg
      901 acatggactt ctgcatggat cgccgcgacg aggtcatcaa ctatgtgatt cacaaatacg
      961 gcgaggacca tgtcgctcag atcattacct tcggaaccat gaaggccaag gcggcgatac
     1021 gcgatgtcgg ccgtgtgctc gaaatgccgt atgccgaggt ggatcggatc gcgaagctgg
     1081 tgcctgacga cttgaagatg acgctcgaca aggcgctcga acaggagccg cgcctg
//
LOCUS       DASTWE010000195         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1362708, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000195 DASTWE010000000
VERSION     DASTWE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362708"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..890
                     /locus_tag="VFT30_02140"
     CDS             6..890
                     /locus_tag="VFT30_02140"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5091451.1"
                     /translation="MCAYLGQKLGEATVSSFSDGEIRVKIDENVRGADVFVVQSCCQP
                     VNDSLMELLIIIDALKRSSANRITAVIPYFGYARQDRKDQPRVPITAKLVADLMTTAG
                     ADRVLSMDLHAGQIQGFFNVPVDHLYALPVLLDYIVKKKISDLVVVSPDAGGVERARA
                     FAKRLQANLAIIDKRREGPNQAQIMNIIGDVQGTSVLLLDDMIDTAGTIVQGAQACLD
                     HGAREVITACTHAVLSGPALERLQASCLSQVVVTNTIPLRGKELACPKLHQLSVAPLL
                     GEAIRRIHEDESVSSLFA"
     gene            943..1725
                     /locus_tag="VFT30_02145"
     CDS             943..1725
                     /locus_tag="VFT30_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU5091452.1"
                     /translation="MKFDLTVAVREEAGKGAARSMRRAGKIPAVLYGQGECLLLTVNP
                     EELVKILKSHAGSTALISLTVNGAKSKQSRTALLRDYQVDPVTGAVLHADLFEISMSK
                     PIRVKVPIKVIGGIPAGVKEGGVLHHNMRDVHVECLPAALPDHIEVDASALTIGSGIH
                     VKEIGAREGVRFLDDADQMVVSVAAPMSDAKLEALLTSTAAAPGEPEVLTKGKEVAGA
                     EGTGAEPAKAGAAAPAGEAKGGEKKEAAAAPKAEKKEAEKKK"
BASE COUNT          440 a          495 c          568 g          373 t
ORIGIN      
        1 atgagatttg cgcttacctc ggacagaagt tgggtgaggc gacggtctcg tctttcagcg
       61 atggagaaat tcgcgtcaag atcgacgaaa atgtgcgtgg cgccgacgtg ttcgttgtgc
      121 agtcctgttg tcaaccggtc aacgattcct tgatggagtt gctgatcatc attgatgcgc
      181 tgaaacgttc ttcagccaat cgtatcacgg ccgtcatccc ctatttcgga tatgctcgtc
      241 aggatcgcaa ggatcaaccg cgcgtcccca tcaccgcaaa gttggtcgcg gatttaatga
      301 ctaccgcagg agccgatcgt gtgttgtcga tggatcttca cgccggacaa atccagggat
      361 ttttcaatgt gccggttgac catttgtatg cacttccggt gttgctggac tacattgtga
      421 agaaaaaaat atcggatcta gtcgttgtct cgcccgatgc aggtggagtc gaacgagccc
      481 gagcattcgc caagcggctt caagccaacc tggcaattat cgacaagcga cgcgaaggtc
      541 caaaccaagc gcaaataatg aacatcatcg gggacgtgca agggacaagc gtgttgctcc
      601 tcgatgacat gatcgatacg gcaggcacta ttgtccaagg ggctcaggcc tgtctcgatc
      661 atggcgctcg agaggtgatc acggcctgca cccacgcagt gctgtcgggg ccagctctgg
      721 aacggttaca ggcgtcatgt ctctctcaag tggtagtgac caacacgatt ccactgcgag
      781 ggaaagagtt ggcctgtccc aaattgcatc aattgtcggt agcgcctctg ctgggcgaag
      841 ccatcagacg cattcatgaa gacgagtcgg tgagttccct attcgcctaa gccattatcg
      901 attgggcgct gcgtaagaag caaaggagat ggagggagga tcatgaaatt cgatttgaca
      961 gtggcagtaa gagaagaagc gggaaaaggg gccgcgcggt ccatgcggcg ggcgggaaaa
     1021 atcccggccg tgctctacgg ccaaggggaa tgtctcttgc tgaccgtcaa tccggaggaa
     1081 ttagtcaaga tcctcaagtc tcatgcgggc agcaccgcgc tcatttcgct cacggtgaac
     1141 ggagcaaagt ccaagcagag tcgcacggcc ctcttgcgcg actatcaagt cgacccggtg
     1201 accggggctg tcctgcacgc ggacctcttt gagatttcca tgagcaagcc gattcgggtg
     1261 aaagttccga tcaaagtcat cggcgggatc ccagccggcg tcaaggaggg cggcgtgctg
     1321 caccacaaca tgcgcgatgt gcacgttgaa tgtcttccgg ccgctctgcc tgatcacata
     1381 gaagtcgatg cgtcggcatt gactatcggc agcggcatcc atgtgaaaga aatcggagca
     1441 cgcgaaggtg tccgtttctt ggatgatgcc gatcagatgg ttgtcagcgt cgctgccccg
     1501 atgtcggatg ccaaactcga agccttgctc accagcaccg cggcagcgcc aggagagcct
     1561 gaagtcctga caaaaggcaa ggaagtggct ggggctgagg gaaccggcgc cgaaccagcc
     1621 aaagccggtg cggcagcgcc ggccggtgaa gcgaagggtg gcgagaagaa agaagctgca
     1681 gccgctccaa aggcagaaaa gaaagaagct gaaaagaaga agtaacgttg cacctgctcg
     1741 ttggactggg caatcccgga aaagcctacg cccagacccg tcacaatgtc ggcatgtggg
     1801 tcatcgagcg ggctgccgct cgatggtcga tccggctctc accgcgcggc acagctcaac
     1861 gaggttccgg gcggct
//
LOCUS       DASTWE010000196         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1233444, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000196 DASTWE010000000
VERSION     DASTWE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233444"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFT30_02150"
     CDS             <1..580
                     /locus_tag="VFT30_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEU5091453.1"
                     /translation="AYYNAPPTTALPVVRQAKEGGSRELVLMTWDLIPRFSKDGKPSF
                     STINARAEGLHTAASYKQPFAEGRRCLAPASGYFEWTGPKNDRQPHYFTRADGQLIAL
                     AGLWDRWRSKDKSETKESFTIVTTEPSKFAAQFHNRMPLVLEPDAWDLWMNGDPEAAA
                     ALMRPANEDVLAERSVSKAVGNVKNNGPELLA"
     gene            complement(577..>1066)
                     /locus_tag="VFT30_02155"
     CDS             complement(577..>1066)
                     /locus_tag="VFT30_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091454.1"
                     /translation="SSSLYIYRFGSIFNAYRLIGYPGGSRNVAWNVRFSMRTVRSKII
                     DEMRLRLQSQGLDIQSNPTVTNLMISNGIACTFGVSKPYEVSSGRRWIFRCHTKFHLP
                     FHLYGRLDVDEQTVFDYYLVPRSALDLMPLRMKLRNAPEVEDFHAASLNIALKRLVRS
                     VV"
BASE COUNT          255 a          296 c          305 g          210 t
ORIGIN      
        1 ggcctactac aatgcgccgc cgaccacggc attgcctgtc gtgcgccagg cgaaggaagg
       61 cggcagtcgc gagctggtgc tgatgacctg ggacctcatc cccaggttct ccaaggatgg
      121 caaaccctcg ttctcgacca tcaacgcacg cgctgaaggc ttacacactg ctgcgtccta
      181 taaacagccg ttcgccgagg gacggcgctg cttggcgcca gcatcgggtt acttcgaatg
      241 gacagggccg aagaacgatc gacagccgca ctacttcacg cgtgctgatg gtcagctgat
      301 cgcattagct ggtctgtggg atcgctggcg aagcaaggac aagtcagaga ccaaggaaag
      361 cttcaccatc gtcacgacag agcccagcaa gtttgctgct cagttccaca atcgcatgcc
      421 gctcgtgctg gagccggacg cctgggatct gtggatgaac ggtgatccag aagctgcagc
      481 agcgctaatg aggcctgcga acgaggatgt attggcggag cggtctgtgt cgaaggcggt
      541 cggcaacgtg aagaataacg ggccagagct actggcctaa accacagatc gtaccagccg
      601 tttgagcgca atattcaacg acgcagcatg aaagtcctct acctccggag catttctcaa
      661 cttcattcta agcggcatca aatcgagagc cgaccgcgga acgaggtagt agtcaaaaac
      721 ggtctgttcg tccacgtcga gccgaccgta aagatggaac ggcagatgaa acttggtatg
      781 gcacctgaag atccatcttc ggccactcga cacctcgtag ggtttcgaga ctccaaaagt
      841 gcacgcgatc ccgttgctta tcatgaggtt tgtgacggtg ggatttgact gaatgtccaa
      901 gccttggctc tgcagccgta accgcatctc atcgatgatc ttagaccgca ctgttcgcat
      961 gctaaacctg acgttccacg cgacgtttcg tgacccgcca ggatacccga tgagacgata
     1021 agcgttgaag atgctgccaa accggtagat gtagagcgat gaggac
//
LOCUS       DASTWE010000197         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_942671, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000197 DASTWE010000000
VERSION     DASTWE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942671"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFT30_02160"
     CDS             complement(<1..305)
                     /locus_tag="VFT30_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5091455.1"
                     /translation="MTDSNEQEIQPPQNIEVPGQLPLLPVRDIVVFPYMVLPLFVGRD
                     MSIKAIEAALAGNRMIFLATQKALDVENPAPKDIHAIGTVGIIMRMLKLPDERIKILV
                     "
     gene            complement(554..1450)
                     /gene="galU"
                     /locus_tag="VFT30_02165"
     CDS             complement(554..1450)
                     /gene="galU"
                     /locus_tag="VFT30_02165"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU5091456.1"
                     /translation="MSRLTVRKAVIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYTV
                     EEAVASGIEDIIVITGRGKRAIEDHFDRSVELEENLKGTGKSQVLHQIRQISNLANFC
                     YVRQSEALGLGHAVLCAQHLIGDEPFAVILGDEIIDADVPGLAQLIHVYKKRHGAVLG
                     VQEVPKADVSRYGIVTPKRLAHGLHRVEDLVEKPSPTDAPSTLAVIGRYVLPPEIFPI
                     LRKTAPGKNGEIQLTDALKTLAKKSPMYALEIEGQRHDAGDKLGFLIATVEFALKNPA
                     LGAEFRDYLSTKMHTASNNRRG"
BASE COUNT          446 a          520 c          603 g          496 t
ORIGIN      
        1 accaggatct tgatgcgttc atcaggcagc ttcagcatcc gcatgatgat gccgacggtg
       61 ccgatcgcgt ggatgtcttt gggggcggga ttttccacat cgagcgcttt ctgagtcgcg
      121 aggaagatca tacgattgcc ggcaagggcc gcttcgatgg ccttgatcga catgtcgcgt
      181 ccgacgaaaa ggggaagcac catatacgga aaaacgacga tatcccggac cggcaacagc
      241 ggaagctgac cgggaacctc aatgttttgt ggaggctgaa tttcttgttc gtttgagtcg
      301 gtcatgtgcc tgcctgttct ttaactctaa gaggtggcgg gttcgacgaa ccgggttgtg
      361 caacgactca tcatgcgtct gagcgacatg atctttctca tctagaccct cagtgttcca
      421 tcctattgga acgcgattga ccgatcggga ttacgcgatg cgccgggtcg gacaattcgt
      481 ccaatatcaa gccgggtgca gaatggtcgt tgccgggaat ctcgcgactg agtgcccatc
      541 atgactgatg ggatcatcct cgacgattat tcgatgcggt gtgcattttg gtcgaaaggt
      601 agtcgcggaa ttctgctccc aatgccgggt tcttcaatgc gaactcaacg gtagcgatca
      661 aaaagcccaa tttgtctccg gcgtcatgtc gctgtccctc tatctccagc gcatacattg
      721 gagatttttt tgccagtgtc ttcagtgcat cggtcaactg aatttccccg ttctttccag
      781 gggcggtttt tctcaagatc ggaaagattt cgggaggaag gacataccgg ccgatcacgg
      841 cgagcgtaga tggggcatcg gtcggcgatg gtttctcaac caaatcctca acccgatgca
      901 gaccgtgagc gagacgcttg ggggtcacaa ttccgtaccg gctgacatct gccttgggaa
      961 cctcctgcac tcctagaacc gccccatggc gttttttata gacgtggatc aattgcgcga
     1021 gccctgggac atcggcgtcg atgatctcat ctccgagaat gacggcgaag ggttcatcgc
     1081 cgattaggtg ttgcgcgcat aacacggcat gacccaagcc gagggcttcg gattgccgca
     1141 cgtaacagaa gttcgcaagg ttcgagatct gtcggatttg atggagcacc tgactcttgc
     1201 cggtgccttt caaattttct tccaactcaa cggaacgatc aaagtgatct tcgatggcgc
     1261 gtttgccgcg gccggtgatc acgataatgt cctcgatgcc cgaagctacc gcttcttcga
     1321 ccgtatattg aatgagcggt ttgtcgacca atggcaacat ctctttgggg gaggcctttg
     1381 tggcgggaag gaaccgtgtc ccaaggccgg cggcgggaat gaccgctttt ctgacagtca
     1441 ggcgtgacat ggaaggatac tatgtgatgg ggtgtgtgaa gtcaagaaac ggacgaactc
     1501 atcgcatcga aggcgatatt gtcattcctg tgaaaagaag acacaagtga tctcatgaac
     1561 gagatgtgtg tcgtgccgtt tctccggaat ggcaggtggc tgtcatgatc gatgcgatcc
     1621 ttgggaaacg tcgcgatcag agccaagcaa gatgtgccca cgtggaatca gaggacggga
     1681 cactccttta caatgcagga tgacctcaat ataatccgga agttttgcga ggtcgggtga
     1741 tgcaaggatg ttcgtgtggt agagctgccg agttcgaatt cccgacgagg atgctcttgc
     1801 ggttcagggc gctctccgga gcgggacgga ctgactcgta gggagttggc gtacggtcga
     1861 taacggagag gacctgtgtg atggttccaa ccgcatcatc catctccata cttcgccaca
     1921 ggaaccctta tcggtgaccg agcatgccgt ctacagacct cgatcgttcg tggtcgcggt
     1981 catggttgtc tccgtgttgt ggagcgtgct cgaagcactc gctcagacgc ccgacctcaa
     2041 ggtcgcttcg attgaaattc ggggc
//
LOCUS       DASTWE010000198         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_749008, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000198 DASTWE010000000
VERSION     DASTWE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749008"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFT30_02170"
     CDS             complement(<1..697)
                     /locus_tag="VFT30_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEU5091457.1"
                     /translation="MLEPTMTPSLMNAASEAVAATVPPIDFLGYWTTDCREVKTFRGH
                     SHGVWAVAFSPDGSMLASGGAERLVRMWDIETGRLLRSLRGHTNDIRAIVFTPDGQTL
                     ATGSEDRTIRLWNGKTGEPMKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIKIWEIT
                     TGTELMTLLGKDEYDHHWSVCVAFSPDGIHLASGTDIGKIKVWEVLPSGEEKVLHDGH
                     WRRNQEDSTETRGY"
     gene            975..>1346
                     /locus_tag="VFT30_02175"
     CDS             975..>1346
                     /locus_tag="VFT30_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091458.1"
                     /translation="MEIRFQPALLQEVIDSFVEKTEREGDPTYYKEFHEHADPIYEKF
                     ILEDREAEFKKLYQYLFGIWGFSDIVRDSFNEYPLLKEKVGIVLVKGVLKEDQEGVDI
                     LRKWGSVEKGLAQEFEAKGMKG"
BASE COUNT          292 a          347 c          399 g          308 t
ORIGIN      
        1 agtagccgcg ggtctcggtg gaatcttcct ggttccgccg ccaatggccg tcgtgcagaa
       61 ccttttcctc gccgctcggg aggacctccc atactttgat cttgccgatg tcggttccgc
      121 tcgcgagatg aataccgtcc ggcgagaagg ccacacagac cgaccagtga tggtcatact
      181 catccttccc gagtaacgtc atgagctcgg tgccggtggt gatttcccaa atcttaatgt
      241 ctttgttgtg gctgccgcgc gcgagcatga ggccgtccgg cgagagcgaa aggctgcaga
      301 cgttgtgatc gtagcgtgta aacagcagct tcatgggctc gccggtcttg ccgttccata
      361 accgaatggt gcggtcctca ctaccggtgg ccagggtctg tccgtccggg gtgaaaacga
      421 tagcgcgaat atcattggtg tggccgcgca gggaacggag caaccgaccg gtttcgatgt
      481 cccacatgcg cacgaggcgt tcggcgccac cgctggcgag cattgagcca tcaggtgaaa
      541 acgcgaccgc ccaaactccg tgcgaatgac ctcggaatgt tttcacttcc cgacagtcgg
      601 tcgtccagta tcctaagaaa tcgataggag gtaccgtggc ggctaccgct tcagaagcgg
      661 cgttcatgag cgaaggtgtc attgtgggtt ccaacattgg tagattgctt ttcttggccg
      721 cctggtcctt gattgggcaa cagcccggcc agtataattc cgtcggtcgt tcggatcgta
      781 agagatgctt cgcggccaag tcaacccttc ggctaattgg tctgttgaga ggggaggctc
      841 agggttgacg ctgagcgact ttctaaacac cgtcgaagcg tcaagctggc cccccatcac
      901 catgtcgtcg atgggttgtc gcataggata tctcaaagtg gccccctcaa gctcaagcct
      961 ctgaggattt ttcaatggaa atcagattcc aaccggcttt gcttcaggaa gtgatcgact
     1021 cgttcgttga gaagacggaa cgggaaggcg atcctacgta ctacaaggaa ttccacgagc
     1081 atgcggaccc catttacgag aaattcatcc tcgaagaccg ggaggcggag ttcaaaaaac
     1141 tctatcaata tctttttggc atctgggggt tttcggatat cgttcgcgac tcgttcaatg
     1201 agtatccact cttgaaggag aaggtgggaa ttgtcctggt caaaggtgtg ctgaaggaag
     1261 accaggaagg cgtcgatatc ctccggaaat gggggtctgt cgagaaaggc ttggctcaag
     1321 agtttgaggc gaagggaatg aagggc
//
LOCUS       DASTWE010000199         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1556710, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000199 DASTWE010000000
VERSION     DASTWE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556710"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1137)
                     /locus_tag="VFT30_02180"
     CDS             complement(<1..>1137)
                     /locus_tag="VFT30_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEU5091459.1"
                     /translation="ASYPTLLLITTLYRLSLDISATRLILLQADAGEVIRAFGMFVVS
                     GNFVVGAVIFLIITLVQFIVITKGAERVAEVSARFTLDAMPGKQMSIDADIRAGIIDF
                     QEGKKRRDALSRESQFYGAMDGAMKFVKGDAIAGIIITLINIIGGLIIGMAMKNMELM
                     KAVQTYSILTIGNGLVSQIPALLISISAGMVITRVASETSDSNLGKDVANQILAQPKA
                     IAVSSGLLLVLAVIPGLPKIPFFLLAGVTGSVAYGLFRARKMQAIEETAQTGTADRGQ
                     EPQLTVTVPLVLEVSEPLTPFVNTANAEGKSFVEKLTDVRNSLYYDLGVIFPPIQISG
                     NNPLEPGSYRIWLNEVPIAKGNIRLDGVLVKSNARSLILYGIQG"
BASE COUNT          284 a          309 c          306 g          238 t
ORIGIN      
        1 tcaccctgaa tgccatacag aataagactc ctggcatttg acttgaccag aacgccgtca
       61 agccggatgt ttcctttggc tatgggaacc tcattgagcc agattcggta actgcccggc
      121 tcgagcggat tgttgccact aatctgaatc ggcggaaaga tcacacccag gtcgtagtag
      181 agactgttcc gaacgtcagt gagtttttcg acaaaactct ttccctcagc gttagccgtg
      241 ttgacaaacg gggtgagcgg ctcactcacc tcgaggacca agggaacagt aaccgtcagt
      301 tggggttcct ggccacggtc agccgttccc gtctgggcag tttcctcgat agcctgcatt
      361 tttcgggcac gaaacaaacc ataggctacg cttcccgtca cgcctgccag caggaaaaag
      421 ggaatcttcg gcaaacccgg gatgactgca agaacaagca agaggccgga agaaacggca
      481 attgccttcg gctgtgccag gatctgattc gccacgtctt tgccgagatt agagtcgctc
      541 gtttccgaag ccacccgcgt gatgaccatg ccagcggaaa tggaaatcag gagagccggg
      601 atttgggaaa cgaggccgtt tccgatggtc aggatcgagt aggtttggac agccttcatc
      661 agctccatgt tcttcatggc catgccaata atcaagccgc cgatgatatt gatcagagtg
      721 atgatgatgc ccgcaatggc atcgcctttg acaaacttca tcgctccgtc catggcgccg
      781 taaaactgcg attcacgact cagagcatcc cgccgttttt tcccctcctg gaaatcaata
      841 atgccagcgc ggatgtcggc atcaatgctc atctgcttgc cgggcatggc atccagcgtg
      901 aaccgggcag agacttcagc aacccgctct gcgcccttgg taatcacaat aaattgcacc
      961 aaggtaatga tcaaaaagat gacggcgcca accacgaaat tcccgctcac gacaaacatg
     1021 ccgaaggctc tgatgacttc gccggcgtcc gcttgcaaaa gaatgaggcg ggtggcggag
     1081 atgtccaacg agaggcgata aagagtcgta atcaggagca gcgtagggta ggaggcg
//
LOCUS       DASTWE010000200         1385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_684120, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000200 DASTWE010000000
VERSION     DASTWE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684120"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..136
                     /locus_tag="VFT30_02185"
                     /pseudo
     CDS             35..136
                     /locus_tag="VFT30_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            582..>1385
                     /locus_tag="VFT30_02190"
     CDS             582..>1385
                     /locus_tag="VFT30_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5091460.1"
                     /translation="MTLVPRYVPRDTSLAGLPQESRFFLVEPHAQLQGFCHWQDDRQG
                     CPTAVLVHGLEGCSESRYMRGIAAKAYRAGFNVIRMNQRTCGGTEHLSPTLYNSGLSG
                     DYRAILKELAHRDGLDHIWLIGYSMGGNLILKAGGEFGQTEPSLAGVAAVCPNINPTV
                     CARALEEPRNFIYHRHFLTRLKARLRRKAALLPGKWDLSKLDRISTISEFDDRYTAHD
                     GGYRDGADYYDRAGARHVLDTIAVPTLIITAQDDPFIPYSMFTVPKIQRH"
BASE COUNT          304 a          398 c          345 g          338 t
ORIGIN      
        1 tggcaaaata gccaccctta gccaggaagt tcccatgcgg caatcgaagt tcaccgagac
       61 ccagatcgtg tcgatcctga aggaggccga cgccgggtgc accgtcaacg agatctggcg
      121 gcagtacggc atcagccgcc acttacgccg gtatggacgt cgctggctcg tcgaacgctt
      181 ctttgcctgg ctccagtgga aacgacgtct ctggtgcgct gggaatacta cgccgcgaac
      241 ttctttgggt ttgtgcagct cgcctgcatc accatgctcc tcaaacaatt ttgagatgga
      301 ttctaatatt gccatctctc gttgtaattc atcctctccc ataacaacac ctcatgacat
      361 catcaatagc tgccgatagc tgtgagcatg atttctttat taaaatgacg agacagtttc
      421 tctttgtctt ttgtgggaaa ccttaaactc gagtcgggaa ctgttgtaag ctccttcaag
      481 ttatctgcat ggcgtacata ctcgatacta tgaccgatcc cgcaatgatt gatatcgtct
      541 cctcttttca gcctccgtta cctctccgga atgctcatct catgaccctc gtccctcgct
      601 acgtgccgcg ggacacgtcc ttggcaggat tgcctcagga atcgcgtttc tttctcgttg
      661 agccacatgc gcagcttcag ggattctgtc attggcaaga tgatcgccag gggtgcccaa
      721 cagcggtatt ggttcacggc cttgaaggct gcagcgaatc gcgatacatg cgcggcattg
      781 cggcaaaagc ctatcgcgca ggctttaatg ttatccgcat gaatcaacgt acctgcggcg
      841 gaaccgaaca tctctccccg accctttata acagcggctt gagcggagac taccgcgcga
      901 ttctcaagga actcgctcac agggacggac tcgatcatat ctggttgatt gggtactcca
      961 tgggcggaaa tctcatcttg aaagcgggcg gcgagtttgg ccaaaccgag ccctccttgg
     1021 caggtgtcgc ggcagtatgc ccgaacatca acccgactgt ttgtgctcgt gcattggaag
     1081 agcctcgcaa ttttatctac catcgccact ttctgacgcg attgaaagcg aggctgcgga
     1141 gaaaagcggc cctgctgcct gggaaatggg atctttccaa actcgatcgg atctccacga
     1201 tcagcgaatt tgacgatcgc tacaccgctc atgacggagg ctatcgagac ggcgccgatt
     1261 actacgaccg ggcgggcgca cgccatgttc tcgataccat cgcggttcct actctcatca
     1321 ttacggcaca agatgacccg tttatcccct actcgatgtt caccgtaccg aagattcagc
     1381 ggcat
//
LOCUS       DASTWE010000201         1254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_458017, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000201 DASTWE010000000
VERSION     DASTWE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458017"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFT30_02195"
     CDS             complement(<1..619)
                     /locus_tag="VFT30_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091461.1"
                     /translation="MNIEEATNLLWQSTQQNSVPTAALQKTLMMDDAYKVQLGILARR
                     QQAGEKLGGWKIGLSAAGARQAFGLSAPISAYLLAGRHFTNGHKFSHAAIGKPIIESE
                     LCFTIGKRLASPGVTREQVLASVSAVAPAFEVVDMRFNIGEDIPLGVADGIAQWGYVT
                     GTAISPYPTNLNLGEVMVEMKRNGEVFSKVVGKDVIDNQLDCIVWL"
     gene            784..>1254
                     /locus_tag="VFT30_02200"
     CDS             784..>1254
                     /locus_tag="VFT30_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5091462.1"
                     /translation="MRWFSQFVLSVWLLVGSPTFAQQQDFSKIDVKGSKVNGNVYILQ
                     ADRGSGNIGASVGPDGILIVDDQFAPLADKIRAALKEAGGGPLKFVLNTHWHFDHVGG
                     NKVFGPEAPIIAHTNVRKRLTTDQTMMGRAFPAEPKEAWPVITFDQSLSIHFNGE"
BASE COUNT          295 a          312 c          340 g          307 t
ORIGIN      
        1 caagccatac gatgcaatca agttgattgt cgatcacatc cttaccaaca accttggaga
       61 acacttcgcc gttgcgcttc atttcgacca ttacctcacc gagattcaaa ttcgttggat
      121 agggagaaat cgcagtacca gtcacatatc cccactgtgc gatgccatca gctacaccca
      181 gcggtatatc ctctccgatg ttgaagcgca tatcgaccac ttcaaacgct ggtgccacag
      241 cagagactga agcgagtact tgctctcgtg tcaccccggg gctagcaaga cgtttgccga
      301 tcgtgaagca cagctccgat tcgatgattg gcttgccgat tgcggcatgc gagaacttgt
      361 gaccattggt gaaatgccgg ccggcaagta gataagcact aatcggcgcg gacaagccga
      421 atgcttgccg agcccctgcg gctgatagtc cgattttcca accgccgagt ttttctccgg
      481 cttgttgtcg tcgagcgaga atcccgagtt ggactttgta ggcgtcgtcc atcattaacg
      541 tcttttgcaa cgcggctgtg ggtacactgt tctgctgagt ggattgccaa agtagattgg
      601 tggcttcttc gatattcata atgcgtcact cctttagtga agaacggtat agagcaagaa
      661 tgcagaggaa gacaagagag aggtccacaa tgatactgtg ggtctaatgc ccgatgatac
      721 ccctttgtgc atcagttact acccatgata gagcaacatg cagtgttcgt aggggagggg
      781 tttatgcgat ggttttctca gttcgtgctt agtgtttggc tgcttgtcgg atcaccgact
      841 ttcgcgcaac agcaagattt ctcgaagatt gatgtcaaag gcagcaaggt caatgggaac
      901 gtctatatat tgcaggctga tcggggaagc ggcaacatcg gcgcgtcggt cgggccggac
      961 ggtatcctga tcgtcgacga tcagttcgcg ccgcttgcgg acaagattcg cgcggcactc
     1021 aaagaagccg gcggcgggcc gctgaagttt gttcttaata cccattggca ttttgatcat
     1081 gtcggaggaa ataaggtatt tggtcccgaa gcgccaatca tcgctcatac gaacgtgcgt
     1141 aaacgtctca cgactgatca gacgatgatg gggcgggcgt tcccggcaga accgaaggaa
     1201 gcgtggccgg tgattacttt tgaccagtca ctctctatcc attttaacgg cgag
//
LOCUS       DASTWE010000202         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1201187, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000202 DASTWE010000000
VERSION     DASTWE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201187"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFT30_02205"
     CDS             complement(<1..462)
                     /locus_tag="VFT30_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU5091463.1"
                     /translation="MTTASHPHNRIERIGIDESGKGDYFGPLVIAAVFVDARTQGELK
                     LMEVRDSKKISDGRVLELAPDIRTVCPHSIIAIGPKKYNELYIKIKNLNRLLAWGHAK
                     ALENLLERGVTCERAISDQFGDERLILNALQEKGRKITLVQRTKAESDLAVA"
     gene            575..1447
                     /locus_tag="VFT30_02210"
     CDS             575..1447
                     /locus_tag="VFT30_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5091464.1"
                     /translation="MRMRIQRRIRATTIFCLLMGGVGLAGCETNPYTGRSQLLMTSVG
                     QEMQMGAQAYNQVKSDPKMRPSQDPREVEPVKRVAARIVEAAKRSKYAEMAQQFQWEV
                     TVIKDDKTANAFALPGGKMAVYTGIFPMAKTEAGLAAVMGHEVVHALARHGAERMSQG
                     QVANIGLQVVGAAVGISSKNPVLGQATMAALGVGTQVGVLLPFSRKHESEADYVGILL
                     AADAGYDPRESVALWERMAQVSGGGGQAEFLSTHPSHDTRIDQLKEWMPEAMAIYQRR
                     TAMPAAPLPEVGGR"
     gene            1537..1961
                     /gene="rnpB"
                     /locus_tag="VFT30_02215"
     ncRNA           1537..1961
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFT30_02215"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2195..>2555
                     /locus_tag="VFT30_02220"
     CDS             2195..>2555
                     /locus_tag="VFT30_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5091465.1"
                     /translation="MKLTGAEIFIECLKREGVKTVFALPGGVVLKIFDTLHQQKDIEV
                     ILTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPMVCFSGQV
                     PTSLIGNDAFQEADNMGL"
BASE COUNT          555 a          646 c          774 g          580 t
ORIGIN      
        1 ggcgacggcc aagtccgatt cggcttttgt cctctgtacc agcgtgatct tccgtccctt
       61 ctcttgcagt gcgttgagaa tcagtcgctc atcaccgaac tgatcggaaa tcgcccgctc
      121 gcaggtcacg cctcgttcca gtagattctc cagtgccttg gcatggcccc acgcgagcaa
      181 gcggttcaga ttttttatct tgatgtaaag ttcgttgtat ttcttcggtc cgatggcgat
      241 gatgctgtgt ggacagacgg tcctaatatc cggtgcaagt tccagcacgc ggccgtcgga
      301 aatcttcttg ctgtcacgaa cctccatcag tttcaattcg ccttgcgtcc tcgcatccac
      361 aaacacagcg gcaatgacga ggggtccaaa gtaatcgccc tttccggatt cgtcgatgcc
      421 gatgcgctcg atcctgttgt gaggatgtga cgcagtcgtc atgttcgtgc tctccctcta
      481 gaaatcccgt tgaagcggcg gtaatctatc aaccgattct aaagtggtca atcatgcaag
      541 gtttatcgag cgagactttg gctggaggtt gaagatgagg atgcgtatcc agaggcgcat
      601 acgcgcaacg acgatcttct gtcttctgat ggggggagtc ggcctcgcag gatgtgagac
      661 caatccctat acaggccggt cacagctgtt gatgacgtcg gtggggcaag aaatgcaaat
      721 gggggcccag gcgtacaatc aggtcaagag tgatcctaaa atgagacctt cccaagatcc
      781 gcgagaagtt gaaccggtga aacgggtagc ggctcggatc gtcgaggccg cgaaacggtc
      841 gaagtatgcc gagatggccc aacagtttca gtgggaagtc acggtcatca aggacgataa
      901 gacggcgaac gcttttgcgc tgcctggcgg gaagatggcg gtgtataccg gcattttccc
      961 catggccaag acggaagccg gcttagcggc cgtgatggga catgaagtgg tgcatgcatt
     1021 ggctcggcat ggcgcggagc gcatgagtca ggggcaagta gcgaatatcg ggcttcaagt
     1081 cgtcggtgca gcagtcggaa taagtagtaa gaatcctgtg cttggtcagg cgaccatggc
     1141 ggcgctcgga gtagggacgc aggtcggtgt cctgcttcct ttcagtcgaa aacatgaatc
     1201 ggaagccgac tacgtgggaa ttcttttggc cgcagatgcc gggtatgacc cgcgcgagtc
     1261 ggtcgcactc tgggaacgga tggcgcaggt atcgggcggt ggaggccagg cagaattctt
     1321 gtcgactcat ccgagccatg acaccaggat tgatcaattg aaagagtgga tgcctgaggc
     1381 tatggctatc tatcagagaa ggaccgccat gccggcggcc cctctaccgg aagtcggtgg
     1441 caggtaaggc tcttgcagat cgcggccaat cgtaataagc attgatccgt ctgcttgtta
     1501 ccgacccgca tcattcttta tactgaccca gcgtgggtgg aagactggat ggtcgctcgg
     1561 tgggttccca tcgagaggaa agtccggact ccatgggtag ggcgctgggt aactcccagg
     1621 cggagcaatc cgacaccggc accacagaaa tcaaaccgcc gatggccaag gcgatgggat
     1681 cccatctggt cgctgcggct caggtaaggg tgaaacggtg gggtaagagc ccaccgcggt
     1741 ggtggtgaca tcgccggcac ggtaagcgtc gcccggtgca aggccaaata ggaagacatg
     1801 tgcgggcttc ccatggagct ccgtgcaccg actggcccgg tcgaggtctt cgggtaggtc
     1861 gctagaggtt cgaggcaact cgaatcccag agaaatgatc atccaagcaa aggggaaact
     1921 tctttgcggg acagaatccg gcttataggt tttccatcca cgcccttcat tcggtgaaac
     1981 gctctccttt ccttgaggac ctcatttctt ctctcatcgg cctaccggga gtggttgtta
     2041 ttgaccgtcc tccgtgagaa tgctaggatc ttgaatcttc ccccttgatt tcaaacacat
     2101 ttctgcacga tgatgcgtgc ttcgagaggg gtgccgtttt aaggtctcac tatggacagt
     2161 agaccttatg tttgcggttg gatggagggg taccatgaaa ctcaccggtg ctgaaatctt
     2221 catcgaatgc ttgaagcggg aaggagtgaa gacggtcttc gcgcttccgg gcggagtcgt
     2281 cttgaagatt ttcgatacgc tccaccagca gaaggatatt gaagtgatct tgacacgtca
     2341 tgaacagggc gcgggtcata tggcggaagg atacgcgaag gcgaccggga aggccggcgt
     2401 gtgtctggtc acctccggcc ccggcatgac caatgtcatt acggccttag ccgatgccta
     2461 tatggattct gttccgatgg tctgtttcag cggccaagtc ccgaccagtt tgatcggaaa
     2521 cgatgctttt caggaggccg ataatatggg tttga
//
LOCUS       DASTWE010000203         1934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1072146, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000203 DASTWE010000000
VERSION     DASTWE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072146"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(590..976)
                     /locus_tag="VFT30_02225"
     CDS             complement(590..976)
                     /locus_tag="VFT30_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091466.1"
                     /translation="MKLGRCLHGFLLIALLLQGCAFSRGTLGDGFQSDAVATIQKGIT
                     TKEEVVHLLGAPDRLLPLNGRDVFQYYRYDAKAGSLLLLVLNFTRLSVKSDDLFVILN
                     RDGIVEDVISSKRTEGMEFRFWPFGE"
     gene            complement(987..1487)
                     /locus_tag="VFT30_02230"
     CDS             complement(987..1487)
                     /locus_tag="VFT30_02230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5091467.1"
                     /translation="MIERIHIRPAGQDDVETLVMFNAAMALETESRRLDLATLRKGTL
                     AFLGSPGYGFYILAELPEDKSYKPIGQLMITYEWSDWRNGAFWWIQSVYVVPDQRGLG
                     VFRAMHDHILVKAKADPRVCGIRLYVERENRRAQTVYQRVGLAPSVYTVFERDFVLGH
                     EAHQNR"
     gene            complement(1484..>1934)
                     /locus_tag="VFT30_02235"
     CDS             complement(1484..>1934)
                     /locus_tag="VFT30_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEU5091468.1"
                     /translation="VTLLIVVGLIGDHVIGQGLLVRLEHRIEQIPLVQSIYLTLKGMT
                     DILNFRSRFGRSKVVVFPFPRDGCWALGFVMGMAPPSLQVEPSRTLFMVFVPTAIHPF
                     TGFLAFIPEHALRPINLAVEDAMKMEFSAGFYKPQNGWLAPSASLPS"
BASE COUNT          487 a          510 c          527 g          410 t
ORIGIN      
        1 tctcgttgaa gaaaattgac gtcctgatcg gcagggatat atgaagagga gcgcacgcta
       61 ctgtagtggg acggatggtc ggatgtttct tgacccatgg ctcgccatta tagcggggct
      121 actagcaggc ggcaattcgc caggagtaga aacccttgat tgctggaatg gatagggata
      181 gaagggcggc ctgatgaggt gtcgctcaaa tccctggtgt attacaaccc acttcgggtc
      241 gcaccacact tcaaaggcgt caaccccaag tccggtacgg gaaaagatca aatccgggtc
      301 gatgggtgac catggtctta ccaaccagcc aaagttgaca cgagagtatt ttaagtcgag
      361 gaatcgatac gtcgccagaa ttcccgtatc agaatcggtg atcgagtcag gtgcccccag
      421 tttcgcgacc acatcagcca aggtggtctt gcccggtacg atgaaggcga cgtcctctgg
      481 agtgaggggg gcgttgagag agatccggac cacgttacag cccatccaag acaaacagac
      541 cagcactcct aacagaaagt gggaactctt tctccataaa ctgatcatgt cattccccga
      601 atggccagaa tcggaactcc atgccttctg tgcgtttgga tgagatgaca tcttcgacaa
      661 ttccatcccg gttgaggatg acgaataggt cgtcactctt gaccgaaaga cgtgtgaaat
      721 tgagaacgag cagcaagaga ctccctgctt tggcatcgta tcgataatac tgaaagacgt
      781 cccgaccgtt gagcggcagc agccgatcag gagctcccag gaggtgaacc acctcttcct
      841 tcgtcgtaat gcctttctga atggtcgcga cggcgtcgga ttggaatccg tcgcctagcg
      901 tcccccgact gaatgcgcat ccttgaagca acagggcgat cagtaagaag ccatgcaagc
      961 agcggccgag tttcatggga actcctttat ctattctggt gggcctcatg cccaaggacg
     1021 aaatcccgct cgaataccgt ataaactgac ggagcgagtc ctacacgttg atacacagtt
     1081 tgagccctgc gattctcccg ttccacatac aatctgatgc cgcacactcg tggatctgcc
     1141 ttggctttga cgaggatatg gtcatgcatc gctcggaaca cccccaagcc gcgctgatcc
     1201 ggtacgacat agacgctctg gatccaccaa aaggccccgt tccgccagtc gctccattcg
     1261 tacgtaatca tgagctggcc aatcggtttg tagcttttgt cttctggaag ttcggcaagg
     1321 atataaaatc catacccagg gcttcctaag aatgcaaggg tacccttgcg caaggtggca
     1381 agatcgagcc ggcgactctc tgtttcgagt gccatggcgg cattgaacat aacgagggtc
     1441 tccacatcgt cctgaccggc cggtctgatg tggatgcgct caatcatgag gggagcgatg
     1501 cggatggagc gagccatccg ttttgaggtt tatagaaccc ggctgaaaac tccattttca
     1561 tcgcgtcttc aaccgcaaga ttgatcgggc gcagcgcatg ttccgggatg aaggctagga
     1621 agccggtgaa ggggtgaatc gcggtcggga cgaaaaccat aaagagggtg cgggagggtt
     1681 cgacctgaag agacgggggc gccatcccca tcacaaaacc taacgcccag catccatccc
     1741 gaggaaaagg aaagaccact actttactac ggccaaagcg agatcggaag tttagaatat
     1801 cggtcatccc tttcaatgtg agatagatgc tctgcacgag cgggatctgc tcaatgcggt
     1861 gttccagtct caccaacaaa ccttgcccga tcacatgatc gccgataagg ccaacaacaa
     1921 tgagcagggt gacc
//
LOCUS       DASTWE010000204         1111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_134986, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000204 DASTWE010000000
VERSION     DASTWE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134986"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFT30_02240"
     CDS             complement(<1..887)
                     /locus_tag="VFT30_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091469.1"
                     /translation="MTQKTLDAKLARLHADPHSARDFILADAKDADMGAGLLAPGSTW
                     DGERERPRSLAEYRELMREVARQELVDILLMSASSHDLLGMEEHLFDSSPVTVAVRAN
                     DTTDIHLARGGCYPKALSAPFRSATIEQLQCGRVACSDEERQRGCNLGLYSVTLNNHP
                     EADLRTLEAYRAFRIEAEQKGFRHFLEVFDPNVTDAIEPERVGDFINDQIVRLLAAVP
                     RFGRPLFLKIAYHGPRCMEELAAYDPHLVPGVLGGAAGTTHDAFHLLSEARKYGARAA
                     LFGRKINRAEHQLTFIKFLR"
BASE COUNT          226 a          319 c          308 g          258 t
ORIGIN      
        1 cgcagaaact taatgaacgt gagctgatgt tcagcgcggt taatcttgcg tccaaacaga
       61 gctgctctcg cgccgtattt gcgtgcttct gacaagagat gaaaagcatc gtgcgttgtt
      121 ccggctgcac ccccaaggac gccgggcacg aggtgcggat cgtaagccgc cagttcttcc
      181 atgcacctcg gcccgtgata cgcgatcttc agaaataatg ggcgtccaaa tcttggaact
      241 gcggcgagca agcggacgat ttggtcgttg atgaaatccc cgacgcgctc cggctcgatg
      301 gcgtctgtaa catttgggtc gaaaacctcc aggaagtgac ggaacccttt ctgctcggct
      361 tcaatccgaa aagctcgata cgcttcgagc gtccggaggt cagcctctgg atggttgttc
      421 agcgtcactg aatacagccc caagttgcac ccgcgctggc gctcctcgtc gctgcaggct
      481 acacggccgc attgcaactg ttctatcgtc gcggatcgaa acggcgccga aagggccttc
      541 ggataacaac ctccgcgtgc caggtgaatg tccgtggtgt cattggcacg gaccgccacc
      601 gttaccggtg agctgtcgaa caagtgttct tccattccca ggagatcgtg gctgctggcc
      661 gacatcaaaa gaatatcgac cagttcctgg cgcgcgactt cccgcatcag ttcccgatat
      721 tcagccaggc tcctcggtcg ttcccgttcg ccatcccaag tcgacccggg tgccaataac
      781 cccgcaccca tgtcggcatc cttcgcgtcc gcgagaatga aatcccgtgc actgtgcgga
      841 tcggcgtgaa gtcgtgcgag ctttgcatcg agagtcttct gcgtcatgat cggtggggcg
      901 cacgcccgga ttgcttcatg atgcctccag gagatgttat caagttgcga atctcattcg
      961 ctggcttctt accatctctg gagggtttca gccaaccaaa tgcttcaacg cttgccggac
     1021 cttccaggca gatttgcctg gtttgggtgg gagttggttg aagtggaata tcgattctct
     1081 gagaaggctc tatgaagtcc aaatttcatg g
//
LOCUS       DASTWE010000205         1456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_167422, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000205 DASTWE010000000
VERSION     DASTWE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167422"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..634
                     /gene="gcvH"
                     /locus_tag="VFT30_02245"
     CDS             245..634
                     /gene="gcvH"
                     /locus_tag="VFT30_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5091470.1"
                     /translation="MIPSDLRFHKEHEWVRLDGKRATVGISDFAQDALGDIVFLDLPK
                     VGISVKVGQQIGEVESTKTTSTIYTPVSGTVSQINTALRDNPEVVNSDPYGKGWMAVI
                     ELADPAEIDQLMTASQYEAFLASQKKE"
     gene            792..1244
                     /locus_tag="VFT30_02250"
     CDS             792..1244
                     /locus_tag="VFT30_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU5091471.1"
                     /translation="MIKSLLLKLGMLSLTIGVVFWARWALQPPVQEVPAIEKQVVASQ
                     TTELEERESRSAGSSSRNVQGPNMREVTRTRPLKTAIHSRLDLNRASAGELESLPGIG
                     TVMAQRVIAFRESVGRFQKIEDLRGVKGIGAKKFERLKSFVMVSAANS"
     gene            1267..>1456
                     /locus_tag="VFT30_02255"
     CDS             1267..>1456
                     /locus_tag="VFT30_02255"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5091472.1"
                     /translation="MTNIREHLDLIQRGAVEVIHQVELEAKLKRSLAENRHLRIKAGF
                     DPTAPDLHLGHTVLIHKLK"
BASE COUNT          350 a          382 c          394 g          330 t
ORIGIN      
        1 ctcaaagctc tgctcagtac aattgaggag aacggcgctg atgctgttct tctgttttgt
       61 tttccggcgc ctctttggaa tcggaaccag ttcagattca atggcccaga tcgttccttg
      121 acaacgccag tctggcccac taccatccgg aagaatctcc gggttaaggg tgaggctgaa
      181 ccgtacgtta aactgccttc tttaacctca acttctagca ctatttttcc aaaatggagc
      241 cgacatgatt ccatccgatc tgcgatttca caaagagcat gagtgggttc gcctcgatgg
      301 gaaacgagcc actgtgggta tcagcgattt cgcgcaggat gccttgggcg atatcgtttt
      361 tctggatctg cccaaagtgg gcatctcggt caaggtcggc caacagatcg gagaagtcga
      421 atcgacgaaa acgacctcca cgatttatac gccggtgagc ggaacggtga gccagatcaa
      481 taccgccctt agagataatc ctgaagtggt aaattccgac ccttacggca aaggttggat
      541 ggccgtgatc gagcttgccg atcctgcgga aatcgatcaa ctgatgacgg catcgcagta
      601 cgaagccttc cttgccagcc agaagaaaga gtagtgtcgc tgtcgaggtt gaactttccg
      661 acacctgcct gcacgttcga ccgcatccgt cacctgtacc ttgacatggc atctccaacc
      721 cacatcgcaa ttctcggcgc cggtagctgc gatcccggca gctccattct tatttggaag
      781 agggcaggta catgatcaag tccctgcttc tgaaactcgg tatgctgtcc ttgacaatcg
      841 gtgtggtgtt ctgggcccga tgggctctac agccgccggt ccaagaggtc ccagcgatcg
      901 agaaacaggt cgtcgcctct cagactacgg aattagaaga gcgtgaaagc cggtctgctg
      961 gctcgtctag tcgaaacgta cagggtccaa acatgaggga ggtcacacgg acgaggccat
     1021 taaaaacggc gattcattca cgactcgacc tcaatcgtgc aagtgccggt gagctggagt
     1081 cgttgccagg gattggcacc gtgatggccc aacgtgtaat cgcttttcga gaatcagtcg
     1141 gacgatttca gaagatcgaa gaccttcgtg gggttaaagg gatcggtgcc aaaaaattcg
     1201 agcgcctcaa atccttcgta atggtctcgg cggcgaattc ctaacaaacg atggagcctc
     1261 aggattatga cgaacatacg cgagcaccta gacctgattc agcggggggc cgtcgaggtg
     1321 attcaccagg tcgaacttga agcgaagctc aagcggtcgc tcgcggagaa tcgccacttg
     1381 cgcatcaagg cgggatttga tccgacggcg ccggatcttc atctcggaca tacggttctg
     1441 attcataaat taaagc
//
LOCUS       DASTWE010000206         1460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1201313, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000206 DASTWE010000000
VERSION     DASTWE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201313"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..>1460)
                     /locus_tag="VFT30_02260"
     CDS             complement(282..>1460)
                     /locus_tag="VFT30_02260"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEU5091473.1"
                     /translation="ANPRQIIIVVNHTRSFIDIELVGGAGPRVRAKRKAQAEFLADLL
                     QDLQTADPDTPVISIGDYNAYQFNDGYTDPIATIKGTPTADEEMVVDASLDVVDPNFI
                     NLTDTHLSANQRYSFIFEGTPQAIDHFIINNAANSILQRYHIARNNSDFPEGPLFAND
                     ATRPERNSDHDMPVGYFKFPKAATTTVVSDASAGFSPTEQSVTLTANVTGPASISEGT
                     VTFTVRDSSNNVIGLPVVGNVSGGVATANYILPGGTAPQALTITGEFSGGDFTQPSSD
                     TATLSVSFNICLLYDPTKAVKSGAAYPVKIQLCDGNGNNVSSADIVVHAVNVQHVSTS
                     AFGDVLTAGNANEDNNFRFSDGTYILNLKTTGLSTGTYKLYFTAGDDPTLHSVEFQVK
                     "
BASE COUNT          311 a          363 c          439 g          347 t
ORIGIN      
        1 cagagcgggt cgggagttca gggcgaacat atgggtatcc ttccaaaacc aaagtgacgg
       61 atatcaattt ggagaggaga taccggtagg acaacagatc cggcaggggc ttaattcatg
      121 cttttaccag tgttcgaaag gcgggtcaat aacgaaaaaa gcctttgttt tgcagatgac
      181 tggattctgg tcaaggtgac aagaatcggt cacataggca gaggcagatc ggtcaaaaat
      241 ccgtgttccc gcggggcgca ggaacacgga tttccgttgg gttatttaac ctgaaactcc
      301 accgagtgga gcgtgggatc atctccggct gtgaagtaca gcttgtacgt tccggtgctg
      361 agtccggtgg tcttcaggtt gaggatgtaa gtgccatcac tgaacctgaa gttattgtcc
      421 tcgttcgcat ttccagcagt tagcacatcg ccaaaggcgg acgttgacac atgctggacg
      481 ttgaccgcat gaacaacgat gtctgctgac gaaacgttat tgccgttgcc gtcgcagagc
      541 tgaatcttga ccggataagc agccccactc ttcacggcct tggtcggatc gtaaagcaga
      601 cagatgttga acgacacaga caacgtcgcg gtatcgctgc tcggttgtgt gaagtcacca
      661 ccgctgaact caccggtgat cgtcagcgct tgcggcgcgg tgccgcctgg cagaatgtag
      721 tttgccgttg ccactccgcc cgacacgttt cctaccaccg ggagaccgat cacgttgtta
      781 ctcgagtctc tcacggtgaa ggtaactgtc ccttcgctga tcgacgctgg accagtgacg
      841 ttcgccgtca gcgtcacgct ctgctcggtc ggactgaagc cggccgaggc atcagagaca
      901 actgtcgtcg tcgccgcttt cgggaatttg aaatatccga ccggcatgtc gtggtcggag
      961 tttcgttccg gtctggtggc atcgttggcg aacaacggac cttcggggaa gtcggagttg
     1021 ttacgggcga tgtggtagcg ctggaggatt gaattcgcag cgttgttgat gatgaagtgg
     1081 tcgatggcct gtggcgttcc ttcgaagatg aagctgtaac gctggttggc cgacaagtgc
     1141 gtatccgtca ggttgatgaa gtttggatca acgacatcca gactcgcgtc gacaaccatt
     1201 tcttcgtcgg cagtcggggt gcccttgatg gtggcaatcg gatcggtgta gccgtcgttg
     1261 aattgatagg cgttgtagtc gccgatcgaa atcaccggcg tgtccgggtc cgccgtctgc
     1321 agatcctgaa gtagatcagc caggaactca gcttgcgctt tgcgtttcgc ccgaacgcgc
     1381 ggacctgcgc ctccaaccaa ctcgatatcg ataaacgatc ttgtgtggtt gacgacgatg
     1441 atgatctggc gtgggtttgc
//
LOCUS       DASTWE010000207         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1524542, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000207 DASTWE010000000
VERSION     DASTWE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524542"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFT30_02265"
     CDS             complement(<1..897)
                     /locus_tag="VFT30_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU5091474.1"
                     /translation="MASLGRGIEGSGERALVKRGVGLEAITIEQGPTLAKLPCWEAVQ
                     IPGGMFTASRAILTILLLFLIILEFVPWTQTIQVAGKVSAYTPYDRPQQIESRITGRV
                     KAWHIYEGVKVKKGDLVGELEDYDPTFMAPEILPLFEQRKVALEQTRQAALARADQLN
                     KRIGEMKKLVQAAVPSAEARVVEADNKVREAQQKVEQLKIDVITAQLNVDRHRQLVRD
                     GLVSQRELELTIQTEIGTKAGLQAAQAGLLAAEQSRSALSFGRDQITADVQQRLMDAI
                     ASRDAAVAEAAKATEQLADISYR"
     gene            complement(943..>2437)
                     /locus_tag="VFT30_02270"
     CDS             complement(943..>2437)
                     /locus_tag="VFT30_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5091475.1"
                     /translation="AVQPVMIWTLTSIMLGVLLFVGLFKTFHFYAVDVVQRRIFARVA
                     VAMVEQLPRNRFKGSQAALSNYFIETVFMQRALSTLLIDLLNVVVGGTVGMIVLVVYH
                     PYFLLYNLLLMAGFALTFFVLSRGALKTTLAMSHAKYDVFNWIQEISQNALQLKATDS
                     RPFLMQKTNDLVNRYVETRKARFAVLVRQYIGSVGGQAVAQSGALGLAAYLMAAGQLT
                     LGQLVAVQAVVGALVINFDSLIKNMGHVYYFFTSLTHLDEVFRQEQDYVSVESVVALP
                     KHLTQGVRVTCKGVSLIHNGASVFDGFNLDVAPGEKLGIYARTTGAKTALARVLGGLE
                     TPTNGVVQYNGVDLRYIDPNAINQCRSVMIDSQLSLIKGTIEENIVMGRSYVTYDDLN
                     WALRFTELEEDVEGLARGVKSDISTLGESLSPTHIVQILLARAILGRPQVLIFDGLIH
                     SLEPSLRERVLRRLCSKDEAWSVIFVSTDPNLTDHADRRIMLHHAHA"
BASE COUNT          586 a          679 c          629 g          543 t
ORIGIN      
        1 ccggtaagag atatctgcca actgctcggt tgccttggcg gcttccgcca ccgcagcgtc
       61 gcgcgatgct attgcgtcca tgagccgttg ctgtacatcc gcggtgattt gatcacgtcc
      121 gaagctcaag gctgaccgag actgctcagc agccaacaaa ccggcctgtg ctgcctgcaa
      181 acctgctttt gtgccgattt ccgtctgaat tgttaactca agttcacgtt gtgaaaccaa
      241 tccatccctg acaagttgcc gatgccgatc gacgttgagt tgcgctgtga tcacatcgat
      301 cttcagctgt tcgaccttct gttgtgcttc ccgcaccttg ttatcagctt ccaccactct
      361 ggcttcagcc gagggcacag cggcctgtac cagctttttc atctcaccaa ttcgtttatt
      421 tagctgatcc gccctagcta gagctgcctg acgtgtctgt tctagggcta ccttacgctg
      481 ttcgaagaga ggaaggatct cgggcgccat gaaggttggg tcgtagtcct ccaattcgcc
      541 gacgaggtca ccctttttga ccttgactcc ttcataaata tgccaggcct tgacacggcc
      601 tgtaatccgg gactcaatct gctgcgggcg atcgtaagga gtataggcgg aaacttttcc
      661 ggccacctgg atagtctgcg tccaagggac gaactcgaga atgatgagaa agagcaggag
      721 tatcgtcagt atcgcacggg atgccgtgaa catcccgcct ggaatctgta ccgcttccca
      781 gcatggcaac ttggccaatg tgggcccctg ttcgatagtg atcgcttcca agcccacccc
      841 tcgcttgacc agcgcccgtt ccccgctgcc ttcgataccg cgacctaggc tagccatgga
      901 gcctccatta atttaccggt gttgatgtgc cattccaagc gtttacgcat gagcatgatg
      961 caacataata cgacgatcgg catgatccgt aagattggga tccgtcgaca cgaagataac
     1021 cgaccatgct tcatctttag agcacaaccg acgcagcaca cgctcgcgca acgagggctc
     1081 gagcgagtga atcaagccgt caaatatgag gacttgagga cgtcccagaa tcgctctggc
     1141 caggaggatc tgcacaatat gagtcggcga aagggactct cccagcgtgg aaatatcgga
     1201 cttcacaccg cgtgccaagc cctcgacatc ttcttctagt tcggtgaaac ggagagccca
     1261 gttgaggtca tcataggtga cataggaacg ccccatgacg atattttctt cgatggttcc
     1321 cttgatcaac gacagttgag agtcgatcat gacactgcgg cattggttga tggcattggg
     1381 atcgatatac cggagatcca cgccattgta ctgaacgaca ccattggtcg gcgtttcgag
     1441 tccacccaac actctggcca aagcagtttt ggcccccgtc gtccgagcgt agataccgag
     1501 cttctcacct ggagcgacat ccaggttgaa gccgtcaaac acggacgctc cgttgtgaat
     1561 caaactgaca cctttgcagg tgacacggac cccttgagtc aaatgcttgg gcagggccac
     1621 aacggattcg actgaaacgt aatcctgctc ctgtctaaag acttcatcga gatgtgtcag
     1681 ggacgtgaag aaataataca cgtgtcccat gtttttgatg agagagtcga aattgatgac
     1741 gagtgctccg acgaccgcct gaacagcgac taactgccca agagtcaact gccctgctgc
     1801 catgaggtag gccgcgaggc caagagctcc ggattgtgcg accgcttgcc cgcccactga
     1861 cccaatatac tgccgcacga gaacggcaaa ccgggccttc cgggtttcga cgtatcgatt
     1921 gacgagatcg ttcgtcttct gcatgagaaa tgggcggctg tcggtggctt tcaactgcaa
     1981 cgcattctgc gaaatctctt gaatccaatt gaagacatcg tacttcgcat gagacatggc
     2041 gagcgtcgtc ttcagtgctc cacgagagag cacgaaaaac gtgagtgcaa agccggccat
     2101 gagcagaagg ttatacagga gaaaataggg atgatacaca accagcacga tcatgccgac
     2161 cgttccaccc actacgacgt tcagcagatc aatgagcagc gtggacaagg cacgttgcat
     2221 gaagaccgtt tcgataaagt agttggaaag cgcggcctgc gatcctttaa atcgattccg
     2281 aggaagctgt tcaaccatcg caacggcaac tcttgcaaag attcggcgtt gtacgacatc
     2341 cacggcgtag aagtggaatg tcttgaacag gccgacaaac aacagaaccc ccagcataat
     2401 gctggtcagg gtccaaatca tgaccggctg aaccgca
//
LOCUS       DASTWE010000208         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1427683, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000208 DASTWE010000000
VERSION     DASTWE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427683"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFT30_02275"
     CDS             complement(<1..563)
                     /locus_tag="VFT30_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091476.1"
                     /translation="MDQRANIIEQDIKDIVETRLEISRKIQLLDDKARYEMENMKMKL
                     SGLATDMAETGKEFIDRSSRTLNPIRQISARPGVVLAGVVLVGCVIGMLEKRFRRARV
                     YPYYPPKAHGAPVMPSEGEKESRETEAGVYPYFPEGHQESNRRRSSFLSDLWSDVKGN
                     VRTEVWRSKEAIEYALREFARDMTKEIV"
     gene            complement(527..949)
                     /locus_tag="VFT30_02280"
     CDS             complement(527..949)
                     /locus_tag="VFT30_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5091477.1"
                     /translation="MRNERDTHTTGNGIIGLLAGLIGDTRTLLRQELQLLRDEFFSEI
                     AKIRQAAVAIGVGIGFTVIGGLFALIMLVQVLHQFVHLPLWASYGLIGVILLAVGAAL
                     LIKAKHSLEDFNLMPRRTLRTMKEDAQWIKEQISLNKT"
BASE COUNT          308 a          374 c          289 g          324 t
ORIGIN      
        1 acgatttctt tggtcatatc ccgcgcaaat tcgcgaaggg catattcaat agcctccttt
       61 gaacgccaga cttcagtacg aacattgcct ttcacatcgc tccacaagtc cgacaaaaag
      121 gaggagcgac gtctatttga ctcctgatgc ccttctggaa aatacggata gacaccggcc
      181 tctgtttctc tgctttcctt ttctccctcc gacggcatca ccggggcccc atgtgctttg
      241 ggagggtaat agggatacac cctcgcgcga cgaaatctct tttccaacat tccaatcaca
      301 cacccgacca gcactacacc ggcaagtacc acccccggcc gcgcactgat ctggcggatt
      361 ggattcagcg tccgagagga gcggtcgatg aattctttcc ccgtctcagc catgtcggtg
      421 gcgagaccgg acaatttcat cttcatgttc tccatttcat accgtgcctt gtcatcgagc
      481 aactgtattt ttcggctgat ctcgagacga gtttcgacga tatcttttat gtcttgttca
      541 atgatatttg ctctttgatc cattgtgcgt cttccttcat tgtgcgtaaa gtccggcgcg
      601 gcatcaaatt gaaatcctcc aacgagtgct tggcctttat aagcagtgca gcaccgacgg
      661 cgagaagaat gaccccgatc agcccatagg atgcccacaa gggaagatga acgaactgat
      721 ggaggacctg gaccagcatg ataagcgcaa ataaacctcc gatgaccgtg aacccaattc
      781 ccactccgat cgcgacggct gcttggcgta tcttggcgat ctccgagaaa aactcatccc
      841 gcaaaagttg aagctcttgg cgaagcaatg tccgggtatc tccgatcagc ccggcgagca
      901 gcccgataat tccgttcccc gtcgtgtgcg tatctcgttc attcctcacg gcagcctccg
      961 acgacgagac aacagagcca actgaggctc cgatggtgag cagaatccac gggtaccatc
     1021 ggtaccgttg catgatcgtt ttcgcatcgc tgataagacc tctttcgcat caccgccaca
     1081 agcgacatga tctagaataa agaattcagt cacttgacac ctctttttct tcaatctgtt
     1141 tctctcattt tcggatcgta tcatgccgac tcgaacggac tactcggtaa cgccctagtc
     1201 actttatctt gtgatcgctg cttctaaaat gtagttgcta tgatcctcct acgagaaagc
     1261 ccacaggaca ggttgcaaag acttcagaga cccga
//
LOCUS       DASTWE010000209         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_878250, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000209 DASTWE010000000
VERSION     DASTWE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878250"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /gene="gltX"
                     /locus_tag="VFT30_02285"
     CDS             <1..1069
                     /gene="gltX"
                     /locus_tag="VFT30_02285"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5091478.1"
                     /translation="GLSPRYDGRCRHLRIAQPSGDAALRFKASQEGQTVIDDLIKGTV
                     VFDNAVMDDLIILRSNGYPTYNFSVVVDDALMGITHVVRGDDHLTNTPRQIPIFEALG
                     FAVPRFGHLPMILGADKTRLSKRHGATSIMAYKDMGYLPDAMVNYLVRLGWSHGDQEL
                     FSRQELIEKFSWNHVQKSAAVFNPDKLVWMNAEYIKTSSPRQVAEALVPLLEQAGLKK
                     DVEAVSDEWLAQLVVLVKERAKTLVEMVEWVRPYFGQTVTFDEEAAKKFLTSAIGPAL
                     SKLIARFESFPSFSRPEWENSFKQLVQEEGMKMGQLAQPVRVALTGRTASPGLFEVME
                     VLGRDRTLLRLRNGLERASRG"
BASE COUNT          260 a          281 c          330 g          261 t
ORIGIN      
        1 agggctgtcg ccacgctacg acggccgttg ccgccatttg aggatcgcac aaccgtccgg
       61 agatgccgcc cttcgattca aggcctcaca agaaggccag acggttatcg acgacctcat
      121 taagggaacg gtcgtcttcg acaacgcagt catggacgat ttgatcattc tcaggtccaa
      181 cgggtatccg acctacaact tttcagtcgt ggtcgatgat gcgttgatgg gtatcacaca
      241 cgtggtgcgg ggggacgacc atctgacgaa cacaccgcgg cagattccaa tttttgaagc
      301 cttggggttt gccgtgccgc gtttcggaca tttgccgatg attttaggag cagacaagac
      361 gcggctctct aagcgccatg gtgccacctc gatcatggct tataaagaca tgggctatct
      421 acccgacgcc atggtcaatt atctggtccg acttggctgg tctcacggag atcaagaact
      481 cttttcccga caggagctga tcgagaagtt ttcgtggaat cacgtgcaaa aatcggcggc
      541 cgtcttcaat ccagacaagt tagtctggat gaatgcggag tacattaaaa ccagttcccc
      601 ccgccaagtg gccgaagcac ttgtgccgct gctggagcaa gcggggttga agaaggatgt
      661 cgaagccgtc tcggatgaat ggcttgcaca attagtggtc ttggtgaagg agcgggccaa
      721 gacgttggtt gaaatggtgg agtgggtcag accgtatttc gggcaaactg ttacattcga
      781 tgaggaagcg gcaaagaagt ttctaacatc ggccattggt ccagctctca gcaagctcat
      841 tgcacggttt gaatccttcc cctcgttctc aagaccggaa tgggagaaca gcttcaaaca
      901 attggttcag gaagagggta tgaagatggg ccaacttgct cagccggtcc gcgttgcttt
      961 gactgggcga acggcaagtc cgggactttt tgaggtgatg gaagtgctgg gtcgtgaccg
     1021 gaccttgttg cgcctccgaa acgggctcga gcgcgcctcc aggggttgaa aggctctcgg
     1081 ccaaacccgc gtcgatcttg acggaattgt gattgatata ttactgtgaa cc
//
LOCUS       DASTWE010000210         1345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_878261, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000210 DASTWE010000000
VERSION     DASTWE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878261"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1345
                     /locus_tag="VFT30_02290"
     CDS             24..>1345
                     /locus_tag="VFT30_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091479.1"
                     /translation="MERYSKDSIPRSTLHVVIGERYDAAIPAAGGPQQMAGDYLYYSG
                     RASHFAEGSWGIFRVLDKADGTLQPLPGRNEIPQSAKSVCPSDAPVKTFNVAAIDKAI
                     RYNKGTPGVMEVDLERKMVFGNETGKMYVLEGEKTKVASGGLEPSPLTLHVNVGDCIK
                     VNLKNEMAKERAGFHVDNLAYDPKESMGINAGNNPGDQTVAPGQSRTYTYYAHPEFGE
                     NAALIQDWGNVIENPRNGLFGAVIIGPKGSQYRDPVSGEDLSQKSSWRADVIVDRTRP
                     ENQNRQNYRSFALLFQDEDNIIGTSFMPYIQKVAGVTAVNYRSEPTDYRTEKGCAYSE
                     VFACVKTGDQPVTPTIAAHVGDPVVIHVLGAFSEQVQLFSVSGHEWKHEPYLVGADLV
                     STMEFGGSEIINAWLHGGAGGPNGVAGDYLWLNQRPGYLDAGQWGMLKV"
BASE COUNT          310 a          353 c          429 g          253 t
ORIGIN      
        1 acatcagtgg gcactggttt cccatggaac gatatagcaa ggattcgatt ccgcggagca
       61 cgctgcatgt cgtgatcggt gaacgatacg acgcagccat acctgccgcg ggcggaccgc
      121 aacagatggc cggcgactac ctctactata gtggtcgcgc ttcccacttc gcggaaggca
      181 gctggggaat tttccgggtc ttggacaagg cggatggtac gttacagccc ctgccaggtc
      241 ggaacgagat tccacagtcg gctaagtcgg tctgtccatc cgacgctccg gtgaagacgt
      301 tcaacgtggc cgccatcgac aaggcgatcc gatacaacaa ggggacgcct ggcgtgatgg
      361 aagtcgatct ggagcgcaag atggtgtttg gtaatgaaac cggcaagatg tatgtgctcg
      421 aaggcgaaaa gacgaaagtg gcttcgggcg gtcttgagcc gagtccgctg acgctccacg
      481 tgaacgtcgg cgattgcatc aaggtcaatc tcaagaacga gatggccaag gagcgagccg
      541 ggttccatgt ggacaatctg gcgtacgatc cgaaagagtc catgggcatc aacgcaggga
      601 acaatcctgg cgatcaaacc gtcgcgccgg gacaaagccg gacgtacacg tactatgccc
      661 atcctgaatt cggcgaaaac gcggcgctga tccaggattg gggcaacgtg atcgagaatc
      721 cgcggaacgg actcttcggc gcggtcatca tcggaccgaa ggggtcgcag taccgcgatc
      781 cggtcagcgg cgaagacttg agtcaaaaga gttcttggag agcggacgtc atcgtcgatc
      841 ggaccaggcc ggagaaccaa aaccggcaga actaccggtc attcgcgttg ctcttccagg
      901 atgaagacaa tatcatcggg acgagcttca tgccgtacat ccagaaggtt gcaggggtga
      961 cggcggtcaa ctatcggtcg gagcccaccg attaccggac cgagaaaggc tgcgcttaca
     1021 gcgaggtgtt cgcctgcgtg aagacgggag accagccggt gactccgacc atcgcggcgc
     1081 atgtcggcga tccggtcgtc attcatgtac tgggagcttt cagtgagcag gttcaactgt
     1141 tcagcgtctc cggccatgaa tggaaacacg agccttattt ggtcggagca gatctcgtca
     1201 gcaccatgga gttcggcggg tccgaaatca tcaatgcctg gttgcatggt ggagctggtg
     1261 ggccgaacgg tgtggcaggt gactatctct ggctcaacca gcgacctgga tatctcgatg
     1321 ccgggcagtg gggaatgctg aaggt
//
LOCUS       DASTWE010000211         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1556960, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000211 DASTWE010000000
VERSION     DASTWE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556960"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFT30_02295"
     CDS             <1..189
                     /locus_tag="VFT30_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU5091480.1"
                     /translation="RMPGVPELMQTALDLGVRFIACTTTMGLMGITKNTLIDGIDQFA
                     GVTTYLAEAKQGSVNLFI"
     gene            217..462
                     /locus_tag="VFT30_02300"
     CDS             217..462
                     /locus_tag="VFT30_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU5091481.1"
                     /translation="MMQADVKLDTLGYFCPMPIILTSKKIKELALGQVLEVVSDDEGI
                     KKDMPAWCETTGHQMMGLEEEQAKSGPIYKAFVKKAK"
     gene            543..>1110
                     /gene="ftcD"
                     /locus_tag="VFT30_02305"
     CDS             543..>1110
                     /gene="ftcD"
                     /locus_tag="VFT30_02305"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEU5091482.1"
                     /translation="MDRLIECVPNFSEGRDQGILRALIDAVTSTAGVALLDHSTDADH
                     HRSVLTFCGGPDAIIEAAFRAVQIATELIDLRTHVGVHPRIGATDVVPFIPIRGTTMQ
                     DCVQLAKRLGERVGRELEIPVFLYERAAGRADHIPLEAVRRGGLEGLAFRMESDPGWI
                     PDFGPPRLHQSAGAIAIGARPPLIAYNVN"
BASE COUNT          254 a          292 c          324 g          240 t
ORIGIN      
        1 cgtatgcccg gtgtgcctga gttgatgcaa acggcccttg atctgggcgt gcgattcatc
       61 gcttgcacga cgacgatggg tctcatgggc attaccaaga atacattgat cgacggcatc
      121 gatcagttcg ccggcgtaac cacctatctg gcggaagcca aacagggcag tgtcaatttg
      181 ttcatttgaa ccgtccactg aagcgagggt gttttcatga tgcaggccga tgtcaaactc
      241 gacaccttgg ggtatttttg tccaatgccg atcattctca cgtccaagaa aatcaaagaa
      301 ttggcgttgg gacaggtatt ggaagtcgtc tccgatgatg agggcatcaa aaaagacatg
      361 ccggcttggt gtgaaaccac gggtcatcaa atgatgggct tggaagaaga acaagcaaag
      421 tcgggcccca tctataaggc gttcgttaag aaagcgaaat agccagactc ctgtaagaga
      481 gacggtgaag ccagaagccg gaggaacggt catcggtcct ccggcttttt cgttagcaac
      541 ctatggatcg gctcatcgag tgtgttccca acttcagtga aggtcgggat caaggaattc
      601 ttcgcgcctt gatcgatgcc gtgacgtcga cagccggcgt ggccctgcta gaccactcga
      661 cagatgcgga ccaccaccgg tcggtgctga cgttctgcgg tggcccggac gcgatcattg
      721 aagcggcttt tcgtgccgtc cagatcgcaa ctgaactcat cgacctgcgt acacacgtcg
      781 gagtacatcc ccgcatagga gcgacggatg ttgtgccgtt tatcccgatc aggggtacga
      841 caatgcagga ctgtgtccag cttgccaagc gactaggaga acgagtcgga cgtgaactgg
      901 agattcccgt tttcttatac gaacgtgctg cgggtcgtgc cgaccacatc ccattagagg
      961 cggtcagacg aggaggactc gagggcctgg ctttccggat ggagtcagac cctggttgga
     1021 ttcccgattt cggtcccccg cggctgcatc aaagcgcagg agcgattgcg atcggcgccc
     1081 ggcctccgct gatcgcctac aacgtgaacc
//
LOCUS       DASTWE010000212         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_329058, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000212 DASTWE010000000
VERSION     DASTWE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329058"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..966
                     /locus_tag="VFT30_02310"
     CDS             172..966
                     /locus_tag="VFT30_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5091483.1"
                     /translation="MDLQLSGRLALVTGSTGGIGLAIASALAGEGATVVINGRSKERV
                     TEAIGKIRQKHPHAKLEPLIADLSTVEAAKQAVSTFPHVDILINNLGSYEPKPFEAIS
                     DQDWQTIIETNFMSGVRLSRHYLPRMMTKNWGRIIFISSESAVNIPVEMIHYGVTKTM
                     QLALARGLAETTVGTAVTVNSVLAGPTKSEGVEKFVGDMAKSRGVEPSVVEREFFETV
                     RPSSLLKRFGTTDEVAALVTFVSSPLSSATNGAALRVDGGVVRSIL"
BASE COUNT          255 a          284 c          324 g          220 t
ORIGIN      
        1 cggcgtgagc gatcacacgg tgagccagag tctctatctg aaagatcccg acggaaacga
       61 agtggaacta tacgtcgatg cggacgaatc cgtatggaag gacaatccga cggcgagact
      121 gtctccgatc aagccgttgt agttatagct tcatacgaag aggagcaata catggattta
      181 caactatccg gcaggctcgc gctcgtgacc ggttcgaccg gggggatcgg gttggccatt
      241 gcttcggccc tggccggcga aggtgcgacg gtggtgatca atggccgatc gaaagaacga
      301 gtgactgagg cgatcggaaa gatccggcag aagcatcctc acgccaagct ggagccgctc
      361 attgccgatc tgagcacagt ggaggcggcg aagcaagccg tcagcacctt tccccacgtc
      421 gacatcttaa tcaataacct gggctcgtac gaacccaaac cgttcgaggc aatttccgat
      481 caggactggc agacgatcat cgaaaccaat tttatgagcg gtgtgcgctt gagccggcat
      541 tacttaccca gaatgatgac gaaaaactgg gggagaatca ttttcatctc gagcgaatcc
      601 gccgtcaata tcccggttga aatgatccac tacggcgtga cgaaaaccat gcagcttgcg
      661 ctggcgcgag gcctggcgga aaccacagtg ggcaccgccg tgacggtcaa ttcagtcttg
      721 gcgggaccga cgaagtctga aggcgtggag aaattcgttg gcgatatggc gaagtctcgc
      781 ggcgtcgaac cttctgtggt tgagagagag ttctttgaga ccgtccgtcc cagctccctg
      841 cttaagcggt ttggaaccac tgacgaagtg gcagcgctcg tgacgttcgt ctccagtccg
      901 ctgtcctctg cgaccaacgg cgcagccctc cgtgtggacg gcggcgtcgt gcgctcgatt
      961 ctctgatcag ggcgtaatga tgcaagaggt tcgatgcaat agacaagcaa catggggggt
     1021 cgatgacgca tcaactgatt cgattctcaa taggagttcg agctctagtt gtcgaccggt
     1081 cac
//
LOCUS       DASTWE010000213         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_296649, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000213 DASTWE010000000
VERSION     DASTWE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296649"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..841
                     /locus_tag="VFT30_02315"
     CDS             179..841
                     /locus_tag="VFT30_02315"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5091484.1"
                     /translation="MVSDYLLTMNRARISQQNQSSRASGHERQASLISAATSLFAANG
                     FTGTTTKQIAKAAGVSEALLFKHFPTKHALYTAILAEKAQYSELREAVEEATKKRDDE
                     RLFKLLASYRIRKGADPTMLRLLLFSALEGHELSDMFFQQQYRVFHDLLASYIRRRIE
                     DGAFRPVDPVLAAKAFFGVVLHHRLLHDIFGLPMHLSHEASVVEYVSLFLGGLVRKSP
                     DS"
     gene            858..>1148
                     /locus_tag="VFT30_02320"
     CDS             858..>1148
                     /locus_tag="VFT30_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5091485.1"
                     /translation="MSRLVRHPFLVLGIIIFLTITVLVVFRLSTGAKADTKKARLITV
                     GVVSPLRQDLDIHLSYTADISPNQVVNIFSRVDGYIAKLHVDKGDFVRANQIL"
BASE COUNT          274 a          323 c          260 g          291 t
ORIGIN      
        1 aactcgattc cgacttctct agtccgaccc tcccccttat attgtacgat tcttttcatg
       61 aacgagctta catgcccttt tagttcgagt gcttggcagg tcagagctga gaagatcact
      121 cgattccact ccattaatcg atcccacctt gttatccata caaacagtat gctaattcat
      181 ggtgagtgac tacttactca ccatgaatag agcaagaata tctcaacaga atcaatcgtc
      241 gcgagcctca ggtcatgagc gacaagccag cctgatcagc gctgcgacat cgttgtttgc
      301 cgcaaacggt tttaccggaa cgactactaa acagatcgcc aaggctgccg gtgtcagtga
      361 agcactgctc ttcaaacact ttccgaccaa gcacgcactg tatacggcaa ttcttgcaga
      421 aaaggctcaa tactccgagt tgcgagaagc cgtcgaagaa gcaaccaaga aacgagatga
      481 tgaacggctc tttaagttgc tcgccagcta tcggatcaga aagggcgcag atccgacgat
      541 gcttcgccta ctccttttta gtgccttgga aggacatgaa ctgtccgaca tgttttttca
      601 gcagcaatat cgagtctttc acgatctgct cgccagctac attcgacgac gcatcgagga
      661 tggcgctttc cgtccggtcg atccagtgct tgccgccaaa gccttcttcg gcgtcgtcct
      721 gcaccatcgg ctactgcacg atatcttcgg gttacccatg cacttgtctc acgaagcatc
      781 ggtagtcgaa tatgtttctc ttttcctcgg cgggctcgtc cgaaagtcgc cggactcgta
      841 gcctgacagg caccgccatg agtcgactcg tccgacatcc atttctagtc ctcgggatca
      901 tcatcttttt gacgatcacg gttcttgtag tctttcgcct gagcacgggt gccaaagccg
      961 acacgaagaa ggctcggctc attacggtcg gtgttgtgtc cccgctacga caggatctcg
     1021 atattcacct gtcctataca gcggacatct cacccaacca agtcgttaat atcttctcac
     1081 gggtcgacgg atacatcgca aagctgcatg tcgataaggg tgattttgtc agggcaaatc
     1141 agatactc
//
LOCUS       DASTWE010000214         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_975426, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000214 DASTWE010000000
VERSION     DASTWE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975426"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="lysA"
                     /locus_tag="VFT30_02325"
     CDS             <1..768
                     /gene="lysA"
                     /locus_tag="VFT30_02325"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5091486.1"
                     /translation="SKFGIASDRALEEYKMASSLSHTDVVGVHAHIGSQLTDVTPFVD
                     SLKKVVALIDTLKSQGINIRYLNIGGGLGITYSDEKPPLPQDLADAISPLVRDLGLTL
                     VMEPGRVIVGNAGILVTRALYEKAGETKHFVIVDAAMNDLIRPSLYGAYHEIRPVKEE
                     AVRRTRQTVDIVGPVCESGDFLAKDRSLPGIKPGELLAVMSAGAYGFVMASNYNSRPR
                     VPEVLVKGGEFHVIRERETYDDLIRGERIPSFLNETE"
     gene            777..>1070
                     /locus_tag="VFT30_02330"
     CDS             777..>1070
                     /locus_tag="VFT30_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5091487.1"
                     /translation="MFAGSLVAIITPFRQGKVDERALAELIEWQIANGTNGIVPCGTT
                     GESATLSHSEHNRVIELTVEVVRRRVPVIAGTGSNSTEEAIALTKHAKQAGADG"
BASE COUNT          241 a          280 c          319 g          230 t
ORIGIN      
        1 agcaagttcg gcattgcctc ggatcgcgcg cttgaagaat acaaaatggc atcctcactg
       61 agccataccg acgtcgtggg tgtccatgct cacatcggct cgcagttgac ggacgtgacg
      121 ccgttcgtcg attcactcaa gaaagtcgtg gcgctcatcg atacgttgaa gagccagggc
      181 atcaatatcc gctatctgaa catcggcggc gggctcggca tcacctattc cgatgagaag
      241 ccacctttgc cccaggacct ggctgatgcg atttcgccgc tcgttagaga tttagggctg
      301 acgctggtca tggaacctgg ccgcgtcatc gtcggcaacg ccggcatcct cgtcaccagg
      361 gcgctgtacg agaaggccgg agagacgaag cacttcgtca ttgtcgatgc cgctatgaac
      421 gatttgattc ggccaagcct atatggtgcc taccacgaaa ttcgccctgt gaaggaggaa
      481 gcagtccgtc ggaccagaca gacggttgat atcgttggac ccgtctgtga atcaggggat
      541 tttttggcca aggatcgatc gttgccgggc atcaagcccg gtgagttgtt ggcggtcatg
      601 agcgcgggag catatggctt tgtgatggca tccaattaca actctcggcc tcgtgtaccg
      661 gaggtactcg tcaagggcgg agagtttcac gtcattcgtg aacgagaaac atacgacgac
      721 ctcatacgag gcgagaggat tccgtcgttt ctcaatgaaa cggagtgaga gtgagtatgt
      781 ttgccggatc gcttgtcgct attatcacac cgttccgaca gggcaaagtc gacgaacgtg
      841 ctttggccga gttgatcgaa tggcagattg ccaatggtac caacggcatt gttccctgcg
      901 gaaccacggg tgaatcagcc acgctttctc atagcgagca taatcgggtg attgagttga
      961 cggtcgaagt ggttcgccga cgggtaccgg tcattgcagg aaccggttcc aacagcacgg
     1021 aagaagccat cgcgctcacg aaacacgcga agcaggctgg ggccgacggc
//
LOCUS       DASTWE010000215         1364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_587533, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000215 DASTWE010000000
VERSION     DASTWE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587533"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFT30_02335"
     CDS             complement(<1..203)
                     /locus_tag="VFT30_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091488.1"
                     /translation="MSEGRTSIPTHRSPADAIGQSVLPAQRNRPSSTPLVQALREARG
                     DVDTNSVNQVVQSAGETLDEQSR"
     gene            complement(357..710)
                     /locus_tag="VFT30_02340"
     CDS             complement(357..710)
                     /locus_tag="VFT30_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091489.1"
                     /translation="MGDMSDRSHGGALGDGSERNRMELYGSTVPVSSSQQVHVEIDEL
                     VLTGFTRSQGEEIADSLRETLAHLVAGDVAGWLGSESMNVEALDAGKVQVRRSGRPKS
                     TGELVARAIYGSLPK"
     gene            complement(698..>1364)
                     /locus_tag="VFT30_02345"
     CDS             complement(698..>1364)
                     /locus_tag="VFT30_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091490.1"
                     /translation="GSRGLGISALFAGASGTGKTMAAEVLAHTLRLDLYRIDLSQVIS
                     KYIGETEKNLRKVFDAAEGAAAILLFDEADALFGKRSEVKDSHDRYANVEVSYLLQRM
                     EAYRGLAILTTNMKGALDAAFLRRIRFVVQFPFPDPAQRAEIWRRTFPKAVPTEELDC
                     ERLARLNISGGHIRNIAMNATFLAADEGASVAMRHLLQAARAEYAKLEKPLTEAEIAR
                     WVT"
BASE COUNT          282 a          431 c          355 g          296 t
ORIGIN      
        1 tgccgggact gttcatcgag agtttcgcca gccgactgca cgacctgatt tacggaattt
       61 gtatcgacat caccacgagc ttctcgaagc gcctgaacca agggggtcga agatgggcgg
      121 ttacgctggg caggcaagac cgactgacca atcgcgtccg caggactcct atgcgtggga
      181 atcgaagtcc gtccttcact cacgacctac ctccatctcg tggttcatca catccggctt
      241 gaggctgagt acgcgctcga ccaatccacc gcagacccat tgacgctgta gccttaatga
      301 atgtcctgca cgtaatcggg ctgaaaccat cgtgtgtgtc gaggctgctc gcgtattcat
      361 ttgggcagac tcccgtagat cgcacgggca accagctctc cagtggattt gggacggccc
      421 gaccgacgca cctgaacctt gccggcatcg agagcctcca cgttcatcga ttccgaccca
      481 agccaacctg ccacatcacc ggcaaccagg tgcgccagag tttccctcaa cgaatccgcg
      541 atctcctcac cttgagaccg cgtgaatccg gttagaacca actcatcgat ctccacatga
      601 acttgttgag aggaacttac agggactgta gaaccataca gctccatcct attcctttca
      661 ctcccatcgc ccagcgctcc gccgtggctg cgatcgctca tgtcacccat cgcgcgatct
      721 ctgcttcggt cagtggcttt tccaacttgg cgtactcggc tcgtgctgct tgcaagagat
      781 gtctcatggc gacggaggct ccttcatccg ccgcgagaaa ggtcgcgttc atggcgatgt
      841 tgcgaatatg gccgccggaa atgttaagtc gcgccaaccg ctcgcaatcc agctcttccg
      901 tcggcacagc cttcgggaag gtgcgcctcc agatttctgc gcgctgggca gggtcaggga
      961 atggaaactg gaccacgaag cgaatccgcc gaagaaaggc cgcgtccaaa gctcctttca
     1021 tgttggtcgt caggatcgcc aaccctcgat acgcttccat gcgttgaagc agatagctca
     1081 cttccacatt ggcataccga tcgtgactgt ccttgacctc actccgctta ccgaagagcg
     1141 cgtccgcttc atcaaacagc agaatagcgg cggcgccttc tgccgcatcg aacaccttgc
     1201 gcaagttctt ttcagtctcg ccgatgtatt tactgatcac ttggctgaga tcgatgcggt
     1261 agagatcgag acgaagggtg tgcgccaata cttccgcagc catggtcttg ccggttccgc
     1321 ttgcgcctgc gaagagggcg ctgattccca gcccgcgcga tccc
//
LOCUS       DASTWE010000216         1248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1557060, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000216 DASTWE010000000
VERSION     DASTWE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557060"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFT30_02350"
     CDS             complement(<1..269)
                     /locus_tag="VFT30_02350"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091491.1"
                     /translation="MKARPSSKRTRSTKSPSTVRAKGSVNNRKISSKKAKYPDIRRDL
                     ERQRAAILSEVGEVLTHRGDLAAFPDVSDQASAEVDQNFSMRIRER"
     gene            complement(341..943)
                     /gene="recR"
                     /locus_tag="VFT30_02355"
     CDS             complement(341..943)
                     /gene="recR"
                     /locus_tag="VFT30_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU5091492.1"
                     /translation="MAVDQQGLLARLIKELVRLPGIGHKSAQRLAFHLMKAEREDALR
                     LADAIRAVKDGLTFCRQCRNIAEADLCEFCLDPKRDRTKIFVVEEPSTLYAVERAGAY
                     RGLYHVLLGALSPLDGVGPGDIKADELVDRVKLGGVEEIILATNPTIEGEATAIYLTR
                     LLKPFGVRVSRIAYGIPVGMDIEYADEVTLLKSIEGRRDL"
     gene            complement(946..>1248)
                     /locus_tag="VFT30_02360"
     CDS             complement(946..>1248)
                     /locus_tag="VFT30_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5091493.1"
                     /translation="MSNILKQAQAMQEQMAKIQEQAASKTASGTAGGGIVTVTANGAM
                     QIVSVTIDPEVVKSGDVDMLQDLVVAATNEALRKAKELMEGEMKALTGGMKIPGLL"
BASE COUNT          247 a          361 c          335 g          305 t
ORIGIN      
        1 cgctctcgaa tgcgcatgga aaaattctgg tcgacttcgg cggatgcttg gtcgctgacg
       61 tcgggaaagg ccgcgaggtc gcctcgatgt gtcaggactt ctccgacctc acttaggatt
      121 gcagcccgct ggcgttcaag atcacggcgg atgtcaggat atttcgcttt cttagaacta
      181 atcttccgat tgtttaccga acctttggcg cgaactgtgc tcggcgattt ggtcgaacga
      241 gtgcgtttac tggaagggcg tgctttcata gctatgttcc atgtgcgaga aacaggaaac
      301 tataccagcc tcggtgaggg gggtcaacga ggaccggaag ctacaaatca cgccgcccct
      361 cgatcgactt cagcaacgtt acttcgtccg cgtactcaat gtccatgccc actggaattc
      421 cataagcaat tcgggaaacg cgtacgccga agggcttgag caatcgggtc agataaatcg
      481 ctgtggcttc tccttcaatg gtggggttcg tcgcgagaat gatttcttca accccaccga
      541 gtttcacgcg atcaacaagt tcatcagctt tgatgtctcc cggacctacg ccgtcgagtg
      601 gggagagcgc tcccagcagg acgtgataga gtccgcggta ggcgcctgcc cgttcgacgg
      661 catacagcgt actcggttct tcaacgacga agatcttggt ccgatcgcgt ttcgggtcga
      721 gacaaaactc gcacaagtcc gcttcggcga tattgcgaca ttgtctgcaa aacgtcaacc
      781 catccttcac cgcacgaatg gcatccgcca gccgcaaggc atcctcacgc tcggccttca
      841 tgaggtgaaa ggccaaccgc tgggcgcttt tgtgaccgat tccggggaga cggactagtt
      901 ccttgatgag tctcgccagt aagccctgtt gatcgacggc catcgctaca atagacccgg
      961 aattttcatt ccaccggtca gcgctttcat ttcgccttcc atcaattcct tggctttgcg
     1021 gagcgcttcg ttcgtcgcgg ctaccaccag gtcctgcagc atatcgacat caccgctctt
     1081 gaccacctcg ggatcgattg tcacactgac aatctgcatc gccccgtttg ccgtgacggt
     1141 gacgatcccg ccgccggctg tcccgctggc ggtttttgag gcggcttgtt cctgaatctt
     1201 ggccatttgt tcctgcatgg cctgggcttg cttgagtatg ttgctcat
//
LOCUS       DASTWE010000217         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1233837, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000217 DASTWE010000000
VERSION     DASTWE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233837"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1033)
                     /locus_tag="VFT30_02365"
     CDS             complement(<1..>1033)
                     /locus_tag="VFT30_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEU5091494.1"
                     /translation="ANAYMEGTYSEVYPNMSQGEEGMKRLFKQFSFPGGIPSHVAPET
                     PGSIHEGGELGYAVSHAYGAAYDNPNLIVACVVGDGEAETGPLATSWHSNKFLNPLTD
                     GVVLPILHLNGYKIANPCVLARISHEELDQLFRGYGYTPYFVEGSDPEKMHQTMAATL
                     DTIIEDIHRIKTEARRTGAKKRPLWPMIILRSPKGWTCPKEIDGKRTEDYWRSHQVPM
                     GEMHEKAEHVKILEKWMKSYKPEELFDNTGRLKPDLADLPPKGTRRMSANPHANGGLL
                     LRDLRLPDFRDYGVQVTKPGAVSAESTRLMGKFLRDTMKLNMESRNFRLFSPDENNSN
                     RWQDAMEVTN"
BASE COUNT          186 a          298 c          298 g          251 t
ORIGIN      
        1 ggttggtgac ctccattgca tcttgccagc ggttcgaatt gttctcgtcc ggactgaaga
       61 ggcggaagtt tcggctctcc atgttcagct tcatcgtgtc gcgcaggaat tttcccatca
      121 atcgggtgga ttctgcggag acggcgccgg gcttcgtcac ctgcacgcca tagtcacgaa
      181 agtcgggaag ccgaagatct ctgagcaaga ggccaccgtt ggcatgtgga ttcgcgctca
      241 tgcggcgggt gcccttcggg ggcagatcgg cgaggtctgg ctttaatcgc ccggtattgt
      301 cgaacaactc ttccggcttg tagctcttca tccatttctc gagaattttg acgtgctccg
      361 ccttctcgtg catctccccc atcggcactt gatggctccg ccagtagtcc tcggtccgct
      421 tgccatcgat ttccttcggg caggtccatc ctttgggaga acggagaatg atcatcggcc
      481 acagcggtcg cttcttggca cccgtccgtc gcgcttcggt cttgatgcgg tgaatgtctt
      541 caatgatcgt gtcgagcgtt gccgccatcg tttggtgcat cttctcggga tcgcttccct
      601 cgacaaaata cggcgtgtag ccgtagcccc ggaagagctg gtccaattcc tcgtggctga
      661 tgcgggccag gacgcatggg ttagcaatct tgtacccgtt caaatgcagg atcggaagca
      721 ccacgccgtc ggtgagcgga ttgagaaact tgttcgagtg ccacgaggtg gccaatggac
      781 cggtttccgc ctctccgtcg ccgacgacgc aggcgacgat gagattggga ttgtcatacg
      841 ccgctccata ggcatgcgac acggcgtacc ccaattcacc cccctcatgg atggagcctg
      901 gagtttcagg agctacgtga ctggggatgc ctcccggaaa tgaaaactgc ttgaacaagc
      961 gcttcatgcc ctcttcgccc tgagacatgt tgggatacac ttccgagtag gtcccttcca
     1021 tataggcgtt ggc
//
LOCUS       DASTWE010000218         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_329146, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000218 DASTWE010000000
VERSION     DASTWE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329146"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFT30_02370"
     CDS             complement(<1..284)
                     /locus_tag="VFT30_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU5091495.1"
                     /translation="MDTTNVETQTPIISLTDAALKEVKRLINVQGIEEGGLRLGVKGG
                     GCSGLSYTINFDDKIGQYDQVHEIDGVKVIVDAKSAIYLQGTQLDYQKDM"
     gene            complement(307..705)
                     /gene="iscU"
                     /locus_tag="VFT30_02375"
     CDS             complement(307..705)
                     /gene="iscU"
                     /locus_tag="VFT30_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEU5091496.1"
                     /translation="MAYSDKVVDHFNNPRNMGSFKKDDADVGTGMVGAPECGDVMKLQ
                     IKVQNDTIVDAKFKTFGCGSAIASSSLATEWLKGKTIEEAQKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKADAQPAGTK"
     gene            complement(737..>1598)
                     /locus_tag="VFT30_02380"
     CDS             complement(737..>1598)
                     /locus_tag="VFT30_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5091497.1"
                     /translation="VTYLPVDKYGMVNPQDVQNAITDKTILISVMLANNEIGTINPIQ
                     EIGKIAKARGVLFHCDATQGVGKIPVDVQVMGIDLMSFSAHKIYGPKGIGALYVRKRN
                     PRVRIAAQMDGGGHERGMRSGTLPVPLIVGFGKACEICEQEMTAEAARLTKMRDRLQT
                     DIMAALEESYLNGHPTNRLPGNLNISFAYVEGESLLMGMKDIALSSGSACTSATLEPS
                     YVLRALGVGTELAHSSIRFGLGRFNSDDEIDYTIKKVIEVVTKLREMSPLYEMAKEGV
                     DLKSVQWAAH"
BASE COUNT          311 a          453 c          412 g          422 t
ORIGIN      
        1 atcatgtctt tttgataatc caattgggtt ccctggagat aaatggcgct cttagcatcg
       61 acgatcactt tgacaccatc gatctcatgg acttgatcgt actgtccgat cttgtcatcg
      121 aagttaatcg tatagctgag gcccgaacag cctcctccct ttactccgag gcgaagtccg
      181 ccttcctcaa tgccttgtac gttgatcagc cgcttgactt ctttcaaggc tgcgtcggtg
      241 agcgaaatga tcggagtctg agtctcgaca tttgttgtgt ccatgtgccg ctcccttcgt
      301 gggccgttac ttggtgccgg ccggctgcgc atcagccttc ttttgataat cggccaatgc
      361 tgctttgatg gcatcttccg cgaggaccga acagtgaatt ttcacaggag ggagattcag
      421 ctcctgtacg atgtccgtat tcttgatctt ctgggcttcc tcgatcgtct tccctttgag
      481 ccattcggta gccagactgg aactggcaat cgcggacccg cagccgaagg tcttgaactt
      541 ggcatcgacg atcgtatcgt tctgcacctt gatctgcagc ttcattacat caccgcactc
      601 cggggctccc accataccgg tgcccacgtc tgcatcgtcc ttcttaaagc ttcccatgtt
      661 gcggggatta ttgaaatgat cgacgacttt atcgctatat gccatggtgt cctccgaaat
      721 atacgttgta tcccagttag tgtgcagccc actgaacgga tttcaagtct acgccttcct
      781 tcgccatttc atagagtggg gacatttccc gcagcttggt cactacctca ataaccttct
      841 tgatcgtgta gtcaatctca tcatcgctgt tgaaacggcc caagccgaag cggatcgagg
      901 agtgggcgag ttccgtcccg acgccgagcg cccgcagcac ataggagggt tccagtgttg
      961 ccgaggtgca ggccgaaccg gacgagagcg cgatgtcctt catgcccatg agcagcgact
     1021 ccccctcgac gtaggcgaac gaaatattga gattgcccgg caaacggttt gtcgggtggc
     1081 cgttcaaata actttcttca agggcggcca taatgtcagt ttggaggcgg tctcgcatct
     1141 tggtcagccg cgccgcttcc gccgtcatct cctgctcaca gatttcacag gccttcccga
     1201 atcctacgat cagcggaacc ggcaaggtgc cggatcgcat gccgcgttca tggccccctc
     1261 cgtccatctg ggccgcgatt cgtacgcggg ggttccgctt tctgacgtag agcgcaccaa
     1321 tccctttggg tccatagatt ttgtgggctg agaacgacat gagatcgatg cccatgacct
     1381 ggacatcgac cggtattttc ccgacacctt gggtggcgtc gcaatggaac aagacccccc
     1441 ttgcttttgc aatcttaccg atttcctgga tgggattgat cgtgccgatt tcgttgttgg
     1501 ccagcatgac cgagatcagg atcgtcttgt cggtgatggc attctggacg tcttgtggat
     1561 tcaccatgcc gtatttatcg accggcaaat aggtgacg
//
LOCUS       DASTWE010000219         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1524711, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000219 DASTWE010000000
VERSION     DASTWE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524711"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFT30_02385"
     CDS             <1..242
                     /locus_tag="VFT30_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091498.1"
                     /translation="EDVQITPGEFISIAFAVWDGRKDAAGELVEKGSQKAVSSWWYFR
                     ADAPPDYSSYMYAAIAAALALGFQFVLIRKLKKGQ"
     gene            245..>1071
                     /locus_tag="VFT30_02390"
     CDS             245..>1071
                     /locus_tag="VFT30_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5091499.1"
                     /translation="MKAARLGVIGLVVGVVGGAALTTGGCANEQEKRGHELYTHYCSD
                     CHGPNGKQNEGFNWSAMPDPKPKDLSNKSEMGTFKDEELFSTISRDMLDTSEEGGDPI
                     GDDDFAVPTMPTFKYTLSEDEIWAIVGHVRTLHGMKMEFNVAARKTSLEEGLKAAQAK
                     FEQAKQAYEAAEKKASEEAELKSEQLKKDVDVDESAYAAEQAAMVQAKKEMDAAQVAL
                     NNFSTRPGKGVSIPRPDLTVKPAEVAKLVDRGKRLYENKYGCNGCHNLAGEGGKIGPA
                     "
BASE COUNT          293 a          234 c          331 g          213 t
ORIGIN      
        1 aagaagatgt tcaaattacg ccgggagaat tcatcagtat cgcctttgct gtatgggacg
       61 gacgaaaaga tgccgctggt gagttggtcg aaaagggatc tcaaaaagcc gtctcctctt
      121 ggtggtattt ccgagccgat gcaccgccgg actattccag ctatatgtac gcggcaatag
      181 ccgctgcatt ggccttggga tttcagtttg tcctgatcag aaaactcaag aaagggcagt
      241 gagcatgaag gcggcacggc tgggagtcat tggattggtg gttggtgtag tgggtggcgc
      301 ggccttgacc acaggagggt gcgcgaacga acaggaaaag cgggggcatg agctctatac
      361 ccactattgt agtgactgtc atggcccaaa cggcaagcaa aacgaagggt ttaattggtc
      421 cgctatgccg gatccgaaac cgaaagattt gtccaacaaa tcagaaatgg gcacgttcaa
      481 ggacgaagag ctcttcagta cgatctcacg cgacatgttg gacaccagcg aagagggtgg
      541 cgatccaatc ggagacgacg actttgcggt tccgacgatg ccgacattca aatatacgct
      601 gtctgaggat gagatttggg cgatcgtagg gcacgtgcgc acgttgcatg ggatgaagat
      661 ggagtttaat gtcgcggcac gcaagacctc gcttgaagaa gggttgaaag ccgcccaggc
      721 caagtttgaa caggccaagc aggcttatga agcggcagag aagaaagcca gcgaagaagc
      781 ggagctgaaa agtgagcagt tgaagaaaga tgtggacgtg gatgaatccg cctacgcggc
      841 tgaacaagcg gccatggttc aagccaagaa agagatggat gccgctcagg ttgcgttgaa
      901 caacttctcc accagaccgg gcaaaggcgt cagcattcca agacccgatt tgacggtcaa
      961 acccgcggaa gttgcaaaat tggtcgatcg tgggaaaaga ctctacgaga ataagtatgg
     1021 ttgcaatggc tgccacaatc ttgccggtga gggtggcaag attggtccag c
//
LOCUS       DASTWE010000220         1300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_425948, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000220 DASTWE010000000
VERSION     DASTWE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425948"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFT30_02395"
     CDS             <1..454
                     /locus_tag="VFT30_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091500.1"
                     /translation="YDQFLPTTESHPAALEPFRTIPSRGKPSLTDLGTWNVFANPDMP
                     GPQPKIRAILCDDQQSCPLSDAVLLDRAVARFKTPGLRDLGHSAPFMHNGQFDTLEQI
                     VEFYREVSDLQRAGRLRNGDEELGNIRLADQDVGPLAAFLKSLNEDYE"
     gene            complement(902..>1300)
                     /locus_tag="VFT30_02400"
     CDS             complement(902..>1300)
                     /locus_tag="VFT30_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU5091501.1"
                     /translation="YLLHAALCKNWGYERLHDQYSHLANEEVQHSAGLIDHILYLDGT
                     PEVGELDTIAQGRDVGALFRADLDFERDDVELLRKAIAHCAKVGDFTTRHLLEHMIED
                     SEEHVDWFEIRLKTINQVGLDRFLSEQIKK"
BASE COUNT          293 a          385 c          334 g          288 t
ORIGIN      
        1 ctacgatcag ttccttccaa ccaccgaatc ccatcctgca gcgttggaac cattccggac
       61 catcccgagc agagggaaac cgtccctgac agatctcggc acatggaacg tgtttgccaa
      121 tccggatatg ccgggccctc agccgaaaat ccgcgctatt ctgtgtgacg atcagcagtc
      181 ctgtccgcta tcggatgctg tgctgctcga ccgtgccgtt gcgcgcttca agacaccagg
      241 cctccgggac cttggccatt cggccccctt catgcacaac ggtcaattcg acacgcttga
      301 gcaaatcgtg gagttctatc gggaggtctc ggatctccaa cgggcaggtc gtcttcgaaa
      361 cggagatgag gagctcggga acattcgtct ggccgatcaa gacgtcggtc cgctggcggc
      421 gttcctcaag tcactgaatg aggactatga ataaacgact gccggctata ccgatgggaa
      481 atatcgctcg ctctcagcag gctggttata agagagacgc gccctgcccc gagattccac
      541 tggtgctctg ccggcgctgc cacagaccga cggggcatcg gaatcccctg cgctggtgcg
      601 tcaagtgtcg aatgttccag catcaacgag ggcaagccat agggctggca agatccctca
      661 cgacgcggcg gctcaaagca tggctaaaaa cttttccaca attatagatc cgatatcgat
      721 ccgagaagcc catgacgtcg gaagtcatgc aaggtcaggc tcacgtgttc gcttggtgct
      781 tgccttctcg gaagcacctt cagcaatggc atctcggtat gtgactgaag taatgaaacc
      841 cgttgcccgc ctgtgcggag aagcgggact cgactatgcg ccgaccaggc tggcggactc
      901 cttatttctt aatctgctcc gaaaggaacc gatcgagccc gacttggttg attgtcttca
      961 ggcgaatctc aaaccagtcg acgtgctcct ctgaatcttc aatcatatgc tccaacagat
     1021 gacgggttgt aaaatcgccg actttggcac aatgtgcgat ggccttacga agcaattcaa
     1081 cgtcgtcgcg ttcaaaatcc agatccgctc gaaacagagc ccctacatcc cgcccctgtg
     1141 ctatcgtatc aagttctccc acctccggcg taccgtctag gtacaagata tggtctatta
     1201 gcccggcaga gtgttgcact tcttcgttcg ccagatggct atactgatca tgcagtcgtt
     1261 cgtatcccca attcttacac agagccgcat gcagcagata
//
LOCUS       DASTWE010000221         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_232338, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000221 DASTWE010000000
VERSION     DASTWE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232338"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..560
                     /locus_tag="VFT30_02405"
     CDS             93..560
                     /locus_tag="VFT30_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091502.1"
                     /translation="MANKKKKTVAKKSSRTKKATPSPKKKAVKKTVKKRAAVKASKPA
                     SKKKITKKTGKKPVKKTPPKASPPSTSAKKVAGEEEDVTPRKPVAAKPAIPMEPDEAD
                     LDDEDEIVSDELEIEGEIEEDDVQEELDLDADDDGDELIEKSEDLLDDDYRHN"
     gene            804..>1583
                     /locus_tag="VFT30_02410"
     CDS             804..>1583
                     /locus_tag="VFT30_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU5091503.1"
                     /translation="MALVLVSAIVTCPPQADAGCIAEALATGIEAGLVIHLSPSCTPA
                     ERETHAVRGEAVMDAVAKGRPVDLLGVIVHGDLIFDRLAVQTTSRSRVPEIANQADRA
                     GKNGLRVVRETLSLRDSVVAGAVRHRSAEDTLWFEKPVNFNGSHFKEGVDLSRSVFQA
                     PVELSAATFDKEAYFVQGQFAQQMDCRETKFGPSTRFHRSVFRGPVNCTGALFDGMAE
                     FLEVKFEQPVVFERARFGLGTGFSGSRFKSRVSFGEAIFSRE"
BASE COUNT          396 a          387 c          468 g          332 t
ORIGIN      
        1 gatggaaatc aagatcgggt ggttgcaaga ggctgttcgt tcatgctaga tggaggccgc
       61 gcgccacgct gaaacgaaga ggaggaatcg gaatggcgaa caagaagaaa aaaaccgtag
      121 cgaaaaaatc gtcgcggacg aaaaaagcta caccgtcgcc gaagaagaag gcggtcaaga
      181 aaacggtgaa aaagcgtgcg gccgtcaagg cgtccaaacc tgcctcgaag aaaaagatca
      241 ccaagaaaac tggcaaaaaa cctgtaaaga agaccccacc taaggccagc ccacccagca
      301 catccgctaa gaaagtcgca ggggaagaag aagacgtgac tccacgcaag cccgtggcgg
      361 ctaaaccggc catacccatg gaaccggacg aggccgatct cgatgacgag gatgagattg
      421 tcagcgatga actggaaata gagggcgaga tcgaagaaga cgacgtccaa gaagaattgg
      481 atctcgacgc cgatgatgac ggagacgagc ttatcgagaa gtccgaagat ttgttggacg
      541 acgattaccg acataactga tgtcccgttc ttctacgatc gtcccggctc cggctctagt
      601 accgttcgat gcaactcatc ataagaagca agggacgtgt aggtctgcgt tcatcgcttc
      661 tacgcaccgc cgctgaaacc aattagctca agcggcggtg tctcatattg catcccaaac
      721 attgcgttgc gctccccatg caatatggac agaaccttgc ttcaccgcag cttcttcgga
      781 agcagatgtg gtttggttgg tatgtggctc tggtgcttgt aagcgccatt gtgacttgtc
      841 caccccaagc ggatgctggt tgcattgccg aggcactcgc gactggaatt gaagcgggcc
      901 ttgtgatcca tctttcgccc agctgtacac cggctgagcg ggaaactcat gcagtgcgtg
      961 gcgaagccgt tatggatgcg gtcgccaagg gacgtcccgt cgatctcctt ggtgtgatcg
     1021 tgcacgggga tcttatcttc gatcgtcttg ctgtacaaac aacgtcacgt tcacgggtac
     1081 cggagatagc gaatcaagcg gatcgtgccg gcaagaacgg gctacgagtt gttcgcgaaa
     1141 cactgagcct aagggattct gttgtggcag gtgcagtgcg ccatcggtca gccgaggaca
     1201 cgctttggtt tgaaaagcct gtcaacttta acgggtctca tttcaaagag ggtgtggatc
     1261 tctcgcggtc ggtctttcaa gcaccagtcg aactttccgc tgccacattt gataaagaag
     1321 cctatttcgt gcagggacag ttcgctcagc agatggattg tcgggagacc aagtttggcc
     1381 ccagcactag gtttcatcgg tccgtatttc gtggaccggt gaattgtacc ggagcactct
     1441 tcgacggcat ggcggagttc cttgaagtga agtttgagca gccggtggtg tttgagcggg
     1501 ccaggttcgg tctggggacc ggattttccg gaagtcggtt caagagtcgg gtcagcttcg
     1561 gtgaagcgat ttttagccgg gag
//
LOCUS       DASTWE010000222         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_781641, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000222 DASTWE010000000
VERSION     DASTWE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781641"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFT30_02415"
     CDS             complement(<1..645)
                     /locus_tag="VFT30_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5091504.1"
                     /translation="MNTEPTTSPTVSEIERDVEARSAVLKKELGLFDLVLTQIMFVVG
                     TFWVGWAARLGYEQNVFWILAIVTFYLPLAAAVIFLNRLMPLEGGLYQWSKLAFNDFV
                     GFLVGWNLWVFAITILASAGLLVTTAISYSIGPRAAWIKADPKMITLISFLLVIAMML
                     AAMRGLALGKWVHNTGGLVHLITFGTLILLPLFALSRATGAEYRPFAIQFPTFSE"
     gene            complement(767..>1041)
                     /locus_tag="VFT30_02420"
     CDS             complement(767..>1041)
                     /locus_tag="VFT30_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091505.1"
                     /translation="THPERLRGVIGICGGLPGDWETSSTYRNAEIDVMHLAGTRDEFY
                     TPERVRDYERQLKTRARSVTFKSYDAAHEIVPEMRSDVVQWLTKLG"
BASE COUNT          257 a          273 c          297 g          214 t
ORIGIN      
        1 ctcggagaag gtcggaaact gaatagcaaa gggccggtac tcggctcccg tagcccgtga
       61 aagagcaaag agcggcaaca ggatcaaggt gccgaaagtg attaagtgca cgagtccgcc
      121 ggtattgtgg acccatttgc ccagagcgag accacgcatc gccgccagca tcatagcgat
      181 gacaagcaga aaacttatca gcgtgatcat cttcggatca gctttgatcc acgctgccct
      241 tggtccaata gaatacgaga tggccgtagt cactaatagt ccggcgctgg ccagaatagt
      301 gatggcaaag acccagaggt tccaacccac gaggaatccg acaaagtcat tgaaagcaag
      361 cttggaccac tggtaaagac cgccttcgag gggcataagg cgattgagaa agatcacggc
      421 agcggcgagc gggagataga acgtgacgat ggcgaggatc cagaatacat tttgctcgta
      481 ccctaatctc gcggcccaac ctacccagaa cgttccgacg acgaacatga tctgagtcaa
      541 caccagatcg aaaaggccga gctccttttt taagacggca ctgcgggcct caacatcacg
      601 ctcgatctca gagacggttg gcgaggtagt gggttcagtg ttcattgatg atgttaagag
      661 tcagagatct ctgttggata cagtcagtac cgtccgcggt agcggatggg tgcgtagcgg
      721 tgcgcgatgg ctgcgcaccc acccgctatc gcaggtggta ctgacctcat ccaagcttgg
      781 tcaaccattg gaccacatcg gagcgcattt cgggaacgat ttcgtgcgcg gcgtcatatg
      841 atttgaaggt gacggaccgc gcgcgtgtct tcagttgtcg ctcgtaatct cgcacacgtt
      901 cgggtgtgta gaattcgtcg cgcgtgccgg cgagatgcat cacgtctatt tctgcgttgc
      961 gatatgttga gcttgtttcc cagtcgccgg gcaggccgcc acaaatacct ataactccac
     1021 gcaaccgctc cggatgtgta a
//
LOCUS       DASTWE010000223         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1137031, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000223 DASTWE010000000
VERSION     DASTWE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137031"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..742
                     /locus_tag="VFT30_02425"
     CDS             125..742
                     /locus_tag="VFT30_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091506.1"
                     /translation="MTKNQEPYEEGKRAGAHPLLVVFGILIGLWLFVALIVPSSRNKQ
                     ATGTEGPNVSVIEDPEAAPVMFKVQTTILDMNTLGLVVPSQATDSQIVALLKRLKQAR
                     LDGALGEQLPSTTPGHKLGDHAIADIFIFSDKQHAEADTIRTLAQGAHAPGTLYPNAV
                     PFEVAMEQVRGHYRIDLNDTGSPDKGSLGFADESGVHSKHYKRIF"
     gene            complement(773..>1084)
                     /locus_tag="VFT30_02430"
     CDS             complement(773..>1084)
                     /locus_tag="VFT30_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091507.1"
                     /translation="WATPHGVRVKVSIPDELEVFKRRLFGVVVGSPGQQLMMSNLLSR
                     TIADWKMRVAEEEGMLTERRDTMLAPFIGPLAHLFPKLETLFEADQSAMREAVRRRVG
                     I"
BASE COUNT          264 a          329 c          274 g          217 t
ORIGIN      
        1 tggaaaaagg acggtcgcta acagagatga cgttggccga aatggatgag ctgtgggacg
       61 aagccaagca acggttacag agaccgcagc cgaacaggac accgcacatc ggagagcacg
      121 ctccatgacg aaaaaccaag aaccttacga agagggaaaa cgtgccgggg cccatccgct
      181 gcttgtggta tttggcatcc tcatcggact atggttgttc gtcgctctga tcgtccccag
      241 ctcaagaaac aaacaggcga ccggtaccga aggccccaac gtctcggtca ttgaagatcc
      301 ggaagccgcg cctgtcatgt tcaaggtcca gacgaccatt ctggatatga ataccctggg
      361 gttggtcgtc ccctctcaag cgacggatag tcagatcgtc gccctcttga aacgcctcaa
      421 acaagcgcgc ttggacggcg cactcggtga acagcttcct tcaaccacac caggccacaa
      481 actcggcgat cacgccatcg ccgacatttt catcttttca gacaaacaac acgctgaagc
      541 ggataccatc cgtacgctgg cgcaaggagc ccatgcgccc gggacgctct acccgaatgc
      601 tgttccgttt gaggtggcca tggagcaagt tcgtggccac taccgtatcg acttgaatga
      661 taccggcagc ccggacaagg gatcgctggg tttcgcagac gaatcaggtg tccattcgaa
      721 acactacaag agaatctttt gacggatcgg cctgcttttt ttccgcagaa agtcagatgc
      781 cgacgcgtct tctcactgct tctcgcatag ccgattgatc cgcttcaaat aacgtctcca
      841 actttgggaa cagatgggcc agcggcccga taaatggcgc cagcatcgta tcacgtcgtt
      901 ctgtcagcat accctcctct tcggccaccc tcatcttcca gtcggcaatc gttctggaga
      961 gtaggttgct catcatcaat tgctggcccg gcgagccgac aaccaccccg aagagacgac
     1021 gcttaaacac ctccagctca tccggtattg aaaccttcac ccgcacacca tgtggcgtcg
     1081 ccca
//
LOCUS       DASTWE010000224         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_103112, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000224 DASTWE010000000
VERSION     DASTWE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103112"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..495)
                     /gene="greA"
                     /locus_tag="VFT30_02435"
     CDS             complement(19..495)
                     /gene="greA"
                     /locus_tag="VFT30_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5091508.1"
                     /translation="MPTPITKKGYDALKAELDRLRKIERPKVIEAIAEARAHGDLSEN
                     AEYDAAKERQGFIESRIAELETKLADARVVETAGRTSETVVFGATVSVVEQESQAKKQ
                     YTLVGQDEADMKFNKISVQSPVGRALIGKRVGDFVEVKTPVKLVEYEVVEIKFEEF"
     gene            complement(640..>2064)
                     /gene="carB"
                     /locus_tag="VFT30_02440"
     CDS             complement(640..>2064)
                     /gene="carB"
                     /locus_tag="VFT30_02440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5091509.1"
                     /translation="NCNPETVSTDYDTSDRLYFEPLTHEDVLNIVHREQPIGVVLQFG
                     GQTPLKLALPLSKAGVRILGTSPDAIDLAEDRERFRDLLNRLGLKQAESGSARSIEEA
                     VQIARRISYPVMVRPSYVLGGRSMQIVYDEAGLLEYMHSAVKASPNHPVLIDRYLADA
                     IEVDADAISDGETVVVAGIMEHIEEAGVHSGDSACSLPPYTLDKPIVRDIERQMRLLA
                     LELGVVGLMNAQFAVKGRTIYVLEVNPRGSRTVPFVSKAIGIPLAKLAMKVMMGKTLK
                     ELGFTDAPLPAHFSVKEAVFPFNKFPGVDVLLGPEMKSTGEVMGIDGDFGWAFAKSQA
                     GAGAVLPTSGTAFISVKESDRPAALEVGRVLNKLGIRIQGTSGTAGYLRDHGLSVEVV
                     NKVAEGRPHIVDHIKNGSIALVVNTVRTASAHLDSLSIRREALHRGIPYFTTMRGAHA
                     AAMGIEAILKKDLAIRTLQEYHRT"
BASE COUNT          428 a          643 c          504 g          489 t
ORIGIN      
        1 gggcgtgcat ccggcacgtc agaattcctc gaatttgatc tccacaacct cgtattcgac
       61 caactttacg ggagtcttca cttccacgaa atctccgaca cgcttaccga tgagcgcacg
      121 accgacaggg gactgtaccg agatcttgtt gaatttcatg tcggcttcgt cctgcccgac
      181 caaggtgtat tgttttttcg cttgcgattc ttgctcgacg actgacaccg tcgcgccgaa
      241 aacgaccgtc tcgctggtgc gtcccgcggt ttccactaca cgggcatctg cgagtttcgt
      301 ctcgagctcg gcgatacgtg actcaataaa cccttggcgc tctttggcgg cgtcatactc
      361 ggcgttctca ctgagatcgc cgtgtgctct ggcctccgcg atcgcttcga tcactttcgg
      421 ccgctcaatc ttacgcaaac gatccaattc cgcctttaac gcatcatagc ccttcttcgt
      481 aatgggggtc ggcatgctct ggtcgctcct gagaggatgc tgcaaaagcc ctccagcttc
      541 gttctcgcat cgttcaaggc ctcaacgtac caactacgta cgcctcagcc gttcactcgc
      601 tgcggccttg ctggaagagt tttttcagca tcctctaatt taggtccgat gatactcttg
      661 cagcgtgcga atcgctaagt cctttttcaa gatcgcttcg atgcccatcg ccgccgcatg
      721 ggctcctctc atcgtcgtga aatacggaat gcctcgatga agggcttctc gtcggatgga
      781 taatgaatcc agatgagccg aggcggtccg caccgtattc accacgaggg ctatagaacc
      841 gttcttgata tggtcgacaa tgtgcggtct gccctcggcc accttattga cgacttctac
      901 ggaaagaccg tgatcacgca aatatcccgc tgtcccgctc gttccctgaa tacgtattcc
      961 cagcttgttc agcactcgcc ccacttcaag agcggcaggt cgatcggatt ctttcacgct
     1021 gataaaggct gttcccgacg tcggcagcac cgctccggct cccgcttggg actttgcaaa
     1081 tgcccatccg aaatctccgt cgatgcccat cacttctccg gtcgatttca tttcaggccc
     1141 cagcagcaca tcgacccccg ggaatttatt gaacggaaag actgcttctt tgaccgagaa
     1201 atgtgccggc agcggagcat ccgtaaagcc aagttccttc agtgtttttc ccatcatcac
     1261 cttcatcgcc aacttggcga gcggtatgcc gatcgctttg ctcacgaacg gcacggttcg
     1321 agacccgcga gggttgactt caagcacata gatggtccgg cctttgacgg cgaactgcgc
     1381 attcataagc ccaacgacgc caagctccaa tgccagcaaa cgcatttgcc gctcaatgtc
     1441 gcgcacaatg ggcttgtcca acgtatacgg aggcagcgaa caggctgaat cgccggaatg
     1501 aaccccggct tcttcgatat gctccatgat tcccgcaacc accaccgttt cgccgtccga
     1561 aatcgcatcg gcatcgactt cgatggcatc ggcaaggtac ctatcgatca ataccgggtg
     1621 attgggcgac gccttgaccg cagagtgcat gtactccagc aagccggctt cgtcatacac
     1681 gatctgcatt gaacggccac ccaacacgta ggacggacgg accatgaccg gatagctgat
     1741 ccgcctggcg atctgcaccg cttcctcgat ggatcgggca gacccacttt ccgcctgctt
     1801 taagcccaat ctattcaaga gatcccgaaa tcgttccctg tcttccgcca aatcgatggc
     1861 atcgggactg gtaccaagaa tcctcacgcc ggctttcgac aggggaagcg ccaatttcaa
     1921 aggcgtctgt ccgccgaatt gcaacacgac tccgatcggc tgttcccgat ggacgatatt
     1981 caggacgtct tcatgcgtca gcggctcgaa gtacagccga tctgacgtat cgtaatccgt
     2041 gctcacggtc tccgggttgc agtt
//
LOCUS       DASTWE010000225         1345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_70693, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000225 DASTWE010000000
VERSION     DASTWE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70693"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFT30_02445"
     CDS             <1..993
                     /locus_tag="VFT30_02445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5091510.1"
                     /translation="TKYSMEETVLDPSLRLRPVKWSDLEAVTQLIYDVCVADGDSVVA
                     VTSEELRHEWETPGFVLERDAFLIETSDGRIAGYEEFFNEHEHVRLRTDGYVHPDFKG
                     RGIGTSLLRMIEKRAREEILLAEPNMRVTLHSMTDHRDPDGRNLHQNEGYQPLRYHWR
                     MEIVLDGPPAEPKLPQGIELRAFVKGEHDVPVWQAQNEAWRDHWGSHDVTLEEWKRSR
                     FDDPEFDPTLWQIAWDGDEVAGFSLNRYRMGIGWIRTLGVRRPWRKRGLGEALLLQSF
                     GEFYKRGTKTIGLGVDAQNPTGATRLYQKVGMYIASEYVTYEKELRAGRDIDEE"
     gene            1085..>1345
                     /locus_tag="VFT30_02450"
     CDS             1085..>1345
                     /locus_tag="VFT30_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091511.1"
                     /translation="MSTQLQKELIVELPEGFTARGARMEDVEPALALYNRWSRSVIGR
                     DEITDIQAVRNEWLSPNFDPAEDIRLVFAPNGLMVGFIEVWTT"
BASE COUNT          319 a          339 c          399 g          288 t
ORIGIN      
        1 acaaaatact caatggagga aacggttctt gacccaagcc tccgtttgcg tcccgttaag
       61 tggagcgatc tcgaagcggt gacgcaattg atctacgatg tctgtgtggc ggatggcgat
      121 tctgtcgtcg ctgtaacttc agaagaactg agacatgaat gggaaacacc gggctttgtc
      181 ctggagagag acgcgtttct tattgaaacc agtgatgggc gcattgccgg ctacgaggaa
      241 tttttcaatg agcacgaaca tgtacggctt cgaaccgatg gttatgttca cccggacttc
      301 aaaggacgtg ggattggaac atctttatta cgcatgattg agaagcgcgc gcgcgaagag
      361 atcctcctgg cagagccgaa tatgcgggtc acactgcaca gcatgaccga tcaccgcgac
      421 ccagacggcc gcaacttgca ccagaacgaa ggctaccagc cattgcgtta tcactggcgt
      481 atggagatcg tgctggacgg tccgcctgca gaaccgaagt tgccgcaggg gatcgaactg
      541 cgtgcctttg tcaaaggcga gcatgacgtc cccgtctggc aggcgcagaa cgaagcctgg
      601 cgcgatcatt ggggcagcca tgatgtgacc ctagaggaat ggaagcgcag ccggttcgat
      661 gacccggaat tcgacccgac tctctggcag attgcctggg atggggatga ggttgccggc
      721 ttttccttga atcgttaccg catgggcatt ggctggatcc gcacgctggg agtccgccgt
      781 ccctggcgca agcgcgggct cggggaagca ttgctgcttc aatcgtttgg tgaattctac
      841 aagcgcggta caaagaccat tggtctgggt gtggatgcgc agaatcccac cggcgcgacc
      901 cgcctctatc aaaaagtagg catgtacatc gccagtgaat acgtcaccta cgagaaagaa
      961 ctgcgcgccg gtcgtgatat cgatgaagaa taagcaccgc agagacgcac gggctctgaa
     1021 aaattctaag actccgtgtc tttatgatcc ccgtcagggg actgtggtta gtaagggaat
     1081 aaaaatgagc acacaattgc agaaagaatt gatcgtcgaa ttgcccgagg gtttcacagc
     1141 ccgcggtgct cgaatggaag atgtggaacc agctctcgcg ctctacaacc gctggtcgcg
     1201 ctctgtgatc gggcgggatg agatcaccga tatacaagct gttcgcaatg aatggctgtc
     1261 accgaacttc gatcctgcgg aggatatccg tctggtcttc gcgccgaacg gattgatggt
     1321 tggcttcatc gaagtgtgga ccact
//
LOCUS       DASTWE010000226         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_620074, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000226 DASTWE010000000
VERSION     DASTWE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620074"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFT30_02455"
     CDS             <1..840
                     /locus_tag="VFT30_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU5091512.1"
                     /translation="LASAITTIGLDHQEYLGHTEEAIAFEKGGIIKPFVPVVIGRMGQ
                     EAEYVLCRIAKDRSAPLWKLGKDFAVDGNRTEGLTYRGATRVFENLTCGLAGRHQWDN
                     AACALALLEAAGQGGIETDVAAIRDGLRTVSWEGRLESINEYPTVVLDGAHNTAAAHA
                     LAGYLKDFSISHPASRIILVWGMMRDKDRRGVIAPLLPFVSEIVLTQATLARSATLQE
                     LRATLHEWHGPVLEAVLPMDALASARSRAMPHDLICIAGSLMLLGDIKAAVRGCGLSP
                     IRG"
     gene            842..>1510
                     /locus_tag="VFT30_02460"
     CDS             842..>1510
                     /locus_tag="VFT30_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEU5091513.1"
                     /translation="MTGPLEWIRWRRVLFVAVLSLVPFPGSAADSQVGAGTAPAGSAP
                     LDITADRIDYRQDQDVYEANGSVLIQQGAIKLMADHATIQTLPGILTAVGHVHLTDPQ
                     ADVTAERMELNINTEAGVVTHGRLYVPTTNSSISGRLMQRFSEYHYRVKEGSFTNCDT
                     QDGEVPTWRFRFKDLDMTLGDRFGFNGAWFCVLDVPTIPLPTFSYPMTKRQTGFLMPI
                     PSYDN"
BASE COUNT          321 a          401 c          444 g          344 t
ORIGIN      
        1 ctggctagtg ccatcacgac gatcggtttg gatcatcagg agtatttagg acacacggag
       61 gaagcgatcg cgtttgaaaa gggaggcatc atcaagccct ttgtgccggt cgtgatcgga
      121 cggatggggc aggaagccga atacgttcta tgccggattg cgaaggatcg ttcggcgccc
      181 ttgtggaaac ttggcaagga ctttgctgtc gatggaaatc gtaccgaggg acttacgtat
      241 cgtggagcga ctcgggtgtt tgagaatctg acctgcggcc tggccgggcg ccatcagtgg
      301 gacaatgccg cttgtgcgtt ggcactcctt gaagcggcag gtcaaggagg aatcgagacg
      361 gatgtggcag ccattcgtga tggcctgcgt acggtctcat gggaaggtcg tttggagtcg
      421 atcaacgaat atcccacggt ggtactcgac ggcgcccaca atactgccgc tgcccatgcg
      481 ctggcggggt atctcaagga cttttccatc agtcatccag catctcggat tatcctggta
      541 tggggcatga tgcgagataa agatcgacgg ggagttatcg cgccactgtt gccgtttgtg
      601 tcggaaatcg tgctgacgca ggcgactctc gcacggtcgg ctacacttca agaactccgt
      661 gcaactctgc acgaatggca tgggccggtg ctcgaggccg tccttcccat ggacgcattg
      721 gcatccgcca gaagccgagc catgcctcac gatctcatct gtattgccgg atcattgatg
      781 ctcctagggg atatcaaagc ggcagtgcgc ggctgtgggt tgtcaccgat tcgtggttag
      841 catgacgggt cctctcgagt ggatccgctg gcggcgtgtt ctcttcgtcg ccgtcctctc
      901 tctcgttcca tttcctggat cagcggcaga cagccaagta ggtgccggaa cggcgcccgc
      961 tggatctgct cccctcgata tcacggcaga ccgtatcgac tatcgccaag atcaagacgt
     1021 ctatgaagca aatggatcgg tcttgattca gcagggcgca attaagctca tggcggacca
     1081 cgccaccatt caaaccttgc cggggattct cactgccgtc ggccatgtcc atttaacgga
     1141 cccgcaggct gatgtgacgg ctgagcggat ggaactcaat atcaatactg aggccggcgt
     1201 cgtgacgcat ggccggctct acgttccgac gacaaattca tcaatatcag gtcgcctcat
     1261 gcagaggttt tctgaatacc actaccgagt caaggagggc agcttcacga attgtgatac
     1321 gcaagatgga gaagtgccga catggcgatt tcggtttaag gacctggata tgaccctcgg
     1381 ggacaggttt ggattcaatg gtgcctggtt ctgtgtattg gacgtgccga caattccact
     1441 acccaccttc tcctatccga tgaccaaacg acaaaccgga tttctgatgc cgatcccgag
     1501 ctatgacaat
//
LOCUS       DASTWE010000227         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_426194, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000227 DASTWE010000000
VERSION     DASTWE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426194"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /gene="ureC"
                     /locus_tag="VFT30_02465"
     CDS             complement(<1..396)
                     /gene="ureC"
                     /locus_tag="VFT30_02465"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012663.4"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEU5091514.1"
                     /translation="MKISRKQYASLYGPTVGDRVRLGDTDLIIEVEEDRIVAGEEAVF
                     GGGKTIRDGMGQSARATSTSGQLDTVITNALILDYTGVIKADIGIKDGRIVGIGHAGN
                     SDLMPNVTPGMEIGPGTEAISGEGRIITAG"
     gene            complement(393..1064)
                     /locus_tag="VFT30_02470"
     CDS             complement(393..1064)
                     /locus_tag="VFT30_02470"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEU5091515.1"
                     /translation="MKAKESRDSGYDKEKRSTSEKTGPSRRQFLAHSGRVAAGIGAVS
                     LLGNAGQHGIAYAANESLITKVGGTGGAKMKKQDAPQRSKEALAAAVKAELSAPIVPG
                     EIMTVAGDIEVFKGRETKEMTIKNVGDRPIQVGSHCHFFESNRALKFEREQAFGFRLA
                     IPAGTAVRFEPGEEKKVPLVAFAGNRLAQGVNGLTEGLLDDPKVKAKAISLAADRGFG
                     KGGAR"
     gene            complement(1161..1295)
                     /locus_tag="VFT30_02475"
     CDS             complement(1161..1295)
                     /locus_tag="VFT30_02475"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEU5091516.1"
                     /translation="MTYGTQLLSRKDVMPGVPEMIREFQIEGTFPDGTKLVTVHNPIP
                     "
BASE COUNT          261 a          391 c          313 g          333 t
ORIGIN      
        1 cccggctgtg atgatacgac cttctccgga gatcgcttct gttcccggac caatctccat
       61 gcccggcgtg acattcggca tcaaatcgga atttccggca tgaccgatac cgacgattcg
      121 gccgtccttg atgccgatgt cggccttgat gacaccagtg tagtcaagga tgagtgcatt
      181 ggtaatgaca gtgtcgagct ggccgcttgt gctcgtggca cgagcggact gccccatacc
      241 gtcacgaatg gtcttgccgc cgccgaagac ggcttcctct ccggcaacga tccgatcttc
      301 ttcgacttcg atgataagat ccgtgtcgcc aagtcggaca cgatcgccga ccgtgggacc
      361 gtacagagat gcatactgct tgcgtgaaat tttcaccggg cgcctccctt tccaaatccg
      421 cgatcagcgg caagtgaaat cgccttagct ttgactttcg gatcatccaa caatccctcg
      481 gtcaatccgt tcacgccttg ggcaagccgg ttacctgcaa aagccacgag cggcactttt
      541 ttttcttctc ccggttcaaa tcggacagcc gtaccggcag gaatagcaag acggaacccg
      601 aacgcttgct cacgctcaaa cttcagcgca cgattggatt caaagaagtg acagtgagaa
      661 ccaacctgaa tcggtcgatc acccacattc ttaatagtca tttcttttgt ctcccgtccc
      721 ttgaagacct cgatatcacc ggccacagtc ataatctcgc cgggcacgat cggggcagac
      781 agttcagctt tcactgcagc cgccagggct tcttttgatc tctgtggcgc gtcttgtttt
      841 ttcattttcg ctcctcctgt cccaccgacc tttgtgatca gcgattcatt tgcagcatac
      901 gcaatcccgt gttgcccagc gtttcccaat agtgataccg ccccgattcc tgccgcaacg
      961 cgcccgctgt gcgcgagaaa ctgccgccgc gacgggccag ttttctcgga agtacttcgt
     1021 ttttccttgt cgtatccgga gtctcgagac tctttcgctt tcatgttact tcactccttt
     1081 ttcgcgcgcc gtgtcggttt ccaataattc ttcatctcct caggcatttc cgtcatgaga
     1141 gttggctacg gtatgtacat ctaaggaatg ggattgtgaa ccgtcacgag tttcgtccca
     1201 tcgggaaagg tcccttctat ctggaactca cggatcatct ccgggacacc gggcatcaca
     1261 tctttgcgcg acagaagctg ggtaccgtag gtcatgag
//
LOCUS       DASTWE010000228         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1105068, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000228 DASTWE010000000
VERSION     DASTWE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105068"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFT30_02480"
     CDS             <1..418
                     /locus_tag="VFT30_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091517.1"
                     /translation="TESRERPSQREPSGQTRRVSSAKLPMSLPSNQEVTLSAFTYKVL
                     EANLDRLAPSKLRLQFRIRLTNNDGYPTNFWNRTFRLLEDGVPRSPISDLNESVEGHS
                     AKEGLVEFAVPESVQHVLLQVRQGDDVAELQFILPQ"
     gene            complement(516..1199)
                     /locus_tag="VFT30_02485"
     CDS             complement(516..1199)
                     /locus_tag="VFT30_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5091518.1"
                     /translation="MIHNLLSDSTVGATFRRVRIVQCLVVGLMVLATNGPTVALVEQS
                     PMDLVRSTITKVFRILDQDELKQPGHAVERRQQIEQVLRDRVSYEEMAKRALGSAWSE
                     LTVAERREFVGLFVQLLRDQFACKIDIYADEHVQYLSEQRRANMAEVRTILTGAKTVT
                     RLDFRLTDRTGEWMVYDVMIDGASIVSNYRAQFASIMRNASYTDLVERMKEKALVVKA
                     FEQDTRHDE"
BASE COUNT          383 a          487 c          392 g          387 t
ORIGIN      
        1 gacggaatca cgtgagcgtc cgagtcagcg ggaaccatcc ggtcagactc gtcgggtttc
       61 atccgccaaa ctcccgatgt ccctcccctc gaatcaagag gtcacacttt ctgccttcac
      121 ctacaaggtt cttgaggcaa acctcgatcg ccttgcccct tcaaaacttc ggttacagtt
      181 ccgcatccga ctcacgaaca acgatgggta tcccactaac ttctggaacc gaacgttccg
      241 attgctggag gacggggttc cacggtctcc gattagtgat ctgaacgaat cggtcgaagg
      301 ccatagcgcc aaggaaggtc tggtggaatt cgctgttcct gaatcagtgc agcatgtgct
      361 gcttcaggtg cgtcagggag atgacgtggc cgaactgcaa tttattcttc cacaatagct
      421 tcagcttcaa cccggtcggc ctagggtctt catctgttat gccctcgcct acacacttac
      481 caacagagta atttcacctt gaacgagcaa taaggtcatt catcgtgtct cgtgtcctgt
      541 tcgaaggctt tcacgaccag cgctttctct ttcatgcgtt ccaccaagtc ggtataggaa
      601 gcgttgcgca tgatgctcgc aaactgcgcg cgatagttac tgacgatact cgcgccgtcg
      661 atcatcacat cgtaaaccat ccactcaccc gtgcgatcgg tcaaccggaa gtccaaccgt
      721 gtaacggtct ttgcgccagt taagattgta cgaacctcgg ccatattcgc ccttcgctgc
      781 tccgagaggt actgcacatg ctcgtcagca tagatgtcga tcttgcacgc gaattgatcc
      841 cgcagcaact gcacaaaaag accgacgaat tctcgtcgtt ctgcgaccgt cagttcactc
      901 cacgccgacc ccaatgcccg ttttgccatc tcttcgtagc tgacacgatc ccgcagcacc
      961 tgttcgattt gctgtcgccg ttctacggca tgcccaggct gtttcaactc atcctgatcc
     1021 aggatgcgaa acaccttcgt gatcgtactc ctcacgagat ccatcggcga ctgctcaacc
     1081 agcgccaccg tcggcccgtt cgtggccagc accataagtc ccacgactag acattgaaca
     1141 atgcggactc tacggaaagt tgcaccgaca gttgagtcgg aaagcaggtt gtgtatcacg
     1201 tacggccctc cggtacccca ttaggaacat caggaacatc tgctggactg tcaagctgag
     1261 caagagccgt gctgaaccgg tcgcccgaag gtcctggaag gatgaccggg agaaaaagtc
     1321 cgtaactcac tgaccttgtt gagacagtag attgattctc ttctttgctc taatgagcac
     1381 accccagcga gccggctcct gaaggatctg aatggcgcat tggtgtggtg ttaaggacgc
     1441 atatgtagat aacggaaaga cgcacgagcc cgtattaagt tgtcctccgg ctaaatcatg
     1501 ggatccattc atctgatggc catacgcaga ggctgttcca aaattatctc tgttatgcct
     1561 tcgcctacaa tactcactga ccgcatcatt ctaggctaac agttctgaac ccgctgcgaa
     1621 cgggttcacc ggcagcagat caccaaact
//
LOCUS       DASTWE010000229         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_814101, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000229 DASTWE010000000
VERSION     DASTWE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814101"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VFT30_02490"
     CDS             <1..141
                     /locus_tag="VFT30_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU5091519.1"
                     /translation="DEAKTLEEIGRTMGLTRERVRQIEMAALVRLRHTIERKTMTQAD
                     LL"
     gene            146..670
                     /locus_tag="VFT30_02495"
     CDS             146..670
                     /locus_tag="VFT30_02495"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5091520.1"
                     /translation="MTAVNYQAFIREVPDFPKPGILFYDITTLLKNPAAVRSLADQLT
                     TRYQDRRITKVVGIESRGFIFGGILAARLGAGFVPVRKPGKLPADCYEVKYSLEYGHN
                     SLAVHRDAIEIGEHVLIVDDLLATGGTAEATVHLVRQLGGTIVGLDFLVELKSLKGRD
                     KLADYDVHSTITYP"
     gene            782..1345
                     /locus_tag="VFT30_02500"
     CDS             782..1345
                     /locus_tag="VFT30_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU5091521.1"
                     /translation="MATKTQAKKKGETKTKSVASTAKKPQPVTAGAAAAFKSTPEVEI
                     EVPVRPKETAKEREARERRQEVLQKMLIGKRQEIIKEIEESLGQSLTEDQQRRLESAR
                     DVGDQALMDLERELGISLMEMRNRRRQSIDEAITRLHEGTYGICAECGVEISEKRLQA
                     VPFAKLCVECQSRAELLEKIEREEERD"
     gene            1394..2092
                     /gene="queC"
                     /locus_tag="VFT30_02505"
     CDS             1394..2092
                     /gene="queC"
                     /locus_tag="VFT30_02505"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU5091522.1"
                     /translation="MTGQISERAVVLASGGLDSTVTAAVARRDGYALHFLTIAYGQRH
                     AVEVERSRQVATALGAHQHVVIDVDLRAIGGSALTDDISVPKRRTESEWNRDVPVTYV
                     PGRNLIFLALAAAHAEVLGASVVYFGANVIDYSGYPDCRPEFIKAVEAALQAGTKAGM
                     QGKSIELRAPLLRMTKAEIIRLGLKLNVPFHLTHSCYDPVGSVACGQCDSCLVRKRGF
                     AEAGVVDPIQYAVC"
     gene            2121..>2455
                     /locus_tag="VFT30_02510"
     CDS             2121..>2455
                     /locus_tag="VFT30_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5091523.1"
                     /translation="MKTSRMLWVSLVVPILGAAPACSQNGMEPQGTGTVTSAPAELRE
                     GEQKFNTNCSTCHGIGGVGTTQGPPFLHKVYEPNHHGDAAFQRAAANGVKAHHWQFGD
                     MPKIDAVKP"
BASE COUNT          597 a          620 c          718 g          520 t
ORIGIN      
        1 gacgaagcga agacgctcga ggaaatcggc cgtaccatgg ggctgactcg tgagcgcgtc
       61 cgacagatcg agatggccgc gctagttcgc cttcgccaca ccatcgagcg aaagaccatg
      121 acacaggcag acttgctcta atctcgtgac cgccgtcaac tatcaagcat tcattcgaga
      181 agtgccggac tttccgaagc ccggcattct attttatgac atcaccacgc tcctgaagaa
      241 tcctgccgcg gttcggagtc ttgccgacca attgacgact cggtatcagg atcggaggat
      301 caccaaggta gtagggattg aatcccgggg gtttatcttc ggcgggattc tcgcggcgcg
      361 tctcggggcc ggatttgttc cggttcgtaa gcctggaaaa cttccagctg attgttatga
      421 agtgaaatat agtctcgagt acggacataa cagtcttgcc gtgcatcgtg atgcgatcga
      481 aatcggagag catgtgctga ttgtcgatga cctcttggcg accggaggaa cggcagaagc
      541 gacggtccat ctcgttcgtc aacttggtgg cacaattgtc ggactcgatt ttctggttga
      601 actgaagagt ttgaaggggc gcgacaaact ggctgactac gatgtccatt cgaccatcac
      661 ctatccctag gtttgaacgc atccttcatc accctcttgt tcaagaggcc caggccgtga
      721 tatagaagcc ggaatttttt gaccctccaa ccacttattc aacttctgga gcggactctc
      781 tatggcaacg aaaacacagg cgaagaaaaa gggcgagaca aagacgaaat ccgtggcttc
      841 tacggccaag aaaccacagc cggtgactgc gggagcggct gcggcgttca aatcgacgcc
      901 cgaggtcgaa atagaggtgc ctgtgcggcc taaggagacg gcaaaagagc gggaagcacg
      961 agaacgacgg caggaagtgc ttcaaaagat gctcatcggt aaacgccaag agattattaa
     1021 ggagatcgaa gagagtctgg ggcagtcttt gacagaagat cagcaacggc gtctggagtc
     1081 agcgcgtgat gtcggcgacc aagccttaat ggatcttgag cgcgaactcg gtatttcctt
     1141 gatggaaatg cgtaaccgcc ggcgacagtc gatcgacgaa gccattactc ggctacacga
     1201 aggaacttac gggatttgtg ccgaatgtgg tgtcgaaatc agcgagaagc gtctccaggc
     1261 agtccccttc gccaagctgt gtgtggagtg ccaatcgcgc gcagaattgc tggagaaaat
     1321 cgaacgcgaa gaagagcgcg attaactctc aagaattcat ccccacggca cgcctgcatc
     1381 atctttttcc atcatgactg ggcagatttc agaacgagcg gtcgttcttg ccagtggcgg
     1441 gttggactcc actgttacgg cggctgtcgc ccgccgtgac gggtacgcgc tgcatttcct
     1501 caccattgca tacggacaac gtcacgctgt ggaggtcgag cggtcgagac aggtggcgac
     1561 ggctctaggg gctcaccagc atgttgtcat agacgtcgat ttgagggcga tcggcggatc
     1621 ggccctgacc gacgatatat cggtgccgaa gcgtcggact gaatctgaat ggaaccggga
     1681 tgtgcccgta acctatgtac cgggccgaaa cttgattttt ctggctctag ctgcggctca
     1741 cgcggaagtg ctgggggcct cggtcgtcta tttcggcgcg aacgtgatcg attattcggg
     1801 ctatcccgac tgccgaccgg aattcattaa ggccgtggaa gcggcactcc aagccggaac
     1861 aaaggcagga atgcagggca aaagcattga gcttcgagcg ccgctgctcc ggatgactaa
     1921 ggcagagatt ataaggcttg gcttgaaact aaacgttccc tttcatctca cgcatagttg
     1981 ttatgacccg gttgggtcag ttgcttgtgg gcagtgcgac agctgtctgg ttcgtaaacg
     2041 gggatttgcg gaagcgggag ttgtggatcc gattcagtat gcggtatgtt gagccaacga
     2101 tcaaactcga ataacgatct atgaagacat cacgaatgct gtgggtgagt cttgttgtgc
     2161 caatccttgg ggccgcacca gcatgcagcc agaacgggat ggaaccacag ggaacaggaa
     2221 ccgtgacctc tgctccggcg gaattacggg aaggagagca gaaatttaac accaactgtt
     2281 ccacctgcca cgggatcggc ggagtcggga ccacccaagg tccaccgttt cttcacaagg
     2341 tctatgagcc gaatcatcac ggtgatgcgg catttcagcg agcagcagcc aatggagtaa
     2401 aggctcatca ttggcaattc ggcgacatgc caaagatcga tgcagttaaa ccaga
//
LOCUS       DASTWE010000230         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_846518, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000230 DASTWE010000000
VERSION     DASTWE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846518"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..1175)
                     /locus_tag="VFT30_02515"
     CDS             complement(417..1175)
                     /locus_tag="VFT30_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5091524.1"
                     /translation="MLKLVGVTKTLGGQPVLRGIDLIVPEGKLTTVIGRSGEGKSVLL
                     KHMIGLLQPDSGQVWVDGVEISRLHGHALNEVRKRFAMLFQGAALFDSLTVFENVAFP
                     LRERLRMKGQDVTSRVEEKLDQVGLAGMGHKFPAELSGGMRKRAGLARALVMQPEIIL
                     FDEPTTGLDPLMAKSIHDLITSMQRKFGFTAVMVSHQIPEIFGISDYVAMLKSGRIVA
                     MAEPAELQRTTDLEIREFISVGGTVPLTKAGSAD"
     gene            complement(1178..1945)
                     /locus_tag="VFT30_02520"
     CDS             complement(1178..1945)
                     /locus_tag="VFT30_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEU5091525.1"
                     /translation="MTLLELLGRWALTYVAEMGRMLIFVVASFAWLARPPLRGMQIVK
                     QLHFIGYKSTFVVVLTAAFTGMVLALQGYYTLRKFGSEGLLGSAVALSMIRELGPVLA
                     ALMVTARAGSAITAEIGIMRITEQIDALDTMAVNPLQYLIAPKLVAGLIGVPLLVAIF
                     DVVGIYGGHLVGVDLLGVNAGSYWNSIEAAVEWKDVYGGILKSISFGLIVSWVCCYKG
                     FYTKMSAEGLGTATTEAVVLSSVLILIWDYFLTSLLL"
     gene            complement(1948..>2542)
                     /locus_tag="VFT30_02525"
     CDS             complement(1948..>2542)
                     /locus_tag="VFT30_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091526.1"
                     /translation="DNQSFSLMVSTGFACALIAVDIGTLLVGREVMHRGAHSDESTEV
                     HEVPGDERDLVMALAETLVPPEHIGRFVSTDRIIGSAPEKRRFALSTQAIGLDMESSA
                     LAAQAQRARVPFVIIRSVSDLLDEDLPLDFNLFLRPTGWLKGIGTVLAAPSRLLGLGR
                     LRRQSLVAAEALTAFFRSYAAAMATERQNRKKDLSPT"
BASE COUNT          599 a          807 c          613 g          523 t
ORIGIN      
        1 gcatccacct cgtaaacatc tccaccgata agttccaact ttccgagttt aatcgacaga
       61 tagcccaaac agacaatccc gaccagcacg aacacgccga cgatgagttc taacttgctt
      121 ttgtccatca tgacactcct ctagcaggct gttgagaaaa tccgccagcg gcgttctcgc
      181 agatatagca gctcaccatc tcggcgtcgt ccacagacgt gccgtacctt attcggcatg
      241 gcgtggacct cgcgcccatc cacaaacgtg acgctcatta ttcttcgcgt cacggacctt
      301 gctgcggcct tgctggacga gcctttttga ccaacctccg ggctgttgct gttatagatg
      361 gtccggcccc cgatcttctc tttttccaca cttgcaaaat agcctgtcaa catcccctag
      421 tccgcggaac ctgccttggt cagcggcacc gtcccaccga ctgaaatgaa ctccctgatt
      481 tcaagatcgg tcgtccgttg caattcagcc ggctccgcca tcgcgactat tctcccgctt
      541 tttaacattg cgacataatc cgaaatcccg aatatctccg gaatttgatg gctcaccatc
      601 acggcggtaa acccgaactt ccgctgcatg ctcgtaataa gatcatgaat cgatttagcc
      661 atcagtggat cgagacccgt cgtgggctca tcgaagagaa taatttccgg ttgcatcacc
      721 aacgcacgtg ccaggccggc gcgtttccgc atgcccccgc tcagttccgc ggggaacttg
      781 tgtcccatcc ctgccaggcc tacctgatcc aacttctctt cgacgcggct agtcacatcc
      841 tggcctttca ttctgagtct ttctctgagc ggaaacgcga cgttttcgaa gacagtcagt
      901 gaatcgaaca gcgccgcccc ctgaaacaac atcgcgaacc gcttgcgcac ttcgttgagt
      961 gcatggccgt ggagccgaga gatctccact ccatcgaccc acacttgtcc ggaatcaggt
     1021 tggaggagtc cgatcatatg cttgagcagc acgcttttgc cttcaccgct tcgaccgatg
     1081 accgtcgtga gctttccttc aggaacgatg agatcaatgc cccgcaagac cggctgacct
     1141 cccaacgtct tcgtcacacc cacgagtttc agcatgatta caacaacaat gatgtcagaa
     1201 aatagtccca aatgagaatc aagaccgatg acaacacgac cgcctctgtc gtggcggtgc
     1261 cgagcccttc ggcactcatc ttggtataaa agcccttata gcaacagacc cagctcacga
     1321 tcagtccaaa actaatggac ttaagaatgc cgccatagac gtccttccat tcgaccgccg
     1381 cctcgatgga attccagtac gaaccagcgt tgacgccgag caggtccact ccaacaagat
     1441 gaccaccgta gattcctacc acgtcgaaga tagcgaccaa caatggcaca ccaattaagc
     1501 cggcgacgag tttcggcgca atcaggtact ggaggggatt gacagccatg gtatcgagcg
     1561 cgtcgatctg ttccgtgatc cgcatgatgc cgatctccgc cgttatagcg gaaccggcgc
     1621 gcgccgtcac catgagcgca gccaagactg gacccaactc gcgaatcatg ctgagcgcca
     1681 ccgccgaacc taacaaccct tcagacccga actttcgtaa ggtatagtag ccttgcagcg
     1741 ccagcaccat cccggtgaag gccgccgtca gcacgacaac aaacgttgac ttgtagccga
     1801 tgaagtggag ttgcttgacg atttgcatgc cccgcaacgg cggacgtgcc agccaggcga
     1861 aggacgccac cacgaagatc agcatccggc ccatttcagc cacataggta agagcccatc
     1921 ggcccaagag ttccagtagc gtcatggtca agtcggcgag aggtccttct tccggttctg
     1981 acgttcggtc gccatcgccg ccgcatagct ccggaaaaag gccgttaaag cctccgctgc
     2041 gaccaaactt tgtcggcgaa gccggcccag acccaagaga cgtgaaggag cggccaagac
     2101 agtaccgata cctttgagcc acccagtggg cctgagaaat agattgaagt caagcggaag
     2161 atcttcgtcc agaagatccg aaacagatcg aatgatcaca aagggaaccc tcgcccgttg
     2221 cgcttgggca gccaaggcgg aactttccat atcgaggccg atggcctgag tactcagcgc
     2281 aaatcttcgc ttttccgggg cgctgccgat tattcgatcg gtcgagacaa atcgcccgat
     2341 gtgctcgggc ggcaccagag tctcggccaa cgccatgacg agatcccgct catcacccgg
     2401 cacttcgtga acctctgtcg actcatcgct gtgcgctcct cgatgcatca cttcacggcc
     2461 taccaagagc gtcccgatgt ccaccgcaat gagtgcacag gcaaaccccg tcgacaccat
     2521 gaggctgaac gactggttgt cc
//
LOCUS       DASTWE010000231         1889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_523305, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000231 DASTWE010000000
VERSION     DASTWE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523305"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFT30_02530"
     CDS             <1..798
                     /locus_tag="VFT30_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU5091527.1"
                     /translation="VAMGHVQTVTDDYVQLRAPSTDILALPPFKREDYVTTHEVEISH
                     LEDNLHVTPGEVLVPLPDLEKNVKRRTFFMNFTHVIGFLIGLPIAYPIVRFLMKPMYA
                     EFNNEWLKVGNVSKIKQDDVGVQFKYKKKVKEVYMPEYEVEKNVWVLRATSELLEKVY
                     KDKDMEFRDAKGKTIWTNKKTIPYVAFSGKCPHLGCAFKWRQHKTLGQVFLCPCHLSI
                     YDAAGKVLDGPAPRALDALPVRVSAGGEVEIIDMEFKAGTKSQTRIV"
     gene            810..>1889
                     /locus_tag="VFT30_02535"
     CDS             810..>1889
                     /locus_tag="VFT30_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEU5091528.1"
                     /translation="MDSKQSSSATIPDHQPSAIEKLVAFLDERVGLKEMQAKMLNEPI
                     PGGSRWAYVFGSILLFIFAMQALTGILLMFYYVPTADHAYASTQYIIHDVDYGWFLLG
                     YHFWGSSAMVVCVVAHMSQVFLWGAYKKPRELLWIVGLALFGIVITFGFTGYLLPWDQ
                     RAFWATTVGVEILDKTPVVGDFIARFLKGGPTPGQMTLSRFFVLHVMVLPAALMALAG
                     LHLFLFRVAAPAGPFTGTVEEIKAKTDYFFPRQIWKDMVGMAFVFVSICALALWEPVV
                     LLDEAAPDPGDYHPEPEWYFLFYFQLLRLKLFAGEFGQFLGAVALPVAFMVLLVALPF
                     IDKDPERNIFKRPIALISWIAIMVVI"
BASE COUNT          398 a          492 c          527 g          472 t
ORIGIN      
        1 gtcgccatgg gccatgtcca gactgtgacg gacgactacg tgcagttgcg cgcgccgtca
       61 acggacatcc tcgcgttgcc tcccttcaag cgggaagatt atgtcaccac gcatgaagtg
      121 gaaatctccc accttgagga caacctccat gtgacgccag gtgaagtgtt ggtgccgctg
      181 cccgatctgg aaaagaacgt caaacgccgc acgtttttca tgaatttcac gcatgtcatt
      241 gggttcttga tcggcctccc catcgcgtat cccattgtcc gctttctgat gaagccgatg
      301 tatgcggagt tcaacaatga atggctgaaa gtgggaaatg tcagcaagat caagcaagac
      361 gacgtcggcg tgcagtttaa gtataagaaa aaggtcaaag aagtctatat gccggagtat
      421 gaagtcgaga agaacgtctg ggttctccga gcgacctcgg agcttctcga gaaagtctac
      481 aaagacaagg acatggagtt tcgcgatgcc aagggaaaaa cgatctggac caataagaaa
      541 accattccct acgtggcgtt ctccggtaag tgccctcatt taggctgcgc attcaagtgg
      601 cgacagcata agacgctggg acaagtgttt ctctgtcctt gccacttgag catctacgat
      661 gcagcaggga aagtactcga cggtcccgcc ccgcgcgccc ttgacgcgtt gcctgtgagg
      721 gtatcggcgg gaggtgaggt cgaaatcatc gatatggagt tcaaggccgg tacgaaatcg
      781 caaacccgga tcgtttgaaa gctggcgaca tggatagcaa gcaatcttct tctgcaacga
      841 ttcccgacca tcagcccagc gcgatcgaaa agctcgtcgc tttccttgat gaacgtgtcg
      901 gcctcaagga aatgcaggcc aagatgctga acgaaccgat ccccggcggc tcccgttggg
      961 cctacgtgtt cggatccatc ttgttgttca tcttcgccat gcaggcactg accggcatcc
     1021 tgctcatgtt ctattacgtg ccgacagccg accatgccta cgccagtacg caatatatta
     1081 ttcacgatgt cgattatgga tggttcctcc tgggctatca tttctggggg tccagcgcca
     1141 tggtcgtctg tgtcgtggct cacatgtcgc aggtgtttct ctggggcgcc tataaaaagc
     1201 cgagagagtt actctggatc gtcggcctgg ctttgtttgg gatcgtgatc accttcggct
     1261 ttacaggcta cctcttgccc tgggaccaac gggcgttctg ggcgacgaca gtgggtgtcg
     1321 aaattttgga taagaccccc gttgtcggtg attttatcgc ccgatttcta aaaggcgggc
     1381 cgactccagg gcagatgacc ttgagccgat ttttcgtcct tcatgtgatg gttctcccag
     1441 cagcactcat ggctctggcc ggattgcatt tgttcctgtt tcgcgtggct gcacctgcgg
     1501 gccccttcac cggtacggtg gaggagatca aagcgaagac cgattatttc tttccccgtc
     1561 aaatttggaa agacatggtc gggatggcgt tcgttttcgt gagtatctgt gcattggccc
     1621 tctgggagcc ggtcgtctta ttggatgagg cggcgccgga tccaggggac tatcatccag
     1681 aacctgaatg gtattttctg ttctacttcc agctgctcag gctgaaactg ttcgccggtg
     1741 agttcggtca atttttgggt gccgtggcgt tgccggtcgc cttcatggtg ttgcttgtcg
     1801 cgctcccgtt tatcgacaag gaccccgagc ggaatatttt caagcgaccg atcgccctca
     1861 tcagttggat tgcgatcatg gtggtgatc
//
LOCUS       DASTWE010000232         1700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_38772, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000232 DASTWE010000000
VERSION     DASTWE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38772"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..324)
                     /locus_tag="VFT30_02540"
     CDS             complement(148..324)
                     /locus_tag="VFT30_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091529.1"
                     /translation="MKNVEMSIEGSVLTIRVDLSKEFGPSSTGKTVIIASTEGNVTIP
                     NRQEKVGLNVYRKK"
     gene            complement(499..960)
                     /locus_tag="VFT30_02545"
     CDS             complement(499..960)
                     /locus_tag="VFT30_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091530.1"
                     /translation="MKLRVFIVICGFLWASPAHADSPIAAQVCSLLTSPTLSALQSTT
                     ATGDGCAWQWPNGQVLSVYTHIDKQTETVAAMQEMLEAQLQNPVFKRTPFPVCTGGDM
                     IIGDFRNGKGPGGTGYTQCPGFVLTFRFQGIEAQKQLLSVAKKLAGTTFAR"
     gene            complement(1138..>1700)
                     /locus_tag="VFT30_02550"
     CDS             complement(1138..>1700)
                     /locus_tag="VFT30_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091531.1"
                     /translation="LACGAQPISAFPSVDVAPDRQTILVIPSNRATPSRLSIQSLPTE
                     STLAPGLSYHPQRFELVRVSPDGRYAAFSASGHHNLIGLLHLATMTVREIDLLTEGDV
                     VAFHWTADGRTLAYDYLPASGYRRVKGYDIESGEGLVIPRTERNSATHVTFESWGPRL
                     HEVILGVTDGRTNERWTETVTLIPRQ"
BASE COUNT          377 a          443 c          475 g          405 t
ORIGIN      
        1 acatggctat ccacagctac gagatttttc tggggatctc agactaattg ataggcctag
       61 agcgctgttt ctgacctgtc aagagccgat cggaaaagaa agcagcctcg cggtaaaaaa
      121 gatagggcca aatggctgcg agtggtgcta cttttttcgg tacacattga gcccgacctt
      181 ctcttgccga tttggaatgg tcacatttcc ttccgtcgaa gcaatgatga ccgtcttgcc
      241 tgtagaagag gggccgaact cttttgagag atccactctg atcgtcaaca cagacccctc
      301 gatgctcatc tcaacgtttt tcatgatcgc gccctttcat tagcacaaat ggtggttcct
      361 ttggtcttca acgaatgatc catgatccta tgggatctga tcacgaggtt gagctatggc
      421 cacgccaaag ccgagccgtg ggcgagttcg tcatctggct cgatttgaga atggcatctt
      481 cctcatggga tcaggaagct agcgtgcgaa ggttgtgccg gcgagtttct ttgcgacgct
      541 cagtaactgc ttctgggcct cgatcccttg aaagcggaag gtcagcacaa acccaggaca
      601 ctgagtatat ccggtgccgc cagggccttt accgtttcga aagtcaccga tgatcatgtc
      661 ccctcccgtg cagacaggga aaggtgttcg cttgaagacc gggttctgca attgggcctc
      721 gagcatctct tgcatcgccg ccactgtttc ggtctgctta tcgatgtgtg tatacaccga
      781 cagcacttga ccgttgggcc attgccatgc gcagccatcg cctgtcgctg tcgtagattg
      841 gagggctgat aaggtgggag atgtgagcag actacagact tgagctgcta tgggactgtc
      901 tgcgtgggcg ggtgatgccc agaggaatcc acagatgacg atgaaaaccc tcagtttcaa
      961 gatacgttcc ttggaagaaa aacacccgct actgatgagt cgcgccgtac cgccggtcat
     1021 ttacgttgcc gctgcgaaac gaacaagtaa attgtagtgt cggtgcctcg gctattcaat
     1081 agtctattgt tctaagtcca tccggatggc accggcagcg agggtgatac cggtcgtcta
     1141 ttggcgcgga atcaacgtga cggtttcggt ccaacgctcg tttgtgcgcc cgtcggttac
     1201 gcctaggatg acctcgtgca gccgcggtcc ccagctctca aacgtgacat gggtagccga
     1261 atttctctcc gtacgaggta tgactaaacc ttccccggat tcaatatcgt agcctttcac
     1321 ccgacgatag ccactggccg gcaagtagtc atacgcgagc gttcggccgt cggctgtcca
     1381 atggaaggcc actacgtccc cttccgtcag gaggtctatc tctcgcactg tcatggtcgc
     1441 aaggtgcaga aggcctatca gattgtgatg tcccgaagct gaaaacgccg cgtatcgtcc
     1501 gtccggtgaa acgcgaacca gctcgaatcg ttgcgggtgg taagacagac caggcgcaag
     1561 tgttgattcg gtgggcagac tttgaatcga gagtcgagag ggtgtcgctc gatttgaggg
     1621 aatcaccaga atagtttgtc gatcgggcgc cacgtccaca gatggaaaag cggatatggg
     1681 ctgagcaccg caggccagta
//
LOCUS       DASTWE010000233         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1396034, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000233 DASTWE010000000
VERSION     DASTWE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396034"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFT30_02555"
     CDS             <1..195
                     /locus_tag="VFT30_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091532.1"
                     /translation="EPNTVEHGATTCSEETILTFTAYGPIIFYNEDGTPGFVLDWEFV
                     KNQAEGAKVDPKFSVAKSAA"
     gene            488..>1252
                     /locus_tag="VFT30_02560"
     CDS             488..>1252
                     /locus_tag="VFT30_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5091533.1"
                     /translation="MQVIDGDSHFMEPLDLFERYIDPQFREQAMRVVQDPTTGKPALV
                     VEGRQLRIVNVEELLAAVVGYGQKEEGRNLSNFDPTLMYNAGWQDMDKRVQFLDQEGI
                     DRQILYPTMGLLWEGGVESPLVADALCRAYNTWAFEITASRKNRLFPAAHISLRDPQL
                     AVKELRRVAELGCKTLFVAAMPINGRSFGHPDFDPVWATIQDLDLSVGIHLVGHRHYT
                     GSQWYQDRDPGFMFVTMNVIQDPRMALTTMVYDGVFE"
BASE COUNT          289 a          310 c          356 g          297 t
ORIGIN      
        1 gaacctaaca cggtcgagca cggggcaacg acctgttcgg aagaaacgat ccttaccttc
       61 acggcctacg gtccgatcat tttctacaac gaagacggaa ccccggggtt cgtactcgat
      121 tgggaatttg tcaagaacca agccgaaggt gcgaaggtcg acccgaaatt tagtgtcgct
      181 aagagcgcgg cgtaataggt tgtcgccgga aacgcattgc ttattccggc ctgtattact
      241 acattccgct ttcgagtagg ccgggataag gtcggagact gctcccggct ttctgtttgc
      301 ctctataacc ccctgcaaga cttgctatgc acgaatgtag atagagatat ctctacgaaa
      361 cctctgtagg agggatgttt gatgtctctg ccgtatagca cggacggcct cccgtgcaac
      421 tgacggcatc tagagtgcgt actgtgtgtc caaggacggc taggccttaa ttaaggagga
      481 actttctatg caagtcatcg atggtgatag ccactttatg gaaccgttgg acctctttga
      541 acggtatatt gatccacaat ttcgtgaaca ggcaatgcgg gtggtacaag accctacgac
      601 cggcaaaccg gcgttggtag tagaagggcg gcagttgcgg attgtgaacg tcgaagagct
      661 ccttgctgcc gtcgtcggct acggccaaaa ggaagaagga cgtaacctga gcaatttcga
      721 tccgaccttg atgtataacg ccggatggca agacatggat aagcgggtgc agtttctcga
      781 tcaggaaggg atcgatcgac aaattctcta cccgacgatg ggtctcttat gggaaggcgg
      841 ggtagagagc ccgctcgtgg ccgatgcgct ttgccgcgcc tacaacacct gggcattcga
      901 gattaccgcc tcacggaaga acagattgtt tcctgccgcc cacatttcac ttcgtgatcc
      961 acaactggcg gtgaaagaac ttcgccgtgt tgccgagctt ggctgtaaga cgctttttgt
     1021 ggcggcaatg ccgatcaacg gccgcagttt tggtcacccc gattttgatc cggtgtgggc
     1081 aacgatacag gacctcgatc tgagcgttgg cattcatctg gtcggtcatc gccattacac
     1141 cggcagccaa tggtaccagg atcgtgatcc gggattcatg tttgtgacga tgaatgtgat
     1201 ccaagatccg cgcatggcgt tgacgacgat ggtctacgat ggtgtgtttg ag
//
LOCUS       DASTWE010000234         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_103492, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000234 DASTWE010000000
VERSION     DASTWE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103492"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VFT30_02565"
     CDS             complement(<1..1385)
                     /locus_tag="VFT30_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5091534.1"
                     /translation="MSENTQDIPGLSHAQGKPEIDPICGMTVDPAKAAGQYDHKGTTY
                     YFCGMSCLERFRADPERALSKKPFTAVTMPAPRKPLPMMMHAAPGEIDPVCGMTVQPA
                     TAAGSYDYRGKKYYFCATRCLEKFRADPDYYLTPPEQRVPKPAPVSAGGTVKYICPMD
                     PGVLESKPGSCPICGMALEPADVTAASTRTEYICPMHPEIVQADPGNCPICGMALEPR
                     TVTVEDANPELIDMTRRFWQSVILGSPILSLMISEMMPGRPLQQLFSGRTLVWFQFLL
                     ATPVVLWIGRPLFERAWASIVNRHLNMFTLIGLGTGAAYFYSVAATLVPGLFPDSFRV
                     HGGELAVYFEPAVAIVALVLLGQVLELRARSRTSSALKALLGLAPKTARVVRGDGREE
                     DIPLEQVQVGDPLRVRPGEKIPVDGVVVEGASAVDESMVTGESIPVEKQPGQKVVGAT
                     VNGTGSFVMRA"
     gene            complement(1614..2144)
                     /locus_tag="VFT30_02570"
     CDS             complement(1614..2144)
                     /locus_tag="VFT30_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5091535.1"
                     /translation="MQRLLENESAFRQFVRRRVGDEVVVEDILQQSFTKAVEHSHSLN
                     NKQSALAWFYRILHHTVADYYRSHGAEVRRNEALLQELTISGNHQEPPPDELKTAACA
                     CLYGLLPSLHGNYAELIKRIDLDGELPAQVAKELKISRNNLTVRLHRARQSLRASLED
                     ACGICSRHGCLNCSCG"
     gene            complement(2188..2835)
                     /locus_tag="VFT30_02575"
     CDS             complement(2188..2835)
                     /locus_tag="VFT30_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5091536.1"
                     /translation="MNGRMWKMALLVMGTIGLLNVPVSAQAAKPELKGKFEILTDEPS
                     THQPGKVKVVEFADFYCPHCHHFEETGVPLLLKEFGHKVDITMVGFPVIPGKLPTVFD
                     MYEQAKLMGKGDQMKAILFRTIHKDKVDGVFDRSIRSLLIKEAGLDVAAFEAGMESGK
                     PAKLFEEGRRWGERIKVSSTPALLLDGNIKVDGANMTPENVVTIIRSILEADAKK"
     gene            2923..>3218
                     /locus_tag="VFT30_02580"
     CDS             2923..>3218
                     /locus_tag="VFT30_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU5091537.1"
                     /translation="MPRYACVLSVLVLLSGCASTDYQTDTPSSNTPQISYAQVKSTPE
                     SFNGQPVTFGGKVLAARRLKEGTRIEILQLPLTSSLQPTMDLSKSEGRFVAMQK"
BASE COUNT          733 a          936 c          834 g          715 t
ORIGIN      
        1 tctgcgcgca tcacaaaact tcccgttccg ttgacggtcg ccccaaccac tttctgcccc
       61 ggctgcttct ccacgggaat cgattcgccg gtcaccatcg attcatcgac agcactcgcg
      121 ccttccacca ccactccatc gaccggaatc ttttcgcccg gccgcactcg taacggatca
      181 ccgacctgca cctgttccaa cgggatatct tcttcgcgcc catcaccgcg gacaacgcgg
      241 gctgttttcg gagcgagccc caacagggcc ttcagggcac tgctggttcg actgcgggcc
      301 cgcagctcca gcacctgtcc caataacacc agggccacga tagcgaccgc cggctcaaag
      361 tagacggcaa gctcgccccc atggacacgg aacgagtcgg ggaacagtcc aggaacgagc
      421 gtagcagcga cactgtagaa ataggcagcg ccggtgccga ggccgatcaa ggtaaacata
      481 ttgagatgac gattgacgat tgaagcccag gcccgttcga ataaaggccg accgatccag
      541 agcaccaccg gtgtcgccag cagaaactga aaccagacca atgtccttcc agaaaagagc
      601 tgctgtaaag gtcggcccgg catcatctcg gagatcatca acgaaagaat gggtgaaccg
      661 aggatcacgc tttgccagaa tcggcgagtc atatcgatca actcgggatt ggcgtcctcc
      721 accgtcaccg tgcgcggctc caatgccatc ccacagatcg gacaattccc gggatcagcc
      781 tgcacaatct cagggtgcat aggacagata tattccgtgc gagtactcgc cgctgtcaca
      841 tcggccggct ccaatgccat gccgcaaatg ggacaggacc ccggcttaga ttcgaggacc
      901 ccgggatcca tggggcagat atacttgacg gttccaccgg ccgagacagg tgccggtttc
      961 ggaacacgct gttccggcgg agtgaggtaa tagtcggggt cggcgcggaa cttttccaaa
     1021 catcgagtgg cacagaagta atacttcttc ccccggtagt cgtatgaacc ggccgccgtc
     1081 gccggttgca ctgtcatgcc gcacacaggg tcgatttcac ctggtgcagc atgcatcatc
     1141 atcggcaagg gctttcgtgg agcgggcatc gtgacggcag taaacggctt cttgctcagc
     1201 gcgcgttcgg ggtctgcccg aaatcgttcc aggcaggaca tcccacagaa atagtaggtc
     1261 gtacccttgt ggtcgtattg gcccgccgcc ttggcaggat cgactgtcat accgcagatg
     1321 gggtcgatct caggcttacc ttgagcgtgc gaaagacccg ggatatcttg agtattctcg
     1381 ctcatatggt cgtgtcctgc atgcggcctt ccgcaacagc ccctcaatga ttcgttggtc
     1441 atgcgtcaga ctcctttcat tttatccggt acgcgtagtt attgtcgcgt tcacaattac
     1501 agacggaaga ggccgcaaaa tattacagat cactcgattg acggaggagc gatttgaaaa
     1561 acactcaaaa aatcagaagg aactggtgga aggtgtgtaa gagagtcaga tggctacccg
     1621 caggagcaat tgagacagcc atgtctactg cagatgccgc aagcatcttc caaagaagct
     1681 cgtaaggact gacgggctct gtgcaaacga accgtgagat tgttccgcga tatcttcagc
     1741 tctttcgcga cctgtgccgg caattcgccg tcgagatcga tacgtttgat gagttccgca
     1801 tagttcccat ggaggctcgg aaggagaccg taaaggcagg cgcaagcggc agtctttagc
     1861 tcgtccggtg gcggctcctg atgattgccc gaaatcgtca attcttgcag taaggcctca
     1921 tttcgacgaa cctcggctcc atgagaacga taataatcgg ccaccgtatg gtgaagaata
     1981 cgatagaacc acgcgagcgc gctttgcttg ttattcagcg agtgtgaatg ttcgacggct
     2041 ttggtgaagc tttgttgcag gatatcttcc acgacaactt catcgccgac tcgacggcga
     2101 acaaactgcc tgaaggcgga ctcgttctcc agaagtcgtt gcataacccc actcgttctt
     2161 ccctcctcca ccttagctcc tcatcgatta cttcttcgcg tcagcttcga ggatgctccg
     2221 gatgatggtc acgacattct caggcgtcat attcgcaccg tcgactttga tattcccatc
     2281 gagtaaaagc gccggcgtgg atgacacctt gatgcgctct ccccagcgcc gcccttcttc
     2341 aaacagcttg gccggctttc cactctccat tccggcttcg aacgccgcga cgtccaagcc
     2401 ggcctctttg atcaacaacg aacgtataga gcgatcaaat acaccgtcaa ccttgtcctt
     2461 gtgaatcgtg cggaacaaga tcgccttcat ttgatcgccc tttcccatca gcttcgcttg
     2521 ttcatacata tcgaacacgg tcggaagttt tcccgggatg acggggaatc cgaccatcgt
     2581 gatgtctacc ttgtgcccga attctttcag gaggagcggt acccccgttt cctcgaaatg
     2641 atggcaatgt gggcagtaga agtctgcgaa ttcgacgacc ttcaccttac ccggctgatg
     2701 ggtcgacggc tcatccgtga gtatttcgaa cttgcccttc agctccggct tggccgcttg
     2761 cgcactcacc ggcacattga gcagcccgat cgttcccatc accagaagag ccattttcca
     2821 cattcgacca ttcatttcac gctcctttca caggtaaata gccggtgtct attataacgg
     2881 atggagcgca ttcttgtgct ttgttataat tgcgtcatgg gaatgccgag gtatgcgtgt
     2941 gtcctctctg tattagtact cctttccgga tgtgcatcga ccgattatca aaccgatacg
     3001 ccatcctcca acacgcctca gatcagttat gcgcaggtga aatcgacgcc ggaatcgttc
     3061 aacggacaac cggtcacatt cggaggaaaa gtgttggcgg caaggagact caaggagggg
     3121 acgagaattg aaatcctgca attgccgctg acttcgtccc tccaaccgac aatggatctc
     3181 agcaaatccg aaggccgttt tgtggctatg cagaagga
//
LOCUS       DASTWE010000235         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_264943, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000235 DASTWE010000000
VERSION     DASTWE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264943"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFT30_02585"
     CDS             complement(<1..989)
                     /locus_tag="VFT30_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEU5091538.1"
                     /translation="MSGPLLSLVYTVLVLLLAFLTCLVAPLSSDAQQSDREVYLQQLI
                     DRAEQAKLAEQREWHLLLHYRKGIFGGYESEQDDPRFFMSPNGKTDPSAELASTLAQF
                     FSSELVGRSRQTAQCAFIARYHWLKEQLQFDPTRLPPFSCDRFDRWYEDFEVQSISLI
                     FPSAFLNNPASMFGHTLFRVDQKGQTEQTRILAYTINYAADVPPNAGLAYPIRGIFGS
                     YRGYFSTIPYYLKVQKYRDIENRDIWEYRLNLTENQMRRFLMHAWELGNAYFDYFFFK
                     ENCSYHILALLDYADPELHLTDEFIGWTVPADTVRLIVSKPGLVSDITYRPSR"
     gene            complement(1023..1532)
                     /locus_tag="VFT30_02590"
     CDS             complement(1023..1532)
                     /locus_tag="VFT30_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HEU5091539.1"
                     /translation="MSKKVLLLSIAVLFGAQAGLAMAANPDTGPGCGLGKLAWGEYKG
                     QKEIAPQVLQATTNGTFGSQTFGISFGTSGCTNDGKVMSEHKTTMFASLNFDALSAEM
                     AQGQGEHLASLATLMGVPTERHAAFFAMTQERYTSLVQAGEASPVALVKSLNDAIASH
                     PVLAQASVR"
     gene            complement(1800..>2043)
                     /locus_tag="VFT30_02595"
     CDS             complement(1800..>2043)
                     /locus_tag="VFT30_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091540.1"
                     /translation="IRWTPSQDGATVIYERHGIVERRATIVAAATGYRLIGEDGHLLA
                     EAEYDANGSVRLLDGACRMVNQWSRKELSAIAEASH"
BASE COUNT          529 a          547 c          513 g          454 t
ORIGIN      
        1 ctacgggaag ggcgataggt gatatcggag accaggccag gttttgagac gatcaatcga
       61 acggtatcgg ccgggacagt ccacccgata aactcatccg tcaagtgcaa ttctggatcg
      121 gcataatcca acagcgcaag aatatggtac gagcaattct ccttgaagaa aaaatagtcg
      181 aagtaggcat tccccagttc ccacgcatgc atcaaaaatc gccgcatctg gttttccgtg
      241 agattcaacc gatactccca aatatcccgg ttttcgatat ctcgatattt ctgaactttc
      301 aaataatagg gaatcgttga aaagtagcct cgatagcttc caaagatgcc tcgtataggg
      361 tatgcaaggc cggcattggg aggcacgtcc gctgcatagt tgatggtata ggcaagaatg
      421 cgggtctgct ctgtctggcc cttctgatcg acgcgaaaca gggtatggcc gaacatggaa
      481 gccgggttgt tcagaaaggc tgaaggaaag atcagagaaa tagattggac ttcgaaatcc
      541 tcgtaccacc gatcaaaccg gtcacaggag aacggtggaa gccgggtcgg atcaaactgc
      601 agttgctctt tcagccagtg gtaccgagcg atgaaagcgc actgagctgt ctgccgagag
      661 cgaccaacca gttcagagga gaagaattgt gcaagggtag acgcaagttc agcagacgga
      721 tccgtcttcc cattgggaga catgaaaaat cttggatcgt cctgctcgct ctcatatcca
      781 ccgaaaatcc cctttcggta gtgcaacaag agatgccatt cgcgctgttc cgcaagcttg
      841 gcctgctcgg cccgatcgat gagttgctga agatatacct cacgatcaga ttgctgcgca
      901 tcggaagaaa ggggagcaac taaacaggtt agaaatgcaa gaaggagaac cagcacggtg
      961 taaacaagag acaacaaggg ccctgacatc tctgccaggg cccctataat aaatttaata
     1021 ccttagcgta ccgatgcttg cgcgaggacc ggatggctcg cgatcgcatc attgagggac
     1081 ttcaccaatg caacgggaga agcttccccg gcttgaacca gggacgtata ccgctcctgg
     1141 gtcatggcga agaacgccgc atggcgctcc gttggtacac ccatcagcgt cgcaagcgaa
     1201 gccaaatgtt cgccctgtcc ttgagccatc tcagcgctga gcgcgtcaaa gttcaacgat
     1261 gcgaacatcg tcgtcttgtg ctcgctcata accttgccat cgttggtgca tccggaggtt
     1321 ccaaagctga tgccgaacgt ctggctgcca aaggtgccgt tcgttgtcgc ttgcagcact
     1381 tggggggcga tttccttctg ccccttgtac tctccccagg ccagctttcc aaggccacag
     1441 cccggaccgg tgtctggatt tgccgccatt gccagacccg cctgagcgcc gaacagtacg
     1501 gcgatagaca ataagagcac tttcttcgac ataagtcctc ctccgttaga attagttaag
     1561 cacccatgct actgcaaaaa caccgagacg aagtatagcc gaactcacta tcgatgttta
     1621 caaaatattt tgccactagg gaagtgacta gtgctcgttt ctcaagtgaa aggattatag
     1681 acgaattgca acggagcgca acaacatttt cttggccccg gaattcttac ttacttcaac
     1741 acgcgagtcc agtcaagagg accacagcct acaatttcct tgagtcttcc accaatcatt
     1801 cagtgactag cctcagcgat cgcacttagt tcttttctgc tccattgatt gaccatccgg
     1861 cacgcaccat cgagaaggcg aacgctaccg tttgcatcat attcagcttc cgccagcaaa
     1921 tgtccgtctt caccgatgag acgatacccg gttgcagcag ccacgatcgt cgcgcggcgt
     1981 tcaacgattc cgtggcgctc atagattacc gtcgctccat cttgcgaagg agtccatcgg
     2041 ata
//
LOCUS       DASTWE010000236         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_652891, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000236 DASTWE010000000
VERSION     DASTWE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652891"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFT30_02600"
     CDS             complement(<1..561)
                     /locus_tag="VFT30_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5091541.1"
                     /translation="MSDKATIGSAVLNRLHRLGVRHIFGIPGDYVLSLYQLIEASPIK
                     HIGTTREDCAGFAADAYARINGIGAVCVTYCVGGLNTVNAIACAYAERSPVVLLTGSP
                     GLSERTRTPYLHHMVRDFGTQREVFERMTVAAVTLDDPLTAEREMDRAFAALLRYRRP
                     IYIEIPRDMVHCPLTGGMKPFCIEDTP"
     gene            700..>1664
                     /locus_tag="VFT30_02605"
     CDS             700..>1664
                     /locus_tag="VFT30_02605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU5091542.1"
                     /translation="MGKDMTQSAQVRLTASRGSESRHLWLYAGHLGPVVGEPIAGDLI
                     DVLTSDGRFYGRGLYSPVSKIRVRLLTFRDEPIDETFWLGRIQQTIRLRERVVAQSNA
                     YRLIFGEGDRLPGLIVDRYDQLLVMQTLSVGMDHRKELLGDLLMKELNIAGVYLRNDA
                     KSRSLEGLPLERGFLRGSGPTQVEIYEGSAKFLVDVERGQKTGWFCDQRENRLAAAKF
                     AAGAEVLEVFCHTGAFGIHAALAGARSVESLDGSQDTLAIARQHAVMNQVENCCTYRQ
                     ADAFEEMRHLVKVGRHYDLVILDPPAFARSKQALAGALAGYKDINL"
BASE COUNT          370 a          439 c          497 g          358 t
ORIGIN      
        1 cggtgtatct tcaatgcaga acggtttcat gcctcctgtc agcggacaat ggaccatgtc
       61 cctggggatt tcgatataaa taggacggcg gtagcgcagc agggcggcga aggcccgatc
      121 catctcccgc tctgcggtca ggggatcatc cagcgtcact gccgcaacgg tcattcgttc
      181 aaagacttct cgttgcgtac cgaagtctcg gaccatatga tgcagatagg gggtgcgagt
      241 ccgctcggat agccccggtg acccagttag aagaaccaca ggcgagcgct cggcataggc
      301 gcaggcaatg gcgttgacgg tgttgagccc gccgacacag taggtgacac agacggcccc
      361 gatgccgttg atccgcgcat aggcgtccgc ggcaaacccg gcacaatctt ccctggtcgt
      421 cccgatgtgc ttgatcggcg aagcctctat caattgatag agagaaagaa catagtctcc
      481 cggaatgccg aaaatatgac gaacgccgag gcgatgcagg cgattcaaca ccgctgaacc
      541 aatcgtagct ttgtcgctca tgtgtgtgcg ttcgatagtg actgaatgtt ctggcttcag
      601 gaaaacatac tgacagcagt tcgtcaagaa caacgggcgg tcgcgatatt tctcatccga
      661 cacttgacca ctcactcata gactaaggta caacatggca tgggaaaaga tatgacgcag
      721 tctgcacagg ttcgcctcac tgcctcacgt gggtctgaga gtcgccatct ttggctctat
      781 gccggtcacc tgggaccggt ggtcggtgaa ccgatagccg gcgacctgat cgatgtgctt
      841 acttccgacg gccgattcta cggccgtggc ctctatagcc ccgtctcgaa aatcagagtc
      901 cgcctgctga cgttccggga tgagccaatc gatgagacgt tctggctggg aagaattcaa
      961 cagacgatcc gcttacgaga gcgcgtcgtc gctcagtcca atgcctatcg cctgattttt
     1021 ggagaagggg atcgactgcc ggggttgatc gtcgatcggt atgatcagtt gctggtcatg
     1081 cagactctat ccgttgggat ggatcacaga aaagagctgt tgggcgactt actgatgaag
     1141 gaattgaaca tcgccggcgt ctatctccga aacgacgcca agagccggtc gcttgaagga
     1201 ctgccgcttg aacgaggatt tcttcgcggc agtggcccga cgcaagttga gatttacgaa
     1261 gggtccgcca agtttctcgt cgatgtcgaa cgggggcaga agaccggctg gttttgcgat
     1321 caacgggaga atcggctggc tgcggccaag tttgcggcag gagccgaggt attggaagtg
     1381 ttctgccata ccggcgcatt cggcattcat gcggccctgg ctggagcgcg gtcggtggag
     1441 agtctcgatg gtagtcaaga tacgctggcg attgcccgac agcacgccgt gatgaatcag
     1501 gtggagaatt gctgtaccta tcgccaagcc gatgccttcg aggaaatgcg ccatctggtg
     1561 aaggtcggac ggcactatga tcttgtgatc cttgatcccc cggcctttgc acggagcaaa
     1621 caggcattag ctggtgcgct agctggctac aaagacatca atct
//
LOCUS       DASTWE010000237         1487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_458974, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000237 DASTWE010000000
VERSION     DASTWE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458974"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFT30_02610"
     CDS             complement(<1..337)
                     /locus_tag="VFT30_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091543.1"
                     /translation="MNSNYARALIPLILAATFLLDVFMPWGYAVWVVGDVFGVLLALW
                     IEWPIAPYVVAAIGTVLVQMGHALSFPGIPIDIAVFNRVVGIALLWITAWLVARGRRA
                     KLDDATRRLG"
     gene            complement(762..>1487)
                     /locus_tag="VFT30_02615"
     CDS             complement(762..>1487)
                     /locus_tag="VFT30_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091544.1"
                     /translation="GALYVCCDGDTSWPFITQFFSVAYTSDTMYMVTNKVVFSMELQR
                     VVDAHGLIIAAGIRNGLVELQKYPMHMRLPYHAYRGLFESLPHRILANSDVLARYSEK
                     CGPTLSLSSEKHTLDHEPWFSIVAANDEKLLVDRERFADWPHAEGRLEINPLYKPVSD
                     HGGEGGGIRYRHTFPSSWYEQEDGDCRKYEPESVWISSEILKDLSMGKRTPAMEDLIA
                     HCVIVGVPERFHKPMDPYLSHAA"
BASE COUNT          370 a          434 c          368 g          315 t
ORIGIN      
        1 cccccagtcg cctggtggca tcgtcgagtt tcgcccttct ccctcgcgcc acgagccacg
       61 ccgtaatcca caggagggct atacccacga ctcgattgaa gacagcaatg tctattggga
      121 tgcccggaaa agacagtgca tgtcccattt gcacaagaac ggtcccgatg gccgcaacaa
      181 catacggggc aatcggccac tcaatccaga gggccaataa cacaccgaag acatccccaa
      241 caacccagac tgcataaccc cacggcatga acacgtcgag aaggaacgtc gccgccagga
      301 tcagcggaat cagtgctcgg gcatagttac tgttcattgc agtccgcgat ctgaggaata
      361 tggccgtctg tcattcgatg acagcggcct acagagtaat aagtgacact gccacccggc
      421 cctgaatgat actctaaaat aaggattgag atcgagatgg aggggggaga taaaaaatct
      481 tagttatttg tgcccagccg tttgacgatt gaccacccac acccctgcca agatgaggcc
      541 gataccaatg attttgctca ggctcactgc cagcttgtgc ggtggatcgc ccaccagccg
      601 agacgcgtcg ttgccatcct taatagccgc aggctaggcg gaacacccgc tggcacatca
      661 gctcctggat cgcgtgctgg atcttgtcca gaccgcgatg gtccatcaga cacgccgcca
      721 gcggattcat gtccccgcca tagatagcct gaaccctgac gtcaggccgc gtgagagaga
      781 tatggatcca tcggtttatg aaatcgttcc ggtaccccca cgatcacaca gtgagcaatc
      841 agatcttcca tcgccggtgt acgttttccc atggacaagt ccttcaatat ctcagaggaa
      901 atccatacgc tctccggttc atacttccga cagtctccat cttcttgctc ataccagctc
      961 gaagggaacg tgtgtcggta gcgaattccc ccgccctcac ctccgtggtc gctcactggc
     1021 ttatacaagg ggttgatctc aagccgtccc tcagcatgcg gccagtctgc aaagcgctct
     1081 cgatcgacca agagtttctc gtcattcgct gcaacaattg aaaaccaggg ttcgtggtca
     1141 agcgtgtgtt tctcgctgga tagactgaga gtaggaccac atttctcgct gtatcgggcc
     1201 agcacatcac tattggcaag aattcgatgc ggcaaagatt cgaataaccc gcgataagcg
     1261 tgatagggca gcctcatgtg catcggatat ttctgaagtt cgaccaaccc attgcggata
     1321 cctgcggcaa tgatcagccc atgcgcatcg acaactcgct ggagttccat gctaaagact
     1381 accttattgg tcaccatgta catcgtgtcg gatgtgtagg cgacagagaa gaactgagtg
     1441 atgaacggcc aagacgtatc tccatcacag cacacataaa gtgcgcc
//
LOCUS       DASTWE010000238         1491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1169798, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000238 DASTWE010000000
VERSION     DASTWE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169798"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="rpe"
                     /locus_tag="VFT30_02620"
     CDS             <1..675
                     /gene="rpe"
                     /locus_tag="VFT30_02620"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5091545.1"
                     /translation="SILSADFARLADEVSAVERAGADLIHVDVMDGHFVPNLTVGPPI
                     VESLKKVTKLPLDVHLMITNADAFIPEFAEAGADYLTVHVEACPHLHRTIQSIKERGV
                     KAGVTLNPATPLSLLQEILGDVDLVLIMSVNPGFGGQSFIPSVFKKITEARRMLDQIK
                     SHALLEVDGGVKVENAREIVAAGATVLVAGSAIFSQHDYTATIAAMRAAGETASSPAS
                     RAAVHR"
     gene            685..>1491
                     /locus_tag="VFT30_02625"
     CDS             685..>1491
                     /locus_tag="VFT30_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091546.1"
                     /translation="MDISTLIDSLHPLEIKVLTTLGLRPPGTAQDSERLASAADLEPS
                     QFSMAVEWLLAKSLITVQTETVTPIVSLTKVGEEYYQTASPIERVLAAAREATGTGKR
                     LTIPDLQSQGGFDPSDVSKAVGRLKKEGVVLIIQGGCIETTGRQSPTVEALRTLLGEM
                     HDSPKELKSFTEAHQQVIEDFAVKRGNAKEPFRVDDRVARSFILSPDGAEAAAQLLRQ
                     GIAEEVSQLTPDLLKDGSWRQKRFRKYTISLRPPRIGTGKKHPYREFLDTV"
BASE COUNT          343 a          407 c          430 g          311 t
ORIGIN      
        1 tcgattcttt ccgcggactt cgcgcgattg gccgacgagg tctctgcagt tgagcgagca
       61 ggtgccgacc ttatccatgt cgatgtgatg gacggccatt tcgtgccaaa cctgacggtg
      121 ggaccaccta tcgtcgaaag cctgaaaaaa gtcaccaagc tgccgctcga cgttcatttg
      181 atgatcacca atgccgacgc gttcatccct gagtttgcgg aagccggcgc cgactatctc
      241 accgtccacg ttgaagcctg cccgcacctt catcggacga ttcagtcgat taaggaacgg
      301 ggggtgaagg cgggagtaac cttaaatcct gctactccac tctccttact tcaggagatc
      361 ttgggcgatg tcgatctggt cttgatcatg tcggtgaatc cggggttcgg tgggcagtcc
      421 ttcatcccgt ctgtcttcaa gaaaatcacc gaggcgcgaa ggatgctaga tcagattaag
      481 agccacgcat tgcttgaagt ggacggtggc gtcaaggtgg aaaatgcccg agagatcgtg
      541 gccgctgggg cgacggtcct cgtggccggg tccgcaattt tttcccaaca cgactataca
      601 gccaccattg cagccatgcg ggcggccgga gaaacggcct cgtcgcccgc ctcacgcgct
      661 gcagtccatc gataggcggc tcgggtggac atctccacac tcatcgacag cctgcaccct
      721 ctcgaaatta aagtcctcac gaccttgggg cttcgtccac ctggaactgc ccaggacagc
      781 gaacggctgg caagtgctgc tgatctggag ccttctcaat tcagcatggc ggttgaatgg
      841 ctgttggcga agtcgctcat cacggtgcaa acggaaactg tgactccaat agtatcgctg
      901 acgaaagttg gggaagagta ttaccagact gcttcaccaa tcgagcgagt cttggcggct
      961 gcgcgggaag ccaccggcac cgggaaaagg ctgaccattc ctgaccttca atcacaagga
     1021 gggttcgatc cttccgatgt cagtaaagcc gttgggcggc tcaagaaaga aggagtggtc
     1081 ctaatcatcc aaggaggttg tatcgaaacg accgggcgcc agagtccgac ggttgaggca
     1141 ctccgaaccc tcttgggaga gatgcatgat tcgccgaagg aactgaagag cttcacggaa
     1201 gcccatcaac aggtcattga agactttgcc gtgaaacgtg gcaatgccaa agagccgttc
     1261 cgagtagacg atcgggtggc ccgctcattc atattgtcgc ccgacggtgc cgaggcggcg
     1321 gcacagctgc tgaggcaagg gattgctgaa gaagtatcgc agttgactcc cgacctgtta
     1381 aaggatggga gttggcgtca gaaacgattt agaaagtata cgatcagtct gcgcccgcct
     1441 cgtatcggaa ccggaaagaa acatccctat cgagaatttc tcgataccgt c
//
LOCUS       DASTWE010000239         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1590089, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000239 DASTWE010000000
VERSION     DASTWE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590089"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..869
                     /gene="ccsA"
                     /locus_tag="VFT30_02630"
     CDS             84..869
                     /gene="ccsA"
                     /locus_tag="VFT30_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5091547.1"
                     /translation="MITLVLYLVATVSFLSYSLRRSEALSQVSLGLTAVGFASHTFVL
                     GARMFAASSAASPSFSEALSFFAWMIILVLLVVELRHQIHVLGSFMVPLALVSLVSAA
                     ALPDTEPTLQPVFKTLWFHVTLSMLGAVGFAVAFVAGVMYLIQDRLLKSKQFNVLYSK
                     LPALDFLDHLNQQSIVLGFPLLTLGIVTGAISAQFARGSYVSWNPEQTGALVTWLFYF
                     IVLLGRLTIGWRAKRAAYLTVIGFACVILTLVGVVLKGHSALS"
     gene            888..>1809
                     /gene="hemA"
                     /locus_tag="VFT30_02635"
     CDS             888..>1809
                     /gene="hemA"
                     /locus_tag="VFT30_02635"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU5091548.1"
                     /translation="MHLIVVGLSHKTAPVEIRERLAVPESRLGEALTRLCSYPGIKEG
                     ILLSTCNRVEVYSVVDDIEAGYGRIQEFLADKHLSLSSEQLTPHLYWHTGDRAIGHLF
                     RVAASLDSMIIGESQILGQLKNSFEVALAHKTTGVIMNKVVKKAISVAKRVRTETKIS
                     EMAVSVSYAAVELAKKIFSDLHEKTVLLVGAGEMAKLAARHLIAHGVRHVRITTRTPQ
                     HAVDLADKFGGTAVPFDQFKDDMASADIVLVSTGAAHYLIGAEDVHRAVEERMNRPMF
                     LIDISVPRNIDPAVRHVDNAFLFDIDDLKHR"
BASE COUNT          361 a          473 c          489 g          486 t
ORIGIN      
        1 atcgatataa tttcccactt accttgttga cgttcggtca ttagacactt cagggggcgt
       61 tcccgatggc agcagtctgt ttcatgatca cgctggtcct gtacctcgta gctacagtgt
      121 cgtttctctc ctattcgctg cgacgctcag aagccttgtc tcaggtatcg ttgggtctta
      181 cagccgtggg ctttgcctcc catacgttcg tcctcggagc aagaatgttt gccgcctcat
      241 cagcggcatc gcccagcttt tccgaagcgc tatctttttt cgcgtggatg atcattctcg
      301 tacttctggt ggttgagctc cgccatcaga ttcatgtgct tggctcattc atggtgccct
      361 tggccttggt ttccctggtc tcggccgcgg cccttccgga cacagagccc acacttcagc
      421 cggtgttcaa gactttgtgg tttcacgtca cgctcagcat gctgggcgcg gtgggatttg
      481 ctgtcgcgtt tgtcgcaggt gtgatgtacc tgatccaaga tcgactcctc aagtccaaac
      541 agttcaacgt tctctatagt aagctccctg cgctcgactt tctcgatcac ctcaatcaac
      601 aatctatcgt gttggggttt cctttgctca cgctgggaat cgtgacggga gcgatctcag
      661 cccaatttgc acgcggatcc tatgtgagct ggaatcccga gcaaaccgga gcgctcgtca
      721 cctggctctt ctatttcatc gtattgctcg gaaggctgac tatagggtgg agagccaaac
      781 gtgcggccta tctcacggtc atcggatttg cttgcgtgat cctcacgttg gtcggcgtag
      841 tccttaaagg acatagtgca ttgtcgtaac atgcgactga tagtcccatg catctgatcg
      901 tcgtaggatt aagtcataag acggctccgg tcgagatccg agagaggttg gcggttcccg
      961 aaagccgtct tggcgaggct ctcactcgcc tgtgttcgta tcccggcatc aaggaaggca
     1021 tcctcctctc gacttgtaat cgagtcgagg tctactcggt tgtcgacgac attgaagccg
     1081 gatatggacg tattcaagag tttctagccg ataaacatct gtcgttgtcc tccgaacagt
     1141 tgaccccgca cctctattgg cataccggag atcgagcgat tggtcacttg tttagagtcg
     1201 cagccagtct cgattcaatg attatcgggg aatctcaaat cctgggacaa cttaaaaatt
     1261 cgtttgaagt cgcgctggcc cataagacca ccggcgtgat catgaacaag gtcgtcaaga
     1321 aggctatttc ggtagccaag cgcgtgcgga ccgaaacgaa aatttctgaa atggcggtgt
     1381 cggtcagcta tgccgccgtc gagttggcaa agaagatctt ttcagatctt cacgagaaga
     1441 cggtgctgtt ggtgggtgcc ggagaaatgg cgaagctggc cgcgcggcat ttgatcgctc
     1501 atggcgtgag gcatgtacgt atcacgacga ggactccgca acatgcggtt gatcttgccg
     1561 acaagttcgg tgggacggcg gttccattcg atcagtttaa ggatgatatg gcctcggcgg
     1621 acatcgttct ggtctctacg ggtgcggctc attatctgat cggcgctgag gatgtgcatc
     1681 gcgcggtcga agagcgcatg aaccgtccga tgttcttgat cgatatttcg gttccgcgaa
     1741 acatcgatcc ggccgttcgt catgtcgaca atgcgtttct cttcgacatt gatgatctga
     1801 aacatcgag
//
LOCUS       DASTWE010000240         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_846812, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000240 DASTWE010000000
VERSION     DASTWE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846812"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            582..1121
                     /locus_tag="VFT30_02640"
     CDS             582..1121
                     /locus_tag="VFT30_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091549.1"
                     /translation="MRRLLLALLPLALAGCPTTTAMLVGAPALLSKSEFDERRTETKQ
                     QIEKGLISKEAGEINCRQMLNTVDRNHAAPPPVDPAVCEFGSFADKLYELTRSTESGA
                     LTPELWVTKCKQLAGQPSGKDVCRYDPFAERISQWRRLINEGKASKEGAEMDCRNYVK
                     QVRQHPIPGPEISEDACKL"
BASE COUNT          272 a          277 c          317 g          279 t
ORIGIN      
        1 gtgacgaacg gtgcctcttc aaggaaagcg accgttactg ttgatgttat gactgggagc
       61 caatacttct taattgaccg ctgtaatatt cttgtaccgg aacatgaagt gacatttgat
      121 tctctccgct tggttgatat ctgaggcatt gcgctacgca aagtctgttc cggaaagatt
      181 ttgagagcga gtgcgtggtg agattgtgag ttcgcaggct gatgtttcat tactgatgca
      241 ggtgaggagt agtcaggcat tccccgatat gcaaaaaagg atacacacgt gtatctatgc
      301 tgatactcgt gcctgtgatg ctctctttaa gggtctacct gtacaatgga tcaaatatct
      361 aataatgagg atcgcgtctt gcggtatgac attggatgta tagatttaag aaatcagacc
      421 cgctccttgg gcgagtatga ccttcacgac gtttctcgcg caccatgctt cgtctcgccg
      481 gtcattgttc cacacaaact cttgatctcg atggacgctg tccgtatgat cgcgcacgat
      541 ctacctatgt gatctcttca gtcttgaaag cgaggttctt gatgcgacga ctcctacttg
      601 ccttgttgcc gttagctcta gccggatgtc cgactaccac ggcgatgctg gtcggagccc
      661 cggcgctcct atccaaatcc gagttcgatg agcggcggac ggaaaccaaa cagcagatcg
      721 aaaaggggct gatttcgaaa gaggccggtg aaatcaactg tcgtcaaatg ctgaatacgg
      781 tcgaccggaa tcatgcggcg ccacctcctg tggatcccgc cgtttgtgag ttcgggtcgt
      841 ttgccgacaa gctttatgaa ctgaccaggt ccacggagag cggcgcactg acaccggaac
      901 tctgggtgac caagtgcaag caactggccg ggcagccctc tgggaaagat gtatgccggt
      961 acgacccgtt tgccgagcgg atcagccaat ggcggcgttt aatcaacgaa gggaaggcgt
     1021 cgaaggaagg agctgagatg gactgccgaa actacgtgaa gcaggttcgc caacatccga
     1081 ttcctggtcc ggagatcagt gaggatgcct gcaaactcta ggaatcagtc tggagacaat
     1141 ccgcg
//
LOCUS       DASTWE010000241         1360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_846823, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000241 DASTWE010000000
VERSION     DASTWE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846823"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFT30_02645"
     CDS             complement(<1..322)
                     /locus_tag="VFT30_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU5091550.1"
                     /translation="MTKLKSDDLKSFLEIPYDELEEMNLKAAQRAEKAGVKELEREYT
                     AWLKKETHIKAVTLCFSDIEGRLHMLDYDKKFLLESLGNLTFDGSSIRGFTPQHESDL
                     RLEVD"
     gene            complement(433..678)
                     /locus_tag="VFT30_02650"
     CDS             complement(433..678)
                     /locus_tag="VFT30_02650"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEU5091551.1"
                     /translation="MENQAEDQPVLSYIQRLVEEEHRLHEKRARSKADRKRLSQVQVE
                     LDQCWDLLRQRRALREVGLDPDDAEVRPPRIVENYEE"
     gene            1000..>1360
                     /locus_tag="VFT30_02655"
     CDS             1000..>1360
                     /locus_tag="VFT30_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091552.1"
                     /translation="MPTQTLTDEQLKDQAIRQALAGDAAGARRFANEIVDRRYLREAW
                     QMMLFVESERGNVQAVKDTIVSCPDPSLLASHFYLELPQVFVKAGDRSGAIEIAKAMG
                     DAGVLPLIGIAAHLAEDG"
BASE COUNT          298 a          354 c          369 g          339 t
ORIGIN      
        1 agtcgacttc gagacgcagg tccgattcat gttgcggagt gaacccgcgg atggacgatc
       61 cgtcaaatgt aagattcccc agtgattcca gaaggaactt cttgtcgtaa tcgagcatgt
      121 gaaggcgacc ttcaatatcg gagaagcaca acgtcacggc tttgatgtgc gtttcctttt
      181 taagccaggc ggtatattcc cgttccagct ctttgacccc ggccttctcg gcgcgctgtg
      241 ccgccttgag attcatttct tcaagctcgt catacgggat ctcaagaaaa ctcttcaggt
      301 catcggattt gagcttagtc atggtcttct taaatacttc tttctgaatg tcggaaatga
      361 cccggggccg gacctatata tggtgcgtgt tgggccagct tgcttgctcc ggtactcacc
      421 ccgtctcaat ccctactcct cataattctc gacaatccgt ggagggcgaa cttctgcgtc
      481 gtccgggtca agcccgacct cacggagggc acgtcgctgg cgcaagaggt cccaacattg
      541 atcgagttca acctgaactt gcgagagccg cttccgatcg gctttggagc gtgcccgttt
      601 ctcgtgcaat cgatgttcct cttcgacgag gcgctgaata taactgagga ctggctgatc
      661 ctcggcctga ttttccacca ggtatcctcc ggcgagaaca tgatcggaac ggtcacgttg
      721 tgatgttacg cccacccgaa actacggtca accgatgctc cggccgatgt gtattttgtg
      781 gcgcgtggtg aactgcgttt gtctgcatcg tgcttcaagc gaaacgttcg acgcctatga
      841 gaaaggtgtc atgagctgtc atgccgattg tccggtgtgt agaactgtcg aattagcagt
      901 aaaaagtcga caagaatggt attcaccgct acatttcgaa acaggcgttc tgaaattttc
      961 caatttagta ttcccacgag tccagagagg agccgagaga tgccaactca aaccctcacc
     1021 gatgaacaat tgaaggacca ggcgatcagg caagccctgg caggcgatgc tgccggggcg
     1081 cgacggtttg caaatgagat cgttgacaga cggtacttga gagaggcttg gcaaatgatg
     1141 ctgttcgtcg aatcggagcg ggggaatgta caagccgtaa aagataccat tgtctcatgt
     1201 cctgatccgt cgctcttggc cagtcacttt tatctcgaac tgcctcaagt ctttgtcaag
     1261 gcaggggatc ggtccggagc catcgagatc gctaaggcta tgggtgatgc cggcgtcttg
     1321 ccgctcatcg gcattgccgc ccatctggca gaggatggtg
//
LOCUS       DASTWE010000242         1153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_491333, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000242 DASTWE010000000
VERSION     DASTWE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491333"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1153
                     /locus_tag="VFT30_02660"
     CDS             <1..>1153
                     /locus_tag="VFT30_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5091553.1"
                     /translation="DWPTSTLEMQKNWIGRSIGAEVDFSLADRKGVIRVFTTRPDTLF
                     GATYMVLAPEHPLADVVTSGEQKATVQAYREAAAKKSDLQRQELDKEKTGVFTGGYAV
                     NPVNHERLPIWIADYVLMSYGTGAIMAVPAHDERDWAFARRYHLPIREVISGGNVLQA
                     AFTDTDCGRVVNSASTDGSFSINGLTPSEAIPKITEWLSKQGKGTRAVNYKLRDWLFA
                     RQRYWGEPFPIVWVDGEARPLPEEQLPLVLPETSSFKPSGNGESPLANLDSWLSTIDP
                     ITGKSARRETNTMPQWAGSCWYYLRFIDPKNSTQLVEPAKERYWMPVDLYIGGSEHAV
                     LHLLYARFWHKVLFDIGAASTPEPFLKLVHQGIVLGEDNQKMSKSRGNVV"
BASE COUNT          263 a          304 c          347 g          239 t
ORIGIN      
        1 tgactggccc accagtacat tggagatgca aaaaaattgg atcgggcgat cgatcggtgc
       61 ggaggttgat ttttcactgg ctgatcggaa gggcgtgatt cgagtgttca cgaccagacc
      121 ggacacgctg ttcggtgcga cctacatggt cttggcgcct gaacatccac ttgccgacgt
      181 ggtcacgagt ggggagcaaa aagctaccgt ccaggcttat cgtgaagcgg cggcgaagaa
      241 gagcgatctg caacgacaag aactcgacaa ggagaagacc ggggtcttca ccggcggcta
      301 cgccgtcaac ccggtgaacc atgaacgctt gccgatttgg attgccgatt atgtgctgat
      361 gagttacggg accggcgcga tcatggccgt gccggcgcac gacgaacgag actgggcatt
      421 cgcgcgccgc tatcatctgc ctatccgcga agtgatttca ggcgggaacg tgctgcaagc
      481 ggccttcacc gacacagatt gcggtagagt tgtgaactca gcgagcacgg acggcagttt
      541 ttcgatcaat ggtctcacac catctgaagc gattccgaaa attaccgagt ggttgtcgaa
      601 gcaaggcaag ggaacgaggg cggtcaacta caaactacgc gattggctct tcgcccgaca
      661 gcgatattgg ggcgaaccgt tcccgatcgt atgggtcgat ggagaggcac gtccgcttcc
      721 ggaggagcaa ttgccgctcg tcttaccgga gaccagcagc ttcaagccgt ccggtaacgg
      781 tgagagccca ttggccaact tggattcgtg gctgtcgaca atcgatccta tcaccggtaa
      841 gtcggctcgg cgcgaaacca atacgatgcc tcagtgggcc ggctcttgct ggtactacct
      901 gagattcatt gaccctaaga actcaaccca gctcgttgag cccgcaaaag agcgctattg
      961 gatgcccgtg gatctgtaca tcgggggtag cgagcatgcc gttctgcatc tcctgtatgc
     1021 tcggttctgg cataaagtcc tgttcgatat cggggcggca agtactcccg aaccattcct
     1081 gaagttggtg catcagggca tcgttttggg agaagacaat cagaagatgt cgaaatcgcg
     1141 cggcaacgtc gtc
//
LOCUS       DASTWE010000243         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1008588, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000243 DASTWE010000000
VERSION     DASTWE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008588"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..>1070)
                     /locus_tag="VFT30_02665"
     CDS             complement(219..>1070)
                     /locus_tag="VFT30_02665"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEU5091554.1"
                     /translation="PRSKDYDLVQMDAVKQLYSDARPDLVIHLAARVGGIGANQANPG
                     RFFYDNLMMGTQLIEVGRQRGLKKFVATGTICAYPKFASIPFKEEDIWNGYPEETNAP
                     YGLAKKMMLVQSQAYRQQYGFDSIVLFPVNLYGPRDNFDLETSHVIPALIRKCVAAKE
                     TGQASMTLWGDGSPTREFLYVEDAAEGILLAAEQYDGNLPVNLGTGEEVAIRDLAEML
                     AAEIGFTGKILWDTTKPNGQPRRCLDVSRAKQLFGFEARHSLRDGLKKTVQWFRTNRE
                     SIREVHF"
BASE COUNT          233 a          318 c          262 g          257 t
ORIGIN      
        1 accagcttga cctttcgcgc aagctatcgg ccacgcgagc aggttcgccg ctcggtcgat
       61 agacatactg ttttaagtat gcccctccct cgcgactccg tgtgcgcgcc tcccatagcc
      121 tctcagcaat ttcattatga accgtcatga ataatgcagg ctagtcgagc agaggcaggg
      181 cctgtacaac tgagttccaa ttgaacagcc agaaatatct agaaatgcac ttcgcgtatc
      241 gactcgcggt tggttcgaaa ccactggacg gttttcttca acccatcacg caggctatgt
      301 cgcgcttcaa atccgaagag ctgcttggcc cggctcacgt caagacagcg cctgggctgg
      361 ccgttcggct tggtagtgtc ccacagaatc ttccccgtga acccaatctc agctgcgagc
      421 atttctgcaa gatcacgaat cgcgacctct tcacctgttc caagattgac gggtagattc
      481 ccatcatact gttcggcggc aagcagaatc ccttccgctg catcctccac atacaagaat
      541 tctcgagtcg gcgagccatc cccccacagg gtcatcgacg cttgtcccgt ttcctttgcc
      601 gccacgcatt tccgtatgag tgccggaatg acgtgagacg tttccaaatc gaagttgtcg
      661 cggggcccat agagattcac gggaaataag acaatcgaat caaagccata ttgctgccga
      721 taggcctgcg actggaccaa catcattttc ttcgccagtc catagggggc attggtttcc
      781 tcagggtatc cgttccagat gtcctcttcc ttgaatggaa tggatgcgaa ttttggatag
      841 gcacaaatgg ttccagttgc gacgaacttc ttcaatcccc gttggcggcc gacttcgatg
      901 agctgcgtgc ccatcatcag attgtcatag aagaaccgtc ctgggttggc ctggttggca
      961 ccgatgccgc cgactcgcgc ggccaaatgg atcaccaggt ctggcctcgc gtcgctgtac
     1021 aactgcttca ccgcgtccat ctgaaccaga tcataatcct tgcttcgtgg
//
LOCUS       DASTWE010000244         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_233000, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000244 DASTWE010000000
VERSION     DASTWE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233000"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFT30_02670"
     CDS             <1..913
                     /locus_tag="VFT30_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5091555.1"
                     /translation="AGFRLHGPTCSTETLSSWAADMSRLDLHLHTTHSDGSCTPTEVV
                     NMAHQAAVTALAITDHDIMTGIAESMAAGQQHGIEIIPGVEISSLAGSSEIHILGYFL
                     DWQDFDLLEHLKILRDGRHRRNPMIIERLQTLGIDITYDEVRALARTDSIGRPHIARV
                     LMDKRVVASAKEAFDRFLAEGKPAYVPRELPSPAEAIRWIKAARGLAVLAHPTWVKVS
                     EQPLVDLVRQLKADGLDGVEVYYSTHAARQTREYLSLAQQLGLLVTGGSDFHGLTKPD
                     IEVGIGKGTLHIPTSLLPKMKEAAGRL"
BASE COUNT          250 a          342 c          294 g          252 t
ORIGIN      
        1 ggctggattc cgcctacacg gcccaacttg cagcacagaa acccttagct cctgggctgc
       61 cgacatgagc cgtctggacc ttcatctgca taccacccac tctgatggaa gttgcacgcc
      121 cactgaggtg gtgaacatgg cgcaccaagc agcggtgacg gccctagcga tcaccgatca
      181 cgacatcatg acaggtattg ccgaatccat ggcggccgga caacagcatg gaatcgagat
      241 catacccgga gtggaaatca gctcgcttgc cggcagttcc gagatacata ttcttggata
      301 ctttctagat tggcaggact tcgatttgct ggagcacctg aagatcttgc gagacggtcg
      361 gcatcgcagg aatccaatga tcatcgaacg attacagacc ctggggatcg acattacgta
      421 cgacgaagtc cgcgcactcg cccgcaccga ctcgatcgga agaccgcaca tagcccgtgt
      481 cttaatggac aaacgcgtcg tcgcttctgc caaggaggcc ttcgatcgtt ttcttgccga
      541 gggtaaaccg gcctatgttc ctcgggaact cccgagccct gccgaagcta ttcgctggat
      601 caaagcagct cgcggactgg ccgttctggc ccacccgacg tgggtcaagg tgtctgaaca
      661 gccgctggtt gatctggttc gacagctcaa agccgacggg ctcgacggtg tagaggttta
      721 ctatagtacg catgctgcgc gacaaactcg agaatacctc agtctggctc aacaactcgg
      781 cttattggtc accggtggaa gtgactttca tggcctcacg aaacccgaca tcgaagtcgg
      841 catcgggaag ggtacgctgc acattccgac ctctctgctt cccaagatga aagaagccgc
      901 cgggcgactc tagctcagta tctcccgcat attttcctca atccattgac tcctcttcgg
      961 tatccgctag actctgatta tcttcagatc tcgtcatcca tggtcaacgc gtcgagctgg
     1021 acccttcaat atgtgctggt catactggtt gcactctttg ccggtcccgt actgagcaaa
     1081 ctaccggcgg cgcagtcgtt tcccctgacg ttgttcggcc tgaccggctc tcaaacca
//
LOCUS       DASTWE010000245         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_265097, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000245 DASTWE010000000
VERSION     DASTWE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265097"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFT30_02675"
     CDS             complement(<1..871)
                     /locus_tag="VFT30_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091556.1"
                     /translation="MIQHPDEHRHPTAEFSESLDKPTLFLNRELSWLRFNLRVLEEAE
                     DKRHPLLERVKFLTIFATNLDEFFMIRVSGLRRQVAAGTRGLSPDGMTASEQILAINQ
                     QLIVLAARYDLCWKQDILPSLGEAGIRLLHYAELTQKERETIQQYFHREIFPVLTPLA
                     FDPTHPFPHISSLSFNLAVLVNDPERGDCFARVKVPDIFPRLVPVSADKDAPLQQMGL
                     SGAGKVHQFVWLEEVIAHNLDNLFPGLRVVESYPFRVTRDADVVIEEDEASDLIEAVE
                     EQLDQRDFNSAVRL"
BASE COUNT          257 a          285 c          271 g          239 t
ORIGIN      
        1 ccaatcgtac agctgaattg aaatcccgtt gatccagttg ctcttccacc gcctcgatga
       61 gatcagaggc ttcgtcttcc tcgatcacga catcggcatc tcgcgtcacc cggaatggat
      121 atgattcgac aactcgcaaa ccggggaaga gattatccag gttatgcgca atgacctcct
      181 ccagccagac aaattgatgc actttcccag caccgcttaa tcccatctgc tgtagagggg
      241 cgtccttgtc agcggagacc ggaaccaatc gtgggaagat atccggcact ttgacgcggg
      301 cgaagcaatc cccgcgctcg gggtcattga ccaagacggc aagattaaag ctcagactcg
      361 agatatgcgg aaagggatga gtgggatcaa aggcgagcgg cgtgaggacg ggaaagattt
      421 cgcggtgaaa atactgttga atcgtctctc gttccttctg cgtcagctcc gcataatgca
      481 gcagacgaat gcccgcctct cccaagctgg gcaagatatc ctgtttccag cacaggtcgt
      541 atcgtgcagc aagaacaatc agctgttggt tgattgcaag tatttgctcc gatgctgtca
      601 tgccgtcggg agaaaggccc cgtgtccccg ccgctacctg acgacggaga ccggacacgc
      661 gaatcataaa aaactcgtcc agattcgttg cgaagatggt caaaaacttc acgcgttcca
      721 agagaggatg tcgcttgtcc tcggcctctt ctagaacgcg cagattaaat cgcagccagc
      781 tcagctcacg attcagaaaa agggttggtt tgtcgagcga ctccgagaac tcggcggttg
      841 gatgtcgatg ttcgtcagga tgctggatca tagagcttga ttattgaacg tatgctgccc
      901 cacgatcaat catataatgt tctgtcccga ttcagattgc tacactgttc gggtgggctc
      961 taactccgta acgttgaatc tcgtacgccc tcccattgcc tcgtccgggc acaacttaag
     1021 tagtccatca gccaagcatg tgaaacgcca ct
//
LOCUS       DASTWE010000246         1919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1590183, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000246 DASTWE010000000
VERSION     DASTWE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590183"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..903
                     /locus_tag="VFT30_02680"
     CDS             25..903
                     /locus_tag="VFT30_02680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU5091557.1"
                     /translation="MIKSMTGFGRRQESWSEGTVSVEVRSVNHRFLETAIRVPKSMSG
                     LEERFKKTIQQHCVRGRVDLTVFLQGNRGSARAVQLDVELAKQYYHALRKLQRALKLK
                     GSIDIGLMAGFRDIVVLSEQPTDDPKLIKVVEKLGLLAVSDMTKMREKEGVLLAQDIL
                     ARLDQVREFRGAVLSRAPLIAQETFDRMKQRVEKLLADSIPDLPRLNQELAMYADRCD
                     ITEELVRLDTHMIQFERALQGVEPVGKTLDFLLQEMGREVNTIGSKANDAAIRANVVQ
                     MKAELERMREQIQNIE"
     gene            909..1634
                     /gene="gmk"
                     /locus_tag="VFT30_02685"
     CDS             909..1634
                     /gene="gmk"
                     /locus_tag="VFT30_02685"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5091558.1"
                     /translation="MITPSNHAPGVMGNRQAPERRGILYIISAPSGAGKTTLCKQIVA
                     SVSGLWHSVSFTTRKPRPGEEHGREYFFVDEKVFHDMVARNEFLEYAHVYSNWYGTPR
                     KTLMDKMEHGIDVLLEIDVQGALQIKKKFGDAVYIFILPPSMDILRARLQSRGSDSQE
                     EIARRLQKVKEEVWCFREYYYIVRNDDLTQSLRELQSIFVAERLKTKRMDMHWLEQSF
                     ILEKDTKSAEREPSSTLRGQNLL"
     gene            1640..>1919
                     /locus_tag="VFT30_02690"
     CDS             1640..>1919
                     /locus_tag="VFT30_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU5091559.1"
                     /translation="MLNLLPQYTSNEFDSRHRLVIVASQRAKHLTQGAKQAGSSRFTK
                     ETTIALDEVLRGHAKYLTGKEARDAMKEAKRGKEGETERIAMMTGEDAR"
BASE COUNT          523 a          434 c          546 g          416 t
ORIGIN      
        1 taagtgggaa ggaaaactcc agaaatgatt aagagcatga caggatttgg ccggcgacag
       61 gagtcatggt ccgagggaac cgtcagcgtt gaagtgagat cggtcaacca ccgtttcctt
      121 gaaaccgcca ttcgcgtgcc gaagtcgatg agcggattag aagaacgctt caaaaagacg
      181 atccaacaac attgtgtacg gggaagagtc gatcttacgg tgtttctaca gggcaatcga
      241 ggaagcgctc gtgcggtgca acttgacgtc gaactggcga aacagtacta tcatgctctt
      301 cgcaaactcc agcgtgcgct gaaacttaaa ggctccattg acatcggact gatggcgggg
      361 ttccgcgata tcgtcgtgct ttctgagcaa ccgaccgacg atcctaagct cataaaagtg
      421 gtggagaaac tgggactact cgcggtgtcg gatatgacga agatgcggga gaaagagggg
      481 gtcttgcttg cacaggatat cctggctcga cttgatcaag tgcgtgaatt caggggcgca
      541 gtattatccc gtgcccccct cattgcacag gaaaccttcg atcgtatgaa gcagcgtgtg
      601 gaaaagctgt tggcagactc catcccggac ctccctcggc tcaatcagga attggccatg
      661 tatgccgacc ggtgcgacat tacggaagaa ttggtaagac tagacacgca tatgatacag
      721 tttgagcgtg cgctccaagg cgtcgagccg gtgggtaaga cattggactt tcttcttcag
      781 gagatgggcc gggaagtcaa taccattgga tcgaaggcca acgacgcggc gattagggcc
      841 aacgtggtgc aaatgaaggc cgagctggag cgtatgcgtg aacagataca gaatatcgaa
      901 tgagcaccgt gattactcct agtaaccacg ctccgggtgt tatggggaat cggcaagctc
      961 ctgaacgtcg gggaatcttg tacattattt cagccccttc aggagcaggg aagacgacct
     1021 tgtgtaaaca gatcgtggcg tctgtctccg ggctgtggca ttcggtgtcg tttacgacaa
     1081 gaaaaccgcg cccaggagaa gagcacgggc gtgagtactt ctttgtcgac gagaaagtat
     1141 ttcatgatat ggttgcgaga aatgagttct tggaatacgc acatgtgtat tccaattggt
     1201 acgggacacc acggaagacc ttaatggata agatggagca tggcatcgat gtcttactgg
     1261 aaattgatgt ccaaggtgcg ctccaaataa aaaagaagtt tggcgatgcc gtctatatct
     1321 tcatcctccc tccatcaatg gacattctgc gcgctcgact ccaaagtcgg ggatcggact
     1381 ctcaagagga gatcgcccgc cgattgcaaa aggtgaaaga agaagtgtgg tgtttcagag
     1441 aatattatta catcgttcgc aatgatgatc tgacgcaatc tctccgtgag ctgcaaagca
     1501 tttttgtggc cgaacgtctg aagaccaaac ggatggacat gcattggctt gagcaaagct
     1561 ttattctcga aaaagacaca aagtcggcgg aaagggaacc gtcatccact ttaagggggc
     1621 aaaatctatt atgatcgata tgctgaattt gttaccgcaa tacacgtcaa atgaatttga
     1681 ttcccgtcat cgattggtga tcgtcgcctc acagcgggca aagcacttga ctcaaggtgc
     1741 gaaacaagcc gggtcctcac gcttcacaaa agaaacaacc atcgcactcg acgaggtgtt
     1801 gagaggccat gccaagtatt tgaccggcaa ggaggcccgc gatgcgatga aggaagctaa
     1861 gcgggggaaa gaaggagaaa ccgagcgtat tgcgatgatg acgggagagg acgcccgcg
//
LOCUS       DASTWE010000247         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1008659, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000247 DASTWE010000000
VERSION     DASTWE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008659"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFT30_02695"
     CDS             <1..363
                     /locus_tag="VFT30_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091560.1"
                     /translation="EFDGLMVGLALGWAMSLFSAQDLISGMVLKGTAGFIAGLAGRQV
                     VYLSPVVLVVGLLVVSCLAGLITPFALNLSVQQDWWWAVRTVVLPQACLDAVIGGLIY
                     WLMWSRLNIEQLMSESRM"
     gene            371..>2101
                     /gene="mrdA"
                     /locus_tag="VFT30_02700"
     CDS             371..>2101
                     /gene="mrdA"
                     /locus_tag="VFT30_02700"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5091561.1"
                     /translation="MVTAHYSESEFGDLHRRLFILRVGLLLVVALLGLRLWHLQIYEG
                     PYYRDLSENNRTRLVLLEPARGLVYDRHGVLLANNVPSFSLYVTLEDVKDREVLIQQL
                     TDLLGLDPTIVRKKMTTKGSKLLPRKIKDRMTLRDATLVESHRLDMPGVMIQVESQRN
                     YPGGVTAAHLLGYVGEISADQLEKPEFLDLHQGSIVGQYGVEKSYDRHMRGMAGQKSV
                     EVDALGHEKKASVVERPQAGNDLYLTIDVRLQKVAEDLLGTEQGAIVALDPNTGDILA
                     MASRPGFDPNVLSRELTAKQWVEIVQDEGRPLNNRASQGQYPPGSTFKIPMAVAALET
                     KTMSPSSTVFCNGGYQFGKRLYHDWKASGHGYVDLHNALVHSCDVYFYTIGQRMGIDV
                     MAEFGRDFGLGKATGVELPSERSGIMPSTAWKQKAKNEQWLPGETISAAIGQGYVTVT
                     PLQMASLVGTVANDGVSYRPRLVQAVMDRMSGNLQELPAVPRGKINAKPETFRVIKEA
                     LAEVVTKGTATKAKSSLVTIGGKTGTAQVAALRTGPEENIPKKFRDHAWFVAFAPVES
                     PKIAVAVLAEH"
BASE COUNT          456 a          544 c          644 g          457 t
ORIGIN      
        1 gagtttgacg gcttgatggt cggtctcgcg ctcgggtggg caatgagtct gttctctgca
       61 caagacctga tttcgggtat ggttttgaag ggaaccgcgg ggttcatagc aggcctcgcg
      121 ggccggcagg tggtctacct gagccctgtc gtccttgtgg tgggactgct ggtcgtctcc
      181 tgcttggctg ggctgatcac tccgttcgcc ctcaatctca gtgtgcaaca ggattggtgg
      241 tgggctgtcc ggacagtagt gctgccgcaa gcctgccttg atgcagtgat tggaggactg
      301 atctattggc tcatgtggag tcgtttgaat atcgagcagt tgatgtccga gtctcgaatg
      361 taggcttcgt atggtgacgg cgcattattc ggaatcagaa tttggcgacc ttcatcgacg
      421 gcttttcatt ctccgtgtag gactcttgct ggtggtagcg ctgctgggtc tgcgtctctg
      481 gcaccttcaa atttacgaag gaccgtatta tcgagacttg tcggaaaaca atcgtacgag
      541 attggtgctg ctcgaaccgg cgcggggcct ggtctacgac cggcacggtg ttctcctagc
      601 gaataacgta ccgagcttca gtctctatgt cacgctggaa gacgtgaagg accgcgaggt
      661 cttgattcag cagctgaccg atttgctcgg cctcgacccg accatcgttc gaaaaaaaat
      721 gaccacgaaa ggcagcaagc tgcttccgcg caagatcaaa gaccgcatga ccctgcgcga
      781 tgccacgctg gtcgaatccc atcgtctcga catgcccgga gtcatgattc aggtcgagtc
      841 gcagcgcaac tatccgggcg gcgtgacagc ggcccatctc ctgggctatg taggagaaat
      901 ttcagcggat caactggaga aaccggagtt tctcgatctc caccaaggca gcatcgtggg
      961 gcaatatggt gtggaaaagt cctatgatcg gcacatgcgg ggaatggccg gtcaaaagag
     1021 cgtggaagtc gatgcgctcg gccacgagaa gaaagcgtcg gttgtcgaga ggccgcaagc
     1081 ggggaacgac ctctatctca ccattgatgt gcgactccaa aaggttgccg aagatctgtt
     1141 gggcacagaa cagggcgcca tcgtcgctct tgatccgaac accggtgata ttctagccat
     1201 ggccagccgt cccggcttcg acccaaacgt gctctcgcga gaactgaccg cgaagcaatg
     1261 ggtggaaatc gtgcaggacg aagggcgtcc gttgaacaat cgtgcctcac aggggcagta
     1321 tcctcccggt tccaccttca agatcccaat ggccgtcgct gcgttggaga caaaaaccat
     1381 gtcgccttcc agtaccgtgt tctgcaatgg gggataccag ttcggcaagc ggctgtatca
     1441 tgattggaaa gccagcggtc atgggtatgt cgatctgcac aatgcattgg tccattcctg
     1501 cgacgtgtac ttttatacca tcggtcaacg gatggggatc gatgtgatgg ccgagtttgg
     1561 gagagacttc ggactgggga aggctactgg agtggaatta ccgtctgaac gatccggtat
     1621 catgccttca accgcatgga agcaaaaagc caagaacgag cagtggttgc ccggagagac
     1681 catttcggcc gccatcggac aggggtatgt gaccgtgacg ccgttgcaaa tggcgagtct
     1741 cgtcggcaca gtggccaatg atggagtcag ctaccgccct cgtctggtgc aggcggtcat
     1801 ggatcgaatg tcgggaaacc ttcaagaatt gccggccgtt ccgcgcggaa aaatcaatgc
     1861 gaaaccggaa accttccgcg tcatcaagga ggcgctcgcc gaggttgtta caaagggaac
     1921 ggcgacgaag gcgaaatctt ccctggtgac gatcggtggg aagacaggca cggctcaagt
     1981 ggcggcgctt cggactggac cggaagaaaa cattcccaaa aagttccgag accatgcgtg
     2041 gttcgtcgcc tttgcgcctg tggaatcgcc gaagatcgcc gtcgccgtgc tcgctgagca
     2101 t
//
LOCUS       DASTWE010000248         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_361974, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000248 DASTWE010000000
VERSION     DASTWE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361974"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="groEL"
                     /locus_tag="VFT30_02705"
     CDS             complement(<1..779)
                     /gene="groEL"
                     /locus_tag="VFT30_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5091562.1"
                     /translation="MAAKIVKFNRDARDAILRGVNILADAVTVTLGPKGRNVVLDKSF
                     GAPNVTKDGVTVAKEVELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLARQIFAE
                     GVKLVAAGHDPMTLKRGIDKAVSAIIAELKTLSKPTKDPKEIAQVGTIAANNDSTIGA
                     VISEAMNKVGKEGVITVEEAKGLETTLNVVEGMQFDRGYLSPYFVTDPERMECVLEDA
                     YLLIHEKKISTMKDILPILEQIAKSGKPFIIIAEDIEGEALA"
     gene            complement(807..1082)
                     /gene="groES"
                     /locus_tag="VFT30_02710"
     CDS             complement(807..1082)
                     /gene="groES"
                     /locus_tag="VFT30_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5091563.1"
                     /translation="MQDRVIVKRIEEEEKSKGGIIIPDTAKEKPQEGKVIAVGKGKIS
                     DDGKITPLDVKVNDRILFGKYSGTEINVDGEEHLIMREDDILGVIEK"
BASE COUNT          192 a          302 c          281 g          309 t
ORIGIN      
        1 gccaaggcct cgccttcgat gtcttcggcg atgatgatga aaggcttgcc ggatttggcg
       61 atttgctcga gaatcggcag gatgtctttc atggtgctga tctttttttc gtggattagc
      121 aaatatgcgt cttcgagcac gcattccatt ctctcgggat cagtcacaaa gtacggggag
      181 aggtagccac gatcgaactg catgccctcg acgacattga gtgtcgtctc caagcctttt
      241 gcttcctcga ccgtgatgac gccctccttg cccactttgt tcatcgcttc ggagatcacc
      301 gcaccgatgg tggagtcgtt attggcggcg atggtgccga cttgggcgat ctctttggga
      361 tccttggttg gcttcgacag cgtcttgagt tcggcgataa tcgccgacac agccttatcg
      421 atgccacgct tgagggtcat tggatcgtgg ccggcggcca ccagtttgac gccttcggcg
      481 aaaatctggc gggccaatac ggtcgccgtc gtggtgccgt cgccagcgac gtcagaagtc
      541 ttgctggcca cctctttgac catctgcgcg cccatgtttt caaacttgtc ttcaagttcg
      601 acttccttgg cgacggtaac gccgtctttg gtgacgttcg gagcgccgaa ggatttgtcc
      661 aacacgacgt tgcggccttt cgggcctagg gtcaccgtca ctgcatcggc gagaatgttc
      721 acgcctctta ggatcgcgtc acgcgcgtcg cgattaaatt tgactatttt agctgccatc
      781 gtttcctcct cagattatct gcttaattac ttttcgataa cgccgaggat gtcgtcctcg
      841 cgcatgatca gatgctcttc gccatcaacg ttgatttccg tgccggaata ttttccaaac
      901 aggatgcggt cattcacttt gacatcgaga ggggtaatct tgccgtcgtc gcttatcttg
      961 ccttttccga cggcaatcac tttcccttcc tgcggctttt ctttagccgt gtcggggatg
     1021 atgatgccac ccttactttt ttcctcctcc tcaatgcgtt taacaatgac ccggtcctgc
     1081 aaag
//
LOCUS       DASTWE010000249         1216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_879202, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000249 DASTWE010000000
VERSION     DASTWE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879202"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..405)
                     /locus_tag="VFT30_02715"
     CDS             complement(13..405)
                     /locus_tag="VFT30_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091564.1"
                     /translation="MHQQPIVIFVCEHGAAKSILAAAFFNKLARENDAGFKAMARGTH
                     PDAELSSKTVSGLREDGLASTESVPLRLTREEMESAQRVVSFCTLPEEYGHEVVVEYW
                     DDVPPVNEDYERARDAIVEHLKELIRTL"
     gene            complement(488..928)
                     /locus_tag="VFT30_02720"
     CDS             complement(488..928)
                     /locus_tag="VFT30_02720"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEU5091565.1"
                     /translation="MKWVTWKNIGVDRMASIWLIRRWIDPTAEFNFISVGETPLPEHG
                     EPFDIPGARYSHHGGHCTFHALLKTQKMQDPILKRIAQMVDEADEVQEVTLEPAAWGL
                     DLICRGLRQISRDDFEALERGSLIYDALYAQLASDWVPTEPDGK"
     gene            complement(925..>1216)
                     /locus_tag="VFT30_02725"
     CDS             complement(925..>1216)
                     /locus_tag="VFT30_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091566.1"
                     /translation="AEQIQWLAAEIDEMGGNAFYWRANSLLRAQEDSIVRQFQEQVDA
                     VYSSLLRQLEKSKPDLQEISREYQQALSQDFFQSKLGMLVREKLTSKRGEQS"
BASE COUNT          266 a          351 c          292 g          307 t
ORIGIN      
        1 cgggctgtat attcataaag ttctgatcag ttctttgaga tgctcgacga tggcatcccg
       61 ggccctttca taatcttcgt tgaccggcgg cacgtcgtcc cagtattcta caacgacttc
      121 atgaccatat tcctccggca atgtgcaaaa gctaacaacc cgttgtgccg attccatctc
      181 ctcccgggtt aatcttaagg gtaccgattc cgtggaagca agcccgtctt cccgcaagcc
      241 agagactgtt ttggatgaca attccgcatc gggatgggtg ccccgcgcca tcgccttaaa
      301 ccccgcatcg ttctcgcgtg ccagtttatt gaagaatgca gcggcaagga tgcttttcgc
      361 cgcgccgtgc tcgcaaacaa aaataacgat gggctgttga tgcatttttc ctctcgtttg
      421 cttctaacga tctgcagaaa gatccgtgtc tggcttcggc gttaaccgaa ggatggtgac
      481 aggttgttta ctttccatcc ggctccgtgg ggacccaatc ggatgccaac tgcgcataaa
      541 gagcatcata gatcaggctg ccccgctcca acgcctcgaa atcatcccgg ctaatttgtc
      601 tcagaccgcg gcagatcagg tcaagccccc aggcggcggg ttctagcgtg acttcctgca
      661 cctcatcggc ttcatccacc atctgcgcga tccgcttcag gatcggatcc tgcatcttct
      721 gcgtcttgag aagggcatgg aatgtacaat gtccaccgtg atgcgaataa cgggcaccgg
      781 gaatatcaaa gggttctccg tgctcaggca gtggcgtctc gcccacagag ataaagttga
      841 attcagcagt tggatcaatc cacctgcgga tcagccagat gcttgccata cggtcgacac
      901 cgatattttt ccatgtgacc catttcatga ttgttctcca cgctttgacg taagtttttc
      961 ccgcacgagc atccccagtt tcgactgaaa gaaatcctgg gaaagcgctt gttgatactc
     1021 ccgcgatatc tcctgaagat caggtttcga tttttcaagc tgcctcaaaa gactggaata
     1081 caccgcgtcc acctgctctt gaaattggcg gacgatggaa tcttcctgtg cccgcagaag
     1141 gctgttcgct cgccagtaga aggcattccc acccatctca tcgatctccg cagctagcca
     1201 ttggatctgt tccgcg
//
LOCUS       DASTWE010000250         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1460953, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000250 DASTWE010000000
VERSION     DASTWE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460953"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..>1510)
                     /locus_tag="VFT30_02730"
     CDS             complement(333..>1510)
                     /locus_tag="VFT30_02730"
                     /inference="COORDINATES: protein motif:HMM:NF041640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HEU5091567.1"
                     /translation="TPAVAHGERSQEPFLRMRTVNWYDTEWVGKSTKVNDVTELRGKF
                     HLSEDWPRAVVKPNRTFLNIGSPSSVFVRLSSKINGTPMFVAGPMEVGRDYEYVVVLK
                     ARLPGHHHIHPMFAVKDAGPIAGPGGWMDITGRYEDFTNPIKTLTGETFDSETMGTAT
                     GIMWHLFWAAVAIFWVGFFMIRPMYLVRARVLAAYGDEILLDPIDRKVGAAVLIFVLV
                     VVTVGYLAADARHPVSVPLQAGETKVKPMPIKPNPFVVEVTHAEYDVPGRALRMTLHA
                     TNNGTEPVTIGEFTTAGIRFTNKMGATKLDPNYPAELVAQAGLTMDNEAPIQPGQTVD
                     IKIESKDVLWEVQRLVDILHDPDQRFAGLLMSWTEKGDRLINPIWAPVLPVFTRLGA"
BASE COUNT          362 a          417 c          413 g          318 t
ORIGIN      
        1 agcgaaggaa cgaggcatgc gacggagttg catcgtgacc tctcgatgag gcccgttgta
       61 gcaaattccg tctcacatgt atctgacccg cttcagttgt ggcaaggggg cgagtctatt
      121 cttaagaaag aaattatagg aggtcaagag tggtcgattc gtactatgtg tccaaattga
      181 ggatgcagat aaacctaaga catccatggg gaggagaagg tcatgcaagg gtgagagcga
      241 gaccaatcga tagaaggaaa aaaacaaaaa cgccgacccg ctgagccatc tttcgatggg
      301 gcgggtcggc gtcttgttag aggcgcgttg ctctaggctc ccagtcttgt gaagacgggg
      361 agaaccggag cccagatcgg attgatgagc cgatcaccct tctctgtcca ggacatcaac
      421 aatcctgcga agcgctgatc cggatcgtgg agaatatcca ccaaccgctg cacttcccac
      481 agaacgtcct tggactcgat cttgatgtcg acagtctgac caggttggat cggggcttcg
      541 ttatccatcg tcaaaccggc ctgagcgacc agctctgctg gatagtttgg atcgagtttg
      601 gtagccccca tcttgttggt aaaccggatg cctgcggtcg tgaattcacc aatggtcaca
      661 ggctcggtac cattatttgt cgcatgaaga gtcatgcgta aggctcgccc tggaacgtcg
      721 tattcagcat gagtgacttc gactacgaac gggttcggct tgatcggcat cggcttgacc
      781 tttgtctcac cggcctggag cggcacgctc acagggtgcc gtgcatctgc cgccagatac
      841 ccgacggtca caacgaccaa gacgaagatc aagaccgcag ctccgacctt ccgatcgatg
      901 gggtcaagca gaatctcatc cccatacgct gccagcacgc gagcccggac caggtacatc
      961 ggccggatca tgaagaagcc gacccagaag atggccacgg ccgcccagaa taggtgccac
     1021 atgattccag tggccgtacc catggtttcc gaatcgaacg tctcaccggt cagtgtcttg
     1081 atcggattgg tgaagtcctc atatcgacca gtgatatcca tccatccacc agggcctgca
     1141 atcggacccg catccttgac ggcaaacatt ggatgaatgt gatggtgacc ggggagtctg
     1201 gccttcaaga caaccacata ttcataatct cgaccaactt ccatgggacc cgcaacaaac
     1261 atgggcgttc catttatctt cgagctgaga cgcacgaaga ccgaactggg agaaccgatg
     1321 ttgaggaacg tccggttcgg tttgacaacc gcgcgcggcc agtcctctga cagatggaac
     1381 ttacccctca gctctgtcac atcattgacc ttggtcgact tcccaaccca ttcagtgtca
     1441 taccaattca cggtgcgcat ccgcaggaac ggctcctgcg agcgctctcc gtgagcgact
     1501 gcaggggtga
//
LOCUS       DASTWE010000251         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_976314, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000251 DASTWE010000000
VERSION     DASTWE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976314"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..604)
                     /gene="tsaA"
                     /locus_tag="VFT30_02735"
     CDS             complement(179..604)
                     /gene="tsaA"
                     /locus_tag="VFT30_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEU5091568.1"
                     /translation="MRSPVYTIEPIGFIRSELTQLEDAPMQGDEGAPDAWLELTPSAA
                     PGLLGIQPGNELIVLTWLHLAERDVLQVHPRGDINRPLPGVFATRSPDRPNPIGLHLV
                     SVLEVADVDRKLRVAPLEAIDGTPIVDIKPVLAGSASDR"
     gene            687..>1149
                     /locus_tag="VFT30_02740"
     CDS             687..>1149
                     /locus_tag="VFT30_02740"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEU5091569.1"
                     /translation="MILITGAGGKTGRALIKTLSKVESLSAFVHRPEHAPVVKALGAE
                     KVIVGDLRDESAVRSAMVGASAVYHVCPNMNPDEIVIGTLVIRGARKMNVDHFVYHSV
                     LHPQTEKMKHHWQKLHVEEMIFESGLPFTILQPAPYMQNLLTGWKNIIKDGI"
BASE COUNT          281 a          280 c          317 g          271 t
ORIGIN      
        1 ttcagaataa acctctccaa cttaaagagg ggagtcgtat agaacgcaaa gataaattca
       61 gggaaagata cttgcccggg agcgtctgtc tggtagcagt ctacataggc tcccattgtg
      121 gaatatttat ccagcattgt attggctggg accgggcatt tcttgaccga gagcttcatt
      181 agcggtcaga tgccgagccc gcaagtacgg gttttatgtc cacgatgggc gtcccatcga
      241 tcgcctccag cggcgcgacc cgcaatttcc gatccacgtc cgcgacttcc aggaccgaga
      301 cgagatggag cccgatgggg tttggacgat cgggtgaacg cgtcgcgaac acgcctggta
      361 gcggtctgtt gatgtcgccg cgcggatgca cctgcaatac atcacgctcg gcgaggtgaa
      421 gccacgtcag cacgatgagc tcgttgccgg gttgaatgcc cagtaacccg ggcgctgccg
      481 agggtgtgag ttccagccag gcgtctggtg cgccttcatc cccctgcatg ggcgcgtcct
      541 cgagctgtgt gagctcggag cggataaatc caattggttc aatcgtataa acgggtgatc
      601 gcatttgaaa tcctttttac ctctaaagca atggcgcatt tcataatcaa cttaacaagt
      661 ctttataatg cattatattc agaatcatga ttctaatcac aggcgccggc ggcaaaacgg
      721 gtagagctct cattaaaacg ctctcgaagg tggagagcct ttctgcgttt gtgcatcgcc
      781 cggaacatgc tccggttgtg aaagccctgg gggcagaaaa ggtgatcgtt ggagatctgc
      841 gcgatgaatc agcggtccgt tcggcgatgg tgggagctag cgcagtctat catgtgtgtc
      901 caaatatgaa tccggatgag atagtaattg gcacgttggt cattcgtggg gctcgcaaga
      961 tgaatgtaga tcatttcgtc taccattccg tcctgcatcc gcaaacggag aagatgaagc
     1021 atcactggca aaaactacat gtggaagaga tgatcttcga gtcaggattg ccattcacaa
     1081 ttctacagcc tgcgccgtat atgcaaaatc tcctgacagg ctggaaaaac atcatcaaag
     1141 atggaattt
//
LOCUS       DASTWE010000252         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1234692, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000252 DASTWE010000000
VERSION     DASTWE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234692"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VFT30_02745"
     CDS             complement(<1..1111)
                     /locus_tag="VFT30_02745"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5091570.1"
                     /translation="MGRRTFIRWWGLKLFLCTGLVTALYGQAPSSGNGIPSHPRELKY
                     PKLTYTPPKAVTYRRVLANGVVAFLAEDHDFPLVNVSVLVRTGSYLDPKGKEGLGGMM
                     GNQLRAGGTTSLPPDKFDEEVDFLAATINSGVGDTQGTANLNCLSKDIDKALELFVSM
                     LKAPGFQQDRIDLYKNQALQGMERRNDKTEGIQAREWGRLMRGDQHFSTLPTTKSSIE
                     SITRQDLIDFHKKYYHPGGFILAVSGDFKTSEMISRLEKMFQGWPTLTTSVPAVPKPA
                     HMPQSGVYMVNKSDVNQGRVSIGHLGTMRDNPDYYALNIMDDILGGGGFTSRIMSRVR
                     SDEGLAYSAGSNYGFGVYYDGVFQASFQSKSPTVAQ"
BASE COUNT          235 a          314 c          314 g          268 t
ORIGIN      
        1 cttgcgcaac ggtcggactc ttcgactgaa aactcgcctg gaaaacgcca tcgtaataaa
       61 cgccaaagcc atagttggag ccggcgctgt acgcgagacc ttcgtcggaa cgcacgcgcg
      121 acatgatgcg cgaggtaaat ccgccgccac ccagaatgtc gtccatgatg ttcagcgcat
      181 agtaatccgg gttatcgcgc atcgtcccga gatggcctat cgagacgcga ccctggttga
      241 cgtcgctctt gttcaccatg tagacgcccg actgaggcat atgcgcaggc ttggggactg
      301 ctggcacgct cgtcgtgaga gtgggccagc cctggaacat cttctccaag cgcgaaatca
      361 tttcagaagt tttgaaatcg cccgaaactg ccaggatgaa tcctcccggg tgatagtact
      421 tcttgtgaaa atcgatcaaa tcctgccggg tgatggattc gatggatgac tttgttgttg
      481 ggagtgtcga aaagtgctgg tcacctcgca tcagccgtcc ccattcacgc gcttgaatgc
      541 cctccgtctt gtcgttgcgg cgctccatgc cctggagcgc ctggttcttg taaagatcga
      601 tgcggtcctg ctgaaagcca ggggccttga gcatgctgac aaatagttcc agtgctttgt
      661 cgatgtcttt gctcaggcaa ttgagattcg ccgtgccctg cgtgtctccc actccgctat
      721 tgatcgtcgc agccagaaaa tccacttcct cgtcaaactt atcgggtggt agtgaggttg
      781 tgcctcctgc ccgaagctgg tttcccatca tgccgccgag tccttccttt cccttcgggt
      841 ccagatagct gccagttcga acaagcacgg atacgttgac gaggggaaaa tcatggtctt
      901 ctgccaggaa agccacgacg ccgttggcaa ggacccggcg ataagttacc gccttcggag
      961 gagtgtaggt aagcttggga tatttgagtt cgcgtgggtg cgagggaatg ccgtttccac
     1021 tggatggagc ctggccataa agtgccgtca caagtcctgt gcagaggaag agtttgaggc
     1081 cccaccatcg aatgaatgtt cgtcgcccca tcgttatttc cctccctcaa g
//
LOCUS       DASTWE010000253         1285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_135955, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000253 DASTWE010000000
VERSION     DASTWE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135955"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="purB"
                     /locus_tag="VFT30_02750"
     CDS             <1..847
                     /gene="purB"
                     /locus_tag="VFT30_02750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5091571.1"
                     /translation="KRLWQVRNEIAVGKLSGAMGTFAHQGPDIEDYVCAKLGLKADPV
                     SNQVVQRDRHASYVASLALLAASIEKFATEIRHLQRTEVLEAEEFFSEGQKGSSAMPH
                     KRNPIVSENLCGLARLVRANSVAAMENVALWHERDISHSSVERVIMSDSTILIDYMLA
                     KVTDLIKHLVIYPDRMRRNLELTGGLVYSQRLLLALVEKGAQRKESYEAVQRNAMASW
                     RGGGELQELAGKDPFISQHLTKSEIAACFNPKYYLRHLDQIYRRVFGRHRQQMTGTSG
                     RRGRS"
     gene            844..>1285
                     /locus_tag="VFT30_02755"
     CDS             844..>1285
                     /locus_tag="VFT30_02755"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU5091572.1"
                     /translation="MTRLHAATLALFSVMVGLWLSPAYAGADREKLLNDPGVYGTFAV
                     FAFDEEWAKQDPATRILRLTSLKGVVEQHREHVVIDLYLLRGLSDQADLLFRIHAAEL
                     RGTQEFLLDLKSSQFGKHLKTVGIMHGLTKKPNYVPGFPDQMKTD"
BASE COUNT          291 a          327 c          382 g          285 t
ORIGIN      
        1 aaagcgttta tggcaggtgc ggaatgagat tgccgtcggc aagctgtccg gcgccatggg
       61 aacctttgcg caccaggggc ctgatattga agactatgtc tgtgccaagc ttggcttgaa
      121 ggcagatccc gtctcgaacc aggtcgtcca acgggaccgc cacgcttcct atgtcgcgtc
      181 ccttgcgctg cttgccgcga gcatcgagaa gtttgccacc gagattcgtc atcttcaacg
      241 cactgaagtg ctcgaagccg aagagttttt ctccgaaggg caaaaaggat cgtcggcgat
      301 gcctcacaaa cggaatccga ttgtctcgga gaatctctgt ggcctggcgc ggctcgtgcg
      361 ggccaacagt gtggcggcga tggaaaacgt cgcactctgg catgagcgcg atatcagcca
      421 ttcatcggtc gagcgggtga tcatgtcgga cagcacgatt ttgatcgact acatgctcgc
      481 caaggttact gatctgatca agcacttggt catctatccc gatcggatga ggcgaaactt
      541 ggaattgacc ggggggctcg tgtattcgca gcggctgctg ttggccttgg ttgagaaagg
      601 ggcgcagcga aaagagtcat acgaagcggt tcaacgcaat gccatggcct cttggagagg
      661 aggtggcgaa ttacaggaat tggccggtaa agaccctttt atttcacagc atctcacaaa
      721 gagtgaaatt gcggcctgct tcaacccaaa atattacctg cgtcatctcg atcagatcta
      781 tcggcgggtg ttcggtcgcc acaggcaaca gatgaccggc acaagcggga ggagaggtag
      841 atcatgacac gattacacgc tgcgacactg gcgcttttct ccgtgatggt cggtctttgg
      901 ctatctcctg cctatgccgg ggcagatcga gagaaacttc ttaacgatcc aggagtctac
      961 ggcacgtttg ccgtcttcgc gttcgatgag gagtgggcga agcaagaccc cgcgacgcgg
     1021 atcctccgtc tgacgtcgct caaaggtgtg gtggaacagc atcgggagca tgtggtaatc
     1081 gatctgtatt tgctgcgagg gctttccgat caggccgacc tcttgttccg gattcatgcg
     1141 gcagaattgc gcgggacgca ggagtttctc ctggatctga aaagcagcca attcgggaaa
     1201 cacctcaaga cggtcgggat catgcatggg ctcaccaaaa aaccgaatta tgtgccggga
     1261 tttccggatc agatgaagac tgact
//
LOCUS       DASTWE010000254         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1525610, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000254 DASTWE010000000
VERSION     DASTWE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525610"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..333)
                     /locus_tag="VFT30_02760"
     CDS             complement(139..333)
                     /locus_tag="VFT30_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091573.1"
                     /translation="MVTTHEMRIFALDCLRWAEHTSNPSDRETMVRVARMWMNTASGI
                     DRRINDGWEFACPDLRTKLD"
     gene            complement(336..581)
                     /locus_tag="VFT30_02765"
     CDS             complement(336..581)
                     /locus_tag="VFT30_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091574.1"
                     /translation="MLDLVTLDHDRVYPPEIVVAMSAAFDNVCRSVSPKINGDPRVRR
                     QLALIILRHVDQGERDPERLSELALNELAGIDRPTVG"
     gene            complement(692..>1113)
                     /locus_tag="VFT30_02770"
     CDS             complement(692..>1113)
                     /locus_tag="VFT30_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5091575.1"
                     /translation="TAAAMTESVIARIDKTTMLKTLREEPKFSEMFTSYLLARTIRVE
                     EDLVDQLFNSSEKRLARALLILSKVGQEGKPEPIVPGVDQATLAEMIGTTRSRVSFFM
                     NRFRELGLIEYNGDLKVHSSLLDVVLHEKPPHIQGSR"
BASE COUNT          248 a          335 c          285 g          245 t
ORIGIN      
        1 aggtcagcat cgatgtccgc tcgaaaatca ttgtccatga cctgtccgga gaaagttccc
       61 tactcccgac agagtttatt cgagattttt ggttgtccaa ccctatctat tccgccccaa
      121 gtgttggctg gccttcaatc aatcgagctt cgtccgaaga tcgggacagg cgaactccca
      181 accatcattg atacgtcgat cgatgccaga ggcagtattc atccacatgc gcgcgacgcg
      241 gaccatcgtc tcccggtcac tcgggttgct cgtgtgctcc gcccaacgca aacagtccag
      301 cgcgaagatg cgcatttcgt gcgtggtgac catcgctatc cgaccgtcgg ccgatcaatt
      361 cccgccagtt cgttaagcgc tagttcggaa aggcgttcgg gatcgcgctc gccttggtca
      421 acgtgccgga gaataatcag cgccagctgg cgtcgcacgc gcgggtcgcc gttgatcttg
      481 ggggatacgg atcgacagac attgtcaaat gctgccgaca tcgccactac gatttcaggg
      541 ggatagacac ggtcatggtc aagtgtaacc aggtccagca caacactcgc ccttcaccct
      601 tggacatgca acccaaggaa gctaacaagc gagactgcgg tcatctgttc aaaactgctc
      661 acgcgagcaa attatcgctg ttcaaaattg atcaccgtga accttgaatg tgcggcggct
      721 tctcatgcaa gaccacatcc aacagcgagg agtggacttt caggtcgccg ttatactcga
      781 tcagccccaa ctcccgaaac ctgttcatga agaagctcac gcgcgaacgc gtcgtaccga
      841 tcatctccgc aagtgtagct tgatcaacgc ccggaacgat tggctccggc ttcccttcct
      901 ggccgacctt cgacaggatg aggagcgcac gcgccaatcg cttctcgctg ctattgaaga
      961 gctgatcgac caaatcctct tcaactcgaa tagtccgggc caagaggtac gacgtgaaca
     1021 tctcggagaa tttcggctct tcgcgaagcg tcttcaacat cgtcgtcttg tctatccggg
     1081 cgatcacgct ttccgtcatc gcggcggcgg tgg
//
LOCUS       DASTWE010000255         1460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1008837, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000255 DASTWE010000000
VERSION     DASTWE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008837"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFT30_02775"
     CDS             complement(<1..287)
                     /locus_tag="VFT30_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5091576.1"
                     /translation="MSLLMTEIHCRIDWENIPNEAMMAKPTMNGRKSYVPLDNRYAER
                     VAITCRVRYVGEVPTQPHQGEGLTKNISVSGCRIISDRPVTRGTLLTLTIAL"
     gene            complement(354..>1460)
                     /locus_tag="VFT30_02780"
     CDS             complement(354..>1460)
                     /locus_tag="VFT30_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU5091577.1"
                     /translation="KFDELKAVYQLWSGRVPLVVVPTIFDQTTAAEMEEAGAKIIIYA
                     NQPVRAAIRAMTDALDLIKKDTRPGAANDRIVTLPEVYDIVGVPQMEQDEKQFLPVGS
                     ERITAIIAAAGFEKQLLPLIEDKPKCLLDIKGKTILDRQISALNECDIKEIALVRGYK
                     KEAIALPNIRYYDNDRYEETGELVSLFCAENELKGRTIVLYGDIIFDTSILEKLLKSP
                     ADIALVVDLAWFDQEQRNAQPTHLNPDLVSLAVPPGKSYLSRFVMPEGEHQVVKIGQQ
                     LPHELAHGEFIGMAMFSEKGTQALCECYHRVHKQHQSSPFHESASLAKASFTDMLQEL
                     IDQGYRIQAVPIFKGWMEVDSFEEYQKAWAKIRQ"
BASE COUNT          316 a          375 c          392 g          377 t
ORIGIN      
        1 agtgcaatgg taagggtcaa tagtgttcca cgcgtcaccg gccggtcgct gatgatgcgg
       61 caaccagaaa cagatatgtt tttcgtgagt ccttcgcctt ggtgaggctg cgtcggaact
      121 tcacccacat agcgcacgcg acacgtgatg gcaacccgtt cggcataacg gttatctaaa
      181 ggcacatagg attttcggcc attcatcgtc ggtttcgcca tcatagcttc gtttggaata
      241 ttctcccaat caattcggca atgtatctcg gtcatgagga gtgacatggc acaccgccta
      301 taaaattggg aatattcctg gcgacgagga gagcagctac cgtagatggg gaactactgt
      361 cgaatctttg cccaggcttt ttggtactct tcgaaggaat caacttccat ccagccctta
      421 aaaatcggta cggcttggat gcgatagccc tggtcgatca gttcttgcag catgtcggtg
      481 aaagatgcct tggcaaggct tgcagactcg tggaaggggg aggactgatg ctgtttgtgg
      541 accctgtggt agcactcgca aagggcctgg gtgcccttct ctgagaacat ggccatgccg
      601 atgaattcgc catgcgccag ttcgtgcggt agttgctggc cgatcttcac cacctggtgc
      661 tcaccttccg gcatcacaaa tcgggacaaa tagcttttgc cgggtggaac tgccagtgag
      721 acaagatccg gattaagatg ggtcggctgg gcatttcgtt gttcctgatc aaaccaggcc
      781 aaatcgacaa ccagagcaat gtccgcggga ctcttgagta acttttctag tatcgaggta
      841 tcaaagatga tatcgccgta cagaacgatg gtccgccctt ttaactcatt ttcggcacag
      901 aagagtgaaa cgagctcgcc tgtttcctca taccgatcat tatcgtaata gcggatattg
      961 gggagcgcga tcgcttcttt cttatacccg cgaaccaaag caatttcttt gatgtcgcat
     1021 tcgttgagtg cgctgatctg gcgatcgagg atcgttttcc ctttaatgtc gagcaaacac
     1081 ttcggcttat cctcgatcaa cggcaggagc tgtttttcga atccggcggc ggcgataatc
     1141 gcggtgattc gttcgcttcc caccggaagg aactgttttt cgtcttgttc catttgcggg
     1201 acaccgacaa tgtcgtacac ttcgggtagc gtgacgattc ggtcgttcgc agccccgggg
     1261 cgcgtgtcct ttttgatgag atccagcgcg tccgtcattg cacgaatggc cgcccgtacc
     1321 ggttggttgg catagatgat gatctttgcc ccggcttctt ccatttccgc cgccgtggtc
     1381 tgatcaaaaa tggttgggac cacgaccaga gggacgcgcc cggaccacaa ttggtatacg
     1441 gccttcagct cgtcaaactt
//
LOCUS       DASTWE010000256         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1428837, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000256 DASTWE010000000
VERSION     DASTWE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428837"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1097..1900)
                     /gene="dapB"
                     /locus_tag="VFT30_02785"
     CDS             complement(1097..1900)
                     /gene="dapB"
                     /locus_tag="VFT30_02785"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU5091578.1"
                     /translation="MIKVIVAGAAGRMGCRLVSLIKDSTALTLAGALEGKGHPSLGED
                     AGEIAGSGRADLLITEDLSVLMERGEVVIDFSAPAATLEHMRTVAHHRRAMVIGTTGF
                     SALQLDELRSLTQQIPCVFSPNMSVGINLICKVIGEMARTLGDDYDIEVIEAHHRQKK
                     DAPSGTALKIAEVLAHSVNRDLNQVGVYARKGLIGERTKGEIGIQTIRAGDIVGDHTI
                     LFGGMGERIEVTHRASSRDTFARGALRAARWVVRQPAGLYDMMDVLSLR"
     gene            complement(1927..>2755)
                     /gene="dapA"
                     /locus_tag="VFT30_02790"
     CDS             complement(1927..>2755)
                     /gene="dapA"
                     /locus_tag="VFT30_02790"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU5091579.1"
                     /translation="GKVEERALAELIEWHIANGTNGIVPCGTTGESATLSHTEHNRVI
                     ELTVEVVRRRVPVIAGTGSNSTEEAIALTKHAKHAGADGALLITPYYNKPTQEGLYRH
                     YKVIAEAVDLPLVLYNIPGRTGVNMLPSTIARLSGVNTIVGVKEGSGSVQQASDIVQM
                     CGDRLTVLAGDDSLTLPMMAVGGKGVITVTANIMPMEMAGLVKAFADGRIDEARRIHF
                     KLSPLFAALFYETNPIPVKEALGLMRKIDPELRLPLCSMGQDTREKLIRVLKDAALI"
BASE COUNT          636 a          752 c          687 g          680 t
ORIGIN      
        1 caatgtcaga ttaccctcat gtttgacaaa gacgagaaac tttctgacta tacctacaca
       61 ggctgctaat caacttgttg caagcctgcc ggattccttc tccagcaatg atttcatgta
      121 gttcctgaat gccctcctag acggggtgct cacttcggaa cttcaccgaa cactcctggc
      181 tgcagaaaca gtactctcgt cccccaattg tttcgatcac ggcgattctc ttcgggacga
      241 atatgtgaca caccggatcc tcgatcatct ggtcttgttc ttcctcttgg gcttgcgcgg
      301 tcgatttctc ccggccattc cgaatcggtg ttttgaatcc acgaaatatt tgtctaagca
      361 agaagtagag aactgtgaga agtaaaacga ctaggattaa tctatacata gtggtctatc
      421 acagggacac cacaatccta tgaaagccgt gggagcctgt caagataacc acagcgtttg
      481 attgtaagaa tactactggg gaagacatgc acaaacgacc tatgttcata ggtccgattg
      541 ggactcttgt cagtgccgtc cagtacttca ggaggattgg cttgccctgt cctatggtgc
      601 taaagtgccc ctatgaaaac ctgcgataaa tgccatgggg cgatgctacc agaacgagcg
      661 gtggatttgg acgctggact ggcgatcact gtatttgcct gtttgaattg tggtcgtcgg
      721 aaagcagcgg atcaggaacc tcggccgatt acggctcgac attaatccta aatcctaccg
      781 tcgaacccca ggttaccacc ttctgatgag cggggctacg aggtgcttgt gaggcacctt
      841 ccacgccaat gtgggtaatg gtgctcgtcc tttcggagac gtcactactt acgaacgaac
      901 attgaccgtc atgcggatta cgacacatct aacagaaatc accgaaagca ttgaatgcct
      961 gagggtcaac tctacctttt tacttaaccg ccagtcctga attcgacggt tgtctttttt
     1021 cgatcagatg cacttggtgg gatattcgct gaaaacacaa tcggctgctt atgatgatgt
     1081 gggcatgaaa gaagagtcaa cgaagactta agacatccat catatcgtac aaaccggccg
     1141 gttggcggac aacccatctc gcggcacgca gggctccacg ggcgaacgta tcgcggctgc
     1201 tggctcggtg ggtgacctcg attcgttctc ccattccgcc gaacagaatc gtatgatcgc
     1261 cgacgatatc gcctgcgcga atggtttgta taccgatctc tccttttgtt cgctcaccga
     1321 tcaacccctt gcgggcataa actccgactt ggttcaaatc tcgattaacc gaatgggcga
     1381 ggacttcggc aatcttgagg gctgtgccgc ttggcgcgtc cttcttctgc ctatggtgag
     1441 cttcaatgac ctcaatgtcg taatcgtccc caagagttct ggccatctcg ccgatgactt
     1501 tgcagatcag gtttattccg acactcatgt tcggagaaaa gacgcaggga atctgctggg
     1561 tcagtgacct cagttcatcg agttgaaggg ccgaaaaacc ggtagtcccg attaccatgg
     1621 ctctccgatg gtgagcaacc gttcgcatat gctcgagtgt ggctgcgggc gcggaaaaat
     1681 cgatcaccac ttctcctcgc tccatcagaa cggagagatc ttcggtgatg agaagatcgg
     1741 cacggcccga tccagcaatt tcgcccgcat cttctcccaa cgacggatgc cctttcccct
     1801 ctaaggcccc tgccagggtc aaagccgtag aatctttgat cagtgacacc agccgacagc
     1861 ccattcggcc ggctgcaccc gctacaatga ccttgatcat cttgtcgtgc acctagatat
     1921 tactagttag atcaacgccg catctttgag gacgcgaatc aatttctcgc gtgtgtcctg
     1981 gcccattgag cagagcggta atcgcagttc cgggtcgatc ttcctcatga gtcccaaagc
     2041 ttctttgacc ggaatggggt tagtctcata gaacaatgcc gcaaagaggg gagagagttt
     2101 aaaatgaatc cgtcgtgctt cgtcgattct cccatcagca aaggctttca caagcccagc
     2161 catttccatc ggcatgatat tagccgttac ggtaatgacg cctttcccac cgaccgccat
     2221 catcggtaac gtaagtgagt cgtcgccagc cagcacggtt aggcgatcac cgcacatctg
     2281 cacgatatct gaggcctgtt ggacggagcc actcccttcc ttgaccccga caattgtatt
     2341 aacgcctgag aggcgggcga tcgttgacgg tagcatgttg actccagtgc gaccggggat
     2401 gttgtacagg actaaaggca ggtcgacggc ctccgcaatc accttatagt gtcgatagag
     2461 tccctcttgg gtcggtttgt tgtagtaagg ggtaatgagc aacgcgccgt cagccccagc
     2521 gtgctttgcg tgtttcgtga gcgcaatggc ttcttccgtg ctgttcgaac cggttcctgc
     2581 aataaccggt acccgccggc ggaccacctc gaccgtcaac tcaatcaccc gattatgctc
     2641 ggtatgagaa agcgtcgctg attcacccgt ggttccgcag gggacaatgc cgttagtacc
     2701 attggcaata tgccattcga tcaactcggc caaagcacgt tcttcgactt tgccc
//
LOCUS       DASTWE010000257         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1461102, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000257 DASTWE010000000
VERSION     DASTWE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461102"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..807)
                     /locus_tag="VFT30_02795"
     CDS             complement(247..807)
                     /locus_tag="VFT30_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091580.1"
                     /translation="MSNYSIALFLHIVGAMGFFVVQGVEWIGLSQIRRARLPEEARAI
                     LGTVKSADWLGFISMPTTIVTGIYMMLTVWGWVPWILVVLGALILEIVLFVVLAKPRM
                     VAMEQALATEKGFVSQTFHDLVNNPILWISVRTRVAIILGIILLKFTKPNLEGSLLTI
                     GIAVMVGLASALSMSHRVRVQEGRVD"
     gene            complement(903..977)
                     /locus_tag="VFT30_02800"
     CDS             complement(903..977)
                     /locus_tag="VFT30_02800"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU5091581.1"
                     /translation="MRARYARGEITSEQYGQMRRDLES"
BASE COUNT          228 a          271 c          260 g          244 t
ORIGIN      
        1 aggatgttaa tcgtatcggt aatgtttgga agtacgttca tatttatctt ctccatgttg
       61 ttccggcaag atgaatgcta tatgtccgtt ctacagacag ggtgtattca tcaaacgatt
      121 ctcggcctag ttcggggttg aaagcccgca tcgaggaaag catcgagggg atagcgtagc
      181 tgtgaactgc actatcccct cgttactgcc aggcagatgc agagcagtgc atgcaactgc
      241 agcacgtcag tcaacccgtc cctcttgtac tcgcacacgg tgggacattg acagggctga
      301 ggcaaggccg accatgaccg ctataccgat ggtgagcagc gacccctcca ggttgggctt
      361 ggtgaatttg agaaggatga tccccaggat gatcgctact cgggtccgga cggagatcca
      421 caggatcggg ttgttcacca gatcgtggaa ggtttgggaa acgaatcctt tttccgtggc
      481 cagagcctgc tccatagcca ccatccgtgg cttggcgagc accacaaaga gcacgatttc
      541 cagtatgagt gctcccagca ctaccaggat ccacggcacc caaccccaca cggtcagcat
      601 catatagatc ccggtaacga tcgtggtcgg catcgagata aacccgagcc agtctgcact
      661 tttgactgtt cccaggattg cacgagcctc ttcgggcagt ctggcgcgtc ggatttgcga
      721 gagtccgatc cactccactc cctgcaccac gaaaaacccc atcgcgccga cgatgtgtaa
      781 gaacaaggcg attgaatagt tgctcatgat ttactccttt ctatattcct tcgatttacc
      841 atttgcagtt cggttggcaa ctgtttgcct gatatatcca gccgcttgct ggagaagtta
      901 gatcaactct ctaaatcgcg acgcatctgc ccgtactgtt cggaggtgat ttctccacgc
      961 gcatagcgtg ctcgcaagat ctcgaccgga gtttggctgg ttt
//
LOCUS       DASTWE010000258         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_944106, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000258 DASTWE010000000
VERSION     DASTWE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944106"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFT30_02805"
     CDS             complement(<1..265)
                     /locus_tag="VFT30_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEU5091582.1"
                     /translation="MSKPLDVLFVTPPSRVQVYQDLSRDLAAIEPPVWAGLLGTFLRR
                     RGYEAAILDAEASGFDQQQTAEAIATADPRLAVFVIYGQQPSAS"
     gene            complement(249..>1527)
                     /locus_tag="VFT30_02810"
     CDS             complement(249..>1527)
                     /locus_tag="VFT30_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5091583.1"
                     /translation="PWVPSILYEEVVRPTGRPHVREDIMVTTERTIMLPFSTRAERLI
                     GQEMFKVMDRAQALERDGQTIYHLELGNPRVAPPCEIIDATLEAIRAKQLGYAPMAGI
                     RELRDAVGDRIVRKTDRRINGDSVVISPANLLISQFLDLTCNPGDRVVLFTPAFPSYW
                     AAAAHSGLTVTAVPLSQENGFHLDESQVEAAVAARPKAIIVNSANNPTGAVYTRSILR
                     LLARRCEEECIWLLSDETYADLAFGRPFFSLTSCESSQLVVMSSFSKVFSIPGYRIGY
                     ALAHPAVVSKFALSTSTLISCLPIFTQLGCVAGLSVLDRYAERVRTQCRRVTSRCTEW
                     INRSSIIRCTTPESGFYLFIDIRSTGLDDVTFCKRLLEERHTAVTPGRSFGSSCSSYI
                     RIATCGQESDVLEGVRRVVEYAHELGGANVKAA"
BASE COUNT          366 a          446 c          431 g          284 t
ORIGIN      
        1 tggaagcaga cggctgttgc ccgtaaatga caaagaccgc cagtctggga tcggccgtcg
       61 cgatcgcctc cgcagtctgc tgctgatcga accccgacgc ttccgcatca agaatggccg
      121 cctcatatcc gcgacgacga agaaatgtgc cgaggagtcc cgcccacaca ggcggttcga
      181 tggccgccag atcccggctg agatcctggt acacctgcac ccggcttggc ggcgtgacga
      241 acagcacgtc aagcggcttt gacattggct cctcctaatt catgtgcata ctcgacgacc
      301 cgccgcacac cttccaaaac atcggattcc tggccacaag tggcaatgcg aatatatgac
      361 gagcaagatg agccgaaact gcgaccaggg gtcacggcag tgtgccgttc ttcgagaaga
      421 cgcttacaga atgtgacatc atcgagaccc gtagagcgga tatcgatgaa caggtaaaag
      481 cccgattccg gcgttgtaca acggatgatg ctcgaacgat tgatccactc ggtacaacga
      541 ctcgtgacgc ggcggcattg cgttctgacg cgctcggcgt accgatccaa aaccgacaac
      601 ccggcgacgc atcccaattg agtgaaaatg ggcagacacg agatgagcgt ggacgtcgag
      661 agcgcaaatt tgctcacgac agccggatgc gccagggcgt aaccgatgcg atatcccggg
      721 atggaaaaca ccttcgagaa ggaggacatg accacgagtt gcgaagattc gcatgatgtg
      781 agactgaaaa agggccgccc aaacgcaaga tctgcatagg tctcatcact caacagccaa
      841 atacattcct cttcgcaacg tcgggccagc aacctcagta tggatcgggt atagaccgca
      901 ccggttggat tgttcgcgga gttcacaatg atcgccttcg ggcgcgcagc cacggcagcc
      961 tcgacctgcg attcgtccaa atgaaatccg ttctcttggg acaaaggcac cgccgtcacc
     1021 gtgagaccac tatgggcagc cgcagcccag taagacggaa acgcaggggt gaatagcaca
     1081 acccgatccc cgggattgca ggtgaggtcg aggaattgac tgatgaggag attggcgggg
     1141 ctgattacca cggaatcgcc gttgatccga cggtctgttt ttcgcacgat tcgatccccg
     1201 accgcatccc gcagctctcg aatccccgcc ataggcgcgt atccaagctg cttggcacga
     1261 atggcctcca aggtcgcatc gatgatctca caaggcggcg cgacccgagg attgccgagt
     1321 tcgagatggt agatcgtctg accatcccgt tcgagcgctt gagcacggtc catcactttg
     1381 aacatttcct ggccgatgag gcgttcggca cgggttgaaa atggaagcat gatcgtccgt
     1441 tctgtcgtta ccatgatgtc ctcacgcaca tgcggccgac ctgttggacg cacgacctcc
     1501 tcgtagagaa tcgacggaac ccacggt
//
LOCUS       DASTWE010000259         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1202608, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000259 DASTWE010000000
VERSION     DASTWE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202608"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFT30_02815"
     CDS             <1..469
                     /locus_tag="VFT30_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091584.1"
                     /translation="QTNHPDIYAAGDVAETKDFLSDDRSIYAIWPTAIDQGRAAGVNM
                     TGKKQVYPGSLGMNVVELFNVTLAQLGRFREGPGDSVKMLSPKGDEGSYRKIVVDKGG
                     TLVGAVYVGNETGVAEMGVIHSAIKRREHWQGFQEGRPPQFSYGAVMHNVPRY"
     gene            472..720
                     /locus_tag="VFT30_02820"
     CDS             472..720
                     /locus_tag="VFT30_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5091585.1"
                     /translation="MADTVTVAVKLFATLKKYLPPGSEEGTSVTLPAGSTVLDVIDAL
                     KIPPEHATMLVAGDTYIEKTTPVTDGLQLSIFPPLAGG"
BASE COUNT          254 a          280 c          300 g          249 t
ORIGIN      
        1 gcaaaccaat catcctgaca tttacgccgc tggtgatgtc gccgagacca aagatttctt
       61 gtccgacgac cgctcgatct acgcgatttg gccgaccgcc attgaccaag gccgggcggc
      121 tggcgtgaac atgacaggga agaagcaggt atatcctggc agtctgggga tgaacgttgt
      181 cgagttattc aacgtgaccc tggcacagct cgggcgtttc cgtgaaggtc ccggggatag
      241 tgtaaaaatg ctctcaccta aaggagacga aggatcgtat cggaagattg tcgtggataa
      301 gggtggtacg ctagtcggcg cggtctatgt cggcaatgag accggcgttg ccgagatggg
      361 tgtcattcat agcgccatca aacgacgaga gcattggcag ggtttccaag aaggtcgtcc
      421 gccacagttc tcgtacggcg cagtcatgca caacgttcct cgttattagt tatggcagat
      481 acggttaccg ttgccgtcaa actgtttgcg accctcaaga agtaccttcc gcctggcagt
      541 gaggaaggta cttctgtgac tctccccgca ggctcgactg tcctggacgt tattgatgcg
      601 ctcaaaattc cccccgagca tgcgacgatg ctggtagcgg gagatacgta cattgagaaa
      661 accactcccg tgacagacgg tcttcagctt agtatcttcc caccgctagc cgggggatga
      721 gacgctgagc cggtttcatc gatcgagact gtaagctgac gactcgactg tcgatcattc
      781 cagtgtcgtg gcccggatga agtccgggaa gaactctcct taccgcgaat tgtcccaatc
      841 ctcacgtagc gagttgtaga ctcaaaccgt cggtggccca ctgctgccgc aggttctcct
      901 tgtgtggacg cgacaaactg cgtataaact catcgcggcg ttgcttgagc aggcatcata
      961 ccaacaaata ggaaaactct atcttctttt tagcgggagt ccatagtgaa aaaacagata
     1021 gttgttactg ttaatggtga acaacacacc gacgaagttg agccacggct cttgctggtg
     1081 cat
//
LOCUS       DASTWE010000260         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_847327, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000260 DASTWE010000000
VERSION     DASTWE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847327"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFT30_02825"
     CDS             complement(<1..355)
                     /locus_tag="VFT30_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5091586.1"
                     /translation="MRDIGPYSNYRLTVRLELANKPGIFARVAALLAEEQANLGAVDL
                     VAATKTRMVRDITFDVLSEEHGEKVLSRLDELPDVIVLSASDPIFLLHLGGKIRVGSK
                     FPISTRNVLSMVYTPG"
     gene            494..>1515
                     /locus_tag="VFT30_02830"
     CDS             494..>1515
                     /locus_tag="VFT30_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5091587.1"
                     /translation="MSYAPEKRTPNRLIHETSPYLLQHAYNPVEWYPWGPEALQLAKE
                     KNRPILLSIGYSACHWCHVMERESFENEAIADLMNRWFICIKVDREERPDLDEIYMAA
                     TVTMNHGQGGWPMTVFLTPAQEPFFAGTYFPPEDRWGRPGFGSVLKKIADYWDTRPSE
                     VQDQAKELTSQLQGLRQLPSPVSISEAVLEEAVDQFKEDFDETHGGFGTAPKFPPAMG
                     LSFLLRSHRRSGNPHTLTMVTKTLDMMAAGGIYDHIGGGFARYSTDARWLVPHFEKML
                     YDNALLARVYVEAYQVTQKPLYRQVATEVLDYVRREMTGPEGGFYSSTDADSEGVEGK
                     FFVWTPT"
BASE COUNT          356 a          431 c          396 g          332 t
ORIGIN      
        1 ctcccggcgt atacaccatt gagaggacat ttctggtgct gatcggaaac ttgctcccca
       61 ctcgaatctt gcctccaaga tgaaggagaa atatggggtc ggatgccgag agcacgatga
      121 cgtccggcaa ctcgtccaga cgagaaagaa ctttttcacc gtgctcctcg ctcagaacgt
      181 cgaaggtgat gtcacgaacc atgcgcgtct ttgtcgccgc cacgagatcg acggcaccga
      241 gattggcctg ttcttctgct aataacgccg ccactctggc aaaaatgcct ggtttattcg
      301 cgagttccag ccggacagtc aaacgataat tggaatatgg accgatgtct ctcattcgtt
      361 tccttcctcg tctagactac cacaacttac aaccacggcc agaagaacaa accgaataga
      421 gtcccgctac agattacgcg ccttcccctc gactccgttg acggtgttct catcacggtg
      481 ctaggttgac cccatgtcgt acgctcctga gaaacgcaca cccaatcgac tcattcatga
      541 aacgagtccc tacctgctgc aacatgccta caaccccgta gagtggtacc cctgggggcc
      601 ggaagccttg cagcttgcca aagaaaaaaa tcgtcccatc ttgctctcaa tcggctattc
      661 cgcctgccac tggtgccatg tcatggaacg ggagtctttt gaaaacgagg cgattgcgga
      721 cctcatgaat cggtggttta tctgcatcaa agtagaccgg gaagaacgac ccgatctcga
      781 tgagatctac atggccgcca cggttacgat gaatcatggt cagggtggtt ggccgatgac
      841 ggtgtttctg acacccgctc aagagccgtt ttttgcaggc acgtattttc ctccagaaga
      901 tcgatgggga aggccaggct tcggcagcgt cctgaagaag attgccgact attgggacac
      961 acgtccctcg gaagtccagg atcaggctaa agagctgacg tcccagttac aaggcttgag
     1021 acaactcccc tcccccgttt ccatcagcga agcggtgctg gaagaagcgg tcgaccagtt
     1081 caaggaggat ttcgacgaaa cccatggtgg gttcggaaca gcgcccaagt ttccgcccgc
     1141 catgggattg tcgttcctac ttcggagcca ccgccgctcg ggaaatcccc atacgctcac
     1201 gatggtgacc aagaccctcg acatgatggc tgctggagga atctacgacc acatcggcgg
     1261 tggctttgcc cgctattcga ccgatgcgcg atggctggtg ccgcattttg aaaaaatgct
     1321 gtatgacaat gcgctcctgg cacgcgtata tgtcgaggcc tatcaagtca cccagaagcc
     1381 tctctatcga caggtggcca ccgaagtgct cgactatgtg cgccgggaaa tgaccggacc
     1441 ggaaggcgga ttctattcat cgacggatgc cgactctgaa ggtgtcgaag gaaagttctt
     1501 tgtttggact ccgac
//
LOCUS       DASTWE010000261         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_912023, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000261 DASTWE010000000
VERSION     DASTWE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912023"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFT30_02835"
     CDS             complement(<1..979)
                     /locus_tag="VFT30_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091588.1"
                     /translation="MAVSPTGKRVVVEARGEVFTIPADKGDVRNLTNSSASAERDPAW
                     SPDGKFVSYFSDKSGEYKLVIESQDGLTPPREIQLERPTHYYTASWSPDSKKLLYTDT
                     NLKVWVIDVATGQAKVVGNDPWMVPTRTLNPVWSSDSKWVAYSSRLRSLYHAIFISNV
                     ETGESKQVTDGLADAVWPAWDSNGKYLWFLASTDFGLRSQWLDMTSYDREENFGLYFA
                     VLKKTDPSPLLPESDEDRGVGTGTRRSVPGACPGDAGGGGEPAASGDTGGARGQRGPV
                     TVQIDLDGLQQRIVSVPGVPERQYSDLQAGVDGSVFYLEAPRGGGAGGGG"
BASE COUNT          224 a          308 c          280 g          238 t
ORIGIN      
        1 taccgccgcc acccgcgcct ccaccgcgcg gggcttcaag gtaaaagacc gagccgtcca
       61 ccccagcttg caaatctgaa tactgtcgtt caggaactcc tggtacagac acaatgcgct
      121 gctgaagacc gtcaagatct atctgcacgg tcactggacc acgttgccct ctcgctcctc
      181 cggtgtcacc cgacgctgct ggctctccac cgccaccagc atcgcctgga cacgcgcctg
      241 gaaccgagcg tctagtgccc gttccgacac ctcgatcttc gtcactctcg ggaagtaatg
      301 ggcttgggtc cgtcttcttc aaaacggcaa agtagagtcc gaaattttct tcgcgatcgt
      361 aggaggtcat atccagccac tgtgaacgca gaccgaaatc tgtagacgcg agaaaccata
      421 gatactttcc atttgaatcc catgcgggcc agactgcatc ggcgagtccg tcggtcacct
      481 gtttactctc gccagtttcg acattgctaa tgaagattgc gtgatagagc gagcgcaatc
      541 gacttgagta ggcgacccac tttgagtcgg aactccaaac cgggttgagc gtgcgagtgg
      601 gtaccatcca cggatcgtta ccgacgactt tggcctgtcc agttgcgacg tcgatgaccc
      661 aaactttcag gttcgtatcg gtgtaaagaa gtttctttga atctggtgac cacgaagccg
      721 tgtagtaatg cgtagggcgt tcgagttgaa tctcgcgagg cggagttaga ccatcctgcg
      781 actcgatcac caatttgtat tcgccagact tgtcgctgaa atacgaaaca aacttgccat
      841 cgggagacca tgcaggatct cgttcggcgg aggcgctgga gttggtcagg ttgcggacat
      901 cacccttgtc cgctggaatc gtgaacactt cgccccgtgc ctcgaccacg acgcgcttgc
      961 cggttggtga cacggccatg ttcgtaattc gattcgacac gtcctcccag ttgggcatca
     1021 tccaggggaa gtcgcctgtg gccgtgatgt
//
LOCUS       DASTWE010000262         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_912050, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000262 DASTWE010000000
VERSION     DASTWE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912050"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..545)
                     /locus_tag="VFT30_02840"
     CDS             complement(156..545)
                     /locus_tag="VFT30_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091589.1"
                     /translation="MKPTGMCPTACYVVLGILTACTTNGPAPPTASSPTPVVTQPTSQ
                     IRASDRMFYWREEARELHAMATHRDREAELMLRKKPGSTTHEFVKQMQQLAHQLHQAA
                     TYADAQAKEAEREIPPDMLQQFHSALR"
     gene            complement(542..769)
                     /locus_tag="VFT30_02845"
     CDS             complement(542..769)
                     /locus_tag="VFT30_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091590.1"
                     /translation="MSRTILICSGRSRYRDGAMELIHIGQAVPDVDGRLDYFLRTAFN
                     HPTWAECYKVAALDAHNELAMTASCLRKEAT"
     gene            complement(882..>1289)
                     /locus_tag="VFT30_02850"
     CDS             complement(882..>1289)
                     /locus_tag="VFT30_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091591.1"
                     /translation="RVLIVEHDEGERYVLSLLLENEGYNVHMVSEEGPALEEMKRRRF
                     DVVISAHHMPQINGFRLVLLMRLLWPDTPMILLLGDDTSLPETAEQGEAYGNLHKPYN
                     FGELLELMKDAIQFTREHRSQTSQSIMSRRQSP"
BASE COUNT          273 a          366 c          337 g          313 t
ORIGIN      
        1 gaccgctacg gcctcggcaa acctcggctg gccaccggcg tcgatggttc ggctccaacc
       61 ggttcctgaa agtggagcaa acccctaaaa cggcgagaga gtcctcctca aaaccgtaaa
      121 acggtgtcgc tcccgttgag actgtcttca gtgaatcatc gcagcgccga gtgaaattgc
      181 tgaagcatgt caggcggaat ttcacgttcg gcttcctttg cctgtgcgtc tgcataggtg
      241 gctgcttgat gcagttgatg cgcgagctgc tgcatctgct ttacgaattc gtgcgtcgtc
      301 gaccctggtt ttttcctcaa catcaactcc gcctctcgat ctctatgggt tgccatagca
      361 tggagctctc gcgcttcctc gcgccagtag aacatgcggt ctgatgcacg tatctgtgat
      421 gtcggctgcg tcacgaccgg cgtcggggag gacgctgttg gaggtgccgg accgttcgtc
      481 gtgcaggcgg tgagtatccc gagcaccaca tagcaagcgg tgggacacat tcccgttggt
      541 ttcatgtggc ctccttccgc aagcaggacg ccgtcatagc aagttcgttg tgtgcatcca
      601 gcgcggccac cttgtaacat tcagcccacg ttgggtggtt gaaggccgtg cgcaagaaat
      661 aatccaacct gccgtccaca tccggcaccg cctgaccgat atggatcaat tccattgccc
      721 cgtcccgata gcgtgaacgg ccagagcaaa tcaaaatcgt acggctcacg atccatggct
      781 agtcagtggt ccaggatcat tggatcgaga tagtaagggc gtctctccca gatcgtttga
      841 gcgggatccg cttaagacgt tcattaattg cgccgtgttc ctcaaggcga ctgacgtcgc
      901 gacatgatag attgcgatgt ttgtgatcga tgttcacggg tgaattgaat ggcgtctttc
      961 ataagctcca gtagttcacc gaaattgtaa ggcttatgaa ggttgccata cgcttctcct
     1021 tgctcggccg tttccggcaa actcgtatcg tcccccaata agagaatcat cggtgtgtcc
     1081 ggccagagaa gccgcatcag caagacaaga cgaaatccat tgatttgggg catgtgatga
     1141 gcactaatca ccacatcgaa ccgtcgacgt ttcatttctt ccaatgctgg gccttcctcc
     1201 gacaccatgt gaacgttata cccttcattt tccagcagta gactcaacac gtaccgctcg
     1261 ccttcgtcat gctccacgat cagcactct
//
LOCUS       DASTWE010000263         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1429081, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000263 DASTWE010000000
VERSION     DASTWE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429081"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VFT30_02855"
     CDS             <1..1193
                     /locus_tag="VFT30_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5091592.1"
                     /translation="EAETHMSSIDLFNPTPEHSLLRQTVRDFVREQVEPQALAHDRDE
                     RFNLPLFRQLGELGLLGITVASEHGGAGMDAVAAVIVHEELSASDPGFCLAYLAHSML
                     FVNNFYHNASEEQLHRYLGKVISGEWVGGMCMTEPSVGTDVLGMKTQAVRRGDSYYVN
                     GRKMFITNGAIDDKTLGNIFLVYARTENGISSFLVEKGFPGFSLGQRLHGKAGMRASM
                     TAELVFEDCVVPVGNRLGEEGESTIHMMRNLEIERVTIAAMSTGIAARCVEVMTKYAS
                     ERTAYGQPINQFGQIQRYIADSYAQYMAARTYLYHTAARMSLDKPGQRIDSDGVKLVA
                     TTMAKVVADNAMQVLGGYGYFNEYIVERLWRDAKLLEIGGGTLEAHQKNITKDLTRRM
                     DLIR"
     gene            1203..1409
                     /locus_tag="VFT30_02860"
     CDS             1203..1409
                     /locus_tag="VFT30_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091593.1"
                     /translation="MNRGNWNRFMIVLMIAVIVFGLQEIFRSPEPMNETRYLLSIGIA
                     ILVIFLKVGEIKKAESETQKPSQV"
BASE COUNT          377 a          368 c          466 g          356 t
ORIGIN      
        1 cagaggcgga gacgcacatg tcaagtattg acctttttaa tcccactccc gaacattcgt
       61 tgctccgaca aacagtgcgg gactttgtcc gcgagcaagt tgaaccccag gccttagctc
      121 atgatcgtga cgagcgtttc aacttgccgc tgtttcgcca actcggggag ctagggttac
      181 tcgggattac cgtggcgagc gagcacggcg gagcggggat ggacgccgtg gcggcggtca
      241 tcgtgcacga agagctgtcg gcttcagatc cgggattttg cctggcatac ctggctcact
      301 cgatgttatt tgtcaacaac ttttatcata atgcgagcga ggaacagctt caccgctatc
      361 tcggcaaggt gatctccggc gaatgggttg gtgggatgtg catgactgag ccgtcggtag
      421 ggaccgacgt gttaggaatg aagacgcaag ccgtacgccg aggcgattcc tattatgtga
      481 atggacggaa aatgttcatc acgaacggcg cgattgacga caaaacgctt ggtaatatct
      541 ttcttgttta tgcgcgtacg gagaatggga ttagttcctt cctggtcgag aaagggttcc
      601 cggggttttc gctcgggcaa cgactgcacg gcaaagcggg aatgcgtgcg tctatgaccg
      661 cggagctggt gtttgaggat tgtgtcgtac cggtgggaaa tcgtcttggg gaggaaggcg
      721 agagcacgat tcacatgatg cgcaatctgg agattgagcg ggtcacgatt gccgcgatgt
      781 ccaccggtat agctgcacgg tgtgttgagg tcatgaccaa gtacgcgagt gaacgaacgg
      841 cgtacgggca gccgattaac caattcggcc agatccaacg ctacattgcc gattcgtatg
      901 cgcagtacat ggccgcccgt acgtatctgt accatactgc cgcgaggatg agtctcgaca
      961 aaccggggca gcgtattgat tctgacggag tgaagttagt ggccaccaca atggccaagg
     1021 ttgtggcgga taatgccatg caggtattag gcggctatgg ctatttcaat gagtatattg
     1081 tcgaacggct gtggcgtgac gcgaagcttt tagagattgg cggaggcacg ttagaagcgc
     1141 atcagaaaaa catcactaaa gatctgacgc ggcgaatgga tttgattcgc tagggagttc
     1201 ctatgaatag aggcaactgg aatcgattca tgattgtctt gatgatagcg gtgatcgtct
     1261 tcggtctcca ggaaattttc cgctcgccag agccgatgaa tgaaacacgc tatctgctga
     1321 gtattgggat agcgatcctg gtgattttct tgaaagttgg agaaattaag aaggctgagt
     1381 ctgaaacaca gaaaccgtcg caagtgtgaa ggaactcggc agacacagcc tgccctacaa
     1441 cgctactcgg aaacaaaatg cccaacccga acgatcctcc tctggattcc agcccttcga
     1501 ctaggctcag gacaaacttt cgttggcatg acaaagacga cacgctccgc attccgggcg
     1561 gacagcg
//
LOCUS       DASTWE010000264         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_976747, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000264 DASTWE010000000
VERSION     DASTWE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976747"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VFT30_02865"
     CDS             complement(<1..882)
                     /locus_tag="VFT30_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091594.1"
                     /translation="MNPSFSSYLINDVFGDPGVYVEIRWSKRALLFDLGHNEGLGPTR
                     LLRAGEIFVSHTHMDHFIGFDAVLRVTLGRGKTLKLFGPPGLIDNVHGKLRGYTWNLV
                     DGYPLTIDVREFHRNEMQQTVFRASDGFQPSERATIFAPATSGPLFTVLEDPMFTVKA
                     VALNHRIPSFAYSLEEQFHINVNKQKLHGAGLRVGAWLKDVKQYIWQGKPDDFRFTVA
                     LYDEHRGAERDFMLGEVKDRFLTISRGQKIAYVVDARYDEENEAKIVVLAHGADVFYC
                     ESPYLDCDAEKAYGRYHL"
     gene            complement(918..1625)
                     /locus_tag="VFT30_02870"
     CDS             complement(918..1625)
                     /locus_tag="VFT30_02870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5091595.1"
                     /translation="MASGPDRPGPGQVSNVRAALPLLSIGTTFGYYWLPNSLREELLV
                     QFVPQLLAYLAMSFWASRTFQVLAQLGLEKAKVCNGLKWGLITGLLLGCLNTFVILSV
                     YPFFGYDITFLKQTPHGQLPVLLMVPWFICGIALFVELNFRGFLLGRLVELESTWCGT
                     HSIRRFSPLALITSTLTFAFDPFMENTFRYLHWIAVWDGLIWGAIWLRTRNLWITIVA
                     HAVEVIVMYSVVKVAIG"
     gene            complement(1633..>3264)
                     /locus_tag="VFT30_02875"
     CDS             complement(1633..>3264)
                     /locus_tag="VFT30_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5091596.1"
                     /translation="HDRSYQTHYSMLLLRSSIKEASMNRKSSAAPYNLATILIAGLGC
                     ISFPLNSWSLDVTQPITAERREIVSLADAAVRALQSNLDINISRQTKESRLADIIIEQ
                     SKFDPTLSVNGQYNRTVNPLNRPVFGGTLGALDQIRIFDQRNHSVTFDASTNLITGGN
                     FDVNYSPARSNVNQDVARGFLFNPSWTGGLAFTVTQPLLRNAGIEINKTFIKVAQNNA
                     VVEQHVFRDRVLTVITTVEQTYWELVFANENLKVAQAAMKAAEELLATNRAKAKAGVM
                     SIVDVLQAEAAVASRVEQVLVAEKAIRDQEDQLRRLLNPGEEELRQDVGLTPADSPVA
                     YLEPISLQEAIDTAIEQRPEIVQAKKNIESGELNKQFARNQLLPTLSFQGTMGLAGLG
                     GDYGESFTRNFSSDFYNYGAGLVFSYPLGNRSAISTYNKRQLDAKNAEAALANVRQQI
                     IVGVREAVRRVQTDFKRIETTKSARIMAEKQLQAEQERLKVGLSTTRFVLDFQRDLAT
                     AQGNELRAIVDYNKSLSNLARHKATTLDRYHLELS"
BASE COUNT          746 a          865 c          891 g          762 t
ORIGIN      
        1 caaatgatac cggccgtatg ctttttctgc atcacaatcc aaataaggcg actcgcaata
       61 gaagacatct gcgccgtggg caaggacgac gatcttggct tcgttctcct cgtcatagcg
      121 tgcatcgacg acgtaggcga tcttttgccc tcgtgagatc gtcaagaacc gatccttcac
      181 ttctcccagc ataaaatccc gctctgctcc ccgatgctcg tcgtagagcg ccacagtgaa
      241 gcgaaaatcg tccggcttgc cttgccagat atactgcttg acgtccttga gccaagcacc
      301 gacccttagc cccgccccgt gcaatttctg tttgttgacg ttgatgtgaa attgctcttc
      361 cagcgagtag gcgaatgagg gaatgcgatg atttaaggcc accgctttca ctgtaaacat
      421 cggatcctcc agcacggtga agagcggacc gctggtggct ggagcaaaaa tcgttgctcg
      481 ttcggatggc tggaagccgt cactcgctcg gaacaccgtc tgttgcatct cgtttctgtg
      541 aaattcacgg acatcgatcg tgaggggata gccatccacg agattccagg tatagccgcg
      601 cagtttgcca tgaacattgt cgatcagacc tggtgggccg aacagtttga gcgtcttgcc
      661 tcgtccgagt gtgacgcgaa gaacggcgtc aaatccaatg aaatgatcca tgtgcgtatg
      721 agagacgaag atttctccag ccctgagcaa tctggtcggt cccaatcctt cgttgtgccc
      781 gagatcaaag agcaatgctc gcttggacca gcggatttcc acatagacac cgggatcacc
      841 gaatacatcg ttgatcagat aactggagaa ggaaggattc atggagtcat tctatactga
      901 caaccacgac tcacagatca acctatcgcc acttttacga cactatacat cacaatgact
      961 tcgaccgcgt gcgcgacgat ggtaatccaa agatttcgcg ttcgcagcca gatagcaccc
     1021 cagatgagtc cgtcccacac agcaatccag tggagataac gaaaagtatt ctccatgaat
     1081 ggatcgaagg caaacgtgag cgtgctcgtg atcaacgcaa gaggagaaaa acgtcggatc
     1141 gaatgtgttc cacaccaggt cgattcaagt tcgacaagcc gccccaagag aaagccgcga
     1201 aaatttagct ccacgaatag ggcgatgccg cagatgaacc acggcaccat gagcaggacc
     1261 ggaagttgtc catgaggcgt ttgctttaag aaggtgatgt cgtaaccgaa aaagggatag
     1321 accgagagaa tcacgaaggt attcagacat cccagcaaca atcctgtgat cagtccccac
     1381 ttcagcccgt tgcacacctt cgctttttcc aatcccagtt gagccaagac ttgaaacgta
     1441 cgcgaagccc agaagctcat cgccagatac gcaagaagtt gtggcacgaa ctggacgaga
     1501 agctcttctc ggagtgagtt gggaagccag taataaccga acgtagttcc gatagagagc
     1561 agcggcagcg ctgctctcac gttggacact tggcctggac ccggccgatc tggaccggaa
     1621 gccataggac aactacgaaa gttcgagatg ataacgatcc agcgtggttg ctttatgccg
     1681 cgcgagattg gacagtgatt tgttgtaatc gacgatggcg cgcaattcat tgccttgcgc
     1741 ggtggcgagg tcgcgttgga aatcgagcac gaagcgagtc gtgctgagtc ccaccttcaa
     1801 tctctcctgc tcggcctgca gctgcttctc ggccatgatc cgtgccgact tggtggtctc
     1861 gatgcgttta aagtcggtct ggacccggcg caccgcttcg cggacgccga cgataatttg
     1921 ctgacggaca ttcgcaagcg cggcttcggc gtttttggcg tcgagctgcc gtttgttata
     1981 ggtgctgatg gcggaacggt tgccgagcgg atagctgaag actaacccag caccatagtt
     2041 gtagaagtcg ctgctgaagt ttctggtgaa ggactcgccg tagtcgccgc cgagaccggc
     2101 aagtcccatg gtgccttgaa acgatagggt cgggagtaac tgattgcggg caaattgttt
     2161 gttgagttcg cctgattcaa tgtttttttt ggcctggacg atctccggcc gttgttcgat
     2221 cgctgtatcg atcgcttcct ggaggctgat gggctcgagg taggcaacgg gggaatcagc
     2281 gggtgtgagg ccgacgtctt gccgcagttc ctcctcacca gggttcaaga gacggcgcag
     2341 ttgatcctct tgatcgcgaa tggctttctc ggcgaccaac acttgctcca ccctcgacgc
     2401 cactgcggct tcggcttgca gtacgtcgac aatggacatc acccccgcct ttgccttggc
     2461 gcggttggtc gctaacagct cttcggcggc cttcatggcg gcttgcgcca ctttcaagtt
     2521 ctcattcgca aacaccagct cccaataggt ttgttccacc gtggtgataa ccgtcaggac
     2581 gcggtctcga aatacatgct gttcgactac ggcattattc tgggcgacct tgatgaaggt
     2641 cttattgatc tcgatcccgg cgttcctcag taatggttgg gtcactgtga atgccagacc
     2701 gcctgtccag gacggattga ataaaaatcc cctggccacg tcctggttca cattgcttct
     2761 tgccgggcta tagttcacgt caaaattacc gccggtgatg aggttggttg aggcatcgaa
     2821 ggtgacagaa tggttgcgtt ggtcgaagat tcggatttga tccagtgcac caagagttcc
     2881 tccgaacacc ggccgattga gcgggttgac cgtccggtta tattgaccgt tcacgctcaa
     2941 ggttggatcg aacttggact gctcgatgat gatgtcggcc aaccggcttt ctttggtctg
     3001 acggctgata ttgatgtcca gattgctctg cagcgcacga accgccgcat ccgccagtga
     3061 aactatttca cgccgttccg cggtgatcgg ttgggtcaca tccaaactcc agctgttgag
     3121 cggaaacgag atgcacccta agccggcgat caagatggtc gcgaggttgt aaggggcggc
     3181 tgaggatttg cggttcatac ttgcctcctt gatcgacgat cgtaacaaga gcatactata
     3241 atgtgtctgg taggacctgt catg
//
LOCUS       DASTWE010000265         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_556821, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000265 DASTWE010000000
VERSION     DASTWE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556821"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1520)
                     /locus_tag="VFT30_02880"
     CDS             complement(117..1520)
                     /locus_tag="VFT30_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU5091597.1"
                     /translation="MTEKRQVLQKQRGKEPTITSNGYAQLAADVLAHAKACGATEADI
                     VVADGETFSVQVRVGTVDRLTKAREKRLGLRVFIGKRSATTSTSDFTQESLERLVADT
                     CTLARAVVEDDVSGLPDAAQMATEQPSLDLYDETVLDTETQIDWAKRGEAAAFAADSR
                     VTNSEGAEFDSSSGRVVLANSHGFVGSYKSSNFSLSVSPIATESETGAMQRDAWYEVQ
                     RKFARLASAESIGQEAARRAVRRLGARKVATKRVPVVFDQETAGSLLANLCSGVSGYG
                     LYKRASFLLDKLGQSIACDLMTVYDDGRMVGGLGSRPFDGEGLATRKNTIVERGVLKS
                     YLLDTYSGRKLGLPSTGNASRSVGESPSVGPTNFYLVPGLKGPHEIIGSVKEGLYVTE
                     LIGFGINMVTGDYSRGACGFWIENGELAYPVEEITIAGNLKQMFKDIEIIGSDLVFRG
                     RIASPTVKISEMMVAGN"
     gene            complement(1599..>2008)
                     /locus_tag="VFT30_02885"
     CDS             complement(1599..>2008)
                     /locus_tag="VFT30_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU5091598.1"
                     /translation="GNGRRENYQSVVLPRMTNTFMLAGESDPQDIIRSVKKGLYAVSF
                     GGGQVDITNGKFVFSASEAYLIEDGQITTPVKGATLIGNGPEILTKVSMVGHDLKLDN
                     GIGTCGKDGQSVPVGVGLPTIKIDEITVGGTQR"
BASE COUNT          415 a          635 c          493 g          465 t
ORIGIN      
        1 ggtcatacgc atctgtgaac ctcacaagcc atggctcttt acgaccagcg catttggcga
       61 ataccggcat cttccatcgc ttactatgaa accttggtgg cgtttgatca catctcttaa
      121 ttgcctgcca ccatcatctc ggaaattttc actgtggggc tagcaatacg tcctcgaaat
      181 acaagatcgc ttccgattat ttcgatatcc ttgaacatct gtttcagatt gccggcaatg
      241 gtgatttctt caacggggta ggccagctcc ccattttcga tccagaaccc gcaggcgccg
      301 cgcgagtaat cgccggtcac catattgatt ccgaacccga tcaattcggt gacatagagc
      361 ccttccttga ctgaaccgat aatctcgtgc gggcccttca acccggggac aagataaaaa
      421 ttcgtcgggc cgacggaagg actctcgccc acgcttcgtg acgcattgcc agtcgatggc
      481 agccccagtt tccttccaga ataggtatcg agcaggtaac tcttcaatac accgcgttcg
      541 acgatcgtat tcttccgcgt ggccaatcct tcgccgtcaa atgggcgaga tccgagacct
      601 ccgaccattc ggccgtcgtc gtacacggtc atcagatcgc acgcaatcga ctggcccagt
      661 ttgtcaagaa ggaacgaagc acgcttgtag agaccatagc cggacacgcc actgcataag
      721 tttgccaaca ggctcccggc cgtttcttga tcgaacacga ccggcacccg cttcgtcgcc
      781 accttgcgcg cacccaggcg acgaacggct cttctggccg cttcctgccc gatcgattcc
      841 gcggatgcca accgtgcaaa cttgcgttgg acttcgtacc aggcatctcg ctgcatggcc
      901 ccggtttccg actcggtcgc aatcggagat acggacagtg aaaaatttga actcttgtag
      961 gacccgacga atccgtggct gttcgccagc accactcgcc cggacgacga gtcgaactcc
     1021 gcaccttcag agtttgtgac gcgtgagtcg gcggcaaagg ccgccgcttc tccgcgcttg
     1081 gcccaatcga tctgcgtttc cgtatcgagc accgtctcat catagagatc gagactcggc
     1141 tgctcggtgg ccatctgggc cgcatccggt aatcctgata cgtcatcctc aacaaccgcc
     1201 ctggccaagg tgcacgtgtc tgcaacaagc cgttcgagag actcttgggt gaagtcggat
     1261 gtggaagtag tcgccgatcg ctttccgatg aagacacgca gccctaaccg cttctctctc
     1321 gccttcgtca atcgatcgac cgtgccgacc cgcacttgga ccgaaaaggt ctctccatcg
     1381 gccaccacga tatcggcctc cgtcgcgccg caggcttttg catgggccag gacatcggcc
     1441 gcgagctgcg catagccgtt gcttgtgatg gtcggctcct ttcctcgttg cttttgaagc
     1501 acctgcctct tctccgtcat gtgtcactgg tcatgagtag atgagaatct gcctctctga
     1561 cttgagcaat gatccatgac tcatggctat tgattctctc aacgttgtgt accgccgacc
     1621 gtaatctcgt cgattttgat ggtcggcaag ccgaccccga ccggcaccga ttgaccgtct
     1681 ttaccacacg taccgatgcc attgtcgagt ttcagatcat gccccaccat cgacacctta
     1741 gtgaggatct ccggcccatt cccgatcaac gtggctccct taaccggtgt cgtgatctgt
     1801 ccgtcctcga tgaggtaggc ctcgctggca gaaaacacga acttcccgtt ggtgatatca
     1861 acctgcccac caccgaacga cacggcatac agcccttttt tcaccgaccg gataatgtcc
     1921 tgaggatccg actcaccggc cagcatgaag gtattcgtca ttcgaggaag caccacgctt
     1981 tgataattct cccgccggcc gttgccgg
//
LOCUS       DASTWE010000266         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_265761, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000266 DASTWE010000000
VERSION     DASTWE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265761"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..>1048)
                     /locus_tag="VFT30_02890"
     CDS             complement(147..>1048)
                     /locus_tag="VFT30_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5091599.1"
                     /translation="DPWTNVAGKYPIGTRVRGRVVSLTDYGAFVELEPGVEGLVHVSE
                     MSWTHEVRHPSRVVAFGDQVEAAVLNVDPASRKISLGMKQTAPNPWDMIEGKYPIGTR
                     IEGKVKSLTDFGAFVGLEEGIDGLIHISDMSWTKHIKHPSELFKKGQKVEAVVLRIDK
                     EKERLSLGYKQLARDPWDEAIPARYHVGDSVTGKVSKVADFGIFIELEGGVEGLIHVS
                     ESSLEPSAKLEEKFKLQDDVTAKIIKVDREERKIALSLRDHQLDWERKQVDDYHSAQG
                     VLDQSLGRAVKQSRKRSQSDDQS"
BASE COUNT          218 a          325 c          240 g          265 t
ORIGIN      
        1 aagagattga cgagaatcaa ggcaccaagt ccagctgcga aaaaccaaaa tgttttccgc
       61 agcagatggc gctttctcgg gtgttccgtc tgtgtcactt catccgccat tgcccatccc
      121 ccgcatcgtc actcaaacaa gccgactcaa ctttgatcgt ccgattgcga ccgtttccgg
      181 ctctgcttga cggcccttcc cagactctgg tccaacacac cttgcgccga gtgatagtca
      241 tccacttgct tacgctccca gtccagttga tggtcgcgaa ggctgagggc tattttgcgt
      301 tcctctcgat cgactttgat tatcttcgcc gttacgtcgt cctgcaactt aaacttttct
      361 tccagcttcg cggaaggctc aagactggac tcgctgacat ggattaggcc ttccactcca
      421 ccttccaatt cgatgaagat tccaaaatcg gcgacttttg acaccttgcc tgtcacggaa
      481 tcgccgacgt gataccgcgc gggaatcgcc tcatcccagg gatcacgcgc caactgtttg
      541 taccccaacg aaagccgctc tttctctttg tcgatgcgca acacgacagc ttcgactttt
      601 tgtccttttt taaacagctc agagggatgc ttgatatgct ttgtccacga catatccgaa
      661 atatggatga gcccgtcgat cccctcttcc agtccgacaa aggcgccgaa atcagtcagg
      721 ctcttcacct ttccttcaat acgggtcccg atcggatatt tgccctcgat catgtcccag
      781 gggtttgggg cagtttgttt catgcccaat gagatcttgc gactcgcggg gtcgacgttg
      841 agcaccgcgg cttctacttg atctccaaac gccacgactc ttgatggatg tcggacttcg
      901 tgtgtccacg acatctccga aacatgcacc agtccctcga ctcccggctc aagttccacg
      961 aacgctccgt aatcagtcag gctgaccaca cgcccacgga ccctggtacc gatgggatac
     1021 ttgccggcga cattggtcca gggatcgg
//
LOCUS       DASTWE010000267         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_879838, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000267 DASTWE010000000
VERSION     DASTWE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879838"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..481
                     /locus_tag="VFT30_02895"
     CDS             110..481
                     /locus_tag="VFT30_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091600.1"
                     /translation="MPSVLVVDDQDQVRQLIRETLEQAGYEVEEACDGKEGLDRYRVR
                     STDLVIMDILMPDQDGLETIMTLRQEFPDIRIIAMTGGSDTVGIPNFLDVAKMLGARR
                     TLQKPFELKVLLEAVAAELTV"
     gene            680..>1079
                     /locus_tag="VFT30_02900"
     CDS             680..>1079
                     /locus_tag="VFT30_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091601.1"
                     /translation="MIFFNLSLGTLVATVGFWLIWDGTVPPIIVFGWALTVGLFLWFR
                     TESITELWAWSTLLLGLESFAWPLVLMIQLRGQAESLPESEMGTILSAVVLGLFSSVF
                     WISFSYGLFKRARKLDSALSVEQTATVPISG"
BASE COUNT          211 a          287 c          313 g          268 t
ORIGIN      
        1 gcgtacgcca tacgccgggc actggatcag tcttctgcct atccggagtc tacgggtgcg
       61 gcgaccaact ccggccagac tccacggagg atgtcgattg aggtgtcgga tgccgtcggt
      121 cctcgtggtt gatgatcagg accaagttcg tcagctcatt cgggaaacgt tggagcaggc
      181 cggctacgag gtcgaagagg cctgcgacgg gaaagagggc cttgaccggt atcgggttag
      241 gtcgacggat cttgtcatca tggatattct catgccggac caagacggat tagagaccat
      301 catgacgctc cgtcaggagt ttcccgatat tcgtatcatt gcgatgactg ggggaagtga
      361 tacggtcggc atccccaatt tcttagacgt ggcaaaaatg cttggagcca ggcggacgct
      421 ccaaaaacca ttcgaactga aagttctgct tgaggcggtt gctgccgaac tgaccgtcta
      481 gctgagtgtc gaaaagtccg ccagccaagt tcttgcgacg gaggacacgg ggcgctcacc
      541 gactcgcgcc cgtccgcaaa cgtggcgctc attattcttc gcgccgcgga cctcgccgca
      601 caacgctcac cattcttcgc attgacatca ggacatggtc ccgtcagact ccgcattcca
      661 tgcctggatc atgcacttca tgatattttt caatctgtcc ctcgggaccc tcgttgccac
      721 agtcgggttc tggctcatat gggatggaac tgtccctccg atcattgtgt ttggatgggc
      781 tctgaccgtg gggttgttct tatggttcag gacggagtca atcacggaac tatgggcatg
      841 gtctaccttg ttgctcggcc tggaaagttt tgcctggccg ttggtgttga tgattcaact
      901 ccggggccag gcagaatcac ttcccgaatc tgagatggga acgatccttt cagctgtcgt
      961 gctgggtctc ttttcatctg tgttttggat ttcgttttcc tacggcctgt tcaaacgagc
     1021 acgtaagctg gattcggctc tatccgtgga gcagactgcg acagtgccga tttcagggg
//
LOCUS       DASTWE010000268         1485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1106236, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000268 DASTWE010000000
VERSION     DASTWE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106236"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1196)
                     /locus_tag="VFT30_02905"
     CDS             complement(303..1196)
                     /locus_tag="VFT30_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU5091602.1"
                     /translation="MAAGIVSSAATAVGIATGNGLFGFERRADAKFSLTVQTGDATGW
                     SATVHRSIDHLKVQERTLAAIAKAKRGRSAREMPPGRYPVILEPAAVAGLWAWLISAL
                     DAKSYVKRTSPFAGKLGQAIVDERLTLRNVPDHADLLGEGFTHEGLPSTDSVWIDQGI
                     LKQLAYDRFTAKAHGVKTIPTLEAPALSVEGPPAHSIQDLIKGTEHAILVSNFWYIRP
                     VNPRDLMLTGMTRDGAFLVEKGEIVSAVKNFRFHESPLQVFRRINMWTSPMEAVNSET
                     GKMLVPAMALPQFYFSSVTRF"
     gene            complement(1227..>1485)
                     /locus_tag="VFT30_02910"
     CDS             complement(1227..>1485)
                     /locus_tag="VFT30_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU5091603.1"
                     /translation="QNVDARRGSLAVTVGFGGRHGTASTTDFTAGAIQDALARAERIA
                     RISPVDPEYLPPPDPCVFPIRETAKQETIAAGPVKRLEYAN"
BASE COUNT          316 a          440 c          405 g          324 t
ORIGIN      
        1 cgtcctcata ccgcgtcacg tacgcgaaga gatcaaccat cttgcgccaa caagcagctc
       61 cggcatcgag caggagctga ccatagggtg caggcaccgg acgagcttca tcgatttgct
      121 tctgcgaagg aattcgatga atgaggttga tcggttcttt gttgaatcgc ggggccatct
      181 ggcgaatccc cttcattcgg acctagtatg ttactactga tgtacctact cccccgagaa
      241 tggaagagtc aaccctttta ttatgtggac gatgaaatat attcggagtc cttatgataa
      301 ttctaaaacc gggtcacgct ggagaaataa aattggggca gggccatagc cggaaccagc
      361 atcttcccgg tttccgaatt gaccgcctcc atgggcgagg tccacatgtt gatgcgtcga
      421 aatacctgga ggggactctc atggaagcgg aaatttttca ccgctgagac gatctctccc
      481 ttctcgacga ggaatgcccc atctcgtgtc atgccagtca gcatgagatc gcgaggattc
      541 acggggcgga tgtaccaaaa attcgacacg agaatcgcgt gttccgtacc tttgatgaga
      601 tcctgaatcg aatgtgccgg aggtccttcg accgacaagg caggggcctc caaggtcgga
      661 atggttttca ctccatgcgc cttcgccgta aatcgatcgt aagccagctg cttgaggatc
      721 ccttgatcga tccacaccga atcggtactc ggcaacccct catgggtaaa tccctctccg
      781 agcaggtcgg catgatccgg aacgttccgg agggtcagcc gttcgtccac gatcgcttgt
      841 cccagcttgc cggcgaacgg gctcgtcctt ttcacgtaag acttggcatc cagtgccgaa
      901 atcagccatg cccaaaggcc ggccacggct gccggctcaa gaatcacagg ataccgcccc
      961 ggcggcatct cgcgcgcaga ccgacctcgt ttggctttgg caatggccgc caatgtccgc
     1021 tcttgaacct tcaagtgatc gatcgaccga tggacagtcg cgctccaacc ggttgcatca
     1081 ccggtttgga ccgtcaggct gaacttcgca tccgctcgac gttcgaagcc gaatagtcca
     1141 ttgccggtgg caatccctac tgccgtcgcc gccgacgaga cgatgccggc cgccatgagg
     1201 ttctccatcc ggcagtgtcc gatcgcttaa ttggcatact ccagacgttt gactggccct
     1261 gccgctatgg tctcctgttt tgccgtttct cgaatcggga acacgcacgg atcgggaggc
     1321 ggaaggtatt cgggatcgac cggtgagatt cgagcgatcc gttcggcacg ggccaacgcg
     1381 tcctgaatcg caccggcggt aaaatcggtc gtgctggcgg tcccatgccg acccccgaag
     1441 ccgaccgtca ccgccagaga gcctcgtctc gcatcgacat tttga
//
LOCUS       DASTWE010000269         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1590996, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000269 DASTWE010000000
VERSION     DASTWE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590996"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFT30_02915"
     CDS             <1..195
                     /locus_tag="VFT30_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091604.1"
                     /translation="AEGRADAKALRCRAKRYLVLTDQMVLALSDLFESIAEDPTAWAQ
                     DVRNFLPEWLQKEPSAIGRS"
     gene            207..911
                     /locus_tag="VFT30_02920"
     CDS             207..911
                     /locus_tag="VFT30_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEU5091605.1"
                     /translation="MVSVVIPTYNEEKALPETLQVLFRQPGDYEVIVVDGGSTDRTSA
                     IVSEFALSSQSSALSTVRLLSSPKGRAIQMNAGAKVANGEWLLFLHADTLLPACAMQR
                     LSQLEADPKIQAGGFMHQFSGDDWRLRCISFLDNFRCVRSRIIYGDQALFVRREFFER
                     LGGFPNQPILEDVAFCEKVLKETDPLLLFPPVITDARKFIKMGIFRSFVRVLLIILHV
                     EFRLPVLPHTFFQDIR"
BASE COUNT          229 a          292 c          293 g          275 t
ORIGIN      
        1 gccgagggac gagccgatgc aaaggcgctg cgttgccggg ccaagcgata cctcgtcttg
       61 acggatcaga tggtgctcgc attgagcgat ttgtttgagt ctattgcgga agacccgaca
      121 gcatgggctc aagacgtacg gaactttctg cccgagtggt tgcagaaaga gccgagtgct
      181 ataggtagat cataaaggca ggaagcatgg tctccgttgt catcccgacc tacaacgagg
      241 aaaaggcgtt gccggaaacc ctgcaggtgc tctttcggca acccggggac tatgaggtga
      301 tcgtcgtcga tggcggcagt accgatcgca cgagcgcgat tgtctcagag ttcgcgctca
      361 gttcacagtc ctcagcactc agcactgttc gtcttctctc ctccccgaag ggtcgtgcca
      421 tacaaatgaa tgcgggagca aaggtcgcca acggcgagtg gctattgttc cttcacgccg
      481 acactcttct gcccgcctgc gccatgcagc ggttgagcca attggaagca gatcccaaga
      541 tccaagccgg tgggttcatg caccagtttt caggggacga ctggcggctc agatgtatct
      601 ccttcttgga taatttccgt tgtgtcagaa gtcgaatcat ctacggtgat caagccttgt
      661 tcgttcgtcg ggaattcttt gaacgactcg gcgggtttcc caatcagccg attctcgagg
      721 atgtcgcctt ctgtgagaaa gtcctcaagg agacagaccc attgctcctc tttccccctg
      781 tcatcaccga tgctcggaaa tttatcaaaa tgggtatctt tcgaagcttc gttcgcgtcc
      841 tgctgattat ccttcatgtt gagtttcgcc tgccggttct ccctcatacg ttctttcagg
      901 acatccgcta atttattctc tttactggtg gcgagactgt cattcaccag gcctccgttc
      961 accttttcct actcctctcc ggttaacgct ccagagaaga gtgtcggaga aaagttcgtc
     1021 aagttgatcg cagtgaagat tggcgagaac atctgcagat gaatgccgat ggtgaattgc
     1081 cggggctcg
//
LOCUS       DASTWE010000270         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_233808, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000270 DASTWE010000000
VERSION     DASTWE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233808"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFT30_02925"
     CDS             complement(<1..576)
                     /locus_tag="VFT30_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU5091606.1"
                     /translation="MALWSRSHSLQQKIIAAIVMVGLLPLTLLLALIYIEERRALRES
                     TGANFKEVAIEAARRIEIHITRSMNEAQQLATAPFLRTAVSEANRTYEGKDAQGISEI
                     INDWQRRWGQRDKRSEFPLFVNRIVTNYLIRWHEIRKSDYVGILVTDGQGALVVSSIP
                     QVEYSYAKTVWWQAVIKGGSQQSYVGEIAFDP"
     gene            complement(719..1696)
                     /gene="secF"
                     /locus_tag="VFT30_02930"
     CDS             complement(719..1696)
                     /gene="secF"
                     /locus_tag="VFT30_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5091607.1"
                     /translation="MKPSAISLQGETQGYQKGARMLEILGKTNIDFMGKRKFSFLFSG
                     IMVLLGLIALVQIARGAANLGIDFAGGTAVQLKFEQPVRIDEARKALETNGLADAELQ
                     EFGQDNKLLIRVKASTTIEEKTAERVVGVFAKEFPANKFVVDSTTEIGPTIGKKLQED
                     ALVAIVISFAGIILYIAARFELRFGVAAALATFHDVLAVVGAFYILDKEITLLIVTAL
                     LTLAGYSLTDTVVVFDRIRENLKLRRRESEEATINSAINQVLSRTIVTSLTVVIVLIP
                     LTLAGGEVLHDFSLALLWGVIFGTYSSVFVASPLLLLWPGAAGRLLKRG"
     gene            complement(1699..>2014)
                     /locus_tag="VFT30_02935"
     CDS             complement(1699..>2014)
                     /locus_tag="VFT30_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5091608.1"
                     /translation="LTIGMGVDSNVLIFERIREELRGGKAPRSAVDAGYDKALLTIID
                     SHVTTLITGVALFLFGTGPIKGFAVTLCLGIAINLFTALVGTKVIFDLLYHRQKVEAL
                     SI"
BASE COUNT          480 a          571 c          516 g          447 t
ORIGIN      
        1 tgggtcgaag gcaatttcgc ccacgtaaga ttgctggctg ccgcctttta tcacagcctg
       61 ccaccagacg gtcttggcat aggaatactc cacttgaggg atcgaactga cgaccaatgc
      121 tccctgtccg tcggtgacca aaataccgac gtagtccgac tttcgaattt cgtgccatcg
      181 aatcaagtaa ttggtcacga tccggttgac aaagagagga aattcgcttc gcttgtcgcg
      241 ctgcccccac cgccgttgcc aatcattgat gatttcagaa ataccctgag catctttgcc
      301 ctcgtacgtt cggttggcct ccgagacggc ggtgcgaagg aagggtgccg tcgcaagttg
      361 ctgggcttcg ttcatacttc gagtaatatg tatttcgatt cgacgggcgg cttcgatggc
      421 tacttctttg aaattcgccc ccgtcgactc tcgcaaggca cgtcgctctt ctatatagat
      481 gagggcaagc aagagagtga gcggcaggag accgaccatc acgatcgctg cgatgatctt
      541 ctgctggagg ctgtgagacc ggctccagag tgccatggct acaccagaga gacgtaagaa
      601 cggtgtttcc ttgtgagaaa gcgttcgcgt tgtaagtcga cgatgggact caatacccac
      661 tcaccaccga cagcgcgggt cgaacgaaca acgcgcggcg ctctattcca tcatttgctc
      721 agccgcgttt caagagtcga cccgccgctc ccggccacaa gagcaggagt gggctggcaa
      781 cgaataccga agaataggtg ccgaaaatca caccccaaag cagcgcaagt gagaaatcgt
      841 gcaggacttc tccgccggca agagtcagag gaataaggac aatcacgacc gtcaaactcg
      901 tcacgatcgt gcggctcaac acctgattga tggcgctgtt gatcgtcgcc tcttcgcttt
      961 ctcgacgccg caatttcaga ttttctctga ttcgatcgaa gaccacgacg gtgtcggtca
     1021 aagaatatcc ggccaacgtc aggagcgccg tcacgatcag gagggtaatt tctttgtcta
     1081 agatatagaa ggccccgacc acggccaaga catcatgaaa cgtcgccaac gcagcagcca
     1141 cgccgaaccg gagttcgaat cgtgccgcaa tgtacagtat aatacccgca aatgagatga
     1201 cgattgctac aagcgcatct tcctgaagct tcttgccaat ggtcggtccg atctcagtgg
     1261 tcgagtcgac cacgaatttg ttggcaggaa actctttggc aaagactccc accacgcgtt
     1321 ccgccgtctt ctcttcgatc gtcgtggagg ccttcacccg gatgagaagt ttgttgtctt
     1381 gtccgaattc ctgcagctcc gcatcagcca gtccgttagt ctccagggcc ttgcgggcct
     1441 catcgattcg aaccggctgt tcgaacttga gctggacagc tgttcctcca gcaaagtcga
     1501 tgcccagatt agcggcaccc cgtgcaatct ggacgagtgc gatgagccca agcaatacca
     1561 tgattccaga aaagagaaag gagaacttgc gcttgcccat gaagtcaatg ttggtcttcc
     1621 cgagaatctc taacatgcgc gctccctttt gataaccctg agtctcccct tgaaggctga
     1681 tcgctgatgg cttcatggtt agatgctcaa cgcctcgact ttttgtcgat gatacaacag
     1741 atcaaaaatc acttttgtcc cgaccaatgc cgtgaagagg ttgatggcaa tgcccaagca
     1801 cagcgtcacg gcaaacccct tgatcggccc ggtgccaaag agaaacaggg cgaccccggt
     1861 gatcagcgtc gtcacgtgtg aatcgatgat cgtcaataac gccttgtcgt aacctgcatc
     1921 gacggcggat cgcggcgctt ttccgccacg caactcttcg cgaatacgct caaaaatcaa
     1981 gacgttcgaa tcgacgccca taccaattgt cagc
//
LOCUS       DASTWE010000271         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_847603, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000271 DASTWE010000000
VERSION     DASTWE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847603"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..>1142
                     /locus_tag="VFT30_02940"
     CDS             138..>1142
                     /locus_tag="VFT30_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091609.1"
                     /translation="MIENSIEMWPIINDILRREGVARQHLNSYNEFMERGLQSIIDEV
                     NEIEIETAEYPYKIKLGKVKLQQPRIMELDGSITHVAPMEGRLRNLTYGSPIMLECSV
                     VEDGKILESRFIHIGDMPVMVKSNTCLLHNLPEMKLVEFGEDPRDPGGYFVINGSERV
                     IVGLEDLSYNKIIVDTEETTGTLLYKAKVYSSIVGYRAKLELIMRPDGSIVTKIPGSP
                     VDIPLITLIRALGLEADKDIADSVSLTDLIQDELEPSFEKAGDVASSRDAIVYISKRI
                     APGMLEEFQIKRAETLLDWGLLPHIGKNPENRHEKALFLGEAASKLVELKLGWIESDD
                     K"
BASE COUNT          307 a          299 c          314 g          222 t
ORIGIN      
        1 ccgtacatcc tcatgagcga caaaggcatc gaggacttgg acgtgaggcc cggagacgtc
       61 atcaagatca cgagaaagag cccaactgca ggtcaaagtg tatattaccg ctacgtggtt
      121 gaagggtgag aagggcaatg atagagaatt ctatcgagat gtggccgatt ataaatgaca
      181 ttttgagaag ggaaggagtc gcaagacagc accttaattc ttacaacgag ttcatggagc
      241 gcggactcca gagcattatc gacgaagtca acgaaattga aattgaaacc gcagaatacc
      301 cttacaagat aaagcttggc aaggtaaaac tgcagcagcc caggataatg gagcttgacg
      361 gctctatcac acacgtcgcc ccgatggaag ggaggctgcg caaccttacc tacggttcgc
      421 ctatcatgct ggaatgctcg gtcgtcgagg acggcaagat actggaatcg cgctttatcc
      481 acataggcga catgccggtg atggtcaaat cgaatacctg cctcctgcac aacctgccag
      541 agatgaagct ggtcgagttt ggcgaggatc cgcgcgaccc cggcggatac tttgtcatca
      601 acggctcaga gcgcgtcatc gtgggccttg aggacctttc ttacaacaag atcatcgtcg
      661 acaccgaaga gactaccggc accttgctct acaaggccaa ggtatattca tccatagtcg
      721 gctatcgcgc aaagctggag ctcatcatga ggccggacgg ctcgatagtg acaaagatac
      781 cgggctcgcc tgtcgacatc ccgctcatca cgctcatccg ggctctgggc ttggaagctg
      841 acaaggacat cgccgactcg gtgtcactca ctgacctcat ccaggacgag ctcgagcctt
      901 cgtttgaaaa ggcaggcgac gtggcttcaa gccgcgacgc aattgtctac atttcaaaga
      961 gaatcgcgcc cggcatgctt gaagaattcc agatcaagcg cgccgagacg ctgcttgact
     1021 ggggcctctt gccgcacatt ggcaagaacc ccgagaacag gcacgaaaag gcgctgttcc
     1081 ttggcgaggc agcaagcaag cttgtcgaac tgaaactggg ctggatagag tcagacgaca
     1141 ag
//
LOCUS       DASTWE010000272         1163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1461667, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000272 DASTWE010000000
VERSION     DASTWE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461667"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="rph"
                     /locus_tag="VFT30_02945"
     CDS             complement(<1..674)
                     /gene="rph"
                     /locus_tag="VFT30_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5091610.1"
                     /translation="MKVTRNFIKHAEGAVLIEMGDTKVICTASVEEKVPPFLKGKGTG
                     WVTAEYAMLPRATHERSPREAVKGKQGGRTLEIQRLVGRALRSVTDMSQLGERSIWID
                     CDVIQADGGTRTASITGAFIALADACAVLKKRDLLKKIPLTDYLAAISVGKVGGEVMV
                     DLAYTEDSMAEVDMNLVMTGRGRYVEVQGTAERTPFAKQDMDDFLNLGWQAIQRLTAI
                     QKELIGA"
BASE COUNT          282 a          349 c          262 g          270 t
ORIGIN      
        1 gcaccgatca gctctttctg aatggcagtc aaccgttgga tggcctgcca gccgagattc
       61 agaaaatcat ccatatcttg cttggcaaac ggcgtacgtt ccgccgtacc ctgtacctcg
      121 acataccggc cgcggccggt catcaccaaa ttcatatcca cttccgccat tgaatcctcg
      181 gtataggcaa gatccaccat cacctctcca ccgacctttc cgacactgat ggcggccaga
      241 taatcggtga gaggaatctt tttcaagaga tccctttttt tcagcacggc acaggcatca
      301 gccagtgcga taaaggcgcc ggtgatggac gcggttctgg tgcccccatc cgcttggatc
      361 acatcacagt ctatccaaat ggaccgttct cccaactgag acatgtcggt cacggaccgc
      421 agagcgcgtc cgacaagacg ttgaatctcc agggttcggc caccttgctt tcccttgacc
      481 gcctctcgtg gcgatcgctc atgggttgca cgcggcaaca ttgcatattc cgccgtaacc
      541 catccggtcc ccttgccctt gagaaaaggc ggcacctttt cctcgacgga tgccgtacaa
      601 atgaccttcg tgtctcccat ttcaatcagc acagcccctt cggcatgctt gataaagttg
      661 cgtgtcactt tcacggggcg gacctgatct ctacgacgcc catcgaaacg gactagcccg
      721 gaaattccgc tcattcttac gccccttcct caatagtggg atccaaatta tagtgaaggc
      781 cttacgaaag cgcaaaccag ctgtccaacc gatcatcgtc ggtgaatcga tgcgtcgacc
      841 tattgctcta cgacctttat cctcatttgt acaaaagcag gtcgtcgagt ttgacgaatt
      901 ctgtacaatc ttttgacgta tcgtccttct tcggaataga acaaagtaag agtctatgac
      961 aaggtatctc tgaaaatccc tccagacgcg gagattccgt ctcgctttct tgagatcatc
     1021 gaaatggacg gtgtaacaat gccagcgccg gcgcaacagc aattgggcac aaaaagtgca
     1081 acctctgcgc cgttcctacg gcattcagat taaccaccaa tgagccgata aagaagaagc
     1141 gagacaatcc gcgacatcga caa
//
LOCUS       DASTWE010000273         1852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_783084, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000273 DASTWE010000000
VERSION     DASTWE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783084"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFT30_02950"
     CDS             <1..760
                     /locus_tag="VFT30_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5091611.1"
                     /translation="PVFMILFSFLLMGLVGCAAHHANEPDRQKQERANELDPTTPTIE
                     ATSDEEDRQSIQQGKALFHGKAACFECHGENGDISRVSHSQLARLNPPPTDLRTPTDK
                     SLRQLYLIIKYGISATGMIPIQEVAKLDDEEILHVISYLSYLQGRSPSFDLISSQSFL
                     PHTDTDVAISLLCEQEALANVDKEEQCEDRYAKRYCDLLIGRPPDIPIARYTEIETGC
                     KERAAKDLDTLALCYRAEYTASRQATQKLGNQQP"
     gene            944..1468
                     /locus_tag="VFT30_02955"
     CDS             944..1468
                     /locus_tag="VFT30_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091612.1"
                     /translation="MDILILCLIVLMGWAAWIAFRHSSRRRTVNANPHHHDGSSQKRA
                     ALRDKPAELVNMPESHSRPLTSDDEHHAFKVGQPIWIRYEDKSGMVTERVVEIYRPSD
                     DEVIYTWCRRKQAPRTFARRNIRNWRLLPERFDFDPIVAQYWDEEGTRDRSEKNPWRR
                     WLRRQPKEIANRYK"
BASE COUNT          475 a          494 c          482 g          401 t
ORIGIN      
        1 accagtattt atgattctgt ttagttttct cttaatggga ttagtgggat gtgcggctca
       61 tcatgcaaac gagcccgatc gacagaaaca ggagcgagca aacgaacttg atcctacgac
      121 accaaccatt gaggcaacca gcgacgagga agaccgacag tctatccagc aagggaaagc
      181 gctctttcat ggcaaggccg cgtgttttga atgtcatggg gaaaatgggg atatcagccg
      241 cgtatcccat tcacagctcg cgaggttaaa cccaccccca accgatctac gaacgccaac
      301 tgataagtcc ctgcgtcaac tgtatctgat catcaagtat ggcatttcag cgacgggaat
      361 gatccccatc caggaggtgg ctaagctcga cgatgaggaa attctccatg tcatttccta
      421 tctctcatat ttacaagggc gatcaccttc gttcgatctg atatcaagtc agagttttct
      481 cccgcatacc gacactgatg tcgcgatttc actactgtgt gagcaagaag cccttgccaa
      541 tgtcgacaaa gaagaacaat gtgaggatcg ctacgccaaa cgatattgcg atctccttat
      601 aggacgtcct ccagatattc cgatcgcccg atacacggag attgaaaccg gctgcaagga
      661 gcgagctgcc aaggatctcg acacattagc gctctgttac cgagcggagt atacggcatc
      721 gagacaggca acacagaagt tgggcaatca acagccctag taggctgcag aaacttcctc
      781 ctattcctcc gcaacgcaaa ggacttgctg aagaagtaca gagagaaatg aagatagagg
      841 acctgcagtt tcttgggccg acgaggtatc ggggagagct cccttgtaga ttggatctga
      901 gacctactgc actctgacga cgaatgcccg ctggtaagct cgcatggata ttctcattct
      961 gtgtctcatt gtcttgatgg gctgggctgc atggattgca tttcgccact catcgcgcag
     1021 gcgcacggtc aacgcgaacc cgcatcacca cgacgggtca tcgcagaaaa gggccgccct
     1081 cagagacaaa ccggctgagc tcgtcaacat gccggagtcg cacagccggc cattgacatc
     1141 cgacgacgag caccacgcat tcaaggtcgg ccaaccgatc tggatccgtt acgaagacaa
     1201 atccggcatg gttactgagc gagtagtcga gatttaccgt ccaagcgatg acgaggtgat
     1261 ctacacctgg tgccggagga agcaggcccc gcgcaccttc gcccggcgaa acattcgcaa
     1321 ctggcggctg cttcccgagc gtttcgactt tgatccgatc gtcgcccaat actgggatga
     1381 ggaagggact cgcgatcggt cagaaaaaaa cccgtggaga cgctggctgc ggcgccagcc
     1441 gaaggaaatc gccaaccgtt acaagtaatt aagctcaacg tctccgattg attttgtggt
     1501 gccatttgta tgagcggctt tttacaccac gatttatagg caatgcaata atagccttga
     1561 gcggcctggg agtcgtaggg tacttagact gcttgaactc ccccttggaa aggaatgctc
     1621 catgcactgg aaaccatgtt gcaaacgtcc tcagatcccg ttggggacac agttcttttg
     1681 cacgaccagt catcagccga ccgatcacgg cagtcagtcg atatgagtta agggaatcct
     1741 atgaaggaac cttcgttcgt gggcgacttt aagggctccc acaagagcag ggatatctcc
     1801 tccagccgtg atggccgata ccaatcgttt agcttcggag tcgaccgtgg cg
//
LOCUS       DASTWE010000274         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_104321, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000274 DASTWE010000000
VERSION     DASTWE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104321"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..>1195)
                     /locus_tag="VFT30_02960"
     CDS             complement(130..>1195)
                     /locus_tag="VFT30_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEU5091613.1"
                     /translation="FETRAQEMEEALVPHAKLSKTQRGLIGAYFLNEYSFEASALFNP
                     SIVRHPDQAGAPENGCRFILSLRAVGEGHISSLTFRSGSINADGSVAVDQTARLASVP
                     KVHSRTSGPHGHDVEVIFRPDEELSERVIFPITDAQANGIEDARFVEFNDGTQRTYYA
                     TYTAYSGRAIRSELLETTDFRSFRMTPLLGSAARNKGMALFPRKIDGRYAMIGRQDNE
                     NLYLIYSDDLYSWEGGQPILQPEYPWEFVQIGNCGSPIELDDCWLLLTHGVGPVRKYS
                     IGAVLLDKKDPSKVLARSREPLIRPEPTEREGYVPNVVYTCGAMRHRERIILPYAISD
                     TFSEFATLEISTLMQALASS"
BASE COUNT          262 a          334 c          340 g          259 t
ORIGIN      
        1 caggatgagc ggcccggttg tgatcactag ctcgatgatg ccgcgcgcga ggctcggcgt
       61 gcgatatcga gcgagaatct tcattcagcg gcgcgcatca gtggtgtcag gggccgacct
      121 gacgcgcatc tatgagctcg caagggcttg catcaatgtg gaaatctcca gcgtcgcgaa
      181 ctccgaaaag gtgtcagata tcgcataagg gagaatgatc cgttcgcggt gacgcatcgc
      241 gccgcacgtg tagacgacat tgggcacgta accctcgcgt tccgtaggtt cgggacgaat
      301 cagaggttcg cgcgaacgtg cgagaacctt tgagggatcc ttcttatcaa ggagaacagc
      361 cccgatcgaa tattttctga ctggaccgac cccatgtgta agcaacagcc agcagtcatc
      421 caactcgata ggtgagccac aatttccgat ctgcacaaac tcccaagggt attcgggctg
      481 caggatgggt tgaccgcctt cccacgaata aaggtcgtca gaatagatca gataaaggtt
      541 ctcgttgtcc tgccggccga tcattgcata gcggccatcg atctttcgtg gaaacagggc
      601 catgcccttg ttgcgagctg ccgatccgag aagcggcgtc atccggaacg accggaagtc
      661 ggtcgtttct aacaactcag agcggatcgc cctcccgctg taggccgtgt aggtcgcata
      721 gtaggttctt tgtgtaccgt cattgaattc cacaaagcga gcatcctcga tgccgtttgc
      781 ttgggcatcg gtgatcggaa agatgacccg ctcgctcagt tcctcgtccg gccgaaagat
      841 gacttcgaca tcgtgcccat ggggccccga tgtccggctg tggactttcg gaaccgacgc
      901 gagacgtgct gtctgatcga cagccacact tccgtctgca tttatgctgc ctgaccggaa
      961 cgttagcgat gagatatgac cttcgccgac ggcgcgaagg ctcaagatga accggcagcc
     1021 attttccggc gcgccagcct ggtcgggatg ccggacaata ctcggattga ataacgccga
     1081 tgcttcgaac gagtattcgt tgagaaaata tgcaccgatc aagccacgct gcgtcttact
     1141 caatttcgca tgtggtacga gcgcctcctc catctcctgg gcgcgtgtct cgaaa
//
LOCUS       DASTWE010000275         1735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1397250, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000275 DASTWE010000000
VERSION     DASTWE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397250"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1735
                     /locus_tag="VFT30_02965"
     CDS             <1..>1735
                     /locus_tag="VFT30_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091614.1"
                     /translation="KLILNIAIAIGILAASLFLLSTIPADKLTTALAGLTGIVVLMTV
                     TMKQMGKIVEDLDGKKVNLKMIALGIAIAALGFAMMELAVAMKIMDSVDMDGIAKGLG
                     TMFVTMKLMQSLGTLGQHAAKSMVGGAAAIAIVAGSMVLLAGALLLFKMVDWGSMAKA
                     GVALAGLTLAVGALAMIPYAGIAKVGLALLGASAGMLAIANALILFQFVNWESIAKAG
                     VVLLMLTASLAALMFVAQPMSVGMFLALGAAMLYLSFALKNLNDVEWASVGKLAVVLT
                     ILIVAVIALTGALILLAPVVPVLALFAAALLALGAALFLFAEALSIAMALAAGGAAAF
                     AALATGAAVAVAVFFQTLAHEAPLMKKAFLKILEELIDGIVKAVPMIIDGIQRLWNAV
                     VKELGGGGGKGGGGSKGAQMNAAGGSWISNLADGIRNKLPEITKKAGELIVGFLLGLR
                     QKAADIAEAGVALIVSIINGISRKIGDLVTAAYNLVIKFAEGVRKNVNKLIDAGIDLI
                     ADFLHALADSIRNGSAAIGSGIRDVVDAFGDVGRDMIGGLIDGIESMFDDVVGAVTSV
                     VGELPGWAKKVL"
BASE COUNT          335 a          449 c          535 g          416 t
ORIGIN      
        1 aagctgattc tcaacatcgc tattgccatc ggaattctgg cggcatcact cttccttctt
       61 tcgactattc ctgccgacaa gttgactacg gctctggccg gtctgaccgg catcgttgta
      121 ctcatgacgg tgacgatgaa gcagatgggc aagatcgtcg aggatctcga cggcaagaag
      181 gtcaacctca agatgatcgc gctcggcatc gctatcgcag cccttgggtt cgcgatgatg
      241 gagctggctg tcgcgatgaa gatcatggat tccgtcgaca tggacggcat cgccaagggt
      301 cttggcacca tgttcgtcac gatgaaactc atgcagagtc ttggaactct cggtcagcac
      361 gcagcaaaga gcatggttgg tggcgcagca gcaatcgcca tcgtcgctgg atcaatggtc
      421 ctgctcgctg gtgcactcct tctgttcaag atggtggact ggggttccat ggcaaaggct
      481 ggcgtagctt tggccggtct gactctcgca gtcggtgccc ttgcgatgat cccgtacgcg
      541 ggtatcgcca aggttggtct ggcccttcta ggggcttcgg ccggaatgct cgcgattgcg
      601 aatgccctga ttctgttcca gttcgtcaac tgggagtcca tcgccaaggc cggcgtcgta
      661 ctcttgatgc tcactgcgtc tttggccgct ctgatgttcg tcgcacagcc gatgagcgtt
      721 ggaatgttcc tggccctcgg tgcagcgatg ctatatttgt cgttcgccct caagaacctg
      781 aatgacgtcg agtgggcgtc tgtaggaaag cttgcggtcg tcctgaccat tctcatcgta
      841 gcggtaatcg cactcacggg cgcgctgatc cttctggcac ctgtcgtccc ggtcctggct
      901 ctcttcgctg cggcgttgtt ggcgctggga gcagcactat tcttgttcgc agaagcgcta
      961 tcgatcgcga tggctttggc tgcaggtggt gcggcggcat tcgctgcttt ggccacaggt
     1021 gctgcagtag cagtagccgt gttcttccag acactggctc acgaagcgcc tctgatgaag
     1081 aaggcgttcc tcaagatcct cgaagagctc atcgacggca tcgtcaaggc cgttccgatg
     1141 atcatcgatg gcattcagcg actctggaat gctgtggtga aggagctcgg cggtggaggc
     1201 ggcaagggcg gcggaggttc caagggcgcc cagatgaacg ctgctggtgg atcctggatc
     1261 agtaatctgg cagatggtat tcggaacaag cttccggaga tcacgaagaa ggctggagaa
     1321 ctgattgtag ggttcctcct aggtctgagg cagaaagcag ccgacatagc tgaagcaggc
     1381 gttgctctca tcgtatccat catcaacggg atctcacgga agatcggcga tctcgtaact
     1441 gccgcataca acttggtcat caagtttgct gagggcgttc gcaagaacgt caacaagctc
     1501 atcgatgcgg gtatcgacct gatagcggac ttcttgcatg ccttggcgga ttcgattcgc
     1561 aatggcagtg ccgcaatcgg atccggtatt cgggatgtag ttgatgcatt cggtgacgtc
     1621 ggccgtgaca tgatcggtgg actgatcgac ggcattgaaa gcatgttcga tgatgtggtt
     1681 ggtgctgtca cgagtgtggt aggcgaactg ccgggatggg ccaagaaggt cctag
//
LOCUS       DASTWE010000276         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_718436, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000276 DASTWE010000000
VERSION     DASTWE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718436"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFT30_02970"
     CDS             complement(<1..243)
                     /locus_tag="VFT30_02970"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091615.1"
                     /translation="MKTPAKKSNDRRKTTKSNGVKYPDILRDLEGQRASILAEAGVVL
                     TSPTGLEIFPDVSDQASAEADQHFSFRIRERERKLLK"
     gene            complement(335..937)
                     /gene="recR"
                     /locus_tag="VFT30_02975"
     CDS             complement(335..937)
                     /gene="recR"
                     /locus_tag="VFT30_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU5091616.1"
                     /translation="MSIDQQGLLAKLVRELVRLPGIGRKSAQRLAFHILKSEREDALR
                     LAEAIQAVKDGLSFCRQCRNIAEEELCEFCRDPKRDRTTILVVEEPSTLYAIERAGGY
                     RGVYHVLLGVLSPLDGIGPSDIRAEELLDRVKGGGVEEVIVATNPTIEGEATAIYLTR
                     LLKPHHVRVSRIAYGIPVGMDIEYADDVTLVKSIEGRRDL"
     gene            complement(1016..1132)
                     /locus_tag="VFT30_02980"
     CDS             complement(1016..1132)
                     /locus_tag="VFT30_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5091617.1"
                     /translation="MLQDLVMAAGNDALKKSRDMMAEEMKSVTGGMKIPGLF"
BASE COUNT          250 a          339 c          274 g          275 t
ORIGIN      
        1 cttcaggagc tttcgctcgc gctcccgaat ccgaaaagaa aagtgttgat ctgcttcagc
       61 ggaggcttgg tcacttacgt ctggaaagat ttccaggccg gtgggacttg tcagcaccac
      121 tccggcttcc gccagaatcg aggcgcgctg gccttccagg tctcgtagaa tatcggggta
      181 cttgacccca ttcgatttag tggtcttccg tcgatcattg gatttttttg ccggagtctt
      241 catccttatt ttcgttcgat gctgtgctcg cagtgcccgc gtgggaatcg actatatcag
      301 tctccatcga aagcggtcaa ttggcctgtt accgtcaaag gtcccgtcgt ccctcgattg
      361 atttcactaa ggttacgtcg tcagcatatt caatatccat tccgacagga attccatagg
      421 caatgcgcga aaccctcaca tgatgaggct tcaacagtcg ggtcagatag atcgccgtcg
      481 cctcaccttc gatcgtagga ttcgtggcga cgatgacttc ctccactcct ccccccttga
      541 cccgatcgag caattcctcg gcacggatat cggaagggcc gataccgtcc agcggagaca
      601 aaacccccag caggacgtga tagacgcccc ggtatcctcc cgcgcgttcg atggcataga
      661 gtgtgcttgg ctcttcaacc accaagatcg tggtccgatc ccgtttggga tctcggcaga
      721 actcacatag ctcttcttcc gcaatattgc ggcattgacg gcaaaacgac aatccatctt
      781 tcacggcctg aatggcttcg gccaatcgca gcgcatcctc acgttcagac ttgaggatat
      841 gaaaggccaa tcgttgcgcg ctcttccggc cgattccagg caagcggacc aattcccgca
      901 ccagttttgc cagtaatccc tgttgatcga tgctcatgta agtgagtgca tccgcaggcg
      961 aaacaaaaga aaatgtgata gggcttcagg cgccatccaa ccagtcacca agccttcaaa
     1021 aaaggcccgg aatcttcatg ccacctgtca ctgacttcat ctcctccgcc atcatgtcgc
     1081 gggacttctt gagagcgtca ttgcctgcag ccatcaccag gtcttgcagc atgtcaac
//
LOCUS       DASTWE010000277         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1429460, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000277 DASTWE010000000
VERSION     DASTWE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429460"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1159
                     /locus_tag="VFT30_02985"
     CDS             <1..>1159
                     /locus_tag="VFT30_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091618.1"
                     /translation="GMFGTFVIEPKGSEYLDPLGTGEPTRMTSGWQAIIKNGAGPDFR
                     EFVLIYHEVGDEAFRPLNKKGDFIPQRDPLTDVYRPVARALNYRSEPFGVDNMQTQHE
                     YFGFEDESMAYSAYTFGDPATTVPRSYLGDPAKFRLVHGGSEVFHSHHPHGGSIRWPR
                     SPRAIDEMPLWHAAKNGPVKYPVIRMKSDRVDVEVIGPSETMDLETECGSGLCQQLAG
                     DFLFHCHVAHHYIAGMWGYWRVYNTLQQGEYHNDVMPDLRELPDRTGRIQTGITSDQL
                     IGRTVDWFGKTFTIVKDGKTNWKASPAIVNVKDWVNMMLPPQGQPGHQKDERAQTRSY
                     DASVLDWAWQGDRAMTEPENSVDNPRYKSTTPGKRVPILFESATGKVAWPH"
BASE COUNT          267 a          312 c          347 g          233 t
ORIGIN      
        1 tgggcatgtt cggcacgttc gtcatcgaac ccaaggggtc tgaatatctt gatccattgg
       61 gaaccggtga acccacacgg atgaccagcg gatggcaggc aatcatcaag aacggggctg
      121 ggccagactt ccgggaattc gtattgatct atcatgaagt cggggacgag gcgttccggc
      181 cgctgaacaa gaaaggcgac ttcattcctc agcgcgatcc cttgaccgac gtctatcggc
      241 cggtagcccg tgcattgaat tatcgtagcg agcccttcgg cgtcgataac atgcagacgc
      301 aacacgaata cttcggcttc gaagacgaat ctatggccta cagcgcttat accttcggcg
      361 atccggcaac aaccgtgccg cggagctatt taggagatcc ggcaaagttt cgcctcgtgc
      421 acggagggtc ggaagtgttt cactcgcacc atccgcacgg agggtcgatt cgttggccgc
      481 ggagtccgcg tgctatcgac gaaatgccct tatggcatgc ggctaagaat gggccggtca
      541 aatatcccgt gattcggatg aagtccgacc gcgtggatgt ggaagtcatc ggtccgtccg
      601 agaccatgga cttggaaacc gaatgcggat caggtctctg ccagcagttg gcgggtgact
      661 tcctgttcca ttgtcacgtc gctcaccatt acatcgcggg gatgtgggga tattggcggg
      721 tgtacaacac gctgcaacag ggagagtatc acaacgatgt gatgccggat ttgcgtgagc
      781 ttccggaccg gactggccgt attcaaacgg gtattacctc ggatcagctt atcggccgca
      841 ccgtggactg gttcggaaag acgttcacca tcgtcaaaga cggaaagaca aactggaagg
      901 cgtcacctgc catcgtcaat gtgaaggatt gggtcaacat gatgctgccg ccgcagggac
      961 agcctgggca tcagaaagat gagcgcgccc agacccgatc ctacgacgca agcgtgctag
     1021 attgggcctg gcagggtgat cgtgcgatga ccgaacccga aaacagcgtg gacaatccaa
     1081 ggtacaagtc aacgactccc ggcaagcggg tgccgattct ctttgaaagc gccaccggca
     1141 aggtcgcctg gccgcattt
//
LOCUS       DASTWE010000278         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_362819, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000278 DASTWE010000000
VERSION     DASTWE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362819"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="VFT30_02990"
     CDS             complement(<1..1619)
                     /locus_tag="VFT30_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEU5091619.1"
                     /translation="MLIEQADNARLSRRLATIQTDCPVEFDPDALQVKSAHNDRLMEL
                     LRELEFTTLLKSIQPTSPQASIHQASVETIESEAAAHAFVARLRKSETLAVQCLLSGP
                     PGVRADVQGIALSSEDKTAFVPLDVHAYMRPITALFHETTRLKAVHDLKLTLLAFHRI
                     GVTLAGPYFDTMIADYLLNPNRRDHQLDTLALELLDHRLGAQAKEPSPPTSLFQIETA
                     SQEQTAERAAVVAKLTLVMLDRLRDQGSLKLFQEVEMPLVSVLADIERNGFLLDVQGL
                     QALSKELEQDLDRMMTSIAVLAGGEFNINSPKQLATVLFEKLGLKPVRKTKTGYSTDE
                     DTLTQLAAQHELPAQILNYRSVSKLKSTYVDALPTLVHPDTKRLHTSLNQTVAATGRL
                     SSTDPNLQNIPVKGDYGLRIREAFIVPPGHELLCADYSQIEPRILAHLSQDPRLLSVF
                     TKEEDIHMATAMEIFSLPSGQITRDMRRAAKTVVFGIVYGISPFGLSQNLGVPQAEAK
                     KYIETFFERFPAVRALMDRNIAEGREKGYTTT"
BASE COUNT          322 a          424 c          506 g          390 t
ORIGIN      
        1 gtggtggtgt agcctttctc ccgtccttcg gcgatgttcc gatccatcag ggcacggacg
       61 gctgggaatc gttcaaagaa ggtttcgatg tatttttttg cctcagcctg aggtacgccg
      121 agattctgag aaagaccgaa cggactgatt ccatagacga tgccgaaaac gacggttttg
      181 gcggcgcggc gcatgtccct tgtaatctgg cccgacggca ggctgaagat ttccatggcc
      241 gttgccatat ggatgtcctc ttccttggta aagacggaca gcaaccgcgg atcttgcgaa
      301 agatgggcga ggatacgtgg ctcgatctgg ctatagtcag cgcagaggag ttcatggcct
      361 ggggggacga taaaggcttc gcggatgcgg agaccataat cgcctttcac gggaatattt
      421 tgaagattcg gatcggtgga cgagagccgt cctgtcgccg caacggtctg attcagcgac
      481 gtatggagcc gtttcgtgtc cggatgtacc aatgtgggca gcgcgtccac ataagtcgac
      541 ttgagcttgc ttacgcttcg gtagttcaaa atctgcgcag gcaactcgtg ttgcgctgca
      601 agttgcgtga gcgtgtcttc atcggttgaa tagccggtct ttgttttacg aaccggcttc
      661 agaccgagct tctcaaacag gactgtcgcg agttgctttg gcgaattgat gttgaattcg
      721 ccgccggcca ataccgcaat gctggtcatc atccggtcaa gatcctgctc caattccttg
      781 ctcaaggctt gaagcccttg gacatccagc aaaaacccgt tccgctcgat atcggctaag
      841 acggacacga gcggcatttc gacttcctgg aacagtttca gacttccctg atctcgcagc
      901 cgatcgagca tcaccagcgt gagcttcgcg accaccgccg cacgttccgc ggtttgctcc
      961 tgcgacgcgg tttcgatttg gaacaacgat gtcggcgggg atggttcttt cgcctgcgca
     1021 ccgagccgat ggtcgaggag ttcaagagct agggtgtcga gttgatggtc ccgtcgattg
     1081 ggatttagaa ggtagtctgc gatcatcgtg tcaaaatatg gtccggcgag cgtgacgccg
     1141 atccggtgaa acgcgaggag tgtcagtttc aagtcatgca cggccttgag ccgggtggtc
     1201 tcgtgaaaga gggcggtgat cggccgcatg tatgcatgca catcgagcgg gacaaaggca
     1261 gttttgtcct cagacgacag cgcgatgccc tgtacgtcgg ctcgtacgcc gggtggacct
     1321 gagagaagac attggacggc gagggtctcg ctctttctca atcgtgcgac aaaggcgtgc
     1381 gccgccgcct cgctctcgat cgtttccact gaagcttgat ggatcgaggc ctgtggcgag
     1441 gtgggctgga tggatttcaa tagtgtcgtg aattccagct cgcggagcag ctccatgagt
     1501 cggtcattgt gtgccgactt gacctgcagc gcatcggggt cgaattccac gggacagtcg
     1561 gtttgtatcg tggccagacg ccgactcagc ctggcgttgt cggcctgctc gatcaacatg
     1621 gttttggtcc tggccggggt ca
//
LOCUS       DASTWE010000279         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_298266, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000279 DASTWE010000000
VERSION     DASTWE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298266"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /gene="carA"
                     /locus_tag="VFT30_02995"
     CDS             <1..1100
                     /gene="carA"
                     /locus_tag="VFT30_02995"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5091620.1"
                     /translation="VGEVVFNTAMTGYQEILTDPSYKGQIITMTCPHIGNYGVTPEDV
                     ESRRAWAEGFVVMEASRLVSNWRSKQTLQESLKEAKVVAIEGIDTRALTRHLREHGSQ
                     QGLITHLNLDSDRAVGQARQAPSIIGRDLAAEVTCARPYDWTTGTGDWIPSANSNKRG
                     TFKPWRVVAYDFGIKHNILRRLVDVGCQVTVVPAATSAVEVETLKPDGIFLSNGPGDP
                     EGLPYAAKAMEKLIGRYPIFGICLGHQILCLAFGLKTYKLKFGHHGANHPVMDLRTRK
                     VEITSQNHNFAVQGPPPLLEVPKFPPVIDTRYGKLSLTHVSLNDYSIEGIACLDHPVF
                     SVQYHPEAAPGPHDAAYLFDEFVHLMETYHA"
     gene            1093..>1594
                     /locus_tag="VFT30_03000"
     CDS             1093..>1594
                     /locus_tag="VFT30_03000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEU5091621.1"
                     /translation="MHNRFVIGVIGLVLSGCTFNFPLFPGPGPLQETQVDGTGKAKVL
                     LIEISGVISSQDKEGFRTTPSMIASVKEQLTRAAKDENVKAVVLRINTPGGTVTASDI
                     IYHELKTFKASRKVPIVASIMDLGTSGGYYIAAAADTVLAHPSSVTGSIGVIMLTINA
                     RGLLEKV"
BASE COUNT          374 a          417 c          445 g          358 t
ORIGIN      
        1 cggttgggga ggtcgtcttt aataccgcca tgacgggcta tcaggagata ctgaccgatc
       61 catcctataa gggacagatc attacaatga cctgtcccca tatcggtaac tatggggtta
      121 cgcccgaaga tgttgaatcc cgtcgagcct gggcagaagg gttcgtggtc atggaagcca
      181 gccgccttgt cagtaactgg cggagcaagc agacccttca ggaatcatta aaggaggcaa
      241 aggtcgtcgc aatcgaaggg atcgatacga gagccttaac gaggcatctg cgcgagcatg
      301 ggtcacagca gggactcatc acgcacctca atcttgactc ggatcgtgct gtagggcaag
      361 ccaggcaagc tcccagcatc atcggtcgag atctggctgc cgaggtcacc tgcgcacgtc
      421 catatgactg gacgacgggg accggcgatt ggataccctc ggcaaacagc aataaacgtg
      481 gtacgttcaa gccctggcga gtcgtggcct atgacttcgg catcaaacac aatatcctga
      541 gacgattggt ggatgtcggc tgtcaggtga cggtggtgcc tgctgcgaca tcagccgttg
      601 aagtcgagac actcaagccc gacggcattt tcctctcaaa cggtcccggc gatccggagg
      661 gtcttcccta tgccgcgaag gcaatggaga aattgatcgg acgctatccg atttttggca
      721 tttgtttggg ccatcagatt ctctgcttgg ccttcggcct caagacctat aaactgaagt
      781 tcggtcacca tggggcaaat catcccgtga tggatcttcg aacgagaaaa gttgaaatta
      841 catcgcagaa ccataatttc gccgtgcagg gacccccgcc actccttgaa gtgccgaagt
      901 tcccgccggt gatcgacact cgctatggaa aactgtcgct cacccatgtc agcttgaatg
      961 actactcaat cgaggggata gcctgtctgg atcatccggt cttctccgtc cagtatcacc
     1021 cggaggccgc gccgggtccc catgacgctg cctacttgtt tgatgaattc gtgcatctta
     1081 tggagaccta tcatgcataa tcgtttcgtc attggggtga tcggattggt gttgtccggc
     1141 tgcacgttca attttccgct gtttcccggc cctgggcctc tgcaagaaac tcaggtcgac
     1201 gggaccggca aagcaaaagt gctgctgatt gaaatttccg gagtgatcag ttctcaagat
     1261 aaagagggat tccggacgac tcccagtatg attgcgagtg tgaaggaaca actgacccgg
     1321 gctgcaaagg atgagaatgt caaagctgtg gtgctccgca tcaatacgcc ggggggaacg
     1381 gtgacggcgt cggatattat ttatcacgag ctaaaaacat tcaaagccag tcgcaaggtc
     1441 cccatagttg catccatcat ggatctgggc acatcagggg gctactatat tgctgcggcg
     1501 gcggatacgg tactggcgca cccttcgtcc gttaccggca gtatcggcgt gatcatgctc
     1561 accatcaatg cgcgaggact cttagagaaa gttg
//
LOCUS       DASTWE010000280         4144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_169244, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000280 DASTWE010000000
VERSION     DASTWE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169244"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFT30_03005"
     CDS             <1..224
                     /locus_tag="VFT30_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091622.1"
                     /translation="LGSGRAQQVLAEIEMAQTQILFSDPTRVDENMAEVERRFEVFAR
                     RHRTSVRTLEHPRLSALQSVYSDIARGRL"
     gene            308..646
                     /locus_tag="VFT30_03010"
     CDS             308..646
                     /locus_tag="VFT30_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091623.1"
                     /translation="MPSLIEFPKFLRDPGEELVWSWKITGRDPYTISFGPEEVAPEVT
                     VVSTDWGSEEPYMTLWKAGPQLEMSMVSWPMVDLPHRVRAMFAMEFTCARGEMHEGDC
                     VPTNITLGEE"
     gene            643..1047
                     /locus_tag="VFT30_03015"
     CDS             643..1047
                     /locus_tag="VFT30_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091624.1"
                     /translation="MSQRPIIDSYAELVADAWLKMSGFRDALRRRGWVHMDDELTAAP
                     LAPIQRAILDAGVFGVGVITRGDDGSITNLDPKESVVFSIAEVEEVLRQVTSGEWTDQ
                     FALKYLYDPLVHALVSILVENGLIPSPKTNDG"
     gene            1292..1684
                     /locus_tag="VFT30_03020"
     CDS             1292..1684
                     /locus_tag="VFT30_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091625.1"
                     /translation="MDSKPPEGTRVVYVGEPRDDIKEIFGTVVLDAKDARSRSVVAFD
                     DGVQMSIHISDLTTQVQYEADQHPLSGLGSTEEIMEGITVSESEEVDPFAFRIWLIEN
                     GMVTEEQCDLDWINKLFGEFRIFLKEDE"
     gene            1684..2265
                     /locus_tag="VFT30_03025"
     CDS             1684..2265
                     /locus_tag="VFT30_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091626.1"
                     /translation="MDLEGVEEFAALRADQRDTRLAEFLNRQQRDGHSPQMVYFAMTI
                     EFTNLDEVKEHDCMACMRGGDLLLAVARHVRDGEWEPANTPLDAARELMVSVGTSMAT
                     EHHARDTWRRLYGQFKDLLPEVKFTPEVGDRVTCLAVNYFGSAAIVTEIFPAPPANPD
                     RRLIYGLSILDQDGVAYAVAHTSNVLPPIAPVE"
     gene            2271..2663
                     /locus_tag="VFT30_03030"
     CDS             2271..2663
                     /locus_tag="VFT30_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091627.1"
                     /translation="MNITMPFWAWSLFIIAIFLVAMMFLFMVDRYVKIYAELGVLDHQ
                     NTVLKDGPTCYVWIGDMRVGEMFFRFNEIPASRRHFMVDELPMSLNIERFGDGPEVLY
                     LLGPQHGRNPEAIRLFFGLDNPRKMVIP"
     gene            2660..2908
                     /locus_tag="VFT30_03035"
     CDS             2660..2908
                     /locus_tag="VFT30_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091628.1"
                     /translation="MKWLLFFPHDGSYAGEVNSEIGIKRKAAELILKDEKRYKQITLE
                     PEGEGTMIASWGGTEVLVAQTERHPDVTWVAASTRRKK"
     gene            2905..3237
                     /locus_tag="VFT30_03040"
     CDS             2905..3237
                     /locus_tag="VFT30_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091629.1"
                     /translation="MTGFSRIIDRWKSSQGSADDLRNMLIEAGWTPPIEKPERLDVPI
                     EKMRSRERHHDRIARADDDYASQAGDRHEEEIFRNKARGRRAKAAKYEYAADLLEVLR
                     QAGLRARS"
     gene            3234..3455
                     /locus_tag="VFT30_03045"
     CDS             3234..3455
                     /locus_tag="VFT30_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091630.1"
                     /translation="MSKRDEEIMRGNLRVAEAVVSLRTVTADIKVKKKAQRRLIRRLR
                     KAGLGTRFLAKVSDMSPQTILNISNSDEP"
     gene            3458..3814
                     /locus_tag="VFT30_03050"
     CDS             3458..3814
                     /locus_tag="VFT30_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091631.1"
                     /translation="MRSWMRRIGNARKIKNSKKSLAYQIMRLFEEAGEMSIEMRKADN
                     PEDLTVAGITFKKDKYGIDKPHGFAAEWGDTMNQLLHIADRVGIDPDAAMEIVTSYHE
                     TKRLVKPKKTKKKVKK"
     gene            3811..>4144
                     /locus_tag="VFT30_03055"
     CDS             3811..>4144
                     /locus_tag="VFT30_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091632.1"
                     /translation="MKTLPQPGSNIDTPSGTPGSVMRSTWVDEESKVGVLLSCHPARS
                     EMVYMVVCFAMYEGASKWLFDDGWWDEARMTNDLAEAINWYHEPLTLVLPAPQTPAGE
                     RLKKFFDDT"
BASE COUNT          906 a         1142 c         1305 g          791 t
ORIGIN      
        1 ggctcgggag cggtcgagca caacaggtgc tggctgagat cgagatggca cagacgcaga
       61 ttttgttctc cgacccgact cgggtggacg agaacatggc cgaggtcgag cgccggttcg
      121 aggtcttcgc tcgccggcac aggacgtccg tgcggaccct cgaacaccct cgtctctcgg
      181 ctttgcagtc cgtgtactcc gacatcgcga gggggcggct gtgagagtca tgagagcgct
      241 acgcaggtac ctgcttcgga actggcgcag gtggggtcgt gggatctacg ccaccatcca
      301 cttgcgcatg ccgagtctca ttgagttccc gaagttcctt cgggatcctg gtgaagaact
      361 tgtgtggtcg tggaagatca ccggacgtga tccgtacacc atctcgttcg ggccggaaga
      421 ggtcgctccg gaagtaacag tcgtgagtac agactggggc tcggaagagc cgtacatgac
      481 cctgtggaag gcagggcctc aacttgagat gagcatggtg tcatggccca tggtcgacct
      541 gcctcacagg gttcgggcca tgttcgcgat ggagttcacc tgcgcacgtg gcgagatgca
      601 cgaaggcgac tgcgtgccta ccaacatcac cctgggggaa gagtgagcca acgccccatc
      661 atcgattcct atgcagaact cgtggcggat gcctggctga agatgtcggg gttccgagac
      721 gcactgcgca gacgtggctg ggtccacatg gacgacgagc tcaccgccgc tcctctggca
      781 cccattcagc gtgcgatcct cgacgctggg gtcttcgggg tgggggtcat cacgagaggt
      841 gatgacggct cgatcaccaa cctggatccg aaggagtcgg tggtcttctc gatcgctgaa
      901 gtggaagaag ttctgaggca ggtcacgtcc ggtgagtgga cggatcagtt cgctctgaag
      961 tacctctacg acccgctcgt ccacgctctc gtgtcgattc tggtggagaa cggtctgatc
     1021 ccgagtccga agacgaacga tggttgagcg cacctacttc ttcgagatca aggcggatcc
     1081 ctcgaagctg gtcaaggaga tcaacaggac acgagacagg ctggggaagg ctcgggctac
     1141 gcacttgcgt gtcgtgcgtg ctgaagacga gtaccgaaga gctctcaaca aggacgagaa
     1201 ccggcgtcga gcccactggc gcaagcacgc tggcagacct cgttcacgca accgtcgcgg
     1261 ccgtcgtcgt cgctgaatcc gaaagggaca gatggacagc aagccaccgg aaggcaccag
     1321 ggtcgtctac gtgggagaac cccgagacga catcaaggag atcttcggaa ccgtcgtgct
     1381 cgatgcgaag gatgcgcgga gcagatccgt ggtggccttc gatgatgggg tgcagatgtc
     1441 gatccacatc tcggacctga cgacccaagt ccagtacgaa gccgatcagc atcctctctc
     1501 ggggttgggt tcgacagaag agatcatgga aggaatcacg gtctcggaat ccgaagaggt
     1561 tgaccccttc gcctttcgga tctggctcat cgagaacggg atggtcaccg aggagcagtg
     1621 cgatctcgac tggatcaaca agctcttcgg tgagttcagg atcttcctca aggaggacga
     1681 gtgatggatc tcgaaggcgt cgaggagttc gccgctctac gagcggatca gcgggacact
     1741 cgtctggctg agttcttgaa ccgtcagcag cgagatggtc actcgcccca gatggtgtac
     1801 ttcgccatga ctatcgagtt caccaacctc gatgaggtga aggagcatga ctgcatggcg
     1861 tgcatgcgag gtggagatct tcttctggcc gtggcacgac acgtgcgaga cggggagtgg
     1921 gagccggcca acacgccgct ggacgctgca cgtgagctca tggtgagcgt gggaacgtcc
     1981 atggcaacag agcatcacgc tcgcgacacg tggcgacgcc tgtacgggca gttcaaggac
     2041 cttctgccgg aagtcaagtt cacaccagag gtgggagatc gtgtgacctg cctcgctgtc
     2101 aactacttcg gatcagcggc gatcgtcacg gagatcttcc ccgctcctcc tgccaatccg
     2161 gaccgtcgtc tgatctacgg actcagcatc ctcgatcaag atggtgtggc ctacgccgtc
     2221 gctcacacct ccaacgtcct gcccccgatc gcacctgtcg aatgagccac atgaacatca
     2281 cgatgccctt ctgggcctgg tccctcttca tcatcgcgat cttcttggtc gcgatgatgt
     2341 tcctcttcat ggtggatcgc tatgtgaaga tttatgcgga gctcggggtg ctcgatcacc
     2401 agaacacggt tctgaaggac ggacctacct gctacgtctg gatcggagat atgagggtgg
     2461 gtgagatgtt cttcaggttc aacgagatcc ccgcctcccg tcgccacttc atggtcgatg
     2521 agcttccgat gtcgttgaac atcgagagat ttggagatgg tcccgaagtt ctctatctgc
     2581 tgggtcctca gcacggcagg aacccggagg ccatccgact cttcttcgga ctcgacaacc
     2641 cgaggaagat ggtgatcccg tgaaatggct tctcttcttc ccacatgacg ggtcgtacgc
     2701 aggcgaggtg aactccgaga tcggcatcaa gcgcaaggct gctgagctga tcctgaagga
     2761 cgagaagcgc tacaagcaga tcaccctaga gccagagggt gaggggacca tgattgcctc
     2821 ctggggaggg actgaagttc tcgttgctca gacggagcga caccctgacg tcacgtgggt
     2881 ggctgcttcg acgcggagga agaagtgacg gggttcagca ggatcatcga ccgatggaag
     2941 tcctcacaag gatccgccga tgatcttcgg aacatgttga tcgaggcggg ttggacaccc
     3001 cccatcgaaa agccagaacg actcgatgta ccgatcgaga agatgcgctc acgtgaacgt
     3061 catcatgatc gcatcgcacg tgcagatgat gactacgcct cgcaagcggg ggatcgacac
     3121 gaggaagaga tctttcggaa caaggcccga ggtcgtcgtg ccaaggcggc gaagtacgag
     3181 tacgcagcag atctgctgga agtcctgcgt caagccggac tgcgggcccg gtcatgagca
     3241 agcgtgacga agagatcatg cgaggcaacc tacgagtggc cgaagccgtg gtgagcctgc
     3301 gtactgtcac tgccgacatc aaggtgaaga agaaggctca gagacgattg atccgaagac
     3361 ttcggaaggc tggactcggc acccggttct tggctaaagt ttcggatatg agcccacaga
     3421 cgattctcaa catctcgaac tcggacgagc catgaacatg cgatcgtgga tgcggcggat
     3481 cgggaacgct cgcaagatca agaactcgaa gaaatcgctg gcgtaccaga tcatgcgtct
     3541 cttcgaggag gcgggcgaga tgtcgatcga gatgcggaag gcagacaacc ccgaagacct
     3601 caccgtcgcc ggcatcacgt tcaagaagga caagtatggc atcgacaagc cccatggctt
     3661 cgctgccgag tggggcgaca ccatgaacca actcctgcac atcgcggatc gggtgggcat
     3721 cgatccggat gctgcgatgg agatcgtgac ctcctaccac gagaccaagc gcctcgtgaa
     3781 gccgaagaag accaagaaga aggtgaagaa gtgaagacgc ttcctcaacc aggatcgaac
     3841 atcgacaccc cttcggggac tccggggagc gtgatgcgtt caacgtgggt ggacgaggag
     3901 agcaaggtgg gagttctcct ttcctgccac ccggctcggt ccgagatggt ctacatggtg
     3961 gtctgcttcg ccatgtacga aggtgccagc aagtggctct tcgatgacgg gtggtgggat
     4021 gaggcgagga tgacgaacga tctcgctgag gcgatcaact ggtaccacga gcctctgacg
     4081 ctcgttctgc ctgcccctca gacgccggca ggtgagcgtc tgaagaagtt cttcgatgac
     4141 acca
//
LOCUS       DASTWE010000281         1393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1332590, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000281 DASTWE010000000
VERSION     DASTWE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332590"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1393)
                     /locus_tag="VFT30_03060"
     CDS             complement(<1..>1393)
                     /locus_tag="VFT30_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5091633.1"
                     /translation="TKRGRDKPNISGFAEYGSFNTIREGAGLAGKKGPVDFSGSISRW
                     DTAGFSAINYRRGAAERDGYHNWQGSLRLGADLPKDGRIDFSFRWLNSLVNFDGFAFN
                     SVTFASDPADVLGAKSKDSQYIFAGNYMQPITSWWSQRLTLSRAMDNLVSDGGTVERN
                     VVTGAVGPIGFPFRSQIETTSNRIEWQHNVQIGKPLSLTAGYQFREQKGDNRNLLTNE
                     AVFADKIVSSHAGFGEAQLNLWDRVFGTAGIRQDEYNVFGSATTYRVTGGYLHHETGT
                     KLRGSYATGFRAPTINQLFFPGFGTPNLQPEKSQALDAAIEQTLPNDWGSISVGYFWT
                     RYRNLILSVFDPTVCMAPGSFGFCAQNAGLARAQGVEVNTKLKLYRDGRWVKSLDVQI
                     NYTYAATRDFSSGPDTRLPKWPLHQVTTIISYQPIEGLHANLEGRYFGQRFNNVGNDG
                     SVPSLFVWNLSASY"
BASE COUNT          327 a          395 c          332 g          339 t
ORIGIN      
        1 atagctcgca gacagattcc agacgaatag cgacgggacc gatccgtcat ttccaacgtt
       61 gttaaaccgc tggccgaagt agcgtccctc cagattggcg tgaagacctt caataggctg
      121 atagctgatg atcgtcgtga cttgatgaag cggccacttt ggaagacgtg tatccgggcc
      181 actactgaaa tcgcgcgtgg ccgcataggt atagttgatt tgtacatcta agctcttgac
      241 ccatcgtccg tcacgataca gtttcaactt cgtgttgact tccacgcctt gagctctggc
      301 caatcccgca ttctgagcac agaacccaaa tgaccccgga gccatacaga cggtgggatc
      361 gaacaccgac aagataaggt ttcgatatcg agtccaaaag taacccacgc tgatcgaacc
      421 ccaatcattg ggcaatgttt gttcgatggc cgcatctaag gcctggctct tctcaggctg
      481 caaattcggc gtcccgaagc ccggaaaaaa taattgattg atcgtcggag ctcgaaatcc
      541 ggtcgcataa ctgccgcgta gcttcgtgcc cgtttcgtga tgcaggtacc ctccggttac
      601 acgataggtt gtggcgcttc caaacacgtt gtactcgtct tgccgaatac cagccgttcc
      661 aaatacacga tcccaaaggt tcagctgagc ctctccaaat ccagcgtggc tgctgacgat
      721 tttgtccgca aataccgctt catttgtaag cagattacga ttgtcgcctt tctgttcacg
      781 aaattgatag cctgccgtca acgataatgg tttgccgatc tgcacattgt gctgccattc
      841 aatccggttg ctcgtagtct cgatttgaga tctgaaggga aacccgatcg gtccgaccgc
      901 gccggtcacg acgttgcgtt ctaccgttcc gccgtcactc acaagattgt ccatcgctcg
      961 tgacaacgtc agtcgttgtg accaccatga ggtgatcggc tgcatataat ttccggcaaa
     1021 gatgtattgg ctgtccttcg atttcgctcc caagacatcc gctggatcag atgcaaacgt
     1081 cactgaatta aacgcaaacc catcaaaatt cacgaggctg ttcaaccatc gaaaactaaa
     1141 atcgattctt ccgtccttgg gaaggtccgc gccgagccgg agcgatcctt gccaattgtg
     1201 gtagccatca cgttcagccg ccccacgtcg ataattgatt gccgaaaatc cggcagtgtc
     1261 ccaacgggag atcgatccgg aaaaatccac aggccccttt ttaccggcca ggccggctcc
     1321 ctctcgaatt gtattgaacg agccgtactc tgcaaatccg gagatgttgg gcttgtcccg
     1381 tccccgcttt gtc
//
LOCUS       DASTWE010000282         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_718570, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000282 DASTWE010000000
VERSION     DASTWE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718570"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFT30_03065"
     CDS             complement(<1..591)
                     /locus_tag="VFT30_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5091634.1"
                     /translation="MFKKILIANRGEIAMRIIRACRELNIATAAIYSEADSTGIYVKK
                     ADESYMVGPGPVKGFLDSKQIVDLAKRIGADAVHPGYGFLSENAEFAELCQASGITFI
                     GPSTQAITLMGSKVKAREIAESAGVPIVPGTDGAITSVKEALAFAKKAGYPVMIKASA
                     GGGGRGLRVVRSDDELRENMEVAAREAQASFGDGSVF"
     gene            894..1904
                     /locus_tag="VFT30_03070"
     CDS             894..1904
                     /locus_tag="VFT30_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091635.1"
                     /translation="MTTMGRWVKGGWAPLLAILWLVGFHSTPARSETPAPSVHWGSMS
                     FPDQYSTVTGGLTLNRFTPTDGLGNKYDSTVGNTLGFNLITLSWTQHGGGDWKNWSTN
                     LTAGIGPTGDEITNFLQNKFVHQLRQLPAVPTTNPRKETDAMIDGSVTRWFPLWGGPK
                     LLFAGGGFSVGTIYQQGFLRAGIRRLQITPTFYSGDWGKVSGRASVLGRVSYQDNGAV
                     LHSIRNTAALVQPSVAFGQYRTTENGETIPTWEIELALMWDSGIFVNTVGRSQEQFAW
                     AIAASAGPVRFETWNDSMGNIVERDYGPTYGATLTLDVLRTWNIIQCLRSKSSADAAE
                     KL"
     gene            complement(2119..2700)
                     /locus_tag="VFT30_03075"
     CDS             complement(2119..2700)
                     /locus_tag="VFT30_03075"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU5091636.1"
                     /translation="MIRKTVGRILGGLCAIMLAGVHADVAQAGETGGIPPELVAEYIH
                     AVIQADRSIYSTHVVEQLQDRKVTGAAEDWKEKGALPLPAQMLQMAGQAVQGLGLGLR
                     IRLSSLGPIYTKNGPADQFEREGLEAVAKNPRKPYTGIISEGDRRYFKAIFADRAVSM
                     ACVICHNSHSLSAKRDYKLHDVMGGIIISFPVR"
BASE COUNT          664 a          757 c          771 g          692 t
ORIGIN      
        1 gaagacgctg ccgtctccga aggacgcctg ggcttctcgc gcagcgactt ccatattctc
       61 tcgcagctca tcgtcggatc ggaccacgcg aagcccgcga ccgccaccgc cggcgctcgc
      121 cttgatcatg acggggtagc ctgctttttt tgcgaaggcc agggcttctt ttacactggt
      181 gatggctcca tccgtaccgg gcacgatcgg aacgcccgct gattcggcga tctcgcgcgc
      241 cttgaccttg ctacccatga gggtaatggc ctgggtggac ggaccaatga acgtaatgcc
      301 ggaagcctgg cagagctcag caaactccgc gttttcagaa agaaacccat acccgggatg
      361 gacggcatcg gcgccgatcc gtttggccaa gtctacgatc tgtttgctat ctaagaaacc
      421 cttcaccggt ccgggcccga ccatgtagga ttcgtccgct ttcttcacgt agatcccggt
      481 cgaatccgct tcggaataga tggcagcggt ggcgatgttc aactcgcggc aggcgcggat
      541 gatccgcatg gcgatctcac cgcgattggc gattagaatc ttcttgaaca tgtctgagcc
      601 tggcagcttt cgtgctgaaa ggaatgaatc taaaaacgaa cgcgtaagct agcataggac
      661 caggaggtct gtcgagggag aagcgctgac gagaagtcag aatcacacat caaaacgaag
      721 agttacatct tgtcggttaa taattaggtc gccgctttcg ccggtgttgt gtagaatgcc
      781 gccaattgaa agcggtgttt ggtgttttct atataggcaa atactaccct acgcctagga
      841 ttcggtccaa acagtcatgg cgatgggtac atgtcgatag gagatcacat tcaatgacaa
      901 caatgggtcg gtgggtgaag ggaggatggg ctcctcttct cgcgattctt tggcttgtag
      961 gatttcattc tactccggca aggagtgaaa ctcctgcacc atccgtccac tgggggagta
     1021 tgtcgttccc agaccagtac tccacagtta ccggagggct gactctcaat cggttcacgc
     1081 cgacggatgg actggggaat aagtatgatt ccacggtggg gaatacgctc ggcttcaact
     1141 tgatcacgct cagttggact caacatgggg gtggggattg gaaaaactgg agtaccaacc
     1201 ttacggctgg gatcggaccc acgggcgacg aaatcacgaa ttttcttcag aataaattcg
     1261 tgcatcaact tcgccaactt ccggcagttc cgacaacgaa ccctcgtaaa gagaccgacg
     1321 cgatgatcga tgggtctgtg acccgctggt ttcccttgtg gggtggacca aagctcctat
     1381 tcgcgggcgg tggcttttcc gtcgggacca tctatcaaca ggggtttctt cgagctggca
     1441 taaggcgctt acagattacc cccacattct attccggaga ctggggtaaa gtgagcgggc
     1501 gtgcatctgt cctggggcga gtcagttatc aggacaatgg agcagtgctg cattcaatca
     1561 ggaatacggc agctctggta cagccctctg tagcctttgg tcagtatagg actaccgaga
     1621 atggtgaaac aattccgaca tgggaaatcg aactcgccct tatgtgggat tctggaattt
     1681 ttgtcaatac agttgggagg agtcaggaac aatttgcctg ggccatagcg gcttcagccg
     1741 ggccggtgcg gttcgagaca tggaatgata gtatgggaaa tattgtcgag cgagactacg
     1801 gccctacgta cggcgcgacg ctgacactcg acgttctgag aacatggaac atcattcaat
     1861 gcctccgatc gaagtcctcc gctgatgcgg cggaaaaatt gtagaatccc ttactcctgt
     1921 gctaggaaag ttttgagctg gttgaatcct ctaaacgcgt cccctgaata aggcaatttt
     1981 gcacaaagtc gaaccaaccc tgagggctga tgaatgactg gtggctagtt ctgaaatctc
     2041 ggccacgtcg atgtcatgga aagtttaaag attcatatgg caagaaaaga cttcgggggt
     2101 gaggtcgatc gcagaggttc aacgaaccgg aaaagaaatg ataattcctc ccatcacatc
     2161 atggagcttg tagtcccgct tcgcactcag agagtgactg ttatggcata tgacacaagc
     2221 catggagacg gcccggtccg caaatattgc cttgaaataa cgtctgtcac cttctgagat
     2281 gattccggta tagggcttcc gtggattttt agccacggct tccaggcctt ctctttcaaa
     2341 ctgatccgcc ggcccgttct ttgtataaat tggccctagg ctgctcagcc gaatacggag
     2401 gccgaggccg agcccctgaa ctgcttgacc tgccatctgt aacatttgag ctggaagagg
     2461 caatgcccct ttctctttcc aatcttctgc cgccccagtg accttgcgat cctgcagttg
     2521 ctcgacgacg tgggtagaat agatactccg atcggcttga atgaccgcat gaatgtattc
     2581 tgccaccagt tccggtggga ttccgcctgt ctctcccgcc tgcgcaacat ctgcgtggac
     2641 accggcgagc atgatggcgc acaggccgcc aagaatcctt ccgactgttt tccgaatcat
     2701 ggcaacctcc ttcgtagctt ctcgaagcag aatctgcctc gaagagcgct tctaacgagg
     2761 tcattgcctc catctccgct actttgtcat cttttcaatt cttcgacgct gccaatgtcc
     2821 ttataactac tgaaatccga ttacttggat gattgcttcg catccaagat aaagatctcc
     2881 cctc
//
LOCUS       DASTWE010000283         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_880127, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000283 DASTWE010000000
VERSION     DASTWE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880127"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..>1013)
                     /locus_tag="VFT30_03080"
     CDS             complement(57..>1013)
                     /locus_tag="VFT30_03080"
                     /inference="COORDINATES: protein motif:HMM:NF023294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HEU5091637.1"
                     /translation="LRGVQKATALVHFFFFSMRSGPSFHAIPFAKSAAIPAPSPTGGE
                     KLSNYIRIQKDLKMPPGIGRILIPPLSGEKGCEYNESFASLLTTEGELIMNTHTSDQP
                     TVADDPKAREALRRAFENTARWPKDFKGFTAELTVNINGREFHGPVTVKGPREVSVQL
                     GEGEVQKWAQEQLAMIAVHRGPRTFEESDGKYTLTMEDDGHPLGTKLDIHGSHSFYRV
                     KDNRITQINRTMSHPGTAPFAFTINVEESAVTQDKKNLTTKYSVYYFSPEDGKLKNVE
                     SFTDSHVRVGASDLPATRRIIAFENGRVVVKSLTMTNHQLLS"
BASE COUNT          201 a          274 c          285 g          253 t
ORIGIN      
        1 tcatcaggtg gggagcgtga aggcggcggg tcaaaccgcc gcctcctcca gcgaaactac
       61 gacagcaatt gatggttcgt catcgtgagg cttttcacga cgaccctgcc gttctcaaac
      121 gcaatgatgc gccgggtcgc gggcaaatct gaagcgccca ctcgcacatg tgaatccgtg
      181 aagctctcga cattttttaa ttttccgtct tccggagaaa aataatagac cgagtatttt
      241 gtcgtgagat tcttcttgtc ctgtgtcacg gcgctttctt cgacattaat cgtaaaggca
      301 aagggcgccg tgccggggtg tgacatggtt ctattgattt gcgtgatgcg gttgtccttg
      361 acacggtaaa acgagtgaga gccgtgaata tccagtttgg ttcccaacgg atgaccatcg
      421 tcctccatcg tgagtgtata tttgccgtct gattcctcga acgtccgtgg cccccgatgg
      481 acggcgatca tggcaagctg ctcctgagcc catttctgga cttcaccctc gccgagttgg
      541 acggagacct cacgcggccc tttcacagtg acggggccgt ggaattccct gccattgatg
      601 ttgacggtga gctccgcggt gaatcctttg aagtccttcg gccaccgggc ggtattttcg
      661 aacgcccggc gaagcgcttc ccgcgctttc gggtcatcgg cgacggtcgg ctgatctgag
      721 gtgtgggtat tcatgatgag ttctccttcg gtggtcaaaa gtgacgcgaa gctctcatta
      781 tactcgcacc ctttttctcc gctcaatgga gggatgagga tccttccgat tcccggcggc
      841 attttcaaat ccttctggat tctgatatag ttcgacaatt tttctccccc cgtcggcgac
      901 ggggcgggga tcgcagcgga tttcgcaaag gggatggcat ggaacgacgg gccgcttctc
      961 atactgaaga agaagaaatg aaccagcgcc gtcgccttct gaacgccgcg aag
//
LOCUS       DASTWE010000284         1838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_234098, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000284 DASTWE010000000
VERSION     DASTWE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234098"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1167
                     /locus_tag="VFT30_03085"
     CDS             232..1167
                     /locus_tag="VFT30_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091638.1"
                     /translation="MNCTRCKTKAVLKLPRHNAAFCKDCFNNFVHDQVLKAIRSHEMF
                     KKTDRLLVAVSGGKDSLALWDILLKLGYKTDALYVNLGIGGYSESSQEKVVHFSETVA
                     AGYGAMLHVHTVEREEGAGIRELAMMVHRPTCSTCGTIKRYQFNHAAIRHGYDVMATG
                     HNLDDEAARLLGNVLHWQNEYLEKQGPSLPASVDGFAKKVKPLYRLTERELAAYCVLN
                     RIDYVVEECPMAQGARTLLYKEVLNRLETESPGTKQMFYWGFLEKSHTTETPTTIMEK
                     DRSSLHPCSLCGQPTTAETCSYCKLMAKAKTSTSR"
     gene            1245..>1838
                     /locus_tag="VFT30_03090"
     CDS             1245..>1838
                     /locus_tag="VFT30_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5091639.1"
                     /translation="MATTPRDYYHILGVPRTASPDDIKKAFRRLARQYHPDLHAGAKK
                     AEMEKKFKELNEAQEVLTDPEKRKKYDQYGADWDQAQAFEKAARQQARSQGFGGPWGA
                     EGDYTGQGSGGSGSDQFSDFFENLFGNRGRGGAGRSSSGMPGEDIETDVQLGMREVLT
                     GVTKRVNLREPRTCSTCLGSGTVRGRSCATCQGTGMTA"
BASE COUNT          442 a          494 c          485 g          417 t
ORIGIN      
        1 tgataagata tattatgtta actgtcagtt ttcttactcg accccctatt ccttatacct
       61 tcatagccga tcgtagttcg aaggtcaatc gtgcagcggt tttcttgagc ttcgtcaaat
      121 cttatgacac ccttgctctc gtcaacatcc catcatctat cgtccagcat cttgcttcct
      181 gctggcggta tccgtttgca atccccccgt tacgcccgta tgatcgcaac gatgaactgt
      241 acgcgatgta agaccaaagc cgttcttaag ttgcctcgcc ataatgccgc attctgcaag
      301 gactgcttta acaacttcgt gcatgaccag gttcttaaag cgattcgatc tcatgagatg
      361 ttcaagaaaa ccgatcgtct ccttgtcgcc gtttcaggtg gcaaggacag tttggccctc
      421 tgggatattc ttctgaagct gggctataag accgatgcgc tgtatgtgaa ccttgggatc
      481 ggaggttact ccgaatcatc acaagaaaag gtcgtgcatt tttccgagac cgtggccgcg
      541 ggatacggtg cgatgctcca tgtccatacg gtcgaacgag aagaaggcgc cggcattcga
      601 gagctggcta tgatggtcca ccgtccgact tgctcgacct gcggcacgat caaacgttat
      661 cagttcaacc atgctgcaat ccggcatggg tacgatgtca tggcgacggg acataatttg
      721 gatgatgaag cggctcgact gctcgggaac gtgctgcatt ggcagaacga atatcttgag
      781 aaacaaggtc ctagccttcc cgcctctgtc gatggattcg ccaagaaagt gaagcctctg
      841 tatcgcttga ctgagcgaga attggcggcc tattgcgtcc tgaataggat cgactatgtt
      901 gttgaggaat gtccaatggc gcaaggagcg cgcacactcc tctacaagga ggttttgaat
      961 cgcctagaaa ccgagtcgcc aggcaccaag cagatgtttt actgggggtt ccttgagaag
     1021 tcacacacga ccgagacacc cacgaccatt atggaaaaag atcgctcgtc cctgcatccc
     1081 tgttccctct gtggccagcc caccacggcc gagacctgct cctactgcaa actaatggcc
     1141 aaggcaaaaa cttcgacgtc gcgttgaccc attcagccct tgcaaaccgt ccctacactc
     1201 cgtaagctga tccgtaacat cacgtttctc atcgcatatc acgtatggca actaccccgc
     1261 gcgattatta tcacattctg ggcgtccccc gaaccgcctc gcccgacgat atcaaaaaag
     1321 cttttcgacg cctggcccgc cagtaccatc cagatctcca tgccggcgcg aagaaggccg
     1381 agatggaaaa gaaatttaaa gagctcaatg aggcacagga ggtcctgact gacccggaga
     1441 agcggaagaa gtatgaccag tacggagctg attgggatca agcacaggcc ttcgagaaag
     1501 ctgctcgtca gcaggccagg tcacaagggt tcggaggacc atggggggcc gaaggagact
     1561 acaccggtca aggcagtggc ggatccggaa gcgatcaatt ctccgatttt tttgagaacc
     1621 tgtttggaaa ccgcggacgc ggcggagccg gacgcagtag ttctggaatg ccgggagagg
     1681 acatcgagac cgatgtccag ttgggaatgc gtgaagtatt aactggtgtc acgaaacgcg
     1741 tgaatctgcg tgaacctcgg acgtgctcaa cttgtctagg gagcggtacc gtgcgtggcc
     1801 gatcttgcgc gacctgtcag ggaaccggga tgacggcc
//
LOCUS       DASTWE010000285         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1364987, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000285 DASTWE010000000
VERSION     DASTWE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364987"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /gene="fliP"
                     /locus_tag="VFT30_03095"
     CDS             complement(<1..704)
                     /gene="fliP"
                     /locus_tag="VFT30_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248836.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEU5091640.1"
                     /translation="MTDKYESRSRLLSLIIVGAMTLLVMPPSEAVAAGPSVSIDFGAD
                     GPKQTAVTIQILILLTVLSLAPALFIMVTSFTRIVIVLAFLRQALGTPTVPPNQVLLS
                     LALFLTMFIMAPVGQAVYTNALQPLMAEQISYEDAWKKGIEPIRNFMLRQVREKDLEL
                     FIKLSRMPKPDRVEDVPTQAIIPAFILSELRIAFQIGFLIYIPFLIVDMVVASILMSM
                     GMMLLPPVVISLPFKL"
     gene            complement(701..1087)
                     /locus_tag="VFT30_03100"
     CDS             complement(701..1087)
                     /locus_tag="VFT30_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEU5091641.1"
                     /translation="MIDLWESLFRTVSALAIVLVLMGIVAVTVRRLMGQRLGIAGGRP
                     LVRVLASSYIAPRKTIALVSVAGEYLIVGTTATDLVPLGRISDSSELRELLALASEKP
                     STEPAPLQRDTITSWLRRFSSRQGTS"
     gene            complement(1107..1475)
                     /gene="fliN"
                     /locus_tag="VFT30_03105"
     CDS             complement(1107..1475)
                     /gene="fliN"
                     /locus_tag="VFT30_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEU5091642.1"
                     /translation="MAEPDSATRAESQTTGSQSPQPASFPPVQQAETGGAPKNIDFIL
                     DIPMSVTVYVGSTKMAIRDLLQLAQGSVIELDKLAGEPMEVMVNNKLVARGEVVVVNE
                     KFGIRLTDVVSAAERVQQLR"
     gene            complement(1502..>2068)
                     /locus_tag="VFT30_03110"
     CDS             complement(1502..>2068)
                     /locus_tag="VFT30_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEU5091643.1"
                     /translation="QTHVKPEGRDFTAVQMRIIKKLLLLSLVDLEKAWHAVVPVKVEY
                     VRSESNPQFAMVVTSSEIVVVVTLQIILGETARELYIVYPYSMLEPIKEKLYSGLVSD
                     HVDQNGEWTHRFRERLQDCPLPIAVRLGTATVTVKDVMNFSPGDVILLDQHPGDPLDC
                     YIEGHHKFQGSPGIYKGNHACRVTKIVN"
BASE COUNT          477 a          584 c          558 g          449 t
ORIGIN      
        1 atcaacttga acggcagaga gatcacgaca ggtggaagga gcatcatgcc catcgacatc
       61 agaatgctgg cgacgaccat atcgacgata agaaaaggaa tgtagattaa gaatccaatt
      121 tggaatgcga tccgtagttc gctcagaatg aaggccggga tgatcgcctg ggtcggcacg
      181 tcctcgaccc ggtctggttt cggcatgcgg ctcaacttga taaagagttc gagatccttc
      241 tccctcacct gccggagcat aaaattccgg atcggctcga tgcccttctt ccatgcgtct
      301 tcataagaga tctgctcggc cattaatggt tgcagcgcgt tggtgtatac cgcctgaccc
      361 accggagcca tgatgaacat cgtcaaaaac agcgccaacg acagcaacac ttggtttgga
      421 ggaaccgtcg gcgttcccag cgcttgccgg aggaatgcca gcacgatcac gattctggta
      481 aaagacgtca ccatgatgaa taaggccggt gccaaggaga gcaccgtgag gagaattaaa
      541 atctggatcg ttacagcagt ctgtttcgga ccatctgcac caaaatcgat gctcaccgat
      601 gggccggccg ccaccgcttc tgaaggcggc atgacgagca gcgtcatcgc tccgacgata
      661 atcaacgaca ataggcgaga gcgagactcg tacttatcgg tcatgaagtc ccttgtcgtg
      721 atgaaaagcg tcgaagccag gaggtaatgg tgtcccgctg gagcggagcc ggttcagtgg
      781 agggtttctc gctggcgaga gcgagcaatt cgcgtaattc tgaggaatcg ctgatgcgtc
      841 ccaacgggac aagatcggtc gcggtcgtcc ccacgatcag atattctcca gcgaccgaca
      901 cgagggcaat cgtcttgcgc ggagcgatgt agctgctggc taagacccga acgagaggac
      961 gtccgccggc gatccccaag cgctgtccca tcaatcgccg gaccgtcacc gcaacgatcc
     1021 ccatcaatac cagcacgatg gccagggccg aaacggtgcg gaacaggctt tcccataaat
     1081 caatcacggc ctctcccttg gatcattcag cgaagttgct gcacgcgttc cgccgcacta
     1141 accacatccg tcaagcgaat gccgaatttt tcattgacga ccaccacttc accgcgcgca
     1201 accaatttgt tgttcaccat cacttccatc ggctcacccg ccagtttatc cagttcaatg
     1261 acggaacctt gagccagttg caacagatcc cgaatggcca tcttcgtgga tccgacatac
     1321 acggtgacgc tcatcggaat atccaggata aaatcgatgt tcttcggagc tcctcccgtc
     1381 tccgcctgtt ggacgggcgg aaacgaggcc ggttgcgggg attggctacc ggtcgtctgc
     1441 gattcggcac gagttgcgga atctggttct gccattggtc actctctcgg tggtgtctcc
     1501 actagttcac tattttggtc acgcgacacg cgtgattgcc tttgtaaatt cccggactac
     1561 cctggaattt gtgatgtccc tcgatgtagc aatcgagtgg atctcccggg tgctggtcga
     1621 gcaggatcac gtcacccggg gaaaaattca tgacgtcctt caccgtgacg gtggcggttc
     1681 ccagccggac cgctatcgga agtggacagt cttgtaaccg ttctcggaag cgatgggtcc
     1741 actcgccgtt ttgatcgacg tgatcagata cgaggccgga gtacagtttc tctttgatcg
     1801 gctcgagcat gctgtaggga tagacgatgt acaactcgcg ggccgtttct cccaagatga
     1861 tctgcaacgt gacgacgacc actatttcag atgaggtcac caccatcgcg aactgagggt
     1921 tgctctcaga acgcacatac tccaccttga ccggcacgac ggcatgccat gctttttcca
     1981 gatcgacaag cgatagcaac aacagcttct tgatgatcct catttggacg gccgtgaagt
     2041 ctcgcccctc gggcttcacg tgggtctg
//
LOCUS       DASTWE010000286         1250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_169433, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000286 DASTWE010000000
VERSION     DASTWE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169433"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFT30_03115"
     CDS             <1..755
                     /locus_tag="VFT30_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5091644.1"
                     /translation="IENIVPNRYQPRHTFSKEDLAELAASIKESGVLQPIMVRRKGDG
                     IYELIAGERRWRASKEAGLETIPVVIRNCSDQEALLLALVENLQREDLNPMETARAYS
                     RMMNEFGLTQDAIAVKVGRDRSSIANFVRLIHLHPEVQELVEGGTLTTGHAKALLGLA
                     SPEEQLRIGKMVAAGTLSVRETEKLVELNLVGKKRQPKSPRSSPWTDLEARLQKRLGT
                     KVTVHPHKQGGKVVIHYFSPDELDGVVEALLS"
     gene            916..>1250
                     /locus_tag="VFT30_03120"
     CDS             916..>1250
                     /locus_tag="VFT30_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5091645.1"
                     /translation="MWKDKQDGRRSDEVDIEGNGSSLEPMRHDSGQDVSAFVGKGVVF
                     KGTITYNGTVRIDGTLEGEIHTDGILLVGEEAVITAKVTAGTIVCKGKITGDIHARDK
                     MKLRAPAII"
BASE COUNT          335 a          323 c          326 g          266 t
ORIGIN      
        1 ggatcgagaa tatcgtgccc aatcgctatc aaccgcgaca caccttctcc aaggaagacc
       61 tcgctgagct ggcggcttcc atcaaagaga gcggagttct tcagccgatt atggttcgcc
      121 gcaagggcga cggcatctac gaattgatcg ccggagaacg ccgttggcgc gcgtcgaagg
      181 aagccggttt ggaaaccatt ccagtcgtta ttcgcaattg cagtgatcag gaagctctct
      241 tgctggcatt ggttgagaat ctccaacgag aagacttgaa tccaatggag actgcacggg
      301 cctattcgcg catgatgaat gaattcggcc ttacccaaga cgcgatcgcc gtaaaagtcg
      361 gtcgtgatcg ttcctccatc gcaaacttcg ttcgactgat ccaccttcac cctgaagtac
      421 aggaacttgt tgaaggagga actctgacaa cgggtcatgc gaaagccctg cttggcctcg
      481 catccccgga ggagcagctt agaatcggca aaatggtggc cgctggaact ctttctgtca
      541 gggaaacaga aaaactcgtg gagctcaacc ttgtaggaaa aaagcggcaa cccaagagtc
      601 cccgcagctc accctggact gatctcgaag ccaggttaca gaaacgcctt ggcacgaaag
      661 tgacggtcca tccacacaag caaggaggaa aagtcgtcat tcactatttt tcccctgacg
      721 aactcgatgg agttgtagaa gctctcctga gttagccttc atcttggttc gtgctcgccc
      781 aaaaatcgga tcttattgcc ttttaggcat atagacgcta aatatctcac cggaacagac
      841 cgatatgttc cctaacgata aagactccgc tcttcaatcg gacccctatc ccaaatcgtg
      901 agaggaggcg agcgtatgtg gaaggacaaa caagacggta ggcgttccga cgaggttgat
      961 attgaaggca atggatcgag cctggaacct atgcgtcacg actccgggca agatgtcagc
     1021 gcttttgtcg ggaaaggcgt cgtgttcaaa ggtaccatca cctacaacgg aactgtacgt
     1081 atcgacggga ctctcgaagg agaaatccac accgacggca tcctattggt cggcgaggaa
     1141 gctgtcatta cggccaaagt gacggccggc accattgtct gtaagggaaa gattacagga
     1201 gatattcacg ccagagacaa aatgaagtta cgtgctcctg cgattatcaa
//
LOCUS       DASTWE010000287         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_201711, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000287 DASTWE010000000
VERSION     DASTWE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201711"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..>1084)
                     /locus_tag="VFT30_03125"
     CDS             complement(415..>1084)
                     /locus_tag="VFT30_03125"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEU5091646.1"
                     /translation="VKAMAVRIRENLPARVDLDDLVHAGILGLFAAIENFDPDKRVPF
                     PSFAKYRIRGAMLDSLRKLDWASRNHRRRKREVAAATNELTMTMNRVPTESEVAEKLG
                     MDIDSWHKMILDFRNAEVVSATMGSEAESHQLDFRCKQETHPDSIYSHGELCSTLHVA
                     MEKLPARYRTVLRLHYDQDMTMRDIGVLMNVNESRICQIHKEALKKMRLQLETSGVES
                     AMAF"
BASE COUNT          236 a          287 c          284 g          277 t
ORIGIN      
        1 gaccactaag gaatgtttcg acgtgagcaa gttcggttga gcttcgccgg caagggaaga
       61 aagcctgctt ggacgtcgaa tcacttcaga gaactaggct tttagttggt ttattcctat
      121 ttcctttagt ctgcgcacga actgactcct ttgaggcatg gggcgacaga agagatgagg
      181 agagcattgt ggtgtgcgct gctgccaatt gatgtggctg cccagtgttg cagagaacgt
      241 aaaatgagcc cgcgcaccca gttttgggat ggtactgcgc aattgttcgt atataccgtg
      301 acaatgagca cgcacttcct gtcgttcaat ccatctattt atccagccgc agacgcatgg
      361 aacgggccgc aacagagttt cttctccact gatgaaagca gtaccaaccg gaggttagaa
      421 ggccatcgcg gattcaacgc cgcttgtttc cagctggaga cgcattttct tgagagcttc
      481 cttgtgaatt tggcaaatgc ggctttcatt gacgttcatc aggacgccaa tatctctcat
      541 cgtcatgtcc tggtcgtagt gcagcctcaa caccgtccgg tacctggccg gcaacttctc
      601 catggcgaca tggagcgttg agcatagctc cccgtggctg tagatcgaat cgggatgggt
      661 ctcttgcttg catcggaagt ccagttggtg actttccgct tcggacccca ttgtggccga
      721 aacgacctcc gcatttcgaa aatccaggat cattttgtgc cacgaatcga tgtccatgcc
      781 gagcttttca gcgacttccg attcagtagg cacgcggttc attgtcatcg taagctcgtt
      841 agtcgcagcc gccacctccc tcttgcgacg gcgatgattt cgcgaggccc aatctagttt
      901 ccgcaaacta tccaacatcg cgccgcggat ccggtacttg gcgaagctcg gaaaaggcac
      961 tctcttatct ggatcgaaat tttcaatggc ggcgaaaagt ccgaggatgc ctgcgtgaac
     1021 caggtcgtct agatcgactc tcgccggcag gttctcacgg attctaacgg ccattgcttt
     1081 taca
//
LOCUS       DASTWE010000288         1361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_104706, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000288 DASTWE010000000
VERSION     DASTWE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104706"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFT30_03130"
     CDS             <1..772
                     /locus_tag="VFT30_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5091647.1"
                     /translation="MAHAFTNHHYLLYSMATFGALMTSFYMFRLAYLTFYGASRMDSH
                     TAEHVHESPMVMVAPLVVLAFLSIVGGVILGFPPEHGWLHGFLGSVVGVGGKHETGAG
                     MVFLLMSIAIVIALMGWGLAHFLYAVSPVTADGWAEKFSGLYRILWNKYYVDELYDLI
                     FVEPLKRLGAILDWFDRMVIDGIVRGVGRLADWGSVGSTWIEKYIIYGGLNVIGYSNH
                     LAAREGRKMQSGMVHHYAAIIVAGLFLLVLVVQLFAQL"
     gene            798..>1361
                     /locus_tag="VFT30_03135"
     CDS             798..>1361
                     /locus_tag="VFT30_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5091648.1"
                     /translation="MLEELTAGFPILSCILFLPVVGAAVLWLFDEEDMIRTSALTIAL
                     VELALSVFVLLRFVPESAAMQFTERVRWIPALGISYHLAVDGISVLFVGLTAFLTVLV
                     VVYSWDTIRHQVKLYMMCLLALETTTMGVFVSLDLILFFVFWELMLIPSYFLIKLWGG
                     GAERHYAALKFVIYTLLGSVFMLVGIAL"
BASE COUNT          233 a          329 c          395 g          404 t
ORIGIN      
        1 tatggcccat gccttcacga atcatcacta tctcctgtat agcatggcga ccttcggggc
       61 actgatgaca tctttttata tgtttcgctt ggcctatttg acgttctacg gtgcttctcg
      121 gatggattct catacggctg aacatgtgca cgaatctccg atggtcatgg tcgccccctt
      181 ggtggtcctt gccttcctct cgatcgtggg gggggtgatc ttggggtttc ccccagagca
      241 tggctggctg catggatttt tggggtctgt cgtcggggtt gggggcaagc atgagaccgg
      301 tgccggcatg gtgtttctgt tgatgagcat tgcgatcgtc attgccttga tgggatgggg
      361 gctcgcacat ttcctctatg ccgtgagccc ggtcactgct gatggatggg ctgagaaatt
      421 ttccggtctg tatcgaattc tctggaacaa atactatgtc gacgaactat acgacctgat
      481 ctttgtggaa cccctcaaac gtctgggtgc gatccttgac tggttcgatc gcatggtcat
      541 tgacggtatc gtacggggcg ttgggagact cgctgattgg ggttctgtcg ggtctacgtg
      601 gatcgagaag tatattatct acgggggcct gaacgtgatc ggatacagta accacctcgc
      661 ggctcgtgaa ggtcggaaga tgcagagtgg aatggtccat cattatgccg ccatcatcgt
      721 tgcggggctc ttccttctcg tgcttgttgt tcaactgttt gctcagctgt agatcttaac
      781 ccgactaggc ttcatccatg ctcgaagaac tcacagccgg attcccgatt ctgtcctgca
      841 ttctatttct cccggttgtc ggggcggctg tgctctggtt gttcgacgaa gaggatatga
      901 tccggacatc agctctcacc attgcgctgg tggagcttgc gctctctgta tttgtactgc
      961 tgcgtttcgt gccggaatcg gcggccatgc aattcacaga gcgtgtccga tggattccgg
     1021 cattaggtat cagctatcac ctggcggtcg atgggatcag cgtgctgttc gttgggctca
     1081 cggcatttct aaccgtcctg gtcgtggtct actcctggga tacgatccga catcaagtca
     1141 agctgtacat gatgtgcctg ttggcgctcg aaacgacgac catgggcgtc ttcgtatcgt
     1201 tggatttgat cctattcttc gtgttctggg agctgatgct gattcccagc tattttctga
     1261 tcaagttgtg ggggggagga gctgaacgtc attatgccgc gctgaagttc gtcatttata
     1321 cgcttttggg cagcgtgttc atgctggttg gcattgcctt g
//
LOCUS       DASTWE010000289         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_266391, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000289 DASTWE010000000
VERSION     DASTWE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266391"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1265
                     /locus_tag="VFT30_03140"
     CDS             <1..>1265
                     /locus_tag="VFT30_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091649.1"
                     /translation="ISCGEKIDHYETIRRRKDGTLLNMSLTVSPIMDANGRVIGASKI
                     ARDITERKRADEALREAQLRLQRWNIELEQAVNIKTVELLQSQERLRALTTELNLTEQ
                     RERARLATELHDHLQQILVLGKLTIGQGKRAAVGVPACEQIFKKINDIFSEALTYTRT
                     LVSDLSPTVLRDHGLAAALHWLGNYMQKHNLTVTVVGHVNENLKLPEDHVILLFQSVR
                     ELLINSAKHAGTGQATVRMEQHDGHLRIEVRDEGVGFDLAAAGGSETSSEGISSKFGL
                     FSIRERMRALEGSFDLQSALGQGTTATLSLPLATSGKPCEQLIVKSTKTVDAAYTDNP
                     PLTTHHSSLQKNALTRVLLVDDHVMVRQGLRTVLDAYADVELIGEAGTGEEAIRLVDH
                     LRPAVVVMDINMSKMDGIEATRYIKLCYP"
BASE COUNT          306 a          342 c          377 g          240 t
ORIGIN      
        1 atcagctgcg gtgagaagat agaccactac gaaacgattc gtcgccgcaa agatggcacg
       61 ttgctgaaca tgtcactgac cgtttcccct atcatggatg cgaacggacg ggtgatcggc
      121 gcatcgaaaa ttgcgcgaga catcacggag cggaagcggg cggacgaggc gttgcgggaa
      181 gcacaactgc gcttgcaacg gtggaatata gagctggagc aggcggtgaa catcaagacg
      241 gttgaactgc tgcaatcgca ggagcggctt cgtgcgttga ctacagagtt gaacctgacc
      301 gagcagcgcg aacgagcgcg cttggccacg gagctgcacg accatctgca acagatcttg
      361 gtgctgggaa agctcacgat cggacaggga aagcgcgcag ccgtcggcgt gccggcctgt
      421 gaacagattt tcaaaaaaat aaacgatatc ttttccgagg cactcaccta tacccgcacc
      481 cttgtctctg acctcagccc caccgtcctc cgggatcatg gactagcggc ggcactccac
      541 tggctcggca actacatgca aaagcacaat ctgaccgtca cagtggttgg acacgtgaat
      601 gagaacctca agcttcctga agaccacgtc atcttattgt tccaatcggt gcgagagttg
      661 ctgatcaact cggccaaaca tgccggcact ggccaagcca cggtacgaat ggaacagcac
      721 gatggccact tgcggatcga agtccgcgat gaaggtgtgg gtttcgatct tgctgctgcc
      781 ggaggcagcg aaacctccag cgaaggcatt tcgtccaagt tcggactctt cagcatccgt
      841 gaacggatgc gggcgctgga gggttccttc gacctgcaat ccgctctggg acagggcacc
      901 acggcgacgc tcagcttgcc gctggctacc agcggcaagc catgtgaaca gttgatagtg
      961 aagagtacca agacggtgga cgcagcatat acagacaatc cgccactcac cactcatcat
     1021 tcatcactac agaagaacgc cctcactcgc gttcttctgg ttgacgacca cgtcatggtt
     1081 cgccaaggcc tccggacagt gctggatgcg tatgccgatg tggaattgat cggcgaagcc
     1141 gggacggggg aggaggcgat acgactggtg gaccatctgc ggccggcggt ggtggtgatg
     1201 gatattaata tgtcgaagat ggacggcatt gaggcaacga ggtacatcaa gttgtgctat
     1261 ccgga
//
LOCUS       DASTWE010000290         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1526785, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000290 DASTWE010000000
VERSION     DASTWE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526785"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..311
                     /locus_tag="VFT30_03145"
     CDS             156..311
                     /locus_tag="VFT30_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091650.1"
                     /translation="METFRKEITEFISVTERIQSIVARGERLSSDEAGVVRLCATELL
                     EKVPEPR"
     gene            568..>1079
                     /locus_tag="VFT30_03150"
     CDS             568..>1079
                     /locus_tag="VFT30_03150"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEU5091651.1"
                     /translation="MPASGIRGTHDIPLGSHLCLFYRRPAEFLRVTASFLKAGLNENQ
                     LCVWVLPPPLTILLALDELSYHGLNPSVLIATKQLQVVSSTECFFSDGIFEVERALSR
                     VAAFPAMARQLGYSSVRAAGGPGQFVSEACRQAFMCYERHATPIIAELPFIGLCCYAS
                     TECLATDMFD"
BASE COUNT          233 a          274 c          288 g          284 t
ORIGIN      
        1 ggtatgctcg ccagagtaga tcttcctctg cgacaacccg tgatgagtct atgatgataa
       61 agctgtgagt cgatttgaag gtcttgacta ggttgattga gagattatct ccgctgtcca
      121 taggacaact cttcacaacg ctggaggttg gctctatgga aactttcaga aaggagatca
      181 cagagtttat ttcagtgacc gaacggatac aaagtattgt ggcacgaggc gaacgcctct
      241 ccagcgatga agctggagtt gtgcggctct gtgcgacgga gttactcgaa aaggtacctg
      301 aaccgcgcta agccgatttt ctttgttaag aggtagtaca gccgaacggt tccgccgagt
      361 gccatcgcta ctgcggctac ttaggtctct ttgcgaatag agaatatttt gtattttcgt
      421 gtattcatgc ggcattctag ccggtaatcg ggtcaatgtg ggcatggaag gccgggcaca
      481 gtcgactgca ccaggagcca cacgatggcc gaacgatgct gcctgattga gttctatcac
      541 ctcctggctt aaggcgaggg ggtatccatg ccagccagtg gtattcgtgg cacgcatgac
      601 attcctttgg gatcgcatct ctgtctattt taccggcgac ccgcagaatt tctacgcgtc
      661 accgcatctt ttctgaaagc agggctaaac gaaaatcaat tatgtgtctg ggttcttccc
      721 ccaccgttga ccatcctctt agccctcgac gaactgtcgt atcacggcct gaatccatcg
      781 gtcctgatag ccacgaaaca gcttcaagtc gtgtcctcca cagagtgttt cttttccgat
      841 ggcatcttcg aagtggagag ggccttgagc cgggtggccg cgtttcctgc catggctcgc
      901 caactcggat attcgagtgt tcgtgcggcg ggtgggccag gccaatttgt gtctgaagcc
      961 tgtcgccagg cttttatgtg ctatgagcgc cacgcgacgc caatcatcgc cgaacttccc
     1021 ttcatcgggt tatgctgcta tgcctccaca gaatgtttag cgaccgacat gtttgacat
//
LOCUS       DASTWE010000291         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_492767, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000291 DASTWE010000000
VERSION     DASTWE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492767"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="VFT30_03155"
     CDS             <1..1897
                     /locus_tag="VFT30_03155"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HEU5091652.1"
                     /translation="VSLGGLEALLKAVPASAQETVEGADDEKEKVEPGGRRRPVLGFE
                     SVPVSSTDEVVVPKGYTAEVLIAWGDPVSSGPAFKPDASNTADEQARQWGMHNDGVVY
                     FPIVGSQRGLIVQNNEYTDDGLLFPDGVNNWIAEKTRKSLNAHGVSIIEVTKQSGFPW
                     DRRHRREKWDVVRPSPFARRITGATPIEIGGPAAGDPRLVTSEDPTGKRVLGTLNNCA
                     MGYTPWGTYLACEENFNGYFRKNGTQTDLEKRYGIMASGSGYLWHTTDKRFRVDVEPN
                     EPNRFGWVVEIDPFRPRSMPVKRTALGRLKHEGAWVQEARNGRIVVYMGDDERNEYIY
                     RYVSNLPWRQAQHQGISPLDDGILYAAKFHTDGAGEWLPLTPDNPRLAGWSLNDILIN
                     TRGAADAAGATMMDRPEWIDTFPRSLTAIATLTNNNRRGTTPPSVNNPNGTTSAGSSR
                     PPVDAINPRTVNNYGHIIRWHYSQDWTEPTFGWDIFALCGDPANLTHGSTIVGDKYGS
                     PDGIYVDPSGLLWIQTDVSTTTINGGAYAGFGNNMMVAADPETGETRRFLVGPNQCEV
                     TGVMMTPDRRSMFVGIQHPGERPDDQPADPNNPKQFSSWPDGPNGGRPRSALIVITKD
                     DGGRIGS"
BASE COUNT          469 a          543 c          614 g          400 t
ORIGIN      
        1 ggtgtcgctc ggtggtctcg aagcactatt gaaggctgtg cccgcttcag cccaagaaac
       61 ggtagaaggc gccgacgatg aaaaggaaaa ggttgagccg ggaggacgtc gcaggccggt
      121 gcttgggttt gaaagtgtgc cggtctcgtc caccgatgaa gtcgtggtgc cgaaaggcta
      181 tacagccgag gtcctcattg cgtggggcga tccggtttcc agcggcccgg cctttaaacc
      241 ggatgcgagt aatacggccg atgagcaagc tcgtcagtgg ggtatgcata acgacggagt
      301 cgtctacttt ccgatcgtcg ggtcccagcg tgggttgatc gtgcagaaca acgaatatac
      361 ggacgacgga cttctgtttc cggacggcgt caacaactgg attgctgaga agacgagaaa
      421 atcgctcaat gcccacgggg tctctatcat cgaagttacc aagcaatcag ggtttccctg
      481 ggatcggcgg catcgacgcg agaaatggga tgtcgtgcga ccgtctcctt tcgcccgtcg
      541 cataactggc gcgactccga tcgaaatcgg tggaccagcg gccggcgatc ctcggttggt
      601 cactagtgaa gatccgaccg gcaagcgcgt gttggggacc cttaataatt gcgcgatggg
      661 gtataccccc tggggaacgt atttggcctg tgaagaaaat tttaacggct acttcagaaa
      721 aaacggcacg cagacggatc tcgagaaacg ttatggcatc atggcgtcgg gatctggata
      781 cttatggcac accacggaca agcggttccg ggtggatgta gaaccgaatg agccgaatcg
      841 cttcggctgg gtggtcgaga tcgatccgtt tcggccccgt tcgatgccgg tcaagcgcac
      901 ggcgttgggg cgtctcaagc acgaaggagc ctgggttcag gaggccagga acggtcgaat
      961 agttgtgtac atgggagatg atgaacggaa tgagtacatc tatcgttatg tgtccaactt
     1021 gccgtggcgg caagcgcagc accaaggaat cagtccgctc gacgacggga ttctctacgc
     1081 cgcgaagttc catacggacg gtgcgggcga atggctccca ctcactccag ataacccgcg
     1141 tctggccggg tggtcgctca acgacattct catcaatacg cgcggcgccg ccgatgcagc
     1201 cggtgcgacc atgatggatc gccctgaatg gatcgacact tttcctcggt cccttacggc
     1261 gatcgcgacg ctcaccaata acaaccggcg cggcacgacc ccaccgtcgg tgaacaatcc
     1321 gaatggcacg accagcgccg gatcctcccg gccaccagtg gatgcgatca atcccagaac
     1381 agtcaacaac tatggccaca tcatccgttg gcactatagt caggattgga cggagccaac
     1441 gtttgggtgg gatatctttg cattgtgcgg cgatcccgca aatctaactc atgggtccac
     1501 gattgtgggt gacaagtacg gctcgccaga cggcatctac gtcgacccga gtgggttgct
     1561 ctggattcag acggatgtgt cgacaacaac aatcaatggc ggtgcctacg ccggtttcgg
     1621 caacaacatg atggttgcag ccgatccaga aacaggggag acccgccgct ttctagtggg
     1681 gccgaatcaa tgtgaggtca caggagtcat gatgacaccg gaccggcgca gtatgttcgt
     1741 cgggattcaa catccaggag agcggccgga cgatcagcca gctgatccaa ataacccgaa
     1801 acaattcagc tcttggccgg atggtccaaa cggaggccga cctcggtcgg cgctgatcgt
     1861 cattactaaa gacgatggcg gcaggatcgg cagctgagac taccacagtg caaaggaagc
     1921 cgagatgcag cctcgaccat gtatatcaac atctcggggc aggagttcgg acatcaccgc
     1981 atttgtaatc gttatggaac ctcgcacaaa gcttttgggc tgcagt
//
LOCUS       DASTWE010000292         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1365232, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000292 DASTWE010000000
VERSION     DASTWE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365232"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFT30_03160"
     CDS             complement(<1..650)
                     /locus_tag="VFT30_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5091653.1"
                     /translation="MSSRFFIERPIFASVLSIVIVVIGLVALIKLPIAQFPNISPPVV
                     QIEADYPGASAEVAADSVARTIEVQLPGIDNLLYYDSTSTNDGHVSIRLTFEIGTNVD
                     IAQVQTQNRVKLAEPQIPPEVVRQGISILKFSPDLLAVVALSSSDPTHDTVFISNYAL
                     LRVIDNLKRIPGVGQALVFGQQNYSMRLILNPVRMAQLGLTPSDIANVVREQNRDFP"
     gene            complement(647..1903)
                     /locus_tag="VFT30_03165"
     CDS             complement(647..1903)
                     /locus_tag="VFT30_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5091654.1"
                     /translation="MSIWDHYLFRYVAVVIVSAAIAGVLGCKDDAGSTPSPPSPIPQV
                     EVVSVMARTVPDEPEFIGQAEASRPVEIRSQVTGILKAVLFIEGREIKKGEKLYQIDP
                     VPFEGVYQSAKARIAEAEARLVQARQDLARVKPLLEAQAVSQKDVDDAIAEDLAAKAA
                     LQRAKADLIKAKFDFDNTVITAPIKGLIERSRYYEGRLVSAQTDLLTVINRIDPMYVM
                     VNVPESFLLKRRQDAIAVSLQHPGLAQLQGTIIFADGSTYNHEGFLDFTDVGLRTETG
                     TRRARFVVANPDGVLLPGQFVRVRFKGTMKDHALLVPQRAVHQGPQGQIVFVVGEEDK
                     VEIRPIKASRWQDKEWIIESGVRSGERVIVSGFHMVKPGAPVKPVPAAEPAAESTANG
                     KTGNKMSNGGADHEAEAKPEATKDPQ"
BASE COUNT          424 a          598 c          554 g          450 t
ORIGIN      
        1 ggaaaatcgc ggttctgttc gcgaaccaca ttggcgatgt cgctcggtgt cagccccaat
       61 tgcgccatgc gcaccgggtt caaaatgagg cgcatcgagt aattttgctg tccgaagacc
      121 aaggcctgtc cgacacccgg gatacgcttg aggttgtcga tgacacgcaa caacgcataa
      181 ttcgagataa ataccgtgtc gtgagtgggg tcgctggaac tcagtgcgac gaccgccaag
      241 aggtcgggag aaaatttcaa gatgctgatt ccctgccgaa cgacttcagg cggaatctgc
      301 ggctcggcca gcttcactcg gttttgcgtc tgcacctggg cgatatccac gttcgtgccg
      361 atttcgaacg tgagtctgat actgacatga ccgtcgttcg tgctggtgga atcgtagtac
      421 aggagattgt cgatgcccgg caactgcact tcgatcgtcc gcgccaccga atccgccgcg
      481 acttccgcgc tggctcccgg atagtcggcc tcgatctgaa cgacgggcgg gctgatatta
      541 gggaattgag caatggggag cttgatgagc gcaacgagcc cgatgacgac gatgacaatg
      601 gagaggaccg acgcaaagat cggccgttcg atgaagaagc gtgaactcac tgtggatcct
      661 tcgtggcttc tggcttggct tccgcctcat ggtcagcccc gccgttggac atcttgttac
      721 ccgtttttcc gttagccgtg gactcggcag cgggctccgc agccggaacc ggtttgacgg
      781 gcgctccggg cttcaccatg tggaagccgc tcacgatcac gcgctcgccg gaccgcacac
      841 ccgactcgat aatccattcc ttgtcttgcc agcggctcgc cttgataggg cgaatctcca
      901 ccttatcttc ttcacccacg acgaatacga tctggccctg gggcccttga tggaccgctc
      961 gctgcggcac caacagggca tggtctttca tcgttccttt aaagcgcacc ctgacaaatt
     1021 gcccgggcag caacactcca tcgggattgg cgacgacgaa tcgggctcgc ctagttcctg
     1081 tttcggtccg caggccgacg tcggtgaagt cgaggaaacc ttcgtgattg tacgtgctgc
     1141 cgtccgcaaa gatgatggtg ccttgcagct gcgcgaggcc tggatgttga agactcaccg
     1201 cgatggcatc ctgccgccgc ttaagcagaa aactttccgg cacgttgacc atcacgtaca
     1261 tcgggtcgat acgattaatg acggtcagca aatcggtttg ggcagagacc agtcggccct
     1321 cgtagtaccg actccgctcg atgagcccct tgatcggagc tgtgatgacc gtattgtcga
     1381 agtcgaactt ggctttgatc aagtcggcct tagctctttg cagagcagcc ttcgccgcca
     1441 agtcctcggc gatcgcatca tccacatcct tttggctgac ggcctgcgct tcgagcagcg
     1501 gcttgacacg ggcgagatct tgcctagctt ggactaagcg cgcttccgcc tccgcgatcc
     1561 tggccttcgc gctctggtac accccttcaa acggcacagg atctatttgg taaagcttct
     1621 cgcccttttt gatctctcgt ccttctataa acagcacggc tttcagaatt cccgtgactt
     1681 gtgagcgaat ttcaaccggg cgagacgctt ccgcctgccc tatgaattcc ggttcatccg
     1741 gcacggtccg cgccatcacc gacacaactt ccacttgcgg aatcggagaa ggaggtgaag
     1801 gagtcgagcc cgcatcatcc ttgcaaccga gtaccccagc tatcgcggct gatacaatca
     1861 ccacggcaac atatctgaac aggtaatgat cccaaatact catgtacgtt gcaccttgat
     1921 tggattttcc cctcaaagac aaacagttca ttgtaccgat gcaagtgaga cggcggcaag
     1981 cccaagacag acaagacttc tcagcatcat ggtgacgagt gaaggc
//
LOCUS       DASTWE010000293         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_718927, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000293 DASTWE010000000
VERSION     DASTWE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718927"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /gene="thrS"
                     /locus_tag="VFT30_03170"
     CDS             <1..1895
                     /gene="thrS"
                     /locus_tag="VFT30_03170"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5091655.1"
                     /translation="ILTGKTVGDALSELGVGDRDILAAKVDGAVVDLSRPLSRSSVVE
                     PLRFDSAEGREVYRHSSTHIMAQAVKELFPSAQLTIGPALEDSFYYDFAFERPFTPED
                     LERIEERAAEIIRRNLTITRREFSKQEAIDFFRARGEHYKVELIQGFPDGEPITAYTQ
                     GDFVDLCRGPHLPTTGSIGAIKLLTTAGAYWRGDERNPMLQRIYGTSFPTKGDVDAYL
                     ARLEEIKRRDHRKVGKELDLISIQDEIGPGLVLWHPKGAAVRLLIENFWREQHIRDGY
                     QLVYSPHTARLDLWKTSGHLDYYRENMFPSMKLEGSEYQLKPMNCPYHIMIYQSHLRS
                     YRDLPIRYGELGTVYRYERTGVLHGLMRVRGFTQDDAHLFCRPDQMEHEVSRVLDFTF
                     FVLQTFGFHQFEVFLSTRPKESVGGDEHWTLATSALEAALKSRNISFHLDPGGGAFYG
                     PKIDIKIKDALGRSWQCSTVQVDFNNPERFELSYIGEDGKAHRPIMIHRALMGSIERF
                     FGILIEHYGGAFPTWLAPVQAVVMNITDHQQDYAASVVAQLRTAGFRAEADLRNEKIG
                     FKIREAEKAKIPFMLVAGNREMQDGTLSVRGRSGSNLGNKTVAEVLDLLQAEVKHTQR
                     ELQHIH"
BASE COUNT          479 a          481 c          520 g          430 t
ORIGIN      
        1 acattctgac tgggaagacg gtaggtgacg ctctatctga gttaggagta ggagaccggg
       61 atatcctggc agccaaagta gacggagcgg ttgttgatct gtcgcgtcct ttgtctagga
      121 gttcggtagt cgaaccgctg aggttcgaca gcgcagaggg tcgtgaggtg taccgtcaca
      181 gcagtaccca catcatggct caggcagtaa aagaactttt cccgtccgca caactgacca
      241 tcggcccagc cctggaagat agtttttatt atgactttgc cttcgaacgt ccatttaccc
      301 ccgaagactt ggagagaatc gaagaacgcg ccgcagaaat catcaggcgt aatctcacta
      361 tcacaaggcg ggagttttcg aagcaggagg ccatagattt tttcagggcc cgtggagaac
      421 attacaaggt cgagctgatt caaggttttc ccgacggaga accgatcacc gcctacacac
      481 aaggcgactt tgtcgatctc tgtcgcggac ctcatctccc taccaccggc tccattgggg
      541 ctataaaatt actcaccacg gccggagcgt attggcgtgg cgatgaacgt aatccaatgt
      601 tacagcggat ctacgggaca tcctttccga caaaaggaga cgtagatgcc tacttggccc
      661 ggctagagga aattaagcgg cgcgaccaca gaaaggtcgg aaaagaactg gatttgatca
      721 gcatccagga tgagatcgga cccggccttg tactttggca tccgaaaggc gcggcagtcc
      781 gcctgttgat tgaaaatttc tggcgagaac agcatattcg ggatgggtac cagctggtct
      841 attcgcctca taccgcacgc cttgacctct ggaaaaccag tggtcatctc gactactacc
      901 gcgaaaacat gttcccatca atgaagctgg aaggcagtga gtaccaactg aaaccgatga
      961 attgtccgta ccatatcatg atctatcaga gtcatcttcg cagttatcga gacctcccca
     1021 ttcgctacgg ggaactgggg acggtctacc gctatgaacg gaccggcgtt ctgcacgggc
     1081 ttatgcgggt ccgcggattc acgcaggatg atgcccatct cttctgccgt ccggatcaga
     1141 tggaacacga agtcagccgc gtactggact ttacattttt cgtgttacag acttttggat
     1201 tccatcaatt tgaagtgttt ttgtccaccc ggcccaaaga atccgtaggc ggtgatgagc
     1261 attggacctt ggccaccagt gcattagaag cagcactgaa aagccgtaat atctcctttc
     1321 atcttgatcc gggagggggg gcattttacg gcccgaagat cgacatcaag atcaaagacg
     1381 cgctgggccg ctcatggcaa tgttccacgg tccaggtcga tttcaacaat ccggagcggt
     1441 ttgaattaag ttacatcgga gaggacggca aagctcatcg ccccatcatg atccatcgcg
     1501 ccttgatggg atccatcgag cgtttttttg gcattctgat cgagcactac ggaggtgcgt
     1561 tcccgacctg gctggctccg gtgcaagcgg tggtcatgaa catcaccgat catcagcaag
     1621 actacgccgc gtccgtcgtc gcgcagttga ggacggccgg ctttcgtgcc gaagcggatc
     1681 ttcggaacga aaaaattggg ttcaagattc gtgaggccga aaaagcaaaa attccgttca
     1741 tgttggtagc agggaatcgc gaaatgcaag atgggacgct ctcggtacga ggccgaagcg
     1801 ggtcaaattt agggaataag acagtggctg aggtgttgga tcttttgcaa gctgaggtca
     1861 aacacacaca gcgagagctt caacacattc attaaaaaag aggtggccta
//
LOCUS       DASTWE010000294         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1235989, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000294 DASTWE010000000
VERSION     DASTWE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235989"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..639
                     /locus_tag="VFT30_03175"
     CDS             241..639
                     /locus_tag="VFT30_03175"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091656.1"
                     /translation="MKLQDKNDHPHGLPDKHFDTFFTTDKPIIFAYHGYPWLIHRLTY
                     RRTNHKNLHVRGYKEEGTTTTPFDMVFLNDMDRYHLAADVIERVGLGSAAAYAKQAIR
                     DKRIEHKAYIRKHGEDMPEIRQWKWDARKA"
     gene            857..1828
                     /locus_tag="VFT30_03180"
     CDS             857..1828
                     /locus_tag="VFT30_03180"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEU5091657.1"
                     /translation="MGRRVTYDRCVVGIIAIVCLLGGKAYAGDDKRSNDATPKEVLDC
                     RYQQVSALSEEGAIGETFPSDDLFRPLLADPKQPQFFALWQSTQSRLERTNANIGSIG
                     IGENFGFYTRRKGCNGWQISLLTGIFAQFDLDSSNAALINVDFNVGIPLTWRHGNWSA
                     RLRYYHQSSHIGDEFLAANPGFQSIGLQFEEVDMIVSYDFWKWLRLYGGGAVMVNRQP
                     SSIHRNTAQWGFEARTPTPLGQSYMLGLLSNPILFTPLLTADFKSVEEQDWYINTNLL
                     MGMDMSRAGYFKRLRILFNYYHGYNPYGQFFYSQKTESFGAGAYFMF"
     gene            1914..>2789
                     /locus_tag="VFT30_03185"
     CDS             1914..>2789
                     /locus_tag="VFT30_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEU5091658.1"
                     /translation="MNNKVSFSIWYILLAIMAVVFVHDFILALNKVEELPYSEFKKLV
                     VGGKVAEVSVTNQVLTGKLKPEGESKEQKAFATVRVEDPDLVRELNQHGVTFTGVIES
                     TFWRDLLSWVVPVALFVGIWFFIFRRLGQAQGGFMQVGQSKAKIYVEKDIKVTFADVA
                     GVDEAKEELREVIEFLKTPEKFTKLGGKIPKGILLVGPPGTGKTLLARAVAGEAGVTF
                     FSISGSEFVEMFVGVGAARVRDLFEQAKGKAPCIIFIDELDALGKARGVGPMAHEERE
                     QTLNQLLVEMDGFDPR"
BASE COUNT          651 a          722 c          781 g          635 t
ORIGIN      
        1 caacgcggcc ctccgtgcca attcttccgc catagacatt gaatcgctgc accgcgacgg
       61 gcgagtagaa accgtacgtc gtattcatgc ggtacctgaa cacatgatcc ccacccagcc
      121 atatcgcttt ctgcactccg atgcctagcg ttgtcgtaaa agagccaccc gcggccgtgc
      181 agctcttgcg cggatacgtg ccgtcgctca agagccgggg tcgtcaatat agtcgatctg
      241 atgaagttgc aggacaaaaa cgatcatccg cacgggttgc cggacaagca tttcgatacg
      301 tttttcacca ccgataagcc gatcatcttc gcatatcacg gctatccctg gttgatccac
      361 cggttgacgt accggaggac caaccataag aatctgcacg tgcgtggcta caaggaagag
      421 ggcacgacca ccacaccgtt cgacatggta ttcctgaacg atatggaccg gtatcacctg
      481 gccgccgatg tgatcgaacg cgtcggcttg ggatcggcgg cggcctatgc caagcaggcc
      541 attcgcgaca agcggatcga gcacaaggca tacatcagga agcacggaga agacatgcct
      601 gagatccgtc aatggaaatg ggatgcaaga aaagcttgat gggaaggaca cggtcatcga
      661 cgaatgcgac cggctgacca tccgttcttc gcatcccatc acgtctttca ttattccgac
      721 atgatttgaa aagctccacc aatcggcgcg atgcgcgaca aaccatgtga atttcccatc
      781 agaagtatgc tatttgaacg cgtttgggtt gcgtcacaca agaacgtcgg caagaacgtc
      841 ggatgggagg agcggcatgg gtcggagagt tacgtatgat cgatgtgtcg tcgggataat
      901 agcaatcgtt tgtctgctgg gaggaaaagc ctatgcaggt gatgataaaa ggtctaatga
      961 tgcgacgcca aaagaggtgc tggattgtcg gtaccagcaa gtttctgcgc tttcggaaga
     1021 gggagcgatt ggtgaaacat ttccgtcgga cgatctcttt cgcccacttt tggccgaccc
     1081 caaacagccg caattctttg ccctctggca atccacgcaa tcacgccttg aacggaccaa
     1141 tgcgaacatc gggtcgattg gtattggcga gaattttggg ttttatacca gacgcaaggg
     1201 atgtaatgga tggcagatca gcctgctgac cgggattttc gcgcaattcg atttggattc
     1261 gtcgaacgcc gcactcatca atgtcgactt caacgtaggc ataccgctga cctggcgtca
     1321 tggcaactgg tccgcacgct tacgctatta tcaccagagc agtcacattg gagatgagtt
     1381 cctggcggcc aatcctggct ttcagtcgat cggtctccag tttgaagagg tcgacatgat
     1441 tgtgtcttat gacttctgga aatggttacg tctctatggg ggaggagcgg tcatggtcaa
     1501 tcggcagcct tccagtatcc accgcaatac cgcacaatgg gggttcgaag cgaggacacc
     1561 gacgccattg ggacaatctt atatgttagg tctgctctcg aatccgatcc tcttcacgcc
     1621 cctcctgacg gccgacttta aatctgtcga ggagcaagac tggtacatca acactaactt
     1681 gctcatgggc atggatatgt cccgggctgg atattttaag cgcctgcgga ttcttttcaa
     1741 ctactatcac ggatataatc cctatggaca attcttctac tcgcagaaga ctgaatcatt
     1801 cggagccggc gcctatttca tgttttagag ttgtgcgagg aggcgggcct cgatcatgtt
     1861 caacctaccg gcgccgagac gaattctctt caacgccgtg agggacaacg ccgatgaata
     1921 acaaggtctc gttctccatc tggtacatcc tcctcgccat catggcggtg gtgttcgtgc
     1981 atgacttcat acttgctctc aacaaagtcg aggaactccc ttacagtgaa ttcaaaaagc
     2041 tggtggtagg cggcaaggtg gctgaagtct cggtaaccaa tcaagtactg accggaaagt
     2101 tgaagcctga gggagaatcc aaagaacaaa aagcctttgc gaccgtacgg gtcgaggacc
     2161 cggatctcgt tcgtgagctc aaccaacatg gagtgacttt taccggcgtc atcgagtcca
     2221 cattttggcg ggatctcctg tcgtgggtcg tcccggtcgc cctctttgtc ggcatctggt
     2281 ttttcatctt tcgccggtta gggcaggctc agggcggttt catgcaagtt ggccaatcga
     2341 aggccaagat ctatgtggag aaggatatca aggtgacgtt cgcggatgtg gcgggcgtgg
     2401 acgaggccaa agaagaactg cgtgaggtga tcgagttcct caagacgccg gagaaattca
     2461 ccaagcttgg cggcaagatt cccaagggta ttctgcttgt cggaccgccg ggaaccggca
     2521 aaacgctgct ggcccgcgcc gtggccggcg aagccggcgt gacgttcttc agcatcagcg
     2581 gctcggagtt cgtcgagatg ttcgttggtg tcggcgcggc gcgtgttcgc gatttgttcg
     2641 aacaggcgaa gggcaaagct ccttgcatca ttttcatcga tgaattggat gcgctgggaa
     2701 aagcacgcgg cgtggggccc atggctcacg aggaacgcga gcaaactctc aaccagctgt
     2761 tggtcgagat ggatgggttc gatccacgg
//
LOCUS       DASTWE010000295         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1042176, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000295 DASTWE010000000
VERSION     DASTWE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042176"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..437)
                     /locus_tag="VFT30_03190"
     CDS             complement(99..437)
                     /locus_tag="VFT30_03190"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEU5091659.1"
                     /translation="MSISSTDHNPALDGSDCRKVMVDQSFFIHLWEDRTRGEQWVPSY
                     DTKGLALLSDEFLRIASNNAVENGQRIFEFKAVRPGVYQLIFEKRMGWKFTAEDRRLF
                     IIEAGRSSEN"
     gene            complement(492..809)
                     /locus_tag="VFT30_03195"
     CDS             complement(492..809)
                     /locus_tag="VFT30_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5091660.1"
                     /translation="MDGFQKVAQIDDLPPGKSKIVKINDRPIALFNIEGKLYAIHNSC
                     PHEGGPLIEGRLKGYVIACPWHDLAFDIRNGQGIDGGGYCVGSYEVRVDGNDILIGPR
                     RKI"
     gene            complement(1101..1601)
                     /locus_tag="VFT30_03200"
     CDS             complement(1101..1601)
                     /locus_tag="VFT30_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU5091661.1"
                     /translation="MASDQVQTSNREQRKKRIIMMILLALFLMSASVFLVERKESSII
                     VVTFPSGVELEAEVADTPEKLLFGLAFRDALPPNAGMLYIFEQNGMHRVTTKEYRFPI
                     DILWVDESHHVVHMLEHAEPCVQDPCPFFGPPPEAVRYVIQAESGFIKKAGVAKSDEL
                     KYALRM"
     gene            1828..2175
                     /locus_tag="VFT30_03205"
     CDS             1828..2175
                     /locus_tag="VFT30_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5091662.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAEAKGNHVGDGQGV
                     GDNIYVIAHDRCTECVGHFDEPQCAAVCPVDNCCISDPLYPETTDVLLERAKTLNPDK
                     AIDPAKVWSGVRN"
     gene            complement(2275..>2663)
                     /locus_tag="VFT30_03210"
     CDS             complement(2275..>2663)
                     /locus_tag="VFT30_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091663.1"
                     /translation="ETGDLETGSVVGQPFKELTVDASFFSIEIGNMKTWYPPTTVIDF
                     KSRPGAPVLLKVTNNSAAEHGFHLSSAVNQSGPFSLEAKLVLKPGETRYIGVPTSDLF
                     YASGSVLEYRCHLHAAHVGGKLVMLK"
BASE COUNT          629 a          703 c          663 g          668 t
ORIGIN      
        1 cggcatctcc gaattggaac ccccggtcat cgattgaaat ggtcgcctcc tcccagggaa
       61 gaaagcggcc attgatgaac gcgatgtcgg gcattggctc aattctcgga tgatcgccct
      121 gcttctatga tgaacaagcg ccgatcttcc gcagtaaatt tccatcccat acgcttttcg
      181 aaaatcagct gataaacacc tggccgcacg gctttaaact cgaagatgcg ctgcccgttt
      241 tctacggcgt tattgctcgc gatacggagg aattcatcgc tgagaagcgc aagtcctttg
      301 gtgtcgtatg acggcaccca ctgttcacct ctggtgcgat cctcccatag atggatgaag
      361 aatgactggt cgaccatgac cttgcgacag tcgctgccgt ctaaagcagg attgtgatcg
      421 gtactggaaa tactcacatc gataccctga tccgtgtcgt gaagaacaac cgttcggccg
      481 gatttccctt tttatatttt gcgcctaggg ccgatcagaa tatcgttccc gtccacgcgg
      541 acttcgtagc ttcctacaca gtacccgccg ccgtcgatgc cttgcccatt tctgatatcg
      601 aacgccagat catgccaggg gcaggcaatg acgtaccctt tgagtctccc ctcaatgagc
      661 ggcccgccct cgtggggaca gctattatgg atcgcgtaca atttcccctc aatgttgaac
      721 aacgcgatcg ggcggtcatt gatcttgaca atctttgact ttcccggagg taagtcatcg
      781 atctgtgcaa ccttttgaaa cccatccatt gtgcgtattt cctcttgctg tctcttagcc
      841 tgcattattt atgaacgctt ctgctgcaat cgcgaattcg ctgctgcgac gagccttcgg
      901 cgggcaggct cgcccctcgc aacctcgata tactgtttcg agtatgcctc ggttcttcgg
      961 ggttccacgt gtcaagacac tgcccgttca ccactcgcgg gcgtccacaa acgtggcgct
     1021 cattatcctt cgcgccgtgg acctcgccac gcgactgcgc aatttcgcca cgaaccgtca
     1081 tgaataatgc gggccagtcc ctacatacga agggcatact tgagctcgtc gctttttgca
     1141 actccggcct tcttaatgaa ccccgattct gcctgtatca cataccgaac ggcttcgggt
     1201 ggtggcccaa aaaatgggca aggatcttga acacatggct ctgcatgctc cagcatatgc
     1261 acgacgtgat gactctcatc cacccacaga atgtcgatgg ggaatcgata ttccttggtt
     1321 gtcacgcgat gcatcccgtt ttgctcaaag atataaagca tgccggcgtt tggaggcaat
     1381 gcatcgcgaa aggcaagccc aaagagcaac ttctccggcg tgtcggccac ttcggcttcc
     1441 aactcaacac cgctcgggaa tgtgacgact atgatgctcg attccttgcg ttcgaccagg
     1501 aaaaccgaag cgctcatcag gaaaagggca agcagaatca tcatgatgat tcgcttcttc
     1561 cgctgttctc gattgctcgt ttggacttga tcagatgcca ttgatggtga ccgaagcttt
     1621 ctttctttgc tgggcccaat ggctcatgcc gaataatgac ctaggtcttc tgacctgatt
     1681 ccgttcagtt gacttagaaa agaatcgcgg aataaaatgt ttttctgcgt ggctattcgc
     1741 cataagtcga caggaagcac tatggtgtga gccgcgcgaa cttgtcaatg ttctatagtc
     1801 ttttggagtg agaaggaggc ttgggtcatg gcattattga ttaccgacga atgcatctct
     1861 tgtggagcat gtctgccgga atgtccaaat gaagcgatct tcgaaaccag gagcgacgcg
     1921 gaagccaaag gcaatcacgt tggcgatggg caaggcgtcg gcgataatat ttatgtgatc
     1981 gcacatgacc gctgcactga gtgtgtcggg cactttgatg aacctcagtg tgctgcggtt
     2041 tgccccgtcg ataattgttg tatttcagac cctctctatc ctgaaacgac ggacgtactt
     2101 ctggaaaggg cgaagacttt gaatcccgat aaagcgatag atcctgccaa ggtatggagt
     2161 ggggtaagaa actgactctc tcaagtgaat ttgtttaagg gcggttgtgg gagaaaataa
     2221 aaaggcctcg gtgaaactca ccgaggcctt tttcatcgtc cgaattgaaa taagttactt
     2281 cagcatcacc aacttgccgc cgacgtgggc cgcatgcaaa tggcatcgat attcgaggac
     2341 actccccgat gcataaaaca aatcgctggt tggaacaccg atgtaacgcg tttccccggg
     2401 cttgagcact aacttagctt ccaaggaaaa tggtcccgat tgattgactg cagaactcag
     2461 atgaaagcca tgttcggcag cggaattatt tgtcactttt aacagaactg gagcaccagg
     2521 acgcgactta aaatcgatga ctgtcgtggg agggtaccaa gtcttcatgt tgccaatttc
     2581 gatagagaag aacgaagcat ctacggtcag ctccttaaac ggctgtccga cgacagaacc
     2641 ggtttccaga tcaccggtct caa
//
LOCUS       DASTWE010000296         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_234630, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000296 DASTWE010000000
VERSION     DASTWE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234630"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..>1110)
                     /locus_tag="VFT30_03215"
     CDS             complement(97..>1110)
                     /locus_tag="VFT30_03215"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEU5091664.1"
                     /translation="EAGKMTLREQVKAELHQSIVEVGTTVCRTAAEARAELVRLRQGV
                     MELAGRNGLKIASAGTHPISSWVDQEITPLERYAGVKQDMQILAQQLLIFGTHIHIGI
                     EDQDFLIDAMNVVRYFMPHVLCLSTSSPFWIGQDTGLKSYRSIVFRNFPRTGIPPFFF
                     SYSDFARYRDTLVQTRSIPDGTKIWFDARPNHSYPTLEFRICDVCTRVDEAVCIAAIF
                     QALIFKLWKLRRDNMTFRVYSHSLIEENKWRAVRYGLDGKLIDFGKQVELPARELIRE
                     MIEWFIGDVADELGSRKEIEYAYHIMDEGTSADRQLATFRRTGSLQAVVDQLIEETQE
                     GVL"
BASE COUNT          263 a          300 c          285 g          262 t
ORIGIN      
        1 acatgcagac ctgcttcttt gcatgcctgt agaaacgccg ctcccttgaa gtagctggag
       61 agacaaagaa tggtttgagt catgagcttc tcctattcac agcacgcctt cttgtgtctc
      121 ctcgatcagc tgatccacca ctgcctgcaa actgccggtg cgcctgaaag tcgccagttg
      181 gcggtcggca ctggtaccct catccataat gtgataagcg tattcgatct ctttgcggct
      241 tccaagctca tccgccacat cgccgataaa ccattcgatc atttcgcgga tcaactcgcg
      301 ggcggggagt tccacctgct tgccgaagtc aatgagcttg ccatccagcc catagcggac
      361 ggcgcgccac ttattctctt cgatcaaaga atgtgagtac acacgaaatg tcatattatc
      421 gcggcgaagt ttccagagct tgaagatcag agcctggaag attgcagcta tacagacggc
      481 ttcatccacc cgtgtacaca catcacagat gcgaaattcc agtgttgggt aactgtggtt
      541 gggacgcgca tcgaaccaga ttttggttcc gtcggggatg ctcctggttt gaacgagcgt
      601 atcacggtat ctggcaaaat cggaataaga aaagaagaag ggcggaattc cggtgcgggg
      661 aaaattgcga aagacgatgc tccggtacga cttgagtcct gtatcctggc cgatccagaa
      721 cggcgaactg gtggataagc acagcacatg tggcatgaag tagcgaacca cgttcatcgc
      781 atcgatcaga aaatcctggt cttcaatacc aatatgaata tgagttccga aaataagcaa
      841 ctgctgtgcc aggatttgca tgtcctgctt caccccagca tagcgctcga gaggtgtgat
      901 ttcctggtca acccatgatg agattggatg tgttccggcc gaggcaattt tcaacccgtt
      961 gcgaccggca agctccatca cgccctggcg caggcgcacc aactcggcgc gcgcttcggc
     1021 ggccgtgcga catactgtcg ttccaacttc gacaatcgat tggtgcagtt cggctttgac
     1081 ctgctcacga agtgtcatct tgcctgcttc
//
LOCUS       DASTWE010000297         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1010222, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000297 DASTWE010000000
VERSION     DASTWE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010222"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..>2276
                     /locus_tag="VFT30_03220"
     CDS             216..>2276
                     /locus_tag="VFT30_03220"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEU5091665.1"
                     /translation="MRIDRRFTRRGQNPYEGITFVKRLSEIRNPDGSTVFKLENIDVP
                     EAWSQLAVDILAQKYFRKAGVPQRDQNGQPVIGPDGKPVLGGERDARQVFERMAGCWT
                     HWGKSYGYFKTPEDAESFYDEMCYMLAHQMAAPNSPQWFNTGLHYAYGLSGPAQGHYY
                     VDPQTCEVVKATNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTN
                     FSKLRGDGEGLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPDIEE
                     FIDWKVIEEQKVAAMVTGSKICAQRLNAVLKACHMVDGQGSLRLDLDPKTNPVLREAL
                     TLARRDMVPEAYIQRMFSYAQQGFTHFVFHEYDTNWDGKAYQTVSGQNSNNSVRIPNE
                     FFAALETDGDWHLKRRTNGKVCKTVKARELWDRIAWAAWICADPGTQYDTIINEWHTC
                     PEDGRINASNPCSEYMFLDDTACNLASLNLTKFYTADGQFELENFRHAVRLWTIALEI
                     SVLMASFPSRSIAEKSYQFRTLGLGYANLGTVLMRQGIPYDSPKALAICGAITAIMTG
                     EAYGTSAEMAAELSPFPGYAKNREHMLRVIRNHRRAAYQASYVEYEGLTIAPAGIRPE
                     HCPPDMLLAARRAWDHALELGTAYGYRNAQVTVIAPTGTIGLVMDCDTTGIEPDFALV
                     KFKKL"
BASE COUNT          526 a          569 c          644 g          537 t
ORIGIN      
        1 ttggaggggt ccaatttcac tggttatttc tgtggataac ttcgttggta agtgactaaa
       61 ataggctacg agattcgcat tgcgactatt cacagcctgt cctctcattt cccaaagatc
      121 cacaatactt ggtgttgaca aaaatttatg atagctatat gtagctatcc attgaagcaa
      181 acgagctccc cctttcctct ctcaggaggc ttaccgtgag aattgatcga agatttaccc
      241 gtcgcgggca gaatccctac gaggggatca cgtttgtgaa gcgtctgtcg gagattcgga
      301 atcccgacgg gtccaccgta ttcaagctcg aaaatattga cgttcctgag gcatggtctc
      361 agcttgccgt cgacatattg gcccagaaat actttcgaaa ggccggagtt ccgcagcgcg
      421 atcagaatgg acaaccggtc atcggtccgg atggcaagcc ggtattgggc ggcgagcggg
      481 acgctcgtca agtcttcgag cgcatggccg ggtgttggac ccattggggc aagtcctatg
      541 ggtatttcaa gacgccagaa gatgccgagt cgttttacga tgaaatgtgc tacatgctgg
      601 cgcaccaaat ggccgcgccg aattctcccc agtggttcaa tacaggcctt cattatgcct
      661 atgggttatc gggaccggca caagggcatt attacgtcga tccccagact tgcgaagttg
      721 tgaaagccac caatgctttc gaacatcctc agcctcatgc gtgtttcatt caatcaattg
      781 aggatgatct ggtgaatgaa aacgggatca tggatctgtg ggtccgggag gcgcggttgt
      841 tcaagtatgg ttccgggacg gggactaatt tttcgaaact gcgtggggat ggcgaagggc
      901 tgtccggcgg cgggagatct tcaggtctta tgtctttcct gaagatcggg gatcgagccg
      961 ccggagcgat taagtccgga gggacgacgc gccgtgccgc caagatggtc tgtttggacc
     1021 tcgatcatcc ggatatcgaa gaattcatcg attggaaagt catcgaagaa caaaaagtcg
     1081 cggcgatggt gacgggctca aagatttgtg cacaacgcct caacgccgtg ctgaaagcgt
     1141 gtcatatggt cgatggccaa ggctctctca ggctggacct cgatccgaag accaatccgg
     1201 tcttgcgtga agccctgaca ttggcccgtc gggatatggt gcccgaggcg tatatccaac
     1261 gaatgttttc ctatgcgcag caaggtttca cgcactttgt gtttcacgag tatgacacga
     1321 attgggacgg gaaggcctat cagaccgtct ccggccaaaa ttcgaacaat agcgtcagga
     1381 ttcccaatga gttttttgcc gcgctcgaaa cagacggcga ttggcatctc aaacgccgaa
     1441 cgaacggcaa ggtctgtaag accgtcaagg cacgggagct gtgggaccgg atcgcctggg
     1501 cggcctggat ttgtgccgat cctgggactc agtacgatac catcatcaac gagtggcata
     1561 cgtgtccgga agacggtcgc atcaacgcat caaacccctg ttccgaatac atgtttttgg
     1621 atgatacggc ctgtaatctc gcctccctca atctcacgaa gttttacacc gctgatgggc
     1681 aattcgagct ggaaaatttc cgtcatgccg ttcggttgtg gacgatcgca ctggagatca
     1741 gcgtgttgat ggcctctttc ccgagccgat ccattgctga aaagagctat cagttcagga
     1801 cgctcgggtt gggttatgcg aatttgggga ccgtgctcat gcgacaaggg attccttatg
     1861 attcgccgaa ggctttggca atttgtggag ccatcacggc catcatgacc ggggaagcat
     1921 acgggacgtc agctgaaatg gctgccgaat tgtcgccctt cccgggatac gcaaaaaatc
     1981 gagagcacat gctgcgggtc atccgcaatc accgtcgggc tgcttatcag gcgtcttacg
     2041 tggaatatga aggtcttacg atcgctccgg cgggaatccg ccccgaacat tgtcctcccg
     2101 acatgctcct cgctgccaga cgagcgtggg accatgcgct tgaattaggg acggcgtatg
     2161 ggtaccgcaa tgcacaagtg accgtcatcg ctccaaccgg cacaattgga ttggtcatgg
     2221 actgcgatac tacggggatt gaaccggact ttgctctggt gaagtttaag aaactg
//
LOCUS       DASTWE010000298         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1494868, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000298 DASTWE010000000
VERSION     DASTWE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494868"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..>1573)
                     /locus_tag="VFT30_03225"
     CDS             complement(182..>1573)
                     /locus_tag="VFT30_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU5091666.1"
                     /translation="PMLADRLARYCRALRYGNLPDAVVHEVKRRMLDSLGCALGAWNA
                     PPCRVARLIAQTVKISQGTTVWGTGHKTLPDLATFANGGLVRYLDFNDTYLSKEPAHP
                     SDNIPAILSVGEAVHASGKRVVEAIVLAYEIQCRFCDAAALRPRGWDHVTYGPFSSAL
                     GAAKLMKLSKEQTVQAINLAGIANMALRQTRVGDLSMWKACAFSNAARNGVFAAMLAQ
                     RGMTGPSPIFEGEKGFMKLVSGPFDLAALGGETREASAIGQFKILDTYIKHYPVEYHA
                     QTAVEAAIALRNELMEAEGARAVDHVVDVEIGSYDVAIEIIGRDPEKWQPATRETADH
                     SFPYCVAVALLYGPVTLQSFSPKRVHDSAVRNLMKKVRVVQQPEFEGRYPDTMPTRIT
                     VKTEAGQTYMRQVDVPVGHPGNPMLDRDLEEKFRRLAAGRLNRPRIDRLIEFIWNLDR
                     VSDVGTLMPLLRI"
BASE COUNT          342 a          470 c          424 g          337 t
ORIGIN      
        1 cgcagcgcct gagacatacg ccatttcgta gccggctcgt tcgatctgca tcgcgaccag
       61 cgcgttgaat gcaccaggaa tggcatgagt gcgggtactc agcaattcac gcaagcgaga
      121 tgtcttcctc tgcattgctg aatctttgtc cgtgttcgtc atctaccgtc ctgttctcaa
      181 ctcagatcct caagagcggc atgagtgtgc caacgtcact aacccgatca aggttccata
      241 tgaactcgat aagccggtcg atgcgagggc gattcagccg tccagcggct aaccggcgaa
      301 atttttcttc cagatcccga tccaacatcg gatttccggg atgtcccacc ggcacatcga
      361 cctgtctcat gtaggtttgg cccgcttcag tcttgaccgt gatcctggtc ggcatggtgt
      421 cggggtagcg cccctcgaat tcgggttgct gcacaacacg gaccttcttc atcagattcc
      481 gcacggctga atcatgcact cgtttcgggc tgaatgattg cagcgtcaca ggaccgtaaa
      541 gcaatgccac tgcgacgcaa taggggaaac tatgatcagc cgtctcccgc gtggccggtt
      601 gccatttttc aggatcgcgt ccgatgattt cgatggccac gtcataactc ccgatttcaa
      661 catccacgac gtgatcgaca gcacgagctc cctccgcctc catcagttca ttgcgaagtg
      721 cgatggcagc ctcgacggca gtctgcgcgt gatactcgac cggataatgt ttaatatagg
      781 tgtccaagat cttgaactgg ccaatcgcag acgcctctct cgtttctcct cccaacgccg
      841 caagatcgaa cggtcccgaa accagtttca tgaagccctt ttcgccttcg aatataggcg
      901 acggtcctgt cataccacgc tgcgccagca tggcggcgaa cacaccgtta cgcgcggcat
      961 tcgaaaacgc gcaggctttc cacatggaca aatccccgac ccgcgtctgc cgaagggcca
     1021 tattcgcaat gccggccaga ttgattgcct gcaccgtctg ttccttggaa agtttcatga
     1081 gcttggcggc accaagggca gaggaaaacg gaccgtacgt cacgtggtcc cacccgcgtg
     1141 ggcgtagcgc cgcagcgtca cagaaccggc actgaatttc ataggcgagc acgatggctt
     1201 ctacgacgcg tttaccggac gcatggacgg cctcgccgac cgaaagaatg gctggaatat
     1261 tatcggaagg gtgcgccggc tctttcgaaa gataggtatc gttgaaatca agataccgta
     1321 ccagcccgcc gttggcgaaa gtcgcaagat cgggcagagt tttgtgaccg gtaccccaca
     1381 ccgtggtgcc ctgcgatatc ttgacggtct gggctatcag gcgggcaacc cggcagggcg
     1441 gggcattcca cgctccgagt gcgcaaccaa gactgtcgag catgcgccgt ttgacttcgt
     1501 gcacaacggc atccggtaaa ttaccgtagc gcaacgctcg gcaatatcga gcgagacgat
     1561 cagcgagcat cgg
//
LOCUS       DASTWE010000299         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1171499, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000299 DASTWE010000000
VERSION     DASTWE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171499"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1015
                     /locus_tag="VFT30_03230"
     CDS             <1..>1015
                     /locus_tag="VFT30_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5091667.1"
                     /translation="IFLQDGVDLAGSEADIVAISQTAVKALPPDVAAPMVMRLFPSQV
                     PVAVLQVESDVQTPAELYNLCIMRIRPMLVTIPGAILPHPYGGQDMQVMINVDQQKLL
                     ARHLTTEDVHQAIEKQNLVLPGGDMKIKETDWLVLTNASPLKIEEFDDIPIKRDGNAF
                     IHLRDVATVRLAGRVQTNTVLVDGKQAVIVVVMKSSEASTLDIVDGIKKMLPRIQEVV
                     PPDVKVSLLSDSSVFVRDAIADVVHEMIIAAALVGFIVLLMLGSWRPTVIVLTSIPLS
                     ILSSIIALHLLEQSINIMTLGGLALAVGILVDNAAVMIENIDTHLAMGKPLEMSIIDA
                     ANQI"
BASE COUNT          221 a          306 c          272 g          216 t
ORIGIN      
        1 catttttcta caggacggcg tggacctggc cggcagcgaa gccgacatcg tggcgatatc
       61 gcagaccgcc gttaaagcgc taccgccgga tgttgccgcg cccatggtca tgaggctgtt
      121 cccctcccaa gtgccggtag ccgtgttgca agtcgaatcc gacgtgcaga caccggcgga
      181 gctttacaat ctttgcatca tgcgaattcg acccatgctc gtcacgatac ccggtgcgat
      241 cctgccgcat ccctacggtg gccaagacat gcaggtcatg atcaatgtcg atcagcagaa
      301 attgctggcc cgccacctca ccacggagga cgtgcaccaa gctatcgaga aacaaaatct
      361 cgtgctgccc ggcggggaca tgaagatcaa ggagaccgac tggcttgttt tgacgaacgc
      421 ctctccgttg aagattgagg agtttgacga catccccatc aaacgagacg gcaatgcctt
      481 cattcaccta cgcgacgtcg ccacggtgcg ccttgccgga cgagtgcaaa cgaacaccgt
      541 gctggtcgac ggcaaacagg ccgtcatcgt cgtcgtcatg aagagcagcg aagcgtcgac
      601 cttggacatc gtggatggaa tcaaaaaaat gctcccacgc atccaggagg tcgtgcctcc
      661 ggatgtgaag gtgagtcttc tcagcgactc ctcggtgttc gtcagggacg ccatcgccga
      721 cgtcgtgcat gaaatgatca tcgccgccgc gctggtcggc ttcattgtgc tgctgatgct
      781 cggctcttgg cgtccgacgg ttatcgtcct cacttcgatc ccactctcca tcctcagctc
      841 catcatagcc ctccatttac tcgaacaatc gatcaacatt atgaccttgg gtggattggc
      901 attggcggtc ggcattctcg tcgacaacgc cgcggtcatg atcgagaata tcgacaccca
      961 tcttgccatg ggtaagccgt tagagatgtc gattatcgac gccgccaatc agatc
//
LOCUS       DASTWE010000300         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_493082, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000300 DASTWE010000000
VERSION     DASTWE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493082"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFT30_03235"
     CDS             <1..415
                     /locus_tag="VFT30_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091668.1"
                     /translation="GGSPGIPSRRRTCGLVWQRGHDIRFLGLLLFISALNDRWIILKN
                     LDYALPFFCTKPGGTLGFITKAISPALHLAVGYGFVRLRRWALFLYLMYAAYGFTNGI
                     VNLTCFGSGRIRNTLLVVIVLSTAYVLWRRRILIQ"
     gene            565..786
                     /locus_tag="VFT30_03240"
     CDS             565..786
                     /locus_tag="VFT30_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091669.1"
                     /translation="MADTTALYALRFPDGSVSLYIDELYAKDRGIDPSRLVRVEIPRE
                     MFVSGTIQDIREYVAFQLEQLEQQRTGTA"
     gene            881..>1269
                     /locus_tag="VFT30_03245"
     CDS             881..>1269
                     /locus_tag="VFT30_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091670.1"
                     /translation="MTHTLATEFLTAQKIEEVIAGLPIQGRIILGLLLIQYLDITHEE
                     ILYMVSDRPDPRCVSGKKPVTTMTQESITAMIERRNEYRRRARLRRERTWLQCVALTN
                     QARMASALATRAAALLTARGVSSETIER"
BASE COUNT          277 a          354 c          334 g          304 t
ORIGIN      
        1 gggagggagc ccaggcattc ctagtcggcg tcgaacgtgc ggattggttt ggcagcgtgg
       61 ccatgatatc cgattcctgg gtcttttact cttcatcagc gcgctgaacg atagatggat
      121 catccttaaa aacctggact atgcgcttcc ttttttctgc acgaagcctg gcggcaccct
      181 tggtttcata acgaaagcca tttcgccggc tctccatcta gccgtcgggt acggttttgt
      241 gcggctccgt cgatgggcat tgttcctcta cctcatgtac gccgcttacg gcttcacaaa
      301 cggcatcgtg aatcttacgt gttttggttc cggccgcatc aggaacaccc tactggtcgt
      361 cattgttctc tccactgcct atgtcctttg gcggcgacgg attttaatcc aatgaggctc
      421 aggtcagggt taaggttgag cgtggatttt ccggatgatg acctcaacct tgatgtcgac
      481 ctagaccgat gtcagcgcct ttgcggcctt tgacagtctg acgaagcctg tgttacctct
      541 acaatgtcat tcgccggggt actaatggct gataccactg ctctctacgc cctgcgtttt
      601 cctgatggct cggtgagcct ctatatcgat gagctctatg ctaaagacag agggattgat
      661 ccttcccggc ttgtgcgggt agaaatccca cgagaaatgt tcgtcagcgg caccatacag
      721 gacattcgtg aatatgtggc gttccaatta gagcaactag aacaacaacg aaccggaaca
      781 gcgtaacgca cgttgagttg ataccgaata gccgaaaggc cgatcagacg cggaggtttg
      841 ctctcaccgg actccgccct aaacacttca ttcaattatc atgacacaca ccctcgcgac
      901 cgagttcctt accgcccaaa agatcgaaga agtgatcgcc ggtttaccga tccaaggccg
      961 cattatctta gggctcctgc tgattcagta cctggatatc actcatgaag aaatccttta
     1021 catggtgtcg gacaggccag acccgcgatg cgtgtccggc aagaaacctg tcacgacgat
     1081 gacacaagaa tcgattacgg cgatgatcga gcgccggaat gaataccggc ggcgcgcgcg
     1141 gcttcgccga gaacgaacat ggcttcagtg cgtcgcgctg accaatcagg ctcgcatggc
     1201 gagcgcgctg gccacccggg ctgccgcact gctgactgcg cgtggagtgt cctcagaaac
     1261 tatcgagcg
//
LOCUS       DASTWE010000301         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1074832, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000301 DASTWE010000000
VERSION     DASTWE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074832"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VFT30_03250"
     CDS             complement(<1..1197)
                     /locus_tag="VFT30_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5091671.1"
                     /translation="MNNLGRTTPMILIIIVGLMFLGYRFSENQQEAAALQAGNLESAV
                     TPVALTQAKLSEPTDTITLPGNIVGWNEAPIYARVTGYVKEWHKDYGHDVKKGDVLAE
                     ITTPDLDAEYRQAVADLESERARNALADLTAKRYVAMRQNQALSEQAISVQIAEAKAQ
                     AAKVAAAEQKVKNIAAFIGFKQITAPFDGVVIQRNISIGDLVSKEGNLNTPNAKNNLF
                     TVAVVDKLRLFVSVPESFGAFLSPGLTADVTVPQFPNRHFTFKFLTVSKGFDVSTRTA
                     VAVFTIDNKDHVLWPGSYATVRLTAPVDLGAMTIPTSAMVFQEHGTQVAVVGEDDRLR
                     FKPITVAKILDNIVEVTEGISESDRIVNNPSAALLEGDQVRIVTSAPGYNIVTREAPT
                     PEARAQK"
BASE COUNT          283 a          347 c          337 g          302 t
ORIGIN      
        1 cttctgcgcc cgggcctcgg gagttggagc ttcacgagtc acaatgttat agcctggcgc
       61 ggatgtgacg atgcgcacct gatctccttc gagcaaggcg gcactcggat tgttcacaat
      121 acggtcgctc tcagaaatgc cctctgtcac ttcgacgatg ttgtcgagaa tcttggccac
      181 agtaatcggc ttaaagcgaa gccgatcgtc ctcacccacg acggccacct gcgtgccatg
      241 ctcctgaaac accatcgcgc tcgttggaat cgtcatcgcc ccgagatcca caggagcggt
      301 cagacgaacg gtggcgtagg agcctggcca gagtacgtga tccttattat caatggtaaa
      361 gaccgccacg gcggtgcgtg tgctcacatc aaaccccttc gacacggtca gaaacttgaa
      421 agtgaaatga cgatttggga attgcggtac ggtcacgtcg gccgtcaggc ccggactgag
      481 aaaggcccca aaggactccg ggacgctcac aaagagtcgc aacttatcga ccacggccac
      541 ggtaaagagg ttgtttttcg cattgggggt gttgagattg ccctccttac tgaccaaatc
      601 accgatactg atattgcgct gaatcacgac tccgtcgaaa ggagccgtga tctgtttaaa
      661 tccgatgaag gccgcaatat tcttaacctt ttgctccgcc gccgcgactt tcgccgcctg
      721 ggctttcgcc tcggcgattt gtactgagat agcttgctcg gagagcgcct ggttttgtct
      781 catcgccaca tagcgtttgg cggtcaggtc ggccagcgca ttcctggcac gttcggattc
      841 tagatctgca acagcctgtc ggtattcggc atcgagatca ggcgttgtaa tttccgcgag
      901 gacatcgcct ttcttcacat catggccata gtccttatgc cactccttca catagcctgt
      961 gacacgcgca tagatgggag cctcgttcca gccgacaata ttgccgggga gtgtaatggt
     1021 gtcagttggc tctgatagct tggcttgcgt gagggccact ggagttacgg cgctttccaa
     1081 atttcccgcc tgcaatgcgg ccgcctcctg ctggttctcg ctaaaccgat aaccgagaaa
     1141 catgagtccc acgatgataa ttaaaatcat aggggttgtt ctgcctaagt tattcatatt
     1201 agacacactc cttttggaga acagttcgtc ggtgatagat gatggcatag acacagggaa
     1261 cgaaaaaca
//
LOCUS       DASTWE010000302         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1430340, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000302 DASTWE010000000
VERSION     DASTWE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430340"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFT30_03255"
     CDS             complement(<1..323)
                     /locus_tag="VFT30_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091672.1"
                     /translation="MRGLAAHEVLEIWDQGEGLSPSGRAALLLAVACPDYPRERVESL
                     SIGERDTLLGHIRARTFGGTVESVVTCPACGAMVEVAVDVRTIFPEVTQIASPEAIPR
                     LPVEIA"
     gene            complement(330..848)
                     /locus_tag="VFT30_03260"
     CDS             complement(330..848)
                     /locus_tag="VFT30_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU5091673.1"
                     /translation="MAQFSVNVQRFDPYKNFKFRVKWDGRYVAGVSKVGALKRTTEVV
                     EHREGGDPSTGRKSPGRTKYEAITLERGVTHDLEFEKWANKVWNFGAGLGREVSLKDF
                     RKDIIIEMYNEAGQLAFAYKVFRCWVSEFQAQPDLDANANAIAIQTIKLENEGWERDR
                     DVPEPIELSLST"
     gene            complement(861..2447)
                     /locus_tag="VFT30_03265"
     CDS             complement(861..2447)
                     /locus_tag="VFT30_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEU5091674.1"
                     /translation="MPTSPTYPGVYIEEIPSGVRTITGVATSIAAFIGRALRGPTNEP
                     ITINSFGDFERIFGGLWGGSSLGFAVRDFYLNGGGQAIIVRLYHAESGTGAKPAQAKL
                     TADSLGLEAANSGVWGNKLRVRVDHNTRPVQSGEAADSLFNLTVRDGETGAIESFRNI
                     SITAGHPRQVDKVLLNESQLVRLTGNLPTARPAKSNDPAAGKDPWEDNQPATNYKVVA
                     AGEASDGAALTKADDFTPTNAETNKKGLYALAKADLFNLLCIPPYLAGGNVDTGLISE
                     AAVYCERRRAMLLVDPPIEWTTKDQAKSGVAGLGTTSKNAALYFPRLRQPHALKENQL
                     EEFVPCGAVAGIFARTDAQRGVWKAPAGLEATLVGVPGLSVLLNDQENGELNPMGINC
                     LRVMAPAGPLVWGARTLQGDDRFASEWKYVSVRRLALFIEESLYRGTQWVVFEPNDAP
                     LWAQIRLNIGAFMHNLFRQGAFQGQTPQEAYFVKCDKETTTQADINLGVVNIMIGIAP
                     VKPAEFVIIKLQQMAGQIQA"
     gene            complement(2510..2878)
                     /locus_tag="VFT30_03270"
     CDS             complement(2510..2878)
                     /locus_tag="VFT30_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091675.1"
                     /translation="MDKIRVLLANHPVMLPDAIRRLVEDQDDMEVVGDCRGPMKILQE
                     TGRLSADAVILAREQAEDPGLCSQLLAVYPDLVILSVTPDLTTVATLRLSSHRQEFAD
                     LHQEDIVQPLRTAIRMSGQR"
     gene            complement(3067..>3445)
                     /locus_tag="VFT30_03275"
     CDS             complement(3067..>3445)
                     /locus_tag="VFT30_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5091676.1"
                     /translation="GEGAAGYLLIDSSLDDLIGNIKAVMNGETLCSPRVASLAFDRVS
                     ALTRQIESRQVVDKARLTKRETEIVRLIDEGLTNKEIAVHLHIEVSTVKNHVHNILDK
                     LHLHNRYSAVKHIKAQASLTGRC"
BASE COUNT          683 a         1037 c          944 g          781 t
ORIGIN      
        1 gcgatttcca caggaagccg gggaattgct tcaggtgatg cgatctgtgt tacctcagga
       61 aagatcgtgc gcacatcgac ggccacttcc accatcgctc cgcaggcggg acaggtgacg
      121 acgctctcga ctgttcctcc aaacgtgcgt gcgcggatat ggcccagcag agtatctcgc
      181 tccccgatgc tgagcgattc cacacgctca cgcgggtagt caggacaggc gaccgccagc
      241 agaagcgctg cacgcccaga gggtgacagg ccttcgccct gatcccagat ttcgagcacc
      301 tcgtgtgccg cgagtccgcg catgatcctc tatgtcgaca gggacagttc tatcggttcg
      361 ggaacgtccc gatcccgttc ccaaccctcg ttctcaagct tgatggtctg gatcgctatg
      421 gcattcgcat tcgcgtccag atcaggttga gcctgaaact ccgacaccca gcagcgaaat
      481 accttgtagg caaaggcgag ttggcctgct tcgttgtaca tttcaatgat gatgtctttg
      541 cggaaatcct tgagcgacac ttcccgcccc aagccggcgc cgaaattcca caccttgttg
      601 gcccacttct cgaactccaa atcgtgtgtg acaccccgct ccaaggtgat ggcctcgtac
      661 ttcgttcgtc ccggcgactt gcggccggtg ctggggtcac cgccttcccg atgctccacg
      721 acttccgtcg tgcgcttgag cgctccgacc ttgctgacgc ccgcgacgta ccggccgtcc
      781 catttgacgc gaaacttgaa atttttgtat ggatcaaacc gctgcacgtt gacgctgaac
      841 tgggccatag ctggtcctcc ttatgcctga atctgaccgg ccatttgctg gagcttgatg
      901 atcacgaact ccgccggctt gacgggggcg atccctatca tgatgttgac aactcccaga
      961 ttaatgtccg cctgggttgt cgtctctttg tcgcatttca cgaagtatgc ttcttgcggc
     1021 gtttgtccct gaaacgcgcc ctgcctgaac aggttgtgca tgaaggcgcc gatgttgagc
     1081 cggatctgcg cccagagcgg cgcatcgttc ggctcgaaca ccacccactg ggtgccgcga
     1141 tagagactct cctcgatgaa gagcgcaaga cggcgaaccg agacatactt ccactccgat
     1201 gcaaatcgat cgtcgccttg aagagtgcgt gcgccccaca ccagcggccc ggcaggcgcc
     1261 atcacacgca aacaattgat ccccatcgga ttcaactcgc cgttctcctg atcgttcagc
     1321 aacacactga ggcccggcac gccgaccagc gtcgcctcca ggcctgccgg agccttccac
     1381 acgcctcgtt gcgcatcggt tctggcaaag atcccggcga cggcgccgca agggacgaac
     1441 tcctcaagct gattctcttt gagcgcatga ggctgacgga gacgtggaaa gtaaagcgcg
     1501 gcgttcttgc tggtcgtgcc taaaccagcc acaccggatt tggcttggtc cttcgttgtc
     1561 cattcgatcg gaggatccac cagcaacatg gcccgtctgc gttcacagta gaccgccgct
     1621 tccgagatca gaccagtgtc cacattgccg ccggcgagat agggaggaat gcacaacaga
     1681 ttgaagagat ccgccttcgc cagggcgtac aacccttttt tgttagtttc agcgttcgtg
     1741 ggcgtgaaat cgtcggcttt ggtcagcgct gcgccgtccg aggcctctcc ggcggctact
     1801 actttataat tcgtcgccgg ttgattatct tcccacgggt ctttgcctgc cgccggatca
     1861 ttactcttcg cgggacgtgc ggtgggcagg ttgccggtca aacgcaccag ctgcgattcg
     1921 ttgagcagga ccttatcgac ctggcgcgga tggcccgcag taatcgatat attgcgaaaa
     1981 ctttcaatcg caccggtttc cccgtctcgc acggtgaggt tgaagaggct gtcggcagcc
     2041 tcgcccgatt gcaccggccg tgtgttgtga tccacacgca cgcggagttt gttcccccag
     2101 acgccggaat tggcagcctc caggccgaga gaatccgccg tgagtttcgc ttgcgccggt
     2161 ttggcccccg tgccgctttc ggcatggtag agacggacga tgatcgcctg tcccccgccg
     2221 ttcaaataga agtctcgaac ggcgaaaccc agactgctgc ccccccacaa gccgccgaag
     2281 atccgttcaa aatccccaaa gctgttgatg gtgatcggct cgttggtcgg ccctctgagg
     2341 gctcgcccga tgaatgcggc gatggaggtg gcaacaccgg tgatggtgcg aacgccactc
     2401 ggaatctcct cgatgtaaac acctggatat gtcggtgatg tcggcattgg cttcctccgc
     2461 tagactgttg gccttatcct tcaatgcact gcactggttc aaccatgctt tagcgttggc
     2521 cgctcattcg gatcgctgtg cgaagcggct gtacaatatc ttcttgatgg agatccgcaa
     2581 actcttgccg gtgcgaggag agccgcagtg tcgcgaccgt cgtcaggtcc ggagtaacac
     2641 tcagaatgac taaatccggg tacacagcca gcagctggct gcaaagacca ggatcttccg
     2701 cctgttcccg tgcaagaatg accgcgtcgg cgctcagtct tccggtctct tggaggatct
     2761 tcatcggccc tcggcaatcc cctaccactt ccatgtcgtc ctggtcttcc accagtcttc
     2821 gaatggcatc aggcagcatc acgggatgat tcgccaggag cactctgatc ttgtccaaca
     2881 gcccccctcg tcatgccagg ccgacacatc cttagtcaca acgttgcaag atgcctatgg
     2941 atctttagga tgaatttgga tctagacatg ggagtgaagg atctagatct tcctccaagc
     3001 gcagttggca gcagggacga tgcagagagc ttctagcccc tgagcgacag aacaaattcc
     3061 ggaaggctag cagcggcccg tgaggctcgc ctgggctttg atatgtttta ccgctgaata
     3121 gcggttgtgt agatggagtt tatcgaggat attgtggaca tgattcttga cggtagaaac
     3181 ttcaatgtgc agatgtaccg cgatctcctt gttcgtcaaa ccctcgtcga tcagtctgac
     3241 gatttccgtt tcacgttttg tgagacgagc tttatcgacg acttgacgac tttcgatctg
     3301 acgagtgagc gcggacactc gatcgaaggc taaactcgca actcgtggag agcacagtgt
     3361 ttccccgttc atgacagctt tgatgttgcc gatgagatcg tcgagagatg agtctatcaa
     3421 gagatacccg gccgctcctt cgcct
//
LOCUS       DASTWE010000303         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_977917, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000303 DASTWE010000000
VERSION     DASTWE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977917"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFT30_03280"
     CDS             <1..912
                     /locus_tag="VFT30_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091677.1"
                     /translation="ETVATLGGEFPISPDRQAREVRCPHVVGRAVNGGSHAADITDCS
                     PRSDRTQVLGTLIAQDAAIFLFAFSLIGILESFNPSVSAPFPHTNIVLRMSRILLVPL
                     PLALSLLVLMFSGLERPILAAPGGMTEVLPVQKSAPVAVVIETFGSEAELRRQMRIKN
                     NVAELSTGTIRFSEAPVRSFGFIGSQALGMALVMQSPDLEFERVASTSNTYEIHKVAD
                     GSGLLVGFMDRETASKISPTERPKNLRIVLYSNPVEKAPIIVAVPLIKVMVDRMPIRL
                     DPQKSDGPVMLDMDLLTTVNRKSTVGQ"
     gene            1153..>2015
                     /locus_tag="VFT30_03285"
     CDS             1153..>2015
                     /locus_tag="VFT30_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091678.1"
                     /translation="MSHRHCTSTRVLSGLAAIALSIAPSAVFAETPSHVAMNHQLPGW
                     AEQLKGQTIIENTMEGQPERTTMVERQHQRIMQQMEQDKAAQRTGGYYNDLTMMHQYG
                     AGNQDVLLMSNSGTEPVSTSGGRCPTSAPVRKYDISAINVEISLNMWLDFYPGYMYVL
                     TENLEKVREEEAANRTAREKEGYDPGAVKNGLQNQWIQPLVIRGNQGDCVKMTLRNQL
                     EGGEDVSLNIHGSSMIVASTGKPATTTNPDSIAAKGKSVDLEWYIPPTQQEGGRQFHS
                     YSNDRELTVMG"
BASE COUNT          459 a          518 c          552 g          486 t
ORIGIN      
        1 gagaccgttg ccacgctggg tggggagttt cccatctcgc cggatcgcca ggctcgtgag
       61 gtacgatgcc cccacgtggt cggccgggct gtcaatggtg gatcacatgc ggcggatatc
      121 acggactgct cgcccagatc agacaggacc caagttctgg ggactcttat tgcgcaggat
      181 gcggccattt tcctatttgc tttttcgttg attggcatcc tagaatcctt caatccatcg
      241 gtaagtgccc ctttccccca tacaaatatc gttttacgca tgagccgtat cctccttgtt
      301 ccacttcctc ttgccctatc tctccttgtt ttgatgttct ctggtcttga gaggcctatc
      361 ctggctgcac cgggaggaat gacagaggtc cttcccgttc agaaatccgc accagtagcg
      421 gttgtgattg aaacgttcgg aagtgaagct gagcttcggc gtcagatgcg tatcaagaat
      481 aatgtcgccg aactctccac cgggaccatc cggttttctg aagcgccggt aaggagcttc
      541 ggtttcattg gatcacaggc cctcggaatg gccttggtca tgcaaagtcc tgatcttgag
      601 tttgagcgag tggcctcaac ttctaatact tatgaaatcc ataaggtcgc agacggcagc
      661 ggattgctgg ttggattcat ggacagggaa acggcgtcta agatttctcc caccgaacgg
      721 cccaagaatc tgcgcattgt gctctactcc aatcccgtgg aaaaggcgcc catcatcgtc
      781 gccgttcctt taattaaggt gatggtagac cggatgccga ttaggctcga tccccaaaaa
      841 tcggatggcc ctgtgatgct cgatatggat ttgctgacta cggtgaaccg gaagtcaacc
      901 gttgggcaat agcattgtga aaatatgatg aatggcagtc agtttcgaag tattgacagt
      961 ctattcgaag ttgagtatct ttcggcacca tctatccacc ttgtagtgta gtgaagtgaa
     1021 cgtacatatc tttgggtcag ggttggttat taccgagcta ttttgtcttc ccatttttca
     1081 cattgttgat cgagttggtt gagtagcctc cgctgctcac ttacatttat gtttatgaag
     1141 gagggtcggt tcatgtctca ccgtcattgt acatccaccc gagtcttgag tggactcgct
     1201 gcgatcgcgc tgagtattgc gccatcggcc gtgtttgctg agactccatc gcatgtggcg
     1261 atgaaccatc agctgccggg ttgggccgag cagctgaagg gacaaacgat catcgagaat
     1321 accatggaag gccaaccgga acgcactacc atggtcgaac ggcagcacca gcgtatcatg
     1381 caacaaatgg agcaagataa ggctgcgcaa cgtaccggcg ggtactacaa cgatctgacc
     1441 atgatgcatc agtatggtgc cggaaatcag gacgtgctgc tcatgtcgaa ttccggcaca
     1501 gagccggtat cgacgtcggg tgggcgctgt ccgacgtccg cgccggtccg aaaatacgac
     1561 atctcggcga tcaacgtcga gatttcgctc aacatgtggc tcgatttcta tcccggttat
     1621 atgtacgtcc tcactgagaa cctcgaaaaa gtgcgggaag aggaagccgc caatcggacg
     1681 gcccgcgaga aggaaggcta tgatcccgga gccgtcaaga acggattgca gaatcagtgg
     1741 attcagccac tggtcatccg aggtaatcag ggcgattgcg tcaagatgac gcttcgcaat
     1801 caactggaag gcggcgagga tgtcagcctc aatattcacg gctccagcat gatcgtcgcc
     1861 tctacgggca agccggcgac gacgacgaat ccggacagta tcgcagctaa aggtaagtct
     1921 gtggatctgg agtggtatat tccaccgact cagcaagagg gcggccggca gttccactcg
     1981 tacagcaacg atcgcgagct gacagtcatg ggcat
//
LOCUS       DASTWE010000304         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_913247, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000304 DASTWE010000000
VERSION     DASTWE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913247"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFT30_03290"
     CDS             <1..792
                     /locus_tag="VFT30_03290"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEU5091679.1"
                     /translation="SVLESAKQLVGRVHGVNVTDNTAAVVRAGSLPVCRLLYELGHDP
                     VMQLTCRDRNRIAMQSDLMGAHMLGIRNILCLTGDYPTVGDHKEAKPVYDLDSVQVMQ
                     LVQGLNKGHDMAGHKLDGSTAFTIGAAVTPEADPLGPMLAKFEVKVKAGAQFFQTQAV
                     YHPEQFASFMKAVRPYKVKVLAGILVLRNHKMAEFMNGNIPGISVPSEMIDELKAAGE
                     KAEDVGVEIAVRTIKAVRPHCDGVHIMAIKATHRLAEIITKAGLN"
     gene            792..>1558
                     /gene="panB"
                     /locus_tag="VFT30_03295"
     CDS             792..>1558
                     /gene="panB"
                     /locus_tag="VFT30_03295"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5091680.1"
                     /translation="MTILDFHQFKRDRKKLAVVTAYDALFARIVEQAGIRVILVGDSL
                     GMVVQGKSNTLTVTMDDMLYHTKLVAGVVQRALVIADMPFMSYQASTEEALRNAGRLL
                     QAGAAAVKLEGGAVMVDRIKAMTNVGIPVMGHLGMTPQSVYALGGYRVQGKADDQASR
                     LLDDAKALEAAGAFAVVLEAVPAALAKKITQSLSISTIGIGAGPHCDGQVLVLYDLLG
                     LFDAFIPKFVKTYAHLKADTLQALSRYKEEVEQGKFPS"
BASE COUNT          356 a          403 c          462 g          337 t
ORIGIN      
        1 tccgttctcg agagtgccaa acagcttgtc gggcgtgtac acggtgtgaa cgtcaccgac
       61 aataccgccg ccgtggttcg tgccggctca cttccggtct gccgcctact ctatgagtta
      121 gggcacgacc ccgtgatgca gttgacctgt cgtgatcgca atcggatcgc catgcagtcg
      181 gatcttatgg gcgcgcacat gcttgggatt cgaaatattc tttgccttac aggcgattat
      241 ccgacggtcg gtgatcataa ggaggccaag ccggtgtatg atctcgattc agtccaagtc
      301 atgcagctcg tgcaaggatt gaacaagggt catgacatgg ccgggcacaa gctggacggc
      361 tcgacggcat ttacgatcgg tgccgccgtc acacctgagg ccgatccact cggaccaatg
      421 ctcgccaagt ttgaagtgaa agtgaaagcg ggtgctcaat ttttccagac gcaagcggtt
      481 taccatccag agcagttcgc gagcttcatg aaggcggtcc gaccctataa ggtaaaggtt
      541 cttgccggta tccttgtgct gcgcaaccac aagatggcgg agttcatgaa cgggaatatt
      601 cctggcatct cggttccaag cgagatgatc gacgagctca aagccgccgg tgagaaagca
      661 gaagacgtgg gagtcgaaat tgccgtacgg acgatcaagg cggttcggcc gcactgcgac
      721 ggcgttcata tcatggcgat taaagcaacg catcggttgg ccgagatcat caccaaagct
      781 ggattgaact gatgaccatc ctcgatttcc accagttcaa acgagacagg aagaaactag
      841 ccgtcgtgac ggcctatgat gcgctgttcg cacgtatcgt cgagcaagcg gggattcggg
      901 tgattctggt cggggattcg ctgggcatgg tcgtacaggg gaaatccaat acgcttacgg
      961 tcacgatgga tgacatgctg tatcacacca agctggtcgc cggtgtggtg cagcgagctc
     1021 tggtgattgc ggatatgccg tttatgtcct atcaggccag caccgaggag gccctgcgca
     1081 atgcgggccg attgctgcaa gcaggtgccg ctgcggtgaa gctggaggga ggggcggtga
     1141 tggtagaccg aatcaaggcc atgacgaatg ttgggattcc tgtcatgggc cacctgggta
     1201 tgacaccgca atcagtctat gcgctgggcg gatacagggt ccaaggcaaa gccgacgatc
     1261 aagcctccag attacttgac gatgcaaaag cccttgaagc tgctggggca ttcgctgttg
     1321 tgctggaagc ggtgccggca gccctggcca agaagatcac ccaatccctt tcaatctcta
     1381 ccatcgggat tggagcggga cctcactgtg atggccaagt gctggtgctc tatgatcttc
     1441 ttggactctt tgatgcgttc atcccgaaat tcgtgaagac ctacgctcac ctcaaagccg
     1501 ataccctcca agcactaagt cgttataagg aagaagtgga gcaaggaaag ttcccgtc
//
LOCUS       DASTWE010000305         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_622257, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000305 DASTWE010000000
VERSION     DASTWE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622257"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..558
                     /gene="trxA"
                     /locus_tag="VFT30_03300"
     CDS             211..558
                     /gene="trxA"
                     /locus_tag="VFT30_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5091681.1"
                     /translation="MAGDALKVEDSSWDAEVMKAAELVMVDFWAVWCGPCQMVAPIVD
                     ELAKEYAGKLKVRKLNTDENPEVAGRYQVMSIPTILFFKNGQPVEKLVGARPKRQFKE
                     VIDSLLAQHAGTA"
     gene            756..>1695
                     /locus_tag="VFT30_03305"
     CDS             756..>1695
                     /locus_tag="VFT30_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5091682.1"
                     /translation="MLTELRITNFAVIERLSLIIDSGFTVLTGETGTGKSLLIDAVAL
                     LIGGRASSDQIRFGEDEAQLEASFEIPLTHPLLQRLRVREVLGPQDSQLIIRRIIARS
                     GKNRVYLNGVLSPVHVLEEFAGTLIDIHGQHDQQSLLSNSAQLEVLDAFGRLLDLRSQ
                     YRSTHREWLRFREERAELAARLQQQTQQEDLLRFQQQELDEAACRIGEEELLQAERHR
                     LGSSRRLVELASEAQERIQGDVHGILANLVLMERALGELSQIDPEMESTVRFASEAKV
                     LLKEVADSLRGYAESLDADPMRLTAIEDRLAVIQKMK"
BASE COUNT          365 a          420 c          496 g          414 t
ORIGIN      
        1 tgagcccgct tctgtggggt gagcgtggtc tgccgtgggc gacgctggga gggccctatg
       61 gtattcctcc gcagacctcc gagacagttg gtgtgccgcc cggcgttcaa acccgacagt
      121 gatccgtgtt tgacactaaa aacagtggtg gggtacgatg ccctgggcaa tcgcgcataa
      181 atgatctcaa ttctgaagga gggtgcagac gtggctggtg atgccttgaa agttgaagat
      241 tccagttggg atgccgaagt tatgaaggct gccgaactcg tcatggttga tttttgggct
      301 gtgtggtgcg gtccttgcca aatggtggct cccatcgtcg acgagttggc aaaagaatac
      361 gccgggaagc tgaaggttcg aaaactgaat accgacgaaa atccagaggt cgcgggtcgt
      421 taccaggtga tgagcatccc tactattctt ttctttaaga atggccagcc ggttgaaaag
      481 ctggtgggtg ccagaccaaa gcgccagttc aaagaagtga ttgactcact gctcgctcag
      541 catgcaggca ctgcctagcg cgacactgaa agagccttca acctcgtcct tgcgttgctc
      601 agtagcgctc agcgtgtcaa agcgtaggct tcaacgtcgt ttttgttgtg gcattgttgg
      661 gcagtcattg agcgatacgt tcaatcggca ccagcgattg ttcaatgcat ctatgtgtct
      721 tctgcagcta gttgggtctc gccactgttg cagctatgct cactgagctg cgtatcacaa
      781 actttgccgt gatcgaacgc ctgagtctga tcattgactc gggatttacc gtgttgaccg
      841 gagaaacagg gaccgggaag tcgttgttga tcgatgcggt ggcccttctt attggcggac
      901 gggcctcaag cgatcagatt cgattcggcg aagacgaagc ccagcttgaa gcgtcgttcg
      961 agattccgct cacccatcct ctccttcaac ggctgcgggt ccgagaggtc cttggaccgc
     1021 aggattctca actcatcatt cgacgcatca ttgctcgttc aggaaaaaac cgggtttacc
     1081 ttaatggagt cttaagcccg gtccatgtgc tggaagagtt cgccggtact ctcattgata
     1141 tccatggcca acacgaccag caatcgctct tgtccaactc cgcacagctc gaggttctgg
     1201 atgccttcgg acgtctgctg gatctgcggt ctcagtatcg atccacccat cgtgagtggc
     1261 tacggttccg tgaagaacgc gctgagcttg ccgctaggct tcaacagcag acccagcagg
     1321 aagacttgtt gcgttttcaa cagcaggaat tggatgaagc tgcctgccgt atcggggagg
     1381 aagagttgct gcaagccgaa cgccatcggt tggggtcgtc gcggcgtctg gttgagttgg
     1441 catcggaagc tcaggaacga attcagggtg atgttcacgg catcttggcg aatctggtgt
     1501 tgatggagcg tgcgttagga gaactatctc agatcgaccc tgagatggaa agcaccgttc
     1561 gatttgcgtc cgaggccaaa gtgctcctga aagaagtcgc cgattctctt cgcggttatg
     1621 ctgagagctt ggatgccgat cctatgcgac tcactgcgat cgaggatcgt ttggctgtta
     1681 tccaaaagat gaaaa
//
LOCUS       DASTWE010000306         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_234827, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000306 DASTWE010000000
VERSION     DASTWE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234827"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFT30_03310"
     CDS             <1..239
                     /locus_tag="VFT30_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091683.1"
                     /translation="MQNFVTRNQQGAWRPNLPQWFGAIGGSAIAVYGWYPDSNSHGNE
                     ILRTAAFQLAVPIAFNAIVEFGPEIKRGFLFWRR"
     gene            complement(401..>1197)
                     /locus_tag="VFT30_03315"
     CDS             complement(401..>1197)
                     /locus_tag="VFT30_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEU5091684.1"
                     /translation="KHRSYPGRPRIGAEVEALIVQMARDNSGWGYDRIAGTLAHLGHD
                     VSDQTVGNVLHRHRIAPAPKRSQTTAWKDFLASHMAVLAGIDFFTAEVLTWRGPATYY
                     VLFVIQLETRRVTLAGITRHPSEEWMQQIARNLTDTESGALRDQLYLLHDRDTKFCAR
                     FRSILRDGGVEPLKLPPRSPNLNAFAERWVRSVKEECLSKLILFGENSLRRALNEYVA
                     HFHAERPHQGKGNVLLFPEIERARRGDRVECKQRLGGLLRYYARAS"
BASE COUNT          243 a          336 c          344 g          274 t
ORIGIN      
        1 ccatgcagaa tttcgtcacc aggaatcagc agggtgcgtg gaggcctaac ctgccacaat
       61 ggtttggtgc tattggtggc tctgcaatcg cagtctacgg ttggtatccg gatagcaata
      121 gccacggtaa cgagatactg agaaccgctg ctttccagct cgcagttcct atcgctttta
      181 atgcgatagt cgaatttggt cccgagatca agagaggctt cctattctgg cggcgttgat
      241 gagggtgacc ccttatctct ttcccaacgt ctgacagtct gaagttgact tcccatttct
      301 ttacagctat ctctatgacg cccgtgacgt ctgtccttct ccagtgctag atgtgtctct
      361 ctcgccggac ggccaggcaa tcgtcgttag gtcaaattat tcatgaggcg cgagcataat
      421 accggaggag gcctcccaga cgttgcttgc actcaacacg atcaccccga cgggctcgtt
      481 caatctcagg gaacaacagg acgtttcctt ttccctgatg cggccgctcg gcgtgaaagt
      541 gcgcaacgta ctcgttgagc gcgcgtcgga gcgagttctc cccgaacaga atcaactttg
      601 acagacattc ttcttttact gagcgcaccc atctttcggc gaacgcgttc aggttgggag
      661 aacgtggcgg gagcttcagc ggttcgaccc cgccatcgcg aaggatggag cggaatcggg
      721 cgcagaactt cgtgtcacgg tcatgcagaa ggtagagctg gtcgcgaagg gctccggatt
      781 cggtatcggt cagattccgg gcgatctgct gcatccattc ctcgctcgga tggcgggtga
      841 tgccggctag cgtcacacgt ctggtctcca gctggattac aaacagaacg taatacgttg
      901 ccggaccgcg ccaggtgagc acctcggcgg taaagaagtc tatacctgcc agaaccgcca
      961 tgtgcgaggc gaggaagtct ttccaggcgg tggtctggct tcgtttcgga gccggtgcga
     1021 tccggtggcg gtgcagaaca ttgccgaccg tctgatccga gacatcgtgg ccgagatggg
     1081 ctaaagtacc ggcaatccgg tcatagcccc acccggagtt gtcccgagcc atctgcacaa
     1141 tcagcgcctc cacttcggcg ccgatcctcg gccgaccggg atatgaacga tgtttgg
//
LOCUS       DASTWE010000307         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_880907, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000307 DASTWE010000000
VERSION     DASTWE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880907"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..>1282)
                     /locus_tag="VFT30_03320"
     CDS             complement(282..>1282)
                     /locus_tag="VFT30_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU5091685.1"
                     /translation="YQLLELAGFAGQANENIAAYKPYFANIDDLGRLLPAIEEGGVGI
                     GSSPLDTVSTGSTIKTTVNGCPALKPNLHKVAFFLHSTANNNPFGSISVFHDADKKLD
                     PNLEDFFERYGGPRASLSTNAEGERLTVPDVQANRVVLVAGDSIAFGAMIDDSETIAS
                     KLQANDATHRYVNVGVPGVDAKDVICRLEDFSKRYKGRIDELIYVYCENDFIPGQPYG
                     TPAEVIGWLTDFVEREKITKVAVVFAPLIYTVAPEITRIEGTKGIKNPRREKERAELI
                     DLVKATGYRFVDIGALAREEEDKQKTHFGVLSFFVDTGHPSPLGTTRITEALETQD"
BASE COUNT          271 a          347 c          342 g          322 t
ORIGIN      
        1 ggactctgac tccgccagtc taggttcgaa tcctagttcc ccagccactt gatttcctta
       61 cacttccctc gtttgtcatc tggaacattt caggtcagtc ggacgcttgg ttgggagaaa
      121 tcgttctcct ttcgagcttt cggattgcag attcgccgag tgcgggaaag cggtgcaggt
      181 agtgtgcatc gaggatcgaa cgcgtcctac gcaatttggt cgatggatgt ccttcgccca
      241 ccgcgttcct gtcgccgcag cgcgattttc gatcaaagtg atcaatcctg cgtttccaga
      301 gcctccgtaa tgcgcgtcgt gcctaaaggt gaaggatgac cagtgtcgac gaagaacgaa
      361 aggacgccga aatgagtttt ctgcttgtcc tcttcctctc gagccaatgc gccgatatcg
      421 acaaagcggt agccggtcgc tttgaccagg tcaatcaatt cggcgcgctc tttctctcgc
      481 cttggattct tgattccttt cgttccctca attcgcgtga tctccggtgc gaccgtataa
      541 atcagtggcg caaaaaccac ggctaccttg gtgatcttct cccgctcgac aaaatccgtc
      601 aaccatccga tcacctctgc cggtgtgcca tagggctggc ccgggatgaa gtcgttctca
      661 caatagacat agatcagttc atcgatgcgc cccttatatc gctttgaaaa atcttcgagc
      721 cgacagatta catcttttgc atcgacaccg ggaacaccaa catttacgta acggtgtgtc
      781 gcgtcatttg cctgcagctt cgacgcaata gtttcggagt catcgatcat ggcgccgaag
      841 gcgatcgaat cacctgcaac caagaccacg cgattggctt gtacatcggg aaccgtcagg
      901 cgctcaccct cggcatttgt ggagagtgag gcacgtggtc cgccgtagcg ctcaaagaaa
      961 tcctccaggt tcgggtcgag cttcttatcc gcatcgtgga agacgctgat cgatccgaag
     1021 gggttgttgt ttgcggtcga atggaggaaa aaggcgacct tatgcaggtt tggcttgagg
     1081 gcgggacagc cattcacggt tgtttttatg gttgaccctg tagacacggt atcgagcgga
     1141 gagcttccga taccaacgcc accttcctcg atcgcaggga gcaggcgccc gagatcgtca
     1201 atattggcaa agtaaggctt gtatgctgcg atgttttcgt tggcttggcc agcaaacccc
     1261 gcgagttcca ggagttgata gg
//
LOCUS       DASTWE010000308         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_525502, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000308 DASTWE010000000
VERSION     DASTWE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525502"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFT30_03325"
     CDS             complement(<1..571)
                     /locus_tag="VFT30_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5091686.1"
                     /translation="MKEDSSQSDLVEGITDTFSRPLKNLRLSVTDRCNLRCKYCMPEE
                     EYVWLPREDILSFEEMTTLAGYFTDLGVDKVRLTGGEPLLRRDLARLVRLLLQNRRIT
                     EVALTTNGVLLAEYVQELYDAGLNRITVSLDTLRPERFRRLTGRDEFARVLEGIESVG
                     KTGFTNLKLDTVAIRGFNEDELSALIEFAK"
     gene            complement(646..>1024)
                     /locus_tag="VFT30_03330"
     CDS             complement(646..>1024)
                     /locus_tag="VFT30_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU5091687.1"
                     /translation="ELDRVRSRSKRIGGIATFLGIARDRSRGRDVDGITFEHYEGMAQ
                     KKLREIRERALKDFDILELLIIHRYGEIAIGENIVLIIAGAEHRADAFRACKWAIDEL
                     KQITPIWKLEHTPEGEVWVEEHP"
BASE COUNT          229 a          318 c          234 g          243 t
ORIGIN      
        1 gtttcgcaaa ctcgatcaat gcgctcaatt catcctcatt gaacccgcga atcgcgacag
       61 tatcgagctt caaattggta aagcccgttt tcccgactga ttcaattcct tccaggactc
      121 gtgcaaattc atcccgcccg gtcagtcggc gaaatcgctc cggcctcagg gtatctaagc
      181 tgaccgtgat tcggttgagc cccgcatcgt agagctcctg tacgtattca gccaagagca
      241 cgccgttcgt cgtcaacgcc acttcggtaa tgcgccgatt ctgaaggagc aatcgtacca
      301 gccgcgcaag gtcccgccgc aacaaaggct cccccccggt caatctgact ttgtcgaccc
      361 ccagatcggt gaaatatccc gcgagcgtcg tcatttcctc gaaactgagg atatcctcgc
      421 gtggcagcca gacatactcc tcttccggca tgcagtactt gcagcggaga ttacaacgat
      481 cagtgacgga caaccgcaaa ttcttcaacg gccgactgaa cgtatcggtt atgccttcca
      541 caagatcact ttgagaagaa tcttctttca tctatctctc atcaggcaag atggtgaggc
      601 ggcaatgtct cgcgggaacg cagctggacg ccagctcacc atcctctagg gatgttcctc
      661 cacccagacc tccccttccg gcgtatgttc cagtttccag atcggtgtga tttgctttaa
      721 ctcatcgatc gcccatttac aggctcggaa tgcgtcggcg cggtgctcgg caccggcaat
      781 gatcagcaca atattttcac cgatcgcaat ttctccatac ctgtggatga tgagcagctc
      841 cagaatatcg aaatctttca aggcccgttc tcgaatttca cgtaacttct tctgcgccat
      901 cccttcgtaa tgctcgaacg taatgccatc gacatctcgc ccgcgagacc gatcgcgcgc
      961 gatgcccaaa aacgtggcaa tcccgccgat tcgtttggac cggcttcgaa cgcgatcgag
     1021 ttct
//
LOCUS       DASTWE010000309         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_751967, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000309 DASTWE010000000
VERSION     DASTWE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751967"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /gene="ispD"
                     /locus_tag="VFT30_03335"
     CDS             complement(<1..615)
                     /gene="ispD"
                     /locus_tag="VFT30_03335"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5091688.1"
                     /translation="MNNHTVALVPAAGRGLRMGGSVPKQFLALGGQPLILHSLRVFQA
                     SSVIEEVILAVPLNEMDYCLKEIVAKHRFTKVTKVVPGGHERQDSVRHALEAVHDDVD
                     VVLVHDAVRPFLTEHMVEEVVKAAWAKGAAIIALPMKDTVKQVGADHVIERTVDRQSL
                     WLAQTPQAFRRDWLLAAHRKAHAEGIRATDDAYLIEWAGHPVSVV"
     gene            complement(720..>1513)
                     /locus_tag="VFT30_03340"
     CDS             complement(720..>1513)
                     /locus_tag="VFT30_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5091689.1"
                     /translation="GNSGGALVNVRGELVGINTAIFSQSGGNMGIGFAVPSNMAQSIM
                     GQLVQTGKVVRGWLGVSIQELTPELASQFGITETKGVLVSDVMDDSPAKKAGFERADV
                     IVEYDGKPMDSPTHLRNAVAQTPVGKKVTIKFIRDKKAKTVDLTVVEQPKSMSQNGED
                     DGGESTTPTGVLSSLDVRDLTQELASRYGLKSSERGVVIVRVRPGSTAEELGVREGDV
                     VLEVNRQAVTSVKVFEKIAGKLPKDQAVLLLLKRQGRTIYLTLRP"
BASE COUNT          319 a          476 c          375 g          343 t
ORIGIN      
        1 gacgacagac accggatgtc cggcccactc gatgagatag gcatcatcgg tggcacgaat
       61 cccttctgcg tgggccttcc tgtgggctgc caacagccaa tcgcgacgga aggcctgcgg
      121 cgtttgagcc agccacaatg actgccgatc aaccgtgcgc tcgatgacat ggtcggctcc
      181 cacttgtttg acggtatcct tcatgggaag ggcgataatt gccgctccct tggcccatgc
      241 cgccttcaca acctcttcca ccatatgctc tgtgagaaac ggacgaaccg catcatgcac
      301 caacaccaca tcgacatcat catggactgc ctcaagcgca tgcctgacgg aatcttgccg
      361 ttcgtgtcct cccggtacca ctttagtcac cttggtaaag cgatgcttcg ccacgatttc
      421 tttgaggcaa tagtccatct cgttcagggg aaccgccaga atgacttcct cgatgacgga
      481 agaagcttga aacacacgga gcgaatggag gatgagaggc tgaccgccga gtgcaagaaa
      541 ttgtttgggc accgagccgc ccatgcgaag gcctcgtcct gcggcaggga caagagccac
      601 ggtgtggtta ttcacgactg aagtctagac tatccttact cattgcgggg agcgtactaa
      661 cgcagattgc ccgcccaact ttgtctccaa catccaaaat ctgactacct ggcaagtcct
      721 catgggcgaa gcgtcaggta aatcgtcctc ccctggcgtt tcaacaagag caagactgct
      781 tgatccttag gcagcttgcc tgcgatcttt tcaaaaacct tcaccgacgt cacagcttga
      841 cgattcactt cgaggacgac atcgccctcc cgaacaccca attcttcggc cgtgcttcca
      901 ggcctgaccc gaacgatcac caccccccgc tcactcgact tcagcccgta ccggctcgcc
      961 aactcttggg tcaagtctcg gacatcgagg ctggagagca cgccggtcgg cgtggtcgat
     1021 tcccctccat catcttcgcc gttttgcgac attgacttgg gctgctcgac gacggtgaga
     1081 tcgaccgttt tcgctttttt atcgcgaatg aacttgatcg tgactttttt cccaaccggt
     1141 gtttgagcca cggcattgcg caggtgtgtc ggtgaatcca tgggtttgcc gtcatactcg
     1201 acgatgacgt cggctcgttc gaacccggcc tttttcgccg ggctgtcgtc catgacatcg
     1261 ctgacgagca cgccctttgt ctccgtgata ccaaattggg aggctaattc cggcgtcaac
     1321 tcctgaatag ataccccaag ccaaccacga acgacttttc ccgtttgtac gagctgcccc
     1381 atgatggact gcgccatatt actcggcacg gcaaatccaa tccccatatt cccgccgctc
     1441 tgactgaata ttgcagtgtt gattcccacc agctcgcccc gcacattgac caaggctccg
     1501 ccggagttgc ccg
//
LOCUS       DASTWE010000310         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_719443, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000310 DASTWE010000000
VERSION     DASTWE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719443"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1037
                     /locus_tag="VFT30_03345"
     CDS             <1..>1037
                     /locus_tag="VFT30_03345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5091690.1"
                     /translation="AEIIKVELMPNGDISRNLPFIKNGRSGYYIQQNRGKKSLCIDAR
                     NPKGQEILKALIKKVDVVVENFSPGTIARLGFGYDVVKELNPRVVMCSVSALGQTGPL
                     ATIPGYDYIGQAYAGVTYMIGDPNGAPAVPMLGLGDVSTGVHAYAAIASALLYRERTG
                     QGQYLDITLLDSYFHCHELSVQLYSGTNGAVEPKRSGSHHYAISPAGLFKGKNQYLFI
                     LCLDHQWQVLCRAMGKPELIEDPRFANNAKRVEHQKEVIQVIEGWIQAQASDDEALRI
                     LHEGHVPAAPVLSIPEAINHPHLKARRTVRRIKDNKFGELDIPGMPLKFSQFPEELSL
                     QAAALGEHNEE"
BASE COUNT          255 a          290 c          283 g          209 t
ORIGIN      
        1 gctgagatca tcaaagtcga attgatgccg aatggcgaca tctcgcggaa tctgcccttc
       61 attaagaacg gtcgcagcgg ttactacatc cagcagaacc gcggcaagaa aagcctgtgt
      121 atcgacgcgc gtaatcccaa gggacaggaa atcctcaaag ccttaatcaa aaaagtggac
      181 gtcgtcgttg aaaacttttc gcccgggacc attgctcgct tgggattcgg ctatgacgtc
      241 gtcaaagagc tgaacccacg ggtggtgatg tgttcggtct cggcgctcgg gcagactgga
      301 ccgctcgcca ccataccagg gtacgactac atcgggcagg cgtacgccgg ggtcacctac
      361 atgattggtg accccaacgg ggcaccggca gttcccatgc tcggcctcgg cgacgtcagc
      421 accggagtac atgcctatgc ggcgatcgcg agtgccctcc tctaccgcga acgtaccggg
      481 caaggacagt atttagatat cacgctgcta gattcttatt ttcactgtca tgaactcagt
      541 gtccaattgt acagcggcac caacggagcg gtggaaccga agcggtcagg ctctcaccat
      601 tatgcgataa gtccagccgg gttattcaag ggtaagaatc aatacctctt cattctctgt
      661 ttggaccatc agtggcaggt cttgtgccgg gcaatgggca agcccgaact gatcgaggac
      721 ccacgcttcg ccaataacgc gaaacgcgtc gagcatcaga aagaagtgat ccaggtcatt
      781 gagggctgga ttcaagccca agcgagcgac gacgaagcgc tgcggatact tcacgaaggg
      841 cacgtaccag ccgccccggt gttatcgatt cccgaagcga tcaaccaccc gcatctcaaa
      901 gcccggcgca ctgtccgacg gatcaaggat aataagtttg gcgagcttga tattccgggc
      961 atgccactga aattttccca gttccctgaa gaattgtcgt tgcaagccgc agctctcggc
     1021 gagcacaatg aagagat
//
LOCUS       DASTWE010000311         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1010485, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000311 DASTWE010000000
VERSION     DASTWE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010485"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFT30_03350"
     CDS             complement(<1..367)
                     /locus_tag="VFT30_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091691.1"
                     /translation="MDRAHELDDLMEKMVATYVVRNRAAGVLKAMLDETGVGFSPVID
                     HVTIRTFDIDRGAEPFVKLGYAYDETLQYDDWYAKVYRKVGYPALFVDQAYPDARGKT
                     SIIPGWVNKFGDKVFHHVAV"
     gene            complement(381..>1903)
                     /locus_tag="VFT30_03355"
     CDS             complement(381..>1903)
                     /locus_tag="VFT30_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5091692.1"
                     /translation="IHTALRDLGIQAVNSGGSTGNGWWSGRNDGSLLPSINPATGEQI
                     AGVYPCSFDDYQRIVKESVEAFRTWRMVPAPKRGEMVRLIGQALREKKDQLGTLVSLE
                     VGKIKAEGDGEVQEMIDMADFAVGQSRMLYGVTMQSERPAHRMSEQWHPLGPVGVITA
                     FNFPVAVWAWNAFVATIAGDTVIWKPSPKAPLCAVAVQQICNRVMQQQGYSGIFSLFI
                     ADQPALAEQMVQDERLPLISFTGSVPVGRRVASLVGQRLGRTLLELSGNNAVIVDETA
                     DINMAVRAILFGAVGTAGQRCTTTRRLIVHESRYEELVGRLVKAYAQVQIGNPLEKDV
                     LMGPLIDHVAVEGYRAALDEIKKEGGEILCGGHVLNRPGYFVEPTIVRAQNQWPIVQR
                     ETFAPILYVMTFQTIDEAVQMQNGVPQGLSSAMFSNDVRHGERFLSAAGSDCGIANIN
                     IGTSGAEIGGAFGGEKDTGGGREAGSDSWKAYMRRQTNTVNWGTELPLAQGIKFGS"
BASE COUNT          399 a          578 c          504 g          422 t
ORIGIN      
        1 gaacagccac atggtgaaag accttgtcac cgaatttatt gacccagccg ggaatgatgc
       61 tggtcttacc tcgcgcgtcc ggataggctt gatctacgaa tagggcgggg taaccgacct
      121 tgcggtagac cttcgcgtac caatcgtcat attgcagggt ttcatcatag gcgtagccca
      181 gcttcacgaa gggttcagct ccacggtcga tatcgaacgt ccggatggtg acatggtcaa
      241 tgaccggcga aaagcctacg ccggtctcgt ccagcattgc cttcagaacc cctgccgccc
      301 gattacgaac gacataggtc gccaccatct tttccatgag atcatcgagt tcatgagccc
      361 gatccatggt cgcacctcgt tcaagatcca aacttaatgc cttgggccag aggtaattcc
      421 gtaccccagt tgaccgtgtt ggtctggcgg cgcatatagg ctttccagga gtccgatcca
      481 gcttcgcgtc ctcctccggt gtccttctcc ccaccgaacg ctccgccgat ctcggctccg
      541 gaagttccga tattgatgtt ggcaattcca caatcgcttc cggccgccga cagaaaccgc
      601 tcgccgtggc gcacatcgtt cgaaaacatc gccgatgaga ggccttgcgg gacgccgttt
      661 tgcatctgaa cggcctcgtc aattgtttgg aacgtcatga cgtatagaat aggcgcgaaa
      721 gtctcacgct gcacaatggg ccattgattt tgcgctcgga cgatcgtcgg ttccacaaaa
      781 tacccgggcc gattcaatac atgtccgccg cagaggattt cgccgccttc tttcttgatt
      841 tcatcgagtg ctgcacgata gccctccacg gccacatggt cgatgagcgg ccccatgagc
      901 acgtcttttt ccagcggatt gccgatctgc acttgggcat aggccttcac gagcctgccg
      961 actaattcct cgtagcgtga ttcgtgaacg atcagacgtc tcgtcgtcgt gcatcgctgc
     1021 ccggcagttc caacggctcc gaataagatg gcgcgcaccg ccatgttgat atccgctgtc
     1081 tcgtcgacga tcacggcatt gttgccgctg agttcgagga gggttcggcc caatcgctgt
     1141 ccgactaacg aagcgaccct tcgaccgacc ggtaccgatc cggtaaacga gatgaggggc
     1201 aaacgctcat cctgtaccat ctgctcggca agagccggtt ggtcggcgat gaacagcgaa
     1261 aaaattcccg aataaccctg ttgctgcatg acacgattac agatctgttg caccgcgacg
     1321 gcgcagagcg gcgctttcgg tgacggtttc cagataaccg tatcgccagc gatggtggcc
     1381 acaaacgcat tccaggccca cacggcaaca ggaaaattga acgcggtgat gaccccgacc
     1441 ggtcctaacg gatgccactg ctcgctcatc ctgtgggccg gccgctcgga ttgcatcgtg
     1501 acgccgtaga gcatccggga ttgacctact gcaaaatccg ccatatcgat catctcttgc
     1561 acttcaccat cgccctccgc cttgatcttg ccgacttcca gcgagacgag cgtgcctaat
     1621 tggtctttct tctcccgtaa ggcctggccg atgagccgga ccatctcgcc acgcttcggg
     1681 gccggcacca tcctccaggt gcggaaggct tcaaccgatt ctttcacgat ccgttgataa
     1741 tcgtcgaatg aacagggata gacgccggcg atttgctcac cggttgcagg attgatcgag
     1801 gggaggaggg atccgtcatt acgaccggac caccagccat tgccggtact cccaccggaa
     1861 ttcacggctt ggataccaag gtctcttagc gcagtatgta tgg
//
LOCUS       DASTWE010000312         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1042731, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000312 DASTWE010000000
VERSION     DASTWE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042731"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..456
                     /locus_tag="VFT30_03360"
     CDS             133..456
                     /locus_tag="VFT30_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091693.1"
                     /translation="MGFLQRLKDDLRTGVATLRLGTVHAAGRALEETELLRMRLELRK
                     LDQQLSDLYKDIGERAVDMKERGETAERVVYDAEIVRLVKEVEVLKESRKKLEADMEE
                     IRNEQ"
     gene            453..>1076
                     /locus_tag="VFT30_03365"
     CDS             453..>1076
                     /locus_tag="VFT30_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEU5091694.1"
                     /translation="MTEPHRHTRRLAGIDIGTLTCRLLITDLAPGQPLQEVLSDRRIL
                     RLGEGVDQTKRLSPAAMDRVIHCLQEWRNIINSYQVEASSVVATSAVRDASNRDEFLV
                     HIKREAGFDVEVISGEEEARRTLLGIRSGLPGGVTDILALDIGGGSTEFILDRQGQKP
                     IIRSIDIGVVRLCERLLRHDPPTDGEVSSAREWVTRETEAAVSDMGVY"
BASE COUNT          254 a          279 c          331 g          212 t
ORIGIN      
        1 ctaggcagga ggccttttcg ctgatgaaat cggatccagg gttgaaagag cctgcgcttc
       61 aagggttgcg tgacgcgatg ttgaggaaat gggagaaaaa actcgacctt gggtcgataa
      121 gctaggacat cgatgggatt cctccaacgc ctaaaagatg acttacgaac cggggtcgcc
      181 acccttcgct tagggaccgt ccatgctgcc ggccgtgcat tggaagagac ggaattgctc
      241 cgcatgaggc tggagctccg caagctcgat cagcaactct ccgatctgta caaagacatc
      301 ggcgagcgcg ccgtcgacat gaaggagcgt ggcgaaacgg ccgagcgtgt cgtgtatgac
      361 gccgagatcg tgcgtctcgt caaggaggtt gaggtgctca aggaatcgcg gaagaaactg
      421 gaagccgaca tggaagaaat tcggaacgag caatgaccga gccacaccgg cacacgcgac
      481 gattagccgg catcgatatc ggcaccctca cctgtcgctt gttaatcacg gatctagccc
      541 ctggacaacc cctccaagaa gtcctatctg accggcgcat tctccgcctc ggcgaaggag
      601 tcgatcagac caaacgcctg agtccagccg caatggatcg ggtcatccac tgtctacaag
      661 aatggcggaa tatcatcaac agctaccagg tcgaggcatc ctcggtcgtg gcaacgagtg
      721 ccgtccgcga tgcgtcgaat cgagatgagt tccttgtaca tatcaaacgg gaagccgggt
      781 tcgatgtgga agtgatttcg ggtgaggaag aagcgcggcg gaccttgctc ggcattcgat
      841 ccggcctacc gggtggagtg acggacatcc ttgcattaga tatcggtggc ggcagtaccg
      901 aatttatttt ggatcggcaa ggacagaagc cgattatccg ctcaatcgat atcggagtcg
      961 tccgtctctg cgaacgcctt ttgcgtcatg atccgccgac tgatggggaa gtgagctcgg
     1021 cacgcgagtg ggtgacacga gagacggagg cggctgtttc cgacatgggg gtttat
//
LOCUS       DASTWE010000313         1838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_170203, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000313 DASTWE010000000
VERSION     DASTWE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170203"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VFT30_03370"
     CDS             complement(<1..1017)
                     /locus_tag="VFT30_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEU5091695.1"
                     /translation="MNTDDYINLFDDLDDRDQTENFINKNKRPQRFPERHFKYVREAN
                     AGFLKGQDDSSRSFEFTYSAARFESWWLLESLGDFYEHKWITDVLQRVKGGKEASVYL
                     CRSGPAIQAPLVAAKVYRPRSLRNLKNDGQYRVGRTDLDADGNAIVRDGDVHAMQKRT
                     AYGEELRHQSWIAYEFQTLETLHAAGADVPRPYAMEKNAILMDYIGDIDSAAPTLNSV
                     SLESDEVKPLFERVIYDIELLLSNQRIHGDLSAYNILYWEGDITLIDFPQVVQPESNP
                     AAWAIFLRDITRVCQYFASQGLRRDPRRLAADLWTSHGHRVVQEVHPRDLDPEKKEDR
                     RLWEK"
     gene            complement(1319..1789)
                     /locus_tag="VFT30_03375"
     CDS             complement(1319..1789)
                     /locus_tag="VFT30_03375"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEU5091696.1"
                     /translation="MAEFHLAQINIARMLAPIDDPLMSDFVAQLPPINALAEDSPGFI
                     WRLQSEGGDATSIKVYEDDMIIINLTVWESLEALRQYVYKSAHSGVMRDRRRWFEKFD
                     GPYYALWWIPAGHRPDPQEGKERLEHLREHGDSPHAFSFKHVFDDRETEIGSNG"
BASE COUNT          423 a          518 c          493 g          404 t
ORIGIN      
        1 tttttcccac aaacgacgat cctccttttt ctccggatcg agatcgcggg gatggacctc
       61 ctggaccacc cggtgcccgt gcgaggtcca caggtcggcg gcaagccttc ggggatcgcg
      121 ccggagcccc tgggaggcaa agtactggca gacgcgcgtg atgtcgcgca ggaagatcgc
      181 ccaggcggcg gggttggatt ccggctgaac aacctgggga aaatcgatca acgtgatgtc
      241 cccttcccag tagaggatgt tgtaggcgga cagatcgcca tggatccgct ggttggacag
      301 gagcagctca atatcataaa tgacgcgctc aaagagcggc ttgacctcat ccgattccaa
      361 agacacagag ttgagcgtcg gcgcggcact gtcgatatcc ccgatgtagt ccatcaggat
      421 ggcgttcttt tccatcgcat agggtctggg tacgtccgcg cccgcggcgt ggagcgtctc
      481 cagagtctgg aattcgtacg cgatccagga ttggtggcgc agctcctcgc cataggcagt
      541 tcgtttttgc atggcatgaa catcaccgtc cctgacaatc gcattgccgt cggcatcgag
      601 gtctgtccgg ccgacgcgat actgaccatc gttcttgaga ttgcgcaggg agcgcggacg
      661 gtagactttg gcagccacca gcggcgcctg gatggcagga ccggaacgac agagatacac
      721 cgaggcctct ttaccaccct tgacccgctg cagcacatcg gtaatccatt tgtgctcata
      781 gaaatccccg agcgattcta acaaccacca tgattcaaat cgagccgccg agtaggtaaa
      841 ttcgaaactg cgactggagt catcctggcc tttgaggaat cccgcgttgg cttcacgcac
      901 atacttaaaa tgccgctccg ggaagcgctg aggtcgcttg ttcttattga taaagttctc
      961 ggtttgatcc cgatcatcca ggtcatcaaa cagatttatg taatcatccg tattcatact
     1021 ctcgttcatc aaagacctga cacgtctgga agcctgtcat tcttcacgca cctgcggaga
     1081 cctgtcaggt ctgggattaa aagaaaaacc gcagggttgc gaagcacgcc tgcggcaagt
     1141 agcaaatgag aacgacgaaa gtcgttacta cttcttaccc tgaggaggcg cgctcaaaac
     1201 acggttgctg gttgacaaga tagatttcat tttttcgtgc ctcctttcaa gagagaatta
     1261 gatagtgaga gtgtatcacc cggaacggag agtgtcaaga aaaacaaatc gcccagaatc
     1321 agccattcga accgatctcc gtttcacggt catcgaaaac atgcttgaac gaaaacgcat
     1381 gcggcgagtc gccgtgctcc cgcaggtgct cgagtctttc tttcccctcc tgcggatccg
     1441 gtctgtgccc ggcaggaatc caccacaggg cgtagtacgg accgtcaaac ttttcgaacc
     1501 agcgcctgcg atcccgcatt acgccgctgt gcgcactctt atacacatat tgccgcagcg
     1561 cctccaggct ttcccatacc gtgagattga taatgatcat gtcatcctca taaactttga
     1621 tgctggtcgc atcgccgcct tccgattgca gccgccagat gaagcccggg ctgtcctctg
     1681 ccagcgcgtt gatcggcggc agctgcgcga cgaaatccga catgagagga tcatcaatgg
     1741 gggcgagcat gcgggcgatg ttgatctgtg cgagatgaaa ttcagccatt tctaaccgca
     1801 agaatttact gtcctaatcc cgaaaaatcc agcgcacg
//
LOCUS       DASTWE010000314         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1010551, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000314 DASTWE010000000
VERSION     DASTWE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010551"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFT30_03380"
     CDS             <1..759
                     /locus_tag="VFT30_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5091697.1"
                     /translation="ALRLPTMASLVTGMPVEKHGITWNFFEFSRGYPRYPSLFDYLDL
                     SGGRDSAIFYMDESLYQLAKPEPYTDYQLCGALRPECGSQKLVAYIQQYLQKATSGHG
                     YGHAIPSLPHLLVVHLPEAGRAGVTHGWNSKEYREALRTVDGAMKSVLDLFKEYSLLN
                     RTAVVVTALSEKGTDPGAEASTTDSPVVPWIVSGAGIKHGQLIRQPVSIIDTGATVMK
                     ILGLETHTEWESKAVEEIFQTAAAIPAVRPLKKR"
     gene            802..1236
                     /locus_tag="VFT30_03385"
     CDS             802..1236
                     /locus_tag="VFT30_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091698.1"
                     /translation="MVFVCALISLLIIPGSEEGFAGDPPAAHLREYGITIDATKLVPT
                     SWWQVPGVTPSIWNSDPESLDAPKTSELRELRLKPGKYKFISFTFDFPFTVTLNGTLD
                     FSKSLDQCVEGRGTQTLVVVCKRMYPHGGQRDAYYEQKPNDG"
     gene            1229..>2291
                     /locus_tag="VFT30_03390"
     CDS             1229..>2291
                     /locus_tag="VFT30_03390"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5091699.1"
                     /translation="MADVLEDLLEALEDVDDATREEAARMLADMGDPTTLDALISACN
                     DDFWSVRAYAGCGVAKIGGQKAVEALIGLFNDSIMEVRDQAVEATAKMGPIVLDRLVA
                     AVKDERWRIREHAAKAAGKIKDSRAVDALIDACRDRDGAVKSAAAEALGRIADPKAIP
                     VLIKLFRDSSKIVRETAGTALVYIGHPSVDPLIQSLTDKDFVVRCHAARALGGMTTDY
                     QIGRSWVRDAKVVDALIAALKDPDRAVREDATIALGMIGDPRAIDALVEAMKDGAVKR
                     HAIASLGMIGDSRALPAVLDALKGKGIKQEGSPTPGCIVSEDAFIKEAAATALGQFRN
                     PRVIPDLIMLLKDGVLREKA"
BASE COUNT          520 a          642 c          656 g          473 t
ORIGIN      
        1 gccctacgat tgcccacaat ggcatcttta gtgaccggca tgccggtgga gaaacatggg
       61 attacctgga atttcttcga attcagccga ggctatcctc gctatcccag cctattcgac
      121 tacctggatt taagcggagg ccgcgacagt gccatctttt atatggatga gtccctgtat
      181 cagctcgcca aaccggaacc ctataccgac tatcaactct gcggggcatt gcggcccgag
      241 tgcggttctc agaaactcgt cgcctacatc cagcagtatc tccagaaggc gaccagcgga
      301 cacggatatg ggcacgcgat tccctccttg cctcatttgc tggtggtaca ccttccggaa
      361 gcagggaggg caggagtgac ccatgggtgg aactctaaag agtatcgcga ggcgttacga
      421 acggtcgatg gcgcgatgaa gtctgtcctg gacctcttca aggagtactc tctcttaaac
      481 cgaaccgccg tcgtcgtcac cgctctcagc gaaaaaggga cagaccccgg cgccgaagcc
      541 tccacgactg attcgccggt cgtcccctgg atcgtctcgg gagccggaat aaaacatggc
      601 caactcattc gtcagccggt gtccatcatt gataccgggg ccacggtgat gaaaatcctg
      661 gggctagaga ctcacacgga atgggagagt aaagctgtag aagaaatctt ccaaaccgcc
      721 gcggccattc cagccgtacg acctctcaag aaacgctaat catctatgta ctatgctgcc
      781 ctcatacgac gctggttatt catggtattc gtctgtgcgc tgattagcct gttgattata
      841 cctggttccg aagaaggatt cgcgggagac ccgccggccg ctcatcttag agaatacggg
      901 attaccatcg atgcgacgaa gctggtcccg acctcctggt ggcaagtacc aggagtgaca
      961 ccttcgatct ggaattccga ccccgaatca cttgatgcgc caaagacatc cgagcttcgg
     1021 gaactacggt tgaagcctgg taaatacaaa ttcatcagtt ttactttcga ttttcctttc
     1081 acagtaactc tcaacggcac tctcgatttt tcgaaatcat tagatcaatg tgtcgaaggc
     1141 cgcgggacac agactttggt ggtggtgtgc aaacgcatgt acccacacgg cgggcaacgc
     1201 gacgcctatt acgagcaaaa accaaacgat ggctgacgta ctggaagatc ttttagaagc
     1261 gcttgaagat gtggacgatg ccacccgtga ggaggcggca aggatgcttg ccgacatggg
     1321 tgatccgacc accttggacg ctctgatcag cgcctgcaac gacgatttct ggtccgtgcg
     1381 ggcctatgcc ggctgcggcg tcgcgaaaat cggcgggcag aaagcagtcg aagctctgat
     1441 tggccttttc aacgattcca tcatggaagt tcgcgatcag gcggtcgagg ccacggcgaa
     1501 aatgggtccg atcgtgctcg atcgcctggt ggctgccgtg aaagacgagc gttggcgcat
     1561 acgcgaacac gcggccaagg ccgccggcaa gatcaaggac tcaagagccg tcgacgcgct
     1621 gatcgacgcc tgtcgcgaca gggacggggc ggtcaagagc gctgcggcgg aagccttggg
     1681 cagaatcgcc gatccgaaag ccattccggt cctgatcaag ctgtttcgtg attcttctaa
     1741 aatcgtgcgc gagacggccg gaacggcgct ggtctacatc gggcatccct cggtcgatcc
     1801 cttgattcaa agtctcacgg acaaagactt tgttgttcga tgtcacgcgg cccgtgcgtt
     1861 gggcgggatg accaccgact atcaaatcgg caggtcctgg gttcgggacg cgaaggtcgt
     1921 ggacgccttg atcgccgcct tgaaggaccc tgaccgagcc gttcgcgaag acgcgaccat
     1981 tgcgttaggc atgatcggcg acccccgagc aatcgacgcg ctggtagaag ccatgaaaga
     2041 cggagcggtg aagcgccatg ccattgcctc actcggcatg atcggtgatt cgcgcgccct
     2101 cccagccgta ttggacgcct tgaagggcaa aggcatcaag caggagggct cgccgacacc
     2161 agggtgcatc gtcagtgaag atgcgttcat caaggaagcc gccgccactg ctctcggtca
     2221 gtttcgcaat ccccgggtga ttccggattt gatcatgtta ctgaaagacg gggtcttgcg
     2281 agaaaaagcc g
//
LOCUS       DASTWE010000315         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_235044, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000315 DASTWE010000000
VERSION     DASTWE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235044"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VFT30_03395"
     CDS             <1..1074
                     /locus_tag="VFT30_03395"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU5091700.1"
                     /translation="PIAEIALDTVSVVKDGRTIQRFRELEIEQRQGDEASLRSLEQRM
                     REAGASDHDGRPKFFRALSLPAPTPASQPEPDKPVSEHLKWALARHVGWLLAHDPGTR
                     LGTESEGLHQLRVATRRLRAVLRTAQPILLPLWATSLQQELDWLSDLLGPARDLDVQI
                     AYFTDESTALDARDRKLLAQFIAHLRIQREAVQQMVLGELMSARYLELIRRLQQAVHD
                     PSMVESHMRVRNLAKREFKKLRKVIHRLGPSPSDTQIHKVRIKTKRARYAAELAHSSV
                     GKPATRFIKSARTVQDLLGIHQDAIQAELHIRQFLKYSTSVRAGFLAGRMAERQRHRR
                     QRVRKDMKPLLKRLLKRGKKVWV"
     gene            complement(1082..>1592)
                     /locus_tag="VFT30_03400"
     CDS             complement(1082..>1592)
                     /locus_tag="VFT30_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091701.1"
                     /translation="MEKANSPEDVKKVLQQASLATRVGADPEKEPCGASAVLRTKRYR
                     LEAFGRQEAAESKKRKTDVRMPEEFIQKVMDMPNMEMDVQLCGDDEYFAEGALIELQQ
                     GSKRIKPIDIGKAERGRKNEGNGPAFRSRFTAIFAYEQFDPTASSVFVVNLQDGKEIR
                     IPADFSKVK"
BASE COUNT          335 a          487 c          423 g          347 t
ORIGIN      
        1 cccatcgccg agatcgccct cgatacagtg tcggtagtca aagacggccg tacaattcag
       61 cgattccgcg agctcgaaat cgaacaacgg cagggcgatg aagcctcgct gcgttccctc
      121 gaacaacgga tgcgtgaagc cggtgcttcc gatcacgacg ggcgacccaa gttctttcgt
      181 gctctttcgc tcccagcccc taccccagca tcgcagcctg agccggacaa gccggtgagc
      241 gagcatctga agtgggcact cgcccggcac gtcgggtggc tcctggccca tgatcccggc
      301 acgaggcttg gaaccgagag tgagggcctg catcaactgc gtgttgcaac gagacgcctg
      361 cgggccgtgc tgcgcaccgc acagccgatt ctccttccct tgtgggcaac atcgctacag
      421 caggagctcg attggctcag tgacttgctg ggaccggccc gcgatcttga cgtccagatc
      481 gcctatttta ctgatgaatc taccgcattg gatgcgcgag atcgcaagct gttggcccaa
      541 ttcatcgccc atcttcggat tcaacgagaa gccgttcagc agatggtcct cggcgagctc
      601 atgagcgcac gatacctcga gctcatccgg aggcttcaac aggccgtgca tgatccgtca
      661 atggtggagt ctcacatgcg cgtacgcaac ctcgccaagc gggagttcaa gaaactacgc
      721 aaggtgatcc accggctcgg tccatctcca tcggataccc agatccacaa agtccgaatc
      781 aagaccaaac gcgcccgcta cgcggcggaa cttgctcata gctcagtggg caaacccgcc
      841 acccggttta tcaagtccgc gcgtacggtc caagatctat taggtatcca tcaagatgcg
      901 atccaagcgg aacttcacat tcgtcagttt ctgaagtact cgacgagtgt gcgcgccgga
      961 tttctcgccg gacgcatggc tgagcggcag cgccatcggc gccaacgggt ccgcaaggac
     1021 atgaagccgc tgctcaagag gttgctaaaa cgaggaaaaa aagtttgggt gtgaccggag
     1081 cttatttcac tttggagaaa tcggcaggaa ttctaatctc tttaccgtct tgtagattga
     1141 cgacgaacac cgacgatgca gtggggtcga actgttcata cgcgaaaatc gccgtgaacc
     1201 gtgacctgaa ggctggacca ttgccttcgt tctttcgccc ccgttcggcc ttgccgatgt
     1261 cgataggctt gatccgcttc gatccttgct gcaattctat caatgcgccc tccgcgaaat
     1321 actcgtcatc gccgcataac tgcacatcca tttccatgtt gggcatgtcc atcactttct
     1381 ggataaactc ctcgggcatg cgcacatccg tcttccgttt cttcgattct gccgcttctt
     1441 ggcggccaaa ggcttccaat cgataccgct tggtacgaag aacagcgctc gcgccgcagg
     1501 gttctttctc cgggtcggca cctacgcgag ttgctaagga cgcttgctga aggactttct
     1561 tcacgtcttc cggcgaattg gccttttcca tc
//
LOCUS       DASTWE010000316         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_396371, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000316 DASTWE010000000
VERSION     DASTWE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396371"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="VFT30_03405"
     CDS             complement(<1..>1147)
                     /locus_tag="VFT30_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEU5091702.1"
                     /translation="DPFIGRLSYCRVLSGTIHADATVLNASKHVREKLGHFYTVLGKR
                     HVAVDSAKGGEIIAIGKLKDTHTGDTLCQENAPICYPVLSLPKPILSFAIESKSKTDI
                     DKVSLGLHKLIEEDPTLEFARNAETKEMVLSGMGQFHIDLALEKLHRKFGADVMLHTP
                     KVPYRETLRTKSQAQGKYKKQTGGHGQYGDCWLEVAPLPRGNGYVFENRVVGGAIPRN
                     FIPAVEKGVIEAMQEGPLSGFPVVDVQVAVYDGSYHVVDSSEMSFKIAGSMAFKKAIE
                     TAHPVLLEPVMTVEIDTPTDAVGAVMGDLSARRGRIVAVNAQDHAEQITALVPLAELL
                     RYATALNAMTGGRGSYIMDFAQYDEVPRELAARVIERHKAEGQAATAH"
BASE COUNT          238 a          338 c          288 g          283 t
ORIGIN      
        1 gtgggccgta gcggcttgtc cctctgcctt atgtcgctcg atgacccttg cagccaactc
       61 tcgtggcact tcatcatact gcgcgaagtc catgatatag ctgccccttc ccccagtcat
      121 ggcattgagg gccgtcgcat atcgaagtag ttcagcaagc ggaaccaacg ctgtaatctg
      181 ttccgcatgg tcttgtgcgt tgacggccac gattcgaccc cgtcgggcgc tcaagtctcc
      241 catgacagct ccgaccgcgt cggtcggtgt gtcgatttcg accgtcataa caggctcaag
      301 taacactgga tgcgcggtct ctatcgcttt tttgaatgcc atcgatcccg cgatcttgaa
      361 cgacatctcg gatgaatcca ccacatgata ggatccgtca tagacggcca cctggacatc
      421 aacgaccgga aacccgctca acggtccctc ctgcatggcc tcgatgaccc ctttttccac
      481 ggcgggaatg aaatttcgag gaatcgctcc tccgaccacg cgattctcaa acacataccc
      541 attgccacgc gggagcggcg ccacttcaag ccagcaatcg ccgtattgcc cgtgaccacc
      601 ggtttgtttc ttatacttgc cctgtgcctg tgatttcgtc ctgagcgttt cccggtacgg
      661 gaccttcggc gtatggagca tgacatcggc tccgaacttt cgatggagtt tttcgagggc
      721 caaatcaata tgaaattgcc ccatgccgct gagcaccatc tccttggttt cggcattgcg
      781 tgcgaattcc aacgtgggat cttcctcgat gagtttgtga agaccaagac ttaccttgtc
      841 tatgtccgtc ttggacttgc tttcaattgc aaacgacaag atcggcttgg ggaggctcag
      901 cacgggataa cagatgggag cattctcttg acacagtgta tcacccgtat gcgtatcttt
      961 gagttttccg atcgcgatga tctctccccc ttttgcggag tcgacggcca catggcgttt
     1021 gcccaagacc gtgtaaaaat ggccgagctt ctcccgaaca tgctttgaag cattcagcac
     1081 cgtcgcatcg gcatggattg ttccggacaa gacacggcaa taggacagcc gaccgatgaa
     1141 tgggtcg
//
LOCUS       DASTWE010000317         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1333592, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000317 DASTWE010000000
VERSION     DASTWE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333592"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..977
                     /locus_tag="VFT30_03410"
     CDS             111..977
                     /locus_tag="VFT30_03410"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5091703.1"
                     /translation="MASSAPLSLDSIRSTLATTSLGHALYLHQDLPSTNREALSLAQN
                     GARHGTVVIAESQSGGYGRHGRTWFSPPGLNIYCSVIVRGTGENLSLSEWLSWVPLAS
                     ALAVAEATQQMTAVSLSLKWPNDLLLHERKVGGILCESSLAITNDPVVVIGIGLNVNV
                     PLVSFPEELRSIAASLMEASRRPIDRNRFIAQLLVELEHCLGELRAHGVVRLRQAYTA
                     RCATLGRRVRVLFTNEKPIVGIAETVSADGALQVRPLSPHPRSQLIPLVDVRAADVIH
                     LRVAKEHGGRVE"
     gene            983..>1139
                     /locus_tag="VFT30_03415"
     CDS             983..>1139
                     /locus_tag="VFT30_03415"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU5091704.1"
                     /translation="MLLTIDIGNTNVVAGIFDGSTLLTHWRLATDPKTTADEYGVLCL
                     SLMARDGR"
BASE COUNT          230 a          338 c          311 g          260 t
ORIGIN      
        1 ccggtgccga tcgcatctcc atcggagcgc tcacccattc tgccccggct gcgacggtca
       61 gccttgtcat gacgttggct cggccttcac gacgtcggcg tgcatagtcg atggcgtctt
      121 ccgcaccact cagcctcgat agtattcgca gtacccttgc gaccacgtca cttggtcacg
      181 ccttgtatct ccaccaagat ctcccctcga ccaacaggga agcattgtcg cttgctcaga
      241 atggcgcacg gcacggtacc gtcgtcattg ctgaaagcca atcaggtggc tacggccgac
      301 atgggcgcac ctggttctct ccgccaggtc tgaacatcta ttgctccgtc attgtacgtg
      361 ggacgggaga aaacctttcc ctttcggaat ggctatcctg ggtaccactt gccagtgcac
      421 ttgccgttgc cgaagccact caacaaatga cggccgtgtc cctttcgctg aaatggccga
      481 acgacctgct tctccatgaa cgtaaagtcg gtgggatcct ttgcgagagc agcttggcca
      541 taaccaatga tccggtcgtg gtcattggaa tcggacttaa cgtgaatgtc cccctggtgt
      601 cttttccgga agaattgcgg tcgattgcgg cctcattgat ggaagcttcg cgacgaccga
      661 tcgaccgaaa tcgattcatt gcacaactgc ttgtggagct tgaacattgt cttggcgagc
      721 ttcgagctca tggagtggtc cggctacgac aggcctatac ggctcgctgt gccacgctgg
      781 gacgccgagt tcgcgtcctg ttcacgaacg agaaaccgat cgtcggcatc gcagaaacgg
      841 tctccgccga cggtgcgctt caggtgaggc cgttgtcacc tcacccgcgc tcacaactga
      901 tacctctcgt cgatgtccgt gcagccgatg tcattcatct gagagtagcg aaagaacacg
      961 ggggccgggt agaatgaatc cgatgctatt gacgatcgac atcggaaata ccaacgttgt
     1021 cgcaggaatc tttgacggat cgacactatt gacccattgg cggttggcga ccgatcccaa
     1081 gacaactgcc gatgagtacg gtgttctttg tctcagcctc atggcccgag acgggcgcg
//
LOCUS       DASTWE010000318         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1527561, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000318 DASTWE010000000
VERSION     DASTWE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527561"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..>1520)
                     /locus_tag="VFT30_03420"
     CDS             complement(169..>1520)
                     /locus_tag="VFT30_03420"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5091705.1"
                     /translation="IEGEDCRISSIRDVKDIGFGVRVLYHGAWGFAASSILSLEEVPR
                     VADLAVEIARGSASVALEKVRLASEPVHRDRVVTPYRIDPFNVPLEKKTDLLLNTMEV
                     LQRQSGIARSSASLWARRDRKLFVSTEGSHLEFDLLAGQGDCAATALHEGRFASRSFN
                     TPYLRKGYELIEEADFLREASRVASQAVEKVKAPAVDTGQYDLVLDPEHLSLTIHESC
                     GHPSELDRALGYEANYAGTSFLTTEKRGNFRYGSRQVNLVADNTEPETLGATGYDDDG
                     VACQKWDIIRHGIFVGYCTNREVAPKIGESRSYGSNRADGWGNIPIVRIANIGLEPGE
                     ATRDQLIADVKRGIYIEGHGSYSIDQRRYNFQFGGDAFWLIENGRRTHMLRDVIYHGI
                     TPEFWNSCDGVADRSFRRRYGFITCGKGQPGQSGWMTHAASPARFRQVQVIRGEESS"
BASE COUNT          339 a          474 c          373 g          334 t
ORIGIN      
        1 catcgacatt ttgaatgact tgattattgg cgaacctggt cgttccggca tggtgatcgc
       61 gaagcctgac gatcgtatga tcagcggacg agcgggtcat gaccagatcg ctcagaaaac
      121 gaaactcgtc agagccagtc atcttcggcc aagtgccgct cagtatggtc acgagctttc
      181 ttctcctcgg atcacctgga cctggcgaaa tctcgcggga gaggcggcat gggtcatcca
      241 gccggactgg cccggctgcc ccttcccaca cgtaatgaat ccgtaccgtc gacgaaaaga
      301 tcggtcagcc accccgtcgc agctgttcca gaattccggc gtaatcccat ggtagatcac
      361 atcccgtaac atatgggttc tcctgccgtt ttcgatcagc caaaacgcat caccgccgaa
      421 ctggaaattg tagcggcgct gatcaatgct gtaggaccca tggccttcga tatagatgcc
      481 gcgtttcacg tcggcaatga gctgatcacg cgtcgcctcg ccaggctcaa gtccgatatt
      541 ggcaatccgc acgatcggaa tatttcccca tccatccgcc cgattggagc catatgaacg
      601 agattcgccg atcttcggcg cgacttcacg gttcgtacaa tatccgacga agatcccgtg
      661 acgaatgatg tcccacttct gacatgccac tccatcatca tcataaccgg tcgcgcccag
      721 agtttccggt tctgtgttat cggcaactaa attcacctgc cgcgagccat agcgaaagtt
      781 gccacgcttc tccgttgtca agaaacttgt accggcataa ttggcctcgt agccaagcgc
      841 tcgatccagc tcgctcggat ggccacagga ttcatgaatg gtcaatgata gatgttcagg
      901 atcgagcacc agatcatact ggccggtgtc aaccgccggc gcctttacct tttcgaccgc
      961 ttggctggcc acacgtgacg cttcccgtag gaaatctgct tcctcgatca gctcatagcc
     1021 tttcctgaga tatggcgtgt tgaagctgcg cgacgcaaat cggccttcat gcaatgccgt
     1081 cgccgcacag tcgccctgtc cggccaggag atcaaactcc aagtgcgacc cctcggtcga
     1141 gacaaacaac tttctgtccc gacgcgccca aagacttgcg ctgctcctcg cgatgccgga
     1201 ttgccgttgc aacacttcca tagtgttgag caacaagtcg gtcttttttt ccaacggcac
     1261 gttgaaggga tcgatgcgat acggtgtgac gactcggtca cggtgaaccg gctccgacgc
     1321 caatcgcacc ttctcaagcg ccaccgatgc agaccctctg gcgatctcaa cagccaggtc
     1381 cgccacccgc ggtacctctt ccaacgacaa gatcgaactc gcggcaaacc cccaggcccc
     1441 gtgatagagc acacgcacgc cgaagcctat gtctttaaca tctcgaatcg aagagattct
     1501 gcagtcttca ccttcgatat
//
LOCUS       DASTWE010000319         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1365942, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000319 DASTWE010000000
VERSION     DASTWE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365942"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..351)
                     /locus_tag="VFT30_03425"
                     /pseudo
     CDS             complement(130..351)
                     /locus_tag="VFT30_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT          452 a          548 c          523 g          409 t
ORIGIN      
        1 ctggaatctc tgcgaaagcg acggcaggta tcgatgagtg acccgcagtg gtcagcgtga
       61 agaacaacgg cctaaagctg atagcacagc ttaagagcgc ggaggtcgca ggactggctg
      121 atggcgatct cacccaccca tcaactcgcg aatccgctgt tccaaattgg ccatatcata
      181 gggtttgaag agaagccaac gcggcggtcc catgtatccg ctggctgctt tgacgttctc
      241 cacgccagat gcaagaagca ctttcacccg cggtttgtgt tgcctgagcc atcgctgcag
      301 ctcgaatcca tccattccgg gcatttgaac atctgaaaag accacgtcga catgtcgcct
      361 ttttctagga ggtcgattgc ctgcctagcg tcatgtgctt ccagagttcg atatcccgca
      421 tcacggagac agtcgcatgc aagcgagcat atgagtggct cgtcatcgac gaccagcact
      481 gtcggcgtac tctcgcggcc cgtgtcgttg cccatggccc gccttgcgat agtggcttaa
      541 atactctgcc cgcccgatag ttcccaagca aggatgagat tgcttccgca tgaaagcaag
      601 ctattctgaa tgccatggtt gagccagtca ctctcacttc gtttgggaat ggaagatatt
      661 tacttcgggc gtcttgcact cggggtgtcg agtgcaattc cacatgggag attgatatca
      721 atcaggcaat cgagtccttc ggagatataa cccttacgga attgcgtcga gactgccgct
      781 gcccaaaatg caatggacct attgtaacca ctctcatctc gatgaaatga cgcgcgagcg
      841 ctcgtcaaac cgcgaagcgt cttcaggagg tgagcgagcg gtcggacaag aagttctggt
      901 cggacgggaa cctgcccccg atcaatcagg cggcgtatag aactgaaacc gcaggagcgg
      961 cctgtcgaag gggtggacaa tagcgcggag ctgatctgga cggccgaagt tcagcagcat
     1021 ctgcgggcca caggtccgat gggcggcgtc caaagccgat gttgccagaa cctcacggtc
     1081 ctctccatca tcccaagcac cctttacaaa gcatctgacg caataagtct tcatagtctt
     1141 acgttgctga gcccgtcggc gagcggcttt gacttggatc aaccgatccg cctctagctc
     1201 ttacgaacta acttctggat aggcttgcat ctgcaatgtg aaagaagaat gcagcacgta
     1261 acgggtacgg aacacgaatt gcgcctacct gaataatcga tcccgccagt tccccagcgt
     1321 cggcgcctgc ttcgatcgcg gatacctttc tccaaagccg aatcatatcc tgtcaggaca
     1381 tgcgccctgc gctacgctat ctgcgcaaga cctctctccc gggcatgagg ggtagagcga
     1441 gatccagcac gcggcgtcga actctgttgc gccgccgagg gagcactccc aatagacgca
     1501 gggcagcgcg actatgactc cgacgctgtt tattcgttca atcaatctcc ggatagaacc
     1561 ccttttttct gaatgccgag cgtaacttca gcgcagtatt ctcgacgcaa cgccgacgga
     1621 gagcacggcg ccgttgtttg agctcgattc tgaaatgtag aatgttggac ggaccacctc
     1681 acgaaaagct cgggcagttc tcctgggaag aactgcccga gttcgttaaa cgaccatcgc
     1741 tcgacgagcc aaaagcgagc ccatgcatga aacaacctcc caccgaacgc gagcgtgcca
     1801 tcgcactgca acgtgctgaa ggacgaacgt tacagaggat cggaaaggag tttggcctaa
     1861 cgcccgaatc cgttcgggtc atctgtcggc gcgtggaaaa ttatgatcgt ggtgcagcca
     1921 tgctgcgcga ta
//
LOCUS       DASTWE010000320         1301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_978209, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000320 DASTWE010000000
VERSION     DASTWE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978209"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFT30_03430"
     CDS             <1..913
                     /locus_tag="VFT30_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU5091706.1"
                     /translation="ANIDAWPICVNTQEPDEIVRIVQGIATGFGGINLEDISAPRCFE
                     IENKLKQSLDVPVMHDDQHGTAVVLLAALTNALRVTGKQLEQVRIVVNGLGAAGTACC
                     RILLAAGAVHLLGCDKEGIILYGEAEQLRACRTDLRACLTRDSPKGTLHDALKGADVF
                     IGLSVGNVVTADDLNLMASDRIVFALANPDPEVSPEVGTAHSAVFATGRSDYPNQINN
                     ALAFPGIFRGALDVQASQINEAMKLAAAQAIAHVIPENTLSEDYIIPSVFDKEVVPRV
                     ARAVAAAARASGVARRRAKVDDPSTLE"
     gene            970..>1301
                     /locus_tag="VFT30_03435"
     CDS             970..>1301
                     /locus_tag="VFT30_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091707.1"
                     /translation="MAVVQCPFLEVRGTMPFTTAKFLKFRSGMQKRIGSLRSKTEDSL
                     EFAVDTMMEERVDGFFQVEQGLEEIIKSLVEIEEELEAIRDLAGAMRLESRLEFVEER
                     WDEFDSEIR"
BASE COUNT          290 a          321 c          399 g          291 t
ORIGIN      
        1 ggcgaatatc gatgcctggc cgatctgtgt gaatacgcaa gagccggacg aaatcgtgcg
       61 gatcgttcaa ggtatcgcga ctggattcgg gggcatcaat ttggaagata tcagtgcgcc
      121 tcgttgcttt gagatcgaga ataaacttaa acaatcactc gatgttcctg tgatgcatga
      181 cgaccagcat ggcacggccg tggtgctcct ggcagcattg acgaatgcgc tgagggtcac
      241 cggcaaacag ctggaacagg ttcggattgt cgtcaatggt ctcggcgccg caggcacggc
      301 gtgctgtcga attttgttgg ctgcaggtgc ggtacatttg ctgggatgcg acaaggaggg
      361 catcatcctg tacggggaag ccgaacaact acgggcctgc cgtaccgatc ttcgtgcctg
      421 tttgacgcga gacagtccga aaggaacgtt gcatgatgca ctgaaaggcg ccgatgtatt
      481 tatcggtctg tccgtcggca atgtggtgac ggctgacgat ctcaatctaa tggcttcaga
      541 tcgcatcgtc tttgcattgg ccaaccctga tcccgaggtt tccccggagg taggaaccgc
      601 tcattctgcg gtctttgcga ccgggcggtc ggattatccg aatcagatca acaatgcgct
      661 cgcgtttccc gggatctttc gaggtgcgct cgatgttcag gccagccaga tcaacgaggc
      721 catgaagtta gccgcagcgc aagccattgc ccacgtgatc ccggagaata cattaagcga
      781 ggattacatt atccccagcg tgtttgacaa ggaagttgtc ccgcgtgttg cgcgggccgt
      841 cgcggctgct gcgcgcgcca gcggagtggc cagaagacgg gccaaggtgg acgatccttc
      901 cactcttgaa taagtgccac atcgctccca gggaaataaa cagtctgctg cggccttcag
      961 gctgtcgctg tggctgtggt acaatgcccc ttcttggaag tccgagggac catgccgttc
     1021 accaccgcga aatttctgaa gttccgaagc gggatgcaga agcgcatcgg ttcactgcgt
     1081 tcaaagacgg aagacagcct ggaatttgcc gttgatacga tgatggaaga acgtgtcgac
     1141 gggttctttc aggtagaaca gggactcgag gaaatcatca agtcccttgt tgaaatcgag
     1201 gaagaattgg aggcgattcg agatttggct ggggccatgc gtttggagtc caggctggag
     1261 ttcgtcgaag agcgatggga tgaattcgac agcgaaattc g
//
LOCUS       DASTWE010000321         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_784424, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000321 DASTWE010000000
VERSION     DASTWE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784424"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFT30_03440"
     CDS             <1..439
                     /locus_tag="VFT30_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091708.1"
                     /translation="MMKPEKVVGIIVVAVLAIMQGCAAPQPSPDLLAPTEAQMKIRSM
                     QTRSFDVKDRQMAMRGVIAALQDLGFIIERANEPLGLVTAARFAEPNFYDVVGVTVTV
                     RTEAEGRMMIRANAIYNNKPIEDPKVYQNFFAALERSLFVTKN"
     gene            439..1056
                     /locus_tag="VFT30_03445"
     CDS             439..1056
                     /locus_tag="VFT30_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091709.1"
                     /translation="MDRVSHQRNRDEHIQPLAGSGRLSPWLFVGLCLLLQGCPTPYEL
                     RHVNQWDSREQIWLSEESQVKIRAAQSRVFDTVDRRRMLATIVSTLQDLNFKVEVLDE
                     ELGVVSGKKYVENEQIYGVDLSYLLYQPDALLVLNRNYRTWGPFDHRSNLVRFTVTVR
                     KRNDSQLVVRASAQFYLRAVEDPVPYQQFFRALEQAVFLESHLVE"
BASE COUNT          259 a          319 c          304 g          257 t
ORIGIN      
        1 catgatgaag ccagagaaag tggtaggaat catcgtggtc gccgtacttg ctatcatgca
       61 aggctgcgcc gccccgcagc ccagtccgga cctgttggcg ccgaccgagg cgcagatgaa
      121 gatccggagc atgcaaactc gatcatttga cgtgaaggat cgccaaatgg cgatgcgcgg
      181 ggtcattgca gccctacaag atttaggctt catcatagag cgggctaatg agcccttggg
      241 attggtgacg gctgcacgtt tcgcggagcc gaacttctac gatgtcgtgg gcgttacggt
      301 cactgtgcgc acagaggctg aaggccgtat gatgattcgc gctaatgcca tctacaacaa
      361 caagcccatc gaagacccca aggtgtacca gaatttcttc gccgcgctgg aacgctctct
      421 ctttgtcacc aagaattaat ggacagagtt tctcaccaga ggaaccgtga tgaacacatc
      481 cagcctctcg ccgggtcggg acggctgagc ccctggctct tcgtcggact ctgtcttcta
      541 ttgcagggat gcccaacgcc ctatgaatta cggcatgtca accagtggga ctcgcgcgag
      601 caaatctggc tttccgaaga aagccaagtt aagattcgcg ctgcccaatc gcgcgtattc
      661 gataccgtgg atcgtcgtcg gatgctcgct acgattgttt ccactctgca ggacttgaat
      721 ttcaaagtgg aggtgcttga tgaagaattg ggtgtcgtct ctgggaaaaa atatgtcgag
      781 aacgaacaga tctatggcgt tgacttgagc tatctactgt atcaacccga tgcattactc
      841 gtcctcaacc gtaattaccg gacatggggc ccgttcgatc accgcagcaa tctggtccgg
      901 ttcacagtca ccgtccgcaa gcgcaacgat tcgcagctcg tcgtacgggc cagtgctcag
      961 ttttaccttc gtgccgttga ggaccccgtt ccctaccaac agttcttccg cgccctggaa
     1021 caagcggtct tcctggaaag ccacctggtt gaataacctg cgaggtctgt gcggataact
     1081 tcatccgacg ggcgatccct cggtcgatta accctcccaa aacgacgctg aactgaatc
//
LOCUS       DASTWE010000322         1330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_137922, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000322 DASTWE010000000
VERSION     DASTWE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137922"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1267
                     /locus_tag="VFT30_03450"
     CDS             272..1267
                     /locus_tag="VFT30_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HEU5091710.1"
                     /translation="MKWTRSFVRRCLAVALLVAIGLNLYGCSDSASINPEVELSSLTV
                     APGTLQPSFAGTTTQYNVDLSNSVTNVTVTAQPAVSGDTVSIDGQTTTRRSINVGSAG
                     GPPTVVSVVVSESTSKSRTYTVVLNRASLAGNNDLSALTVAPGDLSPSFASDRQNYTV
                     DVATDVTGVSVSATKSDPNAVLSGDVPNQGQAIIPLGGPGTTKDLLITVTAQNGIAKT
                     YRITIKRAAPTNNNNLSALSMNAGSLSPPFAPGTVNYSVEVAASVDRIIISATKSDPN
                     AVMSALGSVIAPAGVSSGQVSATPGLGTIVPVPIIVTAPDGSQKTYTINVFRLSR"
BASE COUNT          315 a          389 c          338 g          288 t
ORIGIN      
        1 acttatcaca gcagtgagta gacggtccgc gcaacaatgg cttaccgtcc acgatggtga
       61 ttccctctcg tatcattaga accttcatat atcccgatag gatctgttga ccggattggt
      121 gagatcggtt atagaaggga ccactgctcg gcccttgcga aaatcctgac aggccgatta
      181 tgaaccctct cgtagcctct acacctaccg tttgggttca ccagaaaaaa atagattcat
      241 tgttcatatg ctaccaacga ggacaggcac cttgaaatgg acacgtagtt ttgtcagacg
      301 atgtttagcc gtcgcgcttc ttgtcgcgat cggtttgaat ctctacggct gctcagactc
      361 ggcgtcgatt aacccggaag tggaactcag cagtctgacg gtcgcacccg gaacgcttca
      421 accatccttt gccggtacaa caacccaata caacgtcgat ctttcgaaca gcgtcacgaa
      481 tgtgaccgtc actgctcaac ctgccgtatc cggcgacacg gtgagcatcg atggccagac
      541 gacgacgcgc cgctcaatca acgtaggctc ggcgggaggt cccccgacgg tcgtcagcgt
      601 tgtggtgtcg gaatcgacca gcaagtcccg gacgtatacc gtcgtcctca accgggcatc
      661 gttagcaggc aacaacgatc tgtcagcgtt gactgtggca cccggcgatt tatcaccctc
      721 atttgcttca gaccggcaga actacacggt ggatgttgcg accgatgtga ccggtgtgag
      781 cgtctccgcg accaaatccg atccgaatgc cgtgctatcc ggcgatgtgc ccaatcaagg
      841 tcaagcgatt atcccgctcg gtggtccagg aacaaccaaa gacctgttga ttacagtcac
      901 ggcacagaat ggaatcgcca agacttaccg catcaccatt aaacgagcgg cgcccacaaa
      961 caacaataat ctgtctgcat tgagcatgaa cgcaggcagt ttgtctccgc cctttgctcc
     1021 ggggacggtc aattactcag tggaggtcgc cgccagtgtc gaccgaataa tcatctctgc
     1081 aaccaagtcc gatccgaacg ccgtgatgtc cgccctcggt tcggtgatcg cgccggcggg
     1141 agtctcgtca ggtcaagtaa gtgctacacc ggggctaggg acgatcgttc ccgtgccgat
     1201 catcgtaacg gcaccagatg gaagccaaaa aacgtacacc atcaatgtgt tccgattgtc
     1261 ccgttagaca gagcgaatgt cgtttctggt cttgcggcac cctacctagc caagtacaag
     1321 tagccccacc
//
LOCUS       DASTWE010000323         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_331994, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000323 DASTWE010000000
VERSION     DASTWE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331994"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFT30_03455"
     CDS             <1..693
                     /locus_tag="VFT30_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091711.1"
                     /translation="PFADRSIDLVIDNENMADMTPVQLGKKELACGIGVTAQHQEALD
                     WIRRLRLPIERDPPESVIFNLGPIRFVAELWRVLKPGGRAFLTEFGIEDGWPAPVKLP
                     GHTEYEVQYSHLRQAVRWLGFQERYLTLPQFLGLKPDTKVLCTGATYTIQRFCQSINR
                     PFAVRAYTEKELQQTLGDMLPKLQGLHYHDLADPAWFGLQDFKVLLLEKPGGTPRAEF
                     TENKGYRWYSQK"
     gene            complement(705..>1090)
                     /locus_tag="VFT30_03460"
     CDS             complement(705..>1090)
                     /locus_tag="VFT30_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEU5091712.1"
                     /translation="CVEIDSSLVRLEEFRRLSREQFLSSQDALDVACYRLLIAIESAL
                     ALCFHVSAKRLHQVPEEYAGCFVALEQAGLIPGDLSCRLQQMARFRNLLVHVYWKIDY
                     GQVYDIIETRLDDLRAFRTAIAGLV"
BASE COUNT          282 a          307 c          287 g          214 t
ORIGIN      
        1 ccgtttgccg atcgttccat cgatctcgtg atcgacaatg agaacatggc agacatgaca
       61 cccgtacaac tgggcaaaaa agagctggcg tgcgggatcg gagtgacagc ccaacaccaa
      121 gaagcactgg attggatcag acggcttcgc cttcctatcg aacgcgatcc gcctgaatcg
      181 gtaatcttta acttaggacc gattcgcttc gtcgccgagt tgtggcgagt gctcaagccg
      241 ggtggccgag cgtttctcac cgagttcggc attgaggacg gttggcccgc acccgtgaag
      301 ctaccaggac atacggaata cgaggttcaa tacagccacc tgcggcaagc cgtacgctgg
      361 ctcggatttc aagagcgata tcttactctt ccgcaattcc tgggactcaa gccggacacg
      421 aaagttctct gcactggcgc aacctatacc atccaaagat tttgtcagtc gatcaaccgc
      481 cccttcgccg tgcgcgccta tacagagaag gagttgcaac agaccttggg tgacatgctc
      541 cccaaacttc aaggcctcca ctatcacgac ctcgccgatc ctgcgtggtt cggcctccag
      601 gacttcaaag tgctgttgct ggaaaaacca ggtgggacgc cgagggcgga attcaccgaa
      661 aacaaaggct atcggtggta ttcgcagaaa taggacgtga accgttaaac caatccggcg
      721 atggctgtac ggaacgcacg aagatcgtcg aggcgtgtct caatgatgtc atacacctgc
      781 ccgtaatcaa tcttccagta cacatgcacg agcagattcc tgaatcgcgc catctgctgt
      841 agacggcatg aaaggtctcc ggggatgagt ccggcttgct ccagagcgac gaaacaaccg
      901 gcatactcct ccggaacttg atgcaaccgc ttcgccgaga catgaaagca aagagccaat
      961 gccgactcaa tggcaatcag caatcgatag caggcaacat cgagagcatc ttggctggaa
     1021 agaaattgct cccgggagag acggcggaac tcttcgaggc gaaccaatga cgaatcgatt
     1081 tcaacgcatc
//
LOCUS       DASTWE010000324         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_461258, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000324 DASTWE010000000
VERSION     DASTWE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461258"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFT30_03465"
     CDS             complement(<1..604)
                     /locus_tag="VFT30_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091713.1"
                     /translation="MRRAGILTGGVVALILLAVVCIPGHLSQSRTTAVTQATLHASVE
                     QGSLVLRGSLPNERSKATILEQAHAFAAKTRMRVIDQFIVDKQVKAAVWVDTVPQLLP
                     ILGLMVERGSIIIDGRSLVVNGQVAGHREKAEVLQAVAPAIRAGLRIEDRVVVTPAAS
                     SSSAAVPLSALQLLLNQVLTKSSIEFEPKSATMTTKGQMVL"
BASE COUNT          230 a          281 c          272 g          218 t
ORIGIN      
        1 ccagcaccat ctgtcctttg gtcgtcatcg tggcactctt gggctcaaac tcaatcgagg
       61 atttcgtcag aacctgattc agcaacagct ggagtgccga caggggaacg gcggcggaag
      121 aagatgaggc cgccggcgtc accaccacac ggtcttcaat tcgtaagcct gctcgtatcg
      181 ccggagccac ggcctgtaat acctcagcct tctcgcgatg accggccacc tgaccgttga
      241 caaccagcga acgcccatcg atgatgatcg acccccgctc gaccatcaag cccaagatcg
      301 gcaagagttg tggaaccgtg tccacccaca cagccgcttt gacctgctta tcgacgataa
      361 actggtcgat cacgcgcatt cgcgttttcg cagcgaatgc atgagcctgc tcaagaatag
      421 tggccttact gcgttcattg ggcaacgaac cgcgaaggac caagctgccc tgttcgacgc
      481 tggcgtgaag ggtggcctga gtcacggccg tggtccgtga ctgcgagaga tgtcccggga
      541 tgcacaccac ggcgaggagt atcagcgcaa cgacaccgcc ggtaagaatc cccgcacgcc
      601 tcatgagatc agcccgctgt agatgatgtg attactggag tgcgacgcgg gcacggtacc
      661 acatttgata ataaatgtac tagggtaaga tttatcgttg aagcgggcgg ctgcacaggg
      721 ccttatctct gcgagaaggt tacaaaatga ccgcagtctc aggattcaga gtagacgatc
      781 ctgagtggcg tgtatcttct cagcatctct cgttataaga aggacggaac aaactggggc
      841 attcaccagt ctcttcacag aagtgagaac agtatgcttc ttgtatggtt tcgtccacag
      901 ctatcctaca cgaagacgag ctggataaac ttccccttcg ttggtgggcg atcacgctcc
      961 ggggattact cggtattctt ctcggtattg ctgcgttctt c
//
LOCUS       DASTWE010000325         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1075327, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000325 DASTWE010000000
VERSION     DASTWE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075327"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFT30_03470"
     CDS             complement(<1..894)
                     /locus_tag="VFT30_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5091714.1"
                     /translation="MGIGLALVCVIGLGLGVATASAQSSAQSDGQLYSLEMIVDLALA
                     RNPLVSFAEGNIEQQKGQQTAAGAYPNPTVSGSGGRGSLRDAGRAGIGPALDRDSLTE
                     SNMTIGQPLEWPAMRAARQQVADLGLATANVGMLETRLNLASQVKVAFYDLLLAQQGA
                     DLARQNLDTVEGVARIVKARVKSGEAPQFESIKAEVEVLKARQQLARADNLVRINRVA
                     VDTLTGGALGPSYMVYGEFRILPRDLQIEGLMARMMEQHPTIQRLLKSVEQSDWKIEF
                     ERQARVPTVTVNGSYWREIGRE"
     gene            complement(964..1800)
                     /locus_tag="VFT30_03475"
     CDS             complement(964..1800)
                     /locus_tag="VFT30_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5091715.1"
                     /translation="MQHLAALTRAQASLIRQLLRDKKTRSKEGAFVLEGTKACLDLIH
                     RHSQSILSLVVSPRFLCVEGEADRRVRMKLHARQFVCPDAGFEKLTDVEMPQGILAVV
                     RQPGWDEAKVFGRTSVLGIYGDRLQDPANVGAIIRTAAALNLSGLWLSPDSADHFSPK
                     VVRATAGTILSLPIFYTRNFQAFSSYGCEIYSAVLAAADIVPIRSIQTIPSRLMIAVG
                     NEGAGLAPDVVKVSNVRFSIPLAKGVESLNVAATAAISAFYFSGLRLDSDVGSMRSCH
                     SA"
     gene            complement(1874..>2110)
                     /locus_tag="VFT30_03480"
     CDS             complement(1874..>2110)
                     /locus_tag="VFT30_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091716.1"
                     /translation="ESLEDRIKRARNSGDWIQLSKAECASLHKREKAHLKSQLEQLQF
                     EQTRTRGKLTALKRAKARAQRIRAAEAASERRRR"
BASE COUNT          462 a          647 c          547 g          454 t
ORIGIN      
        1 ttctcggccg atctcgcgcc aataactgcc gttgacggtg acggtcggca cccgtgcttg
       61 ccgttcgaat tcaatcttcc agtccgattg ttctacggac ttcagcaagc gctggatggt
      121 cggatgctgt tccatcatgc gagccatgag tccttcaatc tgcaggtcac gagggagtat
      181 cctgaattca ccgtagacca tataagacgg cccaagcgca ccaccggtca gggtgtcgac
      241 ggcaacgcgg ttgatccgaa caagattatc cgcacgtgcg agttgctgtc gagccttcag
      301 gacttccacc tccgccttga tggattcaaa ttgaggcgct tcgcccgact tgaccctggc
      361 cttcacgatc cgagccacac cctcgacggt gtcaaggttc tggcgcgcga ggtcggctcc
      421 ctgttgggcc agcagcagat catagaaggc cactttgacc tgcgacgcca aatttagtcg
      481 ggtctccaac atgccgacat tggcggtcgc aagtcccaga tccgccactt gttgcctggc
      541 ggctcgcatg gccggccatt cgagcggttg accgatcgtc atattcgatt cggtaagcga
      601 gtctcgatca agagccggcc caatccccgc ccgacctgca tcgcgaaggg agccgcgccc
      661 tcctgaacca ctcacggtgg gattgggata ggcaccggct gccgtctgct gtcccttctg
      721 ctgttcgatg tttccttcgg cgaaggaaac caagggattt cttgccaacg ccagatcgac
      781 gatcatctcg agactgtaca gctgtccatc ggattgggca gaggattgag cagaggccgt
      841 ggccacacca aggccgagac cgatcacaca taccagcgcg agccctatcc ccattctcat
      901 ctcctgcccg acatcatgaa ctgcgagtca tagtaggccg aatgtattct atatttcaaa
      961 tctctatgcc gaatggcacg acctcatcga accaacatcg gaatcgagtc gcaatccgct
     1021 gaagtagaat gccgaaatcg ccgctgtcgc cgcaacattc aacgattcca cccctttggc
     1081 caatggaatg gaaaaccgga cattggacac cttcacgacg tcaggggcca atccagctcc
     1141 ttcgtttccg acagcaatca tgagccgact gggtatcgtc tgaatgctcc tgatagggac
     1201 tatatcagcc gcagcaagga ccgccgaata gatttcgcat ccatacgagg agaatgcctg
     1261 aaagttccga gtataaaaga tcggaaggct caggatggta ccggccgtag cacggacaac
     1321 cttggggctg aaatgatcgg cggaatcggg gcttaaccaa agtccggaca ggttcaaggc
     1381 tgcggcggtc cggataatcg ctccgacatt cgccggatct tgtaaccgat ccccgtaaat
     1441 acccaacacg ctcgttcgcc cgaacacctt agcctcgtcc cacccaggtt gtcggacgac
     1501 ggccaagatc ccttgcggca tttccacgtc ggtcagcttt tcaaagccgg cgtctggaca
     1561 aacaaactgg cgcgcatgca gtttcatgcg cacccttcgg tctgcctcgc cctcgacaca
     1621 gaggaaacgc ggcgatacga ctaggctgag gatagactgt gagtgccgat gaatcagatc
     1681 aaggcaggcc ttcgtccctt caaggacaaa ggctccttct ttcgatctgg tcttcttatc
     1741 gcggagcagt tgccggatca gcgaggcttg cgcgcgagtg agagccgcca aatgctgcac
     1801 tgcacccact atactcttaa acccctgtca tcgaactgaa taaaacacta ctcaaaccgg
     1861 tcgcggcagt cgattaccgg cgccttcgtt cggaggccgc ttccgcagca cggatacgct
     1921 gcgccctcgc cttggctcgc ttcagtgcag tcaacttacc gcgagtccga gtttgctcaa
     1981 actgcaactg ttcaagctga gacttcaagt gggctttttc ccgcttatgc agacttgcgc
     2041 attccgcctt ggacaactgt atccagtccc cgctattcct ggcgcgcttg attcgatcct
     2101 ccaaggattc
//
LOCUS       DASTWE010000326         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_752317, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000326 DASTWE010000000
VERSION     DASTWE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752317"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFT30_03485"
     CDS             <1..220
                     /locus_tag="VFT30_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091717.1"
                     /translation="DGVVITHVRHRVSLERAGTSLQEALISIERGIEPEFVAVDLRAA
                     ADALGEIVGAITSDEVLDRIFSEFCIGK"
     gene            229..>1657
                     /gene="mnmG"
                     /locus_tag="VFT30_03490"
     CDS             229..>1657
                     /gene="mnmG"
                     /locus_tag="VFT30_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU5091718.1"
                     /translation="MTTAFDVIVVGGGHAGCEAALAAARMGAKTLLLTMELSRIAQMS
                     CNPAVGGIAKGHLVKEIDALGGEMGRNTDRSGIQFRFINTSKGPAVRALRAQCDKSLY
                     RMAMQETLVSQDHLTLAEGVVDRLLVSGGTVTGIVTGSGETIDAKAVILTSGTFLKGL
                     IHIGLNHFPAGRAGEASAEHLSDCMRDLGFDVGRLKTGTPPRLDKATIDFSKMTPQPG
                     DTPPPPFSYRTQHITTRQVLCHLTYTSRETHDIIQENLDRSPLYSGVIESTGPRYCPS
                     IEDKVVRFAEKERHQIFIEPEGLDSIEFYPNGISTSLPVDVQAAMLKTIPGLEDAKIV
                     KPGYAIEYDYFPPRQLYRTLETKRVPGLYHAGQINGTSGYEEAAAQGLVAGINAVLKL
                     LEKPPLILDRSQAYIGVLIDDLITKDAYEPYRMFTSRAEYRLLLRHNNADLRLMPVGY
                     EVGLIPEEAYKKFLRKKCLIEKEIEH"
BASE COUNT          398 a          443 c          442 g          374 t
ORIGIN      
        1 cgatggtgtt gttatcacac atgttcgaca tcgggtgtcg ctagagcgtg ctgggacatc
       61 actccaagag gcattgatct cgattgagcg aggtatagag ccagaatttg tggcggtcga
      121 cctccgagca gcagccgatg cgctgggaga gatcgtcgga gcaattacgt ccgacgaagt
      181 tttagaccgc attttttccg agttttgtat cggtaaatag atgaaccagt gaccaccgca
      241 tttgacgtca tcgtcgtagg gggcggccat gccggctgcg aagcagcgct agctgcggct
      301 cgaatgggtg cgaaaacttt gcttttgacg atggaactga gccgcatcgc acagatgtcg
      361 tgcaatccgg cagtcggcgg aatcgccaag ggacacctcg tgaaggaaat cgatgcgctc
      421 ggcggtgaga tgggacggaa cacggatcgc tccggaattc aatttagatt catcaacaca
      481 agcaaaggtc cggcggtaag ggcgttgcgc gcccaatgcg acaaatcgct gtatcgcatg
      541 gctatgcagg agaccctcgt ctcgcaggat caccttaccc tggcggaagg agtcgtggat
      601 cgactgctcg tgtccggagg gacggtaacc gggattgtga cgggctcggg tgagacaatc
      661 gatgccaaag ccgtcatact gacatcaggt actttcctta agggacttat ccatatcgga
      721 ttgaaccatt tcccggccgg tcgcgcggga gaagcctcgg ctgaacacct ttcggactgc
      781 atgcgtgacc ttggatttga tgtcggacgg ctcaagaccg gaacccctcc ccgattggac
      841 aaggctacga ttgatttctc gaagatgact cctcagcccg gcgatactcc tcctccccct
      901 ttttcgtatc gcacccaaca catcactact agacaagttc tctgtcactt gacctatacc
      961 agccgagaga cacacgacat cattcaagag aatcttgacc gctcgcccct gtacagcggg
     1021 gttatcgaat ctaccggacc tcggtactgc ccttccatcg aggataaggt cgtgcgtttt
     1081 gccgagaaag aacgtcacca gatttttatc gagcctgagg gcctcgactc gatcgaattt
     1141 tacccgaacg gcatttcaac cagcctgccg gtcgatgttc aggccgctat gctgaaaacg
     1201 atccctggat tagaggatgc caaaatcgtc aaaccgggct atgcaatcga gtacgattat
     1261 ttccctcctc gtcagctcta tcgaacgctt gaaaccaaac gggttccagg tctttatcac
     1321 gccggacaga tcaacgggac ttccgggtat gaagaagcag ctgcgcaggg tttagttgcc
     1381 ggtattaatg ccgttctgaa gttgctggag aagcctcccc tcatcctcga ccgatcccaa
     1441 gcctacatcg gggtactcat cgatgatctc attacgaaag atgcctacga gccttatcga
     1501 atgttcacgt caagagcaga atatcgattg cttctccgcc acaacaacgc agaccttcga
     1561 ctcatgccgg ttgggtacga ggttggcctg attcccgaag aagcttacaa aaagttttta
     1621 cggaagaaat gtctgattga aaaagagatc gagcacc
//
LOCUS       DASTWE010000327         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_105752, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000327 DASTWE010000000
VERSION     DASTWE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105752"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFT30_03495"
     CDS             complement(<1..977)
                     /locus_tag="VFT30_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091719.1"
                     /translation="MDRVSGQVTSPGGLNSSGSVFLVNHNADNSLVTFRYRLKDVEME
                     SAEEPFEAGGRKFNRGSFIIRKGTDEIKRVANELGIQVQAVADAPSVKTHPVRAARIA
                     VMHTWLSTQDEGWYRVGLDQNQVPFAYISTQDAAKDANLKSKYDVILFAPVGRGSQAI
                     INGIPMYGNPIPWKVTPLTPNLGRTDETDDIRPGLGWSGLQNLQKFVRDGGVFITVDD
                     TSDFAVNYGFTAGVSVSRSSRLRAVGVILRTKTVDAASPIVYGYGDSLAMYCANGPVF
                     GINNGIGGRQRPQGRATGRGTPDDPDVPQGRPVAEIPDPPARVELWEAPP"
BASE COUNT          224 a          293 c          256 g          233 t
ORIGIN      
        1 ggtggagctt cccataactc gacgcgcgca ggtggatccg gaatctctgc aactggacga
       61 ccctgcggaa catctggatc atctggagta ccgcgaccgg ttgctcttcc ctggggacgc
      121 tgccgtccac cgatcccgtt gttgatgccg aacactggtc catttgcgca atacatggcc
      181 agggagtcgc cgtagccgta aacaatgggg ctcgcagcgt caacagtctt ggttcgtaga
      241 atcacaccga cggcacgtag acgggaggat cgactcaccg aaacacctgc ggtaaatcca
      301 taattgactg cgaaatccga cgtgtcatca acggtaatga aaaccccacc gtcacgtacg
      361 aacttctgca gattctgtaa accggaccac ccaagccccg gacgaatgtc gtcggtctca
      421 tcagtgcgtc cgaggtttgg agtcaagggc gttactttcc acggaatcgg attgccgtac
      481 atcggtatac cgttgatgat cgcctgtgaa ccacgcccca ccggcgcaaa caggatcacg
      541 tcatacttgc tcttcaagtt tgcatctttg gcagcatctt gtgtgctgat gtaggcaaac
      601 ggtacctggt tttggtccag gccaacccga taccaaccct catcctgggt acttagccac
      661 gtgtgcatta cggctatgcg agctgcgcga acaggatgtg tcttcaccga gggtgcatcc
      721 gccacggcct ggacctgtat gcctaactca ttggcgactc gcttgatctc gtctgtgcct
      781 ttgcgaatga tgaaagaacc ccggttgaac tttcggcccc cggcttcaaa cggctcttca
      841 gcgctctcca tttcgacatc cttcaagcga taacggaatg tcaccagtga attgtcagca
      901 ttgtggttta caaggaagac tgagccacta ctgttaagcc cgcctggtga cgtgacctgg
      961 ccgcttactc tatccattgc agcttgaagt accttcacat cagtaa
//
LOCUS       DASTWE010000328         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1398678, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000328 DASTWE010000000
VERSION     DASTWE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398678"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /gene="mltG"
                     /locus_tag="VFT30_03500"
     CDS             <1..379
                     /gene="mltG"
                     /locus_tag="VFT30_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5091720.1"
                     /translation="NRLKKHIPLQSDPTVIYGLPNFDGNLHKKDLTHPSPYNTYRWAG
                     LPPGPIANPGAQSIRAALYPLPSAYLYFVSKNDGTHQFSATLVEHNKAVEKYQKRPFR
                     RGNHSQTFMTPDGVQTHHKEGVS"
     gene            383..1060
                     /gene="deoC"
                     /locus_tag="VFT30_03505"
     CDS             383..1060
                     /gene="deoC"
                     /locus_tag="VFT30_03505"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5091721.1"
                     /translation="MSRWNLPVLIDHTALKPDATKVEVLRLCKEAKANGYTVIFVPPC
                     YIDEAVAAVAGTGIRVGIPIGFPLGGHSTKTKVAEAVEAVERGATVLDMVLNISRLKS
                     ADHDYVRTDIAEVVASTGGVEHKVILETCYLTHEEKRTACHLVVEAGADYVKTSTGFG
                     AGGATVEDVRLLNEVVAGRAKVKASGGIRDWKTTLAMLEAGADRIGTSASLKIIQEWR
                     TAIHKQE"
     gene            1245..2402
                     /locus_tag="VFT30_03510"
     CDS             1245..2402
                     /locus_tag="VFT30_03510"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU5091722.1"
                     /translation="MINRVILFVIDGFGIGALPDGADYGDADANTLTHLAETVDGLSL
                     PNFEMLGLGHIAPIKGVRAMVQPNGSFGRLGFASPGKDSVVGYWEIGGVIQKEVPTVC
                     RSGVPTKIVDVVEQLFGRKSIGRGVLSLGVMLRRHSMEHMATGSPILWTDGGNTCFLA
                     MHESIMTPAEFQQRCREIRKAVKDAGTIVRIVGQPVIGGHDLLRPQVGRRDFVIEPPG
                     LTMLDVLSRSGQIAMGVGKVYDLFSGRGLTKSFPAASGMAAFDEVIGMLNKVPRGLIC
                     ASLDLLSEDAVQSATAVQEFDRRLPDLFDKLRLGDMVLVTGDHGRDFSLPGRTSTREY
                     VPVFATGPKLAQGVDLGSRSTAADVGQTIVEALQAERLPVGESFLDALRPG"
     gene            2456..>2896
                     /locus_tag="VFT30_03515"
     CDS             2456..>2896
                     /locus_tag="VFT30_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5091723.1"
                     /translation="MSYEKKLSDLGLTLPTPPKPLANYVPVVRTGELLFLSGVLPSRD
                     GRLVMTGKLGENLTVEQGVEAAREAVLNGLSIIRSEAGSLDRVKQIVKMVGYIASAPG
                     FTDLPQVLNGASDLLVSVFGEAGRHARVAVGAAELPRLAPIEIEL"
BASE COUNT          651 a          722 c          882 g          641 t
ORIGIN      
        1 taatcggctg aagaaacata ttcccctgca gagcgatccg acagtgatct acggtctacc
       61 gaatttcgat ggaaaccttc acaaaaagga tctcacccac cccagtccct acaataccta
      121 ccggtgggcc ggtctgccgc ctgggccgat cgccaacccc ggggcgcagt cgatccgtgc
      181 cgctttgtat ccactgcctt ctgcctatct ctacttcgtc tcgaagaacg acggaacaca
      241 tcaattttcc gcgaccctcg tagaacataa caaggcggtg gaaaagtacc agaaacgccc
      301 cttccgacga ggaaatcact cgcagacctt tatgactccc gatggcgtac agacgcacca
      361 caaggaagga gtatcgtgaa gcatgtcaag atggaatctc ccggttctta tcgaccatac
      421 ggcactgaag ccggatgcga cgaaagttga agtactgagg ttgtgtaaag aagcgaaggc
      481 caacggttac acggtcattt ttgtcccccc atgttacatc gatgaggcgg tggcggcggt
      541 ggccggcacc ggcattcgtg tcggcattcc catcgggttt ccgttgggag gacacagcac
      601 aaagaccaag gtggccgaag cagtcgaagc agtggaacgg ggtgcaacag tgttggacat
      661 ggtgttgaat atcagcagac tgaagtcggc cgatcatgat tatgtgcgaa cggacatcgc
      721 tgaagtcgtg gcgtctacgg ggggcgtcga gcataaagtg atcctagaaa cttgttatct
      781 cacgcatgag gagaaacgaa cggcgtgtca tttggtcgtg gaagccgggg cggactatgt
      841 gaaaacgtcg accggtttcg gagctggggg ggcgacagtg gaagatgtac ggttgttaaa
      901 tgaagttgtc gcgggacggg caaaggtgaa ggcttcgggc ggcatccgcg attggaaaac
      961 cacgcttgca atgctggaag ccggagccga taggattgga accagcgcca gcctcaaaat
     1021 catccaagaa tggcggacgg caatacacaa acaagaataa agcagtgcgg agtttgggag
     1081 tggatttcgt tgctcagcac aacgaccgaa agcgtttgta ccggtatcaa tggcgtgctc
     1141 gtgcggcata acagacggtg atctcctgcc taggtcctcc tagatccaca tgtctgtccc
     1201 atacgcatct atgtcggact acggtgattt gttatagtgc gctgatgatc aatcgagtca
     1261 ttcttttcgt catcgacggg tttggaattg gggcattgcc ggatggagcc gactatggcg
     1321 atgccgacgc gaacaccctc acgcatctgg ccgagacggt cgatggactg agtttgccta
     1381 atttcgagat gcttggcctg gggcatatcg ctccgatcaa gggcgtccgg gcgatggtcc
     1441 agccgaacgg ttccttcggg cgactgggtt tcgcttcgcc gggtaaagat tctgtcgtcg
     1501 gatactggga aatcggcggg gtcatccaga aagaagttcc cacggtctgc agatccggcg
     1561 ttcccaccaa gatcgtcgac gtcgttgaac agctcttcgg cagaaagtcg atcggccgag
     1621 gcgtcttgtc cctgggggtg atgctccgcc gacatagtat ggagcatatg gccaccggat
     1681 cgcctattct gtggaccgac ggggggaata cgtgcttttt ggccatgcat gaatccatca
     1741 tgacaccggc ggaattccaa cagcgatgtc gtgagatccg gaaagccgtg aaggatgcgg
     1801 gaactatcgt tcgtatcgtc gggcagccgg tcatcggcgg gcatgacttg cttcggcccc
     1861 aggttgggcg aagggacttt gtgattgagc cacctggttt gacgatgttg gatgtcttaa
     1921 gtcgatccgg ccagatcgcg atgggggtcg gaaaggtcta tgatctcttt agtgggcgtg
     1981 ggttgacgaa atccttcccg gctgcgtcgg gaatggcggc ttttgacgaa gtcatcggca
     2041 tgttgaataa ggtgcctcgc gggctcatct gtgccagcct ggatttgctc tctgaagatg
     2101 ccgtacagtc agcgacagcc gtacaagaat ttgatcgtcg tcttcccgat ttgttcgata
     2161 agctgcggct cggcgatatg gtacttgtga ctggtgatca tgggagagat ttctcattac
     2221 caggacggac atccacgcga gagtatgttc cggtcttcgc gaccggccca aaactcgccc
     2281 aaggcgtcga tttgggaagc cgatcgaccg ctgccgatgt gggacagacg attgtggagg
     2341 cacttcaagc cgagcgatta ccggttggtg agagtttctt ggatgcgctc aggccgggat
     2401 aggcaggagt gaagggtgac gagttggcct gaaatgtcta gaaggagacg acagtgtgtc
     2461 ctatgagaaa aaactaagtg atcttggatt gaccctgccg actcctccaa agcctctcgc
     2521 aaactatgtg ccggtagtca ggacgggtga actactgttt ctctccggtg tattaccttc
     2581 gcgcgatggc cgtcttgtta tgacgggcaa gctcggagag aatctcacgg tcgaacaggg
     2641 agttgaggca gcgagagagg cggtactgaa tggcttgagt atcatccgga gcgaagccgg
     2701 atcactagat cgggtcaagc agatcgtcaa gatggtcggc tatatcgcct cagctcccgg
     2761 ttttaccgat ctaccgcaag ttctcaacgg tgcgtccgat ctgctcgttt cggtgttcgg
     2821 agaagcgggg cgccatgcac gagtagccgt cggtgccgca gagttgccac gcctggctcc
     2881 catcgaaatc gaattg
//
LOCUS       DASTWE010000329         1439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1010817, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000329 DASTWE010000000
VERSION     DASTWE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010817"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..1047
                     /locus_tag="VFT30_03520"
     CDS             598..1047
                     /locus_tag="VFT30_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091724.1"
                     /translation="MKEYRVVLSSLFLMLVACQSLPGFSRSGEVKDIAIRDRLSVTAI
                     QVTGGDEIRWTNKLGTPVRITFSDYVLNKLSCRSNNFSGHFYSGADAALQPNESASLC
                     FDKPGTIRYVIRREPNNEEIAESGQVQVGAFSGHPFTQNEPSVVAPP"
     gene            1202..>1439
                     /locus_tag="VFT30_03525"
     CDS             1202..>1439
                     /locus_tag="VFT30_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091725.1"
                     /translation="MQLSWMMLALLLTGCSDNTSTGSDDAHAPDAKPQAGIVRLTTEE
                     IQSGEIVVRSVTSGDFRVYREFPATVTPNHHATAQ"
BASE COUNT          337 a          410 c          361 g          331 t
ORIGIN      
        1 ggatcggtga cgatgcccca ccctccacac ctaacccctg ctgaccgagt tgctgactcg
       61 tttctccccc cgcaacccct tgtttgtctg cgcctatcgc gaccccatgc cctgagacgc
      121 cgatgatgcc gacgacggca cagatgactg caagcattct ctttccctga ttcgccatcg
      181 cttccattat tgccctgatc ggttccatga attaatttcc tccctgtagc acgttcgtgc
      241 gcctacgcga ttcctttgtc gggtgaccat ggatcatgac aatcgaggat gcgtatccca
      301 ttgggatgca ctcggcggtg cctacgaagg tagtacacac ggtgatcagt tgtgaatgcc
      361 caacatcctg gggctagcaa tcggacaaaa acgattccct cctccactga agcgcatttc
      421 ccatcgcgtg ctcttaaggc atacctttca tcaatcatgc tttttacctt acccgacgtg
      481 gcattgatca ttaactagga gaccgcctag gtttgcgtgg gtcggcatgc tcgggatgct
      541 ccgagtgagc cttgggtgat gtctatgcca tgctgaaaat tgggaggatc atcaccgatg
      601 aaagaatacc gagtcgtact ttcatcgctt ttcctgatgc tcgtggcctg ccaaagcttg
      661 ccaggcttct cccgcagcgg cgaagtgaaa gatatcgcca tcagagatcg gttgtcggtc
      721 actgccattc aagtgaccgg cggagatgaa atacgctgga caaataagtt ggggacgccg
      781 gtgcggatca ctttttccga ctatgtattg aacaagttat cgtgccgcag caacaacttt
      841 agcggacact tttatagtgg agcggacgcc gctctccaac caaacgaaag cgccagccta
      901 tgttttgaca aaccggggac gattcgctac gtgatccgaa gggaacccaa caacgaagaa
      961 attgccgaat ccggacaggt tcaggtcgga gcgttttcag gacatccatt tacacagaac
     1021 gaaccttccg tcgttgcgcc accgtagtac ggaaggaatg accgatgctt cgacgaacag
     1081 aacatggtcg ccataacagt acaggaatgc ctgtgaacat caccgggcgt cttgatttca
     1141 tgggtaagat aaacgccaca gcaagcggcg gcctcacatt tcctttctaa cggcaacgat
     1201 cgtgcaactc tcttggatga tgctggcgtt gctgctcaca gggtgtagtg acaacacatc
     1261 caccggctca gacgatgccc atgctcctga cgccaaaccg caagccggca tcgtccgcct
     1321 gacaactgag gaaatacaga gcggcgagat cgtcgtgcgc tcagtcacga gcggtgattt
     1381 cagggtgtat cgtgagtttc ccgcaacggt aactcccaat catcatgcta cggcccaaa
//
LOCUS       DASTWE010000330         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_105799, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000330 DASTWE010000000
VERSION     DASTWE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105799"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..435
                     /locus_tag="VFT30_03530"
     CDS             49..435
                     /locus_tag="VFT30_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091726.1"
                     /translation="MGQPIVFSGHVIGLLKEYMRDLVDQATQEQHSQEQFGFTPVPYR
                     PDQAFSDLLALLDDRIESEGIQVGLPESFLHDMWSLCNEAVEPISTRIWLEGNLDGRS
                     TTKARTRELTYHALIEFIESRSRERN"
     gene            439..>1058
                     /locus_tag="VFT30_03535"
     CDS             439..>1058
                     /locus_tag="VFT30_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5091727.1"
                     /translation="MPPWLSWGLILFQVYSLHVGTGWADPPKNTDERRIVTLVPVVTE
                     GLENPLFLTQAGDGTGRLFIVEQPGRIRVLEGLRLLPASFLDITKQVLSGGERGLLGL
                     AFHPNYRRNGRFFINYTRKPDGATVLAEYRKGATPTSASQEERILLIVPQPYPNHNGG
                     MVAFGPDGYLYVGLGDGGSKGDPDNRAQNLEDLLGKILRIDVDRGDP"
BASE COUNT          248 a          293 c          277 g          240 t
ORIGIN      
        1 agggtctgcc gatcttgctc cttccataaa gaagcttgat aatagcgcat gggacaaccc
       61 atcgtgtttt ccggccacgt catcggtctg ctcaaagagt acatgcggga cctagtggat
      121 caagcaacgc aagagcagca ttcccaagaa cagttcggct ttactcccgt gccttaccgt
      181 cccgatcaag ccttttccga cctcctcgcc ctgctggacg atcgcatcga atctgaaggc
      241 atccaagtag gcttgccgga atctttcctc catgacatgt ggagtctctg taacgaagca
      301 gttgaaccca tttctactcg catctggctg gaaggaaacc ttgatggtcg gagcacgacc
      361 aaagctcgaa caagggagct gacctaccat gcactgatcg agtttattga gtcccgttca
      421 cgagaaagaa attaaggcat gcctccctgg ttatcatggg gactcattct gttccaagtc
      481 tacagcctgc acgtcggcac agggtgggcc gacccgccaa agaatacaga cgaacgacgt
      541 atcgtcacat tagttccggt cgtgaccgaa ggccttgaga atccactctt tctcacacaa
      601 gccggagatg ggaccgggcg actcttcatt gtcgagcagc ctggtcgcat tcgtgtgctg
      661 gaaggactca ggctacttcc ggcttcattt ctcgacatca caaaacaggt tctgtccggt
      721 ggagaacgcg gtctgttggg gctcgcgttt catccgaact atcgccgcaa cggacggttc
      781 ttcatcaatt acacgaggaa accggatggc gcgactgtat tggccgagta ccgtaaggga
      841 gcaactccaa cgtcagcttc tcaggaagaa cgaattttgc tgatcgtgcc gcaaccctat
      901 cccaatcaca atggcggaat ggttgccttt gggcccgacg gctacttgta cgtcggcctc
      961 ggcgatggtg gatcaaaagg cgatcccgac aaccgtgcgc aaaatcttga ggacttgctc
     1021 ggcaagattc tgcggatcga tgtggatcga ggtgatcc
//
LOCUS       DASTWE010000331         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_881424, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000331 DASTWE010000000
VERSION     DASTWE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881424"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..738)
                     /locus_tag="VFT30_03540"
     CDS             complement(244..738)
                     /locus_tag="VFT30_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5091728.1"
                     /translation="MMRHLKKAPTSKVREKPTVSPCLSKVPRHRLVELLWSFTPAYQR
                     WSESLLVEKGLSPQRLRILGSIHERGPRIMSELKKELGVTATNVTALVDSLEKDGLVK
                     RKQHPTDRRATVIELTDKVMTELSPRCTEYKEQVAELFSDLSERECKAFVTTLEKLWR
                     RLQK"
     gene            886..2193
                     /locus_tag="VFT30_03545"
     CDS             886..2193
                     /locus_tag="VFT30_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5091729.1"
                     /translation="MDAISNSQNFDDILDLDLPFKKNDSAKNTFSFKFVERLEVIASP
                     TVRCMMSNLQPWLLTLLLILTGCTDQQANSGSQPPPPEVSVAQVLSKSVQQWDEYTGR
                     VSAIDTVELRPRVSGYVQRVAYKEGQDVKQGDLMFQIDQRPYRASLDNAQAQLTRARA
                     TEQLETIRNQRAQFLINDEAISQEELDLRRAAHAQSTADVHAAEAAVATAKLNLSFTE
                     VRAPVAGRASRALLTVGNLATADETLLTTIVSQDPMYVYFDADENSYLRYKEQERKNE
                     RTARDNAVHVGLANEDGYPHAGTVDFLDNQVNPTIGTVRARAVLPNVDRIFTPGLYAR
                     VQFMSRQKAEALLIDDKAVLTDQDRKYVYVVDKDGKAQRKDIVLGRMVEGLRVVQSGL
                     THADRIVVVGLQKIFYPGMPVVASEVPMGAPPAQALSTDMAEK"
     gene            2207..>2801
                     /locus_tag="VFT30_03550"
     CDS             2207..>2801
                     /locus_tag="VFT30_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5091730.1"
                     /translation="MNFSKFFIDRPIFAAVLSIVIFAAGLIAIPLLPISEYPEVVPPA
                     VIVRATYPGANPKVIAETVSTPLEEQINGVENMMYMKSVAGSDGVLQMTITFRPGTDP
                     DAAQVQVQNRVSQALSRLPSEVVNLGVTTQKQSPTFLVMVQLLSPDGKYDALYLRNYA
                     NIKVKDELARLPGVGQVQIFGSGDYAMRIWLNPDAIAS"
BASE COUNT          643 a          797 c          766 g          595 t
ORIGIN      
        1 atgacgctta ctcatcggtc attcaagcag cgagctaaag gggcagcaac aaacggaaga
       61 gagattcttc aggggccagg agccaagggt gatcgttctt tcattcaatc cagacgaatc
      121 catcagtgcg aaagacatac atccggccgg cgccataagg cttcatatct caacgtcagg
      181 atactatgcc atcggtcacg tcgcctgtta tcgttaagaa aatgtgagcc cgataggaag
      241 gggttacttc tgcaagcggc gccagagttt ctcaagagtg gttacaaagg ctttgcattc
      301 tcgctcgctg agatcggaga acagctcggc gacctgttcc ttgtattccg tgcagcgcgg
      361 ggaaagctcg gtcatgactt tgtcggtaag ctcgatgacg gtcgcgcggc gatcggtcgg
      421 gtgctgtttc cgtttgacca atccgtcttt ttcgagcgag tcgacgagcg ccgtcacatt
      481 cgtcgcggtc acgccgagtt cttttttgag ctcgctcatg atccgtgggc cgcgttcatg
      541 aatcgagccg agaatgcgca gccgttgagg ggacaacccc ttttcgacca ataaagattc
      601 cgaccatcgc tgataggctg gggtaaaact ccataacagt tcgaccagtc ggtgccgcgg
      661 cactttcgag aggcagggag acacggtcgg tttctccctg accttcgacg tcggggcttt
      721 cttgagatgt cgcatcatgg cgtcacggta cttcaaactc cataaatatt caatagtaaa
      781 ttattcgtaa attcataatt catatattga ctatttttca tgggatgccg gatactacat
      841 cataaggaac ccatgtgccg gcatcttgag caactatcgt cagctatgga cgccatcagc
      901 aactcccaga acttcgacga cattcttgat ctagatctcc cattcaagaa gaatgacagt
      961 gccaagaata ctttctcatt caagttcgta gagcggcttg aggtcatcgc ttctccaacc
     1021 gtaaggtgca tgatgagcaa tctccaacca tggctcctga cactattgct gatcctgact
     1081 ggctgcaccg atcaacaagc taacagcggt agtcagccac caccgccaga agttagtgtc
     1141 gcacaggtac tctcgaaatc ggtacaacaa tgggacgaat acaccggccg cgtgagtgcg
     1201 atcgacacag tcgaattgcg gccacgtgtc agcggttacg tgcagcgggt ggcttacaag
     1261 gaaggccagg acgtgaagca gggtgattta atgtttcaga tcgaccagcg cccctatcgc
     1321 gcatccttgg acaacgctca ggcacaactg acgcgcgcac gtgcaacaga acagctggag
     1381 accatacgca accagcgcgc ccaattcctc attaacgatg aggcgatctc gcaggaggag
     1441 ctcgatctgc gccgcgctgc gcatgcgcag agcacggccg atgttcacgc cgccgaagcc
     1501 gcagtggcca ccgccaaact gaacctgtcc tttaccgagg tccgcgcgcc ggtcgccggt
     1561 cgggccagcc gagctctgtt gacagtcggc aatctggcca cagccgatga gaccttgctc
     1621 acgacgatcg tctcgcagga tccgatgtac gtctatttcg atgccgacga gaacagttac
     1681 ttgcgctata aggaacagga acgcaagaac gagcgcacag cgcgggacaa cgccgtccac
     1741 gtcggactcg ccaacgaaga cggctatccg catgccggca cggtcgattt cctcgacaat
     1801 caggtgaatc ccaccattgg aacggtgcgc gctcgcgctg tgttgcccaa tgtcgaccgc
     1861 atctttaccc ccgggctcta cgcgcgcgtg caattcatga gtagacaaaa ggccgaggcg
     1921 ctcttaatcg atgataaagc cgtcctcacc gaccaggacc gcaaatacgt ctatgtcgtg
     1981 gacaaggacg gcaaggcgca acgcaaggac atcgtgctcg gtcgcatggt ggaagggctg
     2041 cgcgtagtgc aatccggact gacccatgct gacaggatcg ttgtggtcgg cctgcaaaag
     2101 atcttttacc ccggcatgcc ggttgtggcc agcgaggttc cgatgggcgc gcctccggca
     2161 caggccctat ctacggacat ggctgagaaa tagggaaaac ccgcccgtga acttttccaa
     2221 gttcttcatt gatcgaccga tcttcgccgc cgtgctgtcg atcgtgatct ttgctgccgg
     2281 cctcattgca atcccgcttc tcccaatcag tgaatatccg gaagtggttc ctcctgccgt
     2341 gatcgtgcgc gcaacctatc caggcgccaa ccccaaggtg attgccgaaa ccgtgtcgac
     2401 gccgctggaa gagcagatca acggtgttga gaacatgatg tacatgaaat cggtcgcagg
     2461 ctctgacggc gtattacaga tgacgatcac cttccgtccc ggtaccgatc ccgacgccgc
     2521 ccaggtgcaa gtgcagaacc gcgtcagtca ggcgctgtcg cggctgccgt cggaagtggt
     2581 gaatctaggt gtaaccactc agaagcaatc gccgacgttc ctcgtaatgg tgcagttgct
     2641 ctcgccggac gggaagtatg atgcgctcta cttgcgcaac tacgccaaca tcaaagtcaa
     2701 agatgagctg gcgcggttgc cgggcgtcgg tcaggtccag attttcggca gtggcgacta
     2761 tgcgatgcgt atctggctca accctgacgc gatcgcttcg c
//
LOCUS       DASTWE010000332         1723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_332203, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000332 DASTWE010000000
VERSION     DASTWE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332203"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1698
                     /locus_tag="VFT30_03555"
     CDS             232..1698
                     /locus_tag="VFT30_03555"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEU5091731.1"
                     /translation="MGLVLRDRLTRRAPGPTSTGECWVDREAAGCEFRDARLGKRFRQ
                     LLAQIGSAMGQSIPLICQDWANTKAAYRFFSNDRVSEADILAGHFQSTRDRVAATDGL
                     VLVLHDTTEFTYQREGSEAIGITKNINSGRDKTGRLRSHTVCGILMHSSLAVTTEGLP
                     LGLTAVKFWTRKKFKGTAALKKKINPTRVPIEKKESIRWLDNLKQSTELLNDPGRCVH
                     IGDRESDIYELFCTAQQIGTHFLVRTCVDRLAGDGNHTIAEEMDEVAVKGLHRVEVRD
                     SNGNPDEAVLEIRYRKLRVLPPIGKQKRYPALTLTVIHAEERGTPKTRKKIEWKLITD
                     LPVQSRQDAIEKLEWYALRWKIELFHKILKSGCKAEESKLRTAQRLANLISIFCILSW
                     RVFWMTMLNRSAPDAPPNLALTDVEIALLDHLVKDKGQKSFQRKPLSHYLNKIARLGG
                     YLARANDFPPGNTVMWRGLSRLTDIGLGAIVGAEIVGN"
BASE COUNT          408 a          494 c          496 g          325 t
ORIGIN      
        1 cgccgaccaa accgcgtggc tcatcgccga agtcgaagcc ctcgccaaga aatggtcccg
       61 caagcccgca agcaaacgcg cgcgacgcgc ctaacccgcg gccttcaccg gctttcgatt
      121 acccacaaat ccgacccctt ggaggggaca acgaccactg cggaatgttg agtcaaaaga
      181 atcgcttgtg attcattgct gagcacctga ttcgcgatcg aggggtgctc catgggtttg
      241 gtgttgcggg atcgactgac gaggcgcgct cccggtccta catccactgg cgaatgttgg
      301 gttgatcgag aagccgccgg gtgcgaattt cgcgacgcgc ggcttggcaa gaggttccgc
      361 caactgttgg cccagatcgg gagcgccatg gggcagagca ttcctctgat ttgccaagat
      421 tgggccaaca ccaaggctgc gtatcgcttt ttctccaatg accgggtcag cgaggcagac
      481 attctggccg gtcattttca atcgacccgt gatcgtgtcg ctgccaccga tggtctcgtt
      541 ctggtcctgc atgacacgac cgaattcaca taccaaagag agggctccga ggcgatcggc
      601 ataaccaaga acataaacag cggaagggac aagacgggcc gcctcagatc gcacacggtt
      661 tgcggcatcc tgatgcattc gagcctcgcg gtaacaaccg aggggctgcc gctcgggctg
      721 acggccgtca aattctggac ccggaagaaa ttcaagggga ccgccgcgct caagaaaaag
      781 atcaacccga cgcgagttcc catcgagaag aaggaaagca tccggtggct ggacaatctg
      841 aagcaatcga ctgagctttt gaacgatcca ggacgatgcg tccatatcgg tgatcgcgaa
      901 agcgacatct acgaactttt ctgtacggcg cagcagatcg gaacgcactt cctggtcagg
      961 acctgtgtcg accgcttggc tggggacggg aaccacacga ttgccgagga aatggatgag
     1021 gtcgccgtca agggactgca tcgcgtcgaa gtccgagaca gcaatggcaa tcccgacgag
     1081 gcggttctcg agatcaggta ccgcaagctt cgcgtcctgc cgccgatcgg caaacaaaag
     1141 cgatatcccg cgctgaccct aacggtgatc catgccgagg aacgcgggac accgaagacc
     1201 aggaaaaaga ttgagtggaa gctgatcacc gatctgcccg ttcagtctcg ccaggacgcc
     1261 atcgagaagc tcgaatggta tgcgttgagg tggaaaatcg agctgttcca caagatcctc
     1321 aaatcgggct gcaaagctga ggaatcgaaa ctcaggaccg cccaacgcct tgccaatctg
     1381 atctcgatct tttgcatcct gagttggcgg gtcttctgga tgacgatgct caaccgttca
     1441 gctccagatg cgccaccaaa tctcgcgctc accgatgtcg agatcgctct gcttgatcac
     1501 ctcgtaaagg ataaaggcca gaagtcgttc caacggaagc cactctctca ttacctgaac
     1561 aagatcgcac ggctcggcgg ctacctggca cgcgccaatg attttccccc aggcaatacg
     1621 gtcatgtggc gcggactgtc acgcctcacg gacatcgggt tgggtgccat cgtgggggca
     1681 gaaattgtgg gtaattgaaa gcttcaccgg atgcgtacgg aat
//
LOCUS       DASTWE010000333         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1107849, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000333 DASTWE010000000
VERSION     DASTWE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107849"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..878
                     /locus_tag="VFT30_03560"
     CDS             132..878
                     /locus_tag="VFT30_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091732.1"
                     /translation="MAVKSRHFIQMMVSACFAAAIFTIAACSGGYYTANMNGEKKVYR
                     VDEHGAKTLVYETDQKGKITIHDADDPMAKRQAAAQEIAKQIGSKQANRLEQLGRMTK
                     RQPTDPIYVSLAPPVLDAQMEKAERSKGAVAEQIRSEFETDPIIRLIEENQNRSGLLK
                     PHVAASAVIEVLPKVSLREAYGIDRKTGKPGKMLAVVFEATITTQVPPATYNVSESGH
                     VLRNLEVSKRFAKQVKQVILEKIGPNIPAH"
     gene            complement(1056..>1317)
                     /locus_tag="VFT30_03565"
     CDS             complement(1056..>1317)
                     /locus_tag="VFT30_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5091733.1"
                     /translation="TPNVIWYLRGGPRRFSELLADIPGVSPKVFSARLKRLERDGILT
                     RHVMPTSPPTVEYSLTNLGHELTPAIDAIVQVGHKLKRKSMP"
BASE COUNT          342 a          341 c          350 g          284 t
ORIGIN      
        1 tttcttgagt ccgtttgatg acgatcaccc aatggcttag ccggtggttt tggtgctgct
       61 acacttacct catgcatatg actttcatgt gctgtcttca ccgtacgaag agaggaaaag
      121 gagtacacct catggccgtc aaatcccgtc actttataca gatgatggtt tctgcctgtt
      181 tcgccgctgc cattttcacg attgcggcct gttccggcgg ttactatacg gccaatatga
      241 acggcgagaa gaaggtctat cgcgttgatg aacatggcgc caagacactc gtgtatgaaa
      301 ccgaccagaa aggcaaaatc accatccacg atgccgacga tccaatggct aagcggcagg
      361 ctgccgcaca agaaatcgca aaacagatcg gctcgaagca ggcgaaccga cttgaacaac
      421 taggtcggat gacgaaacgg cagccgacgg atcccattta tgtcagcctc gccccaccgg
      481 tactggatgc acaaatggag aaggccgaac gatcaaaagg ggcggtagcg gaacagattc
      541 gaagtgaatt cgaaacagat cccatcatca ggcttatcga ggaaaatcag aatcgatccg
      601 gtttgttaaa gccacatgtc gcggcgagtg cagttatcga agtgttgccg aaagtctcgc
      661 tgagggaagc ctatgggatc gaccggaaga ccggaaaacc gggcaagatg ctggcagtcg
      721 tctttgaggc cacgatcacc acgcaagtcc caccggccac gtacaatgtc tcagagtcag
      781 gccatgtctt gcgaaacttg gaagtctcca aacgtttcgc gaagcaggtt aaacaagtca
      841 tcctcgagaa aatcggcccg aatattccgg ctcactgaac cggcgactcc gcgcggcaca
      901 aaacagtctc tgccattaca gaatcgtttg gctcgcaggc gttgaatact cctcagtcca
      961 tcctgcctta ctacagtttc agagatcata agaagcgaac ggccacagtg gtatggacga
     1021 actcgtggca ggtcgagaag cgaggtttgt aatcattacg gcatcgattt ccgtttgagt
     1081 ttgtgcccga cttgcacgat ggcatcaatg gcgggtgtga gttcgtggcc caagttggtc
     1141 aatgaatatt cgacggtcgg cggagacgtg ggcatgacgt gacgcgtcaa aataccgtcc
     1201 cgttccagtc gtttgagccg tgctgaaaag acttttggcg acacgcccgg aatatcagcc
     1261 agtaattcgc tgaaccgacg aggccctcct cgcagatacc agatcacatt aggtgtc
//
LOCUS       DASTWE010000334         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_138139, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000334 DASTWE010000000
VERSION     DASTWE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138139"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..963
                     /locus_tag="VFT30_03570"
     CDS             235..963
                     /locus_tag="VFT30_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU5091734.1"
                     /translation="MLTTLGLVQSRDAAVRMILAGKVKSNGMIVDKPSRVIPIDAAIE
                     IVGESKPFVSRGGEKLEAALGSGSIDPQGLICLDVGCSTGGFTDCLLQRGAARVYAVD
                     VGYGQFDWRLRQDPRVVLIERTNIRYMPPSSVPEPIDLAVIDVSFISLTKVLPPITQF
                     LRSPALVVALIKPQFEVGKGQVGRGGVVRDDTQRAEAAQRVIRFATEMGLRTMGTVAS
                     PIKGKKGNQEILAIFAYSTPFHGM"
     gene            1003..>1707
                     /locus_tag="VFT30_03575"
     CDS             1003..>1707
                     /locus_tag="VFT30_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5091735.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLVEEGHQVVIVDNLATGKRKNVNRA
                     ASLYKTDVTSWRLERVFRNERPNIVLHLAAQVSVRRSVDDPVFDAQVNVLGTMNVLQQ
                     AVRYGCRKVVFSSSGGAIYGEQETFPAAESHATNPLSPYGISKLCGEHYLSYFQRTSG
                     IPVVSLRYANVYGPRQDPEGEAGVIAIFIQKMLNNEQPIINGNGRQTRDFVFVDDVAE
                     ANLAAMGQNAHGAYNVG"
BASE COUNT          404 a          433 c          491 g          379 t
ORIGIN      
        1 tgtcgaagaa ttgcttgaga gtgctggaag aggctgaacg gaaagtcgaa gtactggttc
       61 aggacaagaa tgggaaaaaa cagttacgtg ccttcacctc caatgacatt gaagtaccaa
      121 gttccgcaga agactcataa caggtcgttt ttctgatatc gttcttttct catcttcctc
      181 ttttcgattc tcatgagtac gaacatacga cgcgataggg atcgttgcga ccatgtgctg
      241 acgaccctgg gattggtgca gagccgagac gctgccgtcc gaatgattct tgcgggtaag
      301 gtcaagagca acggcatgat cgtcgacaag ccgtccaggg tgattcccat cgatgcagcc
      361 atcgagattg tcggagagag caagccgttc gtcagccgag gcggcgaaaa actggaagcg
      421 gccttgggct ccggttcgat tgaccctcaa gggctgatct gtctcgatgt cgggtgctcg
      481 accggagggt tcaccgactg tttgctccaa aggggagctg cgagggtcta cgcggttgat
      541 gtgggttatg gtcagttcga ttggcggctc cggcaagacc cacgcgtcgt gctgatcgag
      601 cggaccaaca tccgctatat gcctccttcc tccgtcccgg agcctattga tctggccgtc
      661 atcgatgtct catttatctc gttgacgaag gtgcttccgc ccatcacgca attcctccgc
      721 tctcctgccc tcgtcgtcgc cttgatcaaa ccacagtttg aagtgggaaa ggggcaagta
      781 ggtcgggggg gcgttgtgcg tgacgacaca cagagagcgg aggctgctca gagagtcata
      841 cggtttgcaa cggaaatggg gctacggacc atgggaaccg ttgcttcccc gatcaaaggg
      901 aaaaagggca atcaggaaat attagcaatc tttgcgtata gcaccccgtt tcatggtatg
      961 taaagagtgt cagaactgct gagagttcat tcaggagggc caatgaaagt actcgtgacg
     1021 ggcggcgcag gatttatcgg gtcgcatgtg gtcgaccgac tcgttgaaga agggcatcaa
     1081 gtcgtcatcg tcgacaatct agccactggc aagcggaaga acgtcaaccg agcggcaagt
     1141 ctctacaaaa ccgacgttac cagctggcgg cttgaacggg tctttcgcaa tgaacggccc
     1201 aacatcgtcc tccatcttgc ggctcaggtc agtgtccgcc gttcggtaga cgacccggtg
     1261 ttcgatgcgc aggtcaacgt cttgggaacc atgaacgtgt tacaacaagc ggttcggtat
     1321 ggttgtcgaa aggtcgtatt ctcctcatca ggaggagcga tctacggtga acaggaaacg
     1381 tttccggccg ctgaatctca cgccaccaac ccactatcac cctatggcat cagtaaactg
     1441 tgcggagaac attatttgtc ctacttccag cggacaagtg ggattccggt cgttagcctc
     1501 cggtacgcta acgtgtacgg gccgagacag gatcccgagg gagaagcagg ggtcattgcc
     1561 atcttcatcc aaaagatgtt gaacaacgag cagcctatta ttaatggaaa tggccgtcaa
     1621 acgcgcgatt tcgtattcgt cgacgacgtg gcagaagcta acctggccgc catgggacag
     1681 aatgcgcacg gagcttacaa cgtcgga
//
LOCUS       DASTWE010000335         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_73573, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000335 DASTWE010000000
VERSION     DASTWE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73573"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1503
                     /locus_tag="VFT30_03580"
     CDS             <1..>1503
                     /locus_tag="VFT30_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5091736.1"
                     /translation="RAGVVESVDATRIVVRADSKDGKRGKDSGLDVYDLIKFQRSNQN
                     TCITQTPVVRIGQPVKKGQVLGDGPAIDHGELALGKNVLVAFMPWGGYNFEDAILLSE
                     KLVREDAFTSIHIEEFEVEARDTKLGKEDVTRDIPNVGEEALRNLDESGIIRIGAEVK
                     PGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVKDTSLTVPPGVEGIVVDVKIFSR
                     KGLDKDERSKSIETDDQMKLQRDHHEELRIIDEEKTKKIRKLLLGKVVGRDLMDPESG
                     DVILKKKGKLTAEILRRLPDDTVRHIILSDPDEQKELEDVERRAKEQIEILQTLYDEK
                     VGRLKRGDELPPGVIKLVKVYIAMKRKIQVGDKMAGRHGNKGVVSRVLPEEDMPYLPD
                     GTPVEIVLNPLGVPSRMNVGQILETHLGWAAKALGIQVASPVFDGAAEKEIKDLLKKA
                     KLPVSGQSQLIDGKTGEPFGSPVTVGYMYVLKLHHLVDDKIHARSIGPYS"
BASE COUNT          394 a          351 c          468 g          290 t
ORIGIN      
        1 gtcgagccgg ggtcgtggaa agcgtcgatg ccacccgcat cgtggtgcgg gcagattcaa
       61 aggacggcaa aagagggaag gactccgggc tggacgtcta tgatctgatc aaattccaac
      121 ggtcgaatca aaatacatgc attactcaga ccccagttgt tcggatcggc cagccggtca
      181 agaaaggaca ggtacttggg gatggaccgg cgatcgatca tggagaactc gcgctgggca
      241 agaacgtcct cgtggcgttc atgccgtggg gtggatacaa cttcgaggac gccatattgc
      301 ttagcgaaaa attggtccgc gaggacgcct tcacctcgat tcacatcgaa gagtttgagg
      361 tggaggctcg ggataccaag ctggggaagg aagatgtgac gcgagatatt cccaatgtcg
      421 gagaagaggc gctgagaaat ttggacgaga gcgggatcat ccgcatcggc gccgaagtaa
      481 agccgggcga tattttggtg ggaaaggtga cgcccaaagg cgaaacccag ctgacccccg
      541 aagaaaagct gctccgcgcg atcttcggtg agaaggccgg cgatgtgaag gacacatcgt
      601 taactgtgcc tccgggtgtg gaaggtatcg tggtcgatgt caaaatcttc tctcgcaaag
      661 gactcgacaa agacgagcgt tcgaagagca tcgagaccga cgaccagatg aagttgcagc
      721 gagaccacca cgaagagctg cggatcatcg acgaagaaaa aacgaagaaa attcggaagc
      781 tcttgctcgg caaggtggtc ggtcgcgatc tgatggaccc cgaaagcggc gacgtcatct
      841 tgaagaagaa gggtaagctg accgcggaga ttctcaggcg attgccggac gacacggtgc
      901 gtcacatcat cctgagcgat cccgatgaac aaaaagaact ggaagatgtc gagcgacggg
      961 cgaaagagca gattgaaatc cttcagacgt tgtacgacga aaaggttgga cgcttaaagc
     1021 gaggggatga gctgcctcct ggtgtcatca agcttgtcaa ggtctatatc gcgatgaagc
     1081 gcaagattca ggtcggagac aagatggccg gtcgacacgg caataagggt gtcgtatcgc
     1141 gggtcctacc cgaagaagat atgccctatt tgccggatgg gaccccggtg gaaatcgtgc
     1201 tgaatcctct gggtgtgccg tcccgtatga acgtcgggca aattctagag acccatcttg
     1261 gatgggcggc taaggcttta ggtatccaag tggctagtcc tgtattcgat ggtgctgcgg
     1321 agaaggaaat taaggatctg ctgaaaaagg ccaaactgcc ggtgagtggc cagtcgcaac
     1381 tcatcgatgg caaaacaggc gagccattcg gcagtccggt tactgtcggg tatatgtatg
     1441 tgcttaaact ccaccacctg gtggacgata agatccacgc acggtctatc ggtccttatt
     1501 ctc
//
LOCUS       DASTWE010000336         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_267342, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000336 DASTWE010000000
VERSION     DASTWE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267342"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VFT30_03585"
     CDS             complement(<1..934)
                     /locus_tag="VFT30_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5091737.1"
                     /translation="MIEGSTVKEFAEKLGIKPKDVVTLLLQRGVFATINQPLMDDVAS
                     DLGKRFGYDVSFVPFEEMVAEEEFEELIATDADDVELPRAPVVTVMGHVDHGKTSLLD
                     GIRATDVAAGEAGGITQHIGAYSVQVPNPDSPTETRRVVFLDTPGHEAFTMMRARGAK
                     ATDIVVLVVAADDGVMPQTIEAIEHSRAANVPIIVAINKIDKPDANPDRVRQELSQQG
                     LSPLEWGGQTEMVQVSAKKRENLDTLLETILLTSDILNLRASPTRLASGVVLEAKLDR
                     GRGAVATVLVQQGTLKIHEPFIVGQISGKVRAMFN"
BASE COUNT          232 a          317 c          296 g          270 t
ORIGIN      
        1 cgttgaacat tgcccggacc ttgccggaaa tctggccaac aatgaatggc tcgtgaatct
       61 tcagtgtgcc ttgttggacc aacactgtcg caactgcacc tcgtccacga tcaagcttag
      121 cttcaagaac gacgcccgac gccaaacgag ttggactagc gcgcaggttc aaaatatcgg
      181 aagtgagcaa gattgtttca aggagtgtgt cgaggttctc gcgtttctta gccgacacct
      241 ggaccatttc cgtctgacct ccccactcga gaggcgatag tccctgttgt gagagttcct
      301 ggcgcactcg atcagggttg gcgtccggtt tatcgatttt attgatcgcc acgatgatgg
      361 ggacgttcgc cgccctggag tgttcgatcg cttcgatggt ttgaggcata actccgtcat
      421 cagcagcaac tacgaggacc acgatgtcgg ttgctttggc gccacgggca cgcatcatcg
      481 tgaatgcttc gtgacccggg gtgtcgagaa acactacacg gcgtgtttct gtcgggctgt
      541 ccgggttcgg aacttgcacg ctgtaagcgc cgatgtgctg cgtaatgcca cctgcttcac
      601 cagctgcaac atccgtagct cggatgccgt caagtaagga tgtcttaccg tggtccacgt
      661 ggcccatgac tgtgactacg ggtgcgcgcg gcagttccac gtcatcagca tcagtggcga
      721 taagttcctc aaactcttcc tcggccacca tctcttcaaa cggcacaaat gaaacgtcgt
      781 agccaaaacg tttgcctaaa tcactggcca cgtcgtccat cagcggttga ttgatggttg
      841 cgaagactcc ccgttgaaga agcagggtca ctacgtcctt aggtttgatg ccaagcttct
      901 ctgcaaattc cttaacggtg gacccttcaa tcaagcgaac gcttttcagc tcgccactct
      961 gtggaagctc taagcgtccc gcgatacggt cctcaagcga aagggccctt ggtggcggga
     1021 tgaaatcggt tttatcaaac ctgccagcct tgccttcaat ctttcgacct ccgcgcggac
     1081 tacttcgtct tgagcggcgt cgaccggtat ccggc
//
LOCUS       DASTWE010000337         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_493812, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000337 DASTWE010000000
VERSION     DASTWE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493812"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..1414
                     /locus_tag="VFT30_03590"
     CDS             515..1414
                     /locus_tag="VFT30_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5091738.1"
                     /translation="MACVISVASGKGGVGKSVVSVNLALVLARMGKQVVLADLDVGGA
                     DAHVMVGLLNPLLTLTDFLNHRIEHLDDLAQRLSIDPNLRIIPGTGDTLATANMTYAQ
                     KKRLIRNFQNIRADVIIVDIGAGASYHTLDFFLMADHHITVATPDPTSVLDLYRFIKL
                     AAIRRVLSMFMMRDVMAEGLANRDYSSVEEVLDVAGKTDESGRAFAETTLRAFQPTLV
                     LNRVSGRARVNVLQLRKLLKEYVGGDLTLLGEIPEDPAMEQAVRAYVPVVQHAPSSPA
                     AVALAKTADALAQLLRSASPQAA"
     gene            complement(1532..>1958)
                     /locus_tag="VFT30_03595"
     CDS             complement(1532..>1958)
                     /locus_tag="VFT30_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091739.1"
                     /translation="TTNGDQYVVDDICHDPLHGDKLVAVYAAAVRKEGKTNGKIIGVL
                     GVVFDWEEQSSTIVQKEPAISEASWKRTRVMLLDNRLRVIAASDHQGVLMPFILQHQG
                     RQRGHYVNDKHEVIAYAKTLGYQEYDGLGWYAVIVQRLH"
BASE COUNT          420 a          560 c          501 g          477 t
ORIGIN      
        1 tgcccccgga agaacgtcgc cggtaggttg atgccgccac tcatgacctc ggctccttgc
       61 cggctgagtc attctgccga tgaaccggac agcagctcac aggtctctag ccttgccgag
      121 accgaccatg catccattca tggatatcac ggcgaaggaa gcgccaatgt tttccaattt
      181 ttgacgctgg cagttcacca actcgggcaa gcttatagac ggtggacttc ggtacccgga
      241 ggaaccgagc cacctccatc acggtcagaa tttccctctc ccgtgcggtg gcctcctcgg
      301 cctggatcgc tgttgctgta gatccttcat tattcatgac gactgaggta tcggcgtggc
      361 tgttccaaaa cttgagcacc cgataggaat ggatctcggt ttacacctct attttcttac
      421 ccattcctct cggatggatg aggtcattca tatttctccg cgactgaccg acaaaggatg
      481 tggactggct ccaaaccagt aggaactcaa ctcaatggcg tgtgtgatct cggttgcatc
      541 aggaaaaggt ggagtgggga aaagtgtagt ttcggtcaac ctggcgctcg tcttggcacg
      601 gatgggaaag caggttgtgc tcgcagacct cgacgtcggc ggcgccgacg cgcatgtgat
      661 ggtcggcctc cttaacccgc tgctcaccct gactgacttc ctcaaccatc gaatcgaaca
      721 tctggacgat ctggctcaac gtctttctat tgacccgaac ttgcggatca ttcccggcac
      781 cggcgatacg ttggcgacgg ccaacatgac ttatgcacag aaaaagcgcc tgatccggaa
      841 ttttcagaat atccgagcgg acgtgatcat cgtcgatatc ggagcaggag cgagctatca
      901 tacactcgat ttttttctga tggccgacca tcatattacg gtcgcaacac cggacccgac
      961 ctccgtcctg gatctctacc ggttcatcaa actagcggcg attcgtcgcg tcttatcgat
     1021 gttcatgatg cgggatgtca tggcggaggg gcttgcgaat cgggattaca gcagtgtgga
     1081 agaggtgttg gacgttgcgg gcaagaccga cgaatcgggt cgggcatttg cagaaaccac
     1141 gctgcgagct tttcagccaa ccctcgttct caatcgcgtg tcgggacgtg cccgcgtcaa
     1201 tgtcctgcag ctcaggaagt tgctcaagga gtatgttggc ggtgacctaa ccttattggg
     1261 tgaaattccc gaagaccctg cgatggagca agcggtacgc gcctacgtcc ctgtagttca
     1321 gcatgctcca tcctctcctg ccgctgtcgc gcttgcgaaa accgccgatg ccctggcaca
     1381 actgcttcgc agcgcatcgc cgcaagcagc ttgataggcg agacccaaat attctccacg
     1441 ccgtttctca tcaatcgttg ctcgttactc tcaaaggaac cgtacttcga acccgggcga
     1501 acctggcgct tgctcccttg aactctactg atcaatgaag cctttgcacg atcaccgcat
     1561 accatccgag gccatcgtat tcctggtagc cgagagtttt cgcatacgcg atcacttcgt
     1621 gtttgtcgtt cacgtaatgg cctcgttggc gtccttgatg ttgaagtatg aacggcataa
     1681 gtactccttg atgatcggat gccgcaataa cacgcagcct gttatccagc agcatcacgc
     1741 gagtgcgttt ccacgaagct tcggatattg ccggttcttt ctgaacaata gtgctggatt
     1801 gttcttccca atcaaatacc acccctaaaa cgccgatgat ctttccattc gttttcccct
     1861 ctttccgaac agcggcggcg tacacggcga ccaatttatc gccatggagt ggatcgtgac
     1921 aaatatcatc gactacatac tggtcaccgt tcgtcgtc
//
LOCUS       DASTWE010000338         1198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_170662, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000338 DASTWE010000000
VERSION     DASTWE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170662"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1043
                     /locus_tag="VFT30_03600"
     CDS             321..1043
                     /locus_tag="VFT30_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091740.1"
                     /translation="MHKETIVDPWSLSIWAVLVVVVTAGCVPDDNSINSSRVENETLK
                     KQVARQEALLSSLLDGNKVMQQQIDLLNQELRDAKSTTEATKAEAKHQTEQLELQLAE
                     TKRMIATQMTESLKIEEKGAQSDTLPRPMPAVAKVVEDALARNGYPLKVSVKTDQRAV
                     YVTERKISNPTSLEVTGSRNQYLIFLQALPDNLTKLGVKAEFERVAQGGRILSVSHEE
                     TNEIEHRLINEINKALANPAKT"
BASE COUNT          313 a          343 c          318 g          224 t
ORIGIN      
        1 caccgccggg gtcgatgacg accgcctgct tcgtgagagg gtcgccgatg atcgagcagt
       61 tacagccaag aggtggaacc gagaaggtct tccgaatcaa agcagtcatg atatgagcat
      121 gctacagtcc aacgagctag aagcgcaagc taacgccgca cctcatcaga tctcagaagc
      181 gaggagtctg tttttttacg attcggtcgt accggcccta gtctccaact gaaaggtgaa
      241 gcaagatacg gccgaatacc acgtggacaa gttgagctga accgtagata atctcacgac
      301 tctactggga gggctgtcgc gtgcacaagg aaacgatcgt tgatccatgg tcactaagca
      361 tctgggccgt attggtggtg gtggttaccg ccggctgcgt tcctgacgac aattccatta
      421 attccagtcg cgtggagaat gagaccctca agaagcaagt cgcaaggcaa gaagccctcc
      481 taagctcctt actggacggg aataaggtta tgcagcagca gatcgatctg ctcaatcaag
      541 aactccggga tgcaaagagc accacggaag ccacgaaggc ggaagccaaa catcaaaccg
      601 agcagctgga actgcagctg gctgagacca aacgaatgat tgctacgcag atgaccgaat
      661 ccctcaaaat cgaggagaaa ggcgcccaat ccgataccct cccccggcct atgccggccg
      721 ttgcaaaggt cgttgaggac gcactggcac gcaacggtta tccgctgaag gtcagtgtca
      781 agaccgatca acgggcggtc tatgtgacgg agcgcaagat ctccaacccc acgtcactcg
      841 aagtgacggg ctcgcggaat caatacctca ttttccttca agccctcccc gacaatctga
      901 cgaaactcgg tgtgaaagcg gaattcgaac gggtcgcaca ggggggccgc atcttaagcg
      961 tcagccacga agaaacgaac gaaatcgagc atcgcctcat caacgaaatc aacaaggcgc
     1021 tcgccaatcc agccaagaca taactcttcg gcatgcggcg gtcacaatca ccggttttct
     1081 tggtccggtc gaggcctcgt gctagcattt ccctccgatg gcactctcga ccagcgtgca
     1141 cgatcttcgc accctgatcc gctcctccca tcctctcgtc gtcattgaaa cggtggag
//
LOCUS       DASTWE010000339         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1269351, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000339 DASTWE010000000
VERSION     DASTWE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269351"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..2287)
                     /locus_tag="VFT30_03605"
     CDS             complement(77..2287)
                     /locus_tag="VFT30_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU5091741.1"
                     /translation="MDIRKRFLRSCFIGLLSTSPIHALGVSALFAQENVAGIHHTLSA
                     ELIPATHELAATDQIELETEPQVTSVTFTLAHTLHVESVTMRTHSVSGEQGRSGEVVP
                     FTKVRPAETTVQRIAVSLPKEHGRRVTLVMRYHGQINDPPREPRHLRFVTPSETAGHI
                     GLEGVYLSGESQWYPDVSGSFSTYRVTAKIPQGWTVVASGRKEGETTSAGKTASTWIV
                     QDQSEAFTLVANKFVTTSREWKGPTGQRVKLQTYFLPDNSGLADEYLDATARYLDAYV
                     RILGSYPFDKFAVVENFFASGLGLPSFTLLGSGSIKRHYVQPYALGHEIVHSWIGNSV
                     FNRDDHGNWVEGLTTYLANYYWHELVQDVPQAFEQRRMMLDGYNLYVRPETDYAVSQF
                     LRKHDERDNAIGYQKSAFVFHLLRQEVGDEPFWRGLKTFVDSYRSRPADWRSIEEVFS
                     RESGQDLRWFFEQWVEQPGAPRVSLRDVHAHRVKEEGGKETWRLTVQVDQAGNPFRMG
                     IPIRIVMKESTEIRTIALSLSQMSVTEFILPNQPLRVELDPNLMTLRRLTRRQLPPML
                     NGYVTDQPKTVVLAFSDPASPLRQIVSRIADHELTGSFRTTVVSLKENSLPRDGSILV
                     LAGAEERQAVQSVVQESCGDRVALGERNFQIDGRTYNGPTMAVLFSCHRTNVPGSVIT
                     VLYGVTSGAVEKLSRFLFYYGWHSYVIFQDGAVTKREVWQSSPDMKEVRIDTTS"
     gene            complement(2299..3084)
                     /locus_tag="VFT30_03610"
     CDS             complement(2299..3084)
                     /locus_tag="VFT30_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091742.1"
                     /translation="MIKVLIVDDDQMNCDLLQSVFTRQGYHVISTTSGREGLDLFRVH
                     NPRVTLLDLRMPEMDGLTVLKEIRAIDPHAPVIILGGGATEIQENQARALRATDFIRK
                     GLSLDVLVEAVNRLSKLPVSSMTTQLPLCNGNVIEQIDESVLVVDDEPLLCDLLVRFL
                     SLRGYRAFGVRDGHEALRVAEDMSPDAIVLDLIMPGMPGIEVLRTLRDKGYPGGIIIM
                     TGSHNEELLREAWSMGPQEILIKPVDLELLLTAIQLVLVCREC"
     gene            complement(3172..>3980)
                     /gene="ftsY"
                     /locus_tag="VFT30_03615"
     CDS             complement(3172..>3980)
                     /gene="ftsY"
                     /locus_tag="VFT30_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5091743.1"
                     /translation="AADLGASVVDRMMRRVREETRGVEANTSEGIQNVLSRSLYSILK
                     TVSSPTIDQLVDRSSKPFVTLVVGINGVGKTTTIAKIAQRMTQAGRRLLLVAGDTFRA
                     AAIEQLQVWGDRVGVEVIRQRHGADPAAVAFDGIVAAKARMVDMVLVDTAGRLHTKSN
                     LMDELRKVKRVIAQELPGAPHEVLLVLDATVGQNALAQARQFHEAVGVTGLALTKLDG
                     TARGGIVVAIAEELKLPLRLIGVGEGPDDLQDFNAEAFVAALFGQPTSRS"
BASE COUNT          929 a         1141 c         1003 g          907 t
ORIGIN      
        1 cggctgatcc gcagctagtg caaccggggg aatactcatt aatgcacaga acccccatag
       61 ccaaattcct ccgatcttat gacgtggtat caatcctgac ctccttcata tccggcgaac
      121 tttgccacac ttcgcgtttt gtcacggctc catcttgaaa aataacgtaa ctatgccacc
      181 cataataaaa caggaagcgc gaaagttttt cgactgcgcc ggaggtcacg ccatacagca
      241 ccgtgatgac gctgcctggc acattcgtcc gatggcagga gaagagcact gccatcgttg
      301 gtccattata cgtccgacca tcaatctgga aattcctctc tccgagagca acccgatcac
      361 cgcatgattc ctgcaccact gattgaaccg cttgtcgctc ttcggctcct gccaaaacca
      421 ggattgatcc atcgcggggt agggaatttt cttttagaga gaccaccgtc gtcctaaacg
      481 atcccgtcag ttcgtgatcg gcgatgcgag acacaatctg ccgcaacggc gaagcagggt
      541 cagaaaacgc caggaccacc gtctttggtt gatccgtcac atagccattc agcatgggcg
      601 gcaactgacg tctcgtcagg cggcgcaaag tcatgaggtt gggatcaagc tccacccgca
      661 gcggctgatt gggaaggatg aactccgtta cgctcatttg cgaaaggcta agcgcgatcg
      721 tcctgatttc cgttgattcc ttcatcacga tgcggatagg gatccccatc cgaaacggat
      781 ttccggcctg gtcaacctga accgtcagcc gccaagtttc tttgccgccc tcttccttca
      841 cgcgatgagc gtggacatcg cgcaaggata ctcgtggagc accgggctgt tcgacccatt
      901 gctcgaaaaa ccaccgcaag tcctgaccgc tttctcgaga aaagacctcc tcgatcgatc
      961 tccagtctgc cgggcgactg cgataactgt cgacgaaggt cttcaaaccg cgccagaatg
     1021 gctcgtctcc gacttcttgt cgaaggagat gaaacacaaa ggctgatttt tggtagccga
     1081 tagcattatc tctctcgtca tgtttccgaa gaaattgtga gaccgcatag tctgtttcag
     1141 gcctcacgta gagattatat ccgtccagca tcattcgtct ttgttcgaag gcctggggaa
     1201 catcctgaac caactcgtgc cagtagtaat tcgccaagta ggtcgtcagt ccctcgaccc
     1261 agttgccatg atcgtctcga ttgaagaccg aattcccaat ccatgaatgg acgatctcgt
     1321 gacccaaggc atagggttgg acatagtgtc gcttgatgct accgctgccg agtaaggtga
     1381 acgatggcag accgagaccg ctcgcaaaaa aattctcgac caccgcaaat ttgtcgaaag
     1441 gatagctccc caggattctg acatatgcat cgagatacct tgcagttgca tcgagatact
     1501 catccgccaa gccggaattg tcgggcaaga aataggtctg gagtttgact cgctggcctg
     1561 ttggaccttt ccattcccgc gacgtcgtca caaatttatt ggcaaccaat gtgaacgcct
     1621 cagactggtc ctgaacgatc caggttgagg ctgtctttcc cgcgctcgtc gtctcgcctt
     1681 ccttacgccc ggatgccact accgtccagc cctgtggaat cttggcagtt acccgatagg
     1741 tactgaagga gccgctgaca tcggggtacc attggctttc tccgctcaag tacacacctt
     1801 ccaaaccgat atgtcccgca gtctcactgg gtgtcacgaa tctcaaatgg cgcggctccc
     1861 tcggagggtc attgatttgg ccgtgatacc tcatgaccaa tgtcacccta cgtccatgct
     1921 cttttggaag ggagaccgca atccgctgaa ccgtggtttc agcagggcgc actttcgtga
     1981 acgggacaac ttcgcccgat cttccctgct cacccgagac cgagtgcgtt cgcatagtga
     2041 cggactcgac atgcagtgta tgagcaagcg taaacgtgac tgacgtgacc tgtggctcgg
     2101 tctctagttc gatctgatcg gtcgccgcta gctcgtgtgt cgcgggaatc aactctgcgg
     2161 agagggtatg atgaataccg gcgacattct cttgggcaaa caaggccgaa acgcccaacg
     2221 cgtgtatggg agaggtcgac agcaggccga tgaagcatga gcgaagaaaa cgcttcctga
     2281 tgtccatcag gaagcgtttt agcactcgcg gcagacgagt acaagctgga tcgcggtcaa
     2341 gaggagttca agatcgaccg gttttatgag gatctcctga gggcccatgc tccaagcttc
     2401 tcgaagcaac tcctcattgt gacttccagt catgatgata attccacctg gatagccctt
     2461 atcacgcagg gtgcgaagca cttcgatccc tggcattccg ggcatgatca ggtcgagtac
     2521 gatcgcatcg ggcgacatat cttcagctac ccgcagagct tcgtggccat ctctgacgcc
     2581 aaacgcccga taaccacgca gactgagaaa ccgcaccaag aggtcgcaca ggagaggctc
     2641 gtcatcgact accaagaccg attcatcgat ctgctcgatg acattcccat tgcaaagagg
     2701 gagctgcgta gtcatcgacg acaccggcag ttttgagagt cgattgacgg cttcgaccag
     2761 gacatccagt gacaaccctt ttctgataaa atccgtggct cgtagggctc gtgcttgatt
     2821 ctcctgaatt tcggttgctc caccgcccag gataatcacc ggcgcatgag ggtcgatagc
     2881 ccggatttcc tttaagacag tcaatccgtc catctccggc atacgaaggt ccagcagggt
     2941 aacccgtgga ttgtgtacac ggaatagatc aagaccttct cgtccactgg ttgtcgaaat
     3001 gacgtgatac ccttgccggg taaacacact ctgcaacaga tcgcaattca tctgatcgtc
     3061 atccacgatc aagactttta tcatcgctgc cggttctcct cagcctggaa agctgtacct
     3121 gtgaaaagag aatagcacga ggccttgaac aacgagaaga caatgcgtgg tttatgagcg
     3181 agaagtcggc tgcccgaaca gagccgcgac aaatgcctcg gcattgaagt cctgaaggtc
     3241 atcgggtcct tctcccacgc cgattaggcg cagtggaagc ttcagttctt cggcaatcgc
     3301 gacgacaata cctcctcgtg cagtcccatc aagcttcgtc agagcaagac cagtgacccc
     3361 aactgcctcg tgaaattgac gagcctgggc cagcgcgttc tgtccgaccg tggcatccag
     3421 gaccaacaac acttcatgtg gcgcaccggg taactcctga gcgatcacac gttttacttt
     3481 ccgcagctca tccatcagat tggactttgt gtgcaagcgg cctgccgtat caactagaac
     3541 catgtctacc attctggctt ttgctgcaac gataccgtcg aaggccacgg ccgcaggatc
     3601 ggcaccgtgt cgttggcgaa tcacctccac tccgacccga tctccccaaa cttgcagctg
     3661 ttcaatggcg gctgcacgaa aggtatctcc tgcgacaagg aggagtcgcc gcccggcttg
     3721 cgtcattcgc tgtgcaattt tggcgatggt agttgttttt cctacgccat tgattccgac
     3781 gacaagggtg acaaacggtt tagagcttct gtcgaccagc tgatcgatgg taggactcga
     3841 tacggttttt agaatactat agagcgaccg acttaagaca ttctgaatcc cttctgacgt
     3901 gttggcttcc actccgcgtg tctcttcccg gacacgccgc atcatacgat cgacgacaga
     3961 tgcgccaagg tcagcagcga
//
LOCUS       DASTWE010000340         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1366298, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000340 DASTWE010000000
VERSION     DASTWE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366298"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFT30_03620"
     CDS             complement(<1..202)
                     /locus_tag="VFT30_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5091744.1"
                     /translation="MHVLMNYCSHCGATVTHKIPLGDTLPRHVCDQCERIHYHNPKIV
                     AGCIPEWEDQVLLCRRAIEPRLG"
     gene            451..>1203
                     /locus_tag="VFT30_03625"
     CDS             451..>1203
                     /locus_tag="VFT30_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5091745.1"
                     /translation="MENSNVLSTLPILPVKRTVLFPGVMMPLTIGRERSVAAVNAAMK
                     TEEKTIVVVAQRDPQAEEPALADLYSIGTKAIVKQLGQSSEGTIHALVQGLDRVVLLK
                     EEQSTPYSIVRVRSLGRPTADGPEVQALHRAIQELVSDLPRLIQAPGIQEVGAVLSNE
                     DDSVALAYRIASLVNLDVVEEQRLLESSSTLDLLRSLYAALSKEIQILQLREKIAKDA
                     QTKIGKNQREYILREQLKAIQDELGEGEGEENE"
BASE COUNT          294 a          319 c          328 g          262 t
ORIGIN      
        1 accccaagcg aggttcgatg gcccgacggc acaagaggac ttgatcttcc cattcaggaa
       61 tacagcctgc gacgattttg ggattgtgat aatgaatgcg ctcgcactga tcgcacacgt
      121 gcctaggcag agtatccccg agaggaattt tatgcgtgac ggttgcgcca cagtggctgc
      181 aatagttcat gagaacatgc atcggagcgt tctcgttgcg aattcggaca cgtacggctc
      241 atgaaccagt actgagcatg gatagcacaa acacgctttg ctttgcacgc cgagtcgata
      301 agaacctttc aagatcgccg acatgcagca atgtgaacct cttttccttt cctcttactg
      361 ctcgtccaag atttccttcc cttgacttcc acgctgggga acctatcagg ttgttataat
      421 aacgtgaacc ctgaggaagg atggacttcc atggaaaata gtaatgtgtt gtcgacattg
      481 ccgatactcc ccgtcaaacg gacggtattg tttccaggag tgatgatgcc actgacgatc
      541 gggcgggagc ggtccgtcgc tgccgtcaac gccgcaatga agaccgaaga gaagacgatt
      601 gtcgtggtgg cacaacgaga cccgcaggct gaagaaccgg cactcgccga tttgtattct
      661 atcggcacga aggcgattgt caaacaactt gggcagtcgt ccgagggaac gattcacgcg
      721 ctggttcagg gattggatcg cgtcgttctc ctgaaagaag aacagtccac cccctactcc
      781 atcgtgcgtg tccgttcttt gggacgtcct acggccgatg gaccggaagt ccaagcgctt
      841 caccgagcca ttcaagagct tgtgtccgat ttaccgagac taatccaggc tcccggcatc
      901 caagaagtcg gcgcggtgct cagcaacgag gacgattccg tggcgcttgc ctaccgtatc
      961 gcttcgctcg tcaatcttga cgtggtggaa gaacagcgct tgctcgaaag ttcttcgact
     1021 ttggacctcc ttcgcagcct ttatgccgcg ctgtcgaagg aaattcaaat actgcagtta
     1081 cgggaaaaga tcgccaaaga tgcgcagacc aaaatcggca agaaccaacg agagtacatc
     1141 ttgcgcgagc agctgaaagc catccaggac gagcttggcg aaggcgaggg cgaggagaac
     1201 gag
//
LOCUS       DASTWE010000341         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_849214, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000341 DASTWE010000000
VERSION     DASTWE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849214"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1542)
                     /gene="rseP"
                     /locus_tag="VFT30_03630"
     CDS             complement(151..1542)
                     /gene="rseP"
                     /locus_tag="VFT30_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU5091746.1"
                     /translation="MTSVLGWSPDTLWLLLQKAWWFLVVLGVLVAFHELGHFLAARWV
                     GVKVLKFSIGFGPKLFGRQVGETEYLVSAVPLGGYVKLFGEDETEAITPDDRRRSFSH
                     QGLWGKVLIVAAGPGFNFILAYLIFAGWLSTGAPLFVPTFRDLSADVEALVPGSPASM
                     AGMEVGDRIVKVNGKDISTKTELLDIISKSKGQPVALEVRRVDQLKTLTATPILVTGD
                     RAGGDEPQYTIGVEETPPLVTSVMQGSPAALAGFKPGDRVVAIEGQVIYTWAQMTTQV
                     REHPQKSLKLEVLRDDKRTALTVTPTSEKVTVNGQTLEVGKIGISGPGRSLMRSDNAA
                     EAVYQGLEATWGWTELTAIGLYKMIVGDISSKNIGGPLTIANISGEAASQGASSVVFL
                     IAILSINLGVLNLLPIPILDGGHLLFFLIEGILRKPLGERQREVAQQVGLVLLVGVMI
                     FAFWNDLERIFSR"
     gene            complement(1616..>2285)
                     /locus_tag="VFT30_03635"
     CDS             complement(1616..>2285)
                     /locus_tag="VFT30_03635"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5091747.1"
                     /translation="DVRRLILTASGGALWTLTQEQLMNVTPERALQHPNWKMGSKITI
                     DSATLMNKGLEVVEARWLFDIPESNIDVLVHRESIIHSLVEYKDRSMIAQLGLPDMRT
                     PISYAMRHPARMALDLPSLDLTEIGQLTFCQPDHDRFPCLNLGYESLRIGGTMPAAMN
                     AANEIAVEAFLNHGLRFSEIPQVIRSTMEAHAQQEMTCLDDALEADRWAREKAESLVH
                     ALPR"
BASE COUNT          524 a          632 c          625 g          504 t
ORIGIN      
        1 ctaatgaggc cgcaggcgaa aagaaaccgg aggtgtattc gttctcaccc gcccgccccg
       61 agctgccaag acagtttttt gcctcgatgg ggtacgttga ggatttcttg gagccgagaa
      121 cgaagttagg aactggcttc agcattctgc ctaccgggag aagatgcgtt ccaagtcatt
      181 ccagaaggcg aagatcatca caccgaccaa caagaccaat cctacctgct gggcaacctc
      241 tcgttgccgt tcgccgagcg gtttgcggag aatgccctca atcagaaaaa agagcaagtg
      301 gccgccgtcg aggattggga tcggcaataa attgaggaca ccgaggttga tgctcaagat
      361 tgcgatcaga aacacgacac tggaggcacc ttgggaagca gcttcaccgg aaatattggc
      421 gatcgtcagc ggtccaccga tattcttgct cgaaatatca cccacgatca tcttatagag
      481 gccgatcgcg gtcaattcgg tccatcccca tgtggcttcc agtccttgat acacagcttc
      541 tgccgcattg tccgagcgca tcaacgaacg gccgggtccc gagatgccga ttttccccac
      601 ttcgagggtc tgtccgttca ccgttacttt ttcactggtc ggcgtgacgg tcagtgcggt
      661 acgtttgtcg tcgcggagga cctcaagttt cagcgatttc tgcgggtgct cccttacctg
      721 ggtggtcatc tgcgcccatg tatagatgac ttgcccttct atcgcgacca ctcgatcgcc
      781 cggcttgaat ccggctagag cggcgggcga tccttgcatg accgaggtga caagtggagg
      841 cgtttcctct acaccgatcg tgtactgagg ttcgtcccca ccggccctat ctccggtaac
      901 tagaatagga gtcgctgtca gtgttttcag ctgatcaact cggcggactt cgagtgccac
      961 cggctggcct ttgctcttgg agataatatc cagcaactct gttttggtcg agatgtcttt
     1021 gccgttgacc ttgacgatgc ggtcgccgac ttccatgcct gccattgatg cgggagaacc
     1081 gggaaccagg gcttcgacat cggcactcag atcacggaac gtggggacaa acaacggcgc
     1141 accagtggac aaccatccgg cgaagatcag ataggccaga ataaaattga acccagggcc
     1201 cgccgccacg ataagaacct ttccccacag gccctggtgc gaaaacgatc gccggcggtc
     1261 gtcgggtgtt attgcctccg tctcatcctc cccaaacaac ttgacgtacc cgccgagcgg
     1321 tacggcagaa acgagatact cggtttcgcc gacctgtcgg ccgaataact tcgggccgaa
     1381 gccgatggaa aacttgagga cttttactcc aacccatcga gcggcgagaa agtggccgag
     1441 ctcatggaag gcgaccagta cgccaaggac cacaagaaac caccaggctt tctgaagaag
     1501 cagccacaat gtatcaggag accagccaag tacggacgtc atcggtaact ccgtgtcgcg
     1561 tgtgtcgtgt gtgatgttct actttaacat tcgcggtgtg aaatgcaaac gatcgttagc
     1621 gaggtaacgc atgcaccaac gattcggcct tttctcgagc ccatcgatcc gcttccaacg
     1681 catcatcaag acaggtcatc tcctgttggg catgagcctc catcgtactg cgaatgacct
     1741 gagggatttc actgaagcga aggccatggt taaggaacgc ctcgacggcg atttcattcg
     1801 ccgcattcat cgcggcgggc atagtcccgc cgattcgaag cgactcgtac ccgagattga
     1861 ggcaaggaaa acgatcatgg tcgggttggc aaaaggtcaa ctgtccgatc tccgtcaaat
     1921 ccagtgaagg caggtcaagg gccattctgg cgggatgacg catcgcgtag gagatagggg
     1981 ttcgcatatc cggaagtccc agttgggcaa tcatggaccg atctttatat tctaccagag
     2041 aatgaatgat gctttcccga tgaaccaaga cgtcgatgtt ggactcagga atatcgaaga
     2101 gccagcgggc ttccacaact tctaatcctt tattcatcaa cgtggccgag tcgatggtga
     2161 tcttggatcc catcttccag ttgggatgtt gcagggctcg ttccggtgtc acattcatga
     2221 gctgttcctg ggtcagtgtc cagagggctc cgccggatgc cgtgagaatc aatcgccgga
     2281 cgtct
//
LOCUS       DASTWE010000342         1400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_9100, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000342 DASTWE010000000
VERSION     DASTWE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9100"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VFT30_03640"
     CDS             complement(<1..1374)
                     /locus_tag="VFT30_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU5091748.1"
                     /translation="MIRLTLRREPASVQQLQHEVPLFLNRLAQVFEGAGASPLPASPP
                     YCIDTVSSNSVRPDPIVSVEGPATDLSDRYLIPDLDPEVLSYFIPEAQEYLELLETNL
                     LRLDKDPQNKELINQLFRSAHTLKGSAYTVGFQSIGDLVHHVEDFMMALRDDGLRVLP
                     GHTDLILRAIDVVRVLMRRDLDKVDNARQRFETARTDLRRLDQGTASEKTPAFQPDDT
                     SGSATEERSRSDNYRGHDDRVAGEKSREERDVIRVSYARLERLMNLVGELVIGRGRLE
                     QRLRVLEQLSQQVLTFKGRLVDSVQSFADKHTFTYQETPTSSTAPAGQGLPAFGDFGN
                     LELDKYDDFNILARRIGEITADISESMSQLDGSIERTHDEMSQLQHLTLLMRDEIARA
                     RMVPIGTPFTRFRRAVREIARASNKEVSLVTSGEQTEVDTGVVERLVDPLVHLVRNAV
                     YHGIEPTA"
BASE COUNT          316 a          385 c          351 g          348 t
ORIGIN      
        1 ggccgtcggc tcgataccgt gatagacggc attcctgacc aaatgcacca atggatccac
       61 caatcgttcc acgacgccgg tatcaacttc cgtctgctcg cccgatgtga ccaaggacac
      121 ttccttgttt gaggctcgcg cgatttctct gaccgctcga cgaaatcttg tgaatggtgt
      181 cccgatcgga accatgcggg cacgagctat ttcgtctcgc atcaacaacg tcaagtgttg
      241 cagttgactc atctcatcat gggttctttc gatggatcca tctagctgcg acatcgactc
      301 actgatatca gccgtgatct ccccaatgcg ccgagctaaa atattgaaat cgtcgtattt
      361 atccagctcg agattgccaa aatcgccgaa tgcgggaagt ccttgacccg caggcgcagt
      421 cgaactagtc ggtgtttcct gataagtaaa agtgtgtttg tcggcaaagg attggacgga
      481 atccactaaa cgacccttga atgtcaatac ttgctgcgac agttgctcca ggactcgcag
      541 tcgttgttct aatcggccgc gtccgatgac aagctctcct acgaggttca tcagtcgctc
      601 aagccgcgca taactgacgc ggatgacgtc acgttcttct ctcgatttct cacccgcaac
      661 tctatcgtca tgtccacgat agttgtcgct tcgggagcgt tcttcagtcg cagaaccaga
      721 ggtgtcatcc ggttgaaatg ccggcgtctt ctcgcttgca gtaccctgat ccaatcgtct
      781 caggtctgtt cgcgcggtct cgaatcgttg cctcgcatta tccaccttgt cgaggtctct
      841 ccgcatgagt actcgtacaa catcgatggc gcgaagtatc agatccgtat gcccaggcaa
      901 cactctgaga ccatcatctc gcagtgccat catgaagtct tcgacgtgat ggactaggtc
      961 gccgatcgac tgaaatccaa ctgtgtaggc ggaacctttc aaagtgtgtg cgctcctaaa
     1021 cagctgattg atcaattcct tgttttgtgg gtctttgtcc aatcgtagga gattggtttc
     1081 caacagctcc agatactcct gcgcctcagg gataaagtag gagaggactt ccggatccaa
     1141 atccggaatc aagtaccggt cggataggtc cgtggcaggc ccttccacgc ttacaatggg
     1201 gtcaggccgg acagaattcg agctaaccgt atcgatacag taaggcggag atgccggtag
     1261 aggtgacgcc cctgcacctt caaacacctg agcgaggcga ttgagaaaaa gtggtacttc
     1321 gtgttgaagt tgctgcacac tcgctggctc acgtcggaga gtgagccgga tcaattcagc
     1381 tcctttcgtc agcatgtcaa
//
LOCUS       DASTWE010000343         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1398937, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000343 DASTWE010000000
VERSION     DASTWE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398937"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="VFT30_03645"
     CDS             complement(<1..1780)
                     /locus_tag="VFT30_03645"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEU5091749.1"
                     /translation="MPFDQALRFASIWLASMSFIGLTLGAGLPEWLAALTGAALIVAL
                     IRNRGGKSVERLATFASISAAGWNLLVIVGFLGFWVDMLWISGELLPAGIHFLMILMI
                     IKLFNLKLRRDYLHLYAISLVAILASGSLTTDLWYLPIFLLYLLAGVWTLLLFQLTKH
                     QEETRMLAMSVPMRPQPPASVNQVTPQLFWLANGLAFATFGLTLVIFFTIPRVSAGFY
                     QKGFGENIRTSGFSETVNLGEIGPIKRDPSVVMRVELSDGSPHETGPLYLRGVAFDQY
                     DGKTWTNRLNHRRSVSEDTTGTFVLRGGRSPAPARLGVTIRQNILLEPLDTPVLFAAP
                     FIERVSGKFPSVQSDLTGSVYLPFPSSSRIEYSVLSRSNPVLPADLGLEPSRYSESFI
                     RHFLQIPAQSERIAILAKEITDTQRTMYDKANAIQSYLTHNFRYSLDAPLTGQDRPLE
                     EFLFTRKTGYCEHYATAMVIMLRAIGVPARLVTGFLATEWNEYGNYYLVRQQDAHAWV
                     EVHLPHSGWVSMDPTPPSIESIDSGSPAWHALGRMMDTIRLQWSRFFVQYSAADQLAV
                     VRELKAGGTSARNKAFDSMSSLFSPFL"
     gene            complement(1770..>2029)
                     /locus_tag="VFT30_03650"
     CDS             complement(1770..>2029)
                     /locus_tag="VFT30_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091750.1"
                     /translation="MVGSSQSSFGQGDLHLLELLRMLALCERCFPLAESAVPGELSRN
                     LEHEGIAMIVVHSWLGPMDIEREHPTILINGAVITGASHAV"
BASE COUNT          497 a          550 c          582 g          400 t
ORIGIN      
        1 ccaggaaagg gctgaagagc gaactcatgg aatcgaatgc cttgttgcgg gccgacgtac
       61 cgcccgcttt caattcccgt acaacggcaa gctgatccgc cgcactgtac tgcacaaaaa
      121 atcggctcca ctgaagcctg atggtatcca tcatccgtcc caacgcgtgc catgcgggag
      181 acccgctgtc gatactttcg atcgaaggag gagtagggtc catcgagacc cacccggaat
      241 gcggtaggtg cacctctacc cacgcgtggg cgtcttgctg tctgaccaaa tagtagttgc
      301 cgtattcgtt ccactcggtg gcgagaaacc ccgtgacgag gcgcgccggc actccgattg
      361 cccgaagcat aatcaccata gcggtggcgt agtgttcgca gtagccggtc ttgcgggtga
      421 agagaaactc ttcaagcggg cggtcctgcc ctgtcagagg cgcatcaagg ctatagcgaa
      481 agttgtgtgt caggtacgat tggatggcat tggctttatc atacatcgtg cgttgtgtgt
      541 cagtgatttc ctttgccagg atagcgatcc gctcggattg agcggggatc tgcaagaaat
      601 gtcggataaa tgattcagaa tagcggctgg gttccaaacc gagatcagcc ggtagtaccg
      661 gattggatcg ggaaaggacg gaatactcga tccgcgacga actggggaac ggtaaataaa
      721 ccgaaccggt tagatcggac tggacactcg ggaactttcc gctgactctc tcgataaaag
      781 gggccgcgaa gagaaccggg gtgtcgagcg gttccaaaag aatattctgc cggatggtta
      841 cgccaagacg ggccggtgca ggagatcgac caccccgaag aacaaacgtc ccagtcgtgt
      901 cttcgctcac actccgccgg tggttcaacc gattcgtcca ggtcttgccg tcgtattgat
      961 cgaatgcaac acctcgtagg tacagcggac ctgtttcgtg cggggagcca tcagacaact
     1021 ccacccgcat cacgacgctg ggatctcgct tgatgggccc aatctcacct aagttcaccg
     1081 tttcggagaa tcctgacgtg cgaatgtttt ccccaaagcc tttttggtag aaccctgcac
     1141 tgacccgggg aatggtaaag aagatcacca acgtcagccc aaaggttgca aaggcaagcc
     1201 cgtttgcaag ccaaaagagt tgtggcgtta cttggttgac cgatgcaggg ggctgtgggc
     1261 gcatggggac cgacatcgcg agcatccgag tttcctcctg atgcttggtc agctgaaata
     1321 gtaacagcgt ccacacaccg gccaaaagat agagcaggaa aatggggagg taccacagat
     1381 ccgtcgtgag ggacccggaa gccaggatcg ccacgaggct gatggcgtag agatgcagat
     1441 aatcacgacg aagcttgagg ttgaacagct tgatgatcat gaggatcata aggaagtgaa
     1501 ttcctgccgg aaggagttca cccgagatcc ataacatatc cacccagaat ccaaggaagc
     1561 cgacgatcac aagaagattc catccggcgg ctgaaattga cgcaaacgtc gcaagccgtt
     1621 ccactgactt gccccctctg ttccgtatga gagcaacgat cagcgcggct ccggtcagcg
     1681 cagccagcca ttccggcaga ccggctccga gcgtcaatcc gatgaaggac atggatgcca
     1741 gccaaatcga tgcgaaccga agggcctgat caaacggcat gtgatgctcc ggtgatgacg
     1801 gctccattga tcaggatggt ggggtgttcc cgctcgatat ccatcggtcc cagccatgaa
     1861 tgcacgacaa tcatcgcgat cccctcgtgc tctaggtttc tggacaattc tcccggtacg
     1921 gcggactctg ccagggggaa gcaccgctca cagagggcga gcattcgaag aagttccaag
     1981 agatggaggt cgccttgacc aaatgacgat tgcgacgacc ccaccatca
//
LOCUS       DASTWE010000344         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_687813, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000344 DASTWE010000000
VERSION     DASTWE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687813"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFT30_03655"
     CDS             <1..222
                     /locus_tag="VFT30_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091751.1"
                     /translation="LFMQLAEGVDEKTWLHHLRQGDYSKWISEGIRDSHLADAIRRIE
                     QQPSLSAERSRQMIRSAIEERYTVPATGV"
     gene            223..522
                     /locus_tag="VFT30_03660"
     CDS             223..522
                     /locus_tag="VFT30_03660"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5091752.1"
                     /translation="MRKAFRGWFMISSILFFSAALVGCGLGGRAADEVLAQKVKHALY
                     DHGEANLLRVDVSVEQGVVYLSGETDDGQQKHTAEQIARRIAGGAKVMNKVLVEP"
     gene            562..849
                     /locus_tag="VFT30_03665"
     CDS             562..849
                     /locus_tag="VFT30_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEU5091753.1"
                     /translation="MHVAHWTIIGVATIGLIGGCEMHHDSKRDLLRQSNVSLIDAVRM
                     AEASITGGKTIEAELEREEGRPVYEVKMIDSTQNKRKVHVDAATGKVTKID"
     gene            858..1994
                     /locus_tag="VFT30_03670"
     CDS             858..1994
                     /locus_tag="VFT30_03670"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5091754.1"
                     /translation="MRPRALSSLLPALIVWGLSSPIHAEVREAVHIRAILDLSYGVNA
                     NFPDNHTFRSKQTTPQANEVAPNLGFLSIRREVTRESRWGGELGFQAGYDTNGLVPDE
                     REKPLAGSDILRHLAQANVTYLAPLGKGLAISAGLMKGFINFESFYAVNNFNYTRAYL
                     TDYSPNFIFGVGSRYPITENVDVGFHVLNGFQHLAHPNGLPSYGAELDWRIARRWVVA
                     QNVYYGPDQEDTSIRYWRIFSDTQLQWRGEDWTVVAAFDIGTERIAEQISAPRSFWTG
                     AALFTRWEVGGPWSLAVRPEFFWDRNGRLTRAEQLIWAVTSTLEYKRNWGDHTGIVRL
                     EHRFDRSTGVDGGFFRGSLTGTGQPALVPNQHLILLSLLWAFDS"
     gene            complement(2158..>2440)
                     /locus_tag="VFT30_03675"
     CDS             complement(2158..>2440)
                     /locus_tag="VFT30_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5091755.1"
                     /translation="KVPGEALVGERIELVARQCSGDEMDPYERLLGDAMKGDATLFAH
                     RDGVEAAWHIVQSILETPMSLHEYEPNTWGPREADRLIEGSWHNPQTSG"
BASE COUNT          535 a          626 c          711 g          568 t
ORIGIN      
        1 ctcttcatgc aactcgcgga gggtgttgac gaaaagacgt ggttacatca ccttcgacag
       61 ggggattaca gcaaatggat atcggagggc attagagatt cccatttggc cgatgccatt
      121 cggcggattg aacaacagcc atccctcagt gccgagcgca gtcgacaaat gatacgttct
      181 gccatcgagg aacggtacac agttcccgcg actggtgtgt aaatgcgcaa ggcgttccgc
      241 ggttggttca tgatctcgtc gattctcttt tttagtgcag cccttgtcgg gtgcggtctg
      301 ggcgggaggg ctgcggacga agtacttgcg cagaaggtga agcacgcctt atatgaccat
      361 ggtgaggcga acttgctccg cgtggatgtg tcggtcgagc aaggagtcgt ctatttgagt
      421 ggagaaacgg acgacgggca acaaaaacat accgcagagc aaatcgcgcg ccgcattgca
      481 ggcggtgcca aggtcatgaa caaggtgctc gtagaaccgt aaaatttgtg tcccgttaca
      541 tttagagcca aggaggacgc catgcatgta gcccattgga cgatcattgg agttgcgacc
      601 atcggtttaa tcggtggatg cgaaatgcat cacgactcga aacgagattt actcaggcag
      661 tcgaatgtct cgttgatcga tgctgtacgc atggctgagg ccagcatcac aggcggaaag
      721 accattgaag ctgaactgga gcgggaggag gggcgacccg tttacgaagt aaaaatgatc
      781 gactcgacgc agaacaagcg taaagtacac gttgatgccg ccacaggaaa agtcacgaag
      841 atagattagt cgacgccgtg cgcccgcgtg cgttgtctag tctcttaccc gcgctcatcg
      901 tttgggggct gagctctccc atccatgctg aggtccggga agcggttcac atcagggcca
      961 tcctggatct tagctatggc gtgaatgcca atttccccga taatcatacg tttcgcagca
     1021 agcaaacgac gcctcaagcg aatgaggttg caccgaatct tggatttctc tctatccgac
     1081 gtgaggtcac gagggagagt cggtgggggg gcgagctggg attccaagcc ggctatgaca
     1141 caaacggtct cgttcccgat gaacgggaaa aacctttggc cggctcggac attcttcgtc
     1201 atctggcgca ggcgaatgtg acgtatctgg ctccgcttgg taaaggcctc gcgatttccg
     1261 cggggctcat gaaaggattc attaattttg aatcgttcta tgccgtcaat aatttcaatt
     1321 atacccgtgc ctatctcacc gactactctc cgaacttcat cttcggagta ggcagtcggt
     1381 atcccatcac ggaaaacgtc gatgtggggt ttcacgtgtt gaacggcttt cagcacttgg
     1441 cgcatcccaa cgggcttccc agttatggag cggagctgga ttggcgaatc gcccgccgct
     1501 gggttgtcgc gcagaatgtc tactatgggc cggatcaaga ggacacgtcg atcagatact
     1561 ggcgcatctt ttcagacacg caactgcagt ggcggggcga agattggacg gtggtcgcgg
     1621 cattcgatat cggtaccgag cggatcgcgg aacagatatc cgcgccccgt tcattctgga
     1681 ctggggcagc attgttcact cgatgggaag ttgggggacc gtggagtttg gcggtgcgac
     1741 cggaattctt ttgggatcgc aacggccggc tcacgagagc cgaacagctg atctgggctg
     1801 tgacgtccac cctggagtac aaacggaatt ggggcgacca taccgggatt gttcgtctcg
     1861 aacaccgctt tgatcgctcg acaggcgtgg atggtggatt ctttcgcgga agtttgacag
     1921 gaaccggtca accggcgctc gtccccaacc aacatctgat actcctgtcg ttactatggg
     1981 catttgattc ataaattatc gactaggggc gcgctgttcg tggcgggtcc cggtgtctag
     2041 tttttgggat caccgagatg ttggtctccg acttccatcg cggcatattt gatctgttcc
     2101 attaccagga agagcggctt atgttcggca cttcgaaggg gattcctcaa gcgtgattca
     2161 tccggacgtt tgcggattat gccaactacc ctcgatgagc cgatccgcct cacgcgggcc
     2221 ccaggtgttt ggttcgtatt cgtgcaatga catcggggtt tcgagaatcg actgcacgat
     2281 gtgccaagcg gcttccaccc cgtccctatg ggcaaagagt gtggcatcac ctttcatggc
     2341 gtctccaagt agacgttcat atggatccat ctcatcgccg gagcattgcc gtgcaaccag
     2401 ctctattcgc tctcccacca gcgcctctcc cggaactttg
//
LOCUS       DASTWE010000345         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1108145, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000345 DASTWE010000000
VERSION     DASTWE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108145"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFT30_03680"
     CDS             <1..649
                     /locus_tag="VFT30_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU5091756.1"
                     /translation="LDAKAFAAALPQAVPGDLVLFGADKASVVHDVMGRIRLLLGEEL
                     NLIDKTIWKPLWVTDFPLLDYSQEEKRYVAMHHPFTAPLDEDLPLFESDPLKMRAKAY
                     DMVLNGSEVGGGSIRIHRRDVQSKVFDLLGIGKDEASAKFGFLLEALEYGAPPHGGIA
                     FGLDRLVMLLGKADSIRDVIAFPKTQKAQCPLTDAPSAVSPDQLKELRIKLDMVE"
     gene            666..1778
                     /gene="aroC"
                     /locus_tag="VFT30_03685"
     CDS             666..1778
                     /gene="aroC"
                     /locus_tag="VFT30_03685"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5091757.1"
                     /translation="MAGNTFGHIFTVTSFGESHGPAIGCIVDGCPPGLSLSTEDIQKD
                     LDRRKPGTSRHVTQRQESDTVEILSGVFEGKTTGTPIALLIRNEDARGRDYGNLIDTF
                     RPGHADYTYWQKYGIRDHRGGGRASARETAVRVAAAAIARKWLSEKYGVVIRGYLSQL
                     GPHELAFKDWNAVGQNPFFSADPDVVPKLESFMDDLRKAGDSVGARITTVAEHVPVGW
                     GAPVYRKLDAELASAMMSINAVKAVEIGSGFASVVQRGSEHGDELTPDGFVTNHAGGI
                     LGGISTGQDIVVTIGIKPTSSIRIPRRSIDKQGNPVMVETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNAEVATKTPKIAASVKRTTL"
     gene            1905..3200
                     /gene="rnhA"
                     /locus_tag="VFT30_03690"
     CDS             1905..3200
                     /gene="rnhA"
                     /locus_tag="VFT30_03690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEU5091758.1"
                     /translation="MMEDNRIIEIYTDGACSGNPGPGGWGVLLRIGDAETELCGGEPG
                     TTNNRMELLAVIEALQSFTESVEARVYTDSQYVQKGISEWIHSWKQRGWKTAGKESVK
                     NEDLWRRLDALASKHRLEWRWVRGHNGHPENERADALARAGLERARRVGQPVPGQITL
                     PVQEPSRSDGSASILDIEHAMVYRGDTCVFSDFSFALRGGEHAAILGPNGAGKSTLLK
                     LLSGEVHAMPKDETKIVLFGEARWNVWDVRKQIGIVSHDLQRDYLICAEGLNVILSGF
                     YASNDTYEYQQFTKTQITRAYEVMQELGIMALSDRRFGHLSTGEQRRFLLGRALVHDP
                     QVLVLDEPTSGLDLKACFQYLDLLRAQIAKGKTVLLVTHHLHEIPPEIDRIIFLKEGK
                     ILADGFKSALFTNDQVSRLFDSKITLVQANGWYQALPGS"
BASE COUNT          809 a          911 c         1044 g          807 t
ORIGIN      
        1 tttggatgcc aaggcatttg ccgcggcatt gcctcaagcc gtgccggggg acctggtttt
       61 attcggcgcg gacaaagcct cggtcgttca cgatgtgatg gggcggatta gacttttgtt
      121 gggtgaagaa ctgaatctca tcgacaagac aatttggaag ccgctttggg tgacggattt
      181 tccgctgctt gactattctc aggaagagaa gcggtatgtg gccatgcatc atccattcac
      241 agcaccgctt gatgaagacc tgccgctgtt cgagtccgat cctctcaaaa tgcgtgcgaa
      301 ggcctacgac atggtgctga acggcagcga ggtcggtggc ggcagtattc gtatccaccg
      361 gcgcgatgtg caaagcaaag tgttcgatct cctcgggatc ggcaaggacg aagcatcggc
      421 taagtttgga tttctgctcg aagcgctcga gtatggtgca ccaccgcacg gtggaatcgc
      481 gtttggtttg gatcggttag tcatgctgct cggcaaggcc gactccatcc gcgacgtcat
      541 tgcatttccc aagacccaga aagcgcaatg tccgctcacc gatgcgccgt ctgcggtgag
      601 tcccgatcaa ctcaaggaat tgcgcattaa gctagacatg gtcgaatagg ggagctcgag
      661 tccgcatggc cggcaatacg ttcggtcaca ttttcacggt tacctcgttc ggtgaaagtc
      721 acggaccggc aattggatgt atcgttgatg gttgcccacc gggtctttcg ctttcgactg
      781 aagacattca aaaagacctc gacaggcgca agccaggtac ctcgcgccat gtgacccagc
      841 ggcaggagtc ggataccgtt gaaattctct ccggagtatt cgagggaaaa accaccggga
      901 cgccgatcgc gctgttgatc cgcaatgaag acgcgcgagg ccgagattac ggcaatttga
      961 tcgatacctt tcgtcccggc catgccgact acacgtactg gcagaagtac gggattcgtg
     1021 accatcgagg cggaggccgt gcatcggcgc gtgagactgc ggttcgagtt gctgccgctg
     1081 ccattgcgag aaagtggctc agcgagaaat atggtgttgt gattcgcggc tatctaagcc
     1141 agttgggtcc tcacgaattg gccttcaaag attggaacgc agtgggacag aatccgtttt
     1201 tctccgctga tccggatgtt gttccgaagc tggaatcatt catggatgat ctgcgaaagg
     1261 cgggcgattc ggtcggtgcc agaatcacaa cggtggctga gcatgtgccg gtgggatggg
     1321 gcgcgccggt ttatagaaaa ttggatgccg aattggcctc agcgatgatg agtatcaatg
     1381 cagtgaaggc ggtcgagatc ggatcaggct tcgcgtcggt tgtccagcga ggatctgaac
     1441 atggcgacga actcacgccc gatggctttg tgacgaatca cgcgggtggc attctgggcg
     1501 gtatctccac cgggcaagac attgtcgtga cgatcggcat caagccgaca tcaagcatcc
     1561 gcatccctcg ccgatcgatt gataaacagg gcaatcccgt catggttgag accaatggac
     1621 gccatgatcc ctgtgtcggg attcgcgcca ctccaattgc ggaagccatg atggcgctcg
     1681 tgctcatgga ccatgcgttg ttgcatcgcg cccaaaatgc cgaggtggcg acgaagacgc
     1741 cgaaaattgc cgcttctgta aaacggacga cattataaga caacataaga cctgcctcga
     1801 agatgaatcc agatcctgcc gaagcatagt ggatgtgggt gtgtcggaac tgatccaact
     1861 ctcatcggtt aatgtgaatt gagcgaacgg catagccggt acccatgatg gaagacaatc
     1921 ggatcatcga aatctataca gatggtgcct gcagcgggaa tcctgggccc ggcggctggg
     1981 gggtcttgct gcgtatcggc gatgctgaaa cggaactctg tggcggagag ccaggaacaa
     2041 cgaacaatcg gatggagctg ctcgccgtca ttgaggcatt gcaatcattc acagagtcgg
     2101 tggaggcacg ggtctacacc gactcgcaat atgttcagaa aggcattagt gaatggattc
     2161 atagttggaa gcagcgcggg tggaagacag ccgggaagga gtcggtgaag aatgaagact
     2221 tgtggcggcg gctcgatgcg ctggcttcca agcatagact cgaatggcga tgggtcagag
     2281 ggcataacgg acacccggag aatgagcggg cggacgcgtt ggctcgcgcc ggtcttgagc
     2341 gggctcgccg agtcggtcaa ccagtcccgg gccaaattac tcttccggtc caagagccga
     2401 gccggtcgga cggttcagcc tcaatccttg atatcgagca tgccatggtc tatcgaggag
     2461 acacctgcgt cttctcggat ttctccttcg ctttgcgggg gggggaacat gcggccattc
     2521 ttggacccaa tggagcgggg aagtcgacac tcctcaaatt gttgtcaggc gaagtacacg
     2581 caatgccgaa ggacgaaacg aagatagtgc ttttcggcga agctcggtgg aatgtctggg
     2641 atgtgcggaa acaaatcggc attgtgtcgc acgatctcca gagggactac ctgatctgtg
     2701 ctgaagggct caacgtgatc ctttccgggt tctatgcgag caacgatacc tacgaatacc
     2761 aacaattcac caaaacacag ataaccaggg cgtatgaggt gatgcaggaa ttgggaatta
     2821 tggctttgtc tgaccgccgc ttcggccatc tatccaccgg cgagcagcga cggtttctgt
     2881 tgggccgggc gctggtgcat gacccgcagg tactggtgct ggatgaaccg acaagcggtc
     2941 tcgatctcaa ggcatgcttt cagtatctcg atttgctgcg ggctcaaatc gcgaagggaa
     3001 agactgttct gcttgtgact caccatctgc atgaaattcc gcccgagatc gatcgcatca
     3061 tttttctgaa agagggaaag atcctcgctg acggtttcaa atccgctctg tttactaacg
     3121 atcaagtcag ccgactcttc gacagtaaga tcacactcgt tcaggccaac ggctggtacc
     3181 aagccttgcc agggtcataa ccgaggcctt cctcaacttt tccacaatcc gcactttttt
     3241 tgacgaacct cattttgcgc gataggcttc agtgctggta tttgcttgtc agcagatatg
     3301 tatcaaacca tattgaaaca tacgttctct cttcctttca ttttttacct gtgcgcaatg
     3361 gcgtatgcta ttcccgactc ggcgtgggcc gaaacctggg tctgccctcg acctgggcag
     3421 tcggatctgt atacagatcg tagcgggcca ggctgcagac aactgggcga ggccaagact
     3481 tactctccat tggaaatttc tccggctcgt aagtcacctc agtcgactca tgcgttacca
     3541 tcggtgtcga acaacgtctc ttccggaatc t
//
LOCUS       DASTWE010000346         1173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_590838, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000346 DASTWE010000000
VERSION     DASTWE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590838"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1173
                     /locus_tag="VFT30_03695"
     CDS             <1..>1173
                     /locus_tag="VFT30_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091759.1"
                     /translation="GFETNLSRKDRQEANIDKSLELHGYRETMGYAYSSGPSLGTAMA
                     ISGAAANPNSGYHTSAPMAFLLTVFNVRLGWWLGNPRRRGPSKEPGPRFALWYMFADL
                     FAQSNSRSHFINLSDGGHFDNIGLYELIRRRCRYIIVGDSEEDGKLTFSALGDAIRRC
                     RADFGVEIDLDPTPVQTKVNGFSKKHCVVGTIHYPEQETGIYAGVCDGQRAAEPGDHA
                     RGWILYIKSSLTGDEPSDVLEYQSRCPNFPHQSTGDQFFSESQFESYRRLGLHIGRDA
                     FEEIDRGSSGSHNDLLNLFQGLARKWYAPIPVSEEAATRLANEYSALVRRIGEDENLR
                     AVFGSLLQEQPPGATPPAVNELATSFGLELVQHMENVFTEFRFEHGLNRANPRNAG"
BASE COUNT          268 a          329 c          333 g          243 t
ORIGIN      
        1 ccggattcga gaccaacctt tcgcggaaag accgccaaga agcaaacatc gacaagagcc
       61 tcgaattgca cgggtatcgc gaaacgatgg gctacgcgta ttcgagcgga ccaagtctag
      121 ggactgctat ggcaatctcg ggagccgcgg cgaacccgaa tagcggctat cacacgtccg
      181 ccccgatggc gtttctgttg acagtattca atgtgcgact cggctggtgg ctcggtaatc
      241 cgcgccgccg tggtcccagc aaggaacccg gaccgagatt tgccttgtgg tatatgtttg
      301 cggacttgtt tgcgcaaagc aatagccgca gccatttcat caatctgtcg gatggtgggc
      361 acttcgataa tatcggactc tatgaactca tccgcaggcg atgtcgctac atcatcgtcg
      421 gggactcaga agaagacggc aagctgacgt tttctgcctt gggcgacgcg attcgcaggt
      481 gtcgcgcgga cttcggtgta gagatcgatc tcgacccgac tccagtccaa acgaaggtga
      541 acggcttcag caaaaaacac tgcgtcgtcg gcacaatcca ctatcccgag caggagacgg
      601 gaatctatgc cggagtatgc gacgggcagc gcgctgccga accgggcgat cacgcgagag
      661 gctggatttt gtacatcaag tcaagcctga ctggagacga gccgtcggat gtcctggagt
      721 atcaatcccg ttgcccgaat ttcccacatc aaagcaccgg tgaccagttc ttttctgaat
      781 cgcaatttga aagctaccgt cgcctgggtc tccatatcgg ccgcgatgcg ttcgaagaga
      841 tcgatagagg atcgagcgga tcccataatg acttgttgaa tctctttcag ggccttgcgc
      901 gtaagtggta cgccccaatc cccgtatcag aggaggccgc gacacgcctt gcaaatgagt
      961 attcggcact cgtacgacgc atcggtgaag atgagaacct gcgggccgtg ttcggcagcc
     1021 tgctccaaga gcaaccgcct ggcgctaccc cgcctgctgt caacgaactc gccacctctt
     1081 tcggattgga acttgtgcag cacatggaga acgtgttcac cgaattcagg tttgagcatg
     1141 ggctgaaccg tgcaaatccc cggaatgccg gct
//
LOCUS       DASTWE010000347         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_946381, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000347 DASTWE010000000
VERSION     DASTWE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946381"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFT30_03700"
     CDS             <1..528
                     /locus_tag="VFT30_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091760.1"
                     /translation="KEGLRMKRAIVLAAVAVFLGFGLLAAEVSLAADVEKLGLADAVV
                     GGKPLKSEAAAKTLQGRVWSQWADNPDGELLFGIQYWNSGEPGSGTPGGRSSTMLDVK
                     PPDPLFSCCAWGFTGGSEKNSTYSGWYHAAATVRLAVKDKALMDQIIKASQELVAMEV
                     TLDGRTITGFKVIKD"
     gene            570..1232
                     /locus_tag="VFT30_03705"
     CDS             570..1232
                     /locus_tag="VFT30_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091761.1"
                     /translation="MMLQKRGAAVLAAVALVVGMGSTVSAIESFQERFEWGDMSKPTT
                     LQGRVIILDPYDEAVWINLAVFGGNAESGLYWQKVHPGKTLKFYADKSAWQELKKMGR
                     THAGPAAAKEVPPGSTTDLIEFVVTETEQNHRVISSVKKIPEVSGAMGKPLSISALRL
                     KDCAGKSEFDKACAAAKQGLNTSPMGPDGGSVGMQYDVMLPGGKTVGGLIPWTAQYNQ
                     AH"
     gene            complement(1312..>2074)
                     /locus_tag="VFT30_03710"
     CDS             complement(1312..>2074)
                     /locus_tag="VFT30_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5091762.1"
                     /translation="ITGPNMAGKSTYLRQVALIVLMAQIGSFVPATDAHIGLTDRIFT
                     RVGASDNLAGGQSTFMVEMVETANILQHATQRSLILLDEIGRGTSTYDGLSIAWAIAE
                     HIHDRARLGARTLFATHYHEMTQLEQQRAGIKNYRVAVQECGGDVVFLRKIVAGKADR
                     SYGIHVAKLAGLPTAVISRAQAVLSQLEQPELARSWLIQEQPSQTSEPLPQPHPIIEE
                     VKQIDLFSMTPLDALNRLAELQRMVRSDSSDPSDR"
BASE COUNT          475 a          522 c          602 g          475 t
ORIGIN      
        1 aaggagggat tgcgtatgaa aagggctatc gttctagctg ccgtcgcggt atttttgggg
       61 ttcggcttgc tagcggccga agtttcccta gccgccgacg tagaaaaatt aggcctcgct
      121 gatgcagtcg tcggggggaa accactgaag tctgaagccg cggccaagac actgcaaggc
      181 cgtgtctggt cgcagtgggc ggataacccg gatggagaac tcttgttcgg gattcaatat
      241 tggaattcgg gagagccggg gtctggcacc ccaggtggtc ggtcctctac catgctggac
      301 gtgaaaccgc ctgatccctt gttcagttgt tgtgcctggg gttttaccgg cggcagtgag
      361 aaaaattcaa cctattcagg ctggtaccat gcggccgcga cagtccgtct ggccgtcaaa
      421 gataaagcgc tgatggacca aatcatcaag gcctctcaag aattggtcgc tatggaggtc
      481 acgctggacg gcagaaccat caccggcttc aaggtaatca aggactagcc actctcaatg
      541 tggaagatgt tgttgaagga ggaccaatca tgatgttgca gaagcgaggt gcagccgttc
      601 tggcagccgt tgcactggta gtcggtatgg gttcgactgt ctcggccatt gagtcatttc
      661 aagagcggtt tgaatggggt gatatgagca agccaaccac tcttcagggt cgggtgatca
      721 tccttgatcc ctacgatgaa gcagtatgga tcaacttggc agtcttcggt ggcaatgccg
      781 aaagcgggtt gtattggcag aaagttcatc caggcaaaac actgaagttt tatgccgata
      841 agtctgcctg gcaggagctg aagaagatgg ggcgaactca tgccggaccc gcagctgcga
      901 aggaagttcc cccaggcagc acgacggact tgatcgaatt cgtcgtcacg gagaccgaac
      961 agaatcatcg ggtgatttca tcagtgaaga agattcctga ggtctcaggt gcgatgggta
     1021 agcccttgag catttccgcg cttcgtctca aagattgtgc cgggaaaagt gaattcgaca
     1081 aggcttgcgc agcagccaaa caaggactca acacttcacc gatgggtccc gacgggggca
     1141 gtgtaggcat gcaatacgat gtgatgcttc caggcggaaa aaccgtcggt ggtttaattc
     1201 cctggaccgc gcagtataat caagcgcact gaaggctctt ccagatgaaa acgggcggta
     1261 ttcccttggg gaatgccgcc cgtttcattt caccaacaga tccatgagta gttatcgatc
     1321 ggatggatca ctgctatccg atctcaccat gcgctgcaac tcggcaaggc gattcaatgc
     1381 atcaagcggt gtcattgaaa acaagtcgat ctgcttgact tcctcaatga ttggatgagg
     1441 ctggggaagg ggctcagacg tctgggaggg ctgttcctga atcaaccagg aacgagccaa
     1501 ctccggttgt tcaagttgcg acaacacagc ttgcgcccga ctgatcacag cggtagggag
     1561 cccggcaagt ttagcgacgt ggataccata actccggtcg gcttttcccg ccacgatttt
     1621 tctgagaaac acgacgtccc cgccgcattc ctgaacggcc acgcgataat tcttgattcc
     1681 ggcccgttgc tgctccagct gtgtcatctc atggtaatgg gtcgcgaaca acgtgcgtgc
     1741 acctaatcgt gcgcggtcat gaatgtgttc tgctatcgcc caagcgatgc tcagaccatc
     1801 gtaggtgctt gtgccgcggc cgatttcgtc caacagaatc aggctacgtt gagtagcgtg
     1861 ttggagaatg ttggctgtct cgaccatttc aaccatgaac gtactctggc cccccgccag
     1921 attatccgag gctcctactc ttgtgaagat gcgatcagtc aatccgatat gcgcgtctgt
     1981 ggcgggtacg aagctgccga tttgggccat cagtacgatc agtgcgacct gccgaagata
     2041 ggtgctcttc cctgccatgt ttgggccggt aatg
//
LOCUS       DASTWE010000348         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_429433, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000348 DASTWE010000000
VERSION     DASTWE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429433"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFT30_03715"
     CDS             <1..484
                     /locus_tag="VFT30_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5091763.1"
                     /translation="ESDGLFRRVAEETAKGYPEVIYNQYIVDDFARRIVASPQEFDVV
                     VMPNLYGDILSDAAAGTIGGLGLAPSGCYGENYAYFESIHGTAPDIAGKHLINPTATL
                     LSAIMMLEYIGYTDAAARLDHAVSQVYSDGKTLTLDQGGSASTFAFCTAVRECLGKNR
                     "
     gene            complement(515..670)
                     /locus_tag="VFT30_03720"
     CDS             complement(515..670)
                     /locus_tag="VFT30_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091764.1"
                     /translation="MSENELVKQLKQWRETRTRKVPQQSTSDRRMVRVNGQEVVVITK
                     ARGKAQG"
     gene            complement(708..>1132)
                     /locus_tag="VFT30_03725"
     CDS             complement(708..>1132)
                     /locus_tag="VFT30_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5091765.1"
                     /translation="LGREPSPEEIATQMEVPADKVRKVLKIVKEPVSLETPIGDDEES
                     SLGDFVEDRQTLSPADAAMALSLEEQTRKVLATLTPREEQILRMRFGIDEKTDYTLEE
                     VGQRFAVTRERIRQIEAKALRKLRNPSRAKSLEAFIDA"
BASE COUNT          236 a          323 c          292 g          281 t
ORIGIN      
        1 cgagagtgac gggctgttcc gccgcgtcgc cgaagaaacc gcaaaaggtt accccgaagt
       61 catctacaat cagtacattg tcgatgactt cgctcgtcgg attgtcgcga gcccgcagga
      121 gtttgacgtc gtggtaatgc cgaatctcta cggcgatatt ctttccgacg ctgccgcagg
      181 cacgatcggc ggtctcggcc tggcaccgag tggatgttac ggtgagaact acgcctactt
      241 cgagtcaatc catggcaccg ctcccgacat cgccggtaag cacctcatta atcctacagc
      301 aaccttgttg tccgcgatca tgatgctcga atacatcggc tacaccgatg cggccgcacg
      361 gctcgatcat gcggtatcgc aggtgtattc cgacggaaaa acactgaccc tggaccaagg
      421 tggatcagca tcaacctttg cgttctgtac ggcagtacga gaatgtctgg gaaagaaccg
      481 ctaaggaaga aatagacagg gatgggacga atgcctatcc ctgcgccttg ccgcgggcct
      541 tggtaatgac caccacttcc tgaccattca cacgaaccat ccgacgatca gatgtacttt
      601 gctggggaac tttgcgggta cgggtctccc gccattgttt gagttgtttc actagttcgt
      661 tttcgctcac agatttcctc cggccggtcg gtgtgaccgc cttgttgtta cgcgtcaata
      721 aacgcttcta aactcttggc acgagatggg ttgcgcagtt tccgcagggc tttggcttcg
      781 atttgacgaa tgcgttctcg cgtcaccgcg aaacgttgtc cgacttcttc taacgtgtag
      841 tcggtctttt catcgatccc aaaccgcatc cgtaagattt gctcttcacg gggagtaaga
      901 gtcgcaagca ccttgcgagt ctgctcctcc agactcagtg ccatcgccgc gtccgccggt
      961 gacaatgttt gccgatcctc gacaaagtct ccgagcgaac tctcttcatc atcaccaatt
     1021 ggtgtttcga gcgacaccgg ttctttgacg atcttgagga ccttgcgtac tttatcggca
     1081 ggaacttcca tttgtgtagc aatttcctcc ggggagggtt cccgacccaa tt
//
LOCUS       DASTWE010000349         1253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_9435, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000349 DASTWE010000000
VERSION     DASTWE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9435"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1253)
                     /locus_tag="VFT30_03730"
     CDS             complement(<1..>1253)
                     /locus_tag="VFT30_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5091766.1"
                     /translation="AMHNALLFSRRINLPPVATNRVYFTDQAGFATHRLLRTIALNTT
                     LSRLPKEACCTSRQWLMPPVLMASYFPHVPEALENTVRIAEACYSDWRFGETIFPAFR
                     QLTDEEAFLTLKDKTYVGAHHRYGVITQEIRDRIEKELAIIREKRFAHYFLVVEEIVK
                     TSKGTTCGRGSVAASIVSYCLNITHVEPIKHTLFFERFLNPGRKDPPDIDIDFAWDER
                     DDILKWVFEQYGERQAAMVANQNSLGFRAAIREVAKVSGMPGEEIGRISSRVIRQKDL
                     LGFSTLPTDEQWLHRLSQTLQLKAPWPEILAQALKAQNHFRHLSMHCGGVVIVPDEIR
                     RYVPIEITAKGLPVIQWEKDQTEDAGLVKIDILGNRSLAVIRDALTAIAKHTGRTIDY
                     KTWDPLHDAATQDAIRCGNTIGCFY"
BASE COUNT          292 a          306 c          356 g          299 t
ORIGIN      
        1 atagaaacag ccgatcgtat tgccgcatcg gattgcgtct tgcgtggcgg catcgtgaag
       61 cggatcccag gtcttgtagt cgatcgtcct gcccgtatgt ttggcgatgg cggtgagagc
      121 atctcgaatc acagcgagcg accggttgcc caagatatcg attttcacga gccctgcatc
      181 ttccgtctgg tccttttccc attggatgac gggcagaccc ttcgccgtga tctcgatcgg
      241 cacatagcgc cggatttcat ccggtacgat gacgacacca ccgcagtgca tggaaagatg
      301 acggaaatga ttctgcgcct tcaatgcttg cgcaagaatc tcgggccatg gtgctttcaa
      361 ctggagggtt tgagacaacc gatggagcca ttgctcgtcg gtgggtaaag tggaaaagcc
      421 aaggagatct ttttgacgta taacgcgtga agatatcctg ccgatttctt cacccggcat
      481 gccggacacc ttggcgactt cgcgaatggc ggctcggaac cccagactgt tctgattcgc
      541 caccatggcg gcctggcgct cgccgtactg ctcgaatacc catttcaaaa tgtcgtcacg
      601 ctcatcccat gcaaaatcga tatcgatgtc cggtggatct ttccgaccgg gattgagaaa
      661 ccgttcaaag aagagggtgt gcttgatcgg ctcaacatgg gtgatgttga gacagtagga
      721 gacgattgag gcagcgaccg aaccgcgtcc acaggtggtt ccctttgaag tcttgacgat
      781 ttcctccacc acaagaaaat aatgagcgaa gcgtttttct cggatgattg ccaactcttt
      841 ttcaatccgg tcacgaattt cttgtgtgat gacaccgtat cgatgatgtg cgccgacata
      901 ggttttgtct ttgagtgtca aaaacgcttc ttcatcggtc aactgacgaa aggctgggaa
      961 gatagtctcg ccgaaacgcc agtcgctgta acaggcctcg gcaatgcgaa cggtgttttc
     1021 taacgcttca gggacatgtg gaaagtaaga tgccatgagg acgggtggca tcagccattg
     1081 tctggacgta caacaagctt ctttcggcag tcgtgacaaa gtcgtgttga gggcgatggt
     1141 gcgcaacaga cgatgtgtgg caaatccggc ctgatcagta aagtacacgc gattggtggc
     1201 aacaggtgga agattgatac ggcggctgaa gaggagggca ttatgcatag cgg
//
LOCUS       DASTWE010000350         1309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_623191, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000350 DASTWE010000000
VERSION     DASTWE010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623191"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFT30_03735"
     CDS             <1..367
                     /locus_tag="VFT30_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091767.1"
                     /translation="AQRDLFAIGVDSNQDDIQPGHVIVSSLKKVESTTFDPIKLVVDG
                     TFTGGFQRRGLKEGFAGLSWDEGSTTFEQQGPADMTAKIPEIKTLVEEYRTKILNGEY
                     KVCDAFTPETPECVELLAK"
     gene            609..>1309
                     /locus_tag="VFT30_03740"
     CDS             609..>1309
                     /locus_tag="VFT30_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5091768.1"
                     /translation="MERGEIHALVGENGAGKTTLMNILYGLIKPDSGDILIDSQNVRI
                     DNPDDAIRHGIGMVHQHFKLVSSFTVAQNILLGMEPQQAGFLDQQKENELVDQLAKRY
                     NLPVNPAARIRDLPVSMQQRVEILKVLERNAKILVLDEPTAVLTPQEVNELITVIRKL
                     SAQGYTIIFITHKLIEVMSVADRVSVMRGGKMIGTKKVSETSIPELARMMVGREVLLR
                     VKKEERPRGDVVLDV"
BASE COUNT          330 a          317 c          385 g          277 t
ORIGIN      
        1 agcccagcgt gatctcttcg cgatcggcgt cgactcgaat caggacgata tccagcccgg
       61 gcatgtcatt gtctccagtc tcaagaaagt ggaatcgacc accttcgacc cgatcaaact
      121 ggtggtggac ggcacattta ccggcggatt ccagcggcgc ggcttaaagg aaggctttgc
      181 cggtctttcg tgggatgaag gctccacaac ttttgaacag cagggtccgg ccgacatgac
      241 ggcaaaaatc cccgagatca aaaccctggt ggaagaatat cggacgaaga tcctgaatgg
      301 ggaatataaa gtctgtgatg ccttcacgcc ggagacgcca gaatgtgtgg aactgctcgc
      361 caaatagctt gaacagcatt gtatgtagag tagaccccgg ggcatgacct ggggtctatt
      421 tacagagtgc cgcataatta ttttaaatgt agatttcgaa cttttgctta cagcagaaat
      481 caaaagttta atttcttctc agacaagcgg taaaggatat gcctatgacg ccggcaattg
      541 aaatgaaagg cattacgaag tattttgcat ccacacatgt ccgtgcaaat gagcaggtgg
      601 atcttacggt ggagcgcggc gaaatccatg cgctggtcgg cgagaacggc gccgggaaga
      661 cgacgctcat gaatattctc tacggcctga tcaagcccga ttcgggtgac atcctgatcg
      721 actcacaaaa cgtgcggatt gacaatccgg atgatgcaat caggcatggg atcgggatgg
      781 tccaccagca tttcaaattg gtttcgtcgt ttacggttgc tcagaacatc cttctcggta
      841 tggaacccca gcaagcaggc ttcctcgacc agcagaagga gaatgaactg gtggaccagc
      901 tggcgaaacg ctacaacctg ccggtgaatc ccgcggcgcg cattcgagac ctgccggtga
      961 gcatgcagca gcgggtggag atcttaaagg tgctggagcg caatgcgaag atcctggtgt
     1021 tggatgagcc caccgctgtg ctcacgccgc aggaggtaaa tgagctgatc acggtgatcc
     1081 gcaagctatc tgcgcagggc tataccatca tcttcatcac gcacaagctg atcgaggtga
     1141 tgtcggttgc ggatcgtgtc agtgtcatgc ggggtgggaa aatgatcggt acgaaaaagg
     1201 tatctgagac gtcgatcccg gaactggcgc gcatgatggt cggacgcgaa gtcctcctgc
     1261 gggtcaaaaa ggaggagcgg ccgcggggcg acgtggtgct ggacgttaa
//
LOCUS       DASTWE010000351         1086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_397205, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000351 DASTWE010000000
VERSION     DASTWE010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397205"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFT30_03745"
     CDS             <1..492
                     /locus_tag="VFT30_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091769.1"
                     /translation="LDLNRDRAGCMVKVMRQTRTIGWSMAITIAMSVSVCWSLSHADT
                     ITDGRSAIELATSNTNTMTSSTPIATEAIVKPMPSPAVPVENGLVLRTLPTVSKPISV
                     GGTMLVPYIGAGFGGGYATELDRSLNTAQSASSGSFNTGQKNLFGQQLIPNEVQLGVR
                     FPF"
     gene            604..753
                     /locus_tag="VFT30_03750"
     CDS             604..753
                     /locus_tag="VFT30_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091770.1"
                     /translation="MKEGYRLVLVDKDGVLVSEFQLTENALAQPEAFVAALRESIESV
                     EEEEP"
     gene            complement(822..>1086)
                     /locus_tag="VFT30_03755"
     CDS             complement(822..>1086)
                     /locus_tag="VFT30_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091771.1"
                     /translation="SAEKNNVEVAPKSEFSFLASKTTNWPSITVKVKDEKVPVTVTTI
                     TNGYLVKGKLPATVKGSYIRVEIFAKGPNDCDKADGWLLKVGK"
BASE COUNT          233 a          287 c          296 g          270 t
ORIGIN      
        1 cttgacttga atagggatcg agcgggttgt atggtaaagg tcatgcgtca gacgcgaacc
       61 atcggatggt ccatggcgat cactattgca atgagcgtga gtgtgtgctg gtcgctctcc
      121 catgcagata cgatcacaga cggacgttct gcgattgagc ttgccacgtc caataccaat
      181 acaatgacct cttcgactcc catcgccacg gaagcaatag tgaagccgat gccgtcccca
      241 gctgtgccgg ttgagaacgg attggtcctg cggacccttc cgactgtgtc gaaaccgatt
      301 tcagtgggag ggacgatgct cgtaccctac atcggagcag ggttcggtgg aggctatgct
      361 acggagcttg atcgttcgct caatacggcc caatctgctt cctctggctc tttcaatact
      421 ggacagaaga atttgttcgg gcaacagctg atcccaaatg aagtccagct gggtgtccgg
      481 ttccccttct agccggtctc catcccacct acgcaggaac catgattgtg atcccgcgag
      541 gttccactct gcagcaagcc ggttcctgtt cgttaccacg catctgaact acggaggaat
      601 gttgtgaaag agggctatcg ccttgtgctg gtcgataaag atggcgtgct ggtcagtgaa
      661 tttcaattga cggaaaacgc cctggctcag ccggaggcct ttgtggcagc gctgcgggaa
      721 tcgatcgagt cagtcgaaga ggaagagcct tgagggggat ttagattagc gccgcaacga
      781 cctgggcaga gaactcccta tccaggtcgt cttgctcaca gtcacttgcc tactttcagc
      841 agccagccat cggctttgtc acaatcgttg ggtcctttgg caaaaatttc gacccggatg
      901 tagctgcctt tcacggtagc gggcagttta cccttgacca ggtagccatt tgtgatcgtg
      961 gtcacagtca ccgggacctt ttcgtccttg accttcacgg taatacttgg ccagttggtg
     1021 gttttggatg caagaaatga aaattccgat tttggcgcca cctctacatt attcttctcc
     1081 gccgaa
//
LOCUS       DASTWE010000352         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1399304, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000352 DASTWE010000000
VERSION     DASTWE010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399304"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFT30_03760"
     CDS             <1..377
                     /locus_tag="VFT30_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU5091772.1"
                     /translation="LSLTDCYIGATVRCAPPGNNPAPDDFERCGRFLQDEVCLLTNHR
                     VVIALGKIAFDHYLKTCRTQGRAMPAPIPKFGHGVAYRFPWGVTLLGSYHPSQQNTFT
                     GKLTRSMFYAVFRRARKEIDRA"
     gene            complement(507..>1017)
                     /locus_tag="VFT30_03765"
     CDS             complement(507..>1017)
                     /locus_tag="VFT30_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEU5091773.1"
                     /translation="CKIVDGPVSAEVVSVEADAMVKEGKELAKIHKNIVVKCPLIPEG
                     LKATKRMAAEGIRVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSSGMELIRQIL
                     TIYKNYDYKTLVLVASVRHPQHVVEAALAGGHICTMPYSVFQALFKHPLTDAGLKKFL
                     DDWKAKGQQ"
BASE COUNT          206 a          295 c          269 g          247 t
ORIGIN      
        1 gcctgtcgtt gacggattgt tacatcggag caaccgtgcg atgtgccccg ccggggaaca
       61 atcccgcccc ggatgatttt gaacgctgcg gtcgattctt gcaagatgag gtttgtctcc
      121 tgacgaatca ccgcgtggtg attgctctgg ggaaaattgc cttcgatcat tacctcaaga
      181 cctgtcggac ccaggggcgt gcaatgccgg cgcccattcc caaattcgga catggcgtcg
      241 cctatcgctt cccatggggc gtgacattgc tcggctccta tcaccccagc caacaaaaca
      301 cgtttaccgg gaaattgact cgctcgatgt tctatgcggt gtttcggagg gctagaaaag
      361 aaatcgatcg ggcgtagctt aagagactgt caaaaggtac tcctcctggg caggccctct
      421 ttccactcat cttcgatttg agaggagcag tgtttggagt gggtgaacgt ccgccgaaca
      481 cggagcccac cctactcgtc aactttctac tgctgtcctt tcgccttcca gtcgtccaag
      541 aacttcttca gtccggcatc ggtcagtggg tgtttgaaga gagcttgaaa cacgctgtag
      601 ggcatcgtgc agatgtgacc gcccgccaat gctgcctcga caacgtgctg cgggtggcgg
      661 acgctcgcca ctaacaccag ggtcttgtag tcatagttct tatagatcgt cagaatctgt
      721 cgtatgagct ccatgccact tgagctgatg tcatcgagcc gtcctatgaa tggagacaca
      781 caccaggcac cggctttagc cgccaggagc gcctgtgtcg gagagaaaca gagagtcacg
      841 ttcactctga tgccttcggc ggccattcgc ttcgtagctt tcagaccttc cggaatcagt
      901 ggacatttga ctacgatgtt cttgtggatc ttggcgagct ccttaccttc cttcaccatg
      961 gcgtccgctt ccacgctcac gacctcggcg ctaactggcc cgtccacgat cttacac
//
LOCUS       DASTWE010000353         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1431663, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000353 DASTWE010000000
VERSION     DASTWE010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431663"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFT30_03770"
     CDS             <1..241
                     /locus_tag="VFT30_03770"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU5091774.1"
                     /translation="LALYWAQALAAQTADKRIAEKFKKIAKDLTDNEKAINQELLAAQ
                     GKPQDVGGYYHPDDAKAAKAMRPSATLNRIIDAIA"
     gene            315..1025
                     /locus_tag="VFT30_03775"
     CDS             315..1025
                     /locus_tag="VFT30_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5091775.1"
                     /translation="MNQEDTNVIDQPEVLKPRMVTIEIAGKKVDVPEGITVIKAMWYA
                     GQEVVRGVGCLGGFCGACATYYRTKDDPKVRTCLACQTAVQDGMSFTMMPPFPARKAT
                     YDIQKLQDPKQDLFNLYPEAPLCRNCNACTEACPQKIDVREGVWKAVFGDFKSVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPVGLDTRIKEIQDGRFNDEWN
                     KVLKMSDKELAEHCATVK"
BASE COUNT          306 a          344 c          363 g          242 t
ORIGIN      
        1 cctcgcgctt tattgggcgc aggccttggc cgcgcagacg gcggacaaaa ggatcgcgga
       61 gaagttcaag aagatcgcca aagacctgac cgacaacgag aaggcgatca atcaggagtt
      121 gttggcggcg cagggtaagc cgcaggacgt cggaggatac tatcatccgg acgatgcgaa
      181 ggctgcgaag gcgatgcgcc caagcgcgac gttgaatcgg atcatcgacg cgattgcata
      241 gattgcgtta ggttaggcgt gagggggggc tcttcacccc ctttacgcct ggcttcacgc
      301 atcacgaagg aacaatgaac caagaagata ccaacgtcat cgatcagcca gaggtcttga
      361 agccccgaat ggtgacgatc gagattgccg gcaagaaagt ggacgtgccg gagggaatca
      421 cggtcatcaa ggccatgtgg tacgccggtc aggaagtggt tcgcggcgtc ggttgcttag
      481 gtggcttttg cggcgcctgc gcgacgtact accgcacgaa agacgatccc aaagttcgca
      541 cctgtttggc ctgtcagacc gccgtgcagg acggcatgtc gttcacgatg atgccgccgt
      601 ttccggcgcg caaggccacc tacgacattc aaaagctcca agatcccaag caagatttgt
      661 tcaatctcta tcccgaagca ccgctctgtc ggaattgcaa tgcttgcacc gaggcctgtc
      721 cgcaaaagat cgacgtgcgc gaaggcgtct ggaaagccgt cttcggcgac ttcaagagcg
      781 tctccgagat gttcatggac tgcgtcatgt gcggcatgtg cacgccggtc tgcatcgcgg
      841 acatcgcgcc aaatcttgtg gcgctatatg tcagtcgcgc gcagggcgca cacttcaccg
      901 agaagccggt cgggctcgat acgcgcatca aggaaattca agatggtcgg ttcaacgacg
      961 agtggaacaa ggttctcaag atgagcgaca aggaactggc cgagcactgt gcaacggtga
     1021 agtaaagagt cattggtcct cgtcatcagt catcggaatg attctgaagt ttccactgac
     1081 taatgaccga tgactaatga cggaatgaca ctatggacat tcacgccctt caacagatcg
     1141 tacacaagac tcgagatgcc cgacgcaagc agaccatccc caagttttct tccgcagatc
     1201 gcgaccagct gattcacaag tatcacccgg actatcgcgc cagtgcctat cgtcc
//
LOCUS       DASTWE010000354         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_849844, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000354 DASTWE010000000
VERSION     DASTWE010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849844"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>1270
                     /locus_tag="VFT30_03780"
     CDS             87..>1270
                     /locus_tag="VFT30_03780"
                     /inference="COORDINATES: protein motif:HMM:NF037824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5091776.1"
                     /translation="MTNDKVYLKQNVLMQPLFNQWYAWSNLISPATAPMYIANLHLKV
                     MQSFIATPQVHMSAVKNPAMLGGPFMNYDSSRVPDIKALLDNTLKTQGHMIKFAEAVL
                     ACDKMLSAEADGTSLEPFYPRVPDILRGYVELVYDLNNHPSMRFIENLLYKSPYYSES
                     SQSVALSLVTDDSRPFVFSTPWLAGKGWLQLNMPFKDERLDYLFKMRRTPQPYEEVRD
                     LFKLGPEDEETFATFFTDEAPQPADRYTGDSVRIRYFGHACILFETKEVSILIDPVIS
                     YRYDNGIDRYTFSELPDKIDYVLITHNHQDHAMFETLIQLRHKIGCLVVPRSNSGSLM
                     DPSLKVLLQNIGFKNVREIDEMETIEVADGSILGLPFLGEHADLNIRTKIAHLVQLKG
                     KS"
BASE COUNT          312 a          336 c          327 g          295 t
ORIGIN      
        1 aggaagttag cccggcgcta ccgcgctcgg tactgaccgt atccaacaac attccttaaa
       61 ccggattcat tatcaggagt actagcatga ctaacgacaa ggtgtatctc aagcaaaacg
      121 ttctaatgca gccgcttttc aatcagtggt atgcctggtc caacctgatc tcacccgcga
      181 cggcgccgat gtacatcgcg aacctgcacc tgaaggtgat gcagtcgttc atcgcgacgc
      241 cgcaggtaca catgtccgcg gtgaagaatc cggcgatgct gggtggtcca ttcatgaatt
      301 acgacagcag tcgtgttccg gatatcaagg ccctgctcga caacacactc aagacgcagg
      361 gacacatgat caaattcgcc gaggctgtgc tcgcttgcga caagatgttg agcgccgaag
      421 cggacggtac ttcactcgag ccgttctatc cccgcgtgcc ggatatcctg cgaggctacg
      481 ttgagctggt ctacgacctg aacaatcatc cctcgatgag attcatcgag aacctcttgt
      541 acaagagtcc ttattactct gaatcgtctc agagcgtggc cctctcgctg gttacagacg
      601 atagccgccc gtttgtcttc agcacgccgt ggctggccgg taaaggatgg ttacagctca
      661 atatgccatt caaagatgag cgtcttgatt atctgttcaa gatgaggaga acgcctcagc
      721 cgtacgaaga ggttagagat ctcttcaagc tcgggccgga agatgaggag acattcgcga
      781 cgttctttac cgatgaagcg ccgcaacccg ccgatcgata caccggcgac tcagtgagaa
      841 ttcgttattt tggtcatgcg tgtattttgt ttgagacgaa agaagtttca attctcatcg
      901 atccggtgat cagttaccga tacgacaacg gtatcgatcg ctacacgttt tctgagctgc
      961 ctgacaagat cgactacgtg ttgattactc acaaccacca ggatcacgcg atgtttgaga
     1021 cgttgatcca gttgcgacac aagatcggat gtcttgttgt gccgcgcagc aatagcggca
     1081 gcctgatgga tccgtcgctc aaggtgctgc tgcaaaacat cgggttcaag aatgtcagag
     1141 agatcgacga gatggagacc atcgaagtcg cagacggatc gatccttgga ctgcctttcc
     1201 tcggtgaaca cgcagatctg aatatccgca ccaagatcgc ccacctggtt cagttaaagg
     1261 gcaaatcgtt
//
LOCUS       DASTWE010000355         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_268045, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000355 DASTWE010000000
VERSION     DASTWE010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268045"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1029)
                     /locus_tag="VFT30_03785"
     CDS             complement(<1..>1029)
                     /locus_tag="VFT30_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEU5091777.1"
                     /translation="HDHITLSGTASNAGNLSRALNMAEAYAPKKIVNAMQVGGVQQVM
                     LEVRVAEMNRELIKRLGVNFTGIGNDFFGLSVLANLTSIASLAHPAGQMATVTSGITQ
                     AIQGAFGFNAGGTSWTGFVDALKEENLVKVLAKPTLMALNGQEAAFLAGGEFPIPVPQ
                     AFGLVTIQFKKFGVGLVFTPNILDSKHISLNVAPEVSELDFSNALRTQGIIVPAITTR
                     RASTTIELGDGQSFAIGGLLRDNVRESVKKVPFLGEVPILGALFRSSSFQKNETELMI
                     IVTPHLVKPLDMAAQTLPTDYYVEPNDFEFYLMGFAEKGGYGGKAGQRSPAAEVLSNR
                     VAKGGAMEG"
BASE COUNT          223 a          304 c          257 g          245 t
ORIGIN      
        1 ccctccatcg cgccgccttt ggccacgcgg ttcgatagca cctccgcggc aggcgatcgt
       61 tgtcccgctt tcccgccgta tccgcctttt tcagcgaacc ccatcaggta gaactcgaag
      121 tcgttcggtt ctacatagta gtcggtcggt agcgtttgtg cggccatatc gagtggcttg
      181 accaaatgtg gcgtcacgat gatcatcaat tccgtttcgt tcttttggaa cgaactgctc
      241 cggaacaagg cgcctaagat cggaacttca ccgaggaagg gaaccttttt taccgattcc
      301 ctgacgttgt cacgcaacaa cccaccgatg gcgaagctct gcccatcccc caattcgatc
      361 gtcgttgaag ctctcctggt cgtgatggca gggactatga tcccttgggt tctcaacgca
      421 ttggaaaaat ccagttcgga gacttccggt gccacattca ggctgatgtg tttgctgtcc
      481 aagatattcg gcgtaaagac caacccgact ccgaacttct tgaattgtat ggtcaccaag
      541 ccaaacgcct ggggtacagg aatggggaat tcaccgccgg caaggaaagc agcctcctgc
      601 ccgttcaagg ccattagcgt cggcttcgcc aagactttca ccaaattttc ttccttgagc
      661 gcatcgacaa atcccgtcca agaagtgccc ccggcattga atccaaacgc gccttggatc
      721 gcctgggtaa tccctgaagt cactgtcgcc atttgaccgg ccggatgggc aagactggcg
      781 atactcgtta aattggcaag tacggacaga ccgaagaagt cgttgccgat tcctgtaaaa
      841 ttcactccga gtcgcttaat caactcccgg ttcatttcag ccacccggac ttccagcatc
      901 acttgttgaa cgccgccgac ctgcatggca ttcacgatct tcttaggcgc ataggcctcg
      961 gccatgttca acgcgcggct cagattgccg gcatttgatg cggtgccgga cagcgtgatg
     1021 tgatcatga
//
LOCUS       DASTWE010000356         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1173059, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000356 DASTWE010000000
VERSION     DASTWE010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173059"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1089)
                     /locus_tag="VFT30_03790"
     CDS             complement(<1..>1089)
                     /locus_tag="VFT30_03790"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEU5091778.1"
                     /translation="YLIGNDPKQWFTNVAHYARVKYEAVYPGIDLVYYGKQEQLEYDF
                     IVAPGADPSTIKQVFEGTEVVKIDEEGGLILRTRNGELRQHKPFIYQQTDDGHKRQIA
                     GRYVKTGKHQIGFEVDEYNPDLPLVIDPVLVYSSYLGGSGDDEVRAIATDGAGNAYVA
                     GLTTSAAFPTTAGAFQPIQPGGTDTFVTKLDPSGSVLVYSTYIGGSLGSEYTYALKVD
                     NQGHAYVTGSTFSIDFPTTPGVVDPVPGPAGDGFVTKLSPSGSSLIYSTFFGGSSSDI
                     PQSIAIDGAGQVSVTGYTFSLDFPTTLGAFDTTFNGVTSNEDAFVTKLNATASGFIYS
                     TFLGGSGRDLGTGLALDSSGNTYVTGYTT"
BASE COUNT          238 a          283 c          313 g          255 t
ORIGIN      
        1 agtcgtgtac ccggttacgt aagtattacc cgatgaatcg agtgcaaggc cggtgcccag
       61 gtcgcggccg ctgccgccga ggaaagtgga ataaatgaat cctgaggcgg tcgcgttcag
      121 cttcgtcacg aaagcatcct cgttactggt tacaccattg aaggtcgtgt cgaatgcgcc
      181 cagcgtagtc gggaaatcca gtgagaatgt gtaacccgtc acggagactt ggcccgctcc
      241 gtcaatggcg atggactggg gtatatcgga gctactacct ccgaagaagg ttgaatagat
      301 gagagatgaa cccgaaggac tgagtttcgt cacgaaacca tcgcctgctg gaccaggaac
      361 ggggtctacg acgccaggcg tcgtcgggaa gtcgattgaa aaggtactgc cggtcacata
      421 cgcatggcct tgattgtcga ccttgagcgc gtaggtgtac tcggacccaa ggctaccacc
      481 aatataagtc gagtaaacga gaactgagcc cgatgggtca agtttcgtta cgaaggtatc
      541 cgttccgccc ggttggatcg gttgaaacgc tccggctgtc gttgggaaag cggcagacgt
      601 cgtgagtccc gccacgtaag cgtttcctgc tccatccgta gcgatggccc ggacttcatc
      661 gtcaccgctc cctccgaggt aggatgagta caccagaacc gggtcgatca cgagcggcag
      721 atcagggttg tattcgtcca cttcaaagcc tatctggtgt tttccggttt tcacataacg
      781 tccggcgatt tgacgtttgt ggccgtcgtc agtttgttga tagatgaaag gcttgtgttg
      841 gcgtaactcc ccgttgcgag tgcgcaggat cagtccaccc tcttcatcta tcttaacgac
      901 ctccgttcct tcgaagactt gcttgatcgt tgatggatcg gcgcccggag cgacaatgaa
      961 gtcatactcg agttgttcct gttttccgta gtaaacgaga tcaatgccgg ggtagacagc
     1021 ctcgtacttc acccgagcgt agtgtgcgac gttcgtgaac cactgcttcg gatcgttgcc
     1081 gatcagata
//
LOCUS       DASTWE010000357         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1173095, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000357 DASTWE010000000
VERSION     DASTWE010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173095"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFT30_03795"
     CDS             <1..195
                     /locus_tag="VFT30_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091779.1"
                     /translation="VVQFRSHKTQPSHHLDGVHQRATPYSARREPQRLRVRAKIRLAP
                     SLPSGLARGEAHLGTSALDR"
     gene            295..996
                     /locus_tag="VFT30_03800"
     CDS             295..996
                     /locus_tag="VFT30_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5091780.1"
                     /translation="MGLPLLFAMVLHEYAHGWMAEKCGDSTAKREGRLTLNPLAHIDP
                     FGTVILPLICLMLPGSFLLGWAKPVPIDPRNMHQPRRDMALTAAAGPGMNLLLAVASA
                     LLLALLLTFEPTLAFRKTTDADAASGLFATMFLRPIAVMALYSVMINVFLALFNLLPI
                     PPLDGGRILTALLPPKPAMALARLEPYGMLILVGLIVFDKELGIIHTITGTFAKGLAG
                     TILSTAFSLRPGVAE"
     gene            993..1985
                     /gene="trpS"
                     /locus_tag="VFT30_03805"
     CDS             993..1985
                     /gene="trpS"
                     /locus_tag="VFT30_03805"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5091781.1"
                     /translation="MTAARRRVLSGMQPSGLMHLGNYLGALENWKALQEQYDCYFFVA
                     DWHALSTNYADTSRIRTFVREMLIDWLAGGIDPERATIFIQSRIPEHAILHLLLSMMT
                     PVSWLERNPTYKEKQEEIKEKDLTTYGFLGYPVLQASDILLYKPDFVPVGKDQLPHLE
                     LTRELARRFNDIYKTTVFPEPKEHLTKFPKVVGTDGRKMSKSYNNTINLSDTEPVVRQ
                     KLKTMVTDPARVRRTDPGNPDLCPLYEFHKIYSPQAIQDQVNQDCRTAAIGCIDCKKL
                     VADRMVEQLTPIWDARAKLTQHPSHVDEIVETGSKRAAAVASATLHEVNEAMKI"
     gene            2220..2528
                     /locus_tag="VFT30_03810"
     CDS             2220..2528
                     /locus_tag="VFT30_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091782.1"
                     /translation="MNQSSWKLVTLVFSLAASAPIASAADPPCDKYPSARQSHCAEIW
                     KELNKEDGPVIAQFGLDQQKRRDEGKINAQQHLAENMIFIKQSTEKRIERLKERIAKE
                     "
     gene            complement(2533..>3021)
                     /locus_tag="VFT30_03815"
     CDS             complement(2533..>3021)
                     /locus_tag="VFT30_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5091783.1"
                     /translation="LLALVALLWLLPVYFVFPEEPSGSHPDPVKIAEVTVRISDHGPV
                     VLLQAEGRTIPIFVDLTVALSIQSALNGEKLPRPMSHDLMHTILDAYGGKVTQTVITL
                     KGGTYYGALTVAFKDQVKVFDSRSSDSIALAVHFKAPILVGRDLLESAGKIPEKEKGA
                     EL"
BASE COUNT          712 a          900 c          751 g          658 t
ORIGIN      
        1 gtcgtccagt ttcgttctca caagacacag ccgtctcacc atctcgacgg cgttcaccaa
       61 cgtgccacgc cttattcggc acgacgtgaa cctcagaggc tccgcgtacg ggccaaaata
      121 cgcctcgccc cttccctccc ctccggcctt gcccgaggag aggcgcatct cggcacgtcg
      181 gcgttggaca ggtgagattg tcggcttttt tgaacaccct gcaggtattc ggactaggac
      241 gaaaactggc cattcatgaa ctccctttca cagatctttc acacgatctc atacatgggg
      301 ctccctctgt tgttcgcgat ggttctccat gaatatgcac atgggtggat ggcggagaaa
      361 tgcggcgatt cgaccgcaaa acgtgaaggg cgactcaccc tcaatccgtt ggcccatatc
      421 gatccgttcg gcaccgtcat cttgccgttg atctgtttga tgttgcccgg gagttttctg
      481 ttgggctggg ccaagccggt tccgatcgat cctcggaata tgcatcagcc tcggcgcgac
      541 atggcgctca cagccgccgc cgggcctggg atgaatctgc tgctcgccgt cgcgagtgcc
      601 ttgcttttgg cattgctcct cacgttcgaa ccaaccttgg cgttccgtaa gaccacagac
      661 gcagatgctg cttcaggcct gtttgcgacc atgtttttgc gtccaatcgc cgtcatggcg
      721 ctgtattccg tgatgatcaa cgtatttctc gcgctgttca acctgcttcc gatcccaccg
      781 ctcgacggcg gccgaatcct gacggcgctg ctgccgccga aaccggcgat ggcattagcg
      841 cgattagagc cttatggcat gctcatccta gtggggctca tcgtgttcga caaagaactg
      901 ggcatcatcc acacgatcac cggaactttc gccaaaggtc tggccgggac gatcttgtcg
      961 accgcgttca gcctccgtcc aggagtcgca gaatgaccgc agcacgcaga cgagtcctca
     1021 gcggcatgca gcccagcggc ctcatgcatc tcggcaacta tctcggtgcc ttagaaaact
     1081 ggaaagcatt gcaagagcag tatgactgct acttctttgt cgccgattgg cacgcgttgt
     1141 cgacgaatta tgccgatacc agccgcattc gtacgttcgt gcgtgagatg ttgatcgatt
     1201 ggctagccgg cggcatcgac cctgaacgtg ccacgatctt catccagtct cgtattcccg
     1261 aacatgctat tctccacctc ctgttgtcga tgatgactcc cgtttcatgg ctcgaacgca
     1321 acccgaccta taaagaaaag caagaagaga tcaaagagaa ggatctcacg acctacggct
     1381 ttctcggcta tccagtcctg caggcctcgg acatcctctt gtacaagccc gattttgtgc
     1441 ccgtgggaaa agatcaactg ccccatctgg agctgacaag agaacttgct cgacgattca
     1501 acgacatcta caaaacaact gtattcccag agccaaagga gcatctgact aagtttccca
     1561 aggtggtcgg tactgacggc cggaagatga gcaaaagtta caataatacg atcaacctgt
     1621 cggatactga gccggtcgtc cggcaaaagc tcaaaaccat ggtcaccgac cccgcgcgtg
     1681 tcagacggac cgatccaggc aatccggacc tctgccccct ctacgaattc cataaaatct
     1741 attctccaca ggcgattcaa gaccaggtca accaagactg ccgaaccgcc gccatcggct
     1801 gcatcgactg caagaaactc gtggcggacc gcatggtgga acaattaacg ccgatctggg
     1861 atgcccgcgc gaaattgact caacatccat cccacgtcga cgaaatcgtg gagaccggca
     1921 gcaaacgagc agccgcggtc gccagcgcga ccttgcatga agtcaacgag gccatgaaaa
     1981 tttagtccac attgttcatt taaccgaacc ccaactcctt ctcgtgaatt ccctctcacc
     2041 agttgaacgg caattaccct ctccactccc ccggcgataa catcgatcca tctcccctac
     2101 ctccccaatg cttaaggaag atctctgcgt ccttttcata gaccggaagg gtctccgcaa
     2161 cattgcgact tgtgatatgg tgattcgacg gatgatcggc cattctggtg gagagaccta
     2221 tgaatcaatc cagttggaag ttagtaactc tggtcttcag cttagccgcc tctgctccga
     2281 ttgcttccgc tgcagatccg ccctgtgaca agtatccctc cgccagacaa tcacattgtg
     2341 ccgagatctg gaaggagctc aacaaggaag acgggccggt catcgcgcag tttgggctcg
     2401 atcaacagaa acggcgcgat gaaggaaaga tcaatgcaca gcaacatctc gccgagaaca
     2461 tgatcttcat caaacaatca accgaaaagc gcatcgaacg actcaaggaa cgcatagcca
     2521 aagagtagcc tgctagagct ccgcgccttt ctctttttcg ggaattttcc cggctgattc
     2581 cagcaaatca cgcccaacaa gaatcggtgc tttgaaatgc acggccagtg cgatggagtc
     2641 ggacgatcgg ctgtcaaaca ccttgacctg atccttgaat gccacagtca acgctccata
     2701 gtatgtacca cctttcagcg tgatgaccgt ctgcgtcacc tttccaccgt aggcatccag
     2761 gatggtatgc atgaggtcat gcgacattgg ccgaggcagc ttttccccat tcagggcgct
     2821 ctgaatcgag agggccactg tgagatcgac aaaaatcgga attgtccggc cttcggcctg
     2881 cagtagcacc acaggaccat ggtcggaaat acggactgtt acttcggcaa tttttacggg
     2941 atcaggatga gagccggaag gttcttcggg gaaaacaaaa taaaccggca gcaaccagag
     3001 cagcgcaacg agtgccaaca g
//
LOCUS       DASTWE010000358         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_688501, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000358 DASTWE010000000
VERSION     DASTWE010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688501"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFT30_03820"
     CDS             <1..1050
                     /locus_tag="VFT30_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU5091784.1"
                     /translation="PAGVDVSDLILQKRAEHPNRLAGWIFRQQAKGTAFESFLRFLAS
                     TQRIWDRPFIRRLLERITTPIMNDMAPTARLPHDLVLPKLAQRHLREQYAHLIPSETD
                     SPPTRPVAYFHGCAANYFDDGVGDAVIEVLKKHGVEPALPPQRCSGTPIQTYGHIDLV
                     RDGARFNLRSLTPYETIVTGCASCTLMLKDYPTLFSEGTERQQAEELAKKVVHISEFV
                     ARSPQHPPMAKADRTTKRVTYHSSCHLRAAGVTKEPRQVLSSLPGVNFVEMQDADRCA
                     GGAGTYLIKDYDTSKKIFERKARAIALADADVVATSCPACMIQLKNGVGDTVQVKHVA
                     QLLQEAYRAAEHEQR"
     gene            1123..>1342
                     /locus_tag="VFT30_03825"
     CDS             1123..>1342
                     /locus_tag="VFT30_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5091785.1"
                     /translation="MVSIQIFGQTLRAMVEESELEVPVTGPTTVKQLIEAYPAQLGAL
                     LQYIKSREALITINKKIGSEDSPVRDGDV"
BASE COUNT          320 a          368 c          386 g          268 t
ORIGIN      
        1 ccggccggtg tggacgtatc cgacctgatt cttcagaagc gggcggagca tcccaaccgg
       61 ttggccggct ggatattcag acaacaagcg aagggaacag cctttgaatc gtttttgcgg
      121 ttcttggcga gtacacaaag aatctgggat cgcccattca taagacggct gctcgaacgg
      181 atcacgacac cgatcatgaa cgacatggcc ccaacggcac gcttgccgca cgaccttgtg
      241 ctgcccaagt tggcccaacg gcacttgcgc gaacagtatg cgcatttgat ccccagcgag
      301 acggactcac cgccgactcg gccggttgct tatttccatg gctgtgccgc aaactatttc
      361 gatgatggag tgggggacgc cgtaatcgaa gtgttgaaga aacacggggt cgagccggca
      421 ctgccgcctc aacgttgctc ggggacaccg attcaaacct atgggcacat cgatctggtt
      481 cgagacggtg cgcgattcaa ccttcgctcg ttgaccccat atgagacaat cgtgaccggt
      541 tgcgcgtcat gcacattgat gctgaaagac tatcccacac tgttttcaga agggaccgag
      601 cggcagcaag ccgaagaact ggccaagaaa gtagtgcata tttcggaatt cgtcgcccgc
      661 tcgccgcaac atcctcctat ggccaaagcc gacaggacga cgaaacgcgt cacctatcat
      721 tcatcctgcc atctgcgggc agccggggtg acgaaggagc cacggcaggt gctgtcatca
      781 ttgccagggg tcaatttcgt cgaaatgcag gatgccgatc gctgcgcggg aggcgcgggc
      841 acctacctca taaaagacta cgatacatcg aagaagatct ttgagcggaa agcccgcgcg
      901 atcgcactag ccgatgccga cgtggtggcg acgagttgtc cggcctgcat gatccagctc
      961 aagaatgggg tgggggacac ggtacaggtc aaacatgtcg cccagcttct gcaagaagct
     1021 tatcgggccg cagaacatga gcagcgctga gttcgtctcg ctgatcatgt atagtacggg
     1081 ctcactggaa gtgagatagt ctgttcactc gttgaggatt caatggtttc catacagatt
     1141 tttggccaaa ctttgcgcgc gatggtcgag gagagtgaac tcgaagtccc cgtgacaggt
     1201 ccgaccacgg tgaaacagct gattgaagca taccctgcac aactgggcgc cttgcttcaa
     1261 tacatcaaga gccgcgaagc gctcattacg atcaacaaaa agatcgggtc ggaagattcg
     1321 ccggttcgcg acggcgatgt cg
//
LOCUS       DASTWE010000359         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_236358, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000359 DASTWE010000000
VERSION     DASTWE010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236358"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFT30_03830"
     CDS             <1..402
                     /locus_tag="VFT30_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5091786.1"
                     /translation="PAEYADKHMPADWWGDAAKVEEGRKLFIGETNPDVNCASCHGKD
                     GKPVKAGARDFRNGERMKLYSDSVWFWRISEGVPNTKMKAWKSKLSDEDRWKLVLFER
                     NFGLSGKKWDDGKKEWADAGNMAVAQAGAAK"
     gene            455..>1221
                     /locus_tag="VFT30_03835"
     CDS             455..>1221
                     /locus_tag="VFT30_03835"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU5091787.1"
                     /translation="MKTWQRGLLACCALLALLGGAAFAQTPGAPPVEFPYTGNRTAVW
                     IVAQLHILFAAFILGAPIFVVISEWLGYRKHDPRYDRLAKEVMKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLVFAVIYPLLFIGETVLLYMYFYSWDAWKGEKKARH
                     IALGVLLNLVGTITLFVIDGPTSFMNTPVKAEGISPMEFLATASLWDKIYNYSWMPMN
                     LHRLVGNVTFGGFITGLVAAYMYMASKKEEDRAYYDW"
BASE COUNT          251 a          322 c          366 g          282 t
ORIGIN      
        1 cccgcggaat atgccgataa gcacatgccc gcagactggt ggggggatgc tgccaaagtg
       61 gaagagggcc gaaaactgtt catcggtgaa acgaaccccg acgtcaactg tgccagctgc
      121 catggcaagg atggcaagcc cgtcaaggcc ggcgcccgcg attttcgcaa cggtgagcgc
      181 atgaagctct attcggattc cgtgtggttc tggcggattt ccgaaggcgt acccaacacc
      241 aagatgaaag cctggaagag caagctctcg gacgaagacc gatggaagct ggtcctgttt
      301 gagcgcaatt tcggcttgtc cgggaaaaaa tgggacgacg gaaagaagga atgggcggac
      361 gcgggcaaca tggcggtggc ccaggctggc gcggctaagt aacgactcga ctgcattatc
      421 ctaatcggat tcgcggggga gggcggcgag acttatgaaa acatggcagc gcggtttgtt
      481 ggcttgctgt gcgcttctgg cgctgttggg gggagcggcc tttgcgcaga ctcctggcgc
      541 gccgccggtt gagttcccgt atacgggcaa ccggactgcg gtctggatcg ttgcccaact
      601 ccacatcttg tttgccgcat tcatcctcgg tgcgccgatc ttcgtcgtca tctccgaatg
      661 gctcggatat cggaagcatg atccgcgcta cgatcgtctg gccaaggagg tcatgaaggt
      721 cacggtcatt ctctacagca tgaccgccct gaccggcggt ttattcatct ttgtcttgct
      781 ggcgacctac ccgcagttca ccacctggct gatcaatcac ttcttcctgg tcttcgcggt
      841 catttatccg cttctcttca tcggagagac cgtccttctc tacatgtatt tctattcgtg
      901 ggatgcctgg aaaggagaga agaaggccag gcatattgcc cttggtgtcc ttctgaattt
      961 agtcggcacg attaccttgt tcgtgatcga cggccccacg tcgtttatga atactccggt
     1021 caaggcggaa ggtatctcgc ccatggagtt tctcgctacg gcgagccttt gggacaagat
     1081 ctataactac agttggatgc cgatgaattt gcaccgcctg gtgggcaacg tcacgttcgg
     1141 cggcttcatc accggtctcg tggcggccta tatgtacatg gcgtcgaaga aagaagagga
     1201 tcgggcatat tacgattgga t
//
LOCUS       DASTWE010000360         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_333327, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000360 DASTWE010000000
VERSION     DASTWE010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333327"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFT30_03840"
     CDS             <1..335
                     /locus_tag="VFT30_03840"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5091788.1"
                     /translation="RHHYRVPLAVQYPVMFSDAETIAEGKVMNLTVFGCAIECTGVAP
                     QRATLRVRLILPDQAQSLPVEEAEVRWVQGNRMGLQFHKVERAADFRLHGFVWDRMVE
                     RLRTITQE"
     gene            complement(326..1333)
                     /locus_tag="VFT30_03845"
     CDS             complement(326..1333)
                     /locus_tag="VFT30_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5091789.1"
                     /translation="MLRYILLALGLIVLSLLVWHIGPANIYEAATRLGPTALLALLIP
                     SVVMYSVEAYGWKVVLGRAAQRIPFWRLLTIRSAGEVVNMTTPTAYLGGEPLKVYLLK
                     KYDVSMAEGAASVVIAKTTMTIAEVFYILTGIALAFWILGAGSSAGQTITAALLSVGL
                     LVCSIAGFVFIQQRGLFASILSLVKKLGLRIRSLEAQEEHLRSIDKTIRNFYSHHQKA
                     FYASMGVYFLGWMAESMEVFGIIYLLGGSVSVLSALSIGALAVFIKGGSFFIPGSLGA
                     QDAGNLLLLQTFGYSDVTGITFALLRRFRELVWICIGLLCLATVGKGRIPQDQEVENH
                     S"
     gene            complement(1359..>1794)
                     /locus_tag="VFT30_03850"
     CDS             complement(1359..>1794)
                     /locus_tag="VFT30_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091790.1"
                     /translation="VARLTFTESPFGAWLDIVLDNIVHMAIFAGIAVGLYTTHIGQAD
                     DWVPLVLGVAAVFGNGLSFLLVEKAQRIKTVSGWKTPAHAAWADVMLKNVASRDFSAA
                     LLGFALFDQLYWFLLFAAAGSLLFAGAMVWVIRPSAVSSLRP"
BASE COUNT          458 a          509 c          484 g          343 t
ORIGIN      
        1 ttcggcacca ctatcgagtt cccctcgccg tgcagtaccc tgtgatgttt tccgatgcgg
       61 aaacgattgc ggaggggaag gtgatgaacc tcaccgtgtt cggctgcgcg atcgagtgta
      121 ccggtgtcgc gccgcagcgg gccaccctcc gcgtacggct gatcctgccg gatcaggcac
      181 agtcactccc ggttgaagag gcggaggtcc gatgggtcca agggaatcga atggggcttc
      241 agtttcacaa ggtggagcgg gcggcggatt ttcggctcca cggattcgtg tgggatcgga
      301 tggttgaacg gctccgaacg atcactcagg aatgattttc aacctcttga tcctgcggta
      361 tcctgccttt tccaaccgtc gccaaacaca acaacccgat gcaaatccac acgagctcac
      421 ggaaccgtcg caacagcgcg aaggtgattc ccgtcacgtc actgtagccg aaagtttgga
      481 gcaacagaag attgccggca tcttgggcac ccagactgcc cgggatgaag aaagatccgc
      541 ccttgataaa aactgcgagc gcccctatag agagggcgga caacacactc accgaaccac
      601 cgagtaaata gatgattcca aagacttcca tcgattcggc catccagccc aaaaagtaca
      661 cccccatcga cgcgtaaaag gccttttgat gatggctgta gaaattccga atcgtcttgt
      721 cgatcgagcg caggtgctct tcctgcgctt ccagtgaccg gattcgcaag cccaatttct
      781 tgaccaatga cagaatcgat gcaaagagtc cgcgctgctg gataaaaaca aaccccgcga
      841 ttgaacacac cagcagcccg acactcaaca gtgcagccgt gatcgtttgc cctgccgagc
      901 ttccggcacc cagaatccaa aaggcaaggg cgattccggt gagaatatag aatacttcag
      961 caatcgtcat ggtcgtcttc gcaatcacaa ctgaagcggc cccctccgcc atggaaacat
     1021 cgtacttttt gagcagatag actttcagcg gttcgccccc caggtaggca gtcggagtgg
     1081 tcatgttcac cacctcgccc gcgctccgaa tcgtcagcag ccgccaaaac ggaattcttt
     1141 gtgccgctcg ccccaatacc accttccatc cataggcttc aacggaatac atgacgacgg
     1201 atgggatcaa gagagcgagc aaagctgtcg gaccgagtcg tgtagcggcc tcatagatat
     1261 tggcggggcc gatatgccac acgagaaggc tgagaacgat gaggccaagg gccagtagga
     1321 tatagcgcaa cacgttagtc ggatggcgaa cgcggcattt atggccgaag agatgagact
     1381 gcagatgggc ggatgaccca gaccatggca ccggcaaaca gtaaggagcc agctgccgca
     1441 aagagtagaa accaatagag ttgatcgaac agagcaaagc ccagtaaggc tgctgaaaaa
     1501 tctcggctgg cgacattttt gagcatgacg tccgcccagg ctgcatgagc aggggttttc
     1561 cagccactca ctgtttttat cctttgcgcc ttttcgacaa gtaagaatga gaggccattg
     1621 ccaaacacgg cggcgacacc tagcacgaga ggaacccagt cgtccgcttg tccgatgtgt
     1681 gtcgtataga gtcccaccgc gatgcccgca aaaatcgcca tatgcacgat gttgtccaag
     1741 acaatatcga gccaggcacc aaaaggcgac tcggtaaagg tcaaccgcgc gact
//
LOCUS       DASTWE010000361         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1270380, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000361 DASTWE010000000
VERSION     DASTWE010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270380"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          243 a          254 c          265 g          257 t
ORIGIN      
        1 catcgtggtc gtcataatct ccatcttcat ctttcttgcc gtgaccgccg gggaggccag
       61 cacagcagga gccatgatcg ggcaaagaag gctcatgctc gtgacgatag ccgcagtagg
      121 caacgacgtc atcatcacct ccaagtgtgg ggttaggttg aactcagtcc tgagaatcag
      181 gagagctcat tgcttggatc acaataagtg gggagaaggt aattgaggcg gtttatcggc
      241 gtattacgtc cctgtaaagg atcggtaaaa agttcgagca atgcaggcga tgaaactctc
      301 gtggcttgca atacctgacg agaagcgcat acttcgcatg aactatcgtc tagcgtgaca
      361 aaagacgttt gtgccgcaac caactcatcc gaagcctaga aactgccagg taagaatcca
      421 tccgatccga tattgttcgc gatgtctgtg ggccaaatcg tcgataacaa gccagcaggg
      481 cgttcttctt tatcacgttg tttctgttaa catagcggct gtggttatga ccgtccctcc
      541 gatgatgccc tcgtttcgag tgcgatattc ctatgtaaaa cctaccgcga aaccgcacca
      601 cgcttgcggg tatcgtccgc cgccttagat ctccgtggag gctgtccgtt cttctcccgg
      661 gagcgagcga gtgtattagg gctaaagcca tagtttgagg ctgaaatgat ttggcggcga
      721 acatcctcaa tcgacggtgt ggatgaggtc accgacggat cttgtaggcc cagcacttcc
      781 cagcggaact tctttttact agtaagccct tttcttctgg gcgaggcctt aatcccccag
      841 gatggataca tagcgtttct ccaattcaaa acatgtgacc agagcaatgt gcggcaggtc
      901 agcgttttca gtaacggttt cttgcatgga tagcccaatc tgcatcgccg atccttacca
      961 atcggtctct ccaaatgcat tataaggagg tttggttacg ttgaagttac ggaacggta
//
LOCUS       DASTWE010000362         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1141185, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000362 DASTWE010000000
VERSION     DASTWE010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141185"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..496)
                     /locus_tag="VFT30_03855"
     CDS             complement(92..496)
                     /locus_tag="VFT30_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU5091791.1"
                     /translation="MSQVYVTRRYRFCAAHRLHTDQLSPEENWAAFGKCNNPNGHGHN
                     YVVLVTITSGATQESCDLNHLDRLVNERIVDRFDHHDLNSDPALAELTTTGENIVKLI
                     WDILEPQLPAGCLHKVGVIETRDNYFEYAGVA"
     gene            complement(603..923)
                     /gene="erpA"
                     /locus_tag="VFT30_03860"
     CDS             complement(603..923)
                     /gene="erpA"
                     /locus_tag="VFT30_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU5091792.1"
                     /translation="MVTITPVAEQKIKELMAEEKDVVGLRVYVRGGGCHGYQYGMAFE
                     SKMAEDDTVIEKGEVKLIMDSQSAPLLQGAEVDYVDSVQGSGFSIKNPQAKTTCGCGS
                     SFSA"
     gene            complement(1002..1337)
                     /locus_tag="VFT30_03865"
     CDS             complement(1002..1337)
                     /locus_tag="VFT30_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5091793.1"
                     /translation="MPRVTFLHSDGRSGEVEENISLLDAAKEVGFQLNHDCGGNASCT
                     TCRVEVQMGHEHLSEIDFDEQDLLDREALTEPWHRLACQARVLGDVVVRVPEAKWGNP
                     TTTTTEAWG"
     gene            complement(1346..>2457)
                     /locus_tag="VFT30_03870"
     CDS             complement(1346..>2457)
                     /locus_tag="VFT30_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5091794.1"
                     /translation="KWDKVLNHRVKERYFVCNAGEHEPGTFKDRHLLKTLPHQLIEGC
                     LIASYTVQAKASFIYVNHEYHEEQQNLKKALAQARERGLLGKNVLGSGFDIELEVFEG
                     HGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDFGLYGKPTLVNNVETLCNIPRILSK
                     GAGWFMQTGTEKCPGTMMFSLSGAINRPGVYEMPMGVTIRDLIEQCGGGVPNGRKIKA
                     VFPGGPAFSMVTADQLNLPMDFDSLKKAGTGLGSAGVIVVDDATCMVAKTLHFSNFFK
                     NESCGQCPPCRMGTINLASLMTKIESGQGTQKDLDSLLQLCGFVKGTGYCTLVTGASV
                     LVQSSLKLFRHEYEDHIRLQRCPYQETPVGIVAHS"
BASE COUNT          519 a          739 c          571 g          628 t
ORIGIN      
        1 atccgtcggc ttcccactcc cgctggtgtc ctcaactgat tctcgtcttt tctctctctt
       61 ctgttgcttc ctcactccgt ccctcgtcgt ttcaagcgac gcccgcatat tcgaagtagt
      121 tgtctctcgt ctcgatgaca cctaccttat gcagacaccc cgcaggcaat tggggctcca
      181 aaatgtccca gatcaatttg acgatgttct ccccggtggt tgtgagttct gccaacgccg
      241 gatcgctatt gaggtcatga tgatcgaacc gatcgacaat cctctcattg accagccggt
      301 cgagatggtt gagatcacaa gactcttgtg tcgctccgct cgtgatcgtg accagcacga
      361 cgtagttatg cccgtgcccg ttcggattat tacattttcc gaacgccgcc cagttctctt
      421 caggcgacag ctggtctgtg tgaagccggt gtgcggcgca aaaccgatag cgccttgtca
      481 catatacttg tgacattcga tcgtgtgtct ctggagtgga acaaaatcca ggaccgctca
      541 gatacgagcg gtcctggtgt gggagtcctt atcagatttc gtacggcgat ccgtatcggc
      601 gtttatgcgc tgaacgagct gccgcaaccg cacgtggtct tggcctgagg attcttgatc
      661 gaaaagccgg agccctgcac gctatcgaca tagtcgactt ccgcaccttg aagcaacggc
      721 gcgctctgag aatccataat gagcttcact tcaccctttt caatgacggt atcgtcttct
      781 gccattttgg attcaaaagc catcccgtac tgatatccat ggcacccgcc gccgcggacg
      841 tacacccgca atccaacgac gtctttttcc tctgccatca actccttgat tttctgctcc
      901 gccaccggcg tgattgtaac catcgtcttc ctcctctata gatcagtccc cgcactcacg
      961 ggcacaggat agctgttagt ctaacatctc tcgctcgaat atcaacccca tgcttctgtt
     1021 gtcgttgttg ttggattccc ccatttggct tccggcacgc gcacaacgac atcccccaag
     1081 acacgtgcct gacacgcaag gcgatgccat ggctccgtca atgcttctcg atccagcaag
     1141 tcttgctcat cgaaatcgat ctccgagagg tgctcatgcc ccatctggac ctccactcga
     1201 caggtcgtgc aggaggcatt tcctccacag tcgtggttta actggaaccc cacttctttt
     1261 gcggcatcga ggagcgaaat gttctcctcc acttccccgc ttcgcccgtc cgaatgaaga
     1321 aaagtcaccc gtggcatgac tcctcctaag aatgagcaac aatcccgacc ggtgtttcct
     1381 gatagggaca cctctgcagc cggatatgat cttcgtattc gtgccggaac agcttcaaac
     1441 tgctttgcac caatactgag gctccggtca cgagggtaca gtagccagtt cctttgacga
     1501 atccacatag ttgaagcaga ctgtccaggt ctttttgagt gccttgcccc gactcaattt
     1561 tggtcatcag cgaggctaaa ttgatggtcc ccattcggca tgggggacat tgcccacaac
     1621 tctcattctt gaaaaaattg gaaaagtgta gcgtcttggc caccatgcag gttgcatcat
     1681 cgacgacgat gacaccggcg gatcctagtc ccgtgccggc ctttttcaac gaatcgaagt
     1741 ccatcggaag attgagttga tccgccgtca ccattgaaaa cgccggacca cctggaaaga
     1801 ccgccttgat cttgcggccg ttgggcactc caccgccgca ttgctcaatc aggtcgcgaa
     1861 tcgtcacacc catcggcatc tcgtacacac caggacgatt gatcgctcca ctcagcgaaa
     1921 acatcatggt acctgggcat ttttccgtgc cggtttgcat gaaccaccca gcgcctttgg
     1981 agagaatcct cggtatattg cataacgtct caacgttatt gaccaaggtc ggtttgccat
     2041 agagaccgaa atcggtcgga tagaacggcg gtttctgcct cggcatcgcc ggacggccct
     2101 gcatcgactc gagcatcgcc gtctcttcgc cggctacata gcttccatgg ccttcaaaca
     2161 cttccaattc aatgtcgaag ccgcttccca acacattttt gccgagcagt ccccgttctc
     2221 ttgcttgagc caaggccttc ttaagattct gctgctcttc atggtactca tgattcacat
     2281 agatgaagga ggccttcgcc tgcaccgtgt aggacgcgat gagacagcct tcgatcaact
     2341 ggtgaggcaa cgttttgagt agatgacgat ctttaaacgt tcccggttca tgctctccag
     2401 cgttacagac gaaatagcgt tctttcaccc tgtggttgag aaccttgtcc catttga
//
LOCUS       DASTWE010000363         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1012186, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000363 DASTWE010000000
VERSION     DASTWE010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012186"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFT30_03875"
     CDS             complement(<1..231)
                     /locus_tag="VFT30_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091795.1"
                     /translation="MPRDERFVEIQERSRRRLRRWQVVLIGLVAVTIVGITTVAGIIW
                     HFSQDLPSLDLLQNYQPSLVTTVYSDDRQPIGQ"
     gene            421..2277
                     /gene="typA"
                     /locus_tag="VFT30_03880"
     CDS             421..2277
                     /gene="typA"
                     /locus_tag="VFT30_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5091796.1"
                     /translation="MSTVRAPHDRRSDIRNIAIIAHVDHGKTTLVDAVLRQTHVHRKI
                     DDMGERIMDSMDQERERGITIRAKNASVIYNGVKINIVDTPGHADFGGEVERTLRMVD
                     GVLILIDAKEGPMPQTTFVLRKALALGHKAIVVINKIDRPDAVIDDVVNRTFDLFVHL
                     GATDEQLDFPIVYTSAIKGIATLDVNKPGTDIIPLLDTVLEKIPAPAITVEAPLQILV
                     LALVQDSYKGKMGIGKIQSGSIARRQNVIVLGKNGTQIPGKVSDLAVYSGLERADTEQ
                     AAAGEIVAVAGLDEVSIGDTIADADRPVALPRVSIDEPTVQMTFSVNNSPFAGREGKF
                     LTSRHLRERLFKELETNVSLRVNETDSADRFLVAGRGELHLSVLIEQMRREGYELQVS
                     QPEVILHREGGAVMEPYEELTIQVPENYQGTVIEEIGKRRGEMRHMRLIHSDVGTSEM
                     HLEYHIPTRGIMGLKNLLLAKTRGTVIMHHVFSAYEPAEERDLIVAPHGSLVAYEDGS
                     STGYAIFMTQERGAMFIGPGVEVYRGMVVGQNSRDEDLDVNVCKEKHLSNMRASGSDE
                     ALVLTPPREMTLEFALEYIGDDELVEVTPQNLRLRKRLLNPDDRRKARKAGK"
     gene            2332..3084
                     /locus_tag="VFT30_03885"
     CDS             2332..3084
                     /locus_tag="VFT30_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091797.1"
                     /translation="MRIRKKAPKPSAKRPPLYFIGYRGTAPGTEEMKLLYEREYGAPL
                     AIRHEDGSAESWQATHGPWSAHVVIPLPMSHVAEIMKQLSWEHDLMGAVAPSIASPRD
                     MPDTVLLAARLARCLTLLTQGTAHDVITQAYVNPSDWQPRSLASFLLDDHVSIVHDDT
                     SQPDQVWSYSLGLSKFGLDEVEVFMAKGLSDSAAREVLIESAGELLRVGHSPKVGTAL
                     DLPQLSRTIHIRNYRTAAPAGRMLGFRELQTS"
     gene            3423..3893
                     /locus_tag="VFT30_03890"
     CDS             3423..3893
                     /locus_tag="VFT30_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5091798.1"
                     /translation="MSDVAPEIKVGDTAPDFNLKDQDQKDVKLSDYKGKKNVVLCFYP
                     LDWSPVCHGENKCLSDDFPKFQSANAELFGVSCDSFFSHKAWADSLDLKHRLLSDVHR
                     TTAKSYGLYFEPLNCSKRATVIVDKNGKVSYVKVQEIKTAREDKEILDALSKLN"
BASE COUNT          905 a         1090 c         1099 g          808 t
ORIGIN      
        1 ttggccgatg ggttgacgat catctgaata gacggtggtg accaaactgg gttgatagtt
       61 ttggaggagg tcgagcgatg gaagatcttg ggaaaagtgc cagatgattc ctgcaaccgt
      121 cgtgattcca acaatggtga cagccacgag accgatcaag acgacctgcc accggcgcag
      181 gcgacggcgt gaccgttcct gaatttcgac gaaccgttca tcacgtggca ttcacgtcac
      241 tcctgcggaa ggccgacctg cgaggctgga aaaccttaca atgcgttttc tcaaaactca
      301 agcttccccc gcgaatggca cctgtgaatg atcgcttgag agacggccgg cactcatgta
      361 tactttctcc gacgcctcgg tagcggggcg tctttttttt gtcattaagg atatcgtcat
      421 atgtccactg tacgtgcccc tcatgaccgc cggagcgata tccgtaacat cgccattatc
      481 gcccacgtcg atcacggcaa aacaaccttg gtcgatgcgg tgctccgcca aactcacgtc
      541 catcgcaaga tcgatgatat gggcgaacgc atcatggact cgatggatca ggaacgggag
      601 cgtggaatca cgatccgggc caaaaatgcc agcgtcatct acaacggagt gaaaatcaac
      661 atcgtcgata ctccaggcca tgccgatttc ggcggtgagg tggaacgcac gctccggatg
      721 gtggatggtg tattgatttt gatcgatgcg aaagaaggcc ccatgccgca aacgaccttc
      781 gtgttgcgga aagccttggc gctggggcac aaagccattg tcgtcatcaa caagattgac
      841 cgcccagacg ccgtcatcga cgacgtcgtc aaccgcacct tcgacctgtt cgtccatctg
      901 ggagccaccg acgaacaact cgattttccg atcgtctaca cgtcggcgat taaggggatc
      961 gccacgctgg atgtcaacaa gccgggtacc gacatcatac cgctactcga taccgtactg
     1021 gagaagattc ccgccccagc cattaccgtc gaggctccgc tccaaattct tgtgctggcc
     1081 cttgtgcaag attcctacaa aggcaagatg gggatcggca agatccagtc cggttcaatt
     1141 gcccggcggc agaatgtcat agtcctaggc aagaacggca cacagattcc cggaaaagtg
     1201 tcagacctcg cggtctattc gggccttgaa cgagccgata ccgaacaggc agccgccgga
     1261 gagatcgtcg cggtcgccgg gcttgatgag gtgagcatcg gcgataccat tgcggatgcc
     1321 gatcgtccgg tcgccctccc tcgggtgtcg atcgacgagc cgaccgttca aatgaccttc
     1381 tcagtgaata acagcccctt cgccggacgt gaaggcaagt tcctgacgtc acgccattta
     1441 cgcgagcgtc tgttcaagga gctggaaacc aacgtgtcgc tacgcgtcaa tgagaccgac
     1501 agcgccgacc gtttcctcgt ggctggccga ggcgaactcc atctctccgt attgatcgaa
     1561 cagatgcgga gggaagggta cgaactgcaa gtctcgcaac cggaagtgat tctccatcgt
     1621 gagggcggcg ccgtcatgga gccgtatgag gagttgacca tccaggtgcc ggaaaactat
     1681 cagggcacgg tcatcgaaga gatcggcaaa cgacgcggcg agatgcggca catgcggctc
     1741 attcactccg atgtcggcac cagtgagatg cacttggaat accacattcc gacccgaggc
     1801 atcatggggc tgaagaacct gctgctggcg aagacccgcg ggaccgtcat tatgcaccat
     1861 gtcttctcgg cctacgaacc ggcggaagag cgggacctga tcgtcgcccc tcatggctca
     1921 ctggtggcct acgaagacgg ctcgagtacg ggctatgcca tcttcatgac tcaggaacga
     1981 ggcgccatgt tcatcggccc cggcgtcgag gtctaccgcg gaatggtcgt cggccaaaac
     2041 agtcgagacg aagatctcga cgtgaatgtg tgcaaggaaa agcatctctc caacatgcgg
     2101 gcctccggat ccgatgaagc cttggtccta acccctccac gcgaaatgac gcttgaattt
     2161 gctttggaat atatcggaga cgatgagctg gtcgaggtaa cccctcaaaa ccttcgcctg
     2221 cggaagcggc tgctgaatcc agatgaccgc cgcaaagcca gaaaagcggg gaaatgagat
     2281 gtcgttcgct tgcgagatac gcttcgcgag gaacgagata cggttttcga gatgagaatc
     2341 cgcaaaaagg ctcccaagcc ttccgccaag cggcccccac tctatttcat cggctatcgc
     2401 ggtactgccc caggcactga agagatgaag ctcctctatg aacgggagta tggcgctcca
     2461 ttggccatcc gccatgaaga tggttccgcg gaatcctggc aagcgaccca tggtccttgg
     2521 tctgcccatg tggtgatacc cctgccaatg agccatgtcg ccgagattat gaaacagttg
     2581 tcctgggaac acgatctcat gggtgccgtc gcgccgtcga tcgcctcccc tcgagacatg
     2641 cctgataccg ttctgcttgc cgcgcgcctg gcccgttgtc tgactctcct gacacagggc
     2701 actgcccacg acgtgattac acaagcttac gtcaatccgt cggactggca gcctcgttca
     2761 ctcgcgagtt ttcttctgga tgaccatgtg tccatcgtcc atgacgacac ctcgcaaccc
     2821 gatcaggtat ggtcttattc actcggcctg agcaagttcg gcctggacga ggttgaagtg
     2881 tttatggcca aaggactatc cgacagcgcg gcaagggaag tgctgattga gtcggccggc
     2941 gaattactgc gcgtgggaca ttctccgaaa gtcggaacag cgctcgacct tccacagctg
     3001 agtcgcacga ttcacatcag gaactatcgg actgccgcac cagccggtcg gatgttggga
     3061 ttccgagagc ttcaaacctc gtaaggtccg cgacgcgaag aataacgagc gtcacgtttg
     3121 cggagggatg cgaggtccac gccatgccga ataaggcatg gtacgtctgt ggacgcccgc
     3181 gagatggtgc gcgggccgtg tcccgatgag cgcccagtgt cttagaaatc agttattttt
     3241 gtcggagcga tgcgagaatg ccgccgcagg cggacctttt caacaacccc taggcctacc
     3301 ggaccgttga catcccttta aaccacaggt tacaaagttt atcgttcatg gtggggtcct
     3361 gccaatccga gacctcagta cggcaatcac ccatacagat gttttaatga aggaggtttg
     3421 caatgagtga cgtggcaccg gaaattaagg tgggcgatac agcaccggac ttcaatctca
     3481 aggatcaaga ccagaaagat gtgaagttga gtgactataa aggcaagaaa aacgtcgtgc
     3541 tctgcttcta tcctctggat tggagcccgg tctgccatgg agagaacaag tgtttgtccg
     3601 atgacttccc caagtttcag tcggcgaatg ctgagctgtt cggcgtgagc tgcgacagct
     3661 tcttttccca caaggcctgg gcggattcct tggacctgaa gcatcgcctg ttatccgacg
     3721 tacatcggac gaccgcaaag tcctacggtc tttatttcga gccgttgaac tgctccaagc
     3781 gtgcgaccgt gatcgtcgat aagaacggga aggtctccta tgtgaaggta caggaaatca
     3841 agacggcgcg agaggataag gagatcctcg acgcgctttc gaagttgaac tagtggcaca
     3901 gg
//
LOCUS       DASTWE010000364         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1012187, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000364 DASTWE010000000
VERSION     DASTWE010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012187"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..762)
                     /locus_tag="VFT30_03895"
     CDS             complement(235..762)
                     /locus_tag="VFT30_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091799.1"
                     /translation="MAEATMTVSREVTDTMLQSLTRDATNKIAAAVEDVVSEFFMAGG
                     YGSLRMHMMSNEMIAKVFHSAARLMAIRAKAYADGKGAAVADLVEAHLFDLINRTMEK
                     RTEHIAAGKLPFQDAELLCADLRHNLKRLKDAAIRELRNCPSDIQIGESIRSRSKWLD
                     AHRTRQRVVVAIERQ"
BASE COUNT          324 a          425 c          408 g          343 t
ORIGIN      
        1 ccgtcaaacg agggcaccaa gtgttagatt caatccacta ggatgcgaat gcgctcgccc
       61 tgcatctcca acgtgtcgcc gtcggtaacc gtgatgcgcg atagttttgg cgcgggtggt
      121 ggaactggcg agaagcaaga acagggcggc gacaggcagg cgcacgagaa atagattggc
      181 aaacgtgatc tcaatccaat ggattcgtag tattcgcacc tgttctcgag gtggtcattg
      241 ccgctcaatg gccacaacca cacgctgccg cgttctgtgg gcatcaagcc acttcgatcg
      301 cgaccttatg ctctcgccaa tctgaatgtc ggaaggacaa ttgcgaagtt ctctgatcgc
      361 ggcatccttc aatcgcttca gattgtgcct cagatcagcg cagagcagct ctgcgtcctg
      421 gaacggaagc tttcctgccg cgatgtgctc ggtgcgtttc tccatcgtac gattgatcag
      481 atcgaaaaga tgtgcttcga ccagatcggc cactgccgca ccctttccat cggcgtaggc
      541 cttggctcgg atagccatca ggcgcgcggc agaatggaaa acttttgcga tcatctcatt
      601 gctcatcatg tgcatgcgca atgaaccata gccgcccgcc atgaagaact cgcttacaac
      661 gtcttcaacg gccgcagcga tcttgttggt cgcatcccgt gtaagtgatt gcagcatggt
      721 gtcggtaact tcgcggctca ccgtcatggt tgcctccgcc attcaggacg atgctccctt
      781 gcgcaccgat catggccagc cgcttgccgg cacgatgttt cgatgtgctg gtggggaatg
      841 attcggacgc aagtacttga taaacaagac aacgaggcga acggagctgg gcatcggcta
      901 ccgaaggtct cgccatcgaa gaccgatctc aagagatcag gcacgacgca aggcgttggc
      961 aacaaatgta gcaactgaag gaatcgttcc gacgcgagtg agattatttt ggaacaagtg
     1021 tctcgtgata cgacgtccgc ggtgccgtta cttcggtgtc aatccgacag ccgcccgccg
     1081 gctttcagag acgcccattc accgatgcca tgcaagctcc tgccggaagt agcgctgccg
     1141 atattcaggc tcctttgagg atcgctgctg gcctggctgc cgtccttggc gtcgattcaa
     1201 cgccaaccct tcgattacga ccgcagatag atagccaaag cgatcggcgc gcaactactg
     1261 caaccgtcta aaggaccatt gtccccgaac aggaatcgct ctttggcgta agaacgattg
     1321 cttcgacaac tcttcgaaca taacggtggt gatgttgctg ctgctgctgg gatctcgcat
     1381 aggcgatcac ttttctgtcg gattctcgtg gcgcccctcc gtctacagtg acctttaaat
     1441 ccgtcctcaa atgggtagat cgtttggctg actgcccttg ctccgtgatc cagtcggttg
//
LOCUS       DASTWE010000365         1311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_850385, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000365 DASTWE010000000
VERSION     DASTWE010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850385"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFT30_03900"
     CDS             <1..482
                     /locus_tag="VFT30_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091800.1"
                     /translation="KHRVEQNRAERVQEAEKAERMVLEEVTTMLDWMKSLEVTPTIVA
                     LRNRVDDMKRAEVDKVLARLPHLSPQDRELVEGLASSIVNKLIHRTMVTLKAEVNSSS
                     GPAFVEAARRFFSLDRQSPSVQQIETYTGSPRYQTESSTEPGVEDPAPETSIHRRAR"
     gene            509..>1311
                     /gene="hemC"
                     /locus_tag="VFT30_03905"
     CDS             509..>1311
                     /gene="hemC"
                     /locus_tag="VFT30_03905"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU5091801.1"
                     /translation="MSIQNSRRSTLVLGTRGSKLALCQSEWFQSKVQEIVPEVQVILK
                     KIQTSGDKIVDVPLAKIGGKGLFVKEIEDALLAGEIDFAVHSMKDVPAQLPDGLEILC
                     VPPREDARDALISREGYSFQDLPFGANIGTASLRRQAQLLQARHDLKIAMLRGNLDTR
                     LRKLKEGQFDAIVLAAAGLHRLGWSQTITEYLPPLLSLPAIGQGALGIEGRSNDTFVR
                     SVLSRLNHQATHTTVAAERAFLCRLEGGCQVPIAAHATLSGERLVLDGLV"
BASE COUNT          311 a          337 c          380 g          283 t
ORIGIN      
        1 tgaaacatcg agtcgaacag aatcgagccg agcgagtgca ggaggcggag aaggccgaga
       61 ggatggttct ggaagaagtg accaccatgc tggattggat gaaatccttg gaggttactc
      121 cgacgatcgt ggcgctcagg aaccgcgtcg atgacatgaa gcgggcggaa gtcgacaaag
      181 tgctcgcgcg attgccgcac ctgtctcctc aagaccgcga attggttgag ggcctcgcct
      241 cctcgattgt aaacaaattg attcatcgca cgatggtcac tctcaaggcc gaagtcaact
      301 cttcgagcgg tcctgcgttc gttgaagcgg cacgtcgttt tttttctctc gaccgacaat
      361 ctccgtctgt tcaacagatt gagacctata cgggttcccc gcgttatcag accgagagct
      421 ccacggagcc gggtgtcgag gatccggctc cagaaacatc aatccacaga cgagcccgtt
      481 aaaagcgggt aacgaaaaga gtcagaacgt gtcgatacag aatagccgac gctcaacctt
      541 ggttcttggg acgagaggga gcaaattggc actctgtcaa agtgaatggt ttcaatccaa
      601 ggttcaggaa atcgtgccgg aagtccaggt tatactcaag aaaattcaga cgtccggcga
      661 caagattgtc gacgtgccgt tggcaaaaat cggggggaaa ggtttgttcg tcaaggaaat
      721 cgaggacgca ttgctcgccg gcgagatcga cttcgcggtt cacagcatga aggatgttcc
      781 agcgcaattg cccgatggac tcgaaattct ctgtgttccc ccacgtgagg atgcccgtga
      841 tgccttaatc agtcgtgaag ggtactcgtt tcaggatctt ccattcggag ccaacattgg
      901 gactgcaagt cttcggcgac aggcgcaact gttacaggcc aggcatgact taaaaatcgc
      961 gatgctgcgc ggcaacttgg atacgcgact gagaaaactc aaagagggac agttcgatgc
     1021 catcgtcttg gcagcggctg gtttgcaccg gttggggtgg tcacagacca tcacggaata
     1081 tcttcccccc ctcctcagtc tgcctgcgat cggacaggga gctcttggca tcgaaggtcg
     1141 gagcaacgac acattcgtac gttccgtctt atcccgactc aatcatcagg caacccatac
     1201 gactgtggca gcagagcggg cttttttgtg ccggctagag gggggctgtc aggtgcccat
     1261 cgcagctcac gcaaccctgt ccggtgagcg gctggtattg gatggtcttg t
//
LOCUS       DASTWE010000366         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1432281, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000366 DASTWE010000000
VERSION     DASTWE010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432281"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFT30_03910"
     CDS             complement(<1..497)
                     /locus_tag="VFT30_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091802.1"
                     /translation="MTRPTATMSPVVQQVVLCVLAAVLLSEAGCVSRRTYESVKAEML
                     EQTQALESVREDVRELDREIAGLQASNRREDAAVFELRATIQREEEQLPIMRQRAEDT
                     LASLKTQVATLMNQSWHLARKIVDIRQESVSLQTKVAQYKEEMEQALSPVMAASERDK
                     PAITQA"
     gene            675..1268
                     /locus_tag="VFT30_03915"
     CDS             675..1268
                     /locus_tag="VFT30_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091803.1"
                     /translation="MSSDATTSTRLPPECEPALLERFLKAEAMALWTVRSARLQDVPL
                     NVYAFLRKHEEDEQQHLAQFETIIGRQPRERERLPSVPRQWPALAVQLYGYESLGLEF
                     ATLLVTLRPDLSSIRADELVHVDFFEHEIHRLLGGEAAAAEQARVSALAWWRKLPRTI
                     DRYLDAAVFDPFRLVLTSKILSAIGRRFTTTGLLKDQ"
BASE COUNT          264 a          378 c          358 g          298 t
ORIGIN      
        1 gcctgggtta tggcaggctt gtccctctcc gacgccgcca tcacgggcga gagagcctgc
       61 tccatttcct ccttgtactg agcgaccttg gtttgcaagg agacgctctc ctgccgaatg
      121 tccactatct tgcgcgccag atgccagctc tgattcatca aggttgccac ctgcgttttc
      181 agcgacgcga gagtgtcctc cgctcgctgt ctcatgatcg gcaactgttc ttcttcccgc
      241 tgaatggtgg cccgcagttc gaacaccgcg gcatcttcac gtcggttcga agcctgcaat
      301 cccgcgatct cccgatcaag ctccctgacg tcctcgcgta ccgattccaa ggcttgagtc
      361 tgctccaaca tctccgcctt gacactttcg tacgtacgcc gactgacgca gccggcctcc
      421 gagagcaaga ccgcggcgag cacacagaga acaacctgtt gaacgaccgg agacattgtg
      481 gcggtaggac gtgtcatagg tgttgcctca tggttaacca aaccgaaacc cttgggcccg
      541 ggttagcagt cggtccggtg agtagctagg gcgtaagatg tacacagatg atgcatgtca
      601 gtcaaccctt ctcacccttc ccttgtcaag agtgcttgga taacccatag gagtttgcgt
      661 actatgagtc catcatgtca tctgatgcga cgacgtccac tcggctcccg ccggaatgcg
      721 agcccgccct attggaacgg tttctcaaag ccgaggccat ggcgctttgg acagtacggt
      781 cggcgcgttt acaagacgtt cctctaaatg tttatgcctt tcttcgaaaa catgaggaag
      841 atgaacagca gcatctggca caatttgaga ccataatcgg ccgtcagcct cgcgaacggg
      901 aacggctgcc gtccgtgcca agacaatggc ctgcgctggc cgttcagctc tatgggtatg
      961 agtcactggg acttgaattc gcgacgttac tggtcacatt acgaccggac ttgagctcga
     1021 tccgcgcgga cgaacttgtc catgtggatt tttttgagca tgagattcac cgcctcctcg
     1081 gaggcgaggc tgcggctgcc gaacaagccc gggtgtccgc gcttgcgtgg tggcggaaat
     1141 taccgcgaac gatcgatcgc tatctcgacg cggcggtgtt tgatccgttc cgccttgtgt
     1201 tgacaagcaa gattctgtct gcgatcggac ggcgattcac cacaacggga ttgttaaagg
     1261 accaatgaca tcaatcaggc ttgggtgggg agaaggcc
//
LOCUS       DASTWE010000367         1153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_624032, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000367 DASTWE010000000
VERSION     DASTWE010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624032"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFT30_03920"
     CDS             <1..235
                     /locus_tag="VFT30_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU5091804.1"
                     /translation="QQQRARDKASLSEDEQRRIMDSIRAQYEREGSPYFSTARLWDDG
                     IIDPVDTRKILGLCLDVTMTTPVRSSRFPVFRM"
     gene            243..1043
                     /locus_tag="VFT30_03925"
     CDS             243..1043
                     /locus_tag="VFT30_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5091805.1"
                     /translation="MRPTRSILVESSKGYARVTLNRPDRRNAFDIRMVEELYETFQAL
                     GQDHSVRVIVLAGSGPTFCAGADLAWMGADRIVSAPDAQQDAERLLAMFRAIDDCSCP
                     VIGCIQGAVYGGGIGLIAVCDIAVAAANATFAFSEVRLGLVPAVIAPFVLAKAGDSFA
                     RRFCLTGEPFSASTAQDNGLIHDVVEPAALEAHVASLTEQITQLAPQAVRDTKALFHR
                     IYHLSHEERWKACVEGNVRARLSYEAQEGIRAFRERRNPNWVMPTGGE"
BASE COUNT          240 a          296 c          356 g          261 t
ORIGIN      
        1 acaacagcag agagctcgtg acaaagcgtc gttgtcggaa gacgaacagc gaaggattat
       61 ggactctata cgagcccagt atgagcggga gggcagtccc tatttcagca cggctcggct
      121 ctgggatgat gggatcatcg atcctgtgga tacacggaaa attcttggtt tgtgccttga
      181 cgtcacaatg acgacaccgg tccgctcgtc gcgcttccct gtttttcgca tgtgaggagc
      241 ccatgagacc aactaggtcg atcctcgttg agtcgagtaa agggtacgca cgtgtgacct
      301 taaatcggcc cgatcgacgg aacgcgttcg atatccgcat ggtggaggaa ctatacgaga
      361 cctttcaggc attgggacag gatcattcgg tgcgcgtcat cgtcttggcc ggtagcggcc
      421 cgacattttg cgccggggca gaccttgcct ggatgggggc tgaccgaatc gtgtctgcac
      481 cggacgcgca gcaggatgcc gaacgcttgc tcgcgatgtt tcgggccatc gatgactgtt
      541 cctgtcccgt gatcggatgc attcagggag ctgtatatgg cggaggaatc gggttgattg
      601 cggtgtgcga catcgccgta gccgctgcga atgcgacatt cgctttcagc gaggtccgct
      661 tggggttggt cccggctgtg atcgcgccct ttgttcttgc aaaggccggt gactcttttg
      721 cgcgacgatt ctgtctaacc ggtgaaccgt tttcagcatc gaccgctcaa gacaacggtc
      781 tgatccatga tgttgttgaa ccggctgcgc tagaggccca tgtcgcatca ttgactgaac
      841 agattaccca actcgctccg caggccgtac gggacacgaa agccctgttt caccgaattt
      901 accatctgtc ccatgaggag cgctggaagg cttgtgttga gggaaacgtt cgggctcgcc
      961 tttcatatga agcgcaggaa gggattcgtg cctttcgcga acgtcgaaat ccgaactggg
     1021 ttatgccaac gggcggggag taaaggggct gttgtggagc gccgcatgac gcaagacagc
     1081 accggaccaa aatcaaatgc gtctgtgatc cggatcgtcg aggtcggtcc tcgagatggg
     1141 ctgcagaatg aat
//
LOCUS       DASTWE010000368         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1561469, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000368 DASTWE010000000
VERSION     DASTWE010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561469"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFT30_03930"
     CDS             complement(<1..728)
                     /locus_tag="VFT30_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5091806.1"
                     /translation="MKRSRRSFLQDAIALSATLTASRITSATEQSERGSLKEPADIRR
                     VGYKRIATEEAWGPSELLATYQKMAASKAIDDRGFVALWARLGTNAQLLSRLADIGEG
                     RIRDMDATGIEMQVLSLTSPGVQVFDAASANKIASDTNDQVAEAIRKYPTRFAGLAAA
                     APQDPKAAAKEIERGVRKLGLKGVIINSHTRGEFLDDPKFWDIFEAAEGLNVPIYLHP
                     QAPPQAMIMPFVERGLEGAIWGFGA"
BASE COUNT          234 a          308 c          273 g          241 t
ORIGIN      
        1 gccccgaatc cccaaatagc cccctccaaa cctcgctcaa cgaaaggcat gatcatcgcc
       61 tgtggaggtg cctgcgggtg aagatagata gggacattca acccctcggc ggcttcgaag
      121 atgtcccaga actttggatc gtccaaaaac tcgccgcgcg tatgtgagtt gatgattaca
      181 cctttgagcc cgagcttgcg aaccccacgc tcgatttctt tcgccgccgc tttcggatcc
      241 tgaggtgccg ccgccgccag tccagcgaag cgtgtcggat acttacgtat cgcctcggcc
      301 acttgatcgt tggtatcaga tgcgatcttg ttggccgaag cagcgtcgaa gacttgaacg
      361 ccaggcgagg tgagcgagag aacctgcatt tcgatgccag tagcatccat atcgcgaata
      421 cgtccctcac cgatgtcggc cagcctcgac aagagttgtg cgtttgtacc aagcctggcc
      481 cacagggcga caaaaccgcg atcgtcgatc gccttactcg ccgccatctt ttgataggtg
      541 gccaagagtt cggagggccc ccaggcttct tcggtcgcaa ttcgtttgta accgaccctc
      601 cggatgtcgg caggttcctt cagcgaacca cgttcagact gttctgtcgc agaggtgata
      661 cgactagcag tcaaggtcgc ggaaagggca atggcgtcct gcaaaaatga tcttctgctt
      721 cgtttcatat gatctcccga actggatcac atcaatatcc tctcaagaac atgcgatttc
      781 agattccttc cggattcata ccacggctaa gccgccgaac gctgcgcttg agcggctcgc
      841 ccacgcaact tgaatagagg cccgttcatg gcgagtccgc tccaagcgcc tgttgaattg
      901 cgcgttagga ttaatcacga cggctcggtt taagaattct tccaacgcac ttcctgacta
      961 cgtgattagg tgtggctgag tcgaaatctg gattgtatcg agccggctta cccagtggca
     1021 cgagccataa ggacacttct aatgaatcgc gatacc
//
LOCUS       DASTWE010000369         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1302969, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000369 DASTWE010000000
VERSION     DASTWE010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302969"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFT30_03935"
     CDS             complement(<1..775)
                     /locus_tag="VFT30_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5091807.1"
                     /translation="MTNGAKLRAALKRPGALKIVGAHDALSARLIERAGFDGVWASGF
                     AISASLKCIPDASFITASEQLDVERNIVEAVSIPVIADCDTGYGNALNVMRTVNDRER
                     AGVAAICIEDNVYPKRCSFYAGVRRELIPIEEHCGKIRAAKAAQIFPEFMVIARTEAL
                     IAGWGQEEALKRAEAYADAGADAILIHSKSKKFDELKAVYQLWSGRVPLVVVPTIFDQ
                     TTAAEMEEAGAKIIIYANQPVRAAIRAMTDALDLIKKDTR"
     gene            complement(1253..1783)
                     /locus_tag="VFT30_03940"
     CDS             complement(1253..1783)
                     /locus_tag="VFT30_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091808.1"
                     /translation="MKNSIQPSSHIWPLILILAIVIVASSVWAVPMTNDPKGFHDIPW
                     GTSLSSRQDLEPMQTDPHIAEYLHKTESSSFAGTQMTSILYVSVDDRFARVIIRYQGD
                     DVHRLVLDFLESRFGRLDRVPGQMTRGLTQQYNWRGPDTEINLTYQAGTERGYIFFDS
                     RTLAPRFNDYITGPSE"
BASE COUNT          430 a          524 c          535 g          485 t
ORIGIN      
        1 ggcgcgtgtc ctttttgatg agatccagcg cgtccgtcat tgcacgaatg gccgcccgta
       61 ccggttggtt ggcatagatg ataatctttg cgccggcttc ttccatttcc gccgccgtgg
      121 tctggtcaaa aatggttggg accacgacca gagggacgcg cccggaccac aattggtata
      181 cggccttcag ctcgtcaaac tttttcgatt tcgagtggat caggattgcg tcggcacccg
      241 cgtctgcata ggcttcagcc cgtttcagtg cttcttcctg tccccaaccg gcgatgaggg
      301 cctcggttct ggcgatgacc atgaattctg gaaaaatctg agccgctttc gccgctcgaa
      361 ttttgccgca gtgctcttca atgggaatca actctcgtcg aacacccgca taaaaactgc
      421 agcgctttgg atagacgttg tcttcaatgc aaatagcggc cacgccggct cgctcgcggt
      481 cgttgacggt ccgcatgaca ttcagcgcgt tgccatatcc ggtgtcgcaa tcagcgatca
      541 caggaatgct cacggcctca acgatattcc gctcgacgtc gagctgctcg cttgcggtga
      601 taaaactggc gtctgggata cacttcagcg aggctgaaat agcgaagccg ctcgcccaga
      661 caccgtcgaa accggcgcgt tcgattagac gggcgctgag tgcatcatga gccccgacga
      721 ttttcaaggc acccggcctt tttaaggcag ctcggagttt tgcaccgttg gtcatggggc
      781 aacatgatgc gaaagcgtac ggcggttgtc aatgaaaact tttgtcccgc ctgggggaag
      841 ggcaaaggcg atggcagagg tctcatcagc cagttcccgg caccgtacga tttcccaaaa
      901 atatgatgcc gcgatcgagc ccggtttcaa atcgtaacgg tacttccgta tgaaatccgt
      961 gcaggcatag gctttcaccg ggccgactgc gatttgtccc ggtattcgat cgagcggacc
     1021 atacttcgtt gaagatacat tgggattccc tcacgggctt ctttgccgct gtcgtgaaca
     1081 gtcactcggc tcaacttctt ctcaaacgtc gtaaaccgta tcgaatcgcc cggggtcgtg
     1141 tctagctagg ggcgtccatc cgttcttaaa tctgtaagcg gtcggcatcc tcaatttttt
     1201 gcgaagcgtt ctgtcggtca tggatttccc agaaagaaac gtattcatcg aactattctg
     1261 agggaccggt gatgtagtcg ttgaaccgtg gcgcaagcgt ccgactatcg aagaagatat
     1321 agccacgttc tgtaccggct tgatatgtca gattgatttc ggtgtcgggc ccgcgccaat
     1381 tgtattgctg ggtgagtcct cgcgtcattt gtccgggaac gcggtccagc cgaccgaacc
     1441 ggctttccaa gaagtccagt accagccgat ggacgtcgtc cccttgatat cgaatgatga
     1501 cacgcgcgaa tcgatcgtcc accgagacgt agagaatgct ggtcatctgc gttccggcaa
     1561 atgatgatga ctctgtcttg tggagatact cggcgatatg cggatctgtc tgcatcggtt
     1621 ccaagtcctg acgcgaagat aaggaggttc cccaaggaat atcgtgaaat cctttcggat
     1681 cgtttgtcat gggaacagcc catactgagc tggccacgat cacgatcgcg agtatcagga
     1741 tcaatggcca aatatgggat gatggttgta tagaattttt catctctgca aacgaatcgg
     1801 accacagctg tttcggcact aacgctaaca tgcgagtgaa gtcaactcta ctgcggatct
     1861 tcttgagaac cccgctggcc atattttata atgcgccggc tttgggtggg cgtgtatcaa
     1921 aattccagga gaactcccag tgattgaaag cgactggttt gtgcaagcca tgca
//
LOCUS       DASTWE010000370         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_689027, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000370 DASTWE010000000
VERSION     DASTWE010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689027"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1050)
                     /locus_tag="VFT30_03945"
     CDS             complement(40..1050)
                     /locus_tag="VFT30_03945"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEU5091809.1"
                     /translation="MKTPFLSGNSMAWRSTVMLGLLVGLLAFPIGCAAERDGSLRTVV
                     DEEWQPWQILETQTGRVLPFSELITNLERYDIVYLGEEHHNPYHIQAALKVLGQLVTD
                     GIEPTIGMEMFGWDGQPALDGYVATPQAVTNEFLEEVRWKQNWGGAFEDYAPLVTFAR
                     DRRLSVRAMNPPKPLIRRVVKLGLDQARQEPEWAPWGILQEDIVDDPAYRERIVDQLR
                     RCHGGTEEHFRTMYEASMVRDEGMARTLAIRQEEFRRENGGSHRMIVSYTGGGHIQYN
                     LPVPKRVARRLGGDIKQITIYMTSFEPGKTADVRALMQEPIADYIWLTPMGKSSSTKP
                     CR"
BASE COUNT          236 a          313 c          261 g          263 t
ORIGIN      
        1 ctaccaaatt gtctgaagag aaagggttct gagtttgccc tacctgcagg gtttggtcga
       61 actagacttg cccataggtg tcagccaaat atagtccgcg atgggttcct gcatgagcgc
      121 ccggacatcg gctgttttac cgggctcaaa tgacgtcatg tagatcgtga tctgtttgat
      181 gtcccctccc aggcgcctgg caactcgctt cggtacggga agattatatt gaatatgccc
      241 tcctcccgtg taactcacga tcatgcgatg ggagccaccg ttctcacggc gaaattcttc
      301 ctgcctgata gccagtgttc tcgccatgcc ctcatcccgg accatcgacg cctcatacat
      361 cgtacgaaag tgttcctcgg ttcctccgtg gcaccgtctc aattgatcga caatcctctc
      421 gcggtatgct ggatcgtcga cgatatcctc ctgtaggatt ccccacggcg cccattcagg
      481 ctcttgcctg gcctggtcaa gtcccaattt aacgacgcga cgaatcaagg gtttgggcgg
      541 gttcatcgcc cggacggaca agcgtcggtc tcgagcaaac gtcaccagtg gtgcatagtc
      601 ttcaaacgct ccaccccaat tttgtttcca gcgcacctct tccaaaaact catttgtgac
      661 cgcctgaggt gtcgcgacgt aaccgtcgag tgccggttgg ccgtcccaac cgaacatttc
      721 catcccaatg gtgggttcaa tgccatcagt caccaattga cccaacactt tcagtgctgc
      781 ttgtatatga tatggattgt gatgttcctc tcccagatag acaatgtcgt atcgttccaa
      841 gttcgtaatc aattctgaaa acggcagtac acggccggtt tgagtttcaa gaatctgcca
      901 gggttgccat tcctcatcga cgacggtacg aaggctgcca tcgcgctcag ccgcacagcc
      961 gatggggaat gccagcaggc ccacgagcaa gcccagcatg acggtcgatc gccaagccat
     1021 ggaattgccg gatagaaatg gtgttttcat gaatcactcc taccataccg aat
//
LOCUS       DASTWE010000371         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1367601, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000371 DASTWE010000000
VERSION     DASTWE010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367601"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1148)
                     /locus_tag="VFT30_03950"
     CDS             complement(<1..>1148)
                     /locus_tag="VFT30_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5091810.1"
                     /translation="HVHDLLSDEGEEFPDGRELARAMGHRTTLSVPLLREGQSIGTIS
                     LRRAEVHPFSTKQIELLQTFASQAVIAIANVQLFQEVQAKSGDLYEALSHQTASANIL
                     SVIASSPTDIKPVLHAIVESACQLCEAQDAIVRLRIGDFLLPGAHHGQIPINEDKWSI
                     NPGSVSGRAILNQRPIHLQDIQSAEGDEFPESQERARRQGHRSILSVPLMREGESVGV
                     IILRRVEAHKFSDKQVSLLQTFADQAVIAIENVRLFNETQETLERQTATADVLNVISR
                     SPTDVVPVFDVIGERAEKLCHAEISVVSILEGDVIRAAGIRGLSSAKVEAFRAHFPMT
                     LDRETVTARTIKSGSVIHIGDVLLDGTYDNKRLATETGYRSCLGVPMH"
BASE COUNT          249 a          305 c          308 g          286 t
ORIGIN      
        1 ctgtgcatcg gcacgccaag gcaactacga tagccggtct ctgtagctaa cctcttattg
       61 tcgtaggtac catcgaggag gacatcgccg atatgaatga ccgatccaga cttaattgtg
      121 cgggccgtaa cagtctcacg gtcgagcgtc atcggaaagt gggcgcggaa ggcttcaacc
      181 tttgcgctgc taagtccgcg aattcctgca gctctgatga catctccttc aaggatcgaa
      241 acgacgctga tttccgcatg acataatttt tcggcacgct caccgatgac gtcgaatacc
      301 ggaacaacat cggtcggaga ccggctgata acattcagta catcggcagt ggccgtctgc
      361 cgttcgagtg tttcctgagt ctcgttgaac agtcgcacgt tttcaattgc gatcaccgct
      421 tggtcagcga aggtctgaag aaggctgacc tgcttgtcac tgaacttgtg ggcctcgacc
      481 cggcgcagta ttattacgcc tacgctttcg ccctctctca tcaaaggcac actcagaatg
      541 cttctgtggc cttgtcgacg cgctctctct tgcgattccg ggaattcatc gccttcggcg
      601 gactgaatat cctgaaggtg tatcggtctc tggttcaaaa tcgctcggcc cgatacggag
      661 ccagggttga tggaccattt atcttcattt atggggattt gaccgtgatg ggccccaggg
      721 agaagaaaat cgccaattct cagccgcact attgcatctt gcgcttcgca taactgacag
      781 gcgctttcga caatcgcgtg cagtacgggt ttgatgtcag tcggtgaaga tgcaatgacg
      841 cttaaaatat tggcactggc agtctgatgt gagagcgcct cgtagaggtc gccgctcttc
      901 gcctggacct cctggaatag ttgcacattt gcgatcgcga ttacggcttg actggcgaaa
      961 gtttgcagca gttcgatctg tttcgtgctg aacgggtgca cttctgcacg gcggagggaa
     1021 attgtaccaa tactttgacc ctcgcgtagc aacggtacgc taagagtcgt gcgatgtccc
     1081 atcgcgcgcg ccaactcacg accgtcagga aattcttcgc cttcgtcgga aaggagatcg
     1141 tgaacatg
//
LOCUS       DASTWE010000372         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_753936, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000372 DASTWE010000000
VERSION     DASTWE010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753936"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>1552
                     /gene="asnB"
                     /locus_tag="VFT30_03955"
     CDS             21..>1552
                     /gene="asnB"
                     /locus_tag="VFT30_03955"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5091811.1"
                     /translation="MEGAFGLGHRRLSIIDVEGGAQPITNEDGTLQVIFNGEIYNYLE
                     LRKELVAFGHLFKTASDTEVILHAYEQWGASCVNRFNGMFAFAIVNARNNTMLLARDH
                     LGIKPLYYVVIGNVLLFASEIKALLQHSDCPRDVNLDSLAELFTFRYVPSPRTLFEGI
                     LKLPPGHVMHVDRYGTRVERFWTWVPRLRTRWEEQELIEEYQSLLEDAIRLQLRSDVP
                     LGLFLSSGIDSGVLLAIMSQYSSGKVQAFTIGFEGGEKTNEVADARALANMFGADHHF
                     EMVGPKDYLGYYESYLRDLEEPVGHEAASAFYFLAKGTSKYVKVALTGQGADEPWAGY
                     DRYIGVKLSNIYNRLPAMVTGPLARLLIRMPGRFERLKRGAVSLGEPDILTRFTKMYS
                     FFSAEMKEKLFKGSLRERSGDGYRSKEAIRRLQSDVQHLDPVTQMLYVDTRSSLPDDL
                     LMVGDKTSMANSLEVRVPFLDYRLIEFIEGLPPGLKLNGLTGKYLHKKALEKWLPKHI
                     IYR"
BASE COUNT          373 a          361 c          444 g          374 t
ORIGIN      
        1 cggatggtca gggatacttc gtggaggggg catttggctt agggcatcga cgactgagca
       61 tcatcgatgt agaaggggga gcgcagccga taactaatga ggatggaacg ctgcaggtta
      121 tatttaacgg tgaaatttac aattacctcg agttgcggaa ggaattggtg gcgttcggcc
      181 atctattcaa gacggcttcc gacaccgagg tgatcctaca tgcatatgaa cagtggggtg
      241 cgagctgtgt caatcgcttc aatgggatgt ttgcattcgc gatcgtcaat gcccgtaata
      301 acactatgtt actggcaagg gatcatctag gcatcaagcc gctctattac gtcgtgattg
      361 ggaacgtgct gctgtttgcc tcggaaatta aagctttgct tcagcactct gattgcccaa
      421 gagatgtcaa cctggattca ctcgctgagc tattcacatt tcgatatgtt ccgtcaccca
      481 gaacgctgtt cgagggtatt ttgaagcttc cgcccggaca tgtcatgcac gttgacaggt
      541 acgggacacg agtcgagcgg ttctggacct gggtgcctcg attgaggaca agatgggagg
      601 agcaggagct gatcgaagaa taccaatcgc tgcttgaaga cgccattcgc ctacaactaa
      661 ggagcgatgt tccactgggc ctctttctga gttctggcat tgactccggg gtgctgttgg
      721 ctatcatgag ccagtattcg tccggaaagg tccaagcttt tacgatcggc tttgagggag
      781 gcgaaaagac caatgaagtg gcagacgcga gagcgttggc caatatgttc ggggcggatc
      841 accacttcga gatggtgggg cccaaggact atttgggtta ttacgaaagc tatctccggg
      901 acctagagga gccggtcggg cacgaagccg caagcgcttt ctactttctg gcaaaaggaa
      961 caagtaagta tgtcaaggtg gctctgacag gtcagggagc ggatgagccg tgggcaggat
     1021 atgatcgtta catcggggtc aagctttcaa atatttataa ccgccttcca gcgatggtca
     1081 caggtcctct cgccagattg ttgatccgaa tgccaggacg attcgagcgt ttgaagcggg
     1141 gtgccgtctc cctgggtgag ccggacattc tgacacgatt cactaaaatg tactcattct
     1201 tcagtgcgga aatgaaagag aagcttttca agggaagttt aagggaacga tccggtgatg
     1261 gataccggtc gaaagaggcg attcgtcgac ttcaatccga cgttcaacat ctcgacccgg
     1321 taacccagat gctctatgtc gacacgcgat cgagtctgcc cgacgatctt ctcatggttg
     1381 gagataagac ctcgatggcc aattccctcg aagtccgtgt tcccttcctc gactatcggc
     1441 tgatcgagtt cattgagggg cttcctcccg gcctgaagct gaacggcttg acggggaagt
     1501 atctccacaa gaaagcgtta gagaaatggc ttccgaaaca catcatctat cg
//
LOCUS       DASTWE010000373         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1205892, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000373 DASTWE010000000
VERSION     DASTWE010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205892"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..>1408)
                     /locus_tag="VFT30_03960"
     CDS             complement(308..>1408)
                     /locus_tag="VFT30_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU5091812.1"
                     /translation="FKETPAKGPTPNCNWRGMPVFCGPKGLRAGINWLYRNRGDGTFS
                     DVSAKSGISKPTLNYGLGAVVSDFDHDGWPDIYVACDSSANLLFRNQHDGTFAELAIV
                     SGTAFNDDGMEQAGMGVSVGDYDHDGLFDLVKTNFADDTSTLYHNSGDGSFTDATFRA
                     GLGMNTRFLGWGTCFLDFDHDGWKDLFMGNGHVYPEVDARKLSVPYRQERLLYWNQRN
                     GTFVDVSAQAGPGILSRWSSRGVAIGDLDNDGSLEIVVNNMHDTPSLLKNYGEKTNWL
                     MVQTIGRRSNRNGIGARVTVQAGGLKQVDEVRSGGSYVSQSDLRLHFGLGDALRAEQI
                     EVEWPSGLKEAFQNLNANQLVVLEEGKGTKIP"
BASE COUNT          285 a          433 c          372 g          318 t
ORIGIN      
        1 gaggaaggcc ttgactcagc actcagatgc cagtcacatt tggcctcgat cttccgaccg
       61 tacgggacaa cccacgcgcc gtcggtgcgg ccccgttttt taagggggaa ttgctcgcaa
      121 ccacgtaatt tctacggcag ccgcaccctt ttgggtgttc ctgtcgccgg tgggtggagc
      181 ctgcaaccga cccaccccgg cgtcgcccgc cgccacccct ccaagagggg atttttgagg
      241 actccagggt tcagaatgcc gtcgtgacaa gactcgccga tgtcttaatt ccatcttcgt
      301 tccgcagtta cggaatcttt gttcccttcc cttcttcgag aactacgagt tggtttgcgt
      361 tcagattctg aaaggcctcc ttcagaccgc tgggccactc gacttcgatc tgttccgcgc
      421 gcagggcatc gccgagaccg aagtggagtc gcaggtcgct ttgcgaaacg tagcttccac
      481 cgcttcggac ctcgtccacc tgctttagtc ccccagcttg aaccgtcaca cgggcgccaa
      541 tcccgttccg attggaacgt cttccaatgg tttgcaccat gagccagttc gtcttttctc
      601 cataattttt gagcaggctc ggagtgtcat gcatgttgtt gacgacaatc tcgaggctgc
      661 catcgttgtc cagatccccg attgcaaccc ctcgagaact ccaccgggag aggattcccg
      721 gcccggcctg tgcagagaca tcgacaaaag tgccgttccg ctggttccaa tagagcaagc
      781 gctcctggcg atagggcaca ctcaatttcc gtgcatcgac ctcgggatac acatgaccat
      841 tgcccatgaa caggtccttc cagccatcgt gatcgaaatc gaggaagcag gttccccaac
      901 cgaggaagcg cgtgttcatt cccagtcccg cccgaaaggt tgcgtcagtg aacgaaccgt
      961 cgccgctgtt gtggtagagc gttgaagtgt catcggcgaa gttggtcttc accaggtcga
     1021 aaagaccatc atgatcgtag tcgcccacag agacgcccat gcccgcctgc tccatgccat
     1081 cgtcgttaaa cgcagttccc gaaacaatgg ccagctccgc aaaggtgccg tcatgctggt
     1141 tccgaaagag aaggttggct gatgaatcgc aggcaacgta gatgtcaggc cagccatcat
     1201 gatcgaaatc ggaaacgact gcaccaaggc cgtagttcaa cgtgggtttg ctgattccgc
     1261 tcttcgccga aacgtcgctg aacgtgccat ctcccctgtt tcgatagagc cagttgattc
     1321 ctgcgcgcaa ccccttcggc ccgcagaata ctggcatgcc gcgccaattg cagttgggcg
     1381 tcggtccctt ggcgggagtt tccttgaa
//
LOCUS       DASTWE010000374         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1270647, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000374 DASTWE010000000
VERSION     DASTWE010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270647"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFT30_03965"
     CDS             <1..515
                     /locus_tag="VFT30_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEU5091813.1"
                     /translation="HVDFTVAQQVAAGLQVGEAVEVLAAGDSPTVMARIVALDARVDP
                     TTRNAMIRARVEGTRNVPAPGASVRVRIPVGPQRTVVAIPISALRKGPGGDQVFVIAP
                     DKDGRNRVHIRQVESGPMFGDEIIVHAGLAAGEQIAAVGSFKLREGILIAVAEAVENN
                     SKGNHETGTP"
     gene            975..>1328
                     /locus_tag="VFT30_03970"
     CDS             975..>1328
                     /locus_tag="VFT30_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5091814.1"
                     /translation="MDHRLACKSLGALLIVVLSLSGCTELTHQHVDDPSRDVWQKPQG
                     VVEKLSLTRGSRVADLGAGGGYFTWHLAKAVGARGTVYAVDIEENAINTIFKEMVARG
                     TPNVRPVRAEPHDPRL"
BASE COUNT          295 a          395 c          371 g          267 t
ORIGIN      
        1 tgcatgtgga cttcacggtc gcgcagcaag tcgccgccgg cttgcaggtc ggcgaagccg
       61 tcgaagtgct cgcggccggt gattccccga ctgtcatggc cagaatcgtg gccctcgacg
      121 cgcgcgtcga tccgacgacc cggaatgcca tgatacgtgc cagagtcgaa ggcacccgca
      181 atgtaccggc acccggagct tcggtacgcg tccggattcc agtcggtcct caacgtacag
      241 tcgtcgccat accgatcagc gccttacgta agggacctgg aggcgaccaa gtcttcgtca
      301 tcgccccgga caaggacggc cgcaaccgag tccatatccg ccaggttgaa agcggcccca
      361 tgttcggtga cgagattatt gtccatgccg gcctggcagc cggcgaacaa attgccgcgg
      421 taggctcctt taagctccgc gaaggcattc tcatcgcggt cgcggaagct gtcgagaata
      481 actcaaaagg gaatcacgag accgggacac cctaaatcaa tcatcctgtg acaagacgcc
      541 acccaaacat cgttcaccga cgtgttcatc aaacttccag cgttggcgac ctcgttgacc
      601 aagctgcgcc tgatgctgat gacctccgtc gccatcttcg gtcatctccc actggtatag
      661 cgttggtcac cagcgtgaca gttggcatga tcttcacctg attggtcccg ccggtatttt
      721 attcactgat ccccgcgcag tatcagccgc ttggacaatc tgggacaccg gatgaagcga
      781 gcctgagggg gctgactttt gctggagcga gggcgtagac tatgttacaa ctaccgccaa
      841 ctagaccgtg attctccgta tgcttgcgtg agctgagacc tcggagggag caatctctcc
      901 cataaaacca actcatctcc tcatgcagta tccactttga ccgacccgaa accggtctag
      961 agaaagggaa gactatggac catcggttag catgcaagtc gttgggcgca ttgttgattg
     1021 tcgtgctctc tctcagcggt tgtaccgagt tgactcatca acacgtggac gatccctcgc
     1081 gggacgtctg gcagaaacca cagggagtcg ttgaaaaact gtccctcaca cgaggatcgc
     1141 gcgttgcaga cttgggagcc ggcggaggct actttacctg gcatctggcc aaggcagtcg
     1201 gggcaagagg aacggtttat gcggttgata tcgaggagaa cgcaattaac acgatcttca
     1261 aagaaatggt cgcccgaggg acccccaatg tccgaccggt ccgggccgaa ccgcacgatc
     1321 caagactc
//
LOCUS       DASTWE010000375         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_979995, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000375 DASTWE010000000
VERSION     DASTWE010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979995"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFT30_03975"
     CDS             <1..1100
                     /locus_tag="VFT30_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HEU5091815.1"
                     /translation="IFLCLLMVSASTACTWFTGKGKPGWVNGRSAEYPSAQYLTGVGQ
                     ADTRSNAEDQAYAAVARIFKAEVAAQAKDWESYLVVENRGVTNAERRLTIDNVTKVST
                     DKVLENVGIADVWYDSDSRLYYALGVMNRSQAETALMEKVSVLDRAIDADVAESRQAT
                     DKLAKVRALRRAGRNLVLREAYNTDLRVIRVSGKGTSSAYQVNELSGELEQFLATNLV
                     LEVQVSGDQAEPVGRALAEGLIREGLRVTTTKTEREEAGSPELIVRGTVRLFPIEVRD
                     PNFKYVRWCSDFEIVEPETRRVVGATAHGGREGHLTEREAMAKTLRVMQHELSSDVAK
                     AIAAHIFGEVALPETATMPASCPREASEVRR"
     gene            1131..1400
                     /locus_tag="VFT30_03980"
     CDS             1131..1400
                     /locus_tag="VFT30_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091816.1"
                     /translation="MKKTDELSVSRAPRSRGRGLTKSGQKISSRLAKRRLNDRLKDDT
                     SSFNNGNLADTFRKEGYEEVPLDELQDRLSKLQGPLAEFILKGRV"
     gene            1405..1848
                     /locus_tag="VFT30_03985"
     CDS             1405..1848
                     /locus_tag="VFT30_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5091817.1"
                     /translation="MPYYYFDSTALIKRYSMERGTRIVNKLMVKRGKVAIVPTWTVTD
                     FYSILCARAQEGQITRDDCYSVLYKFEIESKQGLYQFIAPKMETYLATKELVLEYPFL
                     RSPQVMHLALALELKPLRLTVVSADTQLLAASKTAGLHIINPAED"
     gene            complement(2046..>2887)
                     /locus_tag="VFT30_03990"
     CDS             complement(2046..>2887)
                     /locus_tag="VFT30_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEU5091818.1"
                     /translation="SLLDNDTYSFDEALPALAALRLENIPVILVSGKTRAEIEPLRER
                     LDHHDPFIVENGAAVFVPLNTFDFPLERSRRRSNYHIIELGTPYALLRDVLRQIEEAV
                     GTPLQGFGDLSVDEVMATTGLSREDALRAMLREFDEPFLVNGPPKLIEEVCRQIVTRG
                     LSWTRGGRFFHLTGNNDKGRATEILLRCYKRQGRLANLPDDIETIGIGDSLNDLPLLL
                     TVDHPVLVQKPDGSYDPDINLPNLIRAPGIGPAGWNRAVLELLRQASEETSLGRSANI
                     RAS"
BASE COUNT          662 a          733 c          894 g          598 t
ORIGIN      
        1 cgattttcct gtgtctgttg atggtctccg catcgaccgc ctgtacctgg ttcaccggaa
       61 aagggaaacc ggggtgggtc aatggccgga gcgcggaata tccatcggct caatacctca
      121 ccggggtcgg ccaagcagac acccgaagca atgcagagga tcaggcgtat gcggcggtgg
      181 ctcgcatctt caaggcggaa gtcgcggcgc aggctaagga ctgggaatcg tatttggtcg
      241 ttgagaatcg cggtgtcacc aacgccgagc ggcggctcac gatcgacaac gtgacgaaag
      301 tatcgaccga caaagtgctt gaaaacgtcg ggattgcgga tgtgtggtat gactcggaca
      361 gccggttgta ctacgctctg ggcgtgatga atcgctctca agccgaaacc gcgttgatgg
      421 agaaggtatc ggtgttggat cgcgcgatcg acgcggatgt cgccgagtca cggcaagcga
      481 ccgacaagct tgccaaggtt cgggcccttc ggagggccgg cagaaatctc gttctacgcg
      541 aggcatacaa cacggatttg cgagtgattc gtgtcagcgg taaaggtacc tcctccgcct
      601 atcaggtaaa cgaattatct ggtgagttgg agcaatttct tgctacgaat ctggtgcttg
      661 aggtacaagt gtccggtgac caggcggaac cggttggacg tgcgttggcg gaagggctta
      721 tccgggaagg acttcgcgtg accacgacga agacagaacg tgaagaggct ggttccccgg
      781 aattgatcgt ccgaggcact gtccgtctat tccccatcga ggtgcgggac ccgaatttca
      841 agtacgtgcg atggtgtagt gatttcgaga tcgtggaacc ggagacgcgg cgggtcgttg
      901 gggctacggc gcacggtggg agggaaggcc acctgacgga gcgtgaggca atggcaaaga
      961 cgctgcgcgt gatgcaacac gagttgtcgt cggatgtcgc gaaggcgatt gccgcccata
     1021 tcttcggcga ggtcgcgttg cctgagacgg cgacgatgcc ggcgagttgt ccacgagaag
     1081 catcagaggt gaggcggtaa tgaacgttct ttcatgagga ggcatcgatc gtgaaaaaga
     1141 cagacgaact atccgtcagc cgagctccac gcagcagagg acgagggttg accaagtcag
     1201 gacaaaagat ttcaagtcgt ttggccaagc ggcgattgaa cgaccgactg aaagacgaca
     1261 cgtcgtcctt taataatggc aatctcgccg acacctttcg aaaagaaggg tatgaagagg
     1321 tgccgctcga tgagctgcaa gataggctct ccaagctcca agggccgctc gcggaattca
     1381 ttctgaaggg gagggtgtaa gggaatgcca tactactatt tcgactcaac cgcactcatt
     1441 aagcgctaca gcatggagcg gggcacgaga atcgtgaata agctgatggt aaagcgcggg
     1501 aaggttgcga tcgtaccgac atggaccgtg acggacttct attcgattct gtgcgcacgt
     1561 gctcaagaag gacagattac ccgggatgat tgctactcgg ttctttataa gttcgaaatc
     1621 gaatccaagc agggactgta ccaatttatc gcaccgaaga tggaaaccta cctggccact
     1681 aaggaattgg tcttggagta tccgttccta cgatcgcctc aggtgatgca tctggcgttg
     1741 gcattggagc tcaagccgtt gcgattgacc gtcgtcagcg cggataccca gttattggcg
     1801 gcgtcaaaaa ctgcaggact gcatatcatt aatccggcgg aagactagca gactgttgaa
     1861 aaagtccgca gctgcgtttt cgccaagata ctgccgcctc accaagacac ggggcgttca
     1921 cgggacacgg cccgctcact gtctcgcggg cgttcacatg ggcgagtgag aatgacggcc
     1981 tttttgaaca gcctgcgact tttgtgagag gatctgaaac ctctggatct ccatcctgcg
     2041 ggcgatcagc tagctcggat gttggcggat cggccgagcg aggtctcttc agaggcctgt
     2101 cgtagcaatt ccaagacggc acgattccaa ccagcagggc cgatgcctgg cgcgcgaatg
     2161 aggttcggca ggttgatgtc aggatcgtat gagccgtcag gtttttgcac caacaccgga
     2221 tgatcgaccg tcagtaagag agggagatcg ttcaggctat cgccgatgcc gatggtttct
     2281 atgtcgtccg gcaggttagc cagtcgccct tggcgtttgt agcagcgaag aagaatttct
     2341 gtcgcccgac ctttgtcgtt gtttcccgtc aagtgaaaga aacgcccacc tctcgtccaa
     2401 ctaagacctc gcgttacgat ctgacggcag acttcctcga tcaacttggg ggggccgttc
     2461 accaaaaacg gctcgtcgaa ttcccgcagc atggcccgca gtgcatcttc tcgggagagt
     2521 cccgtggtcg ccatgacttc atcgaccgac agatcgccga atccttggag cggcgtaccc
     2581 accgcttctt cgatttgccg gagtacatcc cggaggagag catagggcgt acccagttcg
     2641 atgatatggt aattggagcg gcgtcgcgac cgctccaggg ggaagtcaaa ggtattgagg
     2701 ggcacaaaaa ccgctgcgcc attctcgacg atgaagggat cgtggtgatc gaggcgctct
     2761 cgaagcggtt cgatttccgc acgggtcttt ccggagacga gaatgactgg aatgttctcc
     2821 aaacgaaggg ctgcgagcgc cggcagcgcc tcgtcaaagg aatacgtgtc gttatccaac
     2881 agagagc
//
LOCUS       DASTWE010000376         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_624189, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000376 DASTWE010000000
VERSION     DASTWE010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624189"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFT30_03995"
     CDS             <1..455
                     /locus_tag="VFT30_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEU5091819.1"
                     /translation="LTNSRFLDLYAGTGAVGIEAVSRGAVHVTSVESNRDALKLMRQN
                     LELCHIGDEVAVLDRTIQQFLNHPDQWTGPYDIVFADPPYAEASLLPSLFAEPRIDDL
                     FALDSWLVIEHAAKTVLPMSLGCTKFSRRYRYGDTALSLYFRSHTVQA"
     gene            452..940
                     /gene="coaD"
                     /locus_tag="VFT30_04000"
     CDS             452..940
                     /gene="coaD"
                     /locus_tag="VFT30_04000"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5091820.1"
                     /translation="MKIGIYPGTFDPITHGHTDIITRSLRVFDKVVVAVAPNPSKHPL
                     FNLAERLDMVKLVMKDVGKVEVTPFDGLLVNYVEHSGAHAIIRGLRAISDFEHEFQMA
                     LVNRKLAKMVETVFLMPSEEYSYLSSTIIKDVAQHGGNLIDFLHPEVAQRLQARIRSL
                     KS"
     gene            937..>1126
                     /locus_tag="VFT30_04005"
     CDS             937..>1126
                     /locus_tag="VFT30_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HEU5091821.1"
                     /translation="MKLAARVGRIAPSPTLAMAATAKAMAAQGLDVIDFSAGEPDFDT
                     PEPVKAAAEAAIREGFTKY"
BASE COUNT          255 a          307 c          298 g          266 t
ORIGIN      
        1 ggcttacgaa cagccggttt ttggacctct atgctgggac cggagctgtg ggcatcgaag
       61 ccgtcagtcg tggtgccgtg cacgtgacct ccgtggagtc gaaccgagat gcattgaaac
      121 tgatgcggca aaacctggaa ctctgtcata tcggtgatga agtagccgtg ctggatcgga
      181 cgatccaaca atttctgaac catcccgatc agtggaccgg tccttatgat attgtctttg
      241 ccgacccacc ctatgccgaa gcgtcgctcc ttccatcgtt attcgctgaa ccaaggatcg
      301 atgatttgtt tgccctggac tcatggctgg tcatcgaaca tgctgcgaag accgtcttac
      361 caatgtcgct gggatgcacc aagttctcac gacgatatcg ctacggggat acggccttat
      421 ccctctactt ccgttcccac accgtgcagg catgaaaatc ggtatctacc caggcacttt
      481 tgatccgatt acgcatggcc acaccgacat cattacccgc agcctccgtg tgttcgacaa
      541 ggtggtagtg gctgtggccc cgaatccctc caaacatcca ctcttcaatt tggcggaacg
      601 ccttgacatg gtgaagctgg tcatgaagga tgtaggaaag gtcgaggtca caccgttcga
      661 cggattgctc gttaattatg tcgagcattc aggtgcccat gcgatcatcc gtggtttgcg
      721 tgccatttct gactttgagc atgagtttca gatggcgctc gtcaaccgaa aacttgccaa
      781 gatggtggag accgtgttct tgatgcccag cgaagagtac tcatacttgt cctcgacgat
      841 catcaaggac gtggcacaac acggtggaaa tctaatcgac tttcttcatc cggaagtagc
      901 tcagcgccta caagctcgta tccggagtct caaatcatga aattggcagc acgtgtcggt
      961 cgtattgcac catcccccac gctggccatg gcggcaaccg ccaaggccat ggcggcgcaa
     1021 ggactggatg tcatcgactt ttcagctgga gaaccggatt ttgacacacc ggagccggtg
     1081 aaggctgcgg cagaagcggc catacgcgag gggtttacca aatata
//
LOCUS       DASTWE010000377         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_398324, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000377 DASTWE010000000
VERSION     DASTWE010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398324"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFT30_04010"
     CDS             <1..416
                     /locus_tag="VFT30_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091822.1"
                     /translation="ADQQSLKLAILALVVLLLGGVVLSVQFETWLAEVRRMPVESARK
                     SLTTVFSWSVGIVTVAIALAGCHVWRWGRRVRQTLRFPPPGATVVRDTVVLIGRSAES
                     RGTLLQVLGVTLILCAAGVAVVSWWVLRMLGNIHG"
     gene            435..1265
                     /locus_tag="VFT30_04015"
     CDS             435..1265
                     /locus_tag="VFT30_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091823.1"
                     /translation="MMPQSASPRIYCIPAIQVPVVAVFRRGPSNWSHIGRWDLAAGQY
                     EPGAWLGGRIFPRRSDLSPDGQYLCYFAHKPSAMWEHGEAYVALSKLPWLTALHAFAT
                     CGTWTRGYYFTEDRGRDDQQKDRLPIPYGLRSISVVQFANERRRGWIEAEDSPVRDPA
                     DTWDERRNARMRKPQPHGTRVLCVESVGVGGGEFGVDQSVDGLNVRYSLESNGDLELL
                     DDVQWADWSKDGELLVATRSGRLQIQNLESDDSKPLFEMDLSNLEPHPRPAPAWARNW
                     "
BASE COUNT          342 a          423 c          470 g          388 t
ORIGIN      
        1 gagccgacca gcagagtctg aagttggcga ttctcgcgct ggtggtgttg ttactggggg
       61 gtgttgtgct ctcggttcag tttgagacat ggttggcgga agtgcgtcgc atgccggttg
      121 aatccgctcg aaaatccctc acgacggttt tcagttggag cgtgggaatt gtgactgtcg
      181 caatcgctct tgccggttgt catgtttggc ggtgggggag acgtgtccgg caaactctgc
      241 gattccctcc accaggagca acagtcgtgc gagatacggt ggttttgatt ggaagatcgg
      301 cagaatccag ggggacattg ctccaagttc tcggcgtcac gctcatcctc tgtgcggcag
      361 gagtcgccgt tgtgtcatgg tgggtgctca ggatgctcgg caatattcat ggctgaacct
      421 ctgtagagag atcaatgatg ccacaatcgg cctcgcctcg tatctactgc attccagcaa
      481 ttcaggtgcc ggtggtggca gtgtttcgtc gtgggccgag taactggtcg catatcggcc
      541 ggtgggatct ggccgcaggg caatacgaac cgggagcctg gctcggcggc cgcatctttc
      601 cgcgtcgatc cgacctttct ccagacggac aatacctctg ctacttcgcg cataaaccga
      661 gcgcgatgtg ggaacatggc gaggcatatg tcgccctgtc gaaacttccg tggcttactg
      721 cattacatgc gtttgcaact tgcggtacct ggacgcgcgg atactacttc acagaagacc
      781 gcggtcgcga cgatcagcaa aaggataggc tcccgattcc ttacgggtta cgctcgatct
      841 ccgtggttca attcgccaac gagcgacgac gtgggtggat agaagcggag gactcaccgg
      901 tccgtgatcc agcagacaca tgggacgagc gccgcaacgc tcgcatgcgg aagccacagc
      961 cgcatggcac ccgagtcctc tgtgtggaaa gcgtgggcgt tggcggaggg gagttcggtg
     1021 tcgaccaatc cgtcgacggt ctgaacgtca gatattcgct ggaatccaac ggcgatctcg
     1081 aactcttgga tgatgtccaa tgggccgact ggagcaagga tggggagttg ttagtcgcga
     1141 cacgatcggg ccggctgcaa atacaaaatc ttgaaagcga tgattctaaa cctctctttg
     1201 agatggatct ctcgaaccta gaaccacatc ctagaccggc gccagcttgg gctcggaact
     1261 ggtagcggca attccagtac atcttccgac tcaagcaata ttagctgcat gccctgaaca
     1321 attccaatca tcacgttaaa catgcaaaga tttttgcaca atgcacactg cctcccttgc
     1381 tacagcctgt gcatcggttt aggattaatg aaaatgcatg gaccatcgga tccgcgttat
     1441 cgcgttatat ggaatggctt ttctacattc ttatcaggaa tccattttgt tagtatagcg
     1501 atttgttatg agtcacccgc tggatttcgt caccattgaa ggactccttg cctatggtcg
     1561 cgccctcgct cgacgagctt ctgagcggag actcctggag cctccgtcag tagaagcttc
     1621 cga
//
LOCUS       DASTWE010000378         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_850654, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000378 DASTWE010000000
VERSION     DASTWE010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850654"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..828)
                     /locus_tag="VFT30_04020"
     CDS             complement(40..828)
                     /locus_tag="VFT30_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091824.1"
                     /translation="MQWASLWRPKPLLRVCLVLALASCAQEAQSPPKARVQPGMHTVE
                     PADQWAFAEEEAEMDPLVAAFDPLTGAGESEQIDAQFRGRDRKAAKTSISSAPIEEFS
                     SLEELLQTLASDDDMRNHNPEITRASTSQRVAEENRNVRVPAFICAIKYEADQDWHII
                     GSNDSECDGPFFNFEVSGLPRRNSSAHDTLLTVRQDLAEILDHDLPGPGSYHKYKGSG
                     PIPVIIQGSLFYDVDHPPGVVGPNTMKPETAWEIHPVTEISLDQ"
BASE COUNT          193 a          300 c          297 g          224 t
ORIGIN      
        1 agacacgagg gccagatgcg tgatcattcc aaagcccctc tattgatcca atgagatctc
       61 cgtgaccggg tgtatctccc acgcggtctc aggcttcatg gtgttgggtc ccaccacgcc
      121 tggcggatga tcaacgtcgt agaacagcga cccttgaatg atgaccggaa tggggccgct
      181 gcccttgtat ttgtgatagc tccccgggcc gggcaaatcg tgatcgagga tttcagcgag
      241 atcctgccgg accgtcaaca acgtgtcgtg ggcggagctg ttcctcctgg gaaggccgct
      301 gacttcgaaa ttgaagaatg gaccgtcgca ctccgaatcg ttcgaaccga tgatgtgcca
      361 gtcctgatcg gcctcgtact tgatggcaca tatgaatgcg ggaacccgga cattcctgtt
      421 ctcctcggca accctctgcg aggttgaggc gcgcgtgatt tccggattgt gattccgcat
      481 gtcgtcgtct gacgccaacg tttgcagaag ctcttcaaga gacgagaact cttcgatggg
      541 cgccgacgag atgctcgtct ttgccgcctt gcgatccctg cctctgaact gagcgtcaat
      601 ctgctcggac tccccggcgc ccgtcagagg gtcgaaggcc gcgacgagag ggtccatctc
      661 cgcctcttct tcggcaaacg cccattggtc tgccggttca acggtatgca ttcctggttg
      721 gaccctggcc ttgggcggac tctgcgcctc ctgagcgcag ctcgccaaag ccagcacgag
      781 gcagacccga agaaggggtt tcggtcgcca tagcgatgcc cactgcatgt tgacctccct
      841 aagcacgctg tcgtcggagc agccgcgcga accgagcgtc gttagactct gacgccagcc
      901 gaaggatcta gaggtcgtat caacgatttg ttggatcgtt ggcagtctac ctgctcggtg
      961 cgccggtagc aatcacagat tgttgtcgca tggtgttctt caacgggagg ccac
//
LOCUS       DASTWE010000379         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_818483, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000379 DASTWE010000000
VERSION     DASTWE010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818483"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..551
                     /locus_tag="VFT30_04025"
     CDS             351..551
                     /locus_tag="VFT30_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEU5091825.1"
                     /translation="MSVAKIIEISAESSTSFEDAIVQGIAKASKTIHSIKSAWVKEQQ
                     VVVESGKVVLYRVDLKVTFVLD"
     gene            700..1242
                     /locus_tag="VFT30_04030"
     CDS             700..1242
                     /locus_tag="VFT30_04030"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5091826.1"
                     /translation="MEAVRETVTEVLRILNDPALKNPAKHAQRRRMLEEIATSRFDYA
                     EMSKRVLGSYWKPLTESQRKEFVEVYKSFLADKYAGRIEDYSGRKQEVGYLTERIEGS
                     YAEVRTELRSDKTTFPMDYRLLVKDGRWCAYDIIIDGVSLVSNYRSQFQKIMRDSSYE
                     ELVKKLRERSIATENTKAKP"
     gene            1352..1522
                     /locus_tag="VFT30_04035"
     CDS             1352..1522
                     /locus_tag="VFT30_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091827.1"
                     /translation="MTQLSLEPNEQDILIWALQSSVSDLGGEIADTENQEFREDLKQR
                     KSVLQNILRRLR"
     gene            complement(2133..>2697)
                     /locus_tag="VFT30_04040"
     CDS             complement(2133..>2697)
                     /locus_tag="VFT30_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5091828.1"
                     /translation="PDEIYFLAQEVGPEWYKQLVHPQLLSAVRGVVANYTMVTLPERS
                     SEIGNKIEAVVVETLKGRHLDVYNVALSEMEFSQMVLRAIEQKQAKEQEKEQKDFEVV
                     IAQRNAEIARIQAKGEGDSLKIRAEGEADSMRIRAIGQSQAQEIITKTLTPDYLRFKL
                     YESPNAKTIIVPEKLNIPMIVNPGADR"
BASE COUNT          648 a          673 c          728 g          648 t
ORIGIN      
        1 actgaattca caagaatatc tatttaggtt tacaaaccag tcgcgacata gtctgcgacc
       61 ataggtcgga agcgagaaga ggagtgcact gagaaccttg ttgatccgat ccattcggca
      121 atgcggcatg cacattattg ctacagatct ttttcattag gtgtagcaga gttccccaat
      181 cctgttcgcc ttgtttccaa gcttctcaat aatgcgctga aattcctgag atattgctta
      241 agtgccggac tactgaccga tcgcgttctg gcgtcgactt tgctgatcat gagcgtgggt
      301 cacctggatc ataacgtttg gtggacatga acaggaaagg gaggagctct atgtctgtcg
      361 ctaaaatcat tgagatcagc gccgaatcgt ccacgagttt cgaagatgcc atcgtccaag
      421 gaatagcgaa agcatccaag acaatccaca gcatcaaatc agcgtgggtg aaggagcagc
      481 aggtcgtggt cgaaagtggt aaggtcgttt tgtatcgtgt agatctaaag gtgacgttcg
      541 tattagattg acgagttagg aagaaaccta tacgtcatgc ttcagtggcc aggaaggagt
      601 cggcgagttg caaagggttc agtgtggcct gcggcttttg tctgcgcttt tttctcgtgc
      661 tttgctgtca ccgcttcctc gcaaggcacg gaaaccccca tggaagcggt gcgcgagacg
      721 gtcaccgagg tcctccgcat cctcaatgat cccgcattga agaacccagc caagcacgca
      781 caacgtcgtc gcatgctgga agagatagcc actagcaggt ttgactatgc ggaaatgtcg
      841 aagcgagtgc tgggcagtta ttggaagccg ctgacggaat ctcagcggaa ggaattcgtt
      901 gaggtataca agagttttct cgccgataag tatgccggaa gaatcgagga ttatagcgga
      961 cgaaaacaag aagttggcta cctgacggag cggatcgaag ggtcatatgc ggaagtaaga
     1021 accgaattgc ggtcggacaa aacgacgttt ccgatggatt accgcctctt ggtgaaggat
     1081 ggtcggtggt gtgcctacga catcatcatc gacggcgtaa gtttggtgag taactatcga
     1141 agtcagtttc agaagatcat gcgcgacagc tcctatgagg aactggtcaa gaagttgcgc
     1201 gaacgatcca tcgcgacgga gaacacgaaa gcgaaaccat gaccggagcg gaggacggca
     1261 ctcacaccag ttcatgggta atccggcgaa catcctgtag aaaggtggcg gcacagatct
     1321 atagagcgta agtgagaagg aggaaaggct tatgacgcag ctatcgctgg agccgaacga
     1381 acaggatatc ttgatctggg cgcttcaaag ctccgtatct gatctcggcg gagaaatagc
     1441 ggacacggag aatcaagaat ttcgggaaga ccttaagcaa cgaaaatcgg tcttacagaa
     1501 tatcctgcga cgcttgcgat aaggccacat gctgcttcgc cggagacact tcgatcacgg
     1561 gatcgatgag catcacggaa gtatgcagcg tgtaaggcca gaacaacaac taagggcccg
     1621 agtccaaaat tctgttcatt cacgtacatc atgatcgtga tcccctcgca acttcgctgt
     1681 ttcgttatct tccccgccat tgactgaggg ctgtttggtc ttcgtggtgg cagacgacct
     1741 cgaagaactg acatggcatc gatgctcctc ctcgtgaatc ccggccgtct gaatgctgcg
     1801 ctcggggtat aatgctaggc tgttttttgc accttcactt cgtcaagtga tagcccgagt
     1861 cagtaagcgt tttcattacc gcgtactttt gaccgacact cgcgtcgctc tctaatgcaa
     1921 acacgacggc cttatcccgc actgtttgta tcgcagcagg cgctagtttt gaaggcgtcc
     1981 gagccgagca tggggtgaga gatggcagta tgatgctcag cataggcatc aagcagccgc
     2041 ggatcatgcg ctcggtactg cgtgtgatgt ttccgcgcct caccctggaa gaattcgacg
     2101 cttctcttgc ccaatgcaat actatccgcc tcttatcgat cggcgccggg gttgacaatc
     2161 atcggaatat tcagcttttc aggcacgatg atcgtcttgg cattggggct ttcatagagc
     2221 ttgaaccgca agtagtccgg cgtcagcgtt ttggtgatga tctcctgcgc ctgcgattgt
     2281 cctatggccc ggatcctcat gctgtcggct tcgccttccg cgcgaatctt caacgaatca
     2341 ccctcgccct tggcctgaat ccgggcaatc tcggcattgc gctgcgcaat cacgacctcg
     2401 aagtcctttt gttctttttc ctgctccttc gcctgcttct gctcgatagc cctcagcacc
     2461 atctgtgaaa attccatctc ggaaagtgcc acgttgtaga catccaaatg gcgacctttt
     2521 agtgtttcga cgacgacggc ttcaatctta ttgccgatct cgctgcttcg ttcgggaagc
     2581 gtgaccatgg tgtagttggc gacgacccca cgaacagccg acaagagttg gggatgaacg
     2641 agttgcttgt accactcagg ccctacttcc tgggccaaaa agtagatttc atcgggc
//
LOCUS       DASTWE010000380         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_139814, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000380 DASTWE010000000
VERSION     DASTWE010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139814"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..>1265
                     /locus_tag="VFT30_04045"
     CDS             328..>1265
                     /locus_tag="VFT30_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5091829.1"
                     /translation="MKSKSSTIAIGIALLVVLIIAVRWGGWSTSESSTAAPATNQLIL
                     VEVAPVIVGSMTESIQAVGTLEAIASITVRPEIAGVIRRIHFQDGQIVERAAPLLELD
                     PEELQSQVNQAAAEEKMTLVTYKRLKRLNDQQEVIVPAQQIDEARMAWQAAASNSLLY
                     AARLKKTIIRAPFSGTVGLRRVSVGDYIQPGQAIVNLEDLSTLHVDFKVAEVWLSRLH
                     VDQQLMLTTDAFPDTTFTGHVTAIDPRVDATNRTVAVRAAIPNPAGRLRPGLFVTVRL
                     NVGEDSRALLIPEEAGFLRQEKAMVFQVEDRTARLK"
BASE COUNT          284 a          320 c          386 g          275 t
ORIGIN      
        1 tatcagagat cttggtaaag agcgccgggc tggcgttatc gccacacgcc tggtgttgta
       61 cactcactgg cggtgacgga ctgagcgcac aaggcggcgt ggggtggatc tgactaccac
      121 caaaggcaat tcaggagcgc aaaagcagca atgtcaagcg gagggaggta gtatcttccg
      181 accgtctcta aaaaccgtcc actgcaagcg gaataaatca gcaggtgagg tttctgtccg
      241 gttttttcgt ctcatatacg actaggactg gtgtgtccgt ctgaccaccg tgggacaaga
      301 tccttcggct agaattgact gacgaatatg aagtccaaaa gttccaccat cgctattggg
      361 atcgctctgc tggtcgtcct catcattgcg gtcaggtggg ggggctggtc aaccagcgag
      421 tcatcgacgg ccgcgcctgc gactaaccag cttattttgg tagaagtcgc gcccgtcatc
      481 gtcggttcaa tgacagaatc gatccaggcc gtggggacac tggaggcaat cgcctcgatc
      541 accgtgagac ctgagattgc cggcgtcatt cgccgaatcc attttcaaga cggacagatt
      601 gtcgaacgtg cggctccatt gctggaattg gaccctgagg aactccagtc gcaagtcaat
      661 caggcggcgg cggaggagaa aatgacgctg gtgacgtaca agcggctaaa gcgactgaac
      721 gatcagcaag aggtcattgt gccggctcag cagattgatg aagccagaat ggcttggcaa
      781 gcggcggcgt cgaacagcct cctgtatgcc gctcgcctca agaaaaccat catccgtgcg
      841 ccgttcagcg gaacggtggg tcttcgtcgc gtctcggtcg gggattatat ccagcctggg
      901 caagccatcg tgaatctgga ggatttaagt actctccacg tggatttcaa agtggctgaa
      961 gtatggttga gtcgtctgca tgtcgaccaa cagctgatgc tgacgactga tgcgttccct
     1021 gacacgacgt ttaccggaca tgtgacagcg atcgatccga gagtcgatgc gactaaccgg
     1081 acggtcgcgg ttcgggcggc gattccgaat ccggcgggta gactgcgtcc cggtctcttt
     1141 gtgactgttc ggctgaatgt aggggaagat tcccgtgcgt tgctcatccc tgaagaggcg
     1201 ggatttcttc gacaggagaa ggccatggta tttcaggtcg aggataggac tgcccggctg
     1261 aaaga
//
LOCUS       DASTWE010000381         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_204602, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000381 DASTWE010000000
VERSION     DASTWE010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204602"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..881
                     /locus_tag="VFT30_04050"
     CDS             9..881
                     /locus_tag="VFT30_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5091830.1"
                     /translation="MALGCMRIARLSNQDIARLIHTALDAGIAFFDHADVYGGGKSEE
                     KFAEALDMTPRLRDTMILQSKVGIRQGFFDFSKEHILEAVEGSLKRLRTDHLDVLLLH
                     RPDALVEPEEVAEAFTILQDSGKVKYFGVSNQNPMQIELLTKFVKQPLIINQLQLSIT
                     NTGMIDAGINVNMKIDSSIVRDGSILDYCRLKEITIQPWSPFQYGFFEGVFLDNDKFP
                     ELNAQINTIASSRGVTNTAIAIAWLLRHPARMQPILGTTNPTRVKDASKASDVKLTRQ
                     EWYAIYLAAGNKLP"
BASE COUNT          243 a          273 c          268 g          231 t
ORIGIN      
        1 tttccgaaat tgcacttggc tgtatgcgga ttgctcgtct gtccaatcag gatattgcga
       61 ggttgatcca cacagcgctg gacgcaggta tcgccttttt cgaccatgcc gatgtctatg
      121 gcggcggcaa gtcagaggaa aagtttgccg aagcgcttga tatgacaccg cgcctccgcg
      181 acaccatgat cctccagagc aaggtcggga ttcgtcaggg cttcttcgat ttttcgaaag
      241 aacacatttt ggaagcggtc gagggcagtc tcaagcggct gcgaaccgat catctcgatg
      301 ttttgctcct gcatcgccca gatgcgctcg ttgagccaga agaagtagcc gaagccttca
      361 cgatcctgca agacagcggc aaagtcaagt attttggtgt gagcaaccaa aacccgatgc
      421 aaattgaact gctgaccaaa tttgtcaagc agccgcttat catcaatcaa cttcaactga
      481 gcatcaccaa tactggcatg atcgatgcgg gtatcaacgt caacatgaag atcgattcgt
      541 cgattgttcg cgacggcagc atcctcgatt actgccgcct gaaggaaatc actatccagc
      601 cgtggtcgcc ctttcagtat ggcttctttg aaggcgtctt tctagacaat gacaaattcc
      661 ctgaactgaa tgcacagatc aatacaattg cgtcatcccg aggggtcacc aacacggcaa
      721 tcgcgatcgc gtggctgttg cggcatcctg cacgaatgca gcccatcctt ggtacaacga
      781 atccaacccg tgtaaaagat gccagcaaag catctgatgt caagctgacc cgccaagaat
      841 ggtatgcgat ttatttggcc gccgggaaca aattgccctg acctggcggc tcattgttcc
      901 cggtatgttc tggaggtgag cagaatggcc taaatgggcg gcgggggaag cgccgggctg
      961 ccacggtctt ctgtcggccg aagccgggca tggctgttcc gcactacgga cggcc
//
LOCUS       DASTWE010000382         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1270964, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000382 DASTWE010000000
VERSION     DASTWE010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270964"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>2067
                     /locus_tag="VFT30_04055"
     CDS             40..>2067
                     /locus_tag="VFT30_04055"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEU5091831.1"
                     /translation="MRFDNASFMKGAADTQKSLADLNKGVDAAGNTKGLTNLSDQMGQ
                     VSISASKMQVATVAAIGTIAAKATALGLNVIKGLTFEPIAAGFAEYEENLNKLNTIMN
                     ATGKSEKVVQGVLTNLNAYSDKTIYSFSNMTTSIQKFVNAGVPLGESVEAIKGIANAA
                     AYSGATTEEANRAMFAFSQTMGTGFMMLNDWQQIENANMGTVKFKQTLIDTAVAAGTL
                     TKQGNAYVTSTGKTVSATKGWRESLQEQWATTEVVTGALSKYTDTQTKLGRAATESAM
                     EYRTFTAFFDSFKESLGSGWAKIFDALIGGLDEATHFWTSFANSVTGVTGAFFEFTAN
                     ALKTWRTMGGFEKTIQGFKNLLAPLGAIFRVVGAALSAAFPSGNKGVGKTLYTMSAGF
                     EAITRPLQWFADVIEGTTPVIATFFRMLHLIGAAIKSAGGTVANFVKDLLGMVSIQVP
                     SGSGFIGFIKDLAKAVSDASKKVDELVQKGASVGAAFGAVDFKMPSMPDMPSMPKLSM
                     PSLGGGDKAASGVSTMSASVGDLTGNVSRLKSVSDSLKNSTIFNSATMASAKDSFRSV
                     GDGAKDAAGGVVSAGEWIKNAWGSVVDFLGPIFSKIKDFIGGISADDIVSSFNMAIFA
                     TMGIQLAKFIHTLNNMLDMVNSFAQIGPAFTNVLDSAGGALKSFQTQAQAKL"
BASE COUNT          452 a          566 c          603 g          446 t
ORIGIN      
        1 tgtgaggtaa tggcctcgac tgatgagcgc gtagtacgga tgcggttcga caacgcatca
       61 ttcatgaagg gcgctgcaga tacgcagaaa tccctcgccg acctcaacaa gggcgtcgac
      121 gctgcaggca acaccaaggg cctgaccaat ctgagcgatc agatgggaca ggtatccatt
      181 tccgcgtcaa agatgcaggt tgctaccgtt gcagccattg gcaccatcgc cgcaaaggcg
      241 acagcactgg gtctcaacgt catcaagggt ctcactttcg agccgattgc ggcagggttc
      301 gccgagtatg aagagaacct gaacaagctc aacaccatca tgaacgcgac gggtaagtct
      361 gagaaggttg tgcagggggt tctcacgaac ctcaacgcat attccgacaa gacgatttac
      421 tcgttcagca acatgaccac ctctatccag aagttcgtga acgcaggcgt tcctctgggt
      481 gaatcggtgg aggccatcaa gggtatcgcc aacgcggctg catattctgg tgccactacc
      541 gaagaggcca accgggcgat gttcgccttc agccagacta tgggtactgg cttcatgatg
      601 ctcaacgact ggcagcagat cgaaaacgcc aacatgggca cggtgaagtt caagcaaacg
      661 ctgatcgaca ccgcagttgc tgccggaacc ctgacgaagc agggtaatgc gtacgtcact
      721 tccactggca agactgtttc cgcgaccaag ggttggcgag agagtctgca ggagcagtgg
      781 gctaccactg aggttgtgac tggtgcgctt tcgaagtaca cagacacaca aaccaagctt
      841 ggtcgtgcgg ccaccgagtc tgcgatggag tacaggacgt tcacggcgtt cttcgactca
      901 ttcaaggaat cgcttggctc cggctgggct aagatcttcg atgcgctgat cggcggtctg
      961 gatgaggcga ctcatttctg gacgtccttc gcgaactctg taacaggcgt tacgggtgct
     1021 ttcttcgagt tcaccgcgaa tgctctcaag acctggcgta ccatgggtgg tttcgagaag
     1081 accattcaag ggttcaagaa ccttctggct cccttgggag ctatatttcg cgttgtgggt
     1141 gctgctttgt cagcagcctt ccccagcggc aacaagggtg tgggcaagac gctctacacc
     1201 atgtccgctg gcttcgaggc aatcacccga ccccttcagt ggttcgcgga cgtcatcgag
     1261 ggcactactc cggtcatcgc gacgttcttc cgaatgcttc atctcatcgg tgcggccatc
     1321 aagtccgctg gcggcacggt tgcgaacttc gtcaaggatc tccttggcat ggtctcgatc
     1381 caggtgccgt ctggtagcgg attcatcgga ttcatcaagg atctggcaaa ggcggtctca
     1441 gatgccagca agaaggttga cgagctcgta cagaagggcg cctcggttgg tgctgccttc
     1501 ggagcggtcg acttcaagat gccgtcaatg ccagacatgc cgagtatgcc caagctctcc
     1561 atgccatcgc tcggtggcgg agacaaggct gcttctggtg tatccacgat gtccgcatcg
     1621 gttggcgatc tcactgggaa tgtcagtcgg ctcaagagcg tgtctgactc cctcaagaac
     1681 agcaccatat tcaactcggc tacgatggcg tccgcaaagg actccttcag gagcgtgggt
     1741 gatggtgcca aggatgccgc tggcggcgta gtttcggctg gagagtggat caagaatgcc
     1801 tgggggtccg ttgtggactt cctcgggccc atcttcagca agatcaagga tttcatcgga
     1861 ggaatctccg cagacgacat cgtctcgtcc ttcaacatgg cgatatttgc gaccatgggt
     1921 atccagctgg cgaagttcat tcacacgctg aacaacatgc tcgacatggt gaacagtttc
     1981 gcgcagatcg ggccagcgtt taccaacgtg ctcgactctg cgggtggtgc gctcaagtct
     2041 ttccagacgc aggcgcaggc caagctg
//
LOCUS       DASTWE010000383         6695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_495515, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000383 DASTWE010000000
VERSION     DASTWE010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495515"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFT30_04060"
     CDS             <1..895
                     /locus_tag="VFT30_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU5091832.1"
                     /translation="FRIDHPSYINVFAHDPELARRMAQRREMFGLKKDGSEFPAEASI
                     SKLSVGERTTFTVIVRDITDRLRTERQLQSLTTELMTAQEEERRRIARELHDDVNQRL
                     ALLAIDMANMLSDSSTLTTHAKETLQSLNQRLVRISDDVRRMAYQFHPSILDDLGLTA
                     ALKHMADEWSEKTGIKTVIVQEEMADPLPRDIASCLYRVAQESLTNIMKHARAARVEL
                     ELTCDDQEITLSIYDTGVGFDLKDIRARHPGLGLVNMRERVRSVRGRLDIQSELGRGT
                     HIIVHIPFSGAPHEETTSSFS"
     gene            867..1511
                     /locus_tag="VFT30_04065"
     CDS             867..1511
                     /locus_tag="VFT30_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5091833.1"
                     /translation="MKKPRVLLADDHTFVLEGFKKLLEEHCDLVGSVEDGRTLIEATL
                     DLQPDLVILDISMPRMNGIEAAKKLKKQIPEVKLIFVTMHADMAYVNAAFRAGASGYL
                     LKRSAATELMQAVQLVMSDKFYVTPLITKEVVASFLKPTQPRLATIDDLTTRQHEILQ
                     LVAEGLSAKEIAEQLKISHRTVEFHKTKIMEQLNVHTTTDLVKYAMAHGLVTAS"
     gene            1838..5275
                     /locus_tag="VFT30_04070"
     CDS             1838..5275
                     /locus_tag="VFT30_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5091834.1"
                     /translation="MVISRRQFLKVSAGTVAAAAVADHVLALTALQPVIEVGNPLGDY
                     PDRSWERVYHDQYRYDSSFTWVCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDLY
                     GNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTPETKRKYK
                     FDSRFLDDMLRVSWDTAFTYAAKAMIVIGTRYSGEAGARRLREQGYAPEMIEMMKGAG
                     VRCFKHRAGMPILGFLGKHSNTRFNNSVLPILDSWIRKVTPDQAQGGRYWNNYTWHGD
                     QDPSQPWWNGTQNCDTDLSDMRFSKMNTSWGKNFVENKMPEAHWKLESIERGCRIVVI
                     TPEYNPTAQRADYWIPLRPQSDASLFLGACKIILDENMQDIDYLKQFTDMPLLVRTDT
                     LQYLDPRDVVSGYKFPDFSHSYSGRIQSLKSDQIERLGGFMVWDLAKKQAVPLHREQV
                     GWHFDKSGIDPALTGSYRVKLLNGREIDALPIYQMYQIHLQDYDLDTVHQITRSPKDL
                     LVRWARDSGTIKPAAIHNGEGVCHYFHMTEMGRAAALVLTLTGNIGKFGTGCHTWSGN
                     YKVGIWNATPWSGVGCGVHLSEDPWRLNLDANAHGKEIKYRNYYYGEEPGYWNHGDTA
                     LIVNTPKYGRKVFTGKTHMPTPSKLRWVVNVNILNNAKHHYDMVRNVDPNIECLMTQD
                     IEMTSDVNHADIAFAANSWMEFTYPEMTATVSNPWIQIWKGGIRPLYDTRNDIDTFAG
                     VAAKLAEMTGDTRMRDVFHFVYQNRMDVYAQRLLDASSTLYGYSADVLLKSEKGWMVM
                     VRTYPRHPLWEETNESKPMWTRSGRIETYRIEPEAIEYGENFVVHREGPEATPYLPNA
                     IFTTNPYVRPDDYGIPITAQHHDDKTIRNIKLSWQEIKRHSNPLWEKGYQFYCVTPKT
                     RHRVHSQWSVNDWVQIYESNFGDPYRMDKRTPGVGEHQVHINPQAAKDRGINDGDYVY
                     IDGNPVDRPYRGWKPSDPYYKVARLMIRAKYNPAYPYHVTMAKHAPFVSTPKSVKGHE
                     TRPDGRAIAIDTGYQSNFRYGAQQSFTRSWLMPMHQTDSLPGKHANGLKFKWGFEIDH
                     HAVNTVPKECLIRITKAEDGGIGARGPWEPVRTGFTPGQENEFMIKWLKGEHIKIKV"
     gene            5370..6659
                     /locus_tag="VFT30_04075"
     CDS             5370..6659
                     /locus_tag="VFT30_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5091835.1"
                     /translation="MPEVYNWQLGRKMLYPYEERHPKWQFAFVFNINRCLACQTCSMA
                     DKSTWLFSKGQEYMWWNNVETKPYGGYPQFYDVKITQLIEQVNPGGQVWNVRVGRKHH
                     APYGVFEGMTIFDAGAKVGQAAIGYIPTDQEWRFVNIYEDTATSMRALVEGIDKTGFS
                     RDEPWKMTGSSLPEHETFFFYLQRICNHCTYPGCLAACPRKAIYKRPEDGIVLIDQNR
                     CRGYKKCVEQCPYKKPMYRGTTRVSEKCIACYPRIEGKDPLTGGEPMETRCMAACVGK
                     IRMQSLVRIGEDGLWAEDRWHPLYYAIRVEQVALPLYPQWGTEPNGFYIPPRHSPRGY
                     ARQMFGPGVDNAIEKYLVPSRELLAVLQLWRASQQIVFRYDVIPGPKVFETQIHAKRF
                     EMYNDTVLGFNKSGKEVARIQVEEPIYIRPAERVTWL"
BASE COUNT         1573 a         1883 c         1935 g         1304 t
ORIGIN      
        1 atttcggatt gatcatccat cttatatcaa cgtgttcgcc catgacccgg aattggctcg
       61 tcggatggca caacgacgtg aaatgttcgg cttgaaaaag gatggaagcg agtttcctgc
      121 agaggcgagc atctcaaagc tgagcgttgg cgagaggacg acgtttacgg tgatcgtgcg
      181 agacatcacg gaccgattga gaacagaacg gcagctccaa tcattaacca ccgaactgat
      241 gacggcgcaa gaagaagaac gccggcgcat tgcacgcgaa ctccacgacg atgtgaatca
      301 gcggctggct ttgctggcga tcgacatggc gaacatgttg tccgattcat cgacgctgac
      361 cacccacgct aaggaaacac tccaatccct caatcaacgg ttggtgagga tttccgatga
      421 tgtccgccgc atggcctacc aattccaccc ttccatcctg gacgacctgg gtctgactgc
      481 ggcgctcaaa cacatggctg atgaatggtc agagaagacg gggattaaaa cagtcattgt
      541 acaagaagag atggccgatc ctttaccgcg cgatattgcc agttgtctct atcgagtggc
      601 gcaggaaagt cttaccaaca tcatgaaaca tgcccgcgcc gcacgtgtgg agctcgaatt
      661 gacctgtgat gaccaagaga ttacgctatc catttatgat accggtgtcg gcttcgatct
      721 caaggacatt cgcgctcgcc atcccgggtt aggtcttgtc aatatgcggg aaagagtgcg
      781 ttccgtgcga ggccgattgg acatccagtc cgaactaggc cgaggcacgc acatcattgt
      841 gcatattcca ttctctggag cgccacatga agaaaccacg agttctttta gctgacgacc
      901 atacctttgt gctcgaggga ttcaagaagt tgctggaaga gcattgcgac ttggtaggat
      961 ccgtagaaga tggtcgcacc ttaattgaag ccactctaga cctccagccg gacctggtaa
     1021 tactggacat ttcgatgcct cggatgaacg gcatcgaggc ggcgaagaag ctcaagaagc
     1081 agatccccga agtcaaactg attttcgtca cgatgcatgc cgatatggcc tatgtcaacg
     1141 cagcgttcag agcaggagcc tcaggctacc tgctgaaacg gtcggcagcc acagagttga
     1201 tgcaggccgt tcagctcgtg atgagcgaca agttctatgt gaccccgctc attacgaaag
     1261 aagttgtggc gtcttttctc aaaccgacac aaccccgttt agccacgatt gacgacctga
     1321 cgacgaggca gcacgagatt ttgcagctgg ttgccgaagg attatccgcg aaagaaatcg
     1381 ccgagcagct gaagatttct caccgtaccg ttgaatttca caagactaag atcatggaac
     1441 aactcaacgt ccatacaacg acggacctgg tcaaatacgc aatggcgcat ggcttagtca
     1501 cggcatcgtg atgtctctgt ttacaacacg gtagctgccc tcactaaaac ctcgtagttt
     1561 ccgcatgcag agccagtaat ctttctttct cccctcccaa taccgctcag ctaaactacg
     1621 ccgtcgtgaa aattggcatg tcaactctga ctcactctga aatcatgctt gtccccaact
     1681 cgtcagtgcg caggcagttg atgaatgaaa gtgaatgcat gttcgttggt caagcgtttg
     1741 acctcataaa atgcgattaa gtcaacaaag accaaggatg ctgatctgtc tcatatctat
     1801 aagtcttcct catattcact aatgggaggt tgagcatatg gttatttcac gtcgccaatt
     1861 tttgaaagtc tccgccggga cggtcgcggc cgccgcggtg gcggaccacg tcctggcctt
     1921 gacggcgctg cagccggtga tcgaagtggg caatccgttg ggggactatc cggaccggtc
     1981 gtgggagcgg gtctatcatg accagtaccg gtacgattcg tcctttacgt gggtctgctc
     2041 gccgaacgac acgcatgcct gccgcatccg ggcctttgtg cgcaacggcg tggtcatgcg
     2101 ggtggagcag aactatgacc accaaacgta tgaagacttg tacgggaacc gggggacgtt
     2161 cgcccacaat ccccggatgt gtctgaaagg gtttaccttc catcggcggg tctatgggcc
     2221 gtatcgcttg aagggtccgt tgatgcgaaa ggggtggaaa cagtggatgg atgacggctc
     2281 gccggagctg acgccggaaa cgaagcgcaa gtacaagttc gacagtcgct tcctggacga
     2341 tatgttgcgg gtctcgtggg atacggcgtt tacctatgcg gcgaaggcga tgatcgtcat
     2401 cggcacgcgg tacagcgggg aagccggcgc ccggcggctg cgggaacaag gctatgcccc
     2461 ggagatgatc gagatgatga agggggcggg ggtgcgctgc ttcaagcacc gcgccggcat
     2521 gcccattctc ggatttcttg gtaaacactc caacacccgg tttaataaca gcgtgttgcc
     2581 gatcctggac agttggatcc gcaaggtcac cccggatcag gcgcaaggcg ggcgctactg
     2641 gaacaattac acctggcacg gcgaccagga cccctcccag ccctggtgga acggcacgca
     2701 aaactgcgat accgatttga gtgacatgcg gttttccaag atgaacacga gttggggcaa
     2761 gaacttcgtg gaaaacaaga tgccggaagc gcactggaag ctcgaatcga tcgagcgcgg
     2821 ctgccgcatc gtggtcatca cgccggagta caacccgacg gcccaacggg ccgactactg
     2881 gattcccctg cgcccgcaat cggacgcgtc gctgttcctc ggggcctgca agatcattct
     2941 cgatgagaac atgcaggaca tcgactatct caagcagttc acggatatgc ccctgttggt
     3001 gcgcaccgat acgctccagt acttggaccc gcgggatgtc gtgtcgggct acaaatttcc
     3061 cgacttttcg cacagctact cgggccgcat ccaatcgttg aaatcggatc agatcgaacg
     3121 gctcgggggc ttcatggtgt gggacctggc caagaaacag gcggtgccgc tccatcggga
     3181 gcaggtcggc tggcatttcg acaagagcgg gatcgatccg gccttgaccg gctcctatcg
     3241 ggtcaagttg ctgaacggcc gcgaaatcga cgcgctgccg atctaccaga tgtatcagat
     3301 ccacctccaa gactacgatc tggataccgt gcatcagatc acccgctcgc ccaaggatct
     3361 cctggtccgc tgggcgcgcg actcgggcac gatcaagcct gccgccatcc acaacgggga
     3421 aggggtctgc cactatttcc atatgacgga aatgggccgg gccgccgccc tcgtgctgac
     3481 cttgacgggg aatatcggga aattcggcac gggctgtcat acgtggtccg gcaattacaa
     3541 agtgggcatt tggaatgcca cgccctggtc gggcgtcggc tgcggcgtgc atctctcgga
     3601 agacccctgg cggctcaatc tggacgccaa tgcccatggg aaggaaatca aataccggaa
     3661 ctactactat ggcgaggagc cggggtattg gaaccacggc gacaccgcct tgatcgtcaa
     3721 tacgccgaag tatggccgca aggtgtttac cggcaagacc catatgccca ccccgagtaa
     3781 gctgcggtgg gtcgtgaacg tcaacatctt gaacaatgcc aagcaccatt acgacatggt
     3841 acggaacgtc gatcccaaca tcgaatgcct catgacccag gacatcgaaa tgacgtccga
     3901 cgtgaaccac gccgacatcg cctttgccgc gaactcctgg atggagttca cctacccgga
     3961 aatgaccgcc accgtctcca atccgtggat ccagatctgg aaaggcggta tccggccatt
     4021 gtacgacacc cgcaacgaca tcgacacgtt tgccggggtg gcggcgaaac tggccgagat
     4081 gacgggggac acccgcatgc gcgacgtctt ccattttgtc tatcagaatc gaatggatgt
     4141 gtacgcccaa cggctcctgg acgcgtccag caccctttac gggtacagtg ccgacgtgct
     4201 gttgaagtcg gaaaagggct ggatggtgat ggtccgcacc tatccgcggc atcccctctg
     4261 ggaagagacc aacgagtcga aaccgatgtg gacccggtcg ggccgcatcg agacgtatcg
     4321 gatcgaaccg gaagccatcg agtacgggga aaacttcgtg gtgcaccgcg aagggccgga
     4381 ggcgaccccc tacttgccga acgccatctt cacgacgaac ccctatgtgc ggccggatga
     4441 ttacggcatt ccgatcacgg cccagcacca cgacgacaag acgattcgga atatcaagtt
     4501 gtcgtggcag gagattaaac ggcacagcaa ccccttgtgg gaaaaaggct accagttcta
     4561 ctgcgtgacc cccaaaacgc ggcaccgcgt ccacagtcag tggtccgtga acgactgggt
     4621 gcagatttac gagtcgaact tcggcgatcc ctaccggatg gacaagcgga cccccggcgt
     4681 cggggagcac caagtccata tcaatccgca agcggcgaag gaccgcggca tcaacgacgg
     4741 ggactatgtg tacatcgatg gcaatccggt ggatcgcccc taccggggct ggaagccgag
     4801 cgatccctac tacaaggtgg cgcggttgat gattcgcgcc aagtacaacc cggcctatcc
     4861 gtaccacgtg acgatggcca agcatgcgcc cttcgtctcg acgccgaagt cggtcaaggg
     4921 ccatgagacc cggccggacg ggcgcgccat cgcgatcgat accgggtatc agtccaactt
     4981 ccggtacggg gcgcaacagt cctttacgcg cagctggctc atgccgatgc accaaaccga
     5041 ctcgctcccg gggaaacatg ccaacgggct caagtttaaa tggggattcg aaatcgacca
     5101 ccatgcagtc aataccgtgc cgaaggaatg cttgatccgc atcacgaagg cggaagacgg
     5161 cggcatcggc gcgcgaggcc cgtgggaacc ggtccggacc ggctttacgc ccgggcaaga
     5221 aaacgagttc atgatcaagt ggctcaaagg cgaacatatt aagattaaag tctaaaagac
     5281 gttaaacgtg aaacgtgaca cgttaaacgc aagacatcgg acgtttaacg aagaacgaag
     5341 aacgattaac gtttaacgag gaggacgcaa tgcctgaagt atataattgg cagctgggcc
     5401 gcaagatgtt gtatccctac gaggagcggc atccgaagtg gcagtttgcc tttgtgttca
     5461 acatcaatcg ctgtttggcc tgtcagacct gtagcatggc cgataagtcg acctggctct
     5521 tctccaaagg gcaggaatac atgtggtgga acaacgtgga aaccaagccg tacggcgggt
     5581 atccccagtt ctacgacgtc aaaatcacgc agctgatcga acaggtcaat ccggggggcc
     5641 aggtctggaa cgtccgcgtc ggccggaaac accatgcccc ctacggcgtg tttgaaggga
     5701 tgaccatttt cgatgccggg gccaaagtag gccaggccgc catcggctat attcccacgg
     5761 accaggaatg gcggttcgtc aacatctacg aagacacggc cacctcgatg cgcgccctgg
     5821 tcgagggcat cgacaagacc ggcttctcgc gggatgaacc ctggaagatg accggcagca
     5881 gcctcccgga acatgagacc ttcttcttct atctccaacg catttgcaat cactgcacct
     5941 atccgggctg cttggcggcc tgtccccgca aggccattta caagcggccg gaagacggca
     6001 tcgtcctgat cgatcagaac cgctgccggg ggtacaagaa atgtgtggag caatgtccgt
     6061 ataagaaacc catgtaccga ggcacgaccc gggtcagcga gaaatgtatc gcctgttatc
     6121 cccgcatcga agggaaggat ccgctgaccg gcggtgaacc gatggaaacg cgctgcatgg
     6181 cggcctgcgt gggcaagatc cgcatgcaga gcctcgtgcg gatcggcgag gatggcctgt
     6241 gggccgaaga tcgctggcat ccgctgtact atgcgatccg ggtggagcaa gtggccttgc
     6301 cgctctatcc gcaatggggg accgagccca acgggttcta catccccccg cggcattccc
     6361 cgcgcggcta tgcccggcag atgttcgggc ccggcgtgga caatgccatt gaaaagtatc
     6421 tggtgcccag tcgggaactg ttggcggtgc tgcagctctg gcgcgccagc cagcagatcg
     6481 tgttccgcta cgatgtcatt ccgggcccga aggtcttcga aacccagatc cacgccaagc
     6541 ggttcgagat gtacaacgac accgtgttgg gcttcaacaa gtcgggaaag gaagtggcgc
     6601 ggattcaagt cgaggagccg atctacatcc ggccggcgga gcgcgtcacc tggttgtaat
     6661 ctggtacaga cactgtgtta gcagggggcg gaatc
//
LOCUS       DASTWE010000384         1391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1432750, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000384 DASTWE010000000
VERSION     DASTWE010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432750"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..839)
                     /locus_tag="VFT30_04080"
     CDS             complement(144..839)
                     /locus_tag="VFT30_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5091836.1"
                     /translation="MPSALITGANRGLGLEFSRQYFADGWRVYAACRDPASASELRRL
                     AEGSADKLRILAMEVTDPASIHAAATQLDGQAIDLLLNNTGIIGPRGQTIGNIDYEAW
                     ANVLAVNTMGPMRVSEAFVEHVARSERKLIVTLTSGMGSIADNTSGGSILYRSSKAAV
                     NMVMRSLAIDLAPRGITCVVINPGWVRTDMGGMNATLDPSESISRMRRVIATLGPELS
                     GKFFNHTGREYPW"
BASE COUNT          289 a          399 c          386 g          317 t
ORIGIN      
        1 ttcccccgac atggacgcta atccgttggt ctccgactaa gaccattcgg ttcttgaagc
       61 ctggaaagat cgactctgcc atcagcctgc tcaggtagaa tggcccagcg cctgacggtt
      121 actcgctacg caccgtatgc aggctaccag ggatattcgc gaccggtgtg attgaagaac
      181 ttgccggaca gctcggggcc gagcgtcgcg atgacacgtc tcatccggct tatgctttcg
      241 gacgggtcta aggtggcgtt catcccaccc atatcggtcc ggacccagcc tggattgatg
      301 acgacgcatg tgatgccgcg aggtgcgagg tcgattgcca gactgcgcat gaccatattc
      361 actgcggctt tcgagctgcg atagaggatc gagcctccag acgtattgtc ggcaatggac
      421 cccatgccgc tagtaagcgt gacgatgagc tttcgctcgc tgcgtgccac gtgctcgaca
      481 aatgcttccg agactcgcat cggacccatg gtgttgaccg cgagcacgtt cgcccaagct
      541 tcgtaatcaa tgttgccgat tgtctggcct cgtggtccga taatgccggt gttattgagc
      601 agaaggtcga tggcctgacc gtcaagttga gttgccgccg catggatgct ggctgggtca
      661 gtgacttcca tggcaaggat ccgcaatttg tcagcgctgc cctcagcgag tcggcgcagc
      721 tcggaagcag atgctggatc acgacaagct gcgtagaccc gccatccgtc agcgaaatac
      781 tgacgagaga actcaagccc tagtcctcga ttggcacccg tgattagcgc actcggcatt
      841 ggtccttctc cattgcgatg catcggttcg acgaagtccg agttcaccta attcatttca
      901 cgcatgatac gttcgaagaa gccgctcttg tcgccgacct aaccggaccg ccaaattgcc
      961 gaacggagaa ttttgaaccg aggggccggc gcaattctcc gaactgcgct ctctacggcc
     1021 gcttaagcca gctctggtga cgctaaccta cggatattcc gcgagaattg cgcggcacga
     1081 aatttatttg caaaaacaac ggactggctg gcggagcgag caggattcga actcccggtc
     1141 cggtcaggaa aattggcctt tgaattttcg aatcgccctt cactcgccaa attcggcttc
     1201 agagaaaatt tcccgcctga ggtgctggac gggatttttg cctagtctcc gcggtccggt
     1261 tgccgttatg gagttagcgg ggatctccgg ttccaactag atcgcgatcc tagttccagt
     1321 gagtcgcctc caagtctgaa gcggattagc tcttcagcaa atcgtcgtat cgcggctcca
     1381 ttgcatgccc a
//
LOCUS       DASTWE010000385         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_947861, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000385 DASTWE010000000
VERSION     DASTWE010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947861"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..>1341
                     /locus_tag="VFT30_04085"
     CDS             133..>1341
                     /locus_tag="VFT30_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5091837.1"
                     /translation="MIKADNEKEIFLRSFKKLENQVQILPVSETQIQRLQSLLSGIQK
                     TAENLALYDPLTHALNTRAGEWLIPKEQITGLAKIDIYDLRQANKVYGVAVVDSELHR
                     LAYQLMSIFTMDKGDFVCRSPGSDEFRILSTSKTPQEVRLLLTKPYLDQESDSLLTWD
                     FGTGRTETEAENNLQKQRKLFRPLVIRQTILESHSEIPRRLEGNNSHLSWHEFNMPYK
                     RLVDTICSSELPEELQREATKQVHVIRSMVEGIVTKEALTGTLNAIGAKWYLEQAAVQ
                     SVALTDMLDMHEGNARYGSVAIDQDLKRFAKLLLRHFPKKEGFLLFRSERAGDEFKIV
                     STKSGATELESRIRMVWQNDLGRGLLAWNYGVGSDDAEAHVDLYRNRVKEIESMEVSV
                     SNGKSTFIIVR"
BASE COUNT          373 a          331 c          355 g          282 t
ORIGIN      
        1 gaaagccgct ttggcgctga tcaatgaatc cattgaaagc ttccaacagc aaggcatgca
       61 atacgaaatt caaaaggcga aagtgctgca ggaggaagtc ctgaaagcac atcctcaaat
      121 actcacatcg caatgataaa agccgataac gaaaaagaga ttttcctgcg ttccttcaag
      181 aagctggaaa atcaagttca aatcctgcca gtctctgaaa ctcaaatcca gcggctgcaa
      241 agtctgctat cggggatcca gaagactgcc gaaaatctgg cactctatga tcctttgacg
      301 catgccctga acacccgtgc gggagagtgg ctgatcccaa aggagcagat tacagggctc
      361 gccaagatcg acatctatga tctgcggcag gcaaataaag tctatggcgt ggcagtagtc
      421 gattcagagc tgcataggct agcctatcag ctcatgtcca tattcaccat ggataagggc
      481 gactttgtct gccgttcgcc gggcagtgac gagttccgga tcctgtccac ctcgaaaacg
      541 ccgcaggagg tccggctgct gctgaccaaa ccgtatcttg atcaggaaag tgattcgtta
      601 ttgacctggg atttcggtac aggacgcaca gagaccgagg ccgagaacaa cctccagaag
      661 cagcgcaagc tgttccgccc gcttgtcatc aggcagacga ttctggaaag tcactccgag
      721 attccccgac ggctggaggg aaataacagc catctgagtt ggcatgagtt taatatgcca
      781 tacaaacgac ttgtcgatac aatttgttcg tcagagttgc cagaagaatt acagcgagag
      841 gcaaccaagc aggtccatgt cattcgatct atggtggaag gcatcgtcac caaggaggca
      901 ttgacaggta ccctcaacgc tattggggca aagtggtacc tcgagcaggc ggctgtccaa
      961 agtgtcgccc tcaccgacat gctggatatg cacgaaggca atgctcgcta tggaagtgtg
     1021 gcgatcgacc aggatctcaa acgctttgcg aagctccttc tacggcactt cccgaagaag
     1081 gaaggattcc tgctctttcg atcggaacgc gccggtgatg aattcaagat tgtatcgacc
     1141 aaaagcgggg cgacagaact ggaaagcagg atccgcatgg tctggcagaa cgacctgggt
     1201 cgtgggctgc tcgcatggaa ttacggtgtt ggcagcgatg acgccgaagc tcatgtcgac
     1261 ttatacagga acagagtaaa ggaaattgaa agtatggaag tatctgtttc caacggcaaa
     1321 tcaaccttta tcatcgtccg g
//
LOCUS       DASTWE010000386         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_818751, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000386 DASTWE010000000
VERSION     DASTWE010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818751"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFT30_04090"
     CDS             <1..696
                     /locus_tag="VFT30_04090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5091838.1"
                     /translation="NEARLIERSLQSVAVSIADNQTSGFTSEIIVVDNNSTDNTAELA
                     RQAGAKVVFEPINQIGRARNAGAAYATGDWLLFLDADSLLSPELLADVVRVIESGKYV
                     GCGSTLRMDGLPWWANMTLLLWTGASVLFRWAAGALIVCRRDAFQEVGGFDQDLYALD
                     EIRLSKQLKQWGRRLGLQFTILTGHPLETSSRKVLLYSGREIAAQIFRIFVLPKKTLQ
                     DKKHLSVWYDGRR"
     gene            complement(733..993)
                     /locus_tag="VFT30_04095"
     CDS             complement(733..993)
                     /locus_tag="VFT30_04095"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEU5091839.1"
                     /translation="MARTKPEFKVGDHVRWNSEAGRVSGTIKQKITSPRKFKNYTVRA
                     SKEEPQYLIKSDKTDHLAMHKGAALKKIFEKSHTPKKRQYRA"
     gene            complement(1003..>2014)
                     /locus_tag="VFT30_04100"
     CDS             complement(1003..>2014)
                     /locus_tag="VFT30_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5091840.1"
                     /translation="SIWRDTKKREVTTHLGGIVREVVADQKRVLCEDGTAFTYEKLLL
                     ATGGMPRRLPFGGDHIIYYRTLFDYRRLRALTETGRRFAVIGGGFIGSEIAAALAMNG
                     KEVVMIFPDSDIGVRIFPLPLARFVSDFYRSKGVEVLAGQQPIGVERREGKYILTTSA
                     KQDISVDGLIAGIGIEPNVGLARAIGLRIEDGIIVDELLRTSRPDIYAAGDAVSFYNP
                     ALGTHLRVEHEDNANSMGRLAGRNMAGKSEPYHHLPFFYSDLFDLGYEAVGQTDSRLV
                     TFADWKRPNEEGVIYYLENGRVRGVLLWNVWEQVEAARRLIAEPGPFNAENLKGRIAA
                     KV"
BASE COUNT          424 a          570 c          521 g          499 t
ORIGIN      
        1 aacgaagccc gtctgatcga acgatctctg caatccgtcg ctgtatcgat cgctgacaac
       61 cagacatccg gtttcacatc cgaaatcatt gttgtcgaca ataactccac tgacaacacg
      121 gcggagttgg ctcgacaggc cggagcaaaa gtcgtctttg aaccgatcaa tcaaattggc
      181 cgggcacgca atgcgggagc agcttatgcc accggcgatt ggttgctgtt cctggatgca
      241 gatagtctgc tgagtccgga gctgttggcg gatgttgtac gagtgatcga gtcgggaaag
      301 tacgtggggt gcggcagcac gttgcgcatg gatgggttgc cctggtgggc caacatgaca
      361 ttgctgctct ggaccggcgc ctcggttctt tttcgctggg ctgccggggc gctgatcgtt
      421 tgccgtcgcg atgcatttca ggaggtcggc gggttcgacc aggatctcta cgcattagac
      481 gaaattcggc tcagcaaaca actcaagcaa tgggggcgac gactcggcct tcaattcacc
      541 attttgacgg ggcatccgct cgaaacgtca tcccggaagg tcttgctcta ctcaggccgg
      601 gaaattgctg ctcagatctt ccgaatcttt gttcttccga aaaagacatt acaggataaa
      661 aagcacctct ccgtgtggta tgacggacga cgctgacttg aaccgttcgt cgatttaacc
      721 atcgcgatcg ttttaagctc tgtactgccg tttcttcggt gtgtgtgatt tttcgaagat
      781 ttttttgagc gccgcgcctt tgtgcatcgc taggtgatcg gtcttatcgc tcttgatgag
      841 atattgcggt tcttccttcg aagcgcggac cgtataattc ttgaacttcc tgggagaagt
      901 aattttctgc ttgattgttc cgctgacacg tcccgcttcc gagttccacc tcacatgatc
      961 cccgacctta aattctggtt tcgtccgtgc catgtctaaa tttcaaacct tggccgcgat
     1021 ccgccctttg agattctcag cgttgaacgg accgggttca gcgatcaacc ggcgagcagc
     1081 ttcgacttgc tcccatacat tccataacaa caccccgcgt actcgaccgt tctccagata
     1141 gtagatgacc ccttcctcgt ttggtcgttt ccaatcggcg aacgtcacca accgtgaatc
     1201 cgtttgtccc acagcctcat accccaagtc gaacagatcg gagtaaaaga acgggaggtg
     1261 gtgatacggc tcggacttgc ctgccatatt ccgccctgcc aggcgtccca tggaattggc
     1321 gttgtcctcg tgctcaactc gaaggtgtgt ccccaacgca gggttgtaaa acgataccgc
     1381 atcacctgcg gcatagatat cagggcgact cgtccgcaac agctcgtcca cgatgatgcc
     1441 gtcttctatt ctgagtccga tggctctggc gaggccaaca ttgggctcaa tgccgattcc
     1501 tgcaattagc ccatcgacgc tgatgtcctg cttggcgctt gtcgtcagga tatacttccc
     1561 ctcccgcctc tccactccga ttggctgttg acccgccaaa acttcaacac ctttgcttcg
     1621 gtaaaaatcg gacacgaacc gggccagcgg cagcggaaag atacgaacgc cgatatcgct
     1681 gtcgggaaag atcatgacga cctctttccc gttcatggct agcgccgcgg cgatttcgga
     1741 gccgatgaat cctccgccga tgacggcaaa gcgccgacct gtttcggtga gcgcgcgtag
     1801 tcggcgataa tcaaagagtg tccgatagta gatgatatgg tctccaccga agggcaagcg
     1861 tcgaggcata ccaccggtag ctaataagag cttttcatag gtgaaagcgg ttccgtcctc
     1921 gcataagacg cgcttctggt ccgcaacaac ttctcggaca attcccccaa gatgcgtcgt
     1981 cacctctctt ttctttgtgt cccgccagat actg
//
LOCUS       DASTWE010000387         1854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1109727, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000387 DASTWE010000000
VERSION     DASTWE010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109727"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..371)
                     /locus_tag="VFT30_04105"
     CDS             complement(171..371)
                     /locus_tag="VFT30_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091841.1"
                     /translation="MNLEDARKRIESAVTQYGQHAAPAIDLVMAEVRSDLGAGAFNEL
                     IEEFDLELMYNITPMESDHSSS"
     gene            complement(404..718)
                     /locus_tag="VFT30_04110"
     CDS             complement(404..718)
                     /locus_tag="VFT30_04110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5091842.1"
                     /translation="MDINAFRQMVAKNPKGFLGRYGLGNKILQENGSVEEAVEHLTVA
                     TQLDPTHAASHLVLGRALIRLGRNADAKPVLTAGIEAVLSGRSNGGKDLVPEMQELLR
                     SL"
     gene            complement(810..1562)
                     /locus_tag="VFT30_04115"
     CDS             complement(810..1562)
                     /locus_tag="VFT30_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091843.1"
                     /translation="MTMNMLRWSALSLAGFVLCGITGCTRWIDVKPPSADPKETISHS
                     LPTDERVPLVTNSFHLSRNGASQYPSIDLERRILSSIQETNLFSTLLPLGEKSDSLGD
                     KVVSARITVDETIEPHSWLSAFKGIIIGGSMFLLSPFIDLEYGYAATVALELERWDGQ
                     VTRYEGRSSGSVHYKLFGATPVMIDELKGHVTEACLTDLTGQLVRDTKLYMASSAPLP
                     DTGIRMVTVKAKRPAAIQSSRSLVPVATATTP"
     gene            complement(1576..>1854)
                     /locus_tag="VFT30_04120"
     CDS             complement(1576..>1854)
                     /locus_tag="VFT30_04120"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU5091844.1"
                     /translation="RIKYTFVALGRIRPYLEFAGGPFWTDLGGRIREQANEFNFVLTG
                     GVGVSWFLTPQLAFNVGYRFHHISNAGTAFPNLGLNASLPFAGFSFYF"
BASE COUNT          440 a          474 c          535 g          405 t
ORIGIN      
        1 tacgcaggag gacgcgaagg tcagggacga gacgatgtaa cgcattgagg accggcacga
       61 cttgcgccgc atgaccaaat ccatgtcctg agattgcagc ccagatcagc ggcataggcg
      121 gaaggatagt gtgagagtca cgagtgaacg gtggagtaag aagacggtat tcaactacta
      181 gagtggtctg attccatcgg agtaatgttg tacatcaact caagatcaaa ctcttcaatc
      241 agttcattga atgcgcccgc tcccaaatcg gaccgaactt ctgccatgac gaggtcgatg
      301 gctggggcgg cgtgttggcc gtattgcgtg acggctgatt caattcgttt cctggcatct
      361 tcgaggttca cggcgagact cctttatggt agaggctcat tcatcacagt gagcgtagca
      421 actcctgcat ctcaggcacc aggtccttgc cgccgttgga tcgtccagat agtaccgcct
      481 cgataccggc agtaagaaca ggtttggcgt cggcgttcct tcctaatcgg attaaggcac
      541 gtcctaacac cagatgagag gctgcatgcg tcgggtccaa ttgtgtggcg acagtgagat
      601 gctcgacagc ttcttcgacg ctgccgtttt cttgaaggat cttattgccg agtccatagc
      661 ggccgaggaa tcccttggga ttcttggcga ccatctgacg aaaagcatta atgtccatcg
      721 atcatggctc cagtgcctaa gcggtactat accactggaa cggcaggatc ggccagatga
      781 gaatgttggt tggagggact ccggtcttac taaggtgtcg tggccgtcgc gaccggaacg
      841 agggaacgtg aggattgtat ggccgcgggc cttttggctt tcacagttac catgcgaatg
      901 ccggtgtctg ggagtggggc actgctggcc atgtaaagtt tggtatcgcg aacaagttgt
      961 cccgtcaggt cggtgagaca ggcttccgtc acgtgtccct tgagctcatc gatcatgacc
     1021 ggcgtggcgc cgaacaactt ataatgcacc gaaccggagg aacggccctc gtagcgcgtg
     1081 acttggccgt cccaacgttc gagctcaagg gcgaccgttg cggcataccc atattccagg
     1141 tcgatgaagg gcgagaggag aaacatggat ccaccgatga tgatgccttt gaatgccgag
     1201 agccaggaat ggggctcgat ggtttcgtcg acggtgatgc gcgcagagac gaccttgtct
     1261 ccgagtgaat cggacttttc tcccagcggc agcaatgtcg aaaacaggtt ggtttcctgt
     1321 atgctgctta agatccgtcg ctcgaggtca atagagggat attgcgacgc tccgtttctt
     1381 gagaggtgaa acgaattcgt cacgagcgga acccgctcat ccgtggggag cgaatgcgaa
     1441 atggtctctt tgggatcggc tgagggcggt ttgacatcga tccatctagt acatcccgtt
     1501 atgccgcaca gcacgaaccc agcaagactc agagccgacc atcgcaacat gttcatcgtc
     1561 atgatcgccg cctcctcaga aatagaatga aaatcctgca aagggcaaac tcgcattcag
     1621 cccaagattc ggaaaggctg taccggcgtt ggaaatatga tgaaaacgat aacccacatt
     1681 gaaggcgagt tgcggcgtaa gaaaccacga tacgcccaca cctcccgtga ggacgaagtt
     1741 gaactcattg gcttgctcac gaatacgacc accgagatcc gtccagaacg gcccgcctgc
     1801 gaattccagg tatgggcgga tgcgacccaa ggcaacaaac gtgtacttga ttct
//
LOCUS       DASTWE010000388         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1303527, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000388 DASTWE010000000
VERSION     DASTWE010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303527"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VFT30_04125"
     CDS             <1..1585
                     /locus_tag="VFT30_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5091845.1"
                     /translation="EGVAQWEFRKVRSDGAVLWVRELARVLQSRDGESLALIVCEDIT
                     ERKHSEEALRASEERFAKAFQASPHPVVISELDSGRVVDANDAAYRMFGYRREEVEGR
                     TTLQMGLWHSAEERARFVERLKRVGSIRNMEVTLRSGDGEIRQCLLSSEPIELNGKQC
                     SVTVGDDITDRKRAEAELHRSHTFLRQVIDTDPDLIFAKDRDGRFTMANKAVADWYGT
                     TVENLIGKSDADFNANVEEVECFRQSDLEVIHSARDRFIPEEKITDAGGRMRWLQAVK
                     RPIFDDEGRVHMVLGAATDITERKRMEEILLQRERDLSAALQERERISQDLHDGILQS
                     LYAVGLGLEACKPLIKNQQDHTVEKFVATLNQAIGQLNQVMAEVRNFIAGLESQVMQG
                     GDFSTALRTMVETMSLSSSARCRVRIDDAAAQRLSTEQALHIINIVREGLSNALRHSR
                     AKQITVSLRDLIRSDRLAVTDDGVGFNARSVQGVGHGLVNMAARAQKVRGLFAIQSKP
                     DRGTRISLDLPKDTHYAHH"
     gene            1572..2228
                     /locus_tag="VFT30_04130"
     CDS             1572..2228
                     /locus_tag="VFT30_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5091846.1"
                     /translation="MLTTKAQAIRVLLVDDHEVVRVGLRTVLGQTQGVTVVGEASTMA
                     DAIQQAQRLKPDVILMDVRLPDGSGVDACREILGTLPGTRVIFLTSYADDDSVLAAVL
                     AGAHGYILKEIDSSALLEAILSVAKGQSILDSSVTERALRWLRGLHDLPATPGTDQLS
                     SQEERVVALVAEGKTNKEIAVALGLSDKTVKNYLANVFQKLRITRRAQAAAFFVKRQG
                     "
     gene            2385..>2716
                     /locus_tag="VFT30_04135"
     CDS             2385..>2716
                     /locus_tag="VFT30_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5091847.1"
                     /translation="MTAGLFLGLLLSLSFLLWVLIRQRQENVLNSTVIAATNCSVLVT
                     DATLPNHPIVYVNPAFLLLTGYVEQEVIGQTTTILTGQDTDRASMEKLAMALQDGWAC
                     RVRLCHYR"
BASE COUNT          628 a          694 c          806 g          588 t
ORIGIN      
        1 ggaaggagtt gcgcaatggg aatttcgcaa ggtgcgaagc gatggggcgg tgctgtgggt
       61 acgcgagctt gcacgtgtgc tgcaaagccg ggacggagaa tcattggcac tgatcgtatg
      121 cgaggacatc accgaacgca agcactcgga agaggctctg cgggcaagcg aagagcggtt
      181 tgccaaagcg ttccaagcta gtccccatcc ggtcgtcatc agcgagttgg attcaggccg
      241 ggtagtggac gcgaatgatg ccgcataccg gatgttcgga tatcgcaggg aagaagttga
      301 gggacggacc acgttacaga tgggactctg gcattcggcg gaggaacggg ctcgcttcgt
      361 cgaacggttg aagcgtgtcg ggtcaatccg gaacatggag gtcactctgc ggagcgggga
      421 cggcgaaatc cgccagtgcc tattatcatc ggaaccgatc gagctgaatg gcaagcagtg
      481 cagtgtgacg gtcggagacg atatcaccga ccgcaaacgg gcggaagctg aacttcaccg
      541 ctcccatacg ttcttgcgac aggtcattga cacagacccc gacctcattt ttgccaagga
      601 tcgtgacgga cgttttacca tggccaacaa ggctgtggcc gattggtacg gcactaccgt
      661 ggaaaattta atcggaaaat cggatgccga cttcaacgcc aatgtggaag aggtggaatg
      721 ttttcggcag agcgatcttg aggtaattca ttccgcgagg gatcggttca tcccggagga
      781 gaaaatcacc gatgcagggg gaaggatgcg ctggttacag gcggtcaaga ggccgatttt
      841 tgatgacgag ggccgggtcc atatggtgct cggagccgcg accgatatca ccgagcgtaa
      901 acgaatggag gagatactcc tgcagcgcga gcgagatttg agcgcggcgc ttcaagaacg
      961 ggaacggatc agtcaagacc tccatgacgg tattctccag tccttgtacg ccgtcgggct
     1021 agggttagaa gcatgtaaac cactgatcaa gaaccaacaa gatcatacgg tggagaaatt
     1081 cgtggcgaca ctgaatcaag ccatcgggca gctcaaccaa gtcatggcgg aggtccgaaa
     1141 tttcattgcc ggactcgaat ctcaagtaat gcagggtgga gacttctcga ccgctctgcg
     1201 gaccatggtc gagacgatgt ccctctcctc ttctgccaga tgccgggtga ggatcgacga
     1261 tgcggcggca cagcggcttt ccaccgaaca ggcgctccat attatcaata tcgtacggga
     1321 agggttgagt aatgcactgc gccacagccg cgccaaacag atcactgtat cgcttcgaga
     1381 tctcatccgt tcagatcgtc tcgccgttac ggatgacggc gttgggttta atgcacgctc
     1441 ggttcaggga gtgggtcatg ggttagtcaa catggcggca cgagctcaaa aagtccgcgg
     1501 cttgttcgcc atccagtcga aacctgacag ggggacgagg atctcactcg atcttccaaa
     1561 ggatactcat tatgctcacc actaaagctc aagccattcg agtactgctc gtcgacgatc
     1621 atgaggtcgt tcgggtggga ttgcggacgg tgctcggcca aacccaaggt gtcaccgttg
     1681 taggggaggc gagtacgatg gcggatgcga tccagcaagc gcagaggttg aagcccgacg
     1741 tgattttgat ggatgtccga ctgccggacg gatccggtgt cgatgcctgt cgggagatcc
     1801 tagggacgtt gccgggaaca cgggtcattt tcctgacgtc ttacgccgac gatgattccg
     1861 ttctcgccgc ggtgctcgcc ggcgctcatg gctatatcct taaagaaatc gattcatctg
     1921 cattgcttga ggccatcctt tcggttgcga aaggccaatc gattttagac tccagtgtga
     1981 cagaacgggc tctgcggtgg ttgagagggc ttcacgatct acctgcgacc cctggtacag
     2041 accaactgtc ctctcaggaa gaacgagtag tcgcactggt tgccgaggga aagaccaata
     2101 aggaaatcgc cgtcgcactc gggcttagcg acaagaccgt caagaattac ttggcaaacg
     2161 tatttcaaaa gcttcggatc acacggcgcg cgcaggcggc cgcctttttt gtgaaacggc
     2221 aaggataagc gatgaacatt cggctctagt aaatttaggt gcaaaaatct agctttgcag
     2281 ctacccttca tacctgggca ttccccttgc gcttcatgta gattgacctc acgttattcg
     2341 tattcttaac tactccgtgt tcgcacacca ggaggttgtc ccgcatgact gctggtctct
     2401 tcctagggtt gctcctgtcc ctcagtttct tgctatgggt attgattcga cagcgccaag
     2461 agaacgtcct gaattcaacc gtcatcgcag cgaccaattg tagcgtgctc gtgacggatg
     2521 ccacgttgcc caatcatccc atcgtctatg taaatccggc cttcctgttg ctgaccggct
     2581 acgtcgagca ggaggtgatc ggtcaaacga cgacgatctt gaccggccag gatacagatc
     2641 gggcctcgat ggaaaaactt gcgatggctc tccaagatgg atgggcctgt cgtgtgcggt
     2701 tgtgccatta ccgcaa
//
LOCUS       DASTWE010000389         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_366155, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000389 DASTWE010000000
VERSION     DASTWE010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366155"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFT30_04140"
     CDS             <1..705
                     /locus_tag="VFT30_04140"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU5091848.1"
                     /translation="DAGLNRVTVSLDTLRPERFRRLTGRDEFARVLEGIESVGKTGFT
                     NLKLDTVAIRGFNEDELSPLIEFAKHYQAEVRFIEYMDVGGANEWRMDKVLSQDMILD
                     ALARRYGRITALPERGAAPAQRFCLPDGTIFGIIPSTTMPFCAQCDRSRLTADGLWYL
                     CLYAGSGVDLRKPLRMNERPEQMREIIRSGWVARHDRGAEERKALERVGLREGGLIDI
                     DRLREDPHLEMHARGG"
     gene            810..1517
                     /locus_tag="VFT30_04145"
     CDS             810..1517
                     /locus_tag="VFT30_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5091849.1"
                     /translation="MPETQFLTLLGVGFLLGLRHALDTDHLAAVSTVLAERPSLLASS
                     VVGLCWGVGHTLTLLFVGSIVLVSGLHISDQFELIAESGVGILLIVLGTSLALKLYRE
                     RWHVHSHRHDGAPHVHFHSHQRQDDHGHRHWMANSIRPLCIGMAHGLAGSAALMLMIL
                     TTTTGVAAGILSIFIFGLGSIVGMMVIGMTITIPVIWSRSISRRLFVGVQGFASLASV
                     LVGVWMLVKLVPSVIGH"
     gene            1640..>2015
                     /locus_tag="VFT30_04150"
     CDS             1640..>2015
                     /locus_tag="VFT30_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HEU5091850.1"
                     /translation="MAWFKKEKPAESVPPPRSKGSEGMWLKCNHCREIVYRKEVDRNS
                     KVCPKCEYHFPISVTERIGLLIDLGTFKEWDADLEAQDPLTFQDTKPYRDRLKAHQEK
                     TGRKDALVIGEGLVNGRRVVLCV"
BASE COUNT          420 a          497 c          600 g          498 t
ORIGIN      
        1 gatgcggggc tcaaccgagt cacggtcagc ttagatactc tgaggccgga gcgatttcgc
       61 cgactgaccg ggcgggatga gtttgcacga gttctggaag ggattgaatc agtcgggaaa
      121 acaggcttca ccaacttgaa gctcgatacc gtcgccattc gtgggttcaa tgaggatgag
      181 ttgagcccat tgatcgagtt tgcaaaacac tatcaggccg aagtccggtt catcgaatat
      241 atggacgttg gtggagcgaa tgaatggagg atggacaaag tcctctccca ggacatgatc
      301 ctcgatgccc tcgctcgacg gtatggccga atcacagccc ttccggaacg gggggcagct
      361 cccgcccaac ggttttgctt gccggatggc acgatctttg gaattattcc atcaacgaca
      421 atgccgtttt gtgcccagtg cgaccgcagt cgtctcacgg cggacggctt gtggtatctg
      481 tgtttgtatg cgggatcggg agttgacctc cgcaagcctc ttcgcatgaa cgagcgcccc
      541 gagcaaatgc gcgaaatcat tcgatcgggt tgggttgctc gccacgatcg tggagcggag
      601 gagcgtaagg cgttggaacg agtagggctt cgagaggggg ggctaatcga cattgatcgg
      661 ctgcgagaag atccccatct ggaaatgcat gcccgtggtg gataagtgcc tggagctgca
      721 tcctatttgg ctcttgactc ggtgcttaga tatgctattg gtttcgcttg cgggatcgtc
      781 gttcgatgcc tctagtttcc tgaagcatca tgcctgaaac tcaattcttg acattgttgg
      841 gagtcggttt cttgcttgga ctacgacatg ccctggatac ggatcatctt gcagcagtct
      901 caacggtgct tgccgaacgg ccgtctcttc tggcctccag tgtcgtcgga ctctgttggg
      961 gcgtcggaca tacactcacg ctgctgttcg tcggatcgat tgttctcgtt tccgggcttc
     1021 atatctctga tcaatttgaa ctcattgctg aatcgggtgt agggattctc ctgattgtcc
     1081 tgggcacttc gttggcactc aaactatacc gtgagcggtg gcatgtgcac agccatcgcc
     1141 atgacggagc gccgcatgtc cacttccaca gtcatcaacg acaagacgat catgggcatc
     1201 ggcattggat ggccaattcg attcgcccgc tttgtatcgg catggcgcac ggattggctg
     1261 gatcagcggc gctcatgctg atgattctta caacgacaac gggcgtggct gccgggattt
     1321 tgtcgatttt catcttcggc cttgggtcaa ttgtcggcat gatggtaatc ggcatgacga
     1381 ttaccattcc tgtgatttgg tcgcgttcca tcagtcggcg actgtttgtg ggagtacaag
     1441 gttttgccag tcttgccagt gtcttggtcg gggtgtggat gttagtgaag ctggtccctt
     1501 cagttattgg acactaaggt ggtccggaat tcagtgggat gcgtagggaa gggtgcatcg
     1561 ggggtgggcc tatgctaatc tctgccgatt tgtgtagaac aatgcatcga taggataagg
     1621 tttcataaag gcgacgacca tggcctggtt caagaaagaa aagcccgcag aatctgtccc
     1681 gccgccacgt tctaaaggca gcgaggggat gtggctcaag tgcaaccact gtcgggagat
     1741 cgtctatcgg aaggaagtcg accggaacag caaagtgtgc ccgaagtgtg aatatcactt
     1801 cccgatctcg gtcaccgagc gaatcgggtt gctgatcgac cttggtacct tcaaagaatg
     1861 ggatgccgac ctcgaagccc aagatccctt gaccttccaa gacaccaagc cctatcgaga
     1921 tcgcctgaaa gcccaccaag aaaagacggg tcggaaggat gctctcgtca ttggcgaagg
     1981 cctggtcaac gggcgccgtg tggtgctctg tgttt
//
LOCUS       DASTWE010000390         1311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1174207, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000390 DASTWE010000000
VERSION     DASTWE010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174207"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..>1311
                     /locus_tag="VFT30_04155"
     CDS             82..>1311
                     /locus_tag="VFT30_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5091851.1"
                     /translation="MNNFVQIALKKPYTFVVLAILILVFGALTVVEAPTDVFPNIQMP
                     VSSVVWLYDGLMPQETEGRVTRVFEQMLTQTVEGIKTIVSTSYFGHSIINIFLQDGVD
                     LAGSEADIVGISQTSVKALPPDVAAPMVMRLFPSQVPVAVLQVESDVQTPAELYNLCV
                     MRIRPMLVTIPGAILPHPYGGQDMQVMINVDQQKLLARHLTTEDVHQAIERQNLVLPG
                     GDMKIKETDWLVLTNASPLKIEEFDDIPIKREGNAFIHLRDVATVRLAGRVQTNSVLV
                     DGKQAVIVVVMKSSEASTIDIVDGIKKMLPRIQEVVPPDVKVSLISDSSIFVKDAISD
                     VVHEMVIAAALVGFIVLLMLGSWRPTVIVLTSIPLSILSSIICLHLLEESINIMTLGG
                     LALAVGILVDNAAVMIEN"
BASE COUNT          309 a          372 c          327 g          303 t
ORIGIN      
        1 gcggtttggc acgccaggca aacaccttgc tatgtatcct cttcatgaac cacagccctg
       61 agaggtatta ggagttttga tatgaacaat ttcgtccaga tcgccttaaa aaagccttat
      121 acatttgtgg tcttggccat cctcatcttg gtgttcgggg cactgacagt agttgaggca
      181 cccaccgacg ttttcccaaa tatccagatg cctgttagct ccgtggtctg gctctacgat
      241 ggcttaatgc cgcaagagac cgaaggccga gtcacacgtg tcttcgaaca aatgttgact
      301 cagacggtcg aaggcatcaa gaccatcgtg agcacttctt acttcggtca ctccatcatc
      361 aatatttttc tccaagacgg ggttgatcta gccggcagcg aagccgatat cgtgggaata
      421 tcgcaaacct ccgtcaaggc cctaccccca gatgttgccg cgccaatggt catgcggctg
      481 tttccctccc aagtgccggt agccgtgtta caagtcgaat ccgatgtaca gacaccggcg
      541 gagctctaca atctctgcgt catgcgaatt cgacccatgc tcgtcacgat acccggcgcg
      601 atcctgccgc acccctatgg cggtcaggac atgcaggtca tgatcaatgt cgaccaacag
      661 aaattgctgg cccgtcatct caccacggag gacgtgcatc aagcgatcga aagacaaaat
      721 ctcgtgctgc ccggcggaga catgaagatc aaggagaccg actggcttgt cctgacgaac
      781 gcatctccgt tgaagattga agagtttgac gatatcccga tcaagcgaga gggcaacgcc
      841 ttcattcatc tgcgcgacgt cgccacggtg cgccttgccg gcagagtaca aacgaactcc
      901 gtgctggtcg acggcaaaca ggccgtcatc gtcgttgtca tgaagagcag tgaagcatcg
      961 accatagaca tcgtggatgg aatcaaaaaa atgctcccac gcatccagga ggtcgtgccc
     1021 cctgatgtaa aagtgagtct catcagcgac tcctcgatct tcgtaaagga tgctatcagc
     1081 gatgtggtgc atgaaatggt catcgccgcc gcgctggtcg gcttcatcgt gctgctgatg
     1141 cttggctcct ggcggccaac tgttatcgtt ctcacctcaa tcccactctc catcctcagc
     1201 tccatcatat gcctgcatct actcgaggaa tcaatcaata ttatgaccct aggtggattg
     1261 gcattggccg tcggcattct cgtcgataac gccgcggtca tgatcgagaa t
//
LOCUS       DASTWE010000391         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_334195, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000391 DASTWE010000000
VERSION     DASTWE010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334195"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFT30_04160"
     CDS             <1..350
                     /locus_tag="VFT30_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEU5091852.1"
                     /translation="RYLYFARRADIEGYPDLGGLFRDTSEAETGHAFGHLDFLKEVGD
                     PATGVPMGNTDANLKSAIEGETYEYTQMYPGMAKTARDEGFPELAEWFETLAKAERSH
                     ANRFQKGLDTLKA"
     gene            451..1728
                     /locus_tag="VFT30_04165"
     CDS             451..1728
                     /locus_tag="VFT30_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU5091853.1"
                     /translation="MKGLSLITPIDSKQLEKETLRIYEVCDGCRRCFNLCPSFNTLLD
                     RIDVHEGNVAKLTPADHHQVVDECYYCKLCFNHCPYTPPHHYEIDFPHLMVAWKKRLA
                     AERGVRWRDRLLIMTDVIGKFCSATASITNSLLENRLVRSLLDRVMGIHQDRRVLHFS
                     SETFPRWFNRRTKTATADPPVRKVALFASCLVNFQTADVGKATVQVLEKNGVHVVVPE
                     QRCCGMPSFDIGDIQAIQQAARSNIASLYPLVVNGYDVVVPTASCSLMLKREYPELHR
                     DEKTKRVAGRTFDVCEYLMAMKKTGHLATDFSVKPGRIAYQIPCHLRDQNIGFKSKEL
                     MECAGAQVEVIEHCSGHDGAWSAKTEFFPLSMKIAGKAVRAIEQTPADFVASDCPLAG
                     LQLEQAGASAHVTEKTVLHPIQIVRDAYGLDKG"
BASE COUNT          398 a          457 c          524 g          401 t
ORIGIN      
        1 gccggtatct gtatttcgcg cgaagggccg atattgaagg ctatccggac ctcggcgggc
       61 ttttccgaga cacctctgag gctgaaacgg gccacgcttt cggtcacttg gattttttga
      121 aagaggtggg agatccggca acaggcgtgc cgatgggcaa caccgatgcc aatctcaaat
      181 ccgccatcga gggtgaaacc tacgagtaca ctcagatgta tcccgggatg gccaagaccg
      241 ctcgagacga agggtttcct gagttagccg agtggtttga aaccttggcg aaagctgaac
      301 gatcccatgc caatcggttt cagaaggggc tggatactct gaaggcttaa cagcctgcgg
      361 gtcagttgac cttcagcggt cagttttcgg cgacgtggta cgagttcgtc ggcgaaggct
      421 ggccgctgat cgttttcgga gaagcgaatc atgaagggtc tcagcctcat cacaccgatc
      481 gattccaaac agttggagaa ggaaacgctg cggatctacg aggtctgtga cggttgccgg
      541 cgctgcttca atctttgtcc gtcgttcaac accctcttgg atcgtattga tgtgcacgaa
      601 ggtaatgtgg ccaagctgac cccggccgac catcatcaag tcgtcgatga atgctactac
      661 tgcaagctct gtttcaatca ttgtccgtat actccgccgc atcactacga aatcgacttc
      721 ccgcatttga tggtcgcctg gaagaagcgt ctcgccgcgg aacgtggtgt ccgatggcga
      781 gaccgcctat tgatcatgac agatgttatc ggaaaattct gcagtgcaac cgcctccatc
      841 accaacagtt tgttggagaa ccgacttgtg cgcagccttc tggaccgagt catggggatt
      901 catcaagacc gccgagtcct gcatttttcc agtgagacat ttccccgatg gttcaaccgt
      961 cgaaccaaga cagccacagc cgatccaccg gtccgcaagg tggccctgtt tgccagttgc
     1021 ctcgtgaatt ttcagacggc tgacgtcggc aaggcgacgg tgcaggtatt ggagaagaac
     1081 ggagttcatg tggtggtgcc ggagcaacgt tgttgcggca tgccgagttt tgatattggg
     1141 gatatccagg ctattcaaca ggcggctcga agcaatattg cttcattgta ccccttggtc
     1201 gtcaacggat atgatgtggt ggtccccacc gccagctgta gcttgatgtt gaaacgcgaa
     1261 tatcctgagc tccaccgtga cgagaagacc aagcgggttg cggggcggac tttcgatgtg
     1321 tgtgaatatc tgatggcgat gaaaaaaacc ggccatctgg cgaccgattt ctcggtgaag
     1381 cctggacgaa tcgcctatca gatcccctgt cacctgaggg accagaatat cgggttcaag
     1441 tccaaggaac tgatggaatg cgccggtgcc caggtcgagg tgatcgaaca ttgttcgggg
     1501 catgacggcg cgtggtccgc caaaacggag tttttcccgt tgtccatgaa aatcgccggc
     1561 aaggccgtgc gcgcgatcga gcaaacaccg gctgatttcg tcgcatcgga ttgtccgttg
     1621 gcggggttac aattggagca agccggcgcg tcggcccatg tgacggagaa gaccgtgcta
     1681 caccccattc agatcgtccg tgacgcctac gggctggata agggctaaac ggaggagttt
     1741 ccatgaatgg cggagatgtc gacaaatgat caatgtgtgg
//
LOCUS       DASTWE010000392         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1109838, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000392 DASTWE010000000
VERSION     DASTWE010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109838"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /gene="gspN"
                     /locus_tag="VFT30_04170"
     CDS             complement(<1..320)
                     /gene="gspN"
                     /locus_tag="VFT30_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HEU5091854.1"
                     /translation="MTIKWPEAWREILAWTVGGISILALCLIATFPYSMLQARVVAEL
                     TRATGMEVRVADWTMGLPLGLEWRNVTLSQPNWTPIQLTMLQAKVGVMKALGGTLGLD
                     VVATL"
     gene            complement(317..940)
                     /locus_tag="VFT30_04175"
     CDS             complement(317..940)
                     /locus_tag="VFT30_04175"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEU5091855.1"
                     /translation="MSSPAVSRFKSEAGFTLVEVLVAVALLGMIGAMVFGSLVMTTNA
                     VEAGREQAAKEQAIRRILRLMAEEISLSKRNTMYPWVGTNGTQDGQPADILAFLAMSQ
                     ELSTSAVKESETVRVVYTRERDRLIRFVRRNLYTLTDTNESLDQMELADRVQAFNVRY
                     YDDQNRMWIDEWPAVPKIPKALLIEVTFQYPDAAPWTVREWVAIGTS"
     gene            complement(912..1349)
                     /locus_tag="VFT30_04180"
     CDS             complement(912..1349)
                     /locus_tag="VFT30_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5091856.1"
                     /translation="MARDRNRTKSNEGGFTLLEVLLAIALLAIALPILLGLRNFDLEL
                     QDRASELTTATLLAQEKLLETELSGQYAIGESTGDFPNIPLGAQMTMQAVPRAVGYKW
                     KRTIAPTPLELIREIRIKVSWLRGQLEEAVEVSTYVFAGRLTF"
     gene            complement(1356..>1553)
                     /locus_tag="VFT30_04185"
     CDS             complement(1356..>1553)
                     /locus_tag="VFT30_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091857.1"
                     /translation="VTMYLMDEKNNLLGLAVDSLTGWIRVSDERLDPPPNRIIPDRVR
                     LLLKPNKQAGALPRGLFPIQQ"
BASE COUNT          315 a          454 c          416 g          368 t
ORIGIN      
        1 agcgttgcga cgacatcgag tccaagcgtg ccgcccaagg ctttcatcac tccaactttg
       61 gcttgcagca tcgttaattg aatcggggtc cagttcggtt gcgacaaggt gacgttccgc
      121 cattcgagac ctaaagggag ccccatggtc caatccgcga ctcggacctc catgccggtg
      181 gcgcgtgtga gttccgcaac gactcgcgcc tggagcatgc tgtacgggaa cgtcgctatt
      241 aggcacaacg ccaagatgga gattccccct accgtccaag ccaggatctc tcgccatgcc
      301 tcaggccatt tgatagtcat gatgtaccga tcgccaccca ttcccgcacc gtccatggag
      361 cggcatcggg gtattgaaac gtcacttcaa tcagcaaggc cttggggatt ttggggactg
      421 ccggccattc gtcgatccac atccggttct gatcgtcgta atatcggaca ttgaaggcct
      481 ggacacgatc ggctagttcc atctgatcca acgactcatt ggtatcggtc agcgtataga
      541 gatttctgcg gacgaaccga atcaaccgat cacgttcacg cgtgtacacc acgcgtacgg
      601 tttcactttc cttgacggcc gatgtgctca attcttgact catcgcgaga aaggcgagga
      661 tgtcggccgg ttgtccgtct tgtgttccat tcgttcccac ccagggatac atggtattgc
      721 gtttgctgag ggagatttcc tcggccatga gacgcaaaat tcttctgatc gcttgttcct
      781 tcgccgcttg ctctcgtccg gcctccaccg cgttcgtcgt catgacgagc gacccaaaga
      841 ccatggcgcc gatcatgccc agcaaggcca cggcgaccaa gacttcgacg agcgtaaagc
      901 ccgcttcgga tttaaaacgt gagacggccg gcgaagacat acgtgctgac ctctaccgct
      961 tcttccagtt ggccccgtag ccacgagacc ttgatccgaa tctcccgaat tagctccagc
     1021 ggggtgggcg cgatcgtacg tttccacttg tatccgactg ctctcgggac tgcctgcatg
     1081 gtcatttgtg ctccaagagg gatgttcgga aaatctccgg tgctttctcc gatggcatac
     1141 tgtcctgaga gctccgtttc taggagtttc tcttgggcca acagcgtggc cgtggtcagt
     1201 tcggacgctc tatcttggag ttccaggtcg aaatttctaa gaccaaggag aatcggcaga
     1261 gcgatggcca gcaaggcgat ggccagcagt acttcgagca gcgtgaatcc gccttcgttg
     1321 gacttggttc ggtttctatc tcgggccatc gcgatctact gttgaatcgg aaacaatcct
     1381 ctcggaagcg ctcctgcttg cttattcggc ttcaacagaa gccggactcg gtcgggaatg
     1441 atccggttcg gcggagggtc gagccgttcg tcgctgaccc gaatccatcc cgtcaacgaa
     1501 tcgactgcca gccccaagag gttattcttc tcatccatga gatacatcgt gac
//
LOCUS       DASTWE010000393         1211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_43380, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000393 DASTWE010000000
VERSION     DASTWE010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43380"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1094
                     /locus_tag="VFT30_04190"
     CDS             165..1094
                     /locus_tag="VFT30_04190"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU5091858.1"
                     /translation="MIDLSTTIAGVTFPSCFMNASGALCVTRDELEALGKSGTGAIVT
                     KSMTIESRQGNPTPRYYGFPGGSINSMGLPNLGYRAYAELIPHLKRFGKPVIASVAGL
                     AEEDFPTIAEAINAAQPDLIEVNLSCPNIPGKPQIGYDPETSERVLKKVRRLIAVPMG
                     VKLPPYFDPAHHETMGKVVGRCGVDFLNMINSVGNGLVVDPEEETVVIKPKGGFGGLG
                     GRLIKPVALANVRAFHKFFEGKIPIIGTGGIVEGIDVFEHFLCGASAVQIGTVLVEEG
                     LNVFGRLEAELTAVLARKGYRSILECRGRLKEL"
BASE COUNT          271 a          293 c          373 g          274 t
ORIGIN      
        1 gcgggtatta tgtcgcttat ccgtgggctc gcgatatccg gagggcaaac ggtactgctg
       61 gttacacaca gcttgcaggc cgcgcaagcc gccgactatg tttggactat gcgagatgga
      121 cagttggttt cccgcactga acgtatgacc gaactggtaa ctccgtgatc gacctctcca
      181 cgacaatcgc cggcgtgacc tttcccagct gtttcatgaa tgcgtcgggg gcgctctgtg
      241 ttacacggga cgaacttgag gccttgggga aatccggtac gggggcgatc gtcacgaagt
      301 cgatgacgat tgagtcacgc caaggaaacc caacgcctcg gtattacgga tttcccggcg
      361 gatcgatcaa ttcgatggga cttcccaacc tgggctatag ggcctacgcc gagttgatcc
      421 ctcacctcaa gcggttcgga aagcccgtca tagccagcgt cgccggactc gcagaagagg
      481 actttccgac gatcgcggaa gcgatcaatg cagcccagcc ggatctcatt gaggtcaatc
      541 tatcttgtcc aaatattcca ggtaagcccc aaattggcta cgatccggaa acttctgagc
      601 gggtccttaa gaaggttcga cgtctcatcg cggttccgat gggagtaaag ctgccgccgt
      661 actttgatcc ggcgcatcat gagaccatgg ggaaggtagt tgggcgttgt ggagtcgact
      721 ttctcaatat gatcaattca gtgggcaatg gcctggttgt cgatccggaa gaggaaaccg
      781 tcgtcattaa accgaaagga ggatttggtg ggttgggcgg gcgactcatc aagcccgtgg
      841 cattggcgaa cgtccgcgcc ttccacaaat tttttgaggg gaagattccg atcatcggaa
      901 ccggaggaat cgtagagggg atcgatgttt tcgaacattt cctttgcgga gcttcggcgg
      961 tgcaaatcgg gacggtgttg gtggaagagg ggctgaatgt gttcggccga ctggaagcgg
     1021 aattgactgc tgtcttggca agaaaaggat accggtcgat tctggaatgc cggggacggc
     1081 tcaaggaatt atgaacctat ttattaccga agttgcgagg cagcaggtgt tgttgtaggg
     1141 cctgacgcag gagttgggcg acgttccgaa cattgagtcg gcgcatcaag ttgaaacgat
     1201 ggacttcgac c
//
LOCUS       DASTWE010000394         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1238970, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000394 DASTWE010000000
VERSION     DASTWE010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238970"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="VFT30_04195"
     CDS             complement(<1..1883)
                     /locus_tag="VFT30_04195"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU5091859.1"
                     /translation="MMELDPKIPSGPLETKWDRHRFSEKLVSPNNKRNITVIVVGTGL
                     AGASAASTLGQLGYQVECFCFQDSPRRAHSIAAQGGVNAAKNYQDDGDSVGRLFYDTI
                     KGGDFRSREANVYRLAQVSTQIIDQCVALGIPFAREYGGQLANRSFGGVQVSRTFYCR
                     GQTGQQLLLGAYSALCLQIERGQITMRPRTEMLDLVVIDGQARGVVARDLVTGRVSTH
                     TAHAVVLATGGYSNVYYLSTNASGSNVTATYRAWKQGAAFANPCFTQIHPTAIPPGDA
                     HQAKLTLMSESLRNDGRLWVPKMARDYRSPEDIPAAERDYFLERRYPRFGNLAPRDLA
                     SRAVKVVCDEGRGVGPGGHGVYLDFADVIAQQGLDVVRERYGNLFDMYQRITGEDAFH
                     TPMRIYPAPHYTMGGLWVDYNLMSTIPGLFVIGEANFADHGANRLGASSLMQGLADGY
                     FILPYTIGHYLATAKFTPVGEEHGESRAALQRVQNRIGRLLNSKGHRTAASFHREVGK
                     LLWNHCGMSRNEEGLKSALTSIPALREEFWRDVMVPGSGQAFNQELEYAGRVADYLEF
                     AELLCHDALHRKESCGAHFREEYQTDEGEAQRDDSRFAHVAAWEYREGASPILHKEPL
                     AFE"
     gene            complement(1880..2569)
                     /locus_tag="VFT30_04200"
     CDS             complement(1880..2569)
                     /locus_tag="VFT30_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEU5091860.1"
                     /translation="MARPSNHSNHFQSTVGSKVVMALSGLGLVIYVIFHMLGNLQVFE
                     GSHALNGYAAFLRDMPILLWAARAGLLSIVVLHIMLAIQLTLRNRHARPVAYAVREYR
                     QASFASRTMATSGLVLLLFIIFHLLHLTAGVIDPSSADRLDAEGHRDVYGKIVHAFQN
                     PFIVALYLVGQLGLGLHLNHAVTSSLQTLGFEHAAFNRLFKAAGPTVALLVVLGNAAI
                     ILAIFLGIVRG"
BASE COUNT          648 a          781 c          746 g          573 t
ORIGIN      
        1 aattcaaaag caagaggctc cttgtgtaag atgggcgatg caccttctcg gtattcccat
       61 gcggccacat gcgcgaagcg gctatcatca cgttgcgcct cgccctcgtc cgtctgatac
      121 tcttctcgga agtgcgctcc gcatgactct ttccgatgca acgcatcatg acaaaggagt
      181 tctgcgaatt cgagatagtc cgccactcgc cccgcatact ccaattcttg gttgaaggct
      241 tgtcctgacc ccggaaccat cacatcccgc caaaattctt cccgcagtgc cggaatcgac
      301 gtcagtgccg atttgagccc ttcttcattg cgggacatgc cacagtgatt ccacaacagc
      361 ttaccgactt cccggtgaaa ggaggcagcc gtgcgatgcc cctttgaatt cagaaggcga
      421 ccgatccgat tctgcacgcg ctgaagcgct gctcgcgatt ccccgtgctc ttcaccgaca
      481 ggagtgaatt tcgccgtcgc caaataatga ccgatcgtat acggcaagat aaaatagcca
      541 tcggcaagtc cttgcatgag agagctggct ccgagccgat tggcaccgtg atccgcgaag
      601 ttcgcttcgc cgatcacaaa gagtcccggg atggtactca tgagattgta atccacccag
      661 agccctccca tcgtatagtg cggcgccggg taaattcgca tgggtgtatg aaaggcatct
      721 tcccctgtga ttcgctgata catgtcgaag agattgccgt aacgctcgcg gaccacatca
      781 agcccctgct gggcgatgac gtcggcgaaa tccaggtaca cgccgtgacc gcccggccca
      841 actccacgac cttcgtcgca cacgaccttc acggcacggg atgcgagatc tcggggtgcg
      901 agattgccaa aacgtgggta tcggcgttcc agaaagtagt cgcgttccgc cgcgggaata
      961 tcctccggag accggtaatc tctcgccatc ttcggaaccc acaatcgtcc gtcgttgcga
     1021 agcgattcag acatcagggt caatttggcc tgatgggcat cccccggtgg aatcgctgtg
     1081 gggtggatct gcgtaaaaca aggattcgcg aatgcggcgc cctgcttcca cgcgcgatag
     1141 gtggccgtca cgttgctgcc gctcgcattg gtcgacagat aatacacatt gctgtagccg
     1201 ccggtcgcaa gaaccacggc atgggccgta tgggtgctca cccgtcccgt gaccagatcg
     1261 cgcgccacga ctccccgagc ctgcccgtcg atcacgacaa gatcgagcat ctcggtgcgt
     1321 ggacgcatcg tgatctgtcc tcgctcaatc tgcaaacaga gcgcagaata agcacctaag
     1381 agaagctgct gtcctgtctg tcctcgacaa tagaacgtcc gtgagacttg gacgcccccg
     1441 aacgaacgat ttgccaactg ccctccgtac tccctggcaa acggaatgcc gagcgccaca
     1501 cattgatcga tgatttgtgt actcacctgg gccaatcgat acacattggc ctcgcgagag
     1561 cggaagtctc cacctttgat tgtgtcatag aatagccggc ccacactgtc tccgtcgtct
     1621 tggtaattct tagcggcatt gactcctccc tgtgccgcaa tgctgtgggc gcggcgcgga
     1681 ctatcctgaa aacaaaagca ttccacctga tagcccaact gtcccaatgt tgaagcggca
     1741 ctggctcccg caaggccggt accgacaaca atgaccgtga tgttccgctt gttattcgga
     1801 ctcaccagtt tttcgctgaa ccggtgccgg tcccatttgg tttccagcgg gccggagggg
     1861 atttttggat caagctccat catccgcgta caatccccaa aaagatggcg aggatgatgg
     1921 cggcattgcc aagaacgacc aacagcgcga ccgtaggacc agcggccttg aacagtcggt
     1981 tgaatgcagc atgttcaaat cccaatgtct gcaaactact ggtgacggca tggttcagat
     2041 gcaagcccaa acccagttga cccacgaggt agagcgccac gatgaatgga ttctgaaagg
     2101 catgaacgat ttttccatag acatcacgat gtccttcagc gtcgagacgg tcggcagaag
     2161 aaggatcgat gacgccggcc gttagatgca acaagtggaa gatgatgaag agcaagagca
     2221 caaggccgga ggtggccatc gttcgtgaag cgaatgatgc ctggcggtac tcacggaccg
     2281 cataggcaac ggggcgggca tgacggtttc ggagggtcaa ctggatcgcc agcatgatat
     2341 ggagaaccac aatgctcaat agcccagcgc gagctgccca gaggaggatc ggcatgtcgc
     2401 ggagaaacgc cgcatagcca ttgagcgcat gggatccttc gaatacttga agatttccca
     2461 acatgtgaaa aataacatag atcaccagcc ccaacccgct cagggccatg acgactttac
     2521 tgccgactgt ggattggaaa tggttagaat ggttagatgg acgtgccatc gggaagcatt
     2581 atacactgct tgaatttaag aacaggtaca ggagcctgcg tcgcgtggtg tatgggggga
     2641 ttgattacct atctagcagt caatgaattt gagcgtggag agacctgcct aacttgaagg
     2701 tcgagttgct cggaaggttt caaacctgga accaatacat cgagcgcc
//
LOCUS       DASTWE010000395         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_75642, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000395 DASTWE010000000
VERSION     DASTWE010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75642"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFT30_04205"
     CDS             complement(<1..219)
                     /locus_tag="VFT30_04205"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEU5091861.1"
                     /translation="MGSDAAKLLEAALKLPPEARAAMAASLLESLDTVIDADAEAEWD
                     REIARRLKDLDAAPARVISWSDARRKILG"
     gene            complement(790..>1005)
                     /locus_tag="VFT30_04210"
     CDS             complement(790..>1005)
                     /locus_tag="VFT30_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEU5091862.1"
                     /translation="LTAQESDRLARLARILTYATEVFGTAEKASMWVSRSNRILQGTA
                     PIELLDTDLGTQVVETMLGRIEHGVIS"
BASE COUNT          253 a          258 c          275 g          219 t
ORIGIN      
        1 accgagaatc tttcggcggg catcactcca ggagataaca cgggcaggag ctgcatcgag
       61 atccttaagc cgacgagcaa tttcacgatc ccattctgct tctgcgtcgg catcaatgac
      121 tgtatcaagg ctctctaata acgaagcggc cattgcagcc cgagcctcag gcggaagctt
      181 caatgcagct tccagtaatt ttgcagcgtc agaacccatg agtgaatagt agcagaaatc
      241 gggaaagtag gacagctgga taggtcggca ctagacaagc ggctttgact gattcggaag
      301 cgcgggcgcg aagagtttta tcagccaaag gcgaatctta cttaagaagc cgcaggtcgg
      361 aagaaaagcg ggttccagtg tcccatgtaa ttctagaaaa ctccggatgg gcaggattta
      421 gcacataatt ccactcatgc gggatcacca ctgagggaac tttgagcacc ggactttgcg
      481 cccgtacgac ccatgccgaa ccgagctcct gcaagctcac agggatggtt gtatctctcc
      541 aatcgtcagg gaggtccgat atctggattg tttccattgc aagactatcc ggtatggtca
      601 ctcgaaagtt gagcaacgaa tctggaagga tgtcggaatc aacgtgaacg agatattcga
      661 gaacggcaag agataacgta ccggacgtgt agatgacggg tgttccagac ctattccacc
      721 gtccaccgta gcgtcgagcc ccttctccgt ccgaagccgc atgccccttc agagtcaggc
      781 gccagacaac taggatataa caccatgttc aatgcgtccc aacatcgtct cgacgacttg
      841 cgtgccaaga tcggtatcga ggagctcgat gggggcggtt ccttgcagga ttcgattcga
      901 tcggcttacc cacattgaag ccttctctgc cgtaccgaag acttcggtgg cataggttag
      961 gattcttgca aggcgtgcca agcggtcgga ttcttgtgcg gtgag
//
LOCUS       DASTWE010000396         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1013135, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000396 DASTWE010000000
VERSION     DASTWE010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013135"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFT30_04215"
     CDS             <1..241
                     /locus_tag="VFT30_04215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5091863.1"
                     /translation="ITSIGYDPKLGNIVRVDHGFGVETLYGHLAKALVKEGQRVERGD
                     VIALVGSSGLSTGPHLHYMVKLNGQALDPTKYILE"
     gene            400..2082
                     /locus_tag="VFT30_04220"
     CDS             400..2082
                     /locus_tag="VFT30_04220"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU5091864.1"
                     /translation="MTDLEIAQSVTPRPIVEIGSQLGICAEELTPYGRDKAKVSLQVL
                     DRLMTAPKGRYVLVTAINPTPLGEGKTTTSIGLAMGLSRLGHRAALTLRQPSLGPVFG
                     VKGGGSGGGHAQVAPMEDINLHLTGDAHAVAASHNLLSAFLDNHLFHGNTLSIDPQRI
                     TWPRTLSISDRALREILLGEETDRRPGQFVITEASEVMAALALANDQCDLRERLGRIL
                     VGLTKAGTPVTAEELHCVGSMAVLLKDALMPNLVQTLEGTPAFVHTGPFGNIAHGNCS
                     IISDAIALRCADFVVTEAGFGSDLGAEKFFNIKCRASGFAPTVAVIVATLRALKLHGG
                     GGVAKAGVALPVSLTGPNREALSKGIANLEQHVANVLAHGVPVVVAVNAFKDDPRDEL
                     EWVREQSLKMGAADAAVSTHWADGGKGAEQLAAAVVKTSERPAHFRHLYDLSWPIRKK
                     IETIATRMYGAAGVTFEPKAERQIETAEALGYGGLPVCMAKTPLSLSHDPGLKGRPTG
                     FMVPIRELRILAGAGFVTAVCSGIQLMPGLPKKPAGERINLDSNSGKIVGLS"
     gene            complement(2086..>2357)
                     /locus_tag="VFT30_04225"
     CDS             complement(2086..>2357)
                     /locus_tag="VFT30_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEU5091865.1"
                     /translation="AEGAEEAQKIRSEAEKDREIILAQAYKESEELRGGGDAKAFRIY
                     ADAYRQDLKFFEFTRSMEAYKSAFKDGSTLVMSPDSEFFRYLRQR"
BASE COUNT          526 a          647 c          662 g          522 t
ORIGIN      
        1 gattacgtcc ataggatacg atcctaagct tgggaacata gtccgcgtag atcatggatt
       61 cggagttgaa actctttatg ggcaccttgc gaaggccctg gtaaaggaag gtcagagggt
      121 cgagcgtggc gatgtcattg cactcgtcgg gagctcgggc ctgtccaccg ggcctcatct
      181 acactacatg gtgaaattga acggccaggc acttgaccca accaagtaca tcctagagta
      241 ggaacgtagc gagttcgacc gacctgtttc tgctgcaccc cttggtcagt ccaattattc
      301 ccagccatat tccatattcg ttgcttgctt ccaatcattg gccgactgcc tatgctatac
      361 tcggaccctg caatctagtc aaaaccagat gagagcctct tgaccgacct agaaatcgcc
      421 caatctgtta cccctcgtcc cattgtcgaa atcggttccc agcttggaat ttgtgccgaa
      481 gagctcacgc cctatggacg ggacaaggcg aaagtctctc tacaagtact ggatcgtctg
      541 atgactgccc cgaaggggcg gtatgttctt gtcacggcaa ttaaccccac gcccttggga
      601 gagggaaaga ccacgacgtc gataggtctt gccatgggac tctcgcggct aggacatcga
      661 gccgcgctta ccctcaggca gccatcgctc ggtcctgtgt ttggggtgaa ggggggaggg
      721 agtggtgggg ggcatgccca agtcgctccg atggaagata tcaaccttca cctgaccggg
      781 gatgcccacg ccgtggccgc cagccacaat ctgctctcag cgttcctcga taatcatctg
      841 ttccatggca atactctttc gatcgatcct caacggatta cgtggcctag aaccttgagt
      901 atcagtgatc gcgcgctccg cgagatcctg ttgggagaag aaacggacag acgcccagga
      961 caattcgtca tcaccgaggc ttcagaagtg atggcagcgc tggctcttgc gaacgatcag
     1021 tgtgatctcc gagagcggct cggtcgaata ctcgtcggac tgacaaaggc cggtacccca
     1081 gttaccgctg aagagcttca ttgcgtggga tcgatggctg ttctcctaaa ggatgccctc
     1141 atgccgaatc tcgttcaaac ccttgaaggc actccggcat tcgtccatac gggacccttc
     1201 ggcaacatcg cgcatggaaa ctgctcaatc atttccgacg cgatcgcgct gagatgcgcc
     1261 gatttcgtcg tcacagaggc aggcttcggc agcgatcttg gggcggaaaa attttttaac
     1321 atcaagtgtc gcgcatcggg atttgcaccg acagtagcgg tgatcgtggc gacattacgg
     1381 gcgctgaaac tccatggcgg tggaggcgtt gccaaggcag gtgtcgcctt gcctgtgagc
     1441 ctaaccggtc cgaatcggga agcgctctcg aagggaatcg ccaacctgga gcagcatgtc
     1501 gccaacgtcc tagcacacgg ggtacctgtc gttgtcgccg tcaatgcctt caaggatgat
     1561 cctcgggatg aattggaatg ggttcgagaa cagtcactca agatgggggc ggcagatgcg
     1621 gcagtctcaa ctcactgggc cgacggagga aagggagctg aacagctggc cgccgccgtc
     1681 gtcaagacat ctgaaagacc ggctcacttc agacatcttt acgatctgtc ttggccgatt
     1741 aggaaaaaaa tcgaaaccat tgcgacccgc atgtacggag cggccggagt gacgtttgaa
     1801 ccgaaggccg agcgtcaaat tgaaacagca gaggccttgg ggtacggcgg cctacccgtc
     1861 tgcatggcaa aaactcctct gtcgctgtca cacgatcctg gactgaaggg gagaccaacc
     1921 gggtttatgg tgccgatccg agaattgcgg attttagccg gagcaggatt tgtgactgcc
     1981 gtctgctcag gaattcagct catgccggga ctgcccaaga aaccagccgg tgagcggatc
     2041 aacctcgact caaatagcgg aaagattgtg ggactttcct gaaattcagc gttgcctgag
     2101 atagcggaag aattctgagt ccgggctcat caccaaagtc gagccgtctt tgaaggcgct
     2161 cttgtaggct tccatcgagc gggtaaactc gaaaaacttc aggtcttgcc ggtaagcatc
     2221 ggcatagatt cgaaacgctt tggcgtcgcc gccgccgcga agctcttccg actctttata
     2281 ggcttgggct aggatgattt cccggtcctt ctcggcttct gatctgattt tttgagcttc
     2341 ttccgctccc tccgccc
//
LOCUS       DASTWE010000397         5224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_269458, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000397 DASTWE010000000
VERSION     DASTWE010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269458"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFT30_04230"
     CDS             complement(<1..854)
                     /locus_tag="VFT30_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5091866.1"
                     /translation="MDTTQLVGTRVTVVGLARSGVAAVRLLQEAGALVTVADRKDRGE
                     LLGVLGSLDQAATRLVLGSDYESALDQAELVVISPGVPYRLEALERVRRRGVKVISEL
                     DLASRFLSAPILALTGTNGKSTTVTLIGKMLQESGKRIFVGGNLGTAISEAAVQSLEA
                     MKMGRPCPYDALVVEVSSFQLETIEQFHPWIAAILNVTVDHQDRYESIDEYIATKNRI
                     FENQTQSDYALFNLDDSRVAPLRQSARASVLGFTKTQTLPSDLAGGTYLDRDRIMTTI
                     GGRTQEICA"
     gene            complement(869..1945)
                     /gene="mraY"
                     /locus_tag="VFT30_04235"
     CDS             complement(869..1945)
                     /gene="mraY"
                     /locus_tag="VFT30_04235"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5091867.1"
                     /translation="MLYIWLYPLHTEFSFLNVFRYQSFRIIYAAVTAFLIAFVLAPWV
                     IRKLQEIKLGQQIRDDGPKRHLAKSGTPTMGGILIIFAVTLSTLLWADISRPHIWLVL
                     VATLGFCAVGFVDDYLKFIKAQSKGLSASQKFTAQIVIALIVALILYSLPGYNTKLSV
                     PFFKNFMPDLGWFYVVFAVIVIVGSSNAVNLTDGLDGLAIGPVMIAALAYTVVAYVTG
                     HRLMSEYLLIQHIDGAGELAVFTAAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVISKHELLLLMVGGVFVIEAVSVIFQVASYKSRGKRIFLMAPIHHHFEMKGWEEP
                     KVVVRLWIIAILLALLSLSTLKLR"
     gene            complement(1960..3408)
                     /gene="murF"
                     /locus_tag="VFT30_04240"
     CDS             complement(1960..3408)
                     /gene="murF"
                     /locus_tag="VFT30_04240"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5091868.1"
                     /translation="MTLFTVEELREVVGARVLTGEGIGRAKHSIRRIGLDTRSLRPGD
                     LFLALRGDRFDGHDFVATALSRGAVGAIVLDSYDVSELALKRGSKRTQPFILGVSDPL
                     YAYQQLAAHHRSRFQIPVIAVTGSNGKTTTKEMVASVMAHNWKILKTEGNLNNRVGVP
                     QTLLRLNDRHKGAVIEMGVDNLGQTARLCEIARPTIGIITNIGPDHLEFFGTIEASAQ
                     AKAELLDFLPRDGTAILNADDPYYDYLAARARCRVVSFGLSSKADVRAMDVKSDGRNG
                     TIFRLVLPGTVRQTIVHIRVQGEHNVTNALAAGAVGSVLGLSGGVIAQGLSRFRPAAM
                     RSQVLMNQGVKLIIDCYNANPASMKAAVQLLAQTGAKRKKIAVLGDMLELGPNAAQMH
                     EEVGKFLARQGIDQLVACGVLGRSLAKGAKQAGLEPSRILEVPDAHTAAAAVKTIVKA
                     GDAVLIKASRGMKLELVADALQGAKRTTKKAS"
     gene            complement(3449..4951)
                     /locus_tag="VFT30_04245"
     CDS             complement(3449..4951)
                     /locus_tag="VFT30_04245"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5091869.1"
                     /translation="MTLATLLQALAGQVKILDRRGNFDVSVNAITDDSRAVSTDSLFV
                     AVKGERVDGHEFVLAAIKGGVAALVSQQPMREVSLPFARVDDSRKALGLLGSRFYGDP
                     SSHIRMIGVTGTNGKTTTTYVCKALLEAIGHPVGLIGTVVYQIGERTIPATHTTPGSL
                     ELQQLLAMMVAGQCTTAVMEVSSHALSQERTSGCEYDVAVFSNLTQDHLDFHKTMEEY
                     FQAKLRLFTGLKRGVKANKRAIINIDDPYGHRIVEQSPAPVWTYALKTKADLRAEAVR
                     LSLQGTTFTAATPVGSFPIESHLVGEHNVYNLLAAIGVALHEGATPAQIREAVARVSN
                     VPGRFERVMAGQPFTVAVDYAHTEDALVRLLTAAQALKTGRIITVFGCGGDRDRGKRP
                     KMGAAAVRYSDVVILTSDNPRTEDPLSILEQVEVGVIEALRQRSHVQYRKVPDRREAI
                     QEAVREAHSGDIVLIAGKGHEDYQIIGTKKVHFDDREVARNAIERLGTRV"
     gene            complement(4970..>5224)
                     /locus_tag="VFT30_04250"
     CDS             complement(4970..>5224)
                     /locus_tag="VFT30_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5091870.1"
                     /translation="GTAQKIDPRTGTYSASRFVASFTGYVPADNPQLAMIVVIDEPQG
                     DAWGGAVAAPVFSRVGEQVLSYLGVPSNDPVTLAMASRKS"
BASE COUNT         1180 a         1543 c         1341 g         1160 t
ORIGIN      
        1 gcacagattt cctgcgtccg gcctccaatg gtcgtcatga tacgatctcg atcgagatag
       61 gtgcctcccg ccagatcgga aggcagtgtt tgggtcttcg taaaccccag tacgctggcc
      121 ctcgcactct gccgtagagg agctaccctc gaatcatcca gattgaatag ggcatagtcc
      181 gactgagttt ggttctcgaa tattcggttt ttggtcgcaa tatattcgtc gatcgactca
      241 tagcgatcct gatggtccac cgtcacgttg agaatcgcgg caatccatgg atgaaactgc
      301 tcgatggttt ccagctgaaa gctggacact tccaccacca atgcgtcgta cgggcatgga
      361 cggcccatct tcatggcttc cagcgattgt acggcggcct cactgatcgc cgtgccaaga
      421 tttccaccga cgaatattcg ttttccgctc tcttgcagca tcttcccgat tagtgtgacc
      481 gtcgtgctct ttccattggt gccggtcaac gcaagaattg gagcggaaag aaaccgcgat
      541 gcgagatcga gttcactgat gaccttcaca cctcgacgac gaacacgttc aagagcttcc
      601 agccgataag ggactcccgg actaatcacg accagttcgg cctggtcgag agccgattca
      661 tagtcgctgc ccagcacgag gcgggttgcc gcctgatcca gagatccaag caccccgagc
      721 aactctcctc gatccttccg gtcggccacc gtcactaagg cgccggcctc ctgcaataag
      781 cggacagcgg ctacgccgct ccgtgccaac ccgacgactg tgacacgagt tcctaccaac
      841 tgcgtggtgt ccatctccac catcctcatc acctcaactt gagcgtgctc aggctcaata
      901 gcgccagcaa aatggcgatg atccacagac gcacgacgac tttcggctct tcccagcctt
      961 tcatctcaaa atggtgatga atcggagcca tgaggaagat ccgctttcct cgggatttgt
     1021 atgaggcgac ttggaaaatg accgagacgg cctcgatcac aaagacacca cccaccatca
     1081 gtaacaacaa ctcatgcttg ctgatgacgg cgaccgtccc aagggcggcc ccaagcggga
     1141 gcgaaccgac atctcccatg aagaccgagg ccggataggt gttgaaccaa aggaacccaa
     1201 gactcgaacc caggatggcc gccgtaaaga ccgccaactc tccggctccg tcgatgtgct
     1261 ggatcaggag atattccgac atcaatcggt ggccggtgac ataagcgacc acggtgtacg
     1321 ccaatgccgc aatcatcacc ggaccgatgg ctagcccatc gagaccatca gtgaggttga
     1381 cggcattgga acttcccacg atcactatca cggcaaaaac aacataaaac catcccaagt
     1441 cgggcatgaa attcttgaaa aacggcacgc tcagcttcgt gttgtagccg ggtaacgagt
     1501 acaaaatcag ggccacgatg agcgcaatga ctatttgcgc cgtaaacttt tgcgacgccg
     1561 aaagcccttt tgattgggcc ttgatgaatt tgagatagtc gtccacaaac ccaacggcgc
     1621 aaaaccccag cgtcgcgaca agaaccagcc agatatgggg acgtgagatg tctgcccaga
     1681 gcagcgtcga cagcgtgact gcgaagataa tgaggatgcc gcccatcgtg ggcgtcccac
     1741 ttttggccag atgtcgcttt ggcccatcgt cacgtatctg ttgccccagc ttgatttcct
     1801 gaagtttacg aatcacccac ggtgccagca cgaatgcgat caggaacgcc gtgacagcgg
     1861 cgtaaatgat gcgaaagctc tgataacgaa aaacattcag aaacgagaat tctgtatgga
     1921 gcggatagag ccagatatac agcatagtct gaacgatcgt tacgaagcct tcttcgtcgt
     1981 acgttttgct ccctgaagcg catcagcgac cagctctagc ttcatgccgc gggacgcttt
     2041 gatcaatacg gcatcacccg cttttacgat tgtttttacg gcggccgcgg cagtgtgtgc
     2101 atccggcact tcgagaatac gggatggctc cagtccggcc tgtttcgccc ctttggcgag
     2161 gcttcgccct aatacgccgc aagccacgag ctgatcgatg ccttgccgtg ctaaaaattt
     2221 gccgacttcc tcgtgcatct gggcggcatt cgggcccagt tccagcatgt ctccaaggac
     2281 ggcaattttc ttccgtttcg ccccagtttg cgccagcaat tgcaccgccg ctttcataga
     2341 tgcaggattg gcattgtaac agtcgatgat caacttcacg ccctgattca tcaatacttg
     2401 cgaccgcatg gcagcgggtc ggaaacggga cagtccttga gcgatcactc ctccggacaa
     2461 gcccaggacc gaaccgaccg ctccagcggc caaggcattg gtgacattgt gctcaccctg
     2521 gactcgaata tgaacaatgg tctggcgtac tgttcctggt aacacaagac gaaagatcgt
     2581 cccattccgc ccgtcggatt tcacgtccat cgcacggacg tcggcctttg atgaaaggcc
     2641 gaacgacacc acacgacatc gggctcgcgc ggcaagatag tcatagtacg gatcatctgc
     2701 attcaggatt gccgtcccat cacgaggaag aaaatcgagc aactccgcct ttgcttgagc
     2761 ggatgcctct atcgtcccga aaaactccag gtggtcgggg ccgatattcg taatgattcc
     2821 gatcgtgggt cgtgctattt cacacagcct ggcggtttgg ccgagattat cgactcccat
     2881 ctcaataacc gcacccttgt gtcgatcgtt gaggcgaagc agggtctgtg gaacacccac
     2941 acggttgttc agattgcctt cggtcttgag gatcttccag ttatgagcca tgacactggc
     3001 gaccatttcc ttcgtcgtcg tcttgccgtt gcttcccgtg acagcaatga ctggaatttg
     3061 aaatctgctt cggtggtgtg cggccagttg ctggtaggcg tagagtggat cgctgacgcc
     3121 gaggataaac ggctgggtcc gcttcgagcc acgcttcagg gcaagctccg acacatcgta
     3181 ggaatcgagc acgatcgctc caaccgcccc gcgcgacagc gctgtggcca caaagtcgtg
     3241 accgtcgaat cgatctcctc gcagtgcgag aaagagatcc ccgggtcgaa gagaccgagt
     3301 atccaagccg attcgccgga tagagtgctt ggccctaccg attccttcac cggtcaagac
     3361 tcttgcgccg accacttccc gcaattcttc gacggtaaac agagtcatct ggggcttccg
     3421 tgcaccgacc ggcctctgct cccatgagct agacacgggt tccgagtcgt tcgatggcgt
     3481 tgcgcgccac ctcgcgatcg tcgaaatgaa ccttcttcgt accgatgatc tggtagtctt
     3541 cgtgcccttt accggcaatt aagacgatgt ctccactatg ggcctctcgc accgcttctt
     3601 ggatagcttc tcgccgatca ggcacttttc ggtactgtac gtgagaccgt tgccgcagcg
     3661 cttcgatcac cccgacttct acttgttcca aaattgagag tggatcctcg gttctagggt
     3721 tatccgaggt caatatcacg acgtcgctgt agcgcacggc cgctgctccc atctttggcc
     3781 tctttcctcg gtcgcggtct ccaccgcagc caaagaccgt gatgatgcgt cctgtcttca
     3841 gcgcttgtgc ggccgtcaac aatcgaacga gcgcgtcttc ggtgtgagcg taatctacgg
     3901 ccaccgtaaa cggctggcct gccatcacac gttcgaaccg tcccggcacg ttactgactc
     3961 tcgccacagc ctcacgaatt tgcgctggtg tggctccttc gtgtagtgca accccgatgg
     4021 cagctagtaa attataaaca ttgtgctcgc caacgaggtg actctcgatg gggaagcttc
     4081 cgaccggggt cgcagcggta aaggttgttc cctgcagaga caatcgcaca gcttcagcac
     4141 gcagatccgc cttggtcttt aatgcatagg tccacactgg ggcggggctt tgctcgacaa
     4201 tacgatgtcc atacgggtca tcaatattga tgatcgctcg cttattcgcc ttcacccctc
     4261 tcttcaatcc tgtaaaaagt ctaagtttcg cttgaaagta ctcctccatg gtcttgtgaa
     4321 aatcgagatg atcctgggtc aggttcgaaa agaccgccac gtcatattcg catccgctcg
     4381 tgcgctcttg cgacagggcg tgagaagaca cttccataac tgcagttgtg cattgaccgg
     4441 cgaccatcat ggcaagcagc tgttgcagct caagagaacc aggtgtcgta tgcgttgctg
     4501 gtattgtgcg ctcaccgatc tgatagacga cggttccaat caatcctact ggatggccaa
     4561 tggcttccag cagcgctttg cagacatacg tcgtggtggt ttttccgttc gtcccagtca
     4621 cgcctatcat ccgaatatgc gacgagggat ctccataaaa acggctcccc agcagaccga
     4681 gcgcctttct ggagtcgtca acgcgggcga atggaagaga cacctccctc atcggctgct
     4741 gcgacaccaa tgcggccact ccccctttta tcgccgctag aacaaattca tgtccatcga
     4801 cccgctcacc ttttacggct acaaagagac tgtcggtcga gacggctcga gaatcatcgg
     4861 tgattgcgtt tacggacaca tcaaaatttc cacgacgatc aagaatcttc acctgtcctg
     4921 cgagggcctg aagcagggtt gccaaagtca tcgacatgtc caaatagaat tacgatttcc
     4981 gcgaagccat cgccagcgtg acaggatcat tcgatggcac gcctaagtag cttaacacct
     5041 gttccccgac acgactaaac acgggggcgg cgacagcccc accccacgca tcaccttgag
     5101 gttcatcgat aaccacgatc atggcaagct gagggttgtc tgcgggcaca tacccagtga
     5161 aggacgcaac aaatcgagag gccgaatagg tacctgttcg tgggtcgatc ttttgggccg
     5221 tacc
//
LOCUS       DASTWE010000398         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_980739, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000398 DASTWE010000000
VERSION     DASTWE010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980739"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..846)
                     /locus_tag="VFT30_04255"
     CDS             complement(184..846)
                     /locus_tag="VFT30_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091871.1"
                     /translation="MKKLLFILLLMFALFEISSAQGTQDRSAVKCAADSPERRGEEGC
                     TILASRPLSGAMTGTIYWHIDRFNSLEDAKKAAGPNGVAAEAHSGVWLMTVEGKTKDH
                     HGGRHVALIGPLNLPAAESYTMRVASSLLKHGASTPVHTHSGPEAWFIVVGDQCLETE
                     KSAHRLKAGKSFVLATDQIHRGRVQSAEMRGALALVLHDSKRPASHDLTESDAPLLTA
                     CR"
BASE COUNT          238 a          311 c          278 g          257 t
ORIGIN      
        1 cgtttccacg aaatcggctt gctccttatg cgtgaggggt gggacactga tgccactcca
       61 acctacacgc atgcgatcgt gttaattgcg ttcgcatagt tgatgacttc aaaaaacgat
      121 ccgagctgga gaccaagcgg atcaacccgc agagtcgttc tgtctttcag cagccgaccg
      181 accttaccta caagctgtca gcagcggcgc atcggattcg gtgagatcat ggctcgccgg
      241 acgtttgctg tcgtgcagaa caagtgctaa ggctcctcgc atttccgcgc tctgaactcg
      301 cccacgatga atctgatcgg tggccaatac gaaggacttt ccggctttaa gccggtgcgc
      361 ggatttctca gtttctaaac actggtcgcc gacgactatg aaccacgctt ccgggcccga
      421 atgagtatgg accggtgtcg aggctccgtg cttaaggagg gatgacgcca cccgcatggt
      481 ataactttcg gcggcaggga gatttagcgg gcctatcaac gcgacatgac gtccgccgtg
      541 atggtccttg gtcttgccct cgacggtcat gagccacaca ccgctgtgcg cttccgccgc
      601 aacaccattg ggaccggcag ctttcttcgc gtcttccagc gaattgaaac gatcgatatg
      661 ccaatatatg gtccctgtca tagctccgga caatggcctg ctagccagga tggtgcagcc
      721 ttcctcgccg cgtcgttcgg gcgaatctgc cgcgcacttg accgcggatc ggtcttgagt
      781 accctgagcc gaagagatct cgaacaatgc gaacatcaac agcaaaatga acaagagttt
      841 tttcatttct tttcccaact tcgagtaatt ttgatctact ggtcgacctt gttaaagacc
      901 accttcccat cgttgacgat cattcaaatg tctttcagcg cctcgatcga tgaaagagtt
      961 ttgtcgccaa tgattccctg atgagctcgt ccaccttcga agatgctgcg tggcaaccac
     1021 cacgtcaggt cactttgtga gcaagcaatt gttcgagcat ccatccacgg agcccgcaga
     1081 gagc
//
LOCUS       DASTWE010000399         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_592729, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000399 DASTWE010000000
VERSION     DASTWE010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592729"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFT30_04260"
     CDS             complement(<1..700)
                     /locus_tag="VFT30_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091872.1"
                     /translation="MSDGTDKNRGLNTTRVFIDRDRKEKEHLTLGQSTLLLGSLLHHG
                     GILRGDGKPLELDAKPGDPRTSKLNHYFPPTGEGLAAVRIFETFPSGEFLPTSAPLEH
                     GTKLKEPEIFQVPKTNVLKAEECMRHGLRATSQLEVLNSASAAYVETLFNVESKEQLN
                     QTLEKIMDLNIKAAHTANNSIKWQTRSVVNSMLLRRDAALKNCKDICDKSGKVAELRS
                     LPFHDCHHLFGDQIL"
     gene            895..1167
                     /locus_tag="VFT30_04265"
     CDS             895..1167
                     /locus_tag="VFT30_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091873.1"
                     /translation="MRRPGSEGALLGEGAWESEGEWCEGEPVGEGEHWPSEAGDAAAL
                     SEGGLAAEAPCDDRWCEGGRCAEGSDASEGKLLAEGGIRGLVPEGS"
     gene            complement(1645..2292)
                     /locus_tag="VFT30_04270"
     CDS             complement(1645..2292)
                     /locus_tag="VFT30_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091874.1"
                     /translation="MKLNSKQINELAELARKSEIRKEQERRKRARQRNVSASQVSTPS
                     STLSTPSKVKKTAKKRSPKTKTKHPKGITLPSSSKKSCTQIPDLSDAMTGKDVNPTKE
                     IPSTFLVISEADDTFVEQNEGESSPSFHEQLLPTQQGMPSDTREMADEQEGSPSSLSL
                     STGLDHDPHYDLTDKITQILKDVLPRFLPQRTSDQAFRDGSIPGVVPARTDKTHS"
BASE COUNT          527 a          625 c          779 g          701 t
ORIGIN      
        1 tcagtatttg atcaccaaac aggtgatggc aatcgtggaa tggcagggat ctgagttctg
       61 ctaccttccc agatttatca caaatgtcct tgcagttctt taatgccgcg tcgcggcgca
      121 gtaacataga gttgacgacg ctccgggttt gccattttat ggaattgttt gcagtgtgag
      181 cggccttgat gttcaagtcc atgatcttct ccaaggtctg gttgagttgc tcctttgatt
      241 ctacattgaa gagtgtctca acgtaggccg ccgatgccga gttcaagacc tccaactgtg
      301 atgttgctct gaggccgtgt ctcatacatt cttctgcttt caggacgttt gtcttcggca
      361 cttgaaaaat ctcaggctct ttaagtttgg tgccgtgttc gagcggggcg cttgtcggca
      421 ggaattcccc tgacggaaac gtctcgaaga tccgtacagc agctaggcct tctcctgttg
      481 gcgggaagta atgatttagt tttgaagttc gtgggtcccc aggtttggca tctagttcga
      541 ggggtttccc atccccccgt aggataccgc catggtggag gagtgagcca aggaggaggg
      601 tagattggcc tagtgtgagg tgctcctttt cctttctgtc ccgatctata aaaacccttg
      661 tggtattaag gcccctattc ttgtctgtgc cgtcggacaa tttctcccga attgcgaagc
      721 tctcaggtaa aatttcgtgt acgagcttcg tgcattgagc taggcttcga agggaccatg
      781 attcgctttt tgcttcgtca ctcttcgaat cgtcccaggt gacccggcga ttggcgaacc
      841 gttgatgatc ctgcatggag agacccgacg aaggtgggat taatcccttg cctggtgaga
      901 cgcccgggga gcgaaggtgc cctgctgggc gaaggtgcgt gggaaagcga aggcgagtgg
      961 tgcgaaggcg agcctgtcgg tgaaggtgag cactggcctt ccgaagccgg cgatgccgct
     1021 gcgttgagcg aaggtggttt ggcagccgaa gctccctgtg atgatagatg gtgcgagggt
     1081 gggcgatgcg ccgaaggtag tgatgcctcc gaaggcaagc tcttggccga aggtggcatc
     1141 cgtggtttgg tacccgaagg tagttgatct tccttctccg gggatggtga ccttgggttg
     1201 tcgacaaaaa tcactggttg ggaaacggtg acgtcggtgg cttgatccgg ccacggttgg
     1261 tccgaatggt catacaatgt tgcaccaaat tcgtttttga agacgttcat tctctgtctt
     1321 ttgcgtgttg attcttctgt ctcagtgaga agacgttttc gagggtcggc aaacggattc
     1381 aattttctag tcaaccttga cggcatgtca gcctcgtacg cacgtttcga taccggcgcc
     1441 ttgtaaatcc tccatgccgg acgaaggcga gtgttgtccc gtataagcga aggtgtgcac
     1501 gggacgtctg catgttgttg cgacgatggt ggatccgggc tagccggcgc gtcgtcgact
     1561 gcttggccga tctcctcccg tcgcgggcgg tgcggcagag tcaatcccgc ggcgagtgtg
     1621 gccgtgcggg ccaggagcac ttcttcatga atgtgtttta tcggtcctgg ccgggacaac
     1681 accggggatg gatccatccc gaaaagcctg atccgatgtt ctttgtggca agaatctcgg
     1741 tagaacatct ttgaggatct gtgttatttt gtccgttagg tcataatgag gatcatgatc
     1801 taggcctgta gaaagtgaga gcgatgaggg tgagccctcc tgttcgtcgg ccatctccct
     1861 ggtgtccgaa ggcatacctt gctgtgttgg gagaagttgt tcgtgaaacg agggtgaaga
     1921 ttcgccttcg ttctgttcga cgaaggtgtc atccgcttcc gatatcacca ggaaggtgga
     1981 cggaatttcc ttcgttgggt tgacgtcctt cccggtcatc gcatcggaca ggtccgggat
     2041 ctgggtgcaa gattttttag aagacgatgg cagagtgatg ccctttgggt gcttcgtctt
     2101 tgttttcggc gaacgcttct tggccgtttt cttcacttta ctcggtgtgg aaagcgtcga
     2161 actcggggta gacacctgcg aggccgaaac gttccgttgt cgagcccgtt tacggcgttc
     2221 ttgttcctta cgaatctcgc tctttcgagc cagttcggcg agttcattga tctgcttcga
     2281 attgagcttc acacaatccg tgcaagggtc ttcgatgatc ttgcatcccc tgcaagaacg
     2341 acaaaaaggg tgcggattac gttcacttct gatgtgagtg caaactctgc cggttttcga
     2401 ccatggctga gggagatctt ggcgagccgg tggttctttg ttcggcgacg ccatcttgag
     2461 aaaacgtccg ttggcacgaa gccgttggca cgaagcaaat tggacgatcg gagcactttg
     2521 taaggtgatg cacactcaaa aaacacccaa atgctgcaca agcaaagcac aacttcgttc
     2581 caaaggaaat cggatgaact aaaacagcta caacgggcgg gaacggtcca cg
//
LOCUS       DASTWE010000400         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_398944, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000400 DASTWE010000000
VERSION     DASTWE010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398944"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..532
                     /locus_tag="VFT30_04275"
     CDS             161..532
                     /locus_tag="VFT30_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091875.1"
                     /translation="MASILVIDDEDSLRAALRRNLQKAGYHVIEANEGRQGLQQLASF
                     PVNLVLIDIFMPGKEGLETIRELRRSYPNIPVIAMSGGGSKGMADVLGVATLLGARRT
                     LLKPFTGQELLEAVQHELAKV"
     gene            625..2703
                     /locus_tag="VFT30_04280"
     CDS             625..2703
                     /locus_tag="VFT30_04280"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091876.1"
                     /translation="MTLNPYRLGELLRRLFRSRSQAVWHQNPLRQGGKVIGVILVSFV
                     LAAVVLFWHLDKLGETLAQTLVLQGADINVAVIEEVRKYYSSNVVARLAGKEEIEVTH
                     DYAMKTHAIPLPATMSIELGKLLRDQMPGAEMRLYSDYPFPWRKEGGPRDAFEREALT
                     WLRDYPDKPYYRFEGIAGDRYLRYAKADVMTADCLGCHNNHPDSPKRDWHEGDVRGVL
                     EVVRPISGSAILIQKELQGMFGLFAGVSLLGLGGMGVAMHRIRRAAVTLGDLVRMRTA
                     ELEAAKEQAEHTAKAKAKILCTVQAFFIGLDQQGIVTEWTLVAERLFGISLVDAIGRP
                     FTSLPIQWNWEKIREACEQSRTALTQVVLDKFEMITQEKQVLYIRLTFTAAVEDQDVS
                     ITVMGEDVTDRLRMEHELVSAQKLESIGHLAAGIAHEINTPIQFVGDNIHFLDTSFTD
                     INRVLDAYRQVFVATKSEACSNELLDACEAAIEAADLDYLLTEIPKAVGQSSEGIHRV
                     AKIVRAMKEFAHPGGEEKTAVDLNKAIESTVTVARNEWKYVADMTTDLAPDLPLVPCL
                     LGEFNQVILNMIVNAAHAIGEVVKDSGQKGLISIASHRVGECVEIRLTDTGAGIPEEI
                     RNKIFDPFFTTKEVGKGTGQGLAIARSVVVDKHRGAITVESQVGKGTTFIIRLPITDP
                     CATSSMEKAA"
     gene            2785..>3669
                     /locus_tag="VFT30_04285"
     CDS             2785..>3669
                     /locus_tag="VFT30_04285"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091877.1"
                     /translation="MKNILFVDDEPVLLDGLRRSLRSMRNEWTMTFADGGEEALKALE
                     QTPFDVVVSDMRMPKMDGAQLLNEVQRRYPHIVRIVLSGYSDKEMILHSIRATHQFLA
                     KPCEPEQLKTTIRRACALQDVLKNEALRALVTGMSAIPSLPAILLEIKQEAESETSSL
                     KAIGKIIVKDMGLTAKILQLANSAFFGVRGPVSTVEQAVNFLGLETVQALILVAHVFS
                     QFSAKHLSTFQIARLWEESLETGALAREIAKVEGCTPFETEQAYTAGLLHDMGILVLA
                     ANCADQFSAILAEAAAKRG"
BASE COUNT          872 a          912 c         1072 g          813 t
ORIGIN      
        1 aggccgatat ttggtcgaat aggttatctc ggaagattga ggcccctagg aaaacaaacc
       61 cgaaaggtgt tcggttcgcc tgcacttcag ccctgagccg ttactaacgg tgcggctgga
      121 gagggaacaa attggttgtt caaagcatcc ggatgagttc atggcatcga ttcttgtaat
      181 tgacgatgag gacagtcttc gtgccgccct acggagaaat ctgcaaaagg ccggctatca
      241 cgtgatcgag gccaatgaag gcagacaagg cctgcaacag ttagcgagtt tcccagtgaa
      301 tcttgtactg atcgatatct tcatgcccgg aaaagagggg ctggaaacca ttagagagct
      361 ccggcgatcg tatccgaata tcccggttat tgctatgtcc ggtggaggat caaaagggat
      421 ggccgatgta ttgggtgtgg caacgctcct tggcgctcga cgcacgctgc tcaaaccatt
      481 cacagggcag gaattgctcg aggctgtgca acatgaactg gcgaaggtct agagcatcgg
      541 gacttctgtg tcgcgtttta gctcattgag ggcttctgag agagacgtcc gtcaggtcat
      601 tgtcttgcac aatcggtcaa aatcatgaca ctaaaccctt atcgtctcgg ggaattgctg
      661 cggcgtctct tccgatcacg gtctcaggcg gtatggcatc agaatccatt gcgacaagga
      721 ggaaaagtca tcggtgtgat attggtgtca ttcgtactgg ctgccgtcgt gctcttttgg
      781 catttggaca aactggggga gaccttggcc caaactcttg ttctccaggg ggcagacatc
      841 aacgtcgccg ttatagaaga agtgcggaaa tactactcgt cgaacgtcgt cgcacgtcta
      901 gcagggaaag aagagataga agtgacacac gattatgcga tgaagactca cgcgattcct
      961 ttaccggcta cgatgagcat cgaactgggg aagctcctgc gtgatcaaat gccgggggcg
     1021 gagatgagac tctatagcga ttacccgttt ccttggcgca aggagggtgg cccccgcgac
     1081 gcctttgagc gagaggcatt gacctggctg cgagactatc ccgacaaacc gtattaccga
     1141 tttgaaggaa tcgcaggtga tcggtattta cgctatgcca aagcggatgt catgacggca
     1201 gattgcctgg gctgtcacaa caatcatccc gacagtccca aacgcgattg gcacgaaggt
     1261 gatgtgcgag gagtgttgga agtagtccgg cctatttcag gaagcgccat tctcatccag
     1321 aaagaactgc aagggatgtt tgggctcttc gcgggcgtca gtttattagg tctgggaggc
     1381 atgggcgtgg ccatgcacag gatcaggcgt gctgcggtca ctctggggga tctcgtccga
     1441 atgcggactg cggaactcga ggcggcaaag gagcaggccg agcatacggc aaaggctaag
     1501 gcgaaaatcc tctgcacggt acaggctttt tttattggac tggatcagca aggaatcgtc
     1561 acagaatgga ctctggttgc agagcgactc tttgggatct ccttggtcga tgcaattggc
     1621 cgtcccttca cttccttgcc catccagtgg aactgggaga agatccgtga ggcctgtgaa
     1681 cagtcgcgaa cggcgctgac gcaggtggta ttggacaagt ttgagatgat cacgcaagag
     1741 aaacaagttc tctacattag gctgactttc acagcggccg ttgaagacca agacgtgagt
     1801 atcaccgtga tgggagaaga cgtgaccgat cggcttcgta tggagcatga actcgtctcc
     1861 gctcagaagc tggaatcgat cggccatctg gcggcgggta ttgcacacga gattaatacc
     1921 cctattcaat ttgtgggcga taatattcat ttcctcgata cctcgttcac ggacatcaac
     1981 cgagtgttag acgcctatcg acaggttttc gtggcgacca agagcgaagc ctgttcgaac
     2041 gagttgctcg atgcctgtga ggcggctatc gaggcggcag acctggacta tttgctgacc
     2101 gaaattccga aggcggttgg acagtctagc gagggtatcc accgggtagc caaaatcgtg
     2161 cgggcgatga aagagtttgc ccatccgggt ggagaagaga aaaccgcagt ggatctcaac
     2221 aaggcaatcg agagtactgt gacggtcgcg cgcaacgaat ggaagtacgt tgccgacatg
     2281 acgactgacc tggcgccaga tcttcccttg gtgccgtgcc tactcgggga gttcaaccag
     2341 gtgattctca atatgatcgt caatgccgct catgcaatcg gcgaggtcgt caaggattcc
     2401 ggtcagaaag gacttatttc catcgcgtcc catcgtgttg gcgagtgtgt cgagatccgt
     2461 ctcacggata ccggtgcagg cattccggag gaaattcgaa acaaaatctt cgacccgttc
     2521 tttacgacaa aggaagtcgg caaaggaacg ggacagggct tagccattgc ccgttccgtg
     2581 gtagtagata agcaccgcgg tgcgatcact gtcgagagtc aagtgggcaa aggaaccacg
     2641 ttcatcatcc gcctcccaat cacggacccc tgtgccacga gttccatgga gaaagccgca
     2701 tgaatcctgc cgatttgcct gccgcattca tgcgatacgg acaatgcctg atcaggcggg
     2761 ctccactgaa gaaagagact cgcgatgaaa aacatcctgt tcgtggatga cgaaccggta
     2821 ctcttggatg gactcaggcg ttcccttcgc tccatgcgaa acgaatggac catgacgttt
     2881 gcagacggcg gagaggaagc attgaaggcg ctggaacaga ctccgttcga cgtggtcgtc
     2941 tcggacatgc gcatgccgaa gatggacggt gcccaactgt tgaatgaagt ccagcgacgc
     3001 tatccacaca tcgtcagaat cgttctgtcc ggatattccg acaaggagat gatccttcat
     3061 tcgattcgcg ccacccatca gtttctcgcg aaaccttgcg aaccggagca gcttaagacg
     3121 acgatccggc gcgcatgcgc gttacaggat gtgctgaaga acgaggctct ccgcgcgttg
     3181 gtgactggta tgtcggcaat tcccagcctc ccggcgattc ttctggagat caaacaagag
     3241 gcggagtcgg aaacgtcttc gctcaaggcc atcggtaaaa ttatcgtcaa agacatgggc
     3301 ctgactgcaa aaatcctcca gctggcgaac tcggcgttct tcggtgtgcg gggacccgtc
     3361 tcgactgtag agcaggctgt aaactttctc gggttggaaa ccgtccaggc gctcattttg
     3421 gtcgctcacg tcttttcgca attctcggca aaacacctgt caactttcca gatcgcccgt
     3481 ctgtgggagg agagtttgga aacgggtgct cttgctcgtg agattgccaa ggtcgaaggg
     3541 tgcacgccat tcgagaccga acaggcttat acggcgggtt tgcttcatga tatgggcatc
     3601 ctggtgttag cagcgaactg cgcggatcag ttcagcgcca ttctcgccga ggctgcagcc
     3661 aagagggga
//
LOCUS       DASTWE010000401         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1110060, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000401 DASTWE010000000
VERSION     DASTWE010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110060"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFT30_04290"
     CDS             complement(<1..695)
                     /locus_tag="VFT30_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5091878.1"
                     /translation="MKHSSARAYDRFASYKLMLLLVVGFLLTYLATAPASAAAQDRPS
                     QLRILTYNLLHDGPWSGFFENGTHLKERLDMTIQELRRLQPDIIAFQEASDSRRHGNV
                     PQRIADALGYHIVFAPATERIFGIGSLDRLIISVLGFKEGPAILSRYPIGASQVYDLP
                     RCRYRLDPRILLRAEIDIPNGPVQVFSAHTSKGDECQLQRVGELFRAHRGKGQAILMG
                     DLNTGEQSSVLTG"
     gene            935..1087
                     /locus_tag="VFT30_04295"
     CDS             935..1087
                     /locus_tag="VFT30_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091879.1"
                     /translation="MVKQPETAPHPDSVVTPPVIDPKMAIDPEAKTEEERHPTLPPEQ
                     PSSPKK"
BASE COUNT          271 a          307 c          304 g          241 t
ORIGIN      
        1 cacccggtga ggaccgaaga ttgctcaccg gtattcaagt cgcccatcaa gatggcttgt
       61 ccctttcctc gatgtgctcg gaacagttct ccgactcgct gaagttggca ttcgtcgcct
      121 ttcgaagtat gcgccgaaaa gacttggacc ggcccattag gtatgtcgat ctcggcacga
      181 agcagtatgc gagggtcgag ccgatagcgg caacgaggca ggtcgtagac ttgcgaggca
      241 ccgatcggat atcggctcaa gatggccggc ccctctttga accctaagac cgaaatgatc
      301 agtcgatcca aggagccgat gccgaagata cgctcagtcg cgggagcgaa cactatatga
      361 taccccagcg catcggcgat ccgttgcggt acgttgccat gtcttcggct gtccgacgct
      421 tcctgaaagg caatgatatc aggctgtaaa cgccgcagtt cttgaatggt catgtcgagt
      481 cgctctttga ggtgggtgcc gttctcgaag aatcccgacc atggcccgtc gtgtaggagg
      541 ttgtaagtca gaatgcggag ttgggaaggc cgatcttgag ctgcggctga tgctggtgcg
      601 gtcgccaggt aagtcagaag aaaacctacg actaacaaca acatgagttt ataggaagca
      661 aaacggtcat aggctctagc agatgagtgt ttcacggtgg aacctataat tcatacacat
      721 ccggggaatc tccgagtagg cagatatgcg tgtcatccgc tatcgttagg ttatgtggcg
      781 atggctgttc attatactcc tcactgtatg tggttcaccc gtgctagggg ctgatatcac
      841 gccgccgtcc aaatcacctc cgccgataac gccgcaagat ccctcaaccg ataaatcgca
      901 accggataat cccccgagca cgcgagaccc aggcatggtg aaacagccag agacggctcc
      961 tcatccggat tctgtcgtga cgccgcctgt tatcgacccc aaaatggcca ttgatcccga
     1021 agccaagact gaagaggagc ggcatccgac gcttccgcct gaacagccct catcgccaaa
     1081 aaaataacgg tcgacatgct atgtcaatcg ttcctgatgc caa
//
LOCUS       DASTWE010000402         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_851301, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000402 DASTWE010000000
VERSION     DASTWE010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851301"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="VFT30_04300"
     CDS             complement(<1..1874)
                     /locus_tag="VFT30_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5091880.1"
                     /translation="MGLKPATNPDVEAATIELSIDGKSVTARDGVSLYDVIAMTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMVVRTEGEDLFLARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSPVLDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVAKGDLALNCEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKKTTTTCNYCGDGCQLYLETKDEEVVRVTSPLSWKNKWGDRSETAKGH
                     GGICVRGRFGFEYLDSKSRLTQPLVREGGQLVQKPWLETMHLLVDRFTEIKGKYGADA
                     IGGLVTARCTNEELYLFQKLMRSGFGTNQLDSSARYGHMNFVLASKHAVGLGRTPNDW
                     EDLTKAKAIILIGSNITETNPLTAVRIKEAMRVYKAQVVTLDSAITNIAKLASHPFLI
                     KPGTEGAVIDGLVKATVDQNLVDEETVGKHPQAFAALKSAVGNVSLEQLAAQTGFPVE
                     AFREIAAVFAESPRSIILCAEGIVRRTNGYQNVLKLMDLAWITGKLGRAGCGVNTVTE
                     EPNEQGAVDMGAAPEFFPGQARFDDPAVRDRFAKAWETSLPPVGSGAHLIEILKRCKS
                     G"
     gene            complement(1973..>2648)
                     /locus_tag="VFT30_04305"
     CDS             complement(1973..>2648)
                     /locus_tag="VFT30_04305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960674.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU5091881.1"
                     /translation="LIDKLKQFLDYFPPKIDEYQVFLERNRIWLGRTKGVAVISAEDA
                     VNFGLSGPVLRGSGVDYDLRKYEPYSAYPKCEFSVPVGKNGDTYDRYWIRVMELYESV
                     KIIRQCLEQMQEGPIMADVPSVTLPPKERVFTNLEAMIQQFKLFSQGFDAPPGEIYCG
                     TEAHKGELGFYIVSTGGGKPYRLKIRAPSFVHMGAFDHMARGYMISDACTIFGTYDVV
                     MGECDR"
BASE COUNT          595 a          794 c          621 g          638 t
ORIGIN      
        1 ccactcttgc accgcttcaa gatttcgatg agatgtgctc ctgatccgac agggggaagc
       61 gacgtttccc atgctttggc gaaacggtcc cgaactgctg ggtcatcaaa ccgagcctga
      121 cccggaaaaa actcaggtgc cgcgcccata tcaacggccc cttgttcatt cggctcctcc
      181 gtcaccgtgt tcacaccaca acctgctcgt ccaagcttgc cggtgatcca ggcaagatcc
      241 atcagtttta agacgttctg ataaccgttc gttcgcctga caatcccttc cgcgcacaag
      301 atgatcgacc tcggcgattc ggcaaaaaca gccgcaatct cccggaaggc ttctacggga
      361 aagccggtct gagccgctag ctgctccaag gaaacattcc ccaccgcgct tttgagcgct
      421 gcaaacgctt ggggatgttt cccaacagtc tcttcatcca ccaaattctg atcgacggtc
      481 gccttgacca acccatcgat cactgcaccc tctgtcccag gcttgatcaa aaacgggtgt
      541 gatgccaact tcgcaatatt cgtgatggcg ctgtccagcg ttaccacctg agccttgtag
      601 acccgcatgg cttctttgat tcgcacggca gtcaatgggt tggtctcggt aatattggag
      661 ccgatcaaaa tgattgcttt cgctttcgtc aaatcttccc aatcattcgg cgttctccca
      721 agccctacgg catgtttgga cgcgagcaca aagttcatat ggccatagcg agcactacta
      781 tcgagctggt tggttccaaa accgcttcgc atgagcttct gaaacaaata cagctcttca
      841 ttcgtacagc gggccgtcac caggccaccg atggcatcgg caccgtattt acctttgatt
      901 tcggtgaatc ggtctacgag caggtgcatg gtttccagcc atggcttctg caccaactgc
      961 cccccctcgc ggaccagggg ctgtgtaagc cggctcttac tgtccagata ttcgaaccca
     1021 aaacgtccgc gtacgcagat cccgccatgc cccttggctg tttccgatcg gtctccccac
     1081 ttgttcttcc acgataacgg ggaggtgact cggaccacct cttcatcttt cgtttccagg
     1141 tagagctggc aaccgtctcc gcaataattg caggtagtgg tcgtcttctt catctgccac
     1201 ggtttataca gatacttgga aaacttattc gtgatggcgc caaccggaca gaccgctaaa
     1261 caatctccgc agaattcaca attgagggcg agatcgccct tggccacgac ctggttgaat
     1321 ccacccttct tcatgaactg caaggcatcg atcatcaaca catccttgca aacattgatg
     1381 cattccgcac aggcgataca gcgattcatg ttgaagtcga gcacgggact gcgcgtatct
     1441 tcgggaatga atttctgctt ggcattggcc agatttgtca cgccatgttg gaaagccata
     1501 tcttgaagtt cacaatgtcc gtccgcatcg cacaccgggc agtcgagagg atgcacagac
     1561 aggtgtttct caacggcttt cttccgggcg aggaagagat cctctccttc tgtccggacc
     1621 accattcccg ccgccgcctt ggcggtacac gaccgaacgg gagctttctt cccctcttgc
     1681 atcaccaggc acatcccgca ggaaccgaat gggtcaaatg tatagtgata gcacatggcc
     1741 gggatgatct tgcctgtcat tgcgatgacg tcgtataacg acactccgtc ccttgcagtg
     1801 acagatttcc cgtcgatgct caattcgatc gttgccgctt caacgtctgg attagtagcc
     1861 ggcttcaagc ccataattct tctccgtgaa agggaaaacg aaagggaaaa catgaaccat
     1921 aaaacggaac cctgatttta cctttcactt tcgcatctcg cgttccacat tactatctgt
     1981 cgcattcgcc catcacgaca tcgtacgtcc cgaagatggt acaggcatcc gagatcatat
     2041 accctctggc catatgatca aacgcgccca tatgcacgaa cgaaggggcg cgaatcttca
     2101 gccgataggg cttccctccc cctgtactga cgatgtaaaa acccaattcc cctttgtgcg
     2161 cctctgtgcc gcagtagatt tctcccgggg gagcatcgaa cccctgcgag aagagcttga
     2221 actgttggat catcgcctct aggtttgtaa aaacccgttc tttcggcggg agtgtcacgc
     2281 tgggaacatc cgccatgatg ggtccttcct gcatttgctc caggcattgt cgaatgatct
     2341 tgacgctctc gtagagctcc atcacacgaa tccagtagcg atcgtacgta tccccgtttt
     2401 ttccgaccgg cacgctgaac tcgcactttg gatacgcgct atagggctcg tacttgcgaa
     2461 ggtcgtagtc cacgccagac ccgcgaagaa ccggtccgct cagtccaaaa ttaaccgcat
     2521 cttcggcaga gatcacggcg actcccttgg tgcgcccaag ccaaatgcgg tttctctcga
     2581 gaaacacctg atattcgtcg atcttcggcg ggaaataatc caggaactgt ttgagcttgt
     2641 cgatcaac
//
LOCUS       DASTWE010000403         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_334538, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000403 DASTWE010000000
VERSION     DASTWE010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334538"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..565)
                     /locus_tag="VFT30_04310"
     CDS             complement(98..565)
                     /locus_tag="VFT30_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091882.1"
                     /translation="MSTSARIVIPLVVVLLTLIGANTPILVCAEESTSIQQILEEPRV
                     YHLRHATLRGIVRDVQPLAPYTVPNGDNCYGAYLFRLEDDEATIPVAVLGICGRPIVR
                     DPEVEEGDRVEVSVTIQAPSHGGYYLSFKGLKVATEQEGIVQAVADRILPIVE"
     gene            677..>1663
                     /locus_tag="VFT30_04315"
     CDS             677..>1663
                     /locus_tag="VFT30_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5091883.1"
                     /translation="MVNERRLRSFVKESRPRFEDLLGQMVEVPSISMDSSRSADMRRI
                     AELAKQYLVGMNAEVQLVETGGYPIVSGGWITGPRYPTVTIYNHLDVQPAQEPEWKQA
                     PFAFKKENGIYRGRGATDDKGPALTAMFGARYAIEQGWPINIRFLWELEEEIGSPHFA
                     AGLRNRSAIPRPDSVVVSDTIWIAKGRPAVPYGLRGLLGARLILRTGEKDAHSGVTGG
                     AARNPLAELMEVANTCVDAKTGKVKIPDFYSDVVELSTKEINDFLKSGFEVRRFKEAY
                     GFRSLRVQDPAEVMRRIWASPTFEIHGLSGGYQGEGVKTVVPGHAELKVSMRL"
BASE COUNT          379 a          403 c          483 g          398 t
ORIGIN      
        1 ccgaacctat gatgacgaat tcgacggggt ttctgccgcg cgaattagtt acacggcatc
       61 tctatagcat ttctgcggat gtattgaaga gaaagaccta ctccacgatc gggagaatac
      121 ggtcggccac tgcttgcacg attccttctt gttcggtcgc cactttcagt cccttgaaac
      181 taagatagta ccctccgtga ctgggtgcct gaatcgtgac actcacttcg actcgatctc
      241 cttcctcgac ttccgggtcg cggacaattg gtctgccaca aatgcccaac actgctacgg
      301 ggatggtcgc ctcatcgtcc tcaagacgga acaagtaagc gccgtaacaa ttatcgccat
      361 taggcactgt gtagggtgcg agcggttgca catcccgtac aatgcctcgc aacgtggcat
      421 gccgaagatg atagacccgc ggttcttcga gtatctgctg gatgctggtc gattcctctg
      481 cgcacaccag tatcggtgtg tttgctccta ttaatgtaag cagaacgaca acgagaggga
      541 tgacgattcg cgcagaagtg ctcatggtgg atgaaaggca tcttatcatc attcttcgtt
      601 ggaacccgga ctgctttctc ttgtctggca cgatcgctat aatcccccct cttttggtca
      661 aaggaggatc actcgtatgg tgaatgagcg gcgactgagg agctttgtaa aggagtcacg
      721 tccaaggttt gaggatctct tgggacagat ggtagaggtg ccgtctatca gtatggattc
      781 atcccgttcg gctgatatgc gacgcatagc tgaacttgcg aagcagtact tggttgggat
      841 gaatgccgag gttcaattgg tagagaccgg tgggtacccc attgtttccg gcgggtggat
      901 aacagggcct cggtatccga ccgtcacgat ctacaatcat ctggacgtgc agccggctca
      961 ggaaccggaa tggaagcagg cgccgtttgc ttttaagaaa gaaaacggaa tttatcgggg
     1021 gcgaggagcg accgatgaca agggcccggc tctcacggcg atgtttggtg ctcgatatgc
     1081 tatcgaacaa ggttggccga tcaacatccg attcttgtgg gaattagaag aagagatcgg
     1141 gagcccgcat tttgccgccg ggcttagaaa ccggagcgcg attccgcgac cggattccgt
     1201 ggttgtatcg gatacgatct ggattgccaa agggcgacct gctgtgcctt atggcttgcg
     1261 aggtttgctc ggcgcacgtc tgatcctgcg cacaggtgag aaagacgcac attcaggcgt
     1321 gacgggggga gcagctcgta acccgttggc cgagttgatg gaggtggcga atacctgcgt
     1381 tgatgccaag accggcaagg tgaagatccc tgatttctat agtgatgtgg tcgagcttag
     1441 caccaaggaa atcaatgatt tccttaaatc aggctttgaa gtaagacgtt tcaaagaagc
     1501 ctacgggttt cgatcacttc gggtacagga tccggcagaa gttatgcgac ggatctgggc
     1561 atccccgacc ttcgaaattc atggtctcag cggaggatac cagggtgagg gtgtcaaaac
     1621 tgtggtgccc ggtcatgcgg agctcaaggt cagtatgcgc ctt
//
LOCUS       DASTWE010000404         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1013324, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000404 DASTWE010000000
VERSION     DASTWE010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013324"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFT30_04320"
     CDS             complement(<1..299)
                     /locus_tag="VFT30_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5091884.1"
                     /translation="MDLEKLKAFIVVAEELSFRKSAEILGMSQPPLTRRIASLEHELD
                     AKLFERTTRRVRLTGAGVLLLREAREIASAISRIESDVRAIGKKTTGILKIGFSRA"
     gene            459..1172
                     /locus_tag="VFT30_04325"
     CDS             459..1172
                     /locus_tag="VFT30_04325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5091885.1"
                     /translation="MPQENKMAREFFDKQASETYDQRIQPIAPIIDNLHFLIRLILKD
                     LARDANILCVGVGTGTEILELAQAFPYWRFTGIDPSASMLDVCRRRLQKSSLLTRCEL
                     HHGYLSDLPKIEQFDAILCLLVAHFIKDSQERQALFNGMAARLKVAGYVIHAEISYEL
                     SAAEFPAIGEKWKALHRVAGASEERVANILKTLQEDVAVLPPASTEHLLRSSGLPMPI
                     PFFQSLLIRAWYAQKTQNR"
BASE COUNT          308 a          313 c          307 g          287 t
ORIGIN      
        1 gctctcgaaa atccaatctt aaggatgccg gtcgtctttt tcccgatggc acggacatcc
       61 gattcaatcc ttgaaatagc cgacgcaatc tcccgtgctt ctctcagaag caggacgccc
      121 gctccggtca accggacgcg tcgcgtggtt ctttcaaaca atttggcatc cagttcatgt
      181 tccaaagaag caatacgcct cgtaagcggc ggctgagaca tcccgagaat ctcagcactt
      241 tttcgaaagc ttaattcctc ggcaacaaca ataaacgctt tcaacttttc aaggtccatg
      301 ctgcattatt actttttgag tatcaataga tcaatgaatg gggaacgatc gaagtcggcc
      361 tccatgctat tatcgtggcg cggaaagaat tcggacggat tgcatgtacg gagcttcggc
      421 aggaacctag aacgccgagt cttacgggat gagaagaaat gccacaagag aacaaaatgg
      481 cgagggagtt ttttgataaa caggcttcgg agacctatga tcaacgtata caaccgatcg
      541 ccccgatcat cgataacctg catttcttga tacgtctgat cctgaaagac cttgcccgtg
      601 acgcaaatat tctctgcgtt ggagtcggaa ccgggacgga gatcttggaa ttagcacaag
      661 cgtttcctta ttggcgattt accgggattg atccctccgc atcgatgctc gatgtctgcc
      721 ggaggcgcct gcaaaagagc agcctcttga ctcgttgtga gttgcatcat ggatatctgt
      781 cggacctgcc gaagatcgaa cagtttgacg caattctctg tctgctcgtg gctcatttca
      841 tcaaagatag ccaagaacga caggccctct tcaatggaat ggcggcacgg ttgaaggtgg
      901 ccgggtacgt aattcatgca gaaatcagct acgagctgtc ggcagcggag tttcccgcca
      961 tcggtgaaaa atggaaagcc ctccacaggg tagcgggcgc atcggaagaa cgggtggcga
     1021 atattctaaa aacattacaa gaggacgtcg cggtgttgcc gcccgcctca acagaacatt
     1081 tgctccggag tagcgggctg ccgatgccga tccctttctt ccagtcactg ctcatacggg
     1141 cttggtacgc ccaaaaaacg cagaaccgtt gatggcgatc ttcgctgcga cctccgtgaa
     1201 aatcgacttt cgatt
//
LOCUS       DASTWE010000405         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_980891, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000405 DASTWE010000000
VERSION     DASTWE010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980891"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(639..>1107)
                     /locus_tag="VFT30_04330"
     CDS             complement(639..>1107)
                     /locus_tag="VFT30_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091886.1"
                     /translation="KGNTQKKLRPLSRAELSGTGNFYISYLTAFPPPVQNISTALFSL
                     QAIVFRCCDRAIADLLEMQQRLFPALQIGKSCPSDLGHFGGWVEIHQFLVKLLGMRDI
                     TLVFFEGGRFKQLFSFLIGATDQHQTEGTEYKSCGYHGDPFQASLRRDTLVFV"
BASE COUNT          274 a          285 c          304 g          244 t
ORIGIN      
        1 tggcggcacg cctgcgccac catctcgaga ccgagcggtt ccaggcgtag gtagatcttg
       61 gtgtacatga gcggaccggg atggactgcg aggaatttca ttggcgcaca cctcctcgtc
      121 tatagcacgc tgggccggaa gagaacctgt tcggtgtacg aaggactggt ccggccgcct
      181 ggttaatcag tgatgtgaca atcgacgcgg gaagggattt tgacagaaca ggagcagagg
      241 tgcaaccttt taacgcaggc ccatcgaaga ttgaaccgac caagacatct ggcatttgct
      301 cgatcgtatg cttaccataa tggcatgttg tctgctccgc gcaccctgcg tcagcgcatc
      361 atcgatcttc tgaccgatac caggatgacg accgatcaac tggctcggat gctcggtatt
      421 cctgaacggc aggtggagga acacttggct catgtggtaa aaacggtggc tcgagaccgt
      481 tcacgacggt ttgtccttga accgtccatc tgcttggatt gtggatttat gtttcgcgac
      541 cgcacaaaat tgacgcgacc aagccgctgc ccacaatgtc ggagcgaagg gatttctgcc
      601 ccacgctacg gaatcgattc tcttggatca ggatctactc aaacgaacac caaggtgtca
      661 cggcggagag atgcctggaa aggatcacca tgataaccgc acgacttgta ttctgtgccc
      721 tctgtttgat gctggtcggt tgctccgatc agaaagctaa agagctgttt gaaacggccg
      781 ccttcgaaga aaaccagggt aatgtccctc atgccaagca gctttaccaa gaactggtga
      841 atctctaccc atccaccaaa gtggccgaga tcgctcgggc acgacttgcc gatctggaga
      901 gcaggaaata gacgctgttg catttcaagc aagtcggcga tggcacgatc gcaacaccga
      961 aacacgatag cctgcaaact gaacagggca gtggaaatat tttgaacggg tggcggaaac
     1021 gctgtcaggt acgaaatata aaaattgccc gttccactaa gctccgccct cgaaagagga
     1081 cggagctttt tttgtgtatt gccttta
//
LOCUS       DASTWE010000406         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1174603, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000406 DASTWE010000000
VERSION     DASTWE010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174603"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..440
                     /locus_tag="VFT30_04335"
     CDS             96..440
                     /locus_tag="VFT30_04335"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU5091887.1"
                     /translation="MTDRAEDRGQVHDSIHEMVRRIAAQFRPDKIILFGSHARGDARP
                     DSDVDLLIVMPVQGSKRQKATEIDMALADRTVSLDLLVVTPEDFERGRAEIGSVLRPA
                     ALEGQVLYDRAA"
     gene            569..838
                     /locus_tag="VFT30_04340"
     CDS             569..838
                     /locus_tag="VFT30_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU5091888.1"
                     /translation="MTMSRVRVADLKSRLSEHLRKVRAGRSVTVLDRDTPIARIVPYE
                     EQGSSLTVRHPLPGAPSLKRIPLPPPMKLRRDIVELLLEERQGEQ"
     gene            835..1263
                     /locus_tag="VFT30_04345"
     CDS             835..1263
                     /locus_tag="VFT30_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5091889.1"
                     /translation="MIAYLDSSAILRIILGQAHALEEWRSITQGVASALVEVECLRTL
                     DRLRLAEGLSDAEIAVRREAVFRLLESVEIVELTRPVLARASQPLPTALGTLDAIHLA
                     TALLWKDRSGVDLVMATHDEVLATASRASGLRVIGISKSF"
BASE COUNT          263 a          335 c          391 g          286 t
ORIGIN      
        1 accgcgaaag gttatgctcg atgcgatgct cttagcggta cgaaggtgac ggaacgatac
       61 gaaatacggt agattcccgt tcgtaaggag gctctatgac agaccgcgct gaggaccggg
      121 gacaggttca cgactccatc cacgaaatgg tgcggaggat tgccgcccag tttcgcccgg
      181 acaagatcat cctcttcggt tcgcatgcgc gcggcgatgc acgtccggac agcgatgtcg
      241 atttgcttat cgtgatgccg gttcaaggat cgaaacgcca aaaggcgacg gaaatcgata
      301 tggcgctcgc ggaccggacg gtttctctgg accttctggt ggtcactccc gaagacttcg
      361 aacggggtcg ggcggaaatt ggctctgtct tgcggccggc cgcgttggag gggcaggtgc
      421 tctatgaccg agccgcgtga ccagccagga atcatagttc tccaacctcg ctagcgatcc
      481 tggacaaggt gtggaagacc ccgtgctgta accttcactg tcacacccgc cggcggaact
      541 tgcctttgtg accattcatg actatcatat gaccatgagt cgcgtacggg tggcagattt
      601 aaaaagccgg ctgagtgaac atctccgtaa ggtgcgggcc ggacgatcgg tgaccgtttt
      661 ggatcgtgat actcctattg cccgtattgt gccctatgag gagcaaggtt cgtcgctgac
      721 cgtacgacat ccgcttcccg gtgccccctc gctcaagcgt attccgctgc ctccgccgat
      781 gaagctccgt cgcgatatcg tcgagctgct cttggaagaa cgccaaggag agcagtgatc
      841 gcctacctgg attcttcggc gattttacgg attatcctgg gacaagcaca tgcgctggag
      901 gagtggcggt cgattactca aggagtggcg tctgctctgg tcgaggtcga atgtctgcga
      961 actctggatc gtttgaggtt ggccgaagga ttaagcgatg ccgagatcgc cgtgaggcgg
     1021 gaagccgtat tccgattgtt ggaatctgtc gaaattgtag aactcacgag gccggttctg
     1081 gccagagcgt cacagccatt gccgactgcg ctcggtacgt tggacgcgat tcacctcgcg
     1141 acagcgttgt tgtggaaaga tcgcagcgga gttgatctgg taatggctac acatgatgag
     1201 gtattggcga cggcgtcccg tgccagcgga ctacgggtga ttggaatttc aaagtctttc
     1261 tgaccgccct aacat
//
LOCUS       DASTWE010000407         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_173002, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000407 DASTWE010000000
VERSION     DASTWE010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173002"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /gene="tmk"
                     /locus_tag="VFT30_04350"
     CDS             <1..697
                     /gene="tmk"
                     /locus_tag="VFT30_04350"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5091890.1"
                     /translation="IIVEGIDGSGKSTQLQLLHKWLESKGHKVFFTEWNSSELVKETT
                     KRGKKSKSLTPTTFSLLHATDFASRLYHQILPPLKAGMLVLADRYMYTAFARDVVRGV
                     ANEWVRKLYAFAIKPDMAFYFKVPIQVAISRLSRGTRGHFKYYEAGMDMDLSPDITES
                     FRLFQSRILAEYDKIVDECGLLTMDATQDIETQQEHMRTLVEEALRGYKPKRGTYGRR
                     TLFWRRFEVLRSE"
     gene            645..1367
                     /locus_tag="VFT30_04355"
     CDS             645..1367
                     /locus_tag="VFT30_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091891.1"
                     /translation="MADARFFGDGLKYLDPSDLKGKLIAIEGTDGVGRTTHIEMLQEW
                     LEVQGYGVMTTGWTRSNLMSKTIEMAKAGNILDRWSLSLLYATDFADRLEHQIIPALR
                     SGFVVLADRYIYTAFARDLVRSADRKWIRDVFGFALIPDLVCYLRIDVETLALRVIET
                     TGMNYWESGMDLRLGGDLYDSFKKYQSLLIEEFDKMAIEFRFNVVDARKSPEEIQDEL
                     RGYILPVLQNHKPAIDAEMTPS"
     gene            complement(1364..>1654)
                     /locus_tag="VFT30_04360"
     CDS             complement(1364..>1654)
                     /locus_tag="VFT30_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091892.1"
                     /translation="LAAGAKPERLVGLFRTLPSDAVVICVGHEPQLGEVVSLLLCGKA
                     LPNFPLKKAGAARVESEGIINQGQGRLCWWLQPMQLRTIGKRAYGKKRIEQS"
BASE COUNT          409 a          454 c          429 g          362 t
ORIGIN      
        1 gatcatcgtc gaaggcattg atggatcagg caaaagtact caattgcaac tgctgcataa
       61 atggcttgag tccaaaggcc acaaggtgtt ctttaccgaa tggaattcct cggaactcgt
      121 gaaagaaacc accaaacgcg gaaaaaaatc gaagagcctc accccgacga cgtttagttt
      181 gctccatgcc acggacttcg ccagccggct ctatcatcag attctcccgc ccctcaaggc
      241 gggcatgctt gtcctggccg atcgatatat gtatacggcg tttgcccgcg atgtggtacg
      301 cggcgtggcg aacgagtggg ttcggaaact ctacgcgttc gcgatcaaac ccgatatggc
      361 gttttatttc aaagtcccta ttcaagtcgc catctcccga ctctcgcgag ggactcgcgg
      421 ccacttcaaa tactatgaag cgggcatgga catggatctg agcccggaca tcaccgaaag
      481 tttccgactc tttcaatcac gaattctcgc ggagtacgac aagatcgtcg atgaatgcgg
      541 tctgttgaca atggatgcga cccaagacat cgagacgcag caagaacaca tgcggacgct
      601 ggtggaagaa gcgctccgtg gctataaacc aaaacgaggc acctatggcc gacgcacgct
      661 tttttggcga cggtttgaag tacttagatc cgagtgatct gaaaggcaag ttgatcgcca
      721 ttgaggggac cgacggagtc ggccgaacga cccacatcga aatgctgcaa gagtggctag
      781 aggtccaggg gtacggtgta atgacgaccg ggtggacccg ttccaatctc atgtccaaga
      841 ctatcgaaat ggcgaaggcc ggcaatatcc tcgatcgttg gtcgctcagt ctgctctatg
      901 cgacggattt cgccgatcgc cttgagcatc agatcattcc ggctctccga tccggattcg
      961 tcgtcttggc agatcgctat atatacacgg cgttcgcgcg cgacctcgtg cggagcgccg
     1021 accgaaaatg gatccgtgat gtgtttgggt ttgcgttgat tcccgacttg gtctgctacc
     1081 tacgaatcga tgttgaaacc ctggcgctac gagtcattga gacgaccgga atgaactact
     1141 gggaatccgg aatggatctc cgcctcggag gcgacctcta cgatagcttc aaaaaatatc
     1201 aatcgttgct gatcgaagaa ttcgataaga tggcaataga atttcgcttc aatgttgtcg
     1261 acgcgagaaa gtctcccgaa gaaatccagg acgagctccg aggatatatt cttccggtgc
     1321 tgcagaacca caaacccgcg atcgatgccg aaatgacgcc ctcttaagat tgttcaattc
     1381 gtttcttgcc gtacgctcgc ttgcctatgg tccgcagctg catcggctga agccaccagc
     1441 acaaccgacc ctggccctgg ttgattatac cctctgattc gacacgcgca gcgccggcct
     1501 tcttaagtgg aaaattggga agagctttcc cacagagcaa cagacttacc acttcaccaa
     1561 gctgaggttc atgtcccaca catatcacca cggcatccga cgggagtgta cggaacaatc
     1621 caaccagacg ctcaggcttc gcgccggccg ccaa
//
LOCUS       DASTWE010000408         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_625150, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000408 DASTWE010000000
VERSION     DASTWE010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625150"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VFT30_04365"
     CDS             <1..1351
                     /locus_tag="VFT30_04365"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5091893.1"
                     /translation="VEHPDKGAIELDGRPVQVRSPQHSQELGISTVYQEINLCTNISV
                     AENIMLGHEPRRFGSIYWKKMNGMASQAIKRLGIDIDVTQPLGNYSVAIQQMVAIARA
                     LEILSTKVLILDEPTSSLDIHETNLLFDVMRKLKSEGIGIVFITHFLDQVYQIADRIT
                     VLRNGKLVGTFDTDSLPRIELIAKMLGRSVTDLDAMSKARSTLEQKTAEECLAEAKGM
                     GLKRSLEPIDLELHANEVVGLAGLLGSGRTELARLIFGVNTPDTGTLLIKGQKVEHFA
                     PMESLKRGIALCPEDRKAEGIVGDLTIRENIILALQARYGWFKFLNAQKQYEIAQKYI
                     DLLGIRTPSSDQLVKNLSGGNQQKVILARWLVTQPNILVLDEPTRGIDVGAKAEIQKL
                     VLELAKEGMSVVFISSELEEVLRISDRIVVLRDRKKAAEFSSDVSDQTIMHAMAGDL"
     gene            1348..>1530
                     /locus_tag="VFT30_04370"
     CDS             1348..>1530
                     /locus_tag="VFT30_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091894.1"
                     /translation="MKDAPFAYQGKGSIKGIPGLKRIRESRLFWPLATWALILVFDAI
                     FIPGFFKIGIQDGHLYG"
BASE COUNT          371 a          376 c          438 g          345 t
ORIGIN      
        1 cgtggaacat ccggacaaag gcgcgattga gctggatggc cggcctgttc aggtgcgttc
       61 cccgcagcat tctcaggaac tgggcattag tacggtgtat caggagataa acctgtgcac
      121 caacatttcc gtggcagaga atattatgct tggccacgag ccgcggcgtt tcggcagcat
      181 ctactggaag aagatgaacg gcatggctag ccaggccatc aagcgattgg gcatcgatat
      241 cgacgttacc caaccgctgg gtaactattc ggtcgccatc caacagatgg tagcgattgc
      301 gcgcgcactt gagatcttgt cgactaaagt gcttatcctg gatgaaccca cttccagcct
      361 ggatatacat gaaacgaatc tactgtttga cgtgatgcga aaactgaaga gtgagggcat
      421 tggtattgtt ttcatcaccc atttcctgga tcaggtgtac cagattgccg accggatcac
      481 ggttctccgc aatggtaaat tggttggcac gtttgataca gactcactgc cgcgtatcga
      541 gctcatcgcc aagatgctcg ggcgtagtgt aaccgactta gatgccatgt cgaaagccag
      601 atccacgctc gagcaaaaga ccgcagaaga gtgcctggcc gaagccaagg gcatggggct
      661 caagcgatcg cttgagccga tcgatttgga actacacgcc aacgaggtgg ttgggctcgc
      721 gggactgtta ggttctggcc gcactgagct ggcgagactc atctttggcg tcaatactcc
      781 tgatacaggt acgcttctaa tcaaaggtca gaaggtcgaa catttcgcgc cgatggaatc
      841 gcttaagcgt ggaatcgcct tatgccctga agatcgtaag gccgaaggaa ttgtgggaga
      901 cctaaccatt cgcgaaaata tcatcctggc attgcaggcg cggtatggct ggttcaagtt
      961 cttgaacgcc cagaagcaat atgaaatcgc ccagaagtat atcgaccttc tcggcatccg
     1021 cacgccatcc tcggatcaat tagtcaaaaa cctgagcggc ggaaatcagc agaaggtcat
     1081 cctggctcgc tggctggtca cacagccgaa catattagtt ctggatgagc ctacccgagg
     1141 aattgatgtg ggggccaaag cggaaattca aaagctggtg ttggagctcg cgaaggaggg
     1201 catgtcggtt gtattcattt cctccgagct ggaagaagtg ctgcgcatca gcgaccgtat
     1261 cgtcgtgctg cgggatcgca agaaggcggc agaattctct agcgatgtga gtgatcagac
     1321 cattatgcac gcgatggcag gggatctatg aaagacgcgc cgtttgcata ccaaggcaag
     1381 ggatcgatca aaggcatccc gggcctcaaa cgtattcgtg agagccggct gttttggccg
     1441 ctggctacgt gggccttgat cctcgtgttt gacgcgattt ttatcccggg attcttcaag
     1501 atcgggatcc aggatggcca tttatacggg
//
LOCUS       DASTWE010000409         1274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_625161, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000409 DASTWE010000000
VERSION     DASTWE010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625161"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFT30_04375"
     CDS             complement(<1..412)
                     /locus_tag="VFT30_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091895.1"
                     /translation="MNLEDHPTVRRLSSRVQQVENQPPAEDKLDSAWLRRLALDCGAD
                     DAGVVEIARPALDLQHEEILRNYPWTKSLLAFVVRMAREPLRGAPRSVANLEFHRAGE
                     EVNEVAAAIVAKLETRGVRAVNPSMGFPMEMGQHP"
     gene            810..1190
                     /locus_tag="VFT30_04380"
     CDS             810..1190
                     /locus_tag="VFT30_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5091896.1"
                     /translation="MNDRLSRGSKIVPTLFIGKWTVKILYMLKERPHRHGKLRHRLGN
                     ISQRMLTRTLRNLESTGLIARRVTRSKSLAVEYSLTKLGRGFIIPLARICRWIDHHNM
                     ELSAIIRLANRTRSRRNLPQLSKP"
BASE COUNT          312 a          357 c          340 g          265 t
ORIGIN      
        1 ccggatgctg acccatctcc ataggaaagc ccatcgaagg attgaccgca cgaaccccgc
       61 gtgtttcaag tttcgccacg atggctgcgg cgacctcgtt cacctcctca ccggcacggt
      121 ggaactcgag attggcgacc gaccgcgggg ccccgcgcag gggctcgcgc gccattcgga
      181 ccacgaaggc gaggagcgac ttggtccagg gatagttcct gaggatctct tcatgttgta
      241 ggtcgagagc aggacgcgcg atttcaacga cgccggcatc atccgcaccg caatccaaag
      301 caagacgacg caaccaggca ctgtccaatt tgtcctcagc tggtggctga ttttcgactt
      361 gctgaactcg gctggagaga cggcgaacag ttggatgatc ttccaggttc atggcctcag
      421 cctcccgtgc ctgctagagt ccttgttgtt tttcggaggc gaaatttata tattccgttc
      481 ttcttatccg gtacccgtac tgcaacctgt ctgagccaag caaggggcca ggcagacagc
      541 gctgtagttg ctcaattctc cagataaacg tgtacacatc tgtgtgagtc aaaaacaacc
      601 tggcgtcctc ggtcgtcgcg tgaaacgggc ctgagtgcca ctctgttgca aaacacgaag
      661 tccgtcaagt aggcacactg gtgttcccgc ttgcacctaa gactgagtag tctgcgactg
      721 gaccgtggcg ctgcaacgga aggatacaga cacaattcct gcgcggcacc gcacttcaag
      781 gaattctcaa aggagggatc tagggcaaaa tgaatgacag actcagccgt gggtcgaaaa
      841 ttgtgcccac gcttttcatc ggcaagtgga cggtcaagat cctgtacatg ctgaaggaac
      901 ggccccaccg ccacgggaag ttgcgacatc gtctgggaaa catctcgcag cgtatgctta
      961 cgagaacgct tcgcaatctc gaatccacgg gattgatcgc aagacgggtg acgcgatcca
     1021 agtcccttgc cgtcgagtat tccctgacaa aactagggag gggattcatc attccattgg
     1081 caagaatctg ccgttggatc gatcaccaca acatggaact cagtgctatc atccgccttg
     1141 caaatcgaac gagaagcagg cgtaatctgc cgcaattaag caagccttag aaagacgacg
     1201 agataaggag ttcttcttgc tacgtccgac aaaagaaata cgcaacgttt cacaaacctg
     1261 gatcagtcag cccc
//
LOCUS       DASTWE010000410         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_851530, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000410 DASTWE010000000
VERSION     DASTWE010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851530"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1025)
                     /locus_tag="VFT30_04385"
     CDS             complement(<1..>1025)
                     /locus_tag="VFT30_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU5091897.1"
                     /translation="ARGFGAEGIGLCRTEHMFFAEDRIPIMQKMILARTKEEREKYLD
                     QLLPLQKQDFVGLYREMKGYPVTIRLLDPPLHEFLPKREELMVEIAQLELTGNDGAKL
                     EEQRRLLARVEELHEFNPMLGLRGCRLGITMPEITRMQARAIMEAACELAKEGVKIVP
                     EIMIPLVGMVSEMKSQKDLIREVAQETMKRYDVKLSYLIGTMIELPRAAVTAEHIAKE
                     AEFFSFGTNDLTQTTFGFSRDDAAKFIDHYRTVGIMESDPFAVLDREGVGSLMQQAIV
                     GGRTTRSDIKLGICGEHGGDPSSVEFCHQVGLDYVSCSPYRVAIARLAAAHAALAEED
                     MKRASKST"
BASE COUNT          211 a          324 c          251 g          239 t
ORIGIN      
        1 gtggatttgc ttgctctttt catatcttcc tcggccagag ccgcatgggc ggcagccaac
       61 cgggcaatcg ccactcggta aggcgaacaa ctcacatagt ccaagcccac ttgatgacaa
      121 aactccacgg aacttggatc gcctccatgc tcaccgcata tgcccagttt gatgtcggac
      181 ctggttgtac ggcctccgac gatcgcctgt tgcatcagtg atccgacccc ttccctgtcc
      241 aacacggcaa acggatccga ctccatgatg ccgacggttc gataatggtc gatgaacttg
      301 gccgcatcat cccgcgagaa tccaaatgtc gtttgcgtca aatcgttggt cccgaacgaa
      361 aagaactcgg cttccttggc gatatgttcg gcagtcacgg ccgcgcgggg caattcgatc
      421 atcgtcccga tcaagtacga tagtttcaca tcgtagcgtt tcatcgtctc ttgggccact
      481 tcgcggatca gatctttctg cgacttcatc tccgagacca tgcccacgag cggaatcatg
      541 atttccggta cgatcttgac gccctccttc gccaattcgc aggcggcttc catgatcgcc
      601 ctcgcctgca tgcgcgtgat ctccggcatc gtaatgccca atcggcatcc acgaagaccg
      661 agcatcggat taaactcgtg tagttcttcg acccgcgcga ggagccgccg ctgttcttcc
      721 aacttggctc cgtcattgcc cgtcaattcc agctgcgcga tctcgaccat cagctcttcc
      781 cgtttgggca gaaactcatg gagcggggga tcgagcaggc gaatcgtcac gggatagccc
      841 ttcatctctc gatagaggcc gacaaagtct tgcttctgca acggcagcaa ttgatccagg
      901 tacttttcac gctcctcttt cgttcgggcc aagatcatct tctgcatgat cgggatacga
      961 tcctcggcga aaaacatgtg ttcggtccgg cagagaccga taccctccgc gccaaacccc
     1021 ctggc
//
LOCUS       DASTWE010000411         1401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_269712, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000411 DASTWE010000000
VERSION     DASTWE010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269712"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..675
                     /locus_tag="VFT30_04390"
     CDS             52..675
                     /locus_tag="VFT30_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEU5091898.1"
                     /translation="MMERAVATGGQRSTTKRWLFFEGILGDRTANLLEALDGVGYVTT
                     GMSFLAVSIVLFVRAWYVFGISVGSDAVLAVLGLVHDLLLVIILLELFRTTINFLKTK
                     VITLEPFLYICVIASTRRILTTGAQVSYMDELTDLVFNRYLMDLGANVLVVVALIVAI
                     YVSRRASLPVMAEGTETAQYTDRTGKRAESCPQETNGQIVRLKKEYA"
     gene            672..1295
                     /gene="nuoI"
                     /locus_tag="VFT30_04395"
     CDS             672..1295
                     /gene="nuoI"
                     /locus_tag="VFT30_04395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU5091899.1"
                     /translation="MRLRERLAKQYRGFTAWIKLITFYELAVGMKSTLSHLIHYKPIT
                     LQYPHEKRILPDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIAVVSAEVPEEPTK
                     RYAREYSMDMTRCLFCGMCVQACPVDALAMTREYEWAVYDKRDLLLNKQQLLAIGDRA
                     FQIREKRLEFQHPNLAVFNVASSNHPPKVYEFPGEDARHDQTVGSHR"
BASE COUNT          317 a          326 c          412 g          346 t
ORIGIN      
        1 agccgtagca cctgatttgc gaagacagga tgctgtgtag caccaggaga catgatggaa
       61 agagcggttg caacaggcgg acagcgttcg acgacaaagc gatggttgtt cttcgagggc
      121 atcctaggcg ataggacggc caacttgctg gaggccctgg acggggtcgg ttacgtcacg
      181 acgggaatga gtttcctggc ggtcagcatc gtgctgtttg tccgtgcctg gtacgtcttc
      241 gggatctcag tgggatcgga tgcggttctc gcagtcctcg ggctggtgca tgatctctta
      301 ctggtcatca tcttgttgga gctgtttagg accactatta attttctcaa gacaaaagtg
      361 attaccttgg agccgttctt gtatatctgt gtgattgcgt caacgcggcg catccttaca
      421 actggcgccc aagtttcgta tatggacgaa ctaaccgatc tcgtgttcaa ccgatatctc
      481 atggatcttg gagcgaatgt gctcgtcgtg gtggccttga ttgttgcgat ctatgtctcg
      541 cgacgggcat ccctgccggt tatggctgaa ggaactgaga cggctcagta tactgatcgc
      601 acgggcaaga gagcagaatc gtgtccgcaa gagacgaatg gacagatcgt caggctcaag
      661 aaagaatatg catgagactg agagagagat tggcaaagca atatcgcgga tttacggctt
      721 ggatcaagtt gatcaccttc tacgagctcg ctgtgggtat gaagtcgact ctgagccatc
      781 tcattcatta caagccgatc acccttcagt atccacacga gaagcggatt ctgccggaca
      841 attatcgcgg catgctggcc ttgctgcgtt atgacgatgg aaccgagaag tgcgtcggat
      901 gtgatctctg tgaggccgcc tgtccctctc gcgtcattgc cgttgtgagc gccgaagtac
      961 cggaggaacc gacgaagcga tacgcgcgag agtattcgat ggacatgaca cgttgtttgt
     1021 tttgtggaat gtgtgttcaa gcctgtccgg tcgatgccct tgcgatgacg cgggaatacg
     1081 agtgggccgt atacgacaag cgggacctgt tactcaataa gcagcagctg ttggcaattg
     1141 gggatcgtgc gtttcagata cgcgaaaaac ggctggagtt ccagcatccg aatctggcgg
     1201 tcttcaatgt cgcctcatcg aaccatccgc ccaaagttta cgagttccct ggagaagatg
     1261 cccgccatga tcagactgtc ggctctcatc gttgagtcgt ggtgacattg cgtctatcaa
     1321 agtgcttctc catgtaagcg tcccttatct tgattgaaat aggacgatga gaaaggtgtt
     1381 tagagagagc cgataggagg g
//
LOCUS       DASTWE010000412         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1562652, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000412 DASTWE010000000
VERSION     DASTWE010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562652"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..961)
                     /locus_tag="VFT30_04400"
     CDS             complement(320..961)
                     /locus_tag="VFT30_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5091900.1"
                     /translation="MKIALIGATGFVGSAILKEALERGHEVTAIVRHPDELQAHPKLH
                     AKKGDVYSTDEVARLISGHDAVISAFNPGWSDPEIYNHQVKGTESIIKGVKQAGVKRL
                     LFVGGAGSLEVKPGVQSVDLPQFPAEYKQGALATRDALNMLRKETSLEWSFLSPSADL
                     SPGQRTGKFRLGKDQLLKDAKGESRVSVEDYAVAMLDEAERPAHIRQRFTVGY"
BASE COUNT          310 a          440 c          357 g          316 t
ORIGIN      
        1 tttctaatcg gtcgtggtgt gaatcagaac gtgctcgtac tcagaggaac ttccacttca
       61 caaatactgc caccgcagag tgcagcaaaa gatcaatgga aagattcggc agtcttcgaa
      121 aaacgagtac ccggctctcg atacacctat ccacatgttg gccgctcccg acaaacgcgg
      181 caatagccct tgtcatggct gaagtgcccg aattggctaa tagcgttgcg actcccaggc
      241 aacacgatga tgactgccgc gcgcatcgtc cgctccttta ccctcccacc gatggggaaa
      301 gagagggctg ggtatcgatt caatagccaa ctgtaaaccg ctgccgaatg tgggctgggc
      361 gctcggcttc gtcgagcatg gccacggcat agtcctcgac cgagacacgg ctctctccct
      421 ttgcatcctt cagcaactga tcctttccca gacggaactt gccggtgcgt tgccccggtg
      481 acaggtcggc tgaaggggac aagaaggacc attcaaggct cgtttctttg cgcaacatgt
      541 tcagggcatc gcgggtcgca agagccccct gcttgtactc cgcggggaac tgcggaagat
      601 ccaccgactg gacgccgggc ttcacctcca aactgccggc gccaccgacg aacaacagcc
      661 gcttcacgcc ggcctgcttc acgcccttga tgatcgactc tgtccctttg acctggtggt
      721 tgtagatctc cggatcgctc caccctggat tgaaagcgct gatgaccgcg tcatggcccg
      781 atatcaatcg ggccacctca tcggtgctgt acacatcgcc tttcttcgca tggagcttcg
      841 gatgcgcctg taactcatcc ggatgccgca cgatggccgt cacctcatgg cctcgctcca
      901 aggcttcctt caaaatggcc gacccgacaa atcccgtcgc accaatcaaa gcgatcttca
      961 ttttgacctc ctcgatgaac cggttgtttg aaccgagtgc gacaggattg tgtcgatctc
     1021 ggcgagggtt aatgcttgtc cccattgctt tcccggtgaa gatcgatcag ggtaccggcc
     1081 tcattcagtt ccacggtcac caaagctcct gcctgacgat ttgaacgtga ccgcattgac
     1141 ctgttcctcg atgcctttaa cgatctcccg cagataaggg gggagacagc agataggaac
     1201 caactccttg cgtgtcatgt tgagatccac atctccattc ttcagatcaa catgaatatt
     1261 ctcccagggt acagggccaa tgcggaggcc aataccttca tccgagcgtg ggccaaccct
     1321 acttaggcat tcgttgcaac gcatcgtaat tgcccgccga ctccttcatt cccttctgac
     1381 ggcatcagca ggctccgtac cgaatcaagc agagcagaga tct
//
LOCUS       DASTWE010000413         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_237948, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000413 DASTWE010000000
VERSION     DASTWE010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237948"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1695
                     /locus_tag="VFT30_04405"
     CDS             496..1695
                     /locus_tag="VFT30_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091901.1"
                     /translation="MSEAEDAHRRYLVTGESSYLEIYKKVVKQKPTFTAYLNDLIRDS
                     PEQQQRVGLLDRMMDRQLKAESKAVAQFQEGGFRAVKKMAFEGAGKRELGMIHQIITE
                     MDLHERQAVGRRVVESAAGTRNTIVLLGIGALLQLVLLAWVYYLIRHDITERRRVAGE
                     LQRRGELLEAANKELEAFSYSVSHDLRAPLRHIDGYAALLHKSVEPSLNDKAARYLQT
                     ISDSAKQMGQLIDDLLVFSRMGRQEMLRTVVNLDQLIKSILSDLRLDLQGREISWTIA
                     ALPEVQGDPAMLRQVFMNLIVNAVKFTRTKPMATIEIGVEHPSSTEIVLFVRDNGVGF
                     DMQYAPKLFGVFQRLHRADEFEGTGIGLANVRRIVHRHGGRTWAVGVPDKGATFYVLL
                     PTRRLDS"
     gene            1692..2147
                     /locus_tag="VFT30_04410"
     CDS             1692..2147
                     /locus_tag="VFT30_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091902.1"
                     /translation="MTVAKPILLAEDNPRDAELALAAMEAEHISDKVVLCHDGAEVLD
                     YLYCRGQFKSRLRGNPAVVFLDLKMPKVNGLEVLRTIKADVNLRPIPVVMLTSSREER
                     DLADSYALGANAYVVKPVEFHKFLSAVKELGTFWGIINEPPPESCRSTQ"
     gene            2155..>3197
                     /locus_tag="VFT30_04415"
     CDS             2155..>3197
                     /locus_tag="VFT30_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091903.1"
                     /translation="MTTVLRVLHLETNPQDTDRIKALLTDGGLSCAIRRVETRRTFTS
                     ALNDGQVDLILAEFSMPEFHEGAALEVAQQSAPELPFIFVSGALTETLCIEQMQRGAT
                     DCIPKEGLGRLVPSVCRVVRERQARVARRQAEEALLQSEMQLRQLQKLEAVGRLAGGL
                     AHDFNNLLTVIMGHSQVLLSEMGPDHPLRSRVEEMQKAGDRAKVLIRQLLTFSRKQPS
                     GAKVQSLNATLNNVETMLQRLIGADIQLTLRLSADDLRIKADPALVEQIVMNLVVNAR
                     DAMPTGGKLTVETASIELDHQPKYHGNPISPGEYVRLSVSDTGCGMSSEVQEHIFQPF
                     FTTKEEGKGTGIGL"
BASE COUNT          742 a          823 c          891 g          741 t
ORIGIN      
        1 ggactgggtg gtgagcgcga cgctcatgtt tacgaatttt atcggtcctt cggctactgt
       61 gattcctccg ggtcccccgc taggtggtta gttttctgtt tcgcctctca agagttctgc
      121 tacaattact accacagaga cacgatctca ctgtatggtt tccaaacgat caccgaagtg
      181 tcttgatgca agtcgtcctg atcgatggaa tcggcctcca accatccctc acatgatccc
      241 ctcttaggac ccgagcggcc taaaccagcg gttttcgttc ctgcgggttc atccattttc
      301 tcccgcggtt cagttgcgtg gtttcacagg ctgaccatcg agcagcgtgt cttagccggt
      361 ttcagcctgg tattcgttgg gattctgatt gtcagcgcga tctcctatca caatacgagc
      421 atcctgttga ccaatagtga gctggatggt cgcagtcatg atctcattca actcctgaat
      481 aatatcgatg ctgcgatgag cgaagcagaa gacgcccacc gacgttacct ggtgacggga
      541 gaatcatcgt atctggagat ctacaaaaag gtcgtgaagc agaagccgac attcaccgcg
      601 tatctcaatg acttgatccg cgactcaccg gagcagcagc aacgggtcgg cctcctcgat
      661 cggatgatgg accggcagct gaaagccgaa tcaaaagccg tcgcccagtt tcaagaaggc
      721 ggcttccgag ccgtcaagaa aatggctttc gaaggggccg gaaagcgcga gctcggcatg
      781 attcaccaaa tcattaccga aatggatttg catgagcggc aagcagtcgg acgacgtgta
      841 gtggaatccg ccgccgggac aaggaatacc atcgtactcc tcggcatcgg cgcattgctg
      901 caactggtac tgttggcatg ggtgtactac ctcattcggc atgacatcac ggaacggcga
      961 cgtgtcgccg gggagctaca acgtcgtgga gagcttctgg aggctgcaaa caaggagttg
     1021 gaagcattca gctattcagt ctctcacgac ttacgtgcgc cgttgcgtca tatcgacggc
     1081 tatgccgcgc tcttgcataa atcggtcgag ccttcattga atgataaggc cgctcggtac
     1141 ttgcagacga tttccgactc cgctaaacag atggggcagt tgatcgatga cctgctggtt
     1201 ttctctcgta tgggtcggca ggaaatgctt cgcacggtcg tcaacctgga tcagttgatc
     1261 aagagcatcc tttccgatct tcgcttggac ttgcaaggac gagagatatc atggacaatc
     1321 gctgcactgc ctgaagttca aggcgatccg gccatgctcc gacaggtatt tatgaacttg
     1381 atcgtaaatg cggtgaagtt tacgaggacc aagccgatgg ccacgattga gatcggtgtg
     1441 gagcatccca gctctacgga aattgtcctt tttgtccgcg acaatggcgt gggattcgac
     1501 atgcagtatg cccccaaact gttcggagtg tttcagagat tgcaccgagc cgacgagttc
     1561 gaaggaaccg gtatcggcct cgccaacgtt cgtcggatcg ttcatcgcca cgggggacgg
     1621 acctgggccg taggtgtgcc ggataaggga gcgacgttct atgtactgct cccaacaagg
     1681 aggcttgact catgaccgtg gccaaaccga tcctcctcgc cgaggataac cctcgagacg
     1741 ccgagctggc gttggctgcg atggaggcag agcatatttc cgacaaagtc gttctatgcc
     1801 atgacggagc cgaagtgttg gattacttgt attgtcgtgg gcaattcaag tcacgcctga
     1861 gaggaaatcc ggccgtagtg tttctcgatc tgaaaatgcc aaaagtgaat ggcctcgaag
     1921 tcttgcgtac catcaaggcg gacgtcaatc ttcgtccgat tccagtcgtg atgctgacat
     1981 catcgcgtga ggaacgtgac ctggccgata gctatgcctt gggggccaac gcttacgtcg
     2041 tcaagcccgt ggagtttcac aaatttttgt ccgcggtcaa ggagctggga acgttctggg
     2101 gcatcatcaa tgaacctcct cctgaaagct gtcgttccac ccaatgaccc tgccatgacg
     2161 actgtgttgc gagtgctgca tctcgagact aacccacaag atactgatcg catcaaggcg
     2221 ctgctgaccg atggaggtct ttcttgtgcg atacgacgtg tagaaactcg ccgaactttt
     2281 acttcggctc tgaatgatgg ccaggtggat ctcatcctag ctgaattttc catgcctgag
     2341 tttcatgagg gggccgcctt agaggtagcc caacagtcgg ctcctgagtt accgtttatc
     2401 tttgtctcgg gggcacttac tgagacactg tgcatcgagc aaatgcagcg cggtgcgacg
     2461 gattgcattc ccaaagaagg attgggtcga cttgtcccat cggtgtgccg agtcgtgcgt
     2521 gagcgacaag cgcgagtggc acgaaggcag gcagaggaag cacttctcca aagtgaaatg
     2581 caacttcggc agctacaaaa acttgaagcc gtcggccgtt tggcgggagg actggcccac
     2641 gacttcaata atttgctgac ggtcatcatg ggccatagtc aggttttgct cagtgagatg
     2701 gggccagatc atccgttgag aagtcgggtg gaagaaatgc agaaggcggg tgaccgcgcg
     2761 aaggttctca ttcgtcagtt gctcaccttc agcagaaaac agccttctgg agcgaaggtt
     2821 cagagtctca atgctaccct caataatgta gagaccatgc ttcagaggtt gatcggagcg
     2881 gatattcaac tgacattgag actgagcgcg gacgacctcc gcatcaaagc tgatcctgca
     2941 ttagtcgaac agattgtcat gaatctggtc gtgaatgccc gggatgcgat gccgaccggc
     3001 ggcaagctta cggtcgaaac cgcttcgatc gaactggatc accagccgaa ataccacggc
     3061 aatccgatat cgccaggtga gtacgtgaga ctttcggtat ccgacaccgg ctgcggaatg
     3121 tcctcagaag tacaagagca tattttccag ccatttttta cgaccaagga agaaggaaaa
     3181 ggaacgggaa tcgggct
//
LOCUS       DASTWE010000414         1619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_496244, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000414 DASTWE010000000
VERSION     DASTWE010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496244"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1262)
                     /locus_tag="VFT30_04420"
     CDS             complement(249..1262)
                     /locus_tag="VFT30_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091904.1"
                     /translation="MLGWSRPDPLTRDLIHLINARRHDHGDTDDAIDTVQAFLEQGRE
                     HDLLTVLAALDEEMAEWLFDLVAEASCTLLLDGPNDEKPTYATMAALELPLQANLTAP
                     LKLKIDPIATADLLHKYLQLPAGTVVRVESRLLTDATLEMLNLQLLHDHLVMVADSRR
                     APSVAARLLPPVENTAFLLFELIGAPDPGLPDAWEDRQRREILEGLVKQCPELACAPD
                     TIEAPVYAAYAMFDAQNAVRLHHLADETAAVWRELDPLVRSRTIVHLHTQCGNGVYMP
                     VWTDLFDPELPDEHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVET
                     PDN"
     gene            complement(1395..>1619)
                     /locus_tag="VFT30_04425"
     CDS             complement(1395..>1619)
                     /locus_tag="VFT30_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEU5091905.1"
                     /translation="DDFLERLAPQETVYRHHDTGEDNGDAHLKRQVMGREVVVALTAG
                     KLDFGPWERIFYGEFDGRRRKRVLVKIVGE"
BASE COUNT          377 a          451 c          452 g          339 t
ORIGIN      
        1 acaatcgatg aagccgagtg cgccgagaag agttcgtcct tcgaggggaa atttgcccag
       61 atgatggtcc agactaagat caaccccgcc actgagatgg agtttgtgat cgctttgccg
      121 gaccgcttct cggtgttggc agtcttcatc acggtgagga taaaataaag cggcaggaaa
      181 atcaccgaaa cggaagtttt tcgcgagacc gtgatgtcgg gccgaacaga caggaaggag
      241 tgggtagact agttgtccgg ggtttcgacg atgtgattct cgaacctggt acagccttct
      301 gcgagtaacc gattggtcag catttcacct ggccattcaa tcggcagatc ggcggtgaaa
      361 acccagacat cgggcgaagt ccagactggg ccgtgttcgt ccgggagctc cggatcgaac
      421 aggtcggtcc acacaggcat gtacacccca ttcccgcatt gtgtatggag atgcacgatg
      481 gtgcgggacc tcaccagcgg atccagctcc ctccatacag cggcggtctc atcggcaagg
      541 tgatgaagac gcaccgcatt ctgcgcgtcg aacatagcat acgcagcata cactggcgcc
      601 tcaatcgtgt caggcgcaca ggccagctcc ggacattgct tcaccagccc ttcgagaatc
      661 tcacggcgct gtcgatcctc ccacgcatcg ggcaaacctg gatccggagc gccgatcagc
      721 tcaaacagga ggaacgcggt attttcgacc ggaggaagca accgcgccgc gacagatggg
      781 gcacgcctgg aatcggcgac catcacaaga tggtcatgga gcaattgaag attcaacatc
      841 tccaaggtgg catcggtcaa gagacgcgac tcgacgcgaa caactgttcc agcagggagc
      901 tggagatact tgtgcaggag atcggccgtg gcgatcggat cgatcttcaa tttgagcggg
      961 gcggttaagt tcgcctgcag gggaagctct agagctgcca tcgtggcata agtcggcttt
     1021 tcgtcgttcg gtccgtccaa cagcaatgtg cagctcgcct cggcgaccag atcgaacagc
     1081 cactcggcca tttcttcgtc gagcgcggca agcactgtca gtaagtcgtg ctcccgtcct
     1141 tgctcgagga atgcttgcac ggtatcgatc gcgtcatcgg tgtcgccatg atcgtggcga
     1201 cgggcattga tgagatgaat aagatctcgt gtgagtggat cggggcgaga ccagcctaac
     1261 atcgaggttc cctattgtat gagccatcgg tcacggctcg acaactcaaa aagcatgttg
     1321 ccaaactttt cctacccatg caagcgtcag gactattact cgcgactcgt aaggcatata
     1381 atcgatgcac gtctttactc gccgacaatc ttcaccagaa cccgcttacg gcgacgtccg
     1441 tcgaactcgc cataaaaaat tctttcccaa ggaccgaagt cgagttttcc cgctgtgagg
     1501 gcgacgacaa cttctcgtcc cataacttgt cgtttcagat gagcatcacc attgtcctcg
     1561 cctgtgtcgt gatgccgata caccgtctcc tgaggagcaa gccgttccaa gaaatcgtc
//
LOCUS       DASTWE010000415         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_238021, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000415 DASTWE010000000
VERSION     DASTWE010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238021"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>1197
                     /locus_tag="VFT30_04430"
     CDS             35..>1197
                     /locus_tag="VFT30_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5091906.1"
                     /translation="MISYLGFPVSWPDGRIFGTICVLDRKENSYSELYRKFVLQCRDV
                     LEADLKTVAKLSGELTRSEAYLEEAQRLSHTGSFGWRVSTGEIVWSKETFRIFGYDPS
                     LAVTLDMVIQRVHPDDRAAVQKTLDRASRDGKDFDYEYRLLMPDGSIKHLHVVAHAVK
                     DRTDGLDFIGAIMDVTEARRVEDQMHQARAELAHVARLTTLGEFTAAIAHEINQPLTA
                     IVTSGQACLRWLAHEPPNLEEGRRMVEETVDSAIRAAEIISGLRAMMKKSPAHRELLN
                     INDAISAVIALVAPEVQRNRVVMRAELSNDLPPLLGDRIQLQQVILNLIMNAIEAMRG
                     IDQTQRELIVVSRQDGKRILVEVQDSGAGLEGLELDRLFDAFYTTKPDGMGIGL"
BASE COUNT          281 a          314 c          356 g          246 t
ORIGIN      
        1 ggagtggaat tcgaaccccg atatcaaatt ggggatgatc tcgtatctgg gtttcccggt
       61 cagttggccc gatggccgga tattcggcac gatttgtgtg ctggacagaa aggaaaactc
      121 ctacagcgaa ctgtatcgca aattcgtgtt gcaatgccgc gacgtgctcg aggcggacct
      181 gaagacggtc gcaaaactga gcggagaatt gacgcgaagc gaggcctatc tggaggaagc
      241 gcagaggctc agccatacag ggagcttcgg ctggcgggtt tccaccggcg agatcgtctg
      301 gtcaaaggaa accttcagga tttttggata cgacccgtcg cttgccgtca ctttggatat
      361 ggttattcaa cgcgttcatc cggacgatcg tgctgccgta caaaagactc tcgaccgcgc
      421 atcccgggat ggcaaggatt tcgattatga atatcgattg ctgatgcctg atggttcaat
      481 caagcacctc catgttgtcg ctcacgcggt taaagatcga acggatgggc tcgatttcat
      541 tggcgcgatc atggacgtca cggaggcaag acgagttgaa gaccaaatgc atcaggctcg
      601 ggcggaactc gcgcacgtcg ctcgcttgac gacgctcgga gaatttaccg ccgcgatagc
      661 ccatgaaatc aaccagccgc tcaccgcaat agtgaccagc ggccaggcct gtttgcgctg
      721 gctggcccat gaaccgccca atctcgaaga ggggcggagg atggtcgagg agacggtcga
      781 cagcgccata cgagctgcag aaatcatcag cggtctccgg gccatgatga aaaaatcccc
      841 cgctcacagg gaattgctaa acatcaacga cgcgatttcg gcggtcatcg ctctggttgc
      901 ccccgaggtt cagcgaaacc gagtggtcat gcgggctgag ctatcgaatg atttaccgcc
      961 cctgctggga gatcgaatcc agttgcagca ggtgatcctc aatctaatca tgaatgctat
     1021 cgaagccatg cgagggatcg atcagacgca gcgcgagttg atcgtggttt cgagacaaga
     1081 cggcaaacgt atcctcgtgg aagtccaaga ttcgggcgcg ggactggagg gccttgagct
     1141 tgaccgcctc tttgacgcct tctacacgac caagcccgat ggcatgggaa tcgggct
//
LOCUS       DASTWE010000416         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_496256, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000416 DASTWE010000000
VERSION     DASTWE010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496256"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFT30_04435"
     CDS             complement(<1..228)
                     /locus_tag="VFT30_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091907.1"
                     /translation="MSTATNVPRTILVVDDFDDTRLLLRTWLERRGFRVVEAENGIQA
                     INQAESEAPDLIIMDMQMPELDGLSATRRIRK"
     gene            665..1552
                     /locus_tag="VFT30_04440"
     CDS             665..1552
                     /locus_tag="VFT30_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5091908.1"
                     /translation="MEGLSVGDAFGETFFVNPDLVEGLIAERALAARTWNYTDDTLMA
                     MSVFSVLRQHGHIHQSELARSFAERYDRTRGYGPAMHRLLWEIKCGEDWSERASSLFE
                     GQGSFGNGAAMRVAPVGAFFANDLDAVVDQATRSSVITHTHDEAIAGAIAVAVAVAFA
                     HNLGTSGVTPGRSEFLDSVLPYVPESEVRSRIRQARDMSANASVQFAVSVLGNGVRVS
                     AQDTVPFALWCAGERLSNYEEALWLTVSGLGDRDTTCAIVGGIVASRTGTAGIPAEWV
                     ETRERLPSWPFEERSPDRE"
BASE COUNT          345 a          436 c          478 g          393 t
ORIGIN      
        1 ctttcggatc cgccgcgtgg ctgagagacc gtcgagctcc ggcatttgca tgtccatgat
       61 gatcaggtca ggggcttcag actcggcctg gttgatcgcc tgaattccat tctccgcttc
      121 gaccacacga aaccctctcc gctccagcca cgtgcgcagc agcaggcgtg tatcgtcaaa
      181 atcatcaaca acgagaatcg tgcgtggtac gttggtcgcc gtgctcatgg taacgcctga
      241 gttggggggg aggttggcgc aagcgtagca aaaaagcggc gattggggaa tgaatgttta
      301 gcgcgttggt caggctgtta ctcctacagc gacgcctggt tgcatggcca tccccacgct
      361 accgcgtagg gtactgaccc gactcatctg tgaatctcga cctcggagtc agaaccggac
      421 gcggtagcgt cggtcaggcc gttactccta cagcgacgcc tggttgcatg gccattccca
      481 cgctaccgcg tagggtactg acccgactca cctgcgaatc tcgacctcag agtcagtacc
      541 ggacggggcc ctcgtgcatt tcgccaaaat tgctacggcg caaggaacga ttcttatttg
      601 ttcttcgtag gtctgacaca tggagtcttc actacaacag ccgattcggc gcgcaagatg
      661 taccttggaa gggctatcgg ttggcgatgc attcggcgag actttcttcg tcaatcctga
      721 tctggtcgaa ggattgattg cagaacgtgc tttagcagct cgcacgtgga actacactga
      781 cgacacactg atggcaatgt cagtgttttc agtattacgt caacatgggc atatccatca
      841 atcagagctg gcccggagct tcgccgaacg ttatgaccgc actcgcggct atggtccagc
      901 catgcatcgc ctgttgtggg agatcaaatg tggcgaggat tggtcggaga gggcgagtag
      961 tttgtttgaa ggacaaggtt cattcggaaa tggcgccgcg atgcgtgttg ctcccgtcgg
     1021 tgcattcttt gccaatgacc ttgacgctgt tgtcgaccag gcgactcgat catctgtcat
     1081 cactcacacg catgatgagg ccatagccgg cgcgattgcc gtcgctgttg ccgttgcttt
     1141 cgcacataat ctcggaactt ctggcgtgac gccgggacgg tcagagtttc tggattcggt
     1201 tctaccgtat gttcccgagt ctgaggtacg cagccggatt cgtcaggcga gagatatgtc
     1261 cgccaatgct tctgtccaat ttgctgtttc agttctgggg aatggcgtca gagtatctgc
     1321 tcaagacact gtccccttcg ccttatggtg cgcaggtgaa cgcctgtcga attatgaaga
     1381 ggcgctatgg ctcacagtga gcgggttagg tgatcgcgat acaacgtgcg caattgtggg
     1441 aggcatcgtt gcgtcacgta caggcacagc aggtattccg gcagaatggg tggagacgag
     1501 agaacgcttg cccagttggc catttgagga acgttcacca gatcgcgagt aatgcaacgt
     1561 atcagcgacg gattaccaaa tgattctcgt cgtgcaactc gcggtcgtaa tgcaagacgg
     1621 atcaaatccg ccaactttta ctgtcgcggt ga
//
LOCUS       DASTWE010000417         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_108463, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000417 DASTWE010000000
VERSION     DASTWE010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108463"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..>1780)
                     /locus_tag="VFT30_04445"
     CDS             complement(128..>1780)
                     /locus_tag="VFT30_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEU5091909.1"
                     /translation="SVERIECEPPRSNGNYGCYVHFSDGLVLRVIVRETDIVVGTGPG
                     PITTEHPPPATPLCFYEYSCPEDDLVLTVKKCQSSKPSGSSSPPAVAQRAAASDARGL
                     MTAPPIVHEVLNSSGQPLDAATRAFFESRFGHDFGRVRVHTDERAEQSARNVNAHAYT
                     VGHDIVFSTGKFSPATQEGQRLLAHELTHVVQQTGEKGTRIDSHADGSPGIRLATAHD
                     GFVQRQPAGKTEARYQKLVKQGKWCRDSEKSGELHPGLQCYREIPARQGYPAGNQVCF
                     DKKTGKFVEESPDFVSAVSGQKTDGTCDIPMELTDPPQPFTQRGRRPLGHLVADIATE
                     DPNIIGRQFGRLSGVAMGIALPKGLDSDLVAFAVPTILGFLAGELGERGLPRLNGLAR
                     KHGFLPTISLGAGTNLGLGLSVGLEKRDRPLPLVPINTYLTFALDSTLDLAGEAGGSG
                     TFLAKVGIRIDPRKQGGLFAVGSIGAGLALGDDVSGAASVEVGAGLRATDFLDVQFVR
                     ETVTGDEQEGATYWLMLKLVAPQRVLLGHRKSPVPKKRRGTK"
BASE COUNT          378 a          556 c          476 g          370 t
ORIGIN      
        1 attttgcagc tgtccctctc gaatcatctt tccggcgatc tcccattctc caacaacaac
       61 gcacaagcga tggctcagcg agagcttccg attaatccac tatgcggcgg tacatctgca
      121 tcaagagtca tttcgtccca cgacgtttct tcggcaccgg ggacttccga tgtcctagca
      181 atacacgctg cggagcgacc agcttgagca tcaaccagta ggtcgctcct tcctgctcat
      241 cgccggtaac cgtctctctc acgaattgca catcgagaaa atcagtcgct cggagacctg
      301 caccgacttc tacggacgcc gccccagaca catcgtcacc gagagccaac cccgcgccaa
      361 ttgaaccgac cgcgaaaagt ccaccctgtt tcctcggatc aatacggata cctactttcg
      421 ccaggaacgt cccactgccc cctgcttcac cggccaagtc caaggtcgaa tcgagtgcga
      481 acgtgagata ggtattgata gggacaagtg gcaggggacg atctctcttc tcaagtccca
      541 cactgaggcc aaggccaaga ttggttccgg caccgagact gatggtgggc agaaacccat
      601 gctttcgagc caagccattc aaccgaggta gccctcgttc gccgagttct cccgcgagga
      661 atcccaggat cgtaggaact gcgaacgcga ccaggtccga gtcgaggcct ttcggcaagg
      721 caatgcccat cgccacacca gatagtcgac cgaactgtcg gccgatgatg ttgggatctt
      781 cggtcgcaat atcggcgacc aaatgaccga gtggacggcg accacgttgg gtgaaaggct
      841 gaggtgggtc ggtcagttcc atcggaatat cgcacgtgcc gtccgttttc tgcccggaaa
      901 cagccgagac aaagtccggg ctttcttcga cgaattttcc agtttttttg tcgaaacaca
      961 cctgattacc ggctgggtat ccttggcgcg cgggaatctc acggtaacac tgcagccccg
     1021 gatgaagctc ccccgatttt tctgaatcgc gacaccactt gccttgcttg accagcttct
     1081 ggtaacgggc ttccgtcttg ccggcgggct gacgctgcac gaagccgtca tgtgcggttg
     1141 ccagacgtat ccctggggaa ccatctgcgt gtgaatcaat acgagtcccc ttttcccctg
     1201 tctgctgcac cacatgcgtg agctcgtggg ccagcaatcg ctgaccttct tgcgttgcgg
     1261 gagagaactt cccggtgctg aacacgatgt cgtgacccac cgtataggca tgggcgttca
     1321 catttcgcgc cgactgctca gccctttcat ccgtgtgcac ccgcacccgg ccgaagtcat
     1381 ggccgaaacg agattcaaag aaggcgcgtg tggcggcatc gagaggttgt ccagacgagt
     1441 tcaatacctc gtgtacgatc ggcggtgctg tcatcaaacc ccgtgcgtcc gacgcggcgg
     1501 ctcgttgtgc cacagcgggg ggactcgatg atccgctcgg tttcgagctc tgacacttct
     1561 tcaccgtcag caccagatca tcctcagggc aagagtactc gtaaaagcac agaggcgtgg
     1621 ccggcggcgg atgctcggtg gtgatcggcc ccggccccgt accgacgaca atatccgttt
     1681 cacgcacaat cacgcgcagc acaagaccat cactgaaatg gacgtaacag ccgtagttcc
     1741 cgtttgaccg gggcggttca cactcgatcc gctccacgga
//
LOCUS       DASTWE010000418         1259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_819581, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000418 DASTWE010000000
VERSION     DASTWE010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819581"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..957)
                     /gene="lpxI"
                     /locus_tag="VFT30_04450"
     CDS             complement(115..957)
                     /gene="lpxI"
                     /locus_tag="VFT30_04450"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEU5091910.1"
                     /translation="MPIPDGRIGVIAGNGRFPIIFANNARKMGLQVVAVAHEGETEPE
                     LEQHVDRIHWIKIGQLNKLINAFKSDGVRKVVMLGGIKKTHVYSHARPDFRVLALATK
                     LVLWKDDDILRALAAELEKDGITICESTFGLEGILVEEGTLTSRQPTKKQWVDIRYGW
                     DVAKETGRLDIGQCVVIKDRVVVAVEAVEGTDEAIKRGGELAKDGAVVVKRSKPQQDL
                     RFDLPAVGPRTIEVMRSVKASVLAVEAGRSVMLDRELLISKAEDAGIAVVGLVREEEP
                     SHGT"
     gene            complement(978..>1259)
                     /locus_tag="VFT30_04455"
     CDS             complement(978..>1259)
                     /locus_tag="VFT30_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEU5091911.1"
                     /translation="TFAAGGYRAHLYGLNTVGLQRHSFSADRIALLKKAFDLLFRSGH
                     RTAEAVRLAKKEFKGQTDVAKILAFMEGTKRGISRAVSLDLEREFDQQV"
BASE COUNT          252 a          389 c          319 g          299 t
ORIGIN      
        1 tggccataca ccgttttttt gtctgaattt tgggatctat tgatctgacg atttgttgag
       61 ttccggaaat caatagatca acagatcttc agattccgaa acttcttccc tgactcaggt
      121 cccgtggctc ggctcctctt cgcgaacgag gccgacgacg gcgattccgg cgtcctccgc
      181 cttgctgatc agcagttctc gatccagcat cacggaccga ccggcctcga ctgcgagcac
      241 tgaggccttg acggaacgca tgacctcgat ggtcctggga ccaacggccg gtaggtcgaa
      301 ccggagatcc tgctgcggct tgctccgctt cacgaccacc gcaccatcct tggccaattc
      361 acctccacgc ttgatcgcct catcggttcc ttcgacggct tcgaccgcca cgacgacgcg
      421 atctttaatc acgacacatt ggccaatgtc gaggcgcccc gtctctttgg ccacgtccca
      481 tccatagcgg atatcgaccc actgtttctt ggtgggttgg cgggaagtga gcgtcccctc
      541 ctcaaccaag attccttcca ggccgaacgt cgactcacag atggtgattc catccttttc
      601 gagttctgcg gcaagtgcgc gtaggatatc atcatccttc cagaggacca acttcgtggc
      661 cagcgccagc acgcgaaaat caggacgcgc atggctatag acgtgagttt tcttgatgcc
      721 tcccaacatg accactttcc gcacgccgtc ggacttgaat gcattgatca gcttattgag
      781 ttggccgatc ttgatccagt ggattcgatc gacatgctgt tccagttccg gttcggtttc
      841 gccctcgtgg gccaccgcga ctacctggag ccccatcttg cgggcgttat tcgcaaagat
      901 gatcggaaat cgtccattcc cggcgatcac gccgattcga ccgtctggta taggcatggc
      961 cacgtgcata gggtgattca cacttgttga tcgaactcgc gctccaagtc gaggctgaca
     1021 gcccgcgaga ttccacgttt ggtgccctcc ataaacgcga gaatcttggc cacatcagtc
     1081 tggcccttaa actccttttt ggccagtcga accgcctcag ccgttcggtg gccggatcga
     1141 aagagcaggt caaaggcctt cttcaataat gcaatccgat ccgccgagaa actgtgccgt
     1201 tgtaagccga ccgtgttcag accatacaga tgtgcgcgat acccgccggc tgcgaacgt
//
LOCUS       DASTWE010000419         1335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_625395, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000419 DASTWE010000000
VERSION     DASTWE010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625395"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFT30_04460"
     CDS             complement(<1..368)
                     /locus_tag="VFT30_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091912.1"
                     /translation="MKHSLKNADDLRWLIGHTGGFRSGYVTDVQMSKRRLFDEGSGRE
                     VPAGTTVSVTIRYEIRGMVRVAKLSMTGVTDFSIFEQEGADCSSLGAIQTEFSAGKLR
                     FWFDPQGELYVVCDEAHLDE"
     gene            complement(379..1263)
                     /locus_tag="VFT30_04465"
     CDS             complement(379..1263)
                     /locus_tag="VFT30_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091913.1"
                     /translation="MAVIAPRIERLVDDRPTERPKIPLDVAPRYSLRQLFLSHYHACW
                     YRLGVRLAWSRGPYRERPAEQLQNLSGIQQERMASLRRRFRIRFEQRVEQVTALKQYD
                     YLDILDQAWSAWRLPHSTGGVVQDVGSSNFWYAAVLHAFFRPTEMVGIEVEGHRIYIN
                     GYSRLDYAHGYIQHLPNTEFFVGDYACYNRPADVVTAWYPFVTPDPVLAWRMPLSVLA
                     PGTVFSQVARNLQPHGLFIMVNQGKEEATIAAAWCEKVGLARYGTCTLKPTLRSRIPA
                     VASCWMLSQSLAVSSSLR"
BASE COUNT          318 a          369 c          370 g          278 t
ORIGIN      
        1 atttcatcca gatgtgcctc atcgcatacg acgtagagct ctccctgggg atcaaaccag
       61 aagcgaagtt ttcctgcgct gaattccgtt tgaatggcac caagggagga acaatcggcg
      121 ccttcctgct cgaagatgga aaaatccgtc acgccggtca tgctgagctt ggccactcgc
      181 accatcccgc gaatctcata tcggatggtt actgagacgg tggtaccggc cggcacctcc
      241 cgcccggaac cttcatcgaa gagtcggcgt ttggacatct gtacatcggt cacgtaccct
      301 gagcggaaac cgcctgtatg accgatcaac caccgaagat catccgcgtt cttgagggag
      361 tgtttcacga caccaattct agcgcaggga cgaactcaca gctaggcttt gcgagagcat
      421 ccaacatgat gccaccgcgg gaattcggga gcgcagcgtg ggcttgagcg tacatgtgcc
      481 gtaccgcgcg aggccgactt tctcacacca ggcagctgcg atagtcgcct cctctttgcc
      541 ttggttgacc atgatgaaca ggccgtgggg ttgtaggttt cgggcaacct gcgagaatac
      601 tgtgcccggt gccaatacgg aaagcggcat tcgccaagcc agcacaggat cgggcgtcac
      661 gaaaggatac catgccgtca cgacgtcagc tggacgattg tagcaggcat agtcgccgac
      721 gaaaaactcc gtgttcggca ggtgctgaat ataaccgtgc gcataatcga gacgactata
      781 tccattgatg taaatccgat gcccttccac ctcgataccg accatctcgg tgggccgaaa
      841 aaaggcgtgt aaaacggcgg cgtaccagaa gttacttgaa ccgacgtcct gaacgacacc
      901 gcctgtggaa tgtggtaatc tccaagccga ccaggcttga tcgagtatat cgagatagtc
      961 gtactgcttc agtgcggtga cctgttcaac tcgctgctca aatcgtatgc ggaagcgcct
     1021 tctcaacgac gccatccgct cttgttgaat tccactaaga ttctgaagct gctccgccgg
     1081 ccgttcccgg tagggtcctc gtgaccaagc tagtcgtacc ccaaggcggt accagcacgc
     1141 atgataatga gagagaaata attgtcgcaa tgagtaacgc ggagcgacgt ctagagggat
     1201 cttgggtcgt tctgtgggac ggtcatcaac gaggcgttct atccgtggag ctatgacggc
     1261 catgacgctt actcatcggt cgttcaagca gcgagctaaa ggggcagcaa caaacggaag
     1321 agagattcct caggg
//
LOCUS       DASTWE010000420         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1045877, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000420 DASTWE010000000
VERSION     DASTWE010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045877"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="VFT30_04470"
     CDS             <1..1690
                     /locus_tag="VFT30_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEU5091914.1"
                     /translation="LAAQFGFVPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEI
                     AHDELGSITGTATAVAVLFIVVVALAGLGFAVVNALYHNAWGTFTIAMTIPIGFLMGF
                     YLQRFRPGAIAEVSVLGVVLLLAAVFFGRVVAQSSYAWCFEFDKPMLVWLLAGYGFLA
                     SVLPGWMLLVPRGYLSTFMKLGVVFLLGFGVILMAPTIEMPRVTTFASGGGPIIPGTL
                     FPFLFITIACGAISGFHSLVSSGTTPKMIEQESQAVVGYAAMLLESFVGVMALIAASV
                     LIPGDYLAINTTLSSDALAAMGFAPSRIAELSQMVEVDVAGRPGGAVSLAVGMASIFS
                     ALPGMSGLMAYWYQFALVFEALFILTTIDTGTRVARYLIQEMAGRVYAPCRQMNWLPG
                     VFASSGLVVGAWAYLIGTGNISTIWPMFGAANQLMGMLALCIGTTVLIKMWKSPYLWV
                     TALPMLFVGVITLTGSYEMFWMFLKKAGTLAAGQAFALYLDAVLVALVAVLGLIVLSD
                     SIRQWYGYVVLKKPFTSSEVVVVAGGGTVGRMQTAITADEPGRGFRLPPRTGCC"
     gene            1801..2298
                     /locus_tag="VFT30_04475"
     CDS             1801..2298
                     /locus_tag="VFT30_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU5091915.1"
                     /translation="MADQFSFDVVSEVNMQELKNALDQATKEIKQRFDFKNSKTEITL
                     KEKEKELVVVSDDEYKLKAVQEIIKGKCVKRGVSLKAFSQGAIEPALSGTVRQVAKIQ
                     SGLASDKAKEITKAVKDSKVKVQAQIQGEQVRVLGKSKDELQSAIAFLKDKDFGIDLQ
                     FTNYR"
     gene            2316..>2763
                     /locus_tag="VFT30_04480"
     CDS             2316..>2763
                     /locus_tag="VFT30_04480"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091916.1"
                     /translation="MRNFVAAFVLAAALFGCGRSDPIVVIQLHPKNPDIIYVATNDYI
                     YKTRDGGQTWTNLSKGMSYSRVIAMAIDPVYPATIYAGTKGDAVYKSHDGGQRWVSMR
                     SGLDDATITSVVNQFLFDPADAQHIFLATTMGVFETKNGGEQWTKKM"
BASE COUNT          568 a          625 c          869 g          701 t
ORIGIN      
        1 cttggcggcg cagtttggat ttgtacctgg gtttctctgg ctggtgatcg gggccgtatt
       61 ggccggggcg gtgcaggatt tcatcattct ggtggcttcc atgaggcgca acgggcgctc
      121 gctccctgag attgcgcatg atgaattggg ttccattacc ggcacggcaa cagcagtggc
      181 ggttctcttt attgtcgtcg tcgcgttggc aggtttggga ttcgccgtcg tgaatgccct
      241 ttatcataat gcctggggaa cgtttacgat tgccatgacg attcctatcg gcttcctcat
      301 gggcttctat ctccagaggt ttcgcccagg cgcgatcgcg gaagtgtcgg tgctcggggt
      361 cgttctacta ctggccgcgg tgttcttcgg tcgcgtggtc gcccaatcat cctacgcgtg
      421 gtgcttcgaa ttcgataagc ctatgctcgt ctggctgctg gccggctatg gctttctcgc
      481 ctcagtgttg ccgggatgga tgttgctggt gccgcgtggc tatctctcga ccttcatgaa
      541 gcttggggtc gtatttctcc tcgggttcgg cgtgatcctc atggcaccga cgattgagat
      601 gccgcgcgtg acgaccttcg ccagtggtgg cggaccgatt attcccggca cgctctttcc
      661 ctttctgttc attacgattg cctgcggagc aatctccggc ttccattcac tggtctcatc
      721 aggcacgacg ccaaagatga tcgagcagga atcgcaggcg gtggtgggat acgcggcgat
      781 gctgctggaa agtttcgtcg gtgtcatggc gttgattgcg gcctcggtgc tgattcctgg
      841 cgattacctg gcgattaata cgacgttgag ttcggatgcg ctggcggcca tgggttttgc
      901 accgtctcga attgctgaat tgtcacagat ggtggaagtg gatgtggccg gacgtccggg
      961 aggggctgtg tcactcgcag tcggcatggc gtctattttt tctgcgctgc ctggcatgtc
     1021 gggcttgatg gcctattggt atcaattcgc gctggtgttc gaggcgttgt tcattctcac
     1081 gacgatcgat accggcacgc gcgtggcacg gtatctcatt caagaaatgg cgggaagggt
     1141 ctatgcgcct tgtcgccaga tgaactggtt gccgggggtc tttgcgagta gtggtctggt
     1201 cgtgggggcg tgggcctatt tgatcggtac cggaaacatt tcgacgattt ggccgatgtt
     1261 cggtgccgcg aatcagctga tgggtatgct ggcgctctgc atcgggacga cggtgttgat
     1321 taagatgtgg aagtcgccct atctctgggt gacggcgttg ccgatgcttt tcgtgggtgt
     1381 gattacattg actggatctt atgaaatgtt ctggatgttc ttgaagaaag ccggaacatt
     1441 ggcggcgggg caggcctttg ccctctacct ggacgcggtg ttggtggcac tggtggcagt
     1501 gctgggcttg attgtcttga gcgatagtat caggcagtgg tatggctacg tggtcttgaa
     1561 aaagccattt acgtcaagtg aagtcgtcgt ggtggcgggt ggagggactg tagggcggat
     1621 gcaaactgct attacagctg atgagccggg tagagggttc cgattgccac ctcgtactgg
     1681 atgttgttaa cggatgttga aaaaggtctc cggatgcgtt cccgtactgt taaaaggctc
     1741 aacgtactaa aaggcgtaca tccgaaaagt cgaagaaaaa cggaaagtga gagatgaaat
     1801 atggctgatc agttttcatt cgatgtggtg tcggaagtga atatgcagga gttgaagaat
     1861 gcactggatc aggcgaccaa agaaattaag cagcgatttg attttaaaaa ttcaaagacc
     1921 gagatcacgc tgaaggagaa ggaaaaggag ttggttgtcg tttccgatga tgagtataag
     1981 ctaaaggcag ttcaggagat catcaagggg aaatgtgtga agcgaggggt gtcgctgaaa
     2041 gcctttagcc aaggcgcgat cgaaccggca ttgagcggga ccgtccgaca agtggccaag
     2101 attcagagcg gacttgcctc ggataaggca aaggaaatca ccaaggcggt caaggattca
     2161 aaggtgaaag tgcaagcaca gattcagggc gagcaagtgc gggtgttggg gaagagtaag
     2221 gatgaattgc agtccgcgat cgcattcttg aaggataagg atttcgggat cgacttgcag
     2281 tttacaaatt accgctaagc tgcgtgcaaa ctctcatgcg aaacttcgtt gcggctttcg
     2341 ttcttgcggc cgctctcttc ggttgcggtc gcagtgatcc aatcgtcgtc atccaactcc
     2401 atcccaagaa tcccgatatt atctacgtgg cgacgaacga ctatatttat aagacccgtg
     2461 atggcgggca gacatggacg aatctatcca aaggaatgag ttactcccgt gtgatcgcca
     2521 tggcgatcga tcctgtgtat ccggcgacga tctatgcagg gacgaaaggc gacgccgtct
     2581 ataagagcca tgatggggga cagcgctggg tctcgatgcg ttccgggctc gatgatgcga
     2641 cgataacctc ggtcgtgaac cagtttctct tcgaccctgc cgacgcgcag cacatctttc
     2701 ttgctacgac gatgggtgtg ttcgaaacaa aaaacggcgg cgaacaatgg acgaagaaaa
     2761 tgg
//
LOCUS       DASTWE010000421         1143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1078285, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000421 DASTWE010000000
VERSION     DASTWE010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078285"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFT30_04485"
     CDS             complement(<1..906)
                     /locus_tag="VFT30_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEU5091917.1"
                     /translation="MSERKAFIQACLDRNRTITEICSEFGISRKTGYKILKRYRLFGE
                     FGLEDRSHAVLEHPFRISHEVRERVLALKEKHPDYGPRLIHDWLVQHEPLKHWPAASS
                     IGELFRRQGLVRRRPRRNRDFERAALNSHLTNSKEANLVWTADFKGQFRLNTGWGIYC
                     YPLTVMDLSSHFALGIRALDTISVEPTKQGFIRIFSEYGLPSVIRTDNGVPFAQPNAI
                     GRLGKLAFWWVRLGIKPEHTRPGKPSENGAHERFHRTLKAATAKPASASLRSQQKRFD
                     DFRSEYNDHRPHLSLADRRPPREFYT"
BASE COUNT          227 a          330 c          306 g          280 t
ORIGIN      
        1 cgtgtagaat tcacgaggag gtctccggtc cgccaggctc agatgcggcc ggtgatcgtt
       61 gtactcactc ctgaagtcat cgaagcgctt ctgctgggac cttagtgagg ctgatgccgg
      121 tttcgctgtc gctgctttga gagttcggtg aaatctctcg tgggcaccgt tctcagaagg
      181 ctttccaggc ctcgtgtgct cgggcttgat gccaagtctc acccaccaga acgcaagctt
      241 acccagacgc ccgatcgcat ttggttgcgc aaagggcact ccgttgtcgg ttcgtatcac
      301 actcggtagc ccgtattcgc tgaatatcct gatgaacccc tgcttcgtgg gctcgaccga
      361 tattgtgtcg agtgctctga tccccaaggc gaagtgcgag cttagatcca tgacggtgag
      421 aggatagcaa tagatccccc acccggtgtt gagcctgaac tggcccttga agtcagcggt
      481 ccagaccaaa ttggcctctt tcgagtttgt caggtgagag ttgagcgcgg ctctctcaaa
      541 atcgcggttg cgtcgcggcc ggcgccgcac gagtccctgc cggcggaaga gctctccgat
      601 cgagctcgca gcaggccagt gcttaagagg ctcatgctgc acgagccagt catgaatcag
      661 tcttgggccg taatccgggt gcttctcctt tagagcgagg actcgctccc ggacttcatg
      721 actgatccga aacggatgct cgaggaccgc atgtgagcga tcctcgagtc cgaactctcc
      781 aaagagtctg tagcgcttga gaatcttgta tccagtcttg cgactgatgc cgaactcgct
      841 acagatctcg gtgatcgtcc tattgcgatc gagacaagcc tggataaatg cttttctttc
      901 cgacacgggt gagctctctc tccacggcac ggacacctcc cagatggtgt ccgattgtga
      961 tgccgcaaag cttccgtgtc acccatcaac cggttttaaa ctcacccatc aaccggtctg
     1021 taccctggca cctctcgtct gagaactctc gtctcgtttt gcacaggtcg cctatatttg
     1081 aggattgcat tccaaactgg ttgaatgaac ctcgatcaca tccgcaattt ctgcattgta
     1141 gca
//
LOCUS       DASTWE010000422         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1466016, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000422 DASTWE010000000
VERSION     DASTWE010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466016"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VFT30_04490"
     CDS             complement(<1..1032)
                     /locus_tag="VFT30_04490"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEU5091918.1"
                     /translation="MRTLIVAVSAVFRDLIRVGFRTRTQVIAENLYGDNWRSIRNGRP
                     ADVDPTPGAKLALVVLSRFFPWATALAIVKPCTFVRWHRAGFRLFWRWKSRSVGRPPL
                     PKNIRTLIVTMASENPSWGEGRIANELTLKLGLLVDARTVGKYLKQGRRPRPPRNQRW
                     ATFVQNHASAIIACDFFTSVTATFHVMYVFVAIEIASRRILHINVTDHPTAEWTRQQF
                     REFVDDQSGHRYVIHDRDTVFSAEVDEALKGFGLEILKTPIRSPMANAYCERVIGTIR
                     RECLDFLIPINERHLRRIVREFAIYYNRGRPHSALGPGFPKPNQTTVPANRHRYELPA
                     GYRITKTSIL"
     gene            complement(1045..>1236)
                     /locus_tag="VFT30_04495"
     CDS             complement(1045..>1236)
                     /locus_tag="VFT30_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091919.1"
                     /translation="KCRMPTLVLHENLVHFAAFANHVGYSDIKAPRVPFRAISCAPEI
                     FRAMEGCPCVNVLQIPTWM"
BASE COUNT          291 a          344 c          329 g          272 t
ORIGIN      
        1 aagaatcgaa gtcttagtga ttcggtagcc agcgggcagt tcatatcgat ggcggtttgc
       61 tggaaccgtg gtctgattcg gctttgggaa ccccggtccc aacgcagagt gtggcctgcc
      121 gcgattgtaa tagatcgcaa actctcgaac gatgcgtctg aggtgtcgtt cgttgatcgg
      181 aatcaggaaa tccaagcact ctcgtcgaat cgttccaatt accctttcgc agtacgcatt
      241 cgccatcgga cttcgaatcg gagtcttcaa gatctctaga ccaaatcctt tcaatgcttc
      301 atcgacctca gcagagaaga cagtatcgcg atcgtggatt acataacgat ggcccgactg
      361 atcgtccaca aactcccgaa actgttgccg ggtccactct gcggtcggat gatccgtaac
      421 gtttatatgc aggattcggc gggaggcgat ctcaatggca acaaacacat acatgacatg
      481 aaatgttgct gtgaccgacg tgaagaagtc gcaggcgatg attgcactcg catggttctg
      541 aacaaaagtc gcccatcgct ggttgcgggg cggccgaggt cgtcgtcctt gcttgagata
      601 cttgcccact gttcgagcat ccacaagcaa gcccaacttc agcgttagct cattggcaat
      661 ccgcccttct ccccagctcg gattctcgct cgccatggtg acgatcagcg ttcgtatgtt
      721 cttcggtagc ggcgggcgac ccaccgatcg cgatttccat cgccagaaca gtcggaaacc
      781 ggctcggtgc caacgaacga atgtgcatgg cttcacgatg gcgagtgctg ttgcccaagg
      841 aaagaaccga ctcaacacga ccagtgctag cttcgccccc ggagttgggt cgacgtcggc
      901 gggtcttccg ttcctgatag agcgccagtt gtcgccgtag agattctccg cgatgacctg
      961 cgttcgagtc cgaaatccga cacgaatcag gtcccgaaaa acagccgaca cagcaacgat
     1021 caaggtgcgc aaacagacct cctcctacat ccaagtcggt atctgtaaca cgttaacgca
     1081 agggcatcct tccattgcgc ggaatatttc cggagcacag gaaattgcac ggaacggaac
     1141 ccttggcgct ttgatatccg agtagccgac atgattagcg aaggcggcaa aatgcaccag
     1201 gttctcatgc aggacgagcg tgggcatccg gcattt
//
LOCUS       DASTWE010000423         1463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1530609, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000423 DASTWE010000000
VERSION     DASTWE010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530609"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1463
                     /locus_tag="VFT30_04500"
     CDS             <1..>1463
                     /locus_tag="VFT30_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5091920.1"
                     /translation="PVIGRADEIERVLQILSRRSKNNPVLIGESGVGKTAIVEGLAQR
                     IVQSEVPDNLLSRRVIALDLGSLVAGTKYRGQFEERLKVVMKEIVQAGNIIIFIDELH
                     TLVGAGAAEGSIDASNMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPIHVQ
                     PPNLDETIRIIQGLRDRYEEHHGVEITEDAIVEAVKLSDRYITDRFLPDKAIDLIDET
                     GSRAKLQTYALPSELKAMEQELKKVSREKELSISMQNFEEAVRHREEEERLRKLLDES
                     KREWKKNQEKNKPVIGKEDVAYVVSKMTGIPLFKLEEEESNKLLRMEEFLHKRVVGQN
                     EAISAVARAIRRSRAGLKEARKPIGSFIFLGPTGVGKTELARTLAEFLFNSEDALIRV
                     DMSEYQEKFTSSRLFGAPPGYVWYEEGGQLTEKVRRRPYSVVLFDEIEKAHPDVFNVL
                     LQVLDDGVLTDSLGRKIDFKNTVVIMTSNIGTKMIQK"
BASE COUNT          380 a          339 c          420 g          324 t
ORIGIN      
        1 accccgtgat tggcagggcc gatgaaattg agcgcgttct gcagattctc agtcgaagga
       61 gcaaaaataa tcctgtgctg atcggtgagt ccggtgtcgg gaaaaccgca atcgtcgaag
      121 ggcttgctca acggattgta caatctgaag ttcccgacaa tctgctctct cgtcgggtca
      181 tcgccttgga tctgggatct ctcgtcgctg gtaccaaata ccgcgggcag ttcgaagagc
      241 gtctcaaggt cgtgatgaaa gaaatagtcc aagctggcaa tatcatcatt tttatcgatg
      301 agctgcatac gctggttgga gcaggagccg cagagggatc catcgacgcc tccaatatgt
      361 tgaagccggc cttgtcgcgt ggcgagatcc aatgcattgg ggctacaacg ttggatgagt
      421 accgaaagca tattgaaaaa gatggtgcgt tgaaacgacg attccagccg atccacgttc
      481 agccgcccaa tctcgacgaa accatccgca tcatccaagg gcttcgggac cgttacgaag
      541 agcatcacgg agtggaaatc acggaagacg ccattgttga ggcagtcaag ttgtcagatc
      601 ggtatatcac cgaccggttc ctgccggaca aggcgatcga tttgatcgac gaaaccgggt
      661 cacgggcgaa gctccagacc tacgcgcttc cctctgaatt gaaagcgatg gagcaagaac
      721 tgaagaaggt ttcgcgagag aaggaactct caatatccat gcagaatttc gaggaagccg
      781 tacggcatcg tgaggaggaa gagcggctgc gcaagctgct cgacgaatcc aaacgagaat
      841 ggaaaaagaa tcaggaaaag aacaaacccg tgatcggcaa agaggatgtc gcctacgttg
      901 tctcaaaaat gaccggtatc ccgctcttta agctggagga agaagagtcc aacaagttgt
      961 tgcgcatgga agaatttctc cataaacgag tcgtcggtca gaatgaagcg atctcagcgg
     1021 tcgcccgggc tatccgccga tctcgtgctg gtttgaagga ggctcggaaa ccgatcggct
     1081 cgttcatttt cttggggccg acgggcgtcg gcaagacgga actggccagg acattggctg
     1141 agtttctgtt caatagtgaa gatgcgctga ttcgtgtcga tatgtctgag tatcaggaga
     1201 agtttacgag ttctcgcctc ttcggtgcgc ctcccggcta tgtgtggtat gaagaagggg
     1261 gacagttgac tgaaaaggtc cgccgtcggc cttattccgt cgtattgttc gatgaaatcg
     1321 agaaggcaca tccggatgtg ttcaatgttc tccttcaggt gctggatgat ggggtactga
     1381 ccgatagtct cggacgaaag attgatttca agaacacggt cgtcattatg acgtccaata
     1441 tcgggacgaa aatgattcag aaa
//
LOCUS       DASTWE010000424         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1110717, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000424 DASTWE010000000
VERSION     DASTWE010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110717"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFT30_04505"
     CDS             <1..339
                     /locus_tag="VFT30_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091921.1"
                     /translation="MYWLYGMAVAIGIVVWIGISMVSGRREAFDSELYYRIGMPVVCI
                     ASAIFGFLEPNKSWRWGILPLLGQFSWMLLTQGPGNLLPLGMIFFSILSVPSIITARI
                     GAFLGRKHDT"
     gene            complement(1047..>2144)
                     /locus_tag="VFT30_04510"
     CDS             complement(1047..>2144)
                     /locus_tag="VFT30_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091922.1"
                     /translation="NLLTVINSWSALLLEHASMDEKWKRGLTQIKEAGDKAAGLTRQL
                     LAFTRQQVVEQQVMNLNERVMSIVELMQRVIGEDIHLAVNLDPHLGQVKTDAGQIEQV
                     IMNLVVNARDAMPQGGRLELMTQNVCIEEKDSARQDELHPGSYAVLTVRDSGCGMDVD
                     TMAHIFEPFFTTKERGKGTGLGLSTVYGIIKQSGGNIAVESELNNGTTFTIYLPQFGD
                     RPPDPQSKPVASSLLRGSETVLLVEDDEMVLVLAQVVLEAQHYRVLSARNAEQAIELV
                     RHHAQDIDVVLTDTVMPGMGGPALAERLLQLRPGLRVIMTSGYAGRGQEFIQSLGSQA
                     AFLQKPYTPDMLTKKLREVLDSSGASLPMQG"
BASE COUNT          432 a          610 c          568 g          534 t
ORIGIN      
        1 atgtattggc tatatggaat ggcggtagcg attggaatcg tggtgtggat tggcatctct
       61 atggtttccg gaagaagaga agcctttgat tccgaattgt actatcgcat tggcatgccg
      121 gtcgtctgca tcgcatctgc catattcggt tttctcgagc cgaataagtc gtggcgatgg
      181 gggattctgc ccctactcgg ccagttctcg tggatgctgt taacgcaagg acctggtaat
      241 ttattaccgc ttggcatgat ttttttcagt atcctctctg tgccttcgat cataacggcg
      301 aggatcggag ctttcctggg gagaaagcat gatacatagt ccatatccca attctcgata
      361 cttggctggc cgtcgccttt gaggatcgga tttctcgcca taggtgttcc gtattggcaa
      421 atcctctatg ctaaggtctc actgctagac agcttgtatg acctgagcct gcttgtcatc
      481 tgtatgcttg ccaagaccgc gcgcattcgg caaagcccaa cttcccgaag tcatttttgt
      541 cgtagatgca gctgtaccgg ctgtcatact cgtgtgagtc gccgttgaca cggcgaacga
      601 tccgacctca cataacctgt ttcctatcga ggtcaccatt gcggcagtga tagacgtgct
      661 gtgttcgtca ccagaagcgc tgataggaag tttacttgcc cggttttctc gtcaaagaaa
      721 ctgctaacaa tcctggtcca ttccctcaca catcgtccca ttgagtgctc tacacattct
      781 tcggccacaa tgaattcagc tttggatgtt tcgcgtgata gtcggcgtgg ctgccagagt
      841 caggatctga ggggcagagc ttgaatcgtg ctgcagcaaa agagtgtgcg cggattcgca
      901 gctttttgtt acgcgaacaa cggagacagc accgcatact ggtagggcga tgccgtcgtg
      961 ttcgccgtcc atcaatgccc catgcgccgc taccgcgggt gttatccggt caagcttcga
     1021 cacgtcgtga gaggcttgga ccgccgtcag ccttgcatgg gaagggatgc gccgctcgaa
     1081 tccaaaactt cgcggagctt tttcgtcagc atgtccggcg tatagggctt ctgcaaaaac
     1141 gcggcttgtg atcccaacga ctggatgaat tcctgcccgc gcccggcata gcccgaggtc
     1201 ataatcacac gcagcccagg ccgcaactgc agcagccgct cggccagagc cggtccaccc
     1261 atccccggca tgaccgtatc ggtcaggacc acgtcgatat cctgggcatg atgccggaca
     1321 agttcgatgg cttgctcggc attgcgcgcg gacagtaccc tatagtgctg agcctccaat
     1381 accacctgcg cgagtacgag gaccatctcg tcgtcttcca caagcagtac ggtctccgag
     1441 ccccgcaaga gtgaggaagc gacgggtttg gactgcgggt ccggcggtct gtcgccgaac
     1501 tgtggaagat agatcgtgaa tgtggtgccg ttattcagct cgctctcgac agcaatattc
     1561 ccgccgcttt gcttgataat gccgtacacc gtcgacaatc ccaacccggt ccctttgccc
     1621 cgctcttttg ttgtgaagaa cggctcaaaa atatgcgcca tagtatccac atccatcccg
     1681 cagccgctat cccgcaccgt caggacggca taggaacctg ggtgcagttc atcctgccgg
     1741 gcggagtcct tctcttcgat gcatacattt tgggtcatca actccaatcg gccgccttgc
     1801 ggcatggcat cgcgagcatt gaccaccaga ttcatgatga cctgttcgat ctggccggca
     1861 tcggtcttga cctgcccgag gtgtgggtcc aggttaacgg cgagatggat atcctcgccg
     1921 ataacccgct gcatcaattc gacaatgctc atgacccgtt cattcaggtt catgacctgc
     1981 tgctccacga cttgctggcg cgtgaacgcc aataattgtc tcgtcaagcc agccgccttg
     2041 tctcccgcct ctttgatttg cgtcagtccg cgtttccact tctcgtccat ggacgcatgt
     2101 tcgaggagga gggcgctcca gctattgatg accgtcaaca gatt
//
LOCUS       DASTWE010000425         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_981451, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000425 DASTWE010000000
VERSION     DASTWE010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981451"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFT30_04515"
     CDS             <1..454
                     /locus_tag="VFT30_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091923.1"
                     /translation="TWSITGGRLSGEGRQVTWDLSGVANGSYRATVEVNDGNQHTASG
                     STTVTVADCGDCVPPPPPCPVVSVSCLADVEPNAPITFTASVSGGAPGASWTYNWSVS
                     AGTISSGQGTSTITVDTAGLGGQSVTATVSIGGTDPSCTGRKRSSICN"
     gene            complement(557..>1075)
                     /locus_tag="VFT30_04520"
     CDS             complement(557..>1075)
                     /locus_tag="VFT30_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5091924.1"
                     /translation="MFLSDELILKGKRMFIKKMTAVAMALMFLVISNVMRVEAAPANN
                     LTPEQIEAIVRNMYDKFAAFDAQGTTNCFAADGTIEDSVGTTPIQGTQAILAYQQSFP
                     TLLDELRFQSVDITIGGQEAAVKWRIRLKTKTGNVFFLEGIGIVKVNEDGKIQSARNF
                     FDLAYFQSQLMQ"
BASE COUNT          255 a          280 c          286 g          254 t
ORIGIN      
        1 cacctggtcc ataaccggtg gccgtctcag tggtgaaggt cgccaggtaa cctgggatct
       61 tagtggtgtg gcaaatggca gctatagagc gactgttgaa gtcaatgacg gcaatcagca
      121 caccgccagc ggttcaacga ccgtgacggt ggcggactgc ggggactgtg taccgccgcc
      181 gccgccatgc ccggtcgtct ccgtgagctg cctggctgac gtcgagccga atgcaccgat
      241 tacgtttact gccagcgtct cggggggagc gccaggagcg agctggacct ataactggtc
      301 agtctctgct gggaccatct cgagtggtca aggaacttcg acaatcacag ttgatacagc
      361 cggtctgggt ggtcagagcg taaccgcaac cgtcagcatt ggcggcacgg atccaagctg
      421 caccgggaga aaaagaagta gtatatgcaa ttaaacagaa ccgaagttgc aaagcaaagc
      481 catccacagt tacgccgatt ctgtaatctg tggatggttt gatttgccac gctgttggta
      541 gttcgtagga gcagatttat tgcatcaatt gcgactgaaa atacgcgagg tcaaagaaat
      601 ttctcgccga ctgaattttt ccgtcctcat tgactttcac gataccgatg ccctccagga
      661 agaagacatt tccggtcttg gtcttaagac gtatcctcca tttgacggca gcttcctgac
      721 cgccgatcgt gatgtcaacc gactggaatc tgagctcatc caagagcgtg ggaaacgatt
      781 gctgataagc taggatggct tgtgttccct gtatcggcgt agtcccgacc gagtcttcaa
      841 tcgtgccgtc agcggcaaaa cagtttgtgg tcccctgagc atcgaacgca gcgaatttat
      901 cgtacatatt cctaacaatc gcttcgatct gttccggggt taggttgttg gccggagccg
      961 cctcaacgcg cataacgttc gaaatgacca aaaacataag cgccatcgcc accgccgtca
     1021 tcttctttat aaacattctc tttcctttca atatgagttc atctgagagg aacat
//
LOCUS       DASTWE010000426         1154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_690579, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000426 DASTWE010000000
VERSION     DASTWE010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690579"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..>1154
                     /locus_tag="VFT30_04525"
     CDS             173..>1154
                     /locus_tag="VFT30_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091925.1"
                     /translation="MMKVLRTGKILARWWRIVLVIGVTLCAVLPLVGLGAEVEDTATA
                     LERPGFSLVTRDGSGDAGLYTSVAIGADGLGLISYYDATNRDLKVAHCSNQMCTSATI
                     SSLDRPGDVGRYTSIALGSDDLGLISYYDATNGDLKVAHCSNIACTSATLKTLDHTGN
                     VGQYSSLTIGIDKLPLITYYDVTNGDLKIAHCTDVVCEAATITVLTFLQAEGDVGQYT
                     AVTIAGGQGLISYYDATDHYLKSALCPDLPCTEPTLTTTPDKAGDVGQYSSVTIGMDS
                     RPLISYYDATNGDLKVVHCEEVVCVSPTRTVLESEGNVGQYTSIVIGADGL"
BASE COUNT          257 a          310 c          321 g          266 t
ORIGIN      
        1 tcctcatttc agcgctgcgt ggagtgaact gagcaaccat tggatttcac caatggactc
       61 tcgtttcatc gagatataga ctgtacgagt aaaaggtaat ccgaggggag attcttcgag
      121 actcttgctt gtctctttga ctaagaatca atgagtcatt gggatgaggt gcatgatgaa
      181 ggtgttgaga accggtaaga tcctagcaag gtggtggagg atcgttctgg ttataggagt
      241 gacactttgt gcggtcctgc cgctggtggg tctcggtgcc gaagtagagg atacagctac
      301 agctctggag cgcccaggat tctcgcttgt tactcgagac ggctcgggtg atgccggtct
      361 atatacatcg gtcgctattg gggccgacgg gctaggcctc atcagttact atgatgcgac
      421 gaaccgagat ctgaaagtgg cgcactgttc gaaccagatg tgcacctccg cgacgatcag
      481 ctctctggac cgcccggggg atgtcggccg atacacctcc atcgcgcttg ggagcgacga
      541 cctcggcctc attagttatt acgacgcgac gaacggagat ctgaaagtcg ctcactgctc
      601 gaatattgcc tgtacttcag ccacgctcaa gacgctcgac catactggga acgtcggtca
      661 gtacagttcc ttgaccatcg gcattgacaa gcttcctctc attacctatt acgatgtcac
      721 gaacggcgac ctgaagattg cgcactgcac cgatgtggtc tgtgaggccg cgacgattac
      781 cgtcctgacc tttctgcagg cagaggggga cgtcgggcaa tacaccgctg tcaccattgc
      841 cggagggcag ggtctcatca gctattacga cgcgaccgat cactatctga aatcggccct
      901 ctgtcctgat ttgccttgta cggaacctac tctcacaacg actcccgaca aagccggcga
      961 tgtgggccaa tacagctcgg tgacaatcgg aatggatagt cgccccctca tcagctatta
     1021 cgatgccacg aatggcgacc tcaaagttgt ccactgcgag gaagtcgtgt gcgtctcccc
     1081 aacacgcacc gttcttgaga gcgaaggcaa tgtcggccag tacacgtcca tcgtcattgg
     1141 agccgatggc ctgg
//
LOCUS       DASTWE010000427         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1433879, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000427 DASTWE010000000
VERSION     DASTWE010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433879"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1176)
                     /locus_tag="VFT30_04530"
     CDS             complement(1..1176)
                     /locus_tag="VFT30_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU5091926.1"
                     /translation="MDIRSLLKVMVDREASDLYLTVDASPIYRIHGATQPTDAPPFTN
                     EQLEALALALMRGQQRSEFEEKMEMNLALYYKELGRFRVNIFRQRGNVGLVFRHIKAD
                     IQTVDQLQLPPIIKDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNTIHQGHIITVED
                     PIEFVHQHKKSIITQREVGFDTLNFQNALKNTLRQAPDVILIGEVRDTETMEAAITFA
                     ETGHLCIGTLHSNNANQAIERIMNFFPVERHAQIYLQLSLNLRAIISQRLIPSVDGKR
                     VPALEIMLDTPRIKDLIKKAEVDTLKEAMEQGVDEGCQTFDHVLFQLYKANQISLEQA
                     LINADSANNLRLKIKLEGLKGDEAVNALLDKQMAGGGTDAFKIQGSSSGNVTPIRKR"
     gene            complement(1203..2261)
                     /locus_tag="VFT30_04535"
     CDS             complement(1203..2261)
                     /locus_tag="VFT30_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU5091927.1"
                     /translation="MDISKLLTFSVKEGASDCHISAGEPPMIHIHGDLKKLDHPPLTP
                     DETHALVYDMMNDAQRKTFEEKRECDFSFELGDIARFRVNVFVQQRGLGAVFRNIPTT
                     ILPLEKLGMPPILRQLCDKEKGLILVTGPTGSGKSTTLAAMVDYLNNTFEGHIITIED
                     PIEFVHKSKKCLVNQRELGVHTLSFANALKSALREDPDIVLVGEMRDLETIQLALTAA
                     ETGHLVFGTLHTSSAPKTIDRIIDAFPPAQQAQIRTQLSEALEAVITQTLLKKKTGGR
                     VAALEIMVATTAVRNLIREAKLHQIPGIMQASQKDGMQTMDMALVDLATRGIVHKAEA
                     QSRSMNPNLFGASMTGVA"
BASE COUNT          474 a          601 c          662 g          612 t
ORIGIN      
        1 ttatcgtttg cggatcggag tcacgtttcc ggaagagctc ccttgaattt taaaggcatc
       61 agtgccgccc cctgccatct gcttatcgag caaggcgttc acggcttcat cgcctttgag
      121 cccttcaagt ttgattttta gacggaggtt gttcgcgcta tcagcgttga taagggcttg
      181 ctccagtgag atctggtttg ctttgtataa ctgaaacaac acgtgatcga aggtctggca
      241 gccttcgtcc actccctgtt ccatcgcttc tttcaacgta tcgacttcgg cttttttgat
      301 caggtccttg atgcgcggcg tgtccagcat gatttccaag gccgggacgc gtttgccgtc
      361 gaccgacgga atcagtcgct gtgagataat ggcgcgtaga ttcagtgaca gttgcaggta
      421 gatctgggca tgccgttcga ccgggaaaaa gttcatgatg cgttcgatcg cctggttggc
      481 gttgttggag tgcaaagttc caatgcacag atggccggtt tcggcaaagg taatcgccgc
      541 ttccatggtt tccgtatcgc gcacctcacc gatcagaatc acatccggag cttgacggag
      601 cgtattcttc agcgcgttct ggaagtttaa ggtatcgaac ccgacttcgc gctgcgtgat
      661 gatcgatttc ttgtgctgat gaacgaactc gatcgggtcc tccactgtaa tgatatggcc
      721 ctggtggatg gtattgcgat ggtcgatcat ggccgctaac gacgtcgact tccccgagcc
      781 ggtcgctcct accaccagca cgaggccgcg tttggtcatc gcgatatctt taatgatcgg
      841 aggcagttgc aactgatcga cggtttgaat gtccgctttg atgtgacgaa agaccagtcc
      901 gacattaccc ctttgcctga agatgttgac gcggaaccga cctaattcct tgtagtagag
      961 cgccagattc atctccatct tttcttcgaa ttcactccgt tgttgacctc gcatcaaggc
     1021 taacgccagc gcttcgagct gctcgttggt gaacgggggg gcatcggtcg gctgagtggc
     1081 gccatggatg cggtagatcg gcgaggcgtc cacggtcaaa tacaagtccg acgcttcgcg
     1141 gtccaccatg actttcagga gacttcgaat atccatcaga cgtggtccgt attatgtgcg
     1201 ttctaggcca ccccggtcat cgatgcaccg aaaagattgg ggttcatgct gcgggactgt
     1261 gcttcggcct tatggacgat tccccgcgtc gcgagatcga ccaacgccat gtccattgtt
     1321 tgcatgccgt ctttttggct tgcctgcatg atccccggga tttggtgcag cttggcttcc
     1381 cggatgagat tgcgcacggc cgtcgtcgca accataattt cgagcgcggc gacgcgtccg
     1441 ccggtttttt tcttcagcag cgtttgggtg atgacggctt ccaacgcctc cgatagctgt
     1501 gtcctgattt gcgcctgctg tgccggcggg aatgcatcga tgatgcgatc gatcgtttta
     1561 ggcgcgctcg aggtgtggag ggtaccgaag accaagtgcc cggtttctgc ggcggtcaat
     1621 gccaactgaa tagtctccag gtcccgcatt tcgcccacca ggacgatatc cggatcttcg
     1681 cgtagcgccg acttcagggc gttggcgaaa gatagcgtgt gaacaccgag ctcgcgttga
     1741 ttgaccaggc atttcttgga cttgtggaca aactcgatgg gatcctcgat ggtaatgatg
     1801 tgaccttcaa acgtgttgtt caaatagtcc accatcgcgg caagcgtggt ggacttgccg
     1861 gatccggtcg gtccggtcac caggatcaat cccttttcct tatcgcacag ttgtcggaga
     1921 atcggcggca tgccgagttt ttccaatgga aggatggtgg tcggaatatt ccgaaagaca
     1981 gcgcctaacc ctcgctgttg cacgaacacg ttgacgcgga aacgggctat gtctccgagt
     2041 tcgaacgaaa agtcacattc acgtttctct tcgaaggtct tccgttgagc gtcattcatc
     2101 atgtcgtaaa caagggcgtg tgtttcgtcg ggggtaagag gaggatgatc gagttttttc
     2161 agatcgccgt gaatatgaat catgggaggt tcgccggcac tgatatggca gtcggaggcg
     2221 ccttccttta ctgagaaggt aagcagcttg gaaatatcca tcgcctgtga gcctcagtgt
     2281 tatgggttgc tcgtgaggta aaatctcctg tgcatcatgc catagcaaag cttgaaagca
     2341 agaaaatag
//
LOCUS       DASTWE010000428         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_916688, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000428 DASTWE010000000
VERSION     DASTWE010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916688"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFT30_04540"
     CDS             complement(<1..343)
                     /locus_tag="VFT30_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5091928.1"
                     /translation="MEPLIPEAIAKHIQSVLTKIRSAEEKAGRPAGTVRLVAATKTVT
                     VEHIAEGVRAGLSILGENRVQEALPKIAAFTQAPVHWHFIGQLQRRKVRFVIGLFDLI
                     HSVDSLELAQEI"
     gene            complement(428..1171)
                     /gene="pgeF"
                     /locus_tag="VFT30_04545"
     CDS             complement(428..1171)
                     /gene="pgeF"
                     /locus_tag="VFT30_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEU5091929.1"
                     /translation="MTSASVITVPAFADAGSQVRHFFGTRQHAMELGLEVGIPRRGIA
                     GVASSSWTLSVKQVHGTEALVVDRALAPTDRFVGGWDALVTDQPGIMVAVRTADCVPI
                     LIHDPKRRVVAAVHAGWRGAVAGIVPKTLALLEARFGSCLEHVRISIGPSAGVCCYEV
                     DEPVLDRLCRGFPGWNKVVRTGGDGKAHLDLKTLVKEQARALGATPKSITTVNLCTIC
                     HEDLFYSYRREKKVNGTMISAIGLPMRRG"
     gene            complement(1213..2409)
                     /gene="ftsZ"
                     /locus_tag="VFT30_04550"
     CDS             complement(1213..2409)
                     /gene="ftsZ"
                     /locus_tag="VFT30_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5091930.1"
                     /translation="MFSFQDDILSPVRIKVIGIGGAGCNAINTMITSGLARVDFIASN
                     TDLQALDRSQAPYKIQLGPERTRGLGAGAKPEIGRDAALESKEHIRECLEGADMVFVT
                     AGMGGGTGTGAAPIVASIAREMGILTVGVVTKPFQYEGQRRHKHAEEGIRDLRRHVDT
                     LLIIPNQRLLGIVDKSTPLLEAFKVADDVLRQAIQGIADVITTTGHVNVDFADVRTVM
                     SHTGRAVMGMGVSYGPNRAIEAAQKAMCSPLLEEGSVEGARGVLLNITGGPSMSLHEI
                     EEAASIIQQTADPEANIIVGQVINPDMGEELIITVIATGFEREEDSAATTIGAERGMG
                     RPAKPVPSVLAGMGASLGVDRPMKDLDRPAFLRRMNDMRESMDRAVLTAEDEWDVPTF
                     LRKQTD"
     gene            complement(2471..>3172)
                     /gene="ftsA"
                     /locus_tag="VFT30_04555"
     CDS             complement(2471..>3172)
                     /gene="ftsA"
                     /locus_tag="VFT30_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5091931.1"
                     /translation="GLDVVDIILQPLASSEAVLSQEERDLGVAMVDLGGGTTDLAIFL
                     DGSIRHSAVLPIGGQNLTKDLAIGLLTSQTEAEKIKTQYGVARTELVTGHQIVDVPSV
                     GDRPARTFSRRDIAEILEPRVDEMFELVRREIVRAGYEGMLGAGVVITGGTSLLDGML
                     DAAEKVLNLPARRGIPSGVGGLRDIVGHPSHSTGVGLLLHARRHVDELETAGLRNGGT
                     WAKVRGWTKRVLEVF"
BASE COUNT          663 a         1010 c          804 g          695 t
ORIGIN      
        1 caatttcttg cgccagctct agactatcta ccgaatggat caagtcgaat agaccgatca
       61 cgaaccgtac cttccttcgt tgcagctgtc cgataaaatg ccaatgaacc ggtgcttggg
      121 tgaaggcggc tattttagga agcgcttcct gtacacgatt ttcacccaag atggacaagc
      181 cggcacgcac accttccgca atatgctcga cggtcacggt cttcgtcgcg gccaccagtc
      241 gcacggtacc ggcgggacgc ccggcttttt cttccgccga ccgaattttc gtgagaaccg
      301 actgaatatg tttcgctatc gcttctggga tgagcggttc catcgtcctc tcgcacgagt
      361 cgcggccgct cattcagcga accgtcctta ttctagcgga agagtcccag aattggcaga
      421 gtctgactta tcctcgcctc atcggcaacc cgatggcact gatcatggtg ccgttgactt
      481 tcttctcccg tcgataggaa taaaagaggt cttcgtggca aatcgtacag aggttgacgg
      541 tcgtgatgga cttcggagtc gccccgaggg cccgtgcctg ttctttgacg agcgttttca
      601 aatcaagatg cgcttttccg tctcctcccg ttcgtaccac tttgttccaa cccggaaatc
      661 cccgacacag acggtcgaga accggctcat ccacttcata gcagcaaacc ccggcggatg
      721 ggccgatact gatccgtaca tgctccaaac acgagccaaa gcgagcctcc aacagtgcca
      781 gcgttttagg gacaatgccg gcaacagctc cccgccagcc ggcatggacg gcggccacga
      841 cccgacgttt agggtcatgt atcaggattg gaacacaatc tgctgtccgc accgccacca
      901 tgattccagg ttgatcggtg accaaggcat cccagccgcc cacaaatcgg tccgtcgggg
      961 ccaaagcacg atccaccacc agcgcctccg ttccatggac ttgcttcact gagagtgtcc
     1021 atgaggacga ggctacgcct gcgatccccc tccgcggaat gcccacttca aggccaagct
     1081 ccatggcatg ctgacgggtt ccgaaaaaat ggcgaacctg actccccgca tccgcgaacg
     1141 ccggcaccgt aatgaccgat gcgcttgtca tactcaagtt caatacctca tcacgtaaag
     1201 agatgcacga gatcagtctg tttgtttgcg aagaaatgtc ggcacatccc attcatcttc
     1261 ggcagtcaag accgcccggt ccatcgactc tcgcatatcg ttcattcttc tcaaaaaggc
     1321 ggggcgatcg aggtctttca tcggtcgatc tacccccagg gacgcgccca tgcctgccaa
     1381 tactgatggg acgggtttgg ccggtcgccc cattcccctc tcagctccaa tcgtggtggc
     1441 tgctgagtcc tcttctcgtt caaatccagt cgcgattacc gtaatgataa gttcctcgcc
     1501 catgtctggg ttgatgacct gcccgacaat gatattggct tcgggatctg ccgtctgttg
     1561 gatgattgag gcggcttctt cgatctcatg cagcgacatg ctggggcctc ccgtaatgtt
     1621 caggagaacg ccgcgggctc cctcgacact cccttcctcg aggagaggac tgcacatggc
     1681 cttttgagcc gcttcgatcg cccgattcgg cccataagaa acgcccatgc ccatgaccgc
     1741 tcgtccggtg tgggacatca cggtacggac atcggcaaaa tcgacgttca catgtccggt
     1801 ggtcgtaatg acgtcggcga tgccttggat agcttgccgc aatacatcat ccgcgacttt
     1861 gaacgcttcg agaagcgggg tagatttatc gacgatcccc agcagccgct ggttcggaat
     1921 gatgagcaat gtatcgacat gccgacgcaa atcgcggatt ccttcttccg cgtgcttgtg
     1981 tcgtcgttgg ccttcatact gaaacggttt cgtgacgacg cctaccgtga ggatgcccat
     2041 ttcacgggcg atgctggcca caatgggagc ggctccggtg ccggttcctc ctcccatccc
     2101 ggccgtgaca aagaccatgt cggcaccttc gagacattct cggatgtgct ctttactctc
     2161 aagcgcagca tctctaccga tctccggctt tgcccccgcc cccaacccac gggttcgttc
     2221 cggcccgagt tggatcttat aaggggcctg cgatcgatca agcgcctgaa gatccgtgtt
     2281 actcgcgata aaatcgacac gcgcgagtcc ggaggtgatc attgtgttga tcgcgttgca
     2341 cccggcaccg ccgattccga tcaccttgat gcggaccggc gacagtatat catcctgaaa
     2401 tgagaacatt ggggcacctc ctcatcgcct cgcacgacga cccacctgcc gcccgcaagg
     2461 gttttaaaga ttaaaagacc tccaataccc gcttcgtcca cccacgcact ttggcccagg
     2521 ttcccccatt gcgaagccct gccgtttcca gctcatcgac atgtcgccga gcgtgtaaca
     2581 atagacccac ccccgttgaa tgactggggt ggccgacaat atcgcgcaac cctccaacac
     2641 cggacggtat gcctcggcgt gccggcaagt tcaagacttt ctccgcggca tcgagcatgc
     2701 cgtccaataa ggacgtgccg ccggtaatca cgaccccggc acccagcatc ccttcgtagc
     2761 cggcgcgtac aatttccctg cggacgagct caaacatctc atcaacacgc ggctccaaaa
     2821 tctcggcgat atcccgtctg gaaaatgttc gagccggccg atctcccacc gacggcacgt
     2881 cgacgatctg atgcccggtc accaactcag tccgtgcaac accatactgg gtcttgatct
     2941 tctcagcctc ggtctgtgag gtaaggagac cgatggctaa atccttcgtc aagttttgtc
     3001 cgccgatggg aaggaccgct gagtgacgaa tgctgccgtc cagaaaaata gccaggtccg
     3061 tcgttcctcc tcccaagtcc accattgcta cgcctaggtc acgttcctct tggctcaaca
     3121 ccgcctcgct ggacgccaac ggctggagaa tgatgtcgac cacgtcgagt cc
//
LOCUS       DASTWE010000429         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1369159, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000429 DASTWE010000000
VERSION     DASTWE010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369159"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFT30_04560"
     CDS             complement(<1..733)
                     /locus_tag="VFT30_04560"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5091932.1"
                     /translation="MLDLDRHATVIEQRAANPSRVMIALPNAWLSQPAQTKITDGTIP
                     SPFIVTQLPSHAVVVSLPVTSFRTYKHFTLSHPPRVVIDVTPPIAQVPPSALDLPEVL
                     QRSTSPASQPMTPRAKGYATIVIDPGHGGKDPGARGIRKTEEKDITLKVGLQLRELLS
                     KQAGVRVLMTRDRDVFIELEDRAKFANNNEADFFVSIHVNSHPSRAVKGIEIYHFGEA
                     KDQRALAVAARENGTPISNTGVGWEY"
     gene            1103..1624
                     /locus_tag="VFT30_04565"
     CDS             1103..1624
                     /locus_tag="VFT30_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU5091933.1"
                     /translation="MPTHLIVDGYNLLAGAGSFFGHLESAREKLVRDLAAYRHRKHHL
                     ITVVFDGWQQGQLSEQREHRAGVQVIYSKRGERADQVIQRLAREYGADCAVVSSDHEI
                     VNSARAHGAFVVGSREFVQKLRALSPAAGALPNKELDVEDENRPRRGPEKKGNPRKLP
                     KAQRQRDRQLRRF"
     gene            complement(1611..2381)
                     /locus_tag="VFT30_04570"
     CDS             complement(1611..2381)
                     /locus_tag="VFT30_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5091934.1"
                     /translation="MLIDTHTHLDDARYNDDREAVITRAREAGVEAFVTIGCDLATSQ
                     AAVTLADRFPFVYASIGVHPHEVKHVQDGWYDEFRRLANNKNVVAYGEIGLDYHYNHS
                     SPKEQRDRFREQIRVARELTLPIIIHTREAQEDTVTILKEERASEIGGVFHCFSGDSW
                     LAKEALDLGFYLSFSGILTFQNAAPLREIAKNTPLDRVLIETDCPYLTPIPYRGKRNE
                     PVYVSQVAKQLATLHPELSLDEVQRTTTVNAKRLFKIA"
BASE COUNT          599 a          698 c          816 g          589 t
ORIGIN      
        1 ggtattccca tccgactccg gtgttgctga tcggggtgcc gttctcccgc gccgcgaccg
       61 ccagagcccg ctgatctttg gcctctccga agtgataaat ttcaatccct ttaaccgcgc
      121 gcgacggatg ggagttgaca tggatagaga caaagaagtc ggcttcgttg ttgttcgcga
      181 acttggctcg atcttccagc tcaatgaaca catcacggtc ccgagtcatc aacacgcgca
      241 caccggcctg cttgctcaga agctcacgaa gctgtagtcc caccttgagg gtaatgtcct
      301 tttcctcggt ctttcggatt ccccgtgcgc cagggtcttt tccgccatgg ccgggatcga
      361 tcacaatggt cgcgtatccc ttggcgcgcg gcgtcatggg ctgtgaggcg ggagaggttg
      421 accgctgaag gacctctggc aagtcaagtg cggaaggagg gacttgtgcg atgggtggag
      481 tcacgtcaat gaccacgcga ggcggatgag aaagcgtgaa atgcttatag gttcggaacg
      541 atgtgacagg gagagataca acgacagcgt gagaggggag ttgagtgacg ataaagggcg
      601 atgggatggt cccatccgtg atcttggttt gcgctggttg gctgagccat gcgttgggaa
      661 gggcgatcat cacgcgactt ggattggcgg ctcgctgctc gatgacagtg gcatgacgat
      721 cgagatccaa caccagtctg atcctctccg agctcgtcat cactcgaaaa ttgcgaacgg
      781 taacgggggc aagggaagca gtcgttcctc tgacgcggct gtgttccgat gacatcaccg
      841 gcagaagacg gtgatcatcg ggggaccaag cccaagccgc ttggatcagg atcccgtatg
      901 cgtacaggag gaagatggca ctggacaaga ggagagcgag gggcctgatt gaaagcagcc
      961 gaggctttcg tacttgcatg agctttttag cagaagacaa tgggaaacta ttctcagatg
     1021 tccgccgtct tgtcaagaat tacgccgatt ggctatcgtt attcgcattg actgtctgct
     1081 tacgaccacg atagcgtgaa ggatgccgac gcatctgatc gtcgatgggt ataacctgct
     1141 ggctggtgcc ggaagctttt tcgggcatct tgaatcggct cgcgaaaaat tggtgcgaga
     1201 tctagcagct tatcggcatc gaaagcatca cctcatcacc gtcgtgttcg acggctggca
     1261 gcagggccag ctatcggaac aacgggagca tcgtgccgga gtccaggtaa tctattcgaa
     1321 acggggtgag cgggcggatc aggtcatcca acggttggca cgagagtatg gagcagactg
     1381 tgcggtcgtc agttccgatc acgagattgt gaattccgct agggcccatg gggcctttgt
     1441 cgtgggatct cgcgagtttg tgcagaagct ccgggcgctg tccccggcgg ccggtgcctt
     1501 accgaacaaa gaactcgacg ttgaggacga gaatcgtcca aggcgtggac cggagaagaa
     1561 gggaaatcct cgcaagctcc caaaggcgca aaggcaacga gaccgccaac tcaggcgatt
     1621 ttgaaaagcc gtttggcgtt cacagtggtt gttcgttgga cctcgtccag ggagagttcg
     1681 ggatggagcg tggcaagctg cttagccacc tgggatacat agaccggttc gttgcgcttt
     1741 cctcggtagg gaatcggagt gagatacggg cagtcggttt cgatcagcac gcgatcgagg
     1801 ggggtatttt tagcgatttc tctcaacggc gccgcattct gaaatgtcag gattccagaa
     1861 aacgacaggt aaaatccaag atccagcgct tctttagcca gccagctatc tccggaaaaa
     1921 cagtgaaaca ccccaccaat ctctgacgct ctctcttcct tcagaattgt aaccgtatct
     1981 tcctgtgctt ccctcgtgtg aatgattatg ggcagcgtca gttcccgcgc cacccgaatc
     2041 tgctcacgga atcggtcgcg ctgttctttc ggcgaagagt gattgtaatg gtagtccagc
     2101 ccgatttccc cgtaggccac gacgttcttg ttgttcgcca ggcgccggaa ttcatcgtac
     2161 cagccgtcct ggacatgttt gacctcgtgc ggatggacgc cgatggaggc atacacgaac
     2221 ggaaatcgat cggcgagcgt gacggctgct tgactggtcg ccagatcgca gccgatggtg
     2281 acgaatgcct caacgccggc ttcccgcgcg cgggtgatga cagcctccct atcgtcgtta
     2341 tagcgagcat cgtcgaggtg cgtgtgcgtg tcgatcaaca tcggacacat cgtagcatgg
     2401 gcgattgctc aagaaatagt cgaacagcgc gctgcatggc tgaaggccac cgaaacaatc
     2461 ggtcgtggaa ctgccttcgg ctgcctctaa catgctccaa gtagaagcgg cttcgcgaca
     2521 gaagcgtttt tgaagccagg gagagggatt gacagaacga aatgccatga tgtactgata
     2581 ccaattatca caatcaatca atgccgaggc ccacgatgac ataggccatc ggtcagaggc
     2641 cagagtactt tacgtccaga gcccaagatc gccaggcggc ggtcttgggc ttttttgtgc
     2701 cg
//
LOCUS       DASTWE010000430         1856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_625690, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000430 DASTWE010000000
VERSION     DASTWE010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625690"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFT30_04575"
     CDS             complement(<1..743)
                     /locus_tag="VFT30_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091935.1"
                     /translation="MLLTWDPVDKRYYSHGLDRGVLYLPGEDPIAWNGLQNFEENSQG
                     TSTLYYRDGVVYLADAEAGDFSGKITAMFYPDEFGVCIGIPEVTDGLYADAQKPKRFG
                     LSYRTLIGSGTQGDMFGYQIHLVYNCMATIGQRSRKSMGASPEPVPFTFDVNCTPVKL
                     TGFRPSAHFAIDSRNMSQATIDDLEDLIYGAGSTPGVLPDPDVLFDMMNFGNAITVVS
                     YADGTFDVTGSADNVFMTSMDGFQINNINA"
     gene            complement(752..1261)
                     /locus_tag="VFT30_04580"
     CDS             complement(752..1261)
                     /locus_tag="VFT30_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091936.1"
                     /translation="MLELKVHDGEREVVLRFEHSLLSLSKWESKYKKPFMVVGQKSAR
                     EMLDYYECMLLPPEDDPDLLVLLDPEQMDQLTTYINEPQTASSVPDEGRKGGDGETIT
                     SELIYYWLSQMRIPFQPTETWHLSRIVMLVQITGWKQQPAKKRKPAEVYREWIAENER
                     RKKLFKTRG"
     gene            complement(1292..>1856)
                     /locus_tag="VFT30_04585"
     CDS             complement(1292..>1856)
                     /locus_tag="VFT30_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091937.1"
                     /translation="GVAWSGLTAVTESPAGAESNKQYADNIVYVNLLSAEEFNGTIEA
                     FTFPPEFLGHDGVAKTANGLQIGMQGRPTFGFSWQNLKGNAEDEDLGFVLNMAYGLQA
                     SPSEKANNTVNDSPELKQFSWSVSSTPVVVAGFKPTAITRVDSTDPEVDPAGLEALLD
                     ALYGRGAAPTPRLPLPDEADDILAGTP"
BASE COUNT          396 a          526 c          521 g          413 t
ORIGIN      
        1 gcattgatgt tgttgatctg gaatccatcc atgctcgtca tgaagacgtt gtcagcggac
       61 ccggtcacgt cgaacgtgcc atccgcatag ctgacaaccg tgatcgcgtt gccgaagttc
      121 atcatgtcaa atagaacatc cggatctggc aggacaccag gtgttgaacc ggcgccgtaa
      181 atcagatcct ccagatcgtc gattgttgcc tgtgacatgt tgcgactgtc aatggcaaag
      241 tgcgcggaag gacggaaacc cgtcaacttg accggcgtgc agttgacatc gaacgtgaac
      301 ggtactggct ctggtgaagc acccatgctt ttacgactac gctgaccaat ggtggccatg
      361 cagttgtaaa cgaggtgtat ctgatagccg aacatgtcgc cttgtgtgcc actcccgatt
      421 agtgttcggt aagacagacc aaaacgcttc ggcttctgag catcagcata gagaccgtcg
      481 gtgacttccg gaattccgat gcacacaccg aattcatcgg gatagaacat ggcagtgatc
      541 ttcccggaga agtcgcctgc ctcagcgtct gctaggtaga caactccgtc acggtagtac
      601 aacgtggacg tgccctgcga attctcttca aagttctgca gaccattcca tgcgatggga
      661 tcttctcccg gaaggtacaa cacgccccgg tcgaggccgt gagagtagta acgcttatca
      721 actgggtccc acgtgagtag cacttggcct cctaaccacg ggtcttgaag agcttcttac
      781 gacgttcgtt ctctgcaatc cattcgcggt atacctcagc aggcttccgc ttcttcgccg
      841 gctgctgctt ccaaccggtg atctgtacaa gcatgacaat tcggctcaag tgccaagtct
      901 cagtgggctg aaacggaatc cgcatctgtg aaagccagta atagatgagc tcgctggtga
      961 tcgtctcacc atctccgccc ttccttccct catcaggaac ggacgaagcg gtttgaggct
     1021 cattgatgta ggtcgtcaat tgatccatct gctctggatc gagaagaact aacaagtcag
     1081 gatcatcttc cggaggaaga agcatgcact cgtagtaatc aagcatctct cgagcagact
     1141 tttgcccaac gaccatgaat ggtttcttgt attttgactc ccattttgac agagagagaa
     1201 gagagtgctc aaaccgaagc acgacttcac gttctccgtc gtgaacttta agttcgagca
     1261 ctctcttctc ctactctttg tcggtgacga actacggagt gccggccagg atgtcgtctg
     1321 cctcgtcggg cagcggcaac ctcggcgtcg gggccgcacc acgaccgtag agtgcatcca
     1381 gcagagcctc gaggcccgcc gggtccactt ccgggtcggt ggagtccacc ctggtgatgg
     1441 cagtcggctt gaagcccgcg acgacgaccg gggtcgacga gacggaccag ctgaactgct
     1501 tgagctccgg agagtcgttg acggtgttgt tggccttctc cgacggagac gcctgcaggc
     1561 cgtaggccat gttgagcacg aagcccaggt cctcgtcctc ggcgttcccc ttcaggttct
     1621 gccaggagaa accgaaggtc ggacgaccct gcatcccgat ctggagcccg ttggccgtct
     1681 tggcgacgcc gtcgtggccc aggaactcgg gcgggaaggt gaaggcctcg atggtgccgt
     1741 tgaactcctc cgcggagagg aggttgacgt agacgatgtt gtccgcgtac tgcttgttgg
     1801 actcggcacc cgccggcgac tcggtgacgg ccgtgagacc gctccaagcg acacct
//
LOCUS       DASTWE010000431         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_76636, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000431 DASTWE010000000
VERSION     DASTWE010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76636"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VFT30_04590"
     CDS             complement(<1..1368)
                     /locus_tag="VFT30_04590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5091938.1"
                     /translation="MMHSFPWLTALIFLPLIGAAAVFFVKDTSARFIALAVTVADLLI
                     SLPLWWLFDASSSHMQFAESAVWISSPTINYRLGVDGISLPLVLMTTVLMPLCVLISW
                     HSIESRVQSFMAMLLIMEGAMIGVFLALDFVLFYVFWEAMLIPMYLLIGVWGGPNRLY
                     AAIKFFLYTLAGSVLLLVAILVLYFQGGHTFDILQLSQGTYSKSMQFWVFLAFFAAFA
                     VKVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGTYGFLRFSLPMLPDASQAFTPLM
                     VVLSIIAIIYGAYMALAQADLKKLIAYSSVSHMGFVTLGLFMFNIQGIEGAVMQMVNH
                     GITTGGLFLCVGIIYERTHSRQIADNIGLTKPMPRYATFLVIFALSSLGLPGTNSFVG
                     EFMVLAGTFLWSKIATAFASLGIILAAAYLLWMVQRVAFGVPAPHMLPKLRDVNLREM
                     VTLVPL"
     gene            complement(1482..>2876)
                     /locus_tag="VFT30_04595"
     CDS             complement(1482..>2876)
                     /locus_tag="VFT30_04595"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5091939.1"
                     /translation="VNRVGDFGFMLGMLLVWYQFGSLNYLDIFPALHEATDLTMNLLS
                     PFGGTLEVSIFTLIGLLLFTGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAG
                     VFMVARLAPIYNLSPTAMSVVAITGAATMVLGATIALTQTDIKRVVAYSTVSQLGYMI
                     MACGLGAYASGMYHLLTHGAFKALLFLGCGSVIIALHHEQDMSHMGGLKDKLPITYWT
                     FVVGSLALAGFPLTAGFFSKDDILVAAWSAGGLGQVLTFFGLLTALLTAFYSFRLVFV
                     TFWGSSRVDPHHAAHVHEPSQTMTTPLLILALFSILTGYLGIPSFLEPVFSSGRDTAA
                     HHGSEGLMIMAAATAMGLIGIAAAYYMYVLNPDLPERLARQWGSLYRGSLNKWYVDEA
                     YDRLFVTPTHITASELWKRVDVNLIDGAVNGIARGVAWGGWLLRLIQSGQTQHYALAM
                     AAGVVVIATMLLMF"
BASE COUNT          764 a          784 c          815 g          513 t
ORIGIN      
        1 gagcggcacc agggtaacca tttcgcgcag gttgacatcg cgtagttttg gaagcatgtg
       61 tggggccggc acaccgaaag cgacacgttg aaccatccag agcaagtagg cggcggcgag
      121 gatgattccc aatgatgcga aggctgtagc gatcttgctc cagagaaagg tccctgccag
      181 aaccataaac tccccgacaa aactgttcgt gccgggcaat cccaacgaag acaaggcaaa
      241 aatgaccagg aatgtcgcat accgtggcat tggtttggtc agcccgatat tgtccgcgat
      301 ttgtcggctg tgggtacgtt cgtaaattat cccgacgcag aggaagagcc caccggtggt
      361 gatgccgtgg ttcaccatct gcatcacggc gccttcgatg ccttgaatgt taaacatgaa
      421 cagaccgagc gtgacgaacc ccatgtggct gacgctcgaa taagcgatga gctttttcag
      481 gtcggcttgg gccaacgcca tgtatgcgcc atagatgata gcgataatcg aaaggaccac
      541 catcaacggc gtaaacgctt gagatgcgtc cggcaacatc ggcaaactga agcgcaaaaa
      601 tccataggtg cccatcttga gcaggacgct ggcgagaatc acgctgcctg ccgtcggtgc
      661 ttccacgtgc gcgtcgggca gccatgtatg gaaggggaac atgggcacct tgacggcgaa
      721 tgcggcgaag aaggctagga agacccagaa ttgcatggac ttggaataag tgccttggct
      781 caattgaaga atgtcgaatg tatggccgcc ctggaaatag agcaccaaaa tcgcgaccag
      841 cagcaagacg ctgccggcta gggtatagag aaagaattta atcgcggcat aaagccgatt
      901 cggcccaccc cagactccga tcaagagata catcgggatc agcatcgctt cccaaaacac
      961 atagaacagt acaaaatcca gcgccaagaa cacgccgatc atggcgcctt ccatgatcag
     1021 cagcatggcc atgaagctct ggactctcga ttcaatggaa tgccaagaaa tcaggacgca
     1081 gagcggcatc aggaccgtcg tcatgaggac cagcggcaaa ctgatgccat cgacacccag
     1141 ccgatagttg atcgtcggcg acgagatcca cacggccgat tccgcgaact gcatgtggct
     1201 ggatgatgca tcaaacagcc accagagggg gagtgagatc aataagtcag cgacggtgac
     1261 agcgagggcg ataaagcgag ccgaggtgtc cttcacgaaa aagaccgcag ctgctccgat
     1321 cagcgggaga aagatcagtg ccgtaagcca aggaaatgaa tgcatcattg gtcaatcgtc
     1381 attggtcaat ggttacgcaa caagaattaa tcccgacgga aggaagcgtg gcaatatctc
     1441 gctcgtcaat gaccgctgac cgatgaccat tgaccctttc actagaacat caacaacatc
     1501 gttgcaatga caacaacccc cgctgccatc gccagcgcgt aatgttgagt ctgtccgctc
     1561 tggatcagtc tcaacaacca tccaccccac gcgacaccac gcgcgatgcc gttgaccgct
     1621 ccgtcgatga gattgacgtc gactcgcttc catagctctg aagcggttat gtgcgttggc
     1681 gtgacaaaca ggcggtcata cgcttcatcc acgtaccatt tgttcaacga gccgcgatag
     1741 aggctacccc actgtcgggc caagcgctcc ggaagatccg ggttgagtac atacatataa
     1801 taggcggccg caatgccgat cagacccatt gctgtcgctg cggccataat catgagcccc
     1861 tccgagccat gatgggcagc cgtgtcacga ccacttgaaa acaccggctc aaggaaggag
     1921 gggatgccga gatagcccgt cagaatgctg aacagcgcaa gaatcaggag cggcgtcgtc
     1981 atcgtctgtg acggctcatg gacatgagct gcgtggtgcg gatcgacccg cgaagagccc
     2041 caaaaggtca cgaataccag gcggaagctg tagaaggctg tcagcagcgc cgttagtaga
     2101 ccgaaaaatg tcaggacttg accaaggccg cccgccgacc aagcggccac gaggatatcg
     2161 tctttactga agaatccagc ggtgagtggg aatccggcga gcgctagcga gccgacgaca
     2221 aatgtccagt aggtgatcgg caacttgtct ttgaggccac ccatgtggct catgtcctgc
     2281 tcatggtgca gagcaataat gaccgaacca cagcccagga agagaagggc tttgaacgcg
     2341 ccatgtgtca ataagtgata catgccggag gcataggctc cgaggccgca agccatgatc
     2401 atataaccga gctgactgac cgtcgaatac gcgacgacac gtttgatgtc ggtttgcgtc
     2461 aacgcaatag tggcgccgag gaccatcgtc gccgcgcctg taatggcgac cacgctcatc
     2521 gcggttggcg aaaggttata gatcggggcg agacgcgcca ccatgaaaac gccggcagtg
     2581 accatcgtgg cggcgtgaat gagggccgaa atcggcgtag gcccttccat cgcatcaggc
     2641 aaccagacgt gaagcggcac ttgtgccgat ttacccaccg cgccggtgaa caggaggagt
     2701 ccgatcagcg tgaatatcga aacctccaac gttccaccga aagggctgag aaggttcatc
     2761 gtcaaatcag tcgcctcatg gagggcagga aagatgtcga ggtagttcag cgaaccgaat
     2821 tggtaccaga cgagcagcat gcccagcatg aacccgaaat cgcccacgcg attcac
//
LOCUS       DASTWE010000432         1294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_561681, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000432 DASTWE010000000
VERSION     DASTWE010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561681"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1294)
                     /locus_tag="VFT30_04600"
     CDS             complement(<1..>1294)
                     /locus_tag="VFT30_04600"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU5091940.1"
                     /translation="RKFLEEQIEDAKKQGVLFSLHMKATMMKISDPKIFGHGVTVYYK
                     DVFEKYADTFKKLGVDPDNGLGDVYAKIKSLPDDQRKAIEADIQAVYQKRPPMAMVNS
                     DKGITNLHVPSDIIIDASMPPVIRDSGKMWNPEGKLQDVKCVIPDSSYAPVYHEVVEF
                     CKKNGAFDPRTMGTIPNVGLMAQAAEEYGSHDKTFKAPGNGTIRIVDAAGNTLHEHKV
                     EEGDIWRACQVKDAPIQDWVKLAVTRARATGAPAVFWLNKDRAHDAELIKKVNTYLSK
                     HDTTGLEIKIMAPADACRFSIERMKDGKETISCTGNVLRDYLTDLFPILEIGTSAKML
                     SIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLAKVGNNA
                     VAKILADTLDQANAKFLESNKSPARKVGEIDNRGSHFYL"
BASE COUNT          261 a          388 c          327 g          318 t
ORIGIN      
        1 aggtagaaat ggctgccgcg gttgtcgatc tcgccaacct tgcgggcggg cgatttattg
       61 ctctccaaga acttcgcatt ggcctgatcc agggtgtccg ccagaatctt ggctaccgca
      121 ttatttccca ctttcgccag gtgctccagc gaagcagcca acgccagaaa ctcaccgagc
      181 gaatcccaac gcaagtagcc ttcttcctgg aactgctgta cgtgcttagg agccgatcct
      241 cccgcgccgg tctcgaacaa cccgccgccg ttcagcaacg gaacgattga gagcattttg
      301 gcactggtgc cgatttcgag gattgggaac agatccgtga gatagtcacg cagcacgttg
      361 cccgtgcagg aaatcgtttc cttgccgtcc ttcatccgtt cgatcgagaa acggcaagca
      421 tcagctggtg ccatgatctt gatctcaagg ccggttgtat cgtgcttcga cagatacgta
      481 ttgaccttct taatcagctc agcgtcatgt gcgcgatcct tattcaacca gaaaactgcc
      541 ggcgcaccgg tcgccctggc acgggtgacc gctaatttca cccagtcctg aatcggggca
      601 tccttcacct ggcaagctcg ccaaatgtca ccttcttcga ccttgtgctc gtgcaacgtg
      661 ttgccggccg catccacgat gcggatcgtg ccgttgcccg gcgccttgaa ggtcttatcg
      721 tgcgagccgt actcttccgc cgcttgcgcc atcaggccga cgttcggaat cgtccccata
      781 gtacgcggat cgaaagcccc gtttttctta cagaactcga cgacttcatg atagaccggc
      841 gcataacttg agtcgggaat cacgcacttc acgtcctgca gtttaccttc cggattccac
      901 atcttgccac tgtcgcggat caccggaggc atggaggcgt cgatgatgat gtcgctcggc
      961 acgtggaggt tggtgatgcc cttgtcactg ttcaccatcg ccatcggcgg ccgcttctga
     1021 tacaccgcct gaatgtccgc ttcgattgcc ttgcgttgat catctggtaa ggacttaatc
     1081 ttggcataca cgtctccgag accgttatcc ggatccactc cgagtttctt gaaggtgtcg
     1141 gcatacttct cgaacacgtc tttatagtag acagtgacgc catgaccgaa gatcttcggg
     1201 tccgaaatct tcatcatggt ggccttcata tggagagaaa acagcacacc ctgtttcttg
     1261 gcatcttcga tctgctcttc caagaacttg cgca
//
LOCUS       DASTWE010000433         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_852104, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000433 DASTWE010000000
VERSION     DASTWE010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852104"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /gene="nadA"
                     /locus_tag="VFT30_04605"
     CDS             complement(<1..963)
                     /gene="nadA"
                     /locus_tag="VFT30_04605"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU5091941.1"
                     /translation="MNSLTGGRNIVTATATVSRPITEYQTQSAEELYRRTAAAKSALG
                     ERVMILGHNYQRDEVIQHADFRGDSLLLAKLAAERSERPYIVFCGVHFMAETADILSH
                     SQQTVILPDMAAGCSMADMAAIEQVDQCWEALGRVVPVEDTVMPAVYVNSAAVLKAFC
                     GEHGGITCTSSNAKAVIEWCWARREKILFFPDEHLGRNTANKMGIPREQMIVWDPYQP
                     NGGNTRDAIKRAKLILWKGHCSVHQMFQPAHVDHFRKQYPDGKVIVHPECHEDVVNKA
                     DLIGSTEFIIRTVTAAPAGTAWAIGTELNLVNRLKHELTDKKVFF"
BASE COUNT          259 a          328 c          295 g          266 t
ORIGIN      
        1 gaagaacact ttcttgtcgg tgagttcgtg tttcagtcga ttgacgagat tcaattccgt
       61 cccaatcgcc cacgccgtgc cggccggcgc agcggtcacc gtgcgaatga tgaattcggt
      121 cgatccgatg agatccgcct tattgaccac gtcttcgtga cattctggat gcacaatgac
      181 tttgccgtcc ggatactgtt tgcggaagtg atcgacatgt gccggttgaa acatttgatg
      241 gacgctgcaa tgacctttcc ataagatcag tttggctcgt ttgatggcat ccctggtatt
      301 tccgccgttg ggttgatagg ggtcccacac gatcatctgt tcgcggggaa tgcccatctt
      361 gttcgccgta ttgcggccca agtgctcatc aggaaagaag agaatttttt cacgcctggc
      421 ccagcaccat tcaattacgg ctttggcatt ggaggacgta caggtgattc ctccatgctc
      481 gccacaaaac gccttgagca ccgccgcgga gtttacatag acagccggca tcacggtatc
      541 ttccacgggc accacacgcc ccaatgcctc ccagcactgg tcaacctgtt cgattgccgc
      601 catatcggcc atcgagcaac ccgcagccat gtccggcaaa atcactgttt gttgagaatg
      661 gctgaggatg tccgccgtct cagccataaa gtgcacgcca caaaagacga tgtaggggcg
      721 ttcggagcgt tcggcggcca gcttggcaag caaaagcgaa tcaccgcgga aatcagcatg
      781 ctgaatcact tcatcccgct ggtagttatg gcccaggatc atcactcgct caccgagtgc
      841 gctcttcgcc gccgccgtcc gacggtataa ttcctccgcc gactgcgttt ggtactcagt
      901 gattggcctc gatactgtcg cagtagccgt cacaatattc ctcccacccg taagggaatt
      961 cattctacga tagccttccc tcacaatcaa cgaatggccc tacgaggaaa agtcctagtc
     1021 agtcgcccca aaggatagta gacggatggg tctaatagcc gattgacttg ctcatctcca
     1081 aggaatacga tcagcaccaa tagctagagc taggggggcc ttgaggctga gagtccgaac
     1141 gatcggac
//
LOCUS       DASTWE010000434         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1014072, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000434 DASTWE010000000
VERSION     DASTWE010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014072"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..>1071)
                     /locus_tag="VFT30_04610"
     CDS             complement(125..>1071)
                     /locus_tag="VFT30_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5091942.1"
                     /translation="NEPSVVAVEKKTERVMAVGADAKRMLGRTPGNILAVRPMKEGVI
                     ADFEKAEAMLSHFIQKAHNRTAFVRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYL
                     IEEPVAAAIGAGLPITEPSGNMVVDIGGGTTDIAVISLGGIVYSESVKVAGDRMDDAI
                     MNYIKKKYNLLIGEHMAERIKFEIGSAYPFEERKTMMIKGRDLISGIPRTLVVDDAEV
                     REALQEPIGTIVNAIKVALENTPPELAGDIIDRGIVLTGGGSLLKGMDTRFREETNLP
                     IITVDDPLTSVVLGVGKILDELDLLRKVSVMSQCSTSR"
BASE COUNT          225 a          331 c          284 g          231 t
ORIGIN      
        1 gcaacccctg gctttgacta ggaaggagaa agagggcggc gagcaaaagg gcgagcagca
       61 ctatggcgag acgcctggca ccgtatgttg agcgcgatat agccatccac actcgggatg
      121 gagatcatcg agaggtactg cactgcgaca tgactgaaac cttgcgaagg aggtccaact
      181 cgtccagaat cttcccgacg cccagaacca ccgaagtcag cgggtcgtca accgtaatga
      241 tcggaagatt ggtttcttca cgaaaccgag tgtccattcc cttcagcaag gatccgccgc
      301 cagtcagcac gataccgcgg tcgatgatgt ctccggccaa ttcaggcggt gtgttttcaa
      361 gcgccacctt gatggcatta acgatggttc caatgggttc ctgcaacgct tctctgacct
      421 cggcatcatc caccacgaga gtccgtggga tgccggagat caaatcgcgt cccttgatca
      481 tcatggtctt ccgctcttcg aagggatagg ccgacccgat ctcgaactta atgcgctcgg
      541 ccatatgctc gccaatgagc agattatact tcttcttaat gtagttcatg atagcgtcgt
      601 ccatccggtc accggccacc ttgaccgact cgctatacac gatgcccccc aaggagatca
      661 ccgcgatatc ggtggtgccg ccgccgatgt ccaccaccat gtttcccgat ggttcggtaa
      721 tcggcagtcc tgcgccgatg gccgccgcaa ccggctcttc gatcaaatac acttcgcgcg
      781 caccggccag ttccgcagaa tcccggacag ctcgctgttc cacctgcgta atcctggatg
      841 gtacgccgat gatgatgcgc ggtcgcacaa aagccgtgcg gttgtgcgcc ttctgaatga
      901 agtggctcaa catcgcctcg gctttttcaa agtcggcgat caccccttcc ttcatcggac
      961 gcaccgcgag aatgttcccc ggggtacgtc ccagcatgcg cttcgcgtcg gccccgaccg
     1021 ccatgacacg ttccgtcttt ttttctacgg cgaccacgga gggttcgttg a
//
LOCUS       DASTWE010000435         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_432235, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000435 DASTWE010000000
VERSION     DASTWE010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432235"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFT30_04615"
     CDS             complement(<1..1241)
                     /locus_tag="VFT30_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5091943.1"
                     /translation="MSKKDVASAERVLLDATAQLPYSTQLQFGIAALYKKTGQDSKAR
                     ERYIALVQEYKDKPVGLEARVKLAEMDFQAGKQTEAERQVQEVLKDNPRSSDGLTLLG
                     RMALVRRNGKDAVQAFRTVLHDQPESATVHYLLGQAYQLTGETNLAKESFERAVALYP
                     DQVDAKRSLAVLESKSGRYQQARARLDDLLKQRPDDIAALDMLMTLDLVMKNWVGAEQ
                     TLGRIRQVAVENHVALMAEGRLYEAQRRLNDAMNAYERAITLAPNEPEPLLSLVKLEV
                     AQGRMVRSQARLETILAARPDHPFAHGLLGEVLSVSGAYEAAVHHYREAARLNPKWIT
                     PWLNWATSSLSKKKPDVAAQVLQEGLKANPDSEELHMLLASALFEQRQIDLAMAAYET
                     TLRLNPRNLLAANNLAVLLVD"
BASE COUNT          280 a          376 c          313 g          292 t
ORIGIN      
        1 tgatcaacga gtaagacggc cagattattc gccgccagca agttgcgagg attcagccgc
       61 aaggttgtct catacgcagc catagcgaga tcgatttgcc tttgctcgaa gagcgcagag
      121 gccaagagca tgtgcaactc ctcactatcg gggttggcct tgagcccctc ttggagaacc
      181 tgtgcggcca catccggttt tttcttggac aacgacgatg tggcccagtt gagccaaggc
      241 gtgatccact tgggattcag tctcgcagct tcacgatagt gatgcactgc tgcttcatac
      301 gctccggaga cggacaggac ttctcccaac aatccgtgag cgaacggatg atcaggacga
      361 gccgcgagaa tcgtttccaa ccgggcctgt gaccggacca tccgaccttg agccacctct
      421 agcttcacga gggacaggag aggctcgggt tcgttcggag ccaaagttat tgcacgctca
      481 tacgcattca tcgcgtcatt caagcgacgt tgcgcctcat acaatcgccc ttccgccatc
      541 aaggccacat gattctcgac cgctacctgg cggatccgcc ccaacgtctg ttccgctccg
      601 acccagtttt tcatcaccaa gtccagcgtc atcagcatgt ccagcgcagc aatatcgtcg
      661 ggacgttgtt tcaacagatc atccagccga gcacgcgcct gctggtatcg gccgctttta
      721 ctttccaata ccgcgaggga ccgtttagca tcgacctggt caggatacag agccacggcc
      781 cgctcaaaac tctctttcgc caggttggtc tctccggtaa gttgataagc ctggccaaga
      841 agataatgca ccgtcgctga ttcaggctga tcgtgtaaaa ccgtacgaaa ggcttgaact
      901 gcgtctttcc cattccgtct gactagtgcc atgcgcccta aaagggtcag accgtccgaa
      961 gagcgggggt tgtcttttaa cacttcctgc acttgacgct cagcttcggt ttgcttgcca
     1021 gcctggaagt ccatctccgc caacttcacc ctggcttcca acccaaccgg cttatcctta
     1081 tattcttgaa ccaaggcgat ataacgctca cgtgcttttg agtcttgccc ggtcttcttg
     1141 taaagcgcgg caatcccgaa ttgaagttgg gttgaatacg gcaactgtgc cgtggcatcc
     1201 agcaatacac gttcggcaga cgcaacatcc ttcttgctca taaaaaagtc ggccaaggca
     1261 a
//
LOCUS       DASTWE010000436         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1175385, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000436 DASTWE010000000
VERSION     DASTWE010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175385"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1357
                     /locus_tag="VFT30_04620"
     CDS             <1..>1357
                     /locus_tag="VFT30_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091944.1"
                     /translation="WIQTSIDALQEFSVQQNAYSAEHSRSGSFFNAITKSGTNRVHGT
                     LYEFLRNEKLDARNFFAGARAQLKRNQFGASAGGPVILPKIYNGTNKTFVFFNYEGMR
                     ERQGNIISRNSPTPAMLQGDFSALRNIIYDPLSTAPSPTGTGVVRTPFPGNKIPADRL
                     SPQAQFFNQFLTTAATPGGQFVFSPSTAVDTDQVTTRLDHTINDNHRVFFRWSLNNNR
                     LSEPGGSPLLGVADSKTRGQNYTVSINSNLRANLVNEFRFNTLYGLISLNPFLLGRDF
                     NKEAGIVGLEETRRSFDTGSFPDFSWSGYTGLTGSAFDQRPKTQDRYTREFVDNLTWI
                     KGRQVFKFGAKIRYYSWLGTDSKEYMGLWNFNGQNTENPASTSGTGDSFADWMLGLPS
                     SANRAYPSDTFGGDYTAWHFFVQDDIKLTSKLTLNVGLRYEYTPFATAYRGQTGTFDG
                     TQ"
BASE COUNT          325 a          384 c          363 g          285 t
ORIGIN      
        1 tggatccaga cgtccatcga tgcgctccag gagttcagcg ttcaacagaa cgcatattcg
       61 gccgagcaca gccggtcggg gagcttcttc aacgcgatca caaaatcagg gacgaatcgt
      121 gtacacggga cgctttacga attccttcgg aacgaaaagc tggatgcgcg caatttcttt
      181 gcgggcgcca gggctcaact gaagcgaaat cagttcggcg cgtccgcagg tggtcccgtg
      241 atcctcccga aaatctacaa tggcacgaac aagacgttcg tcttcttcaa ttacgaagga
      301 atgcgagagc ggcaaggcaa catcatcagc cgcaacagtc cgactccagc tatgctgcag
      361 ggcgacttca gcgcacttcg gaatatcatt tacgatccgc tctcgactgc gccttccccg
      421 acgggaacgg gcgtggtgag gacaccattt ccgggcaaca agattccagc cgatcgattg
      481 tcgccccaag ctcaattctt caaccaattt ctcacgactg cagcaacacc tggcggtcag
      541 ttcgtgtttt cgccgtcaac ggcggtggac acggatcagg tcacgacgcg attggaccac
      601 accatcaatg acaaccaccg tgtatttttc cgctggagtt tgaacaataa ccggttgagc
      661 gagcccggtg ggtctccttt gctgggcgtc gcggactcca agacgcgagg acaaaactat
      721 acggtcagca ttaacagcaa tcttcgtgca aatctcgtga acgaatttcg gttcaacacg
      781 ctttacgggc tgatcagtct caatccattt ctcctgggac gtgacttcaa caaggaagcc
      841 ggcatcgtcg gcctcgagga aacccgacga tcctttgaca cgggttcctt ccccgacttc
      901 agttggtccg gctatacggg tctgaccggt tcggcattcg atcaacgtcc gaagactcag
      961 gaccggtaca ctcgcgagtt cgtcgacaac ctgacgtgga ttaaagggag gcaggtattc
     1021 aagttcggag cgaagattcg ctattactcc tggttaggca ccgatagcaa ggagtatatg
     1081 gggctttgga acttcaatgg tcaaaatacc gagaaccctg cgagcacgag cggcacaggc
     1141 gattcgttcg ccgactggat gttgggcctg ccgtcgagcg caaaccgggc atatccgtct
     1201 gacacgttcg ggggagacta cacggcctgg cacttcttcg tacaagacga cataaaactc
     1261 acctcgaagc tcaccttgaa tgttggacta cgctacgagt acacgccgtt tgcaaccgct
     1321 tatcgcgggc agaccggcac gtttgacgga acgcaag
//
LOCUS       DASTWE010000437         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_658605, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000437 DASTWE010000000
VERSION     DASTWE010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658605"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFT30_04625"
     CDS             complement(<1..515)
                     /locus_tag="VFT30_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEU5091945.1"
                     /translation="MKAADAMTGLTQLYDAQRIVLDAAPVLGLEKISILDALGRVLSE
                     DIIAERDNPPWDNSAMDGFAVRWEDIKQAQTIQKPVTLSIIEDVPAGKMPSKTVGSGQ
                     AIRIMTGAPIPRGADTVLKVEDTEPTPDSVRVFKAEPKGANIRPQGEDVKKGDYIIAK
                     GTRIRPSEAGML"
     gene            complement(512..>1219)
                     /locus_tag="VFT30_04630"
     CDS             complement(512..>1219)
                     /locus_tag="VFT30_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5091946.1"
                     /translation="DMDIHGPNIPKMVGAEGQKLKISTSGGIIPFQAYNLKIASMSLL
                     LQNSDDPIIWRDAYKYEFINQLLGGVDWQDLNFLLIDLPPGTGNESVTTIDLLGTVSG
                     AVIVTTPQEVALLDSRKSVTFCKDSEVPIIGIVENMSGLECPHCHTHIDVFRKGGGEA
                     SALDMGVPFLGRIPLDPDVVTQSDAGEPFALFNSDTPTAEAYHGIANQVEAFCKKGAS
                     LLKMGRGSAGPLKGVSQ"
BASE COUNT          258 a          368 c          308 g          285 t
ORIGIN      
        1 agcattcccg cttcacttgg acggattcgc gtccctttgg caatgatgta atcacccttc
       61 ttcacgtctt caccctgcgg cctaatgttg gctcctttgg gttctgcctt aaacacccgg
      121 accgaatcag gcgtaggctc cgtatcttca accttcagaa ctgtatccgc ccctcgaggg
      181 atcggggctc ccgtcatgat ccggatggcc tgaccagaac cgactgtttt ggacggcatt
      241 ttaccggcag gaacatcctc aatgatcgag agagtgaccg gcttctgaat agtctgcgcc
      301 tgtttgatat cctcccatct gacggcaaac ccatccatag ctgagttatc ccacggcgga
      361 ttgtcacgct cagcgatgat gtcttcgctc aacacacggc ccaaggcgtc cagaatcgag
      421 atcttttcaa gacccagcac cggcgccgca tcgagtacga tcctttgcgc atcgtagagt
      481 tgggtcaatc cggtcatggc atccgcagct ttcactggct gactcccttc agcggtcccg
      541 cgctcccccg ccccattttc aacaacgatg cgcctttttt acagaaggct tcaacctgat
      601 tggcgatacc gtggtacgcc tccgccgtcg gcgtgtctga gttgaacaac gcaaagggtt
      661 cacccgcgtc ggactgtgtg accacgtccg ggtcgagcgg aatccgccct aaaaagggaa
      721 ctcccatgtc gagcgcggac gcttccccgc cgcccttccg gaatacatca atatgagtat
      781 gacaatgggg gcattcgagc ccgctcatat tttctacgat cccgatgatc ggcacctcgc
      841 tatccttgca aaacgtgacc gattttcggg aatcaaggag cgccacctct tggggagtcg
      901 tgacgatgac ggcaccgctc accgtgccga gcagatcgat cgtcgtgacc gactcgttcc
      961 cggttcccgg cgggagatcg atcaggagaa agttcaaatc ctgccaatcg acacccccga
     1021 gtagttgatt gatgaattca tacttgtagg catcgcgcca gataatggga tcatcggaat
     1081 tctgcagcag gagcgacatc gatgcgatct taagattata cgcctgaaag ggaatgatcc
     1141 cccccgacgt gctgatcttg agtttctgcc cttcggcacc gaccattttg ggaatgttgg
     1201 gaccgtgaat gtccatatc
//
LOCUS       DASTWE010000438         1004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_625887, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000438 DASTWE010000000
VERSION     DASTWE010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625887"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            610..>1004
                     /locus_tag="VFT30_04635"
     CDS             610..>1004
                     /locus_tag="VFT30_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5091947.1"
                     /translation="MIVRKKKITLPEGLIGVISDTHGLTRPEALAALKGVELIIHGGD
                     IGKVEVLKSLRTIAPVCAIRGNNDRDAWAKNLPDVLHLQVNGAKIHVIHNVQEFDGDP
                     NASGIRAVISGHSHKPSIVTRDGVLFVNPG"
BASE COUNT          235 a          267 c          265 g          237 t
ORIGIN      
        1 aagcgttggt tgcgatgcta aggagcccgc taagacatcg atctaacaaa tgccaacgaa
       61 ttggcttggt caccgggggc tgctaggagt ggagtggttg cgataccatg cgccgcacgg
      121 ctgcaattag agccgatggc ctgacaggtt tctgtagaaa ttgtgcgtgc ggcccaagta
      181 tcgtgtctcc gccggactgt gacatatacg cggaaagcat aatcaccggc actttgggcc
      241 atcttgcttg aatgttggca ataagttcta atccgttaac atttggcatc accatgtcgg
      301 ttatgactac attaattgcg ggatagtgtg tctcaagcaa ttcaattgct tcggctccgt
      361 ccattgcctc taagacttca tagccttgcg accgcaattt ttgagcaaaa tcataccgcg
      421 gtatcggctc gtcttcgact aagagaatca cacggcgatc atagcattgg ttgggaatag
      481 cacaagggct attttaacaa tgtgctgtgc taagtcatta aagcggcgtt cccgcccgaa
      541 catctgacct attagcagtg cattccgagt atgctcttca acccggctcg tcagctaaat
      601 gagtctcgca tgatagttcg caagaaaaaa atcacgctcc ctgagggact catcggcgtc
      661 atttccgata cccacggcct gactcggcct gaagcgctgg cggcgctcaa aggcgtcgaa
      721 ctgatcattc acggtggcga tatcgggaaa gtcgaagtcc tcaagtcgtt acgcacgatt
      781 gcgcccgtgt gcgcgatccg cggcaacaac gaccgtgacg cctgggcgaa gaacctgccg
      841 gatgtgctcc atctccaagt taacggtgcc aagattcatg tgatccataa cgtccaggaa
      901 ttcgatggcg accccaatgc ctctgggatt cgtgccgtca tcagtggtca ctcgcacaag
      961 ccgagcatcg tgacgcgcga cggcgtgctg ttcgtcaatc ccgg
//
LOCUS       DASTWE010000439         1362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1240100, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000439 DASTWE010000000
VERSION     DASTWE010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240100"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFT30_04640"
     CDS             complement(<1..939)
                     /locus_tag="VFT30_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5091948.1"
                     /translation="MSDSSLSPLMRQYRDIKQNYPEAILFFRVGDFYEMFYEDAQDAS
                     RLLSIALTSRDKNSDQPVPLCGVPYHAATGYIAKLLKAGRIVALCEQVEDPKAAKGLV
                     RREVVRLYTPGTLVDTEFLAHSELNYLVALAVRSGTVQSIPIGLAVLEVSTGEFWGME
                     FHGPHAVTHLLNEVARLDPREVLFPADLVRSSSAWIQQITGARLCERPPASFSQQSAT
                     RTLIEHFRVQSLEAIDCADFPAANSAAGAVLSYFRETQPTVPLSHVRRFTVRHHSEYM
                     HLDGVTIRNLELLKPLVSNEATAVVSPTTLLSVLDRT"
     gene            complement(932..>1362)
                     /locus_tag="VFT30_04645"
     CDS             complement(932..>1362)
                     /locus_tag="VFT30_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEU5091949.1"
                     /translation="SHSNRESRASNTRISPAKPPSRQKDFAAMTRLILVGILLLLSLA
                     FFLQNQEQEVTLRYFFGLLEASTPIYKPIMAGFAIGLLVAAILLFPPWVRGRIDLRRK
                     TKALQEAEVNLERLRQSLEKLSGRTQGPTQMESSGDLADE"
BASE COUNT          328 a          351 c          395 g          288 t
ORIGIN      
        1 ggtccgatcc aatacactca ggagagttgt agggctcaca accgccgttg cttcgttcga
       61 caccaatggc ttgagaagct ccagattgcg gatagtcact ccgtccaggt gcatgtactc
      121 gctatgatgc cggacagtga atcggcgaac gtgactcaga ggaactgtcg gttgagtctc
      181 gcggaagtac gacaagaccg ctcctgcagc gctattggct gctggaaagt cggcacagtc
      241 tatagcttcc aagctttgca cgcggaagtg ttcaatcaag gtgcgagtgg ccgattgttg
      301 gctgaacgac gctggtggtc gttcacacag gcgtgctcca gtgatttgct ggatccaagc
      361 ggaggatgaa cggacaaggt ctgcgggaaa gagcacttcc cgagggtcca gccgagcgac
      421 ctcattcaat agatgtgtaa ccgcgtgggg accgtgaaac tccatacccc aaaattcgcc
      481 ggttgacacc tcaaggacgg ccaggcctat cggtatgctt tgaaccgtgc ctgatcgaac
      541 agcgagagcg acgagatagt taagctcgga atgggcaaga aattctgtgt cgacgagcgt
      601 gccgggcgta taaagcctga cgacttcacg ccgcaccaga ccttttgccg ccttgggatc
      661 ctcaacttgc tcgcacaacg cgacgatccg gccggccttc aatagttttg cgatatagcc
      721 tgttgccgca tgatagggaa cgccgcatag tggaacaggc tggtcgctgt tcttgtcacg
      781 agaagtgagg gcgatcgata agagtcgaga agcgtcttgt gcgtcctcgt agaacatctc
      841 atagaaatct ccgacccgga aaaacagtat ggcctcgggg tagttctgtt tgatgtcccg
      901 atactgccgc atgagcgggc tcaggctcga gtcactcatc agcaagatcc cccgaagact
      961 ccatttgagt aggaccctgg gttcgtccgg acagcttttc aagagattgc cgaagtcgct
     1021 ccaagttcac ttcagcttcc tgtaaggcct tcgtctttcg tcggagatcg atacgcccac
     1081 gtacccaggg aggaaacaac aagatagccg caaccaaaag gccgatggca aagccggcca
     1141 tgatgggttt gtaaatggga gttgaggctt cgagcaaccc aaagaaatag cggagcgtga
     1201 cttcctgttc ctggttttga agaaagaagg ccaacgaaag aagcaaaaga atgccgacca
     1261 gaatcagtcg agtcatcgcg gcgaagtctt tctgccggct tggaggcttg gccgggctga
     1321 tccgagtgtt ggacgcacga ctttcccgat tcgagtgaga ag
//
LOCUS       DASTWE010000440         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_884739, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000440 DASTWE010000000
VERSION     DASTWE010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884739"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFT30_04650"
     CDS             complement(<1..426)
                     /locus_tag="VFT30_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5091950.1"
                     /translation="MPFINGRSIALDEKDATTVVNPANGNVVAKVFMSSPKEMTQAID
                     AAYAARESWGNTSPAEREIVLIRAAEILEAKRGEVVDVLIDEAGSTFGKAQFEVSFTV
                     NMIRATSGEARRIFGQVIPSDVPGLLSFAIRRPLGVIGGI"
     gene            complement(467..>1032)
                     /locus_tag="VFT30_04655"
     CDS             complement(467..>1032)
                     /locus_tag="VFT30_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5091951.1"
                     /translation="EEQSFQPIGAPAGVRVDVRVLAATNKKLSQEIAAGRFREDLFYR
                     LNVIPFFVPPLRERREDIPILSRYFMRMFAVEHGCTPKEFGSNALDLMTDYSWPGNVR
                     ELRNEIERLVIMVQDDTIQEKDLSLPNGGVSSTASTLHEARAQYEKEFILSKLKEYNW
                     NVSQAARALGLERSYLYRKMKTYGIEK"
BASE COUNT          218 a          289 c          297 g          228 t
ORIGIN      
        1 gataccgccg ataacgccga gtggccggcg gatggcaaag gacaagaggc cggggacgtc
       61 cgagggaatc acctggccga agatgcgtcg cgcttctcct gatgtcgcgc ggatcatgtt
      121 gacggtgaat gaaacttcaa actgagcttt gccgaaagtg gagcccgctt cgtcgatcag
      181 cacgtcgacg acttccccgc gcttggcttc caggatttca gcggcgcgga tgaggacgat
      241 ctcccgttcg gccggggacg tattgcccca gctctctctg gcggcgtaag cggcatcgat
      301 ggcctgggtc atttcctttg gcgaggacat gaagactttg gccaccacat ttccgttggc
      361 cggattgacg acggtcgttg cgtccttctc gtcgagcgcg atcgaacgcc cgttgatgaa
      421 aggcataaac tcctcctcac atgaatgctt aatctttcag aatctactat ttttcgattc
      481 cgtaagtctt cattttccga tacaggtagc ttcgctcaag ccccagcgca cgcgcggctt
      541 gcgagacatt ccagttgtat tccttcagct tggaaagaat gaactccttc tcgtactggg
      601 ctcgagcttc atgaagagtt gatgccgtag aggaaacgcc gccattcggc agggaaaggt
      661 ctttctcctg gatcgtgtca tcctgaacca tgatcacaag ccgctcgatt tcattgcgca
      721 gctcacgaac gttgccgggc caggaatagt ccgtcatcag atccagggca ttggagccaa
      781 actcctttgg ggtgcagccg tgttccacgg caaacatgcg catgaagtag cgtgacagga
      841 tcgggatatc ttcacgtctc tcgcgcaggg ggggaacgaa gaatggaatg acgttcaggc
      901 gatagaacag gtcctcccgg aagcgcccgg cggcaatctc ctgcgacagc ttcttgtttg
      961 tggcggcaag cacgcgaaca tcgacgcgaa cgcctgccgg ggcaccaatg ggctggaacg
     1021 actgctcttc ca
//
LOCUS       DASTWE010000441         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_949325, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000441 DASTWE010000000
VERSION     DASTWE010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949325"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..885
                     /locus_tag="VFT30_04660"
     CDS             313..885
                     /locus_tag="VFT30_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091952.1"
                     /translation="MTLVLAITSIIGFAGFAIFNNQSLVTKFLASHSPTSKPGVVLTD
                     SHSKPLQPDVRLTREGIDRLLDALDEAVRRKDVDGVLRHITHDATITIHLKQGPQQQM
                     ALLTREDYRKTLAMSFAFPSANDFTRTNTSVSLASDERSAKVFFKSTETLRQANREFK
                     TEGEETLVFNIRDGKPMITSLEQTFPGDST"
     gene            1210..1881
                     /locus_tag="VFT30_04665"
     CDS             1210..1881
                     /locus_tag="VFT30_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5091953.1"
                     /translation="MRVLVIEDETKVGCFIKRALEEESYAVDLCEDGAKGLEMALATN
                     YDLLVVDVMLPSMSGLDVLKNIRRERIHTPVLILSAQSQIDQRVKGLDAGADDYLTKP
                     FAIDELLARVRALLRRGASESPGILQVDDLILNPATRDVTRGGQRIDLTLKEYALLEY
                     LMRHTGRVLTRPMISEHVWNQDFDTFTNVIDVYVNYLRNKIDRGRTKKLIHTIRGSGY
                     MLKAD"
BASE COUNT          477 a          609 c          562 g          492 t
ORIGIN      
        1 tggtgcgcct gtcaatcgag taacgtatca tgctatcagc tctttttcca agagacccct
       61 ttgggagatg acttcatgga ggtcatgaca atcgccgggt tgcgaggacc ttgtgttggg
      121 ctcgcaagta ctgcgcactc agtaaatcgt ctcgcagagc ggcggcttta attgcttttc
      181 acgtaattca cgttattgtt agctcatacc gtcaacccgg agagctgcct tatggtcgcc
      241 acgaagcagg actccgatga aaggacaggg aagagatcgc cgcagcactt ctcgatggta
      301 aaggctatta tcatgactct tgttctcgct atcacatcga taatcggatt tgcgggattt
      361 gcgattttca ataaccagag tctggtaacc aaatttctgg cttcccattc accgacgagt
      421 aagcccggcg tggttctcac agattcgcac tcgaagcccc ttcagccaga tgtccgtctg
      481 actcgtgaag ggatcgaccg tcttctcgac gcactggacg aagctgttcg acgaaaggat
      541 gtcgacggcg tcctgcgtca cattactcac gacgcgacca tcaccattca tctgaaacaa
      601 gggccgcaac aacagatggc cctgctgacg cgggaagact accggaagac cctcgccatg
      661 agtttcgcct ttcccagtgc caatgacttc actcgtacga atacgtccgt ttcattggcc
      721 tcggatgagc ggagcgcgaa agtcttcttc aagtcgacgg agacgcttcg acaagccaat
      781 cgggaattca agacggaggg cgaagaaacg ctggtcttca atatccgtga cggcaagccc
      841 atgattacct ctctggaaca gaccttcccc ggcgattcaa cctagctcgc attatccatg
      901 agcgtttctg ctgcaatcac ggattcgctg ctgcgacgag ccttcggcgg gcagacttgc
      961 ccctcgcaac ctcggggctc catgtgccaa ggcactgccc gctcaccact cgcgggcgtc
     1021 cacacacgtc gcgcattatc cttcgcgccg tggacctcgc cacgcgaccg cgcgatttcg
     1081 atacgaaccg tcatgaataa tgcgggctag ccccgggatc gtgagcgatt gaacgtatct
     1141 gtacgctgca agattcatat tcatcggtct ggtataatca cgccgctaat aacatctgtt
     1201 ggagtgtgta tgcgtgtgct tgttatagaa gatgaaacca aagtcggctg ttttattaag
     1261 cgagcgcttg aagaagaaag ctatgccgtc gacctctgtg aggacggggc caagggttta
     1321 gagatggccc tggcgaccaa ctacgacctc ctggtcgtcg acgtgatgct gccgtccatg
     1381 tccggtctgg atgtgctgaa gaatatccgc cgggaacgga tccacacacc ggtgctaatc
     1441 ctctctgctc aatcccagat cgaccagcgg gtcaaaggac tcgatgccgg tgccgatgat
     1501 tacttgacca aaccgttcgc gatcgatgaa ctcttggcac gcgttcgggc cctgttgcgc
     1561 cgtggggcat ccgaaagccc gggaatcctt caagtggacg acctgattct caacccggcg
     1621 acccgtgacg tcacccgggg agggcaacgc atcgatttga cgctgaaaga gtacgcgtta
     1681 ctcgaatatt tgatgcgcca taccggccgc gtgctcaccc gccccatgat ctccgagcac
     1741 gtgtggaatc aagacttcga taccttcact aacgtcattg atgtctatgt aaattacctt
     1801 cggaataaaa ttgatcgcgg ccgaaccaag aaattaatcc ataccattcg cggtagcggg
     1861 tatatgctga aggccgacta gtacgatgcc gaagccgcct tcactcccgt gcttcggccg
     1921 cgtgtgcgct aacagaaggc tttgcgacgg tatcgtttcc tcatcagcga ccaggaactg
     1981 acctatgccg ctacgtgttc ggctgaccct ctggtacggg acggcccttg ccctggtctt
     2041 gatgattttc tccgtgattc tgtatgccat gacagcacgg aatttacgcg acgccgtgga
     2101 tcaatccctg gaagaaacgg cgactacggc tgtgcgctcg
//
LOCUS       DASTWE010000442         1434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1531090, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000442 DASTWE010000000
VERSION     DASTWE010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531090"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1288
                     /gene="fabF"
                     /locus_tag="VFT30_04670"
     CDS             38..1288
                     /gene="fabF"
                     /locus_tag="VFT30_04670"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5091954.1"
                     /translation="MSDQAARRVVVTGLGMVTPLGTGVEKTWKAICAGESGINRITRF
                     DPTGYDAQIAGEVKDFDPAQFIEKKEIKKMDTFIHYAVGAAQLAVDDAGLKVAPEEAT
                     KVGVYIGSGIGGLGSIEHYHDVLKGKGPGRVSPFFIPMTIINLASGQVAIRIGAKGPN
                     SCAVTACATGNHCIGDAYRLIQRGDADVMVAGGAEAAVTPLGVAGFAAAKALSFRNDQ
                     PTKASRPFDKDRDGFVLGEGAGVIVIEELEHALRRGVRMYGEIIGYGMNSDAYHITAP
                     PEEGEGAIRCMELALKDAGINRDQIGYINAHGTSTMADAIETRAIKRVFGEQAFRIPI
                     SSTKSMTGHLLGAAGGIEAVFSLLALFHGMLPPTINLDNPDPACDLDYVPNKARPAAI
                     TTALSNSFGFGGVNACLIFKRLDA"
BASE COUNT          322 a          359 c          440 g          313 t
ORIGIN      
        1 gaaggcgttg gactacatca aggaaaagtc ctagacaatg tcggatcaag cggcacggcg
       61 cgttgtggta accggtcttg ggatggtgac accgctgggc acgggtgtcg agaagacttg
      121 gaaggcgatc tgcgccggcg agtccgggat caaccgaatc accaggtttg atccgacagg
      181 atatgatgcc caaattgcgg gtgaagtgaa agattttgat cctgctcaat tcatcgaaaa
      241 gaaagagatc aagaagatgg atacgttcat ccactatgcc gtgggcgccg cccagttggc
      301 ggtggatgat gccgggctca aggtagcgcc ggaagaggcc acgaaagtcg gcgtgtacat
      361 cggctctggg atcgggggcc tcgggtcgat cgagcactac catgatgtgc tcaaaggcaa
      421 ggggcctggc cgagtctcac cgtttttcat tcccatgacc atcatcaatt tggcgtccgg
      481 gcaggtggcg attcggatcg gagccaaggg gcctaactct tgtgcggtga cggcttgcgc
      541 cacgggcaat cattgcattg gagatgcgta ccgcctgatt caacgaggtg atgccgatgt
      601 catggtggcc ggtggtgccg aagcggcggt cactccactg ggtgtcgcgg gatttgcagc
      661 ggccaaggca ttgtcgttcc ggaatgatca accgacgaag gcgagccgcc ccttcgacaa
      721 ggaccgagat ggatttgtgt tgggcgaagg cgcgggggtc atagtgattg aggagctgga
      781 acatgctctt cggcgtgggg tcagaatgta cggcgagatc attgggtacg ggatgaacag
      841 cgatgcatac cacattacgg ccccgccgga agaaggcgag ggggccattc gttgcatgga
      901 attggccctt aaggatgccg gaatcaatcg cgatcagatc gggtatatca atgcgcatgg
      961 cacgtcgacg atggccgatg ccattgagac ccgagcgatc aaacgggtgt tcggcgaaca
     1021 ggcatttcga attcccatca gttcgaccaa gtccatgacc ggacatttac tcggagctgc
     1081 cggtgggatc gaagccgtct tcagtcttct ggcgctgttt cacggtatgc tccctcccac
     1141 gatcaatctg gataatcccg atccagcctg tgatttggac tatgttccca ataaggcacg
     1201 acctgccgcc attaccacag ctttgtcgaa ctcctttgga ttcggtggag tgaacgcctg
     1261 tttgattttc aagagactgg acgcgtagtg cgcgtgtcac gatgacgccg gcttcttcag
     1321 ccgattcttc tcctgccata tcgaactatc gattcacgaa tccaagcttc ttgatagagg
     1381 ctttgacaca taaatcgtac gtgaatgagc gccgagactc cgttcgaaaa cata
//
LOCUS       DASTWE010000443         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_852462, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000443 DASTWE010000000
VERSION     DASTWE010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852462"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFT30_04675"
     CDS             complement(<1..615)
                     /locus_tag="VFT30_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091955.1"
                     /translation="MDRLKRCWKWFSAQSVFNKIVIIGLFAAFVPLVLPTVVAWFRPT
                     MIHVGVAFYTYERGVQPHGMNSLYFFERRNLVSDCSIRHEAQSVPSGRAKAPDPQAWV
                     LIKLLLENVSDRDITNLRVGVRSPAINQTTQLLTAPTLVATGSKEAPPDVKPIYVISI
                     ATIPGTSSAVVSLKTPIDEDLRDFIYVKRRPVTIQIPYVSADQFR"
     gene            848..2380
                     /locus_tag="VFT30_04680"
     CDS             848..2380
                     /locus_tag="VFT30_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HEU5091956.1"
                     /translation="MKYLIMLIYVSILTLAGCSHTIPPAATKPQPPAQLGSPAPAVPP
                     ISAGVKQAEAPDSSLPVTVTADLSPLSRIIQAALPARLTEEDHPLGYDYIWRFVREGE
                     PQVQIHDGIVKYRATYRGAIESPAARACRLDPLYPVIEGTGRLSFREQDKGLLVTLID
                     SRTTIDLKPESDSSCNMFKIPVKDQLAEILDRDTITQQIVQSVDRAAYTIPLSLVWER
                     LQQPITVSQANTQFCLYGRVREFIVGSMKGPVQHTTIAGMAKQTPVALYQTPCGQSKE
                     SPPMKVHMDRSIVAAEQGQPYKILLTVPVPYNILSQQLQQRLFHQAVKLPNMLGDNFL
                     IEQVTASNANGQTLLAINTSGSVNGTLYYWGAPRLERDGNVIAISDLQMAAETKTALD
                     KVKPGYWQMVDQELKARLKEAVTIDLSQQLGDVKSALSRQHKNGWLELDLLVAHQEPA
                     QVTSTQNALVADIVLEGTASATGQLPIQQRTQQSPLRGTQAPQATRAPDEENIEELPN
                     AP"
BASE COUNT          611 a          690 c          632 g          492 t
ORIGIN      
        1 tctgaactga tccgcggaga catagggtat ctggatagtc acagggcggc gtttgacata
       61 gataaagtcg cgcaagtctt catcgatagg ggtcttcagg ctgacaaccg ccgagctggt
      121 ccccggaatg gtcgcgatcg agatgacata tatgggtttc acatcgggcg gagcctcctt
      181 gctgcccgtg gcgaccaagg ttggggcggt caacagttgc gtcgtctgat tgatagcggg
      241 agacctcacg ccgactcgga ggttcgtgat atcgcgatcg gacacatttt ccaacagaag
      301 cttgatcaat acccaagctt gcggatcagg agccttcgcg cgaccactcg ggacggattg
      361 cgcctcatgg cggatactgc aatccgatac cagattgcgc ctttcgaaga aatagagtga
      421 attcatgccg tgtggctgga ctccccgttc ataggtatag aaggccaccc cgacgtgaat
      481 catggttggc ctgaaccagg ccacgacagt cgggaggacc aatggaacaa aagctgcgaa
      541 cagtccaatg atgacgatct tattgaagac actctgagcc gagaaccact tccagcaccg
      601 cttcagccta tccatggaga cctcaccaag tgttttacca cctggcacat cctaaaacat
      661 aggttcccac catcacatcg caactgcggt acaggacaaa gtgtcccgcc tggaatggct
      721 tgagctggat cgagtaaccc ccaagagatg aactatgcgt agataggagt gatctggaga
      781 atatttggtc ggtacatggc ttgcactctc tcgaacatta tactctcgat actctcggag
      841 gtgttgtatg aaatatctca tcatgctcat ctatgtctcg attctcacct tggctggctg
      901 cagtcatacc attccgcctg ctgcgacgaa acctcagccg ccagcccaat tgggaagtcc
      961 cgctccggca gtcccgccga tttctgcagg ggtaaaacag gccgaggctc cggactcttc
     1021 gctccccgtg actgtgacag cggatctgtc gccgttatcg cgcatcattc aagcggcgct
     1081 tcctgcacgt ctcacggaag aggatcaccc gcttggatac gattacatct ggcgcttcgt
     1141 acgagagggt gagccacaag tacaaatcca cgacgggatc gtgaaatacc gcgccactta
     1201 caggggggca attgagtcgc ccgctgcacg cgcctgccgg ctcgatccgc tctatccggt
     1261 gatcgaagga accggacggc tttcgttccg ggaacaggat aagggattgc tcgtcacact
     1321 gatcgattcc cgaacgacga tcgacttgaa acctgaaagc gacagcagct gcaacatgtt
     1381 caaaatccca gtgaaggacc agcttgccga aatacttgat cgggacacga tcacgcagca
     1441 gatcgttcaa tcggtcgacc gagccgctta tactatcccg ctgagtcttg tatgggaacg
     1501 actgcaacag ccgattaccg tcagccaagc caacacacaa ttttgcctct atggccgagt
     1561 gagggaattc atcgttggct ccatgaaagg cccggtacag cacaccacga tcgcgggcat
     1621 ggccaaacaa acccccgttg cgctctacca gactccttgc ggacaatcca aagaatcgcc
     1681 tccaatgaaa gttcacatgg atcgatccat cgttgccgca gagcaaggtc aaccctataa
     1741 aatcttgctt acggttcctg tgccctacaa catactcagc cagcagttgc agcagcggct
     1801 gttccatcaa gcagtcaaac ttcccaatat gttgggggac aactttttga tcgaacaagt
     1861 aaccgcctcc aatgcgaatg gtcagacgct cctggcgatc aacacgagcg gaagcgtgaa
     1921 cggtacgttg tattattggg gtgcgcctcg cctcgaacga gacggcaacg tcatagcaat
     1981 ttctgatctc caaatggcgg ctgaaacgaa aacggcgctg gacaaggtaa aaccaggcta
     2041 ttggcaaatg gtggatcagg aattgaaggc gcgcctgaag gaagctgtca ccatcgatct
     2101 atcgcaacag cttggggacg tgaaaagcgc gctaagtcga cagcataaga atggctggct
     2161 ggagctcgat ctactcgtgg ctcatcaaga gccggctcaa gtcacgtcaa cccagaatgc
     2221 cctcgtggcc gacattgttc tcgaaggcac cgccagtgct acgggacaat taccgatcca
     2281 gcaacgaaca caacagtccc ccctgcgagg cactcaagcg cctcaagcca cgcgcgcccc
     2341 agatgaagaa aatattgaag aactgccgaa cgccccgtag acacgaagcc tgatcacggc
     2401 ttcccacggc ttcaggccgc aagcg
//
LOCUS       DASTWE010000444         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1369665, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000444 DASTWE010000000
VERSION     DASTWE010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369665"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFT30_04685"
     CDS             <1..263
                     /locus_tag="VFT30_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091957.1"
                     /translation="LATQVEADQATAAPTAEPDLLGDTPPVDESVSKEKTAKESRLKK
                     LLKKTTSKSDQIALFTGEAGAKKKPGGRSTKPTKAAAPRKPK"
     gene            270..1373
                     /locus_tag="VFT30_04690"
     CDS             270..1373
                     /locus_tag="VFT30_04690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU5091958.1"
                     /translation="MTTKTKKTTLVEKKLIGLADIVVQAAERSKDPTFQIPIRALSNV
                     SFNERKGLIEMGSKKQERSFFNVGMAKKFMQTVLVADALSELQRADLTTSLREIYYRT
                     KHTIKDSHENTFDTQDESDPVIEDLEVSLAALREELHVRAENSGSIVGPVVFGDDGDR
                     VDCSKLGKGGYSVPSIVEPEYLEIRRCTADFVLLVEKGTQWNRLSEDKFWRRYNCILL
                     TGNGQPPRGVRRLARRLHEEHRLPVYVLVDNDPWGYYIYSVIKQGSINLAFESQRMAI
                     PKAKFMGFSSADPERYELPRNVGIKLNDKDIARAKELMNYQWFQKPAWQAEIKRMLTS
                     GLKYELDALANKDFQYLTKKYLPKKLQERDWLD"
     gene            1413..>2893
                     /locus_tag="VFT30_04695"
     CDS             1413..>2893
                     /locus_tag="VFT30_04695"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5091959.1"
                     /translation="MPAWTVPLRDWQRRALSSVCTHQSKDFLAMATPAAGKTRFALRV
                     AHEFMTKQAVVRVLVVCPTNHLRTQWSDAAGKIGLQLDPTLTNEQAAEAPDYHGAVVT
                     YQQVCLAPASFQRVCKSKKTLLIFDELHHAGDGKNWGKALRSAFDPAVFRLILSGTPF
                     RSDNNPIPFIRYENGESRADFAYGYTDAIRDSVCRPIVFPSYEGELSWLSDGREHQAT
                     FEDGLTFNRQRERLKTALLQETWLGPVITDAHAQLTRLRKQEQPDAGGLIVCMDQEHA
                     RWVADLVGRSVGTKAAVAVSDDPAASRTIEEFADHKKQQWLVAVNMVSEGVDIPRLRV
                     GVYGTNVITEMYFRQVVGRFVRMQEGLPKPQRAWLYLPKDPVLVHYAKQIKAERDHVL
                     EDLLPAEQRDLFGRVTVSTNEYMPLMAVARMDSLIGEDETRGEGDVTAVTEPAISLHE
                     QKQDLRDLHRLLVSTVARNSGIDHRRLNAELIARTGSRVDQATT"
BASE COUNT          687 a          766 c          873 g          567 t
ORIGIN      
        1 aattggccac tcaggttgaa gccgatcagg cgacagccgc gcccacagct gaaccggatt
       61 tgcttggcga cacaccgcca gttgatgagt cggtttcgaa agagaaaacc gccaaagaaa
      121 gtcgcctgaa aaaattgctg aagaagacaa cctcgaaatc agatcagatc gcgctcttta
      181 ccggtgaggc cggagcgaag aagaaacctg gtggaagatc cactaaaccg accaaggcag
      241 cggctccgcg caaaccaaag taggacatca tgacgaccaa gacgaagaag acgaccctgg
      301 tcgagaagaa actgatcggc ctggcggata ttgtggtcca agcggccgag cgatcaaaag
      361 acccgacgtt tcagatcccg atccgagctc tctcgaatgt ctccttcaac gaacggaagg
      421 gtctcatcga gatgggcagc aagaaacagg aacggtcgtt ttttaacgtc ggcatggcga
      481 agaaattcat gcaaacggtg ctggtggccg atgctctctc cgaattgcag cgggccgacc
      541 tgaccacgtc ccttcgagaa atttattacc gcacgaaaca tacgatcaaa gattcgcacg
      601 agaatacgtt cgatactcag gatgagtcgg atccggtcat tgaagatttg gaagtgtccc
      661 tcgccgcgct tcgggaagag ctgcatgtcc gtgccgagaa cagcggcagc atcgttgggc
      721 cggtggtctt cggcgatgac ggcgatcgtg ttgactgttc gaagctcggg aaaggtggct
      781 actcggtgcc gtcgattgtg gaaccggagt atctggagat ccggcgctgc acggcggatt
      841 tcgtactgct cgtggaaaag ggaacgcagt ggaaccggtt gtcggaggac aagttctggc
      901 gtcgctataa ctgtatcctc cttacaggaa acggtcagcc cccacggggt gtgcgccgct
      961 tagccagacg gctgcacgag gaacaccggc tgccggtcta tgtcttggtc gacaacgatc
     1021 cgtgggggta ctacatctat tccgtcatca aacaaggctc gatcaatctt gcttttgaaa
     1081 gccagcggat ggccattccg aaagccaagt tcatgggctt ttcgagtgct gacccggagc
     1141 ggtacgaact gccacggaat gtcggcatca agctcaatga taaggatatc gcgcgggcga
     1201 aagagctgat gaactatcag tggttccaga agcccgcctg gcaagcggag atcaagcgca
     1261 tgctgacgag cggattgaaa tacgaactgg acgcgttggc caataaagac ttccagtacc
     1321 tcaccaaaaa atacctaccg aagaaacttc aggaacggga ttggctcgac tagcctccga
     1381 gaaccggtag ccacgcaccg gctatgaata tcttgcctgc ctggactgtg ccccttcgtg
     1441 actggcagcg gcgagctctg tcgtcggtct gtacccatca atcgaaagat tttctggcga
     1501 tggcgacgcc tgcggcaggg aagacgcgtt ttgcactacg cgtcgcgcac gagtttatga
     1561 cgaaacaggc agtggtcaga gtgttggtcg tctgtccgac gaatcatctc cgcacgcaat
     1621 ggtcggatgc agcggggaag attggtttgc agcttgatcc gaccctgacc aacgaacaag
     1681 cggcggaagc gccggactat cacggcgccg tggttaccta ccagcaggtc tgtctggctc
     1741 cggcaagttt tcagcgagtc tgcaagagca agaagacctt gctcatcttt gacgagctgc
     1801 accacgctgg cgacggcaag aactggggga aggcgctgcg ctcggcgttt gacccggcgg
     1861 tgttccgatt gatcctgtcc ggcacgccgt ttcgttcaga caacaatccg atcccgttta
     1921 ttcgatatga gaatggagaa agccgggcag atttcgccta cgggtatacc gacgcgatcc
     1981 gcgacagcgt ctgccggccg atcgtcttcc cgagctacga aggcgagttg agctggttat
     2041 ccgatggccg cgagcatcag gcgacatttg aggacggact gacgttcaat cggcaacgcg
     2101 aacgactgaa gacagccttg cttcaggaaa cctggctcgg gccggtgatc accgatgccc
     2161 acgcccaatt gacccgcctt cggaagcagg aacaaccgga tgccggtgga ttaatcgtat
     2221 gtatggatca ggaacatgca cggtgggttg cggatctcgt tggtcgtagt gtcggcacca
     2281 aagcagccgt tgccgtgtcg gacgacccgg ctgcttcgcg cacgattgaa gagttcgccg
     2341 atcataaaaa gcagcagtgg ctagtggcgg tcaacatggt gagcgaaggc gtggatattc
     2401 cgcgactccg tgtcggtgtc tatgggacga acgtgatcac ggaaatgtat ttccgacagg
     2461 tcgtcggccg gttcgttcgg atgcaggagg gtctgcctaa accgcaacgg gcgtggctgt
     2521 atctgccgaa agacccggtc ttggtgcact atgccaagca aatcaaggcc gagcgcgatc
     2581 atgtgttgga agacctcctg ccggcggagc aacgagacct cttcggccgc gtcacggtct
     2641 cgacgaacga gtacatgccg ttgatggccg tggcaaggat ggatagcctg atcggtgaag
     2701 acgagacgcg aggggagggc gacgtgactg ccgttaccga accggctatc tcgcttcatg
     2761 agcagaaaca agacctgcgg gatctccacc ggttgcttgt cagcacagtc gctagaaaca
     2821 gtggaatcga tcatcgacgt ttgaatgctg aattaatcgc gcgcaccggg agccgtgtcg
     2881 atcaggcgac gac
//
LOCUS       DASTWE010000445         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1207903, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000445 DASTWE010000000
VERSION     DASTWE010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207903"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFT30_04700"
     CDS             complement(<1..376)
                     /locus_tag="VFT30_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091960.1"
                     /translation="MHDLIYCFRMLRKRPSFTIVAVLTLALAIGASTALFSIVNIVVL
                     NPFPYKDPSRLFFVRQALPKIGVQEQLRLSGPEFVDLTNSGIFERVAAVETVSRNLTG
                     SDEPERVAAAKVSAEFFNILGIE"
     gene            800..>1052
                     /locus_tag="VFT30_04705"
     CDS             800..>1052
                     /locus_tag="VFT30_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091961.1"
                     /translation="MSVNSTGTGSGNARSGDFGVYRITPDGRYVLFYSEASDLVPIGA
                     TRASDIFVRDLQTGQTKMVSINMTGTPSTQSSPFGLISDN"
BASE COUNT          251 a          292 c          280 g          229 t
ORIGIN      
        1 gttctatgcc gaggatgttg aagaactcgg ctgacacttt ggctgctgcg actcgttcgg
       61 gctcgtcact gccggtgaga ttgcgactaa cagtctcgac tgcggcgaca cgttcaaaga
      121 tcccactgtt cgtcagatct acgaactcgg gtcctgagag tcggagttgt tcctgcacgc
      181 cgatcttggg cagagcctgg cgtacgaaga acagtcgtga aggatctttg tagggaaacg
      241 gattgagcac cacgatgttg acgatgctga agagcgcagt gctggcgccg atggcgagcg
      301 cgagagtgag cacggccacg atggtaaagg agggccgctt acgcagcata cggaagcagt
      361 agattagatc gtgcatacag agagattcct ctcggcgtga tctcgtaggg cgcccgtgat
      421 acctcaaagc ccgtgccaga ctgagaagag gagatctgca cgagaatcaa aataaataat
      481 atgcattctg agttcttgga gtttcacttg cgggacttac gatcccactt tcgaacattt
      541 atttaattgg aaatcacggg gcgaactgta ggggcggccc tccgtggccg ccccatcatg
      601 aaaaggaagg ggtggccacg gagggccacc cctacaattg gaccaatctt gacaacactg
      661 cccaacgtca ctactctcac gccgtccgct gagttcacac cgtctcccct atcaaaatat
      721 gaagcacttc ctcctattca tttctactat ctgtttgttc gtccagtcgg ctgtagcaca
      781 gcaaggacag ctatctctcg tgagtgtcaa cagcaccggc actggcagcg gaaatgcacg
      841 ttccggtgat ttcggcgtct atcgcatcac gcccgatgga cgctacgtac tcttttacag
      901 cgaagcaagt gacctcgtac cgatcggcgc gacccgcgca tctgacattt ttgtgcgtga
      961 cctccaaacg ggccaaacca aaatggtcag catcaacatg accggcacgc cctcgaccca
     1021 aagttcaccc tttggcctca tcagcgacaa cg
//
LOCUS       DASTWE010000446         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1143495, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000446 DASTWE010000000
VERSION     DASTWE010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143495"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1132
                     /locus_tag="VFT30_04710"
     CDS             <1..>1132
                     /locus_tag="VFT30_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5091962.1"
                     /translation="AFSIECKTGERVVLIHEIEKRVPESNLAEVVNCIRRALADEYEL
                     EIHSVVLIKPGTIPRTSSGKIQRGACKAAFESGQLAVVEASTLDPILETETDGVPSEA
                     LQTAIEKRLADIWQEVLGGHSPHRQANFFALGGNSLLAAQVVARILDVFHVELPLSVL
                     FECPTLSTLAGRIGEPSMSLDDPDQYANSDGRTRIPSVPLASTSIREGKIPLSPSQQR
                     LWFLEQVHPGSAINHISMEVRLRGSVNPEVVERSVQEIVRRHEILRTRFGSERGEGFA
                     EVSSEAIVTIGRQDFQALDPAEQEMQIRQCLQAKRSQPFDMRRGPLFRVTLLALEQDV
                     HILALTFHRLVADGWSLRIFWRELALLWEAGGYVQGAGLPTLT"
BASE COUNT          250 a          299 c          336 g          247 t
ORIGIN      
        1 ggcgttctcc atcgagtgca agaccggaga gcgtgtggtc ctgatccatg agatcgagaa
       61 gcgggtgcct gaatcaaacc tggcggaagt ggtgaactgc atccgtcgtg cgttggcaga
      121 cgaatatgaa ctcgaaatcc atagtgtggt gctgatcaaa cccggcacga ttccaagaac
      181 ctccagcgga aaaattcagc ggggcgcttg caaagccgcc tttgagtccg gccaactcgc
      241 tgtagtcgaa gcgagcacgc tggacccgat ccttgaaact gaaaccgatg gagtgccgag
      301 cgaagccctg caaacagcca tcgagaaaag gctggctgat atctggcaag aagtgcttgg
      361 aggacattct ccccatcgtc aggcaaactt ctttgcactc ggaggcaatt cgctcctcgc
      421 agcacaggtt gttgcgcgca tcctcgatgt cttccatgtc gagcttccgc tcagtgtgct
      481 gtttgaatgt ccgacattgt ccacactggc tggtcggatc ggtgaaccga gcatgagtct
      541 ggatgatcca gaccagtacg caaatagcga cggccggacg cggattccat ctgttccgct
      601 tgcctcgaca tccataagag aaggcaagat tcctctcagc ccctctcagc aacgactgtg
      661 gttcttggag caagttcatc ccgggagcgc gatcaaccat atttccatgg aggtgcgttt
      721 acgggggtcg gtcaaccccg aggtggtaga gcggtcggtg caggaaattg ttcgccgaca
      781 cgagattctc cgaactcgtt tcggatcgga gcgaggcgag ggattcgcgg aagtttcctc
      841 tgaggcgatc gtcacgatag gacggcaaga tttccaagca ttggaccctg cagagcaaga
      901 aatgcagatt cggcaatgct tacaagcgaa gaggagccag ccgtttgaca tgcgccgggg
      961 tccgttattc agggtgaccc tgctggcgct tgaacaggac gtgcatatcc tggcgctgac
     1021 attccaccgt ctcgtcgccg atgggtggtc tcttcgtatc ttctggaggg aattggcgct
     1081 tctttgggaa gccggtgggt atgttcaagg agctggactt cccacgttga cc
//
LOCUS       DASTWE010000447         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_949538, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000447 DASTWE010000000
VERSION     DASTWE010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949538"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..786)
                     /gene="nuoI"
                     /locus_tag="VFT30_04715"
     CDS             complement(220..786)
                     /gene="nuoI"
                     /locus_tag="VFT30_04715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU5091963.1"
                     /translation="MASTTTTKRLNLYEWFKTITFYEILVGMKATLSHLLNYRPVTLQ
                     YPHEKRTLPDNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEPTKRY
                     SKEYYMDMTRCLFCGMCVDACPVDALGMTREFEWAVYDKRQLHLNKQQLLAIGDRSFP
                     VREKRLELQHPNVAFFNVAFKHVPPKPD"
     gene            complement(801..1877)
                     /gene="nuoH"
                     /locus_tag="VFT30_04720"
     CDS             complement(801..1877)
                     /gene="nuoH"
                     /locus_tag="VFT30_04720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU5091964.1"
                     /translation="MTEFGLRLAISLTQIAAVMGVVVVTVLILTLAERKVLGWMQDRM
                     GPMEVGPYGILQPFADAIKLFFKEDIIPAGANKFLFTIAPILCLIPAFIGFAVIPWGP
                     NQTFEVDGITVRPFVISDINIGILYILAFASLGAYGIILGGWASNSKYSLLGGLRSAA
                     QIISYELNVGLSIVGVLILAGSLSLVKITEAQTGGFWNWYLFALPAPQIFAFVVYVIS
                     SVAETNRVPFDLPEAESELVAGFFTEYSGLRFAFFFLAEYANMVLVSCVAAALFLGGW
                     NAPYPGTIMALIGVPSLAWVENTMWFAVKTYSFLFLFFWLRATLPRLRYDQLMRFGWK
                     VMLPIALGNIVVTSLAVFFYQQMK"
     gene            complement(1920..>2388)
                     /locus_tag="VFT30_04725"
     CDS             complement(1920..>2388)
                     /locus_tag="VFT30_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU5091965.1"
                     /translation="SLGPTPLPPKVDRSVVARYLTAGYQRGVATRYQPIPRTSRPDGT
                     VRLELTQSLFHSGKLSTRSKGLLQIEGSGRLRINPLDAARFALSDGDRVRLSSTTGEM
                     TTEVKIMERVPQGTAWFPYHFGQDAVRLFECIIDPITHVPALRTATVSIMKVA"
BASE COUNT          599 a          635 c          661 g          493 t
ORIGIN      
        1 aagagccccg cgatatggaa gaacatgact aagagcgaca gcgcgctgta aacgggattt
       61 ctcagcgcga ccaccaggat ggaggtgatg gcgatcatcc cggcaaaata tccaaaaaac
      121 agctgcgaca tgagacggtt ccgggttaaa gggcaatcag agccggacta acgcaacggt
      181 gttgcagtca ttggtcgttc gatgagtaat attagagagt cagtccggtt ttggtggcac
      241 gtgcttgaat gccacgttga aaaacgcgac gtttggatgt tgaagttcca ggcgtttttc
      301 acggactgga aacgaacggt cgccgatcgc gagcaattgt tgtttattca ggtggagttg
      361 ccgcttgtcg tacaccgccc attcaaattc tcttgtcatg cccagtgcat cgacagggca
      421 ggcatccaca cacatcccac agaacaagca acgggtcata tccatgtaat attctttgga
      481 ataacgctta gtcggttcgc ccggtacttc agcgctgacg actcgaatga ctcgcgacgg
      541 acaggctgct tcgcagaggt cacatcccac gcacttctcg gtcccatcat cgtatcggag
      601 cagcgcgagc atgcctcgat aattgtccgg cagtgtgcgt ttttcgtgag ggtattgcaa
      661 ggtcacaggg cggtaattga gtagatgcga caatgtcgcc ttcatgccga cgagaatctc
      721 gtagaacgtg atcgttttga accattcgta caggttcaaa cgcttggtgg tcgtggtaga
      781 tgccatatcg gtctcgtccg ctacttcatc tgttgataga aaaacaccgc aagggacgtc
      841 acgacgatgt tacccaacgc aatgggcaac atcaccttcc acccgaatct catcagctgg
      901 tcgtatcgca gcctcggtaa cgtggcccga agccagaaaa agaggaacaa gaaagagtac
      961 gtcttgaccg caaaccacat cgtattctcg acccaggcca aggacggcac accgatgagc
     1021 gccataatcg ttccaggata cggggcgttc catccgccga gaaataacgc cgcagccacg
     1081 caggatacca gcaccatgtt ggcgtactca gcgagaaaaa agaaggcaaa tctgaggccg
     1141 ctgtattcag tgaagaaacc ggcgacgagc tcgctttccg cctccggcag atcgaacggg
     1201 actcgattgg tttccgccac cgatgagatc acgtaaacga caaaggcaaa aatttgcggg
     1261 gccggtaacg cgaagagata ccaattccaa aagcccccgg tctgagcctc ggtgattttc
     1321 acaaggctga gtgaaccggc cagaatcaac acgccgacaa tggacaatcc gacattgagc
     1381 tcgtaactga tgatctgtgc cgccgatcgt agaccgccga gcagcgagta tttactgttg
     1441 gaagcccatc cccccaggat gatgccgtag gccccgagcg aggcgaacgc taagatgtac
     1501 aggatgccga tattgatgtc gctgatcaca aacggcctca cggtgatgcc gtcgacctca
     1561 aatgtctggt tgggacccca gggaattacg gcaaatccga tgaacgcggg aattaaacaa
     1621 agaatcggcg caatggtgaa aaggaacttg ttggcgccag cgggaataat gtcctccttg
     1681 aagaagagct tgatggcgtc cgcaaatggt tggagaatgc cgtaggggcc cacttccatc
     1741 ggccccatgc gatcctgcat ccatcccagg acttttcgtt ctgcaagggt gaggatgagt
     1801 accgtaacga ccacgacgcc catgaccgcg gcgatctggg taagggagat agcgagacgc
     1861 aatccgaatt cagtcacaat tgggctcctc gattagcgca tcaacgatga agagaatcat
     1921 cacgccacct tcatgatgga caccgtcgcc gtgcggagcg ccggcacatg ggtgatcgga
     1981 tcgatgatgc attcaaatag tcggacggca tcttggccga agtgatatgg gaaccatgcc
     2041 gttccttgcg ggacccgctc cataatcttg acctcagtcg tcatttctcc cgtcgtgctg
     2101 gagaggcgga cccgatctcc atccgacaag gcaaagcgtg cggcgtcaag cggattgata
     2161 cggagccggc cgcttccttc gatctgtaac aatcctttcg atcgtgtgga taatttccca
     2221 gaatggaaca ggctttgtgt caattccagc cgcacggttc catccggtct ggacgttcgt
     2281 gggataggtt ggtacctcgt cgcaacgcca cgctgatatc cggcagtgag gtagcgggct
     2341 acaaccgagc gatcaacttt gggcggcaac ggtgttggcc ccagcgag
//
LOCUS       DASTWE010000448         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1402331, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000448 DASTWE010000000
VERSION     DASTWE010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402331"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFT30_04730"
     CDS             <1..389
                     /locus_tag="VFT30_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091966.1"
                     /translation="TVTALPFSGQPTMPRMPWVLREREIVLDLPLLHVLKDAGARPLP
                     RITVELFDKTDPELDVTSTISRINDTAVIRGTFKPPLEGDFTFVVSGNLLIGTIQMAG
                     RVYKTEHIVNGRLRLVEFDPDKMPPD"
     gene            621..2444
                     /locus_tag="VFT30_04735"
     CDS             621..2444
                     /locus_tag="VFT30_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091967.1"
                     /translation="MTTATLQTKPAPETHLQRTLNAALNEIGTDAALAAIFHQENGPL
                     IDHASRGFTPRDVQAILRTLSTQRAAALAPTVQDPDGGRVIRLRLITPGAKSLLAVPL
                     RHFIDVYGCLVIGRQEGAAFSKKDKSHLEQMCDGMTKALDREGLFNTNTVLSRSYVTQ
                     EPGPPQQTGVDLFPPMTKHFSPELQEKIEAVLTEAHQYVTYDRAWACYYDPLAGNVEV
                     LGIAGDVKTDPKDAKKDLKPGQRLTLDSSAAGWAVRHRKPRVDHDLASTQGRFLDHKH
                     LFKDRFQSSLVIPFFVKGQVGGTFTLGSKDPQRYQTTDARTLEPIILKLAELLQAPPP
                     PASVPSLTTDVGTPGPRPPVAAASEPIIRKQERQAAIVEFSAFLATEIREPLASIRSQ
                     LEEVTGEGILDFDPQTRVENAMRDLIRIEAILNEILDFAKPLELSRHLCRIPEVLESA
                     LVVVGTDLEATRIQVTKDYANVIAPVRGDEAKLQQTFLSIFRNACEAMSPGGHLHIQV
                     TQHRAGRGFEVQILIKNDGVPIPAEIVDKVFEPFFTTKSSGIGLGLPSVKKIVEEHGG
                     TIAIGSAIGEGTTVTIRLPGISRGPAFRHRGRGRRHPRRPN"
     gene            2647..3012
                     /locus_tag="VFT30_04740"
     CDS             2647..3012
                     /locus_tag="VFT30_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091968.1"
                     /translation="MKLLTAAVATVSSILLACSVASANPALLPKHEGYPMKNTGSPVN
                     GQPTANDPGQPDARGTSTLLKSADSVKNVEQRLIKTDNARITEGQGAGRLPNVEGPQI
                     QIAPPVTSATKITGDRTID"
     gene            complement(3092..>3375)
                     /locus_tag="VFT30_04745"
     CDS             complement(3092..>3375)
                     /locus_tag="VFT30_04745"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU5091969.1"
                     /translation="NVVTEPRESPAAVLIRAIEVDGELIDGPGKLCRELGIDRSLNRL
                     DMTHGQSLWFEDRGERVPSKQVGTFPRIGVDYAGVWAKKLWRFRLGGVG"
BASE COUNT          776 a         1053 c          865 g          681 t
ORIGIN      
        1 aaaccgtcac ggctcttccc ttcagcggtc aaccaacgat gccgagaatg ccctgggttc
       61 ttcgcgagcg agagatcgtg cttgatctcc cgctccttca cgtgctcaaa gatgccggag
      121 ccagacccct tcccaggatc accgtcgagc tctttgataa gaccgatccg gaattagatg
      181 tcacctccac gatatctcgt atcaacgaca ccgccgtcat ccgcggcacc ttcaagccac
      241 ccttggaggg cgactttacc tttgtggtca gcggcaatct cctgataggg accattcaga
      301 tggcggggcg ggtctataaa actgagcaca ttgtcaatgg ccgactgcgg ctggtcgaat
      361 tcgatcccga caaaatgccg ccggattgat tacgacagca tcgtgctgac tgtcaaccat
      421 gcggagaaga cgttcgagct gctcaatcat cacccgttaa gaagaagccg actgagccga
      481 ccatttgatc accgcgagaa tcttatcgcg tgtcgcgcaa acgatggtga tagctcacat
      541 caaagcgtcg caacagcccg gtgagagagt agctattcgc cgacgttccg gctacaatgc
      601 cgtcgcttgc atctgaacgt atgaccacag cgactcttca aacaaaaccg gcccccgaga
      661 cgcatcttca acgaacgttg aacgctgcgt tgaacgagat cggcacggat gccgcattgg
      721 cggccatctt tcatcaagag aatggtccac tcatcgacca tgcgtcgcgc gggttcacac
      781 cgagagatgt ccaagccatc ctccgcaccc tgtcgacgca acgggccgcg gctttggccc
      841 cgactgtcca ggatccagac ggagggcgtg tcatccgcct gcggttgatc acccccgggg
      901 caaaatcctt gctcgccgtt ccgcttcgcc acttcattga cgtctacggt tgcctggtca
      961 tcggacggca agagggcgca gccttctcca agaaggacaa atcccacttg gagcagatgt
     1021 gcgacggcat gaccaaggcg ttggaccgcg aagggctttt caataccaac acggtgttga
     1081 gtcgttccta cgtcacccag gaaccggggc ctcctcaaca aactggagtg gacctgtttc
     1141 cgccgatgac caagcacttt tcgcccgagc tccaagagaa gatcgaggcg gtcttgaccg
     1201 aagctcacca atacgtgacc tatgaccgcg cctgggcctg ctactacgat cctctggcgg
     1261 gaaacgtcga ggtcttgggc atcgctgggg acgtgaaaac agacccaaaa gatgccaaga
     1321 aagatttaaa acccggtcag cgcctgacgc tcgacagttc agcagcggga tgggccgtcc
     1381 gccatcgcaa acctcgcgtg gatcatgatt tggcttccac ccaaggtcgc tttctcgatc
     1441 acaaacatct cttcaaggac cgatttcagt cgtccctcgt tattcccttc ttcgtcaaag
     1501 gccaagtcgg cggaacattt acgttggggt cgaaagaccc acaacggtat cagactaccg
     1561 atgcccggac actggagccc attattctca aactcgcgga gctcttacag gcgccgccgc
     1621 cgccggcttc cgttccttct ctgacgaccg acgtcggaac accgggccct cgaccgcccg
     1681 tggccgccgc gtctgagccc attattcgaa agcaagaacg acaggccgcg atcgtggagt
     1741 tcagcgcgtt cttggcgacg gagattcgag agccgctcgc gtccattcgc tcgcagcttg
     1801 aagaagtgac cggtgaagga atcctcgact tcgatccaca gacccgtgtc gaaaatgcaa
     1861 tgcgtgactt gatccggatt gaagcgattc tgaacgagat cctcgacttt gcgaagccgc
     1921 tggaactcag tcgccatctg tgccgtattc cagaggtgct tgagagcgcc ctcgtggtgg
     1981 tcggaacaga cctggaagcg acccgtattc aagtcaccaa agactacgcc aatgttatcg
     2041 ctcccgtacg cggcgatgaa gccaaacttc aacagacgtt cctgagcatc ttccggaacg
     2101 cgtgcgaggc catgtctccc ggtggccatc tccatatcca ggtcacccaa catcgcgccg
     2161 gacgagggtt cgaggttcag attctcatta agaacgacgg ggtccccatc ccggctgaaa
     2221 tcgtcgacaa ggtcttcgag ccatttttca ccaccaagag ttccggaatc gggctgggtc
     2281 tccccagcgt caaaaaaatc gttgaggaac acgggggaac cattgcgatc ggcagtgcca
     2341 tcggtgaagg caccactgtc accattcgtc tccccggcat cagccgcggg ccggcgtttc
     2401 gacaccgtgg ccgtggccga cgccacccac ggcggccgaa ttgacgcgtg cctcctcttc
     2461 tccttctcac cactccggcg ctcagctact ctgataccca cagagaggga caaactgacg
     2521 atcgttcaac gtttgacaga aatagccgct catcgttatg attcagcccg tatgattggt
     2581 tgtcaaaatt gaacttacaa tattcatgat cctcaaccat cgtcatatct caaagggaga
     2641 atgggtatga aacttcttac cgctgcagtc gcgacagtct cctcgatctt gcttgcatgt
     2701 tcggtggcgt ccgccaaccc ggcgctgctg ccgaaacatg aaggctaccc catgaagaac
     2761 acgggaagtc ccgtgaacgg ccaaccgact gccaacgatc ccggtcagcc cgacgcacgg
     2821 ggaacgtcca cgctcttgaa gtcggcagat tcggtcaaaa atgtcgaaca gaggctgata
     2881 aaaaccgaca atgcgcgaat cacggaagga cagggtgccg gaaggcttcc aaacgtagag
     2941 ggacctcaga tccaaattgc tccgccggta acctccgcga ccaagatcac cggcgatcgc
     3001 acgatcgatt agcttcttcc atcatgaagg gggaaatctt tccaaggttt cccccgatca
     3061 gccgtcttcc ttacccaaca aagcgcatct cttagccaac cccgcccaac ctgaaccgcc
     3121 agagtttttt tgcccacacc ccagcatagt caacaccaat tctcgggaat gtgccgacct
     3181 gcttgctggg aacccgttcg cctcgatcct cgaaccagag ggattgtcca tgggtcatgt
     3241 cgagtcgatt caatgaccga tcgatgccta actcccggca gagctttcca gggccatcaa
     3301 tcaactcacc gtcgacttca atcgctcgaa tgagcaccgc tgcgggagac tccctcggct
     3361 ccgtcaccac gttca
//
LOCUS       DASTWE010000449         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_335704, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000449 DASTWE010000000
VERSION     DASTWE010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335704"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFT30_04750"
     CDS             <1..837
                     /locus_tag="VFT30_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEU5091970.1"
                     /translation="LDVPNANTIIVNRADLFGLAQLYQLRGRVGRGGEQAYAYFLIPD
                     EGMLTGDAQKRLIAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHIAAIGLDLYM
                     QMVEQAVQRLKGHVIEEEPDPTLQLPVSAFIPEQYVADPHHRLSLYKRLTACGQIGEL
                     ALLHGEIQDRYGPPPEPVERLLEVMQLRTHAKRLRLASIEVHGQTAKVVFHPKAVIPE
                     TSVHRLMDQLKKRLRFPSPVSFEIQMRHDDWPALFSELNAILQSLDLCDTKTIQKDAT
                     AS"
BASE COUNT          234 a          312 c          290 g          227 t
ORIGIN      
        1 ctcgacgtcc ccaacgccaa caccatcatc gtcaatcggg cggatctctt cggcctcgcg
       61 cagctgtatc agttgcgtgg acgggtcgga cgcggcggag agcaggccta cgcctacttc
      121 ctcatccccg atgaaggcat gctcaccggc gatgctcaga aacgattgat cgcgatccag
      181 caattcaccg aactcggatc aggcttccgt atcgctgcgg cggaccttga gattcgaggg
      241 gctgggaatc tgttgggcaa acaacagtct ggccatatcg ctgcaatcgg cttggatctt
      301 tatatgcaaa tggtggagca ggctgtccag cgattaaagg ggcatgtgat agaggaagag
      361 ccggacccga ctctgcaatt gccggtttcg gcgtttattc ccgaacaata cgtggcggat
      421 ccgcatcacc gcctgtccct ctacaaacgg ctgacggctt gtggccaaat cggcgaactg
      481 gccctgttgc acggggagat tcaggaccgt tacggccccc ctccggaacc ggtcgaacga
      541 ctgcttgaag tgatgcaact ccggactcat gccaaacgac tgcgcctggc ctcgatcgag
      601 gtgcatggcc agacggccaa ggttgtgttc cacccgaaag cggtcatccc tgaaacctct
      661 gtccaccgat tgatggacca gctcaagaag cggctacggt tcccgagccc ggtctcattc
      721 gagatccaaa tgcgtcatga cgattggccg gcgctttttt cagaactcaa tgcaatcttg
      781 caaagcctcg atctctgtga taccaagacc atccaaaagg acgcgactgc ctcttaacag
      841 cttgttgacc aacctacgac tagcccagat aatggagccg tgttgagatt gtcacgcttt
      901 tcaaatgccc tgcaggctgt ttagaaaggt cgtccggcaa ggccgcagcg agcgaaaagg
      961 cgaggcgtac tctttgccgt acgttgagcc tctgagcgat gcgagaacgc cgctggcggt
     1021 ctttttccgc atcctgctag agataaaact ttctgtcacc ctc
//
LOCUS       DASTWE010000450         1852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_238876, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000450 DASTWE010000000
VERSION     DASTWE010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238876"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFT30_04755"
     CDS             <1..612
                     /locus_tag="VFT30_04755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5091971.1"
                     /translation="RGLDHDDTIRDKTQRYKEQLILDELLKDKLQSKVELTQAELDAY
                     YEKHANQLLDPLKANVSVMLLPNVYAAKDLEAQVNRGGSFAKFAQRYSIDEKTKSKGG
                     ELGPYRKGLVLPEVDAVIHTLKLGMVSAPIKTDHGYYLVMLTPLDEAVIQTDLATQER
                     LRQELLSDKRRKRFEEVIADIRANAVVRFAEASRHINEDTGKR"
     gene            687..1694
                     /locus_tag="VFT30_04760"
     CDS             687..1694
                     /locus_tag="VFT30_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU5091972.1"
                     /translation="MMRHVSVGSDWLVPFALPFILLMISMWSPPCSEARLQDRIVAIV
                     NSELIMLSDVKREFEMEQERLSREHHGNDLPQRLKTAEYMALTKLIERRLQLQEAKAK
                     KVEVSDLEVKHALEQMKRQGNPLDITDTNHAQTVRDQLLLMRVVDLHIRGNITVGDSE
                     LKRFYQEHRDRFAHPEEYQLSQIIIQPRSPDGLAEALTKARRAMDHLKRGEKFEDVAM
                     QYSDGANALQGGRLGLVRQGELIPAIEQAVAHLVPGGISDIIESPEGIQIIRMDDRKP
                     KQFRQYEEVRREIQELVYQQKSEEMYQSWLVDLKNKAYIEIKFEQVADKAQLQPLHSS
                     P"
BASE COUNT          480 a          514 c          478 g          380 t
ORIGIN      
        1 cggggtcttg atcatgacga taccattcgt gacaagacac agcggtataa agagcagctg
       61 attctcgacg aattgttgaa agacaagctc cagtccaagg tcgagctcac ccaagcggaa
      121 ctcgatgcct actacgaaaa gcatgccaac caattattgg accccttgaa agccaatgtc
      181 tcggtcatgc ttctccccaa tgtgtatgcc gccaaagacc ttgaagcaca ggtgaatcga
      241 ggcggtagtt ttgccaagtt tgcacagcga tactcaatag acgaaaaaac gaaatccaag
      301 ggcggtgagc tcggacccta tcgaaaagga ctggtcctcc ccgaagtcga tgccgtcatc
      361 cacaccctta aactggggat ggtcagtgca ccgatcaaga ccgaccacgg ctattacctg
      421 gtcatgctga ccccgcttga cgaagccgtt atccaaaccg atttggcgac gcaggaacgg
      481 ctccgccaag aattgctgtc cgataagcgc cgcaaacgat tcgaagaagt catcgccgat
      541 attcgggcca atgccgtggt tcgcttcgct gaagcctccc gccatatcaa cgaggacacg
      601 ggtaaacgct gacttcactc gagttccacg aactccccgc ttcgtgtaga attatgcatc
      661 ggcactcttg gccgcgagat gaacgtatga tgagacacgt atcggtcgga tccgattggt
      721 tggttccttt cgcgttgcca ttcattctct tgatgatctc gatgtggtcc ccgccttgtt
      781 ccgaggctcg gcttcaagac cgcatcgtcg ccatcgtcaa ttccgaattg atcatgttgt
      841 cggatgtgaa gcgtgagttt gaaatggagc aagagcggct ttcccgcgag caccatggaa
      901 acgatctccc ccagcggctg aaaacagcgg agtacatggc tttgacgaag ctcatcgagc
      961 ggcgattgca gctgcaggaa gccaaggcca aaaaagttga agtctcagac ctggaagtga
     1021 agcacgccct tgaacagatg aagcggcaag gcaatcccct tgacatcacc gatacgaatc
     1081 atgcgcagac cgttcgcgat cagctcctcc tcatgagggt cgtggacctg catattcgcg
     1141 gcaacatcac cgtcggagac tctgagctca agcgattcta ccaagagcat cgtgaccggt
     1201 tcgcccatcc cgaagagtac caactgagcc agatcatcat tcaaccgcgc tctccggatg
     1261 gattagccga ggccttgacc aaagcccgcc gagccatgga ccacttgaag cgaggtgaga
     1321 aatttgaaga cgtcgccatg caatattccg acggcgccaa tgccttgcag ggtggacgac
     1381 tcggattggt ccgacaaggt gaactcattc ccgccatcga acaggcggtc gcccacttag
     1441 tgccgggagg aatctcggac atcatcgaga gcccggaggg aatccagatt atccgcatgg
     1501 atgacagaaa gccgaaacag tttcgtcaat acgaggaagt gcggcgggaa attcaagaat
     1561 tggtctatca gcagaagagc gaagagatgt atcagtcttg gctggtggat ctaaagaata
     1621 aagcctacat tgagatcaag ttcgaacaag ttgccgataa agctcagctg cagcctctgc
     1681 attcatcacc ataagcaaga gttcaaggag catcggtgct aaggctgccg gccacgtcgc
     1741 gtaaggctct ccatacccga tcccgtccat ccccggtcac cgaggaataa ggaatcaaca
     1801 tttccccctc cgcgagtcct aaggccctat gcgtctctct tagagtacgt ac
//
LOCUS       DASTWE010000451         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_788088, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000451 DASTWE010000000
VERSION     DASTWE010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788088"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFT30_04765"
     CDS             complement(<1..964)
                     /locus_tag="VFT30_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091973.1"
                     /translation="MSRSTRALIVYMALLVSILLAIPVAAHPVSVSDTSRVDWFGKGR
                     AVANIGIISRNTTGAGEFVWTDAKSDQRIVSTDGATGNILSEIDLVKFNVTADTANLY
                     LLAKMDSITNVTNNPSPELMIAIDSDLNHTGGETALPDGIATNVAATAQWEYVVQTQF
                     TNVSATARPKVFTPTASGICLTCAAQLVSKDVQQGSFIELQIPWDQIGGLPAPENFLR
                     FTVLTHYSDKRAPSDGSSSTAIDVLSTTDTSDELSGDNTINTHFDLHFTSSGEVFAPL
                     LISEFLPDPPGSDTEGEWIEIVNPNSFPVSLQGYKVGDQVYRSGN"
BASE COUNT          292 a          333 c          373 g          291 t
ORIGIN      
        1 ggttgccact tcgatagacc tgatcgccga ccttataccc ttgcaagctc acggggaagc
       61 tgttggggtt gacgatctca atccactctc cctccgtatc gcttcctggc gggtcaggca
      121 gaaactcaga gatcaggagc ggcgcaaaga cctcaccact ggatgtgaag tgcaggtcga
      181 aatgggtgtt gatcgtgttg tcaccgctga gctcatcgct cgtgtcggtt gtgctcagca
      241 catcgatcgc tgtcgaagag ctgccatcgg aaggtgcccg tttatcgcta tagtgggtta
      301 gcacagtgaa acgtaggaag ttctccggcg cgggcaggcc gccgatctga tcccagggga
      361 tctgcaactc gatgaagctg ccctgttgga cgtccttact gaccaattgt gctgcgcagg
      421 tgagacagat tccactcgct gtaggcgtga agacttttgg acgagcggtc gcactcacgt
      481 tggtgaactg agtctgaacg acatattccc attgcgcggt agccgcgacg ttggtcgcaa
      541 tgccatcagg gagtgccgtt tctccacctg tatggttgag atcgctgtca atggcaatca
      601 taagctcggg cgatggatta ttggtgacgt tagtgatcga atccatctta gccaggaggt
      661 agaggttggc cgtgtcggcg gtgacgttga atttgacaag gtcaatttca gacagaatgt
      721 tgccagtggc gccatcggtc gagacgatcc gctgatcgga tttggcatcg gtccagacga
      781 actcacccgc accagtagta ttacggctaa tgatgccgat attagcaacg gcgcgccctt
      841 tcccgaacca atctacgcgg cttgtatcgc tcacgcttac tgggtgagcg gcgacaggga
      901 tggccaacaa gatgctgacg agtagtgcca tgtacacgat gagagcacgg gtcgatcgac
      961 tcatgggaat ctcctgaaca ataacactat gctgataaac agaagcaggt tcttgttgga
     1021 tagaacgggg tcggcggcca attagttacg aaatgagtat gaccagaatc cgtagcgccc
     1081 gaaagatcat tccactctgc cgaaggctat gtgtggctcg gagagctacg acgatacgtc
     1141 tgtgattgac aggctcgatc cacaccgcta caatctggca agcaaagcag cctttcggaa
     1201 ggcaaacaga cagtatgaca acgtttgatc tgcgcctcac tgccgctatc gccgctggca
     1261 aggctgccgg cctcgccagc cgtatgctg
//
LOCUS       DASTWE010000452         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_626326, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000452 DASTWE010000000
VERSION     DASTWE010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626326"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VFT30_04770"
     CDS             complement(<1..583)
                     /locus_tag="VFT30_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HEU5091974.1"
                     /translation="MATTQDTRERIIVPGPAGFHPPSAAQLGVSLPDPGEGLFYGLLE
                     PNEEVVIEEMARKMLTSPNATIFPGPLLLWAWNDHAVEKAKATLEIAAQIPEVMIIPM
                     PDYRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCCT
                     MAICAEQGHEDAMLTIRDSDTAKLKRVAQIFKRV"
     gene            complement(604..927)
                     /locus_tag="VFT30_04775"
     CDS             complement(604..927)
                     /locus_tag="VFT30_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEU5091975.1"
                     /translation="MYLVADISVEICAAKSCKLCTQYCPEANTIQYSDEMGKDKGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPKAALTENITYGEKSTTAVLA
                     SPVVG"
     gene            complement(948..>1546)
                     /locus_tag="VFT30_04780"
     CDS             complement(948..>1546)
                     /locus_tag="VFT30_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5091976.1"
                     /translation="YGSEKRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHG
                     KSYTMPFYFGLKEDGIALINNDGPMNLHRDQARELQERRAKLYYFPATKISLEVAGMD
                     LATNMALMGCIGAITGLTSMAGLDQAVKDRFLGKGFVVSGGTAALDSVVERKFKKKQE
                     LIEKNVAVMRAGWNYAVDHGWAAADVKRVEEPVAAATA"
BASE COUNT          313 a          451 c          384 g          398 t
ORIGIN      
        1 gtacccgctt aaaaatttgg gcaacacgtt tcaactttgc cgtatctgag tcgcggatgg
       61 tgagcatggc gtcttcatgg ccctgctcgg cgcaaatcgc catcgtgcag caattcgttc
      121 ccgcccggat cattttgagg gtcagattcg cataatgaca atggaccccg atgaagatac
      181 aggcttcgat cttattgccc cagattgtga gattggggtg attggggttg atgacttctt
      241 ccgggtcgat ctttggatac ttcggacgat agtccggcat cggaatgatc atgacttccg
      301 gaatctgcgc ggcaatttcc agcgttgcct tcgctttttc gactgcatga tcgttccagg
      361 cccaaagcaa cagcggtccg gggaagatgg tcgcattcgg gctcgtcagc attttacgag
      421 ccatctcttc aatcaccact tcctcgttcg gctcgagcaa tccgtaaaat aatccctccc
      481 ctggatccgg cagcgacaca cccaactgcg cggctgacgg aggatggaag cccgcggggc
      541 ccggtacgat gatgcgctct ctagtgtcct gtgttgttgc cacgcccttc ccctttcctc
      601 agtctatcca acgaccggac tggccaaaac ggccgtcgtg cttttttcgc cgtaagtgat
      661 attttcggtc agcgccgcct tgggcagctg atcgatcatg atcattttga tcgcattgtt
      721 ctttgccatg ttgtcgcaga cccacacaca ttgcgcacag cccttacagc gatccaccgc
      781 cacataggcc gaaccgtact tgaatccttt gtccttgccc atttcatcgc tgtattgaat
      841 ggtattggct tccgggcaat attgcgtaca gagcttacag cttttcgcgg cacagatttc
      901 aacgctgata tcggctacga ggtacatgaa gactcctttg tgaccgttta cgcggtggcc
      961 gctgccactg gctcttccac ccgtttgaca tcggccgcgg cccaaccatg atcgacggca
     1021 tagttccatc cagcccgcat gacggccacg tttttctcga ttagttcctg tttcttcttg
     1081 aatttccgct cgacaacgct gtcgagcgca gccgtaccgc cggacaccac aaagcctttt
     1141 ccgagaaacc gatccttcac ggcctgatcc aatcccgcca tactcgtcag gcctgtaatc
     1201 gcgccgatac aacccatcag cgccatgttg gtcgcgaggt ccatacccgc gacttccaat
     1261 gagatcttcg tcgctggaaa gtaatagagt ttcgcgcgac gttcttggag ctcacgcgcc
     1321 tgatctcggt ggagattcat cggcccgtcg ttgttgatca acgcaatgcc atcttctttc
     1381 aagccgaaat agaacggcat tgtataggac ttgccgtggg tgatgacctg tggatggaag
     1441 atgatgatga tgtgcggaaa cgtgatttca ccgatttcgt aaattggctc atccgacacc
     1501 cggacatagc tttcgaccgg cgccatccgc ttttccgacc cgtaga
//
LOCUS       DASTWE010000453         1384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1434586, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000453 DASTWE010000000
VERSION     DASTWE010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434586"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFT30_04785"
     CDS             <1..519
                     /locus_tag="VFT30_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEU5091977.1"
                     /translation="ANEGLPVVIVNPSAPVGEGDVKPTPTGQVIVDFMKGRMPAYIET
                     GMNIIDVDDVATGHLLAMEKGRQGERYILGSKNLLLREVFEILSKLTGLKAPALKLPR
                     GAVLPLAYLNHWFANFTGHPPRIPLEGVKMAKYKMHYDCSKAIRELGLPQHPPEVALE
                     KAVRWFRDHKYA"
     gene            590..>1384
                     /locus_tag="VFT30_04790"
     CDS             590..>1384
                     /locus_tag="VFT30_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEU5091978.1"
                     /translation="MEVFDLFIKTIILRPYVFIFLAAFLFSAIQLIGWPRTWRFWLIS
                     WATAFACEFSSTRNGIPFGWYFYNGSTVGQELYFSNIPFMDSISFSFLLFASYCVALG
                     LLLPFDSSPSIERVSLRPLQFDAKIRTSWTVFALTAFLFAFIDMVIDPVALRGDHWFL
                     GKIYYYPDPGWHFGVPFANYIGWAVVGLISLAIYFPLDRRLSPLSPPPPITERLLLGV
                     GLYYGVLAFNLGMTFWIGEFFMGMSGLLMHLPVLIILIARFSNVRRF"
BASE COUNT          257 a          389 c          360 g          378 t
ORIGIN      
        1 gccaatgagg gattgccggt tgtcatcgtg aatcccagtg cgccggtggg cgaaggcgac
       61 gtgaagccga cacccaccgg ccaagtcatt gtcgatttca tgaagggtcg gatgccggcc
      121 tatattgaaa ccggcatgaa catcatagat gtggatgatg tggccaccgg ccatctgctc
      181 gccatggaga aaggccgaca gggtgaacgg tatattctcg gctcaaaaaa tctgttgctt
      241 cgtgaagtgt tcgaaattct cagtaagctg acaggcctta aggctcccgc tctcaaattg
      301 ccacggggcg ccgtgttacc cctcgcctat ctcaatcatt ggttcgccaa cttcacgggg
      361 catccgccgc gcatcccgct ggaaggcgtg aagatggcca agtacaaaat gcactacgat
      421 tgcagcaagg cgatccgaga actcggctta ccccaacatc cccccgaagt ggcgctggag
      481 aaagccgtgc ggtggtttcg cgatcataag tacgcatgag gatgaattca ggatctggtt
      541 gtcaaccatg agtgcgtagc tcttctgaaa actcacaact caacgcttga tggaagtctt
      601 cgatctcttt atcaagacga tcattctccg cccctatgtg ttcatttttc ttgccgcgtt
      661 cctcttctcc gccatccaat tgataggctg gccgcgcacc tggcgcttct ggctgattag
      721 ctgggcgacg gcgtttgcct gtgagttttc gtcgacgcga aatggcatcc ccttcggctg
      781 gtacttctac aacggctcga cggtcggcca ggagctctat ttctccaaca ttccgttcat
      841 ggattctatt tccttcagtt tccttctctt cgcttcttac tgtgtggcac tcggactatt
      901 actgccattt gattcctctc cgagcatcga gcgcgtctct ctcaggccgt tgcaattcga
      961 cgcgaagatc cgcacgagtt ggaccgtctt tgcgctgact gcgtttcttt ttgcctttat
     1021 cgatatggtg atcgatccgg tggccctccg cggcgaccat tggtttctgg gcaagatcta
     1081 ctactatccc gacccgggct ggcattttgg cgtcccgttc gcgaactata taggctgggc
     1141 tgtggtcgga ctgatttcgc tggcgattta tttccccctg gaccggcggc tctctcccct
     1201 ctcgccgcct ccgccgatta cagagcggct gttgctcggt gtcggcctct actatggagt
     1261 cttggctttt aatctcggca tgaccttctg gatcggcgaa ttctttatgg gaatgagcgg
     1321 tttgctgatg cacctaccag ttttgattat cttaatcgct cgtttctcga atgttcggcg
     1381 gttc
//
LOCUS       DASTWE010000454         1386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1434598, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000454 DASTWE010000000
VERSION     DASTWE010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434598"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..>1386
                     /locus_tag="VFT30_04795"
     CDS             137..>1386
                     /locus_tag="VFT30_04795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU5091979.1"
                     /translation="MTFSLNMTVSDLLLLLPEIFLTCWLCVVLIVDFSLPRLPKERLA
                     YLSVAGLAVTLGCLGWFDLNHISGTLFGNMFVLDRMAIFFKVVILGATILVILASIEY
                     VNRFTLFRGEYYCLVVMAALGMMFMVSANDLLSVFVSLEFATLGFYVLVSYLRDDLAS
                     NEGGLKFFILGVFAAGLLAYGISLVYGETGKLVFSEMASAQATPGLVIGFLLIFASLG
                     FKIGAVPFHAWIPDTYHGAPTPVTTFLSIAPKAAAFAILLRIFLVALVTFKPLWVSLL
                     VAVSILSMTYGNIVAIAQRNIKRLLAYSGIAQVGTVLIGLAAGTKMGSDAILFYLLAY
                     LFANIGAFAVVIAVSQAIGRDEIEDYRGLNRRSPFLAFAMLLFLLSLAGVPPLAGFIG
                     KLYIFLAAIKEGLYTLIAVGLINIV"
BASE COUNT          250 a          358 c          371 g          407 t
ORIGIN      
        1 gcgtggttcc gatagcggaa accgatgagg ggttaggggt gaagggtaag atgacaacgc
       61 cagaaaaaag ttctgcgtcg ttggctgtgc acaaggccat atcctatacg cctcaggtct
      121 tacgagaggg gcgcgaatga cgttctctct gaacatgacc gtttccgacc tgctcctctt
      181 attgcctgag atctttctaa cctgctggct ttgtgtggtg cttatcgtcg atttttccct
      241 tcctcgcttg cccaaggagc gactggccta tctcagtgtc gcaggccttg cagtcacact
      301 cggctgtttg gggtggtttg acctcaatca tatttccggc acgctcttcg gcaacatgtt
      361 cgtgctggat cgcatggcga tctttttcaa agtcgttatt ctgggagcga cgatactcgt
      421 catcctcgca tcaattgagt acgtcaatcg cttcactctt ttcagggggg agtattattg
      481 tctcgtcgtc atggcggcac tcggcatgat gttcatggtg tccgccaacg atctgctgtc
      541 cgtcttcgtc agcctggagt tcgcaacgct ggggttctac gttctggtgt cttatctgcg
      601 tgatgatttg gcttcaaacg aaggaggact caagtttttt atcctgggtg tttttgcggc
      661 cggcctgctc gcctatggga tcagcctagt ctatggtgaa accggtaagc tcgtcttttc
      721 ggaaatggcg tccgcccagg caacacccgg cctggtcatc gggtttctgc tgatctttgc
      781 ctctcttgga tttaagatcg gcgccgttcc attccacgca tggattcccg atacctacca
      841 tggtgctccg actcctgtga cgactttttt gtcaatcgct ccaaaagctg cggcctttgc
      901 catcttgctc cgtattttcc tcgtggcgtt ggttacgttc aagccgctat gggtgtccct
      961 cttggtagct gtgtctatcc tgtcgatgac ctacggcaat atcgtggcca ttgcgcaacg
     1021 caacataaaa cggttactgg cgtattcggg catcgcccaa gtagggactg tattgatcgg
     1081 tcttgcggca ggcaccaaaa tgggaagtga tgccattttg ttttatcttc tggcgtacct
     1141 gtttgccaat atcggggctt tcgccgtcgt gatcgcggtc agccaggcca ttggaagaga
     1201 tgaaatcgag gactaccgag gcttaaacag acggtcgccg tttctggcat ttgcgatgtt
     1261 gctgttcctg ctgtctctgg ccggcgtgcc acctctcgcg gggtttatcg gtaaactcta
     1321 cattttcctg gcggctatca aagaagggct ttatacattg atcgcagtcg gtctgatcaa
     1381 catcgt
//
LOCUS       DASTWE010000455         1205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_400233, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000455 DASTWE010000000
VERSION     DASTWE010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400233"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..738)
                     /locus_tag="VFT30_04800"
     CDS             complement(142..738)
                     /locus_tag="VFT30_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5091980.1"
                     /translation="MRSDIVPGSVFPDYELPDHTARRRKLSDLQEADPMILVLSRGGY
                     CPKDRRQHEGLVQLHRELEVGYCRLVTISTDNITATNEYRRGVDAHWPFLSDDGRTVQ
                     KDLDIAEYTDSLHNPMIPHTIVLEPGLIVYKIYMGYWFFGRPTIEELRHDMRAVLMKC
                     RPDWDITKPEFKAAWSQERKEMFYPYGKTFLQTIREED"
     gene            complement(753..>1205)
                     /locus_tag="VFT30_04805"
     CDS             complement(753..>1205)
                     /locus_tag="VFT30_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091981.1"
                     /translation="HSLVLAVDNIDTARNDLVARGIEVSEAFHFAAGPFNDTVENPRV
                     SGRDPEGRSYFSFASFEDPDGNRWFLQEITSRLPGREWQTPSTDVATLADLLHETAEH
                     HDAYEKSHAKHDWWNWYAPYLSARQNGSSSEDAAAAADRYMEQLKPAS"
BASE COUNT          257 a          317 c          339 g          292 t
ORIGIN      
        1 gcaattctga tgattgcgtt gactgagctt tgaccaaact catcagtggc aactgtgttg
       61 ccgcaagggt catagccgta ctggcaataa accgccgccg gttctgatcg atttcttcct
      121 gtttgaaatt catctcggtg atcaatcttc ttctcgtata gtctgaagaa atgtcttgcc
      181 atacggataa aacatctctt tacgttcctg ggaccacgcc gctttgaatt cgggctttgt
      241 gatgtcccaa tcgggtctgc atttcatgag gactgcacgc atatcatggc gaagttcttc
      301 gatagtcggc cgtccgaaaa accagtaacc catgtagatc ttgtaaacga tgaggccggg
      361 ctccaggacg atggtgtgag ggatcatcgg attgtgtagc gaatcggtgt actcggcaat
      421 gtcgagatct ttttgaacgg tgcgaccatc gtccgagaga aagggccagt gagcatcaac
      481 gcctctgcga tactcgttcg tcgcggtgat gttgtcggta ctaatcgtta ccaaccggca
      541 atagccaacc tcaagctcgc gatgaagttg aacaagtcct tcatgctgcc ggcgatcttt
      601 tggacagtaa ccaccgcgac tgagtacgag tatcatcgga tccgcttctt gtaggtcgga
      661 gagcttccgg cgtctcgcag tgtggtcggg aagctcatag tctgggaaga cagaccctgg
      721 aacgatgtca gatcgcattt gcaacacccc ttttaactag ccggcttgag ctgttccatg
      781 tagcgatcgg ccgcggcggc ggcgtcttcc gaactgctgc cgttctgccg cgcgctcaga
      841 tatggcgcgt accaattcca ccagtcatgc ttggcatgcg acttttcgta ggcgtcgtgg
      901 tgctccgctg tctcgtgtag aagatccgca agcgtggcga cgtcagtgga cggtgtttgc
      961 cactcacggc cgggtagccg cgatgtgatc tcctgaagga accaccggtt cccatcggga
     1021 tcttcgaacg aggcaaacga gaagtaggaa cgaccttccg ggtcgcgccc cgagacgcgt
     1081 gggttctcta cagtatcgtt gaaaggacca gcggcgaaat ggaaggcttc actcacctcg
     1141 ataccgcgag cgaccaagtc gttgcgcgca gtgtcgatgt tatcgaccgc gaggaccagg
     1201 ctgtg
//
LOCUS       DASTWE010000456         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1305323, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000456 DASTWE010000000
VERSION     DASTWE010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305323"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..678
                     /locus_tag="VFT30_04810"
     CDS             244..678
                     /locus_tag="VFT30_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5091982.1"
                     /translation="MSEFKSGFFVGGESTNGRVLIVDDEPDIRKVVRMTLQKAGYDVL
                     EAENGEKAIEVINSGENRLLLDVIICDIRMPKVNGIEAIAYFRQNYPRVPLIVLTGFP
                     DTDMATSLLRQGVVDYLVKPVEGEKLKASVARAMEQRELAHL"
     gene            708..1811
                     /locus_tag="VFT30_04815"
     CDS             708..1811
                     /locus_tag="VFT30_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5091983.1"
                     /translation="MAATSSPNVHPGVEIEQASTPRPMRVAIIGAGRGGTALLDVLHQ
                     IGTIEVVGITDKDPLAPGLQRAREINVTIYDQVADLIKSPGLNLVMDVTGDLAMGPVL
                     QEQLPTEVDIISGQASRLLWTLVQHESTLQTELLHAEKLAGIGSFAAGIAHDMNNPLQ
                     LILGLAENLSDEEDLDAVHAQARDIVDAVKRTTAICRDLTSYSRRTSLRQDCMVHVNG
                     KLDEALKIARYAVALQDIDIRKLYQPDVVIKGNPDELLHVFVNLITNAVHAMEHHGTL
                     TLETSAVAGTIQVRVSDTGCGIEPELQSRIFEPFFTTKPPGKGTGLGLYNIKNVIQRM
                     NGSIGVDSHIGRGSTFTLTFHDEPAAGGRSTQS"
     gene            1808..>2143
                     /locus_tag="VFT30_04820"
     CDS             1808..>2143
                     /locus_tag="VFT30_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091984.1"
                     /translation="MTETPVSSPPRGNWGLKAKIIVSMLLVGLIPLIVGLGMAFWQGS
                     QEIREVSGESFEALATEAARKLDLLLAEEIAHTARIANDPMLIRELEHRRDARGGVKS
                     ATTAPSDLER"
BASE COUNT          511 a          608 c          582 g          442 t
ORIGIN      
        1 ttccccctca cctttttcaa taccctagcc gaaattagct ctgaaacgca atgagcatcc
       61 gcgttggtgg tggtggtttt cagcattgtg gtggtgcaac tgagccacta atgggggaaa
      121 gccagctggc ttagcacata agcagttgaa attattttaa tgccgctcca tcacaattcg
      181 gcatcgactt tgcgcctatc gttgtgcacg caaacatttt ccctcttcac caaggaggtc
      241 atcatgagtg aattcaagtc cggatttttt gtgggaggag aaagcaccaa tggtcgagtg
      301 ctgatcgtcg atgacgagcc cgacatccgc aaggtcgtcc ggatgacttt gcagaaagcc
      361 ggctacgatg tcctcgaggc ggagaatgga gagaaagcca ttgaagtcat caattcgggt
      421 gaaaatcggc tgctccttga cgtcatcatc tgcgacatcc ggatgccgaa agtgaacggc
      481 atcgaagcga ttgcgtactt tcgccaaaac tatccgcgcg tgccgctgat cgtcctgacc
      541 ggattccccg acacagacat ggccacgtcg ttactgaggc agggcgtggt ggactatctc
      601 gtaaagcccg tggaaggcga gaaattgaaa gcctctgtcg ctcgtgccat ggaacaacgg
      661 gaattggccc atctctgatg gactggatga catcacaggg aaagagcatg gcggccacct
      721 cttcacccaa cgtccatcca ggggtcgaaa tcgagcaggc atccaccccg cgtccaatga
      781 gagtggccat tatcggcgca gggcgggggg gcaccgcact gctcgatgta ctccatcaga
      841 tcggcacgat tgaggtcgtc ggcattaccg acaaggatcc cttagcacca ggactccaac
      901 gggctcggga aatcaacgtg acgatctatg atcaagtcgc cgacctgatc aagagcccgg
      961 ggctcaatct tgtgatggac gtcacaggtg atttggcgat ggggccagtg ctccaggaac
     1021 aactgccgac agaagtcgac atcatcagtg gacaggcgtc gcgacttctc tggacactcg
     1081 tgcagcatga atcgacattg caaaccgagt tgctccacgc ggagaaactc gctggaatcg
     1141 gttcgtttgc ggcgggcatc gcccacgata tgaacaatcc ccttcagctg attctcggtt
     1201 tggcggagaa tctgtccgat gaagaagacc tcgatgccgt gcatgctcaa gcacgggaca
     1261 tcgtcgatgc cgtcaagcgc acgacagcca tttgtcgaga tctcacctca tactcacgcc
     1321 gcacatcctt acggcaagac tgcatggtcc acgtcaacgg caagttggat gaagccctta
     1381 agatcgcccg ctatgccgtg gcgcttcaag acattgacat tcgcaagctc tatcaacctg
     1441 atgtcgtcat aaagggaaac cccgatgagc tcctccatgt gttcgtcaat ctcattacga
     1501 atgccgtcca cgccatggaa caccacggca ccttgacgct ggaaacctca gccgtggccg
     1561 gcacaataca ggttcgtgtt tcggacaccg gctgcggcat tgagcccgag ctgcaaagtc
     1621 ggattttcga accttttttc accacgaagc caccagggaa aggaacgggg ttgggcttat
     1681 acaacatcaa aaatgtcatc cagcgcatga atggctcaat cggggtcgac agccacatcg
     1741 gccgtggctc aaccttcacc ctcacctttc acgatgaacc cgccgctggg gggaggagca
     1801 ctcagtcatg acggagacac cggtatcatc gcctccccgc ggcaattggg gactcaaggc
     1861 aaagatcatt gtctccatgc tcctcgtcgg tctcatcccg ctcatcgtcg gtttagggat
     1921 ggcattctgg caaggatctc aagaaatccg ggaagtcagc ggtgaaagct tcgaggcgct
     1981 ggcgactgaa gccgcccgca aactcgatct cctgctcgct gaagaaatcg cccacacggc
     2041 gcgcatcgcg aatgatccga tgctcatccg agaactggaa catcggcgtg atgcgcgggg
     2101 tggcgtcaaa agtgccacca cggcaccctc ggacttagaa cgg
//
LOCUS       DASTWE010000457         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_885303, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000457 DASTWE010000000
VERSION     DASTWE010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885303"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>1015
                     /gene="pyrG"
                     /locus_tag="VFT30_04825"
     CDS             22..>1015
                     /gene="pyrG"
                     /locus_tag="VFT30_04825"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HEU5091985.1"
                     /translation="MRISKGIDVTIVEIGGTVGDIESLPFLEAIRQMPYDVGRDNVLY
                     VHLTLVPYIGTAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYIPPELKGKIAMFCN
                     VDKDAVITAKDVETIYEVPIVFRKEGLDELIVRQLHVETGQPNLREWDAMVQKIKRPK
                     HEISVALVGKYVGLKECYKSLGEALVHGGIDHETKVNINWIESEDIERQGTERILREA
                     DGILIPGGFGTRGIEGKVTTIRYARERQVPFLGLCLGMQCAAIEFARNVAGLAGANSA
                     EFDERSPHPVIHLMPDQHSVSNKGGTMRLGSYLCKLGEGTLAQKMYGVSEVRERH"
BASE COUNT          258 a          235 c          312 g          210 t
ORIGIN      
        1 gatgagatca agcaggcgat catgcggatt tcaaaaggaa tcgatgtcac gatcgtggag
       61 atcggcggga cggtcggcga cattgagagc ttgccgtttc tcgaggccat ccggcagatg
      121 ccgtacgacg tcgggcgtga caatgtgttg tacgtccacc tgactttagt gccctacata
      181 ggcacggccg gtgaattaaa gactaagcca acgcaacatt cggtgaacaa acttcgagaa
      241 atcggtatcc agcccaatat tctattgtgc cgaaccgacc gctatattcc cccggagctc
      301 aagggcaaga tcgcgatgtt ctgcaatgtg gataaagatg ccgtcatcac agccaaagat
      361 gttgagacga tctatgaagt accgatcgta ttcagaaaag agggactgga cgaattgatc
      421 gttcgtcagc tccatgtgga gaccggccag cccaatcttc gcgagtggga tgcgatggta
      481 cagaagatca agcgtccgaa gcatgagatt tctgtcgcgt tggtgggcaa atatgtgggt
      541 ttaaaagaat gctataagag tctgggagag gcgctcgttc acggtgggat cgatcatgaa
      601 acgaaggtca atatcaattg gatcgagtcc gaagatatcg agcggcaagg gacggagaga
      661 atcctgcgag aggctgacgg catcttgatt cccggtggat tcgggacaag aggaatcgag
      721 ggaaaagtga cgaccattcg gtacgcgcgg gaacgtcagg tgccattcct cggcctgtgt
      781 ttagggatgc aatgtgccgc aatcgagttt gcgcggaatg ttgccggatt ggccggcgcc
      841 aacagcgccg agtttgacga gcggtcgccc catcccgtga tccatctcat gcccgatcag
      901 catagcgtga gtaacaaggg tggaaccatg cgactcggat catacctctg caagttgggg
      961 gaggggacgc ttgcgcagaa aatgtacggc gtgagcgagg tccgtgaacg ccatc
//
LOCUS       DASTWE010000458         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1337690, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000458 DASTWE010000000
VERSION     DASTWE010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337690"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..529
                     /locus_tag="VFT30_04830"
     CDS             209..529
                     /locus_tag="VFT30_04830"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5091986.1"
                     /translation="MTRESRKVERFAVQFPCKFWNKTDKSDGTVLNLSAQGCVITAAH
                     LPSVFTYVSLDIDLLTDEGLVTIELAGVRWVSERRCGLEFIRIRPDMLMKLRAFAWLL
                     EQTS"
     gene            complement(561..2387)
                     /locus_tag="VFT30_04835"
     CDS             complement(561..2387)
                     /locus_tag="VFT30_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU5091987.1"
                     /translation="MIAQDVETHHKRQILSVASLKKSSMAKQPRKMTMGRSLLDCIVY
                     RGLIKQADLDAAIEESLSREIDLESLLLDKYRVPRSALGSALSEFYQCPYVSYDERTV
                     IDPELLKNLSFDYLRKSAWIPLKRQGTVLDIVINDPHDLEKGLDIRRAFPGTTIRFSV
                     GLRRDIEQYLLVATGQANGGSITEILGELVDEAGIERNGEAESGEINENDSAIVRLAN
                     HVIAEAYRLGASDVHIEPYSDRKETAVRFRVDGTCFTYMRIPAVYRRAIVSRLKIMAN
                     LDIAERRKPQDGKIRYKLAKDHEIELRVATLPTAGNNEDVVLRLLTAKDTMPLEAMDF
                     SQDVLQIVKELSERPHGIFLCVGPTGSGKTTTLHAIMKHINTDERKIWTAEDPIEITQ
                     EGLRQVQVQPKIGFTFATAMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVMST
                     LHTNSAVETVTRLLDMGCDSFNFADAMLGVLAMRLCKRICSHCKEAYHPTQQEYDELV
                     QGYGARYWEKLGVTYTEDWTLYRGRGCEICNHSGFKGRVALHELLVGSEEIKNLIQAR
                     ARTAEILSVAMRDGMVTLLQNGIQKILNGLTTYRQVRAVAIK"
     gene            complement(2468..3937)
                     /locus_tag="VFT30_04840"
     CDS             complement(2468..3937)
                     /locus_tag="VFT30_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HEU5091988.1"
                     /translation="MSHVHVKDGQQGIALLGAMVVALILSLLGATLLNLAGQETVSAG
                     LASQAAVAQQLADAAGELVVTWFHSPQTIAGVAQLSSLQEKRNRDKEGAPSFFDAYGR
                     SQFVGTADQPDVRLQIGNPSDNGLLNDPETGMFRTMTHLGQVEELKVYAPSNPGLLCT
                     IDTTIVTNTNPPVRQSILMQLGGLDVPPLRAAVQVGQHLGRFQSGNESPVSVHWGELK
                     VGGDLILTQADEIPLRTALAPVTGQAYEEMTQREDRWMEGWIGGTVQVTHLPSEQIPS
                     FPSNLHMGQNPIPGVRLDQWPYEQIKRVAKRFGRYFALDREGLLYPQGDVEPGRGISP
                     DEVFRSQGSGDQLGLIFIDTLDQTPPRADNLGVVRLQAPYFEGMAVVQGHVVLAPSGS
                     GQSIGVRSPQLIDQGKDFGRTPVQLSGMHFNGVLHASGDITIVGRVRLYGAVAAGGTI
                     TSAGSGSSLEVWYDHDLSRGLYRGLPQVYRAPGTWMTRY"
     gene            complement(3949..>4273)
                     /locus_tag="VFT30_04845"
     CDS             complement(3949..>4273)
                     /locus_tag="VFT30_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091989.1"
                     /translation="RLFRSGQQYQLNLSEPVTLSFPAGASVEVSNRVVYYTKQDENGT
                     VKLMRMVDGGANILIGDLEDLHFSYWDEHGHVTQQPSLVKRVVLEIDSHHSLHRMMRE
                     VSLRS"
BASE COUNT          962 a         1162 c         1049 g         1100 t
ORIGIN      
        1 gcattgtttc tcgtcgaagc ccaataaaat tcgttcactt ttcgcactca tacacgacaa
       61 tttttggctg aagtgttgca ctgtttggta cagcaaagag gaccttttat tgacagaggg
      121 catggtcgcc attaggtaat aagtttgcag gctatgagcg aagtgaagat gtgtcgagtt
      181 caaccgtaca ttggcctaga gaggtacgat gacaagagaa tctcgcaagg tcgaacggtt
      241 tgctgttcag tttccctgca aattttggaa caagacagac aagtcggacg gaacggtcct
      301 aaacctttcg gcacaagggt gcgtcataac ggcggcgcac cttccgtccg tctttacata
      361 tgtttcactg gatatcgatc tcttaaccga cgaagggctg gttacgatag aattggccgg
      421 agttcgttgg gtttccgaac gccggtgtgg gctggagttc atccgcattc gacccgacat
      481 gctcatgaag ctgagagcct tcgcttggtt gctggagcag acctcttaat agattgaact
      541 ggttgtttgc tccagcgatg ctactttatc gcaacggctc ggacttgccg ataggtcgtc
      601 agaccattca gtattttctg gataccattc tgcagcaggg taaccattcc atctcgcatg
      661 gccacgctta gaatttcggc tgtcctcgct ctggcttgga tcaaattttt aatttcttct
      721 gaacccacga ggagttcgtg tagtgccacc cgccctttaa atccgctgtg attgcagatc
      781 tcacacccac gtccgcgata aagggtccag tcctccgtgt aggttacccc tagcttttcc
      841 cagtatcgcg ctccatagcc ttgcacgagt tcgtcatact cttgctgagt cgggtggtag
      901 gcttctttgc aatgagagca aatgcgcttg cagaggcgca ttgccagcac ccccaacatc
      961 gcgtcggcaa agttgaacga gtcgcaaccc atgtccagca atcgagtgac tgtttctacg
     1021 gcactgttgg tatggagcgt gctcatcacc agatgaccgg taagagaagc ttctatcgcg
     1081 atatcggccg tttctttgtc tcgcatctcc ccgatcatga tcacgtccgg gtcggcccga
     1141 aggaatgcgc gcatcgctgt ggcgaaggta aatccgattt taggttgcac ctgaacctgc
     1201 ctcaaacctt cttgagtgat ttcgatggga tcctccgcgg tccaaatctt tcgttcatcg
     1261 gtgttgatgt gtttcattat ggcgtggagc gtcgtggttt tccctgaccc agtcggcccc
     1321 acacaaagga aaattccatg cggacgctcg gacaattctt taacgatctg cagaacgtct
     1381 tgggaaaagt ccatggcctc taagggcata gtatcctttg ccgtgagaag ccgcagcacc
     1441 acatcctcat tatttccggc ggtaggcaat gtagctaccc gcaattcgat ttcatgatcc
     1501 ttcgctaatt tgtagcggat ttttccatcc tgaggcttcc gtcgctcggc gatatcgagg
     1561 ttggccataa tcttcaaccg cgaaactatg gcacgccgat aaacggcagg aatacgcata
     1621 taggtaaaac aggtcccatc gacacggaac cgtactgccg tctccttgcg atccgaatag
     1681 ggttcaatat ggacatctga tgcccctaat cgatatgcct ctgcgatcac atggttcgcg
     1741 aggcgcacaa tggctgaatc attttcattg atctctccgg actcggcctc gccgtttcgt
     1801 tcgatgcccg cttcatcgac gagttctccg agaatctcgg ttatcgatcc cccgttggct
     1861 tgccctgttg cgacaaggag atattgctcg atatcccgcc gaagcccgac ggagaaacgt
     1921 atcgtcgtac cagggaacgc acgtcgtata tcgaggcctt tctccagatc gtgtggatca
     1981 ttgatcacaa tgtcgaggac agtcccctgt cgtttcaacg gaatccaggc gctctttctg
     2041 agataatcga aactgagatt tttcaaaagc tccgggtcga tgacggttcg ttcatcatag
     2101 gaaacgtagg ggcattggta aaattcgctg agcgccgacc ccagtgcaga cctcggcacg
     2161 cgatacttat cgagcaagag gctttctaga tctatttccc gtgatagaga ttcttctatt
     2221 gcagcatcga gatccgcctg tttgatcagc ccgcgataga cgatgcagtc taagagactg
     2281 cgtcccatcg tcatcttgcg aggttgtttg gccatcgacg acttttttag actcgctacc
     2341 gagagaatct gccgtttgtg atgcgtttca acgtcttgcg cgatcatcct agctcatcct
     2401 ttctcaccga ccataagaag ctgtttgtat atgtcagcct tctttgacgt tcgatctcac
     2461 cttcgactca atatcgtgtc atccaggtac caggggcacg atagacctga ggcaatccac
     2521 gatataaccc acgactgaga tcgtggtcat accacacttc caaactgctt cctgaaccag
     2581 ctgaggtgat tgtcccacct gctgccactg ctccgtatag tctgactctt ccgacgatcg
     2641 tgatatctcc acttgcgtgg agtactccgt tgaaatgcat gccggagagt tgaacgggtg
     2701 tccgaccgaa atccttaccc tggtctatca gttgaggact acgtaccccg atagactgtc
     2761 ctgatccgct gggagcaagc acaacatgac cttgcactac cgccatgcct tcaaagtacg
     2821 gagcctgcaa tctgacaacc ccgagattgt ctgcacgggg aggcgtctga tcgagcgtgt
     2881 cgatgaatat cagaccaagc tggtcgccgc ttccctgtga ccgaaatacc tcgtctggag
     2941 aaatgccacg tcctggttct acatcacctt gcggatagag aagaccttct cgatcaagcg
     3001 cgaagtaacg gccgaatctt tttgccactc gctttatctg ttcatagggc cactgatcga
     3061 ggcgaactcc cggaatcgga ttctggccca tgtgaaggtt gctggggaaa ctaggaatct
     3121 gttcggatgg taaatgtgtc acctgaaccg tcccgccaat ccatccttcc atccatcgat
     3181 cttctcgttg cgtcatttcc tcataggctt gtcctgttac cggagcaagc gcagtcctca
     3241 atgggatctc atcagcctgt gtgaggatca gatcacctcc cacttttaac tcaccccaat
     3301 gcacagagac cggtgattcg tttcctgact ggaagcgacc gagatgctgg ccaacttgca
     3361 cagcggctcg taacggaggt acgtccagcc cgcctaactg catgagtatc gattgcctga
     3421 ccggaggatt agtattggtt acaatcgtgg tgtcgattgt gcacaggaga ccaggattgg
     3481 aaggcgcgta gactttgagt tcttccacct gtcctagatg tgtcatagtg cgaaacatcc
     3541 cggtctcagg atcattcaac agtccgttat ccgagggatt gccaatctgt aaccgtacat
     3601 ccggctgatc cgccgtacct acgaattgcg agcgaccgta cgcatcaaaa aaggatggcg
     3661 ctccttcttt atcgcggttt cttttttctt gaagggatga aagctgtgcc acgcctgcaa
     3721 ttgtctgggg gctgtgaaac caggtcacca ccaattcacc agccgcgtcg gccaattgct
     3781 gagcaaccgc ggcctggcta gctaatccag cacttacggt ctcttgtcct gcgagattga
     3841 gaagcgtcgc gcccaacagc gataggatga gggcgacaac catagctcct agaagtgcaa
     3901 tcccctgctg gccatctttg acatgtacat gcgacatgtt tctgctcctt atgatcgtag
     3961 actcacttcc cgcatcattc tatgcagaga gtgatgcgag tcgatttcga gcacaactcg
     4021 cttcacaagc gagggctgct gcgtgacatg gccatgctcg tcccaatacg aaaaatgtag
     4081 atcttccaag tcgccgatga gaatattcgc tcctccatcg accatccgca taagcttcac
     4141 cgttccgttt tcgtcttgct tggtgtaata gaccacccga ttgctcactt ccaccgaggc
     4201 acccgcggga aacgacaagg tcaccggttc actcaagttc agctgatatt gctgccctga
     4261 acggaaaagt cga
//
LOCUS       DASTWE010000459         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1466924, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000459 DASTWE010000000
VERSION     DASTWE010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466924"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..>1598
                     /locus_tag="VFT30_04850"
     CDS             3..>1598
                     /locus_tag="VFT30_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5091990.1"
                     /translation="MRTNIDIPDLIEIQKRSYEEFLQFEVESERRKDHGLQAALASVF
                     PIPDYNNTAVLEFSSYTLGTPKYDERECLEQGMTFAVPLKLRVRLVVLDKEDKGPRKK
                     VLDVREQEVYVGELPLMTERGTFIVNGTERVVVSQLHRSPGASFTHDKGRTHASGKVL
                     YSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKAFGFSSDDLLKMYYPVEE
                     IRVSKGKMFRKLDPEIHHGLRCSAEVTDKGGKEPLVREGTRLTKGLIAKLKASGVKEI
                     PLQPAELVGRAILTELVDSKKNKLAEKNQRLTAEMVEQIAESDVEEFKVIYLDMATAT
                     PVILDTLEMEKIGSKEEAMVEIYRRLRPGETPSVDTARALFDNLFLNSKRYDLSPVGR
                     LKLNKKLGLDLPLEQRTLTAQDIVEVIRYLVNLKIGKGEIDDIDHLGNRRVRSVGELL
                     ENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVAAVKEFFSSSQLSQFMDQT
                     NPLAEITHKRRLSALGPGGLTRER"
BASE COUNT          376 a          373 c          487 g          362 t
ORIGIN      
        1 gcattcgaac caacatcgat attccggatc tgattgagat ccagaagcgc tcttacgaag
       61 agtttctgca gtttgaggtc gaatcggagc gtcggaagga tcatggattg caggcggcgt
      121 tggccagcgt gtttccgatt cccgattata acaacacggc cgtgctcgaa ttttcaagtt
      181 acaccttagg gacgccgaag tacgacgagc gcgaatgtct tgagcagggt atgaccttcg
      241 cggttccgtt gaagctgcgt gtccgcctgg tggtgctcga taaggaagat aaggggcctc
      301 gcaagaaagt gctcgatgtg cgtgagcagg aagtctatgt cggtgaattg ccgctcatga
      361 ccgagcgagg gacttttatc gtcaacggga ccgagcgagt cgttgtcagt cagcttcacc
      421 ggtcgccggg tgcgtcgttt acgcatgaca agggtcgaac ccatgcgagc ggcaaggtgt
      481 tgtactcagc ccgaattatt ccctatcggg gctcatggct cgatttcgag ttcgatgcca
      541 gggatattct ctatgtgcgg atcgatcgtc gtcggaaaat gccggccacc attctgttga
      601 aagccttcgg tttctcgagc gacgatttgc tgaagatgta ctaccctgtc gaggaaattc
      661 gcgtgtcgaa ggggaagatg ttccgcaagc tggatccgga aatccaccac ggacttcggt
      721 gttccgccga ggtgacggac aaaggcggca aagaaccgtt ggtgcgagaa ggaaccaggt
      781 tgacgaaagg tctgattgcc aagctgaagg cctcgggagt gaaggaaatc cctttacagc
      841 ctgcagagtt ggtagggcgt gccattctca cggaattggt cgactcgaag aagaacaagt
      901 tggcggagaa gaatcagcga ttgacggccg agatggtcga gcagatcgcg gaaagcgatg
      961 tagaagaatt caaggttatt tatctggata tggcgaccgc gacgccagtg attcttgata
     1021 ccttggagat ggagaaaatc gggtcgaagg aggaagcgat ggtcgaaatc taccgtcgtc
     1081 tccgccccgg tgagacgccg tcagttgata cggcgagagc attgttcgac aatctctttc
     1141 tgaattccaa gcgttacgat ttgtctccgg tcggccggct caagctgaat aagaagctgg
     1201 gtttggactt gccgctcgag cagcgcaccc tgaccgctca ggacattgtg gaagtcatcc
     1261 gctacctcgt caatctgaag atcggcaaag gggaaatcga cgatattgat cacttgggta
     1321 atcgccgcgt gcgatccgtt ggcgagcttt tggaaaatca atttcggctg ggtttggtgc
     1381 ggatggagcg aagcatcaag gaacgcatga atcttcttga tatggaaacg gtgctgccgc
     1441 atgacttgat caatgccaaa ccggttgtgg cggccgtcaa agagtttttc agcagcagcc
     1501 agctgtctca attcatggac caaacgaatc cactggctga aatcacgcat aaacggcgtc
     1561 tgtcggctct tggtccaggc gggctcacga gggagcgg
//
LOCUS       DASTWE010000460         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_659170, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000460 DASTWE010000000
VERSION     DASTWE010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659170"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFT30_04855"
     CDS             complement(<1..228)
                     /locus_tag="VFT30_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEU5091991.1"
                     /translation="MSDRTVPEQDEPNDRSEETSPKTGTDPLELIEQCLASLPEGDAR
                     QKLLYKLRHAVTLSQAAHQQRELEFKKVSEVV"
     gene            complement(298..1374)
                     /locus_tag="VFT30_04860"
     CDS             complement(298..1374)
                     /locus_tag="VFT30_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5091992.1"
                     /translation="MLLCSPINAAEWGKPLGAVYDGPEGKPRPVTPSPVELSADERST
                     IAVFERATKSVVFIANTAIQQDIWSLNVMEVPQGSGSGFVWSKQGHIVTNFHVIYGAD
                     AIKVTLADRSEHKAKLVGADPDHDLAVLQIQVPDGHVEPLAIGSSHDLRVGQKVLAIG
                     NPFGLDHTLTTGVVSALGRTIKSMSNRTIEGVIQTDAAINPGNSGGPLLDGAGRLIGV
                     NTQIVSPSGAYAGIGFAVPVDTVNRIVPELIKHGKLIRPGLGVSLVPDAMAKRWGIKG
                     VIIGKVTHGGAADRAGLKGARETMIGQIQLGDVIVSVAGKPVATIDDLMDVMEAHKVG
                     DQVSVEIVRGNRREKVSVTLQAVN"
     gene            complement(1412..1837)
                     /locus_tag="VFT30_04865"
     CDS             complement(1412..1837)
                     /locus_tag="VFT30_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU5091993.1"
                     /translation="MMSVHTVSLTIRMEGDTQVDNITNSVADALAGSNLKAGVVTVFV
                     KHTTASVMIIEDEPGIRADTKTFWNRLVPPDHRWQHNTVNPGEDNGHSHLRGQLQGPS
                     VTIPFSAGALLLGTWQQIVMVDFDTRPRTRELVVQVLGE"
     gene            complement(1834..2649)
                     /locus_tag="VFT30_04870"
     CDS             complement(1834..2649)
                     /locus_tag="VFT30_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091994.1"
                     /translation="MKLYDYPHPRRPGRTIRGYDRPHAVRTAKMCVTVADRLGHPGDR
                     VRQYHVACLLHDLGRAGLDRQLFGTIWSWAKQRGIPTRPREWRALHPRTTYGRETEAF
                     VSLYRQDLIASGVPMDTWAVEQIEMRLGYARRLARRLRMVKPKLKTLGVEWQPWMQQV
                     MLYYYYPERLAKAKAWVRQLAEVLVACEQFEAYSNQRRGRDYYARNKESLSEAFAYLD
                     KLGQEGILSSQVLSAVRSLTAEGVFDSILEEARGEPLTRSDRRYLRSLAGRRL"
     gene            2867..>3409
                     /locus_tag="VFT30_04875"
     CDS             2867..>3409
                     /locus_tag="VFT30_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091995.1"
                     /translation="MTNGLRICCLVAASAIVTEPVCSFAGSLDQLTDLTGRVQAIVTL
                     KSRDNFTSEYRYDVSVRNLSSDAIIGDSVVIVLDKITNLAGEDREGLTGESFLKRFDV
                     LDQDGQTEDGKPYFRVPVGTSPDLAPQTDSLPAVVRIRNRDYVAVFTPSFKVLGQKRP
                     PPEAKRAESPISSTPPAPGTS"
BASE COUNT          735 a         1024 c          917 g          733 t
ORIGIN      
        1 gacgacctcg ctcactttct tgaattccag ttcacgttgt tgatgggcgg cttgagacaa
       61 tgtcaccgca tgacgcagct tgtacagcag cttctgtcga gcatccccct cgggaagtga
      121 ggccagacat tgctcaatca attcgagcgg atcggtcccg gtcttcggcg acgtctcttc
      181 gctccgatcg ttgggttcat cctgttcagg aactgtgcga tcactcataa gtggtccttt
      241 acgacgcttg accaaatgat cctaacacac acatgtcttg caatgccttc acgctggcta
      301 gttgacggcc tgaagagtta ccgacacctt ttcacgccga tttcctcgca caatctccac
      361 gctcacttga tcaccgactt tgtgcgcttc catcacatcc atcaggtcat caatggtggc
      421 caccggcttt ccggcgaccg agacaatgac gtcgccgagt tggatctgcc cgatcatggt
      481 ttcacgcgcc cccttgaggc ccgcgcgatc ggcggcgccg ccgtgagtta ccttaccgat
      541 gataactccc ttgatccccc atcgctttgc catcgcgtcc ggtacgagag agactcccag
      601 ccctggacgg atgagcttgc cgtgcttgat gagttccgga acgatacggt tcactgtatc
      661 gaccggaacg gcaaaaccaa ttcctgcata agctccgctc ggactcacga tttgcgtatt
      721 cacgccgatc aatcgcccgg ccccatctag aagcgggccg cccgaattac cgggattgat
      781 cgccgcatcg gtctggatca cgccttcaat cgtccgattc gacatggatt tgatggtccg
      841 ccccaatgca ctcacgaccc cggtcgtcaa ggtatgatcg agcccgaacg gattcccgat
      901 cgccaacacc ttttgcccta cccgcaaatc atgcgaggaa ccgatcgcca acggctccac
      961 atgaccatcc ggcacctgaa tttgcaacac cgccagatcg tgatcgggat cggcgccgac
     1021 cagcttcgcc ttatgttcgc tccgatcggc caacgtgacc ttgatcgcat cggcaccgta
     1081 aatgacatga aagttggtga cgatgtggcc ttgcttgctc cacacaaaac cggaccctga
     1141 cccttgaggc acttccatga cgttgagcga ccaaatatct tgttggatcg ctgtgttggc
     1201 gatgaacacg acagacttgg tcgcacgttc aaacactgcg atcgtcgatc gttcatcggc
     1261 actcagttcc accggcgagg gcgtcaccgg gcgtggcttc ccttccgggc cgtcatagac
     1321 ggcacccagc ggcttgcccc actccgccgc attgatcggt gagcacagca gcattgacgt
     1381 aaccaggaac aatgtcaggg atccggcgcg gctattcacc cagaacctgc acgacgagtt
     1441 cacgcgttct ggggcgggta tcgaaatcca ccatcacgat ttgctgccac gtgccgagca
     1501 gaagtgcgcc ggccgaaaac ggaatggtga cggacggccc ttggagttgt cctcgaagat
     1561 ggctgtggcc gttgtcctcg ccgggattca ccgtgttgtg ctgccagcgg tgatcaggag
     1621 ggacgagacg attccaaaac gtttttgtgt cggcccgaat tcccggttcg tcctcgataa
     1681 tcatgacgga ggcggtggtg tgcttgacaa ataccgtcac gactccagct ttgagattgg
     1741 aaccagccaa ggcatcggca acagagttcg tgatgttgtc gacctgcgta tcaccctcca
     1801 tacggatcgt cagcgagacg gtgtggacac tcatcatagt ctccgacccg cgaggctgcg
     1861 aaggtagcga cggtcgctcc gggtcagcgg ctcgcctcgc gcttcttcca aaatggaatc
     1921 gaacacgcct tcggccgtca gcgatcggac tgcgctcagg acttgactgc tcagaatgcc
     1981 ttcttgccca agtttgtcga gataagcaaa ggcctccgac aagctttctt tgttccgagc
     2041 ataataatcg cgtccccgac gctgattgct gtaggcctca aactgttcgc aggcaacgag
     2101 gacctccgcc aattgccgca cccaagcctt tgccttagcc agcctttccg gataatagta
     2161 atagagcatt acctgctgca tccaaggttg ccactcgacg ccaagtgtct tcaactttgg
     2221 cttcaccatg cgcaatcgtc tggccaggcg tcgagcatag cccagccgca tttcgatctg
     2281 ctcgacagcc catgtgtcca tgggtacgcc ggaggcgata agatcctggc gatacaacga
     2341 aacgaaggct tctgtctccc ggccataagt ggtcctcggg tgaagggcgc gccattcacg
     2401 aggtctcgtc ggaatgcctc gttgcttggc ccaagaccaa atagttccaa agagttgccg
     2461 gtcgaggccg gcacggccca ggtcatgcaa aaggcaggca acatgatact gccgtacccg
     2521 atcgcccgga tggcctaagc gatcggcgac ggtcacacac atcttcgctg tccgaacggc
     2581 atgcggccga tcatagccgc ggattgttcg tcccggtcga cgagggtgcg gataatcgta
     2641 gagtttcata agggctttcg cgagagagcg agagacttct agtggttcca atcggtggga
     2701 atcagataga gccatagttc acagccacag ctgaacgact gagtagaccg ggcgagaata
     2761 tcgtcggtcg aaatgaggtg tcaaggcgcg gctccgaagg aatcggcgct cgcccaattc
     2821 catagacttt gctatgctca cctatggcac atgtaaggac atggagatga cgaacgggtt
     2881 acggatctgt tgtctcgtcg cggcttcggc catcgtcaca gaaccggtgt gcagctttgc
     2941 gggatcattg gaccaattga ccgatctgac cggacgcgta caggccatcg taacattgaa
     3001 gagtcgcgac aacttcacca gcgaatatcg ctacgatgtc agcgttagaa atctctcatc
     3061 cgacgctatc atcggtgatt cagtggtcat cgtgctggac aagattacca acttggccgg
     3121 cgaagaccga gaagggctca cgggagaatc cttcctgaaa cgattcgacg tgctggacca
     3181 agacggccag acagaagacg ggaaaccata cttccgcgtt ccggttggga cttccccgga
     3241 cctcgcaccg caaaccgata gccttcccgc cgtggtcaga atcaggaatc gggactacgt
     3301 agcagtcttc actccctcgt tcaaagtgct ggggcagaag cgtcctcctc cggaagcaaa
     3361 gcgtgcggag tctccgatat cttctacgcc tccggctcct ggaacgtcg
//
LOCUS       DASTWE010000461         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_788289, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000461 DASTWE010000000
VERSION     DASTWE010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788289"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /gene="rfbB"
                     /locus_tag="VFT30_04880"
     CDS             <1..1071
                     /gene="rfbB"
                     /locus_tag="VFT30_04880"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU5091996.1"
                     /translation="TGAAGFIGSNFVRLTLQETSFHIVIVDKLTYAGHPATLEALSKD
                     GRLTIIQEDITDEQVMQVIFRKYAPTWVVNFAAESHVDRSIDNPEPFIKTNTIGTYVL
                     LEAARRFLAKTDDRGKSDFRFLHVSTDEVFGSLGAEGKFSESSPYAPNSPYAASKGAA
                     DHFVRAYWHTYKVPTLLTNCSNNYGPYQYPEKLIPLTILNALDGKPLRIYGDGLQVRD
                     WLYVKDHCQAILLVLRNGRVGESYNIGGEEERTNREIVDGICATLQEFVPASQNAHLI
                     EKNIGQYADLKRFVEDRPGHDRRYAINPSKIKSELGWAPQHTFETGLRKTVEWYLSHR
                     KWCRQVLGDRYDRQRLGLLAYD"
     gene            1221..>1574
                     /locus_tag="VFT30_04885"
     CDS             1221..>1574
                     /locus_tag="VFT30_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5091997.1"
                     /translation="MLRLGVIGYGYWGPNVVRNFLGHHDCKVITICDKSSAALTRVRA
                     AHPGVGVTTDPDAVLLATDIDAVAIVTPVSYHYELAKKALENGKHVFVEKPFTATSAE
                     AEDLIELADRYGLQIM"
BASE COUNT          389 a          372 c          420 g          393 t
ORIGIN      
        1 acaggtgcgg cagggtttat tggtagtaat tttgtcaggt taactcttca ggagacatcc
       61 tttcatatcg tgatcgtgga taaattgacc tatgcgggac atccagccac tttagaagct
      121 ctctcaaagg atgggcgtct aacgataatt caggaggata tcactgatga acaagtcatg
      181 caggtaattt tccgaaagta tgccccgaca tgggtcgtca attttgctgc agagtcacac
      241 gtcgatcgat ccattgataa tcctgagccg tttataaaaa ccaatacgat cggcacatat
      301 gtacttcttg aagcggcccg tcgattcttg gctaagacag atgatcgtgg gaagtcagat
      361 tttcgttttt tgcatgtttc gacagacgaa gtgtttggct cactgggggc tgaggggaag
      421 ttcagtgaat cgtctcccta tgcgccaaat tctccgtatg ctgcctcaaa aggtgcggca
      481 gaccattttg tccgggccta ttggcatacc tacaaggttc caactttgct gacgaactgc
      541 tcgaataact atggcccata tcaatatcct gaaaagctga ttcctctcac gatactgaat
      601 gctctcgatg gcaaaccttt gcgaatctat ggagatgggc ttcaagtgcg agactggctc
      661 tatgtaaagg accattgtca ggccattttg ctggtccttc ggaacggaag agtaggagaa
      721 agttataaca tcggaggcga ggaagaaagg accaatcgcg agatcgtcga cggaatatgt
      781 gccacactcc aggaatttgt gccggcaagc cagaacgcac atctcattga gaagaacatt
      841 ggccagtacg ctgacctgaa gcgttttgtt gaagaccgtc ccggacacga ccgacgctat
      901 gcgatcaatc cgagcaagat taaatctgaa ttggggtggg ctccccagca tacattcgag
      961 accggattgc gaaagacggt tgagtggtac ctttctcatc gcaagtggtg tcgacaggtc
     1021 ctgggggata gatatgatcg acagcggttg ggtcttttgg cgtatgacta gaatctagct
     1081 caagagccgg tcgccgatct ctccttggtg gatcagccac agtctactga gttcaagtag
     1141 ccgttggttt atccgaccat cctacctttt ctggtggttc caaagaataa ggtctgctcc
     1201 ctctttcaag gagtgccatt atgctacgac tcggtgtgat cgggtacggg tattggggac
     1261 cgaacgtggt ccggaacttt ctcggacatc acgactgcaa agtcatcacg atctgcgata
     1321 agtcttccgc cgcattgact cgtgtgaggg cggcgcatcc cggcgtcggt gtgacgactg
     1381 atccggatgc cgtgcttctc gccacggaca ttgacgcggt ggcgattgtg acgccagtgt
     1441 cataccacta cgaattagcc aagaaagcgc tggagaatgg gaagcacgta ttcgtggaaa
     1501 aaccctttac cgcaacctct gccgaagctg aggacctgat cgaactggcg gatcgatacg
     1561 gactccagat catg
//
LOCUS       DASTWE010000462         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1208230, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000462 DASTWE010000000
VERSION     DASTWE010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208230"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFT30_04890"
     CDS             <1..521
                     /locus_tag="VFT30_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5091998.1"
                     /translation="HKMFCLPEEPLQNTCVGRMSVADNLAFRNFDIEPHAVGGWLISR
                     PALRRSAQDLIGRYNIKPPYPDTPIQSLSGGNVQRAVLARELSADVEILVIANPCFGL
                     DFLAMEEIRLQIVAARNRGTAVLLVSEDLDEILELSDRIVVMFNGQFVYETMSSDADL
                     GIIGRHMTGHTT"
     gene            518..1204
                     /locus_tag="VFT30_04895"
     CDS             518..1204
                     /locus_tag="VFT30_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU5091999.1"
                     /translation="MNTVPASPYPYPLPSRDSGERVALVIIDMQRDFVEPGGFGAALG
                     NKVEHLQKIVPTVAKLLRTFRELRLPVIHTREGHRPDLSDCPPAKKRRGDSTLRIGDP
                     GPMGRILVLGEPGNDIVPELRPEAGELVVDKPGKGAFYATALQERLKELGITHLVFTG
                     VTTEVCVQTSMREANDRGYDCVLVEDGTESYFPEFKQATIRMIKSQGGIVGWVTTADR
                     LTDALRNTYG"
     gene            1399..1800
                     /locus_tag="VFT30_04900"
     CDS             1399..1800
                     /locus_tag="VFT30_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEU5092000.1"
                     /translation="MNQSDVLLAVNGTLMRGLELNSNMVAANATFVRETMTEPTYRLW
                     TINDEHPAMLRVTDGTGVKVAVEVWSVPAEGLAGILLNEPPGLCIGKVRLEDGTIVLG
                     VLGEPALVEKHREITTYGGWRSYVAQRPTAP"
BASE COUNT          444 a          538 c          548 g          371 t
ORIGIN      
        1 aacacaagat gttctgtctc cccgaagaac cgctccagaa cacgtgtgtc gggcggatga
       61 gcgtggcaga caacctggcg tttcgcaact tcgatatcga acctcacgcg gtgggcggct
      121 ggctcatcag ccgccctgcg ctgcggcgaa gtgcacaaga cctcatcgga cgctacaaca
      181 tcaaaccgcc gtatcccgat acgcccattc aatccctttc gggaggcaat gtacagcgcg
      241 cggtcttggc gcgtgagctg tcggcggatg tcgagattct ggtcatcgcc aacccctgct
      301 tcgggttgga ctttctggcc atggaggaaa ttcgcctgca gattgtcgcc gcacgcaatc
      361 gggggacggc ggttcttctg gttagcgaag acctcgacga gatcctggaa ctgtccgacc
      421 gcatagtggt gatgtttaac ggtcaattcg tctatgaaac catgtcgtcc gatgccgacc
      481 tgggaatcat aggacggcac atgacaggac atacaacatg aacaccgtac cggcttcccc
      541 ttatccctat cccttgccgt cgcgagactc cggtgagcga gtggcactgg tcatcatcga
      601 catgcaacgc gatttcgttg aacctggcgg gttcggggcg gcgttgggca acaaggttga
      661 acacttgcaa aaaatcgtgc cgaccgtcgc gaaactcctc aggacgtttc gtgaattacg
      721 attgccggtg attcacacaa gggagggtca tcgccccgat ctctcggatt gccccccggc
      781 caagaaacgg cgcggcgatt caacactccg catcggcgac ccaggtccca tggggcgcat
      841 tctggtcttg ggagagccgg gaaacgatat cgtccccgaa ttgcgtcctg aggccggcga
      901 actcgtcgtt gacaaacccg gcaagggggc attctatgcg accgctttac aagagcgcct
      961 caaagagctc ggcatcaccc atctggtatt tacgggagtc accactgagg tctgtgtgca
     1021 aaccagcatg agagaggcta acgaccgcgg gtatgactgc gtcctcgtcg aggatgggac
     1081 cgaaagctat ttcccggaat ttaaacaggc gacgatccgt atgatcaaat cgcagggcgg
     1141 catcgtcggc tgggtaacga cggcggatcg tctgactgat gccctccgta acacctacgg
     1201 ttaacgacat gggagaactg aaccaatacc gacttcagaa ttagaaatgt ctcaagcaat
     1261 agaaatggag gaattgtcac tgccatggtc tcttcaaaac agcagtgtgg gccatcccac
     1321 catacagaaa tggcaccaca aaggagtctt gtttccactc atcaagacag cgtatcaagc
     1381 aatcacgagg agcgccgcgt gaaccagagc gacgtactgc tcgcggtaaa cggcacactg
     1441 atgcgagggc tcgaactcaa ctccaacatg gtggccgcca atgcaacgtt cgtccgtgag
     1501 acgatgactg agcccacgta tcgcctctgg acgatcaacg atgaacatcc tgcgatgctg
     1561 cgtgtcaccg atgggacagg cgtcaaggtg gcggtcgaag tctggtcggt acctgccgaa
     1621 gggttggcag ggattctgtt gaatgagccc ccaggtctgt gtatcgggaa agtgcgtctg
     1681 gaagacggaa ccatcgtcct cggtgtatta ggtgaaccgg cgctcgtcga aaagcaccga
     1741 gagatcacca catacggagg ctggcgcagc tatgtggcac agcgtcctac cgcgccctga
     1801 catgattcag attgttttca gataacagcg gggcgggagg ccaagggagg cacgccacca
     1861 ccccactggt ccgagccgaa ggcagcctgc tcggacaata c
//
LOCUS       DASTWE010000463         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1563883, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000463 DASTWE010000000
VERSION     DASTWE010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563883"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..899
                     /locus_tag="VFT30_04905"
     CDS             123..899
                     /locus_tag="VFT30_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092001.1"
                     /translation="MTGTEARGATQLPLSSMKRLDCGSAPGPKALKEGVKDPARHVVS
                     LNSSDGLIMLYGHPVVSKIALQALVERALTGHPVVYLDGTHTFDALLIGQLARSRRQQ
                     PRKALAMIHVARAFSTRQLERLMSHCLAGALERYQASTAVISGLLESISADCMTDKEV
                     NRLTDRMIESMRHLIQQGFLLLCPCPSVPMPMAPAYRLFGLLRSMSHRCIRVYEVHGK
                     VVTKEISFDAIPIHTESFEDIRVDSSLPFQSTDQCAFSAR"
BASE COUNT          227 a          282 c          275 g          235 t
ORIGIN      
        1 cggatagaat cgatcgacgg ctttacgcac tcctcggttc aactctgccc aacttgttgg
       61 aatgcctaca gacaacgtca agtcttcgct ggcggatgct gcggatagga gaagggtaag
      121 gcatgacggg cacagaagcc agaggagcaa cccaattgcc gctctcaagt atgaagcgtc
      181 tcgactgcgg gtccgctcct gggcccaagg ctctgaagga aggagtgaaa gatcctgccc
      241 gccatgtggt ttcactgaat tcatctgatg gactgatcat gctgtatggt catccggttg
      301 tctcgaaaat tgctttacag gctctcgtcg agcgcgcact gaccggacat ccggtcgtat
      361 atctggatgg aactcacaca ttcgatgcgt tacttatcgg acaactggcg cgcagccggc
      421 ggcagcagcc tcggaaagcc ctggcgatga tccatgtagc acgagccttc agcacgcggc
      481 aattggaacg gctgatgtcc cactgtttgg ccggcgcctt ggagcgctat caagccagca
      541 cggcggtgat ttccggcctc ctcgaaagca tatccgccga ctgtatgacc gataaagagg
      601 tcaaccggct gacggaccgg atgatcgaat cgatgcgcca cttgattcaa caaggatttt
      661 tgttgctctg cccctgcccc tccgttccga tgcctatggc tccagcctat cggttgtttg
      721 gcctgctgcg ttcaatgagc caccgttgca tccgtgtata tgaagtgcat gggaaagtag
      781 tgacgaagga gatttcgttt gacgccattc ccatccatac agagtctttt gaggacatca
      841 gagtagactc atctctcccc ttccaatcca cggaccaatg cgcattctca gcgagatagc
      901 agccggtgct tctattgctg ccaggtgacc cttcttcgtg ggttgaccaa caaaagtcgt
      961 tggtaatatc tttgacagag gctcgacagc atctcccaac ctgaagcgag caacggggt
//
LOCUS       DASTWE010000464         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_335983, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000464 DASTWE010000000
VERSION     DASTWE010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335983"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1025
                     /locus_tag="VFT30_04910"
     CDS             <1..>1025
                     /locus_tag="VFT30_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5092002.1"
                     /translation="QIRVWIWGQAIRYLVDHPRTVLFGIGYDRRRFVEEVIGLPYEGY
                     NFNYQTAHNLFLDILIKGGIAPLIPLVAACLWLFWVGLKSVLIPIRDSQPTSRLGIAW
                     ALISIWPALMVISFAGEEVLTDNLLLHWTALFGLLLGLSGLALASWLPNRILHMTATA
                     GIGGGPTYITALVRHQIQAGKHVRIFCSDEKPFVEIWREMGIDVSILPMRRPSLQSVW
                     QLLKELLRAPAPIHAHGRGAAFFAVWVKVLVRIPVIYTPHGPHYAQKRGWRFLVEWCF
                     EYMFLVIFDAVLYVSPGEQETARLHHLPVRSSRVVLSGLMREGRTDGECQPTRDALLG
                     ELEIPTH"
BASE COUNT          184 a          294 c          292 g          255 t
ORIGIN      
        1 ccaaatccgt gtctggattt ggggtcaggc gatccgatac ctggtggacc atccccgcac
       61 ggtcctgttc ggcatcggct atgaccgccg gcggtttgtc gaagaagtca ttggccttcc
      121 atacgaaggc tacaatttca attatcaaac cgcccacaat ctctttctcg acattctgat
      181 taaggggggc atcgcccctc tcattcccct ggtagcggcc tgtctctggc tgttctgggt
      241 cggcctcaag agcgtcctca ttccgattcg tgactcgcag ccgaccagcc gtcttgggat
      301 cgcctgggcg ctcatttcaa tctggccggc gctcatggtg atcagtttcg caggcgagga
      361 agtactgacc gataacctgc tcttgcattg gaccgcgttg tttggcctct tgctggggct
      421 ctccggtctc gcccttgcct cctggctgcc gaatcggatc cttcatatga cggcgacggc
      481 cggtatcggg ggtggcccga cctatatcac cgcgctcgtg cggcatcaaa tacaagccgg
      541 gaagcacgtg cgtatctttt gttcggacga aaagcctttc gtcgaaattt ggagagagat
      601 gggcatcgat gtctcgatcc ttccgatgcg ccggcccagc ctccagtcgg tctggcaact
      661 gttgaaagag ctgttgcgcg caccggctcc gatccatgcg catggtcgag gtgccgcttt
      721 ctttgccgtg tgggtcaaag ttcttgttcg cataccggtt atttatactc cgcacgggcc
      781 gcattacgcg cagaagcgcg gctggcgatt cttagtcgaa tggtgcttcg agtatatgtt
      841 ccttgtgata ttcgacgcgg tcctgtacgt ctcgcctgga gaacaagaga cggctcgcct
      901 ccatcatctt cctgtccgaa gctcgcgagt cgtattgtcg gggttgatga gggaaggaag
      961 aaccgatggt gaatgccaac cgacacgtga tgcactgctg ggagagttgg aaatccccac
     1021 gcatc
//
LOCUS       DASTWE010000465         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_239152, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000465 DASTWE010000000
VERSION     DASTWE010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239152"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..413
                     /locus_tag="VFT30_04915"
     CDS             102..413
                     /locus_tag="VFT30_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU5092003.1"
                     /translation="MSTKADYGWLTDLMARWIEDLNKRYPATLRAQGVQGKVMLTGML
                     HENGLLSDVRVVKSSGNTALDEVALEDVRNGPSINLSHPLERTQMSVKFSISYDLKMA
                     Q"
     gene            complement(424..645)
                     /locus_tag="VFT30_04920"
     CDS             complement(424..645)
                     /locus_tag="VFT30_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092004.1"
                     /translation="MDQVTQDVVAGRYGAPHKVDTLPEGRSVWTYYDRGSGTAGYGSY
                     ARPSRCKAYLLTFDQSGVLRDWKQQDCAS"
     gene            complement(776..>1649)
                     /locus_tag="VFT30_04925"
     CDS             complement(776..>1649)
                     /locus_tag="VFT30_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU5092005.1"
                     /translation="VASDKNVGDGAVQAAFDEIKRLEQLLSTWRSNSELSQVNAEAGR
                     RPVQVSRETLQLIIRALDIAELTHGGFNIALGPAIEAWSVTERQRIPEKEELGRLKPL
                     VDWTRIQINREERTIYLPHKGMRIDVGGIGKGYAADRAVEQMKQAGAIGGVVALSGDI
                     KTFGIFPGRSGFPVGIRHPRQEGVLIAMIDLMDEAISTAGDYERFFERDGIRYHHILD
                     PQTLWPARICQSVTVIAKEGTVADGLDTGIFVLGPEQGMALVERLPNVEAIIIDDKGK
                     MTVSSGLRDRLRAP"
BASE COUNT          375 a          491 c          391 g          392 t
ORIGIN      
        1 atagttcaac tagtcgtctt gaatcccagg tcgctacgaa ttccccgcgc ccgttgttgt
       61 cgtcttccgg gaatgcttcg acagcgtcca ccgaccaact catgtcaacc aaagccgatt
      121 acggatggct cactgacctc atggcaagat ggattgagga tctgaataag cgttaccctg
      181 ctacgttgcg ggcccaagga gtgcagggga aggtgatgct gacggggatg cttcacgaga
      241 atgggctgct gagcgatgtg cgcgtcgtca aaagctcagg caacacagcg ctcgacgagg
      301 tggctttgga agacgtaaga aatggccctt ccatcaacct ttcccacccg ctggaacgga
      361 cccaaatgtc ggtgaagttt tcgattagtt atgatttgaa gatggcacag taatcgctcc
      421 cgactaagac gcacagtcct gttgtttcca atcgcgtaat acgccggact gatcaaacgt
      481 caatagatat gctttgcatc tggacgggcg ggcataactt ccatagccgg ccgtgccgct
      541 tcctcgatca tagtaggtcc agaccgatcg cccctccggc aacgtatcta ccttgtgcgg
      601 cgcaccgtat cggccggcca ccacatcctg tgtgacttgg tccaccttgc tttggaaata
      661 tcctgtttcg aactggctac aagcgaacag caccccaaat ccaacacaca gtaaataata
      721 tctcgagcgt tgagttctca tcatcacaga cctcatgagg aagaaggaaa tttgcttatg
      781 gtgcacgtag tcgatcacgg aggcctgacg aaacagtcat cttgccttta tcatcgataa
      841 tgatcgcttc aacattcggc aagcgctcta ccaacgccat cccctgttcc ggtcccagca
      901 caaaaatacc tgtatccaac ccatcagcca ctgtgccttc cttcgcaata acggtgacgc
      961 tctgacatat gcgtgccggc cacagggttt gggggtccag gatgtgatga taccggattc
     1021 catcccgctc aaagaaccgt tcgtaatcac cggccgttga aatcgcttcg tccatcaaat
     1081 cgatcatagc tatcaacact ccctcttggc gtgggtgcct gatgcctaca ggaaacccgc
     1141 tgcgtcccgg aaagatgccg aaggttttga tatctcccga tagggcgact acccccccta
     1201 tggctcccgc ttgtttcatc tgctctacag cccgatcggc cgcatatcct ttgccaatcc
     1261 caccgacgtc gattcgcatt cccttatgcg gtagataaat cgttcgttcc tctctattaa
     1321 tctgaatacg agtccaatcg acgagcggct tcagccgccc taactcctct ttctcaggaa
     1381 tgcgctgccg ttccgtcacg ctccaggctt caatcgcagg gccgagcgca atattgaacc
     1441 caccatgtgt cagctctgcg atgtccaaag ctcggatgat cagttgcaat gtctctcgac
     1501 tcacctgcac aggtcggcga ccggcttctg cattcacctg tgagagttcg ctgttagaac
     1561 gccaggtgct cagtaactgt tcgagtcgct tgatctcgtc gaatgcggcc tgtacagccc
     1621 catcgcccac gttcttatcg gaagccaca
//
LOCUS       DASTWE010000466         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_271031, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000466 DASTWE010000000
VERSION     DASTWE010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271031"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFT30_04930"
     CDS             <1..556
                     /locus_tag="VFT30_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEU5092006.1"
                     /translation="VVAAAGDYVIETQGTTDVVMGLYGPNDMATLIVENDDGGVQGNS
                     RISRNLSLGTYYVRVRHYTSTSTGNYSIGVQQPAARPDIPIIQVNGPPLEGSIDVANE
                     RDLYTFTATVSGTYTIETAGSTDCFLTLFGPGNADTFIAEDDDSGPAANSRIVVNLES
                     GSYFVQVRHYSSTATGPYSILVRR"
     gene            complement(622..885)
                     /locus_tag="VFT30_04935"
     CDS             complement(622..885)
                     /locus_tag="VFT30_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092007.1"
                     /translation="MDHRLFDDSFEGVDDGRMQDVKEVAGVDDRCRAVMTMVFSSEVG
                     EMASIKSITHWRTSLSSSGRSATPLGAAVCSCSVTKMSCSTAV"
BASE COUNT          263 a          305 c          258 g          227 t
ORIGIN      
        1 tgtggtggct gcggccgggg attatgttat cgaaactcaa gggacgacag atgtggtaat
       61 gggactctat ggtcccaacg acatggccac attaatcgta gagaacgacg acggtggagt
      121 ccaaggtaat tcgcggatca gccgaaatct cagtcttggt acctattatg tgagagtgcg
      181 gcactacacc agtacgtcaa ctggcaatta cagcattgga gtgcagcaac ccgcagcccg
      241 gcccgatatc ccaatcatac aggtcaatgg cccccctctt gagggaagca tcgatgtggc
      301 caacgaacgt gatctctaca cctttactgc cacagtttcc gggacatata ccatcgaaac
      361 ggctggcagc acggactgct tcctcacact ctttgggcca ggaaatgcgg acacgttcat
      421 cgctgaggat gatgatagtg gtcctgccgc gaactcgaga atcgtggtca atctcgaatc
      481 tggctcatac tttgtgcagg tcaggcatta cagctcaacc gcgaccggcc catacagcat
      541 tttagtgcgg cgctaatcgc caagaggtga cacaattcga cttcgcgcta tccgacgcag
      601 gctacctcca cgacagaaac cctaaaccgc cgtgctacag ctcatcttcg tgacagaaca
      661 actgcacacc gcggcgccca gcggcgtcgc gcttcggccg gacgagctga ggctcgtgcg
      721 ccagtgcgtt atcgatttga tggaggccat ctcgcccacc tcggacgaaa aaaccatggt
      781 catgacggcg cggcaacggt catcaacacc agcaacctct ttcacgtcct gcatccggcc
      841 atcgtcgacg ccttcaaacg aatcatcaaa aagccggtgg tccactaacc atctctccca
      901 tccaaacatt acaatcaggt caacgtgtac gtaccatggc tcaaaatacg tgcaattagg
      961 acgctgtact acttcgaatg gggtatatgc cgaccatttc cgaccttgat attgtaccgt
     1021 catttttagc tttagcgcag aataacctca tgt
//
LOCUS       DASTWE010000467         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_206639, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000467 DASTWE010000000
VERSION     DASTWE010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206639"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFT30_04940"
     CDS             complement(<1..936)
                     /locus_tag="VFT30_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU5092008.1"
                     /translation="MEPYQVFISYAREDEDHKNRLVNELQTLVHLGLVRLWHDLKIGA
                     GKEYASEIFSNIDRSDIILPLVSPSFLKSKFCRYELDRARLRIKSKKCHVLPILISRT
                     PGWDRIPVGGGILGNLQALPPNGMPLTRWSHPDHAWRAVSRGIGETVLTTSGASMGSD
                     DVKARTLLTVGTFVLDAIGTTFDVEGWQHYYPGDHDAERGLYGEVEVASNANKLTLTG
                     YSNDFKQIETVATIDYEPQVHRFSYSVIEPKQDRGLQYRFDLSISMLGGITVDATRIA
                     ESAAEVDPELTLQGLLDFVRQTFREQKRKYRGGRFR"
     gene            complement(999..>1298)
                     /locus_tag="VFT30_04945"
     CDS             complement(999..>1298)
                     /locus_tag="VFT30_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEU5092009.1"
                     /translation="GGHGIYLDDLFFGLISYDMFFLKTDEHNRGDYVKAKAKPFSFES
                     ARKGLVVTSYWQCPDAVMKDGRKLRQWIGKALDAARRKKTSPHRGERIRRQRRDR"
BASE COUNT          285 a          360 c          353 g          300 t
ORIGIN      
        1 cctgaacctc ccgccgcgat atttccgttt ctgttcgcgg aacgtctgcc tgacaaagtc
       61 gagcaacccc tgcaaggtca gctcaggatc gacctctgcg gccgattcgg cgattcgagt
      121 agcatcgacc gtgatgccac ccaacatcga aatggacaaa tcgaaacgat actgaagtcc
      181 tctgtcttgc ttcggctcga tgacagagta agagaaacgg tgaacttgtg gctcgtagtc
      241 tatggtcgcc acagtctcaa tctgtttgaa gtcattgctg taaccggtca gcgtgagttt
      301 atttgcgttc gatgcgacct cgacctcgcc atagagtccg cgctcggcgt cgtggtcgcc
      361 gggatagtag tgctgccagc cctcaacatc gaatgttgtt ccaatcgcat cgagaacgaa
      421 cgtgccgacg gtcaggagcg tccgggcttt tacatcgtcg gaacccattg acgctcccga
      481 cgtggttagg acggtttcgc caatacctcg agaaacggct cgccacgcat ggtcgggatg
      541 cgaccatctt gtgagcggca tcccgtttgg cgggagcgcc tgcagattac ccaggatgcc
      601 gccgccgacc gggattcgat cccacccagg agtacgagaa atgagaattg gcaacacgtg
      661 acactttttg gacttaatgc gcagcctggc acggtccaat tcataccgac agaattttga
      721 cttcagaaac gatgggctga cgagcggaag gataatgtcg gagcggtcga tattgctgaa
      781 tatttcactc gcatactcct tgccggctcc aatcttgaga tcgtgccata atcggaccaa
      841 accaagatgc acaagagtct gcaactcgtt cacgagccgg ttcttgtgat cctcatcctc
      901 gcgcgcgtag gagataaaga cctgataggg ttccatattg cgtctcgcaa gtatacggtt
      961 gcattccaat caagaatatc acgttgaact agagatcact agcgatctcg tcgttgtcgc
     1021 ctgatacgct ctcctcgatg cggacttgtc ttcttgcggc gcgcggcgtc gagtgccttg
     1081 ccgatccatt ggcgcagctt gcggccgtcc ttcatcacgg cgtccggaca ctgccaatag
     1141 gatgttacga cgaggccctt gcgcgcgctc tcgaacgaga atggcttggc cttggctttg
     1201 acatagtcgc cgcggttatg ttcgtcggtc ttgagaaaga acatgtcata ggagatgagg
     1261 ccgaagaaca ggtcgtcgag atagatcccg tgcccacc
//
LOCUS       DASTWE010000468         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_336068, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000468 DASTWE010000000
VERSION     DASTWE010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336068"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFT30_04950"
     CDS             <1..662
                     /locus_tag="VFT30_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5092010.1"
                     /translation="LAAQAVGANGSVVGIDLAEHMLTVAEKKAKRLGLHNVMFRVGDI
                     TTLPFESASFDAVTSRFCLMFLPEIPKAATEIARVLKPGRWVAVAVWSAPEKNPSISL
                     SMAAVKQVIDLPPTDPMAPGIFRLAKPGDFADMLGRAGLADITDQEFLGEWSYGSTDE
                     YYTSLMEIAAPVQNLMATLSEAQRLEVKHLIIKAASQFQRGARITFPIAVRMVAGRKP
                     G"
     gene            666..983
                     /locus_tag="VFT30_04955"
     CDS             666..983
                     /locus_tag="VFT30_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEU5092011.1"
                     /translation="MSTKSDGFKDFILDQLTELDEVTGRAMFGGYGLYQKAKFFAIIH
                     KGRLYFKVTTATAPRYKEQGMKPFRPNAKQTLKSFFEVPIDVIEDAEALTQWATEAVN
                     ETA"
     gene            1412..1930
                     /locus_tag="VFT30_04960"
     CDS             1412..1930
                     /locus_tag="VFT30_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEU5092012.1"
                     /translation="MSHILWTIGHSTRPIKEFVSLLKAHGVHRLVDVRTVPRSRHNPQ
                     FNTEALAQSLARADIHYRHSGNLGGLRKPKKDSINMGWRNASFRGYADYMQTDEFRKA
                     LDELMAESRLQQTAIMCAEAVPWRCHRSLIADALVTRDWEVRHIMSEAKADPHRLTPF
                     AAVNNGVLCYRG"
     gene            complement(1955..>2539)
                     /locus_tag="VFT30_04965"
     CDS             complement(1955..>2539)
                     /locus_tag="VFT30_04965"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092013.1"
                     /translation="GNNSKPFQATQIQAVGFRIPQTLVTSDPELALDFWERHGRVVYK
                     SLSGVRSIISQLSPAHMRRLDDIAWCPTQFQQYVSGTDVRVHIVGDRLFACEIHSDAD
                     DYRYAGRHGLSVGMRRYDLPSDCVNRCLELCQAMGLAVAGVDLRRTPDDQWYCFEVNP
                     SPGFTYFQTQTGQAVDQAIAELLLQAPIPLGHCA"
BASE COUNT          592 a          720 c          708 g          519 t
ORIGIN      
        1 tcctcgccgc ccaggcagtc ggagcgaacg gcagcgtggt gggcatcgat ctcgccgaac
       61 acatgctcac tgtagcggag aaaaaggcaa aacgattggg cttgcataac gtgatgttca
      121 gggtcggcga tatcaccacc ctgccgttcg aatccgcttc cttcgacgct gtcaccagcc
      181 gcttctgtct catgtttctt ccggaaattc ccaaagcggc tactgaaatc gcacgagtcc
      241 tgaagccggg acgctgggtc gcggtagccg tctggtccgc tccggaaaaa aacccctcga
      301 tcagcctctc aatggcagcc gtcaagcaag tgatcgatct gccgccgacg gatccgatgg
      361 cgccaggcat cttccgtctc gctaaacccg gtgacttcgc cgacatgttg gggagagccg
      421 gccttgcgga catcacggat caggagtttc ttggggaatg gtcttacggt tcaaccgacg
      481 aatattacac gagcttgatg gagatcgccg ccccggttca aaatctcatg gcgacgctgt
      541 cggaggcgca acggctagag gtgaaacatc ttatcatcaa agctgcttcg cagttccagc
      601 gcggtgctcg catcacattc ccaatcgccg tgcggatggt cgcaggacgc aagccaggat
      661 gaggcatgtc gacgaaaagc gacggcttca aagacttcat cttagatcag ctgacggagc
      721 tggacgaggt gaccggacgt gccatgttcg gcggctacgg tctctatcag aaagccaagt
      781 tttttgcgat cattcataaa ggccgtctgt atttcaaagt tacaacagcg actgcacctc
      841 gttacaagga acagggcatg aagccgttcc gacccaacgc caagcagaca ctcaaatcct
      901 tctttgaggt gccaattgat gtgattgaag atgcggaagc cttgacccag tgggcgaccg
      961 aagcagtcaa tgagaccgct taaaaactcg gttaatgccg acaatatagt tggagtttcc
     1021 gtacagtatt aatgcctgaa tatcttcagg atgctcaaaa ggctgttgat ctcacccgcc
     1081 caaccccggc gcgccgagac gcgcctctcc acgggcaaga ctgcagcgag gtccgcgagg
     1141 cgacgaagga gcccgtagcg gacgggcgcg aggttcacgc tgcaccgaat aaggtgaggc
     1201 atgtttgtga acgcccgcga ggtccacgac gcgaagaata atgagcgtca cgtttgtgga
     1261 cgccgccgag tcggtgaggc ggcggtgtct tcgtgagcgg gcggtgtccc gtgagcgccc
     1321 agtgtcttag aagccgagtc gtaatctttg tctgagcggc acgaacgcag ctgacggact
     1381 ttttcagcat cctgctggag atgatcagag catgtcccat attctctgga ccatcggtca
     1441 ttcgacaaga ccaatcaagg aattcgtatc gcttctgaaa gcgcatggcg ttcaccgact
     1501 cgtcgacgtg cggactgttc cacgctcacg ccataacccg caattcaaca ccgaggcgtt
     1561 ggcgcagagc ctggcccgtg cggacattca ctatcgacat tcaggcaatc tcggtggatt
     1621 gcgcaagccc aaaaaagatt ccatcaacat gggctggcgc aacgcaagtt ttcgcggtta
     1681 cgccgactac atgcagacgg acgagttccg gaaagccttg gacgaattga tggccgagag
     1741 tcgattgcag cagacggcca tcatgtgtgc ggaggcagtc ccctggcggt gccatcgatc
     1801 cttgatcgcc gacgctttag tgacgaggga ctgggaagtc cgacatatca tgtcggaagc
     1861 gaaagcagac ccacaccggc tcactccctt cgccgcagtg aacaatgggg tgctgtgtta
     1921 tcgggggtga ggctatcatc cgcgcctcat aggatcatgc gcagtgtccg agcggtatgg
     1981 gtgcctgcag aagcaattca gcgatggctt gatcgacagc ttgaccagtc tgcgtctgga
     2041 aatatgtgaa tcctggggat ggattgactt caaaacaata ccactggtcg tccggtgtcc
     2101 tgcggaggtc gacgccggcc accgcaagac ccattgcctg acacagctcc agacatctgt
     2161 tcacacagtc tgacggcaaa tcgtaacgcc gcatccccac ggacagcccg tgccgtcctg
     2221 cataacgata gtcatcggca tccgagtgaa tctcacaggc aaaaagacga tctcccacga
     2281 tatgtacccg cacatcggtc cccgatacat actgctgaaa ctgtgtcgga caccaagcaa
     2341 tatcgtcgag cctccgcatg tgcgctggag ataactggga aatgatactg cgcacgccac
     2401 tcagcgactt atagaccacc ctgccgtgac gttcccaaaa atccagtgcc agctccggat
     2461 cgctggtcac aagcgtctga ggaattcgaa atcccaccgc ctgaatctgt gtcgcttgga
     2521 agggtttgga attatttcc
//
LOCUS       DASTWE010000469         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1208339, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000469 DASTWE010000000
VERSION     DASTWE010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208339"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2443
                     /locus_tag="VFT30_04970"
     CDS             <1..>2443
                     /locus_tag="VFT30_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092014.1"
                     /translation="LDMIHLDDDGLPSSYPAKPGENGRWSICPENAGSKKYNVHFIQT
                     PMTLADKQGDTPAIVDKDGLIYVLHEEEAQVRKSDIKYPLVVRANIYDCIDWMLTSEW
                     LDDDHINFQASKINTHWHFLQFDNQSSDGVITGFSYEQSVRPFTMLEKKVKKGLPVPM
                     NTVFTKAAKKGDRVITVKNAAQYHPNVEILIGADNVEGNEVGRVKSIQGNQITLYRPL
                     KNAHPANDIVTVEFVRQRFWADSDVGTVFWHDHALGRVTWPHGGFGTLITEPVGSTYH
                     NPKTGKEIRSGPIADIRTAEPVGYGVNGSFRELMVQLNDTVPHTVNIVTAGNPPGQPI
                     EVALEAGKTVSFPMPEKIPMSPMPFLNGGTHTTGGGLNFKAEPISSRLAANPDSSKLF
                     SSAAHGDPYTPMVRAYLGDTVVFRLLQTMANETMVWTVSGHTYLTERYAGDANRKNSI
                     HIGIAERYDLVVPQAGGPRLQPGDYIHFNGRMSKFSEGAWGIMRVLDKETPDLQKLPA
                     GYSRRNEIPKPLPVCPSEAPVKSFNVVALDYPAMKLNPKAPDAIEVDFERTIQMVNPN
                     AKIYALEEEATKVAGGVHPMPLTLRANVGDCLKVKLTNRMKESRASFSAIGLAFDPKD
                     SLGANVGNNSGDQTVAPGESRTYTYYADPFLGETASLVWDWGNVMVNPRNGLYGAVLI
                     GPKGAQYRDPKTGADISTKNSWVADVIVDRTIQGYENRVNYRDVALFFQDEDNIIGTS
                     FMPYIQNTAGLTAVNYRSEPYKFREEKGCTLGKVFQPCVVDKPEDPATPIIEAHAGDP
                     VRIHVFGASNEQNGMF"
BASE COUNT          577 a          647 c          732 g          487 t
ORIGIN      
        1 ctggatatga ttcacctcga cgacgacggg ttgcccagtt cctatccggc aaaaccggga
       61 gagaatggac gctggagcat ctgccccgag aacgcgggct ccaagaaata caacgtgcat
      121 ttcattcaga caccgatgac cttggccgat aagcagggcg atacgccggc catcgtggac
      181 aaggatgggc tgatctatgt gctgcacgag gaagaagctc aggtccgcaa aagcgacatt
      241 aagtatccat tggtcgtgcg cgccaatatt tacgattgta tcgactggat gctgaccagc
      301 gagtggctgg acgacgacca tatcaacttc caggcatcga agatcaatac gcactggcac
      361 ttcctgcagt tcgacaatca atcgtccgac ggtgtcatca ccggtttttc atatgaacaa
      421 tcggtgcgtc cgttcacgat gctggagaag aaggtgaaaa agggcctgcc tgtgccgatg
      481 aatactgttt tcaccaaggc agcgaagaag ggcgatcggg tcattacagt caagaacgcc
      541 gcccaatacc acccgaacgt ggaaattctg atcggagcgg ataacgtcga aggtaacgag
      601 gtcggtcgag tcaaatcgat ccaaggtaat cagatcacgc tctatcgccc gctgaaaaat
      661 gctcatccgg ccaacgatat cgtgacggtg gagttcgtcc ggcagcgatt ctgggcggat
      721 tccgacgtgg ggacggtctt ttggcacgac catgcgctgg ggcgtgtcac gtggcctcat
      781 gggggattcg gcactcttat caccgagccg gtcggttcga cctaccataa tcctaagacc
      841 ggtaaggaga tccggagcgg accgattgcc gatatccgaa cggcggagcc ggttgggtat
      901 ggagtgaacg gcagcttccg ggaattgatg gtgcagctca atgacaccgt gcctcatacg
      961 gtgaatatcg tcacggcggg taatccgcct ggacagccga tcgaagtggc gctggaagcc
     1021 gggaaaacgg tgtccttccc gatgccggaa aaaattccca tgtcgccgat gcccttcctc
     1081 aacggtggaa cgcatacgac gggtggtgga ttgaatttca aggccgagcc catttcgagt
     1141 cgcttggccg cgaatcccga ttcgtccaag ttgttcagca gcgcagcgca tggcgacccc
     1201 tatacgccga tggtacgtgc gtacctgggc gatacggtgg tgttccggct gcttcagacc
     1261 atggcgaacg agacgatggt gtggacggtc tcaggccata cgtatctgac tgaacgttat
     1321 gcgggcgatg cgaatcgaaa gaactcgatt catatcggca ttgccgaacg gtacgatctg
     1381 gtggtgccgc aagcgggtgg gccacggctc cagcccggag actacatcca cttcaatgga
     1441 cgcatgtcca aattctcaga gggtgcctgg ggcatcatgc gtgtgttgga caaggagaca
     1501 cccgacctgc agaagctccc tgccgggtac agtcgccgaa atgagattcc gaagcctcta
     1561 ccggtgtgcc cgtcggaggc tccggtgaag agtttcaacg tcgtggcatt ggattatccg
     1621 gcaatgaagt tgaaccccaa ggcgcctgac gctatcgagg tcgatttcga acggacgatc
     1681 cagatggtca atccgaacgc gaagatttac gcattggaag aagaagctac gaaggtagcc
     1741 ggaggcgttc atccgatgcc cctgaccctg cgggcaaatg tcggggattg cctcaaagta
     1801 aagctgacca accggatgaa ggagagcaga gcttcgttct ccgctatcgg tttggccttc
     1861 gatcccaagg attcgctcgg tgcgaatgtt gggaacaatt ccggcgatca gaccgtcgca
     1921 ccgggagaga gccgcacgta cacctactac gctgacccat tcctgggaga gaccgcttca
     1981 ttggtgtggg actggggtaa tgtcatggtc aatccgcgga acggtctcta cggagctgtg
     2041 ctgatcggtc cgaagggcgc ccaataccgt gatccgaaaa cgggagcgga catttcgacg
     2101 aagaacagct gggtggccga tgttatcgtc gatcggacga ttcaaggtta tgagaatcgc
     2161 gtgaactacc gcgacgtagc cctgttcttc caggacgagg acaatatcat cgggaccagt
     2221 ttcatgccgt atatacagaa caccgccggc ttgacggcgg tcaactaccg atcggagccg
     2281 tataaattcc gcgaagagaa gggctgcacc ctcggcaagg tgttccaacc ctgtgtggtg
     2341 gacaaaccgg aagatccggc gacgccgatc atcgaggcgc atgccggaga ccccgttcgt
     2401 atccacgtgt tcggtgcgag caacgaacag aacgggatgt tca
//
LOCUS       DASTWE010000470         1364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_271112, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000470 DASTWE010000000
VERSION     DASTWE010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271112"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFT30_04975"
     CDS             complement(<1..334)
                     /locus_tag="VFT30_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5092015.1"
                     /translation="MTWDYERDVLIDVIRKAGKEALRFVVDGFETIQKPDQSPVTSAD
                     LAVNHTLQSRLMSAFPQDGWLSEESPDGLDRLQKRRVWVVDPIDGTKAFISGEPEFCI
                     SVALIELGQ"
     gene            428..>1364
                     /gene="corA"
                     /locus_tag="VFT30_04980"
     CDS             428..>1364
                     /gene="corA"
                     /locus_tag="VFT30_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEU5092016.1"
                     /translation="MKLVQKRSKKTGLSPGTLIHIGEQRTEAVTITLFNYAGARCEEH
                     AVTDMNQLRLPSDETVTWVNVGGVHKVDVLEVFGKHFGLHPLLLEDIANTDQRPKLDD
                     YETYFFLVMKMLMLSERGDIVVEQVSFVLGRNYVLSFQENGADVFHSVRDRLRGGKGR
                     LRQNGSDYLLYALIDAVVDQYFAVLEMLGERIESLQERVIADPKPDTLKDIHTLKRQL
                     LFVRRAVWPLREAMNNLSRSECPFFREPTKVFFRDVYDHVVQIVDTIETLREMVSASL
                     DIYLSSVSYRLNAVMRVLTVITTIFMPLTFIAGIYG"
BASE COUNT          316 a          360 c          371 g          317 t
ORIGIN      
        1 gttgacccag ttcaatgagg gcgacagaga tgcaaaactc cggttcacca ctgatgaacg
       61 ccttcgtccc gtctatcggg tcaaccaccc agactcgtct cttctgaaga cggtccagac
      121 cgtctggcga ttcctctgag agccatccat cctgaggaaa cgccgacatc aggcgagatt
      181 ggagagtgtg gttcaccgcc aggtccgccg aggtcaccgg cgactggtcg ggcttctgaa
      241 tcgtctcaaa tccatcaacg acgaatcgta aggcttcctt gccggccttc cggatcacgt
      301 cgatgagaac atcacgttcg tagtcccacg tcatgatgga agagtagcag gcctgaagac
      361 agatgaaaag gaatgaccgt gtgcccgact tgacctggcc ccagaatctg cgtacaagca
      421 cgttacgatg aaactggtgc agaaacggtc caagaagacg ggactctcgc ccggaacgct
      481 cattcacatc ggtgaacagc gaaccgaggc tgtgactatt accttgttca actatgcagg
      541 tgctcgatgc gaggagcatg ccgttacaga catgaatcaa cttcggctgc cttccgacga
      601 aaccgtcacg tgggtgaatg tcggtggagt acataaggtc gatgttctgg aggtcttcgg
      661 aaaacatttc ggtctccacc cgctccttct ggaagacatc gctaatacgg accagcgccc
      721 gaagctcgat gactatgaga cctatttctt tctggtcatg aagatgctca tgctttctga
      781 gcgaggggac attgtggtcg aacaggtaag tttcgtcctt ggacgcaatt atgttctgtc
      841 gttccaagaa aacggggcgg atgtctttca ctcggtgcgg gatcggctga gaggcggtaa
      901 ggggcgtctt cggcaaaatg gatcggacta tctgctgtat gcactcatcg atgccgtggt
      961 cgaccaatat ttcgctgtgc tcgaaatgct cggagaacgg atcgaatcgc tgcaagagcg
     1021 ggtgatcgcc gatccgaaac cggacacgct caaagacatt cacacgctca aacggcaact
     1081 gttgttcgtg cggcgcgccg tgtggcccct gcgggaagcg atgaacaacc tgtctcgttc
     1141 ggaatgccct tttttccgtg agcccactaa agtatttttt cgagatgtct acgaccatgt
     1201 ggtacagatc gtcgatacga tcgagacatt gcgtgaaatg gtctcggcga gtctcgacat
     1261 ctatctttca agtgtcagtt atcgactcaa cgccgtcatg cgagtgctga cggttatcac
     1321 gacgattttc atgccgctca cctttattgc gggtatctac ggca
//
LOCUS       DASTWE010000471         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1434864, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000471 DASTWE010000000
VERSION     DASTWE010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434864"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFT30_04985"
     CDS             complement(<1..626)
                     /locus_tag="VFT30_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5092017.1"
                     /translation="MHINAQFKESILIGFTILSLAGLFIFDIYTPTAFADHAVYVIVV
                     LIATASRFSWMPSLAAGAGTILTMVGGLGTPWFAGLPRWIQMGNRSITIVILWVLVWF
                     AWKRRQAKTALQKANEDLEQKVTERTQELATVNRTLVTEIAERVQTEQALRLSQGRLA
                     DILDIADDAIIVIEHDRSIMLFNQGAVKLFGYDPDEVLGNPIDLLLPER"
     gene            complement(857..>1166)
                     /locus_tag="VFT30_04990"
     CDS             complement(857..>1166)
                     /locus_tag="VFT30_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092018.1"
                     /translation="PVRAEPHDPRLPEPVDLVFSCDTYHQMNNRVAYFRSLASSLRPG
                     GRVAILDSHPRGFVSGLFGHRVQKEEVRREMEDAGYLLVDDYDLIDRQHFQIFAKSDN
                     "
BASE COUNT          289 a          285 c          326 g          266 t
ORIGIN      
        1 cgttcgggta gcaacaggtc gatcgggttc ccaagcactt cgtccggatc gtaaccgaac
       61 agcttcactg ctccttgatt aaagagcatg atcgatcgat catgctcgat aacgatgatg
      121 gcgtcgtcgg cgatgtctag gatatccgca agccgacctt gcgagagacg cagagcctgt
      181 tcggtctgaa cgcgttcggc gatttccgtt actaaggtcc gattaacggt ggcgagttcc
      241 tgagtccttt ccgtaacctt ttgttcaaga tcttcattcg ccttctgcag ggctgttttc
      301 gcctgtcgcc gtttccaggc aaaccaaacg agcacccata aaatcacgat cgtgatggac
      361 cggtttccca tttgaatcca tcgcggtaaa ccggcaaacc acggagtccc aagtcctcca
      421 accatggtga gtatcgttcc cgctccggca gcgagagacg gcatccacga aaaacgtgaa
      481 gccgtggcga tcaaaacgac tatgacatag accgcatggt cggcgaatgc cgtaggcgtg
      541 tagatatcga agataaataa cccggccagg gacaggatcg tgaagccgat cagtatagat
      601 tctttgaatt gagcattgat atgcatgctg tcgcaatgag ctattgaaac gataattcct
      661 tccggtacca agatgggttg cggttctacg taaggtaccg aggatgggct gctggcgtca
      721 aatgggttgg gtcgacaggt tggacagtca ccatggcgac gagatgtttc aagaagtgac
      781 tatgttaatc gaggatcgcg agtaacatcc gggtcgttaa gcaacgatcc atcgagaagc
      841 agaaagttat ggattctcag ttatcggact tcgcaaaaat ctggaagtgc tggcggtcga
      901 tgagatcgta atcgtctacc aggagatagc cggcatcttc catttcgcgt cggacctctt
      961 ctttctggac tctatgaccg aataggccgg aaacgaatcc gcgaggatga gaatcgagaa
     1021 tcgcgacgcg gccgccgggc cgtagcgatg aagcgagaga tcgaaaatag gcgacgcggt
     1081 tgttcatctg atggtaagta tcacaactga acaccaagtc gacgggctct gggagtcttg
     1141 gatcatgcgg ttcggcccgg accggt
//
LOCUS       DASTWE010000472         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1467139, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000472 DASTWE010000000
VERSION     DASTWE010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467139"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..>1142
                     /locus_tag="VFT30_04995"
     CDS             82..>1142
                     /locus_tag="VFT30_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5092019.1"
                     /translation="MIQNVHELQQKVEHAEHVKRITIQIHAASNLDQILLDLHKDILS
                     LFDAEDLTLFAFDSEKKEIFSKVPHIDGIEEVRIPITEQSLAGFCAKYLRPVNIADAY
                     NIAELRAIHPSLLHDTSYDKRTGFKTKQVLTYPIVADNKYLMGVLQLFNKKNGSRFTR
                     KDEEAVDDIAKALGIAFFNIRKILKKNPTKFDLLVGSNRITQNELDQAVADSRKGMSD
                     LESILIEKYKVTKLDIGRSLAQFYKCPYIEYSERTLVDVELLKNLNVDYLKKNHWMPL
                     KRDRTTIEILTDDPGDLDRVQDIKRTFPGLNIRFAVSLRRDIAQFLSSATGQSDSGGR
                     KLDENVSDILGELVTEAQA"
BASE COUNT          322 a          320 c          261 g          239 t
ORIGIN      
        1 ggcgcctgct gatccggtgt atgaccggat tcattcacgt ctcgacctca gagaaaatac
       61 agtcggatgc acagcaagcc gatgattcag aatgtccacg aactgcagca aaaggtcgag
      121 cacgcggagc acgtcaagcg cattaccatc cagattcatg cggcaagcaa tctagaccaa
      181 attctcctcg acctccataa agacatcttg agtctgttcg atgcggaaga tctgacactg
      241 tttgcgttcg actcagaaaa gaaggaaatt ttctccaaag ttcctcacat cgacggcatt
      301 gaagaagtcc gcatccctat taccgagcag agtctcgccg gtttctgcgc caaatatctc
      361 cgccccgtca atatcgccga tgcctacaat atcgccgaac tccgtgcgat ccatccgtct
      421 ctgctccacg acacgtctta cgataaacgc accggcttca agaccaaaca agttctgacc
      481 tatccgatcg tcgccgacaa caaatacctg atgggagtcc ttcaactttt caataaaaag
      541 aatggaagtc ggtttacccg aaaagacgaa gaagccgtcg acgatatcgc taaagcgctg
      601 ggaatcgctt ttttcaacat ccgaaagata ttgaaaaaga atcccacgaa gttcgatctc
      661 ttggtcggca gcaatcggat cactcagaac gaactcgacc aggccgttgc cgactcccga
      721 aaagggatga gcgatctgga aagcattctg atcgaaaaat acaaagtcac caaactcgat
      781 atcggcagat cgctcgcaca gttctataaa tgtccgtata tcgagtacag cgagcgcacc
      841 ctcgtcgatg tggaactgct gaagaatctc aatgtcgact acctgaaaaa gaaccattgg
      901 atgccgctca agcgagaccg cacgacgatc gagattctga ccgatgaccc gggtgacctt
      961 gatcgcgttc aagacatcaa gcggacgttc cctggcctca acattcggtt cgccgtcagt
     1021 cttcgacgcg acatcgccca atttctgagt tcggctaccg gacagagcga cagtggagga
     1081 cgcaagctcg acgaaaatgt ttccgacatt ctcggcgaac ttgtcaccga agcacaggcc
     1141 ga
//
LOCUS       DASTWE010000473         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1434878, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000473 DASTWE010000000
VERSION     DASTWE010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434878"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFT30_05000"
     CDS             <1..183
                     /locus_tag="VFT30_05000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5092020.1"
                     /translation="PWVGTMVARDRTGVYEYLPASIRTFPDQERLCQVLRDAVFRQVS
                     YKNLSGGIVAIHVATK"
     gene            266..1888
                     /locus_tag="VFT30_05005"
     CDS             266..1888
                     /locus_tag="VFT30_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5092021.1"
                     /translation="MNSILYIFLPCKKVYPIGITYLADFIHRRKPEVRQRILDLSLFP
                     VGQRSQAIRDTATEFKPDLVCFSWRDIQIFSPHEGDTSLEHAFNFYFASNPLKRIAAS
                     VEGVKQLYRYYSHIYTILSYPALIRKEFSKAQIMIGGGAFTAFADQLIERLPEGTIGI
                     LGEGEDAILKVLEGRSIEDERYIVKEGSQIRKGRQGSPALLDALTVDLPYLTSIFPQY
                     GEYISESIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPPDMVVKDIAQHYHQWGARRVW
                     FTDAQFITGKEAYPQCTEILERIVAEKLDIEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQS
                     VESYKAVASIIGEERVFPLMFFLGIQPNTDLEQRLLEEGYLSAGYNPLMLTPTSIRKL
                     LYNPAPLNKIIAKACLRAWERKQGSRDPRRWTGSLSQTSESPTYADQNLSRGIEGNSG
                     RDALLSLEEIIRSRRSSPSPSQAAEPRASSAG"
     gene            2077..>3813
                     /locus_tag="VFT30_05010"
     CDS             2077..>3813
                     /locus_tag="VFT30_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5092022.1"
                     /translation="MYKTIYIPVDNSDHSNTAVDVGVHLAKTFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPV
                     ERRSLEGRNWKVLAEDINTNGYDLVIMGALGVGAVKDSVIGSNTERVVRRIRNSDMLI
                     IKNVQPITGGKIVVAVDGSPYSFGGLMTGLALGKAFNMPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEIIDSGLAKIYQSHLDISREIAQAEQTDLKTTLLDG
                     KAFEKIIQYVRKDVPTLLIVGRIGVHSDDDMDVGSNTENLLRSAPCNVLISNRKYVPP
                     IDTQAEYTIAWTEEALRRMEKIPVFARGVAKTAIHRYAIEKGHTIISNTVVDAAVGHI
                     LPKGAMDAMRALGGSLDAAGIDRDKMQADEAVTKDLMGPTLSGIMTQIVEEKPKEINA
                     STQAYLDRMGQTYFVCDGCGYIGKGETPVKCPVCSADGTKFKQVDKKVFEVAATSEGE
                     LETDVAYDDVPMQWTKDAKEAIRAVPAGFQRRRAKARIEKSARKLGMTTITLEYAAPM
                     IKEAAAEDYTPIF"
BASE COUNT          936 a         1031 c         1021 g          825 t
ORIGIN      
        1 ccctgggtcg gcacaatggt cgcccgcgac cgcacgggcg tctacgagta ccttccggcg
       61 tcgatccgga catttcctga ccaagagcgg ctgtgccagg tcctacgcga tgcggtgttt
      121 cgtcaggtgt catacaagaa cctcagcggt ggaattgtcg ccattcatgt agcgaccaaa
      181 tgaaaagctt atagtttagg cctatggcac aaacccgtga agacggatct ttttgtgtca
      241 ttcgtcatta gcttcagctc aaattatgaa ttccattctc tatattttcc tcccctgcaa
      301 gaaggtctat cccatcggaa tcacctactt agcggacttt atccatcggc gaaagccgga
      361 ggtccgtcag cgcattctcg atctctcgtt gtttccagtc gggcagcgaa gccaggccat
      421 ccgcgataca gccacggagt tcaagccgga cctggtctgc ttttcttggc gtgatattca
      481 gatcttttcg ccccatgaag gcgacacctc gctggaacac gcattcaatt tttactttgc
      541 cagtaacccc ctcaagcgca tcgcggcgtc agtcgagggc gttaagcagc tctatcgcta
      601 ctacagccat atctatacga tcctgtccta tcctgcactg atccgcaaag agttctccaa
      661 agcccagatc atgatcggag gtggggcttt tacggccttc gccgatcagt tgatcgaaag
      721 gcttccggaa ggaaccatcg gcatcctcgg cgaaggagaa gacgccatac tgaaggtcct
      781 tgaaggccga tccatcgagg atgagcggta catcgtcaaa gaaggaagcc agattcgaaa
      841 aggccgacag gggtcgccgg cgctgctcga cgcgctgacc gtagatttgc cctatctcac
      901 gtcgatcttt cctcagtacg gcgaatatat cagtgaatca atcggggtgc agtcgaaacg
      961 aggctgtccc tacgactgcg ccttctgcct ctatccgtac attgaaggca agcgagtccg
     1021 atatcggcct cctgacatgg tcgtcaagga cattgcccaa cattaccacc agtggggtgc
     1081 ccgtcgagtc tggtttaccg acgcccaatt catcacgggg aaagaagcct atcctcaatg
     1141 cacggaaatc ctcgagcgga tcgtcgccga gaaactcgat atcgagtggt ccggctacat
     1201 cagaacctcg ctgattaccc cggaactagc gaaattgatg gtccgatcag gcgtgggtga
     1261 tctggaggtg gccattacgt ccggctcgca agaggttctg aataatctcc acatgggatt
     1321 caagctggaa cggttgtatg acggttgtcg ttatctggcg gaagccggat tcaagggcaa
     1381 ggtgatcctg aattattcgc ttaacagtcc caaagaaacg gaagagagtc ttctacaaag
     1441 cgtcgagtcc tacaaggcgg tggcctcaat aatcggggaa gagcgtgtat tcccattgat
     1501 gttcttctta ggcattcagc caaacaccga cttggaacaa cgactgttgg aggagggata
     1561 cctctcggcc ggttataatc ccttgatgct gactccgacg agtatccgaa agttactcta
     1621 taatcctgcc cctctcaaca agattatcgc caaggcctgt cttcgggctt gggaacggaa
     1681 gcaaggaagc cgtgatcctc gtcgatggac cggatctctg tcccaaactt cagaatcgcc
     1741 cacctatgcc gaccagaacc tcagtcgggg tattgagggc aattcggggc gggacgcact
     1801 cctctcactc gaagagatca ttcgctcacg ccgatcaagt ccatcccctt cacaggcggc
     1861 tgaaccaagg gccagctcag ccggttgatt tttcatctga catcactgag tcacgctatt
     1921 tgactcgtgt cgcgccaccc ggtgccttgc attccagttt cagcgagtgc tatcatccac
     1981 tttctttaga gtgagaatta atacgttaat cgtttggatt ttcatacaat tgcacgtttg
     2041 ctcctaatcc agatcgcgcc ttaaggaggc gcctcaatgt ataagaccat ctacattccg
     2101 gtcgataact ccgaccattc caatactgcg gtggatgtcg gcgtccactt ggccaaaact
     2161 ttcggctcca aaattgtggg aagccatgtc tatgcagcga agatgcatga caagcgtttc
     2221 aagcagatgg aagccggatt gccggaggaa taccacgatg aaaaggagct cgaccggcag
     2281 cgccagatcc atgattcgct gatcacccgc ggtctccaaa tcatcaccga ttcctacctc
     2341 gactacgtcg acaagaaatg taacgaagcc aatcttccag ttgaacggcg atcgctcgaa
     2401 gggcgaaatt ggaaggtact ggccgaggac atcaacacca acgggtacga cctcgtcatc
     2461 atgggagcat taggtgtcgg ggcggtcaag gacagcgtca tcggaagcaa cacagagcga
     2521 gtggtccgcc gaatccgtaa ctccgacatg ctgattatca agaacgttca accgataacc
     2581 ggcgggaaga tcgtcgttgc cgttgacggc agcccctact cgttcggcgg actgatgacc
     2641 ggcttggcac tcggcaaggc attcaacatg ccggtggaag cgatttcagc atttgatccc
     2701 tatttccact acgcggcatt ccacagcatt tccggggtcc ttaacgaaga agccggcaag
     2761 gtgtttcgtt tcaaggaaca ggaaaagctg cacgaagaaa ttatcgacag cggcttggca
     2821 aagatctatc agtctcatct ggatatttcc cgtgaaatcg ctcaggccga acagacagac
     2881 ttgaagacca cgctgctgga cggcaaagcg ttcgaaaaaa tcattcagta tgtccgcaaa
     2941 gacgtcccga ccttgctcat cgtcggacgc atcggcgtcc atagcgacga cgacatggat
     3001 gtcggcagca acaccgaaaa cctactcagg agcgccccct gtaatgtact gatttccaac
     3061 cgtaaatacg tgcctcccat cgatacgcag gcggagtata cgattgcatg gacggaagaa
     3121 gccttgcggc ggatggaaaa gatcccggtc tttgcacgag gggtggcgaa gacggccatt
     3181 catcggtatg ccatcgaaaa aggccatacc atcatcagta acaccgttgt cgatgccgca
     3241 gtagggcata ttctgcctaa aggtgccatg gatgccatgc gggcgcttgg tgggagcctg
     3301 gacgcggcgg gtatcgaccg cgacaagatg caggccgacg aggccgtgac gaaggatctc
     3361 atgggtccca cgttgagcgg aatcatgacc cagatcgttg aagagaagcc gaaggagatc
     3421 aatgcgtcca cgcaggctta cctggaccgt atgggccaga cctatttcgt ctgtgacggc
     3481 tgcggctaca tcgggaaggg agaaacccct gttaaatgtc cggtgtgcag tgcggatggg
     3541 accaaattta aacaggtgga taagaaggtt tttgaagttg ccgcgacatc tgaaggggag
     3601 ctggaaacgg acgttgccta tgacgacgtg ccgatgcaat ggaccaagga tgcgaaagaa
     3661 gcgatccgcg ccgtgccggc cggctttcag cgcaggcgcg cgaaggccag aattgaaaag
     3721 agcgcgcgta agttgggaat gacgaccatc acgctcgaat acgcggcacc gatgatcaag
     3781 gaagccgctg ccgaggacta cacccccatt ttt
//
LOCUS       DASTWE010000474         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_530081, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000474 DASTWE010000000
VERSION     DASTWE010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530081"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..803)
                     /locus_tag="VFT30_05015"
     CDS             complement(153..803)
                     /locus_tag="VFT30_05015"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEU5092023.1"
                     /translation="MADQAVGIPFAKEGIPFIAVPAGATLLTGWLGWPVVACVGAIVT
                     LFSAWFFRNPARVVPQGPKLVVAPGDGKVIAIEEEFEPRYLKERSVRLTIFLNVFDVH
                     INRVPCDGVVEDMQYQPGLFLVASKPEATLRNEQNAMMIKTHEGVKVLCVQVAGLIAR
                     RIVCWISPRDRAIRGERYGLIRFGSRMDTFLPMDTKLRVAVGERVKGGETILGELP"
     gene            complement(813..1826)
                     /gene="ilvC"
                     /locus_tag="VFT30_05020"
     CDS             complement(813..1826)
                     /gene="ilvC"
                     /locus_tag="VFT30_05020"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5092024.1"
                     /translation="MKIYYDKDADIQQIRNKTVAVIGYGSQGHAHALNLKESGVSVVV
                     GLREGASWKKAEQSGLKVMPVADAVKTSEIVMILAPDEGQAAIYRQEVAPNLKAGAYL
                     AFGHGFNIHFGQIVPPASINVFMVAPKGPGHLVRSEYTKGSGVPCLLAIHQDPSGTTR
                     QVGLAYASAIGGGRAGVIETNFREETETDLFGEQAVLCGGLTSLIQAGYETLVEAGYS
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRVVTEQTKQEMKKI
                     LEEIQTGRFAKEWVLENQANRPVYNALLAKGEAHPIEAIGTKLRGMMPWLKKDQLVDK
                     TKN"
BASE COUNT          445 a          615 c          487 g          456 t
ORIGIN      
        1 ggccgcttcc atgtagttgg cattgaagac ggacaatatc gcaaatatgc cgcaaaacaa
       61 attgccggtg gtgaacaaat tcgggatgag atgcatggcg gctttccgcc gtttcccctc
      121 tttcccaaac gaattcctga atcctgccgt tttcatggca actctcccag gatggtctcg
      181 ccacccttca cccgttcacc aacagccact cggagcttag tgtccatcgg aagaaaggta
      241 tccatgcgcg atccgaaacg gatcaaccca tatcgttcac ctcgaatagc tcgatcccgc
      301 ggcgagatcc aacacacaat gcgccgagcg atcaatccag ccacttgcac gcagagaacc
      361 ttcacgcctt catgcgtctt gatcatcatg gcgttctgtt cgttcctcaa cgtagcttcc
      421 ggcttgctgg cgaccaaaaa cagacccggt tgatattgca tatcttcgac caccccatca
      481 catggcaccc tattgatgtg aacatcgaag acgttcaaaa aaatcgtgag cctgacactg
      541 cgctctttca ggtagcgcgg ctcaaactcc tcctcgatgg cgatcacttt cccgtcgccg
      601 ggagcgacca ccaactttgg accttgtggt acaacccgag ccggattccg aaaaaaccag
      661 gcagaaaaca atgtcacaat ggcgccgaca caggcgacga ccggccaacc cagccatccc
      721 gtcaacagtg tagcgccggc gggaactgcg atgaagggaa ttccttcttt ggcaaaaggg
      781 ataccgaccg cttgatccgc cacagaattg cctcagttct tggttttatc gacgagctga
      841 tccttcttca gccacggcat catcccgcgc agtttggttc cgatggcttc gatgggatgg
      901 gcttcgcctt tagcaaggag cgcgttgtag accggtcgat tggcttgatt ctctagaacc
      961 cattctttgg caaaccgtcc tgtctggatc tcttcgagaa tcttcttcat ctcctgcttc
     1021 gtctgttcgg tcaccacacg cggacctctg gtcacatcac cgtatttcgc cgtcgtactg
     1081 atcgagtagc gcatgttggc aatcccacct tgatagatca gatcgacgat gagcttcact
     1141 tcgtgcaggc attcgaaata agccatctcc ggcgagtacc cggcttcgac cagggtctcg
     1201 tatccggcct gaatcagcga cgtcagccct ccgcagagca cggcctgttc gccgaaaaga
     1261 tcggtctcgg tttcctcgcg aaaattcgtc tcgatgaccc cggctcgtcc accgccgatc
     1321 gcactcgcgt aggccaatcc gacctgcctc gtggtgccgc tgggatcctg atggatcgcc
     1381 agaaggcatg gcaccccgct gcccttcgta tactcggaac gaacgagatg tcccggccct
     1441 ttaggggcta ccatgaagac attgatggag gccggcggca caatctgccc gaagtgaata
     1501 ttgaaaccat gaccgaatgc caaataggct ccagccttga gattaggcgc cacttcttgc
     1561 cgatagatgg cggcttgccc ctcatcgggc gccagaatca tcacgatctc ggaagtctta
     1621 acagcatcgg cgacgggcat gaccttgagt ccgctctgct cggctttttt ccacgaagca
     1681 ccctcacgca acccaacgac gaccgacaca ccgctttctt tcaggttaag cgcatgggca
     1741 tggccctgac ttccataacc gatcacggcc acggtcttgt ttcggatctg ctgaatatcg
     1801 gcatccttat cgtagtaaat tttcatgacc aaccctttcc ctcatctcag tatatagcaa
     1861 aacttggcgc gtagcaggat gttagtcgcg ggcaaccttc tttgcttgaa ttgcagtagg
     1921 acgaaccggt tcccgtgcga cggccacccg accggtgcgg accaactctt tgatcccgag
     1981 cggctggagc aagttgatga tcg
//
LOCUS       DASTWE010000475         1100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1596540, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000475 DASTWE010000000
VERSION     DASTWE010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596540"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1100)
                     /gene="lptD"
                     /locus_tag="VFT30_05025"
     CDS             complement(<1..>1100)
                     /gene="lptD"
                     /locus_tag="VFT30_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEU5092025.1"
                     /translation="QGFTLNRIDVAPGISTDVIHLGHVIGIRPQAKFREVYYTRGVQS
                     DEGQHRETFWLGVDATSKMTRRFALADGNSLLHTVEPSLTYEYVPSSDQSKLTQIDQV
                     DDLPKKNLLTYMLRSRVLESGKKNAFNWLDLTLAQSYHVGDVQTLAREFTPGVAPFLG
                     SFTQPLQPATVPIQGRKFSDIWMRAVIGNNLPTLLTTAWLDTPVVGRAAQAWALRPPI
                     NRYLTVDAFFDPYRPGVSQFNTDLRFQEGTNWYFEVGQRYTRNGNRVRRGDLWNPISF
                     NEVFAPTEEIQFVTMGGAFRTPWGWTFGAKAYYDVKNGRSPELDAVALYQNPCRCWSV
                     GLYYLKFPDREQYSFMLSLTGVGWTESAGTVV"
BASE COUNT          255 a          320 c          277 g          248 t
ORIGIN      
        1 atgacgacag tcccggcgct ttcggtccac cctactccag tgagactcaa catgaagctg
       61 tattgctcac gatccgggaa tttcaagtaa tacagcccta cagaccaaca cctgcatggg
      121 ttctgataca aggcgactgc gtccaattcc ggacttctcc catttttgac gtcgtagtag
      181 gctttggctc caaaagtcca cccccaagga gtgcggaacg cgccgcccat ggtcacaaac
      241 tggatttcct cggtcggggc aaacacctcg ttaaatgaga tcgggttcca aaggtcgcct
      301 cgcctgacac gatttccatt tcgcgtgtac cgttgtccca cttcgaaata ccagttggtt
      361 ccctcttgaa atcgaaggtc cgtgttaaat tgacttaccc ctggtcgata gggatcgaag
      421 aatgcgtcga ccgtcagata ccggttaatc ggcggccgga gagcccacgc ctgcgccgcg
      481 cgtccgacca ccggagtatc caaccacgcc gtcgtcagca gtgtcggcag gttgttgccg
      541 atcacggccc gcatccagat atcagaaaac ttcctgcctt ggataggcac cgtggcgggt
      601 tggagcggct gggtgaatga tcctaggaaa ggggcgaccc ccggcgtgaa ctcccgggcc
      661 agagtctgca catcaccgac atgatagctc tgagccaacg tgagatcgag ccagttgaaa
      721 gcatttttct tgcctgattc cagcacgcga ctgcgcagca tataagtcag cagattcttc
      781 tttggcaggt catcgacctg atcgatttgc gttaacttcg attgatcact cgacgggaca
      841 tattcatacg tgagcgaggg ttcgacagta tgcaaaagac tgtttccatc agcgagtgcg
      901 aaccggcgag tcatcttcga tgtggcatcc acccccagcc aaaaagtttc tcgatgctgc
      961 ccttcatccg attgcacccc tctggtgtaa taaacctcac ggaacttcgc ttgcggtcga
     1021 atcccaatga catgcccgag atgaatcaca tcggtcgaga tacccggagc cacatcgatc
     1081 cgattaagcg taaatccttg
//
LOCUS       DASTWE010000476         1424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_594563, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000476 DASTWE010000000
VERSION     DASTWE010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594563"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..139
                     /locus_tag="VFT30_05030"
     CDS             17..139
                     /locus_tag="VFT30_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU5092026.1"
                     /translation="MIEAIICAAKTGSIGDGKIFVRELSEVVRIRTGETGETAL"
     gene            198..>1424
                     /gene="glnA"
                     /locus_tag="VFT30_05035"
     CDS             198..>1424
                     /gene="glnA"
                     /locus_tag="VFT30_05035"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU5092027.1"
                     /translation="MNVREVLEFAKKHRVQMVDLKFIDLPGVWQHMTIPVSELMEELF
                     KDGSGLDGSSIRGWKAINNSDLLVVPDPATACLDPFTAVPTLSLTGNVVDPISRENYE
                     RDPRFIAQKAEKYLQSTKIGDNSFWGPEAEFFIFDHARYDQTSHSGFYYIDSEEGVWN
                     MGQEGINLGGKIRHKQGYFPVAPADTQQDIRSEMVLEMEKVGIEVEKHHHETASAGQA
                     EIDIRFDSLLRTADKMMLYKYIVKNVARRHGKTVTFMPKPLFSDAGSGMHTHQSIWKD
                     GKPLFAGKDYAGISQLCLYYIGGILKHAPALAAFTNPTTNSYKRITPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPSCNPYLAFSAMLMAGLDGIQNKINPGEPA
                     EKDLYDLDPKEAASIPT"
BASE COUNT          352 a          349 c          418 g          305 t
ORIGIN      
        1 tgcccaggtg tctcgggtga ttgaggccat catctgcgcg gccaaaactg gtagtatcgg
       61 ggatggaaaa atttttgttc gagagttgag cgaggtcgtg cgcattcgga ctggagaaac
      121 cggagaaacc gcgctgtgac gatacaggat ccagtggtgg aacatgatcg cggaggatct
      181 tctaaaggag gatgggcatg aacgtgcgtg aggtattgga gttcgccaag aagcacaggg
      241 tccagatggt cgatctgaaa ttcatcgatc tgccgggtgt atggcaacat atgactatcc
      301 ctgtgagcga actgatggaa gagctgttca aagacgggtc cggcctcgat ggatcgtcga
      361 ttcgaggctg gaaggccatc aacaatagtg acttgttggt ggtaccggac cctgccacgg
      421 cgtgcctgga tcctttcacc gcggtgccga cgctcagctt gaccggaaac gttgtagacc
      481 cgatttctcg ggaaaattac gaacgtgatc ccaggtttat cgcgcaaaag gctgagaaat
      541 atctgcagag taccaagatc ggcgacaact cattttgggg accggaagcc gaatttttta
      601 tcttcgatca tgcccgttac gatcaaacca gtcatagcgg tttctactat atcgactctg
      661 aggaaggcgt atggaacatg gggcaagagg ggatcaacct gggggggaag atccggcata
      721 aacaaggata tttcccggtg gctccggccg atactcagca agatatccgg agtgagatgg
      781 tcctggaaat ggaaaaggtc ggcatcgagg tcgaaaagca tcatcacgaa acggcctcgg
      841 ccgggcaggc cgaaatcgat atccgatttg attccctctt gcggaccgcg gacaagatga
      901 tgctgtacaa gtacatcgtc aaaaacgtgg cgcgccgaca tggaaagacg gtgaccttca
      961 tgccgaaacc gcttttcagc gatgctgggt ccgggatgca tacgcaccag agcatctgga
     1021 aggatgggaa accgctcttt gccgggaaag actatgccgg gatctcgcag ctctgtttgt
     1081 attatatcgg tggcattctg aagcatgcgc cggcgctggc ggcttttacg aatccgacga
     1141 cgaattccta caagcgcatc acgccgggat tcgaggcacc ggtgctgttg gcctattcca
     1201 gtcggaaccg atcagccggt atccgtattc cgatgtactc cccaagtccg aaggcgaagc
     1261 ggatcgaagt acggtttccc gatccgtctt gtaatcccta tttggcattc tccgcgatgc
     1321 tcatggctgg gctcgacggc attcagaaca aaatcaaccc aggcgaaccg gccgaaaaag
     1381 acctttacga tctcgatccg aaagaggcgg cgagtattcc aacc
//
LOCUS       DASTWE010000477         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_336281, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000477 DASTWE010000000
VERSION     DASTWE010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336281"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2009)
                     /locus_tag="VFT30_05040"
     CDS             complement(<1..2009)
                     /locus_tag="VFT30_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEU5092028.1"
                     /translation="MDRNDGDRTSERDGLDIAVIGLACRFPGSHDSMAFWTNLRDGVE
                     SITALSDEDLRSSGVQERLRQDPNYVRMRGILDGIDLFDADFFGVTPKEAEMMDPQHR
                     LFLECAWETFEHAGYDPERFHGSIGVYAGSSTSGYLFNLFPQGVLLQSAADMAGLLGV
                     EKDSLPTRVSYKLDLEGPSIAVQTACSTSLVAVHLACQGLLAGECDMALAGGISVNVP
                     QKVGYLYQKSGIASPDGHCRAFDADARGTVGGSGVGIVLLKRLEEARVDGDHIVAVIK
                     GTAINNDGAHKVGYTAPRVEGQSKVIRAAHVAAGVDPGSIRYVEAHGTGTPMGDPIEV
                     TALTQAFRVGTDRNGFCAIGSVKTNIGHLDAAAGIAGLIKTALALSHQQIPPSLHFSS
                     PNPEIDFYETPFYVNTKLSDWTSHDSPRRAGVSSFGLGGTNAHVVMEEAPPVDREPHP
                     VERRPRLIVLSAKSETALNEMTTRFAAYLQQHPMAEPVDVAYTLQIGRKAFPHRRWAV
                     ADTIESVTRALSRPDSVKTSGQDGEPRPVVFLFSGQGAQYCTMGRGLYAHESIFRDEV
                     NRCAALLVSHIGMDIKSVLFEESATASERLNQTALTQPALFVLEYALAKLWMSLDVHP
                     QGMIGHSIGEYVAACLSGVFSLEDALQLVAARGRLMQSMPPGSMLA"
     gene            complement(2011..2682)
                     /locus_tag="VFT30_05045"
     CDS             complement(2011..2682)
                     /locus_tag="VFT30_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEU5092029.1"
                     /translation="MAECIQRIYQLTDPQRRLLLHRLGKSAGTRFGESRLVGCVVPRI
                     DTAATAQDLRSFLAERLPGYMIPTAWVFLDAFPLTSRGKVDRQTLKNHTRHIQKTESP
                     SAPLETDTERAIAEIWKEALGLQAVGRQDNFFDLGGHSLLLGKVLTKVRSLSKRPLGM
                     VDLFQYPTVQTLAAYVTGEGPLMEGRGAVAQVEQTQREKERRLAGSQRLKRQREQRRA
                     MTRGT"
     gene            complement(2684..>3436)
                     /locus_tag="VFT30_05050"
     CDS             complement(2684..>3436)
                     /locus_tag="VFT30_05050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5092030.1"
                     /translation="LNPPRKNQRNWLLRRALDEFSDDLVQLDDIAKRFVSGSDRVDIE
                     RTWAESQAEYTPTELIIEGQQVMQDWETPLMKAMADIATESGGEVLEVGFGMGISASF
                     IQERRPKSHTIIEFNQDVMVAFEQWRRRYLDRDIRLVRGRWQDVAETLSEFDSVFFDA
                     YPASEAEFEESVVNSITFAESFIPVAANLLRPGGVLTYYTNEIDSFSRRHQRFLFKHF
                     RSFTLSVVRSLRPPGNCHYWWADSMVVVKAVK"
BASE COUNT          727 a         1043 c          941 g          725 t
ORIGIN      
        1 gccagcattg aacccggcgg catactttgc atgagtcgtc cgcgagccgc gaccaattgc
       61 agcgcatctt cgagcgagaa gacaccggac agacacgcgg cgacatattc tccgatactg
      121 tgtccgatca tgccttgagg atgcacatcg agactcatcc aaagtttggc cagcgcatat
      181 tccaatacga acaaagccgg ttgcgtaagg gcagtttggt tgagtctctc cgaagccgtc
      241 gcggactcct caaacagaac cgacttgatg tccatgccga tgtgagaaac gagcagggcc
      301 gcgcatcggt tcacctcgtc gcggaatatc gattcatggg cataaaggcc gcggcccatc
      361 gtgcaatact gagcaccctg cccggagaat agaaacacca ccggtctcgg ctcgccgtcc
      421 tggccgctcg ttttcacgga atcgggccga ctcaacgcgc gcgtcacaga ttcgatggtg
      481 tcggccacgg cccatcggcg atggggaaat gctttccgac ctatctgcaa cgtataggca
      541 acatccactg gttcagccat cggatgttgc tgaagatagg cggcaaatcg ggtcgtcatc
      601 tcgttcagtg cggtttcgga tttagcggac agcacgataa gccgcggacg tctctcgaca
      661 gggtgaggct cccggtctac cggcggtgct tcctccatga cgacatgcgc attggttccg
      721 ccgaggccga acgagctgac accggcacgc cgaggactgt cgtgggatgt ccagtcggac
      781 agtttcgtat tgacgtaaaa aggcgtctcg taaaaatcaa tttcaggatt cggggaagaa
      841 aagtgcaggc tgggaggaat ctgttggtgc gacagggcga gggctgtctt gatcagcccc
      901 gcgatccctg ccgctgcatc gagatgtccg atgttggtct tgaccgatcc gatggcacag
      961 aagccgtttc ggtccgtgcc gacacgaaag gcctgggtga gggccgtcac ttctatcggg
     1021 tcgcccatcg gcgtcccagt accatgcgct tcgacatatc gtatcgagcc cgggtcaacc
     1081 ccggccgcca cgtgcgccgc ccggatcacc ttggactgtc cttcgacgcg cggggcggtg
     1141 tacccgacct tatgcgctcc atcgttattg atcgcggtgc ctttgatcac cgccacgata
     1201 tgatctccgt cgactcgtgc ctcttccaac cgtttcaaca gcacgattcc cactccgctt
     1261 cctccgaccg ttccgcgcgc atccgcgtcg aatgcccgac aatgcccatc gggagaggca
     1321 atcccgctct tctgatagag atagccgacc ttttgcggca cgttcacgga aatgcccccc
     1381 gccagcgcca tgtcgcactc accggcgagc aggccctgac aggccaggtg tacggcaacc
     1441 agagatgtgg aacaggcggt ttggacggcg atgcttggtc cttccagatc caatttgtag
     1501 gacacgcgag tgggaagcga atccttctcc acgccaagca gtcccgccat atcggcagcc
     1561 gattgcaaga gcacgccctg agggaaaaga ttgaacagat agccgctggt acttgagccc
     1621 gcgtagacac cgatcgatcc gtgaaaccgt tccgggtcat agcccgcatg ttcgaacgtc
     1681 tcccacgcac attcgagaaa cagccggtgc tgcggatcca tcatctcggc ttctttggga
     1741 gtcacgccga aaaaatccgc atcgaagaga tcgatcccgt cgaggatgcc ccgcatgcgg
     1801 acatagttcg gatcctgacg cagacgttct tgaactccgg atgatcgcaa atcctcatca
     1861 ctgagagccg taatggattc cactccatcc cgcagattgg tccagaatgc catgctgtcg
     1921 tgactgccgg ggaaccgaca ggccagcccg atcacggcaa tgtccaatcc gtcgcgctcg
     1981 ctggttcgat caccgtcgtt tcgatccatc tcaggttcca cgcgtcatcg cccgtcgttg
     2041 ttcccgctgc cgcttcaacc tttgtgagcc tgctaggcgt cgttcctttt cccgttgcgt
     2101 ctgttccact tgagccacag ctccacgacc ttccatcaga ggtccttcac ccgtgacata
     2161 tgctgccagg gtctgcacgg taggatattg aaacaaatcc accattccta atggccgttt
     2221 gctcaatgag cgaaccttcg tcagcacctt accgaggaga agtgaatgcc ctccgagatc
     2281 gaaaaaattg tcctgacgac cgactgcttg gaggccgagc gcttccttcc aaatttccgc
     2341 gatagcccgc tcggtatcag tttcaagcgg cgccgacggc gattccgtct tctgaatatg
     2401 tctggtatgg ttcttcagag tctgacgatc taccttccca cgcgaggtga gcggaaaggc
     2461 gtcgaggaat acccatgcgg tcggaatcat gtagccgggc agccgctctg ccaggaaact
     2521 ccgaagatcc tgtgcagtcg cggcggtgtc gatccgaggg acgacacagc ccaccaatcg
     2581 tgattcccca aatcgagtac ccgccgactt ccccaggcgg tggagaagca gccgccgctg
     2641 cgggtcggtc aattgataga ttcgttggat acactccgcc atttcatttc accgctttta
     2701 cgacgaccat cgagtccgcc caccaatagt ggcagttccc aggcggtcgc agcgatcgca
     2761 cgacactgag cgtgaacgaa cggaaatgct tgaagaggaa tcgttggtgt cgccgactga
     2821 aggaatcgat ctcattcgtg tagtaggtca agacaccgcc gggtcggaga aggtttgccg
     2881 ccacgggaat gaatgattcg gcgaaggtga tgctgttgac gaccgattcc tcgaactccg
     2941 cttcactggc agggtacgca tcgaagaaga cactgtcgaa ttccgaaagc gtttccgcaa
     3001 cgtcctgcca acgacctcgt accaaccgga tgtcacgatc gagataccga cgtcgccatt
     3061 gttcgaaggc gaccatgacg tcttgattga actcgatgat cgtatgcgac ttaggacgcc
     3121 tttcttgaat gaaggaggcc gagattccca ttccaaatcc gacctcgagc acctccccgc
     3181 ctgactctgt cgcgatgtcg gccatcgcct tcatcaacgg cgtttcccaa tcctgcatga
     3241 cctgctgtcc ctcgatgatc aactccgtcg gcgtgtattc cgcctggctt tccgcccagg
     3301 tccgttcgat atcgacgcga tccgagcctg aaacgaagcg tttggcgatg tcgtcgagtt
     3361 ggaccagatc atccgagaat tcgtcgagcg cacggcgcaa aagccaattg cgttgattct
     3421 tccgaggagg gttcag
//
LOCUS       DASTWE010000478         1344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_788618, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000478 DASTWE010000000
VERSION     DASTWE010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788618"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFT30_05055"
     CDS             <1..207
                     /locus_tag="VFT30_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092031.1"
                     /translation="KKANRMDRAAQETALKESGGVQPYVIWVGVFFYIIFLATLVKMI
                     QEGPLDLDKSPGRPAKQTVLLSQA"
     gene            274..879
                     /locus_tag="VFT30_05060"
     CDS             274..879
                     /locus_tag="VFT30_05060"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEU5092032.1"
                     /translation="MARLVLLRHGESQWNLENRFTGWVDVPLSAKGVEEARQAGEKLR
                     GFTFDRAFSSVLIRANETLRIVLEVLGQTNIPIEKDKALNERMYGDLQGLNKAETAKK
                     YGDAQVKIWRRSYDVKPPGGESLKDTAERALPYYEKMVKPHLLKGETIIVAAHGNSLR
                     ALVMELEQLSKEEVLELNIPTGVPLLYEFDDSGKVLSHRYL"
BASE COUNT          340 a          321 c          377 g          306 t
ORIGIN      
        1 aaaaaagcca accggatgga tcgtgccgca caagaaaccg cgctgaaaga atcgggcggg
       61 gtgcagcctt atgtcatctg ggtcggcgtg tttttctata ttatcttcct ggcgacactg
      121 gtgaagatga ttcaagaagg cccgctcgat ctggataaat cgcccggtcg accggcgaag
      181 caaacggtcc tgctgtcaca ggcttgactt ggcacggcaa ttcagtatgc tttgatccct
      241 gatgatgctc acattcacgg taaaggatgc gtgatggctc gattagtctt gcttcgtcat
      301 ggtgaatcgc aatggaattt ggagaaccgt tttaccgggt gggtggatgt cccgctttcc
      361 gcaaaaggtg tcgaagaagc gaggcaagcc ggtgaaaagc tccgcgggtt tacctttgac
      421 cgagccttct cctctgttct catccgcgcc aatgaaacgt tgagaattgt gctggaagtt
      481 ctcgggcaaa cgaatattcc cattgaaaaa gacaaggcgc tgaacgaacg gatgtatgga
      541 gacctccaag gactcaataa ggccgaaacc gccaagaagt acggtgacgc gcaagtgaaa
      601 atctggcgac gaagctatga tgtgaaaccg cctggcggag agagtcttaa agacacggct
      661 gagcgcgcgt tgccctatta tgagaaaatg gtcaaacccc atttattgaa gggggaaacc
      721 atcatcgtgg cggcgcatgg caacagtctt cgggcgctgg tgatggaatt ggagcaattg
      781 tcgaaggaag aggtcttgga gctcaacatt ccgaccggcg taccgctgct ctacgaattt
      841 gacgacagcg gaaaagtatt gtcacaccgg tatctgtgag cgtagcgctt gtccaacagt
      901 tctgattgat aacttactcc acgtatcgac ccccagccgg acggacgtcc atcacttctt
      961 ctggatcgac tcgcataaca catccgtgat cgtagaccag cacttaggga tattggcatc
     1021 gattgcggcc aagagccaat ggagctcagc gacaaaatct cccgcgccat tcgctgggcg
     1081 gtggagtcag aggcctcgtg ttgagagacg gtgatgggtt gcggctttga agaacactgc
     1141 tgcagcgtcg gtagggattc atagatccct cgtgtgcgtt acagaatacg gaagacctgt
     1201 ctcactctca agtggtggtc tggatgagaa catagtcacc atacgtctct ccgcaaaccg
     1261 cggatgacgt gaacccgcaa gtaaatgcta catcatgtgc agcatgattt tatgatttca
     1321 cagagtgaaa ggatttgtat gctg
//
LOCUS       DASTWE010000479         1414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_303731, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000479 DASTWE010000000
VERSION     DASTWE010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303731"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFT30_05065"
     CDS             complement(<1..1383)
                     /locus_tag="VFT30_05065"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEU5092033.1"
                     /translation="MYVVDSGNNRILKYVPTEEEIDRGKDQPTQTVEAGAVQPPRSLA
                     VKAGDTEVFLSWMEVSGAQSYNLYFSTTPHVTLEGATKIEGVTNPYTHEGLTNDTPYF
                     YAATALFEDGTESGLSEEVTATPVLIDITAPQNPYAVINHGAFMTNSPDVVVTISATD
                     LDSGVGAYFISESPLTPMAGTPGWVDATPAIKFGATIPFILSPGDGHKTIYVWFKDIG
                     NNVSTPASTTILVNTSGYLCVSKWGKPGRGASLLHGGEFMAPIYGLCVDQQGSLFVVD
                     NGNNRVQKFDNAGNFIILWGSFGSAGANFHNPTGIACDGKGDVWVVDTNNHRVQKFDG
                     KLGGYLMKFGSRGNGEGQFNAPWGIAVDRVRGYLYVVDSANFRVQKFDMSGEFIMAWG
                     SFGNGDGQFYFPRGVAVDQEDGSVYVVDMGNHRIQKFDTSTNVLPQLLTKWGGSPIAG
                     HASSSLAQEAG"
BASE COUNT          295 a          429 c          372 g          318 t
ORIGIN      
        1 cccggcctcc tgtgccaatg aactactggc atgcccggct ataggactgc caccccactt
       61 cgtcaatagc tgcggcaaga cattggtgct ggtatcgaat ttctggatgc ggtgatttcc
      121 catgtcgacg acataaaccg acccatcctc ttgatccacg gccactccgc gtgggaaata
      181 gaactgcccg tccccgttgc cgaagcttcc ccaggccatg atgaactcac cggacatgtc
      241 gaatttctgc acacggaaat tggcgctgtc gacgacgtag agatagccgc gcacacgatc
      301 gaccgcaatt ccccagggcg cattaaactg tccttcaccg tttccacgcg aaccgaactt
      361 catgaggtag ccgccgagct tcccatcgaa cttttgaacc cggtggttgt tggtatccac
      421 cacccagaca tcgcccttgc cgtcgcaggc gatgccggtg ggattgtgaa aattcgcgcc
      481 ggctgagccg aaacttcccc acagaatgat gaagttcccg gcattgtcga acttctgcac
      541 gcgattgttg ccgttatcga cgacaaacag cgatccttgc tgatcgacgc acagtccgta
      601 tatcggcgcc ataaactctc cgccgtgcaa cagcgacgca cctcggccgg gcttccccca
      661 tttcgacacg cagagatagc ccgatgtatt gaccaaaatt gtggtgctgg ccggcgtgga
      721 gacattgttg ccgatgtctt tgaaccacac ataaatggtt ttgtgcccgt ctccagggga
      781 gagaatgaac ggaatagtgg ctccgaactt gatcgccggt gtcgcatcca cccaccctgg
      841 tgtcccagcc atcggggtaa gcggactttc agaaatgaaa taggccccca cccccgaatc
      901 taaatcggtc gccgaaatcg tcaccacgac atccggcgaa ttggtcatga aggccccatg
      961 attgatcacc gcgtagggat tctgaggcgc cgtgatatcg atgagtacgg gcgtcgccgt
     1021 cacttcttcc gagagtccgc tttccgtccc atcttcgaac aacgcagtcg cggcataaaa
     1081 atagggcgta tcgttggtga gaccttcatg tgtgtagggg ttcgtcacgc cttcgatttt
     1141 cgtcgccccc tcaagcgtga catggggcgt ggtgctgaaa tagagattgt aggattgcgc
     1201 gccggatacc tccatccagc tcaggaagac ttccgtgtca ccggccttga cggcgaggct
     1261 gcggggtggc tggaccgccc ccgcttccac cgtttgggtc ggctggtcct tcccacgatc
     1321 tatctcttct tcggtgggta catatttgag gatacggttg ttgccactgt cgacgacgta
     1381 cacacaccct tccttatcca ctgcgatgcc gtag
//
LOCUS       DASTWE010000480         1206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_594642, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000480 DASTWE010000000
VERSION     DASTWE010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594642"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFT30_05070"
     CDS             <1..302
                     /locus_tag="VFT30_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092034.1"
                     /translation="ADIVISGQVQDFSATVKSRVFSTVIDTSSRFTIQARNLVDRSTT
                     IRTIEGGRSRTVFWFNQDDVRDQLAATLKDGLDRLIVDTTIDNRALRPSRRLLQE"
     gene            386..>1206
                     /locus_tag="VFT30_05075"
     CDS             386..>1206
                     /locus_tag="VFT30_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092035.1"
                     /translation="MRERRWETTTPLNFGQALAVGDRLAALGLKPVSPASDVICYVEE
                     WTVGSLDDFDQLDPWSTEDVTLVHVRERWRGDFFLLSGAYHTIYQRHQDIGTYCSISH
                     PWRIREILQLHDQRAMFWIGFRHAHSFIRVRLQTQVVITPGETRGDADRTRWLDERRA
                     AFLDAITVLDLPIETHVEKNVVIVRPSDASIPFFCSWPDAFGPCQFEYNTSDAFEFLV
                     PASKLAETFHPEPAGVRAYLTGFSEAALTEFQGIEPGARLAYRCSVHCPLDDLPEV"
BASE COUNT          241 a          346 c          343 g          276 t
ORIGIN      
        1 atgccgatat tgtcatcagc ggacaggtgc aagatttttc cgctaccgtg aagagccgcg
       61 tcttttccac tgtgattgac accagcagca ggtttacaat tcaggccagg aacctcgtcg
      121 accgaagcac gaccattcgc accattgaag gggggcggag tcgaaccgta ttttggttca
      181 accaggatga tgtgcgagac cagttggcgg cgaccttgaa agacggactt gatcggctga
      241 tcgtcgatac gacgattgac aacagggcat tacgcccgtc acggcgtctc ctgcaagagt
      301 gaccgttgca ctccccgttg ggaaagaggc attgattttc tgagggtcga cggcaacttc
      361 aatctgtctt caaggatccc tcacagtgcg tgagcggcgg tgggaaacga cgacgccctt
      421 gaatttcggt caggcgcttg ccgtggggga ccgactcgcc gccctcgggt tgaagccagt
      481 ctcgcccgcc agcgacgtga tctgctatgt agaggaatgg acggtcgggt ccctcgacga
      541 cttcgatcag ctcgatccct ggtccacgga agacgtcacg ctcgtccacg tacgggaacg
      601 atggcgcggc gatttctttc ttctgtccgg agcctatcat acgatctatc agcgtcatca
      661 agatatcggc acgtattgct ccatcagcca tccgtggcgc attcgagaaa tactgcagct
      721 tcatgatcaa cgtgccatgt tctggattgg gtttcggcat gcccattcgt tcatccgcgt
      781 tcgcctccag acccaggtcg tcatcacgcc cggcgaaacg cgtggcgatg ccgatcggac
      841 acgctggttg gatgagagga gagcagcctt cctcgacgca attaccgttt tagacctgcc
      901 catcgagacc catgtggaga agaacgtggt gatcgttcga ccttccgatg cttccattcc
      961 gttcttctgc tcatggcccg atgcgttcgg tccttgccag ttcgagtaca acacgtccga
     1021 tgcgtttgaa ttcctcgtgc cagccagcaa gcttgcggag acgttccatc cagagccagc
     1081 cggagttcgt gcctatctca cagggttttc agaagcagcc ttgacggaat tccaggggat
     1141 cgagccggga gcgcggcttg cctatcgctg ctccgtccat tgcccgttgg atgatctgcc
     1201 ggaggt
//
LOCUS       DASTWE010000481         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1241088, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000481 DASTWE010000000
VERSION     DASTWE010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241088"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>1672
                     /gene="ligA"
                     /locus_tag="VFT30_05080"
     CDS             122..>1672
                     /gene="ligA"
                     /locus_tag="VFT30_05080"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5092036.1"
                     /translation="MRQDSLYGDSPSSSRATPSEQERLAQLKSEIRHHDYLYYVKDRP
                     EISDGEYDRLFRELADLEQAYPELVTPDSPTQRVGASPRGELGKVQHERPMLSLDSLV
                     NSEEVLAFDQRMKRELGTDQVEYTVEPKFDGLSVEMVYDHGTFVRGSTRGDGNVGEDI
                     TINLRTIRSLPLQLQAGTHPDHLVVRGEVYMRLSDFHALNRRMTERGGDVFANPRNAA
                     AGSLRQLDSKITASRPLVVICYEVMTMTGAPPPSHWAELETLAMCGLPVPSIRRRCET
                     IEQVLAFHRETEEQRDNLPYEIDGVVVKVDRGDWQARLGMKSRSPRWAIAFKFSPRKE
                     ITEVQDIAISVGRTGTLTPLALLKPVEVGGVTISRATLHNADEVARKDIRIGDTVRVE
                     RAGDVIPAIAERIPVSNEVRSDPFQMPHHCPICGSAVAREGAYFYCTGQAVCPAQLKG
                     AIEHFASKSALNIEGLGKKTVAQLVDQGLVKDLSDLYRLDREQLLKLQGFAERSATLL
                     LDAIARSKS"
BASE COUNT          387 a          474 c          452 g          359 t
ORIGIN      
        1 cgttccgcgg ccctatgcta gggtcagcac ggcttaggcc cctctcccat ttcatgccga
       61 acgtcatccg tcatggcccc ttagagacga tcgttgagac gtgatctcgt tgagagcagc
      121 gatgcgacaa gattctctct atggcgactc tccctcctct tcaagggcca ctccctccga
      181 gcaagagcga ctcgctcaac tcaaaagcga aatcaggcat catgactacc tctattacgt
      241 caaggaccgc cccgagattt ccgatggaga gtatgatcgg ttattccgag aattggccga
      301 cctcgaacag gcatacccgg agctagtcac ccccgattcc cccacccagc gcgtcggtgc
      361 ctcacctcgg ggcgaactcg gcaaggtaca gcacgaacgg cccatgttga gccttgattc
      421 gttggtgaat tctgaggagg tcctggcctt cgaccagcgg atgaaacggg agctgggcac
      481 ggatcaagtc gaatacacgg tcgagccaaa atttgacggc ctatcagttg aaatggtcta
      541 cgaccacgga acgtttgttc gtggttcaac ccggggtgat ggaaatgtcg gagaggatat
      601 caccattaat ctccgcacga ttcgatcgtt accgctacag ttgcaggccg gcactcaccc
      661 agatcatctg gtcgtgcggg gagaagtgta tatgcgtctc tccgactttc acgcgctcaa
      721 ccgccgaatg acagaacggg gaggcgacgt ctttgcaaac ccgcgcaacg ctgccgccgg
      781 ctctcttcgg caactcgatt cgaaaattac cgcctctcgg ccgcttgtcg tcatttgcta
      841 tgaagtgatg acgatgacgg gagcccctcc cccatcgcac tgggctgaac ttgagacgtt
      901 ggctatgtgt ggacttccgg tccctagtat tcgtcgacga tgcgaaacca tcgagcaggt
      961 cttggcattt caccgggaaa cggaggaaca gcgcgataat cttccctatg aaattgatgg
     1021 agtagtggtg aaagtcgatc gcggagattg gcaagcgcga ctgggaatga aatccagaag
     1081 tcctcgttgg gccatcgcgt tcaagttttc tcctcggaag gaaattacgg aagtacagga
     1141 tattgccatc tccgtcggcc gaaccggaac cttgacccct ctcgccctgc tgaagccggt
     1201 ggaagtgggt ggggtcacca tcagccgagc aaccctgcac aatgccgacg aggtggcaag
     1261 aaaggacatc cgtataggag acacggttcg agtcgagcgg gcaggagatg ttattccagc
     1321 aatcgctgaa cgtatccccg tctcgaatga agtcaggtcg gaccccttcc aaatgcccca
     1381 tcactgtccg atatgcggtt ctgccgtggc aagagaaggt gcctactttt actgcacagg
     1441 ccaagccgtc tgtcctgctc agctgaaagg cgcgatcgag cacttcgcgt caaaaagcgc
     1501 actgaatatt gaaggcctcg gcaaaaagac agtcgctcaa ttagtcgatc aaggactcgt
     1561 caaggatctt tctgatcttt atcgccttga tcgggagcaa ctcttaaagc tccaagggtt
     1621 tgcggagaga tccgcaaccc tgctccttga cgccatcgct cgaagtaaat cg
//
LOCUS       DASTWE010000482         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1241090, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000482 DASTWE010000000
VERSION     DASTWE010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241090"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1132
                     /locus_tag="VFT30_05085"
     CDS             <1..>1132
                     /locus_tag="VFT30_05085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5092037.1"
                     /translation="RLGVVGVVVADESALSYRNIPNVLSAAEITGVDAIHPGYGFLSE
                     NAHFAEVCESIGIKFIGPSSENIAMMGDKAKAREIVAKRGLPVTPGSPGELRSEEDAL
                     QAAQKIGFPVIIKATAGGGGRGMRVVNKADDLGRAFQAAQAEAKSTFGNDGVYLERYF
                     LEPRHIEVQILADQYGRVVHLGERDCSIQRRHQKLVEETPSPVVDDKLRREIGRVAVE
                     AVKAAHYRNVGTVEFLLDKDRQFYFMEVNTRIQVEHPITEMVTGIDLIKEQIRLAAGH
                     PLSIRQQDVTLTGHSLECRINAEDPEKFTPSPGTITKYSPPGGFGIRVDSAMESGSVV
                     VPYYDSMIAKLITHGRDRQESVARMKRALGEFIIEGIKTTIPL"
BASE COUNT          264 a          284 c          340 g          244 t
ORIGIN      
        1 accggctcgg tgtcgtcggg gtagtcgtag ccgacgaatc ggcattgagt taccgaaata
       61 ttcccaacgt cttgagtgcc gcggagatca cgggagtcga cgccatccat cctgggtatg
      121 gatttctgtc ggagaacgcc cattttgcgg aagtctgtga gtcaatcggc attaagttca
      181 tcggaccgag ctcggaaaat atcgccatga tgggggataa ggcgaaggcg cgtgagatcg
      241 tggcgaagcg aggccttccg gtcacaccag ggagtccagg tgaattgcgc agcgaggagg
      301 atgcgttgca ggcggcgcag aagatcggtt ttcccgtcat tatcaaagct acggctggag
      361 ggggagggcg aggcatgcgt gtcgtcaaca aggccgatga cctaggccga gcctttcagg
      421 ctgctcaggc tgaagcgaaa tcgaccttcg gcaacgacgg cgtgtacctg gagcgatact
      481 ttctagaacc gcgtcatatc gaggtgcaga ttttggccga tcaatacggt cgggtggtcc
      541 accttggaga acgagactgt tcaattcaac ggcggcatca aaaacttgtc gaggaaactc
      601 catctccggt agtcgatgac aaattgcgtc gggaaatcgg tcgggtagct gtggaggcgg
      661 tgaaggctgc gcattaccga aatgtaggca ccgtggagtt tttgctcgac aaagaccgcc
      721 agttttattt catggaagtc aatactcgca tccaggtgga acaccccatc actgaaatgg
      781 tgaccggcat agacctgatt aaggaacaga tccgtctggc cgccggtcat cccctttcta
      841 tccggcaaca ggatgtgact ctcactgggc acagcctgga gtgtcgtatt aatgccgaag
      901 atccggaaaa gtttacgccg tctccaggaa cgatcaccaa gtacagcccg ccgggagggt
      961 tcggaatccg cgtcgactcc gccatggagt caggttcggt cgtcgttcct tactatgatt
     1021 cgatgattgc aaagctgatc acccatggcc gtgatcgaca ggagtcagtg gctcgtatga
     1081 aacgcgcact cggtgagttt atcatcgagg gtatcaagac aaccatccct ct
//
LOCUS       DASTWE010000483         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1144233, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000483 DASTWE010000000
VERSION     DASTWE010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144233"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VFT30_05090"
     CDS             complement(<1..1533)
                     /locus_tag="VFT30_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5092038.1"
                     /translation="MTDYGVLGNLLLIYTVSIAVVFLFHQFRLPSIAGFLVAGALIGP
                     HGLNLISDIATVQVLAEIGIVLLLFTIGIEFSLRQLTSLRRLLLIAAPIQVGGVIVIA
                     WLGGTLAGLPTPQAIFWGFLLSLSSTAIVLKALAASGDSDSPHGRAIIGILIFQDLAV
                     VPMILLTPILASHGEGTLTPVLFSLAKSIAVVACIVAAAWYVVPKLLDHIVRSRSREL
                     FLLTIIVMCLGIAWLTSLGGLSLALGAFIAGLVISESEYSHQAIAEVLPFRDSFNSLF
                     FVSIGILMDWHILLEYPLAVTGVLLVVLLVKFVAGAGAVLTISVPPRSAVMTGIALAQ
                     VGEFSFILAQVGLENKLLSGPPYQIFLAVSVCSMIITPFLMQLSPHLARRVEAVQRLH
                     HWFPGQTTAHVLEAEGRHLRIKDHVIIVGYGLNGRNLARVLGETEVPYIALDLDGDIV
                     SREAAHGLPLYYGDATNPNVLRHVKIEEARVLVVAISDPFMARRTVQVARGLNPKIHI
                     VVR"
BASE COUNT          511 a          542 c          574 g          402 t
ORIGIN      
        1 ccgtaccacg atgtgaattt ttggattcaa gcctcgagcg acttgcacgg ttcgccgggc
       61 catgaacgga tcggagatcg ccacgaccaa gacccgtgcc tcttcgatct tcacatgtcg
      121 taatacattc ggatttgtgg catccccata gtagagcggc aaaccgtggg ccgcttctcg
      181 actcacaatg tccccgtcca aatccaacgc gatgtagggc acttccgttt cacccaatac
      241 acgggccagg ttccgtccgt tgagcccata tcccacgata atcacatggt ccttgatgcg
      301 cagatgccgc ccttctgctt cgagcacatg ggccgtcgtt tgtccaggaa accaatggtg
      361 aagtcgttgc acggcctcaa cgcgtcgggc gagatggggg gataactgca tcaagaacgg
      421 cgtgatgatc atggagcaga ccgagactgc tagaaatatt tggtatggcg gtccggacaa
      481 aagcttattt tccaggccga cctgtgccag gataaaactg aactcgccta cctgcgcgag
      541 ggcaatccca gtcatgacgg cggagcgagg aggcacggag atcgtcaaga cggcgcccgc
      601 accggcgacg aactttacga gcagtacgac gagtaacacg ccggtcacgg cgagcggata
      661 ctctagcagg atgtgccaat ccatcaggat gccgatggag acgaaaaaca ggctattgaa
      721 gctgtctcga aacggtaaca cttcggcgat ggcttgatgg ctgtattcgg attcggaaat
      781 cacgagtccg gcgatgaagg ctcccaaagc gagcgacagc ccgccgagag atgtcagcca
      841 agcaatgccg aggcacatga caatgatggt caatagaaag agttctcggc ttcgactacg
      901 aacgatgtgg tcgagtaatt tcggaaccac ataccacgcg gctgcgacaa tacatgcgac
      961 tactgctatc gatttggcca acgagaacag cactggtgtg agcgtccctt cgccatggct
     1021 ggcaagaata ggcgtcaaca agatcatagg aacgacggcc aagtcctgga aaatcaagat
     1081 cccgatgatg gctcgcccgt ggggtgaatc gctgtctcca ctggctgcca gcgctttcag
     1141 tacgatggcg gtgctgctga gcgagagcag gaacccccaa aagatcgcct gaggtgtcgg
     1201 taatcctgca agagtcccgc cgagccatgc gatcacgatg actcccccga cttgaatcgg
     1261 agcagcaatt aagagcagcc gacgaagcga ggtgagttgc ctcagagaga attcgatacc
     1321 gatggtaaac agaagcagca caatcccgat ttccgccagt acttgcaccg tcgcgatatc
     1381 agaaatcaga ttgagcccgt gaggaccgat caaagccccc gcgacgagaa acccggcgat
     1441 agacggcagt cggaattgat gaaacaaaaa caccactgcg atggagacgg tataaatcag
     1501 cagcaaattg ccgagcacgc cgtagtcagt catgtcgcgt cttcagcccg tcccttgttg
     1561 gtgatgcgtc ctgtggatgt caccctttgg tagccgacgg cgttcttcca tcgtagtcac
     1621 aggatcattg gtgaccgaaa atgagatacg caggatacct gaggtctatg gcagggacaa
     1681 ggtaaaggaa gatgtcggcc gggcgtttct gcacagcatc cagcgtattg tccatgctgc
     1741 aagtctcggt cggagccagc atggtggtat cgaaaacgaa ggggggagaa gtatgaggaa
     1801 ggtcagttaa tcggtgatcg ccaatagttt ctaagccgct ccagcattgg ccttcccctc
     1861 caaaggggca tagaccaaag aaccaagcat tgcgtagggt gtgaacacag ttcacaggac
     1921 tgatcacgag tgtccaggca ctgagtgcct tcgttacacg atctggaggt accccatgtg
     1981 gccgacgcgg atgtttcctt tcttagctgc aatgctggtg gccctttcc
//
LOCUS       DASTWE010000484         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_303907, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000484 DASTWE010000000
VERSION     DASTWE010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303907"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="prcB"
                     /locus_tag="VFT30_05095"
     CDS             complement(<1..827)
                     /gene="prcB"
                     /locus_tag="VFT30_05095"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEU5092039.1"
                     /translation="MTNGGSKTAMKLPYLPDHDGSSFFDFVSTHHPELAFRSHGMAAG
                     PDQIRTIGPVAVPSATTVLALKYQQGVVIAGDRRATEGFQIADRRIEKVFKIDDWSAM
                     AIAGAAGPCIEMAKLFQTELEHYEKLEGMPLSCEGKANKLGQMVKANLPMVFQGLVVM
                     PLYVGYDLKRGEGRIFKYDITGGRYEESDYHAIGSGGKDARNTMREHFQRNLSEGDAI
                     KLALLALYNAADDDVGTGGPDLVRGIYPTAKIVTAQGITDISDDTLRAVCNDVMAARR
                     "
     gene            complement(824..2296)
                     /gene="dop"
                     /locus_tag="VFT30_05100"
     CDS             complement(824..2296)
                     /gene="dop"
                     /locus_tag="VFT30_05100"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEU5092040.1"
                     /translation="MRVLGTETEFGIAAKDPSAMDPVSGSFAVIGHYPGLPAPEALWD
                     YENENPLLDARGFEVDGERERPNPDYNRQLNKVLANGGRLYVDGAHPEYSTPECSNAR
                     EVVAFERAGELILATCLQEMARVRGVEQYVLYKNNSDGKGNSYGYHENYLVSRSVSFD
                     RIARVLTPFLVTRPIFAGAGKVGSENQTSPTEYQISQRADFFECLMDLNTMVKRPIIN
                     TRDEPHADAARFRRLHVITGDANMAEFSTYLKVGTLDIVLDLLESGADLPQFELDEPV
                     RAFNQVSRDLDVKETVKLAGGRPTTALAVQRAYLSAAKTFYASQDHSPVTHDVLLRWE
                     DVLNQLERDPRLLENALDWIAKRHMIESYMERKGCGWDDPRMKLMDLQYHDVRPDKGL
                     FYTLERSRLIERIVEEGDIQRAELNPPTGTRAYFRGRCVSKFAKALYGASWTSVLFDV
                     GNTTIKKVPLMDPHRGTQALTRELLDTTDSVDALIEKLKV"
     gene            complement(2331..>2546)
                     /locus_tag="VFT30_05105"
     CDS             complement(2331..>2546)
                     /locus_tag="VFT30_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEU5092041.1"
                     /translation="AQEGTGLRSDDLIRAIREEFKEHEDLPNTTNPDDWAKVAGKKGE
                     KIIHIRTISGGPTEHRQIETISTGHYL"
BASE COUNT          528 a          749 c          672 g          597 t
ORIGIN      
        1 cgacgtgccg ccatcacatc gttgcacacg gcacgcagcg tgtcgtcgga tatgtcggtg
       61 atgccttggg cagtcacgat ttttgcggtg gggtaaatgc cccgcacaag atccggtccg
      121 ccggtcccaa cgtcgtcgtc cgcggcatta tagagtgcca acaaggctaa ttttatcgcg
      181 tccccctcgg agagattccg ctgaaaatgt tcgcgcatcg tgttgcgggc atctttccca
      241 ccggaaccga tcgcgtggta atcggattct tcatacctgc cgccggtgat gtcgtacttg
      301 aagatgcggc cctcgccacg tttcaagtca tacccgacgt acaacggcat gacgaccagc
      361 ccctggaaca ccatcggaag attggccttg accatctgac ccagcttatt cgccttccct
      421 tcacaggaaa gtggcatgcc ttccagtttt tcgtaatgct ccagctcggt ctgaaataac
      481 ttggccatct cgatgcaagg accggccgcc cccgcgatcg ccatggccga ccagtcgtcg
      541 atcttgaaga ctttttcaat tcggcgatcc gcgatctgaa acccttccgt tgctcggcgg
      601 tctccggcaa tcacgacccc ttgctgatac ttgagcgcca gcacggttgt agcagaaggc
      661 acagcgaccg gcccaatggt gcggatctgg tcaggacccg ccgccattcc gtggcttcgg
      721 aatgccaatt caggatgatg tgttgagacg aagtcaaaaa aactggaacc gtcgtggtcg
      781 ggaagatagg gcagcttcat cgcggtcttc gaaccaccgt tcgtcaaacc ttgagtttct
      841 cgatcagggc atcgactgaa tcggtcgtat cgagtagctc tcgtgtcaag gcctgcgtcc
      901 ctcgatgggg gtccatcaga ggcactttct tgatcgtcgt attgccgaca tcgaacagca
      961 ccgaggtcca actggcccca tacaacgctt tcgcaaactt actgacacaa cgcccacgaa
     1021 aataagctct ggtacccgtc ggcggattaa gctccgctcg ttgaatatcg ccttcctcga
     1081 cgatccgctc gataagacga ctgcgctcca gcgtatagaa caaccccttg tccggacgga
     1141 catcatgata ttggagatcc atcagtttca ttcgagggtc atcccatccg cagcctttac
     1201 gctccatgta ggactcgatc atatgtcgct tggcgatcca atccaatgcg ttctccaaca
     1261 gccgcggatc acgctcgagt tggttcaaaa catcctccca acggagcaac acatcgtgag
     1321 taacgggact gtgatcctgc gaagcataaa aagtcttggc ggcgctcagg tacgcccgct
     1381 ggacagcaag agctgtggtg ggccttccac cggccagttt cacggtttct ttcacatcca
     1441 ggtcgcggga cacctgattg aaggcacgaa ccggctcgtc cagttcgaac tgaggcagat
     1501 cggctcctga ctcaagcaaa tccaagacga tgtccaacgt tcccaccttc agataggtgg
     1561 agaattccgc catgttggcg tcgccggtaa tgacatggag cctccgaaac cttgccgcat
     1621 cggcatgcgg ttcgtcgcgc gtgttgatga tcggccgttt gaccatcgtg ttgaggtcca
     1681 tgaggcattc gaagaaatcg gcgcgttgcg agatttgata ttccgtcggg ctggtctgat
     1741 tttccgaccc aacttttcct gctccggcga agatcggtcg tgtgaccagg aacggcgtca
     1801 atacccgagc gatcctgtcg aacgacacgg acctcgaaac taaatagttt tcgtgatatc
     1861 cgtagctgtt gcccttgccg tcggaattgt tcttgtacag gacatactgt tcgactcccc
     1921 tcacccgagc catttcctga agacacgtcg cgagaatgag ctctccagct cgttcgaacg
     1981 ccactacctc tcgggcgttc gagcactccg gcgtcgagta ctccggatga gctccatcga
     2041 catataaacg tccgccgttc gccagcacct tgttcagctg tcggttatag tctggattgg
     2101 gacgttctcg ctcgccgtcg acttcaaatc cccgcgcatc taagagcgga ttttcattct
     2161 cgtaatccca gagagcctcc ggagccggaa gtcctgggta atgtccgatc acagcgaatg
     2221 agccggacac agggtccatt gcggatggat cttttgccgc gatgccgaac tcggtttcag
     2281 tgccaagcac gcgcatgcga ttcgtggatg tgtggtctgg cataggatag ctagagatag
     2341 tggccggtac tgatggtttc aatctgccga tgttcggtgg gcccaccact gatggtccgg
     2401 atatgaataa ttttctcgcc cttctttccg gcgactttcg cccaatcgtc cggattggtc
     2461 gtattgggga gatcttcgtg ttccttgaac tcctcgcgaa tggcgcggat caagtcgtcc
     2521 gagcgcaagc cagtcccctc ttgggc
//
LOCUS       DASTWE010000485         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1241271, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000485 DASTWE010000000
VERSION     DASTWE010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241271"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..805
                     /locus_tag="VFT30_05110"
     CDS             68..805
                     /locus_tag="VFT30_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU5092042.1"
                     /translation="MTAKLEPELLPKHVAVIMDGNGRWAELRGLPRIAGHREGINSVR
                     EMITLCLELGIHALTIYAFSQENWNRPTQEISALMGLLEHYLSTERASLIEQGVCFRA
                     IGRHELLPPSAQHWVHTTEKETAHLDKLILTVALSYGGRAEIVDAVKTLVEDVQTGTL
                     QPTLIDEPTIQQYLYTHPLQDPDLLIRTSGETRISNFLLWQLAYTELCFTPTLWPDFR
                     RREFLLALIEYQRRERRFGRVLSTVSS"
BASE COUNT          236 a          311 c          256 g          220 t
ORIGIN      
        1 gaacgcgctc taggctcgta tgacacatca ccaggcttca agtttcgatc acttatccga
       61 gagcgaattg accgccaaat tggagccgga actgctcccg aaacatgtcg cggtcatcat
      121 ggacggcaac ggccggtggg cggaactccg tggtctccct cgtattgcag ggcatcgcga
      181 aggcatcaat tccgtcagag agatgattac cctttgcctg gaactcggca tccatgccct
      241 gaccatctat gcgttttcgc aagaaaattg gaaccgtccg acgcaagaaa tcagcgccct
      301 catggggctg ttggagcatt acctttctac ggaacgggcc agtttgattg aacaaggtgt
      361 ttgcttccgc gctatcggcc gccacgaact gcttccccca tcggcccaac actgggtaca
      421 cacgaccgaa aaagaaacgg cgcacctcga taagctgatc ctgacagtcg ccctcagcta
      481 tggaggacga gcagaaatcg tcgacgcagt gaaaactctg gtcgaggacg ttcagacggg
      541 aacgctacaa ccgaccctta tcgatgaacc cacgatccag caatacctct acacacaccc
      601 actccaggac ccggacttac tgattcggac gagcggagaa accaggatca gcaactttct
      661 cctgtggcaa ctggcctaca ctgagttgtg cttcacgccc actttatggc cggattttcg
      721 gcgacgggag tttctgctgg cgctgatcga atatcaaaga cgggagcgcc gctttggcag
      781 agtgctgagc accgtgtctt cgtaaccctt gtgggacatc cacccagcac cgaagcgatc
      841 acccgctcga tatccgtgac caccccacca gcacgtacac gacacttcga tctccggcgt
      901 ctctatacgg cagctgcgct tatcccggcg gtctatgtca tcatcgtaca tctcgctcca
      961 tgggccttga cactgctgtt gatcgcggta ggctccattg cttttatgga gttctatcaa
     1021 atc
//
LOCUS       DASTWE010000486         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_950367, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000486 DASTWE010000000
VERSION     DASTWE010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950367"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1128)
                     /locus_tag="VFT30_05115"
     CDS             complement(226..1128)
                     /locus_tag="VFT30_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5092043.1"
                     /translation="MVLNPIDAQAVTFQQISVNQLSFRVATAGSGDRLVLCLHGFPES
                     AVSWRYQIAPLAQAGFLVWAPDLRGYGGTTRPTGIDAYRIESLMDDVTGLLDAAQVQR
                     AILVGHDWGGIIAWYYAMRHADRVEALVIVNAPHPACFEREVRHWRQLRRSWYMGFFQ
                     IPWLPEAALSAGHGYVIGEIFGRMTIHREQMPEDIVRLYRRQACEPGALAAMVNYYRA
                     ALRGGGAMRQRRMGYPIVPVPTLVIWGLQDHALVHQNLDGLNDFVTDLTVVKIADAGH
                     FVHEDKPEQVTHEMLTWLQNHVSL"
BASE COUNT          265 a          337 c          308 g          232 t
ORIGIN      
        1 tcttactccg ctcctaggca atcccttccc tgcagggacc aacccttcgg caatcgccac
       61 gcccgcccag acatcgtgag gaccgtttac acggttacgt acgcgcagta cgtggtccgc
      121 tcatgatggg ggatgagaaa cgtagtaata tggtctcgcg cagtggagaa catcagaagt
      181 cgttggcggg ctcgtaaggg aaggaaggta gcatgggtta acgaattaga ggctcacgtg
      241 attctgcaac caggtcagca tttcatgcgt gacctgttcc ggcttgtctt catgcacgaa
      301 atgtccggcg tcggcgatct tcaccaccgt cagatcggtg acgaaatcgt tcagcccatc
      361 aagattctga tggacgagtg catgatcttg taatccccaa attaccaatg tcgggacagg
      421 aacaatagga tatcccatcc ttcgctgacg cattgccccg ccgcctcgta aggccgctcg
      481 ataataattc accatggccg cgagtgctcc cggctcgcaa gcctgccgtc gatacagccg
      541 aacgatatcc tcaggcatct gctcgcgatg aattgtcatg cgcccaaaga tctcaccgat
      601 tacatagcca tggccggccg acaatgccgc ttccggaagc cagggaattt ggaaaaagcc
      661 catgtaccag gaacggcgca actgacgcca gtgccgtact tcccgctcaa agcatgccgg
      721 gtgaggcgcg ttcacaatca ccaaggcttc cacgcgatcg gcgtgccgca ttgcatagta
      781 ccaggcgatg atgccgcccc aatcatgccc gaccagaatg gcgcgttgaa cctgagcggc
      841 atcgagcagt ccggtgacat catccatcaa ggattcaatt cggtaggcgt caatgcctgt
      901 tggccgtgtc gtcccaccat atccccggag gtccggggcc cagaccagaa atccggcctg
      961 cgccagcgga gcgatctggt accgccatga gacggcggat tctggaaacc catgtaggca
     1021 gagcaccaat cgatcgccgg acccggctgt tgccactcga aaggacagtt ggttcacgga
     1081 tatctgttga aacgtcactg cttgtgcgtc aataggattg agaaccatgc gcttatccta
     1141 ga
//
LOCUS       DASTWE010000487         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1596961, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000487 DASTWE010000000
VERSION     DASTWE010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596961"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..>1099)
                     /gene="proB"
                     /locus_tag="VFT30_05120"
     CDS             complement(260..>1099)
                     /gene="proB"
                     /locus_tag="VFT30_05120"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU5092044.1"
                     /translation="AFERLNIQVAQILLTHQDLADRRRFLNARHTLAALIGFGIVPII
                     NENDTVAVDEIRVGDNDTLASEVAHLADADLLVILSDVDGLYTEDPRKNPSATLIPLI
                     TEITPDIERLAGVSSTFEGTGGMATKLRAAKKVGEYGISTLVLNGEQAGLLPTVLGGG
                     PGGSLFLARERRLNSRKHWIAFTLRSRGQIHLDQGAVDALSNRGKSLLASGVLRVTGS
                     FEAGDPVSCVDTDGKEFAKGLVNVPSDLLGQMKGLKTADIHDQFGPQEYEEVIHRDNL
                     VIL"
BASE COUNT          249 a          286 c          295 g          269 t
ORIGIN      
        1 attcgctgga gccagtgacc cgccccgttt atggggcgga tcgccggtcg ggatgaatag
       61 gacctgagag agcttcatgc gctcatatac gtgatgagca atggtcagat gacaattatg
      121 aataggattg aagcttccac ccaataagcc gaggcgcatg aagcaatgtt gaatgtttaa
      181 tggttaatgt tcaaggtatg agtgtggttc agcggatctt gatgtcattc attcaacatc
      241 aaccattgat catgtcttgt tacaggatca ctaagttgtc tcggtggatg acttcctcat
      301 attcttgggg cccgaactga tcatgaatgt ccgctgtctt gaggcctttc atctggccca
      361 ataagtcgga cggaacgttc accaagcctt ttgcaaattc ctttccgtct gtatcgacac
      421 aactgactgg atcaccggct tcaaatgatc ctgtcacccg gaggacgccc gatgccagca
      481 ggcttttccc tcgattgctc aaggcgtcca ccgccccctg gtcgagatgg atctgaccgc
      541 gggagcgaag cgtaaaggcg atccaatgtt tacggctgtt cagccgacgc tcccgggcaa
      601 gaaagaggct tccacctggc ccgccgccaa ggaccgttgg gaggagtcct gcctgctcgc
      661 cattgaggac taaagtcgaa attccatact cgccgacttt cttggctgct cgaagcttgg
      721 tcgccattcc gcctgtacct tcaaatgtgc tggaaactcc tgccaggcgc tcgatatctg
      781 gggtaatctc cgtaatcagt ggaatcaagg tcgccgatgg gtttttacgc gggtcttccg
      841 tatatagccc gtcgacatca gagagaatca ccagcaagtc cgcatcggcc aggtgagcca
      901 cttcgctcgc gagggtgtcg ttgtcgccga ccctgatttc atcaaccgcg acggtatcat
      961 tttcattgat aatgggcact atgccgaaac cgatgagtgc cgcgagtgta tggcgagcgt
     1021 taagaaatcg acggcgatcg gcgagatcct ggtgggtaag cagaatctgc gccacttgaa
     1081 tattgagacg ttcaaatgc
//
LOCUS       DASTWE010000488         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1241317, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000488 DASTWE010000000
VERSION     DASTWE010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241317"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1015)
                     /locus_tag="VFT30_05125"
     CDS             complement(<1..>1015)
                     /locus_tag="VFT30_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092045.1"
                     /translation="RSDSPEERVIRLAKILPPKAPILTGKALERVVKQLGKQEPQNPI
                     EEVRQQNQELLRALEELHERQQELVRLNGELEDTNRGVVALYAELDEKAGHLRRADEM
                     KSRFLSNMSHEFRTPVNAILALSHLLLEHTDGELNGEQTKQVNYIRKSGEDLLELVND
                     LLDLAKIEAGKVTVKASKFEVANLFSALRGMLRPLLASNAVSLVFEESEGLPSMTSDE
                     GKVSQILRNFISNAIKFTERGEVRVAAALTPAGDSMVFSVADTGIGIAPEDQERIFEE
                     FSQLDNPIQKQVKGTGLGLPLCKKLAQLLGGAIEVRSRVGEGSTFVATIPVNYAVSDI
                     DRS"
BASE COUNT          206 a          308 c          264 g          237 t
ORIGIN      
        1 tccgatcgat cgatatcgct caccgcgtaa ttgacgggaa tggtggcgac aaaggtcgac
       61 ccctcgccca ctcggctcct gacctcgatc gcaccgccga gcaactgagc caacttcttg
      121 cacagcggaa gccctaaccc cgtgcccttc acctgctttt gaatgggatt gtccagctga
      181 gaaaattctt caaaaatgcg ttcttgatct tccggcgcga taccgatgcc cgtgtcggcc
      241 acggaaaaga ccatggaatc accggcaggc gtcaaggctg ctgcgacacg cacctcaccg
      301 cgctcggtga atttgatcgc attcgaaatg aagttccgga gaatctgaga cactttgcct
      361 tcgtccgacg tcatggatgg aagcccttcc gattcctcga agacgagact caccgcattt
      421 gacgcgagca gcggtcgaag catcccgcgc agggcactga agagattggc aacttcgaat
      481 ttggatgcct tcactgtgac tttgcccgcc tcgatcttcg ccaaatcgag aaggtcgttg
      541 accaattcta ggaggtcttc gccgctcttg cggatataat tgacctgttt ggtctgctcg
      601 ccgttgagct ccccgtcggt gtgttcgagc agcaggtgcg acaaagccag aatcgcgttc
      661 accggtgtcc ggaattcatg actcatattg gaaaggaagc gtgatttcat ttcgtccgcg
      721 cggcgcaagt gccccgcctt ttcatccaac tcggcataga gggcgaccac accacgattg
      781 gtgtcttcca gttcgccgtt gaggcggacc agttcctgtt gtcgctcgtg caattcctcc
      841 aaggccctga gcaattcctg attttgctgc cgcacctctt caatggggtt ctgtggctcc
      901 tgcttcccca actgcttgac gactctctcc aatgccttgc cagtcaaaat cggcgccttc
      961 ggtggaagaa tcttcgccag gcggatgacg cgctcctccg gcgaatcgga cctga
//
LOCUS       DASTWE010000489         1314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_45764, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000489 DASTWE010000000
VERSION     DASTWE010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45764"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFT30_05130"
     CDS             <1..331
                     /locus_tag="VFT30_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEU5092046.1"
                     /translation="FQLNLIALLAGAFLNNPWTLIPVLGGTYWTGATLLGLREFPSFD
                     WSDLTFMGIYHQVMPYVWPFFVGGMVLSFAGALVSYPLVYLLLSKYRQGRAIKHPEPL
                     PPPPSLR"
     gene            358..1245
                     /locus_tag="VFT30_05135"
     CDS             358..1245
                     /locus_tag="VFT30_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5092047.1"
                     /translation="MTPPAAQPPAPHDGSALIADPIHEYLSFTVPYATPDGLERTEKD
                     LIDSSWVQRLRYIYQLQSARWVYPSAEHTRFVHSLGTMHVAGRFARHLYPFLQKAVPD
                     VPSSAFVEEFLRITALVHDIGHGPFCHFFDDNYLHGFGLSHEKLGQIIVREHLGPIIK
                     RIRRSPSGPFARGEALNPDLIAHVILKEKGKDNSRVPRWINFLQPVISGSYTADNLDY
                     VLRDAYMCGVAVGPVDLTRLIHYTIVTDKGFTIHKTGLPALHMFLNTRMYLYSNVYYH
                     RTTRAIDIHLRDIFGETMK"
BASE COUNT          261 a          403 c          331 g          319 t
ORIGIN      
        1 attccaattg aatctgatcg cgctgctcgc cggcgccttt ctgaataacc cctggacgct
       61 gatccccgtc cttgggggga cttattggac gggcgcgacg ttgttgggcc tgcgtgaatt
      121 tccgtccttc gactggagcg acctgacctt catggggatc tatcaccagg tcatgcccta
      181 tgtctggccg ttcttcgtcg ggggaatggt tctgagcttc gccggtgctc tcgtgtccta
      241 tccgctcgtg tacctcttgc tttccaaata tcgccagggc agggcgatca aacaccctga
      301 accgttgccc cctccaccaa gcctgcgcta agatcggcgc atccccccat tggacgcatg
      361 acacctccag ccgcacaacc ccccgctccc catgatggat cggccctcat cgcggatcca
      421 atccatgaat acctgtcctt caccgttcct tacgcaactc ccgatggatt ggagagaact
      481 gaaaaggacc tcatcgactc ttcctgggtc cagcgactgc gttacatcta ccaattgcaa
      541 agtgcccgat gggtatatcc gtcggccgaa catacgcgct tcgtgcactc gctggggact
      601 atgcatgtgg ctggacgctt cgcgcgccac ctgtacccct tcttgcagaa agcggtacca
      661 gacgttccgt cgtctgcctt cgttgaagag tttctgcgga tcaccgcgct cgtacatgat
      721 atcggtcatg ggcctttctg ccatttcttt gatgacaact acttacacgg tttcggactg
      781 tcgcatgaaa agctcggcca gattattgtg cgcgagcatc ttggacccat catcaaaagg
      841 attcgccgta gtccttccgg tccattcgct cgcggtgagg cgctgaatcc ggatctcatc
      901 gcgcacgtca ttctcaagga gaagggcaag gacaactcgc gggttcctcg ctggatcaac
      961 ttccttcagc cggtcatttc aggaagctat acagctgaca atctggacta tgtcctgcgt
     1021 gatgcgtaca tgtgcggtgt ggcggtagga ccggtcgatt tgacccgctt gattcattac
     1081 accatcgtca ccgacaaggg cttcaccatt cacaagaccg gcttgccggc cctgcacatg
     1141 ttcctcaata cgagaatgta cctctactcg aacgtgtatt atcaccggac cacccgcgcg
     1201 atcgatatcc atttacggga catcttcggg gagaccatga agtgactgtt tccgtggaac
     1261 ccggccacac acatggacag gtatctcacg ttgacggact ggtcattgct ggaa
//
LOCUS       DASTWE010000490         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1435415, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000490 DASTWE010000000
VERSION     DASTWE010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435415"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="VFT30_05140"
     CDS             <1..2017
                     /locus_tag="VFT30_05140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEU5092048.1"
                     /translation="GFSLFDANDRLVICNRKYREMVSQPGAEVQPGDSFESIICRIAK
                     RGDIPAAIGDIDSWVKMRLDMHRNPQGPYIQQRSSGEWVMINERKTQDGGTVALTTDI
                     SALKNAEQAAEDAKARLADSLALVEAAKARMQEELNVGRDIQRSMLPRVFPAFPDRKE
                     LELYAVLEPALEVGGDLYDFFLLDDHRLCFVIGDVSGNGVPAALFMAMTKIMVKTREA
                     SDPSPASIVTHVNDALSAENDSCMFVTLYLGILNLRDGTLLTTNAGHNPPLLKRRNGK
                     FEWLTAQDGPLVGPMPGIAFKESLIQLGPGDEIFLYTDGVTEADNVRRELFGKDRLKT
                     VLDQSRAVSVIDRIGEVMNAVRGFAGSAPQADDITILGIRYLGIAPSDVAVSVFRQTM
                     LNRLAVIPMIQIAFERYVAQWERAKPLIPTLNMALDDLLNNVVQYAFPNDPTEHTIEV
                     EGEVRDEYVALTITDDGIPFNPLTVATPDLSLLLHEREIGGLGIHLVRSMFDEVTYHR
                     NVGRNVLTVKKKFVPEPSASMRRPDRTGIGAFEDEWKPPQPDQHVRSMTMSVTSHQRG
                     DVMIVTPQNRFDTNSAPEVERILMEHIERGERKILLDLSQISYISSIGLRVILKAVMT
                     MSRAGGRVVLSGGNDHVRTVLQLSGAMVMSLHASTLEDAISKVRETR"
     gene            2208..>2510
                     /locus_tag="VFT30_05145"
     CDS             2208..>2510
                     /locus_tag="VFT30_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5092049.1"
                     /translation="MDQETRHTLAVTSRENAGITFVKMQGSLSATTAEQGLQEMKKIV
                     DSGAKKIVVNLADVDYISSGGIRVLILSSKQLNNVHGEMKIAAAKGMVKEALEAGGF"
BASE COUNT          599 a          659 c          740 g          512 t
ORIGIN      
        1 aggattttcg ctcttcgacg caaacgatcg cctggtgatc tgtaaccgca agtatcggga
       61 aatggtgtct cagcccggcg ccgaggttca accaggcgac tcgtttgaat cgatcatctg
      121 ccgcattgcg aaacgaggcg atatccccgc agcgatcggg gatatcgatt cctgggtgaa
      181 aatgcgactc gacatgcacc gcaatcccca agggccatac attcaacaac gttccagcgg
      241 agagtgggtc atgatcaatg agaggaagac ccaagacggc ggcacagtcg cgttgaccac
      301 cgacatttcg gccctgaaaa atgccgaaca agccgccgaa gacgccaagg ccaggctcgc
      361 ggattcgttg gcgctggtcg aagcggcgaa ggcccgcatg caggaagaac tgaatgtcgg
      421 acgggatatt cagagaagca tgttgccgcg cgtgtttccg gcctttccgg atcgaaagga
      481 gctggaactc tacgccgtcc tcgaaccggc tctggaggtc ggtggcgacc tctatgattt
      541 ctttctgctc gacgaccatc ggctgtgttt tgtgatcggc gatgtgtcgg gcaacggggt
      601 tcctgccgcc ttgttcatgg caatgaccaa aatcatggtg aagaccaggg aggcgtccga
      661 tccctcaccc gccagtatcg tcacacatgt gaacgatgcg ctcagcgcgg agaacgatag
      721 ttgtatgttc gttaccctct acctgggcat cttgaatctt cgcgacggca cgctcctcac
      781 gaccaatgcg ggccataatc ctccgttgct gaagcgacgg aatgggaagt ttgagtggtt
      841 gacggcccaa gatgggcctc tcgtcggtcc catgccgggc atcgccttca aggagagcct
      901 catccaatta ggaccggggg atgagatctt cttgtacacc gatggggtca ccgaagccga
      961 taatgtccgg cgcgaactgt tcggtaagga ccggttaaaa acggttcttg atcaatccag
     1021 ggctgtctcg gtgatcgatc gcatcggtga ggtcatgaac gcggtaaggg ggtttgccgg
     1081 tagcgccccg caggcggacg atatcaccat attggggata cggtatctcg gcatcgcccc
     1141 gtccgatgtg gcggtgagcg tatttcgtca gacaatgctc aaccgattgg cggtcattcc
     1201 catgatacag atagcgtttg agcgatatgt ggcgcaatgg gaacgggcca agccgctgat
     1261 ccccacactc aacatggcgc tggatgatct gctcaacaac gtcgtgcagt atgccttccc
     1321 gaatgatcca acggagcaca ccattgaagt ggaaggcgaa gtgcgcgatg agtacgtggc
     1381 cctgaccatt acggatgatg ggattccctt caatccctta acggtcgcca ccccggatct
     1441 gtcgctgctc ctgcatgaac gcgaaatcgg aggtttggga attcacttgg tacgctctat
     1501 gtttgacgag gtgacgtacc atcgcaacgt gggacgcaac gtcttgacgg tgaaaaagaa
     1561 gttcgtacct gagccgtccg cctccatgag acgtcccgat cggactggaa tcggggcgtt
     1621 cgaggacgaa tggaagccgc cacaaccaga ccaacacgtg aggagcatga ccatgagtgt
     1681 cacatcacat caacgtggtg acgtgatgat cgtcaccccg cagaaccgct tcgacacgaa
     1741 tagcgcaccg gaagtcgagc gcatcctgat ggagcatatc gagcgtgggg aacgcaaaat
     1801 tctactggat ctctcacaaa tttcgtacat ttcatccatt ggattgcggg tgatcttgaa
     1861 agccgtgatg accatgtccc gagcaggcgg aagagtcgta ctcagtggag ggaacgacca
     1921 cgtcagaacg gtgctccaat tgagtggggc gatggttatg agcctccatg cctccacgct
     1981 tgaagacgcc atctcgaagg tccgagagac acgctaatgc cgcctttcga ttggtcgaaa
     2041 cgcagtaaat cgttgacgct ccgtctttga tatcgttcgg atggaagccg ggaacctatc
     2101 gatggtatga tggggcgcgc tggagcaata gtgggctcgg ttcataaggc cactgcgcga
     2161 acgagattaa ttactgggga agaagcacat aaaaaaggag tgcacccatg gaccaagaga
     2221 cccgacacac gttggcggta acttctcgtg agaacgccgg catcaccttc gtcaagatgc
     2281 aggggagcct ttccgccacc acggcggaac aagggctcca ggagatgaag aaaatcgtag
     2341 actcgggagc gaaaaagatc gtcgtcaacc tggctgatgt ggattacatc agcagtggcg
     2401 gcatccgggt tctcatcctg tcgagcaagc aactcaacaa cgtccatgga gagatgaaga
     2461 ttgccgcagc gaaggggatg gtgaaagaag cacttgaagc cggtggattt
//
LOCUS       DASTWE010000491         1468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_692172, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000491 DASTWE010000000
VERSION     DASTWE010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692172"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFT30_05150"
     CDS             complement(<1..884)
                     /locus_tag="VFT30_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092050.1"
                     /translation="MPIVAMLFLVPFFTWLGVRQAVANDRLIEELETDPKLAKTHHRK
                     VEPWRPGWARELHVWPYLVRIEFLATVIVTVVLFIWSITLNAPLEEPANPNLTMNPSK
                     APWYFLGLQEMLVYFDPWIAGVVMPSIIMVGLMVFPYVDSNPLGNGYYTLRQRRFALW
                     MFGAGFLMWIVLIVIGTFIRGPGWIWFWPGQTWDHNAVVFDKNVDLHDMIAQSSIGKA
                     LHLGFLATNPGKFIFGLIVVSGFYLVGALFFHWLMTVDFKKMRMPRGLSLTELHSALF
                     PKNEFEQKLLARTSLLQYL"
     gene            complement(1125..>1468)
                     /locus_tag="VFT30_05155"
     CDS             complement(1125..>1468)
                     /locus_tag="VFT30_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU5092051.1"
                     /translation="FTGYLLPDDQLGFWAVTVGTNMARATPMLGHEGPFGPQLGLTAY
                     NDVRFGLLGGSIVDSNALLRSYIWHCIGIPLVASIFMAVHFWRIRKDGGISGPAPVML
                     ESEIKAVAKKK"
BASE COUNT          375 a          402 c          383 g          308 t
ORIGIN      
        1 agatattgca gcaggctagt gcgcgccaga agcttctgtt cgaattcgtt cttgggaaac
       61 agggcgctat gaagctcagt taaactcaat ccacgcggca tcctcatctt cttgaagtca
      121 accgtcatca gccagtggaa gaagagcgcg ccgacaagat agaagccgct gaccacgatc
      181 agtccgaaga taaacttgcc gggatttgtg gccagaaaac cgagatgaag agctttgccg
      241 atagagctct gcgcgatcat gtcgtgcagg tcgacgtttt tatcgaatac aaccgcgttg
      301 tgatcccacg tttgtcccgg ccagaaccag atccagcccg gtccacgaat aaacgtgccg
      361 atcacgatta ggacgatcca catcaaaaag cccgcgccaa acatccagag tgcgaagcga
      421 cgctgtcgca gcgtgtaata gccgttgccg agtggattgg agtcgacgta cggaaagacc
      481 atcaacccga ccatgatgat cgacggcatc acgacgcccg cgatccacgg atcaaagtag
      541 accagcattt cctgtagccc gaggaagtac cacggcgctt tcgacggatt catcgtcagg
      601 tttgggttgg ctggctcttc gagcggagcg ttgagcgtga tggaccaaat aaaaagaacc
      661 accgtgacga tcacggtggc cagaaattca attcgaacaa ggtagggcca aacgtgaagc
      721 tctcgtgccc aacccggtct ccatggctcg actttgcgat gatgcgtctt cgccagtttg
      781 ggatctgttt cgagctcttc gatgagccgg tcgttagcga cagcctgcct gacgcccagc
      841 cacgtgaaga agggcacgag gaagagcatc gcaacgatcg gcacgttatc gggcgccgag
      901 gagatgatcc acaactggtg ccaatcctgg acgttaatcg cccagatgac tgtgatcagt
      961 aagagcgctc cgagaatggt ccacttgttg atgaatcttc gcataagtgc ttctatcctg
     1021 aaatcatgtg aatcttgtca tcctgtctaa aaactaccgt gctgccttgg ctggaaaacc
     1081 agttttacag gattacaaga tttacaggat taacatgatt tcaatcattt cttcttggcg
     1141 actgccttaa tctctgactc gagcattacc ggcgccggac cggagatgcc tccgtccttc
     1201 ctgatacgcc agaagtggac tgccataaat atcgacgcaa ccagcggaat gccgatgcag
     1261 tgccagatgt acgaacgcaa caacgcattc gagtcgacga tcgatccgcc gagcaatccg
     1321 aagcgcacgt cattataagc agtcagtccg agctgtggtc cgaacgggcc ttcgtgtcca
     1381 agcatcggcg tcgcgcgcgc catgtttgtg cccactgtca ccgcccagaa accgagctga
     1441 tcatcaggca acaaataacc cgtaaacg
//
LOCUS       DASTWE010000492         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_983111, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000492 DASTWE010000000
VERSION     DASTWE010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983111"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFT30_05160"
     CDS             <1..186
                     /locus_tag="VFT30_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092052.1"
                     /translation="FSLFALVELHLPFPALFLAWSPVGSDVLIVHLTTALVAAYYGYY
                     WTDAVVEAGHRQPIRTA"
     gene            686..1360
                     /locus_tag="VFT30_05165"
     CDS             686..1360
                     /locus_tag="VFT30_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5092053.1"
                     /translation="MDVGASGIKAVLLNELGDLVGRRIRRDTPSSGMPGEVMDTIVAS
                     AKDLESFDRVAVGFPGIIVDGIVQQAPNLPPEWKDFNIAEVLQRRLKKPVRVANDADV
                     QGFGAIAGKGVELVVTLGTGVGTALFTDGHLVPNAEFGKAKLRNEMLQKEGKKKWNRR
                     LSKFIRRLEEKFHFTRLYIGGGNSRNVDISLLPSNVTIVSNLNGLVGGIALWRDRETA
                     REDTNG"
BASE COUNT          376 a          367 c          427 g          336 t
ORIGIN      
        1 tttagtcttt ttgcgctcgt cgaattacac ctgccgtttc cggcattgtt tctagcctgg
       61 tcgccggtcg gtagtgatgt cctcattgtg cacctgacga cggctttggt tgctgcctat
      121 tacggctatt actggacgga cgcggtggtg gaggcgggcc accggcagcc cataagaacg
      181 gcatgacatc gaacagtcga gatcttcaag gacgaagctt acattacaag cctggcaaga
      241 accgggccgg gtgggcaagc gcgaatccgt caagcaaagg aacgtgagaa gctttgtccg
      301 gaacggggag actatacgac gcggatgatc ttcatattcg agaagccctc ttgcagcaat
      361 tggaatccag ctcagttgat gcatcaagat tgaagacctt gttgcgtgaa gttctgattc
      421 accagcgatg gaggaagata aaaagttaaa ctgaacgttc ggcgaaagta attttgtatt
      481 tgtgtctttg gctcttaatt cggcagcgga aaattgtctc gcgcggacag gcgcacaacc
      541 tatgcagtct tcgagtttat caaatagaga acagaagcgg cgaaaacata ccgttcggcc
      601 cagtcacaga ccgtcggtag gccaggttcc gcacagtcag gctcgcggga cctccgctaa
      661 gtcccgctcc ccattgacat tggtcgtgga cgtgggcgcc agtggaatca aggccgtcct
      721 gctcaacgaa ttgggagatc tcgtcgggcg gcggatccgc cgtgacaccc cgtcttccgg
      781 catgcccggc gaagtgatgg acacaatcgt cgcatcggcc aaggatttag aatcattcga
      841 cagagtggcg gtcggatttc caggcatcat tgtagatggc atcgtgcaac aagctccgaa
      901 ccttcctccc gaatggaaag atttcaacat cgccgaggtg ttgcaaaggc gattgaagaa
      961 accagtccga gtggcaaatg atgccgatgt gcaaggtttc ggagcgattg ccggaaaagg
     1021 agtcgagttg gtcgtgacat taggtaccgg cgtcggcacg gcactcttca ctgacggaca
     1081 tctcgttccg aacgccgagt tcggcaaagc caagttacgg aatgaaatgt tgcagaaaga
     1141 ggggaagaag aagtggaacc ggcgcctgtc caaattcatt cgccgactcg aggaaaaatt
     1201 tcattttacg cgcctataca tcggtggagg caattcgagg aatgtggaca tctcactgct
     1261 tccctctaat gtcacgattg tttccaactt gaacggattg gtcggcggaa tcgcgctgtg
     1321 gcgtgatagg gagaccgccc gtgaggatac aaacggctga tcgtggacca gtctcggagt
     1381 ctaaggataa cgacggtcga ttggctctca cgggccgtag ctaaaaagag atggagaata
     1441 ggaaatactc cgaacttttt caaagcaccc gtccgataaa gactcagaat ccttcattca
     1501 tatgct
//
LOCUS       DASTWE010000493         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_433638, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000493 DASTWE010000000
VERSION     DASTWE010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433638"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VFT30_05170"
     CDS             complement(<1..1340)
                     /locus_tag="VFT30_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5092054.1"
                     /translation="MNLGFFIDRPIFASVLSIVVVVVGLVAMLALPIAQFPEITPPVV
                     QIEADYPGASAEVVAESVARPIELQLPGIDRLLYFDSTSTNDGHMTIKLTFEIGTNVD
                     IAQVQTQNRQKLAEPQLPPEVVRQGVTVKKMSPDLLTVMVLNSDDPQQDALFLSNFAI
                     LRILDNIKRLPGVGDALVFGQQNYSMRLVLDPVRMAQLDLTPTDITTVVREQNRDFPA
                     GTVGREPMPIPTELTLPLITEGRMTDVREFEDMIVRALPNGSMVRLKDVARVELGAQS
                     YVLEGRFNRKPTALLLTCLSPGANALDTVKRIRGEMDRLSKIFPPGVTYDIPYDTTRF
                     VEVSIKEVVKTLGEAMVLVVLVVYLFLQSLRATLIPVLAVPVSLIGTFAGMAALGFSI
                     NTLTLFGLVLAIGIVVDDAIVVVENVERHMAAGLSPKDAAKKAMGEVAGPVIAIV"
     gene            complement(1337..>2521)
                     /locus_tag="VFT30_05175"
     CDS             complement(1337..>2521)
                     /locus_tag="VFT30_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5092055.1"
                     /translation="NETTRRRLSLSSVLLMGVFGLLGCKGEAGSPVPRPIPEVQIVET
                     TSQTVPDEPEFIGQAEASSIVEIRPQVTGIIKRRFFSEGRDVKQGDRLYEIDPVPFQA
                     AFASAKGRVAQAEARVVQAKQDLARVKPLLVEDAVSQKDVDDAIAEDLAARAALEAAR
                     GDLVKAKFDLDNTLIVAPVDGLIERTRYYQGRLVTAQTDLLTVIHQVDPMYVIVSAPE
                     SFLLKRRRDTLSKRIQHPGIYKLTGTIIFVDGTTYAHDGVLDFADIGLRVETGSRQAR
                     VVFPNPDRILLPGQFVTVRFHGVSKPNAILVPQRAVQQGPKGAVVYVVSQGDQVEVRD
                     VKATDWRGDQWLIEDGLRPGERVIVEGFQRIVPGAQVKPVTVVSTRSDTSSSAAGVEQ
                     SK"
BASE COUNT          548 a          742 c          700 g          531 t
ORIGIN      
        1 acaatggcga tcactgggcc ggccacctcc cccatggctt tcttggccgc gtccttcggg
       61 gacagccctg ccgccatgtg acgctcgacg ttttccacga ccacaatggc atcatccacc
      121 acaatcccga tggcgagtac caagccgaag agagtcaggg tattgataga gaacccgaga
      181 gctgccatcc ccgcaaaggt tccgatcagc gagaccggaa ccgccaaaac aggaatcagt
      241 gtggcacgca agctttgaag aaagaggtat accacgagga cgaccaagac catggcttct
      301 cccaaggtct tcaccacttc cttgatggag acctcgacaa aacgcgtcgt gtcgtacgga
      361 atgtcgtacg tgacgccagg cggaaagatt ttgctcagtc tgtccatttc tccgcgaatt
      421 cgcttgaccg tgtcgagagc gttggcgccg ggggagagac aggtgagaag cagcgcggta
      481 ggcttgcggt taaagcgccc ttcgagcacg taagattgcg cccccaattc gacccgcgcg
      541 acatctttaa gccgaaccat cgatccgttc ggcagagctc gcacgatcat atcctcgaat
      601 tctcgcacgt cggtcatgcg cccctccgtg atgagaggaa gcgtgagctc cgtgggaatc
      661 ggcatcggct cccggccgac ggttccggca ggaaaatcac ggttctgttc gcgcacgaca
      721 gtcgtgatat cggtcggcgt cagatccagt tgcgccatac gcaccggatc gaggacgagg
      781 cgcatgctgt agttttgttg tccgaagacc agtgcatcgc cgactccggg cagccgcttg
      841 atattgtcga ggattcgcag gatcgcaaaa tttgagagaa agagcgcatc ttgctgcgga
      901 tcgtctgaat tcaacaccat aaccgtcagc agatccggcg acattttttt aacggtcact
      961 ccttgacgaa cgacttccgg cggaagttgt ggctcggcta gcttctggcg attctgcgtc
     1021 tggacttgcg cgatgtcgac gttcgtaccg atttcgaagg tcagtttgat agtcatatgc
     1081 ccgtcgttcg tgctcgtcga atcgaagtag aggagccggt cgatgccggg caactgcaac
     1141 tcgatgggac gcgcgactga ttccgccacg acctccgcgc tcgcaccagg gtagtcagct
     1201 tcgatctgca ccaccggagg agtgatttct ggaaattgag cgatgggcaa ggcaagcatg
     1261 gccaccaatc ccaccaccac aacgacgatg gaaaggactg aggcaaaaat cggccggtca
     1321 atgaaaaacc cgagattcat tttgactgtt ccacgccggc cgctgatgaa gacgtatcgg
     1381 atcttgttga gaccacagtc accggtttga cctgggctcc aggcacgatt cgctggaatc
     1441 cctcaactat gacccgctcc ccgggacgga gcccgtcctc gataagccac tgatctcctc
     1501 gccagtccgt cgctttcaca tctcgaactt ccacttgatc gccttgactg acgacataga
     1561 cgaccgcgcc ttttggccct tgctgaaccg cgcgttgtgg aacaaggatg gcattgggct
     1621 tcgatactcc atgaaagcgc acggtcacga actggccggg cagcaatatc ctatcggggt
     1681 ttgggaatac cacccgcgct tgccgggagc cagtctcgac ccgcaatcct atatccgcaa
     1741 aatccaacac gccatcatgc gcataggtcg ttccgtccac gaagatgata gtacccgtca
     1801 acttgtagat ccctggatgc tgaatccgtt tcgaaagagt gtcccgtcgt cgtttgagca
     1861 agaaactctc cggcgcgctc acgatcacgt acatgggatc gacttgatga atgacggtca
     1921 gcagatccgt ttgtgcggta accagccgac cctggtagta acgtgtacgc tcgatcaagc
     1981 cgtccaccgg ggctacgatg agcgtgttgt ccaaatcgaa cttggctttg accagatctc
     2041 ctcttgcggc ctctagcgcg gcccgcgcgg caagatcttc cgcgatcgcg tcgtcgacat
     2101 ctttctgact gacggcatcc tcaacgagta acggcttgac acgagccagg tcttgcttgg
     2161 cctggacgac acgcgcctca gcttgcgcga ctcgtccctt cgcgctcgcg aatgccgctt
     2221 ggaagggtac gggatcgatc tcgtaaagac gatcgccttg cttgacgtcc cgtccctcgg
     2281 aaaagaagcg tcgcttgatg attccggtga cttgcggacg gatttcgacg atgctcgacg
     2341 cttcggcttg cccgataaac tccggttcat cagggacagt ctgagaagta gtttcgacga
     2401 tttggacttc agggatcggg cgagggacgg gagagccggc ttcgcccttg cacccgagca
     2461 ggccgaacac tcccattaaa agaacactgc ttaaggagag cctcctgcgg gtcgtctcat
     2521 t
//
LOCUS       DASTWE010000494         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1176745, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000494 DASTWE010000000
VERSION     DASTWE010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176745"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFT30_05180"
     CDS             complement(<1..760)
                     /locus_tag="VFT30_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5092056.1"
                     /translation="MSSRLLRTKPIDQILADADRPEHRLKKTLTAWDLTALGIGAIIG
                     TGIFVLIGTAIVGDAHRPGAGPGIVLSFILSGITCALAALCYAEFAAMIPVAGSAYTF
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFNKLLVLAGLELPYWATNPPHWA
                     GGPEGSIANFPAAIIVLMVTVLLVIGIKESARAAGLIVLLKLGVIVFFLAVGTPAVNT
                     DNWTPFMPNGFEGVRAAAAIIFFAYIGFDAVSTAA"
     gene            complement(892..>1146)
                     /locus_tag="VFT30_05185"
     CDS             complement(892..>1146)
                     /locus_tag="VFT30_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5092057.1"
                     /translation="SLLNKVVARRKLMVNSSHHQSVKTVAPSLKASAVAPDGIIEAIE
                     SPTHRFLLAIQWHPEFLFERHAVHRRLFEALLHAACRTRA"
BASE COUNT          285 a          315 c          329 g          217 t
ORIGIN      
        1 ctgcagcagt agagacagca tcaaacccga tatatgcaaa aaaaatgatg gccgcggcgg
       61 cccgaactcc ctcgaagccg ttgggcatga agggcgtcca gttgtcggtg ttgacagcgg
      121 gagtcccaac cgcgaggaag aagacgatca ccccaagttt gaggagcacg atcagaccgg
      181 ccgcgcgcgc actttccttg attccaatca cgaggaggac cgtgaccatc aatacgatga
      241 tcgcagctgg aaaattggcg atgctcccct caggcccgcc agcccagtga ggtggattgg
      301 ttgcccaata gggcagttcc aggcccgcga gcaccagtaa cttattgaaa taaccggacc
      361 agccaatagc aacggccacg cacgccacac catattccag aatcagattc caccccgtga
      421 gccaggcaag aaactcaccg agcgtggcat aagaaaatgt atacgcagac ccagcgactg
      481 ggatcatggc cgcaaattcg gcataacaca gcgcggctaa ggcacaggtg atgccggaaa
      541 gaatgaagga aaggacgatc cccggtccgg ctcccggcct gtgtgcatca ccgactatgg
      601 cggtaccgat caggacgaaa atgccggtcc caataatcgc cccgatccca agcgccgtaa
      661 gatcccaggc cgtcaacgtt ttcttgagac gatgctccgg acggtcggcg tcggctagaa
      721 tttgatcgat gggtttcgtt cggagaagtc ggctgctcac ggtctctctc gagcccaacg
      781 aaccaggatt cgctgtgacg ggttcaatgc gctggggctc catttaagga ccaagtatag
      841 cagggaccac gtgagtcggt tggttgtgat tgttggaacg gtccagagag atcatgcacg
      901 cgtccggcag gccgcgtgta gcagagcttc aaacagtcga cgatggaccg catgtcgctc
      961 gaacaagaat tctgggtgcc attggatagc gagtaaaaat cgatgagtgg gggattcgat
     1021 cgcctctata atcccatcgg gtgccacggc acttgccttg agcgacgggg cgactgtctt
     1081 gaccgattgg tgatgtgaac tattcaccat gagctttctt ctggcgacca ccttgttcaa
     1141 aaggct
//
LOCUS       DASTWE010000495         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_13672, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000495 DASTWE010000000
VERSION     DASTWE010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13672"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFT30_05190"
     CDS             complement(<1..743)
                     /locus_tag="VFT30_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5092058.1"
                     /translation="MAERPILRRRQWLLTRDFSLVWWSQVLSQVADGVSKLALLWFVY
                     SITGSALKTTVIGLLQTLPPIVLGPVIGVVVDRLPKKAILICSDLARSLLIGLIPCWV
                     SVESFTVQSLYVLTFLYGIATAMFIPTLSSSVPLMVKKEQLTAANALLQSTTSLGIII
                     GPVISGLGIAFSGSQDVLCINGVTYLASAAFLFPLRLSAGMTTSPPESRRTTAMEDLF
                     DGIRYTLISRRTILMLIMLASLYTFGSGA"
     gene            complement(918..>2116)
                     /locus_tag="VFT30_05195"
     CDS             complement(918..>2116)
                     /locus_tag="VFT30_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5092059.1"
                     /translation="WWGQMISQIGEGLNKVALLWFVYTLTGSALKMAVVGLIQTVPPL
                     LFGPMIGVFLDRLPKKTVMVWVDLIRTVMTFLIPALFALDLLSLEGLYVLIFLTSIVS
                     TIFGPALVSAVPRLVRPSELVTANALIQGTNNIGLLLGPAISGVLIAMIGAENVLYVN
                     SATFLVSAFCLIPMRFHEKLRTAAEVGNTSVSVLDDLKVGFRFVFGTHSTVFLLVVIS
                     ALYNLGVSAFVFILPVYAKEFLQVGPVQLGWLWSGLGVGMLAVSTWLAWRHQGDLHSR
                     LRLIVRGMTVGGLAVCSLSLLETPLVAAAVVIVVGGSTAVMNPVVWALLQEATPEHLM
                     GRVLTTFSTGSMATAMAGMTGFGWIADAVGPAQSLIGLGLVLLLTAAVAIRFMRQAVN
                     AQRVAA"
BASE COUNT          553 a          588 c          603 g          372 t
ORIGIN      
        1 aaggctccgc tgccgaacgt atatagggat gccagcatga tcaacatgag gattgtgcga
       61 cgagatatca atgtatagcg aataccgtcg aatagatcct ccatcgccgt tgtccggcgg
      121 ctctctggcg gagacgtcgt catcccggcc gatagacgga gggggaacaa gaatgccgca
      181 gacgccagat aggtcacccc attgatacag agcacgtcct gtgaaccgga aaaggcaatc
      241 cccagtccgc tgatgaccgg gccgatgatg atgccgagac tcgttgtgct ttggaggagg
      301 gcattggcag cagtcaattg ttcttttttc accatgagcg gcaccgagga tgagagtgtc
      361 gggatgaaca tggcggttgc aattccataa agaaacgtca gcacgtagag cgattggacg
      421 gtaaacgatt caacggatac ccagcaaggg atcaagccga tcaataggga cctggccagg
      481 tcgctgcaga tcaggatggc cttcttaggc aatcgatcga ccaccacgcc gatcacgggt
      541 ccgaggacaa tcggcggcaa tgtttgcaga aggcctatga ccgtggtctt cagcgccgaa
      601 ccggtgatgg aatagacaaa ccacaggaga gccaacttgc tgacaccgtc ggccacctgc
      661 gacagcactt ggctccacca caccaggctg aaatctcggg tgaggagcca ttgtctgcga
      721 cggaggatcg gacgttccgc catacgagac atctcctctt cctgattcat ccctgaggga
      781 ccggcctgac aggatgcgta tgccgattgt ctcccgcata cgtgacccag cttatcaggt
      841 tatatcggaa tggggccgac aagaaaggcg gaacggaggg tagaatgatt ggggcgcaac
      901 gcaacctgac gcgaggtcta ggccgcaacc cgttgagcat tgaccgcttg acgcataaat
      961 cggatcgcga cggctgccgt cagtaacaga accagaccga gtccgataag gctttgcgcc
     1021 gggccgacgg cgtccgcaat ccacccaaag ccggtcatgc cggccatggc ggtggccatc
     1081 gatcccgtgc tgaatgtcgt aagcacgcgt cccattaagt gttccggggt ggcttcctgc
     1141 agcaacgccc acacgacggg attcatgacc gccgtgctac caccgacaac aatgaccacc
     1201 gcagcagcga ccagcggtgt ttcgagcagg ctgagactgc agaccgccag tccacccacc
     1261 gtcatcccac ggacgattag gcgcaagcgg ctatgtagat ctccctggtg cctccaggcg
     1321 agccaggtgg acaccgccag catgccgacc ccgagaccag accagagcca gcccagttga
     1381 accggaccga cctggagaaa ttctttcgca tacacgggca gaatgaaaac gaaggcgctc
     1441 acacccaggt tgtacaaggc ggagataacg acgagaagaa aaactgtgga gtgggtgccg
     1501 aagacaaatc gaaatcctac tttgaggtca tcgagtacgg agacggaagt gttgccgacc
     1561 tctgcggcag tcctgagttt ttcatggaac cgcatgggaa tgagacagaa agccgacacg
     1621 aggaacgtcg cagaattcac atataggaca ttctctgcgc ctatcatcgc gatgagcacg
     1681 ccgctgattg ccggtcccag caacaaccca atgttattcg taccttgaat caacgcattc
     1741 gcggtcacca attcagacgg acggaccaat cgcgggacag ccgacaccag cgccggccca
     1801 aagatggtcg agacgatcga tgtcaaaaag atcaggacat acaggccttc aagggagagg
     1861 aggtcgagag caaataaagc cgggatgaga aaggtcatga cggtacgaat caaatccacc
     1921 cacaccatca cggtcttctt cggcaggcga tcaagaaaaa ctccgatcat ggggccgaac
     1981 agcaaaggcg gaacggtttg gatcaatccg acgaccgcca tttttagggc cgaaccggtg
     2041 agggtataga cgaaccagag tagcgcgact ttattgagac cttctccaat ttgagagatc
     2101 atttgtcccc accaga
//
LOCUS       DASTWE010000496         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1144640, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000496 DASTWE010000000
VERSION     DASTWE010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144640"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..776)
                     /locus_tag="VFT30_05200"
     CDS             complement(51..776)
                     /locus_tag="VFT30_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5092060.1"
                     /translation="METEQHARKNAILGAIPREEFHRLLRQMQTVEVEAKQVIYQAGQ
                     PVDQIYFPTTALMSMLMPTAEGETVELAMVGREGIVGVSALLNSGRSREYGTLAKVIT
                     LIPGNAVQIKSEVFESAMEKSLHISRCLRSYLGLLFTELSLSVSCNRLHSLEQRCARW
                     LLTAMDKSGGMQEVLVTQEMLAAILGVVRQSIFQVLNDFESKQIIKCGWGNIKVESRQ
                     RLAAAACECYGVLRKRLERMTAL"
     gene            1076..1465
                     /locus_tag="VFT30_05205"
     CDS             1076..1465
                     /locus_tag="VFT30_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5092061.1"
                     /translation="MKPMMILIIDDNSEDRSAYRRFLEPFGGYVIQEAEDGKMGLDMA
                     RQLLPNCILLGLKLQDQSGLEVLVKLMDEASTSTIPVIMISTGIAKVVVDAALSFGAS
                     HFLIKGKFDATALDAAIRESVARQTSP"
BASE COUNT          373 a          408 c          377 g          399 t
ORIGIN      
        1 ccaaatgaaa agaaactttt tgctgccggt ggcctggttg acgacgtcgg ttagagagcc
       61 gtcatacgtt ccaatcgctt cctcaagacg ccgtaacatt cacaggctgc cgccgccaag
      121 cgctgccgac tttcaacttt aatattgccc caaccgcatt taataatttg ctttgattca
      181 aagtcgttga gaacttgaaa aatactttgt cgcacaaccc ccaaaatcgc ggccagcatt
      241 tcctgcgtca cgaggacttc ctgcattccc ccgcttttat ccatggctgt cagcaaccat
      301 cgcgcacatc gttgttccag cgaatgcaat ctattgcagg agaccgacag cgagagctcc
      361 gtgaacagga gtcccaagta tgaacgaaga cagcgcgaga tgtgaaggct tttttccatt
      421 gcggattcaa acacttcgct ttttatttgg acggcattgc cgggaattaa tgtgatgacc
      481 ttagccagtg tcccgtactc gcgagatcgg ccgctgttca agagcgcgct taccccgacg
      541 attccttcac ggccgaccat ggcaagttcg actgtttcac cttccgctgt cggcatgagc
      601 atcgacataa gggcggttgt cggaaaatag atttgatcga cgggttgacc tgcctgatag
      661 atgacttgtt tggcctcgac ctcgaccgtc tgcatctgcc gaagcagtcg atgaaactct
      721 tcacgtggaa tcgcgcctag gatggcattc ttacgggcat gctgttcagt ctccactagg
      781 gactcccctt tatcggccgg cccgcagtgt gcctcagaac caaggtggga gtctgtcgtc
      841 tgggctacaa ttgctcagtt caacatgaaa accgggtgct gctcagtaaa tttttctaga
      901 tattgtctgg cacttgtcgc ctacccgaca gacttaacat tctcgagtga ttaagatgtg
      961 cagacttgac atcactattt acgtcctatc tagccaatgt cctcgcgttt gccaggctca
     1021 ttcttgacat atatcaatgg cgagccaacg gactttcaga caatgagcgt attgtatgaa
     1081 accgatgatg attttgatca ttgacgacaa ttccgaggat cgatcggcat atcgccgttt
     1141 tctggaaccg ttcggaggct atgtcatcca agaagccgaa gatgggaaga tgggattgga
     1201 catggcgcgt caactgctcc cgaattgcat actgttaggt ctcaaactcc aagatcaatc
     1261 agggctggag gttctcgtca agctcatgga cgaggcctca acatcaacca taccggtgat
     1321 catgatcagc accggcatcg cgaaagtcgt ggtagatgcc gcactctctt tcggtgcttc
     1381 ccattttctt atcaaaggca aatttgatgc gacggcattg gatgccgcga tacgagaatc
     1441 agtagcaagg cagacaagtc cttgatgccg atgttgaaca gtacgcagga gaggtggttc
     1501 cggaacagcg gtgctgctct ttttcactca tttcaatagc caaccaactt ttacgcc
//
LOCUS       DASTWE010000497         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1597231, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000497 DASTWE010000000
VERSION     DASTWE010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597231"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1295)
                     /gene="tuf"
                     /locus_tag="VFT30_05210"
     CDS             complement(108..1295)
                     /gene="tuf"
                     /locus_tag="VFT30_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5092062.1"
                     /translation="MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITKVLAKNNPKVQ
                     FVAFDQIDKAPEEKARGITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAMVVFMNKADAVDDPELLELVELEVRELLS
                     AYEFPGDDIPVVKGSALKALEGDPEWEKTVIELMAAVDSYIPTPERDIEKPFLMPVED
                     IFTIQGRGTVATGRVERGKVKVNETVEIVGIRPTRSTVVTGVEMFKKLLDEGVAGDNV
                     GLLLRGIDRKDIERGQVISKPGSITPHTKFKAEAYVLTKEEGGRHTPFFSGYRPQFYF
                     RTTDVTGVATLPAGVEMVMPGDNVAMEIELIAPIAMEKGLRFAIREGGRTVGAGTVSD
                     VIE"
BASE COUNT          252 a          477 c          292 g          333 t
ORIGIN      
        1 gactggtcca agacgcgatg gtcgtaagcc ttaagtcgga ttctaatttt ttcgttcatt
       61 cttggccttt agtcttccta acgtcctatt gataggtcct ataggtccta ttcgatcaca
      121 tcagataccg taccggcgcc aaccgtgcga ccaccttcgc gaatagcgaa gcgcagtccc
      181 ttctccatcg caatcggcgc gattaactca atctccatcg ccacgttgtc gcccggcatc
      241 accatctcca ctccggccgg caacgttgcc actcccgtca cgtccgtcgt gcggaagtag
      301 aactgcggcc gatagccact gaagaacggc gtatggcgcc cgccttcttc cttcgtcagc
      361 acatacgctt ccgccttgaa cttcgtgtgc ggcgttatgc tgcccggctt cgagattacc
      421 tggccccgtt caatgtcctt gcggtcgatt ccgcgcagca acaacccaac gttgtcaccc
      481 gccacgcctt cgtccagcaa cttcttgaac atttccacgc ccgtcactac cgtcgaccgc
      541 gtcgggcgaa tgcccacgat ttccaccgtc tcattcacct tcaccttccc gcgctccact
      601 ctccccgtgg ccaccgtgcc gcgtccctga atcgtgaaga tgtcttccac cggcatcaag
      661 aacggcttct caatatcgcg ctccggcgtc ggaatgtagc tgtctacggc ggccatcaac
      721 tcgatcaccg tcttctccca ttccgggtct ccttccaacg ctttcaacgc cgaccccttg
      781 actaccggaa tgtcgtcgcc cggaaactca tacgcgctca acagctcgcg cacttccagt
      841 tccaccaact ccagcaactc cggatcgtcc accgcatctg ccttgttcat gaacaccacc
      901 atcgccggca ctcctacctg ccgcgccaac aggatgtgtt cccgcgtttg cggcatcggc
      961 ccatcggtcg cggccaccac caggatcgcc ccgtccatct gcgctgcgcc cgtgatcatg
     1021 ttcttcacgt agtcggcgtg tcccggacag tccacgtgcg catagtgtcg attcgccgtc
     1081 tcgtattcca cgtgcgccgt cgcaatcgtg atacctcgtg ctttctcttc cggcgccttg
     1141 tcgatctgat cgaaggccac aaactgcact ttcggattat tcttggccaa caccttcgtg
     1201 atcgctgctg tcaacgtcgt cttaccatgg tccacgtgac caatcgttcc cacgtttacg
     1261 tgcggcttag ttcgctcaaa tttctctttt gccacggctt gagtcctcta aaactcgaat
     1321 tggttactaa aactgaaatc tcaaaaaatc taca
//
LOCUS       DASTWE010000498         1366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_563316, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000498 DASTWE010000000
VERSION     DASTWE010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563316"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFT30_05215"
     CDS             complement(<1..782)
                     /locus_tag="VFT30_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEU5092063.1"
                     /translation="MEEDWKQVLAKSIVKPKDLAERFGLDEKEIEAIVGPYPMRITPT
                     VLETIKSKDDAIWRQVVPDAAELDDIAADDDPLEEDLMSPVPHLVHRYPDRVLLMVTN
                     QCPIYCRFCTRKRLVGKPGFLKKGELDRAIQYLREHKEVRDVILSGGDPLLLPDHLLE
                     RVLKALRTIPHLELIRIGSRVPGSLPQRITPKLCEMVKKYHPIYMNLHFNHPDELTPE
                     VKAACGMLADAGIPLGAQTVLLKGVNDDPEVMKRLVHQLLLAR"
     gene            complement(800..1138)
                     /locus_tag="VFT30_05220"
     CDS             complement(800..1138)
                     /locus_tag="VFT30_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5092064.1"
                     /translation="MAVTLFHVDWCPDCHVVRRKLADLGVTYEAIVVPDSRRMRTQVY
                     EVSGQYYVPVLKDGDLILTETADILAYLDERYSADRVGTPTATEFQSQARTTPEVSGK
                     DDEHPSCRIN"
BASE COUNT          298 a          369 c          387 g          312 t
ORIGIN      
        1 cgcgcaagta gtagctgatg gacgaggcgt ttcatgactt ctggatcgtc gttcacgccc
       61 ttgagaagaa ctgtttgagc gcccagagga atgccggcat ccgcgagcat gccgcaggcg
      121 gctttgactt ccggtgtcag ctcgtctgga tggttgaaat gcaagttcat ataaatggga
      181 tggtattttt ttaccatctc acacagtttg ggagtgatcc gttggggcaa cgatccgggg
      241 acccgcgagc cgatccgtat gagttccaag tggggaatcg ttcgaagcgc cttcaacaca
      301 cgttcgagga gatgatcggg gagcaggagc gggtcgcctc ccgagaggat gacatcgcgc
      361 acttctttgt gctcgcgaag atactggatg gcgcggtcca attctccttt tttgaggaaa
      421 ccgggcttgc cgaccaatcg ctttctggta caaaaccgac aatagatcgg gcattgattc
      481 gtcaccatga gcaacacccg gtccggatag cggtgaacga ggtgaggcac cgggctcatg
      541 agatcttctt caaggggatc gtcgtcggcc gcaatgtcgt ccaactctgc agcgtctggg
      601 accacctgcc tccaaatcgc atcatccttt gatttgattg tctcgagcac ggtcggagtg
      661 atccgcatgg gataagggcc gacgatcgct tcgatctctt tttcatcgag accgaaccgt
      721 tcggccaagt ccttgggctt cacgatgctc ttggccagga cttgtttcca gtcttcttcc
      781 atgaatcgtc cttcgttgat caattgatcc ggcaagaagg gtgttcgtcg tctttgccag
      841 aaacctcggg cgtggtgcgg gcctgcgact gaaactcagt tgctgtcggg gttcctacgc
      901 ggtcggcact atatcgctcg tccagatagg ccaaaatgtc ggcggtttcc gtcagaatga
      961 ggtcgccatc cttgaggacc ggtacataat attggccgga gacctcgtaa acctgcgtgc
     1021 gcatccgtct gctgtcgggt acgacgatag cttcataggt cactcccaaa tcagccagct
     1081 ttcggcggac cacgtgacag tcagggcacc agtcgacatg gaaaagagtc acagccatga
     1141 tggtataagc taacaggaaa tggaagaaag tggaacgccc attttggcgg gatgttgaaa
     1201 aagtcctcca gcttcgttct cgcaagacag aaccgcctca ccaagacact cggcgctcac
     1261 gggacacggc ccgctcaccg tctcgcgggc gttcacaaac gtgctgcgcc ttatttggcg
     1321 cggcgtgaac ctcagaagct caacataccg aacagcatgc acctag
//
LOCUS       DASTWE010000499         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1500309, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000499 DASTWE010000000
VERSION     DASTWE010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500309"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1145)
                     /locus_tag="VFT30_05225"
     CDS             complement(<1..>1145)
                     /locus_tag="VFT30_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5092065.1"
                     /translation="VPLPILDLSDSLDPKQAAELFCKREASAELDLVCGPLVKFVLIR
                     LRADEHWLLHVAHHINCDGASWDLYVEELGLLYEAKLRGDAPPLPEFTSPQYGDYATW
                     QRRLLSRAWPAYQTSVEWWRKYLSGASSTLELPFRRVHPQLEVNPSEGQFYWGIEPEI
                     SRGLNVVASAERATPYMVRLAAFAAILADVSGQPDVVFGGYISGRNRPELQRMLGDFS
                     NLVTLRFQYKPQLSFFEWLSKVRDTMLAVQKHSLIPYEKLCDELREIFQTPPQITALF
                     HMTQRYIPNKLANLEFTLLRRCEPVMPWGITVTHDGNDEVQECRVTFDANIHDPAGVR
                     LFVDRYTRLLDAISHHPNCSLAQLMTMSQTGGRLDGLIGPSQQCPGW"
BASE COUNT          262 a          313 c          319 g          251 t
ORIGIN      
        1 tccacccggg acattgttgc gaaggcccga tgaggccatc aaggcggccg cccgtctggc
       61 tcatcgtcat caattgcgcc agagagcaat tcggatggtg cgagatggcg tctagcaggc
      121 gggtgtaccg atcaacgaaa agacgaaccc cggcagggtc atggatgttg gcatcgaatg
      181 tgactctaca ttcttgcact tcgtcgttgc cgtcgtgcgt cacggttatt ccccatggca
      241 tgacaggctc gcacctgcgt agcagggtga actccaaatt ggccaatttg tttggaatgt
      301 atcgctgggt catatgaaac aatgccgtga tctggggtgg ggtttgaaat atttcccgta
      361 gttcgtcaca taatttctcg tatgggatca agctgtgctt ctgaacagcc aacatcgtgt
      421 ctcgaacctt ggaaagccat tcgaaaaacg acaattgcgg cttgtactgg aatcggagcg
      481 tcaccagatt tgaaaaatct cccaacattc tctgcaactc gggccgattc cggccggaga
      541 tatagcctcc gaatacgacg tcgggctgtc cagacacatc tgcgagtatc gcagcaaagg
      601 cggcgagccg caccatgtag ggagtcgccc gttcggcact ggccactacg ttaagcccgc
      661 gtgagatttc cggctcgatg ccccaataga actgaccttc gctgggattc acctctagct
      721 gtggatgaac tcgcctaaag ggcagttcca gcgtgctcga cgctccggag agatacttcc
      781 gccaccactc aacggatgtc tggtatgctg gccaagcgcg actcaacagt cgtcgctgcc
      841 aggtggcata gtcgccgtat tgcggtgacg tgaactctgg caaaggaggg gcatctccgc
      901 gtagtttcgc ttcgtacagc aaacccaact cctcaacgta caggtcccag gacgcgccat
      961 cgcagttgat gtggtgagcg acatgcaaga gccagtgttc gtctgcccgt aagcgaatga
     1021 gcacaaattt caccaagggc ccgcacacga ggtccaattc ggcagacgct tcccgtttgc
     1081 aaaaaagttc ggcggcttgt ttcggatcga gcgagtcact tagatcgagg attggcaaag
     1141 gaacc
//
LOCUS       DASTWE010000500         1244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_886345, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000500 DASTWE010000000
VERSION     DASTWE010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886345"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFT30_05230"
     CDS             <1..287
                     /locus_tag="VFT30_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092066.1"
                     /translation="NVTERHALEPAIKFPAPRFVPYEMLVKFKGGVSQERIVSILKDN
                     RTDVIAEIQRGRLYHVRILDDRSVKSAITQLTSYQEVEYAEPNYRYETQK"
     gene            250..>1244
                     /locus_tag="VFT30_05235"
     CDS             250..>1244
                     /locus_tag="VFT30_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5092067.1"
                     /translation="MPSPIIGTKRKNSVTRIVLCWFGAVLVITTAVVVSSGLMPGDRF
                     VYAKHKQTSGGMTALLGDVRRSAHYAPGEVLVKFRDEASSSGIKSLNANMGAKELSAL
                     SVNAAVVHQYKLDGAMSVDEAVLKYRNNPAVEYAEPNYLYSLQAIPADAQFGTLWGLH
                     NTGQAVNGTAGRADADIDAPEAWDISTGSPNVIVAVIDSGIAYDHPDLAPNIWTNSGE
                     IAGNGVDDDGNGLVDDMHGWDFLMNDSDPMDPVDLNPGGNEGHGTHVAGTIAGAGNNG
                     TGITGVMWTARLMALKAGGVNNLLSTTAIISAIHYAIAKGARVINASFIGPECSLA"
BASE COUNT          312 a          307 c          356 g          269 t
ORIGIN      
        1 ggaatgtgac agaaagacat gccttagaac ccgctataaa attccctgca ccgcgattcg
       61 tcccctatga aatgttggtc aagttcaagg gcggggtttc acaagagcgg attgtgtcca
      121 tcctgaagga taaccgaacc gacgtgatcg ctgaaatcca gcgggggcgc ctttatcacg
      181 tccggatttt ggatgatcgg tcggttaaat ccgcgatcac ccagctcact tcatatcaag
      241 aggtcgagta tgccgagccc aattatcggt acgaaacgca aaaatagcgt gacgagaatt
      301 gtgctctgtt ggttcggggc tgtcttagtc atcacaacag ccgtggtcgt atcgtctgga
      361 ctcatgcccg gagatcgctt cgtgtatgcg aagcataaac agacatccgg tggaatgacg
      421 gcgttattag gggacgtgcg tcgctctgcg cactatgccc caggagaggt actcgtcaag
      481 ttcagggatg aggcgtcctc atcgggaatc aagtctctga atgccaatat gggtgccaaa
      541 gaacttagcg cgctatcggt gaatgccgcc gtggttcacc agtataaatt ggacggtgcc
      601 atgtcagtgg acgaagcggt cttgaagtac cggaataacc ccgccgttga gtatgccgaa
      661 ccaaactatc tgtactcgct acaagccatt cccgctgacg ctcagttcgg cacattatgg
      721 ggtctccata acacaggtca agctgtgaac ggaacggcag gaagggcaga tgccgacatt
      781 gatgcgcctg aagcctggga tatcagcacg ggaagcccaa acgtgattgt cgccgtcatc
      841 gactcgggca ttgcctatga ccatcctgat cttgcaccca atatctggac gaattcggga
      901 gagattgctg gaaatggcgt agatgacgat gggaatggat tggtcgacga tatgcatggg
      961 tgggattttc tcatgaacga cagcgacccg atggaccctg tcgatctcaa tcccgggggg
     1021 aacgaaggac acggcactca cgtcgcagga accatcgctg gagccggaaa caacggaaca
     1081 ggaatcacag gggtcatgtg gaccgcgaga ctgatggcgt tgaaagctgg gggtgtgaat
     1141 aacttattga gcaccaccgc cattatcagc gcgatccact atgccatcgc caaaggtgca
     1201 cgcgtgatta atgcgagttt tatcggacct gaatgtagcc tggc
//
LOCUS       DASTWE010000501         1154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_757291, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000501 DASTWE010000000
VERSION     DASTWE010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757291"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VFT30_05240"
     CDS             complement(<1..1144)
                     /locus_tag="VFT30_05240"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU5092068.1"
                     /translation="MDATLRIIEAAGAPLEIERIDIGEKVYLAGNSSGIEPSSWESLR
                     RTKVFLKAPITTPQGGGYKSLNVTVRKVLGLYANVRPCVSYHPFVETKHPKMDVVIVR
                     ENEEDLYAGIEYRQTADVMQCLKLISRPGSEKIIRYAFEYARQNNRKKVTCFTKDNIM
                     KLTDGLFHKIFDEIAKEYSDIENEHWIVDIGAAKLADTPEAFDVVVMPNLYGDILSDV
                     AAQIAGSVGLAGSANIGEHVAMFEAIHGSAPRRAGQNLANPSGLLLGSVMMLVHIGLN
                     EHAEKVHNAWLRTIEDGVHTYDIFDENVSKQKVGTREFADAVISRLGQVPQILKSAKY
                     LRSEGGVPKASVASASKTSQKELVGVDVFLDWNAGSASDLGQGLTKV"
BASE COUNT          255 a          316 c          276 g          307 t
ORIGIN      
        1 tcactttggt gagcccctgg cccaggtcgc tcgccgaccc ggcattccag tcgagaaaaa
       61 catcgacgcc aacaagttcc ttttgagaag tcttgcttgc gctagcaaca ctggcttttg
      121 gcacgccgcc ttcagatctc aaatatttcg ccgattttag aatctgtggc acttggccca
      181 atcgtgaaat aaccgcgtcg gcgaattctc gtgtccccac cttctgcttg cttacatttt
      241 catcgaagat gtcgtacgtg tgaacgccat cttctatcgt tctcaaccag gcgttgtgca
      301 ctttttccgc gtgttcattc aagccgatat ggaccaacat catcacgcta cccaacaaca
      361 agcccgatgg gttcgccagg ttctgaccgg ctcgtcgtgg agcggagcca tggattgctt
      421 caaacatcgc gacgtgctcc cctatgtttg cagaccctgc caggccaacc gaaccggcta
      481 tttgggccgc aacgtctgaa aggatgtcac cgtagagatt tggcatcacg acgacgtcga
      541 aggcttcagg agtatcagca agcttggccg ctccgatgtc cacgatccaa tgctcgtttt
      601 cgatatcaga gtattccttt gcaatctcgt caaagatctt atggaaaagg ccatcggtca
      661 gcttcatgat attgtctttg gtgaagcagg tgaccttctt ccgattgttc tggcgcgcgt
      721 attcaaacgc gtaccgaata atcttttccg accctggccg tgagattagc ttgagacact
      781 gcataacgtc tgcggtctga cggtactcaa tgccggcata aagatcctct tcattttcgc
      841 gcactatcac gacgtccatc ttgggatgtt ttgtctcgac gaacgggtga taagaaacgc
      901 atggacgaac gttggcgtac aagccgagca ccttgcgaac ggtgacattc aggcttttgt
      961 atccaccacc ttgaggagtt gtgataggcg ccttgagaaa aactttagtt cgtctgagag
     1021 actcccagga gcttggttcg ataccagatg aattgcctgc tagatagacc ttctcaccga
     1081 tatcgattct ttcgatttca agtggcgctc ctgccgcctc gataattcgc agcgtggcat
     1141 ccatgatttc tggc
//
LOCUS       DASTWE010000502         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_368907, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000502 DASTWE010000000
VERSION     DASTWE010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368907"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VFT30_05245"
     CDS             complement(<1..1130)
                     /locus_tag="VFT30_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092069.1"
                     /translation="MNLRGTVRVMPGVAGYDMVLSDMTAHLNNITLKGKANLAGLLTP
                     QPTFAVTFSSSLVALPQLLKTIPPDWIHPQLPALLADRQIDGKVQVVNATLTGSATAG
                     PQLSTIGEFHVSEGRGLIGGDRIAAKDLTAVVTVETGRVRITKVTGMYGAMHITGGKA
                     EVSFPNAGPWLELEITGEMGATHLIEFLTKTVKAERARQLLAGIRDAEGTAQSTFRLL
                     GPLNQSGGITFAGGEITARQVSLTHVNLPERLTGMQGRLVFADGSTQFDQVTGHLSGT
                     AVQVQGAITGGVTSQFQDFVIRIRGDAAQIVRLFRFTGGEHGMLDGTLSTEVSLSGST
                     TRPQVRGSVVLDEAKVVLGSIEKPVGAHATVEFEGSMPKEAGV"
BASE COUNT          267 a          375 c          322 g          288 t
ORIGIN      
        1 acgcccgcct ccttgggcat actgccctca aactccaccg tcgcgtgggc tccgacgggt
       61 ttttctattg acccaagcac tactttcgct tcgtcgagca cgacggatcc acgaacctgg
      121 ggtctcgttg tcgagccgga cagggaaacc tccgtgctga gcgtgccatc gagcatgcca
      181 tgttcgcccc cggtaaaacg gaacagcctc actatctgtg ccgcatcacc gcgtatgcga
      241 atcacaaagt cttgaaattg acttgtcaca ccgccggtga tcgccccttg gacttgcaca
      301 gctgttccac tgagatgtcc agtgacctga tcaaactgtg tggacccatc ggcaaaaacg
      361 agtctcccct gcatcccggt caaacgctcc ggtaaattca catgggtgag actgacttga
      421 cgagccgtaa tctccccacc ggcaaacgtg atccctccgg actggttcaa cggtcccagc
      481 agacgaaacg tcgactgggc tgttccttct gcatcacgaa taccggctaa caactgcctg
      541 gctcgttccg ctttcaccgt cttcgtcaaa aattcgatga gatgcgtggc ccccatctct
      601 cccgtgatct ccaattcgag ccatggacct gcattgggaa acgatacctc tgcttttcca
      661 ccggtgatat gcatcgcccc atacattccg gttaccttcg taatgcgaac acgtcctgtt
      721 tctactgtca ccactgcggt gaggtctttc gcagctatac gatcaccgcc gatcaagcct
      781 cgaccttcgc tgacatgaaa ctctcctatc gtcgagagtt gggggccggc cgtcgcagac
      841 ccagtgagcg tcgcattcac cacttgcact ttaccgtcga tctggcgatc agccagcaga
      901 gctggaagct gcggatggat ccagtcaggg ggaatggttt ttagtagttg agggagggcg
      961 acaagagaac tactgaacgt gacggcaaac gttggctgag gagtcaggag gccggctaga
     1021 ttggccttcc ctttcagggt gatattgttc aaatgggcgg tcatgtcgga taggaccatg
     1081 tcatatccgg caacacccgg catgacgcgg actgtacccc tgaggttcaa cgctccttga
     1141 agttgctcag aaaccggtct gggaccgaga aagtctgcta cgtcccgtat cttaagatcg
     1201 gcggcatcga tctgtccttc aaactgaaat cctgtggccg attcggttgt gc
//
LOCUS       DASTWE010000503         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1532633, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000503 DASTWE010000000
VERSION     DASTWE010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532633"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFT30_05250"
     CDS             <1..963
                     /locus_tag="VFT30_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092070.1"
                     /translation="LGIVGVNLLYAAFYFRDKPQLLLKSLLDNLSTERVEIDMAEFSG
                     NEFKHVDNRAVSLKLVQLGLSGAAMFGPSGDVLQPSEVLRKKPVLVERGSFRPVTKVN
                     IDIIDCALKQFSADLAVAPDGVVELMEINLRQLMVNGTVDLGDFLARADLLEATGKTV
                     LISDYFEYYRLATYLARYTSEPIAVAMGVPSLMDLFEEEHYSDLDGGILEAFGKLFTK
                     NMRIYVYPYRDPTSGVLETADTVTIPDEQRNIFEHLVERGRIKAIRNFDESILHIFSR
                     DVLQRIKDGDTLWETMVPQEITAIIKAHRLFGYRADEANAARPV"
     gene            960..>1249
                     /locus_tag="VFT30_05255"
     CDS             960..>1249
                     /locus_tag="VFT30_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092071.1"
                     /translation="MTVGKGLMVVVLFSWPVAPDLSAQAPSAPTLEQFLEASVVPREV
                     IDRFLRGPSWAQIDPELGYIPGSFLPADGMDKSSSISTVQSTGARTSFLYAD"
BASE COUNT          267 a          328 c          360 g          294 t
ORIGIN      
        1 ctaggtattg tgggtgtcaa cctcctctat gccgctttct actttcgcga taagccccag
       61 ctgttactca aatcgttact cgacaactta agtacagagc gtgttgagat cgacatggct
      121 gagttttccg gcaacgaatt caaacacgtc gacaaccgcg ccgtgagcct gaagctggtg
      181 caactgggtt tgtcaggggc cgcgatgttc ggcccgtcgg gcgacgtgct gcaaccctct
      241 gaagtgcttc gcaaaaagcc agtattggtg gagcggggta gctttcgtcc agtcaccaag
      301 gtcaacattg acattatcga ttgcgctctc aaacagttct ccgcagacct tgcggtggcg
      361 ccggacggcg tcgtggagct tatggagatt aatctgcggc aacttatggt aaatggcaca
      421 gtcgatcttg gcgacttcct ggcgcgggcc gacctactgg aggctaccgg caagacggta
      481 cttatctcgg actacttcga atactatcgg cttgccacgt atctggcgcg ctacacgtcg
      541 gagccgatcg cggtcgcgat gggtgttccg agtcttatgg acttgtttga ggaggagcac
      601 tacagcgatc tcgatggcgg catcttggaa gcctttggca agctcttcac caagaatatg
      661 cggatctacg tctatcctta tcgtgacccc acttcgggtg ttcttgaaac cgcggacacc
      721 gtaaccatcc ccgacgagca gcggaatatt ttcgagcatc tggtggagcg gggcaggatt
      781 aaagcgatcc ggaacttcga tgaaagcatt ctgcacatat tctcacgcga cgttctgcag
      841 cgcatcaagg atggcgacac tctctgggag acgatggtgc cccaggagat taccgcgatt
      901 atcaaggccc atcgcctgtt cggctacagg gcggatgaag cgaatgcagc gaggccggta
      961 tgacagtggg aaaaggattg atggtagtgg tcttgttctc atggccagtt gcgccagatt
     1021 tgagcgctca ggctccaagc gcacccacct tagaacagtt cctcgaggcg agtgtcgttc
     1081 cgagggaagt gatcgatcgg tttctccgcg gcccctcgtg ggcccagatt gacccggaac
     1141 ttggctatat acctggaagt ttcctgccgg cggacgggat ggacaagagt agttcgatct
     1201 caacagttca atctaccggg gctcgcacgt catttcttta tgcggatag
//
LOCUS       DASTWE010000504         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_757322, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000504 DASTWE010000000
VERSION     DASTWE010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757322"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1070)
                     /locus_tag="VFT30_05260"
     CDS             complement(<1..>1070)
                     /locus_tag="VFT30_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092072.1"
                     /translation="LFSAEVPYFVEALVLVIFVLYVVLKGPRPESISLEALKKAGIDE
                     RQFSNAIESEKRFFSYWPVAWVLWFFLYVALGTESAVSKSDLPPYVMALYPVLSNVLN
                     SATCIPFVGMYYELSENTTECPSKFVAQRRWFWFLLSFIAVTAAIESGLRVFLPDAAT
                     EAGYWASLISGCLVGVCIGLFVFKLLSLRLFDLPLWTILLLTLYVVVQPVFPAVTAPA
                     DDRVGYALVLVALYAKIVLLVVVEWMRDTYRILYFMLRSAEISDDGKVHEEFAQTATD
                     AFGPPTGPSEIFKKLFAPAAGIVFLAVFVTELVEIAKGESPSLLKQAMPFIEAFCLAA
                     FAFYAVFKGDREEKPHLERQFR"
BASE COUNT          293 a          295 c          287 g          195 t
ORIGIN      
        1 gacgaaattg tcgttccagg tgaggctttt cctctctgtc gcccttgaag accgcgtaaa
       61 aggcaaacgc tgcgagacag aacgcttcta tgaacggcat ggcctgtttc agaagactcg
      121 ggctttctcc tttggcgatc tcaaccaatt cggtaacgaa gaccgccaga aacacaattc
      181 ccgcggcagg agcgaacaat ttcttgaaga tttcggacgg acccgtcggc gggccgaaag
      241 cgtccgtcgc tgtctgcgca aactcttcat ggactttccc gtcgtccgaa atctccgccg
      301 accgcagcat gaaatacagg atgcgatacg tgtctctcat ccattcgacg acgacaagca
      361 gtacgatttt cgcatagagc gcaacgagca cgagcgcgta accgacccga tcatcggcag
      421 gtgccgttac ggccgggaag acgggttgca ccaccacata aagcgtcaga agcagaatgg
      481 tccacagggg caaatcgaac agccggagac tcagaagctt gaatacaaat agcccgatgc
      541 acacgccgac gagacatcct gaaataagag atgcccaata tccggcctct gtcgcagcgt
      601 ccggaagaaa caccctcaga ccggactcga tggcggcggt tacagcaatg aaggacagca
      661 ggaaccaaaa ccaccgccgc tgtgcgacga acttggaagg gcactcggtc gtattttcag
      721 agagctcata gtacatccca acgaatggga tgcacgtcgc gctattgagg acgttggaaa
      781 ggacgggata gagcgccata acgtatggcg gaaggtccga tttagacacc gcgctttccg
      841 tcccaagcgc gacatagaga aaaaaccaca acacccaagc gacgggccaa taggagaaga
      901 agcgcttttc gctctctatg gcattgctga attgccgttc gtcgatgccg gctttcttga
      961 gagcctcaag ggagatgctt tcgggccggg gccctttgag aacgacatag agaacaaaaa
     1021 ttacgagcac caacgcttcg acaaagtagg gcacttcggc tgaaaacaac
//
LOCUS       DASTWE010000505         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_142972, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000505 DASTWE010000000
VERSION     DASTWE010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142972"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFT30_05265"
     CDS             complement(<1..416)
                     /locus_tag="VFT30_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEU5092073.1"
                     /translation="MKVLMIGDVVAKPGRIAVLERIQDLREQHQIDLAIMNAENLAGG
                     FSVTPSLCEQLFATGIDVMTSGNHIFDKKEAVDYIRKQPRLLRPANYPPNTPGSGYWL
                     GEVKGVKVAVLNVMGRVFMPPVDDPFRTIDHLLNGLP"
     gene            complement(517..704)
                     /gene="ssrS"
                     /locus_tag="VFT30_05270"
     ncRNA           complement(517..704)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFT30_05270"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(844..1233)
                     /locus_tag="VFT30_05275"
     CDS             complement(844..1233)
                     /locus_tag="VFT30_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU5092074.1"
                     /translation="MSSGIGRMINKGSMEATSSSPTIRVEIYNQTYNIRSDGDTEYII
                     QLAEFVDGRMREISSGTLTVDSLKVAILAALHIADELHRLKNMHEQADAQLGARSAEC
                     AEMLDRLLKVRSTLEHEQGGAGKLSLG"
     gene            complement(1230..1508)
                     /locus_tag="VFT30_05280"
     CDS             complement(1230..1508)
                     /locus_tag="VFT30_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092075.1"
                     /translation="MVGLSGLEKFSHLEDKIYRTIELTKTLRQEKENLEKELSLIHRD
                     MGNVLNEKERLESQVEKLLAERETIRMKVEAMLDAVAALDPEMAEELR"
BASE COUNT          351 a          418 c          454 g          399 t
ORIGIN      
        1 ggtaagccgt tcagcaggtg atcgatcgtg cgaaagggat cgtcgactgg cggcatgaat
       61 acgcggccca tgacgttgag cactgcgacc ttcacgcctt ttacttcgcc gagccagtag
      121 ccgctgccgg gagtgtttgg cggatagttc gcggggcgca acaggcgcgg ttgtttacgg
      181 atgtagtcga cagcttcttt cttatcgaag atgtgattac cggacgtcat gacgtcgatg
      241 ccggtggcga agagttgttc gcagagcgac ggagttacgg agaagccgcc ggcaagattc
      301 tcggcattca tgatggcgag gtcgatctga tgttgttcgc ggagatcctg gatgcgctca
      361 agcacggcaa tacggccggg cttagccacc acgtctccga tcatgaggac tttcatagtg
      421 gtgtctggga caataaaaaa gccgcgattc acggatcaga agagcgtgac aaacgaatca
      481 cgatctacga ttcacgattc gcggtacctg gcaggaggga gatccacgtt tgccgttccg
      541 aagccgtaat tgaacctgca tagacaggtg ggtgacctct taatgacgca gcctctggca
      601 aatcttaacg atgaagactt gcactggcta ggcgacctcg gtctcccgaa tcaacggcgt
      661 tggctcaatc atcgtacact cggtgacgaa caccgcagaa cgtttccctc cgccgcgacg
      721 gatagtaaca caagaccctt cgatcttagt actttgtgtt ttgtacttgg tacggaggtg
      781 gctggttaaa cgttattcgg agcttctgca aacgaacaaa gtacaaagca cgaagtacaa
      841 agctcatccc aaggagagct taccggcgcc gccttgttcg tgttcgaggg tggagcggac
      901 cttcaataac cgatcgagca tttcggcgca ttcagcgctt cgggcgccga gttgggcatc
      961 ggcttgctca tgcatgttct tgagacgatg aagctcgtcc gcgatgtgca gtgcggcgag
     1021 aatcgcgact ttcaatgagt cgacggtaag tgtgccagag gagatctcgc gcatgcggcc
     1081 gtcgacgaat tcggcgagct ggatgatgta ttcagtgtcg ccgtcagacc ggatgttgta
     1141 ggtctgattg taaatctcaa cacggatggt gggactgctg ctggtggctt ccatgctgcc
     1201 tttattgatc atgcgaccga tcccggaact catctgagct cctcagccat ctccggatcc
     1261 agagcggcca cggcatcgag catcgcttcg actttcatgc ggatcgtctc acgttcggcg
     1321 agcaacttct cgacctgact ttcgagtcgc tctttttcat tcaaaacgtt acccatgtcc
     1381 cgatggatta gcgacagctc tttctcgagg ttctcttttt cctgacgcag ggttttggta
     1441 agttcgatgg tccgatagat cttatcttcc agatgcgaaa acttttccaa gcctgacagg
     1501 ccaaccatct tttgtctccc gtggacgttt cgactgacgt gaaatcgaca gcgattatcc
     1561 gtcttcgcgg ttgcaagcgc aagactcatt tcacaatccg aggcgattta ttttgctgct
     1621 gt
//
LOCUS       DASTWE010000506         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1564976, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000506 DASTWE010000000
VERSION     DASTWE010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564976"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          259 a          309 c          325 g          295 t
ORIGIN      
        1 caaaatcgat ctagggggaa aaacactgga ccagtatttg gaacaactcc gtattgccgt
       61 ctcgtttcgt gagcgggaag gacccacgat acgagctact ctagtcagct cgctacaggc
      121 gtgagacgca gggaatgtag catgcttaaa tatcacgcga aggcactcat ttaggaatcg
      181 cccggcgcgc catctccggg gtgaagcgca agcagggaac ctctcccgca tgtgccgttt
      241 ctgatgacat caagacggac ggccaccgcc ccggcgcaag ggttaccgct tttcggacgc
      301 gtcgtcatcg gatcgttttg ggatttctct tcattccaat tgcactgttt gcgtatttca
      361 agaatagcta tttcggagcc gatccatcct ggcggacgat cgagacggct aaattcgcat
      421 caagttaacg ctctactcct gacctgtgct gccatcgctg tgctgggatg ggttatgtgg
      481 tgatttgtcc ataacgttgt gcggcagaac tcataggatc agaaagaaag agcttggaag
      541 tcgctctcca tacgtaagta ttataagata ggcatattca cttggttgtc gcagtcgatt
      601 catctcgcta caatcaagga acgcctttat ttggaaagtc caatgtccga gcttcactgt
      661 ccggcctgcg ggggaagcaa aatccggctc gcggccagaa aatctctttc agatgttctc
      721 ctgagcggtc tgacgattta tccgtttcgg tgccagcttt gcgccgatcg ctttcgaaca
      781 ttccttggcc gtcgcacgcc caatccccgt cgtagcttcg agcgtatcga ggtttccttt
      841 ccggtgtggt ttaggccgcg ccggtccaaa tatccagatg agacgggaca agaaggcgta
      901 attgaggatc tatcgatacg tggatgtcgg attcgttcca cgactccggt ggcggtcggc
      961 tcgaggctcg aactggaatt ccagaattct aacgattcct tcccgattac gatcgaagag
     1021 gctgtggtgc ggtcgtcagc caatggtgcg agcggtctgc gttttactcg actcggccga
     1081 aacgatgagc ggcgcattcg ccagctcatc gatgtgtggc tgcccgaact cttgccatca
     1141 agccgagaac gttgatcagc cgggtgctta tggaattcag ggctattc
//
LOCUS       DASTWE010000507         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1241706, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000507 DASTWE010000000
VERSION     DASTWE010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241706"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..358)
                     /locus_tag="VFT30_05285"
     CDS             complement(155..358)
                     /locus_tag="VFT30_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092076.1"
                     /translation="MTQQASAKLLAIIQERRQIRLEELLVSLPEFTWNQVFSLVDELS
                     RRELIFLQRRGFDYELRAASLPI"
     gene            complement(375..878)
                     /locus_tag="VFT30_05290"
     CDS             complement(375..878)
                     /locus_tag="VFT30_05290"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEU5092077.1"
                     /translation="MPWLALESLHLLNDETVRRRLSQLDLLEVWTWGIKGVLSLVILT
                     LLTALTGGALKTFWDIRLLVDHSAEVVLRQIIVNTLILLALVEVFKTTVTYFREGRVK
                     VTFIVDTILVVMLTEVISQWFKGGDWQALAALCGILLVLGVVRVMAVRWSPALRTAAN
                     DIIHNDL"
BASE COUNT          248 a          265 c          256 g          243 t
ORIGIN      
        1 acgttggaat cattgggtcg gatagcggaa acgctggaag cctatcgctg gcttaggcgg
       61 gaggatgcgt cctatcggga tgtcgctgaa cgtattgagc gactgagttc tcgtcgccca
      121 gtggcctcga agaggagcta gctcgagacg gtggctaaat gggcaaagaa gcggctcgca
      181 gttcgtagtc gaaccctcgc cgctgcaaga aaattaattc ccgacgactg agttcgtcta
      241 ccagcgagaa cacctggttc caggtgaatt cgggaagaga taccagaagt tcctcaagtc
      301 tgatctgtcg tcgttcttgg atgatagcca ataatttagc cgatgcctgt tgagtcattg
      361 tcccttctcc ttggtcacag atcattgtga atgatatcat ttgccgctgt cctcaaggcc
      421 gggctccatc gaacggccat gacacgaacg actcctaaaa ccagcaaaat tccgcagagc
      481 gctgccaatg cttgccagtc tcctcctttg aaccattggg aaatcacttc cgtcagcatg
      541 acgactagga tcgtatcgac aatgaacgtc accttaacgc gaccttctcg aaaatatgtc
      601 accgtcgttt tgaatacttc gacaagagcc agcaagatca gcgtattgac gatgatttgc
      661 cgcaacacga cttcagccga gtgatcgacg agcaagcgga tatcccaaaa cgtttttagt
      721 gcgccaccgg ttagggcggt cagaagcgtc aggatcacca ggctgagcac tcccttaatg
      781 ccccaggtcc aaacctccag aagatcgagt tgcgagagcc gccgccggac ggtctcgtca
      841 ttaagtaaat gcaacgattc aagtgctagc cacggcatgc ttcttccctt tctatctggg
      901 tatcaaaata tctagtgact gttcctacat tgtcacctcc atgtaaaact tgtgttaatt
      961 gcaccggttc cctgtggtag gctcgtgtcc cactatatag agaggcggtg gt
//
LOCUS       DASTWE010000508         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_498515, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000508 DASTWE010000000
VERSION     DASTWE010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498515"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFT30_05295"
     CDS             complement(<1..439)
                     /locus_tag="VFT30_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092078.1"
                     /translation="MTGDAWLVETYFDWLRSDCFSEASERREYDGVLRILHDIPFYWT
                     IWSDENRAGDALSYRQNDFLAFQRDLDGLDQRWLHDWAQATPSVLEVLLGIARRWHAY
                     FEGSVPYYFGHMFVNMQFDRMPGRRLSHDTEAHIREKCDDWMSH"
     gene            complement(436..1020)
                     /locus_tag="VFT30_05300"
     CDS             complement(436..1020)
                     /locus_tag="VFT30_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092079.1"
                     /translation="MRFFVGVSAAFGAGTLIGVALGIISTEQKARAKYNESTQSFIRA
                     MELARNNKPAHVEVHVHDESEKAAMRLLEGDPIVSAEPIKQADVTDFKPESNNPYHES
                     LANPTPSWTYLEEEDYHDEDGRYKGQITIMPNESGNPIFVENDIEITDWETKIGSNIL
                     RDFYTLVEPNANPPVLYVRNNLTEEDYEVIKEVP"
     gene            complement(1017..>1561)
                     /locus_tag="VFT30_05305"
     CDS             complement(1017..>1561)
                     /locus_tag="VFT30_05305"
                     /inference="COORDINATES: protein motif:HMM:NF040385.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6353 family protein"
                     /protein_id="HEU5092080.1"
                     /translation="AFTAVTDAFAKYRSRVVAEQGHEADQRFAHGYIIKEVVDGETGE
                     TALITTRDWDDDTDPYNRVFGQGETSQWENDLGLNKMFVSQMQRLCQEKLMRRGYLWL
                     SEVYECLGFPENDICRIVGWRVRTLPDGSRDIPHVDFGLDHPHPDDWKYNRENAIYLD
                     FNVQGMIVGGKVQRALEAAR"
BASE COUNT          316 a          468 c          436 g          341 t
ORIGIN      
        1 gatgcgacat ccagtcatca cacttctccc gaatatgggc ctctgtgtca tgagacaacc
       61 gacgtccggg catgcgatcg aactgcatgt tgacaaacat gtgaccgaag taatacggga
      121 ccgatccctc gaaatatgca tgccagcggc gtgcaatccc aagtagcacc tcaagcacgg
      181 acggcgtagc ctgtgcccaa tcgtgcaacc atcgttgatc gaggccatca aggtctcttt
      241 gaaatgcaag gaagtcattc tgacgatagc tcaaagcatc gccagcacga ttttcgtccg
      301 accagattgt ccagtagaac ggaatatcgt gcaggatccg aaggaccccg tcgtactccc
      361 ttctctcaga agcctcggag aaacagtccg accgcaacca gtcgaaatat gtctccacca
      421 gccaagcgtc acccgtcatg ggacctcttt gatgacctcg tagtcctctt cggtgagatt
      481 gttccggacg tagagcacgg gaggattggc gttcggctcg accaacgtgt agaagtcacg
      541 caggatgttg gaaccgatct tggtctccca gtcggtgatc tcgatgtcgt tctccacgaa
      601 tatggggtta ccagactcgt tcggcatgat ggtgatctgg cccttgtacc ggccatcttc
      661 atcgtggtag tcctcctctt cgaggtaggt ccacgatggc gtcgggtttg ccaaggactc
      721 gtgatacggg ttgttggact ccggcttgaa gtccgtgacg tcggcctgct tgatcggctc
      781 ggcagagacg atgggatcgc cctccagaag cctcattgct gccttctcgc tctcgtcatg
      841 aacgtgaacc tcgacgtgag caggcttgtt gttgcgtgca agctccatcg ctcggatgaa
      901 cgactgagtg gactcgttgt acttcgcacg agccttctgc tcggtggaga tgatccccag
      961 agcaaccccg atgagggtgc cggcaccgaa tgcggccgac acccctacga agaacctcac
     1021 cgcgccgcct cgagagctcg ctggaccttt ccgcccacga tcatgccctg gacgttgaag
     1081 tcgaggtaga tggcattctc gcggttgtac ttccagtcgt ccggatgagg atggtccagc
     1141 ccgaagtcga cgtgcggaat atcccgagat ccgtcgggca acgtgcgcac acgccaaccg
     1201 acgatacgac agatgtcgtt ctccgggaag ccgaggcatt cgtacacctc ggacagccac
     1261 agatatccgc gtctcatcag cttctcctgg cagagtcgct gcatctgcga cacgaacatc
     1321 ttgttgaggc cgaggtcgtt ctcccattgg gaggtctccc cctgcccgaa gacccggttg
     1381 tacgggtctg tgtcgtcatc ccagtctctg gtggtgatca aggcggtctc gcccgtctca
     1441 ccgtccacga cttccttgat gatgtagccg tgggcgaagc gttgatccgc ctcatgcccc
     1501 tgctcggcga cgaccctcga gcggtacttg gcgaacgcat cggtgacggc cgtgaaggcc
     1561 a
//
LOCUS       DASTWE010000509         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_724522, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000509 DASTWE010000000
VERSION     DASTWE010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724522"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="trpD"
                     /locus_tag="VFT30_05310"
     CDS             complement(<1..1007)
                     /gene="trpD"
                     /locus_tag="VFT30_05310"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5092081.1"
                     /translation="MIKEALAKLADRVDLSAQEAETVMLEIMDGAATSAQMAAYLMGL
                     RQKGETVAEVVGSVSAMRSRATRIRVGSSIVVDTCGTGGDGADTFNISTTAAFVAAGA
                     GITVAKHGNRSVSSRSGSADVLSMLGVKIDLEPSRVADCLDEVGIGFLFAPLYHGAMK
                     QCSGVRHEMGIRTILNVLGPLANPAGATHQVLGVYNAKWTDILGRVLLELGSQHCFVV
                     HGLDGLDEITLSDRTKVSEGKGGVVSSYFIAPEEFEVRRTTRKEFVGDSPEENARMTK
                     EILHGGKGPRRDIVCLNAAPAMVVGQKAKTLKDGFRLAQQTIDSGAAAEKLDRLIAFT
                     K"
     gene            complement(1030..>1197)
                     /locus_tag="VFT30_05315"
     CDS             complement(1030..>1197)
                     /locus_tag="VFT30_05315"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HEU5092082.1"
                     /translation="NRVNLPDCCEISAETAEGEIMGLRHRTLCVEGVQFHPESILTTV
                     GKDLLRNFLKL"
BASE COUNT          259 a          368 c          299 g          271 t
ORIGIN      
        1 ttcttagtaa acgcaatgag tcgatccaac ttctctgcgg cggccccgga gtcgattgtt
       61 tgttgtgcaa gacggaatcc gtccttgagt gttttcgctt tttggcctac gaccatcgca
      121 ggtgcggcgt tcagacacac gatatcccgt ctcgggccct tcccgccatg aagaatctct
      181 ttcgtcatcc gtgcgttttc ctcaggtgaa tctccgacga attcttttcg cgtcgtacgc
      241 cggacctcga actcctccgg tgcgataaaa taactcgaca ccacaccgcc cttcccctca
      301 gacactttcg tccggtccga caatgtaatt tcatccagac catccagtcc gtgaaccacg
      361 aaacaatgtt gtgatccgag ttccagaaga acgcgcccaa ggatatctgt ccactttgca
      421 ttatagaccc caagcacttg atgcgtggcg ccggccggat tcgccagtgg gccgagcaca
      481 ttcagaatgg tccgaattcc catttcgtgt cgtaccccgg aacactgttt catcgcgccg
      541 tgatagagcg gtgcaaacaa gaagccgata ccgacttcgt cgagacaatc ggccacccgg
      601 ctcggctcga ggtcgatctt cacacccagc atgctcagca catccgcgct gccggatcgg
      661 gaggacacag aacgattccc gtgcttggcc acggtaatac cggccccggc ggccacgaat
      721 gccgcggtgg tagagatatt aaacgtgtcg gccccatccc cccccgttcc gcaggtatcg
      781 acaacgatcg acgacccgac cctgattcgg gtcgctcgtg atcgcatggc gctgaccgaa
      841 ccgacgactt cagcgactgt ttcacctttt tgcctgagtc ccatgagata ggcagccatc
      901 tgagccgagg tggcagcccc atccatgatc tccagcatga ccgtctcggc ctcttgcgca
      961 gaaaggtcga ctcgatcagc caatttggca agtgcctctt tgatcatggc tacacagagc
     1021 aggagttggc tagagtttca aaaagtttcg gaggagatct tttccgacgg tcgtcagaat
     1081 cgattcggga tggaattgga ccccttccac acaaagtgtt ctatggcgga gccccatgat
     1141 ttcaccctcg gcggtctcag cggaaatctc acaacaatcc ggaagattga ctcggtt
//
LOCUS       DASTWE010000510         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_272105, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000510 DASTWE010000000
VERSION     DASTWE010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272105"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="alaS"
                     /locus_tag="VFT30_05320"
     CDS             complement(<1..1202)
                     /gene="alaS"
                     /locus_tag="VFT30_05320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5092083.1"
                     /translation="MKNSANDLRRAFIRYFEEHGHQAVPSSALIPQADPTLLFTNAGM
                     NQFKRVFLGEESRPYKRAVSVQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDY
                     FKDDAIRFGWEFLTQTVGLQKDRMWVTIFREDDEAEKLWKKIGVLPSRIVRCDEKDNF
                     WQMADTGPCGPCSEIHFDQGPSVPGDDRPNSDGDRVIEIWNLVFMQYNRDASGTLHPL
                     PRPSIDTGMGLERLTAVAQGVYSNYDSDLFTPLLAAIAGRAGTEYGKKESSDRSMRVI
                     ADHLRASTFLMTDGVLPSNEGRGYVLRRILRRAARHGRLLGIVEPFLYELSATVVEQM
                     AVAYSEVRAASGTIAEATKGEEERFIATLDQGLPILSEMIEQAKAAKRTVLAGGDIFK
                     LYDTYGFP"
     gene            complement(1192..1644)
                     /locus_tag="VFT30_05325"
     CDS             complement(1192..1644)
                     /locus_tag="VFT30_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEU5092084.1"
                     /translation="MQLGVRFLARRDRTVVQFERYLLSKGASPLQVRQIVRRLSDLSY
                     LNDYDYAQRWVNKRLVSRPMGQERLRAELQAKGISEVLAARVTAEAFREIDERVLAQR
                     ALQTAQHHGRRLTRSQTIYLLRQRGFSEETIDRMIEESRINEESVYEE"
     gene            complement(1699..>2115)
                     /locus_tag="VFT30_05330"
     CDS             complement(1699..>2115)
                     /locus_tag="VFT30_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEU5092085.1"
                     /translation="YSSVRLDIRRIESIKEGQEVMGSRVRVKVVKNKMAPPFRQAEFD
                     IMFAEGISKTGELVDLGVDKKIIEKSGAWYSYKGERIGQGRDAAREFLKNNGSTAREI
                     ETKLREAAGVPARSEKKAEIKEEKPVSRSEEKRAHR"
BASE COUNT          432 a          642 c          540 g          501 t
ORIGIN      
        1 atggggaagc cgtaagtatc atagagtttg aagatatcgc ctcccgccaa gaccgttcgt
       61 ttcgccgcct tcgcctgctc aatcatctca ctcaaaatcg gtaagccttg gtcgagcgtc
      121 gcgatgaacc gctcctcttc gcctttcgtc gcttcggcga tcgtgccgga tgccgcacgc
      181 acttctgaat aggcgacggc catctgctcc acgactgttg cgctgagttc gtacagaaaa
      241 ggctcgacaa tgccgagtag ccggccatgg cgggccgcgc ggcgcagaat ccgcctcaac
      301 acataccccc gtccctcgtt cgatggcagc acgccatccg tcatcaaaaa cgtactggcg
      361 cgcagatggt ccgcaatcac gcgcatcgaa cggtccgagg attccttctt accgtattcg
      421 gtaccggccc gtccggcgat agccgcaagt agcggcgtga agagatcgct gtcgtaattg
      481 ctgtaaacac cctgggccac ggccgtcaac cgctcgagcc ccatccctgt gtcgatgctc
      541 ggcctcggta gtgggtggag cgtgccagag gcatcgcgat tgtactgcat gaaaacgaga
      601 ttccagatct cgatcactcg gtcgccgtca ctgttcggtc tgtcgtcacc gggtacggac
      661 ggcccctggt cgaagtgaat ttccgagcat gggccacagg gacctgtgtc cgccatctgc
      721 cagaagttgt ccttttcgtc gcaccggacg atgcgcgacg gcaagacacc gattttcttc
      781 cacaacttct cagcttcgtc gtcctcgcgg aagatcgtca cccacatccg gtctttctgc
      841 agcccgaccg tctgcgtcag aaactcccat ccgaatctaa tcgcatcgtc cttgaaataa
      901 tcgccgaatg aaaagttgcc gagcatctcg aagaacgtgt ggtgccgcct ggtatacccc
      961 acgttttcaa gatcgttgtg tttcccgccg gcgcgaagac acttttgcac ggatacggct
     1021 ctcttgtagg gacgggattc ttctccaagg aagacccgct tgaattgatt cataccggcg
     1081 ttggtgaaga gcagggtcgg gtccgcctgt ggaatcaagg ccgagctagg cacggcttga
     1141 tggccgtgtt cctcgaaata acggataaag gcccgtcgca gatcattcgc gctattcttc
     1201 atacacagat tcctcgttga tcctggactc ctctatcata cgatcaatgg tctcctcact
     1261 gaaaccccgc tgacggagaa gatatattgt ttgagagcga gtcaaccgac ggccatggtg
     1321 ttgagcagtc tgaagtgcgc gctgagccaa taccctttca tctatttcac ggaaggcctc
     1381 agctgtcacc cgagcagcca agacctcgga aattcctttg gcctgcaatt cggccctcag
     1441 ccgttcttgt cccattggtc gggagactag ccgtttgttg acccatcgtt gagcatagtc
     1501 gtagtcgttg agatagctga ggtcggacaa acggcgaact atttgccgta cctgaagggg
     1561 tgaggctcct ttggataaga gataccgttc gaattgaacg acggtccgat ctcgacgcgc
     1621 gagaaaccgt actccgagct gcatccaatc cccgggggaa gattggcctc tccgcttcgg
     1681 cgctttcacc gcgtgccgct atcgatgcgc ccgtttttcc tcacttcgtg aaacaggctt
     1741 ttcttctttg atctcagctt ttttctcact gcgtgccgga acgccggccg cctctcgaag
     1801 tttcgtttcg atctcgcgag ccgtagatcc attattcttg agaaactctc gggcggcgtc
     1861 gcgcccctgg ccgattcgtt cccccttgta agaataccat gcacccgatt tttcgataat
     1921 tttcttgtcg acgcctaaat ccaccaattc gccggtcttg gaaatgccct cggcaaacat
     1981 aatgtcgaac tccgcctgac gaaacggcgg cgccatctta ttttttacaa ccttcacacg
     2041 aacacgactc cccatcacct cctggccctc tttgatcgat tcgatgcgac ggatgtccaa
     2101 ccggacggac gaata
//
LOCUS       DASTWE010000511         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_821816, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000511 DASTWE010000000
VERSION     DASTWE010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821816"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..236)
                     /gene="rpmG"
                     /locus_tag="VFT30_05335"
     CDS             complement(75..236)
                     /gene="rpmG"
                     /locus_tag="VFT30_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5092086.1"
                     /translation="MAKGARDNIILQCTDCKERNYVTTKNKKTTPNRLEFKKFCRRCR
                     CHTLHRENK"
     gene            complement(303..>1093)
                     /locus_tag="VFT30_05340"
     CDS             complement(303..>1093)
                     /locus_tag="VFT30_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5092087.1"
                     /translation="VFMNKVDAVDDAELLELVEMEIRELLSSYEFPGDDIPVIQGSAL
                     KALEGDAAWEPKIDELMQAVDDYIPTPARETDKPFLMPVEDIFTIQGRGTVATGRIER
                     GTINVNEPVEIVGIKDTRNSVVTGVEMFKKLLDSGMAGDNVGLLLRGVERKEIERGQV
                     IAKPGSITPHTKFKAEAYVLTKEEGGRHTPFFTGYRPQFYFRTTDVTGVANLPAGVEM
                     VMPGDNIQMEIELIAPIAMEKGLRFAIREGGRTVGAGTVSEVVE"
BASE COUNT          196 a          335 c          279 g          283 t
ORIGIN      
        1 ttcggatttc ggattttgga ttggtcgtgc tgctatcgag ccgatcgaac tccaagatct
       61 caaccaaaat cgaattattt gttttcgcgg tgcagcgtgt ggcagcggca acggcgacag
      121 aacttcttga actcaagccg attcggcgtg gtcttcttgt tctttgtcgt cacgtagttg
      181 cgctccttac agtccgtgca ctgcagaata atattgtctc tcgcgccttt cgccatttct
      241 tacttctccg tacagcgggc tgttagcccg ttgatgcggc gggctaacag cccgccgtac
      301 cattattcaa ctacttctga taccgttccg gcacctacag ttcgtccacc ctctcggata
      361 gcgaaccgga gtcccttctc catggcgatc ggggcgataa gctcgatctc catctggatg
      421 ttgtctcccg gcataaccat ctcgactccc gccggaaggt tcgcgacacc ggtcacgtcc
      481 gttgttctga agtagaactg cggacggtat cccgtaaaga acggggtgtg gcgtccgcct
      541 tcttctttgg tcaggacata cgcctcggcc ttgaacttcg tgtgcggcgt gatcgaaccc
      601 ggcttggcga taacctggcc tcgctcgatc tcttttcttt caactccgcg aagcagcagc
      661 ccgacgttgt ctccggccat tcccgagtca agcagcttct tgaacatctc gacgcccgtt
      721 accaccgagt tgcgagtgtc cttgatgccg acgatctcga ccggctcgtt gacgttgatc
      781 gttccacgct cgatccttcc cgtcgcaacg gttccacgac cctggatcgt gaagatgtct
      841 tcgaccggca tcaggaacgg cttgtccgtc tcgcgtgcag gtgtcgggat gtagtcatcc
      901 actgcctgca tcagctcgtc gatcttcggc tcccatgccg catcgccttc aagagctttc
      961 aatgccgaac cctggatgac cggaatgtcg tctcccggga actcgtaaga gctgagcagt
     1021 tcgcggatct ccatctccac gagctcgagg agttcggcgt cgtcgacagc gtccactttg
     1081 ttcatgaaca cta
//
LOCUS       DASTWE010000512         1266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1500478, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000512 DASTWE010000000
VERSION     DASTWE010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500478"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFT30_05345"
     CDS             complement(<1..333)
                     /locus_tag="VFT30_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5092088.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKEDIHVIGEAQDGMEAVEK
                     ARALLPDVILMDVSMPRMGGVEATRQVKREFPHIGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESS"
     gene            complement(320..>1266)
                     /locus_tag="VFT30_05350"
     CDS             complement(320..>1266)
                     /locus_tag="VFT30_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5092089.1"
                     /translation="KAPGVGLAKQLNIPITQDYRQLLAMERVDLVIDVSGDGEVWQFL
                     QDFHRMGVTIIGGASAKFMWELIEARIRATAEIEKTLNKYQSLYRLYVKETGAAVTEE
                     RTRIACEIHDGFVQSLAGVNFKLDLCQQLIRKKSRASLATIKETKAQLKLAIQEARQV
                     IFNLRPLHYDKMELIPALTNYFKSYQTQTHIATKFSVTGDEQILFPRTKIFLFRIIQE
                     ALSNVEKHAKADRVSIKLDIDMDLLRVIISDNGIGFDLETVLRDPEKWDHFGIKGILE
                     RARLVGGEGHVQSKPGKGTKIIVEVPLGHKEERGNGEN"
BASE COUNT          266 a          366 c          304 g          330 t
ORIGIN      
        1 agacgattca gagtctttca agagataccc tgtcgcgccc gcgcggacaa gatcgaagat
       61 gtactgttgt tcttcataca tggtcaacgc gacgatgccg atgtgtggaa actcgcgctt
      121 gacttgtctc gtcgcctcga cgccacccat ccgcggcatg ctcacatcca tgagaatcac
      181 atcgggaagc agcgcgcggg ctttctccac tgcttccatc ccgtcttgag cctcgccgat
      241 gacgtgaatg tcttccttgg tcttcagaat ggcggccaag ccttcccgaa ccacccgatg
      301 gtcgtcggcg atcagaacct taattttctc cattccctct ctcctcctta tgacccaacg
      361 gtacctcgac gatgattttc gtacctttcc caggtttgga ttggacgtgc ccctcgccgc
      421 ccaccaagcg agctcgttcc aagataccct tgatgccgaa atgatcccac ttttcagggt
      481 cacgcagaac ggtttccaga tcgaagccga ttccattgtc ggaaataatc acgcgcaaca
      541 gatccatatc gatgtccagc ttgattgaga ctcggtcggc tttggcatgc ttctcgacgt
      601 tgctcaaggc ttcctgaatg attcgaaaaa gaaagatctt cgtccgtgga aagagaatct
      661 gttcatcccc cgtgaccgaa aatttcgtgg caatgtgggt ttgggtttgg tacgacttga
      721 aataattcgt cagggcggga atcaattcca tcttgtcgta atgcaaaggg cgcaggttga
      781 aaatcacctg cctggcttcc tggatggcca gtttcagttg tgccttggtc tccttgatgg
      841 tggccagact ggctcgtgac ttctttcgaa tgagttgctg gcaaagatcc agcttgaagt
      901 tcacgcccgc caagctctgc acgaatccgt cgtgaatctc gcaagcgatc ctcgtccgct
      961 cttccgtgac tgctgctcca gtctctttca catacaatct ataaagggat tgatacttat
     1021 tcagcgtctt ctcgatctcc gccgtcgcgc gaatgcgggc ttcgatgagt tcccacatga
     1081 attttgcgct ggcaccgcct atgatggtga cacccatccg atgaaaatcc tggaggaatt
     1141 gccagacctc tccatcgccc gatacgtcga tgaccaggtc gacacgctcc atcgccaata
     1201 gctgtcgata atcctgtgtg atcgggatgt tgagctgttt ggccaatccc actcccggcg
     1261 ctttct
//
LOCUS       DASTWE010000513         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1177120, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000513 DASTWE010000000
VERSION     DASTWE010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177120"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1114
                     /locus_tag="VFT30_05355"
     CDS             200..1114
                     /locus_tag="VFT30_05355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5092090.1"
                     /translation="MAAASHPVRFGIQTPQQNGSWPEMVALWQELDTLGFDSAWVFDH
                     FLPIFSDPTGPCLEGWTALSALAMVTHRVRIGVMVTGNTYRHPAVLAKMATTLDIVSN
                     GRLNLGLGAGWFALEHQTFGLPFPAVGERLQRLDEALTIIRRLWNEDRVTFTGRHYQL
                     HDALLNPRPIQQPHPPVVVGANGEQVALRIVAQHANMWNAFGSPDVFRHKIAILTEHC
                     RKIGRDPGTIEKSVLIQMMLTDDAEVKRRTLEQESWGMLVGTSSDIRQQIAAYVAAGV
                     THIIISLAAPYDMTTLRRFVTEVIPAFR"
     gene            complement(1166..1304)
                     /locus_tag="VFT30_05360"
                     /pseudo
     CDS             complement(1166..1304)
                     /locus_tag="VFT30_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020875.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          304 a          392 c          397 g          332 t
ORIGIN      
        1 ctctccttgt gcggttcttc ttcttttaat cactatccga cgatgcaaca atatggacga
       61 agaaagtgga tgcgttgtgt gagtcccttt cccaatcctg ctttatcgct tactgacata
      121 cctgtcacta ctgtctagtg cctggtatga catcgtcgta agcccacgtc gtgtagcaac
      181 tgtaagaacg gaggacgcaa tggcagcagc atcccatccc gtacgatttg gtattcagac
      241 cccacaacaa aatggctcgt ggccagaaat ggtcgcctta tggcaagagc tcgatacgct
      301 agggtttgat agtgcgtggg tatttgacca cttcctgccg atcttctccg atcccaccgg
      361 gccgtgtttg gaagggtgga ccgcgttgtc ggcgctcgcg atggtcacgc accgcgtacg
      421 cattggtgtg atggtcaccg gcaataccta tcgccatccg gcggtattgg ccaagatggc
      481 cacgacttta gacattgtga gtaacggacg gttgaacttg ggattaggcg cgggctggtt
      541 tgcgttggag caccagactt ttggattgcc cttccctgcg gtcggcgagc gcttacagcg
      601 cctcgacgaa gccctcacga taatcagacg cttatggaat gaagaccgcg ttacctttac
      661 cggacgacat tatcaacttc acgatgcgtt actgaaccca cgcccgatcc agcagcccca
      721 tccaccggtg gtcgtgggag cgaacgggga gcaagtcgct ttgcgcattg tggcgcagca
      781 cgcgaatatg tggaatgcat tcggctctcc ggacgtcttc cgtcataaga ttgctatcct
      841 cacagagcat tgtcggaaga ttggccgaga ccccggcacg attgagaagt cagtattaat
      901 ccagatgatg ctaaccgacg atgccgaggt caagcgtcgc acgctggagc aggagagttg
      961 ggggatgctt gttggcacat caagtgacat tcggcaacag atcgcggcat atgtcgcggc
     1021 cggtgtcact catattatta tctcgctagc cgctccctac gatatgacaa cactgcgacg
     1081 cttcgtgacc gaagtgatac ccgcatttcg ctgagcctcc gttagggatt cgatgcccat
     1141 aatagtcacg ccacaatcgg acggtgtgga tttgctccca catcccagca ttgcgccacc
     1201 gtcggaagtc gtcggacacc gtctgccagt cgggaaattc tcgcggcagt aagcaccacg
     1261 cttctactct acagcagata gaggatagca ttcagcactt cgcgcatatc gggcgtccgc
     1321 ggtcggagtg tgtgtcggga tggtaggtac ttcgaagtgc tttgagattg gtccgcacgc
     1381 cacgtgaacg cttgcccacg ggcttacgcc gcgggggtac acgcg
//
LOCUS       DASTWE010000514         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1597594, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000514 DASTWE010000000
VERSION     DASTWE010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597594"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFT30_05365"
     CDS             <1..194
                     /locus_tag="VFT30_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092091.1"
                     /translation="KCAGIHNILSFLAPKLLITIQEKKRFVASLSRGGSFLLDKEGKA
                     IREFATSIHIRDAVTGRAI"
     gene            194..>1116
                     /locus_tag="VFT30_05370"
     CDS             194..>1116
                     /locus_tag="VFT30_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5092092.1"
                     /translation="MFNHPVISADGHIDLPWLPPTLFVDQAPAALKDKMPKVVDAPEG
                     KTWVAHNGTNLGLAGGMGSAGRPYIPGQIYRSDRMASEGLYEDYKRGIFRTSDPKYRL
                     LDQDRDGVVGEVLYGILGASFRLQDPEIVETVVRIYNDFAADFAKACPERLATIACLP
                     TFSPGKAGEELRRCAKIGLKGAEIPISPGMMPLWHEDWNPLWEAAHDCHIPIHFHTIG
                     PKQDMSWLTNHRARRLWLATLLAGFQMSMVDAMAAIIYSGALERLPQLKIVIGEAGIG
                     WIPYVLERLDYEWEDQFKDLDLKMKPSEYWYR"
BASE COUNT          247 a          297 c          317 g          255 t
ORIGIN      
        1 taaaatgcgc gggaattcac aatatcttgt cgtttctagc tccgaaactc ctcattacca
       61 tacaagagaa aaagagattt gtcgcttcgc tttccagagg cgggtcattt ctgctagata
      121 aagagggcaa agcgattcgc gaatttgcga caagcatcca catacgcgac gctgtcacag
      181 ggagggcgat ctgatgttta atcatccggt gatttccgcc gacggacata tcgatttacc
      241 gtggctgccg ccgacacttt ttgttgatca ggcaccggcc gcgctgaaag acaaaatgcc
      301 aaaggtcgta gatgctcctg aaggcaaaac gtgggtcgcc cacaacggca ccaacctcgg
      361 actcgcggga ggaatggggt cagccgggcg gccgtatatt cctgggcaga tttatcgttc
      421 ggatcgtatg gcgtcggaag gactgtatga ggactacaag cgtggcatct ttcgcacgtc
      481 tgatccgaaa tatcgtctgc ttgatcagga ccgcgatggc gttgtcgggg aggtcctcta
      541 cggtattctt ggcgcttctt tccggttgca agaccccgaa atcgttgaga cggttgtgcg
      601 tatctacaac gactttgccg cggattttgc gaaagcctgt cccgagcgtc tggcaacgat
      661 cgcttgtctt cccacctttt cccccgggaa agccggtgag gagctgcggc gctgcgccaa
      721 gatcgggctc aaaggcgcag agattcccat ttctccgggc atgatgccgc tgtggcacga
      781 agactggaat ccgctgtggg aagccgcgca tgactgtcat atccctattc actttcacac
      841 gatcgggcca aagcaagata tgagttggct caccaatcat cgcgctcgcc gattgtggct
      901 ggcgacgctc ctcgcggggt ttcagatgtc gatggttgac gccatggccg cgatcatcta
      961 cagcggcgcg ctcgaacgtt taccgcagct caagatagtc atcggtgagg ccggtattgg
     1021 ttggattccg tacgtcctcg aacggctcga ctacgaatgg gaagaccagt tcaaggatct
     1081 cgacctgaag atgaagccca gcgaatactg gtaccg
//
LOCUS       DASTWE010000515         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_854081, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000515 DASTWE010000000
VERSION     DASTWE010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854081"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFT30_05375"
     CDS             complement(<1..323)
                     /locus_tag="VFT30_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092093.1"
                     /translation="MLSNTAVPNHYAAFREKVLRGEIPVCEEVSLQMQRIDQLVADRN
                     VYYDPEAIDGFIEFCEEEMTLTDGADVHMLDSFKLWAEDLLSWFIFVERERWDEESGQ
                     FVMKV"
     gene            complement(327..659)
                     /locus_tag="VFT30_05380"
     CDS             complement(327..659)
                     /locus_tag="VFT30_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092094.1"
                     /translation="MSSILQDVKHMLGIPQSNTVFDSDVLVYINTAFGDLTQLGVGPE
                     IGFQITGPDNTWEQFYTDPRLNAVKSYVYLKAKLLHNPPEMGFLVTAMENQLQEMAFR
                     LNVVADYG"
     gene            complement(678..>1094)
                     /locus_tag="VFT30_05385"
     CDS             complement(678..>1094)
                     /locus_tag="VFT30_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092095.1"
                     /translation="TELGVILSVVAIAAAIIRDLYKTFVVDRRQDKQNIESAKAQQPE
                     IMKQLEAGNFKAAAEGIAIAQTFVRDQLTYAQAEIKRLRERETALEAEAAGWEKRDEE
                     RDARMLELEKRVTRAEARAELLQEQLAECKRNILGK"
BASE COUNT          220 a          316 c          281 g          277 t
ORIGIN      
        1 atgactttca tgacgaattg acctgattct tcgtcccatc gctctcgctc gacgaatatg
       61 aaccagctca agaggtcctc ggcccacagc ttgaacgaat caagcatatg gacgtcggcc
      121 ccgtctgtga gggtcatctc ctcttcacag aactcgataa acccgtcgat ggcttccgga
      181 tcgtagtaca cgttccggtc tgcgaccagc tgatcgatcc tctgcatctg aagagagact
      241 tcctcacaga cggggatttc gcctcgaagc accttctctc gaaaggctgc gtagtgattc
      301 ggaacggcgg tgttactaag catggatcac ccgtagtcgg cgacgacatt cagtcggaac
      361 gccatctcct gcagctgatt ctccatggcc gtgaccaaga aacccatttc gggagggttg
      421 tgcaagagct tagccttgag gtagacgtag ctcttcacag cgttgagacg cgggtcagtg
      481 tagaactgct cccacgtatt gtcaggtcca gtgatctgga acccgatctc gggaccaacg
      541 ccgagttgcg tcaggtcacc gaatgccgtg ttgatgtata ccaacacgtc gctgtcgaac
      601 actgtgttcg actgtggaat gccgagcatg tgcttgacat cctgcaggat gctgctcacg
      661 tcaaccctcc cttctggtta cttgcccaat atgttccgtt tacattctgc gagctgttct
      721 tgcagtagtt ccgctcgggc ctctgcacgg gtgactcgct tctctagctc aagcattcga
      781 gcatcgcgtt cctcgtcgcg tttctcccaa cccgcagcct ctgcttcgag agcagtttct
      841 cgctcacgaa gacgtttgat ctcggcttgg gcgtatgtca gttggtctct gacgaaggtt
      901 tgcgcgatag caataccctc cgccgcggcc ttgaagttac cggcctccaa ctgcttcatg
      961 atctcgggct gttgggcctt cgctgactca atgttctgct tgtcttgacg gcgatcgact
     1021 acgaaagtct tgtacagatc tcgaatgatc gcagcagcga tggcgacgac cgacaatatg
     1081 acgcctagtt cagt
//
LOCUS       DASTWE010000516         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_531198, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000516 DASTWE010000000
VERSION     DASTWE010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531198"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFT30_05390"
     CDS             <1..316
                     /locus_tag="VFT30_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5092096.1"
                     /translation="RVSLADMLKAASGTGQLSENPHAVNNAVDAYAIMGVEFGSSLAD
                     VTAAFRIKAKQLHPDANNGDRSSEPELRRMLEAYQFLKEYLSLSNTEPMPPPDRPYSP
                     SE"
     gene            330..>1273
                     /gene="rnd"
                     /locus_tag="VFT30_05395"
     CDS             330..>1273
                     /gene="rnd"
                     /locus_tag="VFT30_05395"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU5092097.1"
                     /translation="MPIPQYITSPTALSELCERLRDSPRLALDTEFVGEESFVPKLEL
                     IQVATERTAAIIDFPAVQSGGALDGFWEIVCDSRIQKVVHAGRQDLDLFAVHAGRIPK
                     PFFDTQIAAAMVGFGPQVAYANLVQRVHGKRLDKAHTFTNWSARPLSQDQLAYALEDV
                     TFLLAIHDHLHTRLSSLGRLQWADEEFSRLEGVVGEARREPQERYQRIRGWDQLKPKS
                     AAVLRELAAWREGEAKRRNVPRSRVVRDEVLLQLARQPPRHQDELRKVRGLHASEVDR
                     NGESILGVIQAALALPASAWPALAKERKPEPESNGLVEL"
BASE COUNT          285 a          352 c          376 g          260 t
ORIGIN      
        1 acgagtcagt ctcgccgata tgctcaaagc cgccagtggg accggacagc tgtcagaaaa
       61 cccccatgcc gtgaataacg cggtggatgc ctatgcgatt atgggggtgg agttcggcag
      121 ttctctcgct gatgtcaccg cagcgttccg aatcaaggcg aagcagctcc atcccgacgc
      181 gaacaacggt gatcgcagtt ctgaaccgga actccgccgc atgttggaag cgtatcagtt
      241 tctgaaggaa tacctcagtc tcagcaatac ggaacccatg cctccacctg atcgccccta
      301 cagcccgtct gagtgacgca ccagtgacga tgcccatccc tcagtacatc acgagcccaa
      361 ccgcactcag cgaattgtgc gagcgattgc gggacagccc gcgcctggcg ctcgatacgg
      421 aattcgtggg cgaggagagt tttgtaccga agctcgaact cattcaagtc gcgaccgaac
      481 gcactgcggc cattatcgat tttccggccg ttcagtcagg gggagcgctc gatggattct
      541 gggagattgt atgcgattcg aggattcaga aggttgtgca tgcaggacgt caagatctgg
      601 acctcttcgc agtccacgcc ggtcgaatcc caaaaccatt cttcgacacc cagattgcag
      661 cagcaatggt cggcttcggc cctcaggttg cctatgccaa tcttgttcaa cgggtgcatg
      721 gaaagagact ggacaaagcg cataccttta cgaattggag cgcccgcccg ctgtcccaag
      781 atcagttggc ttatgcgctg gaagacgtga catttctatt ggcgattcac gatcatctcc
      841 acacccggtt gtcgagcctg gggagattgc aatgggcgga cgaagagttt tctcgccttg
      901 aaggtgtcgt gggcgaagct cgtcgagaac cgcaggaacg ctatcaacgt atacgaggat
      961 gggatcagct aaagccgaaa tcggccgcgg tgcttcggga acttgccgct tggcgtgagg
     1021 gggaagcgaa acgcagaaat gtgcctcgga gtcgggtcgt ccgtgatgaa gtgctgcttc
     1081 agctggcgcg acagcctcca cgacaccagg atgagttgcg caaagtgcga ggcctccatg
     1141 cttcagaagt tgatcgcaat ggtgagtcga tccttggtgt gattcaagcg gcacttgcgc
     1201 tcccggcatc ggcttggccg gcgctcgcca aagagcgaaa gcctgagccg gaatcgaatg
     1261 gactcgtaga gct
//
LOCUS       DASTWE010000517         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1404057, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000517 DASTWE010000000
VERSION     DASTWE010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404057"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFT30_05400"
     CDS             <1..591
                     /locus_tag="VFT30_05400"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEU5092098.1"
                     /translation="IELVSDVVEQGIGMIGTGEMGIGNTTISSAITAVMTGRPVEEVT
                     GRGTGVDDAVYRHKISVIKEALAFHRPDPSDALEVLSKVGGLEIAGLVGLILGSALAR
                     VPVVLDGFITGAAALIAARLQPKCLGYMIASHRSVERGHQLLLDHLGIKPLLDLDLRL
                     GEGTGACLAMNLMCAAVKVMTEMATFDEAGVSERNA"
     gene            588..1352
                     /locus_tag="VFT30_05405"
     CDS             588..1352
                     /locus_tag="VFT30_05405"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEU5092099.1"
                     /translation="MTTLVRPFLFAWHFLTAIPLSRHHHEPTAMELASSMAWYSTVGL
                     LIGGTLALADTVLTKLFALEVVNVLLIAFLIVLTRGLHQDGLADTLDGIAGGRTPADR
                     LSIMRDPRIGAIGATGLFMSLILRYAALMALPHPLRVSALVCMPALGRWAMVTLAWLS
                     PYARTEGGLAAPFLVHLSLLHVLGSTFVLASALVLMLGVSGAVAILVGGSAVVCAVQA
                     GCRPLFGGITGDTLGATNEAVEILFLLSVPLLLVLS"
BASE COUNT          246 a          349 c          412 g          347 t
ORIGIN      
        1 attgaacttg tgtccgatgt ggtcgaacag ggaatcggaa tgattggtac cggtgaaatg
       61 ggaatcggga acacgacgat cagctctgcg atcacggccg tgatgacagg tcggccagta
      121 gaagaggtga ccgggcgcgg aacaggagtt gacgatgccg tctatcgaca caagatatcg
      181 gtgatcaagg aagcgttggc gttccaccgg ccggatccga gtgatgcatt ggaagtcctt
      241 tcaaaagtcg gtggactcga aatcgcagga ctcgttggcc tcattcttgg gtcggctttg
      301 gcaagagtcc cggttgtact ggatggattc atcaccggag ctgccgcatt gatcgccgct
      361 cgactgcagc ccaaatgtct tgggtatatg attgcctccc atcggtctgt cgagcgaggc
      421 catcagctgt tgctcgatca tcttggcata aaaccgttgc tggatctcga cctacggctc
      481 ggtgagggaa cgggggcgtg tttagcgatg aatctgatgt gtgccgccgt caaagtcatg
      541 actgaaatgg caacattcga cgaagccggc gtgtcggagc gcaacgcatg acgaccctgg
      601 ttcggccatt tcttttcgcg tggcattttt taaccgcgat ccccctcagt cgacatcatc
      661 atgaacccac cgcgatggag ttggcctcat cgatggcctg gtattccacc gtaggccttc
      721 tgatcggcgg gacattggca ctcgcggaca ctgtgctgac gaagttgttt gcgttggagg
      781 tcgtgaatgt attgctgatc gccttcctta ttgttttaac tcgcgggtta catcaggacg
      841 gcttggccga cacgcttgac ggtattgcag gaggcagaac accggctgat cgcctgtcca
      901 tcatgcgtga tccgaggatc ggtgcgatcg gagcgaccgg actttttatg tcgttgatcc
      961 tccgctatgc ggcgctcatg gccctcccac atcctctccg tgtctcggct cttgtctgca
     1021 tgccggcgct cggccgttgg gcaatggtga cattggcatg gctgtctccc tacgcgagaa
     1081 cagagggtgg gttggcagca ccgtttcttg ttcatttgtc attgctccat gtgctgggat
     1141 cgacgttcgt acttgcctca gcactcgttc tgatgctggg tgtctccggg gcagtcgcaa
     1201 tattggtcgg cggtagtgcc gtggtttgtg cagttcaggc aggttgtcgc cccttgttcg
     1261 gtgggattac gggagatacg ctgggcgcca cgaatgaagc cgtcgagatc cttttcttgc
     1321 tctccgtccc gttgttgcta gttctgtcat gacg
//
LOCUS       DASTWE010000518         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1274364, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000518 DASTWE010000000
VERSION     DASTWE010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274364"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..522)
                     /locus_tag="VFT30_05410"
     CDS             complement(268..522)
                     /locus_tag="VFT30_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092100.1"
                     /translation="MVSKQELQVQQKRQVEDKEEATIPVRQFLPNTDIYETETALTVV
                     LEMPGVEKTNVEVAVEGGVLKVYGRIDFSKYEGLQPIYTE"
     gene            complement(537..965)
                     /locus_tag="VFT30_05415"
     CDS             complement(537..965)
                     /locus_tag="VFT30_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5092101.1"
                     /translation="MSMMYSDPFAALLGLQRALEARQASDWLREATTGTGSFPPINVF
                     QQGEGGFVAIIELPGVSKRDLNIQAKENTIRIAGKKSIQYEEGASVHRRERVSGEFDR
                     TLTVPVEIDPDGIKAQYRDGVLALFIPIGEKAKPRSIEIS"
BASE COUNT          271 a          419 c          397 g          338 t
ORIGIN      
        1 gattcaccgg cgaggatagg acgggggaca gcattgagcg aactcggctg tccccggctt
       61 ggcgttccac gttagttccc gatatattcg agagcacccg ctcatttaga cggcgatggg
      121 ggatcacgtc actgaaatcg cgcgcggctt cgcttcctct accttaggaa gaacaagcga
      181 gaggacgcca tcattcatct gcgcttcgat tttggtctgg tcgatcttgc tcgacaaacg
      241 aaagcttctc gcatagtgac cgatgttcta ctccgtgtag atgggttgca gaccttcgta
      301 tttggaaaag tcgattcgcc catagacttt gagaactccg ccttcaactg ccacctcgac
      361 attggttttt tcgactcccg gcatctccag aacgacggtt agggcggtct cagtttcgta
      421 gatgtcggtg ttcggtagaa attgtcggac tgggattgtt gcctcttcct tgtcttcgac
      481 ctgccgcttc tgctggacct gcagttcttg cttagaaacc atggcgccct cccgtcctag
      541 ctgatctcga ttgaccgagg tttggctttt tcgcctatcg gaatgaataa cgccaagacc
      601 ccatcgcgat attgcgcctt gatgccatca ggatcgattt cgaccggcac cgtcagggtg
      661 cggtcgaact cccccgaaac acgctcgcgg cgatgaacac tggcaccctc ttcgtattgg
      721 atcgatttct tccccgcgat gcggatggtg ttctccttcg cctggatgtt gaggtccctt
      781 ttgctgactc ctggaagttc aatgatggcg acaaagccgc cctcgccctg ttggaagacg
      841 ttgatgggcg ggaaggaacc cgtcccggtc gtcgcctccc gaagccaatc gcttgcctga
      901 cgggcttcga gagcacgttg cagtcccaac agtgcggcga aggggtcgct gtacatcatg
      961 ctcatgactc gctccctagc aatcgcacct gttcgcttcg ggtcggacgt tcagccgacc
     1021 cgcttttgat ttggtacgcg cgttagcact gtcaagtcca gatcggtggg cagtcagcgc
     1081 acgggcggcg cggcctagct tatccgctcc gcaatcgccg ttgcccccgg cgtcagccgt
     1141 ttcctcttct tgggcatacg agtctcctcg acaatagatg ctgtcaatac aaaacgggtc
     1201 gcaccggcga gaaaaacgta cgatcaaacg taagggttcc ctccccgact gcggaaccgg
     1261 catcggccga accgcgttgg tgcgcattcg gagcttctca tgaaagcgcg gtatttccat
     1321 caccagcccc gatgtggagg cctcggcccc atcgcccacg cggttgagga ggatgatcag
     1381 attgagacct ggatgatcgg tcagggtccg gagctgagcg gcaac
//
LOCUS       DASTWE010000519         1220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_627960, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000519 DASTWE010000000
VERSION     DASTWE010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627960"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFT30_05420"
     CDS             complement(<1..352)
                     /locus_tag="VFT30_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5092102.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLKAVAEKYGHSFEFVAADIG
                     GQAIDKVGVPLPKDTLTLAKQSDAVLLGSVGGPRWEGLEYSLRPERALLGIREALGLY
                     ANLRPAKLYSNLVDA"
     gene            complement(386..748)
                     /locus_tag="VFT30_05425"
     CDS             complement(386..748)
                     /locus_tag="VFT30_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5092103.1"
                     /translation="MFKVTLADRPEFLAGDETRLREIFHPVKHQLKLNYSLAHGTLPS
                     GQRSKRHVLASSEVYYFISGRGCFTIDDHVTPIEAGTTIYVPPGGQQFLENTGETDVE
                     FLCLVDPAWRLEDETILE"
     gene            complement(813..>1220)
                     /locus_tag="VFT30_05430"
     CDS             complement(813..>1220)
                     /locus_tag="VFT30_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEU5092104.1"
                     /translation="EELSKMAERIGLKALQVSSGTDRVPTATVELEIDGTPIVQTGTG
                     DGPVDAVYRTIAAMTQTKSKLLMYVVKAITGGTDAQGEVSVRVQEDGRTVTGHGADTD
                     IITASARAYISALNKLAYLATKQAQGEQKVNLI"
BASE COUNT          253 a          387 c          309 g          271 t
ORIGIN      
        1 aagcatccac cagattcgaa tagagcttgg ccggcctcaa gttggcatag aggcctaagg
       61 cttcccggat tccgagcagt gcgcgctccg gccgcaggct gtattccaga ccttcccatc
      121 gaggccctcc gacggagccc aacaagacgg catcgctttg cttggcaagc gtcaatgtgt
      181 ctttcggcag cggcacaccc actttatcga tcgcctgccc gccgatatcg gctgcgacaa
      241 actcgaatga gtgcccgtac ttctcggcaa cggccttcag gaccttcacg gcttcgggaa
      301 cgatctcgcg cccgactccg tcaccggcaa gcactgcaat cttggccttc actgctgctc
      361 ctttcgatga cgcaatcggc tctgcctatt ccagtatcgt ttcatcttca agccgccagg
      421 ccggatcaac gagacacaag aactcgacat cggtttcccc tgtattttcg agaaactgtt
      481 gtcctcccgg cggcacatag atggtggttc cggcttcgat cggcgtgacg tggtcgtcga
      541 tcgtgaagca gccccgccct gaaatgaagt aatagacttc cgacgaggcc aagacatggc
      601 gcttagagcg ttgaccggac ggtaatgtcc cgtgggcaag actataattc agtttgagct
      661 gatgcttgac aggatgaaag atctcccgca agcgagtttc atccccggcc agaaattcgg
      721 ggcgatccgc cagcgtcacc ttgaacacgg acaacccctc tcaacccaac agacctcacc
      781 acggaaatga aactgtaccg tggagatccg cgtcaaatca agttcacctt ctgctcgcct
      841 tgcgcctgtt tcgtcgccag ataagccagt ttattcaagg cactaatgta tgcccgagca
      901 gacgccgtga tgatatcggt atcggcgccg tggcccgtga ccgtccggcc atcctcttgt
      961 acgcgcaccg acacttcacc ctgtgcatct gtcccaccgg tgatggcctt cacgacatac
     1021 atcagcaact tgctcttcgt ctgtgtcatg gcagcaatgg tgcggtatac ggcatccacc
     1081 ggcccatcgc cggtcccggt ttgaacgata ggtgtgccat cgatttccaa ttcgaccgtg
     1141 gctgttggaa cccgatccgt tccgctcgag acctgaagtg cctttaggcc gatgcgctcg
     1201 gccatcttcg acaactcttc
//
LOCUS       DASTWE010000520         1430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1500746, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000520 DASTWE010000000
VERSION     DASTWE010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500746"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1430)
                     /locus_tag="VFT30_05435"
     CDS             complement(<1..>1430)
                     /locus_tag="VFT30_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092105.1"
                     /translation="AVVLPEVNFSWTFCPILTHGIVRGYATDMTSVHKTQQALQFSAD
                     QLRQSNRRLDQALDEANESARVKTAFLATISHELRTPMNGVIGMTSLLLETSLTSEQQ
                     SYAETIRQCGEALLQLINDVLECSKIEAGKLELECLDFNLRTSVEQVLAQFAERAETK
                     GLELTGLVHASVPTALKGDPGRLRQILTNLVANAVKFTGKGEVTLQAYLEEDLPGTAV
                     VRFEVTDTGIGINPNTQAKLFRPFVQADSSTTRKYGGTGLGLSISKQLVELMGGRIGV
                     RSIEGQGSTFWCTARFQKQADSPRAILPTGDLTGKRVLIVDDNESNRLILHNLVSGWG
                     MVDDLAEDAESALRRIAEAKQRGKPYDLAILDVIMPGKDGLQLARELQNHPAGSGIRL
                     VVMTSMLQRGHAEQARQAGAMGYLPKPIRHDELRDCLRTVLGLPEGQAPKDTQTRSMV
                     PQLVTRHMVAENAQYLRILVVEDNIV"
BASE COUNT          266 a          403 c          425 g          336 t
ORIGIN      
        1 tgacgatgtt gtcttccacg accaaaatac gcagatactg cgcattctcc gcgaccatgt
       61 gcctggtgac gagttggggc accatggagc gtgtttgggt gtctttgggg gcttggccct
      121 cgggcaggcc gagcacggtt cgtaggcagt cgcgtaattc gtcatggcga atgggtttcg
      181 gtaggtagcc catcgcaccg gcctggcggg cttgttccgc atggccccgc tgcagcatcg
      241 aggtcatcac gacgaggcga atcccggatc ctgctgggtg attttgtagt tctcgtgcga
      301 gctgcaaccc gtcctttccc ggcatgatga cgtccaaaat cgcgagatcg tagggctttc
      361 cccgctgctt tgcctccgcg atgcggcgta aagccgattc ggcatcctct gcaagatcgt
      421 caaccatccc ccaccctgat acgagattgt ggaggatcaa acgattcgat tcgttgtcat
      481 cgacgatcaa gacacgcttt ccggtgagat cgccggtcgg aagaatcgcg cggggagaat
      541 ccgcctgttt ctggaagcgc gcggtgcacc agaaggtgct gccctgcccc tcgatgctgc
      601 gtactccgat ccgtccaccc atcaattcca cgagttgctt ggaaattgaa aggccaaggc
      661 cggtcccccc gtattttctc gttgtggaac tgtccgcctg cacaaacgga cggaacagct
      721 tggcttgagt attcggattg atgccgatgc cggtgtccgt gacctcgaaa cggacgactg
      781 cggtaccggg taggtcttct tcgagatagg cctggagggt cacctcgccc ttgccggtaa
      841 acttcaccgc gttggcaacc agattggtga ggatctgacg caggcggcct gggtcgcctt
      901 tgagtgctgt cgggaccgac gcgtgtacaa gtccggtcag ttccagccct ttcgtctccg
      961 ctcgttcggc gaattgtgcc aacacctgct ccaccgaggt tcttagattg aagtcgaggc
     1021 actccagttc cagcttgcct gcctcaatct tgctgcattc aagtacatcg ttgatcagct
     1081 gcagcagcgc ttcgccgcat tgccggatag tctccgcgta ggactgttgt tcagacgtca
     1141 aggaggtctc cagcaacagg ctcgtcatac cgattacgcc gttcatgggg gtacgtagtt
     1201 catggctgat ggtcgctaga aatgccgtct tgacgcgtgc cgattcattg gcttcgtcca
     1261 gagcttggtc caggcgacga ttgctctggc gaagttggtc ggcggagaat tggagcgcct
     1321 gctgcgtctt atggacgctt gtcatgtcgg tggcatagcc acgtacgatg ccatgagtaa
     1381 ggatcgggca gaaggtccat gaaaaattga cctctgggag cacaactgcc
//
LOCUS       DASTWE010000521         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_78771, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000521 DASTWE010000000
VERSION     DASTWE010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78771"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..818)
                     /locus_tag="VFT30_05440"
     CDS             complement(189..818)
                     /locus_tag="VFT30_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU5092106.1"
                     /translation="MLHYIVPDSRLLSRLVLLFLVASPTMPTFAIEEESRSTLHTYSP
                     ALRNDPSPFHCTDCWDLRPDQRSSLPQHQTQRSRRGHHDRGLRPHKNPFAKKSHSFSR
                     GLRSLPRHALSMGGFERTVEGWQVRTVDGDTIRYGTDRIRIRGYNAPELSEPGGQEAA
                     LRLEQLLQDGTINIIPHGHDVYGRTLADVFVNGQNVADVMMMEGFGRRG"
BASE COUNT          228 a          291 c          345 g          249 t
ORIGIN      
        1 cgtgagttcc ttgttaaaat agttgaaaga catcttgctg acgtacaggg cctcagcact
       61 attgcgccga tcgtggcgat aatcaaggtg ccaatctgtc tgcgaggagg tggagtttat
      121 caccatcgtc tcatggtaac gagagtggtc atagtgcctg agatcctgga cgaacgcttt
      181 tgactgtcct atcccctccg cccaaacccc tccatcatca tcacgtccgc cacgttctgc
      241 ccattcacga agacatcggc gagggtgcgg ccgtagacgt cgtggccgtg ggggatgata
      301 ttgatcgtcc cgtcttgtag tagctgctcc agccggaggg cggcttcctg cccgccgggt
      361 tcggacaatt ccggcgcatt atagccgcga atgcggatgc gatcggtgcc atatcgaatg
      421 gtgtcgccat cgaccgtccg cacttgccag ccttccacgg tccgttcgaa tcctcccatc
      481 gacagcgcat gtcgagggag cgacctcagc cctcgactga acgaatgtga tttttttgcg
      541 aatggatttt tgtgcgggcg tagccctcga tcgtgatggc cgcgtcgaga tcgttgggtc
      601 tgatgctggg gaagcgacga ccgttgatcc ggcctcagat cccagcagtc ggtgcagtgg
      661 aaggggcttg ggtcgttccg gagggcaggg gagtacgtgt gaagcgtgga ccggctttcc
      721 tcctcgatgg cgaatgtggg catggtgggc gaggccacca ggaacaggag gaccaacctg
      781 ctcaaaagac gggagtcggg tacgatgtag tgcagcatcc tgagagttct ccatggttcc
      841 ggagaagggc acagcaggct ccatgccacg agtcagtcca gacggtgtcg cggttgacaa
      901 gccgaaaaac cgcatgctca aatgcccgtc gaatctcatc ttttgtgaat cagagacgag
      961 gttcaccaga agcgctctca gcgggcgccg tcttttccga gaggtatgtg tgacaatcga
     1021 aaggttacgg aggtttaact catggatatg tttggaaaag ttggtctggt cgaggtgccg
     1081 atcctgatcg cgccagatca gaaggcgtgg atg
//
LOCUS       DASTWE010000522         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_466664, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000522 DASTWE010000000
VERSION     DASTWE010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466664"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFT30_05445"
     CDS             complement(<1..796)
                     /locus_tag="VFT30_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092107.1"
                     /translation="MRAWAGAPFPLGANWDGQGVNFALFSENATKVELCLFDRADDPN
                     PSVTIQVEEQTNQVWHIYLPEILPGQHYGYRVHGPYDPAQGHRFNPAKLLIDPYAKAV
                     AGTVQWSENRTFGYRIGDPEEDLSFDDRDNAAYVPKSVAVDSSFSWGADRLLNTPWDR
                     TVIYEVHVKGLTARHPEVPEALRGTYSGMTSPAILDHLKTLGVTAVELLPVHHFVRDQ
                     HLHDRGLTNYWGYNSIGFFAPDIRYAHSQKRGEQVREFKTMVKTLHS"
     gene            1841..2287
                     /locus_tag="VFT30_05450"
     CDS             1841..2287
                     /locus_tag="VFT30_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5092108.1"
                     /translation="MAHYITNIEAATLQNDMFRKVLFTAEHSQLVVMSLKPGEDIGTE
                     THQLDQFIRVESGRGTAYLNGAEYPLEDGTAIVIPSGTEHNIRNSGHEALKLYTIYSP
                     PEHKDGIVHATKRDTLGDREDHFDGKTTAMLQELSAASLVATAKVI"
BASE COUNT          551 a          610 c          651 g          527 t
ORIGIN      
        1 cactatgcag tgtcttcacc atcgtcttga attcgcgcac ttgctcgccg cgtttttgag
       61 aatgtgcata gcggatgtcg ggggcaaaaa atccaatcga attatagccc caatagttcg
      121 tcagtcctcg atcgtgtaaa tgttggtcgc gaacgaagtg atgaacgggc aacaactcaa
      181 cagcggtgac cccaagagtc ttgaggtggt ccagaatagc gggtgacgtc atacccgagt
      241 acgtcccgcg cagagcctcc ggcacttcgg ggtgacgggc ggtgagtcct ttgacatgca
      301 cttcgtaaat aaccgtccta tcccatgggg tgttgagaag cctgtcggct ccccaggaaa
      361 acgaagaatc aaccgcgaca cttttgggaa cgtaggcggc attatcccgg tcgtcaaacg
      421 acaggtcttc ttccggatca ccgatgcgat agccgaacgt ccggttttcg gaccattgga
      481 cagtgccagc gaccgccttc gcgtagggat cgatgaggag tttggccggg ttgaatcgat
      541 gaccttgcgc cggatcgtag gggccatgga cgcggtaccc ataatgctgt ccgggaagaa
      601 tttctggaag ataaatgtgc cagacctgat tggtctgttc ctccacctga atagttacag
      661 atggattggg atcatcagcc cgatcgaaca ggcaaagttc gaccttcgtc gcattttcag
      721 agaagagagc aaagttgacg ccttgaccgt cccaattcgc ccccagcggg aacggagctc
      781 cagcccatgc acgcattgtt tgcacctttc gacaaaatga atgcctgtca gcacgtgatg
      841 atatttatac atcttcgtaa aaccgcctgg cacgcgggtc gtggccaatt tatctggcaa
      901 aagccattcg ataccatgcc ctaaaggctg ataaaggcgt actatggatt tcactagtgg
      961 ggccgtcgca attatattgg taagacacac tagtgggccg ccctttcttg ggatgtaaat
     1021 atgctttcct tctcgcgttt gtagtggagt ggttgcactt ctcatgggat gtgtttcacc
     1081 gatcgcacgg caccacgccg tgctggctta tgatcgaagc gtcgaacggg tgaccaccga
     1141 acaactgctg gtgaacatta ttcgcgctca atcaccatcg ttccgatcga cggggaggag
     1201 ttcaccacgc gtttcctcag ccgctcgaag ccgccgtgcc ggtatggtat gtggaccgat
     1261 actacagtat ccggagatcg gtaccggatc gagcgagcga aatccgcaat gccaaggtct
     1321 tcggcatgtt gtatcagtca attgttccaa atgctggtgc agcctgtcag tgtgagtgcc
     1381 ccgggcatcg ccatcgcgaa ataacggcgc ggtcggccgc atggtgcctt tcggaagggc
     1441 cgtagcgtag atggatgagc acgtctatcg ccattggaca caaaagtatc gcaagccgct
     1501 ccgtccaccg gattcacata gcgggcgtac gttatgtcga tgttcatcat cccggcatct
     1561 ctcgacggtg ggggaggcac ctttgcttac ttccatcccg acggaaggca ggtacgggat
     1621 aaagaccttg atccgcattc ggtctctggc cattcctccg gcttggcaag acatatggat
     1681 ctgtcgcgat gcgtaagggt acatccaagg gaggagaccg tagcgatctc agggcgcgtt
     1741 tttcgtctcg tacaggctag ggagagcacg agtttcgaat ctgccggaat cccgttacaa
     1801 gtctgatcac cttccctttc cgagaggagg aacctcgacc atggcgcatt acattacgaa
     1861 catcgaagca gcgactctcc aaaatgatat gttccgcaaa gtcttgttca ccgcagagca
     1921 cagccagttg gtagtgatga gtctcaagcc gggggaagat atcggcaccg agactcatca
     1981 gctggatcaa ttcataagag tcgaatcggg tcgtggtacc gcttatctga atggcgcaga
     2041 gtatccgctt gaagatggca cggcgatagt cattccgtca gggaccgaac acaatattag
     2101 gaattccggt catgaggcct tgaagctcta tacgatctac agtccaccgg aacacaaaga
     2161 cggcattgtg catgccacga aacgggatac gcttggcgac cgagaggatc acttcgacgg
     2221 caagactacg gcaatgcttc aggagttgtc cgcggcaagt ctcgttgcaa cagcgaaggt
     2281 catatgaaat tgggcgtccg ataggacggc cattcggcca gtggccaagg agaaagctc
//
LOCUS       DASTWE010000523         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1436251, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000523 DASTWE010000000
VERSION     DASTWE010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436251"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFT30_05455"
     CDS             complement(<1..1230)
                     /locus_tag="VFT30_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5092109.1"
                     /translation="MQESRPFLVHGQWKSGELSAPVVDPFTGKLVTQVTRATESDVDE
                     AIASTCSAAATMEQLPAHARYNILQQVAALLYRRRDEFAQAITVEAGKPITDAKREVS
                     RAIQTFTVAAEEARRIPGEVVPLDWTPGFDTHLGILRRFPIGPILGITPFNFPLNLVA
                     HKVAPALASGNPILIKPAPQTPLTALLLGEVVMEAGIPPGGLNVVPCDNTLAERMVVD
                     PRFKLLSFTGSASVGWMLKAKCGKKKVTLELGGNAGVVIEPDADLELAAQRCAAGGFG
                     YAGQTCISVQRVFVHHSVADVFTTKLLMHVARLKVGDPSDETTSIGPLIDQAAAQRVE
                     SWIGEAVSDGARVLLGGKRMGSLVEATVLSNVKPDMKVSSKEVFGPVVTVTPYRQLSE
                     AVALLNQSDYGLQAGIFT"
     gene            complement(1265..1438)
                     /locus_tag="VFT30_05460"
     CDS             complement(1265..1438)
                     /locus_tag="VFT30_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092110.1"
                     /translation="MPDCPSCHGVSTYEGFTMEAIRDWGTEDLIAKAVAAQEAATVAQ
                     PAAETTAPVEATD"
BASE COUNT          368 a          501 c          461 g          345 t
ORIGIN      
        1 cgtgaagata ccggcctgca acccatagtc cgattgattg agcagcgcca cggcctcgct
       61 gagctgccga tacggcgtga cggtgacaac cggtccaaac acctccttag aggagacttt
      121 catgtcgggc tttacattcg acaacactgt ggcttcaaca agcgatccca tccgcttccc
      181 gcccaacagc acgcgcgctc cgtccgaaac ggcttcgccg atccagctct ccacccgctg
      241 agcggctgcc tgatcgatga ggggaccgat gctcgtcgtc tcgtcactag ggtcgcccac
      301 tttcaatcgc gcgacatgca tgagcaactt ggtcgtaaac acatcggcca ctgaatgatg
      361 aacgaagacc cgctgcaccg aaatgcatgt ttggccggca tatccaaatc cacctgctgc
      421 acaacgttgg gctgcaagct cgagatcggc atcgggctcg ataaccacgc cggcattgcc
      481 cccgagttca agcgtgactt ttttctttcc acattttgcc ttcagcatcc atcctaccga
      541 cgcacttcct gtgaaactga gcaatttgaa tcgtggatcg acaaccatcc gttcagcgag
      601 ggtgttatcg catggcacca cattgagccc gcccggagga attcccgcct ccatgaccac
      661 ctctccaagg agaagagccg ttaacggcgt ctgaggagcc ggtttgatca gaatcgggtt
      721 gccggaagcg agcgcaggtg ccaccttgtg cgccacaaga ttcagcggga agttgaaggg
      781 ggtgatgccg agaatcggac cgatcgggaa acggcgaagt atgccaagat gggtatcgaa
      841 gccgggcgtc caatcgagcg ggaccacctc cccaggaatc cgtcgtgctt cttcggcagc
      901 caccgtaaag gtttgaatgg cccgactgac ctcccgcttt gcgtcagtga tcggcttgcc
      961 cgcctcgacc gtgatggcct gagcaaattc atctcgacgc cgatagagca gggcggcgac
     1021 ctgttgaagg atattgtatc tggcatgtgc cggaagctgc tccatcgtcg cagccgcact
     1081 gcacgtggac gcgatggcct cgtcgacatc agattcagtc gctcgggtga cctgggtaac
     1141 gagttttccg gtgaacggat caacgacagg agccgagagt tcgcccgact tccattggcc
     1201 atgaacgagg aatggacggg attcctgcac tggaaggctc gctggagtct aggatgagat
     1261 tgtattagtc cgtcgcttca accggcgcag ttgtttcagc ggcaggttga gcaaccgtgg
     1321 ctgcttcttg tgcggcgaca gccttggcaa tcaggtcttc ggtgccccaa tcgcggatcg
     1381 cctccatagt aaatccctcg taggtcgaca caccgtgaca agagggacaa tccggcatcg
     1441 gaacctttct gtagaagact tccgtgaggc aagacataca gacccagaag tcgtcatcgc
     1501 gatgagagac gggccaaaac gattgcatcg aagccatatg gacaccctac ctttattgac
     1561 gaagaggact atcgtacctg cgtgccgatg tcaacatcac gcgcaacggc tcgccccgct
     1621 ccggcgcaat tcagctattt cccgtcattc gccagcagtc gatcgatctc ttctg
//
LOCUS       DASTWE010000524         1239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_692995, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000524 DASTWE010000000
VERSION     DASTWE010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692995"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1190
                     /gene="wecB"
                     /locus_tag="VFT30_05465"
     CDS             84..1190
                     /gene="wecB"
                     /locus_tag="VFT30_05465"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU5092111.1"
                     /translation="MKVKLIDIIAGARPNFMKIAPIIEALITAERRGGQLRYRLIHTG
                     QHYDRAMSGSFFEELGIPDPDINLEVGSGTHAEQTAAIMVGYEKVLVKKRSDLCLVVG
                     DVTSTMACSIAARKLGVPVAHVEGGIRSGDWTMPEEINRVVTDSITNWFFTTSETANH
                     NLRRAGVTDDQIFFVGNTMIDTLLKNVPRLRPPACWHSLKLEPGQFFVVTLHRPANVD
                     GEQQLLSLLRAIAEGTKGLPVIFPVHPRTAKNLRELDSAIPQLHYVDPLGYLEFNYLV
                     KHAKGVITDSGGITEETTVLGVPCLTLRDNTERPETITIGTNELIGTDPRKLAPALSR
                     LMAGQWKKGAIPPLWDGKAAERIVGQLERLLLES"
BASE COUNT          304 a          318 c          340 g          277 t
ORIGIN      
        1 attcagttgc cgcgtgtact tcgagccaat accatcacag aaaccttcca aggtagatta
       61 acaggaatca agtttgagtc gaggtgaaag tgaagctcat cgatatcatc gccggggcgc
      121 ggcccaattt catgaaaatt gcgccgatta ttgaagcgct aataacagcc gagcgccgtg
      181 gcggtcagct ccggtaccgg ctgattcata cgggtcaaca ttatgatcgt gcgatgtcag
      241 gaagtttctt cgaagaacta gggattccag atccggatat caatctcgag gtggggtctg
      301 gcactcatgc cgaacaaaca gccgccatca tggtcgggta tgaaaaagtt ttagtgaaga
      361 aaaggagcga cctctgtctt gtcgtcggag atgtaacgtc cactatggcc tgttcaatcg
      421 cggcgcgaaa acttggcgtg ccggtagccc atgttgaggg cggtattcgg tccggtgatt
      481 ggacaatgcc ggaggaaatc aatcgggtgg tgaccgactc catcaccaat tggtttttta
      541 cgacgagcga aacagccaac cacaatttac gccgtgccgg tgtcactgat gaccagatct
      601 tttttgtcgg caatactatg atcgatacgt tgctcaagaa cgtgccgcgc ttgcgacctc
      661 cggcttgttg gcattccctc aagctcgaac ccggacagtt ttttgtcgtg acactccatc
      721 gaccggcaaa cgtcgatgga gagcagcagc tcttgtcgtt gctccgcgcc atcgccgaag
      781 gcactaaagg acttccggtg atatttcccg tgcatccacg gaccgcgaag aatctcaggg
      841 aactcgacag tgcgataccg cagcttcact acgtcgatcc gcttggctat ctggaattca
      901 actatctcgt caaacatgcc aaaggagtga ttactgattc gggtggcatc accgaagaaa
      961 caacggtcct tggagtgccg tgcctgaccc tccgcgataa cactgaacga cctgagacca
     1021 ttacgattgg caccaacgaa ttgatcggga ccgatccgcg gaaactcgcc ccggcactgt
     1081 cgcggttaat ggcagggcag tggaagaaag gcgcgatccc accgctgtgg gacgggaaag
     1141 cggcggagcg gattgtcgga cagttggaaa ggcttctgct cgaatcgtga gttcgttctg
     1201 agactaggaa cagacggaaa ccttgctcaa atgtctatt
//
LOCUS       DASTWE010000525         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1565440, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000525 DASTWE010000000
VERSION     DASTWE010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565440"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFT30_05470"
     CDS             <1..894
                     /locus_tag="VFT30_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092112.1"
                     /translation="AFALLLCSASYVGNAQEAKFVEREVTFKTEDGWVIHGVLTTPPA
                     LQPGEKIAGAVLVPSSHHDRDIYGHNGYPSIREVLEKAKIATLRFDIRGRGTSAEPRD
                     YWDFTEDQKARVSLDVAAAIEFLCQQNGIDQSRIGIVTENESADAAVIAATKDARIRA
                     MILLSGRMGQTAKDGIKSRQDLAILGVVSKEDRVSFADLADAYRLSPDPASSLMIHDN
                     IGLGNAMFIMNMAKHPNDKLEVTVGEWLVGQLKLSSESREVTFQSSDGWKLSGTLRIP
                     ANVGPTGAPGIVFVHSNLSDR"
BASE COUNT          249 a          253 c          307 g          236 t
ORIGIN      
        1 gcattcgcct tgttgctctg ctctgcttca tacgttggga atgcgcaaga agcgaagttt
       61 gtcgaacgag aggtgacttt taagactgag gacgggtggg tgatacacgg cgttctcact
      121 acgcctccag cccttcaacc gggagagaaa atagcaggcg cagttctggt tccgtcgtct
      181 catcacgatc gcgacatcta tggtcacaac ggatatccca gcattagaga ggtgcttgaa
      241 aaggcgaaga ttgcaactct ccgatttgat attcgcggac ggggcacgag tgctgagccg
      301 cgagactatt gggacttcac tgaggatcaa aaggcccgag tcagtctgga tgtggctgcc
      361 gcgatcgaat ttctttgtca gcaaaacggt atcgaccaaa gtcggatagg cattgttacg
      421 gaaaatgaga gtgccgatgc agcagtgata gcggcgacga aggacgcgcg catacgcgca
      481 atgattttgc tctcgggtcg aatgggacag acggcaaagg atgggatcaa gagtcgccag
      541 gacctcgcca tacttggcgt ggtgagtaaa gaagaccggg tgagctttgc agatctcgct
      601 gacgcatatc gactctcgcc cgatcccgct agcagcctga tgattcatga caacattggc
      661 ctggggaacg caatgttcat catgaacatg gccaagcatc ccaacgacaa actggaggtg
      721 accgttggtg aatggctcgt tggccaactc aagctttcgt cggaatcgcg cgaagtaact
      781 ttccaatcga gcgatggatg gaagctttcc ggtacgcttc ggatcccggc aaacgtgggt
      841 ccgactggtg ctccgggcat tgtattcgtg cactcaaatc tgagtgatcg ctaggtcttt
      901 gatcatcttg agaagtcgtt ggcagctgcc ggcttcgcga ctctcaattt cgactttcgc
      961 ggccgaggca agagtcggag caaggggtca tattttgatc tgtcgcaagc ggagaaagac
     1021 aaaggctacc tcgatgttac cgctg
//
LOCUS       DASTWE010000526         1395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1016419, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000526 DASTWE010000000
VERSION     DASTWE010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016419"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(388..>1395)
                     /locus_tag="VFT30_05475"
     CDS             complement(388..>1395)
                     /locus_tag="VFT30_05475"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEU5092113.1"
                     /translation="NEEGRLLIAEQCEQARITMEQVLEFLLHHIEMVVFFTVLAEQIG
                     LPLPAIPILLAAGAVAGDGQASLTLVTALSVAACLLGDMVWFELGRHRGRQTLTLLCR
                     IALEPDACVRRTENFFLIHGIRALIYAKFIPGLSTLAPALAGLFKIPTGRFLVFNGVG
                     ALLWSFSFLMLGYVFSDQIGYVAELAVQFGKTATTIIIAGVAGYLLYKYVHRQRLLRE
                     LRVARISPTELKQLMDDGHQSVIVDLRGTLDHVADPYTIPGALRIAAEELEIRHYDIP
                     RDRDVILFCACPNEATAARMALMLRRKGITRVRPLTGGIDLWRDLAYPLEMVVSKEGL
                     Q"
BASE COUNT          341 a          369 c          382 g          303 t
ORIGIN      
        1 tgccagcgaa cggttgcatc accatgactg gccggttgat tttgaaccgt cacatcccag
       61 gatgggaatc ttagtgaaag aagtatccga gcaagcttcc agcattctcg accgaaaacg
      121 atagagtcag ttaattatat gtttcgatcg ggcgctgcta tggttctgag aactactgtt
      181 aatggttccg atatccgata acattttgtg taatgacgac cagcaaacaa gcaaatcgca
      241 ggtcgttgat ctgcctatcc tgacctattg tgatggggga gtagacggca agaggctaaa
      301 taacaagagc taagactttg ggcccttttg ctctgattcg gacggacccc aaaagatcgg
      361 tcttattcaa ccttggtagc ccagcagtta ctgcaatcct tccttagaga ccaccatctc
      421 cagcggatag gccaaatcac gccacaaatc gatgccacct gtgagcggcc ggactcgggt
      481 aatccctttt cgtctcaaca tcaatgccat ccgcgcagca gtggcttcgt ttggacaagc
      541 acagaacaag atgacatctc ggtctcgtgg aatatcgtaa tggcgtatct caagttcttc
      601 ggccgcgata cgcagtgctc ccgggatcgt ataggggtcg gcgacatggt ccaatgtgcc
      661 gcgcaagtcg acgataaccg attggtggcc atcgtccatc agttgcttga gctcggtcgg
      721 actgattcga gccactcgaa gctcccgcaa gagccgttgt cggtgtacat acttgtagag
      781 aagatagcct gcgacgcccg caatgatgat cgtcgtcgca gtctttccaa attgtaccgc
      841 gagttcggcc acatagccga tctgatcgct gaacacatac ccgagcataa gaaatgagaa
      901 gctccagagc agcgcgccga caccattgaa gaccaggaat cgtccagtcg ggattttaaa
      961 gagcccggct agtgcggggg ccagtgtgct caagcccggg ataaacttgg cgtagataag
     1021 cgcccggata ccgtggatca gaaagaaatt ctctgtgcgc ctgacacagg cgtcgggttc
     1081 cagagcgatg cggcagagca gggtcagcgt ttgtcgcccg cgatgacggc ccagctcgaa
     1141 ccataccatg tcaccgagca ggcatgcggc aacggagagg gcggtgacca gggtcaggct
     1201 tgcctgtcca tcaccagcga ccgccccggc ggcgagcagg atgggaatcg cgggcagggg
     1261 aaggccgatt tgctctgcga ggacggtgaa aaacaccacc atctcgatat gatggagcaa
     1321 aaactccaga acttgttcca tggttatcct ggcttgctcg cattgctctg cgattaaaag
     1381 tcggccttct tcatt
//
LOCUS       DASTWE010000527         1118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1274704, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000527 DASTWE010000000
VERSION     DASTWE010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274704"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1118)
                     /locus_tag="VFT30_05480"
     CDS             complement(<1..>1118)
                     /locus_tag="VFT30_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HEU5092114.1"
                     /translation="KSGPTRLGFAVLLKYFQYEGRFPQHPREVPSEIVIYMAQQLDLE
                     PESWSDYGWYGRAIKYHRAQIRQELGYREATVADGEALITWLGKEVLPTTHRPEHLKE
                     ALYQRCREIRIEPPTPERVERLIRSALQQFEHQLGERVLHRLSPATQKKLDALLTADE
                     SKEDDTQTPETPDSGPAVLHDLRADPGRPSLDNLLREMTRLERVRDLDLPLDLFDHLG
                     PKMLQAYRQRAAVEAPYELRRHPEALRMTLLSVFGHCRQRELTDTLVDSLIELIHRIG
                     AKAERKVEKELLEDLKRVNGKTGMLFRLAEAALENPNGVVKEIVFPVVNEDTLRDLVK
                     EWKSTGPIYRYHVQTVIRSSYQSHYRRMLPKLLQTLEFR"
BASE COUNT          242 a          305 c          316 g          255 t
ORIGIN      
        1 cgaaactcta gggtttgcag tagcttgggc aacatccggc gataatgcga ctgatatgaa
       61 ctgcggatga cggtctgcac atggtaacga tagatcggcc cagtcgattt ccactctttg
      121 acgagatcac gcaaggtgtc ctcattcacc actgggaaga caatctcttt gaccacgcca
      181 ttcgggtttt caagcgccgc ttcagccagg cgaaacagca tcccagtctt accattgacc
      241 cgtttgagat cctccaacag ctctttttcg accttgcgtt cagccttggc gccaatgcga
      301 tggatcagct caatgagcga gtccaccaag gtatcggtga gctcacgctg ccggcagtgg
      361 ccgaaaacag ataacaaagt catacgcagc gcttccggat gccgacgcag ttcataaggg
      421 gcttcaaccg cagctcgttg ccggtaagct tgaagcattt tgggacccag atggtcaaag
      481 agatcgaggg gcaggtccag gtctcgcacc cgttccagcc gggtcatttc gcgtagaaga
      541 ttatccagac ttggacgccc gggatcggca cgtaggtcgt gcaacaccgc ggggcctgaa
      601 tcgggtgtct caggcgtttg ggtgtcgtct tccttggatt catcagcagt caacagggcg
      661 tcgagtttct tctgggttgc tggcgatagg cgatgcaaga cccgctcgcc cagttggtgt
      721 tcgaactgtt gaagggccga tcggatcagg cgttcaacac gctctggtgt cggtggttca
      781 atgcgaatct cacgacatcg ttgatagagc gcctctttca aatgttccgg acgatgcgtg
      841 gtggggagaa cctccttgcc aagccaggtg atcagcgcct ctccatcggc cacggtagct
      901 tcacgatagc caagctcttg gcgaatctgt gcacggtggt atttaatggc acgaccgtac
      961 caaccgtaat cactccagct ctcgggctcc aaatcgagtt gctgtgccat atagatgacg
     1021 atctcggatg gcacctcgcg agggtgttgg gggaaccgcc cttcatactg gaaatacttt
     1081 agcagtacgg cgaaccccag gcgagttggg ccagactt
//
LOCUS       DASTWE010000528         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_305182, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000528 DASTWE010000000
VERSION     DASTWE010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305182"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFT30_05485"
     CDS             complement(<1..1081)
                     /locus_tag="VFT30_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5092115.1"
                     /translation="MRIAQVAPLWESVPPKLYGGTERIVSYITEELVALGHDVTLFAS
                     GDSETTARLEAICPQALRLNTGIFNRDAPMMMLQERGLGAASGFDIIHSHLDFIGFPL
                     ARRNTIPVVTTLHGRQDLPELEPVFREFSEMALVSISDAQRKPLPWANWVGTVHHGLP
                     RNLYSLHPQAQGYLAFLGRISPEKRPDQAIEIAKRVGLPLRIAAKVDPADQNYFEAEI
                     EPLLNHPLIEFIGEISDAEKDEFLGNAMALVCPYDWPEPFGLVLIEALACGTPVIAYR
                     RGSIPEIIDHGVTGFICETVSEMVDVVGQVSLIDRRRCRAAFDERFTADRMARDYVTL
                     YERVLLEDGAVQATQAFASEQHKILG"
BASE COUNT          277 a          316 c          328 g          275 t
ORIGIN      
        1 gcccaagaat cttatgctgc tcggaggcga aggcctgtgt ggcttgaaca gctccatcct
       61 cgagaagaac acgttcatat agggtcacat agtcgcgtgc cattcgatcc gcagtaaacc
      121 gctcgtcaaa ggcagccctg cagcgccgac gatcgatgag tgagacctgt ccgaccacat
      181 ccaccatttc ggatacagtc tcgcaaataa agccggtgac gccgtgatcg atgatttcag
      241 gaatcgagcc acgtcggtag gctatcactg gggttccaca ggccagagct tcgatcaata
      301 ccaacccgaa gggctccggc caatcgtagg ggcacaccaa tgccattgca ttgccgagaa
      361 actcgtcttt ctcggcatcg gaaatctctc cgatgaattc aatcaaggga tggttgagca
      421 acggttcaat ttcggcttcg aagtagttct gatcggcagg atcgactttt gccgcaattc
      481 gcaggggaag gcccacgcgt ttcgcgattt caatcgcttg gtccggacgt ttttcaggag
      541 agattcgtcc aagaaaagct agataccctt gggcctgggg atgaagggag tagaggttgc
      601 gaggaaggcc atgatggacg gtccccaccc agttggccca aggcagaggt ttgcgctggg
      661 catccgaaat ggataccaag gccatttcag aaaattcacg gaagaccggc tccaattccg
      721 gaaggtcctg acgaccgtga agggtcgtca ccaccggaat ggtattcctc cgtgccagcg
      781 ggaatccgat gaaatccagg tgtgaatgaa taatatcgaa gccgcttgcc gctccaaggc
      841 ctcgttcctg aagcatcatc atgggggcgt cccgattgaa aataccggta ttcaaccgga
      901 gggcctgagg gcagattgct tcaagccgtg cggtcgtctc cgaatcaccg ctggcaaaca
      961 gcgtaacgtc atgtcccagg gcaaccaatt cctctgtaat gtacgaaacg attcgttcgg
     1021 tacctccgta gagtttgggc gggacactct cccacaacgg agccacttgt gcgattctca
     1081 tggttttcct ttcttccctt tgtcacttga ttgcttggtt cagtcgccag cttgtttgtg
     1141 gttgattcta catgggccgg tttgaaagca attgcccgtg atcggttgga agttac
//
LOCUS       DASTWE010000529         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_434657, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000529 DASTWE010000000
VERSION     DASTWE010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434657"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1091
                     /locus_tag="VFT30_05490"
     CDS             <1..>1091
                     /locus_tag="VFT30_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5092116.1"
                     /translation="TMRTFHIGGTASRVSEQSTLDAKNDGVLKFINLTSVQNKAGDLV
                     VMNRNGLIAIVDEKGREKEKYSVVYGAKIKFKEGDDVKEGDSLVEWDPYTYAILTEVG
                     GTVQFKDLVDAVTMTEEVDEVTSMSRWVVTQSQDEKRQPQIVIKSSDKHKTVKKYLMP
                     ERAHLMVQDGDELNPGDVLAKIPRETTKTKDITGGLPRVVELFEARKPRETAVITHID
                     GVVKYGDIAKGTRKILVTNENGMEREYSLPRGVHVNVQEGETVRAGEPLMDGPLNPHD
                     ILEVLGEKELQAYLVNEIQEVYRLQGVNINDKHIEVIVRQMMRWIRVEEVGDTDFLLE
                     EQVDKFRFKEENDRVLGEDGRPARGEPLL"
BASE COUNT          293 a          262 c          325 g          211 t
ORIGIN      
        1 tgaccatgcg caccttccac atcggtggca cggccagtcg agtatcggaa cagtcaaccc
       61 tcgacgccaa gaacgatggc gttttgaagt tcatcaatct cacatcggtt cagaacaagg
      121 caggcgatct cgttgtgatg aaccgcaacg gcttgattgc catcgtcgac gaaaaggggc
      181 gtgagaaaga aaagtactcg gtcgtttacg gcgccaagat caaattcaag gaaggcgacg
      241 acgtcaagga aggcgacagc ttggttgaat gggatccata cacctatgcg attttgacgg
      301 aggtgggcgg cacggttcag tttaaagatc tggtggacgc tgtcaccatg actgaagaag
      361 ttgacgaagt caccagcatg tcacgctggg tggtgacgca gtcacaagac gaaaagcggc
      421 agcctcagat cgttatcaag agttctgaca aacacaagac ggtcaagaaa tacctgatgc
      481 ccgagcgtgc gcacttaatg gtgcaggatg gcgacgaact gaatccaggg gacgtcttgg
      541 caaagatccc gcgcgaaacc accaagacga aggacatcac tggaggtctg ccccgcgtcg
      601 tggagttgtt cgaagctcgc aagcctcgcg agaccgcagt gatcactcac atcgatggag
      661 tggtcaagta cggcgacatt gccaaaggga cccgcaagat tctggtgacc aacgaaaacg
      721 gcatggaacg tgagtactcc ctgccacgtg gtgtgcacgt caacgttcag gaaggcgaaa
      781 ccgtacgtgc cggcgagcct ttgatggacg ggccgttgaa tccacatgac atcctggaag
      841 tcttgggcga gaaggaactg caagcgtatc tggttaacga aatccaggag gtttatcggt
      901 tgcagggtgt gaacatcaac gacaaacaca ttgaagtcat cgtgcgtcag atgatgcgtt
      961 ggattcgagt cgaagaagtg ggcgatacgg atttcttgct cgaagagcaa gtggacaagt
     1021 ttcgtttcaa ggaggaaaac gatcgtgtcc ttggcgaaga cggccgtccc gcacggggag
     1081 aacccctctt g
//
LOCUS       DASTWE010000530         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1468843, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000530 DASTWE010000000
VERSION     DASTWE010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468843"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..947)
                     /gene="ttcA"
                     /locus_tag="VFT30_05495"
     CDS             complement(90..947)
                     /gene="ttcA"
                     /locus_tag="VFT30_05495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEU5092117.1"
                     /translation="MPKLDEATEKIQTRLCRLVGQAIADYRLIEDGDKVMVCLSGGKD
                     SYGLLDILLVLQRRAPIRFDLIAVNLDQRQPGFPEHVLPQYLTSRGIPFHIESRDTYS
                     IVKRLIPEGQTTCSLCSRLRRGHLYRIATELGATKIALGHHRDDIMETLFLNLFYTGK
                     LKAIPPKLRSKDGRHVVIRPLALVKEIDLARYAELRGFPIIPCDLCGSQEDLKRKKVK
                     TFLQQWERESPGCSDSITAALGNVAPSLLMDSRLFDFKSLTTAADNLAEGDAWLDEEP
                     SNRKDARPS"
     gene            1046..1561
                     /locus_tag="VFT30_05500"
     CDS             1046..1561
                     /locus_tag="VFT30_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEU5092118.1"
                     /translation="MVQSILFVCTGNVFRSMAAEYALRAQLGHQSSYRIESAGIEAKA
                     NSIHPIISKRLIQRGTDPSAHIPQRLTKKLIDRNDLIIAMGLGHREFIHKQFGLNVPL
                     FNEVSFGEEIPILDLHEALPDWERDIDEARAYVESVIDHIWESVPALLARLPHFQGSR
                     RPMKRWCPFTR"
     gene            1688..>2718
                     /locus_tag="VFT30_05505"
     CDS             1688..>2718
                     /locus_tag="VFT30_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEU5092119.1"
                     /translation="MDRTVSDVLKTPVTGQDDPQWASWSDEALLDLPMCDLHVGLKGG
                     VLERPIAELTEELERCGLLFRPHFWLSNEWFTPDGVPGIAVPFYLAHPRLAKLEQAQM
                     LEVEGGTTEWCLRILRHEAGHAIENAYKIRRRKTRQDIFGKSSQQYPMYYSPRPYSRS
                     FVRHLDLWYAQSHPDEDFAETFAVWLTPDSLWEERYRGWPVLKKLKYVDALMKELRGV
                     LPSVTTCEEVDPLSNLKKTLREHYERKRRHYGIERQLQYDPDLKRLFSSLPNHASKQS
                     AATFLNRFRREVRRKVASWTGEYQYTINQVLEDMIQRCRELNLRVPVAEDQAKLDFTI
                     LLTVHTMNF"
BASE COUNT          639 a          756 c          708 g          615 t
ORIGIN      
        1 caccgctggc cgaaaccccg gttctagctc gcaatacagg catggactga tctcactcag
       61 tcgaattatc ctccttcagg gtcgttcaat caagaaggac gggcatcttt tcggttcgac
      121 ggttcttcat ctaaccaggc atcaccctcg gccaggttgt ccgctgccgt cgtcaatgac
      181 ttgaaatcaa agagccgaga atccatcaaa agcgatggcg cgacattgcc gagagcagcg
      241 gtgatgctgt cagaacaacc aggcgactcc cgctcccact gctgaagaaa cgtcttcact
      301 ttttttcgtt tcaaatcctc ttgagagccg cagaggtcgc agggaatgat tggaaatcct
      361 cgcagctccg catatcgtgc gaggtcgatc tctttaacga gcgccagcgg tcggatgacg
      421 acatgtcgcc cgtcctttga acgaagcttg ggagggatcg ccttcagctt gcccgtatag
      481 aaaaggttca agaacaaggt ttccatgatg tcgtcgcggt ggtgaccgag cgcgatcttc
      541 gtggcgccaa gctccgtcgc aatgcgataa aggtgtcctc gtcgtaaccg tgaacacaac
      601 gaacaggtcg tctgaccttc cgggatgagc cgcttcacga tcgagtaggt atcacgggat
      661 tcaatatgga acggaattcc gcggctagtc agatactgag ggagcacatg ttccggaaaa
      721 cctggttgtc gttgatcgag gttgaccgcg atcagatcaa accgaatcgg tgcgcgccgt
      781 tgcaatacga gcaagatatc caataaacca taactatctt taccgcccga gaggcacacc
      841 atcaccttgt cgccgtcttc aatgagccgg tagtcggcga tggcctgacc caccagccgg
      901 caaaggcgcg tttgtatctt ctccgtggct tcgtctaatt taggcattga tattggggta
      961 agcgggacga tcatggcaag attgtagcgg ctccattatg atcctgtcga caggatcaca
     1021 taagaaaaag gagagcgaac cgtctatggt gcagtctatt ctctttgtat gtaccggaaa
     1081 cgtcttccgt agtatggcag ccgaatatgc gttacgggca cagctcggtc atcaatcgtc
     1141 ctaccgaatt gaatctgcgg ggatcgaagc aaaggccaac tcgatccacc ccatcatttc
     1201 caagaggctg atccaaaggg gaaccgaccc ttccgcgcat atcccgcaaa ggctgactaa
     1261 gaagctgatc gaccgaaatg acctcatcat tgcgatgggg cttggccacc gcgagttcat
     1321 ccacaaacaa ttcgggttga atgttccgct cttcaacgag gtgtcgttcg gcgaggaaat
     1381 cccgatcctc gacctgcacg aggccctccc tgattgggag cgtgatattg acgaagcacg
     1441 ggcttatgtg gagtcggtca tcgaccacat ttgggaatcc gtaccggcgc tgctagcacg
     1501 actcccacat tttcagggaa gtcgacggcc gatgaaacgg tggtgtccgt tcacacgata
     1561 actgtctgca tgattttctg atcgtctgtc tctacctccc cgacacgcgc tccatcagac
     1621 agtctaaaga ctgcatgcta tatttccatg gacggcacaa taggcgtatt tcaaaggttt
     1681 gcctttcatg gaccgcacag tttccgatgt actgaagaca cccgtcaccg gacaggacga
     1741 tccccaatgg gcctcttggt cggacgaggc gctgctagat cttccgatgt gcgacctgca
     1801 cgttgggctc aagggcggag tccttgagcg acccatcgct gagttaaccg aagaactgga
     1861 gcgctgcgga ttgctgtttc gtccgcactt ctggctctcc aatgagtggt ttactcccga
     1921 tggagttccc ggcatcgccg tccctttcta cctggcccat cctcgtctgg caaaacttga
     1981 acaagcccaa atgttggaag tggaaggtgg gaccacggaa tggtgtttgc gcattcttcg
     2041 ccacgaagct ggccatgcga tcgaaaatgc ctacaaaatc cgccggcgca aaacgcgcca
     2101 ggacattttc ggtaagtctt ctcaacagta cccgatgtac tactcccctc gtccctatag
     2161 ccgaagtttt gtccgccact tggacttgtg gtatgcccaa agccacccgg acgaagactt
     2221 tgctgaaacc ttcgcggtct ggttgacgcc cgactccctg tgggaggaac gataccgggg
     2281 gtggccggtg ctcaagaagc tcaagtatgt cgatgcactc atgaaggagc ttcgaggggt
     2341 cttaccctcc gtgactacct gcgaagaggt cgatcccctt tccaatttaa aaaagaccct
     2401 tcgcgaacac tatgaacgca agcggagaca ttatggaatc gaacgacaac tgcagtacga
     2461 cccggactta aaacggctgt tttctagttt gccgaaccat gcgagcaagc agagcgctgc
     2521 caccttcctg aaccgctttc gtcgagaagt gcgacggaag gtcgccagtt ggaccggcga
     2581 gtaccaatac acgatcaacc aggtgctgga agatatgatt cagcggtgcc gtgagctaaa
     2641 ccttcgcgtt cccgtcgcgg aagaccaagc caagctcgat ttcaccatcc ttctgaccgt
     2701 tcacactatg aatttctt
//
LOCUS       DASTWE010000531         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_919436, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000531 DASTWE010000000
VERSION     DASTWE010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919436"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFT30_05510"
     CDS             <1..483
                     /locus_tag="VFT30_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5092120.1"
                     /translation="LVWGVPKKGRGLIELAIGRDVKDRKKFSSRTTSPKESVTEYVVE
                     RRYGKAAAHVLLYPRTGRTHQLRVHLASLGHPILGDHTYGGRKVCTIGEVEIPRVMLH
                     ARTLGFTHPATGNVENFTRSVPGDMERVMDALERIIIPGEKKLPRGPVAEMYVAEDAC
                     "
     gene            477..899
                     /locus_tag="VFT30_05515"
     CDS             477..899
                     /locus_tag="VFT30_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092121.1"
                     /translation="MLTTELLDGVGGLTSHLKSYAAKLPTVIHLSALPSGLKPSFEAV
                     DSYETIVSRIRNQSAGTPYKVLNESLVSSLEAFEMGNLLGAVQPLPAALDHLERMQLD
                     KEIEVGRMDDKRLKEYRAALHKVLPGNKPELDGAGGGM"
     gene            1174..1605
                     /locus_tag="VFT30_05520"
     CDS             1174..1605
                     /locus_tag="VFT30_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5092122.1"
                     /translation="MPPTLKSAVTYSHDEPPDASQLLGLFHQAPWAKGRSLDDAKEML
                     RHTDLTLCAWDGDRLIGFGRVLTDFIYRATIWDVIVDHAYQKQGIGTELVQRILNHPR
                     LKKVELFWLCTRRSGFYEKLGFSSKEQTGMVWSRSKQARSE"
BASE COUNT          408 a          470 c          499 g          395 t
ORIGIN      
        1 ctggtctggg gcgtgcccaa gaagggccga ggattgatcg aactggctat cggccgggac
       61 gtcaaagacc ggaagaaatt ttcctcgaga acgaccagtc ccaaggaatc cgtgacggag
      121 tacgtagtgg agcggcggta cggaaaagcg gcggcccatg tgctcctcta cccgcggacg
      181 ggtcgaaccc atcagcttag ggttcacctc gcttccttgg gacatcccat tttgggggat
      241 cacacgtacg gtggacgaaa ggtgtgtacc atcggtgaag tggagatccc tcgggtgatg
      301 ctccatgcca gaaccctcgg cttcactcat cctgcgacag gcaatgtgga gaacttcacg
      361 aggtcggtgc ctggcgatat ggagcgggtg atggatgcgc ttgaacggat catcatccct
      421 ggcgagaaga agctgccgcg tggaccagtc gctgagatgt acgtcgccga ggatgcatgc
      481 tgaccaccga actactcgat ggggtgggag ggttaacgtc tcatctcaaa tcctatgccg
      541 ccaaacttcc gaccgttatc cacttgagtg cgctgccctc gggtctgaaa ccttctttcg
      601 aagcggtcga tagctacgaa acaatcgtat cacgtattcg aaaccagagt gccggaaccc
      661 catataaagt gctgaatgaa tctctagtgt cctccctgga ggcgtttgaa atggggaacc
      721 tcctcggcgc tgttcaaccg cttcctgcgg cattggatca cttggaacga atgcagctgg
      781 ataaagaaat tgaggtcgga cgaatggacg acaagcgact caaggagtac cgcgccgccc
      841 tacacaaagt ccttccaggc aataaacctg agttggacgg cgcgggtggc ggcatgtaat
      901 gccgcagtgt tgagttctga gcgctaaaaa ctcagtaaga cggctagtgg cccatcttgg
      961 gacccaacgc gttcgcgcca acggtgacga gtttgaagcg ggcagcgtta ccgcagtgtg
     1021 ggcacttcgc tcggacggtt tcatcatcga gcacttcgta ctcatccgct ttcagccaca
     1081 tgagttggaa gcactttgga caggttcgga cttgcgttga catcggtttc cccgtacact
     1141 ccaagacaat tcgggaggat actctagtat aagatgcctc caaccctcaa gtctgccgtc
     1201 acttattccc atgacgagcc tcctgacgcg tctcagctcc ttgggctatt ccaccaagct
     1261 ccatgggcca agggccgatc tctcgatgat gcgaaggaaa tgctacgtca taccgacctg
     1321 acactctgtg cttgggatgg agatcgtctc atcgggttcg gtcgagtact caccgacttt
     1381 atctatcgtg caacgatctg ggatgtgatc gtcgatcacg cgtatcaaaa acaggggatc
     1441 ggtacggagc tcgttcaacg cattctcaat catccccgac tcaagaaggt cgaactcttc
     1501 tggctctgca ccagacggtc aggattttac gagaaattgg ggtttagctc caaagaacag
     1561 accgggatgg tctggagccg aagcaaacag gctcgctcgg agtaatacgg cgtatgcgtg
     1621 tcttcagttt ggcgattgcc ctctttctcg caacagttgc tggatgtttc ccgactactc
     1681 atgacaaaaa tgggattccg tacagccggg ttcccggtgg agtcgagtgt tcatggatga
     1741 ctcagaaaac aatcggttgt ccggaaatta tc
//
LOCUS       DASTWE010000532         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_725329, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000532 DASTWE010000000
VERSION     DASTWE010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725329"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFT30_05525"
     CDS             <1..317
                     /locus_tag="VFT30_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5092123.1"
                     /translation="GGDLPPSMRRVPGSVWLIADSSSPVGLSVAKDVIQRGHRVVLAA
                     RNMAAMQDLVRGFPDTTFVVALDGTSTEDITRVVQESKGRFGPIEVLVNTPGVGSLPS
                     LG"
     gene            complement(354..>1067)
                     /locus_tag="VFT30_05530"
     CDS             complement(354..>1067)
                     /locus_tag="VFT30_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5092124.1"
                     /translation="AARLDETGPTEVAYAATAFNTMQDRITHYLKERVQILAAISHDL
                     QTPITRMKLRAEVAEDGPEKEKLISDLSEIERLVREGVAYARTAHGNVENPARIDIGS
                     FVESIVFDYQDTGKAVKTARMIEAVVTTRPHALRRILTNLIDNALKFAGEAEVEIIRT
                     KDGKICIAVCDRGPGIPDGQLEAVLQPFYRLEQSRNRDTGGTGLGLAIAQQLTLAIGG
                     SLSLKNRSGGGLAAEIVLS"
BASE COUNT          202 a          313 c          304 g          248 t
ORIGIN      
        1 atggcggcga cctgcccccg tccatgcgcc gggtgcctgg ctcggtctgg ctgatcgccg
       61 acagttccag tccagttggc ctttcggtag caaaagacgt aattcagcgc gggcatcggg
      121 tggttctcgc ggcccgcaac atggcagcca tgcaggatct cgtccgagga tttccggata
      181 cgactttcgt ggtcgctctg gatggcacct cgaccgagga catcactcgc gtcgtccaag
      241 aatccaaagg ccggttcggg ccgatcgagg tactggtcaa tacgccaggt gtcggcagtc
      301 ttccgtccct tgggtgaacc tcgaacttca tgagtattcg cggagaggtg ggatcagctg
      361 aggacgatct ccgcggcaag tccccctcct gatcggttct tgaggctgag cgagccacca
      421 atcgccaatg tcagctgttg ggctatggcc aggccgagac cggttccccc tgtatcccgg
      481 ttccgggact gttccagccg atagaagggc tgaagaacag cttcgagttg cccatcggga
      541 atccccggtc cgcgatcgca gacggcaatg catatttttc cgtccttcgt tctgatgatt
      601 tcaacctcag cctctccggc aaacttcagt gcattgtcga tgagatttgt caggatgcgg
      661 cggagcgcat ggggccgtgt tgtcacaaca gcctcaatca ttcttgcggt ttttacagcc
      721 ttcccggtat cctggtaatc gaagacgatg ctctcaacga aagaaccgat atcgatccga
      781 gccggattct cgacgttgcc atgtgcggtc ctggcatagg ccacaccctc gcgcacaagc
      841 ctctcaattt ccgacaagtc gctgatcaac ttttcctttt ccggaccatc ttcagcgacc
      901 tcagcacgga gtttcatgcg cgtaattggc gtctgaaggt catgagagat cgcagccagg
      961 atctggactc gctccttgag gtagtgggtg atccgatcct gcatggtgtt gaaagctgtc
     1021 gcggcatagg cgacttcggt tggtcctgtc tcatcaagcc gagccgc
//
LOCUS       DASTWE010000533         1143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1307242, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000533 DASTWE010000000
VERSION     DASTWE010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307242"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..251)
                     /locus_tag="VFT30_05535"
     CDS             complement(81..251)
                     /locus_tag="VFT30_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092125.1"
                     /translation="MKVEIVEEGDDRFLLQVFADGSEERVAILEEPAKKRRLSAKIAW
                     YWDLKAGRRKFY"
     gene            350..538
                     /locus_tag="VFT30_05540"
     CDS             350..538
                     /locus_tag="VFT30_05540"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5092126.1"
                     /translation="MDKDRVEGSATNIKGKIKEGVGKMTGDAKTEAEGQAEQVKGKAQ
                     NAVGGLKDKAREALNGRD"
     gene            complement(672..>1143)
                     /locus_tag="VFT30_05545"
     CDS             complement(672..>1143)
                     /locus_tag="VFT30_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5092127.1"
                     /translation="ANPAFSEAALKMRAERARTLGVRLLIVNASNPVDIDRAFASLVA
                     ENAGALLVGPDTFFQSQRERIVTLAARNRVPASYPWPEDVSAGGLFSYGADFIDVFRR
                     AGSYVGRVLKGEKAGDLPVQQPTRFKLAVNLKTAKALGLAVPPSILARADEVIE"
BASE COUNT          266 a          357 c          290 g          230 t
ORIGIN      
        1 cgcttcccgc cccttccctt tcggattccg gaccctcatg gctctcgtga ccggaccggg
       61 ttgtcgcctc acctgcagat tcaatagaac ttgcgtcgtc cggccttcaa atcccaatac
      121 caagcgatct tggctgacag ccgcctcttc tttgctggct cttcaaggat cgccacgcgc
      181 tcctccgagc catcagcaaa cacttgcaag agaaagcgat cgtcgccttc ttctacgatc
      241 tcgactttca ccagttttct ccgcattccc ggaaacgtga gaatggaaat acggttccac
      301 gcaaccactc atcccgtcgc tggttgccca gaacccatag gagaacgaca tggacaaaga
      361 ccgcgttgaa ggttccgcca cgaacatcaa aggcaaaatc aaggaaggcg tcggcaagat
      421 gaccggcgat gcgaagacag aagctgaggg tcaagctgag caggtcaagg gcaaggctca
      481 gaacgccgtt ggcggcctga aagacaaggc ccgcgaggca ttgaacgggc gggactagca
      541 caccattact tcgagccccc gctgacgcgg gggcttcctt catcgtgcaa tcgttggctc
      601 catcgtcggc tgctgccgcc ccaacgtccg ctcctgctcc gggggacatt ctaaaaacgc
      661 tctattgacc gctattcaat cacctcgtca gcgcgggcta atatcgaggg cggcaccgcg
      721 aggcccaagg ctttcgcagt tttcagattc acagccaatt tgaatcttgt tggctgctgc
      781 accggaagat cgccggcctt ctcacccttc aatacgcgac caacgtaaga cccggccctg
      841 cgaaacacgt cgatgaaatc agccccatag ctgaacagac cacccgcact aacgtcctct
      901 ggccaagggt aagacgcggg gacgcgattg cgagcagcta gggttacgat ccgttcgcgt
      961 tgactctgaa agaatgtatc tggaccaact agcaacgcgc cggcattttc ggcgacgagt
     1021 gacgcaaatg cacgatctat atcgaccgga ttgcttgcat taacaatcaa caaccgaaca
     1081 cctaacgtgc gtgctctttc tgcccgcatc ttcaatgcag cttcggaaaa tgccgggttg
     1141 gcg
//
LOCUS       DASTWE010000534         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_531793, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000534 DASTWE010000000
VERSION     DASTWE010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531793"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFT30_05550"
     CDS             complement(<1..890)
                     /locus_tag="VFT30_05550"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU5092128.1"
                     /translation="MKSRIGVGIVGFGTVGTGVAKILLDNAALITRRAGVPIELVRVA
                     DLDIVRDRGVPLKPGLLTTDIKHILTDPDIDIVIELIGGYDTAKRVILETIAAGKHVV
                     TANKALLALHGEEIFEAATRKGVDVGFEASVGGGIPVIRALTEGLAGNTIESIYGIIN
                     GTSNYILSRMTHEGHGFEEVLKDAQQAGYAEADPTFDVAGIDSAHKLAILVSLAYGTA
                     VNFKEIYTEGITNITPTDIAYAKQFGCTIKLLGIAKLVEGEIEARVHPTMLPSTSPIA
                     QVEDVYNAIQLVGDAVGDVVL"
     gene            complement(887..>1528)
                     /locus_tag="VFT30_05555"
     CDS             complement(887..>1528)
                     /locus_tag="VFT30_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5092129.1"
                     /translation="NLEFFKKIVAFAKEHNVIVIHDLAYADLVFDGYKAPSFLQIPGA
                     KDCGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKIKSYLDYGIFQPLQIASVIALN
                     GPQDCVKETVLRYQKRRDTLVGGLNRIGWQVAKPLATMFVWARIPLPYRHMGSLEFSK
                     LLLKEAKVAVSPGIGFGEGGDEYVRFGLVENEHRTRQAVRGIRKALKLDGEEE"
BASE COUNT          338 a          450 c          381 g          359 t
ORIGIN      
        1 agcacgacgt caccgacggc atccccgacc agttggatgg cattgtacac atcttcgact
       61 tgggcgatag gtgaggtaga gggtagcatg gttggatgaa cccgcgcctc aatttctccc
      121 tccacgagtt ttgcgatccc cagcaactta atcgtacagc caaactgttt ggcataggca
      181 atgtcggtcg gggtgatatt cgtaatccct tctgtgtaga tttccttaaa gttgaccgcc
      241 gttccgtacg cgagactgac gagaatggcg agtttgtgcg cggaatcgat cccggccaca
      301 tcgaaagtcg ggtccgcctc ggcataaccg gcttgctgag catccttcag cacttcttca
      361 aaaccgtgtc cctcatgggt cattctcgac aggatatagt tggatgtccc attgatgatg
      421 ccgtagatgg attcgatcgt attgccggcc agcccctccg tcaacgcccg aatgacagga
      481 atgccccctc cgacactggc ctcaaatccc acatccacac ctttgcgcgt ggcggcttca
      541 aaaatttcct ctccatggag agccaacagc gctttgttcg ccgtgaccac atgtttcccg
      601 gcagcgatcg tctccaggat gactcgctta gccgtatcat atccaccgat cagttcgatc
      661 acgatatcga tgtctggatc agtcagaatg tgtttgatat cggtggtcaa cagaccgggt
      721 ttcagcggta cccctcgatc tcgcacaatg tccagatccg ccacgcgaac aagctcgatt
      781 ggcacaccgg cgcggcgggt aatgagggcg gcattatcga ggagaatctt ggcaacaccg
      841 gtacctacgg tgccgaaccc aacaattccc actccaatgc gcgacttcat tcttcttccc
      901 catcgagctt gagggcttta cggattcctc tgacggcctg cctggtacgg tgttcatttt
      961 ccaccagtcc aaatcgcacg tattcatcgc cgccttcgcc gaatccaatc ccgggtgaga
     1021 cggcgacctt cgcctctttg agcaggagtt tcgaaaactc caacgagccc atatggcgat
     1081 agggaagggg aatgcgtgcc cacacgaaca tcgtggccaa cggcttcgcg acctgccaac
     1141 cgattcgatt cagccccccc accagcgtat ctctccgttt ctggtagcgc agaaccgtct
     1201 ccttgacaca gtcctgaggg ccgttcaatg cgatcacact ggcgatttga agaggctgga
     1261 atataccata gtcgaggtag ctcttgatct tcgccaatgc tccgacgacc tctcgattgc
     1321 ctacacaaaa gccgactcgc caacccggca tgttgtaggc cttggataat gtatagaact
     1381 ctaccccgca gtctttggct cccgggatct gaagaaagct cggcgccttg taaccgtcga
     1441 acacaaggtc ggcgtaggcg agatcgtgga taacgatgac gttgtgttct ttggcaaacg
     1501 ccacgatttt cttgaagaat tctaaatt
//
LOCUS       DASTWE010000535         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_854850, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000535 DASTWE010000000
VERSION     DASTWE010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854850"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..>1342
                     /locus_tag="VFT30_05560"
     CDS             57..>1342
                     /locus_tag="VFT30_05560"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEU5092130.1"
                     /translation="MPPSSITAKQIGQGTTLISSVTLNLSEPLAVECDLTAIHCSGGA
                     MIATVRAPVMRISGRNIRVTRGVLRIQEAETTKASWAAQGTLSVAGVSPEPGSWWPST
                     SDWAVKFSADQGGIKADLRVDTPTHGEFVTARIEQPLQTAQGVMHGVIGPLTFNGNDR
                     RLSKMIMGLPSSTDLTEGMFTATVDASWSSSFRDRITVMNAVSATTRVVAEQLSGHYD
                     DYVLKGLSTTMVLYTQGLESIVMTQPASIFVAAVQSGVDVANLSASYQIRWKLAEDLP
                     IVDVRDFQCEVFGGTISSPGSVVDLAKPPVTTTFSLRNLDLAKILSVEQQKGLQGTGT
                     LNGILPVTITSGGIIVKDGVIEAQPPGGTIRYASTSESSKNFSESDRQLQLVAQALNN
                     FQYSLLRVGVKYGETGMLDLNARLEGRNPDLRNTPP"
BASE COUNT          312 a          341 c          409 g          280 t
ORIGIN      
        1 tgggaggagt tcaagggacg gtgaagctgg cgcggacaca tgtacgcgga gtgttgttgc
       61 caccatcttc aattacggcg aaacagatcg gacaaggcac cacgcttatt tcgagtgtca
      121 cgctgaacct gtcggaaccg ctggcggtgg aatgcgatct gacggcgatc cattgcagtg
      181 ggggagcgat gatcgcgacg gttcgggctc cggtgatgag aataagcggt cggaacattc
      241 gcgtcacacg aggtgtcttg agaatccagg aggccgaaac aaccaaggcc tcctgggctg
      301 cgcaagggac gttgtcggtc gctggggtga gtccggagcc gggttcttgg tggccttcca
      361 cgagtgattg ggcggtcaag ttctcggctg atcaaggcgg catcaaggcc gaccttcgag
      421 tcgatacccc gacgcacgga gaattcgtga ccgccagaat agaacaaccg ctgcagacgg
      481 cacaaggagt catgcatggg gtcatcggtc cgctgacgtt caacggcaac gaccggcggc
      541 tcagcaagat gatcatgggg cttccttctt caacggatct gaccgagggg atgttcacag
      601 ccaccgtcga tgcgtcctgg tcgagcagct ttcgcgatcg gatcactgtg atgaatgcgg
      661 tatccgcaac cacgagggtt gtggccgagc aactgtccgg tcactacgac gattatgtcc
      721 tgaagggctt gagcactacg atggtgttat acacccaggg gcttgaatca attgtcatga
      781 cccagccggc ctcgatcttc gttgcggcag tgcagagtgg tgtagacgtg gccaatcttt
      841 cggcttccta ccagataaga tggaaattgg cggaagatct gccgatagtc gatgtcagag
      901 acttccagtg cgaagtattt ggaggaacga tcagcagtcc gggttctgtg gtagacttag
      961 ccaagccacc ggttacaacg accttttctc tgcggaatct cgatctcgcg aaaattctca
     1021 gcgtggaaca gcagaaggga cttcagggaa ccggaacact caacgggata cttcccgtta
     1081 caatcacgtc gggtgggatc atcgtgaaag atggagtgat cgaggcacag cctccaggag
     1141 gtaccatacg gtacgcatcg acgtcggaat cgtcaaagaa cttttcagaa tcagatcggc
     1201 aactccagct cgttgcgcaa gcgctcaaca actttcagta ctcactattg cgcgtgggcg
     1261 tgaaatacgg tgaaaccggc atgttggacc tcaatgctcg gttggaaggc cggaatccgg
     1321 atctcaggaa tactcctccg at
//
LOCUS       DASTWE010000536         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1501379, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000536 DASTWE010000000
VERSION     DASTWE010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501379"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1443
                     /locus_tag="VFT30_05565"
     CDS             343..1443
                     /locus_tag="VFT30_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092131.1"
                     /translation="MQEHVIVKGTCFALFAYDIGLSINLTDAERRILAGAERGRLRHR
                     TRAPQYFEYRPAPLRLVQEGSVFDVSQYQASPTVEAMVYDFGAVTITYRFPIEGPFAR
                     LLDLSESLYENERLLAESRTRVDQLLQVLGPAVERPAIAQEVEDYIIFSIESHVPETP
                     PLWTSNENDLAHLLRAERMPFSEQEVADAVACRISFGQEDAAVIDWHSTALFGKEMDD
                     VRAVLEFANVELLEMRILDEQLDRALDEGYEALSRKPRLLSLPGSHEKDLTHIAQLQV
                     DSALIFERVTNTLKLLGDQYLARVYRLVSQRFHLEAWDASILRKLQTLESIYGKMSDR
                     AGTRRMEVLEWIIIILITLSIALPFLPMGVGH"
     gene            1681..>2140
                     /locus_tag="VFT30_05570"
     CDS             1681..>2140
                     /locus_tag="VFT30_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092132.1"
                     /translation="MSVAASQLSGRREIPLGAQETVVSSGVNGINGIVVTSRRLLGFS
                     SRALTWSKKELDVNEKVLERKILPTFSLVRTDRHLYGFRGVNGLWLEEALGVREKVMR
                     FHSNDYGAVFITNERLVGFTPLLGGFTSKPLDVHEWVVGVDNENGLILVST"
BASE COUNT          486 a          554 c          604 g          496 t
ORIGIN      
        1 agtcaagaag ccgcgccggg acaagcgagc ttccaaaatg tctcgaaaat gctcgttacc
       61 cgaatcattg ctgacactct cctcatcgat gatcgacata gaaatctcct ttttatctga
      121 gtccctgcga actgggtgtc ggtgacgccg caatagcagc ccctttgcaa gcgcgatcac
      181 tgttacagag agccgttaca ttccaatcaa cgcttggtaa cgacctcgtt aaaagttatc
      241 gcttcgaaca cgagaagggt gcgccagtct ctcgtaatca gacataccta tcagtgagca
      301 ggcggtgtaa ctgttagaat gatggcgtgt gaggaaattg ccatgcagga acatgtcatt
      361 gtcaaaggga cgtgcttcgc cctgttcgct tatgatatcg ggctgtccat caatctgacg
      421 gatgcggagc ggcgaatcct cgccggagcg gaacgaggcc ggctgcgcca taggacgagg
      481 gcccctcagt attttgagta tcggccagct ccgctacgac tggttcagga agggagcgtc
      541 ttcgatgtca gtcagtatca ggccagcccg actgttgaag cgatggtgta tgatttcggg
      601 gccgtcacca tcacctatcg ttttccgatc gagggaccat tcgcccggtt gttagacctg
      661 agcgagtcgc tttatgagaa tgaacgactt ctggccgagt cgcgcacccg ggtggatcag
      721 ctgctccaag tgctgggtcc cgcggtcgag cggccggcta ttgcgcagga agtggaggac
      781 tacatcatct tctcaatcga gtctcatgta ccggagaccc ccccgttgtg gacgagcaac
      841 gaaaatgact tggctcatct cttgcgggca gagcgaatgc cgttttctga gcaggaagtg
      901 gccgatgcag ttgcttgtcg gatttcgttc gggcaagagg acgctgcggt catcgactgg
      961 cactccactg cgctgttcgg caaggagatg gatgatgtcc gtgcagttct agaattcgcc
     1021 aacgtggagt tgttggagat gcgaatactg gatgaacaac ttgatcgcgc gctcgatgaa
     1081 ggatacgagg cgttgtcccg caagccgcga ttgctgtcac tgcccggttc ccatgaaaag
     1141 gacctgacac acatcgcaca gctgcaggtc gacagcgctt taatcttcga gcgcgtcacc
     1201 aacaccttga agctgttggg cgatcaatac cttgcacgtg tgtatcggct ggtctcgcaa
     1261 cgcttccacc tagaagcatg ggatgcgagc atcctgcgca aattgcaaac gctggaaagt
     1321 atctatggca agatgtccga tcgagccggc accaggagga tggaagtgct tgagtggatt
     1381 atcatcattt tgatcacgct ttccatcgcc ctgccatttc tcccgatggg ggttggtcac
     1441 taaacgttcc gtgccctctc atctggtagg aggactggat gttccgatcg cgcctcagcg
     1501 tcttggagaa gaccgtagaa ccggatacaa aacgccttca tggaatgtaa cggtgactga
     1561 actccattac cccttgatcc atatctctgc aaaacttgcc tttttctgac tttgacgttt
     1621 tcaccagtcg tcgctacggc aaacccgcaa tctgaggtgg ctttatggtt cgaatcaggt
     1681 atgtccgtcg cggccagtca actgagtggg cgaagggaaa taccactcgg cgctcaggag
     1741 acagtagtca gttccggggt gaatggaatt aacggcatcg tggtgacatc tcgtcggctt
     1801 ttagggtttt caagccgtgc tctaacctgg agcaagaaag aactggacgt gaacgaaaag
     1861 gtcctagaac ggaagatcct gccaaccttc agcctcgtca ggacggatcg gcatctgtac
     1921 gggtttcgcg gcgtcaacgg cctgtggctc gaagaagcgt taggcgtgcg ggaaaaagtg
     1981 atgcgtttcc acagtaacga ctatggagca gtcttcatca ccaatgagcg attggtcggc
     2041 ttcacgccac tactcggcgg gttcacatcg aagcccttgg atgtacacga atgggtcgtg
     2101 ggagtggata acgaaaacgg cctcatcctc gtttccacga
//
LOCUS       DASTWE010000537         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1016792, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000537 DASTWE010000000
VERSION     DASTWE010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016792"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="VFT30_05575"
     CDS             complement(<1..2010)
                     /locus_tag="VFT30_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092133.1"
                     /translation="MQFKSAGDGGKILAEVGGNLTSMVNAIENTDAGTWTPLAESLYE
                     ATRYFAQIKPAFNNSDYSYGVTSKDPYYFRSPDWSLNNPGEYVHCCKSFIMVFTDGAS
                     TMDTNVPAVLQDLAHDHHGNHCTGSTPCANHKTDYPDSGTHYLDDVAYYAHTTDLRQA
                     TLPLLGEAGKDLPGFENVTVYTFYAFGQPLGRELLQTAAKTGGFEDSNGNNKPDKVEE
                     YDAVNNYTGLKGADGLPDTYFESADADDLRDRLVAAITSILQRSASGTSISVLATSAT
                     GEGTVYQAYFYTSQVGKDTANVKWVGYTHALFVDAFGNFREDTDQDGALNYKNDLIVR
                     TRYDDDVSSPTYRQVVADKFTDTSPEDGIADSTTPTITGDLRSIEPIWEAGKELALMN
                     WTTRNVLTWVDTDNDGLVDVGEEMPFNTANCATLRDYLRYAGDTCAGGSNATNLIEFI
                     LGREVDGLRPRQLQVPIGSGSYHVWKLGDPIHSTPTVVAAPRSRYDLLYGDTTYTAFY
                     QKYRTRRQVVYVGANDGMLHAFNGGFYHKGDDPTTTSDVEHGWYTKNPNDNSGGANLG
                     AELWSFVPYQLLPHLQWLARNDYTHVYYVDLKPTISEARIFTPDADHPGGWGTILIGG
                     FRMGGSCGSCVAGGALPMKATIGGVERTFYSAYFVLDITNPEVNPK"
BASE COUNT          450 a          568 c          551 g          542 t
ORIGIN      
        1 tttgggattg acctccggat tcgtgatgtc gagcacaaaa tacgcactat agaacgttct
       61 ctcgaccccg ccgatcgtcg ctttcatcgg aagggctccc ccagccacgc agctgccgca
      121 gctgccgccc atgcggaatc ctccgatcaa gatcgtgccc cacccgcctg gatgatccgc
      181 atccggggta aagattctcg cttctgaaat agtcggtttc agatcgacat aatacacgtg
      241 cgtgtagtcg tttctggcca accattgcaa atgtggtaag agttgataag ggacgaacga
      301 ccagagttcg gccccaagat tggcaccgcc tgagttatca tttggattct tggtatacca
      361 tccgtgttcg acatcgctgg tcgtcgtcgg atcgtcccct ttatggtaaa acccgccatt
      421 gaacgcgtgg agcatcccgt cgttggcacc aacgtagacc acctgacgtc tggttcgata
      481 cttttgatag aatgctgtgt aagtagtatc cccatagagg aggtcgtacc gagatctggg
      541 agctgctacg actgtgggag tcgagtggat tgggtcaccc agtttccaca catggtacga
      601 tccactgcca ataggaactt gaagttggcg tgggcgcaat ccatcaactt ctcggccgag
      661 gataaactca ataaggttcg tggcgttgga tccaccagca caggtatctc ccgcatatcg
      721 gaggtaatcg cgtaaggttg cgcagtttgc ggtattaaac ggcatttcct cgccgacatc
      781 caccaggcca tcgttgtcgg tatccaccca cgtgagaacg tttctggtag tccaattcat
      841 caatgcgagt tcctttccag cttcccagat tggctcgatg gacctcagat cacccgtgat
      901 ggttggagta gtactatcgg cgattccatc ttcagggctg gtatccgtaa atttatcggc
      961 gacgacttgg cggtaagttg ggcttgacac gtcatcgtcg tagcgggtcc tgacaatcag
     1021 atcgtttttg taattcaggg cgccatcctg atctgtgtct tcgcgaaagt ttccgaacgc
     1081 atcgacgaag agcgcatggg tgtacccgac ccacttcaca ttggcggtgt ccttaccgac
     1141 ttggctggta tagaaatagg cttgatagac tgtaccttcg cctgtcgccg aagtggccaa
     1201 caccgagatc gaggtgcccg atgcgctccg ttggagaata ctggtaatgg ccgctaccag
     1261 cctatctcga aggtcatcgg catccgctga ttcaaaatac gtatccggga ggccgtcagc
     1321 accttttagg ccggtgtagt tattgacagc atcatattcc tcgactttgt cgggcttatt
     1381 atttccgttg ctgtcctcga accctccagt cttcgccgct gtttgcaaca gttctcgtcc
     1441 aagtggttgt ccaaaagcat agaatgtgta caccgtcacg ttttcaaacc cgggcaggtc
     1501 tttccctgct tcgcccaaca atggcaatgt ggcttggcgc aggtccgtcg tgtgtgcata
     1561 ataagcgacg tcatcgaggt agtgcgtgcc gctgtcagga tagtccgtct tgtgatttgc
     1621 gcagggagtc gaaccggtgc aatggtttcc atgatggtca tgagccaaat cttgcaacac
     1681 ggctggtacg ttcgtgtcca tcgtcgaggc gccgtctgtg aacaccatga tgaaactctt
     1741 gcagcagtgt acgtattcgc ccgggttgtt taggctccaa tctgggcttc taaagtagta
     1801 ggggtccttg ctggtcacgc cgtaggaata atccgagttg ttgaaggcag gcttaatctg
     1861 agcgaaatag cgcgtcgcct cgtacaacga ctcagctaat ggtgtccagg tccccgcatc
     1921 tgtattttcg atggcattga ccatgctcgt caaattgccg ccgacttcgg ccaggatctt
     1981 tccgccgtct ccagcgctct tgaattgcat tagaccgaat cgagctctat ttccgaccgc
     2041 ctgaataaca ccatttggtt ccgcggcaat ctccgaccac aatttatagg aagtgctgag
     2101 gccgccacat c
//
LOCUS       DASTWE010000538         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_434931, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000538 DASTWE010000000
VERSION     DASTWE010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434931"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFT30_05580"
     CDS             <1..183
                     /locus_tag="VFT30_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEU5092134.1"
                     /translation="EIGADFMMKGTIATILDEADGTKAIFYQVDLQMIDLENNAKVWY
                     GQKKIKKVVEKKRTVF"
     gene            196..>1292
                     /locus_tag="VFT30_05585"
     CDS             196..>1292
                     /locus_tag="VFT30_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092135.1"
                     /translation="MLNPILPRFAHGPISLLPFLAVLVLLTGCGPSINRYLLIEQSLI
                     AGDPQRAAAIVEQTEKEYGAKGRLLYQMDQGMVLQLAGQYQQSSAVLEQAEEEVERLY
                     TRTIRSETAAFLTNDNALPYEGDAYEHVMINVIKALNYAVQGQLQEALVEARRIDHRL
                     NVLSDRVKKADRYRNDGFARYLSGILYEATGDLNNAFIAYRNAYEAYGSMQGWSKMSA
                     PPSLRADLLRTAEALNFTTEFAEYRRAFPEVEWQPSSSQEQLAQVVMISYNGRAPRKE
                     DLFLDLPISLDAAQLVLLNRGVFQSPYRRDRVADSVLYGLNGEVVRVALPRLVAQKTR
                     VVSESMTLTDSFGQSVTVRSEPAQNVTALAE"
BASE COUNT          313 a          328 c          364 g          287 t
ORIGIN      
        1 gagattgggg ccgatttcat gatgaagggt acgatcgcca ccattctaga cgaggccgat
       61 ggaaccaaag cgatcttcta ccaagtggat cttcagatga tcgatctgga gaataatgcg
      121 aaagtctggt acgggcagaa gaagatcaag aaggtggttg aaaagaaacg taccgtcttt
      181 taggttccgc ggacgttgtt gaacccaatc ctcccacgct ttgcccacgg gccgatctca
      241 ttgctgccat ttctcgccgt tctcgtactt ttgaccgggt gcggcccttc catcaatcgt
      301 tacctcctca ttgaacaaag cctcattgcc ggtgatccgc aacgggccgc ggcgatcgtc
      361 gagcagaccg agaaagagta tggagcaaag gggcggctcc tgtatcaaat ggatcaggga
      421 atggtgctgc agttagctgg ccaataccag caaagcagtg cggtattgga acaggcggaa
      481 gaggaggtcg agcgactcta tacgaggacc atccgttcag aaaccgccgc atttctcacc
      541 aacgacaatg ccttgccgta cgaaggcgac gcctacgaac acgtcatgat caacgtgatc
      601 aaggctttga actatgcggt tcagggacaa cttcaggaag cattggtgga agcgcgacgt
      661 atcgaccatc gcttgaatgt gctcagtgac agggtgaaaa aagccgatag gtaccggaat
      721 gacgggtttg cgcggtattt gagcggtatt ctgtatgagg cgactggtga tctcaataac
      781 gcatttatcg cctatcgcaa tgcatatgaa gcgtatggat cgatgcaagg gtggtcgaag
      841 atgtccgctc ctccgtccct gcgcgccgat cttttgcgca cagcggaggc gctcaacttt
      901 acaaccgagt ttgccgagta tcgacgggct tttcctgagg ttgaatggca gcccagctca
      961 tcccaagagc agctcgccca agtcgtgatg atcagctata atggccgcgc tccgcgcaaa
     1021 gaggatctgt tcttggactt acctattagt ctggatgccg cacagttagt cttgctgaac
     1081 cgaggagtct tccagtcacc ctatcgacga gatcgcgtcg cggacagcgt tctgtatggg
     1141 ctcaatggag aagtggtccg cgttgcactt ccgcggttag tcgcgcaaaa gacgcgagtg
     1201 gtctctgaaa gtatgacact gaccgattcg ttcggccaat cggtcacggt aagatccgag
     1261 ccggctcaga atgtaaccgc tctcgcagaa aa
//
LOCUS       DASTWE010000539         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1178058, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000539 DASTWE010000000
VERSION     DASTWE010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178058"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFT30_05590"
     CDS             complement(<1..887)
                     /locus_tag="VFT30_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HEU5092136.1"
                     /translation="MAGPTSIQQKKVVGFRSIMRRFFTIPARYSAITLLVGIAGLSIY
                     GCTDAGSIDPGPQLASLSVSVASLQPPFTSETTGYSVDLPSNTPDVTVSATKSDPNDV
                     LSGAVTAPAGQTTGQTTIPSPGPGSTKDISLTVTSPDGRAKIYTITLRAITLGGDNTL
                     RNLTVSPGTLSPSFTAGTQNYTVDVATAVAQVTVSATKSDVNAVISGDVPNEGQTTLK
                     LDGPGTTKLVSIIVTAPNGTSKTYTITVKRAASSSDDSLSDLTVRPGLLDPDFGSGIL
                     TYTVDVANDMDSVIISATKS"
BASE COUNT          248 a          271 c          347 g          279 t
ORIGIN      
        1 gacttcgttg ctgagatgat cacgctgtcc atatcattgg caacgtccac ggtgtaggtc
       61 aagattcccg aaccgaaatc aggatccaag aggcctgggc gcacggtcaa gtcagatagg
      121 ctatcgtcgc tcgaggacgc cgctcgtttg acggtgatgg tatatgtctt cgaggttcca
      181 ttcggtgcag tgacgataat cgatacaagc ttggttgtac ctggcccgtc gagtttgaga
      241 gtcgtttgcc cttcattcgg cacatcaccc gatatcacgg cattgacatc agacttggtt
      301 gccgagacag tcacttgggc gacggcggtg gccacgtcta cagtatagtt ctgagttcct
      361 gcagtaaagg acggagacaa ggtacctggc gatacggtca agttcctgag ggtattgtcc
      421 ccgccaaggg ttatcgccct taaggtaatg gtgtagatct ttgctcttcc atcgggcgaa
      481 gtgacggtca gcgagatatc tttggttgaa cctggtcctg gcgatggaat cgttgtttga
      541 cccgtggtct gccctgccgg agcggtcacg gcgccggaca acacgtcatt tggatcagac
      601 ttcgtggccg agacggttac gtcaggagtg ttgctgggca gatcaacact gtaaccggtc
      661 gtttcgctgg tgaacggtgg ttggagcgat gcgacggaga ctgaaagact ggcgagttga
      721 ggccctggat caatcgaccc tgcgtctgta caaccataga tgctcaggcc ggcgattccg
      781 acgagaagag tgatggccga atatcgtgcg gggatcgtga agaaacgtct catgatggac
      841 ctgaacccaa ctactttctt ttgctggata ctggtgggtc cagccacccg tgtgaaccat
      901 tagggtagat ggagatatta cgagagggtt tccagatcgg tctatcccta cttgagtata
      961 gataagcagg ggtgcgcgca aggttgatcc taactggtcg ggtcacagat cgggcgtgga
     1021 gccgacagta tcacgagtgg tctgagacga attggatctc agttacgaga cagtttttaa
     1081 cgggacggcc ggttcacagt gatcctgtaa gtttttgtgg tcactcgatc ctccgcggtc
     1141 acagc
//
LOCUS       DASTWE010000540         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_208718, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000540 DASTWE010000000
VERSION     DASTWE010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208718"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFT30_05595"
     CDS             <1..976
                     /locus_tag="VFT30_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEU5092137.1"
                     /translation="DRQGFLDDLSTLLNDDRPLVVIGPMLSKNLPVMAEMAQKTRVPL
                     ITPAATLPNVRRLGNYLFSTSLTYAMQAERIATYAAKEQGYRRFCILHPDTIYGRELA
                     RLFAQEVRRLDGEIISIEMFKEGETDFAPQIQRLKAEDLKKYGLAIPVDVPRLPGKPL
                     GRNEKRVLYTPGFDAIFIPSRSHEVGLLAAQLAFHDIKAPLLGTNGWNSQDFARTADR
                     TVDGATFVDGFFIDSPNPSVQEFVQRYHKRFQSTPSLFTMQGYDAARVVIEGIRHGAT
                     SGEALHDFLMTQRNLPTLAGPAGFGPDGTLQRPLFLLQVKQGKFVQLD"
BASE COUNT          267 a          375 c          316 g          257 t
ORIGIN      
        1 cgaccgacag ggctttttgg acgatctctc aaccctactc aatgacgacc gtccgcttgt
       61 cgtcatcggt ccgatgctgt caaaaaatct tccggtcatg gccgaaatgg cgcaaaagac
      121 cagggtcccg ttgattacac cggcggcgac actccccaat gtccgccggt tgggcaacta
      181 tctcttcagc acgtcgttga cgtatgccat gcaggcggag cgcatcgcga cctatgcggc
      241 gaaagaacag ggctatcgac gattctgtat tctgcatcct gacaccatct atgggagaga
      301 acttgcccgc ctcttcgcgc aggaggtgcg ccggcttgat ggtgaaatca tttccatcga
      361 aatgttcaag gaaggggaaa ccgattttgc tccgcagatc caacggctca aggccgaaga
      421 tctgaaaaag tatgggctgg cgattccggt tgacgtgcca cgactgcccg gcaaacccct
      481 cggccgaaac gaaaagcgcg tcctctatac cccaggattt gacgcgatct tcattcccag
      541 tcgctcacac gaggtcggac tccttgccgc acaactggcg tttcatgaca tcaaagcccc
      601 gctgctgggc accaacggct ggaactctca ggactttgcc cgcaccgccg atcggacagt
      661 cgatggcgcc acattcgtgg acggcttctt catcgacagc ccgaatccct ccgtgcagga
      721 gttcgttcaa cggtaccata agcgctttca atcaaccccg tcgctcttca ccatgcaggg
      781 atatgatgca gccagagttg tcattgaagg aatccgccac ggagcaactt ccggcgaagc
      841 gctccacgac ttcctcatga cgcagcgcaa cttgccgaca ctcgccggac ctgccgggtt
      901 cggaccagac ggcactctgc aacgaccgct ctttcttctg caggtgaaac aaggtaagtt
      961 tgtgcagttg gattagcagc atgttgaaaa agtcgtccag tttcgttctc acaagacaca
     1021 gccgtctcac catctcgacg gcgttcacaa acgtgccacg ccttattcga cgcggcgtga
     1081 acctcagagg ctcaacgtac gggccaaaat atgcctcgcc tcttcgctcc tctgcggcct
     1141 tgctcgtgga gaagcgcatc tcggcacgtc ggcgttggac aggtgagatc gtcggctgtt
     1201 ttgaacaccc tgcag
//
LOCUS       DASTWE010000541         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_79450, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000541 DASTWE010000000
VERSION     DASTWE010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79450"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFT30_05600"
     CDS             <1..599
                     /locus_tag="VFT30_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5092138.1"
                     /translation="ESSSGRDGLIEAQARNPDLILLDLGLPDLDGGEVIRQVREWAVT
                     PIIVLSARDQEQAKVAALDLGADDYITKPFGVNELLARVRTALRHVARTDDSGESVFL
                     LDNLKVDLGRRQVFVSGKEVHLTPIEYKLLTTLIRYAGKVLTHRQLLKEVWGPLHVDE
                     GHYLRVYMRQLRNKLERNPAQPRYLVTELGVGYRLRTE"
     gene            696..>1670
                     /locus_tag="VFT30_05605"
     CDS             696..>1670
                     /locus_tag="VFT30_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5092139.1"
                     /translation="MQFLELRPNTMQAIAGNLATVNVEKAHDGPGWRSPIGWLLLVWW
                     LFAPVPVCIAQDLNNQSENDRLPPASSSNPMWFGKTDWHYGAYVDVGYIGNFNFPDNH
                     LWRSRTTAFQHNELSPNMGLAYVRKDASVSSRWGMELGFQGGRDSEEFAFWVGERRVD
                     GADVLRHVHRANVSYLTPVGKGLTFTAGLFNSLMGYESLYARDNANYTRSWIADNTPY
                     MMFGVNAQYPVSDDLTVTAFIVNSYYHLAHPNDLPSYGGRWAWKATPRVTFVQTLYGG
                     PDQTDMSLEFWRLYGNHILEWKGDDVTVAASFDIGTESIAGQPGNPRTF"
BASE COUNT          391 a          416 c          465 g          398 t
ORIGIN      
        1 atgaatcctc gagcggacgg gacgggctga ttgaagccca ggcgagaaat cccgatctta
       61 tcctgttgga ccttggcctg cctgatctcg acggaggcga ggtcatccgc caggtgcggg
      121 aatgggccgt aactcccatt atcgtactgt ccgctcgtga tcaggaacag gcgaaggtgg
      181 cggcactcga tcttggtgcc gacgactata tcaccaagcc gttcggagtg aacgaacttc
      241 tcgcacgggt gcgaactgcg cttcgtcatg tagctcgaac cgatgatagc ggggaatcgg
      301 tgtttttgct cgacaatctg aaggttgatc tcgggcggcg gcaggtgttc gtctccggga
      361 aggaagttca tctcacacca attgaataca agctgctcac cacattgatc cgatatgccg
      421 gcaaagtatt gacccaccgc cagctgttga aagaagtctg ggggccgctt catgtggatg
      481 aaggacacta cctacgggtc tacatgcgcc aattgagaaa caaactggaa cgcaatccgg
      541 cgcagccgcg ttatcttgtg acagagttgg gtgttggata tcgacttcgg acggaatgac
      601 ctcaccgccc ttcttcacgc tattctgata cttacctctc ctgtttttat cccgttttga
      661 tatctgacaa agtatagttt gccggtgagc ttggaatgca gtttcttgaa ctgagaccga
      721 acacgatgca agccattgcc ggaaatttag ccacggtcaa tgtggaaaag gcacatgatg
      781 gtccgggatg gcgcagccct attggttggt tgctgcttgt atggtggctt tttgcgcccg
      841 tgcctgtctg catcgctcaa gatttgaata accagtcgga aaatgacagg ctgccaccag
      901 ccagttcatc caatcccatg tggttcggga agacagactg gcactacgga gcgtatgtag
      961 atgtcggtta catcggcaat ttcaattttc ccgacaacca tctctggcgc agtcgcacca
     1021 cagcattcca acacaacgag ctttcaccca atatgggact ggcatacgtg cgaaaggatg
     1081 cgagtgtctc ctctcgctgg ggtatggaac ttggcttcca gggcggtcga gattcagaag
     1141 agttcgcgtt ttgggtgggt gaaagacgag tcgacggagc agatgtgctg agacacgttc
     1201 accgtgcgaa tgtgtcatat ctgacaccgg tcggaaaagg actgacgttc acggcaggtc
     1261 tgttcaacag cttgatgggg tatgaatcac tctatgcaag agacaacgct aactatactc
     1321 gatcatggat cgcggacaat acgccttaca tgatgtttgg cgtgaatgcg cagtatcctg
     1381 tgagcgacga tctcactgtt accgccttta tcgtgaacag ttattatcac ctagcgcatc
     1441 cgaacgatct cccgagctat ggcggaagat gggcctggaa agctacaccg agggtaacat
     1501 tcgtgcaaac cctctacggt ggaccggatc agacggatat gtccctggaa ttctggcgtc
     1561 tgtacgggaa tcacatcctg gaatggaaag gtgacgatgt gacggtggcg gcatctttcg
     1621 atattggaac cgaaagcatc gctggtcagc ctggcaatcc acgtaccttt
//
LOCUS       DASTWE010000542         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1275152, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000542 DASTWE010000000
VERSION     DASTWE010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275152"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFT30_05610"
     CDS             complement(<1..757)
                     /locus_tag="VFT30_05610"
                     /inference="COORDINATES: protein motif:HMM:NF040095.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092140.1"
                     /translation="MSKSTPAFARAHIAELVFDRVEHEGTIAIFYRIAGQSLLKYLPL
                     SKYERQSQLKTLFAQTNTVLLEEWNGNPTFQQAVPPQNVLHTWLGFRLEAGGNTERFL
                     LETRGVNPDVAGLLINGRVFPNPLFYARKPEAWGTARAIDVAYGFIHGDLNTNNILVK
                     FADDKESLAGYYLIDFALFKEAMPLLYDQRYLEMSYLMHATSQVSFAKSVNFLTLLAV
                     ADTPDPHRVPIEMAGVSEVIGTARAAFAAWVEAN"
BASE COUNT          262 a          301 c          299 g          273 t
ORIGIN      
        1 gatttgcttc cacccacgct gcgaatgcag cgcgagccgt cccgatcact tcactcacac
       61 cagccatctc gatcggcacc ctgtgagggt ccggtgtatc cgccacggct aagagggtga
      121 ggaagttgac agacttcgca aaagagacct gtgacgtggc atgcatcagg tacgacattt
      181 ccaggtagcg ctggtcatac aacagcggca tggcttcttt gaacagcgca aaatcgatca
      241 gataatagcc tgcaagcgat tctttatcgt ccgcaaattt gaccaggatg ttattggtgt
      301 tcaggtcacc gtggatgaat ccgtacgcca catcgatggc gcgcgcggtt ccccatgctt
      361 ccggcttgcg tgcataaaac aatggatttg ggaacacgcg tccattgatg agcaggcccg
      421 cgacatccgg attcaccccg cgtgtctcca gtagaaatcg ttccgtgttg ccgccagcct
      481 ccagccgaaa gccgagccag gtgtgcaata cgttttgtgg aggaacggct tgttggaacg
      541 tgggattgcc attccactcc tccagcagga ccgtattggt ttgtgcgaaa agggttttga
      601 gctggctttg ccgttcgtat ttcgataagg gcagatactt cagtagggac tgaccggcga
      661 tccggtaaaa gatcgcgatg gtcccctcat gttcgacccg gtcaaatacc agttcggcaa
      721 tgtgtgcgcg ggcaaacgca ggcgtggact tgctcatcac cgtgttatgc cgcgtgactt
      781 catcggacct ggcgtttttg cctttgcgat ccagtttgag aatgcaatgc tcgacgcgcc
      841 cgttttgcct cgaagaaacg ctgaccagat agacaactgc acctgaccat cccccaacca
      901 ataactgcag tggcgcgacg gtgatcttgt atcgatcctg tgccagccga atgacatgct
      961 gctattcttc cggcagggag tggaaatcgg ttgtcaattc gggcattgcg ccagcctttt
     1021 catttaaaaa attgcgatcg tcaaaatgag ggtgaatcga tcacaaaaag aaggtgatca
     1081 taagtttcat cactgaatca ttgaaccctt cccttaggta gtatacctag acttc
//
LOCUS       DASTWE010000543         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_596308, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000543 DASTWE010000000
VERSION     DASTWE010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596308"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1190)
                     /locus_tag="VFT30_05615"
     CDS             complement(<1..>1190)
                     /locus_tag="VFT30_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092141.1"
                     /translation="LSQQPNAIINGKADDNGSDHTKPCKDKKEKKELHDKNSKEDDCL
                     PGGSSNGISKGDFNGDGIADLAVGVPDEDVNGFRDAGAINVIYGTVDGLHQNFVQLNQ
                     FWTESNVVPLDPGITAGTNHHFGSALAAGNFNGDQFSDLAIGVPFHDLVVPGPDLDDA
                     GLVIVLYGSANGLTPIESLTTTGSISISQSSPLILDEAETGDRFGSALSWGDFDGDGF
                     GDLAVGVPDEDDEDPVAGTTDVDAGAVQILYGSSTGLTAARNQIFTQRSPGILDVTEP
                     GDKFGSVLAAGDFNGDGQSDLAIGIPLEDVGDLPNAGAVSIIYGSTQGEITVNGGLTS
                     TDDQFWTQDSVQGFSEIQEQADALDRFGASLAVGDFDGDLRRSAPATSKTSDLAIGVP
                     FEDV"
BASE COUNT          285 a          336 c          264 g          305 t
ORIGIN      
        1 gacatcttcg aaaggtacac cgatcgccaa gtcactggtt ttgctagtgg cgggagcaga
       61 acgcctgagg tctccgtcga aatccccaac ggcaaggctt gcaccaaacc tatcgagcgc
      121 atctgcctgt tcttgaattt ctgagaaacc ttgcactgaa tcctgcgtcc agaattgatc
      181 gtccgtgctg gtcaagcctc cgttgacagt tatttcgccc tgtgttgagc cataaatgat
      241 actaaccgca ccggcattcg gcaggtctcc cacatcttcc agggggattc caatggctag
      301 atcactctgg ccatcgccat tgaagtctcc agctgccagc acgctgccga acttatcacc
      361 cggttcggtt acatcaagga tgccggggct tctttgtgta aagatctgat ttcttgcagc
      421 agtcaatcca gtactcgaac cgtataaaat ctgaacggca ccggcatcaa catcagttgt
      481 tccagctacc ggatcttcat cgtcctcatc aggtacgccg actgctaaat caccaaaacc
      541 atcgccatca aagtcacccc atgataaggc gctcccgaat cgatcacctg tttcggcttc
      601 atccaaaatg aggggcgaag attgcgagat agaaatgctt ccagtcgtag tcaggctttc
      661 tatcggagtc aggccgttcg cggaaccgta gaggacaatt accaatcctg catcgtcaag
      721 atcaggtccg ggcactacca aatcgtgaaa cggtactcca atcgccaggt cagaaaactg
      781 atcgccgttg aagttgccgg ccgccagggc tgaaccaaaa tgatggttcg ttcccgccgt
      841 tatcccaggg tctaacggaa caacattact ctccgtccaa aactgattaa gctgaacgaa
      901 attttggtgg agtccatcaa cagttccgta aatgacattt attgctccgg catcccgaaa
      961 cccatttaca tcttcatcag gaaccccaac cgcaagatcg gcaatgccat ctccgttgaa
     1021 atcacctttg ctgattccgt tgctgctccc tcccggcaga cagtcatctt cctttgagtt
     1081 tttatcgtgg agctctttct tttctttctt atctttgcag ggcttggtgt gatcagagcc
     1141 gttatcatca gctttcccat ttatgatcgc atttggctgt tgcgacagag
//
LOCUS       DASTWE010000544         1323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_208799, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000544 DASTWE010000000
VERSION     DASTWE010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208799"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..861
                     /locus_tag="VFT30_05620"
     CDS             253..861
                     /locus_tag="VFT30_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092142.1"
                     /translation="MQLTDDETVLLADAQFFRKKAAITTKIRTMLEATHGALQHELAG
                     LSLVVPPDFDPNLHQLVKGEHLEDFPYQYLDCPKHFSGANKFTFRTLVWWGHHVTCAL
                     LLEGSEMGRYKRQLLGRFHQLAGRDLELSLAPSLWEWKCGEGYTLPITHDRKAQIAAV
                     VAERSFLKVVRCVSLGYPRVRDGQLAQMVCETFRSMHPLVTP"
     gene            944..>1323
                     /locus_tag="VFT30_05625"
     CDS             944..>1323
                     /locus_tag="VFT30_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092143.1"
                     /translation="MKQSSLRVLGCAIMMATMLAGCRGTGEMRYLDLREKPPMVQVTD
                     IDPIKIAIESFDDRRADKSRVGTRTHLWGGTTHFDVSGDRLGGLITQRLADRLRTRGW
                     RDRVWNVRVASAGSAADADIVISG"
BASE COUNT          280 a          356 c          379 g          308 t
ORIGIN      
        1 aggtttttct caaggcctgt gtctcgaccc atcaggccgt gctgacactc cgtgagatgg
       61 atgtatttca ggatctggtg cggctgcgct gcgctgactg ccggcgactg tacgaactga
      121 ccgtcgagtc gttcgaaaca cgctacaaat agcttgagga tgctgcaaac gccctcccgc
      181 ctcgttcctc aactttgaac tgagggcaaa aataagcatt ccgtcacgtc ccatgtcatg
      241 atacctcatc ctatgcagct taccgatgac gaaaccgtgc tgttggccga cgcacagttc
      301 tttcggaaaa aagctgcgat cacgaccaaa ataagaacca tgcttgaggc gacgcatggc
      361 gcattgcaac atgagctggc tggtctgtcg ctcgtcgtcc ctcccgattt cgatcccaac
      421 ctccatcagc tggtcaaagg cgagcatctg gaggatttcc cgtatcaata tcttgattgt
      481 cccaaacact tttccggcgc gaacaagttc acgttccgca cgctggtctg gtggggacat
      541 catgtgacct gcgcgctttt gctggaaggt tcggagatgg gacgatacaa gcgccagctt
      601 ctcggccggt ttcaccagct cgctggaaga gatctagaac tatctctggc accgtcgcta
      661 tgggaatgga aatgtgggga gggctacacg ctgccgatca cccatgaccg caaggcccag
      721 atcgctgctg tggtggctga acggtcattc ctgaaggtcg ttcgctgtgt gtctttagga
      781 tacccacgag tacgggatgg acagttggcc cagatggtat gcgaaacctt tcggtcaatg
      841 caccccctcg tcacacccta acttttttat cctaattgtc tctgacgcca aacttgggta
      901 gactgggccg ccactctttt tgaaccttga cagggaggag cctgtgaagc aatcgtcatt
      961 acgagttctg ggctgtgcca tcatgatggc gaccatgctg gccggctgta gaggaacagg
     1021 ggagatgcgt tatctggatt tgcgtgagaa gccgccgatg gtgcaggtga ccgacatcga
     1081 tccgatcaag atcgctatcg aatcgtttga cgatcgacgg gcggacaaga gccgtgtggg
     1141 gacccgtact catctctggg gaggaaccac gcatttcgat gtgtcgggtg atcggcttgg
     1201 gggcttgatc actcagcgac tcgccgatcg cttaagaact cgtgggtggc gagatcgggt
     1261 atggaacgtg cgcgtggcct cggccggatc cgccgcggat gccgatattg tcatcagcgg
     1321 aca
//
LOCUS       DASTWE010000545         1449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_984496, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000545 DASTWE010000000
VERSION     DASTWE010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984496"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VFT30_05630"
     CDS             <1..299
                     /locus_tag="VFT30_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5092144.1"
                     /translation="IGDSPIMLVDEFPEHNSFGPKSLKGSPVTIHLYVQNVESVFNQA
                     VAAGAKIMMPVADMFWGDRYGLLEDPFGHHWSIATHVRDVNPEELKEEARKACG"
     gene            350..949
                     /locus_tag="VFT30_05635"
     CDS             350..949
                     /locus_tag="VFT30_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEU5092145.1"
                     /translation="MRFISLTITTLCVTLMVSPILAKEKKHEKHLDEKAMMEMWQKLA
                     QPGEPHKQLESLTGSWTTQTKEWMEPGKPPIESSGTVEMKSVLGGRFLQQEFTGNMMG
                     QPYSGIGTTGYDNLRKKYVSTWIDSMGTGIFFMEGTASPDGKTITLKGGHDEPGGGHM
                     THRAIWKLVDSNTQIFEMYGAHKGEKEMKGMEITYTRKQ"
     gene            997..>1449
                     /locus_tag="VFT30_05640"
     CDS             997..>1449
                     /locus_tag="VFT30_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5092146.1"
                     /translation="MPTIQRITPCLWLDDQAEEAAKFYVSTFKNSRIGPITRYGEAGA
                     QVSGRPKGSVMTVTFEIENQEFVALNGGPLFKFTEAVSFMVKCETQAEINKIWDKLCQ
                     GGKPGQCGWLKDKYGLSWQIVSPEWDKLLRDKDTERSERVMEAILQMTK"
BASE COUNT          408 a          352 c          398 g          291 t
ORIGIN      
        1 gaatcggtga ctctcccatc atgctcgtcg acgaatttcc tgagcataac tcatttgggc
       61 cgaagtcgct caaaggttcg cccgtcacca tccatctcta tgtccaaaat gtggagtctg
      121 tcttcaatca agccgtggca gccggtgcga aaatcatgat gccggtcgcc gatatgttct
      181 ggggagatcg ttacggcctg ctcgaagacc cctttggaca ccactggtcg atcgccacgc
      241 acgttcgaga cgtcaacccg gaagaattga aggaggaggc acgaaaggcc tgcggctgac
      301 cagttcgact ttcatcagaa tgcaccattc atcaacgaag gaggtcacca tgcgcttcat
      361 ttctcttacc attactacat tgtgtgtcac gttgatggtt tctccaatct tggccaaaga
      421 aaaaaagcac gaaaaacact tggatgagaa agccatgatg gagatgtggc aaaaactggc
      481 ccagccgggc gagccgcaca agcaattgga aagtctgaca ggcagttgga cgacccagac
      541 taaagaatgg atggagcctg gcaaacctcc catagaatcg agcggcaccg ttgaaatgaa
      601 gtcggtgctg ggcgggcggt ttctccaaca agaattcacc ggcaacatga tgggccagcc
      661 ttattcgggc atcgggacga ccggctatga caatttgcgc aagaagtatg tgtcgacttg
      721 gatcgactcg atgggcacgg gaattttctt catggaaggg acggccagtc cagacggcaa
      781 gacgatcacg ctcaagggag gccatgacga accaggcgga ggacatatga cccatcgtgc
      841 gatctggaaa ctcgtcgaca gcaataccca gatcttcgag atgtatggag cccataaggg
      901 cgagaaggaa atgaagggaa tggagattac ctataccagg aaacagtaat cgcatccgat
      961 tcatcgatcc atttggaaag agaaaagaga gatacaatgc ctaccataca acgaattacc
     1021 ccttgtctgt ggctcgatga tcaagccgaa gaggcggcga agttctacgt gtcgactttc
     1081 aagaactcga ggatagggcc cataacacgc tatggagaag ccggcgctca ggtctccgga
     1141 cggccgaagg gatcggtgat gaccgtcacc ttcgagattg aaaaccaaga atttgtggcc
     1201 ttgaacggcg gccctctctt caaattcacc gaggcagtct cttttatggt gaagtgcgag
     1261 acgcaagcag aaatcaacaa gatatgggac aaactctgcc aaggcgggaa gccgggacaa
     1321 tgtggatggc tgaaggacaa atatggccta tcatggcaaa tcgtctctcc ggaatgggac
     1381 aagttgctgc gagacaaaga taccgagaga tcggaacggg tcatggaggc cattcttcaa
     1441 atgactaag
//
LOCUS       DASTWE010000546         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_855069, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000546 DASTWE010000000
VERSION     DASTWE010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855069"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VFT30_05645"
     CDS             complement(<1..1063)
                     /locus_tag="VFT30_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5092147.1"
                     /translation="MHPLLARQLKRLGLDGAQPPSSSAVWGELLERIGQSYVEADQGH
                     VLLERALALSSEEMQNLYAQLKTASDTQLAQERNKIQAVLHSMGDGLCVVDSEWKIRM
                     INNQAAFLFGKEAQALEARPVYHMISPGPEEYREECLIIDATFPPLESGEPYRTDDGL
                     LVTADGELVPISLVVTPMLSDGGVIGAVLVFRDISRQKQVESERQQTERLLRRIQAGL
                     SELAKSPRIYGVNLQEAFQTVTRVAAECLHTERASIWFFTADRSAIHCVDLYQLTTQD
                     HSQGLKLSATAYPLYFQELDTERVVAADDAQRDPRTAEFTEGYLAPLGITSMLDVPIL
                     SEGKMIGVICHEHIGTMRHW"
     gene            complement(1070..>1963)
                     /locus_tag="VFT30_05650"
     CDS             complement(1070..>1963)
                     /locus_tag="VFT30_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEU5092148.1"
                     /translation="HTDLRIASAPAQSADDSFAAGRHIAQQLSDSGLKGILVFSDGLR
                     VNGSELVRGLNSEVSTSVVVTGGLAGDGDRFHRTWVLQDRRPQTGFVTAVGFYGDRIR
                     IGHGTKGGWDRFGPERRVTRSKKNILFELDGRPALQLYKEYLGDRASGLPATGLLFPL
                     ALRTNDTDTKSLVRTILAVNEPEQSLTFAGDIPEGSLAQLMKANFDRLIQGASEAASF
                     TKRSAGGCACTLAIAISCVGRRLVLGSRTEEEIEATLEVLPKGTQQIGFYSYGEISPY
                     TAGTCDLHNQTMTLTTLSEAA"
BASE COUNT          429 a          574 c          519 g          441 t
ORIGIN      
        1 tccaatgccg catggtgccg atatgctcgt ggcagatcac cccaatcatc ttgccttccg
       61 atagaatcgg aacgtcgagc atggaggtga tcccaagtgg agccaaatag ccttcggtaa
      121 attcggcggt cctcggatcc cgttgcgcat catcggcggc tacgacccgc tcagtatcca
      181 attcctgaaa atacagtgga taagcggtcg cggacagttt gaggccttga gaatggtctt
      241 gagtggtcag ttgatagaga tcgacacagt gtatggcaga acgatccgcc gtaaaaaacc
      301 agatggaggc tcgctcggtg tgtagacact cagcggccac gcgggtgact gtttgaaatg
      361 cttcttggag attaactcca tagatcctgg gactcttcgc caattcggac aatcccgctt
      421 ggattcgccg aagaaggcgt tcggtttgtt gccgttcgga ctccacctgc ttctgacggc
      481 tgatatctct gaatacgaga accgctccga tgactccgcc gtcggaaagc atgggagtca
      541 ccaccagaga gatcggtaca agctcgccgt ctgccgtcac cagtaagccg tcgtccgtgc
      601 ggtatggctc cccggattca agcggcggaa acgtcgcatc gatgatcaaa cattcttctc
      661 ggtactcctc cggtcccgga gaaatcatgt gatatacggg gcgcgcttcg agcgcttgag
      721 cctcctttcc gaataagaac gcggcctgat tgttgatcat cctgatcttc cactccgaat
      781 ccacgacaca gagtccgtct cccatagaat gcaacaccgc ttggatcttg ttgcgttcct
      841 gtgccagttg cgtgtcgctg gctgtcttga gttgggcata gagattctgc atttcctcgg
      901 aagacagagc gagcgcccgc tccaagagca catggccctg atcggcttcc acatagctct
      961 gtccgatcct ctcaagcagc tcaccccaca ctgccgacga cgagggaggc tgcgcgccgt
     1021 cgagacccaa acgtttcagt tgtcgagcca gtaagggatg catggcggat cacgccgcct
     1081 cgcttaacgt cgtcagtgtc atcgtttgat tatgaaggtc acacgttcca gccgtataag
     1141 gcgaaatctc gccatatgag tagaacccga tctgttgtgt ccctttagga agcacctcca
     1201 gcgttgcctc gatttcctct tcggtccgac tgcccaggac gagacgacgc cccacacaac
     1261 tgatcgcaat ggccagggtg cacgcgcacc cacccgccga ccgtttcgtg aatgatgccg
     1321 cttctgacgc accctggatt agccggtcga aattcgcctt catgagctgc gccagtgatc
     1381 cttccggaat atcgccggcg aaggtgaggg attgttcagg ttcgttcacg gccaaaatgg
     1441 ttcggacgag actcttggta tctgtatcgt tggtgcgcag agcaagtgga aataagagcc
     1501 ctgtagctgg taaaccggac gctcgatcgc cgagatattc cttgtagagc tgaagtgcgg
     1561 ggcgaccatc gagctcaaag agtatgttct ttttcgatct ggtgacacga cgctccggac
     1621 cgaaacgatc ccatcctcct tttgttccat gtccgatccg gatccgatca ccgtaaaaac
     1681 caacagccgt tacaaaaccc gtttgcggcc gtcggtcttg tagcacccag gtacgatgaa
     1741 atcggtcacc gtcgcccgcc aatccgccgg ttaccacaac tgaagtcgag acctccgagt
     1801 tcaaccctcg taccagttcg ctcccgttca cgcgaaggcc gtctgaaaag accagaatgc
     1861 ctttcaatcc tgaatcacta agctgctgcg caatgtgacg tcccgccgcg aatgagtcat
     1921 ccgccgactg ggctggagca ctcgcgatcc tgagatccgt atg
//
LOCUS       DASTWE010000547         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_952023, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000547 DASTWE010000000
VERSION     DASTWE010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952023"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2771)
                     /locus_tag="VFT30_05655"
     CDS             complement(<1..2771)
                     /locus_tag="VFT30_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5092149.1"
                     /translation="MRKDGSIFFQQVLMVRSKPFAGGVLGHHCFIRDITERKQAEKAL
                     ADLNATLEEQVCDRTQALRQSEERFRQFFDNAPNVTALKDHNGRYVYTNRQFDKVFRL
                     SPGIAIGKTDEELFSPEQAAQFQLHDQQVLLNGRGEEFEQVTQQEDGSHTSIVVRFPV
                     TDTSGHLSALGIIATDITERKRTQEQLRLYQEIFTHSIDGIAVIDPQGWYVMQNRAHE
                     RILGYTTDELKGKTPAVHLGEESFAHIAGALAATGSYRGELESRTSGGAAVALDLTAF
                     AVKDAGGRPVCYVSIMRDVTERNRAQAALRESEARFQQFAESVGSAFWIADLVPNGET
                     VVYANSAFTRIWGIPREEWYESVSLWFDSIHPDDRTRVEVSRTRFISGGLKAEFDCEY
                     RIVDRDGRIRWISDRRVKMAGWGHRVAGIAEDITDHRQQLALMAQTEAFGKIGGWEID
                     FLTNHLWWSDETYRLHDTTPELYSPTVDTAVNFYTPESRPIITEALQKGVGQGKAWDL
                     ELELITAKGCRIAVRIAGKVDVVNGRAVRAYGTFQDITERKRAEEALRKAHAELERKV
                     IERTAELQASEERYARATAIGKVGVWELDVLTGQYHGDANLKALFGYAPAELSTDPYA
                     WLNLVHPEDRPIAMKNWELVQSGVADGSHYELRHIKKDGSIVWGDVRGRSVRDQDGRL
                     TNLIGATVDITDRKQAEQALGKSRQAIRALHDISSAQGQSFKERVEALLQLGCRFFDL
                     PIGMETFVRGDELEVGQVNAPGMAFHPGMRVSLSRAYCSEPLRQNGAVCFEHAGASPE
                     WQQHPAYDALKLESYIGTAINGLERTYGTLCFAGTEPRNKPFTDSERDFIQLMARWLG
                     GEMDRQSALDALRQSEERYRTLYDETPSMYFTVDEKGVIRSVNQYGAQYLGYLVEGLV
                     GKPVAAIFF"
BASE COUNT          595 a          866 c          710 g          682 t
ORIGIN      
        1 tcgaagaaga tcgccgccac cggcttaccg acaagaccct caacgagata ccccaagtat
       61 tgcgcaccgt actggttgac ggacctaatg actccctttt cgtctacggt gaaatacatg
      121 gacggagttt catcatagag tgtccggtat cgttcctcgc tctgacgtaa ggcgtcgaga
      181 gccgattggc ggtccatttc accgccaagc caccgcgcca tgagttgaat gaaatccctt
      241 tcggaatcgg taaatggttt attgcgtggt tcggtaccag caaaacacag ggtcccgtag
      301 gtgcgctcca gcccattaat cgcagtcccg atgtacgatt ccaacttcag agcatcatag
      361 gctgggtgct gctgccattc cggagaagca cccgcgtgct cgaaacatac cgccccgttc
      421 tggcgaaggg gctcgctgca gtacgcccta ctcagagaca cacgcatgcc tggatggaac
      481 gccatgccgg gtgcattcac ttgccccacc tccaactcgt cgccacggac aaaggtctcc
      541 atcccgatcg gcaggtcaaa gaagcgacac ccgagctgaa gcagcgcttc gacccgctcc
      601 ttgaacgatt gtccctgcgc cgacgaaatg tcgtggagcg cgcggattgc ctgtcggctc
      661 tttccgaggg cctgttccgc ctgcttgcga tcagtgatat cgacggttgc gccgatcaaa
      721 ttggtcaacc gtccgtcctg atcgcgaaca ctacgacctc gcacgtcacc ccaaacaata
      781 gagccgtctt tctttatgtg acgtagttcg tagtgagagc catcggcaac tccactttgc
      841 accagttccc agttcttcat cgcgatgggc cgatcctctg gatggaccag attcaaccag
      901 gcatagggat cggtcgaaag ttctgccgga gcatagccaa agagagcttt cagattcgca
      961 tctccgtggt attgacctgt taagacatcc agttcccaga ctccaacctt tccaatagct
     1021 gtggcacgcg cataccgctc ttcactggcc tgcaactcag ctgtgcgctc aatgactttg
     1081 cgttccaatt cagcatgggc ctttcgaaga gcctcttcgg cccgtttgcg ctcggtgatg
     1141 tcttggaacg tgccgtacgc gcgcacggcc cgtccgttca cgacatcgac tttgccagcg
     1201 atccgtacgg caatccgaca ccccttcgcc gtgatcagtt ctaactcgag atcccacgct
     1261 ttaccctgtc ccaccccttt ttgaagagct tcggtaataa tggggcggct ctccggtgta
     1321 taaaagttca ccgccgtgtc caccgtcggg ctgtacagct ccggcgtcgt atcgtgcaat
     1381 cggtatgttt cgtcactcca ccaaagatgg ttggtcagaa aatcgatctc ccatcccccg
     1441 atttttccga aggcttccgt ttgggccatc aaggcaagct gttgtcgatg gtcggttata
     1501 tcctccgcaa tgccggcgac acgatggccc cacccggcca ttttgactct tcggtcagaa
     1561 atccaacgga tacgtccatc tcgatccaca atccggtact cgcaatcaaa ctcagccttc
     1621 aaccctccag aaataaatcg tgtacggctg acttccactc tcgtccggtc gtcaggatgg
     1681 atcgaatcga accatagtga cacgctttca taccattctt cacgcgggat tccccagatt
     1741 cgggtaaacg cggaattggc atacacgacc gtctcaccgt tcggtactag atccgctatc
     1801 cagaatgccg aaccgactga ctccgcgaat tgttggaagc gggcctcgct ttcgcgcaac
     1861 gccgcttgcg cccgattacg ttccgtgaca tcgcgcataa tactgacata acagacaggt
     1921 cggcccccgg catccttcac ggcaaatgct gtgagatcca gcgcaacagc cgctccgccg
     1981 ctggtgcggc tttccagctc tcctcgatag ctgccggtcg cagccagtgc tcctgcaata
     2041 tgtgcgaatg attcttcgcc taaatggacc gccggagtct tgcccttgag ttcgtccgtg
     2101 gtgtaaccga gtatccgttc gtgggctctg ttttgcatga cataccagcc ctgaggatcg
     2161 atgaccgcga tgccatcaat ggagtgagta aagatctcct gatacagcct cagctgctcc
     2221 tgcgttcgct ttcgctccgt aatatcggtg gcaatgatgc ccagagcaga gagatggcct
     2281 gaagtgtcag ttacggggaa tctcaccacg atactggtat gggacccatc ctcctgttgc
     2341 gtaacttgct cgaattcctc tccccggccg ttgagtaaca cctgttggtc gtgtaattga
     2401 aactgggccg cctgttcagg agagaacaat tcctcatccg ttttccctat ggctatgccc
     2461 ggagaaagtc tgaatacctt gtcgaactgt cgattggtat acacataacg cccgttgtgg
     2521 tcttttagag cggtcacatt cggcgcattg tcgaagaatt gtcgaaagcg ctcctcgctc
     2581 tggcgcagtg cttgggtccg gtcgcatacc tgctcttcca acgtggcgtt aagatcggcc
     2641 aacgctttct ccgcctgctt acgttcggtg atgtcgcgta tgaaacagtg gtggccaagg
     2701 acaccgccgg cgaacggttt gcttcttacc atcaggacct gctgaaagaa gatggagccg
     2761 tcttttctca cagcccgaca ctcaaactct gcttttccct tttccagtaa ctgctcatac
     2821 gcttgttggg cgatgcacag gtcctctgaa tgc
//
LOCUS       DASTWE010000548         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_273091, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000548 DASTWE010000000
VERSION     DASTWE010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273091"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFT30_05660"
     CDS             <1..198
                     /locus_tag="VFT30_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092150.1"
                     /translation="EAVFREVHRSNLSKVGGYKRADGKWVKPPTYSPADIEPIVEVQR
                     EQPLAERQSVHDATAGVFRSS"
     gene            261..707
                     /locus_tag="VFT30_05665"
     CDS             261..707
                     /locus_tag="VFT30_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5092151.1"
                     /translation="MGRLLSRFRILSFRCQLCTRRFRAYRNQLPGQTSITDRRQYKRL
                     PVSFRANLLAENAVRMDNRVTDISMGGCTLETTTTLPQGTFIELVIKPASDEEAIKIE
                     TAMVCSSRSESMGIRFLEVVADDKNRLSQVILSLLVGQSLHSNLLS"
     gene            complement(723..>1042)
                     /locus_tag="VFT30_05670"
     CDS             complement(723..>1042)
                     /locus_tag="VFT30_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5092152.1"
                     /translation="LAMSSRNVYLSPDERKRAVTLYKSLQAGAEAIRRGVTDGATIQE
                     AMARIIKGEPAMTVDYLAVCDPHRLEPLSAVTSRVVLLGAVRIGSVRLIDNLLVIPPR
                     RSS"
BASE COUNT          231 a          286 c          298 g          227 t
ORIGIN      
        1 gaagccgtgt ttcgggaggt gcaccggtca aatctgagca aagttggcgg ctacaagcga
       61 gccgatggga aatgggtgaa gccgcccaca tattcgccag ccgatatcga accaattgtg
      121 gaggtacagc gggagcaacc gttggcggag cggcaatccg tgcatgatgc caccgccggt
      181 gtgttccgga gttcatgaga gatttacact gcccaagttg cggaacacct ttcgttcgag
      241 tcgtctatga cgagggaacg gtggggcgac ttttgagtcg cttcaggata ttgtcattcc
      301 ggtgccaact ttgcaccagg cgtttccggg cctatcgaaa ccagctccca ggccagacct
      361 cgatcacgga tcgccggcaa tacaagcgat tgccagtatc gtttcgggcc aaccttctcg
      421 cagagaacgc cgtacgaatg gacaatcgtg taacggatat ctccatggga ggctgcacgc
      481 tggagacgac gacaaccttg cctcagggga catttatcga gctcgtcatc aaaccggctt
      541 ccgacgaaga agccattaag attgagacag ccatggtgtg ttcttcgcgt tctgaatcga
      601 tggggattcg gtttctcgaa gtggtggcag atgataagaa ccgtctcagc caggtgatcc
      661 tcagcttgct ggtgggacaa agcctccact cgaatctcct ctcttaagga cacctcatcc
      721 cattaggaag accgacgtgg agggatgacc agaaggttgt cgataagccg aactgaccct
      781 atccgcactg caccaagaag cacgactcgt gatgtcacgg cggaaagcgg ttccaaccta
      841 tgtggatcgc agaccgccag gtagtcgacg gtcatggctg gttcgccttt gatgatccgg
      901 gccatcgcct cctgaattgt cgctccatca gttacgcctc ttcgaatcgc ctcggctcct
      961 gcttgcaggc tcttgtacag cgtgacggcg cgctttcgtt catccggcga caggtagaca
     1021 ttgcgcgagc tcatcgcgag tc
//
LOCUS       DASTWE010000549         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_661242, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000549 DASTWE010000000
VERSION     DASTWE010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661242"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFT30_05675"
     CDS             complement(<1..796)
                     /locus_tag="VFT30_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092153.1"
                     /translation="MTTDLKTAQEMMAVAVAARAAEDPEIVSVKRVLKLLDKTAKSNR
                     TYGSTNPVALKFTQQLFEELTNHLAAYSKLAFLVQRSELRCKDHVVYQAEKDGGSESI
                     AFKLYADGIRELVLHQGLTQEDLSLFLASLWSDADSNEDDDDIVTRLWSRNLSTITLV
                     TAEELSKSSDGNDGFTRLNGSMSSSDSTLRELLDRERERKKRGEVGTDHEGSGSGKAK
                     SRFQSGLAGYVITEEELAALTQEIEAEQKQDSLMRILDILTAILASE"
     gene            905..1543
                     /locus_tag="VFT30_05680"
     CDS             905..1543
                     /locus_tag="VFT30_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU5092154.1"
                     /translation="MSRTPLVICFGDSLTVGFQSPAKDNPVGQETPYGQFLQSFLGGA
                     GQVRISGICGELTGEMVTRFKRDVLDHQPGYVPILGGTNDLGWNVPLSEIIGNLATMY
                     ERTLAMGSLPIPVTVPSIRVEDASGSREGLEWIADHLARRSRLNQLIHDYADSKGLAY
                     VDLFAATVDSKSGQLEELYSNDGIHLTTAGYRLFAEHVARILKPLLGQDLQP"
BASE COUNT          377 a          449 c          474 g          419 t
ORIGIN      
        1 tctctgacgc taggatcgcg gtcagaatgt ccagaatgcg catgagacta tcttgttttt
       61 gctctgcctc aatttcttgc gtcagggcgg cgagttcttc ttccgtgatc acataaccgg
      121 caagtccaga ttggaaacgg cttttggcct ttccagaacc gcttccttcg tggtcggttc
      181 ccacctcgcc tcgctttttg cgttcgcgct cccgatccag caattctctg agcgtcgaat
      241 ccgatgaact catacttccg ttgagacgag taaacccgtc gttaccgtcc gaagatttcg
      301 agagttcttc ggccgtcacg agcgtgatcg tcgacagatt ccgagaccaa aggcgcgtga
      361 cgatatcatc gtcgtcctca ttcgaatcgg cgtcgctcca taaggaggcg aggaaaagcg
      421 acagatcctc ttgtgtgaga ccttggtgaa gaacgagttc tcgaatgccg tcggcgtaca
      481 atttgaaagc tatgctttcg ctcccaccgt ctttctccgc ctggtacacg acgtggtcct
      541 tgcaccgcaa ttcggaccgt tggacgagga aagccaattt ggaatacgca gcgaggtgat
      601 tggtcaattc ctcgaacaac tgctgcgtaa atttcagggc gacaggattc gttgagccgt
      661 acgtccggtt cgatttggcc gtcttatcca gtagttttag aacacgtttg acggagacaa
      721 tctcgggatc ttcagcagcg cgtgcggcaa ctgcgacggc catcatttct tgggcagttt
      781 tgaggtccgt cgtcatacgt atgacctacc ggcagatcac agattcgtca aggaaatcgc
      841 cgttctttac agtgattcgg tgtcttgttt gccatgaaag gcatcgatag aatgcggacc
      901 actgatgagt agaactccgc tcgtaatctg tttcggcgat agtctgacgg tagggtttca
      961 gtcgcccgcc aaggacaatc cagtcggaca ggagactccc tatgggcagt tcctgcaatc
     1021 atttttaggc ggcgcggggc aagttcgaat ctctgggatc tgcggtgagt tgacgggcga
     1081 aatggtcaca cgattcaagc gcgatgtgct cgatcatcag ccgggctatg tgccgatcct
     1141 cggaggaacc aacgatctgg ggtggaacgt gccactgtcc gaaatcatag gcaatctagc
     1201 cacgatgtac gagcggacgc ttgcgatggg aagtttgccc attccggtga cggtgccctc
     1261 aatacgagtg gaagacgctt cgggcagccg agaaggactg gagtggatag ccgaccacct
     1321 tgcccgccga agcagattga accaactgat ccacgattat gccgactcca aaggccttgc
     1381 ctatgtcgat cttttcgcag ccaccgtgga ttcgaagagc gggcaattgg aagaactgta
     1441 ctcgaacgac gggatccacc tgaccactgc tggatatcgg ctatttgccg agcatgtcgc
     1501 acgcatcttg aagccactgt tggggcaaga cctgcaaccg tgagctccat gttccaagca
     1561 gttactgatc gagaatttga tgcggtggtc gaggcaagtg ccgtccctgt gttagtggaa
     1621 ttttggaaac caggctgcgg ccattgccga gccttgatgg cggagctcaa acgtttgcaa
     1681 gccgatatgg gatcgaatct tctcattctt acgatgaac
//
LOCUS       DASTWE010000550         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_855097, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000550 DASTWE010000000
VERSION     DASTWE010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855097"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="leuC"
                     /locus_tag="VFT30_05685"
     CDS             complement(<1..1055)
                     /gene="leuC"
                     /locus_tag="VFT30_05685"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU5092155.1"
                     /translation="MAGKTLFDKIWDSHVVRSEPDGTTLLYIDRQLVHEVTSPQAFEG
                     LKLAGRRPRRPAATLAVPDHNVPTTDRRLGIADQVSALQIQTLEDNCKDFGITLFNMS
                     DIRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEIRVDGTLSERCSAKDIILAIIGKIGTAGGTGYVIEYTGSAITALSMEGRM
                     TLCNMSIEGGARAGMVDPEEKTIAYIKGRPLAPTGEFFEQAVRAWRELKTDTDAKCDR
                     TVVLEAKQIAPQVSWGTSPGMVLGVDQNIPDPRTMPDEKTKNATERALAYMGLSPNMP
                     ITGIKIDKVFIGSCTNSR"
     gene            1131..1637
                     /locus_tag="VFT30_05690"
     CDS             1131..1637
                     /locus_tag="VFT30_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HEU5092156.1"
                     /translation="MPCLARRVTTTLVKTAPWTEPRVVEWSQLLLDSYRHWIGKDLIE
                     RTPNVKQQARLLFEASFVVVSHGAEPDPILNYGNQTALDLWELSWDQFIKTPSRLTAE
                     PDDRAERGSMLERAKLNGYFDGYRGVRISSTGRRFLVEQALIWNVIDPAGSPIGQAAT
                     FSQWSNVS"
BASE COUNT          461 a          550 c          543 g          478 t
ORIGIN      
        1 cgcgagttcg tgcaggatcc gatgaacacc ttgtcgattt tgataccggt aatcggcata
       61 ttgggagaga gccccatata agccaatgcc cgttcggtgg cattcttcgt tttctcatcc
      121 ggcatcgttc ggggatccgg aatattctgg tccacaccaa gtaccatacc gggactggtg
      181 ccccagctga cttgcggggc gatctgtttc gcttccaata cgacagtcct atcacatttg
      241 gcatccgtat ccgtcttgag ctctcgccat gcccgaacag cctgttcgaa aaactcacct
      301 gttggagcga gtggccgtcc cttgatatag gcaatcgttt tctcctctgg atcgaccatg
      361 cccgcacgag ccccaccttc aatcgacatg ttacagagag tcatacgacc ctccatgctt
      421 aatgcggtga ttgcagaacc ggtatattca atgacatagc cggtcccccc ggctgtcccg
      481 atcttcccaa tgatcgcaag aataatgtcc ttggccgagc aacgctcgga gagagttcca
      541 tcgacgcgta tttccatggt tttggggcgc ttctgcacca aacactgagt ggccagcacg
      601 tgttccactt cacttgtccc gatgccgaac gccaaggcac caaacgctcc atgggtggac
      661 gtgtgggagt cgccacagac gatcgttgta ccagggaggg tgaagccctg ttccgggccg
      721 atgacatgga cgataccctg gcggatgtcg ctcatgttga aaagggtgat accgaaatcc
      781 ttgcagttgt cttcgagtgt ttgaatctga agagcgctca cttgatcggc gatcccgagc
      841 cgccgatctg tggtggggac attatggtcc ggcacagcga gtgttgcggc aggtcgtcgt
      901 gggcggcgtc ccgcaagttt taacccttcg aaggcctgag gggaggtcac ttcatgaacc
      961 agttgtcgat ctatatatag cagtgtcgtt ccgtccggct ctgatcggac gacatgcgaa
     1021 tcccaaattt tgtcgaacaa agtcttgccc gccatctgta ctcctgtttc gttaaacgag
     1081 agcccattat acagaggaat cccatggtct tgacaaggtg tctgtgaagc ttgccctgcc
     1141 tcgcaagacg tgttaccacc acgctcgtga aaactgcgcc gtggactgaa ccgcgtgttg
     1201 tggagtggag tcagctgttg ctggacagct atcgtcattg gataggaaaa gatctgatcg
     1261 agcggacacc caacgtgaaa cagcaggcac gtctgttgtt cgaagcatcc tttgtcgtcg
     1321 tgtcgcacgg tgcggagccg gacccgatct tgaactatgg caatcaaacc gccttggacc
     1381 tgtgggaact gtcctgggat caattcatca agacgccgtc gcggctcacg gccgagcccg
     1441 atgaccgtgc tgagcgagga agcatgttgg aacgagcaaa actcaacggc tacttcgatg
     1501 ggtatcgggg agtgcgaatc tcttcaacgg ggaggcggtt tcttgtcgaa caagccttga
     1561 tctggaacgt gatcgatcct gccggaagcc ctatcggtca agccgcaaca ttctcacaat
     1621 ggtcgaatgt gtcttgatcc ggaagacctc actttccgga ttggtcacgg cgactgccat
     1681 tgaatcgagt cgagcaatga acggtacggg aaaaatggtt acacaagcgt ctctctttga
     1741 gaacaacagc cctccctttt aatagaaccc gaataatgag cgcagcgcag ctcccagttt
     1801 caggtagccc tcgcaaatcc cactgagtag tccgctcact tgcttgggcg gcgcacacct
     1861 tgcatgaaaa tgaataagaa gatggcgatg ccttccggct tgatctacag gctacaaacg
     1921 gagaccccat gatcttctat gaattctcgc ttaactgaaa atcatggcgg gaggcgaggt
     1981 ttaacactcc gcaacacagg cgttcgcacg gattttaact gattaattag gc
//
LOCUS       DASTWE010000551         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1501555, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000551 DASTWE010000000
VERSION     DASTWE010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501555"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFT30_05695"
     CDS             complement(<1..973)
                     /locus_tag="VFT30_05695"
                     /inference="COORDINATES: protein motif:HMM:NF016452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092157.1"
                     /translation="MIKERMSIRDQEQLSPVTLVNIRPVVAALREFFGSSQLSQFMDQ
                     TNPLAELRHKRTLSALGPGGLRRERAGFDVRDVHHSHYGRICPIETPEGPNIGLIGRL
                     ASFSRVNEYGFIETPYRKVFRTMPADDDRLEGRTLRENVTDPKSGEVLFKSGETIDTK
                     MMKRIARTGAPVGIVPFVSDQFDYLSADAEDKYVIAQANAPLTETKEFVRDRVSCRYH
                     SGFIFSTPDSVDYMDVAPHQVVGISAALIPFLEHDDANRALMGSNMQAQAVPLVRPEI
                     PLVSTGMEYHAALDSGQVVVAEADGEVTSVTGNTITVKERGGVHTYQL"
BASE COUNT          224 a          289 c          295 g          214 t
ORIGIN      
        1 gcagctgata tgtgtggacg ccgcctcttt ccttgacggt aattgtattt ccggtcacgg
       61 acgtgacttc gccgtccgct tccgccacca ccacctgacc ggagtcaagc gcagcgtggt
      121 attccatacc cgtcgagaca aggggaattt ccgggcggac aagcgggaca gcctgcgcct
      181 gcatgtttga acccatcaag gcgcggttcg catcatcatg ctcaaggaat gggatgaggg
      241 cggcactgat acccacaacc tgatgtggcg cgacgtccat atagtccact gaatccggag
      301 tcgagaagat aaagccggag tggtagcggc aggatacccg gtcgcgcacg aattctttcg
      361 tctcggtcag cggggcattt gcctgcgcga tgacatactt atcttccgca tcggcagaca
      421 gataatcgaa ctggtcggag acaaatggaa cgatgccgac aggagcaccg gtcctggcaa
      481 tcctcttcat catcttcgta tcgatcgtct caccgctctt gaacaggact tctccgctct
      541 tcggatctgt gacattctcg cgcaacgtgc gcccttccag ccggtcatcg tcggctggca
      601 tggtgcggaa caccttgcga tagggagttt cgataaagcc atattcgttc acacgtgaga
      661 acgaagcaag gcggccgatc aaaccgatgt tcggaccttc aggggtttcg atcgggcaaa
      721 tacgaccata atgcgagtgg tgtacgtcgc gcacatcgaa gccggcacgc tcgcggcgca
      781 gaccaccggg acccagcgca gatagtgtgc gtttgtgacg cagctcagcc agcgggttgg
      841 tctggtccat gaactgcgaa agttgggagg aaccaaagaa ttcgcgcaac gcggcgacca
      901 cagggcggat attcaccagc gtcaccggtg acaactgttc ctggtcgcgg atagacatac
      961 gttctttgat cacgcgttcc atacggcgca agccgatgcg gagtttgttc tggatcagct
     1021 ca
//
LOCUS       DASTWE010000552         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_532064, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000552 DASTWE010000000
VERSION     DASTWE010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532064"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFT30_05700"
     CDS             complement(<1..174)
                     /locus_tag="VFT30_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5092158.1"
                     /translation="MFDPREPIIRFDHASFGFPGLIALKDISLDIYEGEFVGVIGPNG
                     SGKTTLCRAVLGLL"
     gene            complement(214..1164)
                     /locus_tag="VFT30_05705"
     CDS             complement(214..1164)
                     /locus_tag="VFT30_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEU5092159.1"
                     /translation="MVPSPPMFPVNHVRKLPVLSLLTVLVGFLPLFGTITEARDPIPV
                     VVTIPVLKDLAEQVGGPHVRVTSLLSGYENEHTYSPKPTDLVAVRKARLLLEIGMGLE
                     VWVSSLVKNAGGQSLRVITTSQGVELIRDDVDAHEGMHHGGEAGNPHIWLDPENVAIM
                     LRHITEALIEVDPSHTAEYQTNQAGYLQRLGQLQKELTARTQRLSDRRFIAHHPAWPY
                     LAKRFSLDIVGTIHKQSGAEPSAFHLQFLIEKMRKENIRVVVSEIQLSQRLPELLARE
                     TKAHVVVLTTMPGGLPGTETYLDMLRHNVLQLASALERTS"
     gene            1304..>1580
                     /locus_tag="VFT30_05710"
     CDS             1304..>1580
                     /locus_tag="VFT30_05710"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU5092160.1"
                     /translation="MNVVGLMSGTSADGIDAALVTIDRQKVGLHVEMVAFHSLSYPRS
                     LQQRILSASVSGTVADICHLNALLGEWFADAALGVIRSAQWPPEDVDL"
BASE COUNT          364 a          423 c          448 g          345 t
ORIGIN      
        1 gagaagtccc aggacagcac ggcaaagcgt cgtcttgcct gatccatttg ggccgatgac
       61 tcccacgaac tcaccctcgt agatatccaa cgaaatatct ttgagcgcga tgagcccggg
      121 aaacccgaat gaggcatggt cgaaacggat gatcggctcg cgggggtcaa acacggaaat
      181 ccgaagctcc ttccggcagt aagataagaa aggttacgag gtcctttcca acgcactggc
      241 caattggagc acattatggc gaagcatgtc gaggtaggtc tcggttcccg gcaaaccgcc
      301 tggcatcgtc gtcaagacga cgacgtgggc cttggtttcc ctcgccagta gctccggaag
      361 tcgctggctg agctgaatct cggatacgac aaccctgata ttctcctttc gcatcttctc
      421 gatgagaaat tgtagatgaa aggcggatgg ttcagcaccg gactgcttat ggatcgtgcc
      481 gacaatgtct aggctgaatc gctttgccaa ataaggccag gccggatgat gggcgatgaa
      541 acgccggtcg gatagccgct gagtacgcgc ggtcagttct ttttgcaact gaccaagccg
      601 ttgaaggtag cccgcttgat tggtctggta ttcagccgta tgacttgggt ccacttcgat
      661 gagggcttcg gtgatatggc gcaacataat ggcgacattc tcagggtcca gccaaatatg
      721 cggattccct gcttccccac cgtgatgcat cccttcatga gcgtcgacat cgtctcgaat
      781 caactctacc ccttgggatg tcgtgatcac acgtaacgat tgacctcccg cgtttttcac
      841 cagagatgaa acccagacct ccagtcccat gccgatctcc aacaatagtc tggccttccg
      901 gactgcgact aaatcggtgg gtttgggtga ataggtatgc tcgttttcgt agccgctcaa
      961 caaagacgtg actcgtacat gaggaccacc gacctgctct gccaaatcct tcaggacggg
     1021 aatcgtgacc acaacgggaa tggggtcgcg agcttcggta atggtgccaa agaggggaag
     1081 gaagccgact agcactgtga ggagagagag gacgggcaac ttccgcacgt ggttgaccgg
     1141 aaacatcggc ggggacggta ccatccgcgc attacggtgt caacgacacc atgagggcga
     1201 cacgacgcaa taggccctgt ccattcgact ccttgaccat acaccaaagt tcgattagcg
     1261 tagcaccccg tgagttcgtg cgggtggcga aaggatacca gggatgaacg ttgtcggatt
     1321 gatgtcagga acttcagcag acgggataga tgcggcgctg gtcaccatag accgtcagaa
     1381 agtcggcctc cacgtcgaga tggtggcatt tcattcgctt tcctatcctc gttcgttgca
     1441 gcaacgaatt ctctcggcat cggtttccgg gacggtggcg gacatttgtc acctgaatgc
     1501 actcttaggc gaatggtttg ccgacgccgc cttgggagtc attcgatcgg ctcaatggcc
     1561 cccggaggac gtagatctga
//
LOCUS       DASTWE010000553         1397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_144270, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000553 DASTWE010000000
VERSION     DASTWE010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144270"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFT30_05715"
     CDS             <1..675
                     /locus_tag="VFT30_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092161.1"
                     /translation="GKPSFHVPGSDDLSEGTMTFMSHVETDQFAHIRRMRDSRFPHEI
                     ASILPGEFFVSATPMVIYTVLGSCISACIRDPIVRVGGMNHFMLPEPKEAEHDSWGES
                     TRYGSFAMESLINAILKRGGIKNRLEIKLFGAGKIYDSQIDVGDENAKWVIKYLKSEG
                     LSVSNADLGNVFPRKVYYFTESGRVLLKKIERVKNRTIFEREHAYAARIQQREQQQPG
                     DDVTLF"
     gene            751..>1397
                     /locus_tag="VFT30_05720"
     CDS             751..>1397
                     /locus_tag="VFT30_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5092162.1"
                     /translation="MKKIRVLTVDDSALMRQVLAMLLAKDPQIEVVGSAPDPYIAREK
                     IKALNPDVLTLDVEMPKMDGLTFLEKLMRGHPMPVVMVSSLTEAGCQTTFRALELGAV
                     DFITKPKIDLREGMEDIAQDLIAKIKAASVARIRGQNGGGRVDQKVSPSPLSRTSSTA
                     MIKTTDTIIAIGSSTGGTEAVKEVLERLPPHTPPILITQHMPERFTKTWADRLNTL"
BASE COUNT          344 a          342 c          404 g          307 t
ORIGIN      
        1 ggtaagccat cctttcatgt ccctggctcc gacgatctat cagaaggaac gatgacattc
       61 atgtcacacg ttgagaccga tcagttcgcc catatccggc ggatgcgcga cagtcgattt
      121 cctcacgaaa tcgcttcgat cttaccgggc gagttcttcg tcagcgcgac gccaatggtc
      181 atttacacgg tgctcggttc gtgcatctcg gcctgcatcc gagatccgat tgtccgagtg
      241 ggaggtatga accacttcat gctgccggaa ccgaaagaag cggaacatga ttcgtggggg
      301 gagtcgacgc ggtatggctc ctttgcgatg gagtccctca tcaacgcaat cctaaagcgc
      361 ggcggaatca aaaaccgttt agagatcaaa ctgtttggag cgggcaagat ctatgacagc
      421 cagatcgacg tgggcgatga gaatgcgaaa tgggtgatta aatatctgaa gagcgaaggc
      481 ttgtccgtat cgaacgcaga tctgggaaat gtgtttccaa gaaaggtgta ctacttcacg
      541 gagtctggtc gtgtgctctt gaagaaaatt gagcgggtca agaatcgcac tatctttgaa
      601 cgtgaacatg cctatgcagc gcgtatccag caacgtgagc aacaacaacc tggagatgat
      661 gtgacactgt tctgaattgg gaacagcgcc gctcacatct cattctggac ggatttaaag
      721 gagatccatt ttgcgggatg tgaggaaccg atgaaaaaga ttcgagtctt gacggtcgat
      781 gactcggcgc tgatgcgaca agtgctagcc atgttgcttg cgaaagatcc acaaattgag
      841 gtcgtcggat cggctccgga tccgtatatc gcccgcgaaa aaatcaaggc gttgaatccc
      901 gacgtgctca cccttgacgt cgagatgccc aagatggacg gtctcacatt cttggaaaag
      961 ctgatgagag ggcatccgat gccggtggtg atggtgagtt cgttgaccga ggccggctgt
     1021 cagaccacgt tccgcgcttt ggagcttggt gccgtggatt ttatcaccaa acccaagatc
     1081 gatctccggg aaggcatgga ggatattgcg caggatctga tcgcgaagat caaagccgct
     1141 tcagtggcgc ggatcagagg gcagaacgga ggaggtcggg ttgatcagaa agtaagtccg
     1201 tcgccgttgt cacgcacgtc ctccaccgcg atgatcaaaa cgaccgatac catcatcgcg
     1261 attggttcat ccaccggtgg gacggaggct gtgaaggagg ttttggaaag gcttccgccg
     1321 catacaccgc ccatcctgat cacgcaacat atgccggagc ggtttacgaa gacttgggcg
     1381 gatcggctga acaccct
//
LOCUS       DASTWE010000554         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1566121, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000554 DASTWE010000000
VERSION     DASTWE010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566121"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..942
                     /locus_tag="VFT30_05725"
     CDS             34..942
                     /locus_tag="VFT30_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5092163.1"
                     /translation="MTLTELQYIVAVARERHFGRAAERVFVTQPALSLAIKKLEEELG
                     TTIFERRQARIELTALGEQIVYQAQRVLEEAEQIKILAAQGKDQLNGLLRLGVIATVG
                     PYILPDLVPLLSAHAPKMPLELEENLTQNLAGMLKNGRLDVIMIAFPFEEPGVLTSAL
                     YDEPFQAVVPAGHPWRKKKFLDSRQLVAEKVLLPHAGHCFRQQVLDVCPELSRSDAEG
                     WQGNSLETIRQMVVSGLGITVLPCSALTPKYQNKRLIAIKLADPAPGRRIGLAWRRGF
                     TRPQVIDVIREAVYALKVPGLKMVTE"
     gene            1027..1800
                     /gene="xth"
                     /locus_tag="VFT30_05730"
     CDS             1027..1800
                     /gene="xth"
                     /locus_tag="VFT30_05730"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5092164.1"
                     /translation="MKIATFNVNSLRKRLPIVLEWLDRHQPDVLCLQETKVQDSEFPL
                     LALAPSGYETTFRGMKSYNGVAILSRKKPEAVFYGFDDGKDPEDSRLLRVLIDGIPII
                     NTYVPQGFEIDSPKYTYKLAWYERLRDYFNTHLSPQAPAIWCGDMNVAPRPMDVHSPE
                     KHLNHVCYHEAARHAYEKTIAWGFQDVFVKLYPTRQQYTFWDYRAPSSLEANKGWRID
                     HMMATAPLAEKCVKADVDVEPRRAKEPSDHTFLWAEFSI"
     gene            complement(1879..2034)
                     /locus_tag="VFT30_05735"
     CDS             complement(1879..2034)
                     /locus_tag="VFT30_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092165.1"
                     /translation="MTCVKCKGLMVHERQPAVSSSTIILRCLNCGLIIDPLIEQNRNN
                     HMRAKAA"
     gene            complement(2134..>2525)
                     /locus_tag="VFT30_05740"
     CDS             complement(2134..>2525)
                     /locus_tag="VFT30_05740"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU5092166.1"
                     /translation="IGLRSSTIRDAKGTETLIPNSSFLERALTNWTYSSRVGRFSLRV
                     GAPYDRAAEQIMNLLSALALQHPKVMKQPAPQVLLEEFGAQARIFTVNYWHEISPDID
                     PSGIASELRFAIEQKFAEAGLKVLPAT"
BASE COUNT          604 a          675 c          684 g          562 t
ORIGIN      
        1 gaacaataag aataacttat taccgaacga tcaatgacgc tgacggaact gcaatatatc
       61 gtcgccgtgg ctcgagaacg ccatttcggc cgtgccgctg agcgtgtatt cgtgacgcaa
      121 ccggctttaa gcctcgcgat caaaaaactg gaagaagaat taggcacgac tatttttgag
      181 cggcgccaag cccggattga actgacagcg ctcggggagc aaatcgtcta ccaagcacaa
      241 cgcgtattgg aagaggcgga gcaaatcaag attctggcgg cgcaaggcaa agatcaattg
      301 aatggactgt tgcggctcgg cgttatcgcg accgtggggc cctatatcct tccggatttg
      361 gtcccactct tgagtgcgca tgcaccaaag atgcccctgg aattggagga aaatctcact
      421 cagaacttgg ctggaatgtt gaagaatggg agactcgatg tcatcatgat cgcctttccc
      481 ttcgaggaac caggagtact cacaagcgcc ttgtatgatg aaccatttca ggccgtagtc
      541 cccgctggac acccctggcg gaaaaagaaa ttcctcgatt cccgtcaact agttgccgag
      601 aaggtcctcc tgcctcatgc gggacattgt ttccgccagc aagttctcga tgtctgtccc
      661 gaactcagtc gctcggatgc ggaaggctgg cagggaaact cgttggaaac catccggcag
      721 atggtcgtct ctgggttggg catcaccgtg ctgccctgca gcgccctcac accgaaatat
      781 cagaacaagc gtctgatcgc gatcaagctg gcagaccccg cgcctggtcg tcgaatcggt
      841 ctcgcgtggc ggcgtggatt cacacggcct caagtcatcg acgtgatccg agaggctgtc
      901 tatgcgttga aggtaccggg tctcaagatg gtgacggagt gacggacatg actcaacgct
      961 tcccaaccgc ttgttgccct ggacggcttt tgttaggatg cgccgactcg ttggagcgca
     1021 cgtgggatga aaatcgcgac gttcaacgtc aattcattgc gcaaacgttt gccgatcgtg
     1081 ctcgagtggc ttgatcgaca tcagccggat gtcctgtgcc tccaagaaac caaggtccag
     1141 gatagcgaat ttccattgct cgcgctggct cccagcgggt atgagacgac ctttcgagga
     1201 atgaaatctt ataacggggt ggcgatcctc agccgaaaga agccggaagc agttttctat
     1261 ggattcgacg atggaaaaga ccccgaggac tctcgcttgc tgagagtcct catcgatgga
     1321 attcccatca tcaatactta tgtgccgcag gggttcgaaa ttgattcgcc gaagtatacc
     1381 tataagctcg cctggtacga acgactacgg gactatttca acacacatct ttcaccgcaa
     1441 gccccggcta tctggtgcgg agatatgaac gtggctccac gccccatgga cgtccacagt
     1501 cccgagaaac atctgaacca tgtctgctac cacgaggccg cgcgccacgc atatgaaaag
     1561 acaatcgcgt ggggatttca ggatgtcttt gtgaagcttt acccgacacg gcaacaatac
     1621 acgttctggg actaccgtgc gccgagctca ttggaggcca acaaaggatg gcgcatcgat
     1681 catatgatgg ccacggcacc gctggcggaa aagtgcgtga aagcggatgt ggacgtcgag
     1741 ccgcggcgag cgaaagagcc ttcagaccat acttttttgt gggctgagtt ttcgatctga
     1801 ctgcgcacgc gacgcccaat ccgaccgcgc atataaattc ccccagcctc cggccccggc
     1861 ccaaagaaaa gctgtctcct aggccgcctt ggctcgcatg tggttgttgc gattctgctc
     1921 aatcaaggga tcaatgatca ggccgcaatt gagacagcga agaatgatcg tactggacga
     1981 cactgccggt tggcgctcgt gaaccatcaa gcctttgcat tttacacagg tcattggcat
     2041 ccctccttgc cgatattgac tgcgcacgat atgtaacatt ccaagattag tggcatgtta
     2101 acagccagtt aaaaatctct aatgccgcga ttatcatgta gccggcaata ctttcagacc
     2161 ggcttccgca aacttttgtt cgatcgcgaa acgcaattcg cttgcgattc cgctcggatc
     2221 gatgtcaggg gagatttcat gccaatagtt caccgtgaag atacgagctt gcgctccgaa
     2281 ttcctcgaga agcacttgcg gtgcaggttg tttcataact ttcggatgtt gcagggcaag
     2341 agctgaaagc agattcatga tctgttcggc agccctatca tatggagcgc cgactcgcag
     2401 agaaaaccgc ccgacacggc ttgagtaagt ccaattggtg agggcccgtt cgaggaaact
     2461 gctgttcggt atcagcgtct ccgttccttt ggcgtctcgg atggtggatg aacgaagacc
     2521 gatcg
//
LOCUS       DASTWE010000555         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1598735, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000555 DASTWE010000000
VERSION     DASTWE010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598735"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1061
                     /locus_tag="VFT30_05745"
     CDS             <1..>1061
                     /locus_tag="VFT30_05745"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU5092167.1"
                     /translation="SDPAVNNPAGTGTPASTSPSAGPAMSYADVVSKAAPAVVTIHSQ
                     MRVRQPQQYPFGNDPFFREFFGDRVPQQAPERRREGLGSGVIVAQDGYVLTNHHVIDG
                     ADRIKVDLNDNRTLEAKVVGSDPPSDLAVLKIEASGLPVLALGDSDRTRVGDVVLAIG
                     NPLGVGQTVTMGIISAKGRQTGLSDGSFEDFLQTDAPINQGNSGGALVSTNNELVGIN
                     SQILSPSGGSIGLGFAIPSNMARSVMDQLINTGKVRRGQLGITVLKIPSEEASKLGVD
                     QGPGVVVYQVQPGSAADRAGLRKGDVITALNGTAVNDQNSFRNVIAGIAPGSEVTLTV
                     KRDGREQQVRATLDEFTPQ"
BASE COUNT          240 a          277 c          325 g          219 t
ORIGIN      
        1 cttcggaccc ggcagtcaac aacccggccg gcacggggac accggcttca accagccctt
       61 ctgccgggcc agccatgtcc tacgccgacg tggtgagcaa agcggcgccg gcggtggtaa
      121 cgattcattc ccaaatgcga gttcggcagc cgcagcaata tccatttggg aacgatccgt
      181 tctttcggga gttcttcggc gatcgtgttc cacagcaagc gcctgaacgg cggcgagaag
      241 gattaggttc gggcgtgatc gttgctcagg acggttatgt tttgacaaac catcatgtca
      301 ttgatggggc cgatcgaatc aaggtggatt tgaatgacaa tcgcacactc gaggctaaag
      361 ttgtcggctc cgatccccca agcgatctgg cagtactgaa aatcgaggcc agtggcctcc
      421 cagtgttagc gctgggcgat tcagatcgca ctcgagtcgg cgatgtagtg cttgccatag
      481 gaaatcctct gggtgtaggt cagacagtta ccatggggat catcagcgcg aagggccggc
      541 agacggggct gagcgatggc agctttgaag attttctgca gaccgacgca ccgatcaatc
      601 aaggtaattc aggcggggcc ttagtcagta ccaacaacga gctggtcgga atcaactcgc
      661 aaatcctttc accgtcggga ggcagcatcg ggttgggatt tgcgatcccc tcaaacatgg
      721 cccgcagcgt gatggatcaa ttgatcaata caggcaaagt aaggcgtggg caacttggga
      781 tcaccgtgct caagatccct tcggaagaag cgtcgaagtt gggtgttgat cagggccctg
      841 gagtggtggt ttatcaggtt caacctggaa gtgctgctga tcgggctggg cttcgcaagg
      901 gggacgttat cactgcgctc aatggaacgg ctgttaacga tcaaaactct tttcgtaacg
      961 tgatcgccgg catcgctcca gggtcggagg taacgctcac cgtgaagcgc gacggaagag
     1021 agcaacaggt gcgtgcgacc ctcgacgaat tcaccccgca g
//
LOCUS       DASTWE010000556         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1533979, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000556 DASTWE010000000
VERSION     DASTWE010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533979"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFT30_05750"
     CDS             <1..537
                     /locus_tag="VFT30_05750"
                     /inference="COORDINATES: protein motif:HMM:NF044298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092168.1"
                     /translation="GVPVQLKLESELPWNGKVLIHVRPEATSQFTIHLRKPSSEGLSP
                     SGEQTASGYDPRSADYETITRIWSAEGETLEFKFDTSIRLCRAHPKVKGHAGKVAITR
                     GPLVYCLESVDQPHLDIFSVQLDPSSLREEYMTDLLGGCVVIEGKTTDGRPLKFIPYF
                     MWGNRGESQMTVWIHGTL"
     gene            534..>1107
                     /locus_tag="VFT30_05755"
     CDS             534..>1107
                     /locus_tag="VFT30_05755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5092169.1"
                     /translation="MRGNAAEFDTQRDDVFGRIASRYDLLCDLFSLGIHRLWKRQVAQ
                     MIAKEPWSQLLDCATGTGDIVLCVLAHREIGQGQRIIASDISPQMLAIAQKRLAAFNS
                     SVELGVLDAHSMPEVQAESIDLYSISLGLKICDRKKVLREALRLLRPGGRLVVLEASN
                     ISWRWLHRAYLFYMKLCMPVIGWIATRGDSS"
BASE COUNT          250 a          276 c          307 g          274 t
ORIGIN      
        1 ggcgtacctg tgcagctcaa actcgaatcg gaactgccgt ggaatggaaa agtattgatc
       61 catgtcaggc ccgaagcaac aagccaattc acaattcacc tgcgcaaacc ttcatcagaa
      121 gggctttctc cgtccggaga acaaaccgct tctggctacg acccacgcag cgcagattac
      181 gaaacgatca cgcgcatctg gtccgcggag ggcgagacgt tagagttcaa gttcgacaca
      241 tccatcaggt tgtgccgtgc ccaccccaaa gtaaaggggc atgccggcaa agtcgccatc
      301 acgcgcggtc cactggtcta ttgcctggaa agcgtcgatc agccccatct tgatattttt
      361 tccgtgcagc tcgacccttc ctctctccgc gaagaatata tgaccgatct tttaggtggg
      421 tgtgttgtta ttgaaggcaa gacaacagac ggcaggccgc tgaagttcat tccgtatttc
      481 atgtggggga atcggggtga gtcacaaatg accgtgtgga ttcacggaac gttatgagag
      541 gcaatgcggc ggagttcgac acgcagcggg atgatgtctt tggacgaata gcgagtcgtt
      601 atgacctgct ctgtgatttg tttagtcttg ggattcatcg cctttggaaa cggcaggtgg
      661 ctcaaatgat cgcgaaagaa ccctggtcac agttgctcga ctgtgccacg gggaccgggg
      721 atattgtcct gtgtgtgctt gctcataggg aaattggtca aggacagcgg atcattgcgt
      781 cggatatcag cccccaaatg cttgctattg cacaaaagag attggctgct ttcaattctt
      841 ccgtggaact cggtgtgctc gatgcgcatt ccatgccgga ggttcaggct gaaagcattg
      901 acctttactc catttcgctt ggactcaaga tctgtgaccg caaaaaagtc ctgcgggaag
      961 cgttaaggct cttgcgtccg ggcggcagac tggtagtgct ggaagcctcc aatatttctt
     1021 ggcgctggct tcaccgtgcg tacctgtttt atatgaagct ttgtatgcct gttatcggct
     1081 ggatcgcgac acgcggagat tcttctg
//
LOCUS       DASTWE010000557         1427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_241462, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000557 DASTWE010000000
VERSION     DASTWE010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241462"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VFT30_05760"
     CDS             <1..1211
                     /locus_tag="VFT30_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5092170.1"
                     /translation="DMRTPGVHIRQHCQFEGCRGIENAHMTFTDVHIPTENVIGEVGR
                     GLKYALTILNVGRAVSIPAICLGMAKQAWQPTLDRANQRLTFQKPLGTRQTQRMRLGR
                     MASNLFAMEALALTAWRMADQHTYDVRIEAALAKLFCSEKTIQFLKDAQIIFGGMGYE
                     TAHSKWIRGEPAFGIEQLVRDAEMYRIGEGATDILRPFVAREGLNMHLERVGRLFDER
                     TTGVHRLIELQRLLRFYAPWYVSQWKSGRLPPRAEFHHPMVRSKLIFVERASRRLART
                     IFYAMLLHRQALRDDQGRQNRIEMIGEDLLVIATTALYAEAQVQTAAHPEVWELVEEC
                     FREAKQRVEQDIKGLIRNQDTAVTAVGEKALSGRYPSLISGIIQRNLQNYSRHESILK
                     ADDANMHTVV"
BASE COUNT          339 a          404 c          397 g          287 t
ORIGIN      
        1 ttgatatgcg gacacctggt gtccatattc gacagcactg tcaatttgaa ggctgccggg
       61 gcatcgagaa cgcccacatg acttttacgg atgtgcacat cccgacggag aatgtcatcg
      121 gtgaagtggg ccgtggactc aagtacgcct tgaccattct caacgtaggt cgagcggtca
      181 gcatcccggc gatttgcctg ggcatggcca aacaagcatg gcaacccacg ctcgatcggg
      241 ccaatcagcg actcaccttc cagaagcctc tcggtaccag gcagacacag cgcatgagac
      301 tgggccgcat ggcgtcgaac ctcttcgcta tggaggccct cgcgctcact gcatggcgca
      361 tggcagatca gcatacctat gatgtccgga tcgaggcagc cctggccaag ctcttttgct
      421 ccgagaagac cattcagttc ttaaaagacg ctcaaatcat cttcggaggg atgggatacg
      481 aaaccgccca ctctaagtgg attcgtggag aacctgcgtt cggcattgaa cagctcgtcc
      541 gcgacgcgga gatgtatcga atcggagaag gtgccacgga tatcctacgc ccgttcgtcg
      601 cgcgtgaagg cctcaatatg catcttgagc gggtcggacg cctcttcgac gaacgaacga
      661 cgggcgttca tcgacttatt gagttgcagc gactcctgag attctatgct ccttggtacg
      721 tgagccaatg gaaaagcggc cgattgccgc ctcgcgcaga gttccatcat ccgatggtcc
      781 gctccaaatt gatcttcgtg gaacgcgcga gtcgtcgtct cgcccggacg atcttctatg
      841 ccatgctgct ccaccgccaa gcgcttcgag acgatcaagg ccggcaaaac cggatcgaga
      901 tgatcggaga agacctgttg gtcatcgcaa cgaccgcact gtatgccgaa gcacaggtac
      961 agacggcagc ccatccggaa gtttgggagc tcgtagaaga atgctttcga gaagcgaagc
     1021 aacgtgtgga acaagacatc aagggactga tccgcaatca ggataccgcc gtgacagccg
     1081 ttggggaaaa ggccctcagc ggtcgatatc cttcactcat cagcggaatc atccagcgca
     1141 atcttcaaaa ctactcgcgc catgagagta ttctcaaggc agacgatgcg aacatgcaca
     1201 cggtcgtctg attcaatggg acgtctctgg aacatcgtca cggtcaccca tcataataaa
     1261 gctgatcaaa acaaaggcaa cctatgcgcg cctggttgat ggactcctat gccggagttg
     1321 aacagttgcg gcttgacgag gtgccggatc ctcagccagg cccgtcagag gtgcggctca
     1381 aggtgaggta tgccgcgctc aatcccgcgg atgcatttct ggcgcaa
//
LOCUS       DASTWE010000558         1189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_596535, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000558 DASTWE010000000
VERSION     DASTWE010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596535"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..>1189
                     /locus_tag="VFT30_05765"
     CDS             68..>1189
                     /locus_tag="VFT30_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092171.1"
                     /translation="MSLLIASYLLEYHNIRLLAPLSTMLKGNPGKQRLSILRIQPSLT
                     ITIEEITLLVNALAEVLEIIARNNEYCLIAHLAGTHVPESIRRSPKHMEVGWPIVTEH
                     RHIDARAGFIIHPTTVDNLIEYYFPSFNCYPSIKDGLVTWWSRIARFLEPIHVRSTLV
                     SSNGFVVENNLVFVPYLPDSMVSTTTPYLVQEIRDKIQDAVTLAKELGDDNIPVSMVG
                     LGAYTSIVTRNAELINDYEVPVTTGNTYTTALTICGIIHAAKLRGIDLRHATVAVVGA
                     SGYIGSVVSEILAARVGRLKLVGRPRKDHRLRLKFVRELCWRELLRVLRSNTKELDGP
                     TYTPTGDLLTQVANIIEKYREQFVQHNPSLFRMPDDAPLP"
BASE COUNT          291 a          323 c          290 g          285 t
ORIGIN      
        1 cggtgtggaa ttttccccgc tggacgatca ttccccattt tttcgttcgt gcggaaaaca
       61 gggtgtcttg tcgctcctca ttgcgagcta tttactcgaa tatcacaaca tcagactgct
      121 agccccgttg tcgaccatgc tgaaaggcaa tccgggaaaa caacgtcttt ctattcttcg
      181 cattcaacct tcgctcacga taaccatcga agaaatcacg ctccttgtga atgcattggc
      241 cgaagtgttg gaaatcatcg cgcgaaacaa tgagtattgc ctgattgccc atctggcagg
      301 aactcacgta ccagagtcga ttcgccgttc accgaagcac atggaggtgg gatggcccat
      361 cgtcacggaa catcgccata tcgatgcaag ggccggcttc attattcacc ccaccaccgt
      421 cgacaatttg atcgagtact actttccttc gttcaactgt tacccctcaa tcaaagacgg
      481 cttggtcaca tggtggagtc gcatcgcacg gttcctggaa ccgattcatg tccgtagcac
      541 cttggtttca tcgaatggct ttgtcgtgga aaataacctg gtctttgtcc catatctgcc
      601 cgattccatg gtcagcacaa caacacccta cttggtgcaa gaaatacgcg acaaaatcca
      661 agatgccgtc acgcttgcaa aagaattggg cgatgacaat attcccgttt cgatggtcgg
      721 acttggagcc tatacatcaa tcgtgactcg gaatgccgaa ctcatcaatg attatgaagt
      781 tccggtgacg acaggcaata cgtatacgac agcgctgacg atctgtggca tcatccatgc
      841 cgccaagttg cggggcatcg atttgcggca tgcaacggtt gccgtcgttg gagcgtcggg
      901 gtacattgga agcgtagtgt ctgaaattct ggccgcacgt gtcggacgat taaaactcgt
      961 aggcaggccc cgcaaggacc atcggctgcg gttgaaattc gtgcgagaac tgtgttggcg
     1021 agaactctta cgggtcttga gatcgaatac caaagagctg gacggcccaa cctacacgcc
     1081 cacaggcgat ctcttgaccc aagtggccaa cataatcgag aagtatcgag aacagtttgt
     1141 ccagcacaac ccgtcactct ttcgcatgcc ggatgacgca ccgcttccg
//
LOCUS       DASTWE010000559         1564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_952141, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000559 DASTWE010000000
VERSION     DASTWE010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952141"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="purF"
                     /locus_tag="VFT30_05770"
     CDS             <1..1000
                     /gene="purF"
                     /locus_tag="VFT30_05770"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5092172.1"
                     /translation="VIIHLIAHSRAGSFLARVIDALNQVRGAFSVVLMTDNGLIAARD
                     PYGLRPLCIGRLRSSWIVASETCAFDLLDAEYVREVEPGELIVISDQGLDSHHPFPKK
                     NPAMCVFEYVYFARPDSRIFGANAVYATRKALGRQLAQESWVPADVVIPVPDSGVPAA
                     LGYSEWAGIRFETGLIRNHYVGRTFIEPEQSIRHFGVKVKLNAVPEVLDGKRVVVIDD
                     SLVRGTTSRKIVKMIRQAGAKEVHMRISSPPIISPCFYGIDTPTKKELIASDHSIEET
                     RKYITADSLAYLSLDGMLKSAPRTPDQYCTACFTERYPIPFTRAEEIQLGLFESAR"
     gene            1021..1371
                     /locus_tag="VFT30_05775"
     CDS             1021..1371
                     /locus_tag="VFT30_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5092173.1"
                     /translation="MAPPQKDDKKAQPRGRPRVQVDYPASFAGNEGSGHGTVTNLTIA
                     GCEIESQVQLPIGAGLSLHVQTPEARPPIIIALAIVRWKNGDRFGLEFVRFEGGTKGQ
                     LEDMLNQREGPVED"
     gene            complement(1375..>1564)
                     /locus_tag="VFT30_05780"
     CDS             complement(1375..>1564)
                     /locus_tag="VFT30_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092174.1"
                     /translation="SSPNASASRPISTSLSTNTHDLTSAGMIPVVMALPLSLLKLISY
                     WMLHPTMAIFGAATIGRG"
BASE COUNT          343 a          411 c          455 g          355 t
ORIGIN      
        1 ggtcattatc cacctcatcg cacactcgcg agcagggtcc tttctcgccc gggtcatcga
       61 tgctctaaat caagtacgag gggcgttctc ggttgtgttg atgaccgaca acggtcttat
      121 cgccgcgcgt gatccctacg gcctcaggcc gctgtgtatc ggacgtctgc gtagcagctg
      181 gattgtggcg tccgagacct gtgcatttga cttactcgac gccgagtatg ttcgagaggt
      241 ggaaccgggt gagctgatcg tcatcagcga tcaaggactc gacagccacc atccgtttcc
      301 caagaaaaat ccggcgatgt gtgtgttcga atacgtctat tttgctaggc ctgacagccg
      361 catcttcggt gccaatgccg tctatgcgac gcgtaaggca ttggggcggc agttggctca
      421 ggagtcatgg gtgccggcgg atgtcgttat tcctgttcct gactccggcg tgccggcggc
      481 tcttggctat tccgagtggg cgggaatccg gttcgaaacc ggtttgatcc gcaaccacta
      541 tgtcggacga acatttattg agcccgaaca gtcgattcgt cacttcgggg tcaaggtgaa
      601 gctgaatgcc gtgcccgaag tgttggatgg gaaacgtgtc gtcgtcatcg acgactcgtt
      661 agttcgaggc acgacaagcc gtaagatcgt caagatgatc cgacaagcag gggcgaaaga
      721 ggttcacatg cggatcagct caccgccgat catctcgccc tgtttttacg gaatcgacac
      781 cccgacgaaa aaagagctga ttgcatctga tcactcgatc gaagaaaccc gcaaatacat
      841 cacggctgac agtttggcgt atctcagcct tgacggcatg ttgaaatccg ccccgaggac
      901 gcccgatcag tactgtacgg cctgttttac cgagcgctat cctatcccat ttacccgcgc
      961 agaagagatt cagttgggcc tgttcgaatc cgcgcgctga acttgctctg cacggaaaac
     1021 atggcgcctc cacagaaaga cgacaagaaa gcgcagccac ggggtagacc ccgagtgcag
     1081 gtcgactatc cggcttcatt cgcggggaat gaaggatcag gtcatggaac ggtgaccaat
     1141 ctcacgattg ccggatgtga aatcgagagt caggtccagc tccctattgg agcaggtctg
     1201 agtcttcatg tgcaaactcc agaggctcgg cctccgatta tcatcgccct cgccatcgtc
     1261 cgttggaaga atggtgaccg atttggactc gagttcgttc gatttgaggg tggaaccaag
     1321 ggacagctcg aggatatgct gaaccagcgt gagggccctg tcgaagatta gttttcagcc
     1381 ccgccctatg gttgccgctc caaaaattgc catggtagga tgcagcatcc aatagctgat
     1441 taatttgagc agcgatagcg gtagtgccat gacaactgga atcatgccgg cgcttgtgag
     1501 gtcatgagtg ttcgttgaaa gggaggtcga gatgggacga cttgcactgg cgttcgggct
     1561 gctg
//
LOCUS       DASTWE010000560         1242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_628955, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000560 DASTWE010000000
VERSION     DASTWE010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628955"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>1242
                     /locus_tag="VFT30_05785"
     CDS             17..>1242
                     /locus_tag="VFT30_05785"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5092175.1"
                     /translation="MADVLIDNGKIAVVGPNLSIPADGRTIHANGKLVMPGFVDLHVH
                     FREPGFEYKETIQSGSAAAVAGGFTTVCCMPNTNPVNDNQAVTEFILERARLAGLANV
                     LPIGAITKGSEGKELAEIGDLRRSGCVAISDDGKPVMNSLVMRRAMEYALAFDLAVVD
                     HCEDLHLAEGGCMNEGLVSTELGLPGIPAAAEDVMVARNLSLSELTGARLHLAHISTA
                     GSVRMVREAKARGIQVTAEACPHHFLLTEELVRGYNTHAKMNPPLRTWTDVQMIKEGL
                     QDGTIDVIATDHAPHATQEKQQDFTEAPFGIVGLETALPLTLGLVEEGILTLEQAIQK
                     LTSAPAAAFGLKKGTLAVGADADVVIVDQQEPWEVDPSKFRSKSRNTPFVGWKMKGRV
                     NTTIVGGRVVFETDPV"
BASE COUNT          287 a          315 c          393 g          247 t
ORIGIN      
        1 aggcgggttt gtcggcatcg ccgatgtgct gatcgacaat ggcaagatag ccgtagtggg
       61 tccaaatctt tccatcccgg ccgacggtcg gaccattcac gccaatggga agcttgtgat
      121 gccggggttc gttgatttgc acgtccattt tcgcgaaccg gggttcgagt acaaagaaac
      181 gattcagagc ggcagtgctg cggcagttgc aggaggattc acgacggtct gttgcatgcc
      241 caacacgaac ccggtgaacg acaatcaagc cgtcacagaa tttatattgg aacgagcacg
      301 gttggctggc cttgcaaacg tgctgccgat cggcgccatc acgaaaggat cggaagggaa
      361 ggaactggcg gagatcggtg acctgcggcg gtccggctgt gtggccatct ccgacgatgg
      421 caagcctgtg atgaacagcc tggtgatgcg gcgagcgatg gaatatgcct tggcattcga
      481 tctggcggtc gtcgaccatt gcgaagatct gcacctggcg gaaggcggct gtatgaatga
      541 agggttggtt tcgaccgagc ttggattacc gggtatccca gcggcggctg aagatgtgat
      601 ggtggcacgc aatctctcac tctcggaact gacaggagct cgccttcatc ttgcacacat
      661 cagcacagcc gggtcggttc gaatggtgag ggaggcgaag gcccgaggga ttcaggtgac
      721 ggcggaggca tgtcctcacc actttctgct tacggaggaa ctggtgcgcg gctacaatac
      781 ccacgctaaa atgaatcctc cattgcgcac ctggaccgat gtgcagatga tcaaggaagg
      841 cttgcaagac ggcacaattg atgtgattgc aaccgaccat gctccgcatg cgacacagga
      901 gaaacaacag gatttcaccg aagccccatt cgggatcgtc ggacttgaga ccgcgctccc
      961 gctgaccttg ggattggtcg aagaaggaat cttgacgctg gaacaagcga ttcagaagct
     1021 gacctcggct ccagccgcgg ccttcggact caaaaaaggg actctcgcgg tcggagcaga
     1081 tgccgatgtc gtcatcgtcg atcaacaaga accgtgggaa gtcgatccgt ccaagttccg
     1141 gtccaagagc cgcaatacgc ccttcgtggg gtggaagatg aaagggcggg tgaatacaac
     1201 tattgtaggc ggcagagtgg tgttcgagac tgatccggtg ga
//
LOCUS       DASTWE010000561         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_790814, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000561 DASTWE010000000
VERSION     DASTWE010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790814"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..340
                     /locus_tag="VFT30_05790"
     CDS             143..340
                     /locus_tag="VFT30_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092176.1"
                     /translation="MKDLNDRDGGNEIESVIRRPDASLLVYTETNFKGRGMAFVSNQR
                     VLDLDKFQIANDIESLRGECK"
     gene            456..620
                     /locus_tag="VFT30_05795"
     CDS             456..620
                     /locus_tag="VFT30_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEU5092177.1"
                     /translation="MLSWAVTFLVVAIIAGVLGLSGVAGTATNIAYVLFVIFVILALV
                     GFLTGRKPIA"
     gene            773..924
                     /locus_tag="VFT30_05800"
                     /pseudo
     CDS             773..924
                     /locus_tag="VFT30_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173261.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          263 a          278 c          308 g          281 t
ORIGIN      
        1 aggataccgt cattcgggcg aatcgcttcc acagttcgga gagaacacgg gggatcccgc
       61 gcaggagggt cgggaagggc gacgttgagt tcatcaagat tcactcggaa gagtatgcat
      121 cgatcacctg aatcgccgaa ctttgaagga tctcaacgac cgcgacgggg gcaacgaaat
      181 cgagagcgtg atcaggaggc ccgatgcctc attgcttgtt tatacggaga cgaactttaa
      241 agggcgggga atggcattcg tctcaaacca acgggtcctg gacctggata aattccagat
      301 tgccaatgac atcgagtcgt tgcggggcga atgtaaatag gaaagaaagg cgaagcctgt
      361 tcacgctggc gatcatcggt agttacggct gaaggatctt caccgatggg aatccaggta
      421 gattacgcat cacactttaa ccaggaggaa gcgccatgct gagttgggct gtcacatttc
      481 ttgtcgttgc cattatcgca ggagtattag gactctccgg ggtggccggg actgcgacca
      541 acatcgccta tgtccttttt gtcattttcg tgattcttgc gctggtcggc tttctcacgg
      601 gtcgtaagcc aattgcctag cggcagattg cgaacatgcc gaaaccttgc gtcgtccgtg
      661 cactgaggct gtgatggtgt gtcatacatg cctggcctaa cctgaaggca ttgtcaattt
      721 acctcccatt ttgcagactg ggcagtatga caaccaaaac acccaactac caatgccatc
      781 gctttcctca gaaatcatca gccacgccac ctggctctat catcgatcct gtttgagcgt
      841 tcgtgaagtc gaagaacttt tagttgaacc tggagtcacg gtgcgtatga aaccgttcgc
      901 cagtggtgtc agaagtttgg gccagcctat gtgcataagg gatggtgtca ctgctcgata
      961 catccgttcc attgtgcgtg cagccggtaa gttgacaata ccataatttt ttgagcgatc
     1021 aattatagac agccagcttg aataactcga atccggagtg tgccatgccc cttttttctt
     1081 gtcttgtttc ccatcagagg ctcggagtac ggggcgcgct tctgtgcctg
//
LOCUS       DASTWE010000562         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1534089, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000562 DASTWE010000000
VERSION     DASTWE010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534089"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..868)
                     /gene="rsmA"
                     /locus_tag="VFT30_05805"
     CDS             complement(47..868)
                     /gene="rsmA"
                     /locus_tag="VFT30_05805"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5092178.1"
                     /translation="MDSPLPPAAIKRLGQNFLIDPNIVRKIVAVTELTPTDTVLEIGP
                     GRGILTEALCRAAGHVTAIEVDPRLHAYLTERQARFSNLTLVLDDAMIYPIEHLPIST
                     IVVANLPYYLSTPLLFRLLDQRNRFPRMVLMLQNEVADRLVAKPGSSGYGVLSVMAQY
                     AAEITKAFRVSAQCFRPKPEVDSALMLLKTKHQRALNQEEEPKFSALVKAAFAHRRKT
                     LVNSLKDQGYEQKSVAAALESLNLSPSTRAEILSIEQFIGLTRRIYGMTRSNLHR"
     gene            complement(872..1159)
                     /locus_tag="VFT30_05810"
     CDS             complement(872..1159)
                     /locus_tag="VFT30_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092179.1"
                     /translation="MVRQQIEELDTLARQTLQDLNTVAGTERVAKWKERTVALITTTV
                     GPLEGQAIARIQPGPSFTNDMVEEFTDLVECYRSPLVILAKRLAQAPPTGS"
     gene            complement(1179..>1557)
                     /locus_tag="VFT30_05815"
     CDS             complement(1179..>1557)
                     /locus_tag="VFT30_05815"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU5092180.1"
                     /translation="RVILPAARAAQKQGILASDPLPADTLFGKATKGSFDGVVALYHD
                     QGLIPLKLVAFGTCVNLTVGLPIIRTSVDHGTAFDIVGKGVADPGSLIEAVKLAAKIT
                     QNRTAQNRMAATPIKKGRVRRVA"
BASE COUNT          327 a          403 c          468 g          359 t
ORIGIN      
        1 tccgatcgga cccatccgtc tgcgacttgc cttatgtagg cagttgttat cggtggaggt
       61 tcgagcgagt catcccatat atacggcgag tcagtccgat aaactgctcg atggaaagga
      121 tttccgcacg ggtggatgga gagagattga gggattccaa tgctgctgcg accgacttct
      181 gttcgtaccc ctgatctttt agcgaattca ccaatgtttt acggcggtgc gcaaatgcgg
      241 ctttcaccag cgcggaaaat ttgggctctt cctcttggtt cagtgctctc tgatgcttgg
      301 tctttagcag catgagggca gagtcgactt ccggttttgg cctgaagcac tgcgcggata
      361 cgcggaacgc tttcgtgatc tctgccgcat attgtgccat gactgacagc actccgtagc
      421 cggagcttcc gggtttggcc acgagtcggt cggcgacttc gttttgcagc atcagcacca
      481 tgcggggaaa acggtttcgc tgatcgagaa gccggaagag gagaggtgtg gacaaataat
      541 atggaagatt ggcgacgacg atggtgctga tcggaaggtg ttcgatcgga tagatcatcg
      601 catcgtcgag gacgagcgtg agattcgaaa accgggcctg tcgttcagtc aggtaagcat
      661 ggagtcgcgg atcgacctcg atcgccgtca cgtggccggc agcacgacat agcgcttccg
      721 tgaggatacc gcggccaggc ccgatttcca aaacggtatc ggtcggggtt agctcagtca
      781 cggcgacgat tttacgaaca atgttgggat cgatgagaaa attctgtcca agacgtttga
      841 tcgctgcagg ggggagaggg gagtccaccc gtcaactccc agttggagga gcctgggcca
      901 atcgtttggc taggatcact agtggcgagc gataacattc cacgagatcc gtaaactcct
      961 cgaccatgtc gttggtgaac gacggccccg gttggatacg agctattgcc tgtccttcca
     1021 gaggaccgac ggtcgtggtg atgagcgcca ccgtgcgctc tttccatttg gctacccgtt
     1081 ccgttccggc aacggtgttc agatcttgca gagtctgtct ggcaagcgta tccagttctt
     1141 ctatctgctg cctaaccacg gtgagaccct tcgacatatc aggcgacacg tctgacccgt
     1201 cccttcttga ttggagttgc cgccattcga ttctgagcag tcctattctg agtgatcttg
     1261 gcagccagct tgacggcctc aatcagactt ccgggatcgg ccacgccctt cccgacgata
     1321 tcaaacgctg ttccatgatc cacggaggtg cggatgatag gcaaacccac ggtgagattg
     1381 acgcaggtac cgaaggcgac aagtttcaga ggaatgaggc cctgatcgtg gtagagggcg
     1441 acgacgccgt caaaggaccc tttcgtggct ttcccaaaga gggtgtcagc cgggagagga
     1501 tcacttgcga gaatgccctg tttctgggcc gcacgggcag ctggaagaat cacgcgg
//
LOCUS       DASTWE010000563         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1340184, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000563 DASTWE010000000
VERSION     DASTWE010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340184"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..474
                     /locus_tag="VFT30_05820"
     CDS             25..474
                     /locus_tag="VFT30_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5092181.1"
                     /translation="MKVSKRATYGIMVAVDLAIHANEAPIQARAIARRQGIPVRFLEQ
                     VLHAMKKAGLVESQRGAQGGYLLTHEPATVSIADIFEALEGPVFHRLFVGQQTRDRHA
                     TKPELLLDDVWEQVQQAERKVLENITVEQLAMRLRTIDAQRNPMYHI"
     gene            516..2999
                     /locus_tag="VFT30_05825"
     CDS             516..2999
                     /locus_tag="VFT30_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU5092182.1"
                     /translation="MNSVESLTIPKIVTNPIPPAILEEIETFEVEALRTLAGEISTDL
                     FKPFRLQYGIYGQRQPGVQMVRIKIPFGGITANQLRRVAELADRYATGVGHVTTRQDI
                     QLHFVELKDVPSIMRGLAEVGLTTREACANTVRNVTACHLAGVCQGEIFDVTPYAKTV
                     AYHLLRNPLNQSLPRKFKIAFSGCKHDCALTPIHDIGLLAVKGANGEIGFRMVAGGGL
                     GSAPRVAQLLREFTPMDELIPSIEAVIKVFDTLGNRKNRNKARMKFVIDKLGFDEFKR
                     RWEAAYVSMGHALPKNGPLTLLQHQDEPPRLIMPTRNGVANGNGNGHGTHTIGYETPF
                     EMWRRTNVVKQKQDGYVTAAIKLFMGDITAEQMLFVADLAERYSNGNLRTTINQNLVI
                     RWVPADQIPHFYEDLAAHRLADPGAELVEDIIACPGTDTCGLGITSSKGLARALAEVF
                     PAGRVPEDLQDVNVKISGCHNSCAQHHISTIGLHGVGKRLGEHVAPHYELHLGGSVNG
                     TAKIGQMTVKLPAKAVPAAVSHLIDVYRRDRQANENLPKFIARVGKSKLKDELIPYTI
                     VPSYQEDPTFYYDWEGEEEFILEDLGPGECAGGALEMIENGILEADQELYQAKLLVEK
                     HQYSVSVNKSYRAVLAAAKALLVTEGLEPSTDAETFIEFDSRIAQKGVVSSVYRDLSK
                     KVGDLGPKETSAESAREKMAFAKGFVDACRTATDQMGKDLKLSAVKEEKPPVAPAPVE
                     TKPVAPAPTGAPVYDLRGVACPMNYVKTKLKLEMMDAGEQLEVWLDAGEPIKNVPMSL
                     KNDGHKVLIQEALEPEASHYRILVEKVEG"
     gene            3016..>3482
                     /locus_tag="VFT30_05830"
     CDS             3016..>3482
                     /locus_tag="VFT30_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEU5092183.1"
                     /translation="MTVNGRSELVAELKAWSASFETKQPQDVLVAAIERYGQKIVVAC
                     SFGAEDVVLVDMVHRINPSVPLFYLDTDFLFPETYATRDRVIERYGLQSTQVIQVKSL
                     LTPEVQAESYGEALWARDPDQCCQLRKVEPLTRVLRGYDAWITGIRRDQSPSRA"
BASE COUNT          859 a          878 c         1003 g          742 t
ORIGIN      
        1 taattcctac cggaataata aagaatgaaa gtatccaaac gtgccacgta cgggataatg
       61 gtagccgttg atcttgccat acatgcaaat gaagccccga ttcaagcacg agctattgca
      121 aggcgccagg ggattcccgt ccgttttctc gaacaggttc tccatgcgat gaaaaaggcc
      181 ggcttagtag aaagccaaag gggtgcccaa ggagggtatc ttctcacgca tgaaccggcc
      241 acggtgtcaa tcgccgatat attcgaggcg ttggaagggc cagtctttca tcggttattt
      301 gtcggtcagc agacccgtga tcgtcatgcg accaagccgg agttgctgtt agatgatgtc
      361 tgggagcaag ttcagcaggc ggaacggaaa gttcttgaaa acattactgt ggaacagttg
      421 gcgatgcgtc tacggacgat cgacgctcaa cgcaacccaa tgtaccacat ctgacctaga
      481 aggctcctct cttgaggagg caaggagatc ggtatatgaa ctcagttgaa tccctcacca
      541 tcccaaaaat tgtcaccaat ccgattccgc cggccattct agaagaaata gaaaccttcg
      601 aggttgaggc cctacgaaca ttggctggag agatctctac cgatctcttc aagccgttcc
      661 gcctgcaata cggcatctac ggccagcgtc agccaggggt gcagatggtg cgcatcaaga
      721 tcccgttcgg cgggatcact gcgaatcagc tgcgacgtgt ggcggagctt gccgaccgct
      781 atgcgaccgg cgtcggtcac gtcacgacga gacaagatat tcagctgcac ttcgtcgagc
      841 tgaaggatgt gccgagcatc atgcggggcc tcgcagaagt cggcctcacc accagggagg
      901 cctgcgccaa taccgtgcgg aatgtgacgg cctgtcacct agccggcgtg tgccaaggag
      961 aaatattcga tgtgactccc tatgcgaaga cagttgccta tcatttgttg cggaaccccc
     1021 tgaatcaaag cctgccgcga aagttcaaaa ttgcgttttc cggctgcaag catgactgtg
     1081 cactgactcc tatccacgat attggcttgt tggccgtcaa aggagcgaac ggagagatcg
     1141 gctttcgaat ggtggctggt ggaggcttgg gttccgctcc acgggttgcc caacttctcc
     1201 gtgagttcac accgatggat gagctgattc ccagtatcga ggccgttatt aaagtcttcg
     1261 atacactcgg caatcgaaaa aatcgaaaca aggcccggat gaagttcgtg atcgacaaac
     1321 tgggatttga cgaattcaaa cggcgctggg aagcagccta tgtctccatg ggacacgcac
     1381 ttcccaagaa cggacctttg acgcttcttc agcatcaaga tgaacccccg cggctcatca
     1441 tgcccacgag aaacggtgtg gcaaatggta acggcaacgg tcacggaact cacacaattg
     1501 gctacgagac gccgttcgag atgtggagac ggaccaacgt cgtgaaacag aagcaagatg
     1561 ggtacgtcac ggccgctatc aagctgttca tgggggatat tacggctgaa cagatgctgt
     1621 tcgtcgcaga tctggctgag cgctattcga acggcaatct tcgcaccacg atcaatcaaa
     1681 acctggtcat ccgatgggtt cctgccgatc agatcccgca tttctacgag gacctggcag
     1741 cccatagatt ggcagatccc ggtgccgagc tggtggaaga cattattgct tgccccggta
     1801 cggatacatg tggtcttggg atcacatcat ccaaaggtct tgcacgagcg ctggccgagg
     1861 tgtttccagc aggccgtgtc ccggaagatc ttcaggatgt gaacgtgaag atcagcggct
     1921 gtcataattc ctgcgcccag catcacattt caacaatcgg gttgcatgga gtcggaaaac
     1981 gcctcgggga gcatgtcgcc ccgcactatg aattgcatct cggtggttcg gtgaacggca
     2041 cggccaagat cggccagatg accgtgaagt tgcccgcgaa agccgtgccg gcggcggtct
     2101 ctcatttgat cgacgtgtac cggcgcgatc gccaagcgaa cgaaaatttg ccgaaattca
     2161 tcgcacgcgt cgggaagagc aagctgaaag acgagttgat cccgtacacg atcgtgccgt
     2221 cttatcaaga ggatcccaca ttctattatg attgggaggg tgaagaagaa tttatccttg
     2281 aggatctcgg acccggcgaa tgtgcaggcg gtgcgctgga gatgatcgaa aacggcattc
     2341 tggaggccga tcaagagctc taccaggcga agctgctcgt tgagaagcat cagtattctg
     2401 tatcggtgaa caaatcttat cgagcagtac tggctgcggc caaggctctg ttggtgaccg
     2461 aagggctgga accatcgaca gatgccgaaa cgttcatcga gttcgacagt aggattgcgc
     2521 agaaaggtgt tgtgtcatcc gtctatcgag atctcagcaa gaaagtcggc gatctgggac
     2581 cgaaggagac gtcggctgaa tcggcccggg agaagatggc ctttgccaag gggtttgttg
     2641 atgcctgtcg cacggcgacg gatcagatgg ggaaggacct caagctgtcg gctgtgaaag
     2701 aagaaaaacc tccagttgcg cctgcaccag ttgaaacgaa accagttgct cctgctccga
     2761 cgggtgcgcc tgtttacgac ttgcgtggcg tggcatgccc aatgaactat gtgaagacga
     2821 agttgaagct ggaaatgatg gacgcgggtg aacaattgga agtgtggctc gacgcaggtg
     2881 aaccgatcaa gaatgtgccg atgagcctta agaacgatgg acacaaggta ctgatccaag
     2941 aggcattgga accggaagcg tcacattatc ggattttggt cgaaaaggta gaaggataaa
     3001 aggatctgat ttgccatgac cgtgaatggc cgttccgaac tagttgcaga actcaaagcc
     3061 tggagcgctt cgttcgagac gaagcagccc caagatgtac tggtggctgc tatcgagcga
     3121 tacggccaaa agattgttgt ggcgtgcagc ttcggagcgg aagatgtggt cctcgtcgac
     3181 atggtgcatc ggattaatcc ctcggtgccg ttgttctatc tcgataccga ttttttgttt
     3241 cctgaaacct atgccacgcg ggatcgagtg attgagcgat acggtcttca gtcaacgcag
     3301 gtcatccagg tgaagtcatt gctcacacca gaggtccaag cagaatcgta cggcgaggcg
     3361 ctgtgggctc gagatccgga ccagtgctgc caactgcgga aagtcgaacc gctgacccgc
     3421 gttctgcgcg gatacgacgc atggattacc ggaatcaggc gggatcaatc cccatctcgt
     3481 gc
//
LOCUS       DASTWE010000564         1296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_209148, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000564 DASTWE010000000
VERSION     DASTWE010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209148"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFT30_05835"
     CDS             <1..1089
                     /locus_tag="VFT30_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5092184.1"
                     /translation="PIEAYHIFSEFLSQESKRLRRVILIVDEAQSLGAELLEELRLLS
                     NMNDGKTLKLQIILSGQPDLQTLLQRIDMTQFAQRIVVDYHLKPLSEIDTANCIRHRI
                     RVAGGQPSLFTNKACALVHRLSRGNPRLINQVSDIALTYGYAEQARIITSKLVAQAAL
                     DRSKGGILPLAAKEELVGLAEAQQDPTELDDPILTTVSIESSQAYPVSSPVEDYARAM
                     VLKEEGRLKEAVELFHSAARDKSVWFKAYSQVGLCYVKMKEHHAAIQAFRTALEDPTV
                     LQQDTLDVLYVLGRSLESIGRVGQALEVYHRINYVTPMFRDVANRLRELQQPSKQVSH
                     KGKSVTGKHSWFSGVVGNVQRFLIGSQK"
BASE COUNT          329 a          331 c          330 g          306 t
ORIGIN      
        1 ccgatcgaag cctatcatat cttttcagag tttttgtcgc aggaatcgaa acgtttgcga
       61 cgggtcattc tcatcgttga tgaagcccaa agtcttggag ctgagttgct cgaagaactg
      121 cggttgttgt ccaacatgaa cgacggcaag accttgaagc tgcagatcat cctctcagga
      181 caaccagacc tccagacgct tcttcagcgc atcgacatga ctcaattcgc acaacggatc
      241 gtcgtcgact atcatctgaa accgctctct gaaatcgaca ccgctaactg tattcgccat
      301 cgaatacgag tcgctggtgg acagccgtcc ctctttacga acaaggcctg tgcattggtg
      361 catcgattga gtcgagggaa cccacgactg atcaatcaag tatcagatat cgccttgacc
      421 tacgggtacg ccgaacaagc aagaatcatt acctccaagt tggtggccca agcagccctt
      481 gatcgaagca agggaggaat ccttcccctg gcggcgaaag aggagttggt cggacttgca
      541 gaagcgcagc aagatcccac ggaactggac gatcctattc tcaccacagt gtccatagaa
      601 tcgtcccagg cctatccggt gagctctccg gtggaggact atgcgcgagc gatggtgctt
      661 aaggaagaag gacgattgaa agaggcagtg gagctgtttc attcggcggc cagggacaag
      721 tccgtatggt tcaaggccta ttcacaagtc ggattgtgct acgtcaagat gaaagaacat
      781 catgctgcca tacaggcgtt tcgcactgcc ctggaggatc ccacagtcct gcaacaagat
      841 actcttgatg tgctctatgt attggggcgc agtctcgaat ccatcgggag ggttggccaa
      901 gcgctggaag tctaccatcg catcaattat gtaaccccga tgtttagaga tgtcgcgaat
      961 cggttacgag agttgcagca gccgtcgaag caggtgagtc acaaagggaa atccgtcacg
     1021 ggtaaacact cttggttcag cggtgtggtg ggcaatgtcc aacgctttct catcggcagt
     1081 cagaagtaaa cattcctgta acacccagta tttccatatc tcactgatgt tctgatcgaa
     1141 aggcgcgtga gatcgggcca tcatggaagc gatccgcttc acttggctga cacagcatga
     1201 taaacacagg cacagcgtct tgacgcattc cgtcggctat agatagcttt tcttttcttt
     1261 ctatctaaac ggtacaattt ctgctttgat tcacaa
//
LOCUS       DASTWE010000565         1821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1210739, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000565 DASTWE010000000
VERSION     DASTWE010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210739"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFT30_05840"
     CDS             complement(<1..618)
                     /locus_tag="VFT30_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5092185.1"
                     /translation="MKTMVLDHTNDVSTSPLRLHDRPIPLPKAGQVLVKIHVCGVCRT
                     DLHVVEGELPDIALPLVPGHQAVGTVVQLGAKVLEIKEGDRVGIAWLQGTCGQCEFCT
                     SGRENLCLQASFTGYQVDGGYAEYAAVSARFAYPIPTIFSDEEAAPLLCAGIIGYRAL
                     RLSGIKPGQRLGLYGFGSSAHIAIQIARHWGCQVYVSSLKPEHQAL"
     gene            complement(631..1242)
                     /locus_tag="VFT30_05845"
     CDS             complement(631..1242)
                     /locus_tag="VFT30_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5092186.1"
                     /translation="MKIHSNWMQRIVIASLLGFFPLSVEAETARWDIDPDHSVLEFRV
                     THMVVSKTSGRFLDYRGFVEMDADAKTLKTIEATINAESINTNQEKRDTHLRNADFLD
                     VKQFPTITYKMKTYRKEGDTYKVVGNLTLRGVTKEVPLVGTFNGVTKDPWGNTRAGFT
                     AEGTLNRKDFGMIWNKTLDTGGLVVGDEVQIHLDIECIKTKQS"
     gene            1411..>1821
                     /locus_tag="VFT30_05850"
     CDS             1411..>1821
                     /locus_tag="VFT30_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5092187.1"
                     /translation="MQALVKTTAGAGLTITDWADPTPGPHDAVVKVAATSLCGTDAHI
                     YRWDEWAQRRIHPPRIIGHELCGHVVEVGREVSLVKVGDYVAAESHLTCGACFQCRTG
                     QAHVCKNYKILGIDRDGSFAQYVALPEGVLWHTAP"
BASE COUNT          394 a          502 c          486 g          439 t
ORIGIN      
        1 caacgcttga tgctccggct tgagcgaact gacatagacc tgacaacccc aatggcgtgc
       61 gatctgtatc gcgatgtgtg cggaggaacc aaacccatac aacccgaggc gctggcccgg
      121 cttgataccg ctgagccgta atgctcgata gcctataatg ccggcacaga gcaggggagc
      181 agcttcttca tcagaaaaga tcgttggaat tggataggcg aatcgtgccg acacggcagc
      241 atattcagca taccccccgt cgacctgata gccggtgaac gaagcctgaa gacagaggtt
      301 ttcgcgtcca cttgtgcaga attcgcactg cccgcatgtt ccctgaagcc aggcaatccc
      361 gactcggtcc ccttccttga tctctaagac cttggcgccg agttgcacca ccgtacccac
      421 tgcttgatgg ccgggtacaa gtggcaaagc aatatcggga agttctcctt ccaccacatg
      481 gagatccgtg cgacagacgc cgcagacatg aatcttgacg aggacctgac ctgcctttgg
      541 aagcggtatc ggtcgatcgt gaagccgcaa aggactagtg gacacgtcat tggtatggtc
      601 aagcaccatg gttttcatgc atgccgtagc ctaagattgc ttggtcttaa tgcactcgat
      661 gtccagatgg atctggactt cgtctccgac gactaacccg ccagtatcga gcgtcttgtt
      721 ccaaatcatg ccgaaatcct tgcgattcag tgttccctcg gcggtgaatc ctgctctggt
      781 gttgccccag ggatccttgg tgacgccatt gaaagtcccg accaacggga cctctttggt
      841 cactccgcgc agggtgagat taccgacaac cttataggta tctccctctt ttcgataggt
      901 tttcattttg tacgtgattg tcgggaactg tttgacgtcc aagaagtcgg cgttgcgcag
      961 gtgggtgtcg cgtttctctt ggttcgtgtt gatagattca gcattgatcg tcgcctcaat
     1021 cgtcttcaag gttttcgcgt ccgcgtccat ctcgacaaat ccacgatagt ccaggaaacg
     1081 ccccgacgtt tttgacacca ccatgtgcgt cacacgaaac tcaaggactg aatgatcagg
     1141 atcgatgtcc caacgagccg tttccgcctc gacgctcagc gggaagaatc ccaatagact
     1201 cgctatcacg attcgctgca tccagtttga gtggattttc atgccatcac cttttggctt
     1261 ctccgtgccg tctgtgtgag taaatactta ccttctgggc gatcatcgcg atgagacccg
     1321 gcatcctacc tatcgcgagg aagcccgtgc aacccgtttc tttcaccgaa acacgggtgt
     1381 caactgattg agttggtata gaataggttc atgcaagcac tcgtcaaaac tactgcaggg
     1441 gctggtttaa ccatcaccga ttgggcggac ccgaccccgg ggccgcacga tgcggtggtg
     1501 aaggtagccg cgacctcgtt atgtggaacc gacgcccata tttatcgttg ggacgaatgg
     1561 gcacaacggc gaattcatcc accgcgaatc atcggccatg aattgtgcgg ccacgttgtg
     1621 gaggttggtc gtgaggtttc tctggtcaag gtcggtgact atgtcgccgc cgaatcgcac
     1681 ctgacctgtg gagcctgctt tcaatgtcgc accgggcagg cgcatgtgtg caaaaattac
     1741 aagattcttg ggatcgatcg ggacgggtct ttcgctcagt atgtcgcact gcctgaaggt
     1801 gttttgtggc acacggctcc a
//
LOCUS       DASTWE010000566         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1178562, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000566 DASTWE010000000
VERSION     DASTWE010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178562"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..626
                     /locus_tag="VFT30_05855"
     CDS             51..626
                     /locus_tag="VFT30_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEU5092188.1"
                     /translation="MINTCKDEHVVRYAQVKGPELEEVIASKTDRQWYALQTHSRHEK
                     QVRDRLLAVGIEPLLPLGKQRRQWSDRKVWTMLPLFSGYCFANFTLDTSLAILQTPGV
                     VRIVGTVKPEPIPAEEIAVLQQVSSVNPVMEPCDYLTEGAWVEVIRGPLAGLRGQLVR
                     RGAHHGLVMRASLLQRAALIHIETDDVIPVQ"
     gene            690..971
                     /locus_tag="VFT30_05860"
     CDS             690..971
                     /locus_tag="VFT30_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5092189.1"
                     /translation="MPKLRFTQDQIRVMVSELDAGQMAGEISRRYGISISTLYRWRAK
                     FTDEQQPDDKERLRSLEDEHRRLKKQFAELTLDYATLRAALIRDVRGDC"
     gene            1083..1700
                     /locus_tag="VFT30_05865"
     CDS             1083..1700
                     /locus_tag="VFT30_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEU5092190.1"
                     /translation="MTKAFIGALVLAMGMSVVGCAGPSADVIAEVNSTKKDFLLGPED
                     VLDITVWRSEDLTQKGVVVRPDGNISMPLIGEIEVSGRTAKELAAHIAERLKEYKEKP
                     SVHVKVKEVNSYYVYVLGDVAKPGKYQVKSHATVLQAIAMAGGFTTYAAKNNVQVRRT
                     VVGQDGRSKEFKIPARYDDLLSGDGEIKDFVLKTGDMVVVPGRAW"
     gene            1764..2984
                     /locus_tag="VFT30_05870"
     CDS             1764..2984
                     /locus_tag="VFT30_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092191.1"
                     /translation="MTIQIPNQVQAQSSTAPMSEKKLVAEPDTHIIPSLQVAERYDSN
                     VFFVPGTNLEDYVTTVSPQLKLNHRNQWVEGKVGGGATGEVYAKNSGLNYVGANGTVD
                     LNLDGTINKFIHGVALRVADTISYTPQLQSFATSSADAQGIDSFIRGLQAQRANSFVN
                     IGTVQGSYTFSSSMSFTSIYTDQRLRFGNAIATPSGSVPRGLIDTNFQTVTSGPVVNL
                     SPSDTVSLSHLYQHGTFGDGGTQSSFSIHGATGKWSRSMTPALQATVEGGFAVISSSG
                     NVQSTGAASLRWQGEYTTVLVSYSRAITPSFLVASTALLSQVVTGAVNRQITGPLSLS
                     LSGSYAVNESVPDSSLLRFESYSITPSIYYLIGKTFTTRLSYTHSQFEQTFASQTFPF
                     DRNLVQLSMTAEWK"
BASE COUNT          750 a          741 c          841 g          688 t
ORIGIN      
        1 ggtttaatgg acgtatttat taaagagact ggggctcacg gggcgaagct atgatcaaca
       61 cctgtaaaga tgagcacgtt gtacggtacg cacaggtaaa gggaccggag cttgaagagg
      121 taatagcatc gaaaactgat cgccaatggt atgcccttca aacacattct cgccacgaaa
      181 aacaggttcg ggatcgactg cttgcagttg ggattgaacc cttgctccca ttgggaaagc
      241 aacgtcgcca atggtccgac cgtaaagtat ggacgatgct cccactattt agcggatatt
      301 gttttgctaa tttcacgtta gacacgagtc ttgctattct acagaccccg ggtgttgttc
      361 gcattgtcgg cacggtgaaa ccggaaccta ttccggctga agaaattgcc gtactccaac
      421 aggtttcgtc ggtcaacccc gtgatggaac cctgcgacta ccttactgag ggcgcatggg
      481 tagaagtgat acgcggtccg ctcgccggcc tccgcggcca acttgtacga cggggggctc
      541 accatgggct ggtgatgcgc gcctccctcc tgcagcgagc tgcgctcatc cacatagaaa
      601 ccgacgacgt tatacccgtg cagtgaatct tccttcttcc cctccattct tcctacggaa
      661 atggaatggc aagaggggag ctggcagtca tgcccaaact ccgttttaca caagatcaga
      721 taagagtcat ggtttcggaa ctagacgccg gtcaaatggc cggagagatc tcacgtcggt
      781 atgggatttc cataagtacc ctttaccgat ggcgggcgaa gtttaccgat gagcaacaac
      841 cggatgataa agagcgatta cgttcattag aggatgaaca tcgacggctg aaaaaacaat
      901 ttgccgagct cacgcttgat tatgcgacgc ttcgggcagc cttgatcaga gatgtgaggg
      961 gagactgtta gacggcaagt gttgtcaaag aagcaaacag ttatgggtgt aggtcttcag
     1021 cgaagtaatg aactgggcga ccaggtcgtt gtgccctcgc catgaaaagg aaggagatga
     1081 gcatgaccaa agcgttcatc ggggcgctgg tccttgcgat ggggatgagc gttgtcggtt
     1141 gcgctggtcc ctcggcagac gtcatcgccg aggtcaacag taccaagaag gattttttat
     1201 tgggacctga ggatgtgctc gacattacag tgtggcgcag tgaggatctg acgcagaaag
     1261 gcgtcgtggt aagacctgac ggcaatatct cgatgccgtt gattggagaa atcgaggtga
     1321 gcgggcgaac ggccaaggaa ctagccgcac acattgccga acggctgaaa gagtataaag
     1381 agaagccatc agtgcacgtc aaagtcaaag aggtcaacag ctactatgtg tatgtcttag
     1441 gagacgtcgc gaagccgggc aagtatcaag tcaaatcgca cgcgaccgtt ctacaagcca
     1501 tcgcaatggc aggagggttc accacctatg ccgcgaagaa caatgtgcaa gtgcgccgca
     1561 ctgtggtcgg gcaggacggt cgctccaaag aattcaagat tccagctcgg tatgatgatt
     1621 tgctgtctgg agacggagaa atcaaagact ttgtattgaa gaccggcgac atggtggtgg
     1681 tgcccggcag agcatggtaa agaggcatgg tgtggcaagg ccgtggaatg agaagctgcc
     1741 tcgccctcgt tgctatgtat ggaatgacga tacaaattcc caatcaggtg caagcgcaat
     1801 cctctactgc acccatgtcg gaaaagaaat tggttgccga accggacacg catatcattc
     1861 cgtcgcttca ggtggccgag cggtatgaca gcaacgtctt ctttgtccct gggacgaatc
     1921 ttgaagacta cgtcacgaca gtttcaccgc agctcaaatt gaatcaccgg aatcaatggg
     1981 tggaaggaaa ggtcggcggt ggagcgacgg gggaagtcta tgccaagaac tctgggttga
     2041 attacgtggg tgcgaatgga actgtagatc tgaatttgga tggcacgata aacaagttca
     2101 tacatggggt ggcattgcgt gtggcagata ctatttctta cacaccccag ttgcaatcat
     2161 ttgcaacttc ctccgctgac gcgcaaggca tcgactcctt tatccgaggg ctccaggcgc
     2221 agcgagccaa ctcattcgta aatatcggca cggtgcaagg ttcttacaca ttttcctcca
     2281 gcatgagctt cacttcaatc tataccgacc aacgattgcg atttgggaat gcgattgcta
     2341 cgcctagtgg gtccgttcca agaggcctca tcgataccaa ctttcaaact gtgacatcag
     2401 ggcctgtggt gaacctatcg ccgtccgata ccgtctcgct ctctcatctg taccaacatg
     2461 gaacgttcgg ggatggagga acacaaagtt ctttttccat tcacggggcc actgggaaat
     2521 ggtccagatc gatgactcca gctctccagg ccacggtcga aggagggttt gcagtgataa
     2581 gctcaagcgg taacgtacag tcaacgggtg ctgcttcgtt gcggtggcag ggtgagtaca
     2641 ccaccgtcct ggtgtcctat tcgcgggcca tcacgccgag tttcttggtt gcgtcgacgg
     2701 cattgttgag tcaggtcgtg accggagcag tgaatcgtca gataaccggg cccttgtctt
     2761 tgtcgttgag cggcagttat gccgtgaatg agtctgtacc ggatagttct ctccttcggt
     2821 ttgagtccta ctcaatcacg ccgagcatct attacttgat tggtaagacc ttcacgacca
     2881 ggctgtccta tacccacagc caattcgagc aaacttttgc gtcccagaca tttccatttg
     2941 accggaatct ggtccaactc agcatgacgg cggagtggaa atgagtatga acgaaaccga
     3001 cacgcccgct gtctccaatc
//
LOCUS       DASTWE010000567         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1049576, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000567 DASTWE010000000
VERSION     DASTWE010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049576"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..590
                     /locus_tag="VFT30_05875"
     CDS             144..590
                     /locus_tag="VFT30_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5092192.1"
                     /translation="MHTTVRRAVLDDLDQLAPLFDAYRQFYGQHSDRILARQFLSDRL
                     VRNESVVLIPEDCVGSVVGFAQLYPTFSSILAAPMYVLSDLFVIPEVRRRGVGTLLLQ
                     SAVETARAAGAVRLELATAITNISAQRLYEALGWQKDDFYLYGLSL"
     gene            complement(605..841)
                     /locus_tag="VFT30_05880"
     CDS             complement(605..841)
                     /locus_tag="VFT30_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092193.1"
                     /translation="MLDSHCCVPNCLEKPYAPAGTCSICKDHFLNFLTWRRRRGTQMF
                     HTYAGMTMEERDTIAAEWMKTIQTDELPAPAPKL"
     gene            1069..>1875
                     /locus_tag="VFT30_05885"
     CDS             1069..>1875
                     /locus_tag="VFT30_05885"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU5092194.1"
                     /translation="MGSDFLPQGVAVGDISAQRALLWLRTDGPASVQIEWATVSRWER
                     ASKLATVMAPVSRTASITTIAETDYTLIIPLEDLIPATRYRYHVLVGSADQTTRRLSA
                     SLAAMGEFTTLPDDKTSATVTFAWSGDLGGQGRCRQGMGGYPIFDVIHRHSPDFFLFL
                     GDTIYSDNPCPSPPNEPGADFKATTLQTYRVRHRYQRGAEALRRFLRTVPVYVIWDDH
                     EVRNDFAGPYDEQMPAGRQALREYWPIASPEEDPHRMYRSVRCGADLELFI"
BASE COUNT          385 a          491 c          537 g          462 t
ORIGIN      
        1 caaggacggg agataaacca ggctcagtaa aaggatggat tctcgattgg gaacgcggaa
       61 aatagggaag atgaacccaa cgatcaagta atcgaagtac taccagcaag tcattggcaa
      121 ctcaaggtct tctagctaga ctcatgcaca ccacagtgcg tcgggcagtg ctcgatgacc
      181 tcgatcagct cgctcccctt ttcgatgcct accgccaatt ctatggacag cattcagatc
      241 ggattcttgc ccggcaattt ttgagtgatc gtcttgtgcg caatgaatcg gtggtgctca
      301 tacccgaaga ttgtgttgga agtgtggtcg gctttgcgca gttgtacccg accttttctt
      361 cgattctcgc cgcgccgatg tatgtgctca gcgacttgtt tgtcatacct gaagtcagac
      421 ggagaggtgt tgggacgttg ttgcttcagt cagctgtcga aacggctcgt gccgccggag
      481 cggttcgttt ggaactggcg accgcgatca ccaatatttc agcacaaagg ctctatgaag
      541 cgttggggtg gcagaaagac gacttctacc tgtatggctt atcgttgtag caggagcgac
      601 gaggtcagag tttcggtgca ggggcgggca gttcatctgt ctggatagtt ttcatccatt
      661 cggcggcgat ggtgtcgcgt tcctccatcg tcatgccggc ataggtgtgg aacatctgag
      721 tgccacggcg acggcgccat gtgagaaagt tgaggaaatg atctttgcag atggaacacg
      781 tgccagccgg agcatagggt ttttccaaac aattgggcac gcagcagtga ctatccaaca
      841 tctcggttct cctgcgaact tattcagtat gccgggcggc tgtgccgatt ttcaagcccg
      901 atgtccagtg ttgcttgacc tcttcatcag ctatagggta gaagcgccct gatgcgcgcg
      961 ttctttcggt ttgctggttg ccttgcctta gtctctctaa cagttggctg tgctgctttt
     1021 tcaagtgaga gcgttcgatc cagcagttcg tctgagggaa agcccttcgt gggctcggat
     1081 ttcttgcccc aaggcgttgc ggtcggcgat atcagtgcgc aaagggcctt gctttggctg
     1141 cgtaccgacg gacctgcctc ggttcagatt gaatgggcca cggtctcacg ctgggaacgg
     1201 gcctcgaaac ttgccacagt catggccccg gtgtcgagaa cggcctccat cacaacgatc
     1261 gcggagacgg attataccct gattatccca ctcgaagatc tgattcccgc aacacgctac
     1321 cgatatcatg ttttggtcgg ttctgccgac cagaccacaa ggcgtctctc tgcgagtctt
     1381 gcggctatgg gcgaattcac taccctcccg gatgataaga cctccgccac cgtgacgttt
     1441 gcatggagcg gagacctcgg tggtcaagga cgatgccgcc aggggatggg tggctatccc
     1501 atctttgatg tgatccatcg acacagtccc gacttcttcc tatttctcgg cgatacgatc
     1561 tatagcgaca atccttgtcc ttcgccgccg aacgagccgg gtgccgattt caaagcgacc
     1621 acgttgcaga cctatcgagt gcggcatcgt tatcagcgcg gcgccgaggc gctccggcga
     1681 tttttgagga cagttccggt ttatgtgatc tgggatgatc atgaagtgag gaacgatttt
     1741 gccggcccgt atgacgaaca gatgccggca ggccggcagg cattacgcga atactggccg
     1801 atcgcttctc cggaggagga tccccatcgc atgtatcgga gcgttcgatg cggggccgat
     1861 ctcgaacttt tcata
//
LOCUS       DASTWE010000568         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_79940, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000568 DASTWE010000000
VERSION     DASTWE010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79940"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFT30_05890"
     CDS             complement(<1..212)
                     /locus_tag="VFT30_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5092195.1"
                     /translation="MNPSTSALLTDLYELTMAQAYLEQGMDEPAVFEFFVRKLPAHRN
                     FLVAAGLEQVVDYFSEFHFSQEELAW"
     gene            complement(209..814)
                     /locus_tag="VFT30_05895"
     CDS             complement(209..814)
                     /locus_tag="VFT30_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5092196.1"
                     /translation="MPNFATILMIALTSHDALLLADIQNDFLPGGALGISGGDAIIPI
                     LSNYVRLFQTHSLPIFLTRDWHPPDHCSFEPQGGPWPVHCVAGSPGSLPTSSFATPAS
                     AVIIYKAIDRDQEAYSAFHHTALDRHLRALSVHRLFIGGLATDYCVLNSVKDARMRGY
                     DICLLIDGIKAVNRQPDDGRFAEEEMIRLGAAPVRWEMLGT"
     gene            complement(857..1171)
                     /locus_tag="VFT30_05900"
     CDS             complement(857..1171)
                     /locus_tag="VFT30_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5092197.1"
                     /translation="MAVSGPPSLYAQDYPPDRARGKAVYERHCQSCHGPSGRGDGPAA
                     ASLKVPPANFQRFQSFLKSDEELLRTIEHGVVFSPMHSWRGRLTDGEMQDVVAYIRVL
                     SQ"
     gene            complement(1422..>1713)
                     /locus_tag="VFT30_05905"
     CDS             complement(1422..>1713)
                     /locus_tag="VFT30_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5092198.1"
                     /translation="WPMDAVLAEQMEIHALRKANEFLNETATKLGTWGYQTRIATTLG
                     TPVDGILQEAKAMNPDLIFVGSRGRRGLIRMVLGSVSHALLHQGTYPLMIFG"
BASE COUNT          400 a          458 c          492 g          363 t
ORIGIN      
        1 aaccaggcca gttcttcctg ggagaaatga aactccgaga aatagtccac tacctgttcg
       61 agaccggctg cgactaagaa gttccgatgg gcgggcagtt tgcggacaaa gaattcgaat
      121 acggccggtt cgtccatccc ttgttccagg taggcttggg ccatggtgag ctcatacaga
      181 tccgtcagta gggcgctggt ggaaggattc atgttcccag catctcccat cgaacaggcg
      241 cggctcccag gcggatcatc tcctcttcag cgaaacggcc atcatcaggc tgacgattga
      301 ccgctttgat gccgtctatc aacaaacaga tgtcatagcc tcgcatccga gcatccttga
      361 ccgaattcag cacacaataa tcggtcgcca atccgccgat gaacaggcga tgcacactca
      421 aggcccgtag atgacgatct aaagcggtgt ggtggaaagc agaataggct tcttggtctc
      481 ggtcgatggc tttgtaaatg atgacggcgg aggcgggagt tgcaaacgac gacgtcggca
      541 gagacccggg tgagccggcg acacaatgca caggccaagg acctccttgg ggctcgaacg
      601 agcagtggtc gggtgggtgc caatcacgag tcagaaaaat cggcaggctg tgagtctgaa
      661 agaggcgtac atagttcgat agaatgggaa tgatcgcatc gccgccgctg atccccagcg
      721 cacctcctgg tagaaaatca ttctggatgt cagcgaggag caatgcatca tgcgatgtga
      781 gagcaatcat gagaatggtt gcgaagttgg gcaatctgac gttctgttga tctcgggcat
      841 gattctgtca ttgtgcctat tgcgacaaca cgcgaatgta cgccacgacg tcttgcattt
      901 ctccgtcggt gagccggccg cgccacgaat gcatcggact gaagacgacg ccgtgctcga
      961 tggtccgcag taattcctcg tcggacttca agaacgattg aaatcgctga aaattggccg
     1021 ggggaacttt caacgaagcg gctgcaggac catcgcctcg accgcttggg ccatggcagc
     1081 tttggcaatg ccgctcgtac acggctttcc cgcgtgcacg gtcaggagga tagtcctgag
     1141 cgtagagtga cggcggcccg cttacagcca aaactccgaa gaggcatgct gcaaggagcc
     1201 gacatggtat gaactgccac gaagctgcca gcatgagcta actcttcttt gtggtgtgag
     1261 caatgaatgg gctgaaaagc tgctgcagct ttttgctgcc gcatgaggga catgtcactg
     1321 tcccagcttc atgctccttc aatgtcacca cgatcaacga ttccttcccg caatcgaggc
     1381 acttatagtc gtacatcggc atggcacacc tctttcgaca atcagccaaa aatcatgagt
     1441 ggataagttc cctggtgcaa cagggcgtgt gaaacactgc cgagcaccat gcggatgagg
     1501 ccgcggcgcc cccgggatcc cacgaagata aggtctggat tcatggcctt ggcctcctga
     1561 agaattccat caaccggagt acccagcgtg gtcgcgatgc gggtttgata accccaggtg
     1621 ccgagcttgg ttgccgtctc gttgagaaat tcgtttgcct tgcggagggc atggatttcc
     1681 atctgttcgg ctaagaccgc gtccatcggc cag
//
LOCUS       DASTWE010000569         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_920262, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000569 DASTWE010000000
VERSION     DASTWE010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920262"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /locus_tag="VFT30_05910"
     CDS             complement(<1..2066)
                     /locus_tag="VFT30_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5092199.1"
                     /translation="MARDKALAPFNLAHDPLFRVEIIRCSDTDHVLMLTIHHLAFDAW
                     SEELITSELASLYAAFASGTPPLLPELPIQYADFARWQREWMTSEDFEIQRTYWEQQL
                     RDAPALLTLPLDHPRAAKQTFRGSTETLLLSEPLTQRIHLLCRQHQVTPYMLMLAAFG
                     VVLARYSGQDDVCIGTSIANRSRPETEQLVGFLVNTLVLRIRLEENPRFKELLRRVSD
                     VASSAYAHQDMPFEKLVELLGVQRNLPYTPLFQVLFVMVNMPDTEDFNLPGVRLEPFD
                     FDMKLARFDLTLRISEPNGKQAFQCDLEYNTDLFLQSTIVRMLHHYQTLLESVATRPE
                     ARLSELSMLSDAQRRQLLIEWNEPRRDDFQERCIHELFEAHVRQDYAADAVMYEGRRL
                     SYQELDTKANRLARYLQSLGVRPEVKVGLCLERSLDLIVGMFAVLKAGGVYIPLDPKF
                     PADRLGYMLSDSGTRIVLTQTQWSDLLPETEVEQVCLDRDWPMIAAHPEDAPVSAVRP
                     GNLAYIIYTSGSTGRPKGVAIEHRQLTNYVSGLLERLTLDRDSSFATISTVAADLGNT
                     AIFGALCSGRSLHVLSVDRGFDPNAVAEYMEEHRIDVLKIVPGHLAGLLEASRPERVL
                     PRRCLILGGEAVHQSLVERVRALAPDCEIINHYGPTETTVGVLTHRIGREIDGHSTIP
                     IGRPLL"
BASE COUNT          504 a          583 c          543 g          468 t
ORIGIN      
        1 ttcagcagtg gtcggccgat cggaatggtc gagtgtccgt cgatctcccg tccgattcgg
       61 tgggtcaaca cgccgacggt cgtctcagtt gggccgtaat gattgatgat ttcgcaatcc
      121 ggcgccaatg cgcggacacg ctcaacgaga ctctgatgga cggcttctcc gccaaggatc
      181 agacaacgcc gcggcagaac acgttccggc cgcgaggctt ccaacagtcc ggccaaatga
      241 ccgggaacga tcttcaacac gtcgatccga tgttcttcca tatattcggc gacggcattg
      301 gggtcgaacc cccggtctac cgacaggaca tgcagagacc ggcccgagca aagagcaccg
      361 aaaatcgctg tattgccgag gtccgccgca acggtagaga tcgtcgcaaa actggaatcc
      421 ctgtccaatg tcaatcgttc cagaagtccg gagacataat tggtaagttg cctatgttcg
      481 attgccacgc ccttcgggcg gccggtcgag ccggaggtgt agatgatata agccaggttg
      541 cccggacgaa cagcgctgac gggagcatct tcgggatgag ctgcaatcat cggccagtca
      601 cgatcaagac acacttgttc cacttcggtc tccgggagta gatcgctcca ttgtgtctgg
      661 gtaagcacaa ttcgtgtccc gctatccgac agcatgtacc ccaatcgatc tgccggaaat
      721 tttggatcaa gcgggatata gactcctcca gctttcaaaa ccgcgaacat ccccacaatc
      781 aaatccagcg atcgctccag acagagtccg accttaactt caggcctcac tccgaggctt
      841 tgaagataac gtgcgagccg gttcgctttg gtatccaatt cctgatagct cagcctccga
      901 ccttcataca tcaccgcatc agcggcgtaa tcttgccgga cgtgtgcctc gaacagctca
      961 tggatacatc tctcctggaa atcgtctcta cgaggttcgt tccattcgat cagcagctgt
     1021 cggcgttgtg catcgctcag cattgaaagc tcggataacc gggcctccgg acgcgtagcc
     1081 acgctctcaa gcaaggtttg ataatgatgc aacatccgaa cgattgtgga ttgcagaaag
     1141 aggtcggtgt tgtattccag atcgcactgg aaagcttgct tcccattcgg ctcgctgata
     1201 cgcaaggtca gatcaaaccg cgccaatttc atgtcaaaat cgaatggttc cagtcttacg
     1261 cctggcaaat taaaatcttc tgtatccggc atgttgacca tcacgaagag cacttgaaat
     1321 aatggggtat aggggaggtt gcgttgcaca cccagtaatt ccactaactt ttcgaacggc
     1381 atgtcctgat gagcataggc gctcgaagcg acatcgctta ccctccgcag caactctttg
     1441 aaacgtgggt tttcctcgag acggattcga agcacaaggg tattgaccag aaacccaaca
     1501 agctgttctg tttccggacg cgagcgattg gcaatcgacg ttccgatgca gacatcgtct
     1561 tgtccgctat accgtgccag tactacccca aacgctgcca acatcaacat ataaggcgtc
     1621 acttgatgct gccgacacag caaatggatt cgctgagtga gcggctcgga gagaagaaga
     1681 gtctcggtgc ttcctcgaaa cgtttgtttc gcagcgcgag ggtgatctag cggcagagtc
     1741 agcaatgccg gagcatcacg cagctgctgt tcccaatagg tccgttgaat ctcaaagtct
     1801 tcactggtca tccattcgcg ctgccacctc gcaaagtccg catattgaat tggcaattca
     1861 ggcagcaaag gcggtgtccc acttgcaaaa gctgcataaa gcgacgccag ttcgctcgtg
     1921 atcaactcct ctgaccaggc atcgaaggca agatgatgaa tcgtcaacat caagacatgg
     1981 tctgtgtcgc tgcaacggat gatttccacc ctgaacaatg gatcatgcgc cagattgaat
     2041 ggggcgagtg ccttatccct ggccatggtg ttcaggaggt cttcgataac ggagttcg
//
LOCUS       DASTWE010000570         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1114284, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000570 DASTWE010000000
VERSION     DASTWE010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114284"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFT30_05915"
     CDS             complement(<1..974)
                     /locus_tag="VFT30_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5092200.1"
                     /translation="MNPTSHRPELDLLRRQVADLARELAERDRVLHERTRHLQVAQAL
                     AHLGSWNWDIASGKVEWSDELYRVFGYEPQSREMTFDSFVSAVLPDDHDRVLASIDDA
                     LAGAAPYDMECRIVRPDGEVRTIHCCGEIVRDGSGQPLRMSGTALDITDRKQTELALQ
                     QREAHFRALIEHSSDVITVLNLDGTIRFESPSIERLLGYAQHELNGRSVFEFVHHEDL
                     PGVMEKFRLLIQLPGTPQLTEFRFRHKDGSWRNFEGIGRVIRDPDGRCSVIVNSRDIT
                     ERKRTEAMLRTSQEKLRQALRASGTGLWDWNTETNEVVLSEEWKHQLG"
     gene            complement(1195..1278)
                     /locus_tag="VFT30_05920"
     tRNA            complement(1195..1278)
                     /locus_tag="VFT30_05920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1243..1245),aa:Leu,seq:gag)
     gene            1411..2091
                     /locus_tag="VFT30_05925"
     CDS             1411..2091
                     /locus_tag="VFT30_05925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5092201.1"
                     /translation="MIRTDQQGLSSSIAAFFDVDNTILPGEASEVRFFRWLWRRGIVG
                     WLEARESVSWLMRHLPELSLHPLRERKLYLAGKPSQVIQPLGEEFCREELCPRVSPAA
                     MRMLDEHRRAGHAIIFVTGSIDFLIAPVAEALRADRCFASRLEQQNGLYTGFLVPPLP
                     YGEGKRRLIDRLTHELTLDLSQCYAYGDSPGDLDLLRAVGHPTVVNPIRGMASIARRE
                     NWPIVKWQ"
     gene            2169..2804
                     /gene="queE"
                     /locus_tag="VFT30_05930"
     CDS             2169..2804
                     /gene="queE"
                     /locus_tag="VFT30_05930"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEU5092202.1"
                     /translation="MRITEVFHSVQGESTFAGLPCVFVRLTGCPLRCTWCDTEYAFFG
                     GTDQSIDDILDTVRSYRCQLVEVTGGEPLAQPDTATLLRRLCEDGFTVLLETSGAVDT
                     TIVHPSVRIILDVKCPGSGMMERMHWPNVERLRPQDEVKFVIQDRIDYEWAKSILTRF
                     HLTDRCPVLFSPVFGALDPHLLAEWLLADRLPIRLQLQLHKHIWSPDMRGV"
     gene            2894..>3438
                     /locus_tag="VFT30_05935"
     CDS             2894..>3438
                     /locus_tag="VFT30_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5092203.1"
                     /translation="MNIMRVEAKQARVDTETTDALALLICEGEGLPKEDGVILDRALG
                     GTLRELLQSKEFDGKAGEVVVFHTHRKIPAKRLILVGLGKKHELGLDQIRQAMGHAVK
                     RVRHAKCGAFTVALPSVVPHGASLLDVGQAMTEGAILGSYQFTTYRSDVPTGKEVRAM
                     TILASQKGQLRELSEGIRRGVA"
BASE COUNT          743 a          937 c          948 g          810 t
ORIGIN      
        1 tatcccaact ggtgtttcca ttcctcggaa agcaccactt cgttcgtttc cgtgttccag
       61 tcccatagtc cggtccctga cgcacggagg gcttgtcgga gcttttcttg cgaagtccgc
      121 agcatcgcct ccgtgcgctt ccgctcggta atgtcgcgtg aattcacgat tacactgcag
      181 cgcccatccg ggtcgcggat tactcggcca attccttcga aatttcgcca tgagccgtcc
      241 ttgtgacgaa accgaaactc agtgagctga ggcgtcccgg gcagctggat aagcaaccga
      301 aatttctcca tcacaccagg aagatcctcg tgatgaacaa actcgaatac gctacgtcca
      361 ttcagctcgt gctgagcgta tccgagcaat cgttcgattg atggactttc aaaccggatt
      421 gttccatcca gattgagaac cgtgatgacg tcagaggaat gctcgataag cgcacggaag
      481 tgcgcttcgc gttgctgtag ggcaagttcc gtttgcttgc gatcagtgat gtccaaggcc
      541 gtccctgaca tacgaagcgg ctgaccgctg ccatcacgca cgatctcgcc gcagcaatga
      601 atggtccgta cttcgccgtc gggacgaacg attcggcatt ccatgtcgta tggtgcggcc
      661 cctgccaacg cgtcatcgat cgacgccaac acccggtcgt gatcgtccgg tagcacggcc
      721 gatacaaaac tgtcgaaggt catctctcgt gattgaggct catagccgaa gacccggtag
      781 agttcatccg accattccac cttgccgctt gcgatgtccc aattccaact ccctaggtga
      841 gcaagagctt gtgcgacctg gaggtgacga gtccgttcgt gcagcactcg gtcccgttcg
      901 gcgagttcac gtgcgagatc ggctacttgt ctgcgcagca gatccaattc aggacggtga
      961 ctcgtaggat tcatgatgag gctccttgcc gcgcgacgcc gacagtacct caaacgccag
     1021 ctcaactcac cggagtgacc atgtcacgtc gcgaatgatt aatgatggtg cccagcccgc
     1081 cagttcctat agaaagcacc cacaagagta gattagtgtc ctatgagaat aggtgaaagt
     1141 ctagatccaa atggcccgga gttagctgga acctgcggaa gctgtttaaa tcaatggtgc
     1201 cgaaggcggg acttgaaccc gcacggcttt cgccacacgc ccctcaaacg tgcgtgtctg
     1261 ccattccacc acttcggcca ccgaatgact cgaaacgtcc cacaggacgc ctgactgaag
     1321 ggaggcatta taggaggagg gtaaaattct tgtcaatcaa ccggtcgtcc ggtagaatcg
     1381 acttctaatt ttttcttaaa acgctcttcc atgatacgaa cagaccaaca aggtctctct
     1441 tcttccattg cggcattttt tgatgtcgac aataccattt tacctggcga agcaagcgaa
     1501 gtgaggtttt tccgctggtt gtggcgacgc ggtatcgttg gttggctcga ggctcgtgag
     1561 agtgtgtctt ggctcatgcg acatctcccg gaattgtcgt tgcatccact ccgcgagcgg
     1621 aagctgtact tggctggaaa accttcgcaa gtgattcaac cgttgggtga agagttttgt
     1681 cgcgaggaat tatgtcctcg cgtctctccc gccgccatgc ggatgctcga cgagcatcgc
     1741 cgtgccggcc atgccatcat tttcgtaacg gggtccatcg atttcctgat cgcccccgtt
     1801 gctgaagcgc ttcgagctga tcgatgcttt gcgagtcggt tggagcaaca gaacggcctc
     1861 tataccggtt tccttgtgcc ccctctccct tatggcgagg ggaaacgccg actcatcgat
     1921 cggttgaccc atgaactcac gctggatctg tcccaatgct atgcgtacgg agacagtcct
     1981 ggcgatctgg acctgctccg ggccgtcggc catccgacgg tggtgaatcc gattcgtggc
     2041 atggcttcca tcgcccgacg cgagaattgg ccgattgtca aatggcaatg agctgctgct
     2101 agtgatcgga tcaaccgtct ccgcggcgaa tcatccaatc gcttactcgg taagcccaca
     2161 cactactcat gcgcatcacc gaagtttttc atagcgttca aggtgagtcg acctttgctg
     2221 gcctcccctg tgtgtttgtg cgtctgaccg gttgccctct gcgttgcacc tggtgcgaca
     2281 cggaatatgc cttctttggc ggaacggatc agtccatcga cgacattctc gacacggtgc
     2341 gatcctatcg ttgtcagttg gttgaggtga cgggaggtga accgttagca caaccggata
     2401 cggctacact gctgcgtcgg ttgtgcgagg atggtttcac agtcctcctt gaaacaagtg
     2461 gagcggtgga taccaccata gttcacccgt cggtccgtat tatcctggac gtgaaatgcc
     2521 cgggaagcgg catgatggaa cggatgcatt ggcccaatgt cgaacgtcta agaccgcaag
     2581 acgaagtcaa atttgttatc caggaccgga tcgattacga atgggccaaa agcattctga
     2641 ctcgcttcca cttgactgat cgctgtccgg tcctcttcag tccggtgttc ggtgccttgg
     2701 atccacacct gttggcggaa tggctcctgg cagatcgatt acccattcgt cttcagctgc
     2761 aactgcacaa gcacatctgg tcacccgata tgcggggggt gtgaagagtg ttccagacct
     2821 ccaggagatt gcatagatcc agttgaccat catcggttgg acgcttgctg attgacacat
     2881 taaggagaaa cggatgaaca ttatgcgagt tgaagcgaaa caagcccgcg tcgacacgga
     2941 gaccacagat gcacttgccc ttctgatttg tgaaggggag gggctgccta aggaagatgg
     3001 ggtaattctc gatcgagcgc tcggagggac acttcgggag cttctgcaat caaaggagtt
     3061 tgatggaaag gccggtgagg tggtcgtatt tcatacacac cgaaaaattc cggctaagcg
     3121 gttgattctt gtcggccttg ggaagaaaca tgagctgggt ttggatcaga tccgacaggc
     3181 catgggacat gccgtcaaac gggttcgcca cgccaaatgt ggcgccttta cagttgcgtt
     3241 gccgagcgtt gtgcctcatg gcgcctcgct gctcgacgtt ggccaggcaa tgacggaagg
     3301 agcgatctta ggaagctatc aattcacgac ctatcgcagt gatgtcccaa cgggtaagga
     3361 agtgagggcg atgaccattc tggcttcgca gaaaggtcaa ctgcgcgagt tatctgaagg
     3421 gattcgtcgc ggtgttgc
//
LOCUS       DASTWE010000571         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1340386, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000571 DASTWE010000000
VERSION     DASTWE010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340386"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFT30_05940"
     CDS             <1..777
                     /locus_tag="VFT30_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU5092204.1"
                     /translation="NKIPFYGLAVLCADDEYLTALFPRLVKRYHTYGLHDRDGVAPDF
                     KATDISLRQWGAEFRTYFRGKNLGPFRLAVPGVHNVSNALVAIAIGLELEIPVDLIRK
                     GLAAFTGVERRFHLRGEAGGIMVVDDYGHHPTEVKATLAAAKQGWDRRLVVLFQPHRY
                     SRTRDCIGEFAHAFDHADVLFTTEIYPAGESPIPGVSGAGLAETIRSAGHPAVTFIER
                     KETLPDQVLPHLKPGDLVLTLGAGDIWKAGTGILARLESA"
     gene            784..>1011
                     /locus_tag="VFT30_05945"
     CDS             784..>1011
                     /locus_tag="VFT30_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092205.1"
                     /translation="MKHRVQTDGTKVRAMQAQRRLESAVAGVRGVVRFNAPLREYTSF
                     HIGGPADALVEPVDVEDVVRLAKQAHEQKLPI"
BASE COUNT          208 a          264 c          317 g          222 t
ORIGIN      
        1 aacaaaatcc cattttatgg gttagcggtc ttgtgcgccg atgatgagta tctgaccgca
       61 ctgtttcctc gcttggtcaa acggtatcac acctatgggc ttcacgatcg ggacggagtg
      121 gcaccggatt tcaaggccac cgacatcagt ctgagacaat ggggtgccga gttccggacg
      181 tatttccgtg gaaaaaatct tggccccttc cgattggccg taccgggggt ccacaacgtt
      241 tccaatgcgt tggtggccat tgccatcggt ctcgaactgg agattcctgt agacctgatt
      301 cgcaaagggc tggcggcttt cacgggagtc gaacggaggt ttcacttgcg aggggaagcc
      361 ggcggtatca tggtggttga cgactatggc catcatccta ccgaggtgaa agcgactctg
      421 gctgccgcca agcagggctg ggaccgtcga ttggtggttc tcttccagcc gcatcgatat
      481 agccggacga gggattgcat cggggagttc gcgcatgcct tcgatcacgc cgacgttctg
      541 ttcacgacgg aaatttatcc ggccggtgag tcaccgatcc caggagtttc aggagctggc
      601 ctcgctgaga cgatcaggtc ggcgggccat ccagcggtga ccttcatcga gcgcaaagaa
      661 acgctgccgg atcaggtcct gcctcatcta aaaccgggcg accttgtcct cacgttgggg
      721 gcgggtgata tctggaaggc tggaactggg attcttgcca ggcttgagtc tgcttgatgg
      781 ctcatgaagc accgggtgca aacggatggg acgaaggtac gagcaatgca ggcacagcgc
      841 agattggaat ctgccgtggc gggcgttcgc ggggtggttc gcttcaatgc accacttaga
      901 gaatacacgt cgtttcacat cggtggcccg gccgatgcgt tggtggaacc ggttgacgtg
      961 gaagacgtcg tccgtcttgc caagcaggcg cacgaacaga aactaccaat t
//
LOCUS       DASTWE010000572         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_435541, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000572 DASTWE010000000
VERSION     DASTWE010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435541"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFT30_05950"
     CDS             <1..424
                     /locus_tag="VFT30_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092206.1"
                     /translation="LAMDHHAATDYGNRVLTGRSSSPLSRSTVLRSRDGGTRTIGFPA
                     LQNERQKRVWFDPPRSAICKLDGGAQCADCLVLGVWDSGARLRIEDTSIPAEFDLLFA
                     SGPKPVRRRCKRLSVCGDVMDVEFKRTKPSYVLDTGQE"
     gene            546..>1166
                     /locus_tag="VFT30_05955"
     CDS             546..>1166
                     /locus_tag="VFT30_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5092207.1"
                     /translation="MTFPIPPDEPGRLAALRSLQILDTMPAREFDSLVRLAREFLRVP
                     ISAISLVDEHRQWFKAIEGLDCKETEREIAFCAHTICSAEPLIVRDATRDDRFRGNLL
                     VRESPKIRFYAGIPLQLADGFRLGSLCVMDTKPRTITESELACLRHLSELTIALLCNH
                     ARSLQTNQQAKIIEEQEQRLVRNNKLLESACELGKLGAWERDIATGQ"
BASE COUNT          264 a          319 c          341 g          242 t
ORIGIN      
        1 acttgccatg gatcaccacg cagcaacgga ctatggaaat cgtgtcttaa cagggagaag
       61 cagcagccct ctctcccgct caacagttct tcgatctagg gatgggggaa ctcgaacgat
      121 cggctttcct gctcttcaaa acgagagaca gaagagggtt tggttcgatc ctccacggtc
      181 cgctatctgc aagctcgacg gaggagctca atgcgccgat tgtcttgttc ttggcgtctg
      241 ggacagcggc gcgagactcc gcatcgaaga tacgagcatt cccgccgaat ttgatctgct
      301 ctttgcgtca ggtccaaagc cggtaaggag gcgatgcaag cgcttgtcgg tgtgcggcga
      361 tgtgatggac gtcgagttta agcgaacgaa gccgtcctac gtgctggaca ccggacaaga
      421 atgatgaacg ctcgagcttg catagaagcc cgccgaatga gtgagcaaga aaacgccctg
      481 cggtgagtgg cgaactccgg aatgaccctc atcacaactc tatctgtaac agaaggtgcg
      541 tacccatgac atttccaatt ccgccggacg aacccgggcg attggccgct cttcgttcat
      601 tgcagattct ggacacgatg ccggcccgcg agtttgacag cctcgtgcgc ctggcgcgcg
      661 agtttctccg cgtgcctatc agtgcgatct ctttggtcga tgagcacagg cagtggttca
      721 aggctatcga ggggctcgac tgcaaggaga ccgagcgaga gattgcgttt tgcgcgcaca
      781 cgatctgttc agcagagcct ctgattgtgc gggacgctac cagggacgac cgcttccgcg
      841 ggaatttgct cgtcagggag agcccaaaaa tcagattcta tgcgggcatt ccgctacaac
      901 ttgccgacgg cttccgatta ggcagtctct gcgttatgga caccaagccg cgaacgatca
      961 cggagtcgga gctggcctgt ctccggcatt tgagcgaact aaccatcgct cttctctgca
     1021 atcacgctcg ctcgctgcag acaaatcagc aggccaaaat tatcgaggag caagagcagc
     1081 gtcttgtgag gaacaacaag ttattggagt cggcttgcga gctcggcaag ctcggagcct
     1141 gggaacgcga tatcgcgacg ggacaa
//
LOCUS       DASTWE010000573         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1017521, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000573 DASTWE010000000
VERSION     DASTWE010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017521"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="dnaA"
                     /locus_tag="VFT30_05960"
     CDS             <1..893
                     /gene="dnaA"
                     /locus_tag="VFT30_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5092208.1"
                     /translation="YGGVGLGKTHLMHAVGQYVLDHDRTLKLTYISAERFMNEMINAV
                     RYDRIIDFRERYRTVDVLLVDDVQFIAGKEGTQTEFFHTFNALYDSQKQIVISSDCPP
                     HEIQNLEERLRSRFEWGLIADIQSPDLETRVAILKKKADAEAVPLPDDVAIYIAGRIK
                     SNIRELEGSLIRLIAYASLTGQEISLPLAQDVLRNVLEHGERTVTIEIIQKFVADHYN
                     LKLADLKSRNNSKSVAMPRQVAMYLCKSLTHASLPEIGRSFGGKHHSTVIHSIRKVDD
                     LRKKNADFNNQIDGFLKSFR"
BASE COUNT          254 a          323 c          294 g          245 t
ORIGIN      
        1 tctacggggg agtcgggctt ggcaagacac acctcatgca tgctgtcggg caatatgtgc
       61 tcgaccacga ccgcacgctc aagctcacgt acatctcggc tgaacgcttc atgaacgaga
      121 tgatcaacgc cgtccggtac gatcgcatca tcgacttccg cgagcgctac cgaaccgttg
      181 acgtcctgct cgtcgacgac gtgcagttca tcgccggcaa ggaagggacg cagaccgagt
      241 tttttcacac gttcaacgcc ctgtacgact cgcagaagca gatcgtgatc agcagtgatt
      301 gtcccccgca cgagatccag aacctcgagg aacggctgcg gtcccgcttc gaatggggtc
      361 tcatcgcgga catccagtcg cccgacctcg aaacacgcgt ggcgattttg aagaagaagg
      421 ccgatgcaga ggccgtcccg ctgcccgacg acgtggcgat ctacatcgcg ggacgaatca
      481 agtcgaacat ccgcgagctc gagggctctc tgatccggtt gattgcgtat gcgtcgctca
      541 ccgggcagga gatctcgctg ccgctcgccc aggacgtgct gagaaacgtg ctcgagcacg
      601 gagagagaac ggtcacgatt gaaatcatcc agaagttcgt ggccgaccac tacaatttga
      661 agctcgccga cctgaaatcg cggaacaatt caaaatcggt ggcaatgccc cggcaggtcg
      721 ccatgtatct gtgtaagtca cttacccacg cctcgctgcc tgaaatcggc cgcagtttcg
      781 gcggaaagca tcattcgacc gtgattcatt cgattcgaaa ggtcgacgac cttcgaaaaa
      841 agaatgccga tttcaacaac cagatcgacg gctttcttaa gagttttcgc tgaggttgcc
      901 gtagttgcac agtctcagct gtggatgcct gaggaaaacc tgttcgatgc ttccggccga
      961 gcttgaaagc ccgttcccga ggttcaatca ctcacttttt ttgcacactt taagtcactg
     1021 acaggtaagg ggtaatcggg accgttcgct tttcaacaca ccctctattc ctatatactt
     1081 tctcattctc gaatctctcc ttttcgtagg ggattg
//
LOCUS       DASTWE010000574         1343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_338803, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000574 DASTWE010000000
VERSION     DASTWE010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338803"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFT30_05965"
     CDS             <1..460
                     /locus_tag="VFT30_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5092209.1"
                     /translation="FAFNGNGEMTGAAWVDEGGMLEGPIGITNTHSVGIVRDTIIDWQ
                     AKNDSIYQRWSCPVVTETADGWLNDMNGFHVRPQHVWSALDGAKDGPIAEGNVGGGTG
                     MLCYEFKGGTGTSSRKLPEKLGDGPSVPLCSPTLDGATSSPSQESRSGSI"
     gene            394..879
                     /locus_tag="VFT30_05970"
     CDS             394..879
                     /locus_tag="VFT30_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5092210.1"
                     /translation="MQSNFGRRYQLTVARVPVGQHLKENAPFSGGENPFRQDDGSLII
                     LLATDAPLLPHQLKRLAKRASLGMARTGSLGGNGSGDIFLAFSTTSTGMAGESTANVK
                     YLLNDYIDPLLFASAYASEEAIINSMVAGESMIGHRGMSVEALPHDKLVEIMKQYNRL
                     S"
     gene            925..1326
                     /locus_tag="VFT30_05975"
     CDS             925..1326
                     /locus_tag="VFT30_05975"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5092211.1"
                     /translation="MAVYQELKGHRDLKVFQLAYKLAMKIFHESKSFPRDERFSLTDQ
                     IRRSSRSVAANTAEGFRKRQYPKMFVSKLADSDGEVAETQVWLDFARDCGYMPSERRD
                     ELIKGYEEVGKMLGSMMSLPEKFIPRAQRRE"
BASE COUNT          329 a          349 c          387 g          278 t
ORIGIN      
        1 gtttgccttt aacggcaatg gcgagatgac cggcgccgcc tgggtcgacg agggcggcat
       61 gctggaaggt ccgatcggaa ttacgaacac acactcggtc ggtatcgtgc gcgatacgat
      121 catcgactgg caggcaaaga acgactcgat ctaccagcgc tggtcctgcc cggttgtgac
      181 ggaaaccgcg gacggctggc tcaacgatat gaacgggttc catgtcaggc cgcagcacgt
      241 ctggtccgcc ctcgacggtg caaaggatgg accgattgcg gagggaaatg tcgggggcgg
      301 gaccgggatg ttatgctatg agttcaaagg cggcacaggg acatcctcgc gaaaactacc
      361 tgagaaactg ggggatggac cgtcggtgcc cttgtgcagt ccaactttgg acggcgctac
      421 cagctcaccg tcgcaagagt cccggtcggg cagcatttga aagaaaacgc gccgttctcc
      481 ggcggagaga atccgttccg gcaggatgac ggttcgctga tcatcctcct cgccactgac
      541 gcgcccctgc tcccacacca gctcaaacgg cttgcgaaac gtgcttcgct ggggatggcg
      601 cgcacgggct ccctcggcgg aaacggcagc ggcgatattt tcctggcgtt ttccacgacc
      661 agcacaggta tggcaggaga gtcgaccgcg aatgttaagt atctcctgaa cgattacatc
      721 gatccgcttc tttttgcgtc ggcgtacgcc tctgaggaag cgatcatcaa ttcgatggtc
      781 gccggcgaat ccatgattgg acacagaggg atgtctgtcg aagctttgcc tcacgacaaa
      841 ctggttgaga tcatgaagca gtacaaccgg ttgtcttagg gaattgcagg aggcagtagg
      901 cagaacgcag taaaggtgaa aacaatggct gtatatcagg aacttaaagg tcatcgcgac
      961 ttaaaggttt ttcaacttgc atacaaacta gccatgaaaa ttttccatga aagcaaatcc
     1021 ttccccaggg acgaacgctt ttcactgacc gatcagatac ggcgttcctc cagaagtgtg
     1081 gcagcaaaca cagctgaggg attccgcaaa cgacaatatc ccaaaatgtt cgtgagcaaa
     1141 ctagccgatt cagatggtga ggtcgctgaa acacaagtat ggttggattt tgcccgcgac
     1201 tgtggctata tgccttcgga acgtcgagat gaactgataa aaggctacga agaagtgggg
     1261 aaaatgctcg gatccatgat gtccctgcct gaaaagttca tcccgcgcgc tcaacgccgt
     1321 gaataatatt ctcgcagctg ctt
//
LOCUS       DASTWE010000575         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_403287, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000575 DASTWE010000000
VERSION     DASTWE010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403287"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..>1012
                     /locus_tag="VFT30_05980"
     CDS             83..>1012
                     /locus_tag="VFT30_05980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU5092212.1"
                     /translation="MRDYLEFEKPIREIEEKIEKLSATATNGKSSAQNDIRKLRAKLA
                     QTEHELYKHLTPWQRTQLARHPQRPSTLDYISELTRDFLELHGDRVFGDDRAIVGGFA
                     RFNDRPVMIIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMKMAEKFNRPIITFIDTP
                     GAYPGIGAEERGQAEAIARNLFVMSRLTVPIISVVIGEGGSGGALALGVADRVLMLEH
                     SVYSVISPEGCAAILWDSPEKVPDAASALKMTAHDLLELGVIDTIVSEPLGGAHREPR
                     AVCDLVGKALTNQLFDLLDLPVEQLITQRDQKYR"
BASE COUNT          239 a          276 c          273 g          224 t
ORIGIN      
        1 taggcaaagg ggctctctct ttgctatctt aggggtctca attgggccgg ttggccgatc
       61 gttgcacgga ttgaacagag ctatgcgcga ttacctcgaa tttgaaaagc cgatccgaga
      121 gatcgaagag aagatcgaaa aactttctgc gacggccacc aatggaaaat cgtccgccca
      181 aaatgacatt cggaagcttc gcgcgaagct tgcccaaacc gaacacgaac tctacaaaca
      241 tctgaccccc tggcaacgaa cgcagctggc cagacatccg caacgtccaa gcacactgga
      301 ctatatcagt gagctgacgc gagactttct tgagcttcat ggtgaccgtg tatttggaga
      361 cgatcgggcc attgtgggag gatttgcccg attcaatgac cgccctgtga tgattatcgg
      421 tcatcaaaaa ggcaaaaccc tcaaagagcg catgcaacgg aacttcggca tgcctaaccc
      481 ggaagggtat cggaaggcgc tccgcctcat gaagatggcg gaaaagttca accgtcctat
      541 catcactttt attgatacgc cgggtgccta tcctggcatc ggtgccgagg agcgtgggca
      601 agcggaagcc atcgcccgga atctgtttgt catgtctcga ttgaccgtcc cgatcatttc
      661 cgtggtcatc ggtgaaggag ggagcggagg cgccttagcc ctcggcgtcg ccgaccgagt
      721 cctgatgctg gaacattctg tctattcggt catttcacct gaaggctgtg ccgctattct
      781 atgggacagc ccggaaaagg ttcccgatgc cgcctctgca ttgaagatga ccgcacacga
      841 tcttcttgag ctcggcgtga tcgacacgat tgtttccgaa ccgttgggcg gcgctcatcg
      901 cgaaccacgc gccgtctgcg atctggtcgg aaaggcattg accaaccagc tgttcgatct
      961 gcttgatttg cccgttgaac aactcattac tcagcgggat cagaaatatc gc
//
LOCUS       DASTWE010000576         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_468140, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000576 DASTWE010000000
VERSION     DASTWE010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468140"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /gene="lptC"
                     /locus_tag="VFT30_05985"
     CDS             complement(<1..522)
                     /gene="lptC"
                     /locus_tag="VFT30_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU5092213.1"
                     /translation="MSVLLATFLGYLLFRNADSVPSSRPLAPESIEQADAKIEEFIFT
                     QSKGDVVQWQVQAKQARLFEQDKRAVLRDVAFTFYGTGGDEMTVHGEEGTLDTATKNF
                     RLVNRQTPIVVETRSGYTIYTNHLVWMDQAREIRTDDPVRMVGHGLEVKGQGLLGRME
                     SEEFEVLQDVRVDL"
     gene            complement(721..1152)
                     /gene="rplQ"
                     /locus_tag="VFT30_05990"
     CDS             complement(721..1152)
                     /gene="rplQ"
                     /locus_tag="VFT30_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5092214.1"
                     /translation="MRHRKKGRQLGRQTKHRWALFRNLVTSLLDKERIETTGAKAKEV
                     RGFTDRMITLGKEGTLPARRRALGFLRSKAVVSKLFSDVATRFKDRSGGYTRIIKTRR
                     RIGDAAEMVAIELVSRQELTTKKKSDARTVQPASTETGASA"
     gene            complement(1177..2166)
                     /locus_tag="VFT30_05995"
     CDS             complement(1177..2166)
                     /locus_tag="VFT30_05995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5092215.1"
                     /translation="MKDFQIPMRVEVDKDAHSPIFGRFTTEAFERGFGTTIGNALRRI
                     LLSSLTGAAVTTVKIEGVVHEFSTISGVTEDVTAIILNIKSLRLALHTDKPKTIRLKK
                     KGPGEAKGSDILHDGDVTILTPDLHIATLDKDATLDIEMTVKHGRGYVPAERNKEEGL
                     PIGVIAIDSIFSPIRRVNFHVENARVGRMTDYDKLTMEIWTDGTISPRDALSTGAGIL
                     REHLDIFINPEERSEGKSEAGYEESQREVNKNLSRSVNELELSVRAANCLKNANIKTI
                     ADLVQKSEGEMLRTKNFGKKSLNEIKEILSEMGLSLGTKIEAASPHNGSPKPE"
     gene            complement(2260..2886)
                     /gene="rpsD"
                     /locus_tag="VFT30_06000"
     CDS             complement(2260..2886)
                     /gene="rpsD"
                     /locus_tag="VFT30_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5092216.1"
                     /translation="MAKYRGPVCRLCRREGEKLFLKGTRCMTEKCAVERRSYPPGQHG
                     QGRQRTSDYSLQLREKQKLRRIYGLQECQFRGVFERAERQTGVTGDALLRLLECRLDN
                     VAYRLGFGASRKQARQMVSHGHFTVNGKKITVAGALVKPGDVIEIRERSRDLAAIQTA
                     LESVDSRGIPDWLELDKGAFKGTVRALPAKEQIALPVNEQMVVELYSR"
     gene            complement(2974..>3102)
                     /gene="rpsK"
                     /locus_tag="VFT30_06005"
     CDS             complement(2974..>3102)
                     /gene="rpsK"
                     /locus_tag="VFT30_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5092217.1"
                     /translation="GPGSGRESAIRSLQGAGLRINLIRDVTPIPHNGCRPPKRRRV"
BASE COUNT          699 a          921 c          704 g          778 t
ORIGIN      
        1 cagatccaca cgcacatcct gtagaacctc aaattcttcg gattccattc ggccaagcag
       61 tccttgtccc ttcacctcca gcccatgccc caccatccgc acaggatcat ccgtacgaat
      121 ttctcgtgct tggtccatcc ataccaagtg gttggtataa atcgtatagc cgctccgagt
      181 ctccacgaca atgggcgttt ggcggtttac caacctgaaa ttcttggtcg ccgtatccag
      241 cgttccctct tccccgtgca cggtcatctc atctcctcct gtaccgtaga aggtgaaggc
      301 aacgtcacgc agcaccgcac gcttatcttg ctcaaatagt cgggcctgtt tagcttgcac
      361 ctgccactgg acgacatcac ctttcgattg tgtaaagatg aactcctcga tcttggcgtc
      421 cgcttgttca atcgactctg gagcgagggg tcggctgctc ggcaccgaat ctgcgtttct
      481 gaagagaaga tagccgagaa atgtggctaa tagcacactc aaggtgagaa gagtccgtct
      541 cgctacaagt tcccacatgc tctaggattc tccggtgaca gatgcatggc aacacctggc
      601 acgatcgtag catgcacggc ctgtcaagta aacgtcctcc agggaaccgg aaataaaaag
      661 actcccatgt ggtggcacat gggagtcttt ttattggcag cgactattgg tgactaaaaa
      721 ctatgccgat gctccggtct cggtagatgc tggctgaaca gtacgagcat cggatttttt
      781 ctttgtagtg agctcctgac gagagactaa ctcaatagca accatttcag cagcatcgcc
      841 aatgcggcga cgagttttga tgatcctggt gtaccccccg gatcgatctt tgaatcgtgt
      901 cgccacatcg ctaaacagtt tagacactac agccttgctg cgaagaaatc ctaaggctcg
      961 gcggcgcgca ggcagcgtac cttccttacc aagggtgatc atgcgatcag taaaccctcg
     1021 tacctcttta gccttggccc ctgtggtctc gatacgctct ttgtctaaca acgaggttac
     1081 taagttccga aacaacgccc accgatgttt cgtctgccgt ccaagttgtc gccccttttt
     1141 tctatgtcgc acggtagtcc tcttgattgg aaagcatcat tcaggctttg ggcttccatt
     1201 gtgcggagat gcagcctcta tctttgttcc taacgaaagc cccatttcag aaagaatttc
     1261 tttaatctcg ttgagagatt tcttcccaaa gttttttgtc ctgagcatct caccttccga
     1321 cttttgtaca aggtctgcga tcgttttgat attcgcgttc ttcaggcaat tggccgcgcg
     1381 aacagacagc tccaactcat tcacgctacg agagagattc ttattcactt cccgctgaga
     1441 ttcctcgtaa cctgcttcgc tcttcccttc actgcgctct tccggattga taaaaatatc
     1501 caaatgttca cgcaaaatcc cggccccggt agagagggcg tcgcgagggc tgatggtgcc
     1561 gtcggtccag atctccatcg tcagtttgtc atagtctgtc atacgcccga cccgcgcatt
     1621 ctcgacatgg aaattgactc tcctgatcgg agagaagatg gaatcgatgg caatcacccc
     1681 gatcggcaat ccctcttctt tgttacgctc agccggcacg taaccacggc catgtttcac
     1741 cgtcatctcg atgtccagag tcgcatcctt gtcgagagtg gcaatatgca aatcaggtgt
     1801 cagaatcgtc acgtcaccat cgtgaagaat gtctgacccc tttgcttctc caggcccctt
     1861 ctttttcaac cgtatcgtct tcggtttatc ggtgtggagc gccagccgca aactcttgat
     1921 atttagaatg atcgccgtaa catcttccgt tacaccagaa atcgtcgaaa actcgtgcac
     1981 aaccccttca atcttgacag tggtaaccgc agcccccgtg agcgacgaca gcaaaatgcg
     2041 acggagggca ttaccgattg tggtgccaaa cccccgttca aacgcttccg tggtgaatcg
     2101 cccaaatatg ggagaatgtg catccttgtc gacttccacc cgcattggga tctgaaagtc
     2161 tttcatcgct ttgatcatga cccaccctcc ataccatcga gtgactatga catcgacaga
     2221 taatgccgat atcgaaagaa cgcacctcag atcggcggac tatcgtgagt acaattccac
     2281 caccatctgt tcgttgactg gcaacgcgat ctgctctttg gccggcaatg ctcgcacggt
     2341 gcccttgaat gctcctttgt caagctcaag ccaatcagga atgcctcgac tatcaaccga
     2401 ttccaatgcc gtctggattg cggccaagtc ccgactccgt tcgcgaattt cgatcacatc
     2461 tcccggcttc accagtgccc cagccaccgt gatcttcttg ccgtttaccg tgaagtgacc
     2521 atgactcacc atttgacgag cttgcttgcg cgacgcaccg aaccctaagc gataggcgac
     2581 attgtccaag cgacactcga gcaaccgcaa caaggcatcg cccgtgacgc cggtctgccg
     2641 ttctgcacgc tcgaaaacgc cgcggaattg gcattcctga agcccataga ttcgacgcag
     2701 tttctgtttt tcgcgcaact gcagactata gtcagaagtt cgctgtcgtc cctgcccatg
     2761 ttggccaggc ggataactcc tgcgttccac agcgcatttt tccgtcatac agcgcgtgcc
     2821 cttgagaaag agtttctcac cttctcgccg acacaaccga cagactggac cacgatactt
     2881 tgccactgct tcctcctcgc aaaatgactt actgattcac tgcaaacagg tcatgcactc
     2941 tcgctcagcc acgttgacca tccatgcatg gcatcacaca cgccgccgct tgggaggccg
     3001 acacccatta tgcggaattg gcgtgacatc gcgaatcaga ttgatccgca agccggctcc
     3061 ttgaagtgaa cggatcgctg attctcggcc agaacctggt cc
//
LOCUS       DASTWE010000577         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_823665, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000577 DASTWE010000000
VERSION     DASTWE010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823665"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..>1138
                     /gene="selB"
                     /locus_tag="VFT30_06010"
     CDS             95..>1138
                     /gene="selB"
                     /locus_tag="VFT30_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU5092218.1"
                     /translation="MPHIIGTAGHIDHGKTSLIRALTGQETDRLKEEQERGISIDLGF
                     AYLDLPNGDRAGIVDVPGHERFIRNMLAGAHGIDLVLFTIAADDGVMPQSEEHLDFLH
                     LLGLKKGIFVITKTDLVDAARLAEVREEIEILALDTTLEGSAIIPVSSVSGLGLEQLR
                     QEIAAHLQTYERPSLPGYFRLPVDRAFIMKGHGVVVTGTAIAGTIGEGDTVRVLPGGE
                     ESRVRSIQVHGKATPQAKWGQRVALNLAGLEKSALTRGHVICHPKLTMTTDRFDAFVE
                     IRPGAGRDIENHERVRVHLGTAEVIGKVIILDGREILPPRSSGYCQLVLEESVVALRG
                     DRFVLRNQTAQGTL"
BASE COUNT          245 a          290 c          337 g          266 t
ORIGIN      
        1 aaagaagaca gtgtcgtgac cgtcacggaa acgccattcc cttcccatcg cctttttgcc
       61 aaaataccag tcacactcag gagcgtgggt atacatgccg catattattg gcaccgctgg
      121 acacattgat catggcaaga cctcgctgat ccgagcactc accgggcagg agacggaccg
      181 cttaaaggaa gaacaagagc gcggaatttc tatcgaccta ggttttgcat acctcgatct
      241 tccgaatggc gatcgggctg gtatcgtcga tgttccaggg catgagcggt tcattcgcaa
      301 tatgttggcc ggcgcgcatg ggattgacct tgtactgttc acgatcgcgg cggatgatgg
      361 agtcatgccg cagagcgaag aacacttaga ttttctgcat ctgctcgggc tcaagaaagg
      421 gatcttcgtc atcaccaaga ctgacttagt cgatgctgct cgcttagctg aggtgcgaga
      481 agagatcgag atccttgccc tcgatacaac gctcgaaggg tcggcgatta ttcccgtgtc
      541 ctctgtgtca ggtttgggac ttgagcaact tcgccaagag attgccgctc acttacagac
      601 gtacgaacgt ccttcacttc ccgggtactt tcgactgcca gtcgatcggg cgtttatcat
      661 gaaaggtcat ggtgtggtgg tgaccggcac tgctattgct ggcactattg gtgaggggga
      721 cacggtacgg gttctcccgg ggggcgaaga gtcgcgcgta cggtcgatcc aggtgcacgg
      781 gaaagcgacg ccgcaggcga agtggggtca gcgggtcgcg cttaacctcg cgggtctaga
      841 gaagagtgct ctcacacgcg gacacgtgat ttgtcatccc aaattgacca tgactaccga
      901 tcggtttgat gcttttgtcg aaattcgtcc gggggccggg cgggatatcg aaaaccacga
      961 gcgtgtgcga gtgcatctcg gtacggccga agtcattggc aaagtaatta ttctcgacgg
     1021 tcgcgagatc cttcctccgc gttcaagtgg gtactgtcaa cttgttttgg aagagtcggt
     1081 ggtggcgctt cgtggtgacc gctttgtcct gcgtaatcaa acagcccagg gaacgctc
//
LOCUS       DASTWE010000578         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1017710, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000578 DASTWE010000000
VERSION     DASTWE010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017710"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFT30_06015"
     CDS             <1..594
                     /locus_tag="VFT30_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5092219.1"
                     /translation="TVDQLGWKTPDQVVIPMASGSLLTKIWKGLHELKALGLIDNVRT
                     KINGAQAEGCSPISTAFKAGRDFFKPVKPQTIAKSLAIGNPADGYYALKATAESHGSM
                     DMVTDEEVVEGIQLLAQTEGIFAETAGGVTIGVLKKLVKQGVIKKDEVTVAYVTGNGL
                     KTQEAVIDSVGRPVRIQPSLVDFEKTFKMGKNGGGEA"
     gene            591..863
                     /locus_tag="VFT30_06020"
     CDS             591..863
                     /locus_tag="VFT30_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5092220.1"
                     /translation="MIKVRIPTPLRPLTKGQGEVETKADSVLEMIEALNSTHPGIKDR
                     LCDETGELRRFVNIYVNEEDIRFLKGKDTSLKDGDEVSIVPAIAGG"
     gene            866..1105
                     /locus_tag="VFT30_06025"
     CDS             866..1105
                     /locus_tag="VFT30_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEU5092221.1"
                     /translation="MAHLRFHIRFPEDKIREPIIYQIGREYNVVTDVRRADVRDTTGW
                     ADVELSGDTAEIERAVAGLRTKGCVVDPIELNVVE"
     gene            1108..>1407
                     /locus_tag="VFT30_06030"
     CDS             1108..>1407
                     /locus_tag="VFT30_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU5092222.1"
                     /translation="MDLSESEIQRYSRHIILQDVGGKGQLKLKRAKVLLIGAGGLGSP
                     AGLYLAAAGIGTIGLVDGDVVDLSNLQRQIMHSTATLGQPKVESGRKTLSAINPEI"
BASE COUNT          345 a          365 c          411 g          286 t
ORIGIN      
        1 accgtggacc aactgggctg gaaaacaccg gatcaagtcg tcattccgat ggcttcaggc
       61 tccctcttga ccaagatttg gaagggcctg catgagctga aggcgttggg tcttattgat
      121 aacgttcgca cgaagatcaa cggcgcccaa gcggaaggct gctcaccgat ctcgaccgcg
      181 ttcaaggcgg gacgcgactt cttcaaacct gtgaaaccgc agacgatcgc caaatccctt
      241 gccatcggca accctgccga tgggtactat gcgctcaagg cgaccgcgga gagtcatgga
      301 tccatggaca tggtgacgga tgaagaagtg gtggaaggca tccagctgct ggcccaaacc
      361 gaaggcatct tcgcggaaac agccggaggc gtcacgatcg gcgtgctcaa gaagctcgtc
      421 aagcaagggg taatcaaaaa agatgaggtg acggtggcct atgtcaccgg caacggcctg
      481 aagactcagg aagccgtgat cgattcggtc ggtcgcccgg tacgcattca gcccagcctg
      541 gtggacttcg aaaaaacgtt taaaatggga aagaacggtg gtggtgaagc atgattaaag
      601 ttcgtattcc gactccgctt cgccccctga ccaaaggcca gggcgaggtc gagaccaaag
      661 ccgacagcgt gctggagatg atcgaggcat tgaatagcac acatcccggc attaaagatc
      721 gcctgtgcga tgaaacggga gagctccgtc gcttcgtgaa catttatgtc aacgaagaag
      781 acattcgttt cctcaagggg aaagacacgt ccctcaagga tggcgatgag gtttccatcg
      841 tcccggcgat cgcgggagga taaccatggc acacttgcgc ttccatatcc gttttcctga
      901 agacaagatt cgtgagccga ttatctatca gatcgggcgc gaatataatg tcgtgaccga
      961 tgtccgacga gccgatgttc gcgataccac aggctgggcg gacgtggaac tctccggtga
     1021 tacagctgag attgaacggg ccgtcgccgg actgcgaacc aaaggctgtg tcgtcgatcc
     1081 gattgaactc aacgtggttg aataacaatg gatctgtctg aatcggaaat tcaacgctac
     1141 agtcgacaca tcattcttca ggatgtcggt ggcaagggac aactcaagct caagcgggcc
     1201 aaggtcttgt tgatcggcgc aggtggtctg ggctcaccag ccggtctgta tctcgctgcc
     1261 gccggcattg gtacaatcgg cctggtcgat ggggatgtcg ttgatctctc gaatttgcag
     1321 cgacagatca tgcattcgac tgcgacgctc gggcagccaa aggttgagtc cggccggaaa
     1381 accttgtccg ccattaatcc tgaaatt
//
LOCUS       DASTWE010000579         1491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_952797, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000579 DASTWE010000000
VERSION     DASTWE010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952797"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFT30_06035"
     CDS             complement(<1..257)
                     /locus_tag="VFT30_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5092223.1"
                     /translation="MAPDQQPHSDNRSFLSRYVFSTDHKVIAKQYLSLSLFWMVLGAG
                     SAYLIRWQIAHPDTEAFGWGYIDPNLYNQLVTMHGTIMVFFV"
     gene            complement(303..980)
                     /gene="coxB"
                     /locus_tag="VFT30_06040"
     CDS             complement(303..980)
                     /gene="coxB"
                     /locus_tag="VFT30_06040"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU5092224.1"
                     /translation="MQNWFPENVSTFGGDIDGIFWFVFYITTVWFFITEGLILYFVFR
                     YRRKPGQKAVYAAGDTWAQLAWVLVPMAIVVVLDLLIDIRSAEVWAKVKLHRPPTDFV
                     VHGVGKQFNWEFVYPGADGKFGTDDDIKTDNELHVPVGKPIQVILMSKDVIHSFFLPH
                     FRLKQDAMPGRNIPVWFEATKTGEYEIPCAELCGFGHSGMKGLLVVHTPDEYQKWYQK
                     KSTGSGA"
     gene            complement(1237..>1491)
                     /locus_tag="VFT30_06045"
     CDS             complement(1237..>1491)
                     /locus_tag="VFT30_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5092225.1"
                     /translation="EQTQSHRHRSTSIYHVFRGSGTTQIDGETLEWQQGDSFVVPLWS
                     WHSHANASLKDEAILFSLHDEPILRAFGLYLEEAPLVEVR"
BASE COUNT          347 a          406 c          396 g          342 t
ORIGIN      
        1 acgaagaaga ccatgatcgt gccatgcatg gtgaccaact gattgtagag attcggatcg
       61 atataacccc aaccgaatgc ctcggtgtca ggatgggcga tctgccagcg aatcaagtac
      121 gccgagcccg cccctaaaac catccaaaat aacgacaggc tcaagtactg cttggcgatg
      181 accttgtgat cggtggagaa gacgtaacgg ctgaggaaac ttcgattgtc gctgtggggt
      241 tgttgatctg gtgccatgtc gttcacggtt tttacctctt atgtcgttct tcggtattta
      301 agctaagccc ccgagccggt cgatttcttc tgataccatt tttggtactc gtccggcgtg
      361 tgaaccacca agagcccttt cattcccgaa tggccaaagc cgcaaagttc ggcacaggga
      421 atctcgtact ctccggtctt ggtcgcctca aaccataccg gaatgtttct tcccggcatg
      481 gcatcctgct tgagacgaaa atgcggcagg aagaagctgt ggatcacatc cttggacatc
      541 aagatgacct gaatcggttt gccgaccgga acatgcagtt cgttgtcggt cttgatgtcg
      601 tcgtcggtgc cgaacttgcc gtcggcgccg gggtagacaa attcccagtt gaactgcttg
      661 ccgaccccat ggaccacgaa atcggtgggc ggacggtgca gtttaacctt ggcccagacc
      721 tcggcgctgc ggatgtcaat gagtaagtcg agcacgacca cgatcgccat cggcacaaga
      781 acccaggcca gctgcgccca ggtatcgccc gccgcgtaaa ccgccttctg cccgggtttg
      841 cgccggtaac gaaaaacaaa gtagagaatc aacccttcgg tgataaaaaa ccaaacggtg
      901 gtgatgtaaa agacaaacca aaaaattcca tcgatatcgc cgccgaaggt cgagacattt
      961 tcaggaaacc agttctgcat tcagacctct tattttccca ataggtttgc gcatcgcata
     1021 gtattagctg tccattccca gacactgccg gtcaaaacca ctcaggcgta acttgttttg
     1081 ggctcagcgg tcaagagttg cagcgcatgc ggcccggggg cgccgacttg ccggcctcaa
     1141 ccacgcggta agcgcacggc ctgtcgattc ccggctcgaa aatttgtttc gagttatcat
     1201 gtgtgtcttg gttgtcaaag ggtgactgcg gcgaagtcag cgtacttcaa ccagtggcgc
     1261 ttcttcgaga taaagtccaa aggcgcgcaa tatcggctcg tcatggagcg agaacaaaat
     1321 agcttcgtcc tttaacgatg cgttggcatg gctatgccag ctccacagcg gcacgacgaa
     1381 actatcccct tgctgccatt ccaaagtttc accatcgatc tgagtcgtcc cgctgccgcg
     1441 gaagacgtgg taaatgctgg tgctgcggtg acgatggctt tgggtctgct c
//
LOCUS       DASTWE010000580         1319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_435846, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000580 DASTWE010000000
VERSION     DASTWE010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435846"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VFT30_06050"
     CDS             complement(<1..957)
                     /locus_tag="VFT30_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092226.1"
                     /translation="MRMFVRSAIFLGLFLTTLLNGLYAQRGGDWMTVGNDAQRSSWVR
                     RDAKISPETMRKPGFELVWKMKLKNEARQLVSVTPPVLLDFYISYRGFRSLGFVGGSS
                     GNVTGIDIDLARVEWEKAFPTSVSSNPSIECPGGMTSSVTRPTVAGYPGPGFGGFGRS
                     APAASGVGEPHEGAVTLKEREARLAAPPPPPPSASAASRRIAPASNPYAPTVLYVNAI
                     TADGRFHSMYVSNGEEPNPPIDFLPANANARGLIVFDKTAYVATINGCGSADNGVWAI
                     NLESKKVTKWKSDSGVVGSAGFAVGPDGTLYVANTNGDLVALE"
BASE COUNT          310 a          367 c          360 g          282 t
ORIGIN      
        1 ctccagcgcg accaagtctc cgttggtatt cgcgacatac aaagttccat ccggaccaac
       61 agcaaatccg gcagagccga ccacaccgga atcggacttc catttggtga ctttcttcga
      121 ctccaaattg atcgcccaca cgccattatc tgcgctgccg catccgttga tggtggccac
      181 ataagcggtt ttgtcgaaga caatcaaacc gcgggcgttc gcgttggctg gcagaaaatc
      241 aattggtggg ttaggctcct cgccgttgga gacatacatc gaatgaaatc ttccgtctgc
      301 cgtgatggca ttcacgtaaa ggactgtggg agcgtacggg ttcgacgcag gtgcgatccg
      361 gcgactggct gcagaagctg aaggaggagg tggcggtggg gccgccaaac gggcttcccg
      421 ctccttcaaa gtgaccgcac cctcatgggg ttcaccaacg ccggatgcgg ccggtgcact
      481 gcgtccaaag ccgccaaagc ctggcccagg atatcctgcg acagtggggc gcgtgacact
      541 ggaggtcatt ccacctgggc attcaatcga tggattgctt gaaacgcttg tcggaaatgc
      601 cttttcccat tcgacgcgtg ccaggtcgat atcgatcccg gtgacgttgc ctgaactccc
      661 gccgacaaac ccgagcgacc ggaatccacg gtatgaaata tagaagtcga gtaacaccgg
      721 cggtgtcacc gaaaccagtt gccgagcctc attcttgagc ttcatcttcc acaccagctc
      781 aaagccgggc ttccgcatcg tctcggggga aatcttcgcg tcccttctca cccaagatga
      841 gcgctgggca tcgttcccga cggtcatcca atccccgccc cgctgggcat agagaccatt
      901 caacaatgtt gtaagaaaga gaccaaggaa gattgcggaa cggacgaaca ttcgcataga
      961 tgaaagactc ctgtctggcg aaataccgga agtttagtcg ggtatctgaa cgtctcgaca
     1021 acccatttgc agctgaaaca tctgctattg ttgattggcg atcgccgcta gcgggcggcc
     1081 atcccattga cgaccaggag tgtgtcactc cagagtgtca aacaacttta attttgctgt
     1141 atagtgcggt cttcgaaggg aaaagtaaag ccatcatgat accgcacatc gttggagatg
     1201 ccgacctatg agaagagttc tgattgttgt tctatcgatc ctgatttttg gcagcaacgc
     1261 ctcaccggcc gactggctga cggacggcgg cgatgtgatg cgaaacaact ggcagaaag
//
LOCUS       DASTWE010000581         1365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_952817, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000581 DASTWE010000000
VERSION     DASTWE010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952817"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFT30_06055"
     CDS             complement(<1..1326)
                     /locus_tag="VFT30_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5092227.1"
                     /translation="MMSINNILSPANGKPIAVPSQDMVLGCYWLTKERIGAKGEGKVF
                     GSSEEVRIAFDAREVEEHARIKVRLAGSLVQTTVGRVLLSEILPPGLPFANANKLMTK
                     KEMTKLIDAVYRQTGHRDTVEFLDKIKDIGFAYATRAGLSICIDNMHIPSKKEDFIGK
                     AQREVNEIEKQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGDPAKAESFN
                     PIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTHG
                     ARKGLADTALKTANSGYLTRRLVDIAQDVIINEIDCGTRDGIIVSALVEGGEIIQPLE
                     ERVLGRLAAEDIRDPVTGEIIVSWNEEINEELTKSIVEAGVDRVKIRSVLTCQSPRGV
                     CRACYGRDLARGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKV"
BASE COUNT          298 a          414 c          322 g          331 t
ORIGIN      
        1 gaccttactg gccgtgccgc cgatgtgaaa agtccgcatc gtcagctgtg tgcctggttc
       61 gccgattgac tgcgcggcga tgacaccgac cggttcgcct ttttccacca ggcgccctcg
      121 cgccagatcg cggccgtaac aggctcgaca tacaccacgc ggtgattggc aggtcagtac
      181 ggatcgaatc ttgacacgat cgactccggc ttcaacgatc gacttcgtca actcttcatt
      241 gatttcttca ttccaagaca cgatgatttc ccccgtgacg gggtcacgaa tatcctctgc
      301 cgccaaacga ccaaggacac gctcctccaa cggctggata atttcaccgc cttcgactaa
      361 ggcgctgacg ataatgccgt cgcgtgttcc acaatcgatc tcgttgataa tgacgtcctg
      421 agcgatatcg accaatcgcc gagtcagata acctgaattg gcggttttca gcgccgtgtc
      481 agccagaccc ttacgagcac cgtgagtcga gatgaagtac tgcaacaccg tcaacccttc
      541 acggaaattg gcggtgatcg gcgtttcgat gatctcaccg gatggcttgg ccatcagacc
      601 gcgcataccg cccaactgcc gaatctgttg cgaactacca cgggcaccgg aatcggccat
      661 catgaagatt gggttaaacg attcggcctt cgccggatca cctccggcac cgagctcctt
      721 catcatctcg ttggcgacct gctcagtgac gtgtgcccaa atgtcgatca ccttgttgta
      781 ccgttcgccg ttcgtgatca agccttctga atactgcttc tcgatctcgt tcacttcgcg
      841 ttgtgctttc ccaatgaagt cttccttctt actcgggata tgcatattgt cgatgcaaat
      901 cgacaagccg gctctggtcg cataggcaaa accgatgtct ttgatcttat ccagaaactc
      961 gaccgtgtct ctatggccgg tctggcgata tacggcatca atgagtttcg tcatctcctt
     1021 tttagtcatg agcttgttcg cattagcgaa cggcaagccc ggaggaagga tttcggacag
     1081 taagacacga ccaacggtcg tctgaaccag cgaacccgct aggcgcacct tgattcgcgc
     1141 atgctcttct acttcccttg catcaaaggc tatccggact tcttcagaag atccaaacac
     1201 cttcccttcg ccctttgccc ctatccgctc tttcgtcaac caataacagc ccaacaccat
     1261 gtcttgcgac ggtaccgcaa tcggtttacc gttagccgga gaaagaatgt tgttgatgga
     1321 catcatgaga acccgtgctt caacctgtgc ctcaacggac aacgg
//
LOCUS       DASTWE010000582         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1566895, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000582 DASTWE010000000
VERSION     DASTWE010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566895"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFT30_06060"
     CDS             <1..262
                     /locus_tag="VFT30_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEU5092228.1"
                     /translation="TDYDCWHETEEAVTVEAVLGTLHRNVALAKQILRSAMPSFANPI
                     DCPCHRALDNAILTAPKRIPPAVRKKLAVLIDRALTPKKGAR"
     gene            265..>1042
                     /locus_tag="VFT30_06065"
     CDS             265..>1042
                     /locus_tag="VFT30_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5092229.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEGTEILGGSATYFSTAASFFTSVA
                     LIAVVGEDFPQQHIAFLKSRGIDLTGLERRPGATFRWKGEYTHQLNEAHTLDTQLNVF
                     ETFRPHIPEAYRAPDVLFLGNIHPELQLDVLNKVKRPGLVACDTMNFWINGQREALWK
                     VLEKVDILIINDGEARALGQDSNLVKVAKLVLSRGPKHLIIKRGEYGVLMFNEKQIFG
                     APAFPLEGVRDPTGAGDTFAGGFLGYLAATGNRSPEAMRQA"
BASE COUNT          230 a          279 c          304 g          229 t
ORIGIN      
        1 gaccgattat gactgctggc atgagacaga agaggcggtc acggtggagg cggtgttggg
       61 cacgcttcat cgcaatgtgg ccttggctaa acaaatactc cgctcggcca tgccgtcctt
      121 cgccaatccg atagactgtc cctgtcatcg ggcattggac aatgccattc tgactgcccc
      181 gaaacgaatc cctcctgctg ttcggaagaa gctcgctgta ctcattgacc gtgctctgac
      241 accaaagaaa ggagcccgtt agccatgggg aaactgttag tcgttggatc ggttgcgctc
      301 gacacggtca agaccccatt cggtgaagga accgagattc ttggaggatc ggctacctac
      361 ttctcgacag cggccagctt ctttacctcg gtggcgctta ttgccgtcgt tggagaggac
      421 ttccctcagc agcatatcgc gtttctcaaa agccgaggga ttgatctcac cggcctggaa
      481 aggcgtccag gagcaacttt tcgctggaag ggggaataca cgcaccagct gaacgaagcc
      541 cataccttgg atacgcagct caacgtgttc gaaacgtttc gtcctcacat tcctgaagcc
      601 tatcgcgcgc cggacgtgct gtttctgggg aacatccatc cggagcttca gctcgatgtt
      661 ctgaacaaag tgaaacgtcc cgggttggtg gcctgcgaca cgatgaattt ctggatcaac
      721 ggacagcgcg aagcgctttg gaaggtcttg gagaaggtgg acattctgat catcaacgac
      781 ggcgaggcac gcgcactggg ccaagattcc aatctggtga aagtggcgaa gctcgtgctt
      841 tcacgagggc ccaagcatct catcatcaag cggggcgagt atggcgtgct catgttcaat
      901 gaaaagcaga tttttggtgc gccggctttt ccgttggaag gcgtacgcga tccgaccggc
      961 gccggagata ccttcgccgg aggatttctt ggctatttgg cggcgacggg aaaccggtct
     1021 ccggaggcta tgagacaagc ca
//
LOCUS       DASTWE010000583         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1276159, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000583 DASTWE010000000
VERSION     DASTWE010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276159"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..750
                     /locus_tag="VFT30_06070"
     CDS             49..750
                     /locus_tag="VFT30_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5092230.1"
                     /translation="MPKLNKLYRSSIGRPGMAVLVALSGSILLAVPSDSQAEIYQYID
                     AKGTISLTNVPSDTRYRRIDLHPNRLHPVLSEEELEPVISRFSRQHQLHPALIRAIIK
                     TESDFDPRAVSRAGAIGLMQLMPQTAVKLEVRDLYDPEDNIGGGTKYLRQLLDRFRGN
                     LPLALAAYNAGERVVDRYRTLPPIDETRQYVRKVLRYYRLFLARDLAVTGHVIPSSEY
                     AMPRHAPVSSSQSGE"
     gene            complement(683..1090)
                     /locus_tag="VFT30_06075"
     CDS             complement(683..1090)
                     /locus_tag="VFT30_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092231.1"
                     /translation="MERLRRGRVERLEDAEKVRSSLRRTMWGQVGLVRSRESLVRATA
                     QLARWEQLVSGSFAGRADLEVKNMVQVAHCVAEAALWRENSVGAHYRSDFPGSKRPGW
                     KQHSQLYSGIRTTRRTDSKTQGRGVALRTPKTG"
     gene            complement(1100..>1942)
                     /locus_tag="VFT30_06080"
     CDS             complement(1100..>1942)
                     /locus_tag="VFT30_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5092232.1"
                     /translation="ARGDATGNEMVRVLMAQAARQKRIIRLDYHFTIDLVVEEGRCCG
                     AVVLDEHSGEHFIIPAKAVVLSTGGAGQIFARTTNPPNATGDGMAMAFRAGAELQDME
                     FVQFHPTSLYLPSSPPFLLSEAMRGEGGQLRNNRGETFMQRYHPLGVLAPRDIVARAI
                     WAEMAATRARHVYLDVTHLGSSFVKRRFPTIYATCLRHDIDITEEWIPVSPSAHYMMG
                     GVTTDINGATTLPGLFAAGEVACSGVHGANRLASNSLLEGLVFGMRAGIAAVAWASLR
                     SMPD"
BASE COUNT          422 a          610 c          495 g          415 t
ORIGIN      
        1 gagtctgcat ttccttgaca ggcagtgaca gactgctatc gtttgaccat gccgaaactg
       61 aataaactgt atcgttcttc aatcggccgg cctggcatgg ccgtcctcgt cgcattgagc
      121 ggcagcatct tgctcgctgt cccaagtgat agtcaggcag agatttatca atacatcgat
      181 gcaaaaggga cgatatcgct gaccaacgtt ccttctgaca cgcggtatcg ccggatcgac
      241 cttcacccga atcgactgca ccccgttctc tcagaggaag agttggaacc ggtgatcagt
      301 cgattctccc gacagcatca actccacccc gcgcttatcc gcgccatcat caagaccgaa
      361 tcggatttcg accctcgcgc ggtgtcacgt gcgggagcca tcggattgat gcaattgatg
      421 ccgcaaacgg cagtgaaatt ggaggtccgt gacctctacg atcctgagga caatatcgga
      481 ggaggaacga agtatttacg tcagttgctc gaccgattcc gcggcaatct tccattggcg
      541 ctcgctgcgt ataacgctgg agagcgtgtt gtcgatcgtt accgaacact tccgccgatc
      601 gacgaaaccc gccaatatgt ccgcaaggtt ttgcggtatt atcgactctt cctcgcccgt
      661 gatcttgccg tgaccggcca cgtcatcccg tcttcggagt acgcaatgcc acgccacgcc
      721 cctgtgtctt cgagtcagtc cggcgagtag ttcggatccc cgaatataat tgactatgct
      781 gtttccagcc cggccgtttg gatccgggaa aatcggatcg gtaatgggca ccaacactat
      841 tttctctcca gagcgcggct tccgccacgc aatgcgccac ttgaaccatg ttcttcacct
      901 ccagatcggc tctccccgca aaagatccgg acaccagctg ttcccaccgc gcaagttgag
      961 ccgtggcgcg aacgagcgac tcccgagaac ggacgagacc cacttgtccc cacatagtcc
     1021 gtcgaagcga gctgcgcact ttttcggcat cttccaatcg ttccacccga ccacgcctca
     1081 agcgctccac atggtgagtc taatcgggca tcgaacggag agaggcccag gcgacggcgg
     1141 caatgccggc gcgcatccca aataccaatc cctccagtaa tgaattgctg gccagtcggt
     1201 tggccccgtg cacaccgctg caagccacct cgcccgccgc aaaaagacca ggcaatgtcg
     1261 tggctccgtt gatgtcagtg gtcactcccc ccatcatgta atgggcgctt ggagagaccg
     1321 ggatccattc ttccgtgata tcgatatcat ggcgtaggca ggtcgcatag atcgtcggaa
     1381 atcgccgttt gacaaaactc gatcccaagt gagtcacatc aaggtagaca tgccgcgcac
     1441 gcgtggccgc catttccgcc cagatggccc gcgcgacgat atcccgcgga gccaagacac
     1501 cgagcgggtg ataccgttgc atgaacgtct ctcccctatt attgcgcagc tgacctccct
     1561 ctcctcgcat ggcttccgac aacaaaaacg gcgggctcga aggcagatac agcgacgtcg
     1621 ggtgaaactg gacgaattcc atgtcctgga gttccgctcc cgcacgaaat gccatggcca
     1681 tgccgtcgcc ggtcgcattg ggcggatttg tggtgcgagc aaagatctgt cctgccccgc
     1741 ctgtcgacag gacgacagcc tttgcaggaa tgatgaagtg ttcacccgaa tgttcatcca
     1801 gcactaccgc tccgcaacat cgaccttcct caaccacaag atcgatagtg aagtggtaat
     1861 ccagccggat gattcgcttt tgccgtgcag cctgcgccat taaaacccgt accatttcgt
     1921 ttcctgtcgc atcgcctctc gc
//
LOCUS       DASTWE010000584         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_145261, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000584 DASTWE010000000
VERSION     DASTWE010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145261"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFT30_06085"
     CDS             <1..752
                     /locus_tag="VFT30_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5092233.1"
                     /translation="WGIGVAPAILLCILIAMLLSLTHGLLITRLNMQPFIVTLCGLLF
                     YRGLARFITKDETKGFGTVAGLDFLRYLANGNLFGIPMPFVLLLIIAAITFVVLHRSV
                     YGRYLLATGRNAEAARYAGINTKGIVTAAYVIAGALTAISGIIFAFYTNSVSPANHGN
                     AYELYGIAAAVLGGCSLRGGEGSVVGILIGTALLQVLRNLVNLLGIPSSLDFAVMGAV
                     ILIGVMADQILTDRRNRRQVTLMRESTKTAE"
     gene            757..>1009
                     /locus_tag="VFT30_06090"
     CDS             757..>1009
                     /locus_tag="VFT30_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092234.1"
                     /translation="MSTSGKSHGIWEERWHPLREEWVIVAAHRQDRPWSGETVTKAPR
                     AIPTYVDDCYLCPGNPRVSGAQNPKYDGVFVFDNDHPSVG"
BASE COUNT          214 a          269 c          272 g          254 t
ORIGIN      
        1 agtggggcat cggtgtagca cccgcgattc tcttgtgcat tttgatcgcg atgttgttat
       61 cgctcacaca tggtctattg atcacacgac tcaacatgca gccgttcatc gtgacgctgt
      121 gtggtctact tttctatcgc ggtctcgccc gtttcattac caaggacgag accaagggat
      181 tcggcaccgt cgccggtctc gactttcttc gctacctggc aaacggaaac ttgttcggta
      241 tcccgatgcc gttcgtgctt ctcctgatca tcgctgcgat cacgtttgta gttcttcatc
      301 gatctgttta cggacgctat cttctggcaa caggtcgcaa cgctgaagcc gcgcgctacg
      361 caggcatcaa caccaaaggc atagtgactg cggcatatgt cattgccggc gcactcactg
      421 ccatctcggg aatcatcttc gccttctaca caaactcagt gtcgcctgcg aatcacggca
      481 acgcgtatga actctatgga atcgcggcgg cagtgttggg cggttgttcc ctgagaggtg
      541 gagaaggatc agtcgtcggt atcctcatcg gaacagcgtt actgcaagtg ttgcgtaatc
      601 tcgtcaacct tcttggaatc ccaagttcac tcgacttcgc tgttatgggt gctgtgatct
      661 taatcggtgt gatggctgat cagatcctga ctgaccgtcg caatcgtcgc caggtcacgt
      721 tgatgaggga gtccaccaag accgctgaat agagctttga gtacctcggg gaaaagtcac
      781 ggcatttggg aagagcgttg gcatccactg cgtgaagagt gggtaattgt cgcagctcat
      841 cgacaggatc gaccgtggag tggcgagaca gtcaccaagg ccccaagagc aattccaacc
      901 tacgtcgatg actgttatct gtgcccggga aacccgcgtg tcagtggcgc acagaatccg
      961 aaatacgacg gcgtgtttgt tttcgacaac gatcatccga gtgttggac
//
LOCUS       DASTWE010000585         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_920848, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000585 DASTWE010000000
VERSION     DASTWE010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920848"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFT30_06095"
     CDS             <1..499
                     /locus_tag="VFT30_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5092235.1"
                     /translation="ERGTDVDVVLDLMIHDLDLVLSCNPGPVEEVRAAGLSVRSSTND
                     VANAYIQFESGCVASLIASRVSLKAMRHMRLFQPDGYVSIDFQSRQGVVGCRSVESGR
                     KPMVTVEEFQAGDDEPLKLQLKSFLQAVLTQSCPVVSGEDGTAALEVAHRILSAIRVF
                     RDRNT"
     gene            579..>1113
                     /locus_tag="VFT30_06100"
     CDS             579..>1113
                     /locus_tag="VFT30_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU5092236.1"
                     /translation="MTRILIITGEASGDLHGANLAKALKARDPHVSLAGIGGRAMEAA
                     GVRLVEEMGRFDVIGMVGPFALVSIIRRFITMRRLFRSQQWDTVVFIDHPGLNLRFAY
                     FAKAAGLRVVYYIAPQIWAWAPWRIHWIKQRVDHVLVILPFEKPLYDEAGVRCTFVGH
                     PILDAVEDRYDRRELRGR"
BASE COUNT          226 a          281 c          331 g          275 t
ORIGIN      
        1 cgaacgcggc acagatgtcg atgtcgtcct ggatttgatg attcacgacc tcgacttggt
       61 gttgtcttgc aatcccggtc cggtggaaga agtacgcgcc gccggactgt cggtgcgttc
      121 ttctacgaac gatgttgcca atgcgtatat ccaattcgag agcgggtgtg tcgccagctt
      181 gatcgcaagt cgagtttctt tgaaggcgat gcggcatatg cgtctgttcc aaccggatgg
      241 gtacgtatct atagatttcc aatcccgcca gggagtggtc ggctgccgat cggttgagtc
      301 aggccggaaa ccgatggtga cagttgaaga gtttcaggcc ggtgatgatg aacccctgaa
      361 attacagctg aagtcgttcc tgcaggccgt tcttacgcaa tcatgtccgg tcgtgtcggg
      421 tgaggatggg acggccgccc tagaggtggc gcatcgcata ctatccgcca tcagggtatt
      481 cagagatcgt aatacctaag ggcatctcct atcggctgac cataaatcgt ctctcacggc
      541 catgcgtatc aggccgttat catgtcgtgt agattatcat gacccgcatc ctgatcatta
      601 ccggcgaagc ctccggcgac cttcacggag ccaatctcgc caaagcgctg aaagcacgtg
      661 atccccacgt ctctttagcc ggaatcggtg gccgcgcaat ggaggcggca ggggtgagat
      721 tggttgagga aatggggcga tttgacgtca tcggtatggt cggtcccttc gcattggtgt
      781 cgatcatacg acggtttatc acgatgcggc gtttattccg gtcacaacaa tgggatacgg
      841 tggtgtttat cgatcacccg ggtctcaact tgcgctttgc gtattttgcc aaagctgccg
      901 gcctgagagt ggtgtactat atcgccccac agatttgggc gtgggcgcca tggcggattc
      961 attggatcaa acagcgggtc gatcacgtcc tggtcatact cccttttgag aagcccctct
     1021 acgatgaagc gggtgtacgg tgtacatttg tgggtcatcc catattggat gcggtcgaag
     1081 accgctacga tcggagggag cttcgcggca gat
//
LOCUS       DASTWE010000586         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1599629, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000586 DASTWE010000000
VERSION     DASTWE010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599629"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFT30_06105"
     CDS             <1..989
                     /locus_tag="VFT30_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092237.1"
                     /translation="IHLESQTPGRKIHDNANLTRFLERWGAQWWAVDEDLHFHMDGFK
                     LKRVFRNGCDGGDILVFNGIKDRAAWAHVAATQAAALKQDWQAVRRELSLASDWPDDP
                     EILAWASSVCEHLQESRLRQTFLSRYLALEESPKERLSLIRSLLEQKDLRGADSHLQS
                     LLVSSPNHAEGLLLKGILCMQREQYEQAELAFSSARQEGADRKKCLMGMGMAAMGRAY
                     TQGAWERFLEVLAENPDDAEAIHWLLRAGTAQNRWQELSGQLHRYVTRNPGDLAVRFA
                     FASVLLRDEQIEAARREYETLRTLAPGYDGLDQLGQAIAGKQAVLLTEAANA"
BASE COUNT          277 a          302 c          356 g          253 t
ORIGIN      
        1 tcattcatct cgaaagtcaa actcccggaa gaaaaatcca cgacaatgcg aacctgactc
       61 gtttcttgga acggtggggg gcgcaatggt gggcggtcga cgaagacttg catttccata
      121 tggacggttt caaattgaaa cgagtgtttc gcaacggttg tgatgggggt gacatcttag
      181 tgttcaacgg catcaaggat cgcgcagcct gggcccatgt cgccgccacc caggcagccg
      241 ccttgaagca agactggcaa gctgtgaggc gtgaactgag tctggcgtcg gactggccgg
      301 acgatcctga gatattggcc tgggcgtcat ccgtctgtga acatctacag gaatcgagac
      361 tgcgacaaac cttcctctcc cggtatcttg ccctggaaga atctccgaag gaacgactgt
      421 cgctcatccg ctccttactg gaacaaaaag atcttcgggg tgctgacagt catctgcagt
      481 cactgctcgt ctcttcgccg aatcatgcgg aagggctctt gctcaaaggc attctctgca
      541 tgcaacggga acaatatgag caggcggagc ttgctttttc ttcggcacgg caggagggcg
      601 ccgatcgcaa aaaatgtcta atgggaatgg gaatggcggc aatgggaagg gcttacacac
      661 aaggagcctg ggagcggttt cttgaggtgc ttgccgaaaa ccccgatgat gccgaggcga
      721 tccattggct gctccgtgcc ggcacggcgc aaaaccgatg gcaagagtta agtgggcaat
      781 tgcatcgata tgtgacgaga aatcctggtg acttagccgt gcgatttgcc ttcgccagcg
      841 tcttattgcg ggatgaacag attgaggctg cccgccggga atacgaaacg cttcggacgc
      901 ttgcgccagg ttacgatggg ttggaccagc tcggccaggc gattgcagga aaacaagccg
      961 tgctcctgac agaggctgcg aatgcgtgaa ctgattcgcc acctcgttcc ggttgagaat
     1021 gggaagcccg gacctgaaag agttgctcaa tcggttcaga tcgcctcggt gagagagagg
     1081 gaatgtcacg cgcactagtc atacaattgg cgagactggg agatcttctg caatcgctgc
     1141 cggcgattac ggagatccac gcgcgttatc ccgacatgca attcgatc
//
LOCUS       DASTWE010000587         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_629801, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000587 DASTWE010000000
VERSION     DASTWE010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629801"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFT30_06110"
     CDS             <1..455
                     /locus_tag="VFT30_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092238.1"
                     /translation="DFRTLSHDLFPRNLGEVSLSGPIRDLIGEFKGKGGFEINFFERD
                     VPMKMPSGTMTTIFRLLQESLCNVVKHANATHVAVTLAGTGQGGVELVVMDDGIGFDT
                     ARTSDGQKGMGIVGMRERVRLVGGTVKIISQPNHGTTIVFSIPLPNLR"
     gene            1005..>1762
                     /locus_tag="VFT30_06115"
     CDS             1005..>1762
                     /locus_tag="VFT30_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092239.1"
                     /translation="MLVTRAQNTVSKSRTMSDRTATVSRLEEVVSTSDEFDHVVLQAL
                     PILLDRATGYTKRFLRETGQWNDDIEHEKFALRWGSEYLERFLVCGRTEVPCRPLFLF
                     DSLVAKQHSKPEPFCYHPDLLRPLGRFLDGLVARAVISRDALIALYHHSYGWGPGDVI
                     AVTGLNGLESQRIYKNFRRWRESGWQRTMDEIGLTKSELAELENQQRHRQRFNGEAER
                     LIRVAQAHYRKSEPDHYPCLSRSQWGEMFTQGYG"
BASE COUNT          416 a          412 c          518 g          416 t
ORIGIN      
        1 acgatttccg gaccctctct cacgacttgt tcccccgcaa cctgggagag gtctcccttt
       61 ccgggccaat acgtgatctc atcggagaat tcaaggggaa gggcggcttc gagattaatt
      121 ttttcgaacg agacgttccg atgaagatgc cgtccggcac catgacgaca atctttcgtc
      181 tgctgcagga aagtttgtgc aacgtcgtga aacatgcgaa cgcgacgcat gttgcggtga
      241 cgttggcggg aaccggacaa ggaggagtcg aactcgtcgt gatggatgat ggcatcgggt
      301 tcgatacggc tcgcacttcg gatggacaaa aagggatggg aattgtcggt atgcgggagc
      361 gagtccggct ggtcggggga acggtcaaga ttatcagtca accaaatcac ggtacgacga
      421 ttgttttctc gattccgtta ccgaacttac gttgaagcac accttcatta cctaccgtcc
      481 tgatttaggt ctttccagtg gtgaccgggc cgattgctct gatgcctcgc aatcctaact
      541 aacagacttc taggactcgc aaaggcactg gtaattgccc agggaaatct ggttaattaa
      601 ccgtcgtacc ggtcgtcgaa gtcagctaca gcagatagat tgtagggggg aagaaacagt
      661 ttttcatcat ccatcgagcg gcgtgggtgg cctatgcctc cgtttcattc gcaaacacag
      721 gtcaacatag gttgaggggt gcttaggacc acttggcctg aatgatgctc tcgttaggtt
      781 agtctctcgg aatcacggtg tcgttgagag atattgtgtt gcagggagag tatacattcg
      841 aacgaggtgg ttatgtttcc agcagtgccg taatgattat cgaaatctaa tgtgaattta
      901 cattaaaaac acattaacaa ataaagctca ttgaaacaac ggacaaatcc catatgcgag
      961 agggaatatt tgtctagtgc tcctcgatat cgatggaggg gagaatgttg gtaacaagag
     1021 cgcaaaacac ggtcagtaag agtcggacga tgtctgatcg tacggcaacg gtgagccgcc
     1081 ttgaagaggt ggtatcgacg tcggatgaat tcgatcacgt cgtcttacag gcgctgccga
     1141 ttctgttgga tcgagcgact ggttacacaa aacggttcct acgggagacc ggccagtgga
     1201 acgatgatat cgagcatgag aagtttgcgt tgcgttgggg gtccgaatat ctcgaacggt
     1261 ttctggtctg tggacgcacc gaagttccat gtcggccgtt gtttctgttc gactcactgg
     1321 tggccaaaca gcacagcaaa ccggagccgt tttgctatca cccggatttg ctgaggccgc
     1381 tcggtcgatt tctcgacgga ctcgtggcgc gggcggtcat tagccgtgac gcgctgatcg
     1441 cgctctatca ccactcctac ggatggggtc caggggacgt gatcgcggtg accgggctca
     1501 atgggttgga gagccagcgc atttataaaa attttcgacg ttggcgggag agcggctggc
     1561 agcgcacgat ggatgagata ggactgacaa agtcggagct cgcggagctt gagaatcagc
     1621 agcgtcatcg gcagcgattc aacggcgagg ctgaacggct catccgagtc gctcaggctc
     1681 attaccgcaa aagcgagccg gaccattatc cctgtctctc ccggtctcaa tggggggaga
     1741 tgtttacgca aggctatggc tg
//
LOCUS       DASTWE010000588         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1243945, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000588 DASTWE010000000
VERSION     DASTWE010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243945"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFT30_06120"
     CDS             <1..276
                     /locus_tag="VFT30_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092240.1"
                     /translation="FYLPFAVYVGGLLFWSYTVVKLLMIGRLAGYGIGLMFIAGYALL
                     FSNLTLMVVLGVMLLALDRVKPTVAESVPTAAGPVMPASDGLLPGQA"
     gene            295..1101
                     /locus_tag="VFT30_06125"
     CDS             295..1101
                     /locus_tag="VFT30_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5092241.1"
                     /translation="MENPESTLPQSETVLDSGDQPLPPDEEIAGIEKLLADEPDDFQA
                     RCRLGELYFSKGRLDDALVEVKKAIEMAESLRTEMNRSLAMYYANLGTIYATKNMTDE
                     AEAEFRHALDVFAHDVLALFNLGRLYADKKQYMEAKNYYERLVELTPEDPIAWYNLAG
                     VYIELDNPQVSDYNTIDMGIQCYLRTLELDPKHLESSFKLMEIALNHKKTDLAVRVME
                     SAVEHNPDEPLAYYNLISVYDKCKMFEQAEETRKRLKERFAKKPKDGSKS"
     gene            1126..1401
                     /gene="tatA"
                     /locus_tag="VFT30_06130"
     CDS             1126..1401
                     /gene="tatA"
                     /locus_tag="VFT30_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5092242.1"
                     /translation="MFGSLGFTELILILMIVLIIFGAGKLPQLGEGLGKAIKGFKKSV
                     HEADAIEADAQAAAQQAAAPQAVTAASTESAALNQPAGATAQPAPRA"
     gene            1414..>2608
                     /locus_tag="VFT30_06135"
     CDS             1414..>2608
                     /locus_tag="VFT30_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092243.1"
                     /translation="METELGLAAGYIETFAGNGKARSTGDGKRAVKAGIPLPHHLALD
                     KTEQWLYFAESGSDRVRRINLQEGTLHNFAGIGETCYSGDEGLCGEAGLYLPLGVAFD
                     SQNNLYICDSGSNRIRKVDHVTGVITTVVGTGQHGFNGDGPALEVNLTWPAAIAFDHE
                     DVLFIADTQAHKVRRFDPKTGLVTTIAGTWSAEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDQGWLMPVCSDGLDMSMYLDDGRPAMEARLYDIVGLAVDARGDVYVVDKGSNRVRK
                     IDCRTGIISTVAGVCRYGYDGDDKPAVRAMLHAPEAVIFDQNDNLYISDTMNHRIRKV
                     DMKTGLITTVSGNGDSGYEDKNIGGCGAARFVAKESAGMLKHGDGLLGTEAVVNSPVG
                     LAVDSQ"
BASE COUNT          601 a          656 c          767 g          584 t
ORIGIN      
        1 ttctacctcc cctttgcggt gtatgtcggg ggattgctct tctggtctta caccgttgtc
       61 aagttgctga tgatcgggcg actggcgggc tatggaatcg gtctgatgtt catcgccggt
      121 tatgccttat tattctcgaa ccttacgttg atggttgtgc tcggagtcat gttgcttgcc
      181 cttgatcgag tgaaaccaac cgtcgccgaa tcggtgccga ccgccgctgg ccctgtgatg
      241 ccggcatcgg acggcctact tccagggcag gcctagtttc agcgcgagat tattatggaa
      301 aatccagaat cgacgttacc gcaatctgag accgttcttg actccggcga tcaaccgctc
      361 cctcctgacg aggagatcgc cggaatcgaa aaattattgg ccgatgagcc tgacgatttt
      421 caggctcgct gtcgactcgg tgaattgtac tttagcaaag ggcggctcga tgatgctttg
      481 gtagaagtca aaaaagcgat tgagatggcg gaatctcttc gcacggaaat gaatcgctcg
      541 ctggccatgt attatgcaaa tctgggaacg atctatgcga ccaagaacat gaccgatgaa
      601 gctgaagccg agtttcggca tgcgctggat gttttcgccc atgatgtcct ggcgctgttc
      661 aaccttggga ggctctacgc agacaagaag caatacatgg aggcgaaaaa ctactatgag
      721 cgcctcgtcg aactcactcc ggaggacccg atcgcttggt ataaccttgc cggggtctat
      781 attgagctgg ataaccctca agtgtccgac tacaacacca tcgacatggg tattcagtgc
      841 taccttcgca ccttggagct ggatcccaag cacttggaat ccagcttcaa gctgatggaa
      901 atcgcgctca atcataagaa gaccgatttg gcggtcaggg ttatggaaag tgccgtggag
      961 cacaacccgg atgaaccgct tgcgtattac aacctcatca gcgtgtatga caagtgcaag
     1021 atgtttgaac aggctgaaga gacccgcaaa cggttgaaag agcggtttgc caagaagccg
     1081 aaggatggtt ccaagtccta attcactttt aggaagaggt gcactatgtt tggcagtcta
     1141 ggattcaccg agctgattct tatactcatg atcgtgctta ttatcttcgg cgccgggaaa
     1201 ttaccgcaat tgggtgaggg gctcggcaag gcgatcaagg gattcaagaa gtccgtccat
     1261 gaggctgatg cgatcgaggc cgatgctcag gcggcggcgc aacaggccgc tgcgcctcaa
     1321 gccgtgaccg cggcttctac cgagagtgct gcattgaatc aacccgctgg ggccaccgcg
     1381 cagccggctc ctcgagccta gcacgccacg catatggaaa ctgaacttgg tttggccgct
     1441 ggctacattg aaacctttgc cgggaacggt aaggctcgaa gcacgggtga cggcaaaagg
     1501 gcggtaaagg ccggaatccc gcttcctcat cacctcgcac tcgacaagac agagcagtgg
     1561 ctctactttg ccgagtccgg gtctgaccga gtccggcgca tcaatctcca agaaggcact
     1621 ctccacaact tcgccggaat cggggagact tgctacagcg gcgatgaagg tctctgtggc
     1681 gaagcaggac tctatttgcc gttgggcgtc gcgtttgatt cccagaacaa tttgtatatc
     1741 tgtgattccg gaagcaaccg gattcgaaaa gtcgatcatg taacgggcgt cattacgacg
     1801 gtggtcggca ccggtcagca tggattcaac ggggacggtc ccgcgctgga agtcaatctc
     1861 acctggcccg cggcgatcgc ctttgatcac gaagatgttc tgtttatcgc cgatactcaa
     1921 gcgcacaaag tcaggcggtt tgatcccaag acaggattgg tcacgacaat tgcagggacc
     1981 tggtcggccg aagatgaagc gcgagaacag ccgttggtcg cgaggaattt agtggttctg
     2041 tcaggcgacg cgatcggcat cgatttcagc gatgatcagg gatggctcat gccggtctgt
     2101 tcggatgggc tggatatgtc gatgtatctc gatgatggaa ggcccgcgat ggaggcgcgc
     2161 ctctatgaca ttgtcgggct agcggttgat gccaggggcg atgtgtacgt tgtggacaaa
     2221 ggcagcaatc gcgtcagaaa gatcgactgc cggacgggaa ttatttcgac tgtcgcggga
     2281 gtctgccggt acggatacga cggtgacgat aagccggccg tcagagccat gttgcacgcc
     2341 ccagaagccg tgatctttga tcaaaatgac aacctctaca tttccgatac catgaatcat
     2401 cggattcgca aggtggatat gaagaccggc ctcattacca cagtctcggg aaacggggac
     2461 agtggatatg aagacaagaa catcgggggc tgcggggctg ctcgatttgt cgccaaggaa
     2521 tcggcaggga tgttaaaaca tggagacggt ctgctcggga ccgaggccgt agtgaattcg
     2581 cccgtcggtt tggccgtgga ttcgcaag
//
LOCUS       DASTWE010000589         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_759669, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000589 DASTWE010000000
VERSION     DASTWE010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759669"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>1094
                     /locus_tag="VFT30_06140"
     CDS             29..>1094
                     /locus_tag="VFT30_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5092244.1"
                     /translation="MNLHKKTIDDVQLRGKRVIIRADFNVPLDESLQITDDTRIRSTL
                     PTINRVVDEGAKVILCSHLGRPKGTFDPKYSLAPIAKRLGRLLGKDVVFAPDCIGPAV
                     EKLVAKMKDGDVLLLENLRFHAGEEKNDDGFANALASLGDVFINDAFGAAHRAHASTV
                     GITRYIKDAAAGALLKKEIEYLEGAVANPVRPFAAILGGAKVSGKIGVIENLGKKVDK
                     VIIGGGMAFTFLKAKGMEIGNSLCEMDMLDFARGIEDHALSRGVKFYLPVDCVVAASR
                     ELGAETKIVPVQEIPKGWYALDIGPASVKLFNEAVQNAKTILWNGPMGVFEIDAYARG
                     TLAMAHAIADAYALTIVGGGE"
BASE COUNT          263 a          293 c          309 g          229 t
ORIGIN      
        1 gtccatacca accctggagg agcgccccat gaatttgcac aagaaaacca tcgacgatgt
       61 gcaacttcgt ggcaagcggg tcatcatccg cgccgatttc aacgtcccgc tcgatgaatc
      121 gcttcaaatt accgatgaca cccgcatccg ctcaactctg ccgacgatca atcgcgtcgt
      181 cgacgaggga gcgaaagtca ttctctgctc tcacctcggt cggcccaagg gaaccttcga
      241 ccctaaatac agcctggcac ctattgcaaa acgactggga cgcctcctcg ggaaagacgt
      301 ggtcttcgct ccggactgca taggcccggc cgtcgagaaa ttggtcgcta aaatgaaaga
      361 cggagatgtc ctcttgctgg aaaatcttcg ttttcatgcc ggcgaggaaa aaaacgacga
      421 tggctttgcg aatgctcttg cctcgctagg cgatgtgttc atcaatgacg ccttcggggc
      481 agcccaccgg gcccatgcgt cgaccgtcgg catcacgcgg tacataaaag atgcggcagc
      541 aggcgcactg ctcaagaaag agatcgaata tctcgaaggt gccgtcgcca atcccgttcg
      601 accgtttgcc gcgattctgg gaggagcaaa ggtgtccggc aagatcggtg tgatcgaaaa
      661 cctgggcaag aaagtcgata aagtcatcat cggtggcggc atggcgttta ccttcttgaa
      721 ggcgaaaggc atggagatcg gcaattctct ctgcgaaatg gacatgttgg attttgccag
      781 aggaatcgag gaccatgccc tgtcacgcgg ggtcaaattt tacctgcccg tcgattgcgt
      841 ggttgcggcc agccgagagt tgggcgctga aacaaaaatt gtcccggtac aggagattcc
      901 taagggatgg tatgccctcg atattggacc ggcttccgtc aagctgttca acgaggcggt
      961 gcaaaatgcg aaaaccatct tgtggaacgg accaatgggt gtgtttgaaa ttgacgccta
     1021 tgccagaggt accttggcaa tggcccacgc aatcgccgat gcgtacgcgc tcacaatcgt
     1081 tggtggcggt gaga
//
LOCUS       DASTWE010000590         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_209971, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000590 DASTWE010000000
VERSION     DASTWE010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209971"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFT30_06145"
     CDS             complement(<1..303)
                     /locus_tag="VFT30_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5092245.1"
                     /translation="MTVMTGAMHSCKVEDSVALVTLNHPPVNALTPELLTELNTTFDS
                     LAMDDVVKAVVLTGAGRFFVAGADIRVLASIPSSVEGEAMAHQGQIILNKIEAFEKP"
     gene            complement(342..1565)
                     /locus_tag="VFT30_06150"
     CDS             complement(342..1565)
                     /locus_tag="VFT30_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEU5092246.1"
                     /translation="MYIYKVGIVGAGTMGAQIAEVVSYAGLPVVLADVNETLAKRGVD
                     TVRAIYQARVDKGKMSAEQLEEKMLLVAASGGLTGLKDVDVVVEAVSEDAKLKAQVFH
                     ELDQICPRGTILASNTSALSVSALGAATTRPDKVVGLHFFNPAYVMRLVEVVPGLATT
                     PQTLDDAVNFTESIGKLPIVVKECAGFLVNRVLMAYVNEAVRALQEGTATVQEIDQEM
                     VSFGMPVGPLALLDVVGLDISFEVARILYRSYGPRMTPAPLLEAMLKAGRLGVKSGHG
                     FYDYVSGEEGGRDQGLERLLHAFRPNADHAATRWSRSRLLLAMTNEAVIALQEGVASA
                     ADIDLAMMAGIGYPKEKEGPLHFADRLGIDQVLRELEDFAETLGPRFWPAPMLRRMVD
                     AGFTGQMAGRGFFTY"
BASE COUNT          366 a          500 c          421 g          362 t
ORIGIN      
        1 tggtttttca aaagcctcaa tcttgttcaa aatgatctgc ccctgatggg ccatcgcttc
       61 gccctcaacg gatgacggaa tagaggccag tactcggata tcagcaccgg cgacaaaaaa
      121 tcggccggcc ccggtgagga cgacggcctt cacaacgtca tccattgcaa gtgagtcgaa
      181 tgtggtgttc aactctgtga gtaattcagg tgtgagggcg ttgacgggcg gatgattgag
      241 cgttacgagg gcgacgctat cttcaacctt gcaactgtgc atggctcctg tcatgacagt
      301 catgatgcat tccttccttg ccctattcgg agactgagac ttcagtacgt gaagaagcct
      361 cgtccagcca tctgccccgt gaatccggca tccaccattc gccgcaacat cggtgcaggc
      421 cagaaacgtg gtcccaacgt ctcggcaaaa tcttcgagtt cgcgcagaac ctgatcaatg
      481 cccagccgat ccgcgaaatg caacggacct tccttttcct tgggatagcc gatcccagcc
      541 atcattgcca gatcaatgtc tgctgcggag gcgactcctt cttggagcgc aatgacagcc
      601 tcattcgtca tcgccaggag gagccgcgat cgactccagc gtgtcgcagc atgatcggcg
      661 ttcggtctga atgcgtggag aagccgttca agcccttgat cgcgaccgcc ttcctctccg
      721 ctgacatagt cgtagaagcc atggcccgat ttgactccaa ggcgacccgc cttgagcatg
      781 gcttcaagca ggggagccgg tgtcatgcgt ggtccatagc ttcggtacaa gatgcgtgcg
      841 acctcgaacg agatatcaag acccactaca tccagaaggg cgaggggacc gaccggcatg
      901 ccgaaggaca ccatctcttg gtcgatctcc tgcacggtgg cagttccttc ctgcaaggca
      961 cgaacggctt cattcacata ggccatcagg acgcgattga ctaaaaaccc cgcgcattct
     1021 ttcaccacga tcggcagctt cccgatgctt tcggtaaaat ttaccgcgtc gtcgagggtt
     1081 tgtggggtgg tcgcgagtcc aggcaccacc tccacgagcc gcatcacata ggctggattg
     1141 aagaagtgga gtccgacgac tttatccggc cttgtcgtcg cagcaccaag agccgagaca
     1201 gacagtgccg atgtgttact ggctaagatg gtgcctcgcg ggcagatttg gtccaactcg
     1261 tgaaacacct gagccttgag ttttgcatct tcggataccg cttcgactac cacatcgacg
     1321 tccttgagcc cagtcaaacc accagaagcg gcgacaagca acatcttttc ctcaagctgc
     1381 tcggcgctca ttttcccctt gtcgacccgt gcctgataga tcgctcgtac ggtatcaacc
     1441 ccccgtttcg ccaaggtctc attcacatcg gccaacacga caggaagtcc ggcataactc
     1501 accacttccg cgatctgtgc gcccatcgtc cccgcgccga cgattccgac tttatagatg
     1561 tacatgtcgc actctccgtt cttcgtctct gcccatcgtc ataagctcca cccaatcgag
     1621 gaatggcgag aacagaggta caattcaca
//
LOCUS       DASTWE010000591         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_80672, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000591 DASTWE010000000
VERSION     DASTWE010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80672"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1052)
                     /locus_tag="VFT30_06155"
     CDS             complement(<1..>1052)
                     /locus_tag="VFT30_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEU5092247.1"
                     /translation="ELSLLEFNRRVLDLAKDQKVPLLERLKYLCIVSSNLDEFFEVRV
                     AGLKEQVTHGVEQRGADGLTPSELLARIATLTHHLVQEQYVVLNQSLIPKLADQRIRF
                     LKRADWMPSHIRWMRRFFSRELLPLLSPVGLDPAHPFPKLLNKSLNFLVTLEGTDAFG
                     RPNGTAIVQVPRSLPRVIHLPVRHAAWPHDFAFLSSVIHAFVHQLFPGMHVTGCYQFR
                     VTRNSNLFVDDEDVDDLRRALEGQLPDRRFGDAVRLEVADNCPPDMVYFLREQFHLDS
                     HDVYQCHGPVNLHRLMTVPDLVERPDLKYQPFTPGIPTPPIPSDDWFDAIRQGDILLH
                     HPYQSFAPVTEFLRQAA"
BASE COUNT          239 a          275 c          331 g          207 t
ORIGIN      
        1 gcagcctggc gaaggaattc cgttaccggc gcgaaggact ggtagggatg atgcaagaga
       61 atgtcgccct gacggatggc atcgaaccag tcgtcgctcg gtattggagg tgtgggaata
      121 ccgggagtaa agggttggta cttcaggtcc ggccgttcga cgaggtccgg tacggtcatg
      181 agccggtgca ggttgaccgg tccatggcat tgatacacgt cgtgcgaatc gaggtgaaat
      241 tgttcgcgta gaaaatacac catatcgggc gggcagttat ccgccacctc gaggcggacc
      301 gcgtcgccga accgacggtc aggcagttgg ccctcaaggg cccggcggag atcatcgacg
      361 tcttcgtcgt ccacaaaaag attgctgttg cgggtcactc gaaactggta acagccggtc
      421 acgtgcatgc cgggaaagag ttgatgcacg aaggcatgaa tcaccgacga taggaacgca
      481 aaatcatgcg gccaggccgc atggcgtaca gggaggtgga tcacgcgcgg cagcgacctc
      541 ggaacttgca cgatggctgt tccattgggg cgtccgaatg cgtcagttcc ttcaagggtc
      601 accagaaaat tcagactctt attgagcaat ttgggaaacg gatgcgcggg atccaacccg
      661 accggactga gcagtgggag caactcgcgc gaaaagaacc ggcgcatcca gcgaatgtgc
      721 gatggcatcc agtcggcccg cttcagaaag cggatgcgtt ggtcggcgag tttcggaatg
      781 agcgactggt tcagcaccac gtattgttcc tgcaccagat ggtgggtgag cgtggcgatg
      841 cgagccaaca gttccgacgg tgtcagaccg tccgcccccc gttgctctac tccatgcgtg
      901 acttgttctt tgaggccggc gactcgaacc tcgaaaaatt catcgagatt ggagctcacg
      961 atgcagagat attttaatcg ctccagcagg gggaccttct ggtccttggc cagatcgagc
     1021 acccgacggt tgaattccaa caagctcaat tc
//
LOCUS       DASTWE010000592         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1276432, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000592 DASTWE010000000
VERSION     DASTWE010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276432"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFT30_06160"
     CDS             complement(<1..273)
                     /locus_tag="VFT30_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092248.1"
                     /translation="MPRGGSFVCGSLLLLVGITTIANAGEPSDPETAIRQMVRANAEK
                     DLSTLSRLMAHDADIVSYSVGGRKYVGWPEFEREMREEFINTQKIEI"
     gene            complement(356..1111)
                     /locus_tag="VFT30_06165"
     CDS             complement(356..1111)
                     /locus_tag="VFT30_06165"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU5092249.1"
                     /translation="MSTWVGVGRSEIGLVRSLNQDAFAVIDEVSVWAVADGMGGHVGG
                     EVAAQTAITTVQAEAAASSHLLRNGQVSPTDVLTDLISRAHDAILNRARSKSKLKGMG
                     TTIVLLAIVSDPAPVAYLAHVGDSRAYRFRSGTLTLLTKDHTLIEKYLERGILTAETA
                     KTHPERHVLTRALGVGPTVKPTITAFPILPEDLVLLCSDGLTKMLEDEDVRTVFADGE
                     LDPTQVCNRLVAAALDRGGEDNVTVVVVAPRSS"
     gene            complement(1108..>1618)
                     /locus_tag="VFT30_06170"
     CDS             complement(1108..>1618)
                     /locus_tag="VFT30_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU5092250.1"
                     /translation="GASPESGEVTVVGLHREGKRTTLVRGENLTTERLQAELDHCTLL
                     VTFFGSVFDVPVPCAKFPGLLFPTLHFDLCVAARRLALRGELRYVEQEMGIDRNTTIR
                     GLDGLDAVRLWFQWRRASTIARDILMSYNRAATENLVRHADRFYKDDMVSRFRPSSLS
                     TTLQPAYSQ"
BASE COUNT          358 a          467 c          465 g          328 t
ORIGIN      
        1 aatctcaatc ttctgggtgt tgatgaattc ctcgcgcatt tcccgttcga actccggcca
       61 accgacgtat ttccgaccac ccactgagta actgacgata tcggcatcgt gagccatgag
      121 gcgggatagc gtcgagagat ctttttccgc attggcccgg accatttgac gaatagccgt
      181 ttccgggtcg gagggttcac cggcgtttgc aatcgtcgta atgccgacga gaagaagcag
      241 gcttccacag acgaacgatc ccccacgggg catacgggtc tccatgtacg tgacaaagtt
      301 caaatgagaa gggaaactac acggcctgac ggaccatgtc aacaacgaag taaaatcagg
      361 acgaacgagg agcgaccacc accacggtaa cgttgtcctc gccgccgcga tcgagcgccg
      421 cggcgacgag acggttgcag acttgagtcg ggtcgagctc gccgtcagcg aacacggttc
      481 ggacatcctc gtcttccaac atcttggtca gtccatccga gcagagcagt accaggtcct
      541 ccggcagaat gggaaaagcc gtgatggtag gtttcaccgt tgggcctacc cccaaggccc
      601 gcgtcaacac atgccgttcc ggatgagttt tcgctgtttc tgcggtgagg atcccgcgct
      661 ctaggtattt ttcgatcaac gtatggtctt tggtcagaag ggtcagtgtg cctgatcgaa
      721 accgataggc acgactatcg ccgacatggg cgaggtaggc gactggcgca ggatcggaga
      781 cgatcgctag caacacgatg gtcgtgccca tgcctttcag cttggatttt gatcgagccc
      841 gattgagaat ggcgtcatgc gctcggctga tcaggtccgt caacacatct gtgggagaca
      901 cctgaccgtt acggagcagg tggcttgatg ctgcagcctc ggcctgtaca gtggtgatgg
      961 ccgtctgagc ggcgacttcg ccaccgacat gaccacccat tccgtccgcg acggcccaga
     1021 cactcacctc atcgatgacg gcgaatgcat cctggttgag gctgcgcacg agtccgatct
     1081 cgctccgacc gacaccgacc caggtgctca ttgggaatag gccggttgaa gggtagtcga
     1141 caatgaggaa ggcctgaacc gtgacaccat atcatcctta tagaagcggt cggcgtggcg
     1201 cacaagattt tcggtagccg cccgattata gctcatcagg atgtcacggg cgatcgtgct
     1261 agctcgtcgc cactggaacc acagacggac cgcgtccaat ccgtcgaggc ctctgatggt
     1321 cgtgttgcgg tcgatcccca tctcttgctc gacgtacctc agctctccac gtaacgcaag
     1381 ccggcgggcg gcaacacaga ggtcgaagtg caatgttgga aacaggaggc cgggaaactt
     1441 agcacagggg acggggacat caaagactga tccgaaaaac gtcacgagca gggtacagtg
     1501 atcaagttct gcctgcaacc gctccgttgt caagttttca ccacgaacca gggttgtcct
     1561 ttttccctcg cgatgaaggc ctacgacagt cacctctccc gattcgggcg atgcgccc
//
LOCUS       DASTWE010000593         1232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1018139, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000593 DASTWE010000000
VERSION     DASTWE010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018139"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFT30_06175"
     CDS             <1..228
                     /locus_tag="VFT30_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092251.1"
                     /translation="VNIERRLQQHVNDLKFTEEEIATVKTYPLRGTVRDLRIAEQNMI
                     NQMGGKDELLNARNEIAEIFWIDFGVQRFGF"
     gene            228..1073
                     /locus_tag="VFT30_06180"
     CDS             228..1073
                     /locus_tag="VFT30_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092252.1"
                     /translation="MIEVVEMGGPDGPFALIRSDPPTDEEWRMIGERGLRVHVVGLAP
                     GLGLEVFLPHVDKFDQFSIIAGEQADISPIGDMTQLRKLTITGPVRMPIDISRMDNLV
                     SYGGSLRAAPGVLSKSSIRRLSVPFTEDLLRIGAPIKVLRITSARHMRRLPEFSAPHE
                     LIQLTIADAKELSLRGLSDLPSLEELWFDACGLDHADEIARLANLRSLRLEDCRRVEP
                     ASSLAKIVGANVRVVGKHVFSREFRSEVTTSGKAAWSFPSAESAYVQSVGNSRRLPTN
                     RSPKA"
BASE COUNT          267 a          346 c          369 g          250 t
ORIGIN      
        1 gtcaatattg aaagacggct ccaacagcat gtgaatgatc tgaagtttac cgaagaagag
       61 atagcgaccg tgaagacata tccgctcagg gggacagttc gggatctcag aatcgctgaa
      121 cagaatatga tcaaccaaat gggcggcaag gatgaactgt tgaacgcgcg aaacgaaata
      181 gcggagatct tctggattga tttcggtgtt caaaggtttg gattctgatg atcgaggtcg
      241 tagagatggg cggcccggat gggccgttcg cactcattcg ttcggatcca ccaaccgatg
      301 aagagtggcg aatgatcgga gaacgtggcc tacgcgttca cgtcgtggga ctcgccccag
      361 ggctagggct cgaagtcttt ctgccgcacg tagacaagtt tgaccagttc tcgattatcg
      421 ctggtgaaca ggcggacatc tctccgatcg gcgacatgac tcaactgaga aagctcacga
      481 tcacgggtcc ggtgagaatg ccgatcgaca tatctcgaat ggacaatctc gtttcgtacg
      541 ggggttcact ccgcgccgcc ccgggtgttc tctcaaagtc atcgattcgg cggctatccg
      601 tgccattcac tgaggaccta cttcgcatcg gcgcccccat caaggtcctt aggattacct
      661 cggcgcgcca catgaggcgg ttgccggagt tctccgcccc acatgaattg atacagctga
      721 cgatcgcaga cgcgaaggag ctgagcctca ggggactttc cgacctcccc agcctcgaag
      781 agttgtggtt tgacgcgtgc gggcttgacc atgcggatga gattgcacgg ctcgcgaatc
      841 tacgttcact gcgacttgag gactgccgac gcgtcgaacc ggcatcctct ctcgcgaaga
      901 tcgttggcgc gaatgtgcgg gtcgtaggca aacacgtctt ctcgcgggag ttcaggtccg
      961 aagtcacgac atctgggaag gccgcctggt cgttccccag cgctgagtcc gcctacgtcc
     1021 agagcgtggg gaatagccga cggctcccga cgaatcgttc tccaaaggcg tgaggaccgg
     1081 ccggcaaccc gatcctcgac aacgttcgcg tcagggatcc ggacccggac cagcatccta
     1141 ggcctacctg ccagcacgcc ccttcggtgt gccgcaacgc tcatgtgcga ggctttcgga
     1201 ggcagggtac acacctatgg gacgcgtggc gg
//
LOCUS       DASTWE010000594         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1567308, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000594 DASTWE010000000
VERSION     DASTWE010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567308"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VFT30_06185"
     CDS             complement(<1..1107)
                     /locus_tag="VFT30_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5092253.1"
                     /translation="MEKILVVDDEQSLRDVLSIMLKRAGYAVTSAMDGEEAIEFLNKE
                     IFDLVITDLRMPKLDGMEVLKAVKSASPETVVLIITAFATADSAVEAMKQGAYDYLTK
                     PFQVDEVQLIIRNALEKRRLTTENILLKREMASQSSFAQLVGQSEAMQKVFDVVRKVA
                     DSKSNVLICGESGTGKELVARAIHYNSARSVLPFVAVNCSAVPETLLESELFGHMKGS
                     FTGAISNKAGLFEIANGGTIFLDEIGDTTPTIQVKLLRVIQEREFRRVGGNQDVKVDV
                     RVVAATNKDLEQAVAEGSFREDLYYRLDVIPIRLPPLRMRTSDIPLLVNHFLERFSKE
                     SGKAKPVINPEAMHILLGHEWRGNVRELENLIERV"
     gene            complement(1092..2786)
                     /locus_tag="VFT30_06190"
     CDS             complement(1092..2786)
                     /locus_tag="VFT30_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092254.1"
                     /translation="MGDIKARIYWLMGWRVVLVTLLLGLSLAFEVTKGERVETFYALI
                     VFTYAVTILYSFLFQRLTTPQVLVQFAWAQIAVDFLLETVLIARTGGIESPFAVLYVI
                     SVTVASLVPRRRVGLLTASLCIILFGIVTNVQLYGLAEVWGWLPHADLSAAETLHAFG
                     VYGLAFLVVGFLSGALADQLCQADQSLREKEQGLSRLQAFHENIVHSIGSGVFTTDEK
                     GQITSFNPAAQEATGYSFGQVQGRPWREVFNWHPDQQNDGHRLDVSANVRFEVECKQS
                     GGNRLILGMTLAPLHERGERTGLVGVFKDLTQIRDLEEEMRRKEWMASLGEMSAGMAH
                     EIRNPLGALAGAMQMLRKDLEADETSQRLIEIAVREATRLDAIITEFLQYARPPALNL
                     AEYDLNKVLAETLDLVQHEARSRTNITIKAAPCTGALPAQVDQDQMKQVFWNLAVNAF
                     DAMPKGGQLTIATGCRKVDVVGRKAEVVEVSFQDAGEGIPKKNLDKIFLPFFTTKKRG
                     SGLGLAAVHRIVDLHGGWIKVESQEGQGTRFGVCLPRAADSGVRLWHEGREPWKRS"
     gene            complement(2988..4202)
                     /locus_tag="VFT30_06195"
     CDS             complement(2988..4202)
                     /locus_tag="VFT30_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5092255.1"
                     /translation="MATFAYIGRTKSGGVKKGELIAKSRDEAVEQLRKQSVVVTSLEE
                     KAAKGGFSFKLGSGVSEKDLVVFTRQFGTMINAGLPLIQCLEILSTQSENAALRKSVG
                     EIKNQVEGGSTFSDALRKHPKIFDDLYVNMVHAGEVGGLLDTILGRLSKHIEKAMKLK
                     GQIKSALVYPAAIVGIAGIVITVLMIWVIPVFEKMFKEMSNGKMALPGPTQLVIDMSN
                     FAQGNWYIILGGIVASVVAVKKYYATPQGKLALDKLLLKLPVFGDLIRKASVAKFTRT
                     LGTLLASGVPLLEALTICAKTSGNKVVEGALLDAKVSISGGKTIAEPLAKSGTFPKMV
                     THMISVGESTGALDSMLGKIADFYEDEVDQAVTNLTALLEPMMMVFLGVTVGFIVVAM
                     YLPIFTMASAIG"
     gene            complement(4349..>4563)
                     /locus_tag="VFT30_06200"
     CDS             complement(4349..>4563)
                     /locus_tag="VFT30_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092256.1"
                     /translation="PINVTPTFLGKGTTLLQLSYVLLIVLLTWRGLDRSILDPLLMLM
                     VGFTLASGLHYLYRGYRDTNGAPPLA"
BASE COUNT         1085 a         1310 c         1067 g         1101 t
ORIGIN      
        1 gacccgttcg atcagatttt ccaactcccg gacattgccg cgccattcat gcccgagcaa
       61 gatgtgcatc gcctctgggt tgatcacggg tttggcctta ccactctcct tggaaaaccg
      121 ttccagaaag tgattgacca gtaaaggaat atcgctggta cgcatgcgca agggtggaag
      181 ccgtatggga atcacatcca aacgatagta aaggtcttct cgaaacgaac cttccgcaac
      241 cgcttgctcg agatccttgt tggtcgcagc caccacacgt acgtcgactt tgacgtcttg
      301 atttccgccc accctccgaa actcccgttc ttggatgaca cgcaggagct tcacttgaat
      361 cgtcggggtc gtatcgccga tctcgtcgag aaaaatggtt cctccgttag caatctcgaa
      421 caatcccgct ttgttcgaga tcgctccagt gaacgatccc ttcatatggc cgaacaactc
      481 actctccaac aacgtttccg gcactgcact gcaattcacg gccacaaagg ggaggacgct
      541 tctggcgctg ttgtaatgaa tcgcacgcgc gaccagctct tttcctgttc cgctttcgcc
      601 gcagatgagg acattgcttt tcgagtcagc gactttccgt acgacgtcga acaccttctg
      661 catcgcctcg ctttggccga ccagctgcgc gaatgacgac tggcttgcca tttcccgctt
      721 gagaagtatg ttctcggtcg tgaggcgccg cttttccaat gcatttctga taatcaactg
      781 gacttcatca acctggaacg gcttcgtcaa atagtcgtac gcgccctgtt tcatggcctc
      841 aacggcggaa tccgcagtag caaaggccgt gatgatcagc acgaccgttt ccggcgaggc
      901 ggactttacg gccttaagga cctccattcc atcgagcttc ggcatccgca aatcggtgat
      961 gactagatca aagatttcct tgttcagaaa ctcgatggct tcttcgccgt ccatagcact
     1021 ggtcacggca tatccggccc gtttaagcat gatgctcaat acgtcgcgca aactttgttc
     1081 gtcatcaacg actaggatct tttccacggt tctctacctt catgccagag tcgcactcct
     1141 gaatctgccg cgcgaggcaa gcagactcca aatcgtgtcc cttgtccctc ctggctttcc
     1201 actttgatcc accctccatg aagatcaacg atgcgatgga ctgcggccaa gcccaatcca
     1261 gaccctcgct ttttggtggt aaagaacggg agaaaaattt tgtcgagatt tttcttcggg
     1321 atgccttcgc cagcatcctg gaatgagacc tctactacct cggcctttcg cccaacgaca
     1381 tcgacctttc tgcatccggt tgcaatcgtg agttgcccac ccttgggcat ggcatcgaag
     1441 gcattcaccg ctagattcca gaacacttgt ttcatttgat cctgatccac ttgcgcaggc
     1501 aaagccccgg tgcacggagc agccttaatg gtgatgttcg ttctgcttcg tgcttcatgt
     1561 tgtaccagat caagagtctc agcaaggact ttgtttaaat cgtactctgc caaattcagt
     1621 gcaggcggcc tggcatactg aagaaactcg gtgataatag cgtccaatct ggtggcttct
     1681 cgaactgcga tctcgatcag gcgttggctg gtctcatcgg cctcaagatc ttttcgaagc
     1741 atttgcatcg ctccagccaa tgcacctaat ggatttctga tttcgtgcgc catccctgcc
     1801 gacatctccc ccagactggc catccattcc ttccgacgca tttcttcttc gaggtcacgg
     1861 atctgagtga gatctttgaa cacccccacc agccctgttc tttctcctcg ctcgtgcaaa
     1921 ggcgcgagag tcatgccgag aatcaaccga ttgccaccgg attgtttaca ttccacttcg
     1981 aatcgcacat tggcagacac atcaagcctg tgaccgtcat tttgctgatc gggatgccag
     2041 ttaaacactt cccgccaggg acgcccctgt acttgcccaa agctatagcc cgtggcttcc
     2101 tgcgccgcag gattgaaaga agtaatttgg cccttctcat cggtcgtaaa gacgccactg
     2161 ccgatgctgt gaacgatatt ctcatggaat gcctgaaggc ggctcagccc ctgttctttt
     2221 tcgcggagcg attgatctgc ttggcaaagc tgatccgcga gggcgccgct taaaaaaccg
     2281 acgaccagga aggccagacc gtagacaccg aacgcatgga gcgtctcagc ggcactgagg
     2341 tcagcatgtg gcaaccatcc ccatacctca gcaaggccgt agagctgtac attggtcaca
     2401 atcccaaaca ggatgatgca gaggctggcc gtcaaaaggc ccacacgacg gcgcggaact
     2461 aaactggcga ccgttacact gatcacatag agcacagcaa acggactttc aattccaccc
     2521 gttcttgcga ttaataccgt ctcgagcaag aaatcgacgg caatctgagc ccatgcgaat
     2581 tgcacgagaa cttgaggagt ggtgagccgc tggaacagga atgaatagag gatcgtgacc
     2641 gcataggtga agacgattag ggcatagaat gtttcaaccc gttcgccctt cgtaacttcg
     2701 aacgcgagag acaatcccaa gagaagtgta acgagaacga ctcgccaacc cattaaccag
     2761 taaattctgg ctttgatatc tcccacgaaa cgaaactccg cctacaagat aggcttcatc
     2821 gtcatgacgt cccacctgac tttgcggaga tgccggctgg caccactatg cttgggaagt
     2881 acgtcgctcg ttatcgtaac gatgaaagag gctcgccgag aggtcagctg cttcatacct
     2941 ttcgcttgaa gggacgaagc cgttcgcaag gtcgaatcgt cgatttgcta tccgatggca
     3001 gatgccatcg taaagatcgg cagatacatc gcgaccacga taaagccgac ggtgacaccc
     3061 aagaacacca tcatcatcgg ctccaacagg gcggtgagat tcgtcacagc ctgatccacc
     3121 tcgtcttcat agaaatcggc gatttttccg agcatgctat ctaacgcgcc cgttgattct
     3181 cccaccgata tcatgtgtgt caccatcttg ggaaatgtcc cgcttttggc gagcggttca
     3241 gcgatcgtct tccctccact gatactgact ttggcatcaa ggagcgcccc ctccaccacc
     3301 ttgttgccag aggtcttcgc acaaatcgtc aatgcttcca gcagcgggac cccgctcgct
     3361 agaagagtcc ccaacgtccg cgtgaatttc gctacagacg ctttcctaat gagatctcca
     3421 aacacgggca gtttcagcaa gagcttatca agcgctaact tgccttgtgg agtagcatag
     3481 tattttttga ccgccaccac gcttgcaaca attcctccca gaatgatgta ccaatttcct
     3541 tgggcgaaat tactcatgtc gataacaagc tgcgtcggtc ctggaagcgc catctttccg
     3601 ttggacattt ctttgaacat tttttcaaat accggaataa cccaaatcat caggactgta
     3661 atcacgatac cggcgattcc aacaatagcg gcaggataga ccaatgcact cttgatttgt
     3721 cccttcagct tcatcgcctt ctcgatatgc ttagaaagac gccccaagat ggtatccagc
     3781 aaccctccga cttcaccggc atggaccata ttgacgtaca ggtcatcgaa gattttggga
     3841 tgtttgcgaa gggcatctga aaacgtcgaa cctccctcga cctggttttt gatctcaccg
     3901 acagattttc tgagggccgc attctccgat tgcgtcgata agatttccag gcattgaatc
     3961 aaaggcaagc cagcattgat catggtccca aattgcctag taaaaacgac caaatccttt
     4021 tcgctcactc cgcttccgag tttaaaactg aatccccctt tggccgcctt ctcctcgaga
     4081 ctcgtcacca ccacgctttg tttgcgtagt tgctccacag cttcgtcgcg agacttcgca
     4141 atgagttcgc cttttttcac ccccccagat ttcgtccgcc caatatatgc aaacgtggcc
     4201 atagattctc gtcctgttgt ggctccctca gctcctgtac ggaaggctaa gactacgaag
     4261 cgagtctact ggtgggtcgg aaacctgtca aaaacaattg aattatttac cgagctgctg
     4321 aaagaggtcg gaggcacaac ggaagtcgct aggcaagcgg aggagctcca ttcgtgtctc
     4381 gatatcctct ataaaggtaa tgtaaccctg aggccagggt aaacccaacc atcagcatga
     4441 ggagtggatc gaggatagag cggtcgagcc ctcgccatgt caagagaaca atcaaaagga
     4501 catagctcaa ctgaaggagg gtcgttcctt ttcccaagaa cgtcggagtc acattaatcg
     4561 gtg
//
LOCUS       DASTWE010000595         1795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_403942, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000595 DASTWE010000000
VERSION     DASTWE010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403942"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFT30_06205"
     CDS             <1..552
                     /locus_tag="VFT30_06205"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEU5092257.1"
                     /translation="TGRLATVPRSYWGMLVAHCGIAVFIVGVTMVKGFETEKDVRMNV
                     GETASIGDYTFRFDGAQDVVGPNYTAARGIFRVSQDGRETTVLYPEKRRYAVQNQVMT
                     EAAIDPGLLRDLYVSLGEPLDDGAWSVRLYHKPFVDWIWGGCFIMALGGALAVSDRRY
                     RLAWRRQEPVVPASARTARRKVA"
     gene            549..1076
                     /locus_tag="VFT30_06210"
     CDS             549..1076
                     /locus_tag="VFT30_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEU5092258.1"
                     /translation="MNRFLLPLSIFIVVVVFLGVGLRLNPREIPSPLVGKAAPDFSQP
                     QLYDPAKVFSPADLKGKVWLLNFWASWCSGCRTEHPVLMELAKSGEVPIYGMDYKDQH
                     DEAMTWLKRWGNPYPVVGVDDAGRVGINYGVYGVPETYVIDKQGMIRYKQIGPLDPDT
                     VAQKILPLVKQLESQ"
     gene            1073..1534
                     /locus_tag="VFT30_06215"
     CDS             1073..1534
                     /locus_tag="VFT30_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEU5092259.1"
                     /translation="MKWLAFIVLLLSGPAWAGEARPLADDPAAEARLKHLAVELRCLV
                     CQNQTLADSNAPLAEDLRREVREMIAKDMSDKEIIDFLVARYGDFVLYRPPLKATTTL
                     LWVGPFVLLTVGAVVLFITLRRRAHKVIDVPVTDEEHRRVEQLLAEGGKRS"
     gene            1531..>1795
                     /gene="ccmI"
                     /locus_tag="VFT30_06220"
     CDS             1531..>1795
                     /gene="ccmI"
                     /locus_tag="VFT30_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEU5092260.1"
                     /translation="MTATFWLIVSAMTLCVLGLLLRPLLRRPSTVTSEQEKTLPVYRQ
                     QFSELEQDRADGLLTDEQYRTARSELERRVLEEAGSVETPTAAT"
BASE COUNT          375 a          454 c          556 g          410 t
ORIGIN      
        1 acaggtcgtt tagccaccgt gccacgatct tactggggca tgctcgtggc acactgcggc
       61 atcgcagtgt ttattgtcgg agtgacaatg gtgaaagggt tcgaaactga gaaagatgtg
      121 cggatgaacg tcggagagac ggcgtcgatc ggggactata cgtttcgctt cgatggcgct
      181 caagatgtgg ttgggcccaa ctatacggct gcgcgcggca tctttcgtgt gagccaggac
      241 ggtcgtgaaa cgaccgtcct ctacccggag aagcgacgct atgccgttca gaatcaagtc
      301 atgaccgaag ccgccattga tccggggtta ttgcgggatc tctatgtgtc cttgggagaa
      361 ccgctcgatg acggagcgtg gagtgtacgg ctctatcaca agccgtttgt ggattggatt
      421 tggggcgggt gttttatcat ggcgttgggc ggcgcactgg ctgtcagcga tcgacgatat
      481 cgactggcat ggcgtcggca agagcctgtt gtccctgcct cggcacgaac ggctaggcga
      541 aaagtggcat gaatcggttt cttctgccgc tgtcgatttt tatcgtagtt gtcgttttcc
      601 tgggggtagg gcttaggctc aacccgcggg aaatcccgtc tcctttggtc ggaaaggccg
      661 cccctgattt ttcccaaccg cagctctacg atccggcaaa agtgttctcg ccggctgacc
      721 tcaaggggaa agtctggttg ctcaattttt gggcctcctg gtgcagcggg tgtcggactg
      781 aacatccggt gttgatggag ttggccaaat ccggtgaggt gccgatctat ggaatggatt
      841 acaaggatca gcatgatgaa gccatgactt ggttgaagcg gtggggcaat ccctaccccg
      901 tggtcggagt ggatgatgcc ggccgagtcg gcattaatta tggggtctat ggagttcctg
      961 aaacttacgt catcgacaag cagggtatga tccgttataa acaaatcggg ccgttggatc
     1021 ccgacaccgt cgctcagaag atccttcccc ttgtgaagca gctcgaatcg caatgaaatg
     1081 gttggcgttc atagtgctac tcctgagtgg gccggcgtgg gctggggaag ccaggccatt
     1141 ggccgatgat cctgccgccg aggcacgact caagcacctc gccgtcgagc tacgatgtct
     1201 ggtctgtcag aatcaaacat tagccgattc caacgctccc ctggcagagg acttgcgtcg
     1261 agaagttcgg gaaatgatcg cgaaggacat gagcgacaaa gaaatcattg attttctcgt
     1321 cgcacgctat ggagacttcg tactgtatcg accacccctc aaagccacga ccacactgtt
     1381 gtgggtaggc ccgtttgtct tattaaccgt cggagccgtg gtgctcttca tcacgttgcg
     1441 gcgtcgggcg cacaaagtca tcgatgtgcc ggtgacggac gaagagcatc gacgagtcga
     1501 acaattattg gcagaaggag gcaagcgctc atgaccgcta cattttggtt gatcgtttcg
     1561 gccatgaccc tgtgtgtcct cggtctgctg ctgagacctc tgttgaggcg cccgtccacc
     1621 gtcacgagcg aacaggagaa aacgctgccg gtctatcgcc agcagttctc tgagctggag
     1681 caagatcgcg ccgatggatt gctgaccgac gaacagtatc gaacggcgcg cagcgagctg
     1741 gagcgtcgtg tgttagaaga ggcgggtagt gtcgaaacgc caaccgcagc gacgg
//
LOCUS       DASTWE010000596         1399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_662589, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000596 DASTWE010000000
VERSION     DASTWE010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662589"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFT30_06225"
     CDS             complement(<1..615)
                     /locus_tag="VFT30_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5092261.1"
                     /translation="MGQDLQSLIRNFSIIAHIDHGKSTLADRFLEATGAVTAREAKEQ
                     ILDAMDLERERGITIKAHAVAIRYRAKDGKSYLLHLIDTPGHVDFTYEVSRSLAACEG
                     SLLLVDATQGVQAQTIANVNLAMANHHSIIPVINKIDLASADVDGTKQQISEVLTLDA
                     SDAMLVSAKEGLGVPDVLEAIVTRIPPPSGDPDRPLKALIFDSWY"
     gene            753..>1399
                     /locus_tag="VFT30_06230"
     CDS             753..>1399
                     /locus_tag="VFT30_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5092262.1"
                     /translation="MTTTSETARLAAWDHAYLWHPFTQMQEWEQDIPVIIKRGDGCYL
                     IDTEGRKYLDGNSSIWVNLHGHRHPRLDRALRAQVRDIAHSTLLGLSNPPAIRLAREL
                     IRIAPKGLRRVFYSDNGSTAVEVALKMAVQYWQQRDPAAGPKNTFLHLKLAYHGDTVG
                     AVSVGNIELFHARFKPLLFPTLQVDPPYCYRCPLGLTYPSCRMACLDPLEAMLKTR"
BASE COUNT          295 a          425 c          368 g          311 t
ORIGIN      
        1 gtaccaggag tcgaaaatca gcgccttgag cgggcgatcg ggatcaccgg acggcggcgg
       61 aatgcgggtg acgatcgcct ccaacacgtc ggggaccccc aatccttctt tggcgctcac
      121 caacatggca tcgcttgcgt ccaaggtcag cacttcggaa atctgctgtt ttgtgccgtc
      181 gacatccgcg ctggcgagat cgattttatt gatgacggga atgatgctgt ggtgattggc
      241 catcgccaaa ttgacgtttg cgatcgtctg cgcctgcaca ccctgcgtcg catcgacgag
      301 cagcagcgag ccttcacagg ccgcaagact acgcgacacc tcgtatgtga aatcgacgtg
      361 gcccggcgta tcgatcaaat gcagcaaata cgacttcccg tctttggctc gatagcgaat
      421 ggccaccgca tgagctttga tcgtgatgcc acgttcgcgc tcgaggtcca tcgcatcgag
      481 gatctgttct ttggcctctc gagcagtgac tgcgcccgtg gcttctagga accggtcagc
      541 gagggtcgat ttaccgtgat cgatatgggc gatgatcgag aaatttcgga tgagactttg
      601 caaatcctgg cccatgtacc caaaatcggt tcattatagt ggccgcctcc taggttgtca
      661 aagaaatcgg ccgccgttat aattctcgcg acgcagcgat ttacgcgcat aaacattccc
      721 gccgatccat cattcccgac gcacacgcct tcatgaccac aacttccgaa actgctcgac
      781 tcgccgcatg ggatcatgcc tatctttggc acccgttcac gcagatgcag gagtgggaac
      841 aggacattcc cgtgatcatc aagcggggtg atggctgcta cctgatagat acggaaggac
      901 gaaaatacct cgacggcaac tcttccattt gggtcaacct tcacggccac cggcatccac
      961 gattggatcg cgccctaagg gcgcaggttc gggatatcgc tcactcgacc ctgctcggtc
     1021 tctccaatcc accggccata cgacttgccc gcgaactgat tcgaatcgcg ccaaaggggc
     1081 tccgacgcgt gttctattcc gataacgggt cgacggccgt cgaagtcgct ctgaaaatgg
     1141 cggtgcagta ctggcaacag cgagatccgg ctgccggtcc gaagaatact tttctccatc
     1201 tcaagctggc ctatcacgga gacaccgttg gggccgtcag cgtcgggaac atcgagctct
     1261 tccatgctcg attcaaacca ctcttatttc caacgctgca ggtcgatcct ccctattgct
     1321 accgttgtcc cctcggcctg acctacccct cctgtcgcat ggcctgcctt gatcctctcg
     1381 aagccatgtt gaaaacgcg
//
LOCUS       DASTWE010000597         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_727040, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000597 DASTWE010000000
VERSION     DASTWE010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727040"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /gene="pyk"
                     /locus_tag="VFT30_06235"
     CDS             complement(<1..1127)
                     /gene="pyk"
                     /locus_tag="VFT30_06235"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5092263.1"
                     /translation="MSMRKAKIVCTIGPASSSPAILNRLIENGMDAARLNFSHGTHES
                     HATAINAIRKAAAQRGLGVAIIQDLQGPRIRVGLLPKEGIEVNTGQVVRLRTLAQSGE
                     LYDAQASVPATVPEIPVTYASLTRDLHVGSRVLINDGLIELQTVRIADDVIECTVIIG
                     GTITSHKGINLPGTAVSAPTLTEKDREDIRFGAEHGVDYVALSFVRGPQDIDAARVVL
                     AEHGINTPIIAKIERAEAVAALDEILERAEGVMIARGDLGVEMGPEAVPLLQKRITVE
                     ANRRRRLVITATQMLESMTQAIRPTRAEASDVANAVFDGSDALMLSAETAIGAHPIEA
                     LQVMDRIIRAAEEGAEPGVILKRQADLEHLSISESTCAAAS"
BASE COUNT          280 a          372 c          362 g          303 t
ORIGIN      
        1 aaagacgcag cggcacaggt ggattccgag atcgaaagat gctccaggtc ggcttgacgt
       61 ttgaggatga ctcctggttc ggcaccttct tctgccgcgc gtataatccg atccatgact
      121 tgaagcgctt cgatcggatg agctccaata gctgtttcag ccgagagcat cagggcgtca
      181 ctgccgtcga agacggcgtt ggcaacgtcc gacgcctccg ctctcgtcgg acgtatggcc
      241 tgcgtcatcg attccagcat ctgagtggcg gtgatgacca gacgacggcg acgattggcc
      301 tctacggtga tccgcttctg cagaagcggc acggcttcgg gccccatttc gacacccaga
      361 tctcctcggg cgatcatcac accctccgcc cgctccaaga tctcatccaa cgccgcgacg
      421 gcttcggccc gttcgatctt cgcgatgatc ggcgtgttga ttccatgctc cgccaacacg
      481 actcgtgccg cgtcgatatc ttgggggccc cgtacaaaag agagtgccac atagtccacg
      541 ccgtgttcgg ctccgaatcg aatgtcctcc cgatccttct cagtcagggt aggggcgctc
      601 acggcagtac cgggaagatt gatgcctttg tgtgaggtga tcgtgccgcc gattatgacg
      661 gtacattcga tcacatcgtc tgcaatgcga acggtttgta gctcgatgag tccatcattg
      721 atgagcactc ttgatcctac atggagatca cgggtgagtg aagcataggt gactgggatc
      781 tcagggactg tggcgggaac ggaggcttgg gcatcataca gttcacccga ctgagccaag
      841 gtccgtaacc gcaccacctg gccggtgttg acttcaatcc cttccttcgg caacaagccc
      901 acacgaattc tggggccttg cagatcttga atgatcgcca cccctaatcc ccgctgtgcg
      961 gctgcttttc ggatggcgtt gatggctgta gcatgagatt catgggtacc gtgagagaaa
     1021 ttcaatcgag cggcatccat gccgttctca atcaatcggt ttaagatggc cggcgaacta
     1081 ctcgccggac cgatggtaca gacgattttg gcctttcgca tgctcatgtg gaagcgcctc
     1141 caagttttta cccgcaagag agggagagga tatcacagat ccattcgtat acctcatggc
     1201 gacggattgg tgtaaaagca ccacgaatag gttttatctt gaatcagtgc ccacgtgtca
     1261 atgacatagc gaccaaatga ttaccctctc cggttactac gattcggccg ttaggaa
//
LOCUS       DASTWE010000598         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_242824, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000598 DASTWE010000000
VERSION     DASTWE010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242824"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /gene="ligD"
                     /locus_tag="VFT30_06240"
     CDS             complement(<1..1630)
                     /gene="ligD"
                     /locus_tag="VFT30_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5092264.1"
                     /translation="MQKHAASRLHYDFRLELDGTLKSWAVPKGPSLNPSDKRLAVHVE
                     DHPMEYADFEGIIPPRQYGAGTVMVWDRGRWIPDGDPKAAYEKGHLKFTLAGQKLRGR
                     WALVRMGGARNRDEKNWLLIKERDKAARTTTTIDSTRKLSKSVKSGMSMEEIAHVRPD
                     TWLSHRELTRRKQTKISESEKAGSQRGTVACPEWIKPQLATLVDEVPEGDSWIHELKY
                     DGYRMLCRIDRGAVQLFSRNGREWTKKLSEQTQAAGRLAVNNAWLDGELVALNRDGTM
                     SFQALQNAFDRRKDDQLIYYVFDLLFLNGRDLRSRPLYERKRQLASLLDGLPEGGPLR
                     YSDHVLGQGRTVFQTACRRGLEGLIAKQVDSRYVAGRNKHWVKVKCHRRQEFVIGGFT
                     DPSGSRRGFGALLLGVYEGKGNLVYVGRVGTGFSEERLSQLYRALRSLQQRQPAFIKP
                     PTGWDAKGVHWVKPELVAEVRFAEWTQEGLLRQASFVGIRNDKPPGVIVRESAAHVPA
                     SPLMADRRDEEAVDTPNRSSSRAHGVSSPNPTQKH"
BASE COUNT          358 a          490 c          394 g          394 t
ORIGIN      
        1 catgcttctg agttgggttg ggagaactga cgccatgggc acgggaagaa gaccggttcg
       61 gagtatcaac cgcttcttcg tctcgtcggt ccgccataag cggacttgcc ggcacatgtg
      121 cggctgattc acgcacgatg acaccaggtg gcttgtcgtt acgtatgccc acgaatgaag
      181 cttgacgaag gagcccttcc tgcgtccatt cagcgaatct cacctcggct accaattccg
      241 gtttaaccca gtgcacaccc ttggcgtccc agcccgttgg aggcttgatg aatgccggtt
      301 gccgttgttg aagcgatcgc aaggcacgat acagttgaga gagacgttct tcggaaaatc
      361 cggttccgac tcgtcctacg tagaccagat tcccctttcc ctcgtacaca ccgagcagta
      421 atgcaccaaa cccacgccgc gaaccggacg gatcggtaaa gccgccaatc acaaattcct
      481 gacggcggtg gcacttcact tttacccagt gtttattccg cccggcgaca taccgactgt
      541 ccacctgttt ggcaatcaga ccttctaatc cacgccgaca ggctgtctga aaaactgtac
      601 gaccctgccc aagaacatgg tcgctgtatc gcaacggacc gccttctgga aggccgtcca
      661 agagagaggc taattgacgc ttgcgttcgt ataacggacg cgatctgagg tctctgccgt
      721 tgaggaagag aagatcgaag acataatata taagctggtc atcctttcga cgatcgaagg
      781 cattttgcaa cgcctgaaag ctcattgttc catcacggtt caacgcgacg agttcaccat
      841 ccaaccaggc attgttcaca gccaatcggc cggccgcctg cgtctgctct gatagtttct
      901 tggtccattc tctcccattc ctcgagaaca gttgaactgc gccccgatcg attcgacaca
      961 acatgcgata tccgtcatac ttcagttcgt gaatccaact gtctccttcg ggaacttcat
     1021 cgaccaacgt ggccaactga ggttttatcc attcaggaca ggcaaccgtt cctcgctggc
     1081 tacccgcctt ttcagattct gagatcttcg tctgttttct gcgagtgagc tctcgatgtg
     1141 aaagccatgt atcaggtctg acatgagcaa tctcctccat actcattccg cttttcacgc
     1201 tttttgacaa cttgcgcgtt gaatctatgg tcgttgtcgt acgggcggcc ttgtcgcgtt
     1261 ccttgatgag cagccaattt ttttcgtccc gattgcgcgc tccgcccatc cgaaccaaag
     1321 cccagcgtcc ccgcagcttc tgccccgcca gcgtaaattt gagatgtccc ttttcataag
     1381 ccgccttggg atctccatcg ggaatccacc gacctcgatc ccagaccatg acggtgcccg
     1441 cgccatactg ccgaggggga atgatgcctt cgaaatcggc atactccatc ggatgatcct
     1501 cgacatgcac agccaaccgc ttatcagaag gattaaggct cggccccttg ggaaccgccc
     1561 aacttttcag agttccatcg agctccagcc gaaagtcata gtgtagtcga cttgcagcgt
     1621 gcttttggat gacgaa
//
LOCUS       DASTWE010000599         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_597754, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000599 DASTWE010000000
VERSION     DASTWE010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597754"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFT30_06245"
     CDS             <1..1067
                     /locus_tag="VFT30_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5092265.1"
                     /translation="HHWARLLTYVSGLVNEELLLQLEYLAAENRILRAQLPNRLRLTN
                     DQRSTLAEIGKRLGRKGLEKVATAAKPETILGWFRKLVAQKFDGSKYRSYPGRPATSS
                     EVVNLIVRLARDNSSWGYDRIAGALSNLGHKVSDQTVGNILRRHGIAPAPNRSRTTSW
                     KEFIRTHMAVLSGMDFFTVEVLTWRGLVTYYVLFVIQLETRRVILAGMARHPTEEWMQ
                     QVARNLTDTESGTLTHQKYILHDRDAKFCSLFTATLSEGGLQLLKLPARSPNLNAFAE
                     RWVRSVKQECLSKLILFSEASLKRVLAEYLTHYHTERNHQGKENVLLFPAPAVACPQD
                     SPVICKQRLGGLLKYYARAA"
BASE COUNT          279 a          347 c          332 g          285 t
ORIGIN      
        1 gtcatcactg ggctcggctt ctcacgtacg tcagcgggct tgtcaatgag gagctgctgc
       61 tgcagctcga atatctcgcc gccgagaatc ggattctcag agcacaattg ccaaaccggc
      121 tgcggttgac caacgaccag cggtcaacac tcgcggaaat cggcaaacgg ctgggccgaa
      181 aaggcctcga gaaggttgct acagcggcta agccggaaac gattctcggt tggttccgaa
      241 agctcgtggc tcagaagttc gatggctcga agtatcgttc gtatccaggc aggccggcta
      301 ccagctccga agtcgtaaat ctgattgttc gcctcgcccg cgacaactcc agttggggat
      361 atgaccgtat cgctggcgct ctgtctaacc taggccacaa agtctctgat cagacggtcg
      421 gcaacattct ccgacggcat gggattgcgc ctgctccgaa ccgtagtcgg acaacgagct
      481 ggaaggaatt catcagaacg catatggcgg ttctgtccgg gatggacttc tttaccgtcg
      541 aggtgttgac ttggcgaggt ctggtcacct attacgtttt gttcgtgatt caactggaga
      601 cgagacgagt catccttgcc ggcatggccc gacacccgac cgaagaatgg atgcaacaag
      661 ttgcccggaa tctgacggat accgaatccg gcacgctcac tcatcagaaa tacattctgc
      721 atgaccgcga tgcaaagttc tgttcattgt ttaccgcgac cttgtcggaa ggcggtttgc
      781 aactgctcaa acttcctgcc cgctcgccca atctgaatgc ctttgcggaa cgatgggtcc
      841 gatctgtaaa gcaggagtgt ctatccaagc tgatcctatt tagtgaggca tccctgaaaa
      901 gggttctagc cgaatatctc acccactatc acacggagcg aaaccatcaa ggtaaggaaa
      961 atgttctcct gttccctgcc ccggctgtcg catgtcctca ggattcaccg gttatttgca
     1021 agcagcgtct cggcggtttg ctgaagtact acgcacgcgc tgcatgactt ctttgaccat
     1081 acggggtccc ttgggttctt atacgattgg caacgaactc agactgctgc gtcaggctag
     1141 tttccggcaa cgggactcat cgggcacaac ccatcctgga tcggagccgc cttggcattt
     1201 acgtatgcgc caacaacaca tgttgtggcg cattgcaaga acc
//
LOCUS       DASTWE010000600         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1470670, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000600 DASTWE010000000
VERSION     DASTWE010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470670"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFT30_06250"
     CDS             complement(<1..700)
                     /locus_tag="VFT30_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEU5092266.1"
                     /translation="MNIPGFPRQQGLYDPQHEKDSCGIGFVVNIKGKKSHDIVRKGLQ
                     VLENLTHRGAQGCDPCTGDGAGILLQVPHTFLKRVTGDAGVSLPEIGEYGVGQLFLPP
                     NAESRGLCEKLLAEILVEEGLRLLGWRDVPVKSDQIGAQARTTEPFMRQVFIARDALN
                     EAQFERKLYVIRKRVEKAVADSAIQGREHFYVSSLSAHTIVYKGLLLPHQMAAYYQDL
                     TDERMVSALALVHSR"
     gene            924..1928
                     /locus_tag="VFT30_06255"
     CDS             924..1928
                     /locus_tag="VFT30_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5092267.1"
                     /translation="MAQGPVITTLHSMSDVWDAYRGELDGVEDRVRKNLDSSVTLVNT
                     VAAHILSGGGKRIRPLLLLLSAHLCGYPGTEHHQLGSIVEFIHTATLLHDDVVDDADL
                     RRGRRTARKVWGNQISILVGDYLYTKAMCKVVEFQSQGINEVLAEACKKMAEGEVLQL
                     YYNGNPSMPEIDYIKIVEHKTAGLIAAACRMGAILAEASETQQKALFRFGQYLGIAFQ
                     VADDTLDYIANGESLGKTIGQDLRQGKATLPLLHLLQHCSEQDRQMIKDRMETRTLST
                     ADLERILLLMADFGSITYAMDRASAYIDAAKHELERFDDSTARRALSVAADYMITRDR
                     "
     gene            2052..>2266
                     /locus_tag="VFT30_06260"
     CDS             2052..>2266
                     /locus_tag="VFT30_06260"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEU5092268.1"
                     /translation="MSQIFPFQGMLYDQTLVGSIKDVVAPPYDIIDAEGQKRLHDRHP
                     HNIIRMELGIDQTGDSPVHNRYTRAAAT"
BASE COUNT          510 a          652 c          605 g          499 t
ORIGIN      
        1 atcgtgagtg caccaaggct agcgcgctca ccatccgttc atcggtgagg tcctggtagt
       61 aagccgccat ttgatgcggc aatagcagcc ccttataaac gatggtgtgc gccgataggc
      121 tggatacgta aaagtgttct cgcccttgaa tggccgagtc tgccacggcc ttttcaaccc
      181 gcttgcggat cacatacagc tttcgctcga actgagcctc gttcaaggca tcgcgtgcga
      241 tgaagacctg ccgcatgaac ggctcggtcg ttcgcgcttg ggcaccgatc tggtcacttt
      301 tgaccggcac atcgcgccaa ccgagcagac gcaggccttc ttccacaaga atctcggcga
      361 gcaacttctc gcacagccct cgggattctg cattgggcgg cagaaataac tgccctactc
      421 cgtattcgcc gatttccggc aatgacacac cggcatcccc tgtgacccgc ttcaggaatg
      481 tgtgcgggac ctgcaaaaga attccggctc cgtctcccgt gcagggatca caaccctgcg
      541 ctcctcgatg ggtaagattc tccaacactt gaagtccttt acggacaatg tcatgggatt
      601 tcttcccttt gatattgacg acaaagccga tcccacaaga gtccttttcg tgctgaggat
      661 cgtagagccc ttgttggcgg ggaaacccag gaatattcat gtacccatct cgttcaaagg
      721 atgagagttt tccctatggt atggagatga agccagcgag aaaccttggg tggatttgcc
      781 ccctttacgt tcagggtgaa ccgcacccga ttgctaggat gggaccttac tggatgggtc
      841 attcttctgt caaggttacg ataggcggta tacctctctc atttgcttga ctttatttcc
      901 tcggctgcca taccatccgc cgcatggcac aaggtccagt gatcacaacc ctgcacagca
      961 tgtcggatgt gtgggacgcc taccgtggtg aactggacgg agtggaggat cgagttcgga
     1021 agaacctcga ctccagcgtg accttagtca acacggtggc cgcccacatc ttaagcggtg
     1081 gcggcaaacg catcagaccc ctgctcctcc tcttgtcggc tcatctctgc ggctatccgg
     1141 gaaccgagca ccatcagctg ggcagcatcg tggaattcat ccataccgcc accctgttac
     1201 acgacgatgt cgttgatgac gccgatctcc gaagaggccg gagaaccgcg cgcaaggttt
     1261 ggggaaatca gattagcatt ctggtcggcg actacctcta tacaaaagct atgtgcaaag
     1321 tcgttgagtt ccagagtcaa ggcatcaatg aagtcctcgc cgaggcctgc aagaaaatgg
     1381 cggaaggtga ggtccttcag ctgtactata acgggaatcc ctcgatgccg gagatcgact
     1441 atatcaagat cgtcgagcac aagaccgccg gcttgattgc cgcggcctgc cgcatggggg
     1501 ccattctggc cgaggcttct gaaacgcaac agaaggcgct gttccgcttc ggtcagtacc
     1561 ttggtatcgc ctttcaagtc gccgacgata ccttagatta catcgcaaac ggggagtcgc
     1621 tcgggaaaac aatcggacag gacctccggc agggaaaagc gacactgccc ctgctgcatc
     1681 tgttgcagca ttgctcagag caggaccgcc agatgatcaa ggaccgaatg gaaactcgga
     1741 ctctcagcac cgccgacctt gaacgaatct tattgttaat ggccgatttc ggctcgatta
     1801 cctatgcgat ggatcgcgcg agcgcctata ttgacgcggc caaacatgag ctcgaacgct
     1861 tcgacgacag taccgcacgg cgcgcgctgt cggtcgccgc tgattatatg attactcgcg
     1921 accggtagtc ttcccgccag ctggaaggac gctgggccat gggaatggca cggcgtaggg
     1981 cgaaaggatc ccactctctc ctgtaaccat caaaccgtcg tcgctgtcac gacattacca
     2041 agaggttgtt catgtcacag atctttccgt ttcagggtat gctgtacgac cagacgctcg
     2101 tgggctccat caaggatgtg gtcgccccgc catacgatat tattgatgcg gagggacaga
     2161 aacgtcttca cgaccgccat ccccacaata ttatccgaat ggaattaggg atcgatcaga
     2221 ccggtgacag tcccgtccac aaccgatata cccgcgctgc ggctac
//
LOCUS       DASTWE010000601         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_824411, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000601 DASTWE010000000
VERSION     DASTWE010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824411"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFT30_06265"
     CDS             <1..404
                     /locus_tag="VFT30_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5092269.1"
                     /translation="EKKQDVFVVATANDLSSLPPELLRKGRFDEIFFVDLPDDAERES
                     IWTIHLGLRKQDSKKFDLGKIVSASDGFSGSEIEQAVVAALYRALHQKTSLTTDLLIE
                     ELTHTVPLSVTRREDIDALRQTAEGRFVNVR"
     gene            497..1240
                     /locus_tag="VFT30_06270"
     CDS             497..1240
                     /locus_tag="VFT30_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU5092270.1"
                     /translation="MVPSISADKMKPRVSSPLPQPSPTSATVDHLTSFMTTLPNRSAI
                     VESAARLVGTRTITSQGRRIAYDCAGVTRAIFLEHGIDLYRGAFNDPRGNGVRLIHNH
                     VRQHGTLHRGTTVSPGDLVFFDNTWDFNGDGKLNDPLTHVGVVERQEPDGTVVFISRV
                     ANAVERYHMNLDQPHVHKNADGRVLNDYIRRKHPADPDQTGRLTGELFSLYGNLLSPS
                     KHISSERTFTQNSNQRRASVSLSSELPKQ"
     gene            1446..1877
                     /locus_tag="VFT30_06275"
     CDS             1446..1877
                     /locus_tag="VFT30_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092271.1"
                     /translation="MATSSKQTHVREINPLRRTLTDVRHGLLSLHKALIVAEQLTFER
                     IYGRVDSTGQLLQLVMNDPWFTWLHPLSNMVVRIDELLDGHDQLTVDDVAMLLTEVRA
                     LIRPSELGDGYERSYYEALQRAPDVVLAHCEMKKLLTLPSI"
BASE COUNT          525 a          603 c          522 g          464 t
ORIGIN      
        1 aggaaaaaaa gcaagatgtg ttcgtcgtcg ccacggcaaa tgatctgtca tcactacctc
       61 ccgaactcct gcgcaaaggg cggttcgatg aaatcttttt cgtcgatctg ccagacgatg
      121 ccgagcggga aagcatctgg acgattcacc tgggactccg gaagcaggac agcaagaagt
      181 tcgacctcgg caagatcgtc agcgcaagcg acggctttag cgggtcggag atcgagcagg
      241 ccgtggtagc agccctctat cgagcgctgc accaaaaaac ttcgctcacg accgacctgc
      301 tcatcgaaga attgacccac acagtgccgc tctccgtcac gcggcgtgaa gacatcgatg
      361 cgctccgaca gacagccgaa ggtcgatttg tgaacgtgcg gtaacaccaa tgtcattctt
      421 cctgatcatg atcccccttg cgattgcgac tctcttgggt tgcaccaagc ctgcccagaa
      481 gctgaatagg gaaaccatgg ttccatcaat ctcagcggat aagatgaaac ctcgtgtatc
      541 atctccactg cctcagcctt ctcctacatc ggccacagtg gatcacctta cttccttcat
      601 gacgactctt cccaaccgat cagccatcgt cgagtccgca gccaggctgg tcggaacgcg
      661 gactattaca agccagggca gacgtatcgc ctatgactgt gcaggggtta cccgtgccat
      721 cttcctggag cacggcatcg atctgtatcg tggagcgttc aatgatccga ggggaaacgg
      781 cgtccggctc atccacaacc atgtgcggca gcatggcact cttcatcgag gaacaaccgt
      841 cagtccaggc gatctcgtgt ttttcgataa cacgtgggat ttcaacggag acggcaagtt
      901 gaacgatcct ctaacccatg tgggagtcgt cgaacggcag gaaccagacg gcacggtggt
      961 cttcattagc cgcgtcgcca acgcagtcga gcgctaccac atgaatttgg accaacctca
     1021 tgttcataag aatgccgacg gccgcgtgtt gaacgattac atacgacgga aacatccagc
     1081 tgatcccgac cagacgggcc gtttgaccgg tgagctcttt tccctctatg gaaacctgct
     1141 aagcccttca aagcatattt cttcggaacg aacgttcaca cagaattcga accagcgacg
     1201 ggcttctgtc tccttgtcct cagaattacc caaacaatga cgctgacttc cgatgcgcca
     1261 ctgcgcaacc cgatccgatg attgctatag gtgagaatgt ggcctttaac tcaaatatac
     1321 aatgacccaa gttgcgacta cagaacaaac ccatgatatc cgataaggaa catatcgtgg
     1381 cattaatcat attgagaacc gacgctaagt cacaattctt ccattgacct ggtaagatta
     1441 tttctatggc tacctcatcg aaacaaacac atgtccgaga aatcaacccg ctccgtcgaa
     1501 ccctcacgga tgtccggcat gggctcttaa gtttgcataa ggccctgatc gtcgccgaac
     1561 agctgacctt tgagcgaatt tacgggcgtg tggactctac gggccagctc ctccagttgg
     1621 tgatgaacga cccgtggttt acctggctgc atccgctttc caacatggtc gtccgtatcg
     1681 atgaactgct cgatgggcac gaccaactga cggtcgacga cgtcgcgatg ttgttgaccg
     1741 aagtgcgggc gctcattcgc ccctcagaac tcggtgacgg atatgaacgg agttactatg
     1801 aggcactgca acgcgccccc gacgtcgtcc ttgcacactg cgaaatgaag aagctgttga
     1861 cgctgccctc gatataaaga cctccccgac tcttcgagtg atgacgaata aaagccgtcg
     1921 gcgcataaga gccgacggct ttgtcgcctt gtctgttcga caaagcctag ccggaagttt
     1981 gcaccactcg acaaagaagc tgcatgacgt agatagacgt tgtctacaac gtccggtgcg
     2041 atctcttgca tgattcgcca taactaagca acactgaatt agctctacga gcgagatgca
     2101 gatgcttttt atca
//
LOCUS       DASTWE010000602         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_242878, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000602 DASTWE010000000
VERSION     DASTWE010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242878"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VFT30_06280"
     CDS             complement(<1..1078)
                     /locus_tag="VFT30_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092272.1"
                     /translation="MAIEAADRRKAEQALRTSEGRLTMTVQNTSIGIWDWDISSNDVY
                     LSPEWKRQLGYEDHELDNTFQEWEGRLHPEDHDRALGAIKAYLGRRESQFELEHRLRH
                     KNGSYRWILSRGTLITNEGELSTRMIGIHIDVTDRKTAEEMLRQAKEAAEAANRAKSQ
                     FLANMSHEIRTPMNGVLGMAELLLRCSLSDREYHLAESIHRSGTVLLAIINDILDFSK
                     IEAGKLQLETIPFEVQRTIQEAVDLSGPAAQKKQLKLSWQIDQNIPVYLLGDPTRLKQ
                     IVVNLVENAIKFTEQGLIEVSVMLESEQEEQYGLSVTVRDTGIGISSEVQAHIFDAFC
                     QADGSTTRKYGGTGLGLAIVKQLVT"
BASE COUNT          228 a          313 c          267 g          281 t
ORIGIN      
        1 atgtgacaag ctgcttgacg attgcaagac ccaacccggt cccaccatat ttccttgtcg
       61 tcgaaccgtc ggcttggcaa aaggcatcaa agatatgggc ttggacctcg gaagagatgc
      121 cgatgcctgt gtcacgtact gtgaccgaca atccatactg ctcctcttgc tcactctcaa
      181 gcattacaga cacctcgatg agcccctgtt ccgtaaattt gattgcattt tcgaccagat
      241 ttacgacgat ttgtttcagc cgtgtcgggt cgccgagaag atacacagga atattctgat
      301 caatctgcca agatagtttc agctgcttct tctgggcggc cggtcccgac aaatcgacgg
      361 cttcctgaat cgttcgttga acctcaaacg gaatcgtttc gagctgcaac ttaccggctt
      421 caatctttga aaaatccaga atgtcgttga tgatcgcgag cagcaccgtc ccggatcgat
      481 ggatcgattc agccagatgg tattccctgt cgctgaggga gcatctaagc aatagctccg
      541 ccattcccag gactccattc atgggtgtcc gaatttcgtg gctcatgttc gccagaaact
      601 gactcttggc gcggttggcg gcttcggcag cctccttggc ctggcggagc atctcctccg
      661 cagttttgcg gtccgtcaca tcgatatgga tgccgatcat gcgggtcgac agctcgcctt
      721 cattcgtgat caacgtcccg cgggaaagaa tccatcgata cgacccattt ttatgacgga
      781 gacggtgttc aagctcgaat tgagattctc ttcggcccaa ataggccttg atggcgccca
      841 atgcacggtc gtgatcctca ggatgcagcc ggccttccca ttcctgaaag gtattatcga
      901 gctcgtgatc ctcgtatccc agttgcctct tccattcggg cgataggtac acatcattcg
      961 agctgatgtc ccaatcccaa attccgatgc tggtattctg aaccgtcatg gtcaggcgtc
     1021 cttcgctggt tcgcaacgcc tgctcggcct tccgtctgtc ggcggcctct attgccaagg
     1081 ccaccgtat
//
LOCUS       DASTWE010000603         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_953465, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000603 DASTWE010000000
VERSION     DASTWE010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953465"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFT30_06285"
     CDS             complement(<1..273)
                     /locus_tag="VFT30_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HEU5092273.1"
                     /translation="MTDEQAMVEAFHNKFDILVQTTPTDLNEETKHLRVRLIQEEFDE
                     LKEAMATGNLAAVAKEMADLLYVTYGTAVSYGIDMEPVFREVHRSNL"
     gene            complement(284..>1152)
                     /locus_tag="VFT30_06290"
     CDS             complement(284..>1152)
                     /locus_tag="VFT30_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU5092274.1"
                     /translation="QSRQSAYGGLDQHLRLLAESQQRLQQETGNLVKALRAPTVRGQW
                     GELTLKRVAELAGMVDHCDFVEQPSVTVDDGRFRPDMVVQLPGGRQIIVDAKTVLSAY
                     LDAHEAQNEAQQMEALRRHAAQVKCRMDELSLKAYWTQFDRAPEFVVLFLPGEQFLGA
                     ALDQNPRLIEEGFANGIVLATPATLIALLRAVAYGWRQECMTAHAEEAGRLGKELYER
                     MAVLAEHMNDVGQALGKSVSAYNRAVGSLETRILPAARRFKELGVASDRDIPVLGPME
                     VVPRKTLPFDIE"
BASE COUNT          243 a          358 c          287 g          264 t
ORIGIN      
        1 cagattcgac cggtgcactt cccgaaacac cggttccata tcgatcccgt aggacaccgc
       61 ggttccatag gtcacataca ggaggtccgc catttctttg gccactgcgg ccaaattccc
      121 ggtggccatg gcctctttca attcatcgaa ctcttcctga atgagtcgaa cgcggaggtg
      181 tttcgtctct tcattcaaat ccgtgggagt tgtctgtact aaaatatcaa acttgttgtg
      241 aaatgcttcg accatggctt gttcgtcggt catgcctact ccttcactcg atatcaaacg
      301 gcaaggtttt gcgtggaaca acttccatcg gtcccaacac cgggatgtcg cgatccgacg
      361 ctacccccaa ttccttgaag cgccgtgctg ccgggagaat gcgtgtctca agagaaccga
      421 cggcccggtt ataagctgac acgcttttgc cgagagcttg gcccacatcg ttcatatgtt
      481 ccgccagcac agccatgcgc tcatacagtt ccttgcccaa tcgaccggct tcttctgcat
      541 gggcggtcat gcattcttgg cgccagccgt aagcgaccgc ccgaagcaag gcaatgagcg
      601 tcgccggcgt cgccaacacg attccgttcg caaacccctc ttcaataaga cggggattct
      661 ggtccagcgc cgcccccaag aactgctctc ctgggagaaa caacacgaca aactctggtg
      721 cacggtcgaa ctgagtccag tacgctttca gagacagttc gtccatgcgg catttgacct
      781 gagcggcgtg gcgacgcaag gcctccatct gttgggcctc gttctgggct tcatgggcat
      841 ccaggtaggc cgagagcacg gtcttcgcat ctacgataat ttgccgtcct ccaggcaact
      901 ggacgaccat atccggtcga aaacgcccat catcgacggt cacagagggc tgctcgacga
      961 aatcgcaatg gtccaccata cccgcgagtt cagccactcg tttcaacgtc aactcgcccc
     1021 attggccccg tacggtcgga gcccgcaagg ctttcactag atttcctgtc tcctgttgca
     1081 gccgttgttg cgattccgcc aataatcgta gatgctgatc caatccgcca taggccgact
     1141 ggcgagattg ct
//
LOCUS       DASTWE010000604         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_210356, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000604 DASTWE010000000
VERSION     DASTWE010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210356"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFT30_06295"
     CDS             complement(<1..454)
                     /locus_tag="VFT30_06295"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEU5092275.1"
                     /translation="MKPAGQMMKQVLRGGSAVIASLIIGGASLTFAQESVSVRASLVT
                     GGLPLDDPNAAAWSSAAPASFPMSPQVHWPNRIQEVTVKDVSVRALHDGKQVVFLLEY
                     ADPTQDLDDGAALEFMVGDKKAHFAHGQPMLQVEGGPVNIWFWKNKAGK"
     gene            complement(506..1222)
                     /locus_tag="VFT30_06300"
     CDS             complement(506..1222)
                     /locus_tag="VFT30_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5092276.1"
                     /translation="MGALGKPIILMVAMYLFLKFVLPNIPGSAPLPSSLIFLYLLLTA
                     SGIVIYETLSGESKDAFWGPMQRFLTGEKIGGLQAIRYGVLILFPLLVGWQTYGSTAT
                     SDLPPAESRTIHPAPPGEYTGLSNPVPKTPENIMQGKGFYAAFCSPCHGGNFDGKGPA
                     ARGFNPPPANFADPTTIAMLQESYLFWRIKKGGVGLPIEGAPWKSAMPRWEVELPDEW
                     IWKIIMGEYDGAHQSPRTWE"
     gene            complement(1370..1996)
                     /locus_tag="VFT30_06305"
     CDS             complement(1370..1996)
                     /locus_tag="VFT30_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5092277.1"
                     /translation="MSEVAQLQLIALSIVGIGILILLFIKATFVRVTGFVAIVLGLFS
                     LTALAVPQLASLPPAEEKIDIASIKTPTDIAAIGQTVFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSRDFLYESLTDPQAFVYHDYRHGGVPKEYPATMPAINKDPIGLSKNEI
                     LAVIAFLQQMSGEPISISVTDLEIPGQAPSAPVKAAESSLVADASRNQ"
     gene            complement(2054..>3973)
                     /locus_tag="VFT30_06310"
     CDS             complement(2054..>3973)
                     /locus_tag="VFT30_06310"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU5092278.1"
                     /translation="KAELPRTPLPRRNRNLRGDEVRAIQQGVGPIQRALRAVGGHHGW
                     LAGLLLTAGWLILPSIGLAAEGTAAGPTEYRDIPYIGSRNLIWIVAQLHLLLAGFVLG
                     VPIFAWLCEVIAWRGGEQRYDKLAKEFTKLLTSAYATTALFGGILLFLLVAFYPKLMN
                     YLTDIFFPSFLVYCLLFLLETATLYLYWYGWDAMQYGNKKTLHIFLGFLLNFFALFIM
                     IVPNAWATFQSSPVVISEGTAFERAWAATWNPTWWPINVHRLIANVVLGGYICGAYAG
                     VRYLSVKNPEDREHYDWMGYVGNFIGVFGLLALPFAGYWLMREIYQYNQQMGITLMGG
                     FLSWLFIIQAMLIGVLFLGSNYYFWLGITYRIPGSEAKYRRAMLLMLISLLFCLAVWM
                     TPHSLVASIEEAQKMGGAHHPLLGVLGVMSAKMTVSNLMILITFMSFVMYWRAGKQDT
                     AGWAKLGKAVMGAVLVLASLAVIVLGVWGYFVPAIVRINYFSTSQVLIVIFVILTIAP
                     LTALLLKSARTTTEMVWGSMPPRAGYALVLNAVMVILLMTLMGYARSSSRVHWHVYGV
                     MRDSSPYAYSPALGSASLIMAFCTFFFCILVAFIFWVATMGDKAKAASAPGKGELPHG
                     IPAMAGGSPEERQQE"
BASE COUNT          948 a         1156 c         1049 g          820 t
ORIGIN      
        1 ccttgcctgc cttgtttttc cagaaccaaa tattaaccgg cccaccttcg acctgcagca
       61 tcggttgccc atgggcaaaa tgcgccttct tgtccccgac catgaattcg agcgccgccc
      121 cgtcgtccag atcctgagtg ggatcggcat attccagaag aaatacgacc tgcttcccat
      181 catgcaacgc acggacggac acatccttga cggtgacttc ctggatgcgg ttcggccaat
      241 ggacttgcgg cgacatcggg aaggacgccg gggctgcact gctccaggct gcagcattcg
      301 gatcgtcaag ggggagccct ccggtcacaa gcgaagctcg aaccgacacg ctctcctgtg
      361 caaacgtgag agatgcccca ccgatgatca gacttgcgat cactgccgac ccaccgcgca
      421 gaacctgctt catcatttga cccgccggtt tcatactggc ctcgttctcc ctacacactt
      481 cgatccatcc acgttgtcct tgttcctact cccaagttcg gggggactga tgagctccgt
      541 cgtattcgcc catgatgatc ttccaaatcc attcgtccgg taattccact tcccaacgag
      601 gcatggcgga cttccatggc gcaccttcaa tcgggaggcc gaccccgccc ttcttaatgc
      661 gccaaaacaa atagctttct tgcagcatcg caatggttgt cggatcggca aaattggccg
      721 gaggaggatt aaagccgcga gcggccggtc cctttccatc aaagttgcct ccatgacagg
      781 gggagcaaaa tgccgcataa aatcccttcc cctgcatgat attttctggg gttttgggaa
      841 caggattcga caacccggtg tattccccag gcggtgcagg atgaattgtt ctgctttccg
      901 ccggtggaag atcgctcgtg gccgtgctgc cgtaggtttg ccatccgacc agcaaaggaa
      961 acagaatgag caccccgtat cgaatagctt gcagacctcc gatcttctct ccggtcagaa
     1021 atcgctgcat cggtccccaa aaggcatctt tggactctcc gctcaatgtc tcgtagataa
     1081 cgatgccgga cgcagtcagc aggagataga ggaaaatcag gctggaggga agaggcgccg
     1141 agccgggtat attcggcagc acaaatttca gaaacaggta catcgctacc atgagtatga
     1201 ttggcttccc taacgcgccc atacgttcct ccctagcagg atgttgaaaa agttctccag
     1261 ctgcgttctc acatcgcgca gaggctcaac gtacgataaa aagtacgctt cgcctctttg
     1321 ctcgttgcgg tcttgctgga aggccatttt gaacatcctg acaatgtgct cattgattcc
     1381 gtgatgcgtc ggcgaccaac gaagattccg ctgctttgac tggagctgac ggcgcctggc
     1441 ccggtatctc caggtccgtt acgctgatgg agatcggctc accgctcatt tgttgcagaa
     1501 atgcgatgac ggctaaaatt tcattcttag agagcccgat cggatcttta ttgatcgccg
     1561 gcatcgtcgc tggatattcc ttcggcaccc ctccatgtcg ataatcgtgg tacacgaacg
     1621 cctgaggatc cgtcagactc tcatagagga agtcccggct caatttggca ccgatcccct
     1681 tcaaatccgg gcaacgggcg gattcgctgg gaccgatgga atggcacagc gcacactggc
     1741 ctttgctgaa gaataccgtc tgcccgatag cggcgatgtc cgtcggtgtc ttgatactgg
     1801 cgatgtcgat cttttcttca gccgggggca gcgacgccaa ttgcggcacg gcgagagctg
     1861 tgagcgaaaa cagcccgagt acgatggcga cgaaccctgt gactcgaaca aaggtggcct
     1921 tgatgaagag gaggatcaga atcccgatcc cgacgattga cagtgcgatc aattgtagtt
     1981 gtgcgacttc actcatagtt ccccaactcg taaggatatc gttttctgtc tttttgattc
     2041 atgcggctgt cacctattcc tgttgccgtt cctccggcga ccctccggcc atcgccggaa
     2101 tgccgtgcgg caactctccc ttccctggcg ccgaagccgc cttagccttg tcacccatgg
     2161 tagcgaccca gaagataaac gccacgagga tgcagaagaa aaacgtacag aaggccatga
     2221 tcagcgacgc gctgccgagg gccggcgaat aagcataggg cgacgaatcc cgcatcacgc
     2281 cgtacacatg ccaatggacg cgggacgagg atcgtgcata gcccatcaac gtcataagaa
     2341 ggatgaccat gacggcattg agaaccagtg catatcctgc gcgcggaggc atactgcccc
     2401 agaccatctc tgtcgtcgtc ctggcgctct tgagcaacag cgcggtcaac ggcgcgatcg
     2461 tcagaatgac aaagatgacg atcagcacct gggaggtgga gaaataattg atgcggacga
     2521 tcgccggcac gaagtatccc cacactccga ggacgatgac agcgagactg gccaacacca
     2581 acacggcacc catgaccgcc tttccaagtt tggcccaccc ggctgtatct tgcttccctg
     2641 ccctccaata catgacgaag ctcatgaagg taataagaat catgaggttc gacaccgtca
     2701 ttttggcaga catgacgccc aggacgccca gtaacgggtg atgggctcct cccattttct
     2761 gagcctcttc gatgctggcg acgagcgagt gtggtgtcat ccagacagcg agacagaaca
     2821 gcaagctgat cagcatcagc aacatggccc gccggtactt ggcctctgat ccgggaatgc
     2881 gataggtaat ccccagccaa aagtaatagt tcgaaccgag gaacaggact ccaatcaaca
     2941 tggcttggat gatgaagagc caagacagga atcctcccat caacgtaatg cccatctgct
     3001 ggttgtattg gtaaatttcc cgcatgagcc aatagccggc aaacggcaaa gccaacaggc
     3061 cgaacacgcc gatgaaattg ccgacatagc ccatccaatc gtaatgttct cgatcttcgg
     3121 gattcttgac cgacaagtac cggactccgg cataggctcc gcagatgtac cctccgagca
     3181 caacgttggc gatgagccga tggacgttga tcggccacca cgtcggattc cacgttgcag
     3241 cccaggctct ctcaaaagcg gtgccttcgg aaatgacaac tggactggac tggaaggtcg
     3301 cccaagcatt aggcacaatc atgatgaata aggcgaaaaa gttcagcagg aaccccagaa
     3361 atatgtgcaa cgttttctta ttgccatact gcatcgcgtc ccacccgtac caatacagat
     3421 agagcgtggc ggtttcgagc aggaacagaa ggcagtagac caaaaaggac gggaagaaga
     3481 tatccgtcaa ataattcatg agtttggggt agaacgccac caagagaaac aggagaatgc
     3541 cgccgaacag ggcggtggtc gcgtaggccg aggtcaaaag cttggtaaac tccttcgcga
     3601 gcttatcgta acgctgctcg cctcccctcc aagcgatgac ttcgcacaac cacgcaaaga
     3661 tcggcacgcc caagacgaac ccagccagca ggaggtgcag ctgcgcgaca atccagatga
     3721 gattccgact gccgatgtag gggatatcac gatattcggt ggggccggct gctgttcctt
     3781 ccgccgcgag cccgatcgaa gggagaatca accagccggc cgtcaacaag agacccgcga
     3841 gccatccgtg gtgacctccg acggcccgga gagcccgttg aatcggtccg accccttgtt
     3901 gtatggcacg cacctcatca cctcgcaaat tacggtttcg tcttggcaga ggagtccttg
     3961 gcagttccgc ctt
//
LOCUS       DASTWE010000605         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_113409, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000605 DASTWE010000000
VERSION     DASTWE010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113409"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1742
                     /locus_tag="VFT30_06315"
     CDS             19..>1742
                     /locus_tag="VFT30_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092279.1"
                     /translation="MTAYEVRFSDTSLNDNNFDLATPLPGPVPNDPGFFQFVTVRVPW
                     RHPSGFLGIRAVDDVGNKGPLSSLPISVATDVGDPYTIAESAAAPVSTGGTALGLIGD
                     DQFKQINLPFVFNYFGVDFQSVNVSTNGALYFGFPPDEDAFSSERWVNGRRVIAGLWD
                     DLRTDRRPGDDVYMVQDADRVIFRWQAVTFDNPIGPTTTRGENPVSFEIELRFDGTIT
                     LRYGDGNQKTLPVIGIGGGSPEPYISTSHSSESSLKDLTNAATVVFARRKPIQRGVLT
                     VASINPASGVNITVSPNDSTGAGAGTTQFTRTYNSGTTVTLTAPATVNGNKFQHWLRD
                     GQDWSGSLTTSLAMHTNLNATMTAVYYTPPVLTVTSVNPSSGVEISINPNDVNGAGNG
                     TTPFTRTYDLNVQANLVAPATVGTSTFWKWQLDGVDFTPGRSIGLAMSKAYTLTAVYI
                     AATPTPTPTPVPGAGAQPIAFTKPGNGGGADVFLTNIDGTNVVNLTDAAGEDTRPAWS
                     PDGSRLAYTCLRQPDGSIGPPQRICVRNADGTGLVVMSKSLQNEAGPNWSRDGNQIGF
                     TTSPGLQST"
BASE COUNT          400 a          487 c          468 g          387 t
ORIGIN      
        1 ggaaatgcgg gtggtaaggt gacggcttac gaagttcgtt tctccgatac gagcctcaac
       61 gacaacaatt ttgacctcgc gacgcccttg cctggtccag tgcccaacga tcccggtttt
      121 ttccaatttg ttaccgtcag agtgccatgg cgacaccctt caggttttct tggcatccgg
      181 gcggtggatg atgtcgggaa caaaggtccc ctctcctcat taccgatttc agtggctacg
      241 gacgttggcg atccttacac gattgccgaa tcggcagccg ctccggtttc gaccggaggc
      301 acagcgttgg gactgatcgg cgatgaccag ttcaagcaga ttaatttgcc gtttgtcttc
      361 aattacttcg gcgtcgactt ccagtcagta aacgtctcca ctaatggcgc attgtatttt
      421 ggttttcctc ccgacgagga tgcgttcagc tctgaacgat gggtaaacgg acgtcgcgtg
      481 atcgccggct tgtgggacga tctgcgaact gaccgtcgtc ccggtgacga tgtttacatg
      541 gtccaggatg cggatcgcgt aattttccgt tggcaggcag tgacgttcga caacccgatt
      601 ggaccaacaa caacacgcgg cgagaacccg gtaagcttcg agattgaact ccggtttgac
      661 ggaacgatca cattgcgtta tggcgacggc aatcagaaaa cattgccggt aatcggcatc
      721 ggcggtgggt cgcctgaacc ttatatctcg acatcgcatt cgtccgaaag tagtttgaaa
      781 gatctaacta atgcggcgac tgtcgtcttc gctcgtcgca agccaatcca acgcggcgtg
      841 ctgactgtgg cctcaatcaa tcccgcgagt ggagtcaata tcactgtcag tccgaatgac
      901 tctacaggtg caggcgccgg cacgacgcag ttcactcgta cttacaattc aggcaccacg
      961 gtaactctca cggcgccggc tacagttaac ggaaacaagt tccaacattg gctgcgagat
     1021 ggacaggatt ggagtggaag tctcacgacg agcctggcaa tgcataccaa tctcaatgcg
     1081 acgatgacgg ctgtttatta caccccaccc gttttaaccg ttacatcggt taatccgagc
     1141 agcggtgttg agatatctat caatccaaac gacgtgaacg gagcgggaaa tgggaccaca
     1201 ccattcacac gtacgtacga tctgaatgtc caagcgaatt tagtcgcgcc cgccactgta
     1261 ggcacaagca cattttggaa atggcaactc gatggcgtcg actttacccc cggccggtca
     1321 ataggacttg cgatgagcaa ggcttacacc ttgacggctg tttacatcgc agcaacgccg
     1381 actccgactc cgactcccgt ccctggggcg ggcgcacagc ctatcgcgtt caccaaaccg
     1441 ggtaatggcg gaggcgcaga tgttttcctc acaaacatag acggcacaaa cgtcgtgaat
     1501 ctcacggatg cggcgggcga ggacacgcga ccggcctggt cgccagacgg ttcacggctc
     1561 gcgtatacct gcctccggca acctgatggc agtattggac caccgcaacg catctgtgtc
     1621 cgcaacgccg atggaaccgg attagtcgtg atgtctaagt cgcttcaaaa cgaggctggc
     1681 ccgaactggt cgcgcgatgg aaatcaaata ggttttacta ccagccccgg tctccagagt
     1741 ac
//
LOCUS       DASTWE010000606         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_760170, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000606 DASTWE010000000
VERSION     DASTWE010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760170"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1649
                     /gene="uvrC"
                     /locus_tag="VFT30_06320"
     CDS             <1..>1649
                     /gene="uvrC"
                     /locus_tag="VFT30_06320"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5092280.1"
                     /translation="DHNPKTSLLVSHITDVETMVTRSELEALILESNLVKRHKPRFNI
                     VLRDDKQYPYVRLPIKDDFPRLSIVRRVQKDGALYYGPYTPANALRETLKVIKHVFPL
                     ATCTIDIDGTADRACIEFEIKRCMAPCTGNQSKEEYHQIVKQVRQFLEGRDRELLDDL
                     RARMEAAAEQEEFEEAARLRDRLFKIERMLEKQRITQTSATDQDVIGLARQGSAVDLQ
                     ILFVRGGLLIGRKDFFWPQSAEVSDEELVHSAIEQFYNKDGQPPREVLVPIDLEDATL
                     IQQWLSDKRGESVRVRSPERGAKHQLVLLAEENAAAAVADHLRDEELDRQAGEELKRW
                     LRLERAPRRIEGFDISNTMGDQSVASMVVWEDGQMKKADYRRFKIRTVTGANDFASMK
                     EVVTRRYGREENLAQPDLILIDGGLGQLAAALEGLKEVGQQGVPILGLAKARGDKEER
                     VFLAGRKNPIVLKPQSPSTHLLQRVRDEAHRFAITFHRKLRGKSLVGSRLDQVIGIGE
                     IRRNQLLEKFGSLDKLSSASDEALREAGLNAKTVVDLRRALG"
BASE COUNT          386 a          467 c          488 g          308 t
ORIGIN      
        1 gaccataacc ccaagaccag tcttctcgtc agccacatca ccgacgtaga gacgatggtg
       61 acacgatccg agctcgaagc cctgattctt gagagcaacc tggtcaaacg ccacaaaccc
      121 cgcttcaaca tcgtgttgcg ggacgacaag caatatccct atgtgcggct gcccatcaaa
      181 gacgattttc cccgactctc gatcgtgcgc cgcgtgcaaa aagacggcgc cctctactac
      241 ggcccttaca caccggcgaa cgcactgcgg gagacgttga aagtcatcaa acacgtcttt
      301 ccccttgcca cctgcacgat cgatatcgac ggaaccgccg accgcgcctg catcgaattt
      361 gagattaagc ggtgcatggc gccctgcacc ggcaatcagt cgaaggaaga gtatcatcag
      421 attgtcaaac aggtccgtca atttctggaa ggccgggatc gcgagttact ggacgacctc
      481 cgtgcccgca tggaagcggc tgcggagcag gaagagttcg aggaagcggc ccgactgcgg
      541 gaccgcctct tcaagatcga acggatgtta gaaaagcagc ggatcacgca gacctcggct
      601 accgatcagg acgtgattgg cttagccaga cagggttcgg cagtggacct ccagattctg
      661 ttcgtgcgcg gcggcttgct gattggacgg aaagatttct tctggcctca gtcggccgag
      721 gtgtccgatg aggagttggt gcattccgct atcgagcagt tctacaacaa ggacgggcag
      781 cctccgaggg aagtccttgt cccgatcgat ctcgaagacg ccacactgat tcaacaatgg
      841 ctgtccgaca agcgagggga atccgttcgc gtacgttcgc cggagagagg cgccaagcat
      901 cagctcgtcc ttttagccga agagaatgcc gctgctgctg tcgccgatca tttgcgcgat
      961 gaggaactcg accggcaagc cggtgaggaa ctgaaacgtt ggctgcgact ggaacgcgct
     1021 ccacgtcgga ttgaagggtt cgacatctcc aatacgatgg gcgatcaatc agtcgcctct
     1081 atggtcgtct gggaggacgg ccagatgaag aaggcggact atcgacggtt caaaatccga
     1141 acggtcacgg gagccaatga ctttgcaagc atgaaagagg tggtcacgcg gcgctatggg
     1201 cgggaggaga acctggctca gccggatctg attctcatcg acggcggact aggccaactg
     1261 gctgccgcgc tggaggggct caaagaggtc ggacaacagg gtgtgccgat ccttgggctg
     1321 gccaaggcac gcggcgacaa agaggagcgt gtcttcctgg cgggaagaaa aaacccgatc
     1381 gtgctcaagc cgcaatctcc ctccacacac ctgctccaac gcgttcgcga tgaagctcat
     1441 cgttttgcga tcacgttcca tcgaaagctg cgaggcaaat cactggtcgg ctcgagactg
     1501 gatcaggtca tcggaatcgg tgagattcgg cgcaatcaac tcctagagaa attcggcagt
     1561 ctcgacaaac tgtcttcggc cagcgacgaa gctctacgtg aggccggtct caacgcaaag
     1621 acggtcgtgg acctccgtag ggcgcttgg
//
LOCUS       DASTWE010000607         1344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1050825, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000607 DASTWE010000000
VERSION     DASTWE010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050825"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1344
                     /locus_tag="VFT30_06325"
     CDS             <1..>1344
                     /locus_tag="VFT30_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092281.1"
                     /translation="AVAPANPGTIAVARHLGNVSPPQAGVAIFDNGVQRPNVGPSNLS
                     GADYLAFSASASKLYGNDPLGGIQTFTLDASGVTLGSTVAPSVFGKIKFSNGLIFTSG
                     GHVVNPETGSVLGTFPNVITANASAFVPDTSVGRAYYLTFGQDSGTVNIKVFDINTFL
                     PLGSLNISGVGGNPTSLIRWGANGLAFRTDSQLFVIQTSLIPSAESIPTPTPTPTPTP
                     FPEPSPPAVAFIRQMTLSANDLIFNPATQKIYASVPSNEGSNGNSIAEIDPVLGSVTN
                     QVFVGSEPNILAPSDDGSTLYVGLDGAASIRRYNMQTHTAGQQFFLGRDNSFGPLGLG
                     GIAVSPGNPSTVAVALQHPNILPLQAGVAIYDDGVRRPKIGPGQPDGSDLLAFASPSL
                     LYGATAFGFGDLTKFSVDSSGVTVIGTAPFFAGAEITLANNLLYGSSGQVIDPSTG"
BASE COUNT          283 a          382 c          355 g          324 t
ORIGIN      
        1 gctgttgctc cggcgaatcc cggtacgatt gccgtagccc gacatctcgg gaacgtcagt
       61 ccgccgcaag caggtgtggc catcttcgat aatggtgtgc agcggcccaa tgttggacca
      121 agtaatctca gcggcgctga ctatctggct ttctccgcgt ctgcgtccaa gttatatgga
      181 aacgatccct taggaggcat ccagacgttc acgcttgatg cgtcgggtgt gaccctcggc
      241 tctacagtgg caccgagcgt gtttggaaag atcaaattca gtaatggcct gatcttcact
      301 tcaggtggtc acgttgttaa ccctgagacc ggttcagtat tgggcacgtt cccgaatgta
      361 atcacggcaa atgcgagtgc cttcgtgcct gatacttcag tcggacgcgc ctactatttg
      421 acgtttggtc aagactccgg aaccgtaaac atcaaagtct tcgacataaa tacttttctg
      481 ccgctggggt cgcttaacat aagtggagtt gggggaaatc cgacttcgct cattcggtgg
      541 ggtgcgaacg gactcgcgtt tcgcactgac agccaattat tcgtcatcca gacgtcactg
      601 attccgtcag cagaatcgat accgacgcct actccaactc ctacaccgac tccatttcca
      661 gagccgtctc ctccagccgt tgctttcatt agacagatga cgttgagtgc aaacgatctc
      721 atcttcaacc cggcaacaca aaagatttat gcgagtgtgc cgagcaatga aggcagcaac
      781 ggaaacagca tcgccgagat cgatccggta ctcggctccg taacaaacca ggtctttgtg
      841 ggaagcgagc caaacatcct ggcgccgtct gacgacggct caacacttta tgtcggcctt
      901 gatggtgcag cctcaattcg gcgttacaac atgcagactc ataccgcggg ccagcagttt
      961 ttcctggggc gagacaactc ctttggtcca ctcgggctcg gtggaattgc tgtgtctccc
     1021 ggtaatccgt ccacggtcgc ggtagccctc cagcacccga acatcttgcc attgcaagca
     1081 ggtgtcgcga tttacgatga tggtgtgcgg cgtccaaaaa ttggtccggg ccagccggac
     1141 ggcagcgacc ttcttgcatt tgcgtccccg tcactgcttt acggagctac tgctttcggt
     1201 ttcggcgatc tcaccaaatt ctctgttgat agctctgggg tgacagtgat cggtactgcg
     1261 ccgttcttcg ccggagcgga gatcactctc gccaacaatc ttctctatgg ttccagtggg
     1321 caggtgatcg accccagcac gggg
//
LOCUS       DASTWE010000608         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1600225, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000608 DASTWE010000000
VERSION     DASTWE010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600225"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1131
                     /gene="ftsH"
                     /locus_tag="VFT30_06330"
     CDS             <1..>1131
                     /gene="ftsH"
                     /locus_tag="VFT30_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5092282.1"
                     /translation="EVLEIIEFLKDPRKFQKLGGRIPKGVLVVGPPGTGKTLLAKAIA
                     GEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGL
                     GGGHDEREQTLNQLLVEMDGFDTTEGVILVAATNRPDVLDPALLRPGRFDRQVVVNRP
                     DLKGRSEILKVHTKKVPVAANVELEKIARGTPGFSGADLENLVNEAALWAARQNKKEV
                     ENIDFEMAKDKVMMGAERKSMILTEEEKRVTAYHEAGHALMAKLLPGTDPVHKVTIIP
                     RGRALGVTMQLPTDDRHNYSKEFLYNTLAILMGGRVAEELVFKHVTTGAGNDLERATD
                     LARKMVCEWGMSEKLGPLTFGQKEDSVFLGRDFTTKRDVSDQV"
BASE COUNT          279 a          244 c          345 g          263 t
ORIGIN      
        1 gaagtccttg aaatcattga attcttgaaa gatcctcgga agtttcagaa gctcggcgga
       61 cgcatcccca agggcgtact tgtcgtcggc cctccaggaa caggaaagac tttgctggcg
      121 aaagctattg cgggagaagc cggggtaccg ttcttcagca tcagcggttc tgactttgta
      181 gagatgtttg tcggtgtcgg agcttcacgg gtgcgggatt tgtttgagca agggaagaag
      241 catgccccgt gcattatttt catcgatgaa attgatgcgg ttggtcgctt gaggggtgca
      301 ggcctcggcg gtggccatga cgaacgggag caaacgctca atcagttgtt ggtggaaatg
      361 gacggcttcg atacaaccga aggagtcatt ttggtcgcag cgaccaatcg tcccgatgta
      421 cttgatcctg ccttgctgag acccggacgc tttgatcggc aggtggtggt gaatcgccct
      481 gacctcaaag gccgttcgga aattttgaag gtccacacga aaaaggttcc tgtcgccgcg
      541 aatgtggagt tggaaaagat tgctagggga acgcctgggt tttcaggcgc tgatttggaa
      601 aacctcgtga atgaagcggc attgtgggcg gctcgtcaga acaaaaagga agtcgagaac
      661 atcgactttg agatggccaa ggacaaggtc atgatgggcg ccgagcgcaa gagcatgatt
      721 cttacggagg aagaaaaacg agtgacggca tatcatgaag ccggccacgc gttgatggcg
      781 aagcttcttc ctggaaccga tcctgttcat aaagtgacga tcattcccag aggtcgggct
      841 ctaggtgtga cgatgcagct accgacagat gatcggcaca actattctaa agaatttctc
      901 tataatacct tggcgattct tatgggtgga cgtgttgccg aagaactcgt attcaagcat
      961 gttacaaccg gagccggtaa cgatcttgaa cgtgcgacgg accttgcccg gaagatggtg
     1021 tgcgaatggg gtatgagtga gaagttgggg cccttgacct ttgggcaaaa ggaagattcg
     1081 gtgtttctcg gacgagactt taccaccaaa cgagatgtca gtgatcaagt c
//
LOCUS       DASTWE010000609         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1600227, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000609 DASTWE010000000
VERSION     DASTWE010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600227"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFT30_06335"
     CDS             <1..325
                     /locus_tag="VFT30_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5092283.1"
                     /translation="SAIDVEKATAELDVLVNLEETSEGLSGDFEYNTDLFDASSIARL
                     VGHFTTILEQVSIRSDVTLKELEHVLAEADSQQQSRKQQELTQASYTKLKSIKRKIVV
                     HRQEK"
     gene            335..1420
                     /locus_tag="VFT30_06340"
     CDS             335..1420
                     /locus_tag="VFT30_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU5092284.1"
                     /translation="MKHSNLESQSLKKIKEVQRKAISLSEATLVEAKPLVAEKPLPLL
                     VQPAVKGVNLLTWAEEKREWIHTNMLRHGAILFRNFLVNGIEDFERCVAAMSSGALEY
                     RFRASPRTQVGANIYTATDYPADQSIFPHNEHAFSPVFPLQLFFYCVTPALEGGETPI
                     GDTRQVHRNIDPDIKRRFLEKGIMYVRNYGDGFGLPWKTVFQTEDRGEVEAYCRSVGI
                     DVEWKSSNRLRTRQVGPAVMRHPRTQEPIWFNHGTFFHVSTLEPTIREALLKEFDEAD
                     LPQNTYYGDGSPIEPDVLEHLRSTYEQAMVVFPWRQGDVVLLDNMLALHGRKPFSGPR
                     KILVAMAEACRGADLAWRPEKESSCQE"
     gene            1408..>3365
                     /locus_tag="VFT30_06345"
     CDS             1408..>3365
                     /locus_tag="VFT30_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5092285.1"
                     /translation="MSGINASEQTIQGYRLSPQQERLWLLGQGGEQAHYDARCVIRVE
                     GALDPAVLKKAIQQVVQRHEILRTTLQGLPGMKFPVQVIAETGAISYEEQDLTAATPA
                     EQKSCSEAFLCATPDETNDSLLRFNLVKLGSARYELHLSLSACCSDRATLKNLVHEIS
                     RMYQACLRGEYRSDDVPLQYADFAEWQQTLLEDETGETGRAHWRKQADSVAAHLQGRL
                     PFEHLPTEHISFTSKRLAVAVTQESVQRIDEMMKSHGVSGSVFLLACWCILLQRLTAQ
                     EDVLIGVACDGRTHEDMQGALGPFTKYVPVSIKVDSASRFSTFVLRLQQTVVEGQEWQ
                     EYFTWKDIQEVEKDLGEKHFPFCFEFDEQLPIEPVATPAFLIEYSRSFIDRFKVRFVC
                     ERKGDIIDAALEYDAVLYSDDVIACFAEQLATLVSHAACEPEMSICHMDMLSVVERHR
                     MLSTFNESGSVDGRNQCLHHLFETQVKFTPDSVAVVYEEQTLTYHELNRRADQLAHYL
                     RNRGVGPETAVGICMERSPELIVGLLAILKSGGAYVPIDPSYPKDRIVYMLEDSRAPL
                     LLTQRRFRDDLAGTSADVVNLDGEPHRFERDCGHPSLVQETPDRVAYIIYTSGSTGHP
                     KGVLVSHRNAVHSTLARHSYYNDPVSRF"
BASE COUNT          816 a          841 c          951 g          757 t
ORIGIN      
        1 gagcgctatc gatgttgaga aggccacagc ggaactggat gtattagtca atctggaaga
       61 aacgtcggag gggctaagcg gagattttga atacaatacg gacctgttcg acgcatccag
      121 catagcccga ctcgtgggac attttacgac cattttggaa caggtatcta ttaggtctga
      181 cgttactctg aaagagctgg aacacgtgct ggctgaagca gacagtcagc agcaaagcag
      241 aaaacaacag gagctcactc aggcgagcta tacgaaattg aaaagcatca aacgcaaaat
      301 agtcgtccat cgacaggaaa agtgaggggc agccatgaag cactcgaatc ttgaaagtca
      361 aagcttaaag aaaattaagg aagttcaacg aaaggccatc agtctctctg aagcgaccct
      421 ggtggaggcg aaaccgctcg tcgccgagaa gccgcttccg ttattggtcc aacccgctgt
      481 caagggagtc aatctactca cctgggctga agaaaagcgg gaatggattc atacgaacat
      541 gcttcgccat ggcgccatcc tgtttcgaaa ttttctcgtc aacggaatcg aggactttga
      601 gcgctgcgtc gcggcgatgt ccagtggggc tctggagtac cggtttcgag cctctccgcg
      661 cacgcaggtg ggggcgaata tctataccgc cactgattat ccagccgatc aaagcatctt
      721 tccccacaat gaacatgcgt tctctccggt ctttccgctg cagttgttct tttattgcgt
      781 cactccggct ctggagggcg gagaaacccc gatcggcgat acacgccaag tccaccgcaa
      841 catcgaccct gacatcaaac gacggtttct cgagaaaggg attatgtatg tgcgcaacta
      901 cggcgatgga ttcggccttc cctggaaaac cgtctttcag acggaagata gaggtgaagt
      961 cgaggcgtac tgccgaagtg tcggcatcga tgtggaatgg aaaagctcaa atcgtttgcg
     1021 gacgagacaa gtcgggccgg ctgtcatgcg gcatccacgc acgcaggaac cgatttggtt
     1081 caatcacggt acgtttttcc atgtctcgac actggagccc acgatccgag aagcgctgtt
     1141 aaaagagttt gacgaggcgg atcttccgca aaacacctac tacggcgatg ggagtccgat
     1201 cgaaccggat gtgctggaac atctccgttc gacctatgaa caagccatgg tggtgtttcc
     1261 atggcggcaa ggggacgtgg tcttgctgga caatatgctg gcgctccatg gccgtaagcc
     1321 gttcagtggg ccacgcaaaa ttttggtggc aatggcggag gcatgccgcg gcgccgacct
     1381 cgcatggagg ccggagaagg agtcgtcatg tcaggaataa acgcgtcgga gcaaacgatt
     1441 cagggatacc gcctctctcc gcaacaagaa cgattatggt tattgggaca gggcggagaa
     1501 caggcacact atgatgcccg ctgcgtgatc agggttgagg gcgcactgga tccggcagtc
     1561 ttgaaaaagg ctatccagca ggtcgtgcaa cgtcatgaaa tcctacgcac gacactgcaa
     1621 ggtctccctg gcatgaagtt tcccgtgcag gtcatcgcgg agacaggcgc catttcatac
     1681 gaagagcagg atctgaccgc ggctacgcct gccgaacaaa aatcctgcag tgaagccttc
     1741 ttatgcgcca cgcctgacga gacaaacgat tccttgctac ggttcaactt ggtgaaactc
     1801 gggtcggcaa ggtacgagtt gcatctcagc ttatccgcat gttgctcaga cagggcaaca
     1861 ttgaagaatc ttgtgcatga aatcagtcgc atgtaccagg catgtctgcg gggcgaatat
     1921 agatctgacg atgttccgct gcagtatgcg gattttgccg aatggcagca gactctccta
     1981 gaggatgaga cgggagagac agggcgcgct cattggcgga agcaagccga ttctgtggct
     2041 gcccatcttc aggggagatt gccctttgaa catctgccta ccgaacatat atcgttcacg
     2101 tcgaagcgac tcgcggtggc cgtgacacag gagtccgtcc agcgaatcga tgaaatgatg
     2161 aagtctcacg gggtttcagg atcggtgttt ttgttggcat gctggtgcat tcttctccag
     2221 cgcctgactg cgcaggaaga cgtgcttatt ggtgtcgcat gcgatggccg gactcacgaa
     2281 gacatgcagg gagcactcgg gccgtttaca aaatatgtgc cggtatccat taaagtggat
     2341 agcgcatccc gattcagcac gttcgtgctg cgtcttcagc agacggtcgt tgaaggtcaa
     2401 gaatggcaag agtatttcac ctggaaagat atccaggagg tagaaaaaga cctaggcgaa
     2461 aagcacttcc cattttgttt tgagtttgac gaacagcttc ccatagagcc ggtcgccaca
     2521 ccggcttttc tgatcgaata cagtcgttcc tttatcgatc gattcaaggt ccgattcgtg
     2581 tgtgaacgaa aaggagatat catcgatgcg gcgttggaat acgacgcggt gctctatagc
     2641 gacgacgtca tcgcctgctt cgcagaacaa ttggcaacgt tagtgagtca tgcggcatgt
     2701 gagccggaaa tgtccatatg ccatatggac atgttgagcg tcgtggagcg ccaccgaatg
     2761 ctttccactt tcaacgagtc gggttcagtc gacggtcgaa atcaatgcct gcaccatctt
     2821 ttcgagacgc aggtcaagtt cacaccggac agtgtcgccg tggtgtatga ggaacagacc
     2881 ctcacctatc acgaattgaa tcggcgggcc gatcaattgg cgcattattt gagaaatcgc
     2941 ggggtcggcc cggagacggc ggtgggtatt tgcatggaac gctcgccgga attgattgtt
     3001 gggttgctgg ctattctcaa atcgggcggg gcctatgtgc cgatcgatcc atcctatccc
     3061 aaggaccgta ttgtctatat gcttgaggac tcgcgggctc ctcttttgtt gactcaacga
     3121 cggttccgtg atgatttggc ggggacttct gccgacgttg taaatcttga tggagagccc
     3181 catagatttg agagggattg cggacatccg tctttggttc aggagacgcc agatcgcgta
     3241 gcctatatca tctacacgtc cggctcgact ggacatccca aaggtgtcct ggtgagccac
     3301 cgcaatgcgg tgcactccac gcttgcacgg cacagttatt acaacgatcc tgtttctcgc
     3361 ttctt
//
LOCUS       DASTWE010000610         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_824636, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000610 DASTWE010000000
VERSION     DASTWE010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824636"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VFT30_06350"
     CDS             complement(<1..269)
                     /locus_tag="VFT30_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092286.1"
                     /translation="MGHYWQEINALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADTQVMQAPNYISFLAAGVVVMTVLNNGLAGGVDLLFDKENG"
     gene            complement(279..1250)
                     /locus_tag="VFT30_06355"
     CDS             complement(279..1250)
                     /locus_tag="VFT30_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5092287.1"
                     /translation="MDRTIAIDVSRLCKTYDSHKAVDDLSFQVYAGEIFGLLGPNGAG
                     KSTTLRTLITLLHPTSGTATVMGYDTVRDADRVRTVIGYVPQERAIDRFLTGREHLQL
                     LGALYHLTKEEAAKRIGELLKLVELEAHADRPAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWEYVRMLKTRGMTVVMTTNYLDEADRLCDRLAIIDGGKIKTL
                     GTPAELKIALGGDIVSLTLKETSRIQSLASDLTDRPAIKTVRATTKGLDIRVESPEKA
                     LPTILESANRLGCDIEFIQYNRPRLDDVFIAHTGRAITESPSEVESA"
     gene            complement(1250..1939)
                     /locus_tag="VFT30_06360"
     CDS             complement(1250..1939)
                     /locus_tag="VFT30_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5092288.1"
                     /translation="MQTDRERGPSDRSPSKHAEPFPIPNRLPVFPLPNVVFFPKTYLP
                     LHIFEPRYRRMIADVTMGGQCIAMALLKEGWEPDYYGNPAIYPALCIGRVVSVQPLSD
                     GRSNILLQGLERCEISEEHFDKPYREATIRVTPMRSDEGLTKGVRRALIDVLGRYLQA
                     REDSAAWQGFFREEVSDEVLVNTLSTYLDCTPLEKQFLLEADGLHQRARRLNDLVQFM
                     LHVPHGTKGWE"
     gene            complement(1971..>2195)
                     /locus_tag="VFT30_06365"
     CDS             complement(1971..>2195)
                     /locus_tag="VFT30_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092289.1"
                     /translation="CSRYQAEVEGQDSHDENNSLNGVHHPPRFMVKQRIGLEPDVLSC
                     LYHVRSRSNKRQSQSDVSHLPRAGQSAARR"
BASE COUNT          466 a          629 c          610 g          490 t
ORIGIN      
        1 ccgttttcct tatcgaacag cagatcgacc cccccggcca agccgttgtt cagaaccgtc
       61 atgacgacga caccggccgc gaggaagctg atgtagttgg gcgcctgcat gacctgcgtg
      121 tccgcggccc gttgaaacaa gtttccgaag aagatgagcc agaacagcat cggctgcacc
      181 aaggtgaaca gcatgctgaa tttttcccgg ctcaaccgtc gaacccaccg catcgtcagc
      241 gcgttgatct cttgccaata gtgccccacg tgactccttt atgccgactc gacttcggaa
      301 ggcgattcgg tgatggctct ccctgtatgc gcgataaaca catcgtctaa gcgcggacgg
      361 ttgtattgaa taaactcgat atcgcaacct aaccggttgg ccgattcaag aatggtgggt
      421 aaggcctttt cgggcgattc tactctgata tccaagccct tggtggtcgc tctgacggtc
      481 ttgatcgccg gacgatcggt gaggtccgac gccagcgact ggatccggct cgtctctttc
      541 agagtgaggg acacaatatc cccgccaagg gcgatcttga gctcagccgg ggttcccagc
      601 gttttgatct tgccgccgtc gataatggcg agtcgatcgc agagtcgatc ggcctcatcc
      661 agatagttcg tcgtcatcac gaccgtcatc cctcgggtct tgagcatccg aacgtattcc
      721 caaatccgga gacggctttg cacgtcgagg ccgagcgtgg gctcgtcgag aaacaagatc
      781 ttgggatctg gcagcaaccc gcaggcaatg tcgagcttgc gcttcatccc accggaatag
      841 gtcttggcgg gccggtccgc atgagcctcc agctcgacca atttgagcag ctcgccgatg
      901 cgtttcgccg cttcttcttt cgtcaggtga tagagcgcac cgaggagttg caggtgttca
      961 cgcccagtca gaaaccggtc gatcgcccgt tcttgaggca cgtatccgat cacagtccgg
     1021 acccgatccg catcgcgcac tgtatcgtat cccatgacgg tcgccgtgcc cgatgtcggg
     1081 tgcagcaacg tgatgagtgt acgaagagtg gtgcttttcc ctgctccgtt cggccccagc
     1141 agaccaaaaa tttcccccgc gtagacctgg aacgagagat catcgacagc tttatggctg
     1201 tcatacgttt tgcacagacg gctaacgtcg attgcgatgg tccgatccat tactcccagc
     1261 ccttcgtccc atgaggtacg tgcagcatga attgaaccag atcgttcaat cgacgagcac
     1321 gctggtggag tccgtccgct tccagcaaga attgtttttc caagggtgtg cagtcgaggt
     1381 aggtggagag ggtattgacc aagacttcgt cgctgacttc ttcgcggaag aacccctgcc
     1441 acgccgcgct atcctccctc gcctggaggt atcgaccgag cacgtcgatc agcgcgcgcc
     1501 gaacgccttt cgtcaatccc tcgtctgagc gcatgggcgt gacgcgaatc gtcgcttcgc
     1561 gatacggctt gtcgaagtgt tcttcggaaa tctcgcaccg ttcgagtcct tgcagcagga
     1621 tgttggagcg accgtccgac aagggttgca cactcacgac ccgtccgata cagagcgcgg
     1681 gatagatcgc cggattacca tagtagtcgg gctcccaccc ttctttaagg agtgccatgg
     1741 caatacattg accgcccatc gtcacatcgg cgatcatccg acgatagcgc ggttcaaaaa
     1801 tgtgaagcgg caggtacgtc ttggggaaaa aaacgacgtt aggtaatggg aacaccggaa
     1861 gacgattggg aattggaaaa ggttcggcat gtttggacgg actccggtcg gacggtcccc
     1921 gttcacgatc agtctgcatt gctcacgata gctgagacgt gtgaaaattg tcaacgccgt
     1981 gccgctgact gcccagccct gggtaaatgc gaaacatcgc tctgagactg tcgtttgttc
     2041 gatcgagagc gtacatgata taaacagctc aagacatcag gctctagtcc gatgcgttgc
     2101 ttgaccatga aacgaggcgg atgatgcacg ccgttcaacg agttattctc atcgtgactg
     2161 tcctggcctt cgacctcagc ttggtatctg gagca
//
LOCUS       DASTWE010000611         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1050855, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000611 DASTWE010000000
VERSION     DASTWE010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050855"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..886
                     /locus_tag="VFT30_06370"
     CDS             275..886
                     /locus_tag="VFT30_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5092290.1"
                     /translation="MPIILAVNGIVETYPIKPVGPRNGAESPGEQEHHGQHSSSLDRS
                     ALAAQTAYQQQSIERSHLKPAILARDLMSAPVLSLPSDSTLLDAWASMSHKGFHHIPV
                     TSMHDTLVGMVSYRDLLRHVPELITAADKRQAARRRLAEIMTSRVISATPVTEIREIA
                     RAMLDERIHAIPILDYNRHLVGILTTRDLLQGIANHGPLELWT"
     gene            complement(1336..>1774)
                     /locus_tag="VFT30_06375"
     CDS             complement(1336..>1774)
                     /locus_tag="VFT30_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092291.1"
                     /translation="EAGHYQSGGIMISSSKGVQTDRVLLLGLAILLQTGCASSAGDKI
                     MRPPCDISPGQNVMQAQQDAMRKSHMIFGEVLRVDGTTYVLKDDDGKEVSVKIDESTE
                     KPPINKGDRIAANVDNQNLALWIRANRGTDRRTEHASADCNPN"
BASE COUNT          416 a          492 c          438 g          428 t
ORIGIN      
        1 ggacgtgcag gctttggtaa tgtgcacctt tcatctattg tccatgcgtc ggctatcaac
       61 gctcaccgag tcgcacaacg ctccttatag catagggttc aaaacggaag caaccgattt
      121 ggaacgcgag gttgcgataa ccatagcaac tgatccacct cacgccgcca agggacagaa
      181 gtgccaccgc aggaacgaca ccagagggca tttacaacct cctccgctta agtatttccc
      241 gaatattccg atacggcagc tgaggaactg cagcatgccc atcattctgg cggtcaatgg
      301 aatcgtggag acgtatccga tcaagccggt tggcccacgg aatggcgctg aatcccctgg
      361 agagcaggag catcacggcc aacattcatc ttcgttggat cgctcagcct tggcggcgca
      421 aacggcttat caacagcaaa gcatcgagcg gtcccacctg aaacctgcaa tcctggctcg
      481 tgatttgatg agtgctccgg ttctctctct tccatccgac agcaccttgc tcgacgcctg
      541 ggcgagtatg tcacacaaag gattccacca tatccccgtc acgtcgatgc atgacaccct
      601 cgtcgggatg gtgtcgtatc gtgacctact ccgccatgtg ccggaactta tcacggcagc
      661 cgataagcga caggcagcca ggcggcgatt ggctgaaatc atgacttccc gggtcatatc
      721 tgcaacaccc gtgacagaaa tccgcgaaat tgcccgcgcc atgttggatg agcggattca
      781 cgctattccg atcttggatt acaatcgcca tctcgtcggc atccttacca cccgagatct
      841 gctgcagggc atcgcgaacc acggccctct tgaactttgg acctgacttc cacttccgac
      901 gaaacgttcc gattcgagat cctgaagaaa gtgctcattc gatcggctgt cttgacggac
      961 gaatcaaaga gttgagcgag acacgcgttc aagaacgtga tcggaaggag attctgccaa
     1021 tgagagagta aagaaggaca gagcatattt aaacgagcgt aagagtactt ccttccttaa
     1081 gaaaatctcg cggtgcagcg gcgcctaaag cggctggatg acctagtccg cctaggcatt
     1141 ttaagcatct tggagggtgc taccgttcca gctccgaagt cgggaaggta acacacaggg
     1201 gttgctgaat tagttcaatg gtcttttcgc atgtctcagc tccatgccct gtgctttgaa
     1261 tgtctctaac gtttgttccg accggctttc tcacaacgtg cagaggtccc cgccggtcga
     1321 agaacctacg gcaggttaat tcggattgca gtcagccgag gcatgctcag tgcgacgatc
     1381 cgtccctctg tttgcgcgaa tccacagggc gaggttctga ttgtctacat ttgccgcaat
     1441 gcggtctcct ttattaatag gaggtttctc cgtgctttcg tcgatcttca cgctcacttc
     1501 tttaccatcg tcatctttca ggacatacgt cgtaccgtct acacgtagta cttcaccaaa
     1561 aatcatatgg cttttacgca tggcatcttg ttgggcctgc atcacattct gtccaggtga
     1621 tatatcgcac ggtggtctca taattttgtc tccagcagat gatgcacaac cagtctgtaa
     1681 caggatggct agtcccagca acaatactct gtcagtttga acaccttttg aagaggagat
     1741 catgatgcct cctgattgat agtgaccggc ttcc
//
LOCUS       DASTWE010000612         1635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1179941, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000612 DASTWE010000000
VERSION     DASTWE010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179941"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFT30_06380"
     CDS             <1..294
                     /locus_tag="VFT30_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092292.1"
                     /translation="MDRSMIVTAWEQHCADGWPQFSSPHQGQLMTIDTVISGCVIYYL
                     DSSGSLDDQRLAIVKDCLGDLDELTEALDTESQAYFYRLRELGAMLLGDEPRS"
     gene            431..961
                     /locus_tag="VFT30_06385"
     CDS             431..961
                     /locus_tag="VFT30_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092293.1"
                     /translation="MTKDSGTRMAIILLVSILATVASAHAEPYELSKNDVMEPKAISS
                     GEISLFGVKLGDSESKVVEALVNEKISGVKAEQEATFIFLLDQRKPTGPMAGVRIQDG
                     KVDLLFINNRFAYKTRGIFRNVLNSESPDDIRKLLGQEEYGDENVMGAVMAYDKQGFQ
                     VNYLGKDINIEFAAPR"
     gene            complement(1081..>1635)
                     /locus_tag="VFT30_06390"
     CDS             complement(1081..>1635)
                     /locus_tag="VFT30_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092294.1"
                     /translation="PKTHLRLKIGEVFRLGDLLKAMMMVSANDACLAAVEHVGGDEEQ
                     FVMLMNIKAVALGLSDTHFSNGCGFDGPDHYSTAEDLAKLSEVAMRNAVFRELVKEER
                     EIITPVSGYRAYVLHNTNRLLGRIPGVEGVKTGFTSKAGRCLIAKVSQNGSDLLLVIL
                     NSNRRWNTAKSLIDYGFRLTNTVQ"
BASE COUNT          415 a          403 c          420 g          397 t
ORIGIN      
        1 atggatcgaa gcatgattgt aacagcgtgg gaacagcatt gtgccgatgg ttggccccag
       61 ttttcgagtc cacatcaagg acaattgatg acgatcgaca cggtcattag cggctgtgtg
      121 atctactacc tcgatagttc cggaagcctc gacgaccaac gccttgccat cgtgaaagac
      181 tgtttgggag atctcgatga actgacagaa gcattggaca ccgagtccca agcctacttt
      241 taccgacttc gtgaactcgg ggcaatgttg ttgggcgatg agccgcggtc atgagcaagg
      301 gaccgctcgt acatcccatt ccttgcaggt ctcgcagctc ttggttacaa tgtgttatat
      361 gcttatctgc actctgctga ttcacctatc ctgaccaagt cgccaactct tgcgaaagaa
      421 acgctgcacc atgacaaaag acagtgggac gcggatggca atcatcctac tggtaagtat
      481 cctcgcaaca gttgcctcag ctcatgccga gccgtatgaa ttaagcaaga acgatgttat
      541 ggaaccgaag gccatttcga gcggtgagat ctcgctgttc ggcgtcaaac tcggcgactc
      601 ggaatccaaa gtcgtcgaag ctctcgtgaa tgaaaagatt tccggagtta aggctgaaca
      661 ggaagcgact ttcatcttcc tgctcgatca acgaaaaccg actggtccaa tggccggggt
      721 gcgtatacag gacggtaagg tcgatctcct tttcatcaat aatcggttcg cgtataagac
      781 cagaggtatt tttcgtaacg tactcaacag cgagagtcct gatgacatcc gaaagttatt
      841 aggccaggag gagtatggtg acgaaaacgt catgggcgcc gtcatggcct acgacaagca
      901 gggtttccag gtaaattacc ttggtaagga catcaacatc gagtttgcgg ccccacgata
      961 atgcggtcca gatgtggcgt catccatcgg gcgctgtact taaaaatgaa ttggctcgat
     1021 ggatactatc gtaatcggtg gaaggggtaa ggccaagaat gagaagagga gtcttctatg
     1081 tcattggacg gtattcgtga gacgaaaccc ataatcgatt aggcttttcg cggtattcca
     1141 ccggcggtta gagttcagga tcacgaggag aagatcgctg ccattttgag agactttagc
     1201 aatcaggcat cggcctgctt tggaggtaaa tccggttttc actccttcca cgccgggaat
     1261 tcggcctagt aaacgattcg tgttatgcag gacataggcg cgatatccac tgacaggtgt
     1321 gataatctcc cgttcctctt tgacgagttc tcggaacact gcgtttcgca tcgccacctc
     1381 actgagtttc gcgagatctt cagcagttga gtaatggtcg ggaccgtcga aaccgcaccc
     1441 gttgctgaaa tgcgtgtccg ataatccaag tgcaacagct ttgatgttca tcaacatcac
     1501 gaactgctct tcatcacctc cgacatgctc aacagccgct aggcaggcgt cattggctga
     1561 caccatcatc atggccttga gcaaatcacc aagacgaaac acttccccaa tctttagtcg
     1621 taagtgagtc tttgg
//
LOCUS       DASTWE010000613         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1083149, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000613 DASTWE010000000
VERSION     DASTWE010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083149"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VFT30_06395"
     CDS             <1..590
                     /locus_tag="VFT30_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5092295.1"
                     /translation="AENPPARLSRDAPVQKSNFMAYNIHAVWQQEVLAAVERSRKYIN
                     KYMGGLLTAENVDDADVIIIASGSAAAQSREAVRLCKEKGMKVGLIKVRSLRPFPTKE
                     LRQLCSKAKLIVVPEFNYVGWLAKEVATAIYGFSNAKIIGGPRVYGGQSMPVELIVDE
                     VESGVSGKKSTNVAMSQIMGGVVNADEVAHFMRSI"
     gene            complement(737..985)
                     /locus_tag="VFT30_06400"
     CDS             complement(737..985)
                     /locus_tag="VFT30_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092296.1"
                     /translation="MTDEILKAYKEVESAVERYIRLLHDHVAMLQNIEPPGSDKVVRL
                     TAGSKAMTDSAAIYLSYAKYVAYGMPASEEMVEDEIQG"
     gene            1024..>2068
                     /locus_tag="VFT30_06405"
     CDS             1024..>2068
                     /locus_tag="VFT30_06405"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEU5092297.1"
                     /translation="MRRLNARVSPLLLDAIKELVGREGFSQEILAQAVAELSRLFTKD
                     RNHLVRTYLDDELLGGAYLQYFLPVNLAKIQLLLKEMLVPELVEGFSVLDIGSGPGTG
                     ALAVLDWWHQEKLPSALSVTAVDSSSRALRQAGQLWGQYCRAANTQEAALQTYEKDLK
                     RQAWLEQVRQKGPFDLIILANCLNEIFAETGDPIMARTAFVGGSLALLAPHGTMMIVE
                     PALRETSRALHQVRDRLLQERRCTIYSPCLHEENCPALTKPDDWCHEERMWEPPVSIQ
                     EIDEEIGFIKDALKFSYLLLRKDGKTIVDRRPDVYRVVSELRELKGEKRAWLCNEEGR
                     QEVGRQDRLSSPQN"
BASE COUNT          480 a          510 c          592 g          486 t
ORIGIN      
        1 atgcggagaa tccccccgct cgtctgtccc gcgacgcccc cgttcagaag tcgaacttca
       61 tggcttacaa cattcacgcc gtgtggcagc aagaagtctt ggcggcggtt gaacgctccc
      121 gcaagtacat caataagtac atgggtggct tgctgacagc ggagaacgtc gatgatgctg
      181 acgtaatcat cattgcgtct ggcagcgccg ccgcgcagtc gcgcgaagcg gttcgtcttt
      241 gcaaggaaaa gggcatgaag gtcggattga tcaaagtccg ctccctccgt ccattcccga
      301 cgaaggaact ccgtcagttg tgcagcaagg ccaagctgat cgtggtccca gaattcaact
      361 atgtcggttg gttggcgaag gaagtagcta ccgccatcta cggtttctcc aacgcaaaaa
      421 ttatcggcgg tccgcgcgta tatggcggtc agtcgatgcc ggtagaactg attgtggatg
      481 aagtcgaatc cggtgtaagc ggcaagaaat ccaccaacgt cgcgatgtcg cagatcatgg
      541 gcggggtcgt caatgctgac gaagttgctc acttcatgcg cagcatctga gtcgacgttt
      601 ttaggtaatg aaagagcctg tccaggaaac cgggcaggct attttgcttt taggtgcgcc
      661 gggtggcttg tgattcgtga agcggatctc gcaagcattc aacgaacgag tgcttcacga
      721 gatacgagcg acgtcgttat ccttggattt catcctcgac catttcctcg ctcgccggca
      781 tgccgtaagc tacatacttt gcataggaaa ggtagatcgc cgcactgtcc gtcatggcct
      841 tcgagccggc agtcagtcgg acgaccttgt cggagccggg gggttctatg ttctgcaaca
      901 tcgcgacatg gtcgtgcagg agcctgatat atcgttccac agccgattcg acttctttat
      961 aagctttcaa aatttcatct gtcatggttg tcctccataa taggccgagc atacactagg
     1021 cccatgcgac gcctcaacgc cagggtttca ccgttactcc ttgacgccat taaggaactt
     1081 gttgggaggg aaggattttc acaagagata ttggcgcagg ctgtggcgga attgtccagg
     1141 ctctttacca aagatcggaa tcatttagtc cgaacatatt tagacgacga gctcttgggg
     1201 ggtgcgtacc ttcagtattt cctgccggtc aatcttgcca aaatccagtt gttgctcaag
     1261 gagatgctgg ttcccgaact ggttgaggga ttttccgttc tcgacattgg atcggggcca
     1321 gggactggag cattggcagt tctcgactgg tggcatcagg aaaagttgcc cagtgcatta
     1381 tcagtgaccg ctgtcgatag ttcgtcgaga gctctcagac aagccggaca gttgtggggt
     1441 cagtactgtc gggcagccaa tactcaagaa gcggctttgc aaacgtatga gaaagatttg
     1501 aaacgacaag cctggttgga acaggttcga cagaaggggc cgtttgatct catcattctt
     1561 gccaactgct tgaatgagat ctttgcagag acaggcgatc ccatcatggc gcgaactgcc
     1621 tttgtaggcg gatccctagc gttactggca ccgcacggca cgatgatgat cgtggagccg
     1681 gccttacgag aaacctcccg agcactgcat caggtgcgcg atcgattgct ccaagagagg
     1741 cgttgcacta tttatagtcc ttgtcttcat gaggagaatt gtccagcact aacgaagcca
     1801 gacgattggt gtcatgaaga gcggatgtgg gagccgccgg tgtcgattca agaaatcgat
     1861 gaagaaattg ggttcatcaa ggatgcgctc aagttttcct atctattgct gcgcaaagac
     1921 gggaagacca tcgtggaccg gcgaccagac gtctatcgag tcgtgagcga gttgcgtgag
     1981 ttaaaagggg agaagcgagc ctggctctgt aatgaggagg gccggcaaga agtcggacgg
     2041 caagatcgcc tttcgtctcc tcaaaacg
//
LOCUS       DASTWE010000614         1358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1147742, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000614 DASTWE010000000
VERSION     DASTWE010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147742"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..>1358
                     /locus_tag="VFT30_06410"
     CDS             254..>1358
                     /locus_tag="VFT30_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5092298.1"
                     /translation="MDIAILLGLIVLSAVISTAEIGFFAVNETRLRALAQNQNSRAKM
                     ALHLRSDPQKLLATILVGDRLVGTAIPMYATFLTLNTYGADRTFFDDTIAVVVGLLTF
                     VLLVSVDVVPKTLAAKFAVPVTLNMAYPVYGVQLVLKPILALMVPFIHRLTGGKGLTL
                     PLVTEEELKIMLDEGGKTGAIEVEKVKMIKNVFQLKDITAEDAMTPRLYVFSLDGNLR
                     LKEAQELLYNSKYSRIPVYDGTLDNITGILYKSRALTELAKNQTDAKLKEIAYPPIFV
                     PAGKTADDLMKQFQQEKRHMAVVVNEFGGVMGIVTLEDLLEEVVGEIMDETDITEELI
                     KRLGKNHILVHGRTEVRKVNDFLKVDLGDEAVTI"
BASE COUNT          316 a          369 c          359 g          314 t
ORIGIN      
        1 gtgtttcggg acgacaatta agcgtgatcc acaagccttt gattttatga tcacttttgt
       61 tgaagtgctt gccccctaac cttgcctatg atagatgtac tcctcatgaa actcttagtc
      121 ccccccaaag tctttcgcat cgatctcggt atctccgcca tgtgtctcgc ctgcccgaga
      181 gccggcagcg gctttctggc ccccatttgc cagacgactg tgcaagtgac ccgtccctaa
      241 acctggaagc tctatggata tcgccattct gctgggcctt attgttcttt ctgctgtaat
      301 ttcaactgcc gaaatcgggt tttttgcggt caatgagacc agactgcgtg ccttggctca
      361 aaaccagaac agccgggcca agatggccct ccacctccgt agtgatcccc agaaactact
      421 ggctacgatc ctggtcggcg atcgactcgt gggaaccgcc attccgatgt atgcgacatt
      481 cctcacctta aatacctatg gggccgaccg gacgttcttt gatgatacga ttgccgtcgt
      541 ggtcgggctt ctgacgttcg tgcttctggt gtcggtcgac gtcgtcccga aaaccttggc
      601 agccaagttc gcggtgcccg tgacattgaa catggcctat ccggtctacg gagtgcagtt
      661 ggttctcaaa ccgatcttgg cactcatggt gccgtttatt catcgactga caggcggaaa
      721 gggactaacc ctgccgctcg tgacggaaga agagctgaag attatgctcg acgagggcgg
      781 caaaacaggc gccatcgagg tcgaaaaggt caagatgatc aaaaatgtct ttcaattgaa
      841 ggacattact gctgaagatg cgatgacgcc tcgactctat gtgttttccc tggacgggaa
      901 ccttcgtctg aaggaagctc aagagctcct gtataactcg aagtactccc gcattcccgt
      961 ttacgacgga acgctcgaca acatcaccgg cattctctac aaaagcaggg cgttgaccga
     1021 gctggccaag aatcaaacgg acgccaagct gaaggagatt gcctatccgc cgatctttgt
     1081 gccggccggg aagacagcgg acgatctcat gaaacaattc cagcaggaaa agcggcacat
     1141 ggccgtggtg gtcaacgaat tcggcggcgt catgggcatt gtgaccctgg aagatttgct
     1201 cgaggaagtc gtcggcgaaa tcatggacga aacggacatc accgaagagc taatcaagcg
     1261 tttagggaag aaccacatcc ttgtgcacgg tcggaccgag gttcggaagg tcaacgactt
     1321 cctgaaagtc gacctcggcg acgaggccgt cacgatcg
//
LOCUS       DASTWE010000615         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1341878, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000615 DASTWE010000000
VERSION     DASTWE010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341878"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VFT30_06415"
     CDS             complement(<1..1109)
                     /locus_tag="VFT30_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU5092299.1"
                     /translation="MKRLSIATVFVGIIILIGFVATWARAAGPLASHATSPVVSICKS
                     PHKKRPVPAKTLQAIVRSHERWVEYRGNPDAKRMELCQADLSGATLSGANLERADLEG
                     TILRQAKLSQAILAQASLAGADLTKSVLEDSNLAGADLRHARLAGANLSRAVGDEAAL
                     FEAILIGAKLKGSSFERVQLEGADLTSADLTNGNFVEAHFYGATLKDTILVNTDLTGA
                     DLRRTILTNANLYRANLQGTLLDRAHLEGARLIEADLESAYLDETNLRNANLKKAILR
                     GADLRYADLTSADLHDADLEGANLEEATLINADLHSASLRMTILYYAVLDEAEFLDAN
                     LSHAVLIGAKGLRVNFMNADLSEMYAPKSSLTHAQ"
     gene            complement(1128..>2225)
                     /locus_tag="VFT30_06420"
     CDS             complement(1128..>2225)
                     /locus_tag="VFT30_06420"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5092300.1"
                     /translation="RLLDYLETLRFTGDVEAMLEGTTFFPHEPILRIIAPLPQAQLVE
                     SRVMSLLNFQTMIASKAARSVLIAAGKSLIDFGLRRAHGAEAGLLAARASYLAGFAGT
                     ATVLAGMAYGIPLYGTMAHSFVQTHEDELVAFEHFAQSQPENLVLLIDTYDTEAAAGK
                     VVSLAKTLKTKGITLKGVRLDSGDLADHARKVRRILDEGGLRCAQILASGNLDEYRLR
                     NLVQIGAPIDSFAIGTAMTTSSDAPSLDCAYKLQEYGGRPCRKRSEGKATWPGRKQIY
                     RYHMDDGRFDHDIVTTHDDRLPGEPLLQLVMKNGCLLAPSPDFAELRRYAAAQLEQLP
                     ESLQALETVPAYDVRISLALQSLAQTVDNRL"
BASE COUNT          480 a          592 c          653 g          500 t
ORIGIN      
        1 aattgtgcgt gagtgagaga agattttgga gcgtacattt cgctcagatc agcgttcatg
       61 aagttgacgc gcaatccctt ggctccgatg aggacagcgt ggctgagatt agcgtccagg
      121 aattcggctt cgtcgaggac ggcgtaatag agaatagtca tccgcagact cgcggagtgc
      181 aaatcggcat taatcagagt ggcctcttcc aggttggctc cttccaggtc ggcgtcatgc
      241 agatctgcgc tggttaggtc ggcataccgg agatcagcgc cgcgaagaat cgctttctta
      301 agatttgcat tgcgcagatt cgtttcgtcc agataggcgc tttccaagtc ggcttcgatc
      361 agacgagccc cttccaggtg cgcgcggtcc agtagtgtgc cctgaagatt agcgcgatag
      421 agattcgcgt tggtcaggat agtgcgacgc aggtcagcgc ccgtgagatc agtattcact
      481 aggatcgtat ccttcagggt tgctccgtaa aaatgtgcct cgacgaagtt gccgttggtc
      541 aagtcggcag aggtgagatc agcgccttcc aactgtacac gctcgaacga ggatcccttc
      601 aacttggcac cgatcaggat ggcttcaaag agagcagcct catcgccgac cgcgcgagaa
      661 agattcgcgc cagcgagccg ggcatggcgc agatcggcac cagcgagatt actgtcttcc
      721 aggacggatt tggtcaggtc agctcctgcg aggctggcct gagccagaat agcctgggat
      781 agcttcgcct gccgcaggat ggtaccttca agatcggccc gttcaagatt cgctcctgag
      841 agtgtagccc cgctcagatc agcttggcag agctccatac gcttggcatc cgggttacct
      901 cgatactcca cccatcgttc atgtgaacgc acaatggcct gcaatgtttt ggccgggacg
      961 ggccttttct tatggggact tttgcagata gaaaccacgg gcgacgtcgc atgggaagct
     1021 aacggaccag ccgctctggc ccatgtagcg acaaagccga tgaggatgat gattcccaca
     1081 aagacggtcg ctatggagag gcgcttcatc tggtatctcc ttaaaagtta gagcctattg
     1141 tcgacggtct gggctaagct ttgcaaagcc aaagaaatcc gaacgtcata ggccggtacc
     1201 gtctcaaggg cttggagcga ttcgggaagt tgctccaact gcgcagccgc atagcgccgc
     1261 agttcggcaa agtccggcga tggagcaagg aggcaaccat tcttcatgac aagttgaagc
     1321 agtggctccc caggcaatcg atcgtcgtgc gttgtgacga tgtcgtggtc aaaacgtcca
     1381 tcgtccatat gataacgata tatctgcttg cgaccgggcc acgtcgcttt gccttccgat
     1441 cgcttgcgac agggacggcc cccatattct tgaagtttat aggcacagtc cagcgatggc
     1501 gcgtcagagg aggtcgtcat ggctgtaccg atagcgaagc tgtcgattgg tgcaccgatt
     1561 tggactaagt ttctcaggcg atattcatcg aggttgccgc tggcgagaat ctgtgcgcaa
     1621 cgcaagcctc cttcatcgag aatgcgtcga acctttcgag catggtcggc caggtctccg
     1681 ctgtctaacc gtaccccttt gagggtgatg cccttggtct tcagtgtttt ggccaacgac
     1741 acgacttttc ctgcggcagc ttccgtatcg taggtgtcga ttaggaggac gaggttttcc
     1801 ggttgagatt gggcaaagtg ctcgaaggca acgagttcat cctcgtgggt ctgcacgaac
     1861 gaatgggcca tcgtgccgta cagcggaatc ccatacgcca tgccagcgag gacggtcgca
     1921 gttccggcga agcccgctag atagcttgcc cgtgcggcga ggagacctgc ttcagcgcca
     1981 tgggcacggc gcagtccaaa atcgatgagc gatttccctg ctgcgatcag caccgaccgt
     2041 gccgccttcg atgctatcat ggtctgaaag ttcaagaggc tcatcacacg gctctcaacg
     2101 agctgggctt gcggaagcgg tgcgataatt ctgaggattg gctcgtgggg aaagaacgtc
     2161 gttccttcga gcattgcctc cacatctccg gtgaaccgta gcgtttccag gtaatcaaga
     2221 agccg
//
LOCUS       DASTWE010000616         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1051116, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000616 DASTWE010000000
VERSION     DASTWE010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051116"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFT30_06425"
     CDS             <1..320
                     /locus_tag="VFT30_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092301.1"
                     /translation="NELAEIAMSQAQQARDLMDAALDFEAQGGSGQVLNLQPTDAAAI
                     AFNDYADTYSAAVAAAAAGPSVEFTQNNYSPESLSSAEIYRQSNNLLTFAAEKVTPNP
                     SAA"
     gene            338..>1113
                     /locus_tag="VFT30_06430"
     CDS             338..>1113
                     /locus_tag="VFT30_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092302.1"
                     /translation="MLQQVVIEAATPKTFQIDAVDPDDTLIVTSISGLDPADVTLFTG
                     EFARAGGYYQGRRTGQRNVVINLKINPDYASDIEVSDLREELYSLFFEPFATSDGVPM
                     RFVDDRKPDRRLVGYAEKMPSDLFSRDTKAQVSLICVDPFLLSVNPVVGNDAVGWATL
                     PLTYDGTARRGLNMTILVKTATASVNVDINGVKMTLTKTTGNFAVNDVITISTVDGFR
                     FIRQNGTDIMALLTASSKWLQLAKGVNTVKVYGTAEADGKA"
BASE COUNT          238 a          324 c          313 g          238 t
ORIGIN      
        1 cgaatgagct ggctgagatc gcaatgtctc aggcacagca agctcgcgac ctgatggatg
       61 ctgcgctgga cttcgaggca caaggcggtt ccggtcaggt actcaacctg cagcccacag
      121 atgcggctgc gatcgcgttc aacgactatg cagacacgta cagtgctgct gtggctgcgg
      181 ctgcggcagg accgagtgtg gagttcaccc agaacaacta ctcgcctgag tccctcagct
      241 ctgctgagat ctacaggcag tcgaacaacc tgctcacgtt cgctgcagag aaggtcacac
      301 ccaatccatc cgctgcctag taaggaaggg gcccgagatg ctccagcaag tggtcattga
      361 ggccgctacg ccgaagacgt tccagatcga tgctgtcgat ccggacgata cactcatcgt
      421 cacgagcatc tcgggcctcg acccagcgga tgtcactctc ttcaccggcg agtttgcccg
      481 agccggcggc tactaccagg gtcgccggac ggggcaacgt aatgtcgtca tcaaccttaa
      541 gatcaacccg gactatgcgt ccgacatcga ggtgagtgac ctccgtgagg agctttactc
      601 cctcttcttc gagccgttcg ctacctcaga tggtgttccc atgaggttcg tggacgaccg
      661 gaagcccgat cgtcggctcg tggggtatgc cgaaaagatg ccttccgact tgttctcgag
      721 agacaccaag gcccaggtgt ccctgatctg cgtcgatcca ttccttctgt cggtcaatcc
      781 ggtcgtcggc aacgatgcgg tcggctgggc aaccctccct ttgacctatg atggtactgc
      841 tcggcgtggt ttgaacatga ccatcctagt caagaccgcc acggcgagtg tcaatgtcga
      901 catcaacggg gtcaagatga ctttgaccaa gaccaccggc aattttgctg tcaacgatgt
      961 gatcaccatc agcaccgttg atggtttccg tttcattcgt caaaatggga ccgacatcat
     1021 ggctttgttg accgcttcgt cgaagtggtt gcagctagcc aagggtgtaa acaccgtaaa
     1081 ggtctacgga actgctgagg ccgatggaaa ggc
//
LOCUS       DASTWE010000617         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_436960, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000617 DASTWE010000000
VERSION     DASTWE010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436960"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VFT30_06435"
     CDS             <1..1061
                     /locus_tag="VFT30_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5092303.1"
                     /translation="HGGYMVGTYTTTKYVFDLKDDDVYFCVADPGWVTGHSYIVYGPL
                     LNGATILTAEGKPDYPNPGRWWNLIERYGVSIFYTTPTAIRLLMRYGEDWPKKFDLST
                     LRILGSVGEPINPEAWEWFHRVTGEDKPIMDTWWQTETGSILITPLPTVPLKPGSATR
                     PFLGIEADVVDREGNSLPVNAGGFAVIKKPWPSMMRAIYKDPERYKAYWFTIPNCYTA
                     GDVCHKDADGYLWFMGRADDVIKVAGNRLGTAEVESALVSHPAVAEAAVIGKPHKTVG
                     ESIKAFIILKQGEQESPELIKSIKDQVLKELGKIGVPSEIDIVSSLPKTRSGKIMRRV
                     LKAKELGQDPGDISTIEE"
     gene            1117..1566
                     /locus_tag="VFT30_06440"
     CDS             1117..1566
                     /locus_tag="VFT30_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU5092304.1"
                     /translation="MLKLNARAAIVFIGFMSLSPMLGMELTLAEDQKPKATPKAKAIR
                     PKAEPKFHTAETVTRATACFGEAPQIDKLVPDEGRPGTHVTIKGTQLGSPDCLRGVSF
                     GPGYASTFTMKNESTIVTTVPTGGRKGLVMLTVTTASGEDSKVFMVK"
BASE COUNT          428 a          467 c          439 g          342 t
ORIGIN      
        1 tccatggagg gtacatggtc ggcacctata cgacgacgaa gtacgtgttc gacctaaaag
       61 atgatgacgt atacttctgc gtggctgatc cagggtgggt cacaggccac agctatatcg
      121 tctatggtcc tctcctcaat ggagcaacga ttctgaccgc cgaagggaaa ccggattatc
      181 cgaaccctgg acgctggtgg aatctcatcg aacggtatgg ggtatcgatt ttctatacga
      241 ctccgaccgc tatccgactg ctcatgcgct acggcgaaga ctggccgaag aaattcgatc
      301 tctcgacgct ccgtatcctc gggagcgtcg gagaaccaat taacccggaa gcctgggaat
      361 ggtttcatcg tgtgacaggc gaagacaaac ccatcatgga cacctggtgg cagacggaaa
      421 cgggatcgat cttgatcacc ccgcttccca ccgtcccgtt gaaacccggc tcggctaccc
      481 gaccatttct cggcattgaa gccgatgtcg tcgatcgcga gggaaacagt cttcctgtaa
      541 acgccggcgg ctttgccgtc attaaaaaac catggccatc catgatgcgc gccatttaca
      601 aggatcctga gcggtataag gcctactggt tcaccatccc caattgctat acggccggag
      661 acgtctgcca taaggatgcg gatggctacc tctggttcat gggccgcgcg gatgacgtga
      721 tcaaagtcgc cggcaatcgg ctcggcaccg ctgaagtgga aagcgcattg gtcagtcacc
      781 ctgccgtcgc ggaggctgcc gtgatcggca aaccgcacaa gacggtcggt gaatccatta
      841 aggccttcat catcttgaaa cagggagagc aggaaagtcc ggagctgatc aagtcgatca
      901 aggatcaggt ccttaaagaa ttgggcaaga tcggcgtccc gtctgaaatc gatatcgtct
      961 cttccctccc caagactcgg tcaggaaaaa ttatgcgccg cgtactcaaa gcgaaggagc
     1021 tgggacaaga cccaggggac atctcgacca tcgaagaata acggtcacac gaactccgtg
     1081 cggtatggat caattcattg atgaagggaa taacccatgc tgaaactcaa cgctcgcgct
     1141 gccatagttt ttatcggttt catgtctttg tccccgatgc tcggcatgga actcacctta
     1201 gccgaggacc agaaaccaaa agctacgccg aaagccaagg ccattagacc gaaagcggaa
     1261 ccaaagtttc acacagctga aacggtcacg agggcgacag cctgtttcgg ggaagctccc
     1321 cagatcgaca aactcgtgcc ggacgaaggc cgaccaggca cgcacgtcac gatcaaaggg
     1381 acccaattgg gctcgcctga ctgcttgcga ggcgtttcct tcggcccagg ctacgcttcc
     1441 acctttacaa tgaaaaacga atccaccatc gtaacgacag ttccaaccgg tggacgtaag
     1501 ggcctcgtca tgctaaccgt cacaacggct tccggagaag attccaaagt gttcatggtt
     1561 aaataggaga atgccggttt tttagtatga aatcgacgag gaaaccaggc gagcctatct
     1621 atctgagacg acacataata agccttgccc tagccgtaat ccttccccta gtcctg
//
LOCUS       DASTWE010000618         1308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_437004, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000618 DASTWE010000000
VERSION     DASTWE010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437004"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..637
                     /locus_tag="VFT30_06445"
     CDS             65..637
                     /locus_tag="VFT30_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092305.1"
                     /translation="MMNNALRLGEIVVAGFFLWSQAAPAEDLPLTENVPISEFQNRIE
                     DAKTYDFDAPPQGMFRSIVLAEDFEERLGFQRTLEIAPIKPTERFPADVAAIFIVFSL
                     HQHYQGFKVFGRCTPEKVAGVAPQPIVSEDAMHIALEDDSGYLKLSAPQGGWMPGRYK
                     VEIHVGEQINETSLMGTMRFTIVTSRKQPD"
     gene            749..>1308
                     /locus_tag="VFT30_06450"
     CDS             749..>1308
                     /locus_tag="VFT30_06450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5092306.1"
                     /translation="MTPPSSPWASVIIPIKDERENLSPLTASLLKVMDSHELSRSRPY
                     EILLVDDGSSDGSSDELDRLAREHSQVRVFHFDRNYGKTCALEAGFRQSSGEIIIQID
                     GDLQQDSEDILKLLPYTASHDVVCGWRQQRQDGLVKKFSSLIANRIRNIFTHDGMHDT
                     GCPLKVFRRPVLERIRLFEGMHRFFPA"
BASE COUNT          292 a          376 c          341 g          299 t
ORIGIN      
        1 cgatagtttg cgatggtttg atgatttatt gacagtgtcg cctcaagagg tgtagcagac
       61 agtgatgatg aacaacgctc tccgtctagg cgaaatcgtg gtagccggat tcttcctgtg
      121 gagccaagcg gctccagccg aagatctgcc tctcaccgaa aacgtaccga tctcagagtt
      181 tcaaaaccgc atagaggacg caaaaaccta cgatttcgat gcgcccccgc agggtatgtt
      241 tcgttccatt gttttggcgg aagactttga ggagcgactt ggatttcaac gcactctcga
      301 aatcgcgccg attaaaccga ctgaacgatt tcccgcggat gtggccgcca ttttcatcgt
      361 gttctctctc catcagcact atcaggggtt caaggtcttc ggccgctgta cacccgagaa
      421 agtagccggc gtcgcgccgc agccgatcgt cagcgaagac gccatgcaca tcgccttgga
      481 ggatgacagc ggctatctca agttgtcagc cccgcaggga ggctggatgc ccggccgata
      541 caaggtcgaa attcatgtgg gcgagcagat caatgagacg agtttaatgg gcacgatgcg
      601 tttcaccatt gtcacgtctc gcaaacagcc cgattgacga tcgaccccag gtccatctcc
      661 ggcacgcgtt tgaccctctc tccaccttcc gttagaatgc gccatttcat atcgtgacct
      721 ccggccactg ctcgagtttt tgcatatcat gacgccacca tccagccctt gggcctctgt
      781 catcatcccg atcaaggatg aacgagagaa cctgtctcct ctcacagcaa gcctgctgaa
      841 agtcatggat tcgcacgaac tgtcacgctc ccgcccttac gagattctcc tcgttgacga
      901 tgggagcagc gacggcagca gcgacgagct ggaccgcctg gctcgcgaac attctcaagt
      961 gcgcgtgttt cacttcgacc gaaattacgg caagacgtgc gcgcttgagg ctggttttcg
     1021 gcaatcttcg ggcgagatca ttatccagat tgacggtgat ctccagcagg acagcgaaga
     1081 tatcttgaag ctgcttcctt ataccgcctc ccacgacgta gtctgcggat ggaggcagca
     1141 acgacaagac ggcttggtga agaaattctc ctcattgatt gccaaccgca tacggaatat
     1201 cttcacccat gacggcatgc acgacacggg ttgcccactc aaggtcttcc gacggccggt
     1261 actggagcgg attcgtctct ttgagggaat gcaccgattc tttccggc
//
LOCUS       DASTWE010000619         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_695723, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000619 DASTWE010000000
VERSION     DASTWE010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695723"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFT30_06455"
     CDS             <1..822
                     /locus_tag="VFT30_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5092307.1"
                     /translation="SMYEDLTVLENIRFFAEVRGLRSDEWLPRCMEILEFVGLDTFTD
                     RRAGQLSGGMKQKLGLASALVTRPRVLLLDEPTTGVDPVTRQDFWQLIIKLASSSPSG
                     IEIEGAHETRPSTSLRSAQGEVSVIISTPYMDEASRCHRVGFMKNGKIIAEDTPSNLR
                     ARLNNRVLELRGSPIGLLRHVAHKDQDVEDVQAFGDRLHIRVGEHKAQAVLGRLTSEI
                     ENEGGHVDELRSVPPVLEDVFIALSESESMSPDIALNQRRVPMGDDKTLTPGEHP"
     gene            819..>1105
                     /locus_tag="VFT30_06460"
     CDS             819..>1105
                     /locus_tag="VFT30_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5092308.1"
                     /translation="MSEAVIQVENLTRRFGDFVAVDHISFDVRAGEVVGYLGPNGSGK
                     TTTIRMLLGLIAPSDGKATVLGFDAFRQSEEVRARAGYMSQKFAIYDDLTVL"
BASE COUNT          238 a          289 c          331 g          247 t
ORIGIN      
        1 tcgatgtatg aagatctcac cgtgcttgag aatattcgtt tctttgccga agtgcgcggg
       61 ctgaggtcgg atgagtggct gccacgctgt atggaaatcc ttgaatttgt tggactggac
      121 acatttaccg accgccgggc cggccaactc tctggtggga tgaaacaaaa acttggcctt
      181 gcttctgcac tggtgacgcg tccgcgcgta ttgctgctgg atgaacccac cacaggggtt
      241 gaccccgtga cgcgccagga tttctggcag ttaatcataa agcttgcatc ttcctcgccg
      301 agcggcatag aaatagaagg tgctcatgaa acacgccctt cgacttcgct ccgctccgct
      361 cagggggaag tgtccgtcat catttccacg ccttatatgg acgaagcctc acgttgtcat
      421 cgtgtaggat ttatgaagaa tggaaagatt attgccgaag ataccccgtc caacctgcgg
      481 gcgcggctca acaaccgtgt cctggagctg cgcggctcgc cgatcggtct tctccgtcac
      541 gtggcgcata aggatcagga tgtggaagac gtgcaggctt tcggcgaccg gctgcacatc
      601 cgcgtggggg agcacaaggc tcaggctgtc ctcggcaggc tgacgtctga gatcgaaaac
      661 gaaggcgggc acgtggatga gcttcgttcc gtgcctcctg tcctcgaaga tgtatttatc
      721 gcgctgtcgg aatcggagtc gatgtctcca gatatcgcac tcaaccagcg tcgggtcccc
      781 atgggggatg ataagacctt gactccagga gagcatccat gagcgaagca gtcatccaag
      841 tggaaaacct tacgcggcgc ttcggggatt tcgtcgcggt cgaccacatc agcttcgatg
      901 tgcgcgccgg cgaggtcgtt ggatatctcg gtccgaacgg gtcaggcaag acaacgacga
      961 tacggatgtt gctgggcttg atcgcaccga gcgatggtaa agcaacggta ctgggattcg
     1021 acgcgttcag acagagtgaa gaagttcgtg cgcgtgccgg gtacatgtca caaaagtttg
     1081 cgatctatga cgatctgaca gtgct
//
LOCUS       DASTWE010000620         1471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_986669, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000620 DASTWE010000000
VERSION     DASTWE010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986669"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="truD"
                     /locus_tag="VFT30_06465"
     CDS             complement(<1..679)
                     /gene="truD"
                     /locus_tag="VFT30_06465"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEU5092309.1"
                     /translation="MTQPIAPYLTGDLPGIGGQIRTVPEDFQVEERPLYLPCGEGEHL
                     YMAITKRGLSTPDLVRRLSSTLGVKAQAIGVAGLKDARAVTTQMVSLQGIHPEQISRL
                     RIDDTILNVQILGRHRNRLRTGHHSGNRFRLVIRDVADHAAETVPAVLQQLSLRGIPN
                     YFGPQRQGKDGDNYRIGAMLLHDTRRREKMSRATRIWYLNSYQSFLFNRILARRIDHL
                     DKLFVGDW"
     gene            804..>1471
                     /locus_tag="VFT30_06470"
     CDS             804..>1471
                     /locus_tag="VFT30_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5092310.1"
                     /translation="MSRRRTVSWPDRVRSFIGYYLSEPLYRAFSVVPHWEWGLSDHDV
                     SILTHPHMFLVGRRAVHLTDLHLDRYHPRHDRVVESVRELHPDWIFITGDLLNVPEGL
                     PHLFRFLEQLRAIAPLYMTLGNHDHYSGVSVSQFSELADRHKITLLVNQCVIVSTGNG
                     ELAIIGVDDPSLHRADLRCVPPRADARFTLLLAHAPNILDYVEPHHAIDLILCGHSHG
                     GQWRV"
BASE COUNT          304 a          389 c          423 g          355 t
ORIGIN      
        1 cccaatcgcc gacgaagagc ttgtcgagat ggtcgattct tcgtgcaagt atccgattga
       61 acaagaatga ttggtaggag ttgagatacc agatgcgggt agcacgactc atcttctcgc
      121 gccgccgcgt atcatgcaac aacatggcgc cgatgcggta gttatcaccg tctttccctt
      181 gccgctgtgg cccgaagtag tttggaatgc cgcggaggct cagttgttga aggacggccg
      241 gcacggtttc agccgcgtga tcggcgacat cgcggatgac taaacggaag cgattgccgg
      301 agtgatggcc ggttcgtagt cggttacgat ggcgcccgag gatctgcaca tttaggatgg
      361 tgtcatcgat cctgaggcga gagatctgtt ccggatggat tccttgcaag gacaccatct
      421 gagtcgtgac ggctcgcgcg tccttcaggc ccgccacgcc gatggcttgt gctttaacgc
      481 ccagcgtgga agagaggcga cggaccagat ccggagtgga aagaccacgt ttggtgatgg
      541 ccatgtacaa atgttcgcct tccccgcaag gaagatagag cggccgttct tcaacttgaa
      601 agtcttcggg cacggtgcgg atttgtccac cgattccagg cagatcgccg gtgaggtagg
      661 gggcgattgg ttgcgtcacg cattgtcctt cattggaatg acggctgtcc gcggaaatgt
      721 gttgtcaaat caagtgggaa acaagcccat ggtatacttt tttcgtccat gcggaaacga
      781 tcacgcaaac gacagggtgc ggaatgagca ggcgacggac ggtgtcatgg cccgatcgcg
      841 tgcgatcgtt tatcgggtat tacttaagtg agccgctcta tcgggcgttc agcgtggttc
      901 ctcattggga atggggtttg tccgatcatg acgtttccat cctcactcac ccacatatgt
      961 tccttgtcgg tcgtcgcgca gtccatctca cggacctgca cctggaccga tatcatccga
     1021 ggcatgatcg tgtcgtcgaa tccgtgaggg aacttcaccc cgattggatt ttcatcaccg
     1081 gggacctgct caacgtaccg gaaggtctgc cccatctctt tcgatttctc gaacagctac
     1141 gcgccatcgc gcccctgtac atgacattgg gcaatcacga ccattatagc ggagtgtctg
     1201 tgtcacagtt ttctgaactt gccgatcgac acaagatcac gctgctggtg aaccaatgtg
     1261 tcatcgtttc aacgggaaat ggagaattag caatcatcgg tgtcgatgac ccatcgcttc
     1321 atcgtgcgga tcttcgatgt gtcccgcctc gcgccgatgc tcgttttaca ttgctgctgg
     1381 cacatgctcc caacattctc gactatgtcg agccgcatca tgccattgac ttgattcttt
     1441 gcggacacag tcacggcggg cagtggaggg t
//
LOCUS       DASTWE010000621         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_760663, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000621 DASTWE010000000
VERSION     DASTWE010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760663"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VFT30_06475"
     CDS             <1..1543
                     /locus_tag="VFT30_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5092311.1"
                     /translation="CLATSGPGGIHLLNGLYDAKFDGQPVLALTGHTFHDLIGTHYQQ
                     DIDLDRVFSDVASFSERVMGPTHVCNVVDQAVKTALSKRTVSHITIPKDIQDWPADGM
                     RSKANVAEHSGDRFNEALPLPGEAQLERAAALLNEGTKVAILAGRGCLSARDEIVMLA
                     EYLGAPIVKPLLGKAVVPDDHPYTTGGIGLLGTAPSQDVMEECDTLIIAGSSFPYLEF
                     YPKPGQAKAVQIDLDPARIGLRYPVNVGLVGQCRDVLQVLLPLVAPKSDRRFIEWAQE
                     RKARWDTLMEERGMRDDIPMKPQVIAHVLNDVLADDAIICCDTGTVTTWAARHIRVRA
                     TMQFSVSGTLATMGNGLPYSIGASVAFPGRQVVCFAGDGGFTMLMGELATAVKYQLPI
                     KIIVVKNNSLGMIKWEQIAFEGNPQYGVDLHAIDFASFARACGAIGYSVTDPARVRET
                     LQEAFAQPGPVLIEAVVDPYEPPLPGKITTDQAWQFTKAMARGQKDRLEIVKTVIENK
                     IREVV"
     gene            1821..2429
                     /locus_tag="VFT30_06480"
     CDS             1821..2429
                     /locus_tag="VFT30_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU5092312.1"
                     /translation="MDFVTNIEEIRKRARRHIEEGPVTEGYALDRKQAIRVLNDALAT
                     EYMCVLRYRFHYFMATGINSSAVAEEFMEHAREEQAHADQLAERIKQLGGKPELHPSV
                     IAERSHSEYREGTSLADMIREDLIAERIAIETYREMVRYFGDKDTTSRVMLEGILAKE
                     EEHADELADLLFAVQPDTNQGSRRLYFGDEVSGRAERDQVRS"
BASE COUNT          600 a          626 c          741 g          532 t
ORIGIN      
        1 ctgcttggcc acgtctggtc ctggtgggat ccacctcttg aacggcttat acgatgccaa
       61 attcgacgga caaccggtat tggccctcac aggtcacacg ttccatgatc tgataggcac
      121 ccattatcag caggacattg atctcgaccg tgttttcagc gatgtggctt ccttcagcga
      181 acgcgtcatg ggtccgactc atgtgtgtaa tgtggtcgat caggccgtca agaccgctct
      241 ctcgaaacgc accgtttcac acatcacgat tcccaaagat atccaagatt ggccggcgga
      301 tggaatgcga tccaaggcga atgtcgccga acacagtggc gataggttca acgaagcgtt
      361 gccgctcccg ggagaggcac aactggaacg agcagccgca ttactgaatg aaggaaccaa
      421 agtggccatt ttagctgggc gaggctgttt gtcggcgcgt gatgaaattg tcatgttggc
      481 tgaatacctc ggagcaccga tcgtgaagcc gctgctcggg aaagcggttg ttccggacga
      541 ccatccctac acgaccggag gaatcggcct gttgggcacg gctccgtcgc aggacgtgat
      601 ggaggaatgc gataccttga tcatcgcagg cagcagcttt ccctacctcg agttttaccc
      661 caaaccggga caggccaaag ccgtccaaat cgatctggac ccggccagaa tcggattgcg
      721 gtatccggtc aatgtcgggc tggtcggaca atgccgggac gtgcttcaag tactgttgcc
      781 tttggtcgct ccgaaatcag accggcgctt catcgagtgg gcacaagagc ggaaagcccg
      841 ttgggatact ctcatggaag aacgaggcat gcgtgatgac attcccatga aaccacaagt
      901 tatcgcccat gtgctcaacg acgtactcgc tgacgatgcg atcatttgtt gtgataccgg
      961 aacggtgacg acatgggcgg cgcgtcacat ccgcgtgcgc gccacgatgc agttctctgt
     1021 atcgggaacc ttggctacga tgggcaatgg actgccgtac agtatcgggg cgtcggtcgc
     1081 cttccccggt cgacaagtgg tctgttttgc gggggacggc ggttttacca tgttgatggg
     1141 cgaactggcg actgcagtca aatatcagtt gccgatcaag atcattgtcg tcaagaacaa
     1201 ttcccttggc atgatcaagt gggagcagat tgcgttcgag ggaaaccccc aatatggcgt
     1261 ggatctgcac gccatcgatt ttgcatcatt tgcccgcgca tgcggggcga tcggatattc
     1321 cgtgactgac ccggcccgtg tgagggagac tctacaggaa gcttttgcac agccgggacc
     1381 tgtcctgatc gaagcagtgg tcgatccgta tgaaccacca ttgccgggga agatcacgac
     1441 ggaccaggca tggcagttca ccaaagccat ggctcgagga caaaaagatc gtttggaaat
     1501 tgttaagacg gtcattgaga acaagatacg cgaagtagtt tgaagtggtt taacgtgcgt
     1561 ttatcgcgat ggttactcca gcacgtcccg ccggtttttc agtacaaatt gtctttcgat
     1621 agacagattg cccccaacag tgttgcttgg tttgctcatg agcatgtcgg agaaagctat
     1681 gctgatgacg tgcatcatct tttcaagggt atggcgcggg atttgtcgtg gtcactgaga
     1741 aaggacaagg caaagtttgt gcaatcaact gctgagccag gaagaaggcc gaggcgccgt
     1801 taggaaggag agggaagcac atggatttcg tgaccaacat cgaagagatt cgaaagcggg
     1861 cccgccggca tattgaagaa ggcccagtca cggagggata tgcgctggac cggaagcagg
     1921 ctatccgagt gctgaacgat gcgttggcca ccgagtatat gtgcgtgctt cgttatcgat
     1981 tccactattt catggcgaca ggcatcaact catcggcggt tgccgaagaa tttatggagc
     2041 atgctcggga ggaacaggcc catgccgatc agctcgcgga gcgaattaag cagttgggag
     2101 gcaagcccga actccatccc tcggtcattg cggaacgtag ccacagcgaa tatcgggaag
     2161 gtacttcttt agccgatatg attcgcgaag atctgatcgc cgagcggatt gccatcgaga
     2221 cctataggga gatggtgcgg tacttcggcg acaaggatac gacctcccga gtcatgctgg
     2281 aaggcatctt ggcgaaggaa gaagagcatg cggacgaact ggcggacttg ctgtttgccg
     2341 tgcaaccgga cacaaaccag ggctcgcgac gtctatactt cggtgatgag gtttcgggtc
     2401 gtgcggaaag agaccaagtc aggtcgtaat aagagcaaca tcggtcggca gtccaggcat
     2461 aaaacacacg aagggagacc cagatggcaa agaaacaac
//
LOCUS       DASTWE010000622         1481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_178587, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000622 DASTWE010000000
VERSION     DASTWE010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178587"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VFT30_06485"
     CDS             <1..1164
                     /locus_tag="VFT30_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5092313.1"
                     /translation="DSIGKGVDTSGIVLELRRKDDGKPLWIKWWSRPDPGGTYTRTMF
                     VDITEQVLMEQEKARLEAQNVYLQEEIKGSHNFEELIGSSTSLKKILKNVERVAPTDS
                     TVLITGETGTGKELIARAIHNLSPRKDKPLVKVNCAAIPFGLIESELFGHEKGAFTGA
                     LTKKMGRFEVADKGTIFLDEIGELPLDLQPKLLRVLQEGEIERVGGTQTFKVNVRVIA
                     ASNRNLEQLSKSGQYRLDLFYRLNVFPVHLPALREREGDIPLLAQYFAQKHAANFGKK
                     INRLSEGMMTALQRYPWPGNIRELEHVIERAVILSEGPELEPIDWLSPPSGKVFGNGK
                     ALTLEEIERQHIRDVLEQTNWRVSGDKGAAAILGLKPTTLEARMKKLGISRPG"
     gene            1161..>1481
                     /locus_tag="VFT30_06490"
     CDS             1161..>1481
                     /locus_tag="VFT30_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092314.1"
                     /translation="MIELRFLDDTIQDFMPVSFSIPLTSCATLIAIALMIFSTTTVVH
                     AQVQCWVPRQNEGLLSDKRWARHLKAMSAAEAIVKQSEEFLNPPVPVRMRTTMAAHRY
                     EPMDS"
BASE COUNT          357 a          382 c          437 g          305 t
ORIGIN      
        1 gactctatcg gcaagggtgt cgacacaagc gggatcgtgc tggagctgcg tcggaaagac
       61 gacggcaagc cgctgtggat caagtggtgg tcacggcctg acccaggcgg tacctacacc
      121 cgtacgatgt ttgtggacat caccgaacag gtgttgatgg agcaggagaa ggccaggctg
      181 gaagcgcaga atgtctacct ccaagaggag atcaaaggca gccataattt tgaggaactg
      241 atcggttcat ccacctccct caaaaagatc ttgaagaacg ttgagcgtgt ggcgccgact
      301 gattcaacag tgctgattac gggcgagacc ggtaccggca aagagctgat cgcgcgagcc
      361 attcataact tgagtcctcg gaaggacaag ccgctggtca aggtgaactg cgccgccatc
      421 cccttcggtt taatcgagag tgaactgttc ggtcatgaga aaggcgcctt cacgggcgcg
      481 ttgacgaaaa agatggggcg cttcgaagtg gccgataaag ggacgatctt cttggatgag
      541 atcggagaac ttccgctcga tctgcaacct aaactgttgc gtgtcttgca ggagggtgaa
      601 atcgaacgag tcggggggac tcagacgttt aaggtgaacg tgcgcgtgat cgccgcctcg
      661 aaccgcaatc ttgagcagct ctcgaaatcc gggcaatacc ggctcgacct cttctatcgt
      721 ttgaatgtct tcccggttca ccttcctgcc ttgcgcgagc gggaaggtga cattccgtta
      781 ctggcgcagt attttgcgca gaaacacgcg gcgaatttcg ggaaaaagat caatcggctt
      841 tccgaaggaa tgatgacggc cttgcaacgt tatccgtggc cgggaaacat tcgcgaactg
      901 gagcacgtca ttgaacgggc ggtgattttg agcgaaggac cggaattgga gccgatcgat
      961 tggctgtcgc ccccaagcgg caaagtcttc ggaaacggga aagccctgac gctcgaagaa
     1021 atagaacgac agcacatccg tgatgtcttg gaacagacca actggcgcgt cagcggagac
     1081 aagggagctg cggcaatttt ggggctgaaa cccaccacac tggaagcccg tatgaagaaa
     1141 ctgggtatca gcaggcccgg ttgatcgaac tgcggtttct cgacgatacg attcaggact
     1201 tcatgccggt ttcattctct atccctctta catcgtgtgc cacactcatc gcgattgcac
     1261 tcatgatctt cagcacgaca accgttgttc acgcacaggt acagtgttgg gtgccgcgtc
     1321 agaacgaggg gcttctaagc gataaacgat gggcgcgtca cctcaaggcc atgagcgccg
     1381 cagaagctat tgtcaaacag tctgaagagt tcctgaatcc gccggtcccc gttcgcatgc
     1441 ggaccacgat ggctgctcac cgttatgagc cgatggactc g
//
LOCUS       DASTWE010000623         1276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_49332, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000623 DASTWE010000000
VERSION     DASTWE010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49332"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFT30_06495"
     CDS             <1..247
                     /locus_tag="VFT30_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092315.1"
                     /translation="AVIMVSLPNSAPALPPVQGEVVSMTAGIPGTMVIRDEKGQLHIL
                     NLTQQTQLGGKFKVGDKVLAFSGPYGVSAVQLQMGNR"
     gene            complement(318..506)
                     /locus_tag="VFT30_06500"
     CDS             complement(318..506)
                     /locus_tag="VFT30_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092316.1"
                     /translation="MARRISIRWKVLQALGAILIAMGLVVDWPPPQEAGLPDTSSFLI
                     ILGGLLGAAGFLAVLRRE"
     gene            696..1031
                     /locus_tag="VFT30_06505"
     CDS             696..1031
                     /locus_tag="VFT30_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092317.1"
                     /translation="MVISAYTGLTQTIQDLRRKGFTANFEFLDHVFTDVESGRTFTAD
                     HLAIIDHRRFEGMSDPDDMSVLYAIESNDGTKGIIVDAFGTYADPELGDFLQGVRYRE
                     RSRLQALAA"
     gene            complement(1063..>1276)
                     /locus_tag="VFT30_06510"
     CDS             complement(1063..>1276)
                     /locus_tag="VFT30_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092318.1"
                     /translation="DLLVAHQEPAQVTSTQNALVADVVLEGTASAMGQLPIQQRTQKS
                     QLRGTQAPQATRVPDEEIIEELPNAP"
BASE COUNT          281 a          321 c          365 g          309 t
ORIGIN      
        1 ggccgtgata atggtcagcc ttcccaattc tgctcctgct cttccgcccg ttcaaggaga
       61 agttgtctcc atgacggccg gtattcctgg aacgatggtc attcgagatg agaaagggca
      121 gctccatatt ctgaatctca cgcaacaaac tcaactcggt ggtaagttta aggtgggtga
      181 caaggtgctg gccttctccg gcccatacgg ggtgagcgca gttcagctcc agatgggcaa
      241 tcgttaacca gaggcttcat ttttcagact ccatagaatc gaaagaatcg aacattcgcc
      301 ttttcattga cgcgagtcta ctctcgccga agtacagcaa ggaatcctgc cgcaccgagt
      361 agcccgccta ggatgatcag aaacgatgac gtgtccggta ggccggcttc ctgcggaggc
      421 ggccaatcga cgaccaggcc catcgcaatg aggatggcgc cgagagcttg caagaccttc
      481 caccggatcg agatccgccg ggccatctcc gttgatcttt cttcagtctt tctctccatg
      541 ggtcccacat ccgggcgatt cagcataaca tggtgagaag cggcgatggt caagtggtat
      601 ccccgatatt actcaggacg tgaacgcgtg actgctctct ccacgatcgg gagtgttatg
      661 atgaccgtat ggagaaggta gaggaaggag gcaacatggt catatccgca tacactggat
      721 tgactcaaac cattcaggat ttacgaagga aaggattcac ggcgaatttt gagtttctgg
      781 atcatgtgtt cacggatgtc gagagcgggc ggacatttac cgccgatcat cttgctatca
      841 tagaccatcg gcggtttgag ggaatgagtg atcccgacga catgtcggtt ctctatgcaa
      901 tcgaaagtaa tgacggtacg aaaggaatca ttgtggatgc gtttggaacc tatgccgatc
      961 ctgaactcgg agactttctc cagggagttc gctatcgtga gcgttcacgg cttcaggcgc
     1021 ttgcggcctg aagccgtggg aaaccgtgat caggcttcgt gtctacgggg cgttcggcag
     1081 ttcttcaata atttcttcat ctgggacgcg cgtggcttgt ggcgcttgag tgcctcgcag
     1141 ctgggacttt tgtgttcgtt gctggatcgg caattgtccc atagcactgg ctgtgccttc
     1201 gagaacgacg tcggccacga gggcattctg ggttgacgtg acttgagccg gctcttgatg
     1261 agccacgagt agatcg
//
LOCUS       DASTWE010000624         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_825078, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000624 DASTWE010000000
VERSION     DASTWE010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825078"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFT30_06515"
     CDS             <1..294
                     /locus_tag="VFT30_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092319.1"
                     /translation="TRVVERGQAQRQAAAQGRGVTATSCQSALFMNASTATVYTPLRQ
                     AWPRFVCGQAGRLALRDLNILFQVCLGLLGLRMPVSHGVSAVSSRTAMNNGGY"
     gene            308..673
                     /locus_tag="VFT30_06520"
     CDS             308..673
                     /locus_tag="VFT30_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5092320.1"
                     /translation="MKHNPGFLQLVEQARRRVKECSVADVKARLDRGERFHFIDIRED
                     HEFAKDHARGARHLGKGIIERDIESVVPDKQDSIVLYCGGGYRSALAADVLQQMGYGN
                     VISMDGGIRAWRDAGYPLE"
     gene            complement(692..1240)
                     /locus_tag="VFT30_06525"
     CDS             complement(692..1240)
                     /locus_tag="VFT30_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU5092321.1"
                     /translation="MHIPALRTLLLTVLLSPLVLLMTISVQAAEFKMGVVDPQSVLEK
                     SKAGKRALEGLKEYVSTRQKLLAKDEEDLRNYEKQLKEQAPKWTDAEKKDKEGQFRTK
                     VQDFQKRAQEFNMELQKKQKELVDEYMKKISASTQAVAEKGGVSIVVDKGSEQTVKIV
                     IYNKDTIDLTDQVIKEFDRVNK"
     gene            complement(1324..>1641)
                     /locus_tag="VFT30_06530"
     CDS             complement(1324..>1641)
                     /locus_tag="VFT30_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092322.1"
                     /translation="SQRSQLKGRVIELFRTHRSSNGSEEVALVELNQTPRIFQLNMLA
                     LAMKDLGYPPPFKKEKLQKIKNPFDPSHADEYAMRAVARRLKWHYGVEVWIAEESISF
                     DSW"
BASE COUNT          338 a          430 c          452 g          421 t
ORIGIN      
        1 acccgtgtcg tcgaaagagg acaagcccaa cgacaagcgg ccgcacaagg tcggggtgta
       61 acggctacgt cctgtcaatc cgcattattc atgaacgctt ctactgcaac cgtctatacg
      121 ccgctgcgac aggcctggcc gcggtttgtc tgcggccagg cgggtaggct cgcccttcgc
      181 gacctcaaca tactgtttca agtatgcctc ggcctcttag ggctccgcat gcccgtctcg
      241 catggcgtct cggcagtttc gtcacgaacc gccatgaata atgggggcta ttagaggaga
      301 catgaacatg aaacataatc cagggttctt gcaacttgtc gaacaggcga gacgacgtgt
      361 caaggagtgt agcgtggccg atgtgaaggc tcggcttgac cggggcgagc ggttccattt
      421 catcgatatt cgggaagatc atgaattcgc caaggaccat gctcgtggcg ctcgacatct
      481 tgggaaaggg attatcgagc gggatatcga atcggtagtt ccggataagc aggactcaat
      541 cgtactctat tgtggcggag ggtatcgatc ggcgctggca gccgatgtct tgcaacagat
      601 ggggtatggg aatgtcatct cgatggacgg agggattcgt gcgtggcggg atgccggtta
      661 tccgttggag tagcgtgacg acggcttaaa attacttgtt cacccgatcg aactccttaa
      721 tgacctgatc ggtgagatca atcgtatctt tgttgtagat cacgattttg acggtctgct
      781 cgcttccttt gtcgacgacg attgacaccc ctcctttttc tgcgactgct tgtgtcgagg
      841 cggagatttt tttcatgtac tcgtcgacca actccttctg tttcttctga agttccatgt
      901 tgaattcctg cgcacgcttt tggaagtcct ggaccttcgt ccggaattgc ccctccttgt
      961 ccttcttttc cgcgtcggtc cacttggggg cctgctcctt caattgtttc tcataattgc
     1021 gaaggtcttc ctcatccttg gcaaggagct tctgcctggt cgagacatat tccttcagcc
     1081 cttcgagcgc tcgtttcccc gccttggact tttctaagac ggactgcggg tccaccaccc
     1141 ccatcttaaa ttccgcggct tggacggata tcgtcatgag caaaacaagc ggtgagagga
     1201 gcactgtaag gagcaaggtt cgcaaagcag gaatatgcat atcgtctctt gtgcctcctg
     1261 atctggatga gggaaccgcg gcagaataac cgtgcaccga aggtctgtca agcgatcaga
     1321 tgactaccac gaatcaaaac tgatcgattc ttccgcgatc cagacctcga cgccatagtg
     1381 ccatttgagg cgtcgcgcga ccgctcgcat cgcgtactca tccgcgtggc ttggatcgaa
     1441 ggggttcttg atcttctgga gcttttcctt tttgaacgga ggggggtagc caaggtcttt
     1501 catggcgagc gccaacatgt tgagctgaaa gatgcgcggc gtttggttca gctctactaa
     1561 tgcgacctcc tccgatccat ttgaagagcg gtgcgttcgg aataattcaa taacccggcc
     1621 tttcaactgg gagcgttgag a
//
LOCUS       DASTWE010000625         1303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_501907, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000625 DASTWE010000000
VERSION     DASTWE010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501907"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..>1303
                     /gene="groL"
                     /locus_tag="VFT30_06535"
     CDS             72..>1303
                     /gene="groL"
                     /locus_tag="VFT30_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5092323.1"
                     /translation="MAAKQLVFSEDARRKLKNGMNTVANAVGATLGPKGRNVAVDRKF
                     GSPTITHDGVSVAKEIELEDPFENMGAQLLKEAAQKTNDIAGDGTTTSTVLAHAIVNE
                     GLRALEAGYNPMLLKHGILQATESVVEELRKMSVKIDSREEIASVATNSAADEEIGNL
                     IADVMDKVGKDGVITVEESKSMQFEQEYVEGMQFDRGYLSAYFITNADQMQAVINEPY
                     ILINEKKISAAQDIVPILEKLVQIGKRELVIIAEDIDGEALATLVLNKLRGMLNVLAV
                     KAPGFGDRRKAMLQDIAILTGGTVIAEETGRKLETVTLQDLGRAEKVVSDKENTTLVG
                     GKGTKAAIEGRIKEIRAEIDKSTSDYDKEKLQERLAKLSGGVAIIRVGAATETEMKEK
                     KHRVEDALSAARAAVEEG"
BASE COUNT          333 a          353 c          376 g          241 t
ORIGIN      
        1 aagaaactgc tcatcctgcg cgaaagcgac attttagccg tgattgaagg ctaattctgg
       61 aggaatgaaa aatggctgct aaacaacttg tcttttcaga agacgcccgc cgcaagctca
      121 agaacggtat gaacaccgtc gccaacgccg tgggcgcaac tctcggcccc aagggccgca
      181 acgtggcggt ggatcgcaag ttcggttccc cgaccattac tcatgatggt gtctccgtgg
      241 cgaaggaaat cgagctcgaa gatccgtttg agaatatggg tgcccagctt cttaaagaag
      301 ctgctcagaa aaccaacgat atcgccggtg acggcactac tacatccacc gtgcttgctc
      361 atgccatcgt aaacgaaggc ttgagggcac tggaagctgg gtataacccg atgctgctca
      421 agcacggcat tttgcaggcc actgagtcgg tcgtggaaga actgcgcaag atgtccgtga
      481 aaattgactc gagggaggaa atcgcctcgg tcgcgaccaa ctctgccgcc gatgaagaga
      541 tcggaaattt gatcgcggat gtgatggaca aagtcggcaa agacggggtc atcaccgttg
      601 aagagtccaa gtccatgcaa ttcgagcagg aatatgtgga aggtatgcag ttcgaccgcg
      661 gttacctctc cgcctacttc atcaccaatg cggaccagat gcaggctgtc atcaacgagc
      721 cgtacatcct gatcaacgag aagaagatct cggctgccca ggatatcgtc cccattcttg
      781 aaaaactcgt ccagatcggc aagcgcgagc tcgtcatcat tgcggaagat atcgacggtg
      841 aggctctcgc caccctcgtc ctgaacaagc tgcgcggcat gttgaacgtg ctggctgtca
      901 aggcgcccgg cttcggtgat cgccgcaaag ccatgctgca ggatattgcc atcctgacgg
      961 gcggcaccgt gatcgcggaa gaaaccggac gcaagctcga gaccgtcacc ctgcaagacc
     1021 tcggtcgtgc cgagaaggtt gtctctgaca aagagaacac cacactcgtg ggcggtaagg
     1081 gcaccaaggc tgcgatcgaa gggcgcatca aggagatccg cgctgagatc gacaagagca
     1141 ccagcgatta tgacaaggaa aaacttcagg aacgtctcgc caagttgagc ggcggcgtag
     1201 ccatcatccg tgttggcgcc gcgaccgaga ccgagatgaa ggaaaagaag catcgcgtgg
     1261 aagacgccct ctccgctgct cgcgcagccg tggaggaagg tat
//
LOCUS       DASTWE010000626         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_986812, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000626 DASTWE010000000
VERSION     DASTWE010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986812"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..>1214)
                     /locus_tag="VFT30_06540"
     CDS             complement(69..>1214)
                     /locus_tag="VFT30_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEU5092324.1"
                     /translation="HECATKYLAGYHYGTYLPPLYTPLSANVVADNTRLIQHLLDQQC
                     RLANGSTPLVLLEMPPLTYFVAGTMSIPKFFRDVSEQAPCGLVLDVGHLWTVFRYSGA
                     HRAMPLAQFVEQFLNEFPMDRVVEIHVAGLAIHESHRTLASRPNGDMGDDALPAWIDD
                     HAAPIPNVLFEMLDQILCHPRLTSLKGLALEVDTKPVELIVDEFTEFSRHYGTLFSRL
                     GNAEQTVQDLEVRSMSKEQRSITPVMPALGAAYDRYARVLSGKTEPVGTEWKQDTASI
                     QDLNLYRSVYLPYEILHWGGKVEDMFVESCRRLRERDVSLEGFVVFWFSEPRPFSGIY
                     DFFLLKVERFVEFVREKAPDLHCIAQREAEELRCAYHFANELNVPEN"
BASE COUNT          298 a          326 c          316 g          274 t
ORIGIN      
        1 catcggtcac accatccatt ggggacaacc ctatgagcgg acggggaagg ctcatccaaa
       61 aggtcatatc agttctctgg aacgttgagt tcgttggcaa agtggtaggc gcaacgaagc
      121 tcctcggcct ctctttgcgc aatgcagtgc aaatccggcg ccttttcacg gacaaactcc
      181 acgaaccgtt cgaccttcag taaaaagaag tcatagattc cggagaaagg ccttggctca
      241 ctgaaccaga atactacaaa tccctcgagc gatacatcac gctctctgag ccgacgacaa
      301 gattctacaa acatgtcctc cacctttccg ccccagtgaa gaatctcgta agggagatag
      361 acagagcgat agaggttcag atcttggata ctggctgtgt cttgcttcca ttctgttcct
      421 actggctcgg ttttgcccga cagtactcgt gcgtatcgat cgtaggccgc tccgagtgct
      481 ggcatgacag gtgttatcga cctctgctct ttcgacattg aacgtacctc gagatcctgc
      541 acggtctgtt cagcattgcc cagccgggag aacagcgttc catagtgcct ggagaattcg
      601 gtgaattcat ccacgatcaa ttcaaccggt ttcgtatcca cctccaaggc aagccccttc
      661 aggctggtta gccgtggatg gcatagaatc tgatcgagca tctcgaacaa gacgttcggt
      721 atgggggcgg catggtcatc gatccaggca ggaagcgcgt catcgcccat atcaccgttc
      781 ggtcgtgaag cgagagttcg gtgtgattca tgaatggcca gacccgccac gtgaatctcc
      841 accacacgat ccatgggaaa ctcgttgaga aattgctcca caaattgtgc gagaggcata
      901 gctcgatgcg ctccggaata tcgaaatacc gtccagagat ggcccacatc caagaccagc
      961 ccacaaggag cttgttcgct gacgtcccga aaaaactttg ggatggacat cgtccctgca
     1021 acaaagtatg tgagtggcgg catttcaagc agcaccagcg gcgtgctccc gttggccaaa
     1081 cgacactgtt gatcgagcaa atgttggatc aatcgtgtat tgtcagccac cacgttcgcg
     1141 cttaacggag tgtagagggg gggaagatag gtcccgtagt gatagccggc gagatatttt
     1201 gtcgcacatt catg
//
LOCUS       DASTWE010000627         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_275372, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000627 DASTWE010000000
VERSION     DASTWE010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275372"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFT30_06545"
     CDS             complement(<1..405)
                     /locus_tag="VFT30_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5092325.1"
                     /translation="MGSVGTIRQGDSVKKIGHASLRRDDLNGKHDAPSPKALRQYLQR
                     FPQASVLVVGDLILDHYVMGRVSRISPEAPVPVVHVESESLRLGGAANVFNNILALGG
                     KADLCGVIGSDESGRLLMKELGNKRSSRGGVVI"
     gene            complement(366..1136)
                     /gene="lepB"
                     /locus_tag="VFT30_06550"
     CDS             complement(366..1136)
                     /gene="lepB"
                     /locus_tag="VFT30_06550"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5092326.1"
                     /translation="MSLEQNQRNVDKLSGSSRGGEQASLPGAKLADNTDRSGRKSIVR
                     EYAEAIIVAMLLAFAIRVFVVQAFKIPSGSMIPTLLIGDHILVSKLSYGLQWPTDCQL
                     QWTFPPVNCYTSKTVVAFGKPQRGDIIVFRFPEDEEKDFIKRIVGLPGDTVQLRNKVV
                     LVNGQPLDDKAFTQRIDPGVIDGAVNPRDNFGPVTIPEGSYFVMGDNRDQSLDSRFWG
                     YVREEKIRGKAFRIYWSWKGQGNWTEWVRWERFAKAIQ"
     gene            complement(1133..>2470)
                     /gene="lepA"
                     /locus_tag="VFT30_06555"
     CDS             complement(1133..>2470)
                     /gene="lepA"
                     /locus_tag="VFT30_06555"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU5092327.1"
                     /translation="DAADALPISAKEGKGVSEVLEAVIARIPPPSGNPEAPFKALIFD
                     SWFDNYQGVIVLARLVDGSIRPGMKIKVMSNDRTFEVMEVGQFTPKRTKKSELLTGEV
                     GYLCANMREVADVKIGDTLTDAVTPTAAPFPGYKEVKPLVFCGLYPTDTGRYEDLRDA
                     LVKLRLNDSSFVYEPETSLALGFGFRCGFLGLLHMEIIQERLEREYNLTLLTTAPTVV
                     YRVLTTKGEVLEVNNPTQLPPPSSIDSFEEPFILASIITPERYMGAILQLCQERRGIQ
                     QGLHFLDPTRVVISYELPLNEVILDFYDKLKSRTQGYASLDYEVLGYRDSDLVRLDIL
                     LNGEAVDALSFITHKDRSVQRGRQLAEKMKELIPRQMYEIAIQAAIGSKIVARETIGA
                     MKKNVLAKCYGGDITRKRKLLEKQKEGKKRMKAVGSVEVPQEAFLAILKVGEE"
BASE COUNT          534 a          731 c          642 g          563 t
ORIGIN      
        1 gatcaccacg ccgccgcggc tggaccgttt gttgccgagc tccttcatca gaaggcgtcc
       61 gctttcgtcc gatccgatca cgccgcagag gtcggccttt ccccccagtg ccaggatatt
      121 gttgaacacg ttagccgccc caccgagccg gagcgattct gattcgacat ggaccaccgg
      181 aaccggcgct tccggagaaa ttcggctgac ccgccccatc acatagtgat cgagaataag
      241 gtcgccgact acgaggaccg atgcttgcgg aaatcgctga agatactgtc ggagtgcttt
      301 gggcgacgga gcgtcatgct tcccattcaa gtcgtccctc cgaagagaag cgtggccgat
      361 tttctttact gaatcgcctt ggcgaatcgt tcccaccgaa cccactccgt ccaatttcct
      421 tgtcccttcc aggaccaata gatacgaaac gccttcccgc ggatcttctc ttcacgcacg
      481 tatccccaga accgactgtc cagactttgg tcgcgattgt cgcccatgac gaagtaagat
      541 ccctcgggga tggtcaccgg tccgaaatta tcgcgaggat tcaccgcgcc atcgataacg
      601 ccggggtcga ttcgttgtgt aaaagctttg tcgtcgagcg gctggccgtt cacgagcacc
      661 accttattcc gaagctgtac ggtatctccc ggcagcccga caatgcgctt gatgaaatct
      721 ttctcttcgt cctcgggaaa tcgaaacacg atgatgtcgc cacgctgcgg cttgccgaac
      781 gccacgaccg tcttggatgt gtagcaattg accggtggaa acgtccattg cagctggcaa
      841 tccgtcggcc actgaaggcc gtaagagagc ttgctgacca aaatgtgatc gccgatcagc
      901 aaggtgggaa tcatcgatcc tgatggaatc ttaaacgcct gcacgacgaa cacgcgaatg
      961 gcgaatgcca ggagcatcgc cacgatgatc gcttctgcat actcccggac gatggatttc
     1021 cgtcccgatc ggtccgtgtt gtcggcaagt ttggcgccgg gaagcgatgc ctgctccccg
     1081 cctcgcgaag aacctgacag cttatctaca tttctctgat tctgttcgag gctcattctt
     1141 ccccgacttt taagatggcc aggaacgctt cctgtgggac ctcgacactg ccgaccgctt
     1201 tcatccgttt cttcccttct ttttgctttt ccagcaattt ccgtttccgc gtaatgtcgc
     1261 caccgtagca cttcgccagc acattcttct tcatcgcgcc gatcgtctca cgcgcaacga
     1321 tcttgcttcc gatcgccgcc tgaatggcaa tctcgtacat ctgccgtgga atcagctcct
     1381 tcattttttc ggcaagctga cgcccacgct ggacggaacg atctttgtgc gtgataaacg
     1441 acaaggcatc gaccgcttcg ccgttcaaga gaatatcgag ccgtacaaga tctgagtcgc
     1501 ggtagccgag cacctcatag tcgagcgaag catagccctg cgtgcgcgac ttgagcttat
     1561 cgtaaaaatc gagaatgact tcgttgagcg gcagttcata actgatcacc actctggtcg
     1621 gatcgagaaa atgcaggccc tgctggattc cgcggcgctc ctgacagagc tggagaatgg
     1681 cccccatgta ccgctcaggc gtaatgatcg acgccaaaat aaacggctcc tcgaatgagt
     1741 cgatactgct gggaggtggc agctgtgtcg ggttattgac ctccagtact tcgcctttcg
     1801 tcgtcagcac acgatagacg acggtgggag cggttgtgag gagggtgaga ttatattcac
     1861 gttcgagccg ctcctgaatg atttccatgt gcaggaggcc caaaaatccg cagcgaaagc
     1921 cgaaccccaa tgcgagcgat gtttcaggtt catagacgaa cgaggagtcg ttcaaacgca
     1981 gtttgaccag cgcgtcgcgt aaatcttcat atcggccagt atcggtcgga tagagaccac
     2041 agaacaccaa cggtttgact tctttatacc cgggaaacgg cgcggcagtg ggggtcacgg
     2101 catccgtcag ggtatcaccg atcttgacat cggcgacttc tctcatgttg gcacagaggt
     2161 acccgacttc gccggtcaac agttcagact tcttggtccg cttaggtgtg aactggccga
     2221 cttccatgac ttcaaacgtg cggtcattcg acatgacttt gatcttcatg cccggccgaa
     2281 tcgaaccatc caccaatcgt gcaagcacga tcacgccttg ataattgtcg aaccaagaat
     2341 cgaagatcag cgccttgaag ggcgcctccg gattaccgga cgggggcggt atccgcgcaa
     2401 tgaccgcttc cagcacctcc gatacccctt tgccttcttt ggcactaatg ggcagggcat
     2461 cggcagcatc
//
LOCUS       DASTWE010000628         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_889936, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000628 DASTWE010000000
VERSION     DASTWE010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889936"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..963
                     /gene="flgG"
                     /locus_tag="VFT30_06560"
     CDS             172..963
                     /gene="flgG"
                     /locus_tag="VFT30_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEU5092328.1"
                     /translation="MIRAMWTAATGMTAQQINVDTVAHNLANVNTNSFKRSRAEFADL
                     LYQIQRLPGTNASNVGVFPVGIQVGAGVRPTTVAKEWMQGNMRQTNNELDLAIDGNGF
                     FQVSRPDGTIMYTRNGSFKRDNVGNLVTGDGDLLNPVITIPSGALKVDIGQDGTVSVL
                     LPGVTQASQIGQIQLTRFDNPSGLVAMGNNLFIDSFASGPPTQGTGGFTTGFGTIQQG
                     FLESSNVNLAEEMVNMIIAQRSYEINSKTIQASDEMMAIANNLRR"
     gene            974..>1773
                     /gene="flgA"
                     /locus_tag="VFT30_06565"
     CDS             974..>1773
                     /gene="flgA"
                     /locus_tag="VFT30_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEU5092329.1"
                     /translation="MSGFLPILIMLLLPGVFEVTTAIAGPSGESRAISSASRSPIHRT
                     ADTVVDQRAVSEVNPDLIRKTIQKHLENEWGRKVKTVHVTVLEPVDPVIIAGGTVELQ
                     VIPSPMEEGLGRRMFHVEAVANGKPRKTIQVVADIAAMIDAVVVTRFLKTDELIEMGD
                     LKTVGMRVHQVNHPFITDQGEVIGKSASRPLPPDTPLRPAFVKLPLVIKKGDRVLIEA
                     RRGGLSIRAYGVTKSSGQVGQTVMVSNLDSGRELRAKVVAPSLVEVEF"
BASE COUNT          420 a          457 c          509 g          387 t
ORIGIN      
        1 actatgaatc gacgcaaaag ctgattcaga cgcttgatcg aatggctgag gtcgcgattc
       61 aagacgtagg tcgagtgcta tgagacggaa tgcgtatggc cgatggcctc tagctgatga
      121 ggaacccctt gctatcagct atgtgcgatc agcccttctc tggaggattc tatgattcga
      181 gctatgtgga cagcggcgac cggaatgacg gctcagcaga tcaacgtcga caccgtcgcc
      241 cataaccttg ccaacgtcaa caccaattcc ttcaaacgca gccgcgctga gtttgcagac
      301 ctgctgtatc aaattcagcg actcccaggc acgaacgcat ccaacgttgg agtctttcct
      361 gtcggtatcc aggtgggggc cggcgttcgt ccaacgacgg tggcgaaaga atggatgcaa
      421 ggcaatatgc gccaaacaaa caatgagctg gacttagcga tcgacggcaa cggctttttc
      481 caagtgtctc gtcccgatgg aacgatcatg tacaccagaa atggttcctt caaacgcgac
      541 aacgtgggca accttgtgac cggcgacggc gatcttctga atcccgtgat tacgatccct
      601 tccggcgcac taaaggtgga tatcggtcag gacggcacgg tctccgtatt gctgccagga
      661 gtcactcagg cttcgcaaat tggacaaatt caactcacgc ggttcgacaa cccttccggc
      721 ttggtcgcca tggggaacaa tctctttatt gacagctttg cgtcaggacc tccgacgcag
      781 gggacaggcg gcttcaccac cggatttggc acgatccagc agggattttt ggagagctcg
      841 aacgtcaacc tcgcagaaga aatggtcaac atgatcatcg ctcagcgaag ttacgagatt
      901 aactccaaga cgattcaggc ttccgacgaa atgatggcca ttgccaataa tctcagacga
      961 taaagagcat cgtatgtctg gattcttacc gatcctgatc atgctccttt taccgggagt
     1021 cttcgaggtg acgacagcca ttgcggggcc cagtggcgag tctcgcgcga tttcgtctgc
     1081 gtcccgaagt ccgattcatc ggacggccga caccgttgtg gaccaacggg ctgtcagcga
     1141 ggtcaatccc gatctaatta ggaagaccat tcagaaacat ttggaaaacg agtgggggcg
     1201 taaggtcaag acggtgcatg tgaccgtcct ggaacccgtt gatccagtga taatcgcagg
     1261 cgggacggtg gagttacagg tcattcccag ccctatggaa gaaggcctag gacggcgcat
     1321 gtttcatgtg gaggcggtgg cgaacgggaa accgcggaag accatccaag ttgtggccga
     1381 tattgcggcg atgatcgatg cggtcgttgt tacccgcttt cttaagaccg acgaactgat
     1441 tgagatgggc gatctcaaga ctgtcggcat gagagttcat caggtgaatc accccttcat
     1501 taccgatcaa ggtgaggtca taggaaagag cgcttcgcga cctcttccgc ccgatactcc
     1561 gctgcgtccc gcctttgtga agcttccgtt ggtgatcaaa aagggtgatc gagtcctcat
     1621 cgaagcgcgg cgaggaggac tgtcgattcg ggcctatggt gtgacgaaat cgagcggaca
     1681 ggtcgggcag acggtcatgg tctccaatct ggattccggc agggagttga gagccaaagt
     1741 ggtggcgccc agtctcgttg aggtggagtt tta
//
LOCUS       DASTWE010000629         1332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_534371, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000629 DASTWE010000000
VERSION     DASTWE010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534371"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VFT30_06570"
     CDS             <1..971
                     /locus_tag="VFT30_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HEU5092330.1"
                     /translation="SKDADLSMDGYVYDAGSKEVVGGKRYVGSTSVARLMAHRFADEL
                     VFRYTGEPGIARTKIAYVSELGTARELFVMDYDGYDPRQLTADGFLNLMPRWSPDRRF
                     LVFTTYRNRNTQDIDMIELATGKRWTLVAQSGLNITPALSPDGNALAYSSSHEGNAEL
                     YRLDTRTKAVQRLTTNAAGDLSPSWSPSGRELAFTSDRSGGPQIFLMSADGSNVRRLT
                     FDGDYNAAPAWSPRGNWIAYVCRTPKKEYKLCVVTPDGQKHLQLTTGLGVDDSPSWSP
                     DGRHLVFSSTVDGKSQIYMIDADGKDLERITFTGTHNSAPSWSPAS"
     gene            1051..>1332
                     /locus_tag="VFT30_06575"
     CDS             1051..>1332
                     /locus_tag="VFT30_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092331.1"
                     /translation="MKTLPASYLPLILGLIVLGIPACSKKAVRSGGDTQALQEEMARG
                     EMAKGGAAKEGASSSFPDTSFSGRDDSNGLRGLDRNPSEERLGGNAGGPG"
BASE COUNT          310 a          327 c          406 g          289 t
ORIGIN      
        1 gaagcaagga cgccgatctc agcatggacg gttacgtgta tgacgccggc agcaaggagg
       61 tcgtaggcgg gaaacgatat gtcggctcga catcggttgc ccgtctcatg gcccatcgtt
      121 ttgcggatga gttggtcttt cgctatacgg gagaaccggg aatcgcacgg acgaagatcg
      181 cctatgtctc cgagctgggg acggctcgcg agctattcgt gatggattac gatggatatg
      241 atccgcgcca gttgacggcc gacgggttct tgaacctgat gcctcgttgg tcgccggatc
      301 gtcgattttt ggtcttcacg acgtaccgca atcggaacac gcaggatatc gatatgatcg
      361 agcttgccac gggaaagcgt tggacacttg tcgcgcaaag tggattgaac atcactccgg
      421 cattatctcc cgatgggaat gcactggcct attcgtcaag ccatgaaggc aacgccgaat
      481 tgtaccgttt ggatacccgc acgaaagccg ttcagcgact gaccacgaat gcggccgggg
      541 acttatctcc ctcatggtct ccctcgggtc gggagctcgc ctttacgtcc gatcgaagcg
      601 gaggaccaca aattttcctc atgagtgcgg atggatccaa cgtgcgtcga ttgacgtttg
      661 atggagacta taatgcggcg ccggcctggt cgcctcgagg aaattggatc gcctatgtat
      721 gccggactcc taaaaaagag tacaaattgt gtgtggtcac ccccgatggt cagaaacatc
      781 tgcaattaac gacaggactg ggagtggatg attctccatc ctggtctccg gatggacggc
      841 atcttgtctt cagctcgacg gtggatggta agagtcagat ctacatgatc gatgcggatg
      901 ggaaggacct cgagcgtatt acgtttaccg ggactcacaa tagcgcaccg tcctggtccc
      961 cggcatcgta aatatccagg aaggcattga cggcaatcgt ccttcgctgt aagaaaaagg
     1021 gacgatattt cacaggaggg aaggaatcca atgaaaacgt taccagccag ctacttgccg
     1081 ttgattcttg gtctcatcgt actgggaatt ccagcgtgct cgaagaaggc ggtccgatca
     1141 ggaggagata cccaggcttt acaagaagaa atggccagag gagagatggc caagggagga
     1201 gcggcgaagg agggggccag ttcaagtttt cccgatacgt cgttctccgg acgtgatgat
     1261 tcgaacggct tgcgggggct ggatcgaaat ccttcggaag agcggctggg cggaaatgca
     1321 ggtggcccag gc
//
LOCUS       DASTWE010000630         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1407314, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000630 DASTWE010000000
VERSION     DASTWE010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407314"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1037..>2049
                     /locus_tag="VFT30_06580"
     CDS             1037..>2049
                     /locus_tag="VFT30_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092332.1"
                     /translation="MPWDQVQELVISIIPTQTDQPIEENAESVVDRSTCEAVSVPNVG
                     DDQAQPALSPIGSAAETETFSIAPDRQVSPELQIATADFAQASVQTEGDFKFVEPEQP
                     LPGVSSLTMDEPLALEAPMAAVASSIFESRPVADHMVVASPEVTPQSADSVLCADNDR
                     ETPAVLSIVQEAPVIPSNPASEALSKETEEIPIFQPLQKQQEIYELPPETAATPQPVE
                     ETTEVQTYERQPQTVALVEARPSIPELVTEPITESVPEPAPEQEEWVKAEESIRFIEP
                     PQSSPIAHVLPSISERKEATRSMSVAAAAVDVLFESSRNVRVTETRDPVAESTPVRRS
                     NSTL"
BASE COUNT          507 a          545 c          535 g          462 t
ORIGIN      
        1 aagtcagggt atccagactg gtcttgaacc gatggatgag gtaatgccgt gggagattca
       61 ggattctcaa acgacgccat cggatgagtt cagggtagcc gatgtatcta gttcatcggg
      121 gtctctggcc ccacccgtga tcgttgacct ttctgaacat caaggaccgc tcgatgaaaa
      181 gttggtgcct tctgagccac tctttcgaga gcagacggct agtagcgcgg gtgaattgca
      241 aagtgatgca ccggaaggac ctcgtacaca taaatttgag agtgctgctt ctggagtcca
      301 ggtggatgat ttttccagac aagtgggttc tgaaagttcg catccatccg ctctctcatc
      361 agtgctcgct gctgagagct ccgagaaaca atctgggcca gattcggccg tatcaacagg
      421 tggtccggtc gcagttcagg aacaagacag gttgttgacc gaggctgaac cagagaagcc
      481 ctatgatgta agtggctcat tcacggtttc cgcacctacg gagtctctca atacttcaga
      541 acctccgatt agctcgtcac agcttatcga agctgccggc gtaatacctt catcccagat
      601 tgaagagatg tcagcgcccg ccttcctagg catggaaccg tcgactgccg aaccgataga
      661 ggctgaggat cgacaaccct cccttcagca ccctctcgag actaattcaa aggagatggt
      721 tgctccatct ccagacgtcc gcactccatc gaatcccgag tcggtttcag cccatgagat
      781 ttcagaacca tggaaacaac ccggcttctc atgggaatct gtgtttaatc atgcatggaa
      841 gttcggaagt gatcgctcag caaatgtagc tccgtcggga ataaccaagg cgaacgtcga
      901 ggaatcacct tctccagcgc cagtagcgtc acctctccag gaacaaatat tagaaaacca
      961 agcggttgag attcccgagc gtgaaacttc gatgtcgctt ggagaccaaa cctcttctgg
     1021 atctccaatt gctccgatgc catgggacca ggttcaggag ttggtcattt cgatcatccc
     1081 gacgcaaaca gatcagccga tcgaggaaaa cgcggagtcg gtggtagacc gatcaacttg
     1141 tgaagctgtc tcggtaccga acgtcggtga cgatcaagcg caacctgcgc tttctccgat
     1201 tggttccgct gcagaaacag agacgttctc gatcgctcct gatcgacagg tctcgccaga
     1261 gctgcaaatt gccactgctg attttgcgca agcctctgtt cagacagaag gggactttaa
     1321 atttgtagaa ccggaacagc cgcttccagg ggtgagctct ctgacgatgg atgagccgtt
     1381 ggcgttggaa gcccctatgg ccgcagtagc atcctcgatt ttcgaaagta gaccggtcgc
     1441 agaccatatg gtggtggcat ccccggaggt tacgccgcaa agcgctgatt ctgttttgtg
     1501 tgcggacaat gaccgtgaga cccctgcagt tctgagtatt gttcaggaag ctcccgtcat
     1561 cccctcaaac cctgcaagcg aggcgctgag caaagagacc gaagagattc caattttcca
     1621 gccgttacaa aaacaacagg aaatttatga actgccgcct gaaacagcgg caactcccca
     1681 gcccgtcgaa gagacgaccg aggtgcagac ttatgaacgg caaccgcaga cagtagcgtt
     1741 ggttgaggcg cgaccttcta ttcctgaact ggttactgaa ccaattactg aatcagttcc
     1801 tgaaccagcg cccgaacaag aggagtgggt caaagcggaa gaatcaattc gatttatcga
     1861 gccacctcaa tcctctccca ttgcccacgt gcttccgtcg atttcagaac ggaaggaagc
     1921 cactcggtct atgtcggtag ctgcggctgc tgttgatgtg ctttttgagt cgtctcgaaa
     1981 tgtcagggta actgaaacac gagatcctgt ggcggaatca acgcccgtcc gaagatccaa
     2041 ttctacgct
//
LOCUS       DASTWE010000631         1160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_954398, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000631 DASTWE010000000
VERSION     DASTWE010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954398"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..555)
                     /locus_tag="VFT30_06585"
     CDS             complement(118..555)
                     /locus_tag="VFT30_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEU5092333.1"
                     /translation="MPQPEKLFKTLGGATVFGRRQVQSTDDVRAMIHRGLPYRSLEIL
                     ASTFHLDLPRIGKILSVPPRTMARRKAEQRLTAQESDRLARLARILTYATDVFGTEEK
                     AAMWISRSNRILQGTPPIELLDTDLGTQVVETMLGRIEHGVIS"
     gene            722..1060
                     /locus_tag="VFT30_06590"
     CDS             722..1060
                     /locus_tag="VFT30_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092334.1"
                     /translation="MKKSKAYQPELIESLRNPRESEKYLNVALEEDDPELFLLALRKC
                     DGSTRRCSAACRENEKLNRESIYKMLSERGILNSRASMCYCTRWVFGWPSRPIDEQAS
                     LLPETEMLPY"
BASE COUNT          288 a          306 c          308 g          258 t
ORIGIN      
        1 ataacgtacc cgacgtatag atgacgggag ttccaggccg attccaccgt cccccgtagc
       61 gtcgagctcc ttctccgtct gaagacgcat gccccttcag agtcaggcgc cagacaacta
      121 ggatataaca ccatgttcga tgcgtcccaa catcgtctcg acgacttgcg tgccaagatc
      181 ggtatcgagg agttcaatgg gtggggttcc ttggaggatt cgattcgagc ggctgatcca
      241 catggcagcc ttctcttccg taccaaagac gtcggtggca taggtcagga ttctcgccag
      301 gcgcgccaag cggtccgatt cttgtgcggt gagacgctgt tcggccttgc gccgagccat
      361 ggtccgagga ggcacactga ggatctttcc tatgcgcggg agatcaagat gaaatgttga
      421 ggcgaggatt tccaaggaac gataaggaag ccctcgatga atcatcgcgc gcacgtcgtc
      481 agtcgactga acctgccttc gtccgaagac cgtggcgcca cccaatgttt taaagagctt
      541 ttcaggttgt ggcatgactt cctttgtcac ctggcttaat agtattgcca gatggcacca
      601 ttgtcaagat aacgatacag cgcctatcgc tgtatgggtg cataacgaac cgcctgaaca
      661 ctccctgggt tgccatgtag tagtttataa ggctacacgt atgtacttga atgaggtgat
      721 catgaaaaag agcaaggcct atcaaccaga actcatcgag agcttacgca atccgcgaga
      781 atcagagaaa tacctgaacg tggcgctgga agaagacgac cccgaattat ttttgctggc
      841 ccttcggaaa tgcgacggaa gcacaaggag gtgtagcgcg gcttgccgag aaaacgaaaa
      901 actgaaccgt gaaagcatct acaagatgct ctccgagcgg ggaatcctga actcaagagc
      961 ctcgatgtgt tattgcacgc gttgggtttt cggctggccg tcacgtccca tcgatgaaca
     1021 agctagctta ctgccggaga ctgagatgct accgtattaa cttgtcatat gggttccaat
     1081 ccaaaaccaa acaattccgg ctggtgcctt accccacagt ccgatattgt ggtccgactc
     1141 gtgcagacca aaaccgaccc
//
LOCUS       DASTWE010000632         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_890032, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000632 DASTWE010000000
VERSION     DASTWE010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890032"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFT30_06595"
     CDS             complement(<1..321)
                     /locus_tag="VFT30_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092335.1"
                     /translation="MRLSRFFHIPALVLLFVLTISGQQTNPVDRRVANPITDTPNINP
                     VSSQQDIKAPKSVRPVVTPEGGDGEVVVYSENQSVEGEAGKRIVTHTGNVDVRYGIYR
                     LQADK"
     gene            400..>1106
                     /locus_tag="VFT30_06600"
     CDS             400..>1106
                     /locus_tag="VFT30_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5092336.1"
                     /translation="MAKQKVLVVEDEELMRTIVRTLLEGEGYEVFSADSAEAALRIFP
                     TNDIAVTLTDIKMAGMDGLALLDQIKAIDEHSIVIIMTAFSSVDSAIAALRKGAYDYV
                     TKPFVNEDLLQRVRNAIQNRVLFTENRALRRELNRQYNFPEIVGSSEKMQRVFELVKK
                     VADTTSNVLILGESGTGKELIARSLHFNSSRSSKPFLAVNCGALPESLLESELFGHTK
                     GAFTGAVADKKGLFRSA"
BASE COUNT          265 a          295 c          309 g          237 t
ORIGIN      
        1 cttgtccgcc tgcagccgat agataccgta gcgaacatcc acgttaccag tgtgcgtcac
       61 gatccgcttc cccgcctcgc cctcaaccga ttgattctcc gaatagacga ctacctcacc
      121 atcgccgccc tcgggtgtaa cgacgggccg tacggattta ggggctttga tatcctgctg
      181 ggaggaaacc ggattgatgt ttggcgtgtc ggtgatcggg tttgcgactc tacggtcaac
      241 aggattcgtc tgctgtcccg agatggtcag gacaaacagc agtaccaacg ccgggatatg
      301 gaagaatcgt gacaaccgca tcaggcattc tgaggagaat gattgatgct aacacgcgaa
      361 aagaagcgtg ccaaaggcat gtggtaaact gccgtaaata tggcgaaaca aaaggttttg
      421 gtcgtcgagg atgaagaact gatgcggacc atcgttcgga cgcttttgga aggtgagggc
      481 tacgaagtct tctccgcgga cagcgctgaa gcagctcttc gaatcttccc gaccaacgac
      541 atcgcggtca ccctaaccga catcaagatg gccggcatgg acggacttgc actcttggat
      601 cagatcaagg cgatcgacga gcattcgatc gtgatcatta tgacggcttt ctcgtccgtc
      661 gattcggcga tcgccgccct tcgcaagggt gcgtacgatt acgtcacaaa acctttcgtc
      721 aacgaggatc tcttgcagcg cgtgcgcaac gcgattcaga accgtgtcct cttcaccgag
      781 aatcgagcgc tccgccggga actgaatcgg cagtacaact ttcccgagat cgtgggttcg
      841 agcgagaaaa tgcagcgagt attcgagctg gtgaaaaagg ttgccgacac gacttcgaat
      901 gtactgattc ttggcgaaag cggaacgggg aaagaactta tagcccgctc gcttcatttc
      961 aatagcagcc gatcatcgaa gcctttcctc gccgtgaact gcggggcctt accggaatcc
     1021 ttgctggaaa gcgagctttt cggccatacg aaaggggcct tcacaggcgc agtagctgac
     1081 aagaaggggt tattccgatc cgcgga
//
LOCUS       DASTWE010000633         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_275609, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000633 DASTWE010000000
VERSION     DASTWE010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275609"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..>1016
                     /locus_tag="VFT30_06605"
     CDS             206..>1016
                     /locus_tag="VFT30_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5092337.1"
                     /translation="MLIDTTLLSETEQRFAERQTTREEGVRLIRTGNLLQADSPERVQ
                     ARLKRLGIDRRVAERVAATKAGFWSEAKRGIVDSVEQAKAPGSMEQEKVEAVMAETLG
                     IEPEVMGPAEMEVNELLLERIVGRNDLLGIAYMDRGLAASRTVGCVRLFDNGRCVGSG
                     TGFMVSPRLLLTNNHVLEDAAMGRQARVVFNFQDDPYGNALSPITFSLDPDAFFLTSQ
                     PLDFTLIAVGRPAAEGTVDLEEIGWSRLIETEGKIINGEYVTIIQHPNGQPK"
BASE COUNT          228 a          271 c          304 g          213 t
ORIGIN      
        1 accatctatg ggcctctatt cgtcaccgga gattccactt ctctgtcgct gctcactgtc
       61 ctcaatctct tcctgctgag cttcagcatg ttttcattct tgattcccca tcacgaaagt
      121 acttagatag accggctgcc aaagcgtagc tatcgatacc gtccaatgac tgcaaggcac
      181 aggtcctgtg ccgaagggag gaacgatgct gattgacacg accttactga gcgagaccga
      241 acagcgattt gcagaacggc agaccacgcg tgaggaaggt gtccgactca ttcgtacagg
      301 gaatcttctg caggcggact cgccggagcg ggtacaagcg agattgaaac gcttgggaat
      361 tgatcgacga gtggcggagc gggtggccgc cacaaaagcc ggtttctgga gtgaagcgaa
      421 acgaggaatc gtggacagcg tcgagcaggc aaaggccccc ggcagcatgg aacaggagaa
      481 ggtggaagca gtgatggccg agacactggg gatcgagcca gaggtcatgg gccccgcaga
      541 gatggaggtc aatgaattgc tcttggagcg gatcgtcggg cggaatgatt tgttgggcat
      601 tgcctacatg gatcgcggcc tcgcagcaag tcgcacagtc gggtgcgtac ggctctttga
      661 caacgggcgg tgtgtgggtt ccggtaccgg cttcatggtg tccccacgac tgctgctgac
      721 caataatcat gtcctcgaag atgcagccat ggggcgccag gcgcgcgtcg tcttcaattt
      781 tcaggacgac ccctatggca acgccttgtc gcccattaca ttttcactcg accccgacgc
      841 gttcttcctc acgagtcaac cgttggattt caccctgatt gccgtgggtc ggcctgcggc
      901 ggagggaacg gtcgatcttg aggagatcgg atggtctcgc ctgatcgaga ccgaaggtaa
      961 gatcatcaat ggtgaatatg tcacgatcat tcagcatccg aacggccaac ccaaac
//
LOCUS       DASTWE010000634         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_534458, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000634 DASTWE010000000
VERSION     DASTWE010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534458"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VFT30_06610"
     CDS             <1..1151
                     /locus_tag="VFT30_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5092338.1"
                     /translation="GADVILVERFGYLGGLATGGLIILLLTLDDGRGRQVIGGLCQEV
                     TDRLAKRGAAMFPSKSEWGSADEKLIQRDQSWGLVWGHGPHRVRYSVAYDPEEFKFAL
                     NNMVEESRVKLLLHSYACEAIIENGKITGVTFQSKSGRFAILAKAVVDATGDGDVFTS
                     AGAAYESEEVLPWLWFAMGGVENADAAIQSGGWFFHTIGEGKVLLPWGAAERVTRKID
                     ATKPEDLTYAELECRKRVMEVVDRLRKETPGFANAHLCHIADQLGITESRRLVGEYVV
                     GRDDIDTTCEDVIAITGHWTKYEALYYIPYRSLLPKEFSNLLVAGRCISVDHRVHHAT
                     KEIPPCMATGEAAGTAAALAVRSGIDPKMLNVNKLQQTLQERGAILRI"
BASE COUNT          268 a          275 c          372 g          289 t
ORIGIN      
        1 acggtgccga tgtcatactg gtcgaacgtt tcggttacct tgggggtctg gcaacaggtg
       61 gccttattat cctgctgttg actcttgatg acggtcgtgg ccgacaggtc atcggcggtc
      121 tgtgtcagga agtgacagac cgattggcca aacgtggcgc ggcaatgttt ccgtcgaagt
      181 ctgaatgggg gagtgccgat gaaaaactaa tccaacgtga tcaaagttgg gggttagtct
      241 ggggccacgg gccccaccgg gtccgttatt cggtggcgta tgatcctgag gagttcaagt
      301 ttgcgttgaa taacatggtc gaagagtcga gggtaaagct cctgcttcat tcgtacgcct
      361 gcgaggccat tatcgaaaac ggaaaaatca caggagtgac cttccagagc aaatccggcc
      421 gctttgcgat tcttgccaaa gcggtagtcg atgcaaccgg cgatggcgac gttttcacct
      481 cggccggggc tgcatatgag agcgaggagg tcctgccgtg gctatggttc gcgatgggcg
      541 gtgtcgaaaa cgcggacgct gcgattcaat caggaggctg gttttttcac accattggtg
      601 aaggaaaagt tctactgccc tggggagcgg cggaacgggt cactcgcaaa attgacgcta
      661 cgaagccgga agatttgact tatgctgaac tcgaatgtcg caagcgggtg atggaagtgg
      721 ttgatcgctt acgcaaagaa acgcctgggt ttgctaacgc ccacctctgt catattgccg
      781 atcagcttgg cattactgag agccgccgtt tagttggtga gtatgtggtc ggacgtgatg
      841 acatcgacac gacgtgcgaa gatgttatcg cgattaccgg acattggact aaatacgaag
      901 cgctgtacta cattccgtat cgttcattgt tgccaaaaga attttctaat ctgttagttg
      961 ctggccgctg tatttcggtt gatcatcggg tgcatcacgc gacgaaagag attcctccct
     1021 gcatggcgac tggtgaggcg gcaggcactg ctgcggcact cgcggtccgg tcggggattg
     1081 acccgaagat gctcaatgtg aataagctcc aacagacgct ccaggaacgt ggagcgattt
     1141 tgcggatcta aggacgtgac gcgtgatgtg tgctgcgtga agaaaaggaa gtgttccttg
     1201 cttc
//
LOCUS       DASTWE010000635         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1019324, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000635 DASTWE010000000
VERSION     DASTWE010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019324"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..775
                     /locus_tag="VFT30_06615"
     CDS             368..775
                     /locus_tag="VFT30_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5092339.1"
                     /translation="MMARVTGIGGVFFKSKNDSAALRAWYQKHLGMSLESFGGAILRW
                     PDDKAEDKGLTVWHVAEKESQWFSPSDSSFMINYRVDNLDELIAQLRADGVEIIQGPE
                     SAENGKFAWILDLDGNKVELWEPMIWDDKNKSA"
     gene            1023..1727
                     /locus_tag="VFT30_06620"
     CDS             1023..1727
                     /locus_tag="VFT30_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU5092340.1"
                     /translation="MSDIFISYASPDRARAERLKAWFEEAGWSVWIDRAIDLGEGWEQ
                     QIQNELDRAALVVVLWGPAARRSEWVQREASVAQQSGRLLQVHATGLPLLPPFDVLQA
                     VRMQSWSGEAGHSERVKLLRAVADRLGKPLPPHLNVTTPDEALTAYHEEISEALELAF
                     YYCARQVERVRLQRERGYGLASDFEEIGSSFTAMLALLRTDEGTTDDREGILHRLMED
                     FLNQLLLLAPDPNALT"
BASE COUNT          427 a          464 c          508 g          413 t
ORIGIN      
        1 tcagctaaga gaccatcgga atcgacaacg tgcggcaaat ttcaaactga gacactacca
       61 aattttgatg ccatcagggc gcacctacgc tatcgggtaa ggtactgacc tcctccaagc
      121 acggttctcg ttacacagta ctggcttact tttttcatcg cttacaccct ccgtttctag
      181 ttttgaatta tgaatcgcgg tgcagaatga caaactacta cagtgcgcac acttctaatg
      241 tccaaacatg tgattgcatt ttcaggctga tgtgaggcgt gctccacggt tagttcttgt
      301 ctcacgattc gggaaggtgt aagttgcatc cgctgcacct actagcatcg acaatcgcgg
      361 aggatgaatg atggcgagag ttaccggaat cggcggagtc ttctttaaga gcaaaaacga
      421 cagcgccgcg cttcgagcct ggtatcagaa acacttgggc atgtcgctcg aaagctttgg
      481 tggcgcaatt ctgagatggc cggatgacaa ggccgaggac aaggggctta ctgtatggca
      541 cgtcgcggaa aaagagagcc agtggttcag cccgagcgac tcgtctttca tgatcaacta
      601 tcgggtagac aatctcgacg agttgattgc ccaactccga gcggacggag ttgaaatcat
      661 tcaaggcccc gaatccgccg aaaacgggaa gttcgcgtgg attctcgatt tggatgggaa
      721 caaagtggag ctgtgggagc cgatgatctg ggacgacaag aacaagagtg cctgaagttc
      781 cgcccgagcc gccatcaatt caattagtgc cgtgttcttc ctgtctccca ctgtaacggg
      841 caaatactcc taaccccatc tgaagtccgc gtatctgttg gacacagtca gtaccacctg
      901 cgatagcggg tgggtgcgta gcagtgggcg tctccggaat catttcgtat cttgagcaat
      961 gctgaggaca ttcgtgcatt gtggtgagcc ttggagctgt acgcactcag accggaggtt
     1021 ttatgtcaga tatatttatc agctatgcaa gtccggaccg tgcacgggcc gaacggctca
     1081 aggcgtggtt tgaggaggcg ggatggtctg tctggatcga tagagcgatc gatcttggcg
     1141 aaggatggga acagcagatc caaaacgaat tggatcgtgc tgccctcgtg gtcgtgctgt
     1201 ggggaccagc ggcgcgccga tcggagtggg tacagcgaga ggctagtgtc gcgcagcagt
     1261 ctggtcgtct attgcaggtg catgcgaccg ggctacctct gctgccaccg ttcgacgtcc
     1321 tacaagctgt gcgtatgcaa tcctggtcag gtgaagccgg ccactcggag cgagtgaagc
     1381 ttctgcgagc tgtggcagat cgacttggta agcctcttcc accgcacctg aacgtcacca
     1441 ctcccgacga agctctcacc gcgtatcacg aagagatctc cgaggctctc gaactggcct
     1501 tttactattg cgcgcgacaa gttgagcggg ttcgtcttca acgcgaaagg ggatacggcc
     1561 tggcttccga tttcgaagag atcggaagta gtttcacggc aatgcttgcc ctgctgcgaa
     1621 ctgacgaggg gacgacggat gacagagaag gtattctcca tcgattgatg gaagacttct
     1681 taaatcaact cttattacta gcgccagatc caaacgcact cacgtagctc gcgcacgacg
     1741 cgtaagctgc gactcaaagg cctaggatgg cacttgcagc ctctacatca gacggtttca
     1801 acaacccatt cc
//
LOCUS       DASTWE010000636         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1439463, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000636 DASTWE010000000
VERSION     DASTWE010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439463"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            651..1304
                     /locus_tag="VFT30_06625"
     CDS             651..1304
                     /locus_tag="VFT30_06625"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5092341.1"
                     /translation="MDNDAAYGVWWLVLVNSIFFIVFALSFTRPRTARDWRSLGVFSA
                     FIVALFAEMYGFPLSIYLLSGWLASHYPEVDPFAHDTGHFWHTLLGKEGPAHSDPLHI
                     FSEVLVFAGLILLALSWRVLYRAQQARTLATTGPYALMRHPQYTAFMMVMLGFLLQWP
                     TIPTLLMFPVLVGMYILLAYEEEAEARAQFGEAYDRYAAVTPGFSPSWRKRVRKPEG"
     gene            1462..1713
                     /locus_tag="VFT30_06630"
     CDS             1462..1713
                     /locus_tag="VFT30_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092342.1"
                     /translation="MAPQPYVGPRRRLALARAAALKEFTEENDWKKALQDLTMAASHY
                     VDRMSKTRKTEKSLKVLRDAIAQAERVMAIEGAKGTRPL"
     gene            1786..2661
                     /locus_tag="VFT30_06635"
     CDS             1786..2661
                     /locus_tag="VFT30_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092343.1"
                     /translation="MTASYRRFMVCGLSVLAWCWCIAGGTAAHEPVATSQSGNSDSVQ
                     HRSDIAWDAIEWTERRYKIEALGFKARDETGNSWLGSDEVMIRTDDAKGWTVSGQIGD
                     IDSGDAHHFDPAKSCIAPVRPGIVVLGKTSVCDDSGEAAPLSFAVEFWEKDWFGGFGR
                     DFCSPGSPASGTHAGPHCANDNAGDDFIGRAHIDLSIQDLETALLNVGDEYIETVVLE
                     QCGSGSTCDVSYGPDYSFTYRITRLRDARVGLRPMLDKAMGTIGTHSELDAIAAGLRS
                     LRAPSPRQIEPGMGK"
     gene            2784..>2992
                     /locus_tag="VFT30_06640"
     CDS             2784..>2992
                     /locus_tag="VFT30_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092344.1"
                     /translation="MKYDVHWSVLRNTLSILLLALLAGCGTMSGLSCGADGQLAVQEL
                     LYFGTETPSGHVTPEAWTQFLSEIVT"
BASE COUNT          677 a          757 c          881 g          677 t
ORIGIN      
        1 tcacctcctt gtccggtaat tttttgactt ttggtgtctt cagcgcacgg cgccatgtga
       61 gcctgatccg taaggcttca ggatagaaca ctgaaacctg aaggcctcag cctcactcac
      121 cggcgtacca caatcgcact aaggtgatga aaaggactac gtttgaggag gatgaaagac
      181 ggaggtcacg aagaccgaaa gatggaccac gggttcgcgc ttctcctgaa gaccggaacg
      241 acttgccaat tctggttcag gtgtgatcgg agggattgaa aagtcttcag aaactgattc
      301 cgccggtatg ctcgaagtga ggagtccaac aagcgtaacg ggtgtcccca gcatttgcga
      361 caacacgcag atacaaagga gaatgaccag gaatgcccgg ccagttcccc gagacgaaga
      421 gaagaatgaa gttgtgccga tcccccgcat gaaaatcact atactcccct agggacaagc
      481 gagcaagcga gctcagatcc tttcttgtct ggacaatgcc ggaaggaaga caggcgttct
      541 ctatgagggt tcactgactg ctctggctgt atgcggtatg ttgtagcagt tgcaggaact
      601 gccgcctttc tatttcggga catgtcacac gacgggcaga gagacgacat atggataacg
      661 acgcagccta tggagtgtgg tggctcgttc tcgtcaattc tattttcttt atcgtcttcg
      721 cgctgagctt cacgcgtcct cggacggcgc gcgactggcg atccctcgga gtcttctccg
      781 cgtttatcgt ggccttattt gcagagatgt atgggtttcc tctgagcatc tatctgcttt
      841 cagggtggct ggcgagccat tatcccgagg tcgatccgtt tgctcacgat accggccact
      901 tctggcacac cttgttgggt aaagaaggcc cagcgcattc cgatccgttg catattttca
      961 gcgaggtgct ggtctttgca ggactaatct tgctggctct ttcctggcgc gtcttgtatc
     1021 gagcgcaaca agcccggact ctggctacga ccggacccta tgccttgatg agacatccgc
     1081 agtacacggc cttcatgatg gtcatgctgg gctttctgct ccaatggccg accattccaa
     1141 ccttgctcat gttccctgtg cttgtgggga tgtacattct tctggcatac gaagaggagg
     1201 ccgaagcccg tgcacaattc ggtgaggcct atgaccgata tgcagcggtt acgccaggct
     1261 tttccccttc atggagaaaa cgggtacgaa aacccgaagg ttgaacgtaa gaaggccggg
     1321 tgtatggata tggtcagact catgaggtga aatgacacga ggggtgctct gtaaccttcg
     1381 gcgagcttat ggtacgtttg cttccttcct ggagaaaata caatgatatt tagacggccg
     1441 gagctcgaaa aagataatcc gatggccccc caaccatatg tgggcccgcg taggcggctg
     1501 gcgctggctc gagccgcagc attgaaggaa ttcacggaag agaatgattg gaaaaaggcg
     1561 ttgcaggatt tgacgatggc ggcatcccac tatgtggatc gtatgtcgaa aaccagaaaa
     1621 acagaaaaga gcctgaaggt attgcgggat gcgattgcac aagccgagcg agtgatggca
     1681 atcgaaggcg ctaaaggaac cagacctttg tgaggctgaa gaccgtggcc tttgtatcag
     1741 ctgaattgcg cctggttagc aatggcttca agcaggaggt gagcaatgac ggcttcgtat
     1801 aggcgtttca tggtgtgtgg cttgagtgtg ctggcatggt gttggtgtat cgccggcggt
     1861 acagctgcgc atgagcccgt ggcgacgtct cagtccggca attcagactc ggtacagcac
     1921 cggagcgata tcgcctggga cgcgatcgag tggactgagc ggcgctataa gattgaggcg
     1981 ctgggcttca aggcacggga cgaaaccggc aacagttggt tgggcagcga cgaggtgatg
     2041 atccgtaccg atgatgccaa aggctggacg gtctcgggcc agattggcga catcgactcg
     2101 ggcgacgcgc accactttga tcccgcaaag agttgcatcg cccccgtacg tcccggtatc
     2161 gtcgtgcttg gaaagacatc ggtctgtgac gacagcggcg aagccgcacc gctgagtttc
     2221 gcggtcgagt tctgggaaaa ggattggttc ggcggatttg gccgtgattt ttgcagtcct
     2281 ggctcgccgg cgtcaggcac gcatgcagga ccgcattgtg cgaacgacaa tgccggtgac
     2341 gatttcatcg gtcgtgctca tatcgatctg tcgatacagg accttgaaac agccctgctc
     2401 aacgtcggcg atgaatatat tgaaaccgtc gtactggagc agtgtggcag tggaagtaca
     2461 tgcgacgtga gctatgggcc ggactacagc tttacctatc gcatcacacg tctgcgcgac
     2521 gcgcgggtcg gattgcgccc gatgctcgat aaggcaatgg gcacaatcgg tacgcattct
     2581 gaactcgatg ccatcgctgc cggtctacgg tctttgcgcg cgccaagtcc gcgtcaaatc
     2641 gaacccggaa tgggcaaata gggtggcaag agccgagcgg ccgtcaccct ataagcagac
     2701 tgcagatccg accatgatgt gtcattgccc atcaagagag ttcactcctc atgtggatac
     2761 agttaaacag tagtagccgt cccttgaaat acgacgttca ttggagcgtt cttcggaaca
     2821 cgctgagtat tctccttctc gctttgctcg cgggctgcgg aacgatgagc gggttgtcct
     2881 gcggggccga tgggcagctt gcagtgcaag aattgctgta tttcggaaca gagacgccat
     2941 ctggtcacgt cacacctgaa gcctggacgc aatttctcag cgaaatagtc ac
//
LOCUS       DASTWE010000637         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1471690, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000637 DASTWE010000000
VERSION     DASTWE010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471690"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..952)
                     /gene="folD"
                     /locus_tag="VFT30_06645"
     CDS             complement(89..952)
                     /gene="folD"
                     /locus_tag="VFT30_06645"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU5092345.1"
                     /translation="MAAQLIDGKALAQKVRDRLAKESAELFAKQSMKPGLATILVGDD
                     PASHVYVRNKQKACELAGIYVDDHKLPASTTQAELLALIDKKNTDPKIHGILVQLPLP
                     KHIDSKVILEAVSPLKDADGFHPYNFGRLVEGHPVFEACTPKGVIKMIESTGVTIEGK
                     RAVVVGRSNIVGKPLALMLLQRNATVTICHSKTRDLPAVCREAELLLVAIGKAKFVTA
                     DMVREDAVVIDVGMNKTPEGKLCGDVDFDTVSQKAGWISPVPGGVGPMTIAMLLENTV
                     ESAKRAAGMIL"
     gene            1133..2116
                     /locus_tag="VFT30_06650"
     CDS             1133..2116
                     /locus_tag="VFT30_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092346.1"
                     /translation="MFVKFYHHLILLTVLILGLTFRGEAAQITGLESPNSFVGDPSGK
                     EYFISNINGEPEARDNNGFITKLNAEGKVTDLKFIQGGVADVLLHAPKGMALVGTILY
                     IADLDRLKAFDKTSGKPIATVSFTTASHTGVSLTDVAAAPGGMLYVSDQAANSIYRID
                     PSANHRVDLLIHDERLAGPAGIAVHPRTNHLITVSQHQGKILEITPDGQLTELESNGF
                     FTGRFQNLSGVDFDQWGNMYVSDITKGKIWRMTRDHRFQVIAEYLPAPADIGIDRVNN
                     LILVPYHYLHAAEMNGLETPTSAKPKGEKRTLADYGFIPPPSKSEPEGTKK"
     gene            2350..>2577
                     /locus_tag="VFT30_06655"
     CDS             2350..>2577
                     /locus_tag="VFT30_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092347.1"
                     /translation="MALFNLIEVITFGYFEGRRDGQDVEVVTALQTLRRSLSPLHVPD
                     GLAPVFAEHLKKEYEAFRKQNPEQIADASDAL"
BASE COUNT          599 a          743 c          633 g          602 t
ORIGIN      
        1 gggagggtta tactcaaccg tgactgcaaa cgttcctcgg tcgaggatat cgattaggcg
       61 ctgtggctct cgactcatag atggcccctc ataatatcat tcctgctgcc ctcttggcag
      121 actccacggt attttccagc agcatcgcaa tagtcatcgg accgactcca ccggggacag
      181 ggctgatcca gccggccttt tggctgaccg tgtcaaagtc tacgtcgccg catagtttgc
      241 cttcaggagt cttgttcatc cccacgtcga tgacgaccgc atcttcgcgc accatgtcag
      301 ccgtcacaaa ctttgctttt ccgatcgcga cgagaagcaa ctccgcctca cggcaaaccg
      361 cgggaagatc tctggttttt gaatgacaga ttgtgaccgt cgcattcctc tgaagaagca
      421 tgagcgccaa aggcttcccg acaatattgc tccgtcccac cacaactgcc cgcttgcctt
      481 ctattgtcac cccggtcgac tcgatcatct tgataacacc cttgggggta caagcttcga
      541 agacggggtg cccttctacc agtcggccga agttgtaggg atgaaaccca tctgcgtctt
      601 tgagcggcga aacggcctcc aagatgacct tgctgtcgat gtgtttgggc agcgggagct
      661 gaaccagaat gccatggatt ttggggtcag tattcttttt gtcgatcagt gccaataact
      721 cggcctgcgt cgtgctcgcc gggagtttgt gatcatcaac ataaattccc gccaattcgc
      781 aggccttttg cttgtttcga acatacacat gcgaggcagg atcgtccccc accaaaatag
      841 tcgcaagacc cggcttcatc gattgcttgg cgaacagttc cgccgattct tttgccagac
      901 gatcacgtac tttttgtgcg agcgcttttc catcaattaa ttgtgcggcc acgattcccc
      961 tcccctatga ctatttcggc ccagtaaacc gtcgcaactt agcaggggaa ttttatgcaa
     1021 gtcaacgttt cgccagttct ccctccttgc ctccagcctc agggctcttt cttgacaccc
     1081 tctttgctgc tccggtacga tgaccttcag caaaaccttg gacgacccgc ctttgttcgt
     1141 aaagttttat caccacttaa tactgctcac cgtactcatt ctaggcttga cattccgcgg
     1201 tgaagccgcg caaatcaccg gtttggaatc acccaatagc ttcgtcggcg acccgtctgg
     1261 aaaggaatat ttcatctcga acatcaacgg cgagccggaa gcaagagaca acaacggctt
     1321 catcacaaag ttgaatgccg aaggaaaggt caccgacctc aagttcatcc aaggcggtgt
     1381 ggcggacgtg ttgttgcatg caccgaaagg gatggccttg gtaggcacaa tactttacat
     1441 cgcagatctc gatcgactga aagccttcga taagacgtca ggtaaaccca ttgccacggt
     1501 ctcgttcaca acagcatctc atacgggggt atccctgacc gacgtggcag ccgcgccggg
     1561 cggtatgttg tacgtttcag accaggcggc gaactccatt taccggattg atccgtcagc
     1621 caatcaccga gtcgatcttt tgatccatga tgaacggctc gcaggacccg cgggaatcgc
     1681 ggttcatccc aggacgaatc atctcatcac cgtgagccag caccaaggga agattcttga
     1741 aataactccc gacgggcaac tgaccgagct ggaatcaaat gggtttttta ccggtcgctt
     1801 ccaaaacctg agcggagtag atttcgatca atggggtaac atgtacgtat cggatatcac
     1861 aaaaggcaag atttggcgca tgacccgcga ccatcgattc caagtcatcg ccgagtatct
     1921 tcccgctccg gctgacatcg ggatcgatcg tgtgaacaac ctcatattgg ttccctacca
     1981 ctatctgcat gccgccgaga tgaatggact ggagaccccg acatcggcca agccaaaggg
     2041 tgagaaacgc acgctggccg actacggatt cattcctccc ccttccaagt cagagccgga
     2101 aggaaccaag aaatgagctc atgttcttat tgtcaccagc gaaagggcaa acgatcgtgc
     2161 ccggctctct gaggagtgat ctgtagtctc tgctgtgggg agcatcgcct gacgcggatc
     2221 tcatgtccgt ctgattgcac ctatctcgac accgggagcg attaccaaca aaaaagactc
     2281 ggcccgcaat ttgcgtccgt tcgaagagac ttctatcagg agctggacca attaggaggc
     2341 cacaaagccg tggccctctt caatttgatc gaagtcatca cgttcggcta ttttgaagga
     2401 cgaagggatg gacaggatgt cgaagtcgtt acggctctcc agacgctccg tcgctcgctc
     2461 agcccactcc acgtccccga cggccttgca ccggtttttg ccgaacacct caaaaaagag
     2521 tacgaggcgt ttcggaaaca gaatcccgaa caaattgccg atgcctccga tgcattg
//
LOCUS       DASTWE010000638         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1051588, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000638 DASTWE010000000
VERSION     DASTWE010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051588"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="VFT30_06660"
     CDS             complement(<1..>1147)
                     /locus_tag="VFT30_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5092348.1"
                     /translation="LGYRPGVSKEVFACLEDEQVNVYRDLCNQLVMPDVAKEQPDVVG
                     ISIGTQMQLLAGLTFGKMIKETFPQIHVVVGGNVITRLQEDLMNHEVFFTEIFDSAIL
                     YEGEHALLWLIEALKGQRPLASVPNLIYRDESGLHRNPEIYTEKTTALPLPDFDGMPL
                     DRYFVPELIIPYLATRGCYWGRCTFCDHGQGYFDQYRGMPAQLVIDQIKALRDKYHCR
                     HFLFSDESYPPALFKKVSQLLVDQDVGIKWTTLIRFEETLQDQATWDLAAKAGCCTLY
                     YGMESANERVLNLMDKHATKRVIQRNLQMAAKAGIWNHVMAFYGFPGETFEEAMETRG
                     FVLENQPVIHSLELFYFVAYRHTPMVRHPEKFGITIHKQEEYDLPLD"
BASE COUNT          271 a          328 c          287 g          261 t
ORIGIN      
        1 tagtcaagcg ggagatcgta ttcttcctgc ttatggatcg tgatgccgaa cttttccggg
       61 tggcgcacca tcggagtgtg gcgataggct acgaagtaga acagttccag tgaatgaatc
      121 accggctggt tctccaggac aaagccgcgg gtttccatcg cttcctcaaa cgtttcgccc
      181 ggaaacccat agaaggccat cacatggttc caaattcccg ccttcgccgc catctggaga
      241 ttgcgctgga tcacacgctt cgtggcgtgc ttgtccatga ggttcaagac ccgttcattc
      301 gccgattcca tcccataata gagtgtgcag cacccggcct tggccgccag atcccaagta
      361 gcctgatctt gtagcgtctc ttcaaatctg atgagcgtcg tccacttgat cccgacgtct
      421 tggtcgacca gcagttggga gactttcttg aataacgccg gtggataaga ctcatcgctg
      481 aacaaaaaat gccggcaatg gtacttgtcc cgtagagcct tgatctggtc gatcaccagc
      541 tgagccggca tcccgcggta ctgatcgaaa tagccctgac catgatcgca gaacgtacag
      601 cgcccccagt agcagccgcg cgttgccaag taaggaatga tgagttcagg gacgaagtag
      661 cgatcgagcg gcatcccatc aaaatcaggc aacggcagcg cggttgtttt ctcagtgtag
      721 atttccggat tccggtggag ccccgactca tcgcgataga tcaagttggg aacggaggcg
      781 agaggccgct gacccttcaa cgcttcgatg agccacaaca gcgcatgttc tccttcatag
      841 agaatcgctg aatcaaatat ttccgtaaag aaaacctcat gattcatgag gtcttcctgt
      901 aaccgcgtga tcacgttgcc gcctactacc acgtggatct gcgggaacgt ctccttgatc
      961 atcttgccaa acgttaagcc ggccaagagc tgcatctgtg tcccaatgga aatcccgacg
     1021 acgtccggct gttctttcgc cacatccggc atgaccaact gattgcagag atcccgatac
     1081 acgttcacct gctcatcttc caaacaggca aacacttctt tcgaaacacc gggacgatac
     1141 ccgagat
//
LOCUS       DASTWE010000639         1072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1180676, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000639 DASTWE010000000
VERSION     DASTWE010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180676"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFT30_06665"
     CDS             <1..722
                     /locus_tag="VFT30_06665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5092349.1"
                     /translation="CLPDESEILAELGYLKRVGLQYQWLNHGYQTFDDYLAHFRAKRR
                     NQIKREMREVDDQGVRIETLYGEEIPTEMFPQMFALYKSHIDKLYWGRQYLQGQFFNL
                     LGQRFKRNLCFVVAHYQGRIIAGTFNVQKNGVFYGRYWGAFQELRHLHFNVCYYAAID
                     HCIKQGFVRFEPGAGGDFKRLRGFDPQPTVSMHFLAEPRLAAAVSRFLDNEREQVHRT
                     IGYLQEESELKLNTGEDKEET"
     gene            747..>1072
                     /locus_tag="VFT30_06670"
     CDS             747..>1072
                     /locus_tag="VFT30_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092350.1"
                     /translation="MVPHTLTFLIHNPQFAMDNRQSAVRHQFPIDNPQSASPLRRGAI
                     RQPPPSGGNPQSLGLLSATMLVVGNTIGVGIFTTSGIVADAVPSPGWLLAVWILGGLL
                     SLAGALT"
BASE COUNT          241 a          292 c          285 g          254 t
ORIGIN      
        1 tctgtcttcc tgatgagagt gaaattctcg cggagttggg ctacctcaag cgtgtcggac
       61 ttcaatacca gtggctgaat cacggctatc agaccttcga cgactacctc gcgcatttcc
      121 gtgcgaaacg gcgcaatcag atcaaacgtg agatgcgtga agttgacgat caaggagtgc
      181 ggatcgagac cctctacggc gaggaaattc ctaccgagat gtttccgcag atgttcgcgt
      241 tgtacaaaag tcatatcgac aaattgtatt gggggcgaca gtatctacaa ggacaatttt
      301 ttaacctctt aggccagcgg tttaagcgca atttatgctt tgtcgttgcc cattaccaag
      361 gaaggattat tgccgggacg tttaacgtac agaagaacgg cgtgttctat ggtcgttatt
      421 ggggggcgtt tcaggaactc cgtcatctac actttaatgt ctgctattac gccgcgattg
      481 accactgcat caagcaaggg ttcgtacgct ttgaacccgg ggctgggggc gatttcaagc
      541 gcctgcgcgg gttcgaccca caaccaaccg tgagtatgca tttccttgcc gaaccacgtc
      601 tcgccgccgc agtttctcgt tttctcgaca atgaacggga gcaggtgcat cggacgattg
      661 gctatctaca agaagaaagc gaattgaagc tgaacacggg agaggataag gaagaaacgt
      721 gagacgcggg acgtgatgcg tattacgtgg tacctcatac cttaaccttc ctcatccaca
      781 atccccaatt cgcaatggac aatcgccaat ccgcagtccg tcatcaattc ccaatcgata
      841 atccgcaatc cgccagcccc ctccgtcggg gggcaatccg ccagccccct ccgtcggggg
      901 gcaacccgca atcgcttggc cttctcagtg ccaccatgtt ggtggtgggg aataccatcg
      961 gcgtcgggat cttcacaacc agtggcatcg tggccgatgc cgttccgagt ccgggttggt
     1021 tactcgccgt ctggatcctc ggcggccttt tgtcgcttgc cggtgcactc ac
//
LOCUS       DASTWE010000640         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1277717, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000640 DASTWE010000000
VERSION     DASTWE010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277717"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..671)
                     /locus_tag="VFT30_06675"
     CDS             complement(3..671)
                     /locus_tag="VFT30_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092351.1"
                     /translation="MIPKQPADLIENDKIYFSCEIARIAMRAYLEPDLEAPPRKGITT
                     IVLSVESLHGTMNEIIHDANIFVKGNRDLNIPVHDGIIHYVNVLHELGDNVSLETKIL
                     LASELVGGSSYVRGKNPFNDLDFLIRIRSALIHARPDGFVYTDNVALSTNHDRLIRLL
                     LERHLIDDGQNPFTSVVANKRISQWAFNTVSAMARSIVEIFSSAGMRATLEQRYLETY
                     TFLE"
     gene            838..>1023
                     /locus_tag="VFT30_06680"
     CDS             838..>1023
                     /locus_tag="VFT30_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092352.1"
                     /translation="MARNHQWSSLTPSTAAQVRSTEFKSGMRVLSNSELRGGATHFCS
                     PAGPGIVAGSKGPPMLLT"
BASE COUNT          232 a          275 c          248 g          268 t
ORIGIN      
        1 gtctattcaa gaaatgtgta ggtttccaag taccgctgct ctagtgtggc tctcatgcca
       61 gcacttgaaa aaatctcaac gatcgatcga gccatggcac tgactgtatt gaatgcccac
      121 tgagaaattc gtttatttgc aactactgac gtaaacgggt tctgtccatc atcgatcaag
      181 tgccgttcca acaatagtct gatcagccta tcatgattgg tgctcagtgc tacattatcg
      241 gtatacacga aaccatcagg acgtgcatgg atcagcgcac tccttatccg gatcaggaag
      301 tcgagatcgt taaatgggtt cttacccctc acgtatgatg acccacctac gagctcacta
      361 gcgagtaaga tttttgtttc caatgacaca ttatctccga gttcgtgcag cacatttacg
      421 tagtgtatta taccatcatg tacagggata ttcagatctc gattcccttt cacgaatata
      481 ttagcatcat gaatgatctc gttcatagtt ccatgaagag attctactga cagtacgata
      541 gtcgttatgc ctttgcgtgg cggagcttcc agatccggtt ctagataagc tcgcatcgca
      601 attcttgcta tctcgcaaga aaagtaaatc ttgtcgttct ctatcaggtc ggctggctgc
      661 ttagggatca tgtactccag cccgattcgg aaagcagagt cgcgttgaac cggcctgccc
      721 ctctcgccac ttctcgcttg aggcccgctc gtactcgcca atccagatgc gctggtgggt
      781 tcctcgaccc gcaagccggg acctcgatcg tccgcacctg gagtggctgg gcacaccatg
      841 gcccgcaatc accaatggtc ttcgctcacg ccgtcgaccg ctgctcaggt caggtcgacc
      901 gagtttaaga gtggcatgcg ggtgttgtca aactccgagc ttcgcggggg tgcgactcac
      961 ttctgtagtc ctgctggacc gggtatcgtc gcgggcagca aagggccgcc gatgctgctg
     1021 aca
//
LOCUS       DASTWE010000641         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_502219, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000641 DASTWE010000000
VERSION     DASTWE010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502219"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFT30_06685"
     CDS             complement(<1..190)
                     /locus_tag="VFT30_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEU5092353.1"
                     /translation="MEVRLQKLIASTGLSSRRKAEMLIASGRVSVNGKIVTELGTKVD
                     PARDHVKVDGKHLTSAQPF"
     gene            complement(273..>1819)
                     /gene="guaA"
                     /locus_tag="VFT30_06690"
     CDS             complement(273..>1819)
                     /gene="guaA"
                     /locus_tag="VFT30_06690"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5092354.1"
                     /translation="HDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVPLATILAYR
                     PQGIILSGGPSSVYEKKAPVVAKELFDQNIPILGICYGMQLVTHLSGGTVAKSTHREY
                     GRADLTIDDAGDLFKGVGTDRSTAVWMSHGDRIERMPPGFRSIAHTNNSPVAAMKRDD
                     HKRRIYCLQFHPEVVHTPEGTKILRNFVYEICSCKPTWTMQSYVETAVGQIREQVGKE
                     RVICALSGGVDSSVAAALTHRAIGGQLTCIFVDNGVLRAGERDQVQKTFASQLHLNLR
                     ILDGTKQFLAGLKSVTDPEQKRKIIGRQFIKHFEAESKKLKGIKYLVQGTLYPDVIES
                     ISFKGPSATIKTHHNVGGLPARMKLTLIEPLRELFKDEVRVLGTELELPDEIIWRQPF
                     PGPGLAIRVLGAVTPERLAILRAAEAIVDQEIRSAGLYREIWQAFAVLLPIRTVGVMG
                     DQRTYEHVIAIRAVTSVDGMTADWAKIPNEVLGRMSNRIINEVKGVNRVVYDISSKPP
                     STIEWE"
BASE COUNT          392 a          525 c          475 g          427 t
ORIGIN      
        1 caaatggctg cgccgacgtg aggtgcttcc catcgacctt cacatgatcg cgtgcaggat
       61 caaccttcgt cccgagttcc gtgacgatct tgccgttcac cgacactcgg cccgaggcga
      121 tcagcatctc cgcctttcgg cgggacgaaa gccccgtact ggcgataagt ttttggagac
      181 gaacttccat gtcgctattc gtgaaccgtg aaccgtgaac cgttaatcgc aaggcagctt
      241 cttcctgcgt ttaacgaata acgattaacg tctcattccc attcaatcgt gctgggcggt
      301 ttcgagctga tgtcatacac gacccgattc acacctttga cttcgttgat gatccgatta
      361 gacatgcggc caaggacctc attcggtatc ttcgcccaat cagccgtcat accgtctaca
      421 ctggtaacgg ctcggatcgc gatcacatgc tcataggttc gttgatcgcc catcactccg
      481 acagttcgaa tcggcaagag cacagcaaag gcctgccaga tctcacgata cagacctgca
      541 cttcggattt cctgatcaac gatggcttcc gccgctcgca gtattgccaa cctttccggc
      601 gtcactgcgc ccagcacgcg aatcgccaat ccgggacccg ggaacggttg ccgccagatg
      661 atctcgtcgg gcaattctaa ttctgttccc agcacccgca cttcgtcctt gaataactcg
      721 cgcagcggtt cgataagcgt gagcttcatg cgtgccggta agccgccgac gttatgatgg
      781 gttttgatcg tagccgacgg gcccttgaag ctgatgcttt cgatgacgtc tggataaaga
      841 gtgccctgga cgagatactt gatacctttc agttttttgg attcggcctc gaagtgcttg
      901 atgaactgcc gaccaatgat ttttcgtttt tgttccggat ccgtcacgct ctttagacca
      961 gcgagaaatt gtttggtccc gtccaaaatt cgtaaattga gatgcaactg cgatgcgaaa
     1021 gtcttctgta cttgatcacg ttcaccagcc cgcaggacgc cattgtcgac gaagatgcag
     1081 gtaagctgac cgccgatcgc cctatgcgtc agggccgccg caaccgatga atccacgccg
     1141 ccgctcagcg cacagatgac tcgttccttg ccgacctgtt cacgaatctg cccgaccgcg
     1201 gtctcgacat aggactgcat ggtccaggtc ggcttgcagc tgcaaatttc gtagacgaag
     1261 ttgcggagaa tttttgttcc ttctggcgtg tgaaccactt cgggatggaa ctgcaagcaa
     1321 tagatccggc gcttatgatc gtcccgcttc atcgccgcga caggcgagtt gttcgtatgc
     1381 gcgatcgaac gaaaccccgg cggcatccgt tcgatgcggt caccgtgaga catccacacg
     1441 gcggtcgacc gatcggttcc gactccttta aagagatccc cggcatcgtc gatcgtaaga
     1501 tcggctctgc cgtactcgcg atgtgtcgac ttcgcgaccg tccccccgga gagatgggtc
     1561 accaactgca tgccatagca aataccgagg atcggaatgt tttgatcgaa cagctctttg
     1621 gcaacaacgg gtgccttctt ctcatagacg ctggaagggc cgccggaaag gataatgcct
     1681 tgcggtcgat aggcgagaat tgtcgccaac gggactgtac aaggaagaat ttgcgaatag
     1741 acctgggctt cgcgaatgcg gcgggcaatc agttgcgtat actgcgaccc gaagtcgagg
     1801 accagaattc tatcgtgcc
//
LOCUS       DASTWE010000642         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_857569, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000642 DASTWE010000000
VERSION     DASTWE010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857569"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..381
                     /locus_tag="VFT30_06695"
     CDS             40..381
                     /locus_tag="VFT30_06695"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5092355.1"
                     /translation="MSRLDQAFAALSDPTRRGIVVHLARGEASVSDLVGLFSLTQPTI
                     SSHLKVLESAGLISRSRVAQLRPCKLETGQLKAVTDWLEKVQEVWEGNYKRLDALLAE
                     LKQAQKEERKK"
     gene            378..869
                     /locus_tag="VFT30_06700"
     CDS             378..869
                     /locus_tag="VFT30_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5092356.1"
                     /translation="MKPAEVSTPSDREVLVKRSFDAPVNLVWQAYTDPALMRRWLTAM
                     PGWSMPVCEMTTHVGGKYRWRWRDDANGQEFGFTGEMLEVALHSKIVHTQIYDPGDTD
                     FGSMGGEPSIITVTFNETNGITNVATSIKFASKADRDAAISTGMTDGMEMNYKQLDGV
                     LAG"
BASE COUNT          255 a          253 c          325 g          214 t
ORIGIN      
        1 tctattgaca cttatctata tagacgatag tctatgtaga tgagccgact ggaccaagca
       61 tttgccgctc tttccgaccc cactcgacgt ggaatcgtgg tgcatctggc tcgcggcgag
      121 gcatccgtct ccgacttagt cggcctcttc tcgttgacgc aacctacgat ctcgtcgcac
      181 ctgaaggtgc tggaatcggc ggggctgatt tcgcgcagtc gagtggccca gttgcggccc
      241 tgcaagctgg agacggggca attgaaagcg gtcacggatt ggctggagaa agtgcaagag
      301 gtttgggagg gcaactacaa gcggctcgac gcgttactcg ccgagttgaa gcaagcacag
      361 aaagaggagc gaaagaaatg aaacctgctg aagtgagcac tccgtcagat cgagaagtgc
      421 tggtgaaacg gagcttcgat gcgccggtca atctggtgtg gcaggcgtat acggatcccg
      481 cgttgatgcg tcgttggctc acggccatgc ccggatggtc gatgccggtg tgcgagatga
      541 cgactcatgt gggtgggaag tatcgctggc gctggcgaga tgacgcgaac ggccaggagt
      601 ttgggttcac gggagagatg ctggaggtcg cgttgcattc gaagatcgtg catacgcaga
      661 tctacgaccc gggcgacacc gactttggat cgatgggagg tgagccgtcg atcatcacag
      721 tcacgttcaa cgagaccaac gggatcacga acgtcgcgac ctcgatcaag tttgcgtcga
      781 aggcggaccg tgatgcggcg atatcgacgg gcatgactga tggaatggag atgaactaca
      841 agcaactcga cggagtactg gcgggttaga ttgctgcatt aatttttata tggacgtaca
      901 agaacaaatc cgggagtacc tcaccaacca acctgaacca aaacgtagcg acatgcagtc
      961 gctacacgac atcattctgc gagtaaggcc gtcaagtaaa ttatggttcc tcgatggaaa
     1021 agacagcgag gccaagatcg tttcgaa
//
LOCUS       DASTWE010000643         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_469935, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000643 DASTWE010000000
VERSION     DASTWE010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469935"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFT30_06705"
     CDS             <1..325
                     /locus_tag="VFT30_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092357.1"
                     /translation="VPLRKVIDRREVEQAMSDLPKLLSQARAAPHMVNGTINGFRLDY
                     IAPTSFYEKIGVQAGDVLQRVNGVDIRDPSTMLNLLQQLKNEQIVKLDVLRNNQRSTI
                     TYELR"
     gene            910..>2355
                     /locus_tag="VFT30_06710"
     CDS             910..>2355
                     /locus_tag="VFT30_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5092358.1"
                     /translation="MRGQGNLSERLDNDQSNPSVHRPLLGRILGEKFNLLDSKLQEAL
                     AYQQEKGGLLGEVLLHLRLLREEQLLEALAQQFEMSWMPHLDTTPVDHELIKKVPIAF
                     CRRYRVLPLRYEEGAILTASTDPLETVALDDLRLLLGKPIKPVLTTSIVLLACLNRAY
                     DEIANPAGAEQVMEDIAANQSLDQLAHELDEPQDLLDATDEAPIIRLINSVLFQAVRQ
                     RASDIHFESFERGLVVRYRIDGVLYPVLTPPKHLQASIIARLKIMSSLNIAEKRLPQD
                     GRFAIRTSGKDVDLRVSVLPTSHGERVVLRLLEKENRLLNLSEMGFSKERLAVIHQLI
                     QLAHGIILVTGPTGSGKTTTLYAALSHINAPDKNIITVEDPVEYQLPGINQTQIQDKI
                     KLTFAATLRSILRQDPDVILVGEIRDQETARIALQAAQTGHLVLSTLHTDDAPSTVTR
                     LLDIGVESYVIASATIGVVAQRLVRRLCQSCR"
BASE COUNT          549 a          688 c          616 g          502 t
ORIGIN      
        1 cgttcctcta cgaaaagtca tcgaccgccg tgaggtggag caggccatga gcgatcttcc
       61 gaaactttta tctcaggcca gggccgctcc gcatatggtg aacggaacca tcaacggatt
      121 tcgcttggac tatatcgccc caaccagttt ctacgaaaaa atcggagtcc aggcgggaga
      181 tgtgttgcag cgcgtcaatg gagtcgacat tcgcgatccc agtactatgt tgaatttact
      241 gcaacaactc aagaatgagc aaatcgtcaa gctcgacgta ctcaggaaca atcagcgttc
      301 gacgatcacg tacgaacttc gctgagcatg attggcacac atacgatcct acgatctcct
      361 cgccggttca ccatgctcga tctgagtgtt catggaccct tcaccgaccc atccaaccca
      421 cttgcttgct ccaaggatgg aacaagcagg ccgttgaaaa agtcggtcag cgcgttctcg
      481 cgtcgcttag agggttcaac gtacggcctg ggagaacgcc tgttccgaac aggcgttgga
      541 tgggcaggat aaaattaggt tacgcttcac ctcaagatac caccggctta ccatatcgcc
      601 ggcgcgcaca gaaatgtggc gtcgcatatt cgtgacgcca tgcgccttac cggacgcatt
      661 tttaaccaac ctgctggcta ttgtgaaagg gcctgtggct tctaaatctc ctgctttgcc
      721 acatttacaa aatagcttgg caacaacctg ctagagctcc atctggaacg cttcgatcga
      781 acacatagac gcctccattt gggggaagaa ttcgaggtgt ccttgccagg tcgtcggaag
      841 ggagagtatc tttacgaccg agatggaagc ctatcggaga aacgactatg atggcgatct
      901 tctcctttca tgagaggtca gggcaacttg tccgaacgac tcgacaacga tcagtccaac
      961 cctagtgttc accgtcctct gttgggacgc attctcgggg aaaagttcaa cctcttggac
     1021 tcaaaactcc aggaggcctt agcctatcag caggaaaaag gcggcctgtt gggcgaggtc
     1081 ctgctgcatt tgcgcttgct gcgggaagaa caattgctgg aggcgctcgc tcaacaattt
     1141 gagatgtcct ggatgcctca tctcgacacc acccctgttg accacgagct catcaagaaa
     1201 gtacctatcg cattttgtcg acgataccgt gtcttgccgc ttcgatatga agagggggcc
     1261 attctgaccg catccaccga tcctctggaa accgtcgcgc tcgacgatct tcgattactc
     1321 ttgggtaaac cgatcaaacc ggtcttaacc accagtatcg tgctgcttgc ctgcttgaat
     1381 cgagcctacg acgagatcgc caacccggcc ggcgcggaac aagtgatgga agacatcgca
     1441 gccaatcaga gtctcgacca gcttgcgcat gaacttgatg agccacaaga cttgctggat
     1501 gctaccgatg aggccccgat cattcgattg atcaactcgg tgctgttcca ggctgttcgg
     1561 caacgggcaa gtgacatcca tttcgaatca ttcgagcgag ggttggtggt gagatatcgc
     1621 attgacggtg tactctaccc cgtcctcacg ccacccaagc atttgcaagc cagcattatc
     1681 gcgcgcctca agattatgtc cagcctcaac attgctgaga agcgcttacc acaagacggc
     1741 cgattcgcca tccggacctc cggaaaagac gtcgatcttc gggtctccgt gttacccaca
     1801 tcccacgggg agcgagtcgt gctgcgattg ctggagaagg aaaatcgcct cctgaatctc
     1861 tccgaaatgg gcttctcgaa agaacggctc gccgtgatcc atcagctaat ccagctcgct
     1921 catgggatta ttctcgtcac tggcccgacg ggaagcggca aaaccacgac cctctatgcc
     1981 gccttgagcc acatcaatgc gccggacaag aatatcatca cggtcgaaga tccggtggaa
     2041 taccagctgc cgggcatcaa ccagacgcag atccaggaca agatcaagct gacgttcgcg
     2101 gcaacgctgc gatcgatcct gcgccaggac cccgacgtca tcctcgtcgg cgagatccgc
     2161 gatcaggaga cggcgcgaat cgcgctgcag gctgcgcaga ccggccacct ggtgctctcg
     2221 acgctgcaca ccgacgatgc gccgtcgacc gtgacgcggt tgctcgacat cggcgtcgag
     2281 tcctacgtca tcgcctcagc gacgatcggc gtcgtcgcac agcgtctggt acggcggctg
     2341 tgccagtcgt gccgg
//
LOCUS       DASTWE010000644         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1277810, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000644 DASTWE010000000
VERSION     DASTWE010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277810"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1174)
                     /locus_tag="VFT30_06715"
     CDS             complement(<1..>1174)
                     /locus_tag="VFT30_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU5092359.1"
                     /translation="TVVLNGKGFGTFKSTQFNRVTVNGVSALVQRWEREIIEIKVPFR
                     ATSGPVEVLMGKKKLSAGPLIIMAPRIAAITPTEAERGTTLEITGHYFGVSAGARDPN
                     TMFGVNDVVVGGVGVRPKRWKDDKIELEIPANAVSGDVVVRLASSDPLPDGSCCAPVE
                     YVVSNAVPLKLIPSIRVDPVSGPVGTKVVLFGQGFGTKELMDDAVLIGGRPVTIAQWK
                     DDVIVFHVPLDAESGPLVLKHQGRERVLAQFTVHVPRVISMSPASAPIGTLLRINGEH
                     FGFYSESGSTPYNFTDFNTGENRVEIGGVPAVIYRWNNDRIDVWVPFSAKTGKVVIYR
                     SANTPNIGGLCCAERGTQAIEAGDFTLVTPVIESYEPRTAGLDATVTIKGRGFGTFL"
BASE COUNT          245 a          372 c          304 g          253 t
ORIGIN      
        1 gagaaatgta ccgaaccctc ggcccttgat cgtcactgtg gcatccaatc cagcggtccg
       61 aggttcatac gactcgatga ccggcgtgac gagcgtgaag tcccccgcct cgatggcttg
      121 ggtccctcgc tcggcacaac agagtcctcc tatgttcggc gtgttggcac tccggtaaat
      181 aacaaccttg ccggttttcg cgctgaacgg cacccagacg tcgattcggt cgttgttcca
      241 gcgatagatc accgccggca ctccgccgat ttcgacgcga ttctcacccg tattaaaatc
      301 cgtgaagttg tagggagtcg acccgctctc tgaataaaat ccgaagtgct cgccgttaat
      361 gcgcagcaac gtcccgatgg gcgcgctggc gggactcatg gagatgacgc gaggaacatg
      421 gaccgtaaac tgtgcgagca cccgttcccg cccttgatgt ttcagcacaa gcggacccga
      481 ttccgcgtcg agggggacat ggaagacgat gacgtcatct ttccattgcg cgatggtcac
      541 cggccgccca ccgatcagta cggcatcatc catcagttct ttggtcccga acccctggcc
      601 gaacaagacc actttcgtac cgacaggccc actgaccgga tccactcgaa tggatggaat
      661 cagtttcagc ggaacggcgt tgcttacgac atactcgacc ggcgcacagc atgatccgtc
      721 cggtagtgga tcagaggagg ccaaccgaac cacgacatcg ccgctcacag cgttcgccgg
      781 aatttcaagc tcgatcttgt cgtctttcca gcgcttaggg cgcaccccaa ccccgcctac
      841 taccacatca ttgacaccga acatcgtgtt tggatcacgg gcacctgccg agacgccaaa
      901 gtaatggcca gtgatctcaa gcgtggttcc ccgttccgct tccgtcggcg tgatggctgc
      961 tatccgtgga gccatgatga tcaacggtcc agccgacagc ttcttctttc ccatcaatac
     1021 ttcgaccggg ccgctggtcg ctctgaaggg aaccttgatt tcgatgatct ccctttccca
     1081 tcgctgtacc aaagccgata ccccgttgac ggtcaccctg ttgaactggg tggacttaaa
     1141 ggttccaaac cctttcccgt tcaggaccac ggtt
//
LOCUS       DASTWE010000645         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1407503, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000645 DASTWE010000000
VERSION     DASTWE010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407503"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..770)
                     /gene="purC"
                     /locus_tag="VFT30_06720"
     CDS             complement(66..770)
                     /gene="purC"
                     /locus_tag="VFT30_06720"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5092360.1"
                     /translation="MATGELLYEGKAKKIFSTGNPDQVVQYFKDDATAFNAQKRGTIV
                     EKGVINNKVSERLFRLLEQNGVRTHFVERLNDREMLTKRVRIVPIEVVMRNVVAGSLA
                     KRLGLKEGNRIDPAVVEFYYKNDALGDPLVNDDHLRLMNVATPGVLRELRELGHAVNK
                     ILKPFFSERRMQLVDFKLEFGVFHNKLILADEISPDTCRFWDMTTGESMDKDRFRKDL
                     GKIEEAYQEVLKRVCG"
     gene            complement(793..>1099)
                     /locus_tag="VFT30_06725"
     CDS             complement(793..>1099)
                     /locus_tag="VFT30_06725"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU5092361.1"
                     /translation="PVRKSAEWWGLDQEKRATMMQEHTAATLPYLKTVKRKLYHSSGL
                     DDLDFITYFETSTLEDFHNLVLSLEKVQEFRYTRRFGHPTILGTMKSLDEVVELLAQ"
BASE COUNT          240 a          330 c          251 g          278 t
ORIGIN      
        1 cgagcgacgg tgaaacttag tgcggtcgag ttgaggtgct tgagcggaca aggctcaggt
       61 ctccactatc cacacacccg ctttaacacc tcctgatacg cttcctcgat ctttcccaga
      121 tcctttcgaa accgatcttt atccatcgac tcaccggtcg tcatatccca gaaacggcag
      181 gtgtccggag aaatttcgtc cgccaggatc aatttgttat ggaatacgcc gaactcgagc
      241 ttgaaatcga caagctgcat cctccgctca gagaagaacg gtttgaggat tttgttaacc
      301 gcgtgcccca gttcacgtag ctcacgcaac acacccggtg tcgccacatt catcagccgt
      361 aggtgatcgt cattgaccag cgggtctccc agcgcatcgt tcttataata gaattcaaca
      421 accgccggat cgatccggtt cccctctttc agtccgagcc gcttggccaa actacccgcc
      481 acgacgttcc gcatcacgac ttcgatcggc acgattctga ctcgcttcgt gagcatttcc
      541 cggtcattca atcgctccac aaaatgtgtc cgtaccccgt tttgctccaa gagccgaaaa
      601 agccgctccg agactttgtt gttaatgacg cctttttcga cgatagtgcc gcgcttctgt
      661 gcgttgaagg ccgtcgcatc gtccttgaaa tactgcacga cctgatccgg atttcccgtc
      721 gaaaagatct ttttcgcctt accttcgtaa agaagctcgc ccgtcgccat gatcggacct
      781 cagtgattca gcttactgcg ccaacagctc tacaacctca tccaacgatt tcatcgtacc
      841 gagtatggtt ggatgtccaa atcgccgcgt gtagcggaat tcctgcacct tttccagcga
      901 cagtaccagg ttgtgaaagt cctccagcgt ggaggtttca aaatacgtaa tgaagtccag
      961 atcatccagt ccgctggagt gatagagttt ccgtttgacc gtcttcaagt agggcagcgt
     1021 cgcggcggta tgttcctgca tcatcgtggc ccgtttttcc tgatctagtc cccaccactc
     1081 agccgatttc ctgacaggg
//
LOCUS       DASTWE010000646         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1148578, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000646 DASTWE010000000
VERSION     DASTWE010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148578"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFT30_06730"
     CDS             <1..226
                     /locus_tag="VFT30_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEU5092362.1"
                     /translation="WQGPKAGSKRYWSADAMRRSDALDLNGGVFRMNNAREIAIPLKR
                     SSARSKRRKGTPSQSAMSMAELYINRLERS"
     gene            complement(399..950)
                     /locus_tag="VFT30_06735"
     CDS             complement(399..950)
                     /locus_tag="VFT30_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU5092363.1"
                     /translation="MEHSLKDQVGITDEQVRAAIQEGPVTRQYLADPQSIVSSLNRLR
                     STEITSYLQYKQHGYMAVSLLSPGLKTEFESHAQQELAHADRLASRIQQLGGVPIFDL
                     RELAGKAAAVGVHPEQGTTLTEMVVENLLLERRQVEAYTALIREIGDKDLVTREMLLE
                     ILTETETHASELADFLKRSSEKR"
BASE COUNT          229 a          281 c          286 g          253 t
ORIGIN      
        1 gtggcaaggg ccgaaagccg gatcgaaacg gtattggtcg gccgatgcca tgcgacgcag
       61 cgatgcgctc gatctcaacg gcggcgtctt tcggatgaac aatgcgcgcg agatcgccat
      121 cccgctcaaa cgttcttccg cgcggagtaa acgacggaaa ggcacgccct ctcagtccgc
      181 catgtcgatg gctgaacttt acatcaaccg gctggaaaga tcgtaagtcc aaaacagaaa
      241 caaatattcg agcgtgtaag actggcactt cgaaaagtat ttagacggga aaaacgagtc
      301 gcgtagcctc acaaggccat ccacttcgcg gctctgctgg aggatatgtt tgcgttctgc
      361 tgacttgccg aggtcgtggt tgtcgaatgc cttcttcctc agcgcttttc gctggaacgt
      421 ttaaggaaat cggcgagttc gctcgcatgt gtttccgttt cagtgagaat ctccaagagc
      481 atctcacgtg tgacgagatc tttgtctccg atctctcgaa tcagtgccgt ataggcttcg
      541 acctgtcgcc gttcgagcaa aaggttttcg acaaccattt cggtcagcgt cgttccttgt
      601 tccggatgta cgccgacggc ggcggctttc cccgccagtt cccgaagatc gaaaatcggc
      661 acgccgccga gctgctgaat acggctggcc agccggtcgg cgtgtgccag ttcctgctga
      721 gcatgcgatt cgaattccgt cttgagccca ggggacaaca gagacaccgc catgtacccg
      781 tgttgcttgt attggagata gctcgtgatt tccgttgaac ggagccgatt tagcgagcta
      841 acgatactct gcggatcggc gagatactgt ctggttactg gtccctcctg gatggcagca
      901 cgaacttgct catcggtgat gccaacttgg tccttcagcg aatgttccat gacgttctcc
      961 tttcatcgat agggatctca atttcccaaa tccgtgcggt tttgtaaggt aaactagagc
     1021 agctgacgtt attcgaacca atagaaggc
//
LOCUS       DASTWE010000647         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_146798, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000647 DASTWE010000000
VERSION     DASTWE010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146798"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>1234
                     /locus_tag="VFT30_06740"
     CDS             15..>1234
                     /locus_tag="VFT30_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU5092364.1"
                     /translation="MREAQNGGDFVALIMEQMGLRVRVISGTEEARLIFLGVKHSIAL
                     PDGPTLVVDIGGGSVELIVGNREGLIHGKSLKLGAIRLAEQFLPKTPPSESMMHALDD
                     AVLTHLRDALGSFKMKKFHSLVATSGMAGNLGEVVHLRQTGRPLPQLNLATVLLKDIR
                     SLEAELARSSVKARLAISGLDPKRVDTLLPTAVVLRRLLELSGLSEVTLCDKAIREGV
                     IYDFIVRHREGLKAENDIPDVRRRNVIGLARRCQAPEVHSLHVANLALSLFDQTKREH
                     RLGQQERTWLEYAAILHDVGYLINPRQHHKHAYYLIKHSDLGGLAAEEIDVIANIARY
                     HRRALPSLKHEALDCLTPRLQRVVKILASLLRLADGLDRTHFSLVRAVTVKFGKQIAI
                     EVHLTGDAEMELWAA"
BASE COUNT          266 a          337 c          356 g          275 t
ORIGIN      
        1 tcgcaaccag tgccgtgcgc gaagcccaaa acggcggtga tttcgtcgcc ttgatcatgg
       61 agcagatggg gctgagggtg cgggtgatca gcggaaccga ggaagctcgg ctgattttcc
      121 tgggcgtcaa gcacagtatc gcccttccgg acggaccgac gctcgtcgtc gacatcgggg
      181 gaggttccgt ggagttgatc gtggggaatc gagagggcct gatccacggc aagagtctca
      241 aacttggagc aatccgtttg gccgaacaat tccttcccaa aactccaccg tctgagtcga
      301 tgatgcatgc tctggacgac gccgtgctga ctcacctgcg cgatgcgctc ggatcattca
      361 agatgaagaa gtttcattca ctggtggcca cctccggtat ggcagggaac ctcggtgagg
      421 tcgtccatct ccgtcaaact ggacgaccgt tgccccagct taatcttgcg actgtcttac
      481 tcaaagacat ccgtagcctc gaagcggagt tggctcggtc atcggtgaag gctcgtttgg
      541 caatttccgg cctagacccc aagcgtgtcg atacactgtt gccgaccgcc gtggtcctcc
      601 gacgtctttt ggagctgtct ggcctcagcg aggtgacgct ctgcgacaaa gctattcgag
      661 agggtgtaat ctatgatttt attgtacggc accgagaagg cctgaaagcc gaaaatgata
      721 ttcccgacgt tcgccgtcgc aacgtcatcg gccttgctcg acgctgccag gcaccagagg
      781 ttcattcact gcatgtggcc aacttggcac tgagtctgtt cgatcaaacc aagagagaac
      841 atcgtttagg acagcaggag cgaacctggc tcgagtacgc agccattctg cacgatgtag
      901 gatatctgat caatccgaga caacatcata aacatgccta ctacctcatc aaacatagcg
      961 acctgggtgg attggcggct gaggaaatcg atgtgatcgc caacattgcc cgttaccacc
     1021 ggcgggcgtt gccgtcgctg aagcatgaag cactcgactg tctgaccccg cgcttacaac
     1081 gtgtggtcaa gatcctcgct tcgttgcttc ggctagccga cgggctcgat cggactcatt
     1141 tctcgcttgt tcgggccgtg accgtcaagt ttgggaaaca gattgcgatt gaagttcatc
     1201 tgacaggaga cgcggagatg gagttgtggg ccgc
//
LOCUS       DASTWE010000648         1365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_567221, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000648 DASTWE010000000
VERSION     DASTWE010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567221"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..758
                     /locus_tag="VFT30_06745"
                     /pseudo
     CDS             195..758
                     /locus_tag="VFT30_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            complement(840..>1365)
                     /locus_tag="VFT30_06750"
     CDS             complement(840..>1365)
                     /locus_tag="VFT30_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU5092365.1"
                     /translation="WLQQNHGREQEVWLVYLKAGGGRMNIDYESSVEEALCFGWIDSI
                     IQKIDEEKYARKFNPRRTNSKWSETNKRRVIKVIRQGRMTQAGLAKVTFDVNDVDTGK
                     PTPKRTAVQMPENMERALKSRPGLWEAFQKVSPSYRRNYILWLSDAKKPETFARRFQI
                     LFDEVMSGKPTSMH"
BASE COUNT          315 a          354 c          375 g          321 t
ORIGIN      
        1 cggtgttgaa gcggcgcaat aacaggacct gatcttcacg aaaaaacaac aaatgtacgg
       61 taacgggaaa ttttgctcgc atgggttaat tatacttttg tgctccattg taagattaat
      121 acgttttgac tcgccaataa aacatggact tcaaactgac gattttctcc gattacatct
      181 gaccgtggtg ctatgtcggc cagggtgtgg tcgagcggtt gaaagcggaa cataatgtgg
      241 acgtggaatg gcgtccgttc tatctgtatt ttgacacgcc gcccgaggga cgggaattac
      301 cggagcgcgt gaaacaggca cgcgcgcacg gctcggaaga gcgactgcgc cagatcgcgc
      361 aatcctatgg gatgcctttc gtgtccacac cgcggatcta caatacgcgc ctggcccacg
      421 aagcgacgga atatgctcgc gtgcatagca aaggcaatga attccacagg gtcctcttcc
      481 gaaaggtgta tgccgaagga caggacccaa gccaatggga agtgcttcgc gctgcggcag
      541 aggaggtggg gctggataca gacgaaatgc agcgcgaggt ggaggatgaa aaatacatgg
      601 cagaggtcgt agacaaggtc cgctgggcgt accagatcgg cgtgaccggt gtgccaacct
      661 atgtgatcaa cgaccgctac gcgatcgttg gagcacagcc atatgaggta ttcaaggggg
      721 cgctggaaca gatcatgaat caaaagcctg acgagtgatt cgtcagacta tccattgcat
      781 ctcatgcttc gctcagcatt tctcacaccg tcccggtgtc cttcgggaga gtgacttgat
      841 caatgcatcg acgtcggctt cccggacatc acttcatcga ataatatttg gaatcgccgc
      901 gcaaaggtct caggcttttt ggcatcggaa agccagagga tgtaattccg tcgataggat
      961 ggcgatacct tctggaaggc ttcccacaac ccggggcggg atttgagggc cctctccata
     1021 ttctcgggca tttgcacagc agtacgcttc ggcgttggct tgccggtatc cacgtcattg
     1081 acgtcgaacg tgacctttgc cagtcctgct tgggtcatgc gtccttgccg gatcactttg
     1141 atcacgcgcc gcttgttcgt ctcggaccat ttgctgttcg tgcggcgcgg gttgaatttg
     1201 cgggcatact tctcctcatc gatcttctgg atgatgctgt caatccagcc gaagcataag
     1261 gcttcttcga ccgaactttc ataatcaata ttcatcctgc cgcctcccgc tttcaaatag
     1321 acaagccaga cttcctgctc gcggccatga ttctgctgca gccag
//
LOCUS       DASTWE010000649         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1504193, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000649 DASTWE010000000
VERSION     DASTWE010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504193"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..697
                     /locus_tag="VFT30_06755"
     CDS             179..697
                     /locus_tag="VFT30_06755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5092366.1"
                     /translation="MTFPVVSTSEHQGKSLVIDSGREMINVHDTSGQLLGSMSWGAII
                     ERVLVDGEDARFAHCRTQPRAPLALKVRYTTPEGKQFDSLTGGIGGGGLFIESGAPLS
                     PGTELTVEFSLPDRPWDKLKAKAKVAWVRNKPERYLLFPGMGVQFVELDKKVQKQLVG
                     LVDALNRSRNVS"
BASE COUNT          253 a          258 c          275 g          232 t
ORIGIN      
        1 ctgtccgaaa gccgaccaat atacgttcag tcctggcatg gggatacggt tcactgagat
       61 agcggaggat gtccgcgatc gaatccatga gttagtgaaa tcaattcaga acatagatca
      121 agcagcctaa tccgctcctg acttagatta cctagaaaaa gggaaaagga atgatcctat
      181 gacgtttccc gttgtttcca cttccgagca tcaggggaaa tctctcgtga ttgattctgg
      241 gcgagagatg atcaacgtcc atgacacatc gggtcagctt ttaggcagca tgtcatgggg
      301 tgcgatcatc gagcgagtgc ttgtcgacgg ggaagatgcc cgatttgccc attgtcgtac
      361 ccagcctcgt gcgcctctgg cgctgaaggt ccggtacacg acgccagaag gaaagcagtt
      421 cgatagtcta accggtggaa tcggtggcgg cgggctcttt atcgagagtg gtgctcctct
      481 cagtcctggc acagagctga cggtcgaatt ttcgcttccg gatcggccat gggacaagct
      541 gaaagcgaag gcgaaggtcg cctgggtccg caacaagccg gaacgatacc ttttgtttcc
      601 aggcatgggc gttcagttcg tggaattgga taagaaggta cagaagcaac ttgttggact
      661 ggttgatgcc ttgaatcgaa gtcgaaacgt ctcgtgagaa cgagtgaccc tacgaccata
      721 gatccgtccc tcaagaaccg aagctggtgc gctgctttgg ccgaaaccaa aacgactgga
      781 tcttagcccc ttagcagatc ccgcagacat agcagaatct gacggactct cgtttctgag
      841 gcatcgatgg cctattgata aacaatcagt actacccgct cgacagcgcc aaagaaggcc
      901 tcgtacacgt tccatgcatc tatacatcag ctgatattca gtcccccaaa tgccctgttc
      961 aatggtcacg tcaggttccg gtgcctggat gtcttggtaa ctaccgacag cgacatga
//
LOCUS       DASTWE010000650         1487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_17670, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000650 DASTWE010000000
VERSION     DASTWE010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17670"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(785..1177)
                     /locus_tag="VFT30_06760"
     CDS             complement(785..1177)
                     /locus_tag="VFT30_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092367.1"
                     /translation="MADIQSMYHAPSRSEALQSQIEKLEGLAHHTVVNPTFDQFDADT
                     EEMLAKIYGPDHRYVESYKYATVGEAEALVNLPESAQEPLTRDIPKKGLQQRRQALLG
                     MLTELQALEAQEAEAITGEDHEDPPGPS"
BASE COUNT          321 a          395 c          373 g          398 t
ORIGIN      
        1 gcccatgtaa gcgtccactt gtgttgcggt cgggacaaat tcgattgggg agtacggtgt
       61 gttttcaccc tgctttcgac acgattgtcc gacgaggaaa ttcttggccg aacggtttgt
      121 cgacggatca tcttctcggc gtgagatgcc attcaacacg gacttggtcg gtaccatgcg
      181 aggatttgtg aaggactcag tgtcggctca tgcttggata cgccaggacc gatccattga
      241 tcggacctcc gactggatcg cgggcatcaa tcactccatg cgcgacctcc agtgctgccg
      301 cttccgcctc gtcgcgcgta gcgaagctgc ctataggata tcctgattcg ctgaatgagt
      361 atgttggcct gagttctact atggtgtact cacagaccca tgtctcctca tctttccgcg
      421 tggaccataa atggatcgac cgtccattat atctggattc cgtctcaacg tttcctccgt
      481 cggcatggac atcggaaatc tggctcgcct gggtatgcaa atcaagacag gggtctgcgt
      541 gcattgatgc catattgcac ctccctgagt gcggaagctt ccgcctcctt gcgagtttca
      601 acctgtcctt tgtacccacc attctcacgg caggctctcc ataacccttc catccgaata
      661 aaatacatga cagcttcttc gtactataaa tttatgctca tagtgcaact ttgatccgag
      721 aaagtgatgc agggtattat cccctcaaga catgttaagt ctcaaagagc aatgcattgt
      781 catgctagct ggggccggga ggatcctcat gatcttctcc ggtgatcgcc tccgcttctt
      841 gcgcttcaag tgcctggagc tcagtcagca tccctagtag cgcctgccga cgttgttgga
      901 gtcccttctt gggaatgtct cgcgtcaacg gttcctgcgc tgattccggc aaattgacga
      961 gcgcttcggc ctcgccaaca gtggcatatt tgtacgattc cacataccga tgatctgggc
     1021 cgtatatttt ggccagcatc tcctccgtat cagcgtcaaa ctgatcgaag gtgggattta
     1081 cgaccgtgtg gtgcgcgagg ccttcgagct tttcaatctg cgactgtaga gcctcgctcc
     1141 gggatggtgc atggtacatg gactgtatgt cggccataac ctcatctcct ttccctagtc
     1201 agatggaatg gccctgtagg gcacacatag tgaagtataa gatgtccctg acgatacccg
     1261 cctcatcgat gtccactagc tgaatacacc tcttcatgtc gtctttacat actatgaaga
     1321 aactgtttaa gcacgcccat gagaaaatga gaagcctgat ttgccatgga aatctgtgga
     1381 gaaagttccg gctcgcgttg aacgattatg gactcggtga agcgctgggt gaagaggttc
     1441 tcaattggaa ctatggtcat gacaatctcc ttagatcgat gagccta
//
LOCUS       DASTWE010000651         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_793107, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000651 DASTWE010000000
VERSION     DASTWE010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793107"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>1032
                     /locus_tag="VFT30_06765"
     CDS             60..>1032
                     /locus_tag="VFT30_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092368.1"
                     /translation="MSLHIHGSSMVVSATGASAATTNADSIVAKEKSGEFEWYIHPST
                     QEGVRQFHTFANDRELTVMGLFGSFIVEPRGSEYLEPLGTGDATPATSGWQVIIKNGT
                     GPDFREFVLMYHEVGDEAFRPVNKKGDFLPQRDPLTDAYRPGGRAINYRSEPFGINNM
                     HVQHEYFGFEDESMGYSSYTFGDPSPTIPRSYMGDPAKFRLVHGGSEVFHSHHPHGGT
                     IRWPRSPRAIDDMNLWTTAGNGPVKYPVIRAKTDRVDVEVIGPSEALDLETECGSGLC
                     QQLAGDFLFHCHVAHHYVAGMWGYWRVYNTLQQGEMRNDVMPDLRELP"
BASE COUNT          223 a          288 c          310 g          211 t
ORIGIN      
        1 gcaaccaagg agattgcgtc aagatcaagc tgagcaacaa attggaaggt ggcgaggacg
       61 tgagcttgca catccatggc tccagcatgg ttgtcagtgc aaccggcgcc tcggctgcca
      121 cgacgaatgc cgattcgatt gtcgcaaaag agaagagcgg agagtttgag tggtacatcc
      181 atcccagcac gcaggagggg gtgcgacagt tccacacctt cgccaacgat cgtgaattga
      241 cggtgatggg gctcttcgga tccttcatcg tcgagccacg cggatctgaa tatctcgaac
      301 cattaggcac cggtgatgcc acccccgcca ccagtggctg gcaggtcatc atcaagaatg
      361 gaacgggtcc tgatttccgg gaattcgttc tcatgtatca cgaagtcggc gacgaagcct
      421 ttcgccccgt caataagaag ggcgacttcc ttcctcagcg ggatcctctg acagatgctt
      481 atcgtcccgg tggccgcgcg attaactatc gcagcgagcc gttcgggatc aacaacatgc
      541 atgtgcagca cgagtatttt ggattcgaag acgagtcgat ggggtacagc tcctatacct
      601 tcggcgatcc gtcgcccacg atcccgcgtt cctacatggg cgacccagcc aagttccgtc
      661 tggtgcacgg tggatcggaa gtgttccata gccaccatcc gcatggcggc acaatccggt
      721 ggccgcggag cccgcgtgcc atcgatgaca tgaatttatg gacgaccgcc ggaaacgggc
      781 cggtcaagta tcctgtaatc cgtgccaaga cggaccgtgt ggacgtcgaa gtcatcgggc
      841 cgtcagaggc cttggacctc gagacggagt gcggctccgg cttgtgccag caattggccg
      901 gcgacttcct gtttcattgc cacgtggcgc accattatgt ggcaggcatg tggggatatt
      961 ggcgggttta caacacgctg cagcagggtg agatgcgcaa tgacgtcatg ccggacttgc
     1021 gcgaattgcc tg
//
LOCUS       DASTWE010000652         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_49996, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000652 DASTWE010000000
VERSION     DASTWE010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49996"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VFT30_06770"
     CDS             <1..1148
                     /locus_tag="VFT30_06770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5092369.1"
                     /translation="FSLLGHQGPIDVFCPVADTDWFFANALQLGAASKGSMRILDVNA
                     FLTADQMNHYKAVVISTGNSNHCIYIHDVLKKAASLGDLSRFIVYLHDPCILNFVQRG
                     GMLTPSEMVRSFEAIYERKIPITPSDLASSDVVRATLAEQGLMGVRFLLNSGVKRYIV
                     NSRAAFDLVKADLASEAAQVTQLFHPAFLPRGGEDALSIARARVLAGRSEQFTIGTFG
                     VPGPSKLTHRVAAAAREIGRRGVPVRVLMAGYGVREYLRNHGRDFDGLDLRSFDGPTD
                     PQLLRCMAETDVAVQFRSKNLGESSGIVPQLLCLGKTVLVSDIGSFKEFGNAVRMVSN
                     DLDVESLADEILHLWHNPIFQKHTSDYVSRHTPARFREKLIEIVATS"
BASE COUNT          270 a          336 c          366 g          284 t
ORIGIN      
        1 atttttcgct attgggacat caggggccga tcgacgtctt ctgccccgtt gccgataccg
       61 actggttctt tgcgaatgcg ctgcagttgg gggcggcgtc aaaagggagc atgcgcatac
      121 tggacgtgaa cgctttcctt accgcggacc agatgaacca ttacaaagcc gtcgtcatca
      181 gtacgggaaa ttccaatcac tgtatctata ttcacgacgt gctgaagaag gccgcttcat
      241 tgggtgatct cagcaggttt atcgtatacc tgcatgaccc atgcatccta aatttcgttc
      301 aacgaggagg aatgctgacc ccctccgaga tggtcaggag tttcgaggcg atttatgaac
      361 gcaaaattcc catcactccg tcggatctcg cgtcgagcga cgtcgttcga gcgacgctgg
      421 cggagcaagg actgatgggt gtgcgttttc tgcttaacag cggggtcaaa cgatacattg
      481 tcaattctcg ggcagcgttc gatcttgtta aagcggacct tgcgtctgag gcggcgcagg
      541 tgacccagct gtttcatcca gccttcctgc cgagaggtgg cgaagatgct ctttccattg
      601 cgagagcccg cgtgctggcc ggtcggagcg agcaattcac aatcgggact ttcggtgtgc
      661 cgggaccttc aaagctgacc caccgggttg cagctgccgc gcgggagata ggaagaagag
      721 gcgtgccggt gcgagtgctg atggcggggt acggcgtacg cgagtatctg cgcaaccatg
      781 gtcgcgactt cgacggactg gaccttcgct cgtttgacgg cccgacggac ccgcagcttc
      841 tcagatgtat ggccgagacg gatgtcgcgg tgcaattcag gtccaagaat ctcggcgaga
      901 gctccggaat tgtccctcag ctcctgtgtc tcggaaagac ggtcctggtc tcggatatcg
      961 gctccttcaa ggagttcggg aatgcggtga gaatggtttc gaatgacctg gacgtcgaaa
     1021 gcctcgcaga cgagatcctt catctctggc acaatccaat attccagaag cacacatccg
     1081 actacgtttc acgacatacg ccagcgcggt ttcgtgaaaa actaatcgag atagtcgcga
     1141 catcatgact taagctgagc agttgcaaat gactctagtg tcatgaccgg cgattcccct
     1201 tcgatcacgg cgagcgcgtc gtccgacctg attgaacgca tgcgcgctga ctggaa
//
LOCUS       DASTWE010000653         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_276064, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000653 DASTWE010000000
VERSION     DASTWE010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276064"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..167
                     /locus_tag="VFT30_06775"
     CDS             48..167
                     /locus_tag="VFT30_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEU5092370.1"
                     /translation="MDRLESEMKTAAKELAFERAAELRNQIRSLRLQALNTKS"
     gene            complement(164..424)
                     /locus_tag="VFT30_06780"
     CDS             complement(164..424)
                     /locus_tag="VFT30_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HEU5092371.1"
                     /translation="MRKSPWVLIIFVLIGGLLGGILGEILHVMAPQGTIQSIFATHFN
                     PGINPPLTIDLVLIKLVFGFNIKVNILSILGMFIGIYLYKHV"
     gene            653..1999
                     /gene="ffh"
                     /locus_tag="VFT30_06785"
     CDS             653..1999
                     /gene="ffh"
                     /locus_tag="VFT30_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5092372.1"
                     /translation="MLDALSDKFEKILKKLRGQGVLTEQNITEALKDVRFALLEADVN
                     FKIVKEFIERVRQKAVGQEVLQSLTPGHQVVKVVWDELKAMMGHERAGLALSSKPPTI
                     LMMVGLQGAGKTTASGKLARLFKNQGKRVLLVAADPRRPAAGEQLSALGRDLGVEVHR
                     ADHVQASQTDVVRICRAGVERGYDQGFDLIILDTGGRLHIDDELMDELVAVKAAVSPH
                     EILLVADAMTGQDAVTMAGQFDQKVGLTGIILTKVEGDARGGAVLSIRAATGKPIKFL
                     GVGEKLDALEPFHPDRMASRILGMGDVLSLIEKAQESITREQAEEAQKRLTSNTFTLE
                     DFRTQLGQMSRMGSFEQILGMLPGGQKLKQAIEGDKPEREIGRVVAVIDSMTSRERRD
                     HTIINGSRKKRIARGSGTTVQDVNRLIKQFLSAKKLAKAMTGAGGRRQLAQLMRSR"
     gene            2034..2327
                     /gene="rpsP"
                     /locus_tag="VFT30_06790"
     CDS             2034..2327
                     /gene="rpsP"
                     /locus_tag="VFT30_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5092373.1"
                     /translation="MAVHLRLARTGRHKRPMYRVVAADSRKARDGRFLEILGIFDPLK
                     EAGMPELKQERVLTWLRHGAQPTVTVRTLLRRAGVWKQFEAEKAAQKKEPAKS"
BASE COUNT          564 a          546 c          709 g          510 t
ORIGIN      
        1 ccgaatcgat cgaaccatac gggagttcgg aatcaacgga acagctcatc gatcgactgg
       61 agtcggaaat gaaaactgcc gccaaagagc tggcctttga gcgggctgca gagttgcgca
      121 atcaaattcg atcactcagg cttcaagcgt tgaatacaaa gtcctagacg tgtttgtaga
      181 ggtagatccc gatgaacatc ccgaggatgc tcagaatgtt gactttaatg ttgaatccaa
      241 agacgagttt gatcaggaca aggtcgatgg tgagcggcgg gttgatgccg ggattaaagt
      301 gtgtcgcgaa aatactttga atggtgccct gaggtgccat aacatggaga atttcaccaa
      361 gtatcccgcc aaggagccct ccgatcagga cgaagatgat caaaacccag ggcgattttc
      421 tcacgccgcc attcttcttt ctttataagt ttgcgtacag tggctcttgg tggctgaagg
      481 gcaccatatc cgaaggtttt tggcatgtca ataaaactac tgattgtgat gaccttgact
      541 tgatctgctt ggtccgatac actcctcgat gctcttttcc caggattttc gaggcgatcg
      601 gaggtctggt gcaaggcttc gatggcctct ttctttgtgg tgatgtgacg tcatgctcga
      661 tgcgttgagc gataagtttg aaaaaatttt gaagaaactc cgagggcaag gtgtgctcac
      721 ggagcagaat atcaccgaag cactgaaaga tgttcggttt gcgcttcttg aagcagatgt
      781 caactttaag atcgtcaaag agttcatcga gcgggtccgt caaaaggccg tgggccagga
      841 agttctgcag agtctgactc ccggacatca ggtcgttaaa gtcgtctggg atgagctcaa
      901 ggcgatgatg gggcatgagc gcgcaggact ggccttgagc tcaaagccgc cgaccatcct
      961 gatgatggtt ggattgcaag gagccggaaa gaccacggcc agcggaaagc ttgcgcgtct
     1021 gtttaagaat caaggcaagc gagttctttt ggttgccgcc gacccgcgtc gtcctgcggc
     1081 cggcgagcaa ttaagtgccc tcggtcggga tctcggggta gaggttcacc gcgcggatca
     1141 cgttcaggct tctcaaacgg atgtggtacg tatctgccgt gcgggggtag agcgagggta
     1201 cgatcagggt tttgatctca tcatcctgga taccggcggc cgattgcata tcgacgacga
     1261 gttaatggac gaacttgtcg ccgtgaaagc agccgtgtct cctcatgaga tattgttggt
     1321 tgccgacgcg atgaccggcc aggatgcggt caccatggcc ggccagtttg atcaaaaggt
     1381 cgggttgacc ggtattattc tgaccaaggt cgaaggggat gcacgaggcg gcgcggtctt
     1441 gtcgattcgg gctgcaaccg gcaaacccat caagtttctt ggtgtcgggg aaaagctaga
     1501 tgcactggag ccgtttcatc cggatcggat ggcttcccgt attcttggga tgggcgacgt
     1561 gctgtcgctt attgagaaag cccaagaaag catcactcgg gagcaggcgg aggaagctca
     1621 gaagcggctt accagcaata ccttcaccct ggaagatttt cggactcagc tcggccagat
     1681 gagccgcatg ggctcgttcg agcagattct tggcatgctt cctggggggc aaaaactcaa
     1741 gcaagcgatc gaaggcgaca agccggagcg agagatcggc cgtgtggtcg cggtgatcga
     1801 ttcgatgaca tcgcgggaac gccgcgatca tacgatcatc aatggaagcc ggaagaagcg
     1861 gatcgcccgt ggcagcggca cgaccgtgca ggatgtgaat cgtctcatca agcagttctt
     1921 gtccgcgaag aaattggcaa aggcgatgac cggtgcagga gggcgtcgac agctcgccca
     1981 actcatgcga tcgcgatgac agacaatgcg aatacagcat aaaggaggaa gttgtggctg
     2041 tacacttaag actggctcga acaggaagac acaagcgacc gatgtatcgg gtggtagccg
     2101 ccgattcacg gaaagcacgt gacggacggt tcttggagat ccttggaatt tttgacccct
     2161 tgaaagaagc cggcatgccg gagttgaagc aggaacgtgt tctgacctgg ctgagacatg
     2221 gcgctcagcc tacggtgact gtacggacgt tgctgcgccg ggcaggagtc tggaagcagt
     2281 ttgaggctga aaaagccgcg cagaaaaaag aacctgcgaa atcctgatc
//
LOCUS       DASTWE010000654         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1278100, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000654 DASTWE010000000
VERSION     DASTWE010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278100"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFT30_06795"
     CDS             complement(<1..303)
                     /locus_tag="VFT30_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092374.1"
                     /translation="MNRLFKGLYALMGVLVLAGCSSYHHHGMMSGGKSEAYWQKGQQD
                     MEGLINRTVKDPDKAKQVNVLVGEIIKELKASREQEQAYHRDIYTLNTSYTAAPEEF"
     gene            365..709
                     /locus_tag="VFT30_06800"
     CDS             365..709
                     /locus_tag="VFT30_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092375.1"
                     /translation="MPEAPPMTPDRLSALKVEFRHHLETFYAGLQLAPPYESVEKAIR
                     TLTTIIHGLPIEEQTRIMADPRLRWQQFRLAFDASGLAKKHRGIIAGLARNRSDLNLP
                     AEYDQFLNLFLA"
     gene            complement(899..1546)
                     /locus_tag="VFT30_06805"
     CDS             complement(899..1546)
                     /locus_tag="VFT30_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092376.1"
                     /translation="MPRSYVIATFMILAGFFAGILCIGLIVGDWLYFIRLTPDAIRYG
                     CRVAKNQDQWPGTMLTTLGDRFTREGVLMLPHGVAWFYPQLSQIAIRPQYRLFSRRFR
                     TAWPVKGLIHLSPHDQSLNTLCVKRMPWSSALMTLLWFALVLIGSLTFVVQYAMDGGF
                     ASLGGTLTGVAVIALAALVLAFGLVTVALAYRLEDSRLMKVYGELRDAVALPISS"
BASE COUNT          453 a          531 c          507 g          425 t
ORIGIN      
        1 aaattcttcg ggggccgcgg tgtagctggt gttcaatgtg tagatgtctc ggtgataggc
       61 ctgttcttgt tcgcggcttg ccttgagctc cttgatgatc tcgccgacaa gcacattgac
      121 ctgtttcgcc ttgtcgggat ctttgactgt tcgattgatc agtccttcca tgtcttgctg
      181 gcccttctgc cagtaagcct cgctcttgcc accactcatc atgccgtgat gatgatatga
      241 agagcaccca gccagtacga gaactcccat cagcgcatac agccccttga acagccgatt
      301 catacgttcc tccgtggaaa gttgtcacat ggtttcttca tgccgtatca tggtctggag
      361 taccatgcca gaagcacccc cgatgacccc tgatcgactc tctgctctga aagtggaatt
      421 ccgtcatcac ctcgaaacgt tctatgccgg acttcaactc gcccctccgt atgaaagcgt
      481 agagaaagcc atccgtacat tgacgaccat aatacatgga cttccgatcg aagaacaaac
      541 ccgaatcatg gccgatcccc gtttgcggtg gcagcagttc cgactggcct ttgacgcatc
      601 aggccttgcc aaaaaacatc gcgggatcat tgccggcctc gcccgtaatc gatcggactt
      661 gaacttgccg gcggaatacg atcagttctt aaacttgttt cttgcgtgac aggatgttga
      721 aaaagtccac cagcgcgtcc tcgcgagaca ccgccgcctc accatctcgg tggcgtccac
      781 aaacgtgacg ctcattattc ttcgcggacc tcgctgggtc ttgcctgtgg gaaggcacgt
      841 cttggcggga gaacgacgct cttttgaaca tcctgctaag agagaacacc ggctcaggtt
      901 aggaagagat ggggagcgca acggcatctc gcagctcgcc atagactttc atgagtcggc
      961 tgtcttccag cctatacgcg agggccactg tgacgagtcc gaaggccagc acaagggcgg
     1021 cgagggcaat caccgccacg cccgtaaggg ttcctcccag tgaagcgaat cctccgtcca
     1081 tggcatactg caccacgaag gtcagggacc cgatcaagac gagcgcaaac cacaggagcg
     1141 tcatgagtgc cgatgaccac ggcatacgct tcacgcacag ggtattcagc gattgatcat
     1201 gcggcgaaag atgaatcagc cctttcaccg gccaggcggt tcggaaccgc ctggaaaaca
     1261 atcgatactg agggcggatg gcaatctggg acagctgtgg ataaaaccag gccactccgt
     1321 gtggaagcat caatacaccc tcgcgagtga accgatcgcc tagggtggtg agcatagttc
     1381 caggccactg atcctggttt ttggcgacac ggcaaccgta tcggatggcg tcgggggtca
     1441 accgaataaa atacagccaa tcaccgacga tcaggccgat acataagatc cctgcgaaga
     1501 agccggccag tatcataaac gtagctatca cataggaacg aggcatgaat caaggaggca
     1561 agacacgaaa tcatcgttct gcctctatag cattagacag gtatttccgt ttcggaccca
     1621 tcaagaatct gttgactcat gaagatgaga ttggctagag tacgccggac tgcgttctgg
     1681 gcgggccttg caccatgtga atttcccgcc tcaatttctc cccgcgttgc catagcggtc
     1741 tgctgcctct gagaggatga aggcagaggc tgaatcgtgt gaggtttgga ggaaagcatg
     1801 gatcccatca agattgagcg tgtgtacacc tcttacgcgg ggttttacga caaggttttc
     1861 gggaaagtct ttcatgaagg acgtgaatcg gctatccgga acctcaatgt ccaacc
//
LOCUS       DASTWE010000655         1297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_696552, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000655 DASTWE010000000
VERSION     DASTWE010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696552"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1125
                     /locus_tag="VFT30_06810"
     CDS             262..1125
                     /locus_tag="VFT30_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEU5092377.1"
                     /translation="MLSTAAAVHLLRHSAWTKIVIRNVTIVLVLALPAWLHAGETGHS
                     TWGYDGDRGPLHWGKLGPEFSLCEKGMAQSPVDLLRAHKTTLNDIYFSYKEAPFHVVN
                     NGHTLQEVEPVSETAKSRYPKHGQTVTHFDKDSTIVFDEDLYLLEQFHFHAPSEHTID
                     HRHYPMELHLVHHNERHEVAVVAVFMEEGKHNPFFETFLEHAPAKIGEVMDDHNHSVN
                     PITLLPERRSYYLYSGSFTTPPCSEGVSWLVMQDPIEVSAVQIEKFRALVGHDNIRPT
                     QPLHKRFVLEK"
BASE COUNT          325 a          322 c          346 g          304 t
ORIGIN      
        1 cggcagtcgg attgattttt aaaatcgcac tgtgtacagt gtgcgccatc gctcgtttag
       61 atgcgtgaat gcgtggagga gaagcaccag caggcaaaag aagttgagat gagcaaagca
      121 gccgaaggtt aacactcatt caaaggcgct tcggtcccat caccggtact gtttcatgat
      181 ttgtggttga tagatccagt ctgcgataga tgatcaattg ataaaggtta taggggaggg
      241 atggtcatgg agacatgctc tttgctgagc acggcagccg cggtgcatct gttacgccac
      301 agtgcttgga cgaagattgt gattcggaac gtaacgatag tgcttgtgtt ggcgctgccg
      361 gcgtggctgc atgcaggaga gacgggacat agcacgtggg gctatgacgg ggatcgagga
      421 ccgcttcatt ggggcaagct cgggccggaa ttttccttgt gtgagaaggg catggcccaa
      481 tcaccggtcg atctattgcg tgcccacaag acgacgctta acgatatcta tttttcttac
      541 aaagaagctc cgttccatgt ggtcaacaac gggcatacgc tgcaggaagt cgaaccggtt
      601 tcggaaacgg caaagtcgcg ttaccccaaa cacggccaga cagtgaccca ttttgataag
      661 gacagcacga ttgtgtttga cgaggatctt tacttgcttg aacaattcca tttccatgcc
      721 ccgagcgaac acacgataga tcataggcat tatccgatgg aactgcatct ggtgcatcac
      781 aacgagcggc atgaagtagc agtcgttgcc gtcttcatgg aggaaggcaa acacaatccg
      841 tttttcgaga catttttgga gcatgctcca gccaagatcg gggaagtcat ggatgaccat
      901 aaccattccg tcaatccgat aactctctta cctgaacggc gctcctacta cctctactcc
      961 ggatccttca ccactccgcc ctgttcagaa ggtgtcagct ggctggtcat gcaagatccc
     1021 attgaagtct ccgctgtaca gatcgagaaa ttccgcgcgc tcgtgggtca cgacaatata
     1081 cggccgacgc aacctttaca taaacgcttc gtcctggaga agtgacccct taatatctgt
     1141 tgcttgaggc cttagaacaa cggcaccgcg ttacgggtac tgaatcatcg atctgattat
     1201 cacacgccta cgcattcctc cagggttgtg aatccgtgga gggatgcgca atgatcactc
     1261 ccaaagcctt ggcaaaggag gacacccgtc actgaag
//
LOCUS       DASTWE010000656         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_82429, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000656 DASTWE010000000
VERSION     DASTWE010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82429"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..>1022
                     /locus_tag="VFT30_06815"
     CDS             124..>1022
                     /locus_tag="VFT30_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5092378.1"
                     /translation="MHLLFRRTGLLLLALLFASTVHAEDWSRFRGPNGSGISRDTGFP
                     TEFGPGKNVIWKSSVRLGKSSPVLTEKHLFLTGHADDKLYTQCFDRQTGKLLWERSID
                     RQRKPLIHKLNEPAAITPVTDGVNVYVFFHDFGLLSYDGSGKLRWQRPLGPFTNAIGL
                     SSSPILVDGRLILQVDQYVDAYIAAFSPDTGETLWKTSRAETKGWSTPIIYRPEGKSG
                     VQILTASDRLFGAYQLKTGKRTLTADTLASAIVASPVVAGDMFYAFGYNNEDRPRPFE
                     EQVSKFDKDGDGKVSALETSDDA"
BASE COUNT          234 a          318 c          250 g          220 t
ORIGIN      
        1 ccgcgccatc atcacccatc cggacataaa actcttctta aaatgactct cactcgttgg
       61 ctgtaatttg acctgtttgt caatttccag tagcgtaacg ccatccagta aagaggtgac
      121 accatgcatc tgctcttcag acggacaggt cttcttctgc ttgctctcct tttcgcgtcg
      181 accgtccacg ccgaagactg gtctcgcttc cgagggccca acggctctgg catttccaga
      241 gacacagggt tccctaccga atttggcccc ggcaagaacg tgatttggaa atcaagcgtc
      301 cgcctgggca aatcttctcc agtcctcacc gagaagcacc tatttctgac cggccacgcc
      361 gacgacaagc tctacaccca atgcttcgac cggcaaacgg gcaaacttct gtgggagagg
      421 tcaatcgacc gtcagcgaaa gcccctgatc cacaaactga atgagcccgc cgcgatcacc
      481 ccggtcaccg atggtgtcaa cgtctatgtt tttttccatg acttcggcct gctctcctac
      541 gacgggtccg gcaagctgcg ttggcagagg ccactcggcc ccttcaccaa cgccattggc
      601 ttgtccagct cgccgatcct tgtcgatggc cgtctgatcc tccaagtcga tcagtatgtc
      661 gatgcctaca tcgcagcgtt cagcccagac accggcgaaa cactttggaa gacgagccgt
      721 gccgaaacta aaggatggtc aacacccatc atttatcgcc ccgaaggaaa aagcggtgtg
      781 cagatcctca cagccagtga tcgcctcttc ggcgcgtacc agttgaagac tgggaaacgc
      841 acccttacgg ccgatactct ggcgagcgcc attgtggcca gccccgttgt tgctggcgac
      901 atgttctacg cttttggata caacaacgaa gaccgaccaa ggccctttga agaacaggtc
      961 agcaaattcg acaaagatgg cgacggcaag gttagtgctc tggagacgag cgatgacgcc
     1021 tg
//
LOCUS       DASTWE010000657         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_664163, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000657 DASTWE010000000
VERSION     DASTWE010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664163"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFT30_06820"
     CDS             <1..325
                     /locus_tag="VFT30_06820"
                     /inference="COORDINATES: protein motif:HMM:NF037477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HEU5092379.1"
                     /translation="TTCLTLYEKPLKDFTVENLRVMIGQSIGLEFLIPLAIEVLEQNP
                     FAEGDYYPGDLLSMVMQVEPNFWQTHQDLYWSVSEIVAGLPSIMKNLTDAIHRFDELG
                     SFKDG"
     gene            complement(322..732)
                     /locus_tag="VFT30_06825"
     CDS             complement(322..732)
                     /locus_tag="VFT30_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092380.1"
                     /translation="MIRRKSPKKGRFEDLFWELVSVPQLLLNPNFSLEVLMISQEEVR
                     RYEGKRRWRTRGWVIEGRQLLDVLDRRLFGATSDWLRFLPPGLESFTTVDLATRMNTR
                     RDLAQKMAYCLRESRMIDLIGKRGRSNLYRIAAP"
     gene            719..1318
                     /locus_tag="VFT30_06830"
     CDS             719..1318
                     /locus_tag="VFT30_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092381.1"
                     /translation="MRRITAPPLLAADPDGLYEYPAELLGKKNTVHIDGSIIKKDKDA
                     LNKIKDNLQKAFDKINAGADKLTTEQTQSIGRMKGISVSANGPIGMVGGTFLITQRMA
                     ENPNVDKLSGDIIHDSRHAEQAKRGLSYNEETVIPMEMEASQFTVGVMNNIRGWDARV
                     LKGYELDAATGHLPNRKWKDKSTPKSRTRVFEKMRGPRN"
BASE COUNT          363 a          332 c          351 g          295 t
ORIGIN      
        1 gacaacctgc cttacgcttt acgagaaacc gctcaaggat ttcacggttg agaatctgcg
       61 cgtgatgatc ggacagagta ttggcctgga attccttatt ccgttggcta tcgaagtgct
      121 ggaacagaat ccctttgctg agggtgatta ttatcctggc gatctacttt caatggtaat
      181 gcaggttgaa cccaacttct ggcaaaccca tcaggacctt tattggagcg tgtctgagat
      241 cgtagcgggg ctcccgtcga tcatgaagaa tcttacggac gcgattcacc gatttgatga
      301 actgggttcc tttaaggacg gttaaggcgc ggcgatgcgg tacaggttgg accgcccgcg
      361 cttacctatc aagtcgatca tccggctttc acgcaggcag tacgccatct tctgggcgag
      421 gtctcttctc gtgttcatcc gggtggcaag atcgaccgta gtgaatgact ccaatccggg
      481 tggaagaaac cgcagccaat ccgacgtcgc gccaaacaaa cgccgatcga ggacatcaag
      541 caattggcgt ccttcaataa cccatcctct tgtgcgccat ctgcgcttgc cctcgtatcg
      601 cctcacctct tcctggctga tcatcaagac ttcgagcgaa aagtttggat tcaatagcaa
      661 ctgcggaaca ctaacaagct cccagaaaag atcctcgaac cggcctttct tgggtgactt
      721 gcggcgaatc actgcaccgc cattattggc cgccgaccct gatggattgt acgagtatcc
      781 ggctgaactc ctggggaaaa agaatacggt ccacatcgac ggctcgatca tcaagaagga
      841 caaggatgcg ctcaacaaga ttaaggataa cctgcaaaag gctttcgaca agatcaacgc
      901 cggtgccgat aaattaacga cggaacagac acagtccatc ggaaggatga aaggtatttc
      961 tgtttctgcg aacggcccaa tcggaatggt ggggggcacc tttctaatca cgcagcgaat
     1021 ggctgagaat cccaacgtgg acaaactctc aggggacatc attcacgatt cccgtcatgc
     1081 ggagcaggct aaacgagggc tgagttataa cgaggagacc gtcataccaa tggaaatgga
     1141 agcgagtcaa ttcacggttg gggttatgaa taacatacgc ggatgggatg ccagagttct
     1201 gaaaggctat gaacttgatg ccgcaaccgg tcatctccca aatagaaaat ggaaagataa
     1261 gagcacaccc aagtccagga ccagagtctt tgagaaaatg agagggcctc gtaattgagt
     1321 ggaggataat aagcgatgaa a
//
LOCUS       DASTWE010000658         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_147145, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000658 DASTWE010000000
VERSION     DASTWE010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147145"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..>1236
                     /locus_tag="VFT30_06835"
     CDS             27..>1236
                     /locus_tag="VFT30_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092382.1"
                     /translation="MTSFDGDGSSKQPVRTLGTVVDVTHHKNVEARRRETARMEAIGT
                     FAGGIAHELNNGLTAVLGFSELALPLIPAETKAHRHIRQVITAAKKSRELIQQLLAFG
                     GQNDQGRCPLVLHSLAKESLRFLRPTIPLWIELRAQVAQATNPISANAVQMHEMIFNL
                     VDNAIRAMQSTGGILDVQLQNQEFVTDQIMPSGRLLAGRYVCLSVRDTSEGMDPERIS
                     RLFASFVTSKTGSERQDVGLAIVYGIVTAHGGTLTVESQIGAGTTVSIYLPTLPTCAP
                     STIQEDDPLPHGHECILFVDDEDSVVRLGREVLESLGYYPVVCRTAVEALEVFQAEPK
                     RFDLLITDRTMPGMSGDRLARECRRLRPDLPVMLCSGSNGVPGLDDACCQDVAECLLK
                     PLTLHELAYAI"
BASE COUNT          264 a          331 c          356 g          285 t
ORIGIN      
        1 ccatgcggca cattagtctt tgttctttga cttcgtttga tggcgatggt tcctcgaagc
       61 agccggtccg cacacttggg acggtcgtcg atgtgaccca tcataagaac gtcgaggctc
      121 ggcggcgcga gacagcgagg atggaagcga tcggcacctt tgccggtggt atcgctcacg
      181 agttgaataa cggcctcacg gccgtactcg gcttcagcga gttggcgctt ccgttgattc
      241 cggctgagac caaggcacat cgccatatta ggcaagtcat aacggcggcg aaaaaatcac
      301 gggagctgat ccagcagttg ctggccttcg gtgggcaaaa cgaccaaggt cgatgcccac
      361 tggttctcca ttctttggcg aaagaatccc tcagattcct tcgtccgacg atccccttgt
      421 ggattgaact cagagcgcag gttgcacagg cgaccaaccc tatttcagcc aatgccgtgc
      481 agatgcacga gatgattttc aacctggtcg ataacgccat tcgtgcgatg cagagcacag
      541 gaggaatcct cgatgttcag cttcagaacc aggagtttgt aacggatcag atcatgccgt
      601 ccggtcgact tctcgctgga cggtacgtct gcctgagcgt tcgggataca agcgagggca
      661 tggatcctga gagaatcagt cgattgttcg cctcatttgt gacgtccaag actggcagcg
      721 agaggcagga tgtaggacta gcgatcgtat acggcatcgt cacagcgcat ggcggtactc
      781 tgacggttga gagtcaaatt ggcgccggta cgacggtatc gatctacctt cccactctcc
      841 cgacttgtgc tccgtcgacg attcaggaag atgatccgct tccccacgga cacgaatgta
      901 tcctattcgt cgatgacgaa gactccgttg ttcgcttggg aagagaggta ttggaatccc
      961 tcggctatta tccggtggta tgtcggacgg ctgtcgaagc attggaagtc tttcaagccg
     1021 agcccaagcg gttcgacttg ctgatcaccg atcggacaat gcccggcatg agtggggatc
     1081 gtctggctcg agaatgtcgg cgacttcgac cggatctccc cgttatgctc tgcagcgggt
     1141 caaatggtgt acctggtctg gacgacgcct gttgccagga cgttgcagag tgcctcctaa
     1201 aaccgcttac gttgcatgaa ctggcgtatg ccatcc
//
LOCUS       DASTWE010000659         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_793312, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000659 DASTWE010000000
VERSION     DASTWE010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793312"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFT30_06840"
     CDS             <1..1215
                     /locus_tag="VFT30_06840"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEU5092383.1"
                     /translation="TSGMASMASLMAILGGLDMLDTSISPLAGGTSHPATETLVASLQ
                     NTPYDTGLELASFQPITEHFRTVRRKYRQFESDFTGVDAEILMSQIPGGMLSNLAAQL
                     TEQNALDRMKEVLDEVPRVRKEMGYPPLVTPTSQIVGTQATLNVLTGERYKVITTETK
                     NYFLGLYGRAPGQVDLDVMARATGDETPIKIRPADRLEPELDDAKKELPASAQSIEDQ
                     LSFVLFPTIARDFFEARENGDLTPEPLELGATKGPAPAHELHLAPVEFNVTVHGETYH
                     VKVSGSGRKVDGRKPYYIRVNDKLEEVSLEPIQEVLAGVPESQETESGGKPKRPRPSK
                     PGDVTPPMPGRVVKILVAVDDRVKAGDPLLIIEAMKMESRVPAPIDGRVVMILAVEGD
                     NVKTDETVIQLE"
     gene            1190..>1819
                     /locus_tag="VFT30_06845"
     CDS             1190..>1819
                     /locus_tag="VFT30_06845"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5092384.1"
                     /translation="MKRSFNWNSSITDSIPYPCRTWRLVALTLCLVSYLMTACAQPTQ
                     IPPYFETLERTPIERIPIKTVLVHDQRIAYLDIGNGPPVILIHGFGGSMWQWEHQQHD
                     LSQHFRTLTLDLPGSGLSDKPDIDYLPDQMLNFCIGFMDALQIPQATLVGNSMGAGLA
                     IGMTLTHPTRVGKLVLISGLPSHVMAKLASRSFRQALESRAPSWLVFFGN"
BASE COUNT          417 a          532 c          485 g          385 t
ORIGIN      
        1 acgtccggca tggcttccat ggcctcattg atggcaatcc ttggaggcct cgacatgctc
       61 gatacctcca tctctccgct ggcaggggga acctcccatc cggcaacgga aacgctggtc
      121 gcttcacttc agaatacccc ctacgatacg ggactggagc ttgcctcttt ccagccgatc
      181 acggagcact tccgaacggt tcgccgaaaa tatcgccagt tcgagagcga ttttaccggc
      241 gtggatgcag aaatcctcat gtcgcaaatt cctggcggta tgttgtcaaa cttggccgca
      301 cagctgacag agcagaatgc tctggaccgg atgaaggaag tcctcgacga agtcccgcgt
      361 gtgcggaaag aaatgggcta tcccccgctg gtcaccccga ccagccaaat cgttggaacg
      421 caggcgacgc tcaatgtact gactggtgag cggtacaaag tcatcaccac tgaaaccaag
      481 aactatttct tgggattgta tggccgtgcc cctggacagg tcgatctcga cgtgatggcg
      541 cgcgcgaccg gcgatgaaac gccgatcaaa atcagaccgg ctgatcgact cgagccggaa
      601 ttggatgacg cgaaaaaaga gcttcccgct tcggctcaaa gtatcgaaga tcagctgtcc
      661 ttcgtactgt ttcccaccat cgcgcgagac ttttttgaag cccgtgagaa cggcgatttg
      721 acccctgagc cgctcgaatt gggggcgacg aaagggccgg cacccgctca cgaactgcac
      781 ctggctcccg tggaattcaa tgtgacggtc catggtgaaa cctatcatgt gaaggtctcg
      841 ggctccgggc gaaaggtcga cggacgtaag ccctactaca tccgtgtcaa cgataagctc
      901 gaagaagtgt cgctcgaacc catccaggag gtgcttgccg gggtcccgga gtcccaggag
      961 acagaatcgg gaggaaaacc caaacgtccc cgaccttcga aaccaggcga tgtgactccg
     1021 cccatgcccg gtcgagtggt gaaaatcctg gttgcggtcg atgatcgcgt caaggccggc
     1081 gatcctctgt taatcatcga ggcgatgaag atggaaagcc gggtgccggc gccgatcgat
     1141 gggagagtcg tgatgatcct ggctgtcgag ggtgataatg taaagacgga tgaaacggtc
     1201 attcaactgg aatagttcca tcaccgactc aatcccatac ccctgcagaa cttggcggct
     1261 tgtggccctc acgttgtgcc tcgtatccta tctcatgacc gcttgtgccc agcctacaca
     1321 aatcccgccc tattttgaaa cgttagaacg taccccgata gaacgtatcc cgatcaaaac
     1381 cgttctcgtc catgatcaac ggatcgcgta tcttgatatc ggcaatggcc caccggtcat
     1441 cttaatccat ggctttggcg gatccatgtg gcaatgggaa catcagcaac atgacttgtc
     1501 tcaacatttc cgtaccctga cgctcgacct accaggttcc ggactgtccg acaaaccgga
     1561 cattgattac ctgccggacc agatgctgaa cttctgtatc ggattcatgg acgccctgca
     1621 aattcctcaa gccacactgg tcggcaattc catgggcgct ggattggcca taggaatgac
     1681 attgacccac ccgacccgtg tcggcaaact cgtactcatc agcgggttac cgtctcatgt
     1741 catggccaag ctcgccagcc ggtcgttcag gcaagctctg gagtctcgag ctccttcctg
     1801 gctggtcttc ttcggcaac
//
LOCUS       DASTWE010000660         1355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_955006, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000660 DASTWE010000000
VERSION     DASTWE010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955006"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VFT30_06850"
     CDS             <1..954
                     /locus_tag="VFT30_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HEU5092385.1"
                     /translation="TSTVTVAFLKEAPFVTKEESMAIRLFKDKNCKDESRVAVSDSRD
                     LKGLIADKPSSLRIDDEDETILLFKNDDWHGGALYIRGPATVTDLGSEKEGGRMGFGN
                     SIRSVRVTPFQVDLNVTVVKDGKDNLPGDWKTQPQAKEDIHKMVDAANIFLADKRALL
                     QLAIARITFRNSNNLFAISKLEQIVLPSEWTEKGELDVVFTHRFTKEGTIGRAFFPCW
                     GESVVVAKRVNSVVGADISLTLDEMAAVMMHEIGHHFGLGHGTANTEKNLMFASLEQD
                     SLSEMWLLSDQIREMHDRLANNISRQGERKDPRPGVANPTP"
BASE COUNT          340 a          359 c          375 g          281 t
ORIGIN      
        1 acatcaacag taacggtcgc cttccttaaa gaggcaccgt tcgtcacaaa ggaggaatcc
       61 atggccatca gattattcaa agacaagaat tgcaaggacg aaagccgcgt cgcagtcagt
      121 gactcgcgcg acttaaaggg cctgatcgct gacaagccct cctcgctgcg catcgacgat
      181 gaggacgaga caattttact gtttaagaac gacgattggc atggcggggc gctgtatatc
      241 cgtggtcccg ctaccgtgac cgacctggga tcagagaagg aaggcggacg catgggattt
      301 ggcaactcca tccgctctgt ccgtgtcaca ccctttcagg tcgatcttaa cgtcactgtg
      361 gtcaaggacg gaaaggacaa tctccccggc gattggaaga ctcagccaca ggcgaaggag
      421 gatatccaca agatggttga tgccgccaat atctttctgg ccgacaaacg ggcgctgctg
      481 caactggcga ttgcacgtat caccttccgt aattcgaata atttgttcgc aatctcgaaa
      541 cttgaacaga tagtcctccc cagcgagtgg acagaaaaag gtgaacttga tgtggtcttt
      601 acccaccgct ttaccaagga gggcacgata ggacgcgcct tcttcccctg ctggggcgaa
      661 agtgtggtgg tagccaagcg ggtgaacagt gtagtcggag ctgacatcag cctgacgctc
      721 gacgagatgg ccgcggtaat gatgcacgag atcggccacc atttcggcct tggtcacggc
      781 accgctaata cggagaagaa cctaatgttc gcgagtctgg agcaagatag cctgtccgaa
      841 atgtggctac tgtcggatca gatccgcgaa atgcacgacc gtctggcaaa caatatcagc
      901 cgccaaggcg agcgcaagga tcctcgtcca ggagttgcca accccacgcc ttgagatgga
      961 cacgatatca ggtttggccc aatgcctagc ctggtgcgca tccacgatac agggagtcac
     1021 gcgcctgccg cggggcaatt cagagctcgg agtgacttga gccttcccga cgaagcagct
     1081 ttggcgggaa ggctaggaag ccgtgacctc ctatgaaagc tggagagacg caattgagca
     1141 tctgagagat cgctgccgtc ccgtccctat gaagctatcg tgccggacat ggtccggctg
     1201 ctgaggcaag agagcaccca agaaatgcga tgcttagcgg tcgcgccatg gaactaaatc
     1261 gctcatcgac atgcacagaa taagatgtga ttcaaattct tgtaaggatc tgacactttt
     1321 ctttacgaca gctgacctct gctttttctt tatga
//
LOCUS       DASTWE010000661         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1019853, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000661 DASTWE010000000
VERSION     DASTWE010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019853"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1123
                     /gene="miaB"
                     /locus_tag="VFT30_06855"
     CDS             <1..>1123
                     /gene="miaB"
                     /locus_tag="VFT30_06855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU5092386.1"
                     /translation="NTCAIRENAHNKIYAHLSELKTIKKQRPLVVGVLGCMAQNLKSD
                     LATKEPLIDVLAGPDAYRQLPSLLTTALQAQELGLSQKGFALDLSEYETYEGIMPDRN
                     SGVNAWITVMRGCDNFCSFCVVPYTRGRERSRDPESVLLEARDASVRGFKQVTLLGQN
                     VNSYSHEGWDFARLITAVADTAGIDRVRFTSPHPKDFPAALIEAIATHPKICKHIHLP
                     LQAGSDRILELMGRTYTGAEYLALVDRIRHTIPDVILTTDIICGFCSESDEDFRATFR
                     MVEQAQFDSAYIFKYSERKHTIAARKYADDIPDQVKGMRVAQLVEMQRRITLERNRRY
                     IGKDMDILVEGDATRSSTQAMGKTDGNITVVWNKSTGDF"
BASE COUNT          277 a          319 c          288 g          239 t
ORIGIN      
        1 tcaacacctg cgcgatcagg gagaacgcac acaacaagat ctacgcccat ctctccgagt
       61 tgaaaacaat caagaagcag cgtccgctcg ttgtcggcgt ccttggctgc atggcgcaga
      121 acttaaagag tgacctagcc accaaggaac cgctcatcga tgtcttagct ggcccggatg
      181 cgtaccgtca acttccctcg ttgctgacga ctgcgcttca agcacaagaa ctgggattat
      241 ctcagaaagg gtttgccctc gatctgtcgg agtatgaaac ctatgaaggg atcatgccgg
      301 accgtaacag cggggtgaac gcctggatca ccgtaatgcg aggctgcgat aacttctgct
      361 ccttctgtgt cgtcccctat accagagggc gtgaacgatc gcgtgatccg gagtccgttc
      421 tgcttgaagc tcgtgatgct tctgtccgag ggttcaagca agtcacctta ctcggccaga
      481 acgtcaactc ctacagtcat gaaggatggg acttcgccag gctgatcacc gccgtggcgg
      541 atacagcagg gatcgatcga gtacggttta cctctccgca ccctaaagac tttcctgccg
      601 cactcatcga ggccattgcc acgcacccca aaatctgcaa gcacattcat cttccccttc
      661 aggcagggag cgatcgtatc ctcgaactga tgggacgaac ctatacggga gccgagtatc
      721 tggcgcttgt tgatcgcatt agacacacta tccctgatgt gattctcacc acggatatta
      781 tctgcggttt ctgttccgag tcggatgagg acttccgggc cacgtttcgc atggtagaac
      841 aggcacagtt cgactcagcc tatatcttca aatactcgga gcggaagcac acgatcgcgg
      901 caagaaagta tgccgacgat atccctgatc aagtcaaagg catgcgcgtc gctcaactcg
      961 tagaaatgca acgccgcatc accttggaac gcaatcgccg atacatcggc aaggacatgg
     1021 acatcttggt cgaaggtgat gcgacccgct cctcaaccca ggcaatgggg aaaaccgacg
     1081 gaaatatcac cgtcgtatgg aataagagca ccggtgactt tag
//
LOCUS       DASTWE010000662         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1569009, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000662 DASTWE010000000
VERSION     DASTWE010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569009"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFT30_06860"
     CDS             complement(<1..741)
                     /locus_tag="VFT30_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEU5092387.1"
                     /translation="MNIFRFLLDVLRSFLQQGCASLAASLAFFSLLSLFPLAFLLLYG
                     VSFFVSQDVIGEQFMLSFLKEFLPSLGERLVEELHRISVLESVRWLVFLSFFWFGGLV
                     FYELDYTLNVVFESMPERHPLISTAISIALVGCTGLLLLTAYVGTQTITFLTDYAPRL
                     WGLDLVALAAHDFHLTYTLPFILAFLAVSLLYRLVPRRRPRWRAAMAGALTFSVLWVS
                     AKLLFVSYSDYATVYARLYGSLLEVVLLL"
     gene            complement(778..>1450)
                     /locus_tag="VFT30_06865"
     CDS             complement(778..>1450)
                     /locus_tag="VFT30_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5092388.1"
                     /translation="GLKANWSAIFLLLCLATLVLGNVVALVQTNIKRMLAYSSIAHAG
                     YALIGVVAAGRLENSSGIASVLLYIALYTFMTFGAFAIVAMLRKDGMEGDEIEDFTGL
                     SKRHPVAALLMLIFMVSLAGIPPTAGFIGKFYVFMSAVEAGMTWLAVIALVFAAVSAY
                     YYLRLVMVMYMRERSDVADSAPQLVMSPTLSIVLACAVAGVVIFGIYPNPLVQLAMQA
                     VLTLR"
BASE COUNT          423 a          391 c          402 g          234 t
ORIGIN      
        1 caacaagagg acgacttcca gcaatgatcc atagagtcga gcgtagactg tagcatagtc
       61 gctatagctg acgaacagca gcttggccga gacccagagt acgctgaaag tcagtgctcc
      121 ggccattgcg gctcgccacc gtggacggcg gcgaggaaca agacggtaca acaggctgac
      181 cgccaggaag gcgaggataa agggtagagt ataggtcaga tgaaaatcgt gcgcggccag
      241 tgcaacaagg tcgagtcccc agagcctggg tgcgtagtcg gttaaaaagg tgatggtctg
      301 cgtcccaaca tacgccgtaa gcagcagaag accggtacat ccgaccagcg cgatggagat
      361 tgccgttgaa atcagcggat ggcgctcagg catgctctca aagaccacat tgagggtata
      421 gtccaactca taaaagacga ggcctccaaa ccaaaaaaac gataaaaata cgagccatcg
      481 cacactctcc aacacactga tccgatggag ctcttctact agacgctcgc ccaacgacgg
      541 caaaaattct ttgaggaagc tcagcatgaa ctgttcaccg atgacgtcct gactgacgaa
      601 gaaactcacc ccgtacagga ggagaaacgc gagcggaaag agcgaaagca gcgagaaaaa
      661 ggccaaggat gcagcgagac tggcgcagcc ctgttgcaaa aaggatctca ggacgtcgag
      721 aaggaagcgg aagatattca tgaggctccg catgcggatg gacaacacac ggctgtctca
      781 tcttaacgtc agcaccgcct gcatggcgag ctgtacgagc ggattaggat agataccgaa
      841 gatcaccact ccagccaccg cgcaggccag tacgatggag agcgtcgggg acatgaccaa
      901 ctgaggagcg ctatcggcga cgtcactccg ctcgcgcatg tacatgacca tcaccagtcg
      961 gagatagtaa taggccgaca cggcggcaaa aaccagcgca atgaccgcca gccacgtcat
     1021 gccggcttcc acggccgaca taaacacgta aaattttccg ataaaccccg cagtaggagg
     1081 aatgccggcc aaagagacca tgaaaatcaa cataagcaat gctgccactg gatgtcgttt
     1141 ggacagaccg gtgaagtcct cgatctcatc tccttccata ccgtctttgc gaagcatggc
     1201 gacgatggca aatgcgccga aggtcatgaa tgtatagagt gcgatgtaga gcaacacgct
     1261 tgcgataccc gaagagtttt ctaaccgtcc ggcagccacc acgccgatca aggcatagcc
     1321 ggcatgggcg atactcgaat aggcaagcat ccgcttgata ttcgtctgca ccaacgcaac
     1381 gacatttccc aacaccaggg tggcgaggca gagcaggagg aagatggcgg accaattggc
     1441 cttcaagccg
//
LOCUS       DASTWE010000663         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1116934, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000663 DASTWE010000000
VERSION     DASTWE010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116934"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFT30_06870"
     CDS             complement(<1..653)
                     /locus_tag="VFT30_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5092389.1"
                     /translation="MEADVRRILQELVMAEPLALNRLDRERLVLDVEHEVFGLGPLEV
                     LMSDPEISDILVNDHQTVFVERHGKIEETDVRFRDEAHLMQIIGRIVSRVGRRIDEHS
                     PMVDARLPDGSRVNAIIPPLAIDGPILSIRRFGVERLTMKSLIELNTMPQHFAEVLEA
                     CVKSRLNILISGGTGSGKTTLLNCLSNYIPPTERIVTIEDSAELKLQQRHVLRLETRP
                     "
BASE COUNT          299 a          354 c          370 g          317 t
ORIGIN      
        1 ggcctggtct ccagccgcag gacatgtctt tgctgcaact tcaattcggc agaatcttcg
       61 atggtgacga tccgttcggt gggcggaatg tagttggaaa ggcaattgag caatgttgtt
      121 ttgccggagc cggtaccgcc ggaaatcaaa atgtttagcc gtgacttcac acaagcttcc
      181 aatacttcgg cgaagtgctg cggcatggta ttgagttcaa taaggctttt catcgtcagt
      241 cgctcaacgc cgaaacgacg gatggataga atcggtccgt cgatggcgag aggcgggatg
      301 atcgcattga cgcgcgaccc gtccggcagg cgggcatcga ccatcggcga atgttcatca
      361 atgcgacggc cgactcggct gacgatgcgg ccgatgatct gcatcaggtg cgcttcgtca
      421 cgaaagcgaa catcggtttc ttcgattttg ccgtgtcgct cgacaaacac ggtttggtga
      481 tcgttgacca aaatatcgct gatctcgggg tcgctcatca gcacctcgag agggcccaag
      541 ccgaagactt cgtgctctac gtcgaggaca aggcgctctc ggtcgagccg gtttaacgcc
      601 agcggttccg ccatcaccaa ctcctgcaga atccgccgca catcggcttc cattctcgga
      661 gtttccaggg ttgccagctt cgccatatct aaagcttcga gaagctggcg gtgaatgcgc
      721 atcttgagtt cgtcgtagac attacgaccg acggcatgtg gctccaacgt ggcagtgaga
      781 ttcttacctt cccgttgctc gagtagtttg cgtaacgagt ctttcatggt tgcctccacc
      841 aagttctctc ccgacgtcat gtgcaactaa cgtaccaatc atggtccgga aacgaatgcc
      901 gacttgagcc gaagccacgg tttctctgac cagataaact gcggcgctac cgagtggcat
      961 tgccagtcac gataaagtct tactgatgcg cggccgaaaa gtttcgctct gaacttaccg
     1021 ggactaccaa aacactgatg atccggtcgg atccaaggtt ttgcagacct tacaacgttg
     1081 actgctgggg atggttcaac aaagcttgta ggcgggtgga acgatggatt ttttcgcgcg
     1141 aattctcgac tgtgataatc gcttctcgcg agattgcgaa taattgaaca acgacttgac
     1201 gatgtcgcga tgccgacgat gtccggtctc gagctacgcc tgactggctg gcagataggg
     1261 gcgccaacca tggttccaat cgagaaattc gagcccgctc aaacgtttct tgtaatcgta
     1321 agcaaacggc tcttggtagg
//
LOCUS       DASTWE010000664         1200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1052288, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000664 DASTWE010000000
VERSION     DASTWE010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052288"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..809)
                     /locus_tag="VFT30_06875"
     CDS             complement(321..809)
                     /locus_tag="VFT30_06875"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU5092390.1"
                     /translation="MIVQRLLKYCFDLAGITLVVLMLVTVVDIAARHLGLFTVRGIIE
                     ISTLAVVMIGFLALPYSFVLGGHIVVDLATLYLPPNVNRTIDKAWTVFAGFCLAFLAI
                     LMWQATFRAYHTNDISLDLQLPLVWFWAAASVGMTLAPVACLIALRRSSKETAVNEMT
                     SA"
     gene            complement(806..>1200)
                     /locus_tag="VFT30_06880"
     CDS             complement(806..>1200)
                     /locus_tag="VFT30_06880"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU5092391.1"
                     /translation="NREVSEYLAHLQLPPAAVMTIILIGLILLGCVMDSTSILFITTP
                     FLFPVIVSLGYDGVWFGIVMTMVIQIGLIHPPFGLNVFILSAMIKEVTVAEAFWGCVP
                     FLIASLLMIVLICVYPEFILWLPNRLFG"
BASE COUNT          306 a          309 c          361 g          224 t
ORIGIN      
        1 aagttgattc taaggtgttg gtggtcgctt ttatttgcaa ttgggatccc agctgctgcg
       61 acgaatcgcg ccagcaagca gagcgaccga cggacgctcc ggcgcccgcc gcggccacgc
      121 aaccgatagg gccggcgccg tatcggaatt cgcgtgcgcc caggcgcgca tagaatgtcg
      181 gcggggacgt gcggaatggg cgggcttttc tgctcggcgg actggatgcc tttgcgcagg
      241 tagttgttgc agtcgaattt tcatgtgtca cggcggcgcg cgagcacggc gttgtcgaac
      301 ggcgattgag tcccggctcg ttatgccgaa gtcatttcat tgaccgctgt ctccttggat
      361 gaacggcgta acgcaatcag gcaggcgacc ggtgcgaggg tcatacccac ggatgcagca
      421 gcccagaacc aaacaagagg caactgtaaa tcgagagata tgtcattggt gtgatacgcc
      481 cggaaggttg cctgccacat cagtatggcc agaaaggcaa ggcaaaaccc ggcgaagacg
      541 gtccaggctt tgtcgatcgt ccgattcaca tttggtggaa ggtacaaagt ggccaagtct
      601 acgacgatgt gaccgccgag gacgaaggag taaggcagcg ccaggaagcc gatcatcaca
      661 acggccaggg tagagatttc gatgatgccg cgaacagtga agagtccgag atggcgcgct
      721 gcaatatcca ccaccgtcac cagcatcagg acgacgagtg tgatgcccgc caaatcaaaa
      781 cagtatttga gcaggcgctg cacgatcatc cgaagagcct gttgggtagc caaaggatga
      841 attccggata tacgcaaatg aggacgatca tgagcaaact cgcaatcaaa aacggcacgc
      901 agccccagaa cgcctctgcc accgtgactt ccttgatcat ggctgacagg atgaacacgt
      961 tcaggccgaa agggggatgg ataagtccga tttgaatcac catggtcatc acgattccga
     1021 accagacgcc gtcataaccc aatgagacga taacgggaaa caggaaaggt gtggtgataa
     1081 agaggatcga tgtcgagtcc atcacacagc cgagaagtat caggcctatg aggatgatgg
     1141 tcatcaccgc cgccggtggt aattgaaggt gcgcaagata ctcgctcacc tctcgattta
//
LOCUS       DASTWE010000665         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_922913, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000665 DASTWE010000000
VERSION     DASTWE010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922913"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..645
                     /locus_tag="VFT30_06885"
     CDS             175..645
                     /locus_tag="VFT30_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092392.1"
                     /translation="MTKVLRMGLQIVPLLLIGNLIGCAWMNGMQERSALCSYDHAWDA
                     AVAAVKDRSADTMDKEAGLIVTQWLEIPMPGRTYGVFRREVSDSRDRSRLTLKVKQMD
                     DVARISFVEDRQSWAFRGGSRLFGWARTEPSEEVMRDVQNRIDAKLKERGCTVG"
     gene            630..1316
                     /locus_tag="VFT30_06890"
     CDS             630..1316
                     /locus_tag="VFT30_06890"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEU5092393.1"
                     /translation="MHRRLSIIAGVAIGATLWLVAPIPAGAEKVWDNDLHRYLTEAEL
                     NHAEVFMSEEDAVKIMLPKSERVRKDVIQLTQDRKDFVEQRIGWKFPEQAFEVYIGET
                     GDKIDGYAMVHNTIGKHKHMTYMVGVDATGACSDVELLVFREARGSEVGRKRFNAQYE
                     GKTVLDPIRINKDIINITGATMSVRSISAGVKRVLVLVDEFYLKPAGIGSDTLAAKKD
                     KGFFSSIFGN"
     gene            1342..>1857
                     /locus_tag="VFT30_06895"
     CDS             1342..>1857
                     /locus_tag="VFT30_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092394.1"
                     /translation="MRNFNQKFRLRDSDRQIRMLYTLFLLLMFVGFCFSFFWAHSMTG
                     LSPQGITEHYRGSDATFGEPMSFRELAEITHFHLFTMPVVFMILIHVMYLTSASHTLK
                     TIVTWAGFGGVILDLLSPWLISYVSPIFVVSMLAGDTLMTISFLVMMVVPLYEMWVLE
                     QPLIGGKRPQES"
BASE COUNT          437 a          471 c          539 g          410 t
ORIGIN      
        1 caaaatcaac taccagcata atacgcagaa cggagcagtg ggcttagtac ccgccggaga
       61 tttgggcacg gccaatagtc cgatggacgg agatggcgtc ctcttccagg cggcgacata
      121 cttttaactc tattgaaagg aggctccccg ccggaccggc ggggagttcg tgacatgacg
      181 aaggttctgc gaatgggctt gcaaattgtt ccactccttt taatcggaaa cctgatcggt
      241 tgtgcctgga tgaacgggat gcaagaacgg tctgccctgt gcagctacga tcatgcctgg
      301 gatgcggcag tcgctgccgt gaaggatcgc tcggcagaca caatggataa agaggccgga
      361 ttaatcgtga ctcaatggct cgaaattcca atgccgggta ggacctacgg ggtgttccgg
      421 agagaagtct cggacagcag ggatcggtcc cgtctcactt tgaaagtaaa gcagatggac
      481 gacgtcgcca gaatctcgtt tgtcgaggac cgacagagct gggcatttcg aggcggatcg
      541 cgacttttcg gctgggcacg cacagagcct tccgaggagg tcatgcgcga cgtgcagaat
      601 cggattgacg ccaaactaaa ggagcgcgga tgcaccgtcg gctaagcatt atcgcgggcg
      661 tcgcgatcgg agccacactc tggctggtcg cgccgattcc ggccggagca gaaaaggtat
      721 gggacaatga cctgcaccga tacctgacgg aagctgaatt gaatcatgcg gaagtcttca
      781 tgtcggaaga agacgccgtg aagatcatgc tgccgaaatc agagcgcgtc cgcaaagacg
      841 tgatccaact cacgcaagat aggaaagact tcgttgagca acgcatcggc tggaagtttc
      901 cagagcaagc tttcgaagtc tacatcggtg aaacgggcga taagatcgac ggctatgcca
      961 tggtgcataa cacgatcggt aaacataagc acatgaccta catggtcggc gtggatgcca
     1021 caggcgcctg ttcagacgtc gaactactgg tatttcgcga ggccagagga agcgaagtcg
     1081 gacggaaacg attcaacgcg cagtacgaag gcaagacggt cctggacccg attcgtatca
     1141 ataaggacat catcaatatc accggagcca cgatgtccgt ccgctccatc agcgccggcg
     1201 tcaagcgggt gctcgtcttg gtcgatgagt tttacttgaa gcctgccggg atcggcagcg
     1261 atacgctggc ggcgaaaaaa gataagggct tcttctcatc catattcggg aattagctca
     1321 agcggaccca gcgtcccagg catgcgaaac ttcaaccaga aatttaggct gcgcgattcc
     1381 gatcgccaga tccggatgct gtacacgttg ttcttgcttt tgatgttcgt cggattttgc
     1441 ttttcttttt tttgggcgca tagcatgacg gggttgtccc cgcaaggcat cacggaacac
     1501 tatcgaggat ccgatgcgac attcggcgaa ccgatgtcgt ttcgagagct ggcggagatc
     1561 acgcactttc atctctttac gatgccggtc gtgttcatga tcttgattca tgtgatgtac
     1621 ctaaccagtg ccagccacac cttgaaaacg atcgtgacct gggccggctt cggcggcgtg
     1681 attctcgacc tactctcgcc ctggcttatc agctatgttt ctcctatttt cgtcgtttcg
     1741 atgttggcag gtgacacgct gatgaccatc agtttcctgg ttatgatggt ggttccactg
     1801 tatgaaatgt gggtcctcga gcagcccttg attggaggga aacgaccaca ggagtcg
//
LOCUS       DASTWE010000666         1462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1375333, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000666 DASTWE010000000
VERSION     DASTWE010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375333"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VFT30_06900"
     CDS             <1..1111
                     /locus_tag="VFT30_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092395.1"
                     /translation="VVKNEDANKQSNAILDDLQKDPNANRLKQGIELLDQLKKEGKIS
                     EADADAAKNSLLKTFQDSSNAKTPMGNEEIGKLADKSKQTNKDISVTRPGGETVEIKN
                     PATEGSFSSASNAVGTTLDMVAGGGTLKFLPTGQTVAVDPTPAQMKIIADKDGWVPTK
                     MSDYLTLAVGGRLIGLRTFTGVLRYLSLVPQGSVQTLNMVCHCPDNLTLAFQRDFVFS
                     EQSGVTTIGPPDPQDGPFEEVTIPELMQLNELDSIDGDSGLIIGLADVRAAFAANAEL
                     RLFCIGRGANEQIVQTLANLLQVKIIAFTDAVIAFRVEVLEGATVGNRPALSKRILAT
                     PRFSSGAPFVYNNFEQMVSEDAAKTGKLIILPRRS"
     gene            1145..>1462
                     /gene="tssD"
                     /locus_tag="VFT30_06905"
     CDS             1145..>1462
                     /gene="tssD"
                     /locus_tag="VFT30_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HEU5092396.1"
                     /translation="MLSAYLKLEGDTTGKIRGPVRDRDSDKDGSISLLAVEHGIVSPR
                     DVATGMATGKRQHQAITLTKETDNTSPLFYQFISRNELIKTAEITFFGYGNPPGLSGG
                     REAI"
BASE COUNT          375 a          385 c          402 g          300 t
ORIGIN      
        1 tgtcgtgaag aacgaggatg ccaacaagca gagcaatgcg atcctcgatg atctgcaaaa
       61 ggatcccaac gccaacaggc tcaagcaggg tatcgagttg ctcgatcaat tgaagaaaga
      121 aggaaaaatc tcggaagccg atgcagatgc cgcgaagaac agcctgctga aaactttcca
      181 ggacagcagc aacgcaaaga cgccgatggg caacgaagaa atcggcaaac tggccgacaa
      241 gtccaaacag accaacaagg acatttctgt tactcgcccg ggtggcgaaa cggtcgaaat
      301 caaaaatccg gccaccgaag gttcattttc ctctgcatcc aacgcagttg gcacgaccct
      361 cgacatggtt gccggtggcg ggacgttgaa attccttcca acggggcaga ccgttgccgt
      421 tgatccaact cccgcgcaga tgaaaattat tgccgacaag gacggttggg tgccaaccaa
      481 gatgtccgat tatctgacgc tcgccgttgg aggcaggctg atcggactaa ggacattcac
      541 cggggttctg agatatctat ccctggttcc gcagggcagc gtacagacgc tcaacatggt
      601 gtgtcattgc ccggacaatt tgacgctcgc ttttcaacgc gatttcgtat tttcggagca
      661 gagcggcgta accaccattg gtccgccgga cccacaggac ggtcccttcg aggaagtcac
      721 aattccagaa ctcatgcaat tgaacgagtt ggactcgatt gatggtgact ccggcttgat
      781 aatcggactg gccgatgttc gtgcggcatt tgcggccaat gccgaactca ggctgttctg
      841 cattggtcgc ggagccaatg aacagatcgt gcagacgtta gccaacctcc tgcaagtcaa
      901 aataattgcc ttcaccgatg cagtaatcgc gttcagggtc gaggtgcttg aaggtgctac
      961 ggtcgggaac cgtcccgcgc tttcaaagcg aattttggcc acgcctcgct tcagcagcgg
     1021 ggcgcctttc gtttacaaca actttgaaca gatggtgagt gaagacgctg cgaagaccgg
     1081 aaagctcata atcctcccga gacgttcgtg aactgtgtac ccctaagacc agagattcac
     1141 tgcgatgctt tccgcatatc ttaaactcga gggtgacacg acgggaaaga tcagggggcc
     1201 cgttcgggac cgtgacagtg ataaagacgg cagcatttcg ctcctggcgg ttgagcatgg
     1261 cattgtttca cctcgggatg ttgccaccgg aatggccacg ggcaaaagac agcatcaggc
     1321 gatcacgctt acgaaagaga cagacaatac ttcgccgctt ttttatcaat tcatttcgcg
     1381 caacgaactg atcaagacgg cagagattac gttcttcggg tacggcaacc cgcctgggct
     1441 gtccggcggg agggaagcaa tc
//
LOCUS       DASTWE010000667         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1440182, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000667 DASTWE010000000
VERSION     DASTWE010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440182"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFT30_06910"
     CDS             <1..472
                     /locus_tag="VFT30_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092397.1"
                     /translation="VGPVMKINTPPWIEEPGARFRRVRVIGSLLSVLAVSALYADASA
                     LAQSVSGASSVRSFDGGIGNLFRLGGGSLYVDPQGTQGFLFSPGQSFVQSYNFRNPTT
                     GQAWSGAVMTFGPQLSIGLIQGANQVGSATVLPGPPRQSAPLPPIESTLLDQIP"
     gene            complement(531..>1281)
                     /locus_tag="VFT30_06915"
     CDS             complement(531..>1281)
                     /locus_tag="VFT30_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5092398.1"
                     /translation="QELLAGSRTDVPDRLRVFQERLNQTSVSQATRVDRFASLQWKTT
                     TEEGVLTNVIGMIPGTGTESVIIGAHRDHFGRPGGILFPGADDNASGTAVILQIARTL
                     TKIGLSPTRTILFVSFSGEERDLLGSRLYTSRPVVPLNSTKAMINIDHAGVGNGRLTV
                     GVTGLEKEVLLEVGKKTGVHEKLDLYGFFPGGDHVPFKEAGVPTVTVVSGGVHPHFHQ
                     PSDTVETIDPDILKTVTRYVLAVTWQLAYQP"
BASE COUNT          274 a          358 c          336 g          313 t
ORIGIN      
        1 ggtaggacct gtcatgaaga ttaatactcc tccttggatc gaagaaccag gcgctcgttt
       61 tagacgagta cgtgtgatcg gcagtctgct gtctgtcctg gcagtcagtg ccctttatgc
      121 cgacgcgtct gccttggcgc agagtgtatc gggtgcctcc agtgtgcgat catttgatgg
      181 agggattggg aatctgttca ggttaggcgg tgggagtctg tacgtagatc ctcagggaac
      241 acagggattt ctcttctccc ccggacagag ctttgtgcaa tcctataatt ttcgaaaccc
      301 taccacaggt caggcttgga gtggtgccgt catgaccttt ggcccgcaac tctccatagg
      361 gcttatccag ggtgcaaatc aagtcggatc cgcgaccgtc cttcccggac caccacgtca
      421 atcggctccc ttgcctccga ttgagtccac gttactggat cagattccct aacaggatag
      481 gaaaaagtcc tccaagctac gttcttcctt tttgactctg tcgcaaaacg tcacggttga
      541 taggcaagtt gccacgtcac ggccaacacg taccgagtca cagtttttaa gatgtcggga
      601 tcgattgtct cgacggtgtc gctcggttga tgaaagtgcg ggtgtacccc cccactcacg
      661 acggtgactg tcggcacgcc ggcttccttg aacggtacat gatctcctcc gggaaagaat
      721 ccatagagat ccagtttctc gtgaacgccg gttttcttcc ccacctcaag tagaacctcc
      781 ttttctaatc ccgtcacgcc gacggtgagc cgtccgtttc caactccggc atggtcgatg
      841 ttgatcatgg ctttcgtaga attgagtgga accacaggcc gcgatgtata aagacgcgaa
      901 ccgagcaaat cgcgctcctc gccgctgaag gagacgaaga ggatcgttcg ggtgggactc
      961 aagccaatct tcgtgagagt ccgtgctatc tgtaagatga ccgctgttcc ggaggcattg
     1021 tcatccgcgc cgggaaagag aattcctccc ggacgaccga agtgatcacg atgggctccg
     1081 atgatcacag actctgttcc ggttccagga atcatcccaa tcacattggt caagactcct
     1141 tcttccgtcg tcgtcttcca ctgaagcgat gcaaatcgat ccacccttgt tgcttgtgac
     1201 accgatgtct ggttcagacg ctcttgaaaa acacgcaggc gatcgggcac gtccgtacgc
     1261 gatcctgcga gaagctcctg a
//
LOCUS       DASTWE010000668         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1472415, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000668 DASTWE010000000
VERSION     DASTWE010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472415"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..>1042
                     /locus_tag="VFT30_06920"
     CDS             95..>1042
                     /locus_tag="VFT30_06920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5092399.1"
                     /translation="MPPSERAAYLSKVCAGDTSLRQAVESLLKAHDESRNLVDQPIHQ
                     VAEVLLEDHNFRPGDMVAHYRILSLLGEGGMGKVYLAEDTKLARKVSLKFISKTFAGD
                     HEQMRRFAHEARSASALNHPNIITIHEIGHDGDRQFIATEFIEGQTLRERLRSHLELD
                     EILEISIQVASALVAAHRSNIVHRDLKPENIMIRKDDGLVKVLDFGLAKISPNRPRDE
                     FKDPEAATMRIVNTAPGVIIGTVAYMSPEQSRGESVDERSDIWSLAVLIYEMVTGSSP
                     FIAASSHEIISAIRSKQPAPPMARFSANVPERLEEIVEKA"
BASE COUNT          263 a          279 c          268 g          232 t
ORIGIN      
        1 ctattaacgg gccgaacatc tgaccccgag ccttccggaa atgccagatt ctcactggga
       61 aaatctgaaa gacatatttc atgcagcagc atcattgccg ccaagtgaac gcgccgctta
      121 tctctccaaa gtctgcgctg gcgatacttc cctgcgacag gcagtagagt cactgcttaa
      181 ggcacacgac gaaagccgta acttggtgga tcaacctatc catcaagtag ctgaggtgct
      241 gctggaggat cacaacttca ggcctggcga catggtggcc cactatcgaa ttctgtcgct
      301 actgggtgag ggcggcatgg gcaaggttta cctggctgaa gacacaaagc ttgctcggaa
      361 ggtttcgctg aaattcatct cgaaaacctt cgcgggcgac cacgaacaaa tgcgacgttt
      421 tgcgcacgag gctcgttcgg cgtcagccct caatcacccg aacatcataa cgatccatga
      481 gatcggccac gacggtgatc ggcagtttat tgccactgaa ttcattgaag gtcagacgtt
      541 gcgcgagcga ttgcgctccc atctggaact ggacgaaatc ctggagattt caatccaggt
      601 agcttcagcg ctcgtcgctg cacacagatc aaatattgtc catcgtgatc tgaagcccga
      661 aaacatcatg atccgtaaag acgacggact cgtgaaagtt ctcgactttg gattggccaa
      721 gatatctccg aatagaccac gcgacgagtt caaagatcct gaagcggcaa cgatgcggat
      781 cgtgaacact gcacccggcg taatcatcgg aacagttgct tacatgtcgc ccgaacaatc
      841 acgcggtgag agtgtggatg agcgttcgga catctggagt ctggcggtgt tgatctatga
      901 gatggtcact ggctcgtcgc cattcatcgc tgcaagttca catgaaatca tttcagccat
      961 ccgctctaaa cagcccgcac ctccaatggc tcggttctca gctaatgttc ctgagcggct
     1021 cgaagagatc gttgaaaagg ct
//
LOCUS       DASTWE010000669         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_922952, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000669 DASTWE010000000
VERSION     DASTWE010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922952"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFT30_06925"
     CDS             complement(<1..462)
                     /locus_tag="VFT30_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092400.1"
                     /translation="MNETRLNLASKSNWQRITPDDLRCLAAGVLRVPIEEVRWFYHDE
                     DLAIDPKGMATIRQRKDALYVLDDGGFEKSRFMSCMGAMHWDQIDFLPVVELFKSLLP
                     GTGSAVFELIRGLYDDQNKGHSAPRALRYRGIPTYPSEAAFRLFSGFFTPQP"
     gene            complement(756..>1512)
                     /locus_tag="VFT30_06930"
     CDS             complement(756..>1512)
                     /locus_tag="VFT30_06930"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU5092401.1"
                     /translation="SEKAGVPRGAITFVDRADREVVPVLLKQDRFIDLIIPRGGESLM
                     KLIAEYSTIPVVKHDAGVCHVYVDADANSAMAEAICVNAKAQRPSTCNAMETLLVHQS
                     VARTLLPKLAQSLSMANVEIRGCPKTCQLIPEVKPASEQDYGKEFLDLILAVKIVKNM
                     DEAMEHIAQYGSRHTEAIVTSDYGRAMRFLKEVDASAVLVNASTRLNDGYQFGLGAEI
                     GISTSRIHARGPMGLDELTCSKFIVLGSGQLRE"
BASE COUNT          357 a          436 c          393 g          326 t
ORIGIN      
        1 cggctgtggc gtgaaaaatc cgctgaacag gcgaaaggct gcttcggatg gatatgtagg
       61 aatcccgcga taccgtaagg ccctcggagc agagtgtccc ttgttttgat cgtcatacaa
      121 tccacgaatc agctcaaata cggcagagcc tgtccccgga agcaaggatt tgaacaactc
      181 caccaccggg agaaaatcaa tttggtccca atgcatggca cccatgcagg acatgaagcg
      241 actcttttca aatcctccgt catctagaac atacaacgcg tcttttcgct gtcgaatcgt
      301 ggccatccct ttgggatcga tagcaagatc ctcgtcgtga taaaaccacc gcacctcctc
      361 gattggcact cgtaacaccc cggcagccag gcagcgaaga tcatccggcg tgatcctttg
      421 ccaattggac ttcgaggcta aattcagcct agtttcattc accagcccac cgggcttgat
      481 cccgacccac cgtccccaat caagtcggac tcgcgcccgc ttcagagaga caaccccggc
      541 accggtcgac tcccattcac actcatgtag gggatgacct acagggtctg tctcaagaaa
      601 gcgtcgtcca tcgggccgat aaaaaacgag gtgtccgttc ggaagagttt caacatgtcc
      661 gccggccttg tcgaaggact cagcaagtgt acgggtggaa ggaaaccgga gatggccggg
      721 agtgctcagg gcaaatgcaa tggggtcggg ggggttcact cgcggagttg gccgctcccc
      781 aaaacaatga atttggagca ggttaactcg tctaacccca tggggcctcg tgcatgaatg
      841 cgcgaggtgc tgataccgat ttcggctccg agaccgaact gatacccatc gttgaggcgg
      901 gtggaggcat tcacaagaac agcactggca tcgacctctt tgagaaaccg catcgcacgt
      961 ccgtaatctg acgtcacgat agcttccgtg tgtcgagatc catattgcgc aatgtgttcc
     1021 atcgcttcat ccatattctt cacgattttc acggccagaa tcaagtcaag gaactctttg
     1081 ccgtaatcct gttcgctggc cggcttgacc tcaggaatca attggcaggt ctttggacag
     1141 ccacgaatct cgacgttggc catgctgagg ctttgggcaa gcttgggcag cagagtccgc
     1201 gcgaccgact gatggaccag cagggtttcc attgcgttgc aagtggacgg ccgctgcgct
     1261 ttggcattga cacaaatggc ctccgccatt gccgaattcg cgtcagcatc tacatagaca
     1321 tggcacacgc ctgcatcatg cttcacgacc ggaatcgtcg agtattccgc gatgagtttc
     1381 atcagcgatt caccaccgcg cggaatgatc aagtcgatga accgatcttg cttaagcaac
     1441 accggcacca cctcacgatc tgcacggtca acgaacgtga tcgcaccgcg tggaactccg
     1501 gctttttccg at
//
LOCUS       DASTWE010000670         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_599417, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000670 DASTWE010000000
VERSION     DASTWE010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599417"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFT30_06935"
     CDS             complement(<1..369)
                     /locus_tag="VFT30_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092402.1"
                     /translation="MRFILSVPLIAVVMVVYTALAINPEQFSAGSAFMDWVLPSGAEF
                     FLTLGDIFVILGLVALFFEVIKSARLGPGTIVDHMLSTATFVIALILFLLVQLFGTPA
                     FFMLTLMALVDVVAGYTISIF"
     gene            516..>1019
                     /locus_tag="VFT30_06940"
     CDS             516..>1019
                     /locus_tag="VFT30_06940"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU5092403.1"
                     /translation="MALQLGDVAPDFEAETTEGHIKFHDYIGNGWVVFFSHPKDFTPV
                     CTTELGAMARMKHEFDKRNTKLIGISVDSVEDHQRWKNDIKEVSGAAVNYPMIGDPDL
                     SVAKLYGMIHPNATGNAKGRTAADNATVRNVFVIGPDKKIKLIIAYPMTTGRNFDEVL
                     RVVDSLQL"
BASE COUNT          265 a          279 c          289 g          186 t
ORIGIN      
        1 gaagatcgag atcgtatagc ccgccacgac atcgaccagc gccatgagag tgagcatgaa
       61 gaaggcgggc gtgccgaaga gttgcaccag gaggaacagg atgagcgcga tgacaaaggt
      121 cgcggtcgag agcatgtgat cgacgatcgt gccggggccg agccttgccg actttatgac
      181 ctcgaagaat agcgccacaa gccccaggat cacgaagatg tcgcccaagg tcaggaagaa
      241 ttcggcacct gacggcagca cccagtccat gaaggcagag ccggcagaga attgctcggg
      301 attgatggcg agcgccgtat agacgaccat gacaacggcg atcagaggaa cggagaggat
      361 gaagcgcatg agagatcctt tttccttcgg caagttgaac tgatttgcag aaggaagaaa
      421 cccatcttat tttactgacg cagggtcccc atattgttct ttattccgaa cgatcgttcg
      481 cgtatataga cgaaaaatca gcaacggagg tcagaatggc acttcaactt ggcgacgtag
      541 cgcctgattt cgaggccgaa actaccgaag gccacatcaa attccacgat tacatcggca
      601 atggctgggt cgtcttcttc tcgcatccca aagatttcac gccggtgtgc acgaccgagc
      661 tgggcgccat ggcgcgcatg aagcatgaat tcgacaagcg caacaccaag ctcatcggca
      721 tctcagtcga ttcagtcgag gaccatcagc gctggaagaa cgacatcaag gaagtgagcg
      781 gcgctgccgt gaactatccg atgatcggcg atcccgatct cagcgttgcc aagctctatg
      841 gcatgatcca ccccaatgcg accggcaatg ccaagggccg cacggccgcc gataatgcga
      901 cagtgcgcaa tgtcttcgtc atcgggcccg acaagaagat caagctcatc atcgcctacc
      961 cgatgaccac cggccggaac tttgacgagg tgctccgggt cgtcgattcg cttcagctt
//
LOCUS       DASTWE010000671         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_18220, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000671 DASTWE010000000
VERSION     DASTWE010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18220"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFT30_06945"
     CDS             <1..377
                     /locus_tag="VFT30_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEU5092404.1"
                     /translation="VPFDRVVLDLPEPWQVVPHAIKALRSGGIYLSFVPTVPQVMQTV
                     EALERTAVFGMIETFETLLRTWSVQGRSVRPDHRMVAHSGFITVARKVEPGLLGPIPE
                     RGIFADEDQEGAPDEAEEELSL"
     gene            374..>1025
                     /locus_tag="VFT30_06950"
     CDS             374..>1025
                     /locus_tag="VFT30_06950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5092405.1"
                     /translation="MNRLEGKVAVVTGGNAGIGEAIAKRFAEEGASVVITGRRQQELD
                     RVASVIRLNKGKVLGIAGSVTDETHVQDVVRRALDSFGRIDVLVNNAGIGEFGKRLHE
                     TDDVIWSRVLDINVTGVFRITRAVIPHMLKQGRGSIVNISSIASLVGLSSLAAYTASK
                     GALDALTRALAVEYAKEGIRCNVVNPGLIDTPMAAPLIANPDMLQPILAQYAIRRPG"
BASE COUNT          235 a          244 c          306 g          240 t
ORIGIN      
        1 aagtgccttt cgatcgcgtc gtgcttgacc tgccggaacc ctggcaggtc gtaccacatg
       61 ccattaaagc ccttcgatcc ggcgggatct atctgagctt tgttcccaca gtacctcaag
      121 tcatgcaaac ggtggaggcg cttgaacgca cagctgtatt tggaatgatt gaaacgtttg
      181 agacgttgct gagaacctgg tccgtgcagg gcagaagtgt gcggcctgat catcgcatgg
      241 tggctcattc tgggtttatc acggtggctc gtaaggtcga accaggactc cttggaccaa
      301 tccctgagag aggaattttt gccgatgagg accaagaagg cgcacctgat gaagcagaag
      361 aggaattgag tttatgaata ggttggaagg caaggtagcc gtggttacgg gaggcaatgc
      421 tgggatcggc gaagcgatcg cgaaacgttt tgccgaggag ggagcatcag ttgtcatcac
      481 gggacgtcgg caacaggaat tggatcgtgt ggcgagtgta atccgactca ataaggggaa
      541 ggtactcgga attgctggct cagtgactga tgaaactcat gtgcaggatg tcgtccgtcg
      601 agccctcgat agttttgggc ggatcgacgt tctggtcaac aatgcgggaa tcggtgaatt
      661 tggaaaacgg ctccacgaaa ccgatgatgt tatctggtcg cgtgtgctcg acatcaacgt
      721 gaccggggta ttccgcataa cacgagccgt cattccccac atgctgaagc aaggacgagg
      781 ttcgatcgtc aatatctcgt caattgccag tttagtaggt ctttccagct tagccgcgta
      841 tacggcatcc aaaggtgcgc ttgacgccct gacgcgtgcg ctggcggtcg aatatgcgaa
      901 ggaaggaatt cgctgcaatg tggtgaatcc tggtctgatt gatacgccta tggcggctcc
      961 cctgatagcc aatcccgaca tgctccagcc aattcttgcc caatatgcga ttcgtcgtcc
     1021 tgggc
//
LOCUS       DASTWE010000672         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_405809, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000672 DASTWE010000000
VERSION     DASTWE010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405809"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFT30_06955"
     CDS             <1..989
                     /locus_tag="VFT30_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU5092406.1"
                     /translation="DMVLSASDYPDYFPQEFNLDYNLETKILVVDYVLPAPENIPTVR
                     EVKFVPSRDEFVEQYLSQVQVSKLYDDLLYQIVLRTIHELFEADTIGAIGIVVFNGIV
                     TSVDRSTGKKVTACVLSLQARRDEFMAVDLANVEPKACFKALKGVGSSKLHSLAAVPP
                     IMQIRRDDGRFISAYEVANTLDESSNLAAMDWQDFEHLIREVFEKEFSTTGGEVKVTQ
                     ASRDGGVDAIAFDPDPIRGGKIVIQAKRYTHTVGVGAVRDLYGTVMNEGANKGILVTT
                     SDYGPDAYSFANGKPLILLNGANLLHMLEKHGHRARIDLQEARRIAMSQPVR"
     gene            1509..>1736
                     /locus_tag="VFT30_06960"
     CDS             1509..>1736
                     /locus_tag="VFT30_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092407.1"
                     /translation="MDHTTEQMVRSCVLELGVLSAEIQILKPTHLVFYTASLYPGLLS
                     NLAVDGQKRWRDLKRETVKCGQKNLLWWDRET"
BASE COUNT          433 a          426 c          484 g          393 t
ORIGIN      
        1 gcgatatggt gctctctgcg tcagattacc ctgactattt cccacaagag tttaacctcg
       61 attacaacct ggaaaccaag attcttgtcg tcgactacgt gctgcctgct cccgagaaca
      121 tcccgacggt gcgagaggtg aaattcgttc caagtcggga cgagtttgtg gaacagtatc
      181 tatctcaggt acaagtatcg aagctctacg atgacctgct ttatcagatc gtgctgcgca
      241 cgatccatga actgttcgag gcagacacaa ttggcgcaat cggcattgta gtatttaacg
      301 gcatcgttac gtccgttgac cggagcaccg gcaagaaggt aactgcgtgt gttctatcgt
      361 tacaggcacg acgcgatgag tttatggcag tcgacctcgc taacgtcgaa ccaaaagcgt
      421 gtttcaaggc actcaagggc gtcggcagct ccaagctgca cagcttggcg gcagtcccac
      481 cgatcatgca aatccggcgt gacgacggtc ggttcatttc ggcctatgag gtggcaaaca
      541 ccctcgatga atcttctaat ttagccgcca tggattggca ggacttcgag catctcatcc
      601 gtgaggtgtt tgagaaggag ttttctacaa ctggaggtga ggtgaaagtc actcaggcaa
      661 gccgcgacgg cggtgtggac gccatagcgt tcgatcccga tccgatccga ggcggaaaga
      721 tcgtcatcca agcgaagcgg tacactcaca ccgtcggcgt tggggcagtg cgggatctct
      781 acggcactgt gatgaacgag ggtgcaaata aaggaattct tgtcaccacg tccgactacg
      841 gacctgatgc ttactccttc gcaaacggga agccgctgat attgcttaac ggagccaacc
      901 tgctccatat gcttgagaaa catggccaca gggcgcgtat tgatttacaa gaggctcgtc
      961 gaatcgccat gagtcagccg gtccgatgat tatcgttaag tctgcgaggt tagttctcca
     1021 gccgccgggc ctaacgaccg gctggaggcg caccggcggt gaggcgacgt gtcctgaccg
     1081 aacgctaatg gccgccggcc gctcagccgg agcattagac ggaagaaatg gaacaaaagc
     1141 aattcgatga actttgcacg ttatatgaga gcagccttag gctcctgcgc aatcccacct
     1201 gtgagaaatg tgaagcgaag gttcggaacc cgctttcagt gtggcatgta ggaaagagct
     1261 ttcaaaagag tgcgattcga gtgttttttg tgggcaagcc ccatcgaggc actcctggct
     1321 tcattcggcc gtcaggattg atcgatcctc gaaagagggt cgaggaattg aaagaaattt
     1381 catgggctta ttggagctat actcgagaga tcggaaagaa attatttcca gacgaaaacg
     1441 atggctggaa ctgtattgcc ttaaccaatt tagtgaaatg cacaagcgta agccgtgatg
     1501 actcatcggt ggaccatacg acagagcaaa tggtccgatc ctgtgtgctc gagttggggg
     1561 tgctgtcggc tgaaattcaa attctgaagc caacacatct tgtattctac acagccagtc
     1621 tatatccagg gcttctgtcc aatctggccg ttgacggtca gaagcgatgg cgagacttga
     1681 agcgagaaac tgtaaagtgt ggtcagaaga acctgctctg gtgggaccgc gaaacc
//
LOCUS       DASTWE010000673         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1084682, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000673 DASTWE010000000
VERSION     DASTWE010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084682"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFT30_06965"
     CDS             complement(<1..204)
                     /locus_tag="VFT30_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5092408.1"
                     /translation="MTASKIPSQVVFVIDDDASMRDAVSRLLNAVGLTVQTFASAREF
                     LAGRLPDVPGCVVLDVRLPGLSGL"
     gene            complement(201..>1241)
                     /locus_tag="VFT30_06970"
     CDS             complement(201..>1241)
                     /locus_tag="VFT30_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092409.1"
                     /translation="ISDNFFGLDKEGRFTFFNKHAAKQMRTLGRDPDALIGRVAWEEF
                     PDMPNKENVLRVLSEHVAIVDEVYYAPLEEWVENHMYPSSDGGLVSFQKYITERKRTE
                     EELRKTQDELAHVTRVTTLGELTVSIAHEVNQPLGAIVTNGHACLRLLSRTNPDIDEA
                     RRAVECMIADGIRASEVIKRIRGLLKKSRDGKSSCKINDNIREVLALTAGELTKNGIN
                     VRTELAHGLPYVIADRVQLQQVVLNLILNSKEAMSGADWQPRELLIRSEQAGPEIVVT
                     VTDTGVGIAPENRERIFVPFFTSKEGGLGFGLSISQTIVEAHGGRLWTKPGHNGQGTT
                     FQFSLPTGENLR"
BASE COUNT          277 a          350 c          297 g          317 t
ORIGIN      
        1 gagaccgctc aagccaggta gcctgacgtc caaaactaca catcccggaa catcaggtaa
       61 cctccctgcg agaaactccc tggcggaagc aaacgtctgt accgttagcc cgaccgcgtt
      121 caataaacgg ctaaccgcgt cgcgcatcga cgcatcatca tcaatgacga atacaacctg
      181 actcggaatc ttgctggcgg tcatcgaaga ttttcacctg tgggcaaact aaactggaaa
      241 gtcgtcccct gaccattatg accaggcttg gtccatagtc tgccgccatg ggcctcaaca
      301 atcgtctggc tgattgatag tccaaatccc aaacctcctt ccttgctggt gaagaaagga
      361 acaaatatcc tttcccgatt ttcaggggcg atgccaacgc cggtgtctgt tacggtcacc
      421 actatctcag gtccggcttg ttcggatctg atcagtaatt cgcggggctg ccaatccgcg
      481 ccgctcatcg cctccttact attgagaatc agattcagca ctacttgttg gagctgcacc
      541 cgatccgcga tcacgtaggg caggccatga gccaattcgg ttcgcacgtt aattccgttt
      601 ttggttagtt ctccggctgt gagagcaagc acctcccgaa tattgtcatt gattttgcac
      661 gaggattttc catctcttga ctttttaagc aacccccgaa ttcgcttgat cacctcgctc
      721 gcgcgaatgc catcggcgat catgcactcg acagctcgtc gggcttcgtc gatgtcggga
      781 ttagtgcggg agagcagacg caaacaggca tgaccgttgg ttacaatggc gccgagcggt
      841 tggttgactt catgcgcgat tgaaacggtc agctctccca gagtggtaac gcgggtaacg
      901 tgtgccagtt catcctgtgt ctttcgaagt tcttcctctg ttcgtttacg ctcagtgatg
      961 tacttttgaa agctgactag tccaccgtcg ctgctcggat acatgtgatt ctcaacccac
     1021 tcttccaaag gcgcgtaata gacttcatcg acgatcgcaa cgtgctcaga caggacgcgc
     1081 aatacgtttt ccttattagg catgtccgga aactcctccc atgctactct tccgatgaga
     1141 gcatcaggat ctctgcccag ggttctcatt tgcttagccg catgtttgtt gaaaaatgtg
     1201 aatcgtcctt ccttgtccag gccgaagaag ttgtccgaga t
//
LOCUS       DASTWE010000674         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_696990, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000674 DASTWE010000000
VERSION     DASTWE010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696990"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1562)
                     /locus_tag="VFT30_06975"
     CDS             complement(<1..>1562)
                     /locus_tag="VFT30_06975"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5092410.1"
                     /translation="PSIGLLFPVTTRAQTFSDSGFTAEVVTTLPQFLPVGVTWASDGR
                     MFIWQRNGVIRIYKNGQLLPTPFLDISANVNTFDDRGMLGLALHPNFAVNGYVYVIYV
                     REDGGNPNDPSPKIERLSRFIANAQNPDVALPNSETILMTISTDFHHHENGTLRFGSD
                     GRLFVGHGEDATADGANGNAFVAQNLNDPRGKILRITEDGNAPGDNPFDDGTNSIRSK
                     VYSYGLRNPYRFTLHPVSGEPYLGDVGWNTWEEFDRGRGKNFGWPCYEGVNPQPEYQN
                     AFPTQCAPLTSGVVTAPLISYPHPGTPGAAGAPFVGSTAIGGPFYTATLYPEVYRNTW
                     FITDYAQGWIRRMIFDQNGDMTGTVPFATGLSGPVDLELGPDGMLYYVEIITGRVVRI
                     RYNGPSAVASVTPSSGYSPLTVNFSSTGSSSPNGTPLAYLWEFGDGQTSNAVNPSHTY
                     VAATVQTFTAKLTVTTNTNQSSSASIKVTVGSSPPTATIQSPINGTGVLPGQTIVYQG
                     SANDPDNGTIPS"
BASE COUNT          343 a          382 c          451 g          386 t
ORIGIN      
        1 tcgagggaat ggtcccatta tctgggtcgt ttgcagatcc ctgatacacg atagtttgcc
       61 caggcaagac acctgtgcca ttgatgggtg attggatggt tgccgtggga ggtgagctgc
      121 ctacggtgac tttgatgctc gcggaggaac tctgattcgt attggtggtg accgtcagct
      181 tagccgtgaa ggtttggacc gtagctgcaa cgtacgtatg agatggattg actgcattcg
      241 aagtctgacc gtctccgaac tcccacagat aagccaatgg agtgccattc gggctgctcg
      301 atccggtact ggagaaattg accgtgaggg gagagtatcc ggatgagggc gtgacggacg
      361 ccaccgcgga tggcccgtta taacgaatcc taacaacacg gcctgtgatg atctctacat
      421 agtacagcat tccgtcgggt cccagctcca agtcgacagg gcctgagagc ccggtggcaa
      481 aagggaccgt accggtcatg tcgccgtttt gatcgaagat catccgcctg atccagcctt
      541 gagcatagtc cgtaatgaac catgtgtttc gatacacttc cggatagagt gtggctgtat
      601 agaacggtcc accaatggcc gtacttccga cgaacggtgc cccggccgct cctggagttc
      661 cagggtgtgg atacgaaatg agcggtgcgg ttacaacacc gcttgtcagt ggtgcacatt
      721 gggtgggaaa agcgttttgg tattccggct gagggttgac accttcataa caaggccagc
      781 caaaattttt tccacgtcct cggtcaaatt cttcccaggt gttccacccc acatctccta
      841 aataaggttc ccctgagact ggatggagag taaagcgata gggatttcga aggccatagg
      901 agtagacttt cgacctgatt gaattggtgc cgtcgtcgaa gggattatcg ccaggagcgt
      961 ttccatcttc agtgatgcgt aagatttttc cacgcgggtc gttgaggttt tgcgcgacaa
     1021 atgcatttcc atttgcgccg tcagccgtgg cgtcctctcc atgccctaca aagagcctcc
     1081 cgtctgagcc aaatcgtaag gtgccgttct catggtggtg gaagtcggtg gaaatcgtca
     1141 tgaggatggt ttcactgtta ggcagtgcga cgtcgggatt ttgggcattc gcgataaatc
     1201 gggaaagtct ttcgattttt gggcttgggt cgttgggatt gccgccatct tcgcgtacat
     1261 aaatcacata gacatatccg ttcacggcga agttcggatg taacgccaag ccaagcattc
     1321 ctcggtcgtc aaatgtattg acattcgcgc tgatgtcaag aaacggtgtg ggcaataact
     1381 ggccattctt gtatatcctg atcacgccgt ttcgttgcca aatgaacatg cgcccgtcgc
     1441 tggcccatgt caccccgacc ggcaggaact gagggagtgt cgtgacgact tccgctgtga
     1501 atccagagtc actaaacgtc tgggcccgtg tggtgacagg gaatagtagt ccgatgctag
     1561 gg
//
LOCUS       DASTWE010000675         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1601958, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000675 DASTWE010000000
VERSION     DASTWE010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601958"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1744
                     /gene="hrpB"
                     /locus_tag="VFT30_06980"
     CDS             <1..1744
                     /gene="hrpB"
                     /locus_tag="VFT30_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEU5092411.1"
                     /translation="KVVLATSIAETSLTIDGIRVVIDAGRVRIPRYDPRSDLTRLETV
                     RVTKDSAEQRRGRAGRLEAGMCYRLWTEKEQAMLAPRRPPEILEADLTPLMLDLAQWG
                     VRDPAELSWLTPPPAGAIVPARELLIQLGAFSADGLPTDHARRMAELPLHPRLAHMML
                     RAVPLGFADLACEVAALLSERDILHGPRERQNADLRVRLDVLRGQYDSVGLEVNRAAV
                     DRVRRTAQLWRRRLISGQSREAIDNRCDPSSSVGWLIALAYPDRIARRQSDREGRYRL
                     VNGRGARFKTADPLAVEPFLVIADLDAGDQWSEINLAAPIAQKDIESLYHDQLVEEEE
                     VAWDEAFNAVRSLRRQRLGAMILLEEAASSPDANTVKEALLQGIYKAGLEVISFDHLL
                     QQWRARVMWLRRIDDPRSGWPDLSDEALLQTLDQWLGPYLTGITTLERVKRLDLTIPL
                     HALLTHEQWRLLDRLAPTHVTVPSGSRLPIDYEQPESPVLAVRLQEMFGCKDTPCVAD
                     GKIPLILHLLSPAKRPVQVTQDLGGFWKRAYQDVRKELRGRYPKHHWPEDPIGAAPTA
                     KAKQRNRSRPSGRP"
BASE COUNT          489 a          611 c          624 g          495 t
ORIGIN      
        1 aaaagtcgtg ctcgccacct ccatcgccga aaccagcctg acgatcgacg gcatccgcgt
       61 ggtgatcgat gcaggccgag tacgtattcc acggtacgat cctcgctctg atctgactcg
      121 attggaaact gttcgcgtca caaaagattc tgcggaacaa cgccgcggca gggccggacg
      181 actcgaagcg ggcatgtgtt accggctgtg gacggaaaaa gaacaggcta tgcttgctcc
      241 ccgtcgtcca ccggaaattc tcgaggcgga tctcactcct ctgatgctcg atcttgccca
      301 gtggggcgta cgtgatccgg cggaactgtc atggctcact ccaccacctg cgggtgcaat
      361 cgttccggcg agagagttat tgatccaact cggagccttc tcagctgatg gcctgccgac
      421 tgaccatgcc cgacgaatgg ccgaacttcc actgcatccc cgcctggcgc acatgatgct
      481 tcgggcagtg ccattgggtt ttgccgatct tgcttgcgag gtggcggcgc tgttgagtga
      541 acgagacatt ctccatggac cacgtgaacg gcagaatgca gatttgcgag tcaggctgga
      601 cgttctgcgg gggcagtacg attccgtcgg tctggaggtg aatcgagctg cagttgaccg
      661 agtgaggcga acggctcagc tatggcggag acggctcatc agtggacaat ctcgcgaggc
      721 aatagacaat cgatgtgatc cttcaagctc agtcgggtgg ctcatcgcac ttgcctaccc
      781 tgatcgaatt gctcgacgac agtcggatcg tgaagggcgt taccgccttg taaatggccg
      841 aggcgctcgg tttaagacag ccgatccctt ggccgtagaa cccttcctcg tgatcgccga
      901 ccttgatgcc ggcgatcagt ggtccgagat caatcttgcg gctccaatcg ctcaaaagga
      961 tattgaatcg ctttaccatg accagttagt cgaggaagaa gaggtggcct gggatgaggc
     1021 attcaacgcg gttcgatccc tgcgccgcca acgacttgga gcgatgattc ttttagagga
     1081 ggcagcctca tcgcctgatg caaacacggt aaaagaagca ctcttgcaag gtatctataa
     1141 agctggactt gaagttattt cattcgatca tctgctccaa caatggcgcg ctcgcgtgat
     1201 gtggcttagg cgtattgacg accctcgctc aggatggcct gatctttccg atgaagcatt
     1261 gctccagaca ttggatcagt ggcttggtcc ctacttgacc ggtatcacca ccctcgagcg
     1321 tgtgaaacgg ctggatctga caatcccact ccatgcctta ctgacgcacg aacaatggcg
     1381 tcttcttgac cgtttggctc cgacccacgt cactgttccg agtggctccc gccttcctat
     1441 tgattacgag caacccgagt ctccagtgtt ggcagtacgt ttgcaagaga tgtttggttg
     1501 caaggatact ccgtgcgtag cggacgggaa gattcctttg atcttgcatc tcctgtctcc
     1561 tgccaaacga ccggtgcagg tgacccaaga tcttggcggc ttttggaaga gggcctatca
     1621 agatgtccgc aaagaattac gtggccgata tcccaaacac cattggcctg aagatccgat
     1681 aggggccgcg ccaacggcca aggcaaagca acgaaaccga agccgaccgt cgggaagacc
     1741 ctgattctcc tactttgccg gcaggctgtt caaaaggcct cattctcacc cgccccccac
     1801 ccaggtgcgc cgagacgcat tccataggca aggcccagcg aggtccgcga cgcgaagaat
     1861 aacggcgcga tgaataaaga gccatgttgt gaaagtcccc aaatggtggg gcggctatgt
     1921 cttgcgagaa acgaagctgg aggacttttt cagcagcctg ctagggttct tccgtgtagc
     1981 ttgttctacg tgggaagggt acacatgaca caaccttatt cactccagaa aatacggaac
     2041 agatgccgtc acgtcaccgg ttagagaaaa agcttgctca tgaagtagat atattgggga
     2101 ttgccgttgc gctcgcattc ctcgtgatta ttagtgtgtt taccggaggc cggggcgatc
     2161 gcagtgtgag cgaggatcga cagtcttcgt cggcttccat gcagatcgca cctgccttg
//
LOCUS       DASTWE010000676         1879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_147663, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000676 DASTWE010000000
VERSION     DASTWE010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147663"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..863)
                     /gene="traT"
                     /locus_tag="VFT30_06985"
     CDS             complement(114..863)
                     /gene="traT"
                     /locus_tag="VFT30_06985"
                     /inference="COORDINATES: protein motif:HMM:NF017619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HEU5092412.1"
                     /translation="MHIVFRRVLSHSLPLALVTLLTLVTGCSNVIRSGLMNSNTIFLD
                     PNTNRNIYTQLRNVSENQQVTLNEVNTKLAAKGYQLVQEPEQANFWIQAKVIYCHKAA
                     DSVMPEGVAKSGPGAGISSGGTAMASTVSIGDETARGIAGMPDMGTIMRQAMAMSGGR
                     AGFGGMSDFPGMQPPPKEDGVTYLCITDVQISDRKIGKSIGQPAGGQPSAGSKVQQMR
                     MVGHVRQKSLDIPEATPIVQEKISTGIAGLF"
     gene            complement(863..1699)
                     /gene="traT"
                     /locus_tag="VFT30_06990"
     CDS             complement(863..1699)
                     /gene="traT"
                     /locus_tag="VFT30_06990"
                     /inference="COORDINATES: protein motif:HMM:NF017619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HEU5092413.1"
                     /translation="MAAMLADGGWFFKPIPVKTIHVQLSLAVAIETDSFVQSARFCRE
                     VSMRYLALCILFLLPFTAYAGNIKDTDLISSNTFFLPPSTHKTVFIQARNSSDNQDVS
                     FHDLGSRLVAKGYQVVNDPDSAQYIVLANIVYCNITKPEMPVEAMVASGYGSAFNTTL
                     GAMQGLASMASMAGPYGAMGGAAASMGLSAIQGIGDLIGSSHSAPKMPDDVNYACVAD
                     VQITDQGAAPLPGGMKTGTGAEPGVYQTRLAADVHQKKLDETEATPLLQRKLSAAVAG
                     NF"
BASE COUNT          392 a          534 c          512 g          441 t
ORIGIN      
        1 gcggcccaac tatgcataac acacctctac ggttcaggaa caaacgccat gacgtttgcc
       61 gtttcgttga gttgaagctg cgagatcatt tatggggctt tatccggacc ggtctagaac
      121 agccctgcaa ttccggtgga gattttctct tgcacgattg gagtggcttc aggaatgtcc
      181 aaactttttt gccgcacgtg gccgaccatt ctcatctgct gaaccttcga gccggctgac
      241 ggctgacctc cggccggctg accgatcgat ttgccaatct tccggtcgga aatttggacg
      301 tcagtaatgc acaagtaggt cacgccatct tccttgggtg gtggctgcat gccggggaaa
      361 tcggacatgc ctccaaatcc agctcgtcca ccactcatag ccatggcctg tctcatgatc
      421 gtgcccatgt ccggcatgcc ggcaattccc ctcgcagtct cgtcacctat ggatacggtc
      481 gaagccatcg cggtacctcc gctgctgata ccagcaccag ggccggattt ggctacgcct
      541 tccggcatca cactatcggc agccttgtga caatagatca cctttgcctg tatccagaag
      601 ttcgcctgct ccggctcctg aaccagttga tatccctttg cggcgagctt ggtgttcacc
      661 tcgttcaacg tcacctgttg attttcggac acgttcctaa gttgagtata gatgtttcgg
      721 ttggtgttgg gatccaggaa gatagtgttg ctgttcatga ggccggatcg gatcacgttg
      781 gaacagccgg taaccagtgt gagcagggtc accagcgcaa ggggaagaga gtgagagaga
      841 acacgtctga atacgatgtg cattaaaagt ttcccgccac cgcagcgctc agtttccgtt
      901 ggagcaacgg cgtcgcttcg gtttcatcca atttcttttg gtgcacatcg gccgccagcc
      961 tggtctggta cacgccgggt tccgcccctg ttcctgtttt cattccaccg gggagcggcg
     1021 cggcgccttg gtccgtgatc tgaacgtcgg ccacgcaggc atagttcaca tcatccggca
     1081 tcttgggcgc tgagtgagag gacccgatca gatcgccgat gccctgaatc gcgctcaaac
     1141 ccatggatgc cgccgcgccg cccatcgcgc catagggtcc cgccatactc gccatgctgg
     1201 caagaccttg catggcaccc agtgtcgtgt tgaaggcgga cccatagccg ctcgccacca
     1261 tcgcttcgac cggcatctcg ggtttcgtga tgttgcagta gacgatgttt gccaacacga
     1321 tgtactgcgc gctgtccgga tcgttcacga cttgataacc cttcgcgacc aagcgtgagc
     1381 cgaggtcatg aaacgagacg tcttgattgt cggacgaatt gcgagcctga atgaacaccg
     1441 tcttgtgtgt gctcggcggc aggaagaagg tgttgctgga gataagatcc gtgtctttga
     1501 tgttgccggc gtaagcagtg aacggcaata ggaagagaat gcaaagtgcc aggtatcgca
     1561 tcgacacctc acgacaaaac cgagcggatt gtacgaaaga gtcggtttca atagcaacgg
     1621 ccaaactgag ctgaacatgt atggttttca ctgggatggg cttgaagaac caccccccgt
     1681 cggctagcat ggcggccatt tcatccgttg gatggcccgc acaggaggcc gtagcgtgcc
     1741 gacccacttg ttcctatttt tcagtgttgt gctattggtc acaagttgca gcagtggaga
     1801 aaagcatttt ccatcctcga atccgccgga atatgacccc aagaaggtct acacagttcc
     1861 tgccacaccc acccgatcc
//
LOCUS       DASTWE010000677         1439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_793864, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000677 DASTWE010000000
VERSION     DASTWE010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793864"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFT30_06995"
     CDS             complement(<1..355)
                     /locus_tag="VFT30_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092414.1"
                     /translation="MTDRGQPHILLLQLEFPQWEQARAWTYSACYGVGEGLRAGGAKC
                     TTIPLLANAPYSADAWLAHARHAVAGKQFDQVWVWLVHSPLTPSVLDWISTLAPIRVG
                     IVMESLTYDETDYAWA"
     gene            complement(383..>1439)
                     /locus_tag="VFT30_07000"
     CDS             complement(383..>1439)
                     /locus_tag="VFT30_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5092415.1"
                     /translation="YKLGTLRGRLPFTSIQTMRGCPWRCIFCASEALKTTEMRVRSPR
                     SVVNEIKSVVEQFGTRHFYIVDDVMTLWQDHILEIAELIDQEGLRVTFEGSTRANLVE
                     DEVIARLVKSGLIRLSFGLETVDPEIRKTMKKQVPLEHYIRANGICNKYGVEALNSVM
                     IGLPGETRETVRATLRFLQNAREVKQANIAIAVPYPGTEFHELAMKGDKGVNLMTEDF
                     SEYRRYGSAVTTVGDLTPHDLIDLQNEGFVSIYSAPWRWIPMLQKHGIIGGSLMLLRV
                     ARLLWSKLWKRSSNSQSPHFISLGDGGFGRIQDPVMPMSEPAAPGASGSLLQIARLEK
                     ELVAPAGHYGTPGSPNA"
BASE COUNT          320 a          445 c          371 g          303 t
ORIGIN      
        1 ccgcccaggc atagtccgtc tcatcgtatg tcaacgattc catgacgatg ccgactcgga
       61 taggcgcgag agtcgagatc caatccaaca ccgacggggt gaggggtgaa tgaacaagcc
      121 atacccagac ttggtcaaac tgttttccgg ctaccgcgtg acgggcatga gcaagccagg
      181 cgtcagccga atagggcgca ttcgccagga gggggatcgt ggtgcacttc gcgccgccag
      241 ctcgtaaccc ttcccctacg ccatagcagg cggaatacgt ccatgcccgg gcctgttccc
      301 actggggaaa ttccaattgg agcaataaaa tatgtggctg tcctctgtcg gtcatgcgtt
      361 cacgcctatg tcaactcgac tgttacgcat tgggactgcc cggtgtgccg taatggccgg
      421 ccggagcaac cagttccttt tccagtcgtg cgatctgaag caacgatcct gacgcgccag
      481 gagccgcggg ttctgacatc ggcatcaccg ggtcctgaat acggccgaat ccgccgtcac
      541 cgaggctgat gaaatgcgga ctctggctgt tcgatgaacg cttccacaat ttgctccata
      601 agagcctcgc cacgcgcagc aacatcaacg aacccccgat aatgccgtgt ttctggagca
      661 tgggaatcca acgccagggc gccgagtaaa tactgacgaa tccctcgttc tgtagatcga
      721 tgaggtcatg aggcgtcaga tcgcccacgg ttgtcaccgc cgatccgtag cggcggtatt
      781 cggagaagtc ttcggtcatc agattcacac ccttatcgcc tttcatggcc aactcgtgaa
      841 actcggttcc cggatagggt accgcaatgg caatattggc ctgtttgacc tcacgagcgt
      901 tttgcaaaaa cctcaaggtg gctcggacgg tttcacgcgt ttctcccggc aaaccgatca
      961 tcaccgagtt caacgcctcc acgccatact tgttacaaat gccgttcgcc cgaatgtaat
     1021 gttccaacgg cacctgcttc ttcatcgtct tccgaatctc cggatcgaca gtctccaggc
     1081 cgaacgagag acggatcaag ccgctcttca caagccgggc aatcacctcg tcttccacaa
     1141 gatttgcccg cgtgctgcct tcgaacgtga cccgcaaccc ttcctggtcg ataagctccg
     1201 caatctccaa aatatggtct tgccaaagag tcatgacatc gtcgacaatg tagaagtgcc
     1261 gcgtgccgaa ttgctccacg acagacttta tctcgttgac caccgagcga ggcgaccgta
     1321 cccgcatttc agttgtcttc aacgcctccg acgcgcagaa aatgcacctc cagggacagc
     1381 ctcgcatcgt ctgaatcgag gtgaacggca gccgcccgcg caacgtccca agcttgtag
//
LOCUS       DASTWE010000678         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_212201, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000678 DASTWE010000000
VERSION     DASTWE010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212201"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFT30_07005"
     CDS             complement(<1..516)
                     /locus_tag="VFT30_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092416.1"
                     /translation="MLKGIHVSLLIGPAVPIPVSQEVLDALTNVQVISSTDDKMNGFE
                     LTFVLSTRSPLHTIFLLSAGSPIPLIRVVIAVVINGTTEVLMDGVMTQHEIKPGTDAA
                     HATLIVKGKDLTAVMDYLEFNIPYPAMPAEARVALMLAKYLPFGVIPLVIPSILIDVP
                     IPVERIPNQRGT"
     gene            complement(509..871)
                     /locus_tag="VFT30_07010"
     CDS             complement(509..871)
                     /locus_tag="VFT30_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU5092417.1"
                     /translation="MTDPLQLLLQAAPLNTVLFPPNSRYHGVPTAVHIMPDGTTVSYV
                     RRRFIPSPEQLTVLQEHTLVQGERLDHLAAKYFGDPELFWRLCDANVVMRPGDLTEEI
                     GRRIRITLPEGIRGVTGA"
     gene            complement(868..1521)
                     /locus_tag="VFT30_07015"
     CDS             complement(868..1521)
                     /locus_tag="VFT30_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092418.1"
                     /translation="MTTFPNSPKLLKGGLVLIDPETAMVQRIISLQYNPDSLSRSFQI
                     KGVGAESGDRSEALRLKGPPVETIKVEAELDATDQLEFAEAKATTVGIHPQLAALETI
                     VYPTSGQLLANNRLAQAGKLEITPMETPLTLFVWSKTRVVPVRLTEFSVTEEAFDPAL
                     NPIRAKVSLGMRVLTVDDVGFNHKAGTLFMSYLQVKEQLAARNQGGSLNVLGIGAIP"
     gene            complement(1648..>1867)
                     /locus_tag="VFT30_07020"
     CDS             complement(1648..>1867)
                     /locus_tag="VFT30_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092419.1"
                     /translation="PTASALVKGFASVGSSDEDKVRLAEEWAEIVQDKVIELTGDVIV
                     TNWRRFHRMGIPALTRNSVVITLDRPDL"
BASE COUNT          393 a          556 c          492 g          426 t
ORIGIN      
        1 ggttccccgc tgattcggta tccgttcaac gggaatcggc acgtcgatca gaatgctggg
       61 gatcaccagc ggaatgacgc cgaacggaag gtatttggcc agcatcaatg ccacccgtgc
      121 ttctgccggc atcgccgggt aagggatatt gaattccaga tagtccatga ccgccgtgag
      181 atccttgcct ttcacgatca aagtggcatg agcagcatcc gtcccgggct tgatctcgtg
      241 ctgcgtcatc accccatcca tcaacacctc cgtcgtcccg ttaatgacga ccgcaatgac
      301 gactcggatc aacggaatcg gcgaaccggc ggacagcagg aagatcgtgt gcaaaggcga
      361 gcgcgtgcta agtacaaagg taagctcgaa cccgttcatc ttgtcgtcgg tgctggagat
      421 cacctgcaca ttggtgaggg cgtccagcac ctcctgagag actgggatcg gcacggccgg
      481 tccgatcaac aaactgacgt gaattccctt aagcacccgt cactcctctt atcccttcag
      541 gaagcgtgat ccgaatccgt cggccgatct cctcagtcag atcccccggt cgcatgacca
      601 cgttcgcgtc gcaaagacgc caaaacaatt caggatcgcc gaaatacttc gccgccaggt
      661 gatccagccg ctccccctgc accagagtgt gttcttgcag caccgtcaat tgctcagggg
      721 atggtataaa ccgacgccgc acatagctca ccgtggtgcc atcaggcatg atatgcaccg
      781 ccgtgggcac accgtgatag cgactgttcg gcgggaaaag gacggtgttc agtggggccg
      841 cttgcagcaa caattgcaat ggatccgtca tgggattgct cctattccga gcacattcaa
      901 cgatccgcct tggttcctgg cagccagttg ctccttcacc tggagataac tcatgaacag
      961 cgtacccgcc ttatggttga agccgacgtc gtccaccgtc agcacgcgca tgcctaagct
     1021 gactttcgca cgaatgggat tgagcgccgg atcgaaggcc tcttcagtca cgctgaattc
     1081 cgtcagccgc accggcacca cgcgcgtttt gctccagaca aatagggtga gtggcgtttc
     1141 catcggcgtg atctccaact tgccggcttg ggccagacga ttattggcaa gcaattgacc
     1201 gctggtcggg tacacgatcg tctctagcgc agccagctgc ggatggatcc ccactgttgt
     1261 tgctttggcc tcggcaaact ccaactgatc ggttgcgtcg agttctgcct ccaccttgat
     1321 cgtctcaacc ggcggaccct tcaagcgcag cgcctccgat cgatcgccac tctccgctcc
     1381 aactcccttg atctggaacg atcggctcag cgagtcgggg ttgtattgca gcgaaatgat
     1441 acgttgcacc atggccgtct ccgggtcgat caagaccagg ccgcctttga gaagtttggg
     1501 agagttggga aatgtcgtca tcgaatattc gtgctattgc ttattgccta taaggcgcga
     1561 atatttggtt cgcttcgggc tttcattgtg catctcgcat cgcccccaaa tttcctcatt
     1621 cttggacttg agccattttg attgcattta caagtccggc cgatccagag tgatgactac
     1681 ggagttgcgg gttagggcgg gaatgcccat ccggtggaac cttctccaat ttgtgactat
     1741 cacatcacca gttaattcga tgaccttgtc ttgaactatc tcggcccatt cttccgcaag
     1801 acgaaccttg tcttcatccg aactgcccac gctggcgaaa cctttcacca aggcggatgc
     1861 cgtcggg
//
LOCUS       DASTWE010000679         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1375718, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000679 DASTWE010000000
VERSION     DASTWE010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375718"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFT30_07025"
     CDS             complement(<1..436)
                     /locus_tag="VFT30_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092420.1"
                     /translation="MLIIDRIVWFITSGILIFIVAVIALMIVRLITDAMDLNPFAWTS
                     RTVRRLSDGFVIPVRGGLRQLGMDPKFAPLAVILIVILLGYYLLALVRTIASTIVGVW
                     AGVQSGSGFAVLGHILYGLLSIYLLLIIMRIVFSWGRVSYRNR"
     gene            complement(441..>1171)
                     /locus_tag="VFT30_07030"
     CDS             complement(441..>1171)
                     /locus_tag="VFT30_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5092421.1"
                     /translation="LMVNVDPAQDPLAIRLKAIRARITAAAERCGRPAHEVRLIAISK
                     THPASVIKKVVEFGALDIGENRVQEAEEKIAEVGRDAVRWHLVGHLQANKARRAVNLF
                     DVIHSLDSLDLARRLDRLCVEEGREKLAVLIQVDLGQEETKSGIDESEITHLVEGLGP
                     LSRLELTGLMTLPPFFDDPEQSRPFFRRLRELRDELAARGAFASGKGELSMGMTNDFE
                     VAIEEGATMVRVGTAIFGERGSRH"
BASE COUNT          288 a          352 c          278 g          253 t
ORIGIN      
        1 cgcggtttct ataactaact cttccccatg agaatacgat tctcataata atgagcagca
       61 ggtagatcga taacagcccg tacaaaatat ggccaagcac tgcgaaccct gagccgcttt
      121 gcacacccgc ccacacgccc acaattgttg aagcgatagt tcggaccaac gccaataaat
      181 aatagccgag cagtatcact ataagaatga ccgctaatgg agcgaacttc ggatccatac
      241 cgagctggcg caatccgccg cgcacgggaa ttacaaatcc atcactgagt cgccgcactg
      301 tccgcgacgt ccacgcaaat ggatttaaat ccatcgcatc ggtgatcagt ctcacgatca
      361 tcagcgctat gacagcgacg atgaagatca agatgccaga agtaataaac caaactattc
      421 gatcgatgat gagcatgtta tcaatgcctc gaaccgcgtt ccccgaagat ggccgtgccg
      481 acgcgaacca tcgtggcgcc ctcttcaatt gctacttcaa aatcgttagt catccccatc
      541 gacagctcgc ccttcccact cgcaaatgcg ccacgggccg caagctcgtc tctcaactct
      601 cgtaatcgac gaaagaaagg ccggctttgt tccgggtcgt caaagaacgg cggcagcgtc
      661 atcagcccgg ttagctccag tcgactcagt ggtccaagac cttcaaccaa atgggttatt
      721 tctgattcgt cgatacccga tttggtttct tcctggccca gatcgacctg gatcaatact
      781 gctagcttct ccctgccctc ctcgacacac aaccgatcca gtcgccgggc taaatcgagc
      841 gaatccagcg agtgaataac gtcaaaaagg ttaacggcac gtcgtgcttt gttggcctgg
      901 aggtgcccga cgaggtgcca gcgcaccgca tcccgtccca cttccgcaat cttctcctcg
      961 gcctcctgga cacgattttc accgatatcc agtgccccaa attcgaccac tttctttata
     1021 acagaggccg gatgggtctt gctgatggcg atgagtctga cctcgtgggc aggccggccg
     1081 caccgttcgg cggcggctgt aatgcgggca cggatggcct taagtctaat agctagtgga
     1141 tcttgcgccg ggtcgacgtt aaccataagg g
//
LOCUS       DASTWE010000680         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1084870, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000680 DASTWE010000000
VERSION     DASTWE010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084870"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..634
                     /gene="ruvA"
                     /locus_tag="VFT30_07035"
     CDS             26..634
                     /gene="ruvA"
                     /locus_tag="VFT30_07035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5092422.1"
                     /translation="MIAFLTGRLAFKAPTHLTLDVQGVGYEVHIPLSTYYALPNLDEV
                     TALNIHTHLREDAIQLFGFLSQSEKESFLLLTSVSGIGPKLALSVLSSLSVTDLVHAI
                     QSEDVEKLATVPGIGKKSAGRIALELKDKVNKIHGVRPQATAADAFEPDGPYEDALSA
                     LVNLGYRAQDAKEALQRVTKAATGSLALKELIRKGLKELARG"
     gene            621..1127
                     /locus_tag="VFT30_07040"
     CDS             621..1127
                     /locus_tag="VFT30_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092423.1"
                     /translation="MQGGKLMPLDVRNIGKILLYSIAIGVVLVVAFPSRSIAQEATEP
                     KSIRMTCGACPDGYATTGVTSSLEICKDGDPTLVQCVPVGANMLGVCGSCPDGYAEIG
                     SSSVPARCGNKDGGRLTQCQLRKMEHNFPDSTQGYKRCPPDCGSMAQPGQGALPPPPK
                     YQQMPESK"
BASE COUNT          290 a          332 c          366 g          279 t
ORIGIN      
        1 tcttaccctc ggataacgaa acgacatgat cgcctttctc acggggcggt tggcattcaa
       61 ggctcccact cacctcacgc ttgatgtgca gggggtcggg tacgaagttc atattccact
      121 cagcacctat tacgccctac cgaatctcga cgaagttacc gcgctgaata ttcatacgca
      181 tctccgtgag gatgccattc agctcttcgg atttctctcg caaagcgaga aggaatcatt
      241 tttgctgctg accagcgtgt cgggcatcgg cccgaagctc gccctcagtg tgctttcgag
      301 cctgtcggtc acggatcttg ttcatgctat ccagtctgag gatgtcgaaa agcttgccac
      361 cgttcccggg atcgggaaaa aatcggcggg gcgtattgcg cttgaactga aagacaaagt
      421 gaataaaatc catggtgttc gtccccaagc cactgccgct gatgcgtttg agccggatgg
      481 cccttacgag gatgcactct ccgctctggt aaacttgggt tatcgtgccc aggatgccaa
      541 ggaagccttg cagcgggtga caaaggccgc gactggatcg ttggcgttga aagagctcat
      601 ccgcaaaggg cttaaggagc ttgcaagggg gtaagcttat gccgctcgac gtcaggaaca
      661 tcggcaagat tctcttgtac agcattgcta tcggggttgt tctcgtagtc gcgttccctt
      721 ctcggagtat cgcgcaggag gccacggaac cgaaaagtat cagaatgacc tgcggtgcgt
      781 gtccggatgg gtatgcgacg accggcgtca cctcgtctct ggaaatctgc aaagacggag
      841 atccgactct cgtgcaatgt gtgccggtgg gggcaaacat gctgggcgta tgcggatcct
      901 gcccagacgg ctatgccgaa atcggaagct cctcagttcc tgctcggtgt gggaacaagg
      961 atggtggccg gctgacgcaa tgtcagttga ggaaaatgga acacaatttc cccgactcga
     1021 ctcagggata taaaagatgt cctcctgatt gcggcagtat ggctcagcct gggcaagggg
     1081 ctctgccgcc tcctccgaag tatcaacaga tgccggagag caagtagaca tgggcgtcgc
     1141 accatgacgg aacggtttgt caccaatcgt gccacggacg aagagcgggg tcaggagaat
     1201 gtgcttcgac ctcagacgct caatgaatac gtcggccaag acaaaatgaa ggaatcgctt
     1261 cgaattt
//
LOCUS       DASTWE010000681         1293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1020421, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000681 DASTWE010000000
VERSION     DASTWE010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020421"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..769)
                     /locus_tag="VFT30_07045"
     CDS             complement(86..769)
                     /locus_tag="VFT30_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092424.1"
                     /translation="MPGYGIFFGAHPLTAIATLTNQLASILAPFHPLLVYVDSDVGVA
                     LNRAIAQRGSAWFERMVQRMNGWNLLLYTDKPFSDQQSVVAFDQQAHQFALRLLDHWH
                     SLKFMLNTDDTELGQLKTQLLGYFQLNELAVYTGVDPDRLPSYCGTYAARDPAVTEPA
                     VEIKCVAGQLVINRYWPNGTALVAEDVYQFRLENTNYRVIFEPQANGGIIALTLLYRG
                     QRYQLHKID"
     gene            complement(797..1033)
                     /locus_tag="VFT30_07050"
     CDS             complement(797..1033)
                     /locus_tag="VFT30_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092425.1"
                     /translation="MHHKLILFSGSGPGAGKSTLSAFLAQQFTAHAIPTRWIYEEDTL
                     DAFTPFFQALRQKQLNTLDLFLDATRSLVIECQT"
BASE COUNT          308 a          325 c          366 g          294 t
ORIGIN      
        1 gggattgcca tcaataaacc acaagaaact atgcgtatcc cgtaggagct tcatggcata
       61 tactcgcgga aatcatgaag gcgtctcagt cgattttatg aagctggtac ctttggcctc
      121 gatacagaag cgttaacgct attatccctc cgttggcctg tggttcaaag atgacacggt
      181 agttcgtgtt ctcgagccga aactggtaga cgtcttcggc tactagcgcc gtgccgttcg
      241 gccagtagcg attgatgacc aactgcccag ccacacactt gatctcgacc gctggctcgg
      301 tcactgccgg atcacgcgcg gcatatgttc cacaataact tggtagccga tccggatcaa
      361 cgccggtata tacagccagc tcatttagtt ggaagtagcc gaggagttgt gttttgagtt
      421 ggccgagctc cgtatcgtcg gtgttgagca tgaacttgag gctgtgccag tgatcgagca
      481 ggcgcagggc gaattgatgc gcctgctgat caaacgcgac cacactttgt tgatcgctga
      541 agggcttgtc ggtgtagagc agcaagttcc agccattcat ccgctggacc atgcgctcga
      601 accaagcgct gccgcgctga gcaatcgccc gattgagcgc aacaccaaca tcgctatcca
      661 catagacgag cagcggatgg aatggggcaa ggatgctggc aagttgattc gtcaaggtag
      721 cgatggccgt aagcggatgt gcgccaaaga aaatgccgta gcctggcaac agtgaatcga
      781 cgatactgat ggtcggctat gtctgacatt ctatgaccag gctacgggtg gcatccagaa
      841 atagatcaag ggtgttgagc tgcttctgcc tcagcgcctg gaagaagggt gtgaaggcgt
      901 caagggtgtc ctcttcgtaa atccatcgag ttgggatggc gtgggcagtg aactgttgtg
      961 ccagaaaggc ggaaagcgtg gatttgccgg caccaggacc ggaaccacta aacagaatca
     1021 acttatggtg catcacacca ccttgtcggc gagggctgcg aaaccgtgca tcgattatac
     1081 tatcagatga ggcgcgatca tgtagtatgg cattgaatgg tggcggccca acttcgttta
     1141 agacagaatc ctatggagaa tatagcgcag ctattcctgg agaagccgtc tccgtgcttc
     1201 tgcgagtgat acgccgccct ccgattgcat ccgctgccac gaatattgca tcaattctag
     1261 aaagtcgacg ttctcaccga gcttccaggt tgc
//
LOCUS       DASTWE010000682         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_568131, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000682 DASTWE010000000
VERSION     DASTWE010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568131"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..>1020)
                     /locus_tag="VFT30_07055"
     CDS             complement(90..>1020)
                     /locus_tag="VFT30_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092426.1"
                     /translation="QIWHLSYPGGEARKITNDLNGYTSISLNADSSVLVTIQENHATD
                     IWVAPDGDAGRANKFTSVSSNMDGLDSVRWTPNSKIVFHSLAGARDGIWIMEADGKNR
                     KQLTTSETVDYNPSVTTDGRHVVFVSERTGAPAGWIMDIDGSNQKKLNLSGVNPQAAD
                     GWVVYPKEQSLWKSPIDGGEAVRLSEEQLIRCGVSQDGKMVACSLEAPGSPIRLAVLP
                     IDGGSPAKIFDAKFHFPARMRWTPDGRAVTYIGRENGLADIWSQSIDGGEPKRLTNFK
                     ADNIFSFDWSRDNRLVISHGTSTSDVVFMRNEH"
BASE COUNT          199 a          287 c          280 g          254 t
ORIGIN      
        1 ttgtgtgcgg gaattacttt ggcaagacgt tagttgatgt gttacgtccg ccgggattgt
       61 gttaatggct gcctgttcgg ccgggactat cagtgctcgt tccgcataaa gacaacgtcg
      121 gatgttgaag tgccgtgtga gatgacgagt ctgttgtcgc gcgaccaatc gaaggagaat
      181 atgttatcgg ctttgaagtt cgtcagcctc ttcggttcac cgccgtcaat cgattgactc
      241 cagatgtcag caagaccgtt ctcgcgaccg atataagtaa ctgcccgacc gtcgggcgtc
      301 caccgcatac gtgcggggaa gtgaaattta gcgtcgaaaa tcttcgcggg cgaaccgcca
      361 tcgatcggca gaacggccag cctgataggc gagcctggcg cttccagcga acaggcgacc
      421 atctttccgt cctgtgagac gccgcaccgt atgagctgtt cttcactgag tcgcacagcc
      481 tcgccgccgt caatcggtga cttccacaga gattgttctt tcgggtagac cacccacccg
      541 tcggccgcct gcggattgac tcccgagagg ttcagttttt tttgattgct cccgtcaatg
      601 tccattatcc atccggccgg agcgcccgtg cgctccgaaa cgaaaacgac gtggcgacca
      661 tctgtcgtga ccgaggggtt atagtccacg gtctcggagg tggttaattg ttttcgattc
      721 tttccatccg cttccatgat ccagattccg tctctggcac ccgccaacga gtggaaaacg
      781 attttgctgt tgggcgtcca cctcacgctg tccaacccgt ccatgttact ggagacggat
      841 gtaaacttat tggcgcggcc tgcgtcgccg tccggcgcga cccagatatc cgtagcgtga
      901 ttttcctgta tcgtcaccag aacactggag tccgcattga ggctaatgct cgtgtaacca
      961 ttaaggtcgt tcgtgatttt tcgcgcctcc ccgcccgggt aagagagatg ccatatctgg
//
LOCUS       DASTWE010000683         1518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1181762, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000683 DASTWE010000000
VERSION     DASTWE010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181762"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFT30_07060"
     CDS             complement(<1..485)
                     /locus_tag="VFT30_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU5092427.1"
                     /translation="MIAVVLLIVIESPSIVEAASKPSPELARHLAEIARLARPSPMMT
                     VPAGTFLLGSNRVDNDPYGGRTQFDDTELPQHQVWLDTYEMDRDEVSLGEYLAFLQKQ
                     KVHPSDELQKLLWHVITVHSISDQTLTRWPALYVTWAEANNLCAAKHARLPTEAEWEK
                     AA"
     gene            701..>1518
                     /locus_tag="VFT30_07065"
     CDS             701..>1518
                     /locus_tag="VFT30_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU5092428.1"
                     /translation="MSTQYLFAAHYLRLFVFGVWIITMMLPLPLNAETQPLSQDAFLR
                     ARLVTVGILADTQDQRMPEKPGKVVVRGTGFHLRDGYVITARHAAEKHELSTGVVIQK
                     QIRVLTNDLHELPADLVGDSAFMDVVVYRVAEQHRSKLQAWTSFATAEASPGMEVFTV
                     GYPLGWGPTMAFGKIGNTNTFLQTVDTRLIQADLSTCSGNSGGGLFNAQGEIVGIVHA
                     VIQTDKEETQAHCSRMTFIIPGILSERIINAALSGQPLAFSRLGVHMTSVKDGT"
BASE COUNT          348 a          408 c          412 g          350 t
ORIGIN      
        1 gccgccttct cccattcagc ctcggtcggt aatctggcgt gcttggccgc gcatagattg
       61 ttcgcctctg cccatgtgac atagagcgcg ggccagcggg tcagggtctg gtcggagatg
      121 gaatggacgg tgatgacgtg ccagaggagt ttttgcaact cgtccgaggg atgaaccttc
      181 tgtttctgca gaaaggccag atattctcca aggctcactt catctcgatc catctcatag
      241 gtatccaacc agacttgatg ttgaggcagt tctgtatcgt caaactgagt ccggcccccg
      301 taggggtcat tatcgactct gttgcttccg agcaaaaacg taccggccgg gacggtcatc
      361 atcggcgatg gtctggccag cctcgcgatc tccgctaaat ggcgggccaa ttccggggat
      421 ggttttgagg cggcctctac gatggagggg ctttcaatga cgatgaggag aactaccgcg
      481 atcacaggcc atgcacaaca acgcggcata aaatcttgtc cgggagaacc atgcctggat
      541 cagatcgggt tgcactataa gccggatgtc tgcgacttac aatagtttcg gactgacgat
      601 caaaccgttg tgggtcattc ggcactcttt ccgaaacaac acgatccgat tatagtatgc
      661 agccctgaca cgtgtgcacc acgttgaaaa ggagcattga atgagcacgc agtacctctt
      721 tgcagctcac tacctgcgtc ttttcgtgtt cggtgtgtgg atcatcacga tgatgcttcc
      781 cctcccactc aatgctgaaa cgcagccgct cagccaagac gcatttcttc gggcgagact
      841 ggtgaccgtg ggcatcctcg cggatacaca agatcagcga atgccggaaa agcctgggaa
      901 ggttgtcgtc cgaggaactg gatttcactt acgagacggc tatgtgatta ccgcaagaca
      961 tgccgcagag aaacacgagc tttcgacagg tgtcgtcatt cagaaacaga tacgcgtgct
     1021 cacgaatgat ctccatgaac ttcctgccga tctcgtgggc gacagtgcgt tcatggatgt
     1081 tgtcgtgtac cgagttgccg aacagcatcg ctccaaattg caggcgtgga catcctttgc
     1141 cacggctgag gcatcacccg ggatggaagt ctttacagtc ggctatccac ttggttgggg
     1201 tccgaccatg gcttttggga agatcggaaa taccaatacg ttcctacaaa ctgtcgatac
     1261 ccgtctcatc caggccgacc tttcgacctg cagcggaaac tcgggaggtg ggctgttcaa
     1321 cgcacaagga gaaattgtcg gcatcgtgca tgccgtgatc caaaccgata aagaggagac
     1381 ccaggctcat tgcagtcgca tgacctttat cattccagga attctctcgg agcgcatcat
     1441 caacgcagcg ctttcaggac agcctcttgc cttctccaga cttggggtcc acatgacttc
     1501 tgtaaaagac gggacgaa
//
LOCUS       DASTWE010000684         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1569517, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000684 DASTWE010000000
VERSION     DASTWE010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569517"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFT30_07070"
     CDS             complement(<1..278)
                     /locus_tag="VFT30_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092429.1"
                     /translation="MKSLLRVLLYLRPHRALAIATLVSAACATAMELVPPWVIKVIID
                     DVIQAKQASLLPWAIGLLVGAHMLKNVFASLRIRLNNQLEQTVVHDLRR"
     gene            complement(301..846)
                     /gene="def"
                     /locus_tag="VFT30_07075"
     CDS             complement(301..846)
                     /gene="def"
                     /locus_tag="VFT30_07075"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5092430.1"
                     /translation="MKLKSGTMLKIAKLGNPILRKIAAPIDPREIKSSEIQRLIDDMF
                     ETMYDEPGIGLAAPQVSRSIQLVVMGCKGEGGFPETVLINPSIVYYGPEQVENWEGCL
                     SVDGLRGKVTRPSLVRVKGLDRKGKPLDFEATELYAVCIQHELDHLIGKVFLDRMTDM
                     STLTQLDEFAQYWQKEPTNVI"
     gene            complement(904..1692)
                     /locus_tag="VFT30_07080"
     CDS             complement(904..1692)
                     /locus_tag="VFT30_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5092431.1"
                     /translation="MINYASIAAIILVGLVKAQTLHALSDQPERTWPIQIPYEAPPKS
                     QDVPDVSVPPNKNPLSPEELKRTEALLPLLEGKQEFWAMGEFVHLGEPAIPILVQALA
                     MPSPRIRYNAIETLLMMKGVAGVPALVSTAKEAGEIPRVREHALRVAVRLDSTKAPEA
                     IEVMAKDPNPSIRKAAAFEARYVRQKAVIPILIPMVSDDERFVALSALQSLWVLTRHE
                     TEFHDWDTSTKQDRAAWSSEWTEWWDANKELFEIPEPPRPKRSS"
BASE COUNT          425 a          558 c          511 g          436 t
ORIGIN      
        1 cggcgaagat cgtggaccac agtctgctca agctgattat tcagacggat tctgagcgag
       61 gcaaacacgt tcttgagcat atgagcgccc accagaagac cgatcgccca cggcagcaat
      121 gacgcctgct tcgcctgaat cacatcgtcg atgatgacct tgatgaccca gggaggcacg
      181 agctccatcg ctgtcgcaca cgcggcgctg accagtgtcg cgatcgcaag agcgcgatgg
      241 ggccgaagat acaggaggac gcgaagaagg gacttcacaa caggctcgac aaggcaggca
      301 tcaaattacg ttcgtgggct ccttctgcca atactgcgca aattcatcaa gctgagtgag
      361 ggtggacata tcggtcatgc gatcgagaaa aactttgccg atcaagtggt cgagttcgtg
      421 ctgaatacag accgcgtaca gttctgtcgc ttcgaaatcg agaggtttgc cctttcgatc
      481 cagccccttg acccgcacca gcgagggtct ggtcactttg cccctcaatc catcgacgct
      541 cagacaacct tcccagtttt ccacctgttc gggcccgtaa tacacgatcg aagggttgat
      601 gaggactgtt tcgggaaatc cgccttcgcc tttgcaaccc atcacaacca gttgaatcga
      661 acgcgagact tggggagcgg ccagtccgat gcccggttcg tcatacatcg tttcaaacat
      721 gtcgtcgatc agccgctgga tctcggacga tttgatctca cgcggatcga tcggagcggc
      781 aattttgcga aggatagggt tacccagctt ggcgatcttc agcatggttc cgctcttgag
      841 tttcatatcg taattattcg taacacatga atcttcgggc ccgcaagctc ccccggattg
      901 ccgttacgaa ctccgcttcg gccgtggcgg ttcgggaatc tcaaagagct ccttgttggc
      961 gtcccaccat tccgtccatt cactcgacca cgccgcacga tcctgcttgg tcgatgtatc
     1021 ccaatcgtgg aactcggtct catggcgagt cagaacccac agtgattgta gtgccgacag
     1081 cgccacgaag cgctcgtcgt cgctcaccat cggaatcaag atggggatga cggccttctg
     1141 ccggacatac cgcgcttcga aagccgcagc tttcctgatg gatggatttg gatcttttgc
     1201 catgacttca atcgcctcgg gcgctttcgt cgaatccagg cgaacggcaa cgcgcagcgc
     1261 atgttccctc acccgaggga tttcgccagc ctcctttgcc gtggagacga gggcgggtac
     1321 cccggcaact cccttcatca tcagtaaagt ttcgattgcg ttgtaccgaa tccgcgggct
     1381 aggcatggcc agtgcctgaa caaggatagg aatagccggc tctcctaaat gcacgaattc
     1441 tcccattgcc cagaattcct gttttccttc gagcaacggc agcaaggctt ccgtgcgctt
     1501 gagctcttca gggctgaggg ggttcttatt ggggggaacc gagacatccg gaacatcctg
     1561 actcttcggc ggcgcttcat aggggatttg aatcggccat gtccgctcag gctgatcgga
     1621 caacgcatgg agcgtctgcg cttttactag accaacgaga ataatcgcgg caatacttgc
     1681 atagttgatc acgacgtgtg aactcctctt agtccagatc atcgatgcga cacattttag
     1741 aagagattga cacgagcctg tatccggcat cggctggagc gaggggtgca accgacagtc
     1801 gtcggcctta gtccaaccct gaattggctt ctcgcagatg cttcctcacc cgaactttct
     1861 catgatgctt ccgaagcaac tgccgtcgga ccacatcctg gttctctgcg acaatacgaa
     1921 ccaatcgatc
//
LOCUS       DASTWE010000685         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1375768, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000685 DASTWE010000000
VERSION     DASTWE010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375768"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..>1046
                     /locus_tag="VFT30_07085"
     CDS             93..>1046
                     /locus_tag="VFT30_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092432.1"
                     /translation="MPMGSPDRTSLLNSVRPLCPDIPSDVLQDFFARMDQEYFRRFEP
                     QTIAEHVRLTAQLTPERPCEIAFAEQQNKRFEITIVAYDYFSEFATICGLLSAFGLNI
                     EEGHIYTFADKIAQQSARASWTGYGPRVRAKGTPGLSRKKIVDMFRVLPVPGSELGMT
                     QQKQLSDALRSVITLLDTGQFNEARFAVNRRLVEQLGKRRGSFSGLLHPVQITFDNSQ
                     SPADTVMDIRSDDTPAFLYAFANALAMRNIYISKAQFDVEDGKIHDRFYVRNRHGQKL
                     TDATDQQQLRLTAVLIKQFTHALTWAPDPTKALEAFDQFLDL"
BASE COUNT          235 a          320 c          262 g          229 t
ORIGIN      
        1 atgtaggcgg tacagaggaa cgactcatgg cgcggcatta tactgacacg ttcctctacg
       61 cacaatgaaa ccgttcgggt atactgatgc ctatgccgat gggctctcct gatcgcacat
      121 cgcttctcaa ctcggttcgt ccgctctgtc ctgacattcc atccgatgtg cttcaagatt
      181 ttttcgcccg catggaccag gagtacttcc gacgattcga gcctcagacg attgcggaac
      241 acgtgcggtt aacggctcaa ctgacgccgg agcgtccctg tgaaattgcc tttgccgaac
      301 agcaaaacaa gcggtttgaa attacgatcg tggcctacga ttacttctct gaattcgcca
      361 cgatttgcgg cctgctctcg gcgttcggcc tcaacattga agaaggacat atctacacat
      421 ttgccgacaa gatcgctcag cagtccgccc gggccagttg gacaggatac ggaccgcgtg
      481 tccgcgcaaa gggcactccc ggtctgagca gaaaaaaaat cgtggacatg ttccgcgtcc
      541 tgccggttcc gggctcagag ttgggcatga cgcaacagaa gcaattgtcg gacgcactcc
      601 gctcggtgat cacactgctc gacaccggac aattcaacga ggcacggttt gcagtcaatc
      661 ggcgcctggt cgaacagctc ggcaagcgac gggggtcctt cagcggtctg ctccatccgg
      721 tccagatcac gttcgataac agccaatccc ctgccgatac ggtcatggac atccgatcgg
      781 acgatactcc ggcttttttg tatgccttcg ccaacgccct tgccatgcgc aatatctata
      841 tttccaaagc gcaattcgat gtcgaagacg gcaaaattca tgaccgcttt tatgtccgca
      901 accgccatgg ccagaaactc accgatgcga ccgatcagca gcagttgcgc ctcacggctg
      961 tgctcatcaa gcagttcaca catgccctca cctgggcccc cgacccaacc aaggccttgg
     1021 aagccttcga ccagttcctg gatctg
//
LOCUS       DASTWE010000686         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_891261, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000686 DASTWE010000000
VERSION     DASTWE010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891261"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..865)
                     /locus_tag="VFT30_07090"
     CDS             complement(149..865)
                     /locus_tag="VFT30_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092433.1"
                     /translation="MREGLLGGLKVRFVGGSDGRGGGDGPVVLLLHGFGAPGDDLVPL
                     ADVIEVPRGTRWLFPEAPLSLNMGFGDSRAWWIIDFARIQEDRAAGRIRDLSVEIPQG
                     LALARERFLAFLKELPRQFQIDYKKTVIGGFSQGAMLTCDAVLHTDYPFAGLVQLSGN
                     LLAQSVWGPLMPTRKGLPVFQSHGTLDDILPHIGAERLCEALTQSGLAVERHSFRGGH
                     EIPEPVLQRLTGFITRAFKA"
     gene            complement(940..2415)
                     /locus_tag="VFT30_07095"
     CDS             complement(940..2415)
                     /locus_tag="VFT30_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEU5092434.1"
                     /translation="MTRRTLETLSFDNTYARLPPAFYARLNPTPFNAPPYLVHANRAA
                     AELIDLDPAQLTRSEFTALFGGSALAPGMEPLAMLYAGHQFGVYVPQLGDGRAILLGE
                     VKNGKGERWDLHLKGAGMTPFSRDGDGRAVLRSTIREYLCCAAMQGLGIPTTYSLCLV
                     GSDDKVYREQIETGAMLVRMAPSHVRFGTFEIFYYRKQHEHLRTLADYVIDQHFPHLR
                     DVGEKYARFFTEVVERTAKLIAQWQAVGWAHGVMNTDNMSILGLTLDYGPYGFMDDYD
                     AGFICNHSDHNGRYAFNQQPYIGLWNLSCLAQALLPLAEKDALKAGLETYQPLFEREY
                     LRLMRTKFGLVGEQAEDDELIRDFLGLLQGSHADYTIVFRELSTFSVADGATNEKLRE
                     HFLNHGRFEEWAVRYRDRLRTECSCDDERRGRMNRVNPKYVLRNYLAQTAIEKAQNKD
                     FSEIDRLFTLLQDPFTEQPGMDAYALPPPNWGKHLAVSCSS"
     gene            complement(2429..>2641)
                     /locus_tag="VFT30_07100"
     CDS             complement(2429..>2641)
                     /locus_tag="VFT30_07100"
                     /inference="COORDINATES: protein motif:HMM:NF021568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HEU5092435.1"
                     /translation="ERQLAKTTEGIEMVKQLRQNLIAQGRDKLCEQVGEITGAKILGL
                     FTDIDVQLGERVFVFTMDRDIQNGSR"
BASE COUNT          594 a          772 c          706 g          569 t
ORIGIN      
        1 tgacaccatt gcgccaacga cttactcttg ttaccccatc gcttggacag aaaaccggtg
       61 atgaagagga tttgccggac tgatccctct gcacgatcgc cctttagacg tttgccgtcc
      121 gcacctggga gatcgaatgg ctgcatgttc atgctttgaa tgcccttgtg atgaaccccg
      181 ttagtcgctg cagcacgggt tctggaattt cgtgtccgcc tcgaaaacta tgccgttcta
      241 cggcgaggcc cgattgagtc agtgcttcgc acagacgctc cgcaccgata tgcggcagga
      301 tgtcatccag cgtgccgtga ctttggaaga ccggcagacc tttgcgtgtt ggcatgagtg
      361 ggccccagac cgattgagct agaagattgc cagagagctg cacgagaccg gcaaatggat
      421 aatctgtatg taagactgca tcgcaggtaa gcattgcccc ttgtgaaaac ccaccgatga
      481 ccgtcttctt gtagtcgatc tggaactgtc ggggtaattc cttgagaaat gccagaaaac
      541 gttctcgtgc cagagccaag ccctgaggaa tttccacgga cagatcgcga atgcggccgg
      601 cggcacgatc ttcctgaatt ctcgcgaagt cgatgatcca ccaggcacgt gaatcgccga
      661 aacccatatt gagcgagaga ggagcttcgg gaaacaacca tctggttcct ctaggaacct
      721 cgataacgtc cgcaagcggc accagatcgt cacccggtgc gccgaagcca tggagcagca
      781 ggacaactgg gccgtctcca ccaccccgtc catctgaacc accgacaaat cggaccttca
      841 atccacccag taagccttct cgcatgagaa ctccctaagg caccttggtt ttttggcttc
      901 tatctcccct gatcagggcg ccatgattct ctagtgccct tacgatgaac aacttacggc
      961 gagatgcttg ccccagttcg gtggaggaag cgcataggcg tccatgccgg gctgctcggt
     1021 gaagggatct tgaagcaaag tgaagagccg gtcgatttcg gaaaagtctt tattctgagc
     1081 cttctcaatc gccgtctgtg caagatagtt gcgcagcaca tacttgggat tcacacgatt
     1141 catccggcca cgccgctcat cgtcgcaact gcattcagtg cgcagccgat cccgatagcg
     1201 caccgcccat tcctcgaaac ggccatggtt gagaaagtgt tcgcgcaact tttcattggt
     1261 tgcgccgtca gccacggaga acgtactcaa ctccctaaac acaatcgtat aatccgcatg
     1321 gctgccctga agaaggccca agaaatcacg aatgagctcg tcgtcttccg cctgctcacc
     1381 caccaatccg aatttcgtcc gcatgagcct caaatactcc cgctcgaata agggttgata
     1441 ggtctcaagg cctgccttca atgcatcctt ttccgccaac ggcaacaagg cttgcgccaa
     1501 gcagctcaaa ttccagagcc cgatataagg ctgctggttg aaagcatagc ggccgttgtg
     1561 gtccgagtgg ttgcagatga agccggcgtc gtagtcgtcc atgaagccgt acggaccata
     1621 gtccagtgtc aggcccaaaa tcgacatgtt gtcggtgttc atgacgccgt gcgcccaacc
     1681 cacggcctgc cattgggcaa tcaacttcgc ggtgcgttcg acgacttccg taaagaatct
     1741 ggcgtacttt tctccgacat cacgaagatg cgggaagtgc tgatcgatca cgtagtcggc
     1801 cagagttcgg aggtgctcat gctgtttccg gtaatagaag atttcgaacg tgccgaagcg
     1861 gacatgggag ggcgccatgc ggaccagcat cgcgccggtc tcgatctgct cccgatacac
     1921 cttgtcgtca ctgccgacga ggcagagcga atatgtggta gggattccca agccttgcat
     1981 cgcggcgcaa cagagatact cacggatcgt cgagcgcagc accgcccggc cgtctccatc
     2041 gcgagaaaac ggcgtcatcc ctgcgccttt gagatgcaag tcccaccgct ctcccttccc
     2101 gttcttcacc tcaccgagca gaatcgcacg gccgtcgccg agttgcggaa catagacgcc
     2161 gaattgatgg ccggcgtaca gcatcgcgag cggttccatc cctggcgcca atgcactccc
     2221 gccgaaaagc gccgtgaact ccgaccgcgt aagttgcgcc gggtcaagat cgatcagctc
     2281 cgctgcagcg cgattagcat gaaccagata gggaggggcg ttaaacggag tcggattcaa
     2341 tcgcgcgtaa aaggcggggg gaagacgagc ataggtattg tcgaaggaga gcgtttccag
     2401 tgtgcgccga gtcatggaag actatatctt atcggctgcc gttctggata tcccgatcca
     2461 tcgtaaagac aaagactcgc tctccgagct gcacatcgat atccgtgaag agccccaaga
     2521 ttttggcacc ggtgatctcg ccgacttgtt cgcagagttt gtcgcggccc tgcgcgatta
     2581 aattttgccg gagctgcttg accatctcaa ttccttcagt cgttttcgcc agctgccgtt
     2641 c
//
LOCUS       DASTWE010000687         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_471020, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000687 DASTWE010000000
VERSION     DASTWE010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471020"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /gene="scpB"
                     /locus_tag="VFT30_07105"
     CDS             complement(<1..588)
                     /gene="scpB"
                     /locus_tag="VFT30_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5092436.1"
                     /translation="MTPLTVDVVHHGLEADAMNIGEVNGSPHDQSFDDNPVHGMGELQ
                     AILEALLFVSSEPLSLARLVAVIGSVPKVEVEEALRNIGQALEEEGRGVRLTVVAGGY
                     RLVTKQDYAPWIKRLDKVKTAAKLSRSALESLAIIAYKQPLVRSEIEEIRGVETSGVL
                     RTLLERKLVRIVGRKEVPGRPIMYGTTKFFLEHFGL"
     gene            complement(585..>1295)
                     /locus_tag="VFT30_07110"
     CDS             complement(585..>1295)
                     /locus_tag="VFT30_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU5092437.1"
                     /translation="IKKNEINIYDIPVAMIAQQYLHYLEAMEELNLNVAGDFLVMAAT
                     LLQIKSKMLLPADETADDDEEGPDPREELVRRLLEYKAFKEAARQLDDQEKVWREVFW
                     REQAPLVEEVEEDLPLENVSLFDLVDALKEVLERNPSSRLIEIVPDNLTVRERMNLIL
                     ETLEGKDSVSFAALFEGSSHRMVVVVTFLALLELMKLRVARVFQAETFGPILVSRMFS
                     LVPDPAELDDVDAEWRGA"
BASE COUNT          291 a          408 c          294 g          302 t
ORIGIN      
        1 caagccaaaa tgttcaagaa agaattttgt cgtcccgtac atgattggac ggccgggaac
       61 ttctttacgt ccgacgatcc tcaccagttt tcgttccaac agcgtacgca acacaccgga
      121 ggtttccacc ccacggattt cttcgatctc cgatcgcacc agcggttgtt tgtaggcaat
      181 gatcgccaac gattctagag cggatctgga cagttttgcc gccgtcttga ccttgtccag
      241 ccgcttgatc cagggggcat agtcctgctt cgtgacgagg cggtacccgc ccgcgacaac
      301 agtcagtcgc accccgcgtc cttcttcctc gagcgcttga ccgatatttc gcaatgcctc
      361 ttccacttca accttcggca cgctccctat cacggccaca aggcgcgcca aggacagcgg
      421 ttctgaagaa acgaagagca gtgcttccag aattgcctgg agctcgccca tgccgtgcac
      481 gggattgtca tcgaagctct gatcatgagg cgatccattc acctctccaa tattcatcgc
      541 gtcggcttcc agcccatgat gaaccacatc caccgtcagt ggagtcatgc tcccctccat
      601 tctgcatcga catcatcaag ctctgccgga tcgggcacta aagaaaacat ccgtgagacc
      661 aaaatcggtc cgaatgtttc tgcttggaat acacgggcaa cccgcaattt catcaattca
      721 agtaacgcca agaacgtcac gacgaccacc attcgatggc ttgatccctc gaacagcgca
      781 gcaaacgaga ccgagtcttt gccttcgagc gtttccagaa tgagattcat ccgctcgcga
      841 accgtgagat tgtcgggaac gatctcgatg agccggctgc ttggattccg ctccagcact
      901 tccttgagcg catccaccag atcaaacaac gagacattct ctaacggaag atcctcctca
      961 acctcttcga ccaatggggc ttgctcacgc caaaagacct cgcgccatac cttttcctgg
     1021 tcatcgagct gacgtgccgc ctctttgaaa gccttgtatt ccaacaaccg tcgaacgagc
     1081 tcttcccgcg ggtccggccc ctcttcgtcg tcatccgccg tctcatccgc cggcaacagc
     1141 attttggatt tgatctgcaa taacgtcgcc gccatgacca aaaaatcccc cgccacgttg
     1201 aggttgagtt cttccatcgc ctccagatag tggaggtatt gctgagcgat catcgcgacc
     1261 gggatatcgt agatgttgat ctcattcttc ttgat
//
LOCUS       DASTWE010000688         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_471077, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000688 DASTWE010000000
VERSION     DASTWE010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471077"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFT30_07115"
     CDS             <1..605
                     /locus_tag="VFT30_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5092438.1"
                     /translation="PGRVVVLGAGIVGSSATRIAVGMGAQVTVINLDIERLRYLDDQY
                     QGRIVTRAVSPATIEEAVCSADLVIGAVLVPGAKSPKLVSRSLVSRMKPGSVIVDVSV
                     DQGGCVETTKPTTHSKPVYVVDGVVHYCVANMPGIVPRTSTYALTNATLPYLLRLASD
                     GVTRAIQSDPGLAKGINLKDGKLTHRSVAEAHGLPFTPVL"
     gene            642..718
                     /locus_tag="VFT30_07120"
     tRNA            642..718
                     /locus_tag="VFT30_07120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:676..678,aa:Pro,seq:cgg)
     gene            1086..1382
                     /locus_tag="VFT30_07125"
     CDS             1086..1382
                     /locus_tag="VFT30_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU5092439.1"
                     /translation="MTQSTDEPSVRAHILLGGRVQGVGFRAFAARTATRLDLVGGVRN
                     LDDGRVELDVEGKRMVIEALLRELKIGPPTSRVTKIEMEWSTTTGRYSNFSIWY"
BASE COUNT          332 a          388 c          431 g          353 t
ORIGIN      
        1 agccgggtag ggtcgtcgtg cttggcgccg gaatagtagg aagctcggcg acccgcatcg
       61 cggtcggtat gggagctcag gttactgtga ttaatttgga tattgaacgg ctgcgatacc
      121 tcgatgacca gtaccagggt cgaattgtca cacgcgctgt gagccccgcc accattgaag
      181 aagctgtatg ctcagcagat cttgtcatcg gtgccgtgct cgtccccggc gccaagtcgc
      241 ccaagttggt gtctcgttcg ctggtttcac ggatgaagcc cgggtcggtg atcgtggatg
      301 tttctgtcga tcagggcggt tgtgttgaga cgacaaaacc cacgactcat tcgaagccag
      361 tctacgtcgt tgatggagtc gtgcactatt gcgtcgccaa tatgccagga atagtccctc
      421 gcacatccac ctacgctctc accaatgcga cgttgccgta cctgcttcga cttgcgtctg
      481 acggggtgac ccgagcgatc cagtcagatc cagggctggc caagggaatc aatctcaaag
      541 atgggaagct tacccaccgg agtgtggcgg aagctcatgg gttgcctttt acccctgtct
      601 tgtaagacaa tcacgcttcc gttttctgca ctcggtcttg tcggggcgta gcgcagcccg
      661 gtagcgcact gcgttcggga cgcaggggtc ggaggttcaa atcctctcgc cccgaccaaa
      721 ccgcacttca tgtgggccgc aggctaatta tcgttttatc gtaattgacc ccagcggata
      781 agttcctcaa gaaccttctt tccagtacca aaccaagcat gctcgtattg ccggctctct
      841 atcgcctcat cgtagtcgct cggagaatgg acctcaccgg tttttttcat tccaaaccgc
      901 gtttcgtgtg aattactgcc cattcgggta tgtcgtacat ttcttattcg attcttaata
      961 agtctccgtc acgagcagag tttggcgcag aattgtaaaa cgcgtgacgc accggaagag
     1021 gtgtgagaat atccgcagag caaacccgta agtgactgtt aacactcaag gatcttggta
     1081 agcagatgac gcaatcgact gatgaaccgt cggttcgagc acacatcctc ctgggtggtc
     1141 gtgtgcaggg agtaggattt cgcgcatttg cggcacgaac cgccacacgg cttgacctag
     1201 taggtggcgt ccgcaatctc gacgatggtc gggtcgagct ggacgtggag gggaaaagaa
     1261 tggtgatcga agcacttcta cgtgaattga aaatcggtcc tcctacttcg cgtgtcacaa
     1321 aaattgagat ggagtggagt accacgaccg gacggtattc aaacttcagt atttggtatt
     1381 agaagacgag gaatcatgag tcgaccgcat gacgaggagt ctgagtttag tgaggctcga
     1441 aggcaaaccg tcgacgacat ggatgcctca ggacctgcgg gtgcaccgga tcagggggaa
     1501 cggg
//
LOCUS       DASTWE010000689         1238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1375952, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000689 DASTWE010000000
VERSION     DASTWE010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375952"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFT30_07130"
     CDS             <1..325
                     /locus_tag="VFT30_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU5092440.1"
                     /translation="RSAIIQQHAESLSRICGDHRWYEIATTVDHVMQHEKDLHPNLDF
                     YTAVAYLLMEIPRVLYTPLFVCSRITGWCAHVIEQQDHNRLMRPRALYTGPSRREYVP
                     LDRRT"
     gene            391..>1238
                     /locus_tag="VFT30_07135"
     CDS             391..>1238
                     /locus_tag="VFT30_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU5092441.1"
                     /translation="MSPCIGLPDHITQARKVDGLCPPLPWSSFADFFKSRVYDRALVN
                     RTFLIYCDDDRAVRHTYSYGEFGTVVQSVATFLHDGVGLRPGDRLATILFNHDVNVVL
                     YFAAWILGVTIVPINIEESADKKRYILEHSEASAVCCWHTSLDEAKDLQRELPALRHV
                     IEVNDEGFAEGPKHRAKANAGASPSLGRTSPTPSLDDEALIVYTSGTTGPPKGVVLTV
                     ENLLLDAEAIAAWHRFGADDRLMCVLPIHHVNGTVVTLITPFYCKGSIVLNRKFKSAT
                     FWRRMH"
BASE COUNT          276 a          356 c          321 g          285 t
ORIGIN      
        1 ccgttcagcc atcattcagc agcacgccga atcgctgagc cggatctgcg gcgatcaccg
       61 atggtacgag attgcgacga ctgtcgacca tgttatgcag catgagaaag atcttcatcc
      121 caacctggat ttctacacgg cggtcgccta cctcctgatg gagattcctc gtgttcttta
      181 cacgccgttg ttcgtctgtt cgcgcattac gggatggtgt gcccatgtta ttgaacaaca
      241 ggaccacaac aggttgatga gaccccgcgc cctctatacg ggtccgtcaa ggagagaata
      301 tgtccccctt gatcgccgta cctgaccaca tcacgcagac cctgttgatc aggcctcgcg
      361 tccgcaatat ggggccgtcg aggagagaat atgtctccat gcatcggcct acctgaccac
      421 atcacgcagg cacggaaagt cgacgggctc tgccctccgc tcccatggag ctcattcgcg
      481 gacttcttca agtcccgcgt ctatgatcgc gccctggtca accggacatt tttgatctat
      541 tgcgacgatg atcgggccgt ccgccatacc tacagttatg gagaatttgg gacggtcgtc
      601 caatcagtcg ctactttcct gcacgacggc gtcggattaa gaccaggaga ccgtttggcg
      661 acgatcctct tcaatcatga cgtcaatgtg gtgctgtact ttgcagcatg gatattgggt
      721 gtcacaatcg tcccgatcaa catcgaagag tccgccgata aaaaacgtta catcctggaa
      781 cattccgagg cgtcagccgt ttgttgctgg cacacctctc ttgatgaagc gaaggacctt
      841 caacgagaac ttccagcctt acgccatgtg attgaggtga atgatgaagg ttttgcggaa
      901 gggccgaagc atcgggcgaa ggccaatgcg ggcgcatctc cctccttggg tcgtacatct
      961 cccactccca gcctggatga cgaggcactc atcgtctata cgtctgggac caccggtccg
     1021 cctaaaggtg tggtgctcac cgtagaaaat ctgctcctcg atgccgaggc aatcgccgcc
     1081 tggcatcgat ttggagcaga tgaccgcctg atgtgcgtgc tcccaattca tcacgtgaat
     1141 gggaccgttg tcacactgat cacgccgttt tactgcaaag gcagcatcgt cctgaatcga
     1201 aaattcaaaa gcgctacgtt ctggagaaga atgcacga
//
LOCUS       DASTWE010000690         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1181971, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000690 DASTWE010000000
VERSION     DASTWE010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181971"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1130
                     /locus_tag="VFT30_07140"
     CDS             <1..>1130
                     /locus_tag="VFT30_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5092442.1"
                     /translation="VDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQN
                     YFRMYKKLSGMTGTADTEAAEFAKIYNLDVNVVPTNRQMIRQDYADVVYRTEKEKFAA
                     IVEEIKECHERGQPVLVGTISIEKSERLAGLLNRNGVKHNVLNAKQHEREAEIIAQAG
                     RKGAVTIATNMAGRGTDILLGGNPDFMYKQVLYREENIPEDRKLEIYEGIRSDCDKDK
                     QAVIAVGGLHILGTERHESRRIDNQLRGRAGRQGDPGTSRFYLSLEDDLMRIFASERV
                     SQLMLKLGMEEGVPIEHGMVTRAIANAQKKVEAHNFEIRKQLLEYDDVMNKQREVIYR
                     HRRAVLNGDNLTNDLYDMMDSSVESSLNVYCPPDQYPEEWDV"
BASE COUNT          304 a          304 c          323 g          199 t
ORIGIN      
        1 gtggatgaat tcaccggccg cttaatgccg ggccgacggt ggagcgacgg gctccatcaa
       61 gccgtcgagg ccaaagaagg cgtgaagatc gccaacgaga atcagacgct tgcctcggtg
      121 acctttcaaa actatttccg catgtacaag aagctcagcg gcatgaccgg cacagcggat
      181 acggaagccg ccgaattcgc caagatctat aatctcgacg tcaacgtcgt gccgaccaat
      241 cgtcaaatga ttcggcaaga ttatgccgac gtcgtgtacc ggacggagaa agaaaagttc
      301 gcggcgatcg tcgaggaaat caaagaatgt cacgagcgtg gccaaccggt gttggtcgga
      361 accatttcga tcgaaaaatc ggagaggctc gccgggctgt tgaaccgtaa tggggtcaag
      421 cacaacgtcc tcaatgccaa gcagcacgag cgcgaagccg aaatcatcgc gcaagccgga
      481 cgcaaaggcg cggtcacgat cgccacgaac atggctgggc gaggtaccga catcctgctg
      541 ggcggcaatc ctgacttcat gtacaaacag gtgctgtatc gggaagaaaa catcccggaa
      601 gaccgcaagc ttgaaatcta tgagggtatc cgatccgatt gcgacaagga caagcaggca
      661 gtcatcgccg tgggtggact tcatattctc ggcacggagc ggcacgaaag ccgccgcatc
      721 gacaaccagc ttcgaggccg ggccggccgc caaggcgatc cagggacgtc gagattctat
      781 ctgtcgttgg aagacgacct gatgcggatc tttgcttcgg aacgagtgtc acagctgatg
      841 ctcaagctcg gcatggagga aggcgtcccc atcgaacacg ggatggtgac tcgtgcgatc
      901 gcaaatgcgc aaaagaaagt tgaggcccat aacttcgaaa tccgcaaaca actactcgaa
      961 tacgacgacg tcatgaacaa gcagcgcgaa gtgatttatc gacatcgtcg ggccgtgttg
     1021 aacggagaca acctcaccaa cgacctttat gacatgatgg atagttccgt ggaatcctcc
     1081 ctcaatgtgt attgtccccc cgaccaatac ccggaagaat gggacgtcaa
//
LOCUS       DASTWE010000691         1246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_826671, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000691 DASTWE010000000
VERSION     DASTWE010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826671"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1246
                     /locus_tag="VFT30_07145"
     CDS             <1..>1246
                     /locus_tag="VFT30_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5092443.1"
                     /translation="QAKSVYAPTLRWALGHRTTVLILAVSALIGALALVPSLGTEFIP
                     ILNEGSVAPQTIRLPSVSLPASVEIEKRMQQAIMEFPEVEMVVSKIGRSELGNDPQEV
                     NESDPVVRLRPLDQWTTARTMPELIQKFRERLSGVSGATFLISQPIQQRVDELISGVR
                     TEATVKLFGDDLEMLRNKAQEIAEVLGSVQGVKDIKVEQLFGQPYLTIDIDRGKIARH
                     GINVSDVREIITTAIGGQAATRVYEGQQRFDLFVRFPEQYRDSVETISNILLTDQMGA
                     LIPLADLGTVQLEEGPGRISRDQLQRYVSIGFNTMGRDIGSLVAEAQQKINQRVALPA
                     GYRITWGGSFENMERAMAKLQIIVPITIGLIFFLLYSTFNSLKQATLIILNLPFALIG
                     GVVALWLTKEYLSVPASVGFINL"
BASE COUNT          280 a          325 c          360 g          281 t
ORIGIN      
        1 gacaggccaa gagtgtctat gcaccgacgc ttcgatgggc ccttggtcac cgaacgacgg
       61 tattgatatt ggctgtcagc gcattgatcg gcgctcttgc tttggtgccc tcattgggta
      121 ccgagtttat tcctattctc aacgagggat cagttgctcc tcaaaccatt cggctgccca
      181 gcgtgtcgct tccggcttcc gttgagatcg aaaagcgtat gcagcaggcg atcatggagt
      241 ttccggaagt agagatggtt gtctcgaaga tcggtcgttc cgaattggga aacgatcccc
      301 aggaggtgaa cgagagcgat ccggtcgtgc ggcttcggcc gctggaccag tggacgactg
      361 cgcggaccat gccggagctc atacagaagt tccgtgagcg tttgagcggc gtttccggtg
      421 ccacgttcct tatcagccag cccatccagc aacgggtcga tgagttgatt tccggcgtcc
      481 gcacagaagc gaccgtgaag ctgttcggcg acgatctgga gatgttgcgg aacaaggctc
      541 aggaaatcgc cgaggtgtta gggagcgtgc aaggggtgaa agatatcaaa gttgagcaat
      601 tattcggaca gccatatctc accatcgata tcgatcgcgg taaaatcgcc cgtcatggca
      661 tcaacgtctc agatgtgcgt gagattatca ctactgccat cgggggacag gcggcaacac
      721 gcgtctacga aggacagcaa cgattcgatt tgttcgtgcg gttcccggaa cagtaccggg
      781 acagcgtcga aaccatcagt aatattctac tcaccgatca gatgggcgcg ctgattcctc
      841 tggcagacct cggaaccgtt cagttggaag aaggcccggg tcggatcagc cgagatcagc
      901 ttcaacggta tgtgtcgatc ggcttcaata cgatgggacg agacatcgga agcctggtcg
      961 cagaagccca gcaaaagatc aatcaacgag tcgcgcttcc cgccggctat cggattacct
     1021 ggggcggatc atttgagaac atggaacggg caatggcaaa gctgcagatc atcgttccga
     1081 tcacgatcgg gctgatcttc ttcttgctgt attcgacgtt caactcgctc aagcaggcaa
     1141 ccctgattat cttgaatctg ccctttgcat tgatcggagg cgtcgtggcc ctttggctca
     1201 cgaaagaata tctcagcgtg ccggcgtcag tcggcttcat caatct
//
LOCUS       DASTWE010000692         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_826751, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000692 DASTWE010000000
VERSION     DASTWE010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826751"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFT30_07150"
     CDS             complement(<1..189)
                     /locus_tag="VFT30_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092444.1"
                     /translation="MLHAVTAAGIIDDKRSLMSTGKVIQVIGPVVDVEFPPGHLPNIY
                     NALKVTQAENKTAGTPAVR"
     gene            complement(205..1083)
                     /gene="atpG"
                     /locus_tag="VFT30_07155"
     CDS             complement(205..1083)
                     /gene="atpG"
                     /locus_tag="VFT30_07155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU5092445.1"
                     /translation="MPSLQSLRRKIAAFKNTQKITKAMKMVAAAKLKRSQDRILSARP
                     YAHKMRGVLSNLSRRVNRSSHPLLQKREGKKVEVLVVTSDRGLCGGFNGNIVRKSSEF
                     VRQCESRGLQVDLSIIGRKGRDYFRRRSWPIRQEWTGIFDKLSFEHAIDIGGDLTDNF
                     VKGTFDELYVVYNEFKSAIQQRVIVEKLFPVDAQVEFGAGQMEGMTSGSYLYEPDEAE
                     LLNALVPKHFQVQAYRILLESAAAEHGARMAAMDGATRNAGQLIKKVTLYYNKTRQAA
                     ITKELMDIVGGAEALK"
BASE COUNT          257 a          382 c          350 g          340 t
ORIGIN      
        1 ccgaacggcg ggcgtgcccg cagtcttatt ctccgcttgc gtcaccttca acgcattata
       61 gatattgggc aggtgaccgg gagggaattc cacgtcgacc acggggccga tgacttgaat
      121 gacttttcct gtgctcataa gactcctctt gtcgtcaatg atgcccgcgg cggttaccgc
      181 atgcagcatt cctcagtcca catttcattt cagagcttca gcgccgccga cgatgtccat
      241 gagttctttc gtaatcgcag cctgtctggt cttgttgtag taaagcgtta ccttcttgat
      301 gagctgaccg gcgttgcgcg tcgccccatc catggcggcc atacgggcac cgtgttcggc
      361 ggccgccgac tccagcaaaa tccggtaggc ctgcacttgg aaatgcttcg gcaccagggc
      421 gttcaacagt tccgcctcat ccggctcgta caggtaactg ccgctggtca tcccttccat
      481 ttgccccgcg ccgaactcaa cctgagcatc gacggggaag agtttctcga cgattacgcg
      541 ctgttgaatg gcggatttga attcgttgta cacgacgtac agctcatcga acgtgccctt
      601 cacgaagtta tcggtgaggt cgccgccgat gtcgatcgcg tgctcgaaac tgagtttgtc
      661 gaagatgccg gtccattcct gacggatcgg ccagctgcgc cgtctaaaat agtcgcggcc
      721 tttgcggccg atgatgctga gatccacttg caagccgcgg gattcgcact gccggacaaa
      781 ttcggaactc ttgcggacga tgttaccgtt gaagccgccg cacaacccgc gatcgctcgt
      841 gaccacgagc acctcgacct ttttcccttc gcgcttctgc agaagtggat gtgaagaacg
      901 gttcacacgt cggctcagat tgctcaacac tccgcgcatc ttatgcgcat aagggcgcgc
      961 ggaaaggatg cgatcctgcg agcgtttgag tttcgccgca gccaccatct tcatggcctt
     1021 ggtgatcttc tgggtattct tgaaagccgc aattttacgg cgcaacgatt gaagactagg
     1081 cattgtattc catcagaagc tgtggactga gtgctgagga ctgagattgc aatccggctt
     1141 ctcgccgtac gtctcaatcc tgagtcttcg atactcagtc ctgtgcttac gctttcgctc
     1201 cgtatcccat cttctgtttg aaagtcgtaa tgatctcttt caagcggcca ccgaccttat
     1261 cgtcgatctt accgatggtg gtgacttctt ttttcagttc cggatgattc tgctggatat
     1321 agtggagca
//
LOCUS       DASTWE010000693         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1117703, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000693 DASTWE010000000
VERSION     DASTWE010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117703"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFT30_07160"
     CDS             <1..260
                     /locus_tag="VFT30_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092446.1"
                     /translation="SRPRRLRDSTGREYSYLLHWGAPELSRDDHYRVSGSNKLDFGLE
                     HFIEDLGTLLGAYLADLNKLPELQAKALATWPKIEVQDFRM"
     gene            complement(357..638)
                     /locus_tag="VFT30_07165"
     CDS             complement(357..638)
                     /locus_tag="VFT30_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092447.1"
                     /translation="MEEPVKGLRFERIANGRHYNVVFHIGSTYVPVSDDTVEELREQS
                     LLPAERFLDLLIDRVGYSSYLKDQIRNELKATGDPTTQITVLQGAIREL"
     gene            766..2220
                     /locus_tag="VFT30_07170"
     CDS             766..2220
                     /locus_tag="VFT30_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEU5092448.1"
                     /translation="MTATELIEWWITRLEAERARLIETGQDAPVVASQGRLVQSTGGL
                     HLYEFIVPADVQLSLDLPVSVVPDDESDTTEGIVLRQAGNSISIQLVDALGRDVPSVT
                     LVPDQAGFVSTSISRLRDMLAKPDLYHLGLTERLASLLQTQEVGAGAFPYASSVFTTV
                     YSDDRLVRRQMLGNLTMELIRANKRILLISPDHQECDEIVGIVGRTMKAGGLNHKMWI
                     TRYELPIASLAGGVDLHELGFEAQMHQFYAKSQGDKASLKHKYDRFRELAPFLSQKEA
                     KQKDLDEVRLLEWRLVTQLRDLQVRMADVRKTLEEFENLPLFQRLTMQAVGKNAESLK
                     QYCALYQSQMDQLNEELDVAQSRIQQLAPEAAVPKGTRAEFEDLKEHMTKLGGTKKVR
                     ELLAAEENPNRQAFIQNRRFVAATPARVASDPLFSRVRFDVLMVDEAPRIAAPSLLAA
                     AGLVRERIIVSGDPRDIAAAGQWAISQGASPTVH"
     gene            2414..2500
                     /locus_tag="VFT30_07175"
     tRNA            2414..2500
                     /locus_tag="VFT30_07175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2448..2450,aa:Leu,seq:cag)
BASE COUNT          609 a          691 c          720 g          609 t
ORIGIN      
        1 ccagtcggcc tcgacgtctt cgtgattcga ccggtcgtga gtactcatac ttgttgcact
       61 ggggagctcc ggaactatcg cgcgatgacc actacagagt ttctggaagc aataagctcg
      121 attttggctt agaacatttc atagaagatc ttggcactct attgggcgcc taccttgctg
      181 accttaacaa gttgccagag ttacaggcaa aagccttggc aacatggcca aagatcgaag
      241 ttcaagattt cagaatgtga agagctgttt gttctgcagg atgccgtgga gttctcttcg
      301 cgtaggttcg aaggagaaca aggaggagaa gagagcgagc gaaaatatag caggcgttac
      361 agttctctga ttgcgccctg tagcacggta atctgcgtgg tggggtcgcc cgtcgctttc
      421 agttcgttgc ggatctgatc tttcaggtaa gatgaatagc ccacgcggtc gatcaggaga
      481 tccaaaaatc gttcggccgg cagcaggctt tgctccctga gttcttctac cgtgtcgtca
      541 ctcaccggca catatgtact gccgatgtgg aagacaacat tgtagtgacg tccattagcg
      601 atgcgctcaa accgtagacc cttcacaggt tcctccacag aagccgctca ttctagacaa
      661 gatctggtcg tgaagacaag ggcaacctat ctcggtcctg tggtggacag gtatcgccgc
      721 gggcatttat catgacctta tcttgggatg caggatcaat ccttcatgac ggcaacagaa
      781 ctcattgaat ggtggatcac ccgcctcgaa gccgagcgag cccgactgat tgaaacaggg
      841 caggacgccc ctgtggtggc gtcgcaaggt cgcctggtgc aaagcaccgg ggggctgcat
      901 ctgtacgagt ttatcgtgcc tgccgacgtc caactgtccc tcgacctgcc ggtatccgtc
      961 gtcccggacg atgagtcgga cacgacggaa ggaattgtct taaggcaggc cgggaacagc
     1021 atatctatcc aactggtcga tgcccttgga cgggatgttc cttctgttac ccttgttccg
     1081 gatcaggccg gctttgtcag cacgtcgatc tcccgtttga gagacatgct ggcaaagccg
     1141 gacctttatc atctcgggct gaccgagcgt ttggcttcac tgttgcagac gcaggaagtt
     1201 ggggccgggg cattccccta tgcttcatca gttttcacaa cagtgtactc ggatgaccgg
     1261 ttagtccgtc gtcaaatgct cgggaatctg acgatggagc tcattcgcgc caacaaacgc
     1321 atcctcctga tcagtcccga tcatcaagaa tgcgatgaga ttgtggggat cgtggggcgt
     1381 accatgaagg ccggcggatt gaaccacaaa atgtggatca ctcggtatga actgccgatt
     1441 gcctcgctag ccggcggcgt cgatcttcat gaactggggt tcgaagcgca aatgcatcaa
     1501 ttttatgcca agtcacaagg tgacaaagcc tccttgaaac ataagtacga ccgcttccgt
     1561 gagctcgcac cttttttgtc tcagaaagag gcgaaacaga aagatctaga cgaagtccgc
     1621 ctattggagt ggcgcctcgt cacccaattg cgagatcttc aagtgaggat ggccgatgtc
     1681 cgaaagacgc tcgaggaatt cgagaactta cccttgtttc aacgtttgac gatgcaggca
     1741 gtcgggaaga atgccgaatc cctcaaacag tactgcgcgc tgtatcaaag tcagatggac
     1801 cagctgaacg aagaacttga tgtcgcccaa agccgcattc agcagcttgc tcccgaggcg
     1861 gccgttccaa aggggactcg ggccgagttt gaggacctca aagagcatat gacgaaattg
     1921 ggcggaacca aaaaagtccg tgaattgctg gcagccgaag aaaatccgaa ccgccaggcc
     1981 ttcattcaga atcggcgatt cgttgctgcc acccccgcgc gcgtcgcgag cgatcccttg
     2041 ttcagccggg tgcgcttcga tgtcttaatg gtcgatgagg ccccgaggat cgcggcgcct
     2101 tcacttctgg ctgcggcagg actcgtacgt gaacgtatta tcgtcagcgg cgatccgcgt
     2161 gacatcgctg ccgcgggaca atgggctata tctcagggtg cctcccctac tgtacattga
     2221 gcagcagccc tgacacttgc ttgacgtgga taacgagaat tcaggataat ccgattcctt
     2281 tagactccga cttcttggca cgttcaaatg gtcttgaacg agccataggg tgccctgcga
     2341 acatgacgaa gatgttttgt acgttgcctt gaagcgggca cggagactga aggtcatttt
     2401 cagagtacct attgccgaag tggcggaact ggcagacgca ctagattcag ggtctagcgc
     2461 cctcacgggt gtccgggttc aaatcccggc ttcggcacca accatcctgt agttggctca
     2521 gagccaattc acagacagcc tccttaggaa ctgtagacga tctacagtct aaggaggttt
     2581 tcatgtctac cgtttttctt tcccgaaatc gcaaatcgct ctatcatcg
//
LOCUS       DASTWE010000694         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_988562, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000694 DASTWE010000000
VERSION     DASTWE010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988562"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFT30_07180"
     CDS             <1..395
                     /locus_tag="VFT30_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEU5092449.1"
                     /translation="ELQIAIGGQFVANLRGDGLIIGTPTGSTAYSLSAGGPIMTPALQ
                     ALILTPICPHTLTHRPLIVPGDVVIEVTLTSKDDGSMATLDGQVGVAMTQGDTTVIQA
                     SDYRTRLIRFPESHYYEVLREKLKWGHG"
     gene            complement(416..1765)
                     /locus_tag="VFT30_07185"
     CDS             complement(416..1765)
                     /locus_tag="VFT30_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5092450.1"
                     /translation="MSGTQEHVIIVAGAGPAGMAVASALSKAGHEIIILNRDIKFGGL
                     AEYGIFPSKLKLRGGLKKQYWELLQQKNVHYFGNVSIGNGKDLTVEDVRRLGASAVVF
                     TIGAQGTKAIGVEGDSAQGVFHAKDVVYHFNRLPGFGDRPFEMGKHVAVIGAGDVMVD
                     IAHWLIRYKKVERVTAIVRRGPVERKYNPKEIRSICANMDLEGIKAEFERIKDRLAKV
                     GQNPDEVLQAFTDEFTKCEPKVTETKMGFRFLASPKRILADSNNRVRGLEMEDNRLDP
                     KGEDTIAVGLKQYYEFPCDSVIFAVGDKVDETVGLPYKNGMFTTNPNKTGNDPDDALF
                     QAYDESTGKVMDGVFLAGWARKASEGLVGVAKRDGDWCAEVVERYLMTKSGTDAKIVL
                     DHLHSLLGKRQSRPVDMNGLRALDVAERSFAGKADCIGEFKFSANHDMLKYIEQGNA"
     gene            1956..>2546
                     /locus_tag="VFT30_07190"
     CDS             1956..>2546
                     /locus_tag="VFT30_07190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5092451.1"
                     /translation="MSTDESSSLQLVEKALAPPIRYCRIWKSDCVLPLKTLCTDEAYI
                     VSDFELAEELIARGADSARIAISPHHSTEDQYLGYSQVAAGKQLIPASPFDFKQSIVI
                     VLPTYNERANLEALVHAISQYLVTDILIVDDSSPDGTGELADQLSCKHGHVHVLHRSK
                     KEGLGPAYLAGFKWALQRNYSLIIEMDCDFSHAPWDL"
BASE COUNT          615 a          710 c          602 g          619 t
ORIGIN      
        1 tcgaactaca gatcgccata ggaggccagt tcgtggcaaa tctacgaggt gacggcctaa
       61 tcatcggaac accgacaggc tctaccgcct attccctttc tgctgggggg cctattatga
      121 cccccgcgct tcaggcgctc atcctaaccc ccatctgccc acatacgttg acgcatcgcc
      181 cgttaatcgt accaggcgat gtggtcattg aagtgacgtt gacgagtaag gatgacggat
      241 caatggccac gctcgatggg caagtcggcg ttgccatgac acagggtgat acgacagtaa
      301 ttcaggcttc agactatcgg acgagattga ttagatttcc agaaagtcac tactatgaag
      361 tattgcggga aaaattgaaa tgggggcacg gatgaagaga aaatattcaa agaccttatg
      421 cgttcccctg ttcaatatat ttgagcatgt catgatttgc tgaaaacttg aactctccga
      481 tacagtcggc tttcccggca aatgagcgtt ctgccacgtc gagtgctcgt aagccattca
      541 tgtcgactgg acgactttgc cgctttccga gaagcgagtg caagtgatcc agcacgattt
      601 tggcatctgt gccgctcttg gtcatcagat agcgttcgac gacttccgca caccaatctc
      661 cgtcccgctt cgcgacgccg acgaggccct cactagcttt tcgagcccaa ccggccaaaa
      721 atacgccgtc catcaccttg cctgtagatt catcgtaggc ctgaaagagc gcatcatcgg
      781 gatcattgcc ggtcttattc gggttcgtgg taaacatccc gttcttatat ggtagaccga
      841 cggtctcatc caccttgtcc ccgacggcaa aaataaccga atcacaggga aattcatagt
      901 actgcttcaa gcccacggcg atggtatctt cacccttagg gtcaagccgg ttatcttcca
      961 tttcaagccc gcgaacccga ttattgctgt cagctagaat acgcttcgga gatgcaagga
     1021 aacggaaccc catctttgtc tcggtcacct tcggttcgca tttggtgaac tcgtcggtga
     1081 atgcctgtaa gacttcatcg ggattctgtc cgaccttggc aagacggtcc ttgatgcgct
     1141 caaactcggc tttgatcccc tcgaggtcca tattggcaca gatgctgcga atttcttttg
     1201 gattgtactt gcgctccacg gggccccgtc tgacaatcgc cgtgaccctc tcaactttct
     1261 tgtagcggat caaccagtgg gcaatatcca ccatcacatc ccccgccccg atcacggcga
     1321 catgttttcc catttcaaat gggcgatcgc caaagcccgg taaacgattg aagtgataga
     1381 ctacatcttt tgcatgaaac acgccttgag ccgagtcacc ttcgacgcca atggccttgg
     1441 ttccctgcgc accgatcgtg aaaacaaccg cgcttgcgcc caacctgcgc acatcttcga
     1501 ccgtgaggtc ctttccgttc ccgatggaga cattcccaaa ataatggacg ttcttctgct
     1561 gaagaagttc ccagtactgt ttcttgaggc caccacggag tttcagtttg gaagggaata
     1621 ttccatattc agccaatcca ccgaatttga tgtcacgatt gagaataatg atttcgtggc
     1681 cggctttcga aagcgcgctg gcaacagcca taccagccgg tcccgctcct gccacgataa
     1741 tcacatgctc ctgtgtcccg ctcatacagt gttccttaag aaattaggta aagaaaaacc
     1801 tggaatatca aaggatcgcg gactgtagca tagccgttcc cgaactgtca aaaagcacgg
     1861 cggcgggaca cgacaatcgg atggatttcg acttgcccgg gtatccactg tcgtttacaa
     1921 tgccgccgcc ggatcaagtt acatgagaat tctctatgtc cactgacgaa tcatcctctc
     1981 tgcaattagt agagaaggct ctggcacctc ccatccgcta ctgtcgcatc tggaagagcg
     2041 actgcgtcct tcctctcaag acactgtgca cggatgaagc atatatcgtc tcggatttcg
     2101 agcttgctga ggaactgatc gcccgaggtg ccgacagcgc acgcattgct atcagcccac
     2161 atcattctac tgaagaccaa tatttgggat attcccaagt agcggcaggt aaacaactga
     2221 tccccgcctc accgttcgat ttcaaacaat cgattgtcat tgtcctgcca acttacaatg
     2281 aacgcgccaa tcttgaggca cttgtccatg cgatcagcca atatcttgtg actgacatct
     2341 tgatcgttga tgatagctcg ccggacggaa ccggagagtt agccgatcaa ctcagttgca
     2401 agcacggaca tgtacatgtc ttgcaccgat cgaaaaaaga agggcttgga ccggcgtatc
     2461 tggctggctt caagtgggca ctgcagcgaa attacagcct catcatcgaa atggattgtg
     2521 acttcagcca tgctccctgg gatctg
//
LOCUS       DASTWE010000695         1495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_794320, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000695 DASTWE010000000
VERSION     DASTWE010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794320"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..350
                     /locus_tag="VFT30_07195"
     CDS             123..350
                     /locus_tag="VFT30_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5092452.1"
                     /translation="MNADTFKGKWSQFKGELKRQWGKFTDDDLMQIEGNYDKFKGKVQ
                     ERYGDQKDELETWANKWYGEAKDTAKQAPNR"
     gene            408..1031
                     /locus_tag="VFT30_07200"
     CDS             408..1031
                     /locus_tag="VFT30_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5092453.1"
                     /translation="MFTLTPVKTFVGAVVLTFLTVFFQIGEAQATHEAGSDHTNWAGM
                     SQVGIGFFSIGGRATYFDPKEGEDKWFGGAQLRIHPSPYLAIEGSADYRREEFDGGTK
                     SHTYPVQGSLLIYPLGTTRVAPFILGGGGWYFSTVDGPGNFSDTQHRFGGHVGGGLQF
                     FISDHFSIDSTYRHIWLERLDSRGQNLEDKKFNDNGHMVTIGLNIHF"
     gene            1070..>1495
                     /locus_tag="VFT30_07205"
     CDS             1070..>1495
                     /locus_tag="VFT30_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092454.1"
                     /translation="MIRTRSSVGAGFLKTRPSFLVLKQFLFCLRTPSLILCLSLLGAP
                     STWAQVTTDEYIAGFATAVLQHEFKAADSTLQVHDGVIRISARSLGGLDREKVKRSLT
                     DIAGVKEVVIEEDEELAASPTPQRGVLVDVPETESSFLPR"
BASE COUNT          379 a          405 c          377 g          334 t
ORIGIN      
        1 ctatgcaccg ccccctccgc tgcttactag ggaacaggct agtcgatgct cgccatatta
       61 tgcgttaaga aatacatggg aatgctgggg agaacatatt aatcacaaat gaggaggctg
      121 atatgaacgc ggacacattt aaaggcaaat ggagtcaatt caaaggggag ctcaaacgac
      181 aatggggcaa gtttaccgat gacgatctga tgcaaatcga aggaaactac gacaagttca
      241 aaggtaaagt tcaagaacgc tatggcgacc agaaggatga gctggaaacg tgggctaaca
      301 aatggtacgg cgaagccaag gacaccgcaa aacaggcgcc aaacaggtag agggaaccag
      361 ctccaccaga agataacgac tagccgacat caaggaggca cgttttcatg tttacattga
      421 cgccggtcaa gacattcgtc ggcgcagttg ttctcacttt cctcaccgtc ttcttccaga
      481 tcggtgaagc gcaggccaca cacgaggctg gctcggacca taccaactgg gcaggtatgt
      541 cgcaagtcgg catcggtttt ttctccattg gtggtcgtgc cacctatttc gatcctaaag
      601 agggagaaga caaatggttc ggcggcgcgc aactgcgcat tcatccgtcg ccctacctcg
      661 ccatcgaggg ctccgccgac tatcgtcgag aagaattcga cggaggaacg aagtctcata
      721 cctaccccgt ccaaggatcc ttactcatct atcctttggg cacgactcgt gtggctccgt
      781 tcattctcgg cggcggcggt tggtattttt ccacggtcga tggccccggt aatttctctg
      841 acacgcagca tcggttcgga ggccatgtcg gtggcggatt gcaattcttc atttccgatc
      901 atttctcgat tgacagcacc tatcgccata tctggctgga acgtctggac tccagaggtc
      961 agaaccttga ggataagaaa ttcaatgaca atggccatat ggtcaccatc ggcttaaaca
     1021 ttcacttcta gcggctatgc cgtcctccgc agaaactcga cgccgatgga tgattcgcac
     1081 gagatcatcc gtcggcgcag ggtttctaaa aacacgacct tcatttcttg tcctcaaaca
     1141 attcttattc tgtctgagaa ctccctcgtt gattctgtgc ctctccctgc tcggcgcacc
     1201 ttcaacatgg gcgcaagtca ccaccgatga atatattgca ggttttgcta cagccgtcct
     1261 acagcatgag ttcaaagcag ccgattcgac gttgcaagta cacgatggcg tgatcagaat
     1321 cagtgcccga tccctcgggg gactcgaccg tgaaaaggta aaacgctcgc tgaccgacat
     1381 tgccggtgtc aaggaagtcg tcatcgaaga agacgaagaa ctcgcagctt cccctacacc
     1441 ccagcgaggc gtcctggtcg atgtcccaga gactgaatcc agcttcttgc ctcgc
//
LOCUS       DASTWE010000696         1710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1537744, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000696 DASTWE010000000
VERSION     DASTWE010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537744"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFT30_07210"
     CDS             complement(<1..261)
                     /locus_tag="VFT30_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEU5092455.1"
                     /translation="MAIAIEPVERNQLIKHPDVVISVGVVAIIMVMLLPLPRFLLDLL
                     LSFDITLSVVILLVGLQVRRPIEFSVFPSVLLMITLFRLSLNI"
     gene            complement(284..1360)
                     /gene="flhB"
                     /locus_tag="VFT30_07215"
     CDS             complement(284..1360)
                     /gene="flhB"
                     /locus_tag="VFT30_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEU5092456.1"
                     /translation="MAEDRSNKTEPATPKRKEEARKKGQIAVSRDLSTAVILLSGIGL
                     LAAMLPVGIQKMIEMTRQGLTLSFPVDFHEGMTIEQVYSIVIHTGITVVTLSLPVVMG
                     ILVMGSAASLLQTGLLWRADAVQPDGARISPIKGLSRLFSLRSVMELVKGLLKIAIVT
                     GIGLWVARYDVLQIPGLIEFDIGTVLQVTGRLSLKVGLTVAGAIAVLAGLDYFYQRYE
                     WERSLRMSKDEIKEEHKATEGDPLIKSRVRTIQREMTKKRMMAAVKTADVVITNPTHL
                     AVALKYDTSAMGAPVVVAKGAGLVAERIRELARHHGVPVVEHKFVARTLFKLVDIGKE
                     IPNELYRAVAEILAFVYRARGMTP"
     gene            complement(1353..>1710)
                     /locus_tag="VFT30_07220"
     CDS             complement(1353..>1710)
                     /locus_tag="VFT30_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEU5092457.1"
                     /translation="LLLVATILSSYEAIPAFGASLPGGMGDDILRLFQHMFMVGLKLA
                     APVLVVVLLINILLALLGRAVSQINVFVLSFPVTIAGGLAVLGLSIPFVVELLVREIE
                     RLQFTIHGLMKALGHG"
BASE COUNT          420 a          501 c          437 g          352 t
ORIGIN      
        1 aatgttgagg gacagcctaa ataaggtgat catcaataga actgatggaa acaccgagaa
       61 ctcgatcgga cgccgtactt gaagtccgac gagcaaaatc acgaccgaca aggtgatatc
      121 gaagctcaac aataagtcga gcagaaagcg aggcaatggc agcagcatca ccatgataat
      181 ggccaccacc ccgacggata tgacgacgtc cggatgcttg atcaactggt ttcgttctac
      241 cggttctatt gcgattgcca tagttgttga gcctacgaaa tgatcacgga gtcatccctc
      301 tggcgcgata gacaaacgcc aggatctccg caaccgcacg gtagagctcg ttgggaattt
      361 ctttgccgat atcgacgagt ttgaacagcg ttctggccac gaatttatgt tcgacgaccg
      421 gcactccgtg atgtcgagcc agctcacgaa tacgttcagc gaccaagccg gctcctttgg
      481 cgaccacaac aggtgctccc atcgcagacg tatcatattt cagagcaaca gccaggtggg
      541 tcggattggt aatcactacg tccgccgttt tcacggcggc catcatgcgc ttcttcgtca
      601 tctctcgctg gatggtccgc acgcgactct tgataagcgg gtcaccttcc gtagccttgt
      661 gttcctcctt gatctcgtcc ttcgacatcc gcaggcttcg ttcccactca tagcgttggt
      721 agaagtagtc gaggccggcc agaactgcga tcgccccggc gacagtcaat ccgaccttca
      781 gcgagagccg tccggttact tgtagaacgg taccgatatc gaattcgatc aacccaggaa
      841 tctggagcac gtcataccgt gccacccaaa ggccaatacc tgtcacgatc gcgatcttca
      901 gcagcccctt cacgagctcc atcacagaac gcagtgagaa caatcgggaa agccctttga
      961 tcggactgat gcgcgcgccg tccggttgaa cggcatcggc acgccacaat aacccggttt
     1021 gcagcagcga cgcagcgctc cccatcacca gaatgcccat cacgaccgga agactcagcg
     1081 tcaccaccgt gatgccggtg tgaatgacga tcgagtacac ctgctcgatc gtcattcctt
     1141 catggaagtc tacagggaac gagagtgtaa gcccttggcg ggtcatttcg atcattttct
     1201 gaatgccgac cggcagcatg gccgccagca gtccgatgcc gctcaagagg atgactgcgg
     1261 tagacagatc tcgactgacg gcgatctgcc ctttcttgcg cgcctcctct ttgcgtttcg
     1321 gcgtcgcggg ttctgtctta ttactccggt cctcagccat gtcccaacgc tttcatgagt
     1381 ccatggatcg tgaattgcag tcgctcgatt tcccgaacaa gaagctccac tacaaacggt
     1441 atggacaatc ccaacaccgc caagccaccg gcgatggtga cgggaaagct cagaacaaac
     1501 acattgatct gactgactgc acgtccaagc agcgcgagaa ggatattgat caggagaacg
     1561 acgacgagca cgggtgccgc caatttcaat cccaccatga acatgtgctg aaagaggcga
     1621 aggatatcat ctcccattcc tcccgggagc gaagcgccga acgccggaat agcctcatag
     1681 ctggaaagaa tcgtggccac cagaagcaga
//
LOCUS       DASTWE010000697         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1117868, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000697 DASTWE010000000
VERSION     DASTWE010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117868"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFT30_07225"
     CDS             complement(<1..485)
                     /locus_tag="VFT30_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5092458.1"
                     /translation="MNERDRSLWYKNAVMYGLDVATFQDSNGDGIGDFPGLISRLDYL
                     ADLGVTCLWLLPFFPSPNRDNGYDVTDYFGVDPRLGTFDEFRLFVHQAGERGLRVLID
                     LVMDHTSDQHPWFRAARQDPASRYRRYYTWTDNPPPLEPGKGNIFPDEEPSVWTFDEV
                     AG"
BASE COUNT          258 a          280 c          279 g          240 t
ORIGIN      
        1 ccggcgacct cgtcgaaggt ccatacggag ggttcctcgt cgggaaaaat gttgcctttc
       61 cccggttcaa gcggcggcgg attatccgtc catgtgtaat agcgtcgata ccgggacgcc
      121 gggtcctgcc gggcggcccg gaaccaagga tgttggtcgg atgtgtgatc catgacgagg
      181 tcgatcaaca cgcgaaggcc acgttccccc gcttggtgca cgaatagccg gaattcatcg
      241 aacgtcccga gtcgcggatc gacgccgaaa tagtcggtga cgtcatatcc gttgtcgcga
      301 ttgggtgagg gaaaaaaagg caacagccac aggcacgtca cgccgagatc ggccaaataa
      361 tccaatcggc tgatcaagcc tgggaaatca ccgattccgt ctccgttgga gtcttgaaat
      421 gtcgccacat cgagaccgta catgacggca ttcttgtacc agaggcttcg atcacgttcg
      481 ttcatgacat tgaaggcttg tttctatttc taaagtggat tagacgtcga acctacccct
      541 tggaatgttg cgcaaccacg acaagtgcca ttagccattc cgacgaaggt ctttcgccga
      601 ccgtaaaaaa gctccgcttc ctttcgaaag cggagctttc cgaacttaca attttacgtt
      661 gtgtcagtta acgtttccgt tatgtgttca caacgtttcc actgcaagct cttacttcca
      721 ccatactctc cacagtctat ggatcataca ggtaaaccac ctaggagtag tatggacaaa
      781 cccggaacca atcgggccta ggcatgcaca ccgttcggca gttgagacac taggggttac
      841 tctagtacta ttccctcttc taagtgccta taagccgaat gccccggatt gggaagcaat
      901 cggtgcgttc cgcctcaatc attcatgcgt cgcgactcga caccgaggag aagccaaatg
      961 ccgttgaaaa gcaagatgtt gctgaagaac ggatcgcgtg aagtagagct ggatccgaga
     1021 gtgctgctgc aggtcttgaa gtcaatcagg agaggag
//
LOCUS       DASTWE010000698         1873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_342105, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000698 DASTWE010000000
VERSION     DASTWE010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342105"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFT30_07230"
     CDS             complement(<1..167)
                     /locus_tag="VFT30_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU5092459.1"
                     /translation="MGKILVKRVYEPATKSDGFRVLVDRLWPRGLSKENAHIDLWLRE
                     IGPSTTLRKWF"
     gene            complement(173..1012)
                     /locus_tag="VFT30_07235"
     CDS             complement(173..1012)
                     /locus_tag="VFT30_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5092460.1"
                     /translation="MPLTTYNNVPIPSFMYGTAWKKEATTGLVLQAVEAGFIAIDTAN
                     QLVHYDEARVGEALVQLAKQGISRDKLFLQTKFTPINGQDHRLPYDARANITTQVQQS
                     FESSLVHLHTDYLDSYVLHGPYSRRGLGTEDWEVWAAIESLYDAGKTKMIGISNITAD
                     QLTLLCVRAKHKPMVVQNRCYAAFGWDKDVREICRTQHIIYQGFSLLTANREIFIDPE
                     IRAIAARYEATLAQLIFRFAMQIGMLPLTGTTNPQHMKEDLQSDRFTLLSEEIQQIET
                     IGL"
     gene            1369..>1873
                     /locus_tag="VFT30_07240"
     CDS             1369..>1873
                     /locus_tag="VFT30_07240"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5092461.1"
                     /translation="MTFRSGTGADAPACGRIGYEAFKAIAEQHNFPPDFPSAEIAIGL
                     YAMMLGRSDIFSVVGECDGQIVCSNFLWEGDSIAGVGPITVLPAAQNTSLGRRLMQSV
                     LDRAESRNQVGVRLVQAAYHNRSLSLYTKLGFQAREPLSILQGLPLNMEPSGYRVRQA
                     RLDDLEAC"
BASE COUNT          392 a          519 c          509 g          453 t
ORIGIN      
        1 ttgaaccatt tccgtagtgt cgttgagggg ccgatttctc ggagccaaag atcgatatgc
       61 gcgttctctt tcgacaagcc ccgcggccag agccgatcaa caaggacacg gaagccatcg
      121 gacttagtcg ctggttcata gacacgtttg accaggatct tacccatgag tcctagaggc
      181 cgatggtttc gatctgttga atctcctccg agagcaaggt gaatcgatcc gattgcaggt
      241 cttctttcat atgctgagga ttcgtcgtgc cggttagagg cagcatgccg atttgcatcg
      301 caaaccggaa aatgagctgg gcaagggtag cctcataccg agccgctatg gctctgatct
      361 caggatcgat gaagatttct cgatttgcgg tcaggaggga gaagccttga taaatgatgt
      421 gctgcgtccg gcaaatctcc cgcacgtctt tatcccaccc gaaggccgca tagcagcggt
      481 tctgcaccac catcggcttg tgtttggccc tgacgcagag caaggtcagc tgatcggccg
      541 tgatgttgct gatcccgatc atcttcgtct tgccggcatc gtagagggat tcaatcgcgg
      601 cccagacctc ccaatcctct gtccctaagc cgcgccgtga ataggggcca tgcagcacat
      661 acgagtcgag atagtcggta tgaagatgta ccaaggagct ttcgaaagat tgctgcacct
      721 gcgtggtgat gttcgcccgc gcatcgtacg gcagacgatg atcctgaccg ttgatgggcg
      781 tgaacttcgt ttgcagaaat agcttatcgc ggctgatgcc ttgcttggct agttgtacga
      841 gcgcctcccc cacccgcgct tcatcgtagt gaacgagttg atttgccgta tcgatcgcga
      901 tgaacccggc ttccaccgct tgcagcacca gtccggtcgt ggcctctttc ttccaggccg
      961 tgccgtacat gaaggaggga atcgggacgt tattataggt agtcaatggc atcgcagatc
     1021 tcctgtcaga tcttgtacca tacacagttc atgccggtgg tctcaacgtg agaattactg
     1081 aagggtggct aggaagtagg aaggcaatac cacgatcacc aaactctgca tcagtgaccg
     1141 ctgaagtagg aacgccgcat cagagcactc tttcttctgt ccagcactcg tcgatgtgta
     1201 tctgtttttg tcgcctcaag ctcgatcatg attgcttgtc cattgacccc tctttgcagc
     1261 tgtggtaaca gaggcatcca tgctggccgt acaaccacat cgtgagtcgc gtgatgtcgc
     1321 ttctcacctg ctacgaccct accccttttc cttgtccgac ccatgcacgt gacattcaga
     1381 tctggcactg gtgctgacgc cccggcctgc ggaaggatcg gctacgaagc gttcaaggcg
     1441 attgccgagc aacataactt tcctccggac ttcccttctg ctgagatcgc gatcggactg
     1501 tacgcaatga tgctgggacg gtctgacatc ttttccgtcg tgggagaatg cgatggccaa
     1561 atcgtgtgca gcaattttct gtgggaaggt gattcgattg cgggagtcgg tccaatcacg
     1621 gtccttccag cagcacaaaa tacctcgctt ggacgcaggt tgatgcagtc ggtcttggac
     1681 cgcgccgaaa gtcggaatca ggtgggtgtt cggcttgttc aagccgctta tcataaccgt
     1741 tcattgtccc tgtacaccaa gctcggattc caagcgcgcg agccgctttc cattctgcag
     1801 ggcctgccgc tcaacatgga gccatccggc tatcgagtcc gccaagcgcg cctggacgac
     1861 ctagaggcat gca
//
LOCUS       DASTWE010000699         1472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1602678, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000699 DASTWE010000000
VERSION     DASTWE010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602678"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VFT30_07245"
     CDS             complement(<1..867)
                     /locus_tag="VFT30_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5092462.1"
                     /translation="MTAMTHRDRLVLAIAAACFLIILIIEEFAPVNVVGAYGYVLPIL
                     LVTTLRNRTIMLVTVLACVVATYSGLLQPTKPGRFQAAVINRSVVVGVLLLVAYLGVS
                     WEERKAREETARAALARETENLLKANTQLVQAKDQLNRSERLAAVGQLVASVAHEVGT
                     PLHSIAWHVQALAEEPGATPEMRKRVAIIDDQLTRVVRIIQDLLSSTRPRQPEPQWLP
                     VEEVVNPSATLMEPAFHEKGIALTVDIPKDLPMVWADREKIHQVLVNVLANALAATPA
                     QGKVTVVAGSREA"
     gene            complement(946..>1472)
                     /locus_tag="VFT30_07250"
     CDS             complement(946..>1472)
                     /locus_tag="VFT30_07250"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU5092463.1"
                     /translation="RVFYFEREPGGGWKPPAHYPAQSLAVATTHDLPTLGGYWEGVDI
                     ETRSALGLFPSEDARRAMWAERHREKAGILAALKSQGLLPPGISEDPVQTPIMTTELM
                     EGVHQYLARTSAWIVLVNIEDVIGIRTQTNLPGTVDQHANWCRKLSLTVEDLIQDSRL
                     ERLATLLRLARPLV"
BASE COUNT          318 a          448 c          385 g          321 t
ORIGIN      
        1 cgcctcgcga cttcctgcca cgacggtaac ttttccctgg gcgggtgttg cggcgagggc
       61 attggctagg acattcacca atacctgatg gattttttcc ctgtccgccc aaaccatagg
      121 aaggtccttt ggaatgtcga ccgttagagc gatccctttc tcatggaagg ccggttccat
      181 cagggtcgca gatgggttga ccacttcttc caccggaagc cattgcggct ctggctggcg
      241 cggcctggta gaagacagta aatcctgaat gattcgaacc actcgcgtga gctggtcatc
      301 gatgatggcc actcgcttcc tcatttccgg tgtcgcacca ggctcctcgg caagagcctg
      361 gacatgccac gcaatcgagt gaagtggcgt gcccacttcg tgcgccacgg acgccacgag
      421 ctgtcccaca gccgccaacc gttccgaccg gttcagttga tctttcgcct gcaccagctg
      481 cgtgttggct ttgagcaagt tctccgtttc tcgtgccagc gctgccctcg ccgtctcctc
      541 tcgcgccttc cgctcttccc aactcacacc cagataagcc accaacagta agacgccgac
      601 gacgacgctc cgattgatga ccgcggcttg aaatcggccc ggcttcgtcg gttggagtag
      661 acctgaatag gtcgccacga cacaggccag caccgtcacc aacatgatgg ttctattccg
      721 aagggtggtc accaacaaga tgggtaatac gtatccatac gcgccgacca cgttgaccgg
      781 ggcaaattct tcaatgatta ggatgatgag aaaacaggca gccgcgatgg caagcacgag
      841 gcggtcacga tgcgtcatgg cagtcatcgg ctcgctccgc caagtcgagg atcacagcct
      901 ctgattgaga atatcgataa cgtacggatt ccttcacaga agtacttaca caagaggacg
      961 ggctaaacgc aacagagtcg cgagtcgttc aagtcgtgag tcttggatca agtcctccac
     1021 ggtcagactc aacttccggc accaattcgc atgctggtcc accgttcccg gcaggttggt
     1081 ttgggtgcga attccgatga catcttcgat attaaccaag acaatccatg cgctggtacg
     1141 ggccagatac tgatgaaccc cctccatcaa ctcagtcgtc ataatcggcg tctgtaccgg
     1201 atcctccgat atacccggcg gcaagagtcc ttgggacttc aatgcagcaa ggatccctgc
     1261 cttttctcga tgccgttccg cccacatcgc cctccttgca tcctccgaag gaaagagtcc
     1321 cagtgcggaa cgagtttcga tatccacgcc ttcccagtac ccgcctaatg tcggaagatc
     1381 atgggtcgtg gctacggcaa gggattgagc gggataatga gccggaggct tccagcctcc
     1441 ccctggttct cgctcaaagt agaagactcg at
//
LOCUS       DASTWE010000700         1496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1602698, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000700 DASTWE010000000
VERSION     DASTWE010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602698"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFT30_07255"
     CDS             <1..515
                     /locus_tag="VFT30_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092464.1"
                     /translation="KPVGKKDLGLLANMYGHVYVARVAFGAKMLQTVQAFREAEAYHG
                     PSLIIAYSHCIAHGYDMAQGAAQQKLAVESGVWPLYRFDPRRIPNGEPPLNLDYGPPK
                     AKVADYMRNESRFRMIERADPARFKGFLEEAQAAAERRYLVYQQLSGMKVPAFDTKDG
                     NGNLDGEENK"
     gene            512..1489
                     /locus_tag="VFT30_07260"
     CDS             512..1489
                     /locus_tag="VFT30_07260"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEU5092465.1"
                     /translation="MNLATTYMGIPLSNPMVVGSSPLTGDLDTVRRLEDEGAAALVLP
                     SLYEEEITGDQMADFFSSESYVDSFPEATTYSPEPASAPGPDEYLEHLRRVKQAVSIP
                     VIASFNGSTPGGWMTYAHLMEEAGADAVELHLYHAASDPEMSSAQVESQMLQIIWEVK
                     GELRIPVAVKLSALFTAFANFALQIDAAGADALVLFTRFHKVDFDVIEFNVLRTVELS
                     NSSDLNLRLRGTAVLAGRVKASLAITGGVHTALDVIKGTMAGAHVVQLVSALMRNGPR
                     YLRSLRTEVEAWMLANEWTSLDEMRGNMSFQRIPNPAVYERETFRRMFQ"
BASE COUNT          349 a          395 c          404 g          348 t
ORIGIN      
        1 gaaaaccagt cggcaagaaa gacctcggac tgttggcaaa catgtacgga catgtctacg
       61 tcgcgcgtgt cgcttttgga gcaaagatgc ttcagacggt ccaggctttt cgcgaggccg
      121 aggcttacca cgggccgtcg ctgattatcg cctacagtca ttgcattgct catggttatg
      181 atatggccca gggcgcggcg caacagaaac tcgcagttga atcgggagtc tggccgctct
      241 accggttcga tccccgtcgc attccaaacg gagaaccacc gttgaacctc gactatggac
      301 cacccaaggc aaaagttgct gattacatgc gtaacgagtc gcgttttcga atgattgaac
      361 gcgccgatcc tgcgcgtttc aaaggctttc tcgaagaggc tcaagctgcc gccgaacggc
      421 gttacctcgt ctaccagcaa ctttcgggaa tgaaagtccc ggccttcgat acaaaagatg
      481 gaaatggaaa cctcgatgga gaggagaaca aatgaatctc gctactactt acatgggaat
      541 tccattgagt aatccgatgg tggtcggctc cagcccgctg actggagatc tcgatacagt
      601 gcggcggctc gaagatgaag gagcggccgc ccttgtgctc ccttctctct atgaagagga
      661 aatcacgggc gatcagatgg ccgacttctt ttcttccgaa tcctatgttg actcctttcc
      721 cgaggcaacc acttatagtc ccgagccggc atctgctcct ggaccagatg aatatctcga
      781 acacttacgt cgtgttaagc aggccgtcag cattcctgtg atcgcatcat tcaacggaag
      841 cactccgggc ggctggatga cttatgcaca cctgatggaa gaagctggag ctgatgccgt
      901 cgaacttcac ctctatcacg ccgccagtga tcccgaaatg agttccgctc aggttgagtc
      961 tcagatgctg caaattatct gggaagtgaa aggcgagctg cgtattccag tggcggttaa
     1021 gttgtcggct ctcttcaccg cttttgcgaa ctttgccttg cagatcgatg ccgccggggc
     1081 tgatgcgctc gtgctgttta cgcgctttca caaggtcgat ttcgatgtaa ttgagttcaa
     1141 cgtattgcgc accgtggaac tttcaaactc gtcggatctg aatcttcggt tacgtggaac
     1201 tgcggttctg gcaggacgcg tgaaggcgtc actggcaatc accggcggcg ttcacaccgc
     1261 tctcgacgtc atcaagggaa cgatggcagg cgcccatgtg gtgcaactgg tttcagctct
     1321 catgcgcaat ggaccacgat atttacgatc gctgcgaact gaagtggaag catggatgtt
     1381 agcgaacgaa tggacgtcgc tggacgagat gcgcggcaac atgagcttcc aaagaattcc
     1441 taatccagct gtttacgaac gcgagacctt cagacgaatg tttcagtaaa gccata
//
LOCUS       DASTWE010000701         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_827142, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000701 DASTWE010000000
VERSION     DASTWE010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827142"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFT30_07265"
     CDS             <1..206
                     /locus_tag="VFT30_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092466.1"
                     /translation="PEKIEINLRPTNKSDDFPVLWLGVLNDAMMPMLDIEWEILRPIN
                     AERIAFTAMVRFDRKETAVAESR"
     gene            complement(288..761)
                     /locus_tag="VFT30_07270"
     CDS             complement(288..761)
                     /locus_tag="VFT30_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092467.1"
                     /translation="MSKENYLYKGNVQIYLQGENALLPVASKTRGDQVAYFIGSKKSK
                     LLIRDFRREPIHAAHHARSGVEGAEFADAFNQPAYAEVSVSERGDEPEKIEINLRPTN
                     KSDDFPVLWLGVLNDAMMPMLDIEWEILRPINSERIAFTAMVRFDRKETAVAESR"
     gene            815..>1115
                     /locus_tag="VFT30_07275"
     CDS             815..>1115
                     /locus_tag="VFT30_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092468.1"
                     /translation="MFYSVKGKIKIYLEIFVCYNGTQPEKYFPMNLEHNTITTNGIRL
                     HVVQAGPKSGVPVVLVQGFPEFWYGYTQLTKRVGVDEEIHNFSFENYLKWIISSSK"
BASE COUNT          286 a          278 c          284 g          267 t
ORIGIN      
        1 aaccggaaaa gatcgagatc aacctgcgcc ccacaaacaa gagcgatgac ttccccgtgc
       61 tgtggctggg tgtgttgaat gacgccatga tgccgatgct cgacatcgag tgggagatcc
      121 tgcgcccgat caatgcagag agaattgcct tcacggcgat ggtgaggttt gacaggaaag
      181 agacggcggt tgccgagtca agataatccc gaccaagtcg cagagaatgc ttccagcgcc
      241 gtaccaagtg tacggcgctg gaagcattct ctgtgaccta gttgggatta tcttgactcg
      301 gcaaccgccg tctctttccg atcgaaccgc accatcgccg tgaaggcaat tctctccgaa
      361 ttgatcgggc gcaggatctc ccattcgatg tcgagcatcg gcatcatggc atcgttcagc
      421 acgcccagcc acaacacagg gaaatcatcg ctcttattgg tggggcgcag gttgatctcg
      481 atcttttccg gttcatcgcc tctttctgaa acagacactt cggcgtaagc cggctggttg
      541 aacgcatcgg caaactctgc gccttccacg ccggagcgag cgtggtgcgc ggcatggatc
      601 ggttcccggc gaaagtcccg gatcaggagc ttggacttct tcgagccgat gaaatacgca
      661 acctggtcgc cgcgagtctt ggaggcaacg ggcagaagag cattttcacc ttgtaaatag
      721 atttggacat tgcctttgta taagtagttt tccttggaca tgttatttct ccacaagatc
      781 ggttcaaaat aatacatcat tcaatgtaga acatatgttc tactctgtca agggcaagat
      841 taaaatttac ttagaaatct ttgtctgtta caatggcacg caaccggaaa agtacttccc
      901 catgaatttg gaacataata cgataacaac aaatggcatc cgcctgcatg tcgtgcaggc
      961 cggacccaaa agcggagtcc ccgtcgtgct tgtacagggc ttcccggagt tctggtatgg
     1021 gtacacacaa ctcacaaaaa gggttggtgt tgacgaagag attcataatt tctcttttga
     1081 aaattactta aaatggataa tatcaagctc gaaga
//
LOCUS       DASTWE010000702         1312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_406794, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000702 DASTWE010000000
VERSION     DASTWE010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406794"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFT30_07280"
     CDS             <1..626
                     /locus_tag="VFT30_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092469.1"
                     /translation="AEVPPNTCGVAACYEDQGDPDWLQFSVTINGITQSLLLGGNLSE
                     LIRSTDETLTGFDTLDFREEDVGVSFDQGITTFKFGVAAINLSEFNGGILAPGNSVPT
                     NFSWPAAPGSGFGSGVFSITHEIIDGNLGTDTFLHSVSAQFNLTSASAHIVAVPEPPT
                     ASIFLVGLVLLMLPGLRSRSAHADEIAKDAARHSLSARRIAGLVGLQ"
BASE COUNT          277 a          359 c          360 g          316 t
ORIGIN      
        1 ttgccgaggt tccgccgaac acttgcggtg ttgctgcgtg ctatgaggac cagggcgacc
       61 ccgactggct gcagttttct gtgacgatca acgggatcac tcagtctctg ctcctgggag
      121 gaaatctgag tgaattaatt cgcagtaccg acgaaacact taccggcttc gataccttgg
      181 acttcagaga ggaagacgtt ggagtcagct tcgaccaagg catcacaact tttaaattcg
      241 gagtcgctgc gatcaatcta tccgaattca acggtggcat tctggcgccc gggaactcag
      301 tccctaccaa tttctcttgg cccgcggcgc cgggttctgg ctttggttcc ggcgtcttca
      361 gcatcactca cgaaatcatc gatggaaatc tggggacgga tacgttcctg cacagtgttt
      421 cggcgcagtt caatcttacg agtgcttctg cacacattgt cgctgttcct gagccaccca
      481 ccgcctcgat tttcttagtg ggattggtat tgcttatgtt acccggcctt cggagccgat
      541 ctgcgcacgc tgatgagatc gccaaggacg ccgctcgaca cagtctctcc gcccggcgaa
      601 tcgccgggct tgtgggattg cagtaactac tagcctcttc agtcctgcgt ttagtacgtt
      661 aagatgactc tagtcggctg gggaaaatcg cagtcattac gctgcggtat gggcctggat
      721 catcgcccag gaggccgatc atgtatggtt tttacattgt tgcggcggtc attgttgtgc
      781 ttctcgtctt gatgctgaga cacagccggc tcttatagac cgggaagatc ctgatagcgc
      841 tgggtcacat ccagagcctg aattgccgga ccgaatatga atgcgctttg gctgagtcag
      901 gcgcccttgg gccgacacca tcactgcgag gatgacaacc gccacgacta gccaagcaca
      961 cttaactaga tcatggttgc gggcgtgatt gcccagcacc tcacgatagg agtcgaacgg
     1021 cagcatgacg ttcctcggga gaattcttaa tgtcgataag tggtcgctcg agccgatgct
     1081 gcggggagtt gctggacaag ttaggcgatc actgcaatcg ccacagccga cctaagaatc
     1141 gattccagta gtaggctgtg cgcctctatc acaccgatgt cactagcaga ggcgtagaac
     1201 ctcaactcca tcgcaccttg gacttcgccg ccatcaacgc gggcgaggga gcacatatga
     1261 cgaccatcta catcattgcg gcaatcgtag ttgtcttgat ggcgctgggt ca
//
LOCUS       DASTWE010000703         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_827173, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000703 DASTWE010000000
VERSION     DASTWE010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827173"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..599
                     /locus_tag="VFT30_07285"
     CDS             312..599
                     /locus_tag="VFT30_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5092470.1"
                     /translation="MAKSMTKSQIADYLAGKAGITKKGAVQILDDLAALAYREAKNVF
                     TVPGIGKLKLANRKARIGRNPQTGEEIKIPAKRVVKFRVAKAAKDSILGKK"
     gene            complement(730..1005)
                     /locus_tag="VFT30_07290"
     CDS             complement(730..1005)
                     /locus_tag="VFT30_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5092471.1"
                     /translation="MVTIVLTGQLQTVDGECDLACEIDGSMSVRQLIQRQGIQLRHLL
                     QLLREKKVLVTINKKIASEDSLVQDGDAIRLIGHDGMGGSGLGPSHH"
BASE COUNT          248 a          316 c          270 g          281 t
ORIGIN      
        1 tacaggcagg tctttaccca tgcactttca ccatggtcaa gagccgttta tgtcgacgtg
       61 cgaggtccat atagctctac tctacccttc catttgcttg aaccgcaagg ggtagtgtgt
      121 gcatttctcc acgccagacg cgctttttgt cgttgacacg cttcctgctc ttggctacac
      181 tctcgcgagt ttttacgatg tgagtacgac gaacacgctc accgtcgacc caaaccagtc
      241 agtgttttac acaaggcgcg ccaggcgaca tatcgactta cttcatccct taccctcaag
      301 gaggttcacg aatggcaaaa tcaatgacga aatcgcagat tgcagattat ctcgccggga
      361 aagcgggcat cacgaagaaa ggggcggttc agattcttga tgatctggcg gccttggctt
      421 atcgtgaagc caagaatgtc ttcacggtgc ccggtatcgg caagctcaag ctggctaacc
      481 gcaaggcgcg cattggccgg aatcctcaga ccggtgagga aatcaagatc ccggcaaagc
      541 gagtggtcaa gttccgtgtc gctaaggctg cgaaagactc gattctcggc aagaaataag
      601 gattatccca tcctgcacga tcgtagcggc tgatccatat cagttggact cattcgctgt
      661 ctttggaaga tacaagaggg ggtcgaggct ccacggagtc tcgacccctc tcttcatttg
      721 ccttcacttt tagtggtgtg aggggccaag cccactgcct cccatcccgt cgtgcccgat
      781 cagacgaatc gcgtcaccat cctggacgag cgagtcctcg ctggcgatct tcttattgat
      841 ggtcacgagc actttcttct cacgcaggag ctggagcaga tgtcgaagct gaattccttg
      901 tcgctgaatc agttggcgaa ccgacatcga cccatcgatt tcgcatgcca aatcacattc
      961 gccatccacg gtttgcaatt gtccggtcaa tacgatggtc accatttcct tcgttcctta
     1021 tacatgcaaa acgttctgaa cctctccctt ctacgctagt tgactcgtcc ggcatctctc
     1081 ctgataatgg atggccatgc catctctcga tgttc
//
LOCUS       DASTWE010000704         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_762924, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000704 DASTWE010000000
VERSION     DASTWE010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762924"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFT30_07295"
     CDS             <1..204
                     /locus_tag="VFT30_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092472.1"
                     /translation="GEATEELPARYEGEAINTGFNARYLLDVFSVMDGETISLQMETP
                     LSPCLIQESERPGFKCVVMPIKI"
     gene            387..>1148
                     /locus_tag="VFT30_07300"
     CDS             387..>1148
                     /locus_tag="VFT30_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092473.1"
                     /translation="MTDSSQPKSDSYSADQIKVLEGLDAVRKRPAMYIGSTGVDGLHH
                     LVYEVVDNSVDEHMAGFGEAIEVTIHIDGSVTVIDNGRGIPTGMHPTQKKSAAEVALT
                     VLHAGGKFEQGAYTVSGGLHGVGISVVNALSEWLELEIWQDAQVFEQRYERGKPNAPL
                     NAIGKTKRRGTKVRFKPDSQIFETLEFSFDVLAQRLRELAFLNKGLAITLRDERKEPA
                     KEQIFLYKGGIVSFVEHLNEAKTPLHKPIYVKVEKS"
BASE COUNT          280 a          314 c          333 g          221 t
ORIGIN      
        1 ggggaagcca cggaagaatt gcccgcccgg tatgagggag aggccatcaa tacaggattc
       61 aatgcccggt atcttctgga tgtcttcagt gtgatggatg gggaaaccat atcgcttcag
      121 atggaaaccc cgctgagccc ctgtttgatt caggaatcgg aaagaccggg gttcaagtgc
      181 gtggtcatgc ccattaagat ttagcgggat gttcaaaaag tccgccccca ccgctctcgc
      241 atcgaccgga ggcttaacgt accacagtgt acgtcccgcc tcttcgcggg tcgtcgcttg
      301 gccaggcgtc cttttcgagc actctgccga gcgtcccagg atcagacaag gcgaggctcg
      361 cttctcaacc gacggttagg aaacggatga ccgactcttc tcaacccaaa tcagacagct
      421 acagcgcgga tcagatcaag gtcctcgaag gtctcgatgc cgtacgaaag cgcccggcga
      481 tgtacatcgg gagcaccggc gtcgacggac tccaccatct tgtctatgaa gtcgtcgaca
      541 acagtgtcga cgaacatatg gcggggttcg gcgaggcgat cgaggttacg atccacatcg
      601 acggaagcgt gacggtcatc gacaatggac gtggcattcc caccggcatg catcccacgc
      661 aaaagaaatc ggccgccgaa gtggcgctga ccgtactcca tgcaggcggc aagttcgagc
      721 agggagccta caccgtctcc ggaggcttac acggggtcgg aatctccgtc gtgaacgcct
      781 tgtcggagtg gctcgagttg gagatctggc aggatgccca ggtattcgaa caacgatatg
      841 aacgcgggaa gcccaatgca cccctcaatg cgataggcaa gacaaaacga cgaggaacca
      901 aggttcgctt caagccggac agccaaatct tcgagacgtt ggaattcagc ttcgacgtgt
      961 tggcccagcg gcttcgcgag ttggcctttt tgaataaagg tctggccatt accctgagag
     1021 atgaacggaa agagccggcg aaagaacaga tcttcctgta caagggcggg atcgtgtcct
     1081 ttgttgaaca tctgaacgaa gccaaaacgc cgctgcacaa gcccatctat gtcaaggttg
     1141 agaagtcg
//
LOCUS       DASTWE010000705         1825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_51523, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000705 DASTWE010000000
VERSION     DASTWE010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51523"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..>1825)
                     /gene="dnaE"
                     /locus_tag="VFT30_07305"
     CDS             complement(33..>1825)
                     /gene="dnaE"
                     /locus_tag="VFT30_07305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5092474.1"
                     /translation="DFLGLKTLTMIRRAETLINETYPEAPPLVIDQVPFDDPTTFALL
                     SSGKTTGLFQLESSGMRDLLTGLKPDRFEDIIAIIALYRPGPMYLIPDFIKRKQGKVP
                     ITYEVPELEPLLKDTYGVIVYQEQVMAIANKMAGFSLGQADILRRAMGKKKPEEMEKL
                     RAKFLEGAKQKKIPEKKAEKLYELIQKFAGYGFNKSHAAAYAVVCYQTAYLKAHYPTE
                     FMASLMTTDMGNQDKLVGYFTECRDLGIKVLGPDVNASQKHFAVAGGAIRFGLAAIKN
                     VGEGAVESVLEVRTQGGLFGSFFDFCRRVDLHKVNKRMLEGLIKAGAFDSTHAKRAQL
                     MAVLDQAVEEGAAVQRERELGQISIFGEELNGHSTSTSLPTPQLPSLPEWDQAQRLKY
                     ERELTGFYISAHPLARYEATLSALSTTTTVGLKECSDGGEVKICGIIATVKSMLTKKG
                     DRMAYLTVEDLQGTTEVIVFPDLFRTAGELIAPERMVRITGSIDRGDKGTKIRGSKIE
                     PLAEVQAQSIKRIRIRLNDRPEVREQLPRLQEIFRRHPGRTSILMSFRTDGTLEAETA
                     PLPHLTVCASEHFVSDVEEVLGKGALSLLS"
BASE COUNT          388 a          531 c          489 g          417 t
ORIGIN      
        1 aacgatcggc caacagaccc cactaagacc ccctaagata gcaaagagag agcccctttg
       61 cctagcactt cctccacatc cgacacaaaa tgttcgctgg cgcagacggt gagatgaggg
      121 agaggagccg tctcggcttc caaggtgcca tcggttcgaa acgacatcaa gatcgaggtt
      181 ctgccgggat gcctccgaaa gatctcctgc aatcgaggca actgctcacg gacctccggt
      241 cggtcattga ggcggatacg gattcgtttg atggactgcg cctggacctc ggctaagggt
      301 tcgatcttac taccccgaat cttggtgccc ttgtctcctc gatcaatgct gccggtgata
      361 cggaccatcc gctccggtgc gatcagctca ccggcagtcc tgaacaaatc tggaaatacg
      421 atcacttcag tggtcccttg cagatcttcc accgtgagat aggccattcg atcgcccttc
      481 ttcgtcaaca tcgatttgac cgtggcgatg atgccgcaga tcttcacctc gccgccgtcg
      541 gagcattcct tcaatcccac cgtcgtcgtg gtcgacaagg cactcagcgt cgcttcgtag
      601 cgggcaaggg gatgagcgga aatatagaac ccggtcagtt cccgttcgta cttcaatcgt
      661 tgcgcttgat cccattccgg gagcgaaggc agctgggggg tcggcaagga cgtcgaggtg
      721 ctgtgcccat tcagctcctc tccgaaaatg ctgatttgcc ccagctcacg ctctcgttga
      781 acagccgcgc cctcttccac ggcttgatcg agcaccgcca tcagttgcgc ccgcttggca
      841 tgagtcgaat cgaaggcgcc agccttaatc aggccttcca acatccgctt attcaccttg
      901 tgaagatcga cccgccggca gaagtcaaaa aacgatccga agagaccgcc ctgagtccgc
      961 acttcgagaa ccgactccac ggcaccctcg cccacgttct ttatggctgc caagccgaac
     1021 cggattgccc cgccggcaac tgcgaagtgt ttttggctgg cgttgacatc gggtcccagg
     1081 actttgatgc ccagatcccg gcattccgtg aaatagccga ccagtttgtc ctggttgccc
     1141 atatcggtcg tcatgagaga cgccataaac tcggtcgggt agtgcgcttt gagataggcc
     1201 gtctgataac agacgacggc ataggcggca gcgtgcgatt tattgaaacc gtatccggcg
     1261 aatttctgga tcaactcgta gagcttctcg gctttcttct cgggaatctt cttctgcttc
     1321 gcgccttcga ggaacttggc ccgcaacttt tccatctctt ccggtttctt tttacccatc
     1381 gcacgacgaa gaatatcggc ttggcccaac gagaaaccgg ccatcttgtt agcgatcgcc
     1441 atgacctgtt cctggtagac gatcacacca taggtatcct tgaggagcgg ttccagttcg
     1501 ggaacctcat aggtgattgg aaccttgccc tgctttcgct tgatgaaatc aggaatgagg
     1561 tacatcggtc cagggcgata gagcgcgata atggcaatga tatcttcgaa ccggtcgggc
     1621 ttcagtccgg tgagcagatc gcgcatgccg gaactttcaa gctggaacag tcccgttgtt
     1681 tttcctgagc tcaagagagc aaaggtggtc ggatcatcga acggcacctg atcgatcacc
     1741 agtggcggcg cctcgggata tgtttcgttg atgagagtct cagctcgtcg gatcatcgtg
     1801 agggtcttga ggccgagaaa gtcga
//
LOCUS       DASTWE010000706         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_245831, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000706 DASTWE010000000
VERSION     DASTWE010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245831"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1611..1799)
                     /locus_tag="VFT30_07310"
     CDS             complement(1611..1799)
                     /locus_tag="VFT30_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092475.1"
                     /translation="MPKLHAMTDGAKVGDAPKVVMPPKVNSWPKVAFVVWYPKVVGLP
                     SQGMVTLGCRRKLLVGKR"
BASE COUNT          522 a          637 c          478 g          431 t
ORIGIN      
        1 ttttagcact tatcctcgtt agaggttcat ccttagagag agaaacgatt cttcggcccc
       61 attaaccccg gaggcactcc tcctccgtcc cctatggcag cgccaccgcg gccggtcgcg
      121 ctactagcca tatttcctct ctctacacga gggagaacat catgtcgaga tggctcctgc
      181 cttcgtggac cgttcccgcc cgtcgtagct gattttgttc atccgatttt cactagatcg
      241 aagttgtgat tcgcttgtgc agcgttttgg gtgctttttg tgtgtgcgtc aacttacaaa
      301 gtgccccgat cgcccaccga tcgtcttttc tgtgcttcgt gccaacggcc gttttctcaa
      361 gatggcgtcg ccgaacaaag aacctccggc tcgccaagat ctcccacagc catggtcgaa
      421 aaccggcaga gtctgtactc acatcagaag tgaacgtgat ccgcaccctt tttgtcgttc
      481 ttgcagggga tgcaagatca tcgaagaccc ttgcacggat tgcgtgaaac tcaattctaa
      541 acaaatcaat gaactcgccg aactggctcg aaagagtgaa atacgtaaag aacaagaacg
      601 ccgcaaacgg gctcgacaac ggaacgtttc ggcttctcaa gtgtctaccc cgagttcgac
      661 gttttccaca ccgggtaaag taaggaaaac gaccaagaag cgttcgccca aaacgaagac
      721 gaagcaccca aagggcatca cactgccatc gtcttccaag agatcttgta cccagatccc
      781 ggatctatcc gattcgatga ccgggaagga catcaaccca acgaaggaaa ttccatccac
      841 cttcctcgtg atatcggaaa cggatgacac cttcgccgaa cagaacgaag gtgaatcttc
      901 accttcacta catgaacaac ttctcccaac gcagcaaggc gtgccttcgg acaccaggga
      961 gatggccgac gaacaggagg gctcaccctc atcgctctca ctttccacag gcctggatca
     1021 cgatccacat tatgacttaa cggacaagat aacgcagatc cttaaggatg ttctaccaag
     1081 atatttgcca caaagaacat cggatcaggc ctttcgggat ggatctatcc ccggggttgt
     1141 cccggcaaga gtcggtaaga caagcaccga cgccgttacg aacctcattc atgaagaagt
     1201 gcttctggcc cgcacggcca cactcgccgc gggattgacc ctgccgcaac tctgcccacg
     1261 acgggaggag atcggccaaa cagtcgacga cgcgccggct agcccggatc cgccatcgtc
     1321 gcaacaaccc gctgacgtcc cgtgcacacc ttcgcttata cgagacaaca ctcgccttcg
     1381 tccggcgtgg aggatttaca aggcgccggt atcggaacgt gcatacgagg ctgagaagcc
     1441 atcaagaatg actagaaaat taaacccgtt tgccgaccct cgaaaacgtc ttctctctga
     1501 gacagatgaa tcgataagca aaagacagag aatgaacgtc ttcaaaaacg aatttggtgc
     1561 aacactatat gatcattcgg acctaccgtg gccggatcaa gccacaaatg tcaccgtttc
     1621 ccaaccagta gttttcgtcg acaacccaag gtcaccatcc cctgagaagg aagaccaact
     1681 accttcgggt accaaaccac gaatgccacc ttcggccaag agtttacctt cggaggcatc
     1741 actaccttcg gcgcatcgcc caccttcgca ccatctgtca tcgcatggag cttcggcatc
     1801 caaaccacct tcgctcagtg cagcggcgtc cccggaaggc cagtgctcac cttcgccggt
     1861 aggctcgcct tcgcaccact caccttcgct ttcccaagca ccttcgccca gcagggtgcc
     1921 ttcgcacctt cactccccgg gcgtctcacc aggcaaggga ttaatcccac cctcgtcggg
     1981 tctctccata caagaccatc aacggtttgc caatcgccgg gtcacctggg atgattcgaa
     2041 gagtgacgaa gcaaaaagtg aatcatgg
//
LOCUS       DASTWE010000707         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1150495, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000707 DASTWE010000000
VERSION     DASTWE010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150495"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFT30_07315"
     CDS             complement(<1..866)
                     /locus_tag="VFT30_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092476.1"
                     /translation="MSRLNLLRYGIPILLFIGCNRPIASSPTPDLFATLQASTPSGLA
                     SPVATPNATSGGSTGGFTVPLPSTGDELTGHIVFTCQLFKVQAMDQICIMNADGSGMR
                     RLTTENNKRHFYPALAPDGQSVLYTSFREQNVYEIYSFDLNDESVDQLTDRLGVLTAP
                     EVSPDGTSITFARGNPSSGSYQIMVMDRDGGNVDNIPQVAGWDPTWSPDGQQILFASG
                     AEGAAQLFVVKLNGSDLHRVSNLPAIRGRSDWSADGQSIVTYSGPAWNREVYIMNADG
                     LNARQLTPSGGN"
     gene            complement(853..>1134)
                     /locus_tag="VFT30_07320"
     CDS             complement(853..>1134)
                     /locus_tag="VFT30_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEU5092477.1"
                     /translation="GVRSLRIALGEIAEIDTASIQKKWDELSKGTPVERAPLHFRFIK
                     AEVQCMACFQKYHPSDGRIHCPYCGSYGAKVLSGEEFFLESIEFDDEQT"
BASE COUNT          264 a          299 c          312 g          259 t
ORIGIN      
        1 ctgtttcccc cgctcggtgt gagctggcgg gcattcaacc cgtcggcgtt catgatgtac
       61 acttcacgat tccacgccgg acccgagtat gtcacgatgg actgtccatc cgctgaccaa
      121 tcactgcggc cgcggatggc aggcaggttc gacacccgat gcaaatcgct gccattaagt
      181 ttgacaacaa acagctgcgc cgctccctcg gcacccgagg caaacaggat ttgttgtcca
      241 tccggcgacc aggtgggatc ccaaccggca acctggggaa tgttatcgac atttccgccg
      301 tcccgatcca tgaccatgat ttgataactg ccgctgctgg gattccctcg ggcaaaagtg
      361 atggacgttc catccggtga gacctcgggc gcggttaata cgccgagtct gtccgtcagt
      421 tggtccacac tttcatcgtt gaggtcgaaa ctgtagatct catacacgtt ttgttcgcgg
      481 aaggaggtat acaggacgct ctgaccatca ggggcaagag ctggatagaa atgacgcttg
      541 ttgttctcag tggttagcct gcgcatcccg gaaccgtcgg cgttcataat gcagatctgg
      601 tccattgcct gcactttgaa gagctggcag gtgaaaacaa tatgaccggt aagctcgtcc
      661 ccggtagagg gtaaaggaac tgtgaatcct ccggtggaac cacctgatgt agcgttgggt
      721 gtggctactg gagaggcaag ccccgatgga gtggaggctt gcagcgtggc gaacaggtca
      781 ggggtggggg aagatgcaat cgggcggtta catccgataa acaataaaat cggtattccg
      841 tagcgtaaaa ggttaagtct gctcatcgtc aaattcaatc gactcaagga aaaattcttc
      901 accggataaa actttcgcac cataactgcc gcagtaaggg caatggattc gtccgtccga
      961 cggatggtat ttctgaaagc acgccataca ctgcacttct gctttaatga aacgaaaatg
     1021 gagcggggcg cgttctactg gcgtgccctt gctcaattcg tcccattttt tttggatgga
     1081 agccgtgtcg atttcagcaa tctccccaag agcaatccga agactccgca cgcc
//
LOCUS       DASTWE010000708         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_19504, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000708 DASTWE010000000
VERSION     DASTWE010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19504"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..380)
                     /locus_tag="VFT30_07325"
     CDS             complement(51..380)
                     /locus_tag="VFT30_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEU5092478.1"
                     /translation="MRLNANVRPAKSPHLTIEQLWNIGPTVARRLKEIGIHTEADLRA
                     VGSVAAYQRIRAKHPGKTVPVCYYLYSLEGALRNQHWDTLGSTIKQNLLRQVSSGKGV
                     QRKRILK"
     gene            complement(520..924)
                     /locus_tag="VFT30_07330"
     CDS             complement(520..924)
                     /locus_tag="VFT30_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5092479.1"
                     /translation="MQSATRSSSVSNKQLWLGRTMSAMPALFLLVDGAMKLVKPEVVI
                     KATVELGYAESVIVPLGIVLLTCTILYLVPRTAVLGAILLTGYLGGAVATHVRAGQGL
                     FEILFPVVFGALLWGGLMLRDERLRELLPLRD"
     gene            complement(1022..>1377)
                     /locus_tag="VFT30_07335"
     CDS             complement(1022..>1377)
                     /locus_tag="VFT30_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5092480.1"
                     /translation="PVIHFEIMGGKGNQLEKFYGELFGWKIDSNNPMKYGIVDTKGGP
                     GGINGGVGTSQDGNKRVSVYVQVDDLQATLNKVEKLGGKTILPPTEVPGGPKLAMFTD
                     PAGNVTGLLLGGLAG"
BASE COUNT          305 a          393 c          348 g          331 t
ORIGIN      
        1 tttaatgatc tgcatttgac cggtaattcc tcactggcga ggtgggcgcc ttatttcaat
       61 atgcgcttcc gttgcacacc cttgcccgaa gatacttggc gcagcagatt ctgtttgatc
      121 gtcgaaccga gggtatccca atgttgattt ctcaatgcgc cttcaagcga gtacaggtag
      181 taacagaccg gcaccgtctt ccccggatgc ttggcacgga tacgctgata ggctgcgacg
      241 gatcctactg ctcgaaggtc ggcctcggtg tgaatgccga tttccttgag tcgacgcgca
      301 acggtcggac cgatgttcca aagttgttcg atcgtcaggt gtggtgattt cgctggtctg
      361 acgtttgcat ttaatcgcat gagcctctct agtctcctat gatgctgcat actcgtccgg
      421 accaatctat tgccttggaa tccgtcgttc aagagaaaac tctagagagc agcgctctta
      481 aatcagcaga aacccgttag agtctcgttg ctggatgtgc taatcccgta agggaagaag
      541 ctcgcgcagg cgttcatcac gcaacataag tcctccccag agcagggcgc cgaatacgac
      601 gggaaacaaa atctcgaaca gtccttgtcc ggcgcgtaca tgagtcgcta cggcaccacc
      661 taaatagccc gttaacagga tggcaccgag aaccgcagtc cgtggaacca gatagaggat
      721 ggtacatgtc agcagtacga ttcccagagg gacaatcaca gactcggcgt atccaagttc
      781 cacagttgcc ttgataacca cctctggttt caccaatttc atcgccccgt ccacgagcag
      841 gaataatgcc ggcatggcgc tcatggttcg ccccagccag agttgcttat ttgagacgga
      901 agatgatcga gtcgctgatt gcatggaaat cctccaagcc tggataccat cccgcttcgc
      961 tggtcagcat cctgggtcca gcaagagacc tcaagtggtc tgataacttc ctgagaatcc
     1021 tctagccggc taacccaccg agaagaagtc cggtcacatt gcccgccgga tcggtaaaca
     1081 tggccagctt ggggccgccc ggcacttcgg tcggcggcag aatcgtcttg ccgccaagct
     1141 tctcgacttt attcaaggtc gcttggagat cgtctacttg gacgtagacc gagacccgtt
     1201 tgttgccgtc ttgactcgtg cctacgccgc cattgatccc tcccggtccg cccttcgtat
     1261 cgacgattcc gtacttcatc gggttgttgc tgtcgatctt ccacccaaag agctcaccat
     1321 agaacttctc gagctgatta cccttacctc ccatgatctc gaaatggatg actggtg
//
LOCUS       DASTWE010000709         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_665742, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000709 DASTWE010000000
VERSION     DASTWE010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665742"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..450
                     /locus_tag="VFT30_07340"
     CDS             115..450
                     /locus_tag="VFT30_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5092481.1"
                     /translation="MDITVQEFKHCDMITVKGRVDSATAPQLAQALESANDGGKYKLV
                     VNMSELEYMSSAGFRALLAAQRNCKRYNRGEVVLASVPERIREALELAGFTELFKTFD
                     DPLQAVGHF"
     gene            539..949
                     /locus_tag="VFT30_07345"
     CDS             539..949
                     /locus_tag="VFT30_07345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092482.1"
                     /translation="MKTVQFAAKFEYLDEIREFVGTIARDGGFGDKDVYNIQLATDEA
                     ASNIIEHAYEKITDGVLELSCGVHNDRITIVLIDHGESFDPSEIPLPDLKADLSDRKI
                     GGLGIFLMRKLMDEVHYEVKPNKSNVLTMTKRKR"
BASE COUNT          264 a          266 c          291 g          286 t
ORIGIN      
        1 taacattttg tcctcgaagt tagacactcc cccattgtga agcaaagctg gctgatcagt
       61 atctcatgat ataattttat gcatcagcac agtactttca cttggaggag ttctatggac
      121 atcacggttc aagagttcaa gcattgcgac atgatcacgg tcaaggggcg cgtggatagt
      181 gcgacagctc cgcagctggc acaggccctt gaaagcgcaa acgatggtgg caaatacaag
      241 ctcgttgtca acatgagtga gttggaatac atgtccagtg ccggcttccg tgccctcttg
      301 gctgcccaac gcaactgtaa gcgttacaat cgtggagaag ttgtcctggc ctcggttccc
      361 gagcgaatcc gcgaggctct ggaactggct ggttttaccg aactcttcaa gacctttgat
      421 gatcctctcc aagctgtagg ccatttttag gcttttgtct gccaggtgga gtcataaata
      481 ttataaaagg aggccgcctt tagttgtgtt gcactgaggc ggctcttaac tttgccttat
      541 gaagactgtt caatttgccg cgaaattcga atatcttgac gagatccgtg aatttgtcgg
      601 tacgattgcg cgtgatggcg gttttggtga caaggatgtg tacaatatcc agcttgccac
      661 cgatgaagct gcatcgaata tcatcgagca tgcctacgag aaaatcaccg atggggtgtt
      721 ggaattatcc tgtggggtgc ataacgatcg catcaccatt gtcctgatcg atcacggcga
      781 atcgttcgat ccctctgaga tcccgctccc tgatctgaag gcggatctct ccgatcgcaa
      841 gatcggcggg ctgggcattt tcctcatgcg caaattgatg gatgaagttc attacgaggt
      901 aaaaccgaac aaaagcaatg tgctgacgat gaccaaacgc aagaggtaag tatggcgtct
      961 ggttcacggt ctcctcgctc ttcagattgg cgcgacatcg ccagcctggg ggaacagatc
     1021 gtcaattctg attctctggc ggaacagcgc gaccacatcg tcgtcatgac gtccaaactg
     1081 attaccgggg atgtggacgt ctggctt
//
LOCUS       DASTWE010000710         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1538229, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000710 DASTWE010000000
VERSION     DASTWE010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538229"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /gene="rplD"
                     /locus_tag="VFT30_07350"
     CDS             complement(<1..479)
                     /gene="rplD"
                     /locus_tag="VFT30_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU5092483.1"
                     /translation="MPTIDLVDLQKKKVGTVDLSPQVFGCEPRAALVHEAVVMQRACE
                     RRGTASTLRRGEVSGSGKKPWTQKHTGRARAGSLRSPVWRHGGSVFGPKPRSYAYSMP
                     KKKYRIALQSALSAKVAESKLFIVSDLSLQQPRTKLLAQALKQFTGGDHALVVVARE"
     gene            complement(481..1107)
                     /gene="rplC"
                     /locus_tag="VFT30_07355"
     CDS             complement(481..1107)
                     /gene="rplC"
                     /locus_tag="VFT30_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU5092484.1"
                     /translation="MDMTNGLIGKKLGMTQVFDESRLTPVTVIEAGPCRVVTVRTKER
                     DQYEAVQLSFGEVKERKLSMPELGHLKKNQAPPSRVLREFKKDGEPTVGQLVTVSMFK
                     KGDWVDVIGVSKGKGFQGVVKRHHYAGGPESHGSMFHRAPGSIGASSFPSRVWKGKTL
                     PGHMGSERVTVQRLKVIESRSEENLLFVRGAVPGAANGIVVVRKSKKS"
     gene            complement(1164..1475)
                     /gene="rpsJ"
                     /locus_tag="VFT30_07360"
     CDS             complement(1164..1475)
                     /gene="rpsJ"
                     /locus_tag="VFT30_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU5092485.1"
                     /translation="MKVDQRIRIRLRGFDYRVLDQSVTEIVETVRRSGARVVGPIPLP
                     TRIEKITVQRSTHADKKSREQFEMRTHKRLLDIMEPTPETMDSLMKLNLAAGVDVEIK
                     L"
     gene            complement(1516..>2630)
                     /gene="tuf"
                     /locus_tag="VFT30_07365"
     CDS             complement(1516..>2630)
                     /gene="tuf"
                     /locus_tag="VFT30_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5092486.1"
                     /translation="LTKVCADKGMAKFVSYDEVAKASESQGRRDATKIMTIAISHVEY
                     ETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGV
                     PYIVVFLNKADKVDDKELLELVELEVRELLTKYGFPGDKTPIIHGSALKAMEADQGEL
                     GVPSILKLLEAVDTYIPTPQRPIDKPFLMPIEDVFTISGRGTVVTGRCERGIVKVGDE
                     IEIVGLRPTQTTVVTGVEMFRKVLDEGQAGDNIGVLLRGTKKEDVERGMVLCKGKSIT
                     PHTKFKAEIYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVQLNPGVEMVMPGDNVS
                     VTAELISPIAMDQGLRFAVREGGKTVGSGVVTEILA"
BASE COUNT          537 a          900 c          587 g          606 t
ORIGIN      
        1 tgttccctcg cgacaaccac cagagcgtga tctcccccgg tgaattgctt taacgcttgc
       61 gccaataact tcgtcctggg ctgctgcaga gaaagatcgg acacgataaa caatttgctc
      121 tccgccactt tagccgacaa ggcgctttgc agcgcgatac gatacttttt cttcggcatg
      181 gaataggcat agcttcgcgg cttcggtcca aaaacactcc ctccatgacg ccagacagga
      241 gaacgaaggg acccggccct tgcacgtccc gtatgctttt gcgtccatgg ctttttaccg
      301 gacccgctca cttcacctcg ccgcaacgta gacgccgtcc ctctgcgctc gcaggctcgt
      361 tgcataacga ccgcttcatg caccaacgcg gcgcgaggtt cacagccgaa tacttgcgga
      421 gacaaatcga cggttcccac cttctttttc tgcaaatcga ctagatcgat agtaggcata
      481 ctagctcttc ttcgactttc gcacgacaac gatgccatta gcggccccgg gaacagctcc
      541 acggacaaag aggagatttt cttcagatct cgactcgatg actttcaacc gctggaccgt
      601 gacccgttcc gaccccatat ggcctggcag agtcttccct ttccacacgc gagaaggaaa
      661 cgaactagca ccgatagatc ctggcgcccg atgaaacatg gacccatgcg attcagggcc
      721 accggcataa tgatgtcgct tgacaacacc ttgaaatcct ttacccttcg atacgccgat
      781 cacgtcgacc caatcgccct tcttgaacat actcaccgtc accagctgcc caacagttgg
      841 ctctccgtct tttttgaact cacgcaacac gcgactgggc ggggcttgat ttttcttcag
      901 atgtcctaat tccggcatcg acagcttgcg ctccttgact tcaccaaatg acagctgaac
      961 cgcttcgtac tgatccctct ccttagtcct cactgtcacg acgcggcagg ggccagcctc
     1021 aatcaccgtc accggcgtca aacgactctc atcaaaaact tgagtcatgc cgagcttttt
     1081 cccaatcagt ccgtttgtca tatccatcca gtaccaaacg tttacgtctc gactactgag
     1141 ttcacaaact tagcactcaa gactcataac tttatttcga cgtccactcc cgctgccaga
     1201 ttcagcttca tcaacgaatc catggtctca ggcgtgggct ccatgatgtc gagcaatcgc
     1261 ttgtgtgtac gcatctcaaa ttgctcgcga gatttcttgt cagcgtgcgt cgaccgctgc
     1321 acggtaattt tttcaatcct cgtggggagc ggaattggac ctaccactct agctccgctg
     1381 cgtcgaacag tctcgacaat ttccgtgacc gactgatcca gcactcggta gtcaaagccc
     1441 ctcaatctga ttctgatccg ctgatcgact ttcacttcat tcactcctaa gctgccaagc
     1501 agtcaatgtc tctacctatg ccaggatttc ggtgacgacg ccggagccca ccgtcttgcc
     1561 gccctcgcgc acggcaaacc gcaacccctg atccatcgcg atcggactga tcaactccgc
     1621 cgtcacactc acgttgtcgc ccggcatcac catctccacc ccgggattca actgcaccac
     1681 ccccgtcaca tccgtcgtcc ggaagtagaa ctgcggccga tacccgttga agaacggcgt
     1741 atgccgcccc ccttcttcct tcgtcaacac atagatctcc gccttgaact tcgtatgcgg
     1801 cgtgatgctc ttccctttgc acaacaccat cccgcgctcg acgtcttcct tcttcgtgcc
     1861 ccgcaacagc acgccgatgt tgtcccccgc ctgcccctca tcgagcactt tgcggaacat
     1921 ctcgacgccc gtcaccaccg tggtctgcgt cggccgcaag cccacgatct cgatttcatc
     1981 gcccactttc acgatgcccc gctcacagcg ccccgtcacc accgtcccgc ggccgctgat
     2041 ggtaaacacg tcttcgatcg gcatgagaaa gggcttgtca atcggccgct gcggcgtcgg
     2101 aatgtacgta tccaccgcct ccaacagctt caggatggag ggcactccca attcgccctg
     2161 atccgcctcc atcgccttca acgcactgcc atggatgatg ggcgtcttgt cccccgggaa
     2221 cccgtatttc gtgagcaact cccgcacttc caactccacg agctccaaga gctctttgtc
     2281 atcgactttg tcggccttgt tcaagaacac gacgatgtac ggcacgccca cttgccgggc
     2341 caagagaatg tgctcgcggg tctgcggcat ggggccgtcc gccgcactga cgacgagaat
     2401 cgccccgtcc atctgcgccg ccccggtaat catgttcttc acgtagtcgg cgtggcccgg
     2461 gcaatcgacg tgggcataat gccgattggc cgtctcatat tccacgtggc tgatggcgat
     2521 ggtcatgatc ttcgtcgcat cccgccgccc ttggctctca ctcgccttcg ccacttcgtc
     2581 atagctcacg aacttcgcca tccctttgtc cgcacacact ttcgtcaacg
//
LOCUS       DASTWE010000711         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_245979, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000711 DASTWE010000000
VERSION     DASTWE010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245979"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VFT30_07370"
     CDS             <1..560
                     /locus_tag="VFT30_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5092487.1"
                     /translation="GETRNQRFQPFRDLVIEEESAGQKKPYHDPMTLRGLNHAMGFPY
                     DNMATLGQFIFTGILERFPKLRLAILESNAGWAPFWLGRMDVHTHGRHGVMGKPEHLS
                     MLPSEYFKRQCTVACDSDEAALKYVVDFLNGENIVWNTDYPHADGMEPARALPDFDAH
                     PISDEAKRKILWDNAVTLYGQRLIS"
     gene            621..>1610
                     /locus_tag="VFT30_07375"
     CDS             621..>1610
                     /locus_tag="VFT30_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5092488.1"
                     /translation="MADYKLISADSHVNEVAATWEQVRRKYGERAPEIVWNPSEREHG
                     PYLSIRDWETSLEGKNRESCAMEFVGMVIGGFGVGSVVGRTSAKAGEFRKNFRFEDWA
                     GPWEPNARLKDMDRDGVEVDILYASHLRHIYGLSIKDEPFFRAIAQSYNDWLMEYCSV
                     APNRLVGLPVLSILNIDGAVEDLTIYAKQGAKGFMIASSTPIGMNYGESKFNSLWAAA
                     QDADVPLCLHTTTGAWKKAKFHHPIIRTFIRGEGEIQTSLLEMIYGGVFDRFPRLKIV
                     AAEWDIGWVAHMVAKLKDHDPKTGLKLAPADYFRRNIWFTFENDRAGVLTTPLY"
BASE COUNT          386 a          411 c          469 g          344 t
ORIGIN      
        1 acggggaaac cagaaatcag cgctttcagc cgtttcgaga tctggtcatc gaagaggagt
       61 cagccgggca aaagaagccg taccatgacc cgatgacgct tcgaggactc aatcatgcga
      121 tgggatttcc gtacgacaac atggctaccc ttgggcagtt tatcttcacc ggcattttgg
      181 agcgctttcc caagctcagg ctggcaatac tggaatcgaa tgccggctgg gctccctttt
      241 ggctcggccg aatggatgtg cacacgcacg gccgccatgg cgtgatggga aaaccggagc
      301 atctgtccat gctcccgagc gaatatttca agcggcaatg tactgtcgcc tgcgacagtg
      361 atgaagcggc gctaaagtac gtcgtggact ttctaaatgg agaaaacatc gtgtggaata
      421 ccgactatcc gcatgcggac ggaatggaac cagcgagagc tcttcctgat ttcgatgcgc
      481 atccaatctc cgacgaagcc aagcgcaaga tcctgtggga caacgcggtc acgctctatg
      541 gacagcgctt gataagctaa cttgataggt taatgagttg aggaagagag taaacgacta
      601 accgaatcgg gaggcgccca atggccgatt acaaactgat ctcggcagat tcccacgtca
      661 acgaagtcgc ggcgacctgg gagcaggtac gccggaagta cggcgagcgg gctccggaga
      721 tcgtgtggaa tcccagcgaa cgggaacacg gtccttatct gtcgattcgg gactgggaaa
      781 caagcctcga ggggaaaaac cgcgagtcct gcgccatgga gtttgtcggt atggtgatcg
      841 gcgggtttgg tgtcggcagc gtcgttgggc gaacttcggc aaaagcaggc gaatttcgca
      901 agaactttcg cttcgaagac tgggctggac cgtgggagcc gaacgcgcgc ctgaaggata
      961 tggaccgtga cggcgttgaa gtcgatatct tatatgcgag tcacctgcgc cacatttatg
     1021 gtttaagcat aaaagacgag ccgttttttc gggccatcgc tcagtcctat aacgactggc
     1081 ttatggaata ctgcagcgtg gcgccgaacc ggctcgtcgg tctccctgtt ctttccattc
     1141 tgaacatcga cggtgcggtc gaagatctga caatttacgc gaagcaaggg gcgaagggat
     1201 tcatgatcgc atcgtcaacg ccgatcggca tgaactacgg tgaatccaag ttcaattcgt
     1261 tgtgggccgc tgcgcaggat gctgatgtgc cgctctgcct tcacacaacc acaggcgcat
     1321 ggaagaaagc gaagtttcac catccgataa ttcgtacatt cattcgcggc gaaggagaaa
     1381 ttcagacgtc tcttctagag atgatctacg gcggcgtttt cgatagattt cctcggttaa
     1441 aaattgtcgc agccgagtgg gacatcggct gggtcgccca catggttgct aaactcaagg
     1501 accacgatcc gaagaccgga ctcaagctcg cgcctgcgga ttactttcgc aggaacatct
     1561 ggttcacatt cgagaatgac cgggccgggg ttttgacgac gccgctctac
//
LOCUS       DASTWE010000712         1455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_181028, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000712 DASTWE010000000
VERSION     DASTWE010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181028"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFT30_07380"
     CDS             complement(<1..777)
                     /locus_tag="VFT30_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5092489.1"
                     /translation="MKQPFPRGLWLAGCVSIILASGLPAGAGAYESRTVEDGAVVRGK
                     VTFTGTVPDPKEFELRRSPDREFCSAVSDGNGHRLLKEVNIGTDGGLKDVVVVVEGVE
                     RGKPFTFTDAEVEASLCQFLPFVTVVSDKRRVTVFNRDPVLHDIQGYAYDETGVDIVL
                     HRPALHESGTTDIVRLVKGRKVFTMQCGMHPYMQNWGYAIDNPYYAVTDLNGSFVIDD
                     LPAGTYHIKAWHPILGMQERNITVRPNETVPLDWLFDAKRV"
     gene            complement(797..1084)
                     /locus_tag="VFT30_07385"
     CDS             complement(797..1084)
                     /locus_tag="VFT30_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092490.1"
                     /translation="MSEKMEGEILREFVGDETLNGYLTELFGVTVAGNGQTHQYYRWV
                     TKAERFPFNTVSKQGKWSEEFRRRIFTEQSPLLFELPQRVDPANQSAGTQH"
BASE COUNT          301 a          449 c          351 g          354 t
ORIGIN      
        1 cactcgtttt gcgtcaaaca gccaatcaag cgggaccgtc tcgttcggtc tcaccgtgat
       61 attccgctcc tgcattccta aaatcggatg ccaggccttg atatggtacg tgccggcggg
      121 gaggtcatcg ataacgaacg atccattgag gtccgtgacc gcataatacg gattgtcgat
      181 cgcatacccc caattctgca tgtagggatg catgccgcac tgcatggtaa acactttccg
      241 ccctttgacc aatcgtacga tatcagtggt tccgctctca tgcagcgcag gccgatggag
      301 gacaatatcg acccctgttt catcgtacgc atacccttga atatcgtgta aaaccggatc
      361 tcgattgaac acggtgactc ggcgcttgtc gctgaccacg gtgacgaacg gcaggaactg
      421 acaaaggctt gcttccactt cagcatcggt gaaggtgaac ggcttgcctc tctccacgcc
      481 ttccacgacc accactacat ctttgagccc gccgtccgtc ccgatattga cttccttcag
      541 gagccgatgc ccatttccat ccgacacagc gctgcaaaac tcgcggtccg gggaccgacg
      601 cagctcgaac tctttcggat ccggcacagt tccggtgaac gtgaccttgc cccgcacgac
      661 cgcaccatcc tccacggtcc ttgattcata cgccccggca cctgctggaa gtcccgatgc
      721 caggataatc gacacacagc cggccagcca cagtcctctt ggaaacggtt gtttcatatc
      781 ccctccgctc gcatcctcaa tgttgcgtac ctgctgattg attggccgga tccactcgct
      841 gcggaagctc gaacaacaat ggcgactgct cggtaaagat ccgccggcga aattcttccg
      901 accacttccc ctgcttgctc actgtattga atggaaaccg ttcggccttg gttacccatc
      961 gatagtattg gtgagtttgt ccgttccctg caactgtgac cccgaacaac tccgtgagat
     1021 aaccgttcaa tgtttcgtcc cctacgaact cccgcaagat ttctccttcc atcttctcgc
     1081 tcattgtcaa tcgatgatcg cttgtaatcg gttcctcgac atactgccgc cgcctcgatt
     1141 agccaggcgg tctcgagatc cgctcgtacg atgatcacgc cggacagttc cacacggatc
     1201 agtacggtgc gcaaaggctt catagcactt ggcgtgacgc tggcgagtta tgatttctgc
     1261 ccacgccgct tcgtttgcgt atacgatgtt gaatgatcgg tccataaccg ccacgcggtc
     1321 ggacagaccc tgtgctaatt gcttgatcgc ttcgattcgc gcggtaatga tgggatccgt
     1381 tggcaatggt gcgtcgggta attctgcgtc agacaagctt tctcgctctt cggcatggaa
     1441 cgcctatgac attat
//
LOCUS       DASTWE010000713         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1247453, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000713 DASTWE010000000
VERSION     DASTWE010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247453"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFT30_07390"
     CDS             <1..518
                     /locus_tag="VFT30_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092491.1"
                     /translation="LTEKSFTEHLGWPDAKVTSYVSNLLVDFTHTDQLYKVRNRQNQS
                     IHTVVELLFESEVMLEAQSLDREQDVHRHIGDFTLFMAGLFPEYLRRLKSAGLIYHKD
                     FLVDYVKTGKRSYGIVAQIGRDDAEANLPLFQKLSENFELCVTALGFVRSDLDRMQNP
                     AYQKTRDLLLN"
     gene            515..1399
                     /locus_tag="VFT30_07395"
     CDS             515..1399
                     /locus_tag="VFT30_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HEU5092492.1"
                     /translation="MKSIRPIILVHGGAGSRAMTSPQAACLRAALQVGYHLLDRGGSA
                     LIAVEHTIRVLERSGLFNAGKGSRLQLDGVQRMDASIMEGHHLRAGAVASIEGIIHPI
                     TAARLVMEETDHVLLAGPLARKFAEYFKMEPHRLRTPTRRLAYGVMLKRKKSAGDRHG
                     TVGAVALDRTGTVAAGASTGGIDRMLPGRVGDTPIIGCGVYADNDTGAVSMTGWGESI
                     IRLAVAKEICDRLAQGTAPATAARLVLQKLVSRIDGSAGCLVLTPQGQFTIRHSTPHM
                     MAGYWAGRGTPVVKDSFR"
     gene            1413..1610
                     /locus_tag="VFT30_07400"
     CDS             1413..1610
                     /locus_tag="VFT30_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEU5092493.1"
                     /translation="MVRRKGRKPKRYSQVARISVMLRRLLGGATVGELADELQVTKRQ
                     IHRDLREIEDSGYPLQQEDGR"
BASE COUNT          359 a          458 c          496 g          371 t
ORIGIN      
        1 cgttaaccga aaagagcttc acggaacacc tcggttggcc tgacgccaag gttacatctt
       61 acgtctccaa tctgttggtc gattttacac acaccgatca gctttataag gtcagaaatc
      121 ggcaaaacca atcgatccat acggtcgtgg agttgctgtt cgagtcggag gtcatgctcg
      181 aagcccagtc gctggatcgt gaacaggacg tccatcgtca cattggggat ttcactctgt
      241 tcatggccgg actctttccc gagtaccttc gacggctcaa atcggccggg cttatttatc
      301 acaaggattt tcttgtggac tatgtgaaga cgggcaaacg atcgtatggg atcgtcgcgc
      361 aaatcggccg cgatgatgct gaagccaatt tgcccttgtt tcaaaaattg tccgagaact
      421 ttgaactctg cgtgacggct ctggggtttg tgcggtccga tctggatcgc atgcagaatc
      481 ccgcctacca aaagacccgg gatctgctct tgaattgaaa tccatccgtc ccatcattct
      541 cgtgcacggg ggcgccggct cccgcgcaat gacctcccca caagccgcct gcctacgtgc
      601 cgccttgcaa gtcggctatc atttgctgga tcgcggcggc tcggcattga ttgccgtgga
      661 gcacaccatt cgtgttctcg agcgcagcgg cctcttcaat gcgggaaaag gatcgcgctt
      721 acagctagac ggagtgcagc ggatggatgc atccatcatg gaagggcatc atctgcgcgc
      781 cggggcggtg gcttccatcg aaggcatcat tcatcccatt accgcggcgc gccttgtgat
      841 ggaagaaacg gatcatgtgt tgctcgcggg acctttggcg aggaagtttg cagagtattt
      901 caagatggag ccccaccgac ttcggactcc aactcgccgt cttgcctatg gtgtgatgct
      961 taagcggaag aaatctgcag gagatcggca tgggacggtc ggagccgttg cattggatcg
     1021 aacggggacc gtggccgccg gcgcttcgac cggtgggatc gaccgcatgt tgcccggacg
     1081 agtcggggac acgccgatca tcggctgtgg cgtgtacgcg gataatgaca ccggtgccgt
     1141 gtcgatgacg ggatggggcg agagcatcat tcgtttggcg gtggccaaag aaatttgtga
     1201 tcgcttagct caaggaaccg caccggcaac tgcagcaaga ctcgttctgc aaaagttagt
     1261 ctctcggatc gacggctctg cgggatgcct ggtcctcact cctcaaggcc aattcactat
     1321 tcgccattcc acccctcaca tgatggcagg ctactgggcc ggacgcggaa cccccgtggt
     1381 caaagattcc ttccgataac agaggacttg ctgtggtccg tcgcaaagga cggaaaccca
     1441 aacgctatag tcaggtcgct cgtatcagtg tcatgcttcg ccggctcctg ggcggagcga
     1501 cagtcggaga attggccgat gagctgcaag ttacgaagcg ccagattcac cgagatctcc
     1561 gagagatcga ggattcaggc tacccgttgc aacaggaaga tgggaggtga cgacttcctc
     1621 cgggcttcaa gaggttggag gtcgcggtgg caccagtgac cgcatactca tgggatgatc
     1681 tggg
//
LOCUS       DASTWE010000714         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_536564, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000714 DASTWE010000000
VERSION     DASTWE010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536564"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFT30_07405"
     CDS             complement(<1..510)
                     /locus_tag="VFT30_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEU5092494.1"
                     /translation="MLATRITQKEGAFYFIAYKAGDLLDKVRFTSRYYFEGEEIAQAK
                     ISDHDDVAQFIAGIERSEKGFQRVLNRQKIKQIVNFYETVVAQPMIPGTVLLFTDETL
                     RFQKMDGSNSIGHLSEPKGKYLVIDGQHRLAGLHFFHDKHPDQSAQVEVPCLLFDGRS
                     ADFATEMFVI"
BASE COUNT          271 a          325 c          299 g          267 t
ORIGIN      
        1 gatcacaaac atctccgtcg caaagtcggc gctcctgccg tcaaacaaga gacatggcac
       61 ctcaacctgc gcactttgat ccggatgttt gtcatggaaa aagtgaagcc cggcgagtcg
      121 atgttgtccg tcgatcacta gatattttcc cttcggctcg ctgagatgac cgatcgaatt
      181 cgagccatcc attttttgga agcgaagtgt ttcgtcggta aaaagcagga cggtgccggg
      241 aatcatcggt tgagccacca cggtttcata aaaattgacg atctgcttga tcttctgccg
      301 attcaagacc cgttggaaac ctttctcgct ccgctcgata cccgcgataa actgcgccac
      361 gtcgtcatgg tctgaaatct tggcttgtgc gatctcttca ccctcgaagt agtaccggct
      421 agtgaatcga accttgtcca agagatcacc ggccttgtac gcgatgaaat agaatgcgcc
      481 ctctttttgc gtaatgcgcg tcgcgagcat ggagcctcca ttggtttgaa tagaagccag
      541 cgggagaatt ctacgcggct ctctgcatgg ccgcaagaca aggactgtgc taccatcgat
      601 gaccatgacg attccacgcg tggcatttgg ccttttcgtg ttgctgctct tgatactttt
      661 ggttccctcc ctcgacgcca aggcacagga gcaggagata gaggattccg gagccacaac
      721 agagttgtcg tgcagctttt caacggaaaa aggtgaaacc gggcgcccct gctacgttcc
      781 gttcccacaa ggatgccggg tcgcctccat tccgggaacc aagcagccct ggaccacgat
      841 ctcgaaaggc ggccgactgc agtgtcgatt tgatgaaaag aggaccgatt ggaagacaaa
      901 aattgtcggc acttgcgacc gctgccgatc gatccgatgt tcggcccatt tcagtgtccg
      961 ctttgattgc tcggcccagc actaagtaca cttgtatccc atgaccatca cggcagcaat
     1021 caaacaagaa gcccgcacgc tgggattcga tgcggttggg ataacgcacg tgtccagcga
     1081 acatacaggg tccgttactc gggatcagga gaacagaccc cgttcccatt ccgagactgc
     1141 ggtacctcaa cgtctcttcg ac
//
LOCUS       DASTWE010000715         1308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_439602, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000715 DASTWE010000000
VERSION     DASTWE010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439602"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFT30_07410"
     CDS             <1..288
                     /locus_tag="VFT30_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092495.1"
                     /translation="RDEQPSIWDWLVKGRFGVVKSRAEASVTPETDQSSHLRLQISSE
                     GKQTMFQGWGPTAPQVPQSAENKLRLIKNELKIVQNTYTTETFYGSYGLKP"
     gene            complement(285..>1308)
                     /locus_tag="VFT30_07415"
     CDS             complement(285..>1308)
                     /locus_tag="VFT30_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5092496.1"
                     /translation="FREELKSKGEWSALVGSSTAMLEVYKLVARVAESKSTVLLQGES
                     GTGKELIARAIHTNGPRRDKPFIPVNCGALPDTLLESEMFGYEKGAFTGAAGTKVGLF
                     ESANGGTLFLDEIGELGQALQVKLLRVMQDQEVRRVGSTTSTKVDVRIIAATNRDLEQ
                     LVKEGKFRDDLFYRLKVVPITLPSLVDRREDIPMLVHHFLQKCAAGTDHAVRGVLPET
                     MALLIQYRWPGNVRELENAIERAVSLSHGPLLTPEDLPEVIRQSATTEADARLSQVDQ
                     LDEVCLTLEEVEKRHLTRVLKETKGNKVKAAKILGIDRRTLYRMAERFGLDLGEEAEG
                     AEKEPV"
BASE COUNT          291 a          395 c          308 g          314 t
ORIGIN      
        1 agggatgaac agcccagtat ctgggattgg ctcgtgaaag gacggttcgg tgtggtcaag
       61 agccgtgctg aagcgtccgt gacgccagaa actgaccaga gctcccatct ccgtctgcaa
      121 atatcgtcgg aaggtaaaca aacaatgttc caagggtggg gccccaccgc acctcaagtt
      181 cctcaaagtg ccgaaaacaa actacgcttg atcaagaacg agctgaaaat cgttcagaac
      241 acgtacacaa cagagacctt ttacggatct tacggattga agccttagac aggctctttt
      301 tccgctccct cagcctcctc tccaagatcc aggccgaaac gctctgccat acggtagagc
      361 gttcgtctgt cgatgccgag gattttagcg gccttcactt tatttccctt tgtttccttg
      421 aggacgcgcg tcaaatgacg tttttccact tcttctaggg tcagacacac ctcatcgagc
      481 tgatcgactt gggaaagccg ggcatcggct tcagtcgttg cactctgtcg gatcacttca
      541 ggtaaatcct caggcgtcag gagaggtccg tggcttaatg acacggcacg ttcaatcgca
      601 ttttcaagct ctcgaacgtt gcctggccat cgatattgga tcaacaacgc catcgtttcc
      661 ggcaggactc cccgtacagc atggtctgtg cctgccgcgc acttttgtaa gaagtgatgc
      721 accagcatcg gaatgtcttc tcgtcgatcg accaacgacg gcaacgtgat cggaacgact
      781 ttcaaccggt agaagaggtc gtccctaaac tttccttcct tcaccagctg ttcaagatcc
      841 cggttggtcg cggcaatgat ccggacatcg actttagtgg acgtcgtgct cccaacccgg
      901 cggacttcct gatcttgcat gactcgcaac aacttcactt gtaatgcctg tccaagctca
      961 ccgatctcgt cgagaaacag cgtccctcca ttagctgatt caaagagtcc caccttggtg
     1021 cccgctgcgc cggtgaaggc tcctttctca tatccgaaca tttccgattc cagcaaggta
     1081 tctggcaacg caccgcaatt gactgggatg aacggcttat cccgccgggg accattcgta
     1141 tgaatggccc tcgcaatgag ctctttgccg gtcccgcttt ccccttggag cagcaccgtg
     1201 cttttgcttt ccgccacgcg ggcgaccaac ttgtacacct ccaacatcgc cgtgctactt
     1261 ccgaccaatg cagaccactc gcccttactc ttcaactctt ctcggaac
//
LOCUS       DASTWE010000716         1367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_795012, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000716 DASTWE010000000
VERSION     DASTWE010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795012"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="nuoB"
                     /locus_tag="VFT30_07420"
     CDS             complement(<1..518)
                     /gene="nuoB"
                     /locus_tag="VFT30_07420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEU5092497.1"
                     /translation="MDNERLSLPQYDIPQDIQNAVTVTTLDRLYNWGRRSSVWPLMFG
                     LACCAIEMIVAQTARYDMARFGMEVMRPTPRQADLMIVSGTVTKKMVPAVIRLYNQMP
                     EPKYVVAMGACASSGGPFKEGYNVVAGIDKFLPVDVYIPGCPPTPQALIAGMIKLQEK
                     IDKESLKKVRWYR"
     gene            complement(508..717)
                     /gene="iscX"
                     /locus_tag="VFT30_07425"
     CDS             complement(508..717)
                     /gene="iscX"
                     /locus_tag="VFT30_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEU5092498.1"
                     /translation="MTLNWESTYAIALELKRQHRNVNLEEVSLQQIYQWTLQLSEFED
                     DPSLANDDILYAIYQDWFEENIHGQ"
     gene            complement(714..1034)
                     /locus_tag="VFT30_07430"
     CDS             complement(714..1034)
                     /locus_tag="VFT30_07430"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HEU5092499.1"
                     /translation="MTKKNIFLILAILGFLAPYFFFVQVPPASALDLPALIQPFFANN
                     LMKGVAMDLTVSVIAFWIFVFVEASRLQMKNPWAYLLVTLLVGLSFALPLFLYFRERK
                     LETR"
     gene            complement(1035..>1367)
                     /locus_tag="VFT30_07435"
     CDS             complement(1035..>1367)
                     /locus_tag="VFT30_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5092500.1"
                     /translation="VLSIIEVASLDEAIEVNNRSQYGLSTSIFTQDVNRAFTAMRDIF
                     SGLVYINHGTTGAEIQFPFGGVRGTGNGHREAGQAALEVFTEWKSIYVDYSGKLQRAQ
                     IDNREDEM"
BASE COUNT          314 a          365 c          375 g          313 t
ORIGIN      
        1 cgataccagc gcaccttctt gagggactcc ttgtcgatct tttcctgcag tttgatcatg
       61 cccgcgatca gtgcctgcgg agtgggaggg cagccgggaa tgtatacgtc gacaggcagg
      121 aatttgtcga tgcctgcgac gacgttgtat ccttctttga aggggccacc gctggaggca
      181 caggcgccca ttgccacaac gtacttgggc tccggcatct gattgtacaa acggatcacg
      241 gccgggacca tcttcttcgt gacggtgcca gagacgatca tgagatccgc ctggcgcggg
      301 gtagggcgca tcacttccat gccgaagcgc gccatgtcat agcgcgcggt ctgcgccacg
      361 atcatttcaa tagcgcagca ggcgagaccg aacatgagcg gccacaccga ggaacgccgg
      421 ccccagttat agaggcggtc gagcgtcgta accgttacgg cattctggat atcctgcgga
      481 atgtcgtatt ggggcaaact gagtctttca ttgtccatgg atgttctcct cgaaccagtc
      541 ttgatagatt gcgtaaagga tgtcgtcgtt cgcaagcgac gggtcatcct caaattccga
      601 tagttgtaga gtccactggt agatctgttg caggcttacc tcttccaggt tcacgttcct
      661 gtgttgacgc tttagctcca gcgcaatggc gtaggtgctt tcccagttca aagtcatctg
      721 gtttccagtt ttcgctcacg gaaatagagg aagagcggca gcgcaaaaga cagacctacc
      781 aataatgtta ccaacagata agcccatggg ttcttcattt gtaatcggct ggcttccaca
      841 aacacaaata tccagaacgc aatgacggaa accgtcaggt ccatcgccac acccttcatc
      901 aagttattgg caaagaaagg ctgtataagc gcaggaaggt ccaacgcact tgcagggggc
      961 acctgaacga agaaaaaata gggagccagg aaaccaagaa ttgccaggat gagaaagatg
     1021 tttttcttcg tcatttacat ttcgtcttcg cggttgtcga tctgcgcacg ctggagcttg
     1081 cctgagtaat ccacgtagat cgatttccat tcggtgaata cctcgagagc tgcctgaccc
     1141 gcttcgcggt gcccgttgcc tgtgccgcgc acgcccccga aggggaactg gatctctgcg
     1201 ccggtcgtgc cgtggttgat gtagacaagc ccgctaaaga tatcgcgcat cgccgtgaag
     1261 gcgcggttga catcctgcgt aaagatcgat gtcgacaatc catactgcga gcgattgttg
     1321 acttcgatgg cttcatccag tgaggccacc tcgataatcg agagcac
//
LOCUS       DASTWE010000717         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1570576, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000717 DASTWE010000000
VERSION     DASTWE010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570576"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..828
                     /locus_tag="VFT30_07440"
     CDS             40..828
                     /locus_tag="VFT30_07440"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU5092501.1"
                     /translation="MTAMTGAMHSCKVEEGVALVTLNHPPVNALTPELLTELNTTFDS
                     LAMDDAVKAVVLTGTGRFFVAGADIRVLASIPSSVEGEAMARQGQMILNKIEAFEKPI
                     IAAINGSCLGGGLELAMCCHIRLAAEGTRLGQPEINLGIMPGFGGTQRLPRLIGQSKA
                     MELILTGEPVSAREALSLGLVSQVVSADDLLRQALGLARTMAAKSQVALRTSLRAIRR
                     SGELTLFDGLALEARLFGELCDTEDRKEGTAAFLEKRQPRFKNR"
     gene            854..>1668
                     /locus_tag="VFT30_07445"
     CDS             854..>1668
                     /locus_tag="VFT30_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5092502.1"
                     /translation="MQAHRSTKLALALTGGGFTGYLFEVGALTGLDDLLGDSFTSNDF
                     DMYVGVSAGSAVAALLAQGVTPQEILETNLSGKRPYYFEHRDIFRPAIGEGVKTIWRA
                     TKQLIPLVRLYARNYQEMTFIDLLDKAQDALPSGIYRLEPFAKYLEKTFMARGLGLRF
                     ADLRKDLYIPAIDLESGECVIFGEEGRRDISIAQAVTASSAAPIYFCPVRIDGRDYID
                     AGIGRPSFFDLAIAKHVDLMVMINPMGRIPLPGSSWCGSAHAKPLSRLRDKGF"
BASE COUNT          359 a          466 c          480 g          363 t
ORIGIN      
        1 cggatcagcc tccgaatagg gcaaggaagg aaatgcatca tgaccgccat gacgggagcc
       61 atgcacagtt gcaaggttga agagggcgtt gccctcgtaa cgctcaatca tccgcccgtc
      121 aacgccctca cacctgagct actcacagag ttgaacacca cattcgattc acttgcaatg
      181 gatgacgctg tgaaggccgt cgtcctcacc ggcaccggcc gattttttgt cgctggtgct
      241 gacatccgag tactggcctc tattccatcg tccgttgagg gcgaagcgat ggcccgccag
      301 gggcagatga ttttgaacaa gattgaggct tttgaaaaac caatcattgc ggcgatcaac
      361 ggttcgtgtt tgggaggagg cttggaattg gccatgtgct gtcacatccg tcttgcagca
      421 gaaggcacca gacttggcca gccggaaatc aacctgggca tcatgccggg attcggcggc
      481 actcaacgcc ttcctcggct gatcggccaa tccaaggcca tggaattgat cctgacaggc
      541 gagcccgttt cggctcggga agccttgagt ctgggtttgg tgtcccaggt ggtatcggct
      601 gatgacctgc tgcgccaagc gctgggactg gctcgcacga tggctgcgaa aagccaagtg
      661 gcgctccgga cgtcgcttcg agcaatccgt cggagcgggg agctgaccct ttttgacggc
      721 ctagccttgg aggcccgtct gttcggcgaa ttatgtgata ccgaagatcg gaaagaagga
      781 acagccgctt tcttggagaa acggcaacct cggttcaaga accgttaggc ccctcaggag
      841 acaggaagac gtcatgcagg ctcatcgttc cacaaaatta gctctggcac tgacgggggg
      901 aggtttcacg ggttatctct tcgaagtcgg cgccttgacc gggctggacg atctcctcgg
      961 tgactcgttc accagcaacg atttcgacat gtatgtcggc gtgagcgccg gatcagccgt
     1021 cgccgcccta ttggcacaag gggtcacgcc ccaagagatc cttgaaacga atctgtccgg
     1081 gaaacgtccc tattacttcg agcatcggga tattttcaga cctgcaatcg gcgaaggtgt
     1141 aaagacaatc tggcgcgcaa cgaaacaact gatcccgttg gtgcgactgt atgcgcgtaa
     1201 ctatcaggag atgacgttta tcgacctgct ggataaggct caggatgcac tccccagtgg
     1261 catctatcga ttggaaccat tcgccaaata cctggaaaag acgttcatgg ccaggggctt
     1321 gggactgcgg tttgccgacc ttcgaaaaga tctgtacatc ccggccatcg atttggaatc
     1381 cggggaatgc gtgatcttcg gtgaggaagg ccggcgggac atttccatcg cccaggccgt
     1441 gactgcctcc tccgccgcgc cgatctattt ctgtccagtg cgcatcgatg gccgcgatta
     1501 tatcgacgcg ggaatcgggc ggccatcgtt cttcgacctg gccatcgcga agcacgtaga
     1561 cctcatggtg atgatcaacc caatgggtcg cattccactc cccggttcat catggtgcgg
     1621 ttcagcccac gccaaacctc tgtctcgcct tcgtgacaag ggattttt
//
LOCUS       DASTWE010000718         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1118569, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000718 DASTWE010000000
VERSION     DASTWE010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118569"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..574)
                     /locus_tag="VFT30_07450"
     CDS             complement(2..574)
                     /locus_tag="VFT30_07450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5092503.1"
                     /translation="MAGDEFLRLRNELLASAHVEVLELGIGTGLNLPHYPDTVTALHA
                     VDPAQLLPKTIAARSTRLSFPVRIQKGTAETLTHANRRFDYVVSTWTLCTIPDPVLAL
                     QEVGRVLKPGGMFLFMEHGRSDDRKIAAWQDRLNPIQNVIGCGCNLNRQIDRLITQGG
                     LKITHLDRFTMKAVPRLGGEMYRGTAIGVG"
     gene            746..>1512
                     /locus_tag="VFT30_07455"
     CDS             746..>1512
                     /locus_tag="VFT30_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5092504.1"
                     /translation="MSKGYDHRAIETKWQQYWDEHRTFRVTEDPGKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMRGFNVLHPMGWDAFGLPAEQYAVKTGIHPAMTT
                     AENIATFKRQMKRVGLSYDWERELSTTDPQYYRWTQWIFLKLFERGLAYVAEVPVNWC
                     PALGTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYADRLLEDLKLVDWPPSTLE
                     MQKNWIGRSIGADVEFSLADQKGVIRVFTTRPDTLFGA"
BASE COUNT          348 a          392 c          428 g          344 t
ORIGIN      
        1 tttaaccaac tccgatcgct gtgccccggt acatctcacc gcccagtctg ggcacagcct
       61 tcatggtgaa tcgatccaga tgtgtgattt tcaggccgcc ttgagtgatt aaccgatcaa
      121 tttgacgatt gagattacat ccgcatccaa tcacgttctg aatcggattc agccggtctt
      181 gccatgcagc gatctttcga tcatcgctcc ggccatgttc catgaataga aacattcctc
      241 ccggtttgag gacacgtccg acttcttgca gcgcgagcac cggatcggga attgtgcaga
      301 gcgtccaggt gctgacgaca taatcgaaac gtcgattggc atgggtgagg gtttcggcgg
      361 tgcccttttg aatgcgaaca ggaaacgaga ggcgggtgct gcgtgctgcg attgttttcg
      421 ggaggagttg ggctggatcg accgcatgca gcgctgtaac ggtgtccgga tagtgcggca
      481 gattcagacc agttcctatc cccagttcta ggacttcaac atgggctgaa gcgagtagct
      541 cattgcgcaa gcggagaaac tcatcaccgg ccatgaccca ctccatgagg cgagggaaga
      601 tgtgatcgca gtagaatccc attgctgggg tagtataaca aaggttcacc cttcggcttc
      661 ggaacttgtg accccaccgg ggctatgtta gattccccag ttcaattggc gttcaacctt
      721 tggtcgtcaa cacgcaacta tagttatgag caagggatac gatcaccgcg cgatcgagac
      781 gaaatggcag cagtactggg atgagcaccg gacgttccgc gtcacggaag atcctggaaa
      841 gccaaaattt tattgcctcg acatgtttcc ttacccttcc ggttctggac tgcatgtcgg
      901 ccatcttgag ggttacaccg ccacagacat tgtctctcgc tataaacgca tgcgtgggtt
      961 caacgtgctg cacccgatgg gctgggacgc gtttggtctg ccggccgaac agtacgcggt
     1021 gaaaacaggc attcatcccg ctatgacaac agccgaaaac atcgccacct ttaagcggca
     1081 gatgaaacga gtcggactct cgtatgattg ggagcgagag ctcagtacca cggacccgca
     1141 gtactatcgc tggacccaat ggattttcct gaagctcttc gaacgaggat tggcttacgt
     1201 cgcagaggtg cctgtgaatt ggtgcccagc cctcggtaca gtgctggcga atgaagaaat
     1261 cgtcgatggc aagagtgaag tcggcggttt cgacgtgatt cgcaagccga tgcggcaatg
     1321 ggtgctgaag atcaccgcct atgccgatcg attgctggag gatttgaaac tcgtcgactg
     1381 gcctcccagt acgctggaaa tgcaaaaaaa ctggatcggg cgatcgatcg gcgcggacgt
     1441 tgagttttca ctggccgatc agaagggcgt gattcgagta ttcacaacca gaccggacac
     1501 gctgttcggt gc
//
LOCUS       DASTWE010000719         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_892336, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000719 DASTWE010000000
VERSION     DASTWE010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892336"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..346
                     /locus_tag="VFT30_07460"
     CDS             131..346
                     /locus_tag="VFT30_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU5092505.1"
                     /translation="MSRQAHSENGIIHVPSKYSVPETIRQLDGVVKSKRLTVFAHIEF
                     SGDAAKVGLHMRPTQLLIFGNPNPVRR"
     gene            749..>1580
                     /locus_tag="VFT30_07465"
     CDS             749..>1580
                     /locus_tag="VFT30_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU5092506.1"
                     /translation="MQAVGYKKPLPIGEAESLMDIEVPTPTAGGRDLLVKVEAISVNP
                     VDVKVRTRSAPEGMLYKILGWDAAGVVVAVGPESNLFKVGDEVYYAGSLTRPGTNAEF
                     HVVDERIVGRKPKSLDFTAAAALPLTTITAWELLFDRFGVRLGKPSDAGSLLIVGAAG
                     GVGSILIQLARRLTGLTIIATASRQETRRWCLELGAHYVIDHTKPLVAQLRDIAIPQV
                     EFIAGLNATDKHYPVLVDALAPQGKFGLIDDPGALDITLLKRKSASLHWEFMFARPVF
                     Q"
BASE COUNT          341 a          400 c          490 g          349 t
ORIGIN      
        1 catcgcagaa gacgcagtga tcgatgcgca gatgctgccg aaggtccaga tgccgaatcg
       61 gaacggcttc attccggatc cacggcccga tgtgcggtag ccggtaaatg ctgtgcacgg
      121 gagtggtacg atgtcacgtc aggcgcattc cgaaaacggc atcattcatg tgccgagcaa
      181 gtactcggtg cctgaaacca ttcgacaact ggacggtgtt gtgaaatcga agaggctcac
      241 ggtgtttgcg cacatcgagt ttagcggtga cgctgccaag gtcggtcttc atatgcggcc
      301 gacgcagttg ctgatctttg gcaatcccaa tccggtacgc cgttgatggt cggctcggcc
      361 agcatcgcac tcgatctgcc gttagagact ctggcgtgat acgaccaacg tcgaattggc
      421 catcaagcta gccggttcct atcaccagag ccaggtagta gaaagttgca cggttcggga
      481 gaagttgatg tattgcagcg caaagtgtgc attggagcat cccggcaatg ccaaatgtga
      541 cggcggtatc ttggctgcaa aggacaccga ccttttctca atcttagcgg tggggcgcat
      601 ggtttcacgt gctaatcaag tccttgccat gacttcagcg tgacagcaga tcatacaagg
      661 acaatcaggc gaggagaggg ctgttgcgat taatttcttg tgatcgtcgc ccaatccgaa
      721 atcgggatgt gaggcgaaag gaaacagcat gcaagcggtt ggatataaaa agccgttgcc
      781 gatcggcgag gctgagtcgc tcatggatat tgaggtgccg acgccgacag caggcgggcg
      841 tgatctgctg gtgaaggtgg aggccatctc cgtcaatccc gtggacgtga aggtgcgcac
      901 ccgatctgca ccggaaggga tgctttacaa aatcttaggc tgggatgcag ccggagtggt
      961 cgtcgcggtg ggacccgagt ccaatctttt caaggtagga gacgaggtct attatgcagg
     1021 atcgctcacg cgtccgggaa cgaatgcgga atttcatgtg gtggacgagc ggatcgtcgg
     1081 aaggaagccg aagagcctcg atttcaccgc tgctgcagct ctgccgctca ccacaatcac
     1141 ggcctgggaa ctgctcttcg atcgcttcgg cgttcgtctg ggcaagcctt ccgatgcggg
     1201 tagtttgctg atcgtcgggg cggctggtgg cgtcggctcg atcctgatcc agttggcacg
     1261 tcggctgaca ggactcacga tcatcgcgac ggcgtcgcgt caggaaaccc gccggtggtg
     1321 cctggagctt ggcgcacatt atgttatcga tcacacgaag ccattggttg cgcaattgcg
     1381 cgacatcgcg attccgcaag ttgaatttat tgccggtctc aacgcgacgg ataagcacta
     1441 tccggtattg gtagacgcac tggctccaca agggaagttt ggcctcattg acgatccggg
     1501 cgcactcgat atcacgttgc tgaaacggaa gagtgcttcg ctgcattggg agttcatgtt
     1561 tgcgcggccg gtttttcaga
//
LOCUS       DASTWE010000720         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_439772, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000720 DASTWE010000000
VERSION     DASTWE010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439772"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFT30_07470"
     CDS             complement(<1..897)
                     /locus_tag="VFT30_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5092507.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQEAIARGVDIINLGIGD
                     PDLPTPIPIIESLAKAAKDPKHHQYPSYEGMLTFRKAVAGWYQRRFNVTLDPMDEVLT
                     LIGSKEGIGHIHLAFVDPGDVVLVPSPGYPVYPVGTSFSGGTSHIMPLTKANGFLPDL
                     SAIPKEVAQKAKLMWLNSPNNPTSVIMTKDYFKRVVEFAQENRVIVCHDAAYSEVYYD
                     GRRPASFMEAEGAKNVGVEFHSLSKTYNMTGWRIGFAVGNKEVLAGLGKVKSNLDSGC
                     FEAVQEAGITALGLDDSVTDNLR"
     gene            complement(1048..1731)
                     /locus_tag="VFT30_07475"
     CDS             complement(1048..1731)
                     /locus_tag="VFT30_07475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU5092508.1"
                     /translation="MRVVCLQHVPFEGPGAFAKALAKRGVGLEYSLVPKEGVPKDGGD
                     LLIVMGGPMSVNDSDKWITEETAFIRSALLAGQPMIGVCLGSQFMAKALGATVRSGQA
                     LEIGMTPVRLTDAGKQDPVFGAGPESFDVFEWHGEVFDLPKDCAPLAGSDVAPLQAFR
                     YGDRAYGLLFHLEMEKGGIDSLCQECAPDLMKARLTAHQVKSGALPQLPQLHQMADRL
                     IGHLLQSSR"
BASE COUNT          439 a          518 c          464 g          406 t
ORIGIN      
        1 tctcaagttg tcggtcacag agtcgtccag acccagggcg gtaatgccgg cctcttgcac
       61 cgcctcaaaa cagcccgagt ctaagttgct ttttaccttt ccgaggccgg ccaagacctc
      121 cttgttgccg acggcaaagc cgatacgcca gccggtcata ttgtaggtct tggaaagtga
      181 gtggaactca actccgacgt tctttgcacc ctcggcttcc ataaaactcg ccgggcgacg
      241 cccatcataa tacacctccg aataggcggc atcgtggcac acgatcaccc gattctcctg
      301 cgcaaattca accacgcgtt tgaaatagtc cttcgtcatg atcacagagg tgggattgtt
      361 cggagaattg agccacatca gcttggcctt ttgcgccacc tctttcggga tcgcgctgag
      421 gtcaggcaag aaaccgttgg ctttcgtcag cggcatgatg tgagacgtgc cgccggaaaa
      481 actggtaccc acgggataca ctggatagcc agggctcgga accaagacga catcccctgg
      541 atcgacaaag gcaagatgaa tatgtccgat accctccttc gaaccgatca gcgtgaggac
      601 ctcgtccatt ggatcgagcg tcacattgaa tcggcgttgg taccaaccag ccacggcttt
      661 cctgaaggtc aacatgcctt cataggaggg atactggtga tgcttcgggt ccttggccgc
      721 cttcgccaaa ctctcgatga tgggtatcgg cgtcggcaaa tcggggtcgc cgatgccaag
      781 attaataatg tccactccac gcgcgatggc ctcttgcttc attttgtcga tcgcggcaaa
      841 caaataggga ggcagggtct taatgcgcgt ggcaacttcg ataggaaaac cggccattat
      901 gatcttactc ctttcgtaaa tgagttcgtc actacgccgg caggtcgtca tgtcctcaag
      961 tctggcaaac ttttgtggac tttcgacgtg atgacgatgg gacttgtcga cgaagggaat
     1021 aagactactt caggaatgag ccggtaatca acgacttgat tgaagtagat ggccgattaa
     1081 ccggtcagcc atttgatgaa gctgcgggag ctgtggcaga gcgcctgatt ttacttgatg
     1141 tgccgtaagg cgagctttca ttaagtccgg cgcgcattct tgacagagtg agtcgatccc
     1201 gcccttctcc atttccaaat gaaagagaag gccataggct cgatctccgt agcgaaaggc
     1261 ttgcagtggc gcaacatcag aaccagccaa cggcgcgcag tccttcggca agtcaaagac
     1321 ctcgccatgc cattcaaaga catcgaaaga ttcagggcca gccccaaaca caggatcttg
     1381 tttccctgca tcagtgaggc gaacaggagt catgccgatt tccaatgcct gacctgaccg
     1441 cacggtggca ccgagcgcct ttgccataaa ttgacttccc aaacacactc cgatcatcgg
     1501 ctgaccggcg agcagtgcag acctgatgaa ggcggtctcc tcggtaatcc acttgtcgga
     1561 atcgttcact gacatcggtc cgcccatcac gatcaacaag tcccctccgt ctttgggtac
     1621 accttctttc gggacaagtg aatattcgag acccacacca cgcttagcca gagcctttgc
     1681 aaaagcaccc ggaccctcga agggaacatg ctggagacag acgacgcgca ttgatcccct
     1741 ctcttagaga gacttcgcct gttatatacc gaatggcgca atgaataaaa agcgtttgcc
     1801 acgcacaatc atctggtcac cctctcc
//
LOCUS       DASTWE010000721         1412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_859783, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000721 DASTWE010000000
VERSION     DASTWE010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859783"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFT30_07480"
     CDS             <1..204
                     /locus_tag="VFT30_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092509.1"
                     /translation="HTIVKTFFLTLVIFGPLFLRQRLTRHTILTLNPTAKVNKLTAFR
                     TEGTERIVFPLDWLTAGWTLHES"
     gene            complement(229..945)
                     /locus_tag="VFT30_07485"
     CDS             complement(229..945)
                     /locus_tag="VFT30_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5092510.1"
                     /translation="MNALMEQKRARDLTPEDRAEIIRRLEFGLTLDDALVNDMARNSV
                     VLDFRRRRFVYRAGEPADCLYAIIHGRIKLCRIESDTGREAVIDILPEGSLFGESALY
                     SIAGQRANSAIAYENSRLLRIQVGDFKRGMAEDDRLHDYTFRLIGQRLDHAERRLADF
                     ALNAIPARLDSLLADFSMKYGVSEPEGVLIDIPLPHREIASIVGSTRESVTVRLNAMR
                     REGTIAFVKRRILLKRPASL"
     gene            1211..>1412
                     /locus_tag="VFT30_07490"
     CDS             1211..>1412
                     /locus_tag="VFT30_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5092511.1"
                     /translation="MESKSNILVVDDNPDKLNLIKAALELAGYNVTTATDGDEALAAI
                     ESYQPDLVITDVMMPRMNGYELA"
BASE COUNT          350 a          365 c          384 g          313 t
ORIGIN      
        1 catacgattg tgaaaacgtt ctttttaact ctcgtcatct tcggtcccct gttcctgcgg
       61 cagcgcctca ccaggcacac gatactcacc ctcaacccaa cggccaaggt caacaagttg
      121 acagcgttca gaacagaagg gacggaaagg attgtctttc cactcgactg gcttaccgca
      181 ggttggacac ttcatgaatc gtaacgccac gaaacggcgc gcatcgcatc aaagcgaagc
      241 gggacgtttg agcaatattc gtcgtttgac gaaagcgatc gtaccttcgc ggcgcatggc
      301 attcaagcgg accgtgacac tttcacgagt tgatccaaca atggaggcta tttcgcggtg
      361 gggcagaggg atatcgatga gcacgccttc gggttcggaa accccgtatt tcatggaaaa
      421 atcagcaagt aaactgtcaa gacgggcggg aatggcattg agcgcaaaat cagcaagccg
      481 gcgctccgcg tgatcaagtc gttgaccgat caggcggaaa gtgtaatcat gaaggcggtc
      541 atcctcagcc atcccccgtt tgaaatcccc gacctggatt cgcaggagcc tggaattttc
      601 gtaggcgatt gcagagttcg cccgttgccc cgcaatagag tagagagctg attccccaaa
      661 taaggatccc tcgggaagga tatcaatcac ggcttcgcgc cctgtgtctg attcaatacg
      721 acaaagtttg atacgaccat ggataatcgc gtagaggcag tcggcaggtt cccccgcacg
      781 atagacgaag cgacggcgtc ggaagtcaag gaccactgaa ttacgagcca tgtcgttaac
      841 gagggcatcg tccagtgtca ggccaaattc taaccggcgg attatttcag cgcgatcttc
      901 gggagttaaa tcccgggcgc gcttttgttc catcagggca ttcatctcca tgtccttcgg
      961 ggcatcgagt tacagtttcc agcttgaatc agttgacggt atttgctctt gttcaacgtg
     1021 gtcctgtgcg ggttaatttc tatgacgact gtgaaggaca cccgcgcgcg gttcccagga
     1081 ttgttactct cgattgaatc ttttgacgaa ttgctagggc gagtacagac acgttgaacc
     1141 gaggctcgaa ctgtagcatg aaggctcggg ggcgtgcaag aacaataagg aagcaagaac
     1201 catctcgttt atggagtcaa aatccaacat tctcgtagtg gatgataatc ccgataaact
     1261 gaacctgatc aaggcggctc tggagcttgc aggttataac gtaaccacgg cgacggacgg
     1321 cgatgaggcg ctcgcggcca tcgagtcgta tcagccggac ctcgtgataa ccgacgtcat
     1381 gatgccccgc atgaacggtt acgagctggc cg
//
LOCUS       DASTWE010000722         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_148919, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000722 DASTWE010000000
VERSION     DASTWE010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148919"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(630..>1035)
                     /locus_tag="VFT30_07495"
     CDS             complement(630..>1035)
                     /locus_tag="VFT30_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092512.1"
                     /translation="ARVVSACPAIEIIHSRFIDFNLVTHCERIADHFVNGAFVRGAPR
                     RDLGGLDLSKAHVTLTVDGIVVIDAIGGHPANDPILPVVDLANHFRNERGLKAGETVT
                     TGSFTGLVRVTSGQTVRATFDGLGSVQARFTR"
BASE COUNT          245 a          282 c          280 g          228 t
ORIGIN      
        1 acggccattt cccagtggta gttcgccaaa gtatgcttag ccgccgttcg atctactttg
       61 atcagtcgtc gcacggtaga atctttgtca tgaaacgaat ggcaggcgcc gcgaggataa
      121 ctacgggcag catcatcatc caggaaagta accacgcccg catccaatac tccatgaaaa
      181 tgccggatgc tatgaacggg tagctcgcga tcgccgcagc gatcgcacag gtcaaacccg
      241 attgtatgat tccgaaaaca agatgactgt ggcggcgtgg gataagcatg caggaggcct
      301 cgtgcttccc tccctgccaa gcaaatgccg tgccgctgct taggacgtcc gcgcattggc
      361 cgcgggcatg tacctgtcgg cttccggcac tagcggctct tccctggaag aatgtctgct
      421 gcccggttta atgcacacgg cgacgctcat ctcagatgtt ccgtaatcga tcttatgcga
      481 acacccgttg atcttagtga agagggcgtt caccagcaac tctctcggtg gaaagggaca
      541 gctctctaac tcgctctaat acgcgagcgt agccctgcgt acgattgagt gtaccaaacc
      601 tcatccgcat tgttgttgtt atggaatggt cacctcgtga accgagcttg tacgctgccg
      661 agaccgtcga aggttgctct tacggtctga ccggaagtaa cacggacaag gccagtgaac
      721 gatccggtgg ttaccgtctc gccggctttc aatcctcgtt cattcctgaa atggtttgca
      781 agatcgacaa ccggaagaat tgggtcgttt gcgggatgac caccaattgc atcgatcacg
      841 acgatgccat cgacagtcag cgtgacgtgg gcctttgaca aatcgagtcc acccaaatcg
      901 cgtcgaggcg ctcctcgtac gaatgcgcca ttgacaaaat gatcggcgat acgctcgcaa
      961 tgtgtgacca aattaaagtc gataaatcgc gaatggatga tttcgatggc gggacaggcg
     1021 gagacaacgc gagcg
//
LOCUS       DASTWE010000723         1291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1570840, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000723 DASTWE010000000
VERSION     DASTWE010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570840"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..403)
                     /locus_tag="VFT30_07500"
     tRNA            complement(328..403)
                     /locus_tag="VFT30_07500"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:Lys,seq:ttt)
     gene            complement(518..>1291)
                     /locus_tag="VFT30_07505"
     CDS             complement(518..>1291)
                     /locus_tag="VFT30_07505"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEU5092513.1"
                     /translation="QVDKVFDQYMGYADRLKTHITDTTVLLNRAIDKNKTVLFEGAQG
                     TNLDVDFGTYPYVTSSSAAAGGACTGTGVGPTMIDAVLGIAKAYSTRVGSGPFPTELT
                     DEIGRGLQERGREFGSTTGRARRCGWFDAIVVRHATVVNGLTSMALTKLDVLDGCKEL
                     KVCIGYRHGSTMYKTMPADLDVLANCEPVYQRMKGWTTATTGMTSYKRLPVEAKRYLA
                     RIEELAQCRIDMISTGSKRAETIMLRNPLDCSRRRPKRS"
BASE COUNT          289 a          370 c          361 g          271 t
ORIGIN      
        1 cgaaagcaca accaccacga ctatggcgat gtcggaaatg atccatcgat aatgaaagat
       61 gccgctgaga cgctcgtggc ctagcagcca ttgagtcggt tgatgaacat ttcatggata
      121 ccggcagcct catcaaccgc catatagagc aggataagcg agagaacacc ccaatgagaa
      181 taggggtcaa gggaagccct ttgaggacgg cgatgaggac gagcaagagc ggcttaggaa
      241 ggtcttcaat cgggagtaaa accgggagta ggccgcttca gaaagacaaa ggggttaccc
      301 caagtgaggc aaccccttga tttaaagtgg tgagccggct gggactcgaa cccagggccc
      361 tcgccttaaa agggcgatgc tctaccgact gagctaccgg ctcactgaaa ggatacagga
      421 cctggtcgct aaccttcttg cattcccctc aaactgtagt ggacaaacgc cggtctatcc
      481 tagcattccc gcccggccaa tgaccattga ctagttttca agagcgtttg gggcgccggc
      541 gagagcagtc caaaggattg cgcagcatga tcgtctccgc acgtttggac ccggtcgaga
      601 tcatgtcgat tcggcattgc gccagctctt cgatgcgggc caagtaccgc ttcgcttcga
      661 ccgggagtcg cttatagctg gtcatgccgg tcgtcgcggt ggtccaccct ttcatccgtt
      721 gatagacagg ctcgcagttg gccaacacgt ccagatcggc tggcatggtc ttatacatgg
      781 tgcttccgtg tcgatagcca atacagactt tcaactcctt gcagccgtcg agaacatcga
      841 gtttggtcag cgccatcgag gtgagtccat tcaccaccgt cgcgtgacga accacgatcg
      901 cgtcaaacca accgcaccgc ctcgcccgtc ctgtggtcga tccaaattct cgccctcgtt
      961 cctggagccc tcgtccgatc tcatcggtca gttcggtggg gaacggccca ctgccgactc
     1021 ttgtcgaata ggccttggcg atgcccagga ccgcgtcgat catggtggga ccgacaccgg
     1081 tgcccgtgca ggcgccgcct gccgcggcac tggacgaagt cacataggga taggtgccga
     1141 aatccacatc cagattcgtg ccctgagccc cttcgaacag gacggtcttg ttcttatcga
     1201 ttgcacgatt cagcaatacc gtggtgtcgg tgatgtgggt ctttagtcgg tcggcataac
     1261 ccatgtactg atcgaagact ttatcgacct g
//
LOCUS       DASTWE010000724         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_181383, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000724 DASTWE010000000
VERSION     DASTWE010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181383"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="thpR"
                     /locus_tag="VFT30_07510"
     CDS             complement(<1..622)
                     /gene="thpR"
                     /locus_tag="VFT30_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU5092514.1"
                     /translation="MIRTFLAVELGDELRNQITLTQQDLKQRLVRESSKDIRITWVQP
                     SSIHLTMKFLGDTDEHLIEPLREAIERAMPGHGAVHMPIERLGVFPRLLQPRVLWIGP
                     SEQWEQSDDAKRLVALHRAVENCCQSVGFAPEGRPLSPHLTLARVKEGERHVGQALAE
                     SGAMDRPVSLGLLAVESIVLMKSELRPTGSVYTKLWVVRTGGNRRDD"
     gene            complement(619..>1553)
                     /locus_tag="VFT30_07515"
     CDS             complement(619..>1553)
                     /locus_tag="VFT30_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEU5092515.1"
                     /translation="NRGQLRQALIPSRATVLPNPVGSAPGFVLTWRNAYVIALPGVPS
                     EMQAMMQESVIPFLADQLDREKSGRSRPITRLIFHTWGLPESDVNTKLQGLIQKRTPV
                     ALGLLASPTGVLVSLTTRAHRSIKEAVLLSLAEGVRTRLREWICAEGQDTLEEVVGRL
                     LQRQKLTVAVAESCTGGLIGYRLTQVPGSSAYVDRGALCYSNQAKTDMLDVPTRLIEQ
                     YGAVSREVAEAMAKGMRDRAGASVALSVTGIAGPGGATETKPVGLVYIGLDGGDAVMT
                     KEFRFHGDRSIIRQRAAQAALDMLRRWLIARGGA"
BASE COUNT          314 a          494 c          415 g          330 t
ORIGIN      
        1 catcatcgcg gcggttacca cccgttctca ctacccacaa cttcgtatac acggatcctg
       61 tcggtcgtaa ttcacttttc atcagcacga tggactccac cgccagcaag cccaatgaaa
      121 cgggccggtc catcgcaccg ctttcggcca gagcctgacc aacgtggcgc tcgccttcct
      181 tgacccgtgc aagcgtcagg tgtggactta gcggcctgcc ttccggtgcg aagccgaccg
      241 attggcaaca attctccacg gcacgatgta gtgccactaa tcgtttcgcg tcatcgctct
      301 gctcccactg ctccgatggt ccgatccaga gcacccgtgg ctggagaaga cgagggaata
      361 cgccgagccg ctcgatcggc atatggacag ccccatggcc tggcattgct cgttcgatgg
      421 cctcacgcaa gggctcgatc aggtgctcat ctgtatcacc gaggaacttc atcgtgaggt
      481 gaatggatga cggctgcacc caggtgatcc gaatatcttt tgaggattca cggacaagtc
      541 gttgcttgag gtcttgctga gtcagcgtga tctggttgcg tagttcgtct cccaattcca
      601 cggcaagaaa agtccgtatc atgccccccc tctggcgatg agccatcgac gaagcatatc
      661 aagagccgcc tgagcagcac gctgccgaat gatggaccga tcaccgtgga accgaaactc
      721 ctttgtcatg acggcatcac cgccgtcgag cccgatgtag accaacccga cgggcttggt
      781 ctctgtcgca ccgcccggcc cggcgatgcc cgttacgctc aatgccaccg acgcacccgc
      841 gcggtctcgc atccctttgg ccatggcttc agctacttct cgactgaccg ctccatattg
      901 ctcgataaga cgtgtcggca catcgagcat gtccgtcttg gcttggttgc tgtagcagag
      961 cgccccgcga tcgacatagg ctgaagatcc cggtacttgt gtgagccggt aaccaataag
     1021 acctcccgtg cacgactcag ctaccgcgac agtgagtttc tgccgttgga gcaaccgacc
     1081 gaccacttct tccaacgtgt cttgtccctc ggcacagatc cattctcgca gtctcgtgcg
     1141 aaccccttcc gccaatgaca gaagaacggc ttccttgatc gatcgatgag ccctggtcgt
     1201 aagcgacacc agcactcctg tcggcgaggc cagaagcccc aacgcaaccg gggtgcgttt
     1261 ctgtatgagt ccctgcagct tggtattcac atcggactcc ggtaatcccc aggtatgaaa
     1321 gatcaatcgt gtgataggac gggaacgtcc cgacttctct cggtcgagtt gatccgccaa
     1381 gaacggaatg accgattcct gcatcatggc ctgcatctcg ctcgggacac ctggcagtgc
     1441 gatcacgtat gcgttccgcc atgtcagcac gaagccgggt gcggacccga cagggttggg
     1501 cagtaccgtc gcccgtgacg gaatcaaggc ttgccgcaat tgtccgcgat ttg
//
LOCUS       DASTWE010000725         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_84461, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000725 DASTWE010000000
VERSION     DASTWE010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84461"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VFT30_07520"
     CDS             <1..857
                     /locus_tag="VFT30_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU5092516.1"
                     /translation="MDQETSDHPPHLYSDNRSSSYTPADKDTEFLQRDELRSVRIGLE
                     LLKPELIQREEGIRSTIVVFGSARLQEPVVLKEALRLAEEQVAQAPDDRIRLQRVAII
                     KRQLELATYYDVAREFGRLVSATCQIEGRCDYVIVTGGGPGIMEAANRGAADVKAKSI
                     GLNITLPHEQHPNPYITPRLSFQFRYFAIRKMHFLIRAKALVAFPGGFGTLDELFETL
                     TLLQTGKTKSVIVVLVGRDFWERVINWQLLVECGLITQADLNLFHYAETAQEVWNVIT
                     RQNGMPTA"
     gene            904..>1505
                     /locus_tag="VFT30_07525"
     CDS             904..>1505
                     /locus_tag="VFT30_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5092517.1"
                     /translation="MLEVPGFRLLFDCGMFQERREEAVRRNRDLGFEPKSLGAVLLSH
                     AHVDHSGALPVLPRQGFSGKVYLTRASADLTEIMLEDSARVQENDCRYVNKQERRRGK
                     TCIRPFYDGDEVRKIVRRFEGAQYGDQLKIAPRITASFHDAGHILGSAAVRVKYTARG
                     NTTTVLFSGDLGRSHMPILRDPEPPPPCDVLILESTYGDR"
BASE COUNT          334 a          426 c          425 g          320 t
ORIGIN      
        1 ccatggatca ggaaacatcc gaccatccac cccacctcta cagtgacaac cgctcctctt
       61 cctatacccc cgccgataag gataccgagt tccttcaacg agacgaactt cgctccgtcc
      121 gaatcggact tgagctgctg aagcccgaac tgattcagcg agaagaaggg atcaggtcca
      181 ccatcgtcgt cttcggaagt gccagacttc aggaaccggt ggtgctcaag gaggcgctgc
      241 gcctggcgga agagcaagta gctcaggctc cggacgatcg catccgcctg caacgggttg
      301 ccatcatcaa acggcagctt gaactggcaa cgtattatga tgtggcgcgg gaattcggtc
      361 ggttggtgtc cgcaacgtgc caaattgagg ggaggtgcga ctacgtcatt gttaccggag
      421 gggggcccgg tattatggaa gcggccaacc gcggagcggc ggacgtaaag gccaaatcaa
      481 tcgggctcaa tattactctg ccgcacgaac agcacccgaa cccctacatc acgccgaggc
      541 tcagttttca atttcgttat ttcgcaatca gaaaaatgca ttttctcatt cgcgccaagg
      601 cgcttgtcgc ttttcccgga ggattcggca cgctagatga gttgttcgaa acgctcactc
      661 tgctccagac cggcaaaacc aaaagcgtga tcgttgtgct ggtcggacgg gacttttggg
      721 agcgcgtgat caattggcaa ctgctcgttg aatgcggcct catcacgcag gcagacctca
      781 acctgtttca ttatgctgaa acggctcaag aagtctggaa cgtgatcaca cgccaaaatg
      841 gaatgcccac cgcatgaaac tctcattcta tggggccgct cgttcggtca ccgggagcgg
      901 catatgttgg aagtaccggg tttccggctg ctgttcgatt gtggaatgtt tcaggagcgc
      961 cgagaggaag cggtccggcg aaaccgcgac ttggggtttg agccgaaatc cctcggcgcc
     1021 gtcttgctct cgcacgccca tgtcgaccat tcgggcgccc ttcctgtttt gccgcgacag
     1081 ggcttttctg gaaaagtcta cctcacccga gcatcggccg atctcacgga gatcatgctc
     1141 gaagattcag cccgcgtaca agaaaacgac tgtcggtatg tcaacaaaca ggagcgcagg
     1201 cgcgggaaga cctgcattcg gccattttac gacggggacg aggtgcggaa gatcgtccga
     1261 aggtttgagg gagcacagta tggcgatcag ctgaagatcg cgcctcggat caccgcttcg
     1321 tttcatgacg ccggccatat tttgggatcg gctgcggttc gcgtgaaata tacggcgcgc
     1381 ggtaatacca ccacggtgtt gttcagcgga gatctgggtc gatcccatat gccgatccta
     1441 cgcgatcctg aaccgccgcc tccctgcgac gtcctgattc tcgaatctac gtatggcgac
     1501 cgatt
//
LOCUS       DASTWE010000726         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_310471, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000726 DASTWE010000000
VERSION     DASTWE010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310471"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VFT30_07530"
     CDS             <1..1479
                     /locus_tag="VFT30_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEU5092518.1"
                     /translation="TPEMDPVVESMDLVRAYLAASFEKRWDYAGEDPHEDARGFRVSG
                     LQQDKEEDEFAKVDAHRPFSFHEMKSDLVLPNGARFYNDHTHPEYSTPECRTIKDLLA
                     QDRAGERIILRAAQRRNQALGGGHVRLYKNNTDFHGHSYGCHDNYLVSRAVPFPTLVA
                     GLVPFLISRQVIAGAGKVGTEAQESGHVPGRYQLSQRADFMEAELGVDTMHNRPILNT
                     RDEPHADRRKYRRLHLIIGDANMCEYATALKVGTTRLVLDLIERGVAPEIELDQPVAA
                     VKQLSRDPDLKAAVRLKDGRTLSALDLQRHYHEAVYQEFAGTDEESDWLLTEWGETLH
                     LLANDRFQLVGKLDWVTKQWLLETFMREERIGWDDPWLASLDLEYHNVDPERGLYLGL
                     EADGKVWRMTTDEEIEKAIRNGPRDTRGGLRGLCVQRFSSHIKSLQWERVHFTGGFRS
                     RILDMGDLFAPDDVQRCAQIFTQAPSAVDALTLWNDGKDRQV"
BASE COUNT          378 a          394 c          483 g          331 t
ORIGIN      
        1 acaccggaga tggatccagt cgtggaatcg atggacttgg tgcgggcata cctagctgct
       61 tccttcgaaa agcgttggga ctatgccggt gaggatccgc acgaggatgc gcgtggcttt
      121 cgcgtttctg gtctacaaca ggacaaggaa gaagatgagt ttgcgaaggt ggatgcccat
      181 cgcccctttt cgttccatga aatgaagagc gatttggtgt tgccgaacgg cgcgcgattc
      241 tacaacgacc acacacatcc ggagtattcg acgccagagt gccgaacgat caaagatctt
      301 cttgcccaag atcgtgcggg agaacgtatc attctgcgtg ccgcccagcg tcgcaatcag
      361 gcgctcggtg gaggtcacgt taggctttac aaaaacaaca ccgattttca tggccatagt
      421 tatggatgtc acgacaacta tcttgtctcg cgggccgtgc cgtttcctac tcttgtagcg
      481 ggcttggtgc catttttaat cagtcgacaa gtgattgcag gtgcgggaaa ggtcggcact
      541 gaagcgcagg agtccggtca cgttccagga cggtatcagc tctcgcagcg cgccgacttt
      601 atggaagccg agttgggcgt cgatacaatg cataaccggc caattctcaa cacccgagat
      661 gaacctcacg ctgatcgtag aaaataccga cggctccacc tcatcatcgg cgatgccaac
      721 atgtgcgagt atgccaccgc gctgaaggtc gggacgacac gacttgtgct cgacctcatc
      781 gagcgagggg tggcgcccga gatcgagctg gatcaacctg tggctgcggt gaaacagttg
      841 tcgcgcgatc cggatctcaa agcagcggtc cgattgaaag atggacgcac actctctgcc
      901 ttggaccttc aaaggcacta ccatgaggcg gtctatcaag agttcgcagg taccgacgag
      961 gaatcggatt ggctgctgac ggaatggggt gagaccttac atctattggc gaatgaccgt
     1021 ttccaattgg tcggcaaact ggattgggtc acgaaacagt ggttgctcga gacattcatg
     1081 cgagaagaac ggatcggttg ggacgatccc tggctggcca gtttggacct ggagtatcac
     1141 aatgtagacc cggaacgggg actgtatctg gggttggaag cggatgggaa agtttggcgg
     1201 atgacgactg acgaggagat cgaaaaggcg attcggaacg gtccaagaga cacccgggga
     1261 ggactgcgtg gactctgcgt gcagcggttt tcgagccaca tcaagtcact tcaatgggaa
     1321 cgggttcact ttacaggagg gtttcgatcc cgaatcctcg acatgggaga tcttttcgcc
     1381 cctgatgacg tacagagatg tgcacagatc ttcacgcaag ctccgtcggc agttgatgcg
     1441 ctgacattgt ggaacgatgg aaaggataga caggtatgag atacaacatg acactggaac
     1501 ggcgcgaggg gccggtcgat cctatgccga agtcgcctcg acctcaagaa gaaggagggg
     1561 gacctcggcg accggatacc ggatca
//
LOCUS       DASTWE010000727         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_504729, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000727 DASTWE010000000
VERSION     DASTWE010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504729"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFT30_07535"
     CDS             <1..383
                     /locus_tag="VFT30_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEU5092519.1"
                     /translation="ADIARLKTRMDWKMPWYTITDSFDKDFGVDEWHGTNAFIRDGER
                     IFRTYFINNRGDEQMGNTWNYLDITALGRQEEWEDSPEGYPQSPPYKWWNWHDNYDAE
                     ASPDPKWVQISDVGEAAFRKRDAD"
     gene            complement(433..>1063)
                     /locus_tag="VFT30_07540"
     CDS             complement(433..>1063)
                     /locus_tag="VFT30_07540"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5092520.1"
                     /translation="LLIEKLPKNVLLVRSWPRPLMMMQGIDETFAGVIFIGYHTGTTN
                     QQGVRAHTISSARLADVRLKGVSVSEAGLNAAIAGHFNVPVIMVSGDDAVVKETTALL
                     GNIEGAVVKWASGFHSAKTLMPEAAYELIRAKVKTAIGRLKDFKPYKLATPIQLDVRF
                     KNYRPSELLAYLSIVERTDAHSIRFTGKDMIEVSKFLEFIMTYEPSLEP"
BASE COUNT          247 a          286 c          294 g          236 t
ORIGIN      
        1 aggccgacat cgcgcgcctg aaaacacgca tggactggaa gatgccgtgg tacaccatca
       61 cggacagctt cgacaaagac tttggcgtgg acgaatggca cggcacgaat gcattcatcc
      121 gcgacggcga gaggattttt cgaacttact tcatcaacaa tcgcggcgat gagcagatgg
      181 ggaatacttg gaactatctc gacataactg cgttggggag gcaggaggag tgggaagact
      241 cgccggaggg ttatccgcag tcgccgccgt acaaatggtg gaactggcac gacaactacg
      301 acgctgaagc atcacctgat ccgaagtggg tccagatttc cgacgttgga gaagctgcgt
      361 tccgaaagcg tgacgcggat tgacctcgag tttctctggc ctgtgcgttg aaatttacga
      421 ctcgccgtat gatcaaggct ccagcgatgg ttcgtaggtc atgatgaact ccaggaactt
      481 cgacacttct atcatgtcct tgccagtgaa cctgatgctg tgagcgtcgg tccgctccac
      541 gatacttagg tacgccagca actccgatgg acgataattt ttgaagcgca cgtccaactg
      601 aataggcgtc gcgagtttgt aaggcttgaa gtctttgagc cggccgatag cagtctttac
      661 ctttgcgcga atcaattcgt atgcggcttc gggcatcagg gttttcgctg aatgaaaacc
      721 cgatgcccac ttgacgaccg ctccttcgat attgcccagt agtgccgttg tctctttcac
      781 cactgcatcg tcaccggaga ccatgatcac cggcacgttg aagtgacctg caattgccgc
      841 gttgagaccg gcctccgaca cagaaacccc tttcagccga acatccgcca accgagcgct
      901 ggaaatcgtg tgggctcgca cgccttgctg attagtcgtt ccggtgtgat agccgataaa
      961 gatgacgccg gcgaaagtct cgtcaatgcc ctgcatcatc attaaagggc gcggccacga
     1021 gcgtacgagc agtacattct tcggcagttt ctcgatcagc agg
//
LOCUS       DASTWE010000728         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_407603, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000728 DASTWE010000000
VERSION     DASTWE010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407603"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..>1150
                     /locus_tag="VFT30_07545"
     CDS             98..>1150
                     /locus_tag="VFT30_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEU5092521.1"
                     /translation="MDIPRLQQKHSPGPRSAGRFSLRLKFMLFFSLILIMTCSSLSWY
                     FIETRHRVMTDHLEELGTILLTNTVRNEHFRIAGIVLEDRVTLDQFVQSLMAIDHVVY
                     VAVTASDGRILNQQSKLARRPVQGSHHVPAQPIYPDDRISKSLLAAPLTAPLMTRLVR
                     SAEQTLMPHDESSDWLLPFLVRTEVLYDFAMPVLREFSTSLPLLSDEFEVKPSAIPPT
                     KTVPVVGLVRIGLTDAQTKQALLIIARNVLILTGIIIVAGILGAHLLTSRITTPLRSL
                     AGAARQLAEGNDPPVPLVPVTHDEVGQLTQVFNVMTQSLYDRNRAITMNLDTIKRQVT
                     QLTTVHQASAAIASANM"
BASE COUNT          256 a          355 c          280 g          259 t
ORIGIN      
        1 gatcacggtg aacctcaaga ccgcgcggta tctcggcatc tcggttccga aagaactcga
       61 cagccttatc gacgaaacct attaggtgag tcggttcatg gatatcccgc gactccaaca
      121 aaaacacagt cccggcccaa ggtcggcggg tcgattcagt ctccgcttga agttcatgct
      181 gttttttagc ctcattctca tcatgacctg ttcatccttg agctggtatt tcattgaaac
      241 aagacaccgg gtcatgaccg accatctgga agaactggga accatcttgt tgaccaacac
      301 cgttcgaaac gagcattttc ggattgcggg catcgtcctg gaagatcggg tcacgctcga
      361 tcaattcgtg cagagtctca tggccatcga ccacgtcgtc tatgtggccg tcacggcatc
      421 cgacggtcgc atcctgaacc aacaaagcaa attagctcgg agaccggtgc agggatccca
      481 tcacgtgccg gcgcaaccga tctatccgga cgaccgaatt tcaaaatcac tgctggcggc
      541 tccgctgacc gctccgctca tgacccgcct cgttcgctct gcagagcaga ctttgatgcc
      601 tcacgacgaa tcctcagact ggcttctccc tttcctcgta cggaccgaag tcttgtacga
      661 ttttgcgatg cctgtcctac gggaattctc gacttccctg cctctacttt cggacgaatt
      721 tgaggtgaaa ccgagtgcca ttcccccgac gaaaactgtg ccggtggtcg gcctcgtgcg
      781 catcgggctc actgatgcac agaccaaaca agctttgctg atcatcgccc gcaatgtctt
      841 gatcctcaca ggaatcatta tcgtggcagg tatcctcggg gcacacctgc tgacgtcacg
      901 tatcacgaca ccgttgcgaa gcctggccgg agccgcccga caactcgccg aaggcaacga
      961 tccgccggtt ccgctcgtgc ccgttactca cgatgaagtc ggtcagttga cgcaggtgtt
     1021 caacgtcatg actcaatctc tctacgaccg caatcgtgcc attaccatga accttgacac
     1081 catcaaacga caggttacgc agttgaccac cgtgcatcag gccagtgccg ctattgcgag
     1141 cgccaatatg
//
LOCUS       DASTWE010000729         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_633794, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000729 DASTWE010000000
VERSION     DASTWE010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633794"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFT30_07550"
     CDS             complement(<1..252)
                     /locus_tag="VFT30_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092522.1"
                     /translation="MSFSERMIKIDRRIIFLVVGLCTLLPLLYPVGLPIKISSEVRGV
                     YDHIESLPERSVFLLSLDFDPASKPELHPQAIALLRHAFK"
     gene            complement(332..982)
                     /locus_tag="VFT30_07555"
     CDS             complement(332..982)
                     /locus_tag="VFT30_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092523.1"
                     /translation="MIFLRRQLPLLITLVTGLLFAAQYYVPHPASEEMLTSATKWLQI
                     IGGFALILGVTSLFHQHAVKIKRQEAGWGYSLVLYAGMLGTIAVGLWADGKEAVDGTM
                     TAFGWVYNFMMVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLVAAVIVMMGRVPL
                     GEYLIPVSGDVSSWILNVLNSSVRRAILIGVSLGAIALSFKIIFGVERSYLGGGKE"
     gene            complement(979..>2127)
                     /locus_tag="VFT30_07560"
     CDS             complement(979..>2127)
                     /locus_tag="VFT30_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEU5092524.1"
                     /translation="DVQVFDTPNDGGGSLTVQWAPAPWDGPAIRYQILVGEASATDPT
                     TFTIIAEFPANTMYVKDTKTAWWTRRSDKTWHQAIIKSAKGVEVKDDTPYAIAVATVQ
                     EERREFSPVQVATPAADWFNWNQLNNLIIAISFGGLVFYAISHAKRNNIFLRRIPGLD
                     AVDEAIGRATELGKPILYLTGAHDLHDPSTIAAAVILGKVAKRAALYETELIVPHREP
                     ITMAVCQEITKQAYLEAGKPDLFKEDANFFITADQFSYTAAVDGIMLRKKPAANFFMG
                     HYFAESLLLTETGASTGAIQIAGTDADHQLPFFVTTCDYTLIGEELYAASAYLSREPV
                     QIGTLRGQDIGKAVILSVLVVGTVLATIGVVTGSQWPQIFLDLFRDLK"
BASE COUNT          510 a          607 c          561 g          449 t
ORIGIN      
        1 tttgaaggca tgccgcagca acgcgattgc ttgcggatgg agctccggct tagaggcagg
       61 gtcaaaatcc agcgacagca agaagactga acgttccggt aatgattcga tgtggtcgta
      121 cacgccgcgc acctccgaag aaatcttgat cggcaaaccg accggataga gcagcggcag
      181 gagtgtgcag agaccgacca ctaggaagat aatcctccga tcgatcttga tcatgcgctc
      241 tgaaaagctc atcgtgggac cgttattggt gaatcgttaa tcgtgaatcg cacggatccc
      301 tcgtttaacg aaaaacgtcg aaccatgaac gtcattcctt ccctccacca agatacgatc
      361 gttccacgcc gaaaatgatc ttaaacgata aggcgatggc cccgaggctg accccgatca
      421 aaatggcccg ccgcacggaa gagttgagca cattcaaaat ccatgaagac acatcgccgc
      481 tcactggaat caaatactcg cccaacggca cccgtcccat catcacgatc acggccgcca
      541 ccaaaagcac cgccgcctca cgacttctgg ccctaaacga acggtaggca gccgaggcga
      601 tgaagaaagc caggatggcg aacatcgtac cttgcagcgg taccatcata aagttgtata
      661 cccagccgaa tgccgtcatc gtcccatcga cggcttcttt tccatcggcc cacagtccga
      721 ccgcgatcgt cccgagcata ccggcataca agacaaggct atagccccat cccgcttctt
      781 ggcgtttgat cttgaccgca tgttgatgaa acagactagt tacgcccaag atcaatgcga
      841 atcccccgat gatctgcagc cacttcgtgg ccgaagtgag catctcttcc gaagcgggat
      901 gcggcacata gtactgggcc gcgaacaaaa gaccggtgac gagagtgatt aagagaggaa
      961 gttgccggcg caggaaaatc atttgaggtc cctgaagaga tcgagaaaaa tctgcggcca
     1021 ttgcgaaccg gtgacgactc cgatcgtcgc taacaccgtt cccacgacga gtacgctcaa
     1081 gatcacagcc tttccaatgt cctgaccacg gagcgttccg atctgaaccg gttccctgga
     1141 cagatacgca ctcgcggcat agagctcttc accgatcaag gtgtagtcgc aagtggtcac
     1201 gaaaaatgga agttggtggt ctgcgtctgt tccggcgatt tgtatggcgc cggtgctcgc
     1261 gccggtttcg gtgagtagca gcgactcggc aaagtaatgt cccatgaaga aattggcggc
     1321 cggtttcttg cggagcatga tcccatccac cgccgccgtg tagctgaact gatcggccgt
     1381 gataaaaaag ttggcgtctt ccttgaagag atcgggcttc cctgcttcca aataggcctg
     1441 cttggtaatc tcctggcaga ccgccattgt aatcggctcc cgatgaggca ctatcagctc
     1501 cgtttcatac agagccgccc gtttcgcaac cttgcctaag ataactgcag cggcgatggt
     1561 tgaagggtca tgcaagtcgt gcgctccagt cagatagagg ataggcttgc ccaactcagt
     1621 ggcgcgtccg atcgcttcgt ccaccgcatc gagacctggg atacgacgaa gaaaaatgtt
     1681 atttcgtttc gcgtgactga tcgcgtagaa gaccagcccg ccgaacgaga tggcgatgat
     1741 cagattattg agctggttcc agttaaacca gtcggcggct ggtgtggcta cttggacggg
     1801 gctgaattca cgccgctcct cttgcactgt agcgactgca atcgcatagg gcgtatcatc
     1861 cttcacctcg acgcccttcg cactcttaat gatcgcctga tgccaagtct tgtccgatct
     1921 tcttgtccac caggcagttt tcgtgtcttt gacatacatg gtgttggctg gaaactcggc
     1981 gatgatcgtg aaagtcgtcg gatcagtcgc ggaggcttct ccgaccaaga tctgataacg
     2041 aatggctggc ccatcccacg gcgcgggagc ccactgcacc gtgaggcttc cccctccatc
     2101 gttcggcgta tcgaagacct gaacgtc
//
LOCUS       DASTWE010000730         1077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1280106, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000730 DASTWE010000000
VERSION     DASTWE010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280106"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /gene="ispE"
                     /locus_tag="VFT30_07565"
     CDS             complement(<1..780)
                     /gene="ispE"
                     /locus_tag="VFT30_07565"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU5092525.1"
                     /translation="MTKSVSRSTALPTSITVFAPAKINPILRILDRRPDGYHNLWSLM
                     QAVQLEDELALSLSDNHSTITLQCDEPSLKTDPSNLVYRAAEAVLEQSGQTVGLDMIL
                     AKRIPMGAGLGGGSSDAAATIAGLNRLLNLGWSKEKMAHVGQTLGSDVPFFFFAPSAI
                     VEGRGEKVAPVRIKGNRWVVLVNPGFPVETKWAYQQLSVNRAEVQPLSDVHAGLGKAS
                     ELSWEEVLQAAKNDFEATVFKAYPVLHKIKRELLAEGAETAL"
     gene            complement(773..>1077)
                     /locus_tag="VFT30_07570"
     CDS             complement(773..>1077)
                     /locus_tag="VFT30_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5092526.1"
                     /translation="LENLIERVVAFSMGGPVTDVEVRGWLHRPTTQSQQSAMPLDLTD
                     EGLDLEGLINGIEKDLLLKALERSKWVKKKAARMLRLNTRSFRYRLEKYAIKGGRD"
BASE COUNT          240 a          310 c          271 g          256 t
ORIGIN      
        1 taaagctgtc tcggccccct cagcgagaag ctcccgcttg attttatgaa ggacagggta
       61 agccttgaac accgtggctt caaaatcgtt tttggcggct tggaggactt cttcccatga
      121 gagctcagat gctttcccca gccccgcatg cacatccgaa agcggctgta cctctgcgcg
      181 gtttacagaa agctgctgat atgcccactt tgtttcgacc ggaaatccag gattaaccag
      241 gacgacccat cggttcccct tgattcgtac tggcgcaacc ttttcacctc gcccttccac
      301 aatcgcagaa ggagcgaaaa agaagaacgg gacatcgctt ccaagtgtct gaccaacgtg
      361 ggccatcttc tccttcgacc atcccaaatt caatagtcgg ttcagaccgg caatcgttgc
      421 agccgcatcg ctgcttccgc ctcccaaccc ggcccccatc ggaattcgct tggcgaggat
      481 catgtccaat ccaacggtct gtccgctctg ttcaagtacg gcctccgcag cacgatagac
      541 caggttggaa gggtccgtct ttaaggaagg ctcatcgcat tgcaaggtga tcgtcgaatg
      601 gttgtcgcta agggaaagcg cgagctcgtc ttccaactgc acggcctgca tcaaagacca
      661 gaggttatga taaccgtcag gccgacggtc cagaattcga aggatggggt tgatcttggc
      721 tggcgcgaaa acggtaatgg acgtaggtaa ggcggttgat cgtgaaacag acttagtcac
      781 gacctccttt tatagcatac ttctccaagc gataccgaaa cgatctggtg ttcagacgga
      841 gcatccgtgc ggctttcttt ttgacccact ttgatcgttc gagtgccttc agcaatagat
      901 ccttttcaat tccgttgatg agtccttcga ggtccaatcc ctcatcagtc agatcgagcg
      961 gcatcgccga ttgttgtgac tgcgtcgtgg gtcgatgaag ccatccgcgc acctcgacgt
     1021 cggtcacggg ccctcccatc gagaatgcga cgacccgttc gatcagattt tccaact
//
LOCUS       DASTWE010000731         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_795511, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000731 DASTWE010000000
VERSION     DASTWE010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795511"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFT30_07575"
     CDS             <1..653
                     /locus_tag="VFT30_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092527.1"
                     /translation="VAMNGAGIIHDFELAFMGHTSEEVDAEIDEGRFGMAEETGRILN
                     EAIARGAKDGQGLGEAIGYYMNQTADQFPNRAVSILSTGARLGIPVTVHVAVGTDIIH
                     MHPSADGAAIGATSLLDFRRLTAVVAGMEGGVYLNVGSAVILPEVFLKTLSLGRNLGH
                     PIANITTVNMDFLSHYRPQTNVVRRPTQKGGQGYSLTGHHEIMLPLLAAAVLEELG"
     gene            650..>1098
                     /locus_tag="VFT30_07580"
     CDS             650..>1098
                     /locus_tag="VFT30_07580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEU5092528.1"
                     /translation="MRPTPTAPGLPIPLSSEGLQARWQHLNTRYFAGSLKPIEIVWSQ
                     RLTASVGMFACRQGPKAFYPLAMTRGHREIRLSLPLFEQLAARTPYAEQELLNTLAHE
                     MIHQWQFDVLKRRPDHGLDFLRKMTQMNRSGEVAVTTYHSLEKEVIA"
BASE COUNT          241 a          319 c          309 g          229 t
ORIGIN      
        1 cagtcgccat gaacggggcc gggattattc acgacttcga attggccttc atgggtcaca
       61 cctcagaaga agtcgatgcc gagatcgacg agggtcgatt cggcatggcg gaggaaacgg
      121 gacggatcct gaatgaagcc atcgcgcgtg gcgcgaaaga cggccagggg ttgggcgagg
      181 ccattgggta ctacatgaac caaacagccg atcaattccc gaatcgcgct gtcagtatcc
      241 tgtccacggg tgcgcgactc ggcatccccg tgacggtgca tgtcgcggtc gggaccgaca
      301 ttatccacat gcatccctcg gcggacggtg ccgccattgg agctacttct ctcctagact
      361 tcaggcgcct caccgccgtc gtcgccggga tggaaggcgg cgtctatctg aacgtcggct
      421 cggcagtgat tcttcccgag gtgtttttaa aaacattgtc attaggcaga aacctcggcc
      481 atccgatcgc caacattacg actgtgaaca tggactttct atcgcactat cggccgcaga
      541 ccaatgtcgt acggcgaccc acgcaaaaag gtggacaagg ctattccttg accggtcacc
      601 acgaaatcat gctgccgttg ctcgcggcgg cggtgctgga ggaattggga tgagaccaac
      661 cccaaccgcc ccaggccttc cgatacccct ttcttccgaa gggctacagg ctcgttggca
      721 gcacctgaac acacggtact ttgccggttc actcaagccg atcgagatcg tctggagtca
      781 gcgcctgacc gcctcggttg ggatgtttgc ctgtcgccag gggccaaagg ccttctatcc
      841 tctcgccatg actcgcggtc atcgcgaaat tcgcctttct ctcccgctgt ttgaacagct
      901 cgccgcacgg acgccatacg cggaacagga actgctcaat accttggctc atgaaatgat
      961 ccatcaatgg cagttcgatg tgctgaagcg ccgaccagat catggcctgg attttttgcg
     1021 aaagatgaca cagatgaatc gatcaggaga agtggctgta acgacgtacc actcgctgga
     1081 aaaggaagtg attgcctt
//
LOCUS       DASTWE010000732         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1506606, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000732 DASTWE010000000
VERSION     DASTWE010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506606"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1202
                     /gene="plsX"
                     /locus_tag="VFT30_07585"
     CDS             165..1202
                     /gene="plsX"
                     /locus_tag="VFT30_07585"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU5092529.1"
                     /translation="MSTPSRMKIALDAVGGDHGPAPCVEGAIQAVKEFDVEVILVGDE
                     TTLKQECERQSSHDSRLTIRHASQVVEMHESPAAVARKKRDSSIWVATELVKNGHADA
                     VVSPGNTGASMVASFFVLGLIKGVERPAIAASLPTLSGEAIMLDVGANVDCTAKHLEQ
                     FALMGNEYGKHLLGKPNPRVGLLSIGEEDSKGNEVTKEAFKLLKGSSMNFVGNVEGRD
                     VYSGIADIVVCDGFIGNVALKISEGVAEMIKRLLLKEISGHFLGRLAYPLIAGPLMNL
                     KRKIDYAEFGGAPLLGVNGITMICHGRSSAKAIKNAIRRAKGMAEGRVRELIQRDIEE
                     SRSRPLMELET"
     gene            1199..>1725
                     /locus_tag="VFT30_07590"
     CDS             1199..>1725
                     /locus_tag="VFT30_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEU5092530.1"
                     /translation="MKACIAGTGSYAPARVLTNADLERMVATSDEWIRERTGICERRI
                     AATGEACSDLAVQAGKRALAAAGLAATDLDMILVATCTGDYPLPATACLVQHQLGATK
                     AAACDLSAACCGFIYALSVADAYVKNGMRHVLVIGSEVMSAITDWTDRNTCVLFGDGA
                     GAAIVSASDGERGILS"
BASE COUNT          366 a          439 c          543 g          377 t
ORIGIN      
        1 cattatacct gtttgaactg tggtacctat aagggtaagg cggtcattca agtcgaagag
       61 gcgtgagcag gttgtaccag tcggtccatt gcgcgagttt cccagcacgt ctggcgtgag
      121 cccagggggc gacgcgtgtt cagccagtca ttccaacacc atccgtgagt acaccatctc
      181 gcatgaagat cgcgctcgac gcggtggggg gcgaccatgg tcctgcaccc tgtgtcgagg
      241 gtgctattca ggctgtgaaa gaattcgacg tcgaggtcat tctcgtcggc gacgagacca
      301 cactaaaaca ggagtgtgaa cgtcagtcca gtcacgattc ccgcctcacc attcggcatg
      361 cttcccaggt tgtcgaaatg catgaatcgc ctgccgcggt tgctcggaag aaacgggact
      421 cgtcgatttg ggttgcaaca gagttggtca agaacgggca tgctgacgcg gtcgtgagtc
      481 caggtaacac cggggcgagc atggtggcgt cgttcttcgt cttaggactg ataaaggggg
      541 ttgagaggcc ggcgattgcc gccagcctgc cgacgttaag tggggaagcc atcatgctcg
      601 atgtgggggc taacgtcgac tgcactgcca agcatctcga gcaattcgcc ttgatgggca
      661 atgaatatgg gaagcatcta ctcggaaaac cgaatccccg tgtcggtctg ctgagcatcg
      721 gcgaagaaga tagcaagggc aacgaagtca ctaaagaggc gttcaagctc ctcaagggca
      781 gttcgatgaa tttcgtggga aatgtcgagg gacgcgatgt gtatagcgga atcgccgaca
      841 tcgtcgtctg cgatgggttt atcggcaacg tcgcgttgaa gatttccgaa ggtgtggccg
      901 agatgatcaa gcgactactg ctgaaggaaa tctctggcca ctttctcggc cgcctcgcgt
      961 atcccttgat tgctgggcct ctcatgaatt tgaagcgaaa gatcgactat gccgaattcg
     1021 gcggtgcccc gctgctgggc gtcaatggga tcacgatgat ctgtcatggc cgatcctcgg
     1081 ccaaggctat taagaacgcg atccgacgag cgaagggtat ggctgagggc cgtgtgcgag
     1141 agctgattca gcgagatatt gaagagagtc gctcacgtcc actgatggaa ctggagacat
     1201 gaaagcttgt attgccggaa ccggctccta cgcgcctgcc cgggtcctga cgaatgcgga
     1261 tctcgaacgg atggtcgcca cctccgatga atggattcga gaacggacag ggatctgtga
     1321 gcgacgtatc gcagccaccg gagaagcttg ttcggatttg gcggttcagg ccggaaagcg
     1381 ggcgttggct gctgccggtc tggcagcgac tgatctcgac atgattttgg tcgccacctg
     1441 tacgggtgat tatcctcttc ctgcaacggc ctgtctcgta caacatcagc tcggtgccac
     1501 caaggccgcg gcgtgcgatc tttcggccgc ctgctgtgga tttatctatg cgctctcggt
     1561 ggcggatgcg tatgttaaaa atggaatgcg ccatgtcctg gtgatcggat cagaggtgat
     1621 gtccgccatt accgactgga ccgaccggaa cacctgcgtg ctatttgggg atggggctgg
     1681 cgcggccatc gtcagtgcca gcgatggaga acgagggatt ctctc
//
LOCUS       DASTWE010000733         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1054325, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000733 DASTWE010000000
VERSION     DASTWE010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054325"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1963
                     /locus_tag="VFT30_07595"
     CDS             65..1963
                     /locus_tag="VFT30_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5092531.1"
                     /translation="MKVEYSKGNRASKELIQLHRRTSFEASGRKMKVMLIFPPDWYPS
                     EPYLSLPSLTSVLRQAGHTVIQKDINCEMWDWYFSEEFLKKVLRRVPQQLDRLRKLAK
                     KRDLTEWEMDVQLALCDVTRPRMEKLIRDAEEAKHIVRNEKFYDIDRLEWAIQVFREV
                     TSVISLVYAPARICMPPMETDLSYKPYVSAEVMDAVQDEQVNVYRDVFDYLVKPVIQT
                     EQPDIVGISIVLQQQMFSSMTFCALIKQHFPHIHITIGGNTVTRLRDVLPASPLFQYF
                     DSAVVYEGETAFTQLVEAVGAKRSLANVPNTIYKDETGIHVSPLSYAEDMAALPPPDF
                     DGLPLEKYFVPTLVLPYLATRGCYWGRCEFCDHGEGYTAGYRSKKIQDILADITYLRD
                     KYGTKHFHFTDESYPPALFRKLSRGLVESNMGIFWTTHMRFEKSLLEDAVWRDAKESG
                     CRYLHFGYESGVERVLQLMDKATTAEVMTKHLKLTAEAGIWNHCMGFFGFPGETKEEA
                     WQSVEFLEQNKDHVHSLGFGTFDLGRHNPVAKHPEKWGVTAYKNPDWDLALDYYYTVK
                     NGMSIEEAERVFEQFERNHNPGWDLRLYIREYIFLYISRFGLEKLPDLQYQAAKISGV
                     THTLAGKM"
     gene            1998..>2391
                     /locus_tag="VFT30_07600"
     CDS             1998..>2391
                     /locus_tag="VFT30_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEU5092532.1"
                     /translation="MSVNGLVQIDGLTPVEKEERKTSKVMLIFPPEWVPTAPYLALPS
                     LTAVLREAGHTVIQRDINIGMWDHFFSLEFLIWVKARLGMQLKVLQDKEKARLLTERD
                     VDQKAAVERAYDVDVFDLAEQAEDAKSIV"
BASE COUNT          550 a          588 c          718 g          535 t
ORIGIN      
        1 tcttcaacct aactcgttgg ctttccagtt gtttaaacag cttgaagaag gcaggtaggc
       61 tgggatgaaa gttgagtatt caaagggaaa tcgggcgtcg aaggaactga ttcagctcca
      121 tcgccggacc tctttcgagg ccagcggccg gaagatgaag gtgatgctga tcttcccgcc
      181 ggattggtac ccctccgaac cttatttgag ccttccctcc ctcacctccg ttctccgcca
      241 agccgggcac acagtcattc aaaaagatat caactgtgag atgtgggact ggtacttcag
      301 tgaggagttt ctgaagaagg tcttgcgtcg ggtgccgcag caattggatc ggctccgcaa
      361 gctggcgaag aagcgggacc tgacggagtg ggagatggat gtgcagctgg cgctctgcga
      421 tgtcacgcgt ccacgaatgg aaaaattgat ccgtgatgcg gaagaggcga agcacatcgt
      481 cagaaatgag aagttctacg atatcgacag gttggaatgg gcaattcaag tctttcgaga
      541 ggtcacatcg gtcatttcac tggtctatgc cccggcgcgg atctgcatgc cgccgatgga
      601 gacggatctg tcatacaagc cgtacgtctc ggccgaggta atggacgcgg tgcaagatga
      661 acaggtcaac gtataccggg atgtgttcga ctatttggtc aagccggtga tccaaaccga
      721 gcagccggac atagttggga tctcgatcgt gctgcagcag cagatgtttt cctccatgac
      781 cttctgtgcc ctcatcaaac agcactttcc ccacatccat attacgatcg gtggcaatac
      841 ggtgacccgc ttgcgcgatg tgctacctgc ctcgccgctc tttcagtatt tcgacagtgc
      901 cgtagtctac gaaggggaga cggcgttcac gcaattggtc gaggcggttg gcgcgaagcg
      961 gagtttggcc aatgtgccga acacgatcta taaggacgag acgggcattc atgtttcccc
     1021 gctgagttat gctgaagata tggctgcatt gccgccgccg gattttgatg gattgccact
     1081 ggagaagtat ttcgtcccga cccttgtatt gccctatttg gcgacgcgcg ggtgttactg
     1141 ggggcgctgt gagttctgcg accatggcga gggctatacc gccggctatc ggtcgaagaa
     1201 aattcaggac attctcgccg acatcaccta tctgcgcgac aagtacggca ccaaacattt
     1261 tcactttacc gacgaatcct atccgccggc gctgttccgc aagctctccc gaggattggt
     1321 cgagagcaac atgggaatct tctggacgac gcatatgcgg ttcgagaaga gtctgttgga
     1381 agacgccgtc tggcgggatg cgaaggagtc gggctgccgc tatctgcact tcggctacga
     1441 atcaggagtc gagcgggtgc tgcagttgat ggacaaagcg acgacagcgg aggtcatgac
     1501 gaagcatctc aagctgacgg cggaggcagg gatttggaat cactgcatgg gcttcttcgg
     1561 ttttcccggc gagacgaagg aggaggcttg gcagtcggtg gagtttctgg agcagaacaa
     1621 ggaccatgtc cattcgctgg ggttcggtac gttcgacctg gggcgacata atccggtggc
     1681 caagcatccg gagaagtggg gcgtgacggc atataagaat ccggattggg atctggcgct
     1741 ggactactat tacacggtga agaacggcat gagcatcgaa gaggcggagc gggtgttcga
     1801 gcagttcgaa cggaaccaca atcccggttg ggatttgcgc ctctatatcc gagaatatat
     1861 ctttctttat atctcaaggt ttggattaga gaagctgcct gacctgcagt atcaagccgc
     1921 gaaaatatca ggagtcacac acacattagc cgggaagatg tgatctcgtg atgtggactg
     1981 actagttgga taaaacgatg agtgtgaatg gtctcgtaca aatcgacggc ttgactccgg
     2041 tcgagaaaga agagcgcaaa acctccaagg tcatgctcat ctttccaccc gagtgggtac
     2101 cgacggcgcc ataccttgcc ttaccttcgc tcacggccgt cttgcgcgaa gccggccata
     2161 cggtcatcca gcgcgatatc aacattggaa tgtgggatca cttcttcagc ctggagtttc
     2221 tgatctgggt gaaggctcgc ttgggcatgc agctcaaggt tttacaagac aaggagaagg
     2281 caaggctgct cacggaacga gatgtggacc agaaagctgc cgtggagcgg gcgtatgacg
     2341 tggacgtgtt cgatctggcc gagcaggctg aagatgccaa gtcgatcgtc c
//
LOCUS       DASTWE010000734         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_924932, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000734 DASTWE010000000
VERSION     DASTWE010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924932"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1098
                     /locus_tag="VFT30_07605"
     CDS             <1..>1098
                     /locus_tag="VFT30_07605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5092533.1"
                     /translation="GIYNGAVRFDNVFVQVADRLGDEGEGLRRALESLTVGRLTLPAA
                     CLGNLKQCLWLARLRAQQRIQYDRPIGEHTDIGSKIARMASRTLALEALVTITGIWAD
                     AKIDVRLESAAAKILATEWLLESVLDLFRIYGGRGFETSESLRLHGDLPAPVERMIRD
                     SLINVIWEGTNGILTLWIGREGLAEYVSQGKAFLEFQISEMAQSLPFFAKVAGRSFNR
                     LPRIEGWPASAASESAWERFVTKKSRELAQKSLWAAVRHRQGLARKQLLTTRLVKAGM
                     QLFAVESVLWYVAQQDVRSHQLTEELLQHFCSQVEEEFNPIPLLSLRRSLWQDDSHVF
                     RLAQDILAGKAEWLEEGILAWHPVGHGRGDQF"
BASE COUNT          229 a          304 c          323 g          242 t
ORIGIN      
        1 ggcatctaca acggcgccgt ccggtttgac aatgtcttcg tccaagtggc cgaccgttta
       61 ggagatgagg gagaagggtt acggcgagcc ttggaaagtt tgaccgtagg ccggcttacc
      121 cttcccgcag cgtgcctcgg aaacctgaaa caatgcctct ggctcgctcg gctccgcgcg
      181 caacagcgca tccaatacga ccgaccgatc ggcgagcaca cggacatcgg atcgaaaatc
      241 gcgcgcatgg catcacgcac cctcgcactg gaggcgttgg tcaccatcac agggatttgg
      301 gccgatgcca agatcgatgt gcggcttgaa tcggccgccg ccaagattct cgcgaccgaa
      361 tggctgctcg aatctgtgct cgatcttttc cgcatttatg gcggacgcgg attcgaaacg
      421 tcggaatcgc tccggcttca cggagacctg ccggcaccgg tggaacgcat gattcgcgac
      481 tcgttgatca atgttatttg ggagggaacg aacggtattc tcaccctctg gatcgggcgc
      541 gagggattgg ctgagtacgt atcccaaggt aaagccttct tggaattcca gatcagcgag
      601 atggcacagt ctctgccgtt tttcgccaaa gtcgcgggtc gttccttcaa ccgcttgcct
      661 cgaattgaag gatggcccgc ttctgccgcg tctgaatcgg catgggaacg gtttgtcacg
      721 aagaagtccc gagaacttgc acagaagtcg ctatgggccg cagttcgcca tcgtcaaggt
      781 ctcgcgcgta agcaattgtt gacaacgcgc ctggtcaaag ccggaatgca actctttgcg
      841 gtggaatcag tattatggta cgtagcgcaa caggacgttc gaagtcatca gttgaccgaa
      901 gaactcttgc agcacttttg ttcacaggtc gaagaggagt tcaatcccat tcccttgctc
      961 tctctcaggc gatcgttatg gcaggacgat tcgcatgtgt tccgcttggc gcaggatatt
     1021 cttgccggca aggccgagtg gttggaagag ggaatccttg cctggcaccc cgtcgggcac
     1081 ggtcggggcg accagttc
//
LOCUS       DASTWE010000735         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_504931, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000735 DASTWE010000000
VERSION     DASTWE010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504931"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..448
                     /gene="rpsH"
                     /locus_tag="VFT30_07610"
     CDS             53..448
                     /gene="rpsH"
                     /locus_tag="VFT30_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5092534.1"
                     /translation="MVTDPIGDLLVRLKNGAQRRHETVTVPTSKLKRAILEILRQEGY
                     VDAIEDGVHDGHPILTVRLRYVGEGQPMITGLERVSKPGRRVYVGSQDIKKVRNGIGV
                     SILSTSKGIMTDQESRKGRLGGEVLCSVW"
     gene            492..1028
                     /gene="rplF"
                     /locus_tag="VFT30_07615"
     CDS             492..1028
                     /gene="rplF"
                     /locus_tag="VFT30_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU5092535.1"
                     /translation="MSRIGKKPIAIPGGVEVKVAGSTVSVKGPLGKLDWSLVHGVDVA
                     VNNGHVVVGRSSDDRKLRALHGLTRAELSNMIYGVTKGYERSLEITGVGYKTQIQGRT
                     LSFNVGYINPVTYEVPTGIDVKVDKQTLINIKGVDKRLVGQVAADLRAIKPPDVYKQK
                     GVRYAGETLRKKEGKTGK"
BASE COUNT          287 a          234 c          344 g          248 t
ORIGIN      
        1 tagctggtgt tctcacgacg gtgactgcca atggaggaga gaaggatata gcatggttac
       61 cgatccaatc ggcgatcttc ttgttcgttt aaagaatggc gctcagcgcc gtcatgagac
      121 agtcacggtt ccgacctcaa agctgaagcg tgccattttg gagattttga ggcaggaagg
      181 ctatgttgat gctatcgagg acggagttca tgatggacat cccattctga ctgtgcgtct
      241 tcgctatgtg ggagagggac agcctatgat caccgggttg gaacgcgtca gcaagccggg
      301 gcgccgagtc tatgtcggca gtcaggatat caagaaagtg cgcaatggga tcggtgtgtc
      361 catcctttct acatcaaaag gcatcatgac cgaccaagaa tcccgtaagg gtcgtcttgg
      421 aggtgaggtt ctgtgctcgg tatggtaggc agatgagtca tttcgatctc gagtaaaagg
      481 tataacaaac catgtcccgc attgggaaaa agccgattgc aattccgggt ggagtggaag
      541 tcaaagttgc cggatcaacc gtgtccgtca aaggcccatt aggcaagctg gattggtcac
      601 tcgtgcacgg ggtagacgtt gccgtcaata atggtcatgt cgttgtcggg cgttcgagtg
      661 atgatcgtaa actgagagcc ttgcacgggt tgacgcgtgc ggaattgagc aacatgatct
      721 atggggtgac caaggggtac gaacgttcac ttgaaatcac tggagtgggc tacaagaccc
      781 aaatccaggg ccggacatta agtttcaatg tgggctatat caatccagta acctatgagg
      841 ttccgactgg gatcgacgtg aaagtggaca aacagacgct catcaacatc aagggagttg
      901 ataagcgctt ggtgggtcag gtggcggctg atctgcgggc cattaagccg ccggatgtct
      961 ataagcaaaa aggtgttcga tacgcgggcg agacattgcg caagaaagaa ggcaagaccg
     1021 ggaaatagga attggctatg aatgctgcag acaaagttcg acagctcaat cgacggcgcc
     1081 gacgagtgag gcgtgtgatt atgggaacgg cag
//
LOCUS       DASTWE010000736         1367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_246621, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000736 DASTWE010000000
VERSION     DASTWE010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246621"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFT30_07620"
     CDS             complement(<1..685)
                     /locus_tag="VFT30_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5092536.1"
                     /translation="MAKQKYDRSKPHLNVGTMGHIDHGKTTLTAAITKVSQMLGNAEF
                     RAYDQIDNAPEEKARGITINIAHVEYQTEKRHYAHVDMPGHRDYIKNMITGAAQVDGA
                     ILVVAAPDGPMPQTREHVLLARQVEVPSIVVYLNKVDQMNDPELLELVELELRELLSG
                     YGFPGDSTPIVRGSAKEALDSTSTDPNAPEYQSIRELLRVVDEYIPEPKRETDKPFMM
                     AVEDVFSIKG"
     gene            complement(723..>1367)
                     /gene="fusA"
                     /locus_tag="VFT30_07625"
     CDS             complement(723..>1367)
                     /gene="fusA"
                     /locus_tag="VFT30_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449074.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5092537.1"
                     /translation="KPRVAYRESITRAVPKVDFKYAKQSGGHGQYGHVVISLEPGERG
                     SGIIFENKIVGGAIPKEFIAPVQKGVMEAADSGVLAGYPVVDLKITLFDGSFHEVDSS
                     EMAFKMAGSMAFKEGVQRGSPVLLEPVMKVEVVVPDEYLGEVIGQLNSRRGSIQGLEV
                     RPGNAQAVRAHVPLGEMFGYATDLRSATQGRGVSSMEFDHYAPVSQSVAQEILK"
BASE COUNT          273 a          448 c          321 g          325 t
ORIGIN      
        1 gtcccttgat cgagaacacg tcttccaccg ccatcatgaa cggcttgtcc gtctcgcgct
       61 tcggctcggg gatgtactca tccaccaccc gcagcagctc acggatgctc tggtactccg
      121 gcgcattcgg gtcggtgctc gtgctgtcca gcgcttcctt cgcactgcca cgcacgatcg
      181 gggtgctgtc gccggggaac ccgtagccac tcagcagttc acgcagctcc agctccacca
      241 actccaacag ctccggatcg ttcatctggt ccaccttgtt caggtacacc acaatactcg
      301 gcacttccac ctggcgagcc agaagcacat gctcgcgcgt ctgcggcatc ggtccatccg
      361 gggccgccac caccaggatc gcaccatcca cctgggccgc tcccgtgatc atgttcttga
      421 tgtagtcccg gtgtccgggc atgtccacat gggcatagtg acgcttttcc gtctggtatt
      481 ccacatgtgc aatgttgatc gtgatgccgc gcgctttttc ctcgggtgca ttatcgatct
      541 gatcgtacgc tcggaactcg gcgttcccca gcatctgcga cactttcgtg atcgccgccg
      601 tcagcgtcgt cttcccatga tcgatatgcc ccatcgtccc cacattcaaa tgcggcttgc
      661 tcctgtcata tttctgcttc gccattgatg cgtctccttc taataatgaa agcgttcgtt
      721 atttatttca aaatttcctg cgccacagac tgcgaaaccg gcgcatagtg atcgaattcc
      781 atgctggaaa ctccgcgtcc ctgtgtggcg gagcgcaaat ccgtagcata gccaaacatt
      841 tcgcccaatg gaacatgggc gcggacggct tgcgcgttac ctggacgaac ttccaacccc
      901 tgaatgctgc cacgacgact attcagttga ccgatgacct caccaaggta ttcatccgga
      961 accaccactt cgactttcat gaccggctcc aacagtacag gtgaacctct ctgcacacct
     1021 tccttgaacg ccatggagcc tgccatcttg aaagccattt cgcttgagtc tacttcgtgg
     1081 aatgaaccat cgaacaacgt gatcttcagg tccacgacgg gatagccggc taatacgccg
     1141 ctatctgccg cttccatcac acccttttgg accggcgcga tgaattcctt cgggatcgcg
     1201 ccgccgacaa ttttgttttc gaaaataatt ccactgcccc gttctccggg ctccagcgaa
     1261 atcacaacgt ggccgtactg accgtgacca ccagactgtt tcgcatactt gaaatcgact
     1321 ttcggaacag cccgagtaat ggactcacgg taagcaaccc gcggctt
//
LOCUS       DASTWE010000737         1294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_472647, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000737 DASTWE010000000
VERSION     DASTWE010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472647"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..>1294
                     /locus_tag="VFT30_07630"
     CDS             135..>1294
                     /locus_tag="VFT30_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEU5092538.1"
                     /translation="MLIGEDHAPIASGSHEWENRYENGIWTYNLEDVWTGLRESYRML
                     NNEVVEQCNTPIQTIGAIGFSAMMHGYMAFDTQGNLLVPFRTWRNTITAQAAEELTDL
                     FQFNIPQRWSIAHLYQAILNKESHIKGISHLTTLAGYVHWKLTGKKVLGVGEASGMFP
                     IDSTTNDYDERKIALFNDRLKAETIAWQLRDILPKVLVAGDAAGALTAEGARLLDPAG
                     QLQAGIPLCPPEGDAGTGMVATNSVGERTGNVSAGTSVFAMIVLEKPLSKVYPEIDMV
                     TTPTGKPVAMVHSNNCTSDLNAWVDLFQEFTHALGVEMSESRLFEMLYSQALAGDADG
                     GGLLAYNYFSGEHITHLEEGRPLFVRTPESRFTLANFMRVHLFAALGALKMG"
BASE COUNT          319 a          347 c          369 g          259 t
ORIGIN      
        1 aaagcattcg acgagccaac agcatccgtg aaacgctatg ggagctgaac atgaatctac
       61 aagacaccca gcgggcaatc cagagctgaa aaaccgctct tggcatcgag ttcggctcaa
      121 ctcggatcaa ggccgtgctg atcggcgagg atcatgcgcc gatcgcatcg ggaagccatg
      181 aatgggaaaa ccgatacgaa aacggcatct ggacatacaa tctcgaggat gtttggacag
      241 gcttgcggga aagctatcgg atgctcaata acgaggttgt tgagcagtgc aacaccccga
      301 tccagaccat tggtgcgatc ggttttagcg ccatgatgca cggctatatg gcattcgata
      361 cccagggaaa tctactcgtc ccgtttcgca catggcgaaa cacaatcacc gcacaggcag
      421 cagaggagct cacggatctg ttccaattca acattcccca gcgttggagc attgcccatc
      481 tctaccaggc gatattgaat aaggaatccc atatcaaggg tatcagccat ctcaccacgc
      541 tagcaggcta tgtccattgg aaactgacgg ggaaaaaggt attgggcgtt ggcgaagctt
      601 cgggcatgtt ccccatcgat agcacgacta atgattacga tgaacggaag atcgcgctat
      661 tcaacgaccg gctcaaggcg gaaacgattg cgtggcaact acgggacatc ctgccgaaag
      721 tccttgtcgc aggagatgcc gcgggcgctc tgaccgctga aggcgcaaga ctgctcgatc
      781 cagcgggaca gctgcaagcg gggatcccgc tctgtccgcc cgagggcgac gccggcactg
      841 gcatggtcgc gactaatagc gttggagagc gaacgggcaa tgtatcagcg ggaacatccg
      901 tctttgccat gatcgttttg gagaagccgc tgtcaaaagt atatcccgag attgacatgg
      961 tgacgacgcc cacaggcaaa cctgtcgcca tggtccatag caacaactgc acatccgatc
     1021 tcaatgcctg ggttgacctg ttccaggaat tcacgcatgc gcttggggtg gagatgagcg
     1081 aatccaggct gtttgaaatg ttgtatagcc aggcgctcgc aggcgatgcc gatggcggcg
     1141 ggctgctcgc ttacaattac ttttcgggcg agcatatcac gcaccttgaa gaagggcggc
     1201 ccctgttcgt ccgtaccccc gaaagccgct tcacgctggc gaacttcatg cgggtgcatc
     1261 tgttcgcggc actcggtgca ttgaagatgg ggtt
//
LOCUS       DASTWE010000738         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1215529, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000738 DASTWE010000000
VERSION     DASTWE010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215529"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1063
                     /gene="ppk1"
                     /locus_tag="VFT30_07635"
     CDS             <1..>1063
                     /gene="ppk1"
                     /locus_tag="VFT30_07635"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU5092539.1"
                     /translation="RLELDAATPSHIREILTRNLQLPLYLVYTGDSPIGMAGIRELYA
                     LERPDLKDPPFIPSMPSYMNKSGSLFATIRQQDVLLYHPYDSFTPVVEFIREAANDPD
                     VLAIKQTLYRVNPKSPIVDALMEARVNGKQVAVLVELKARFDEENNIEWARKLEDEGV
                     HVVYGVRGLKTHAKVCLVVRREQDGIYRYIHLGTGNYNVVTSRIYTDVSYFTCNADIT
                     RDIADLFNSLTGYSRKNSYSKLLVAPTTLRQQLLDRIERETCRHRQQGDGYIAFKMNA
                     LVDTQCIQALYRAAQAGVRIDLQVRGICCLRPGLPGISETITVTSVVGRFLEHARIYY
                     FRHGGQHEVLIGSADLMPRN"
BASE COUNT          244 a          266 c          292 g          261 t
ORIGIN      
        1 acgattggaa ttggatgcgg caacgccgtc ccacattcgc gagattctga cgcgaaacct
       61 tcagctccca ctgtaccttg tctatacggg agacagccct atcggcatgg ccggaattcg
      121 tgaactgtac gcgcttgagc gtcccgatct gaaggaccct ccgtttattc catccatgcc
      181 ttcgtatatg aacaaatcag gcagcctttt cgccaccatc cgtcaacagg atgtgctctt
      241 atatcatccc tatgatagct tcacgccggt cgtggagttt attcgggaag ctgcaaacga
      301 tccagacgtt ctcgccatta aacagaccct ctatcgcgtt aacccgaaat caccgattgt
      361 cgatgcgttg atggaagctc gtgttaacgg caagcaagtc gcggtcttgg ttgagctgaa
      421 agcgcgcttc gacgaggaga ataatattga atgggccagg aaacttgaag acgaaggggt
      481 gcacgtcgtg tatggtgtgc gcgggctcaa gacgcatgcg aaggtgtgtc tcgttgttcg
      541 acgcgaacaa gacggtatct atcgctatat tcatctcggc acgggaaact ataacgtggt
      601 caccagtcgt atctataccg atgtgagcta tttcacgtgc aatgccgaca ttacccgcga
      661 tatagccgat ctgtttaact cgctgacggg ctactctcgc aagaattctt attcgaagct
      721 actcgtggca ccgacgacgc tcaggcaaca attactggac cggatcgagc gcgagacgtg
      781 ccgccaccgt cagcagggtg atgggtatat tgcgttcaaa atgaacgcat tggtggatac
      841 gcagtgtatt caagcactgt accgtgcggc gcaagccgga gtccggattg atcttcaggt
      901 aaggggaatt tgttgtctga ggccaggact tccgggtatc agtgagacga tcacggtcac
      961 ttcggtggtt ggtcgttttt tggagcacgc ccggatctat tattttcgtc acggtgggca
     1021 gcatgaagtg ttgatcggga gcgccgattt gatgccgcgc aat
//
LOCUS       DASTWE010000739         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1539012, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000739 DASTWE010000000
VERSION     DASTWE010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539012"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(548..829)
                     /locus_tag="VFT30_07640"
     CDS             complement(548..829)
                     /locus_tag="VFT30_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU5092540.1"
                     /translation="MVVGLCTIELFIPGSLSLKDKRQVIHGLKDMLRGKFNLSVAEVD
                     GQDLWQKAILGMACVSNESSHVSQVLEQALNLIKSMPAVEVVRTQLELL"
     gene            complement(940..>3170)
                     /gene="infB"
                     /locus_tag="VFT30_07645"
     CDS             complement(940..>3170)
                     /gene="infB"
                     /locus_tag="VFT30_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5092541.1"
                     /translation="PTVESERQAQPVSSIGVPTQTPSTEHASGGLVDRTLPTEESPNE
                     ISLLAQVPLVAKQEEMPAKPVVAPSTIGATTPEPVTAKKKSMAFEAIEAEAQKDKLKK
                     ARKVGRPKDDQQQDVKFREDAARWQDLRAIPVQRRDDRSKHVHHSTPAEITKPRRKSV
                     KITPRTTVKEFAELIGQRPADIVRKLMDMGQMLTFHQPMNVDAASIFAEESGVKIEIS
                     VEKAGDELLQDVVETGGDERPEPRPPVVTIMGHVDHGKTSLLDAIRQTNVAEGEAGGI
                     TQHIGAYTVSVRGKQVTFLDTPGHEAFTAMRARGAKVTDIVILVVAADDGVMPQTIEA
                     IRHAKAAGVPLIVAMNKIDKPTANPDRVKNALSEHGLISEAWGGDTIMVEVSAKQKTG
                     LDTLLEMILLQAEVLELKADPHRQAKGTVIEAKIERGRGPVATVLVQSGTLRVGDAYV
                     VGTFSGRVRALINDRGEKAQQAGPSIPVEVIGLPGVPSAGDVFHVVSNERVAREIAEE
                     RAQKRRAAELTGPAKVTLDDLFAKIQEGSVKELAIVIKADVQGSSEALAGAVEKLSTD
                     AVKLRVIHNGVGGVMESDVLLAAASRAIIIGFNIRPEPKATALAEQQGVDIRLYTIIY
                     DAIADIKAAMEGLLEPTLKERVLGRAEVRQVFTIPKIGAVAGSYVIDGTISRASAGVR
                     VIRDNVVVYQGKFASLRRFKDDVREVQQGYECGLSIENFNDVKAGDIIEAYAIDKIAT
                     KL"
BASE COUNT          626 a          949 c          803 g          792 t
ORIGIN      
        1 atcccgctca ccctcgcccg tttccatggt cgtgacaaaa acatgagcga tgcgcagatc
       61 ccctgtcagc tcgacatcgg tgaccgtcac gtcacgaacc ctgggatcct tgatttttcg
      121 catgagaata tccgccactt ccatccggat ctggtcggcc acccgatctg ctcttttata
      181 ggtcgtcttc gacatcttgt tataccgacg ttgtcgatcg ccagcattgc gagcctagca
      241 ggctgcggaa aaactcggtt gatgcggaca atatagctgg aattggccgt acagtgccac
      301 cgccagaata tcttcaggat gctcaaaaag gttgctgttc tcacctgccc ggcccaggcg
      361 cgccaagacg cgcctctccg caggcaaggc cgcaacaagg tccgcgagga gacgaagaag
      421 aagcctcagg tcgcggacgg gcgcgaactg gtgagcttcc agtatcctag gagacgaggc
      481 atactttatc catacgttga acctacgcga tgcgagaacg ccgttggcgg actttttcag
      541 catcctgcta gagcaattct aattgagttc tgaccacttc gactgccggc atgcttttga
      601 tcagattcaa cgcctgttca agcacctggc tcacgtgact actttcattc gagacacaag
      661 ccattcccag aatggccttc tgccagagat cctgcccgtc tacctcggcg actgagagat
      721 taaattttcc ccgaagcatg tccttgagcc cgtgaatgac ctgtcgcttg tctttaagcg
      781 ataggctccc tgggatgaat aattctatcg tgcatagacc cacgaccatc gccaagggtt
      841 cggttagagt aggctcgtga tgaaggccac gattccgctt actgaacgcg ccagatctct
      901 gggcaatggg cgccgcccta aaaagtctga aggttctacc tagagctttg tggcgatctt
      961 atcgattgca taggcctcga tgatgtcgcc ggctttgaca tcgttgaagt tctcgatact
     1021 caagccacat tcatacccct gctgcacttc acgcacatcg tccttgaaac gccgcaacga
     1081 agcgaattta ccctgataga ccaccacatt gtcgcgaatc actcgcaccc cggcgcttgc
     1141 ccgagatatt gtcccgtcaa taacataaga cccggccacc gctccgatct tcggaattgt
     1201 aaacacctgc cggacttccg ctcgtcccaa tacgcgctct ttcagggtag gctcgagcaa
     1261 gccttccatc gcggccttga tgtcggcaat ggcgtcataa atgatcgtgt agagcctgat
     1321 atcgacaccc tgctgttcgg ccaatgcggt tgccttcggc tccggcctga tattgaaccc
     1381 gatgatgatg gctcgtgatg cagcggcaag cagcacgtcg gattccatga ctccaccgac
     1441 tccattatga atcacacgga gcttgacggc gtctgtcgac agcttttcga ctgcgcctgc
     1501 caatgcttca gacgatcctt gtacgtcggc cttgatgaca atggccaatt ccttcacgga
     1561 tccttcttgg atcttcgcga agagatcatc cagggtaacc ttcgcaggac cggtcagttc
     1621 tgctgcccgc cgcttttgag ctcgttcttc cgctatctct cgggcgactc tctcattgga
     1681 gacgacatga aagacatctc ccgctgacgg aacgcccggc aatccgatta cttccaccgg
     1741 aatggacggt cctgcctgct gtgctttttc gccacgatca ttgatgaggg cccggacacg
     1801 cccactgaat gttcccacga cataggcgtc acccactcgt agggtcccgc tctggaccaa
     1861 caccgtcgca acggggcccc tcccccgttc aatcttggct tcgatgacgg tgcctttggc
     1921 ctgtcggtgt ggatccgcct tgagttcaag cacttccgcc tgaagcaaaa tcatctcgag
     1981 gagagtatcg agccccgttt tctgtttggc ggatacctcg accatgatgg tatccccgcc
     2041 ccatgcttcg gagatcagtc catgttcaga gagcgcgttt tttacccgat caggattggc
     2101 ggttggctta tcgatcttat tcatcgccac gatcagcggc actccggctg ccttggcatg
     2161 gcggatcgct tcgatggttt gcggcatcac tccgtcgtct gcggcgacaa ccaaaatgac
     2221 gatatccgtg accttggctc cgcgagcccg cattgccgta aaggcttcgt ggccaggagt
     2281 gtccagaaac gtcacctgtt taccacgcac cgagacggta taggcgccga tatgttgggt
     2341 aataccaccg gcttcccctt ccgccacgtt tgtttggcga atcgcatcga ggagagacgt
     2401 cttcccgtga tcgacgtgtc ccataatggt caccaccggc ggtcgaggtt ccggccgttc
     2461 atcaccgcct gtctcaacta catcctgcag caattcgtcc ccagctttct cgaccgatat
     2521 ttcaattttg actccgcttt cctcggcgaa aattgatgcg gcatctacgt tcataggttg
     2581 gtggaacgtc aacatttgcc ccatgtccat cagttttcga acgatgtccg ccggccgttg
     2641 accgatcaac tcggcgaatt cttttaccgt cgtccgtggc gtgattttca cgctcttccg
     2701 tcggggtttg gtgatttctg ccggggtgct atggtgtaca tgcttggatc gatcatcccg
     2761 tcgctgcacc ggaatcgcac gaaggtcttg ccagcgggcc gcatcctcgc ggaatttcac
     2821 atcttgttgt tgatcatctt tagggcgacc tacttttcgt gctttcttaa gcttgtcttt
     2881 ttgtgcctcg gcctcgatgg cctcgaatgc catgcttttc tttttggccg tcacaggttc
     2941 tggcgttgtc gccccgatcg ttgacggagc aacaaccggc tttgccggca tctcttcctg
     3001 cttcgcaact agcggcacct gtgcgagtaa agaaatctcg tttggacttt cttccgttgg
     3061 aagagtgcga tcgactaggc caccactggc atgctcggtg gatggtgtct gtgtaggtac
     3121 gccgattgag gagaccggct gcgcctgacg ctcgctctca acagtcggag
//
LOCUS       DASTWE010000740         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_505023, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000740 DASTWE010000000
VERSION     DASTWE010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505023"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFT30_07650"
     CDS             <1..212
                     /locus_tag="VFT30_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092542.1"
                     /translation="AVPTSEESRLESLADPANLGGLDESDPRSVARLMKKMGEEMGED
                     VSDMEAMLDQPGGDDEGQMNHSDSF"
     gene            316..516
                     /locus_tag="VFT30_07655"
     CDS             316..516
                     /locus_tag="VFT30_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5092543.1"
                     /translation="MTSRTLKTELMTATETCRYLKITQRTLYRYLRSRQIPAFKLGKE
                     WRFVRSDLEQWIRDRTRTAVNS"
     gene            526..972
                     /locus_tag="VFT30_07660"
     CDS             526..972
                     /locus_tag="VFT30_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092544.1"
                     /translation="MAMFAGEYLCKVDEKGRFIVPSPIREQIEAEGQTVMFLKGPEQS
                     LLIYSTREWEKVLERTRTSLDEDQSRLFMHFVVSEAGSSDIDKTGRILIPGRLRKLVP
                     VDEDQEIILVGLYHRMEIWNPSEWRRYLARTEDRFEQNMAKILNLL"
BASE COUNT          396 a          427 c          386 g          371 t
ORIGIN      
        1 ttgccgttcc cacctcggaa gaatccaggc ttgaatcact cgccgaccct gccaatctgg
       61 gcggacttga tgagtctgat cctcggagtg tcgctcgcct catgaagaaa atgggtgaag
      121 agatggggga agacgtgagc gacatggaag ccatgttgga ccaaccaggt ggcgacgacg
      181 agggacaaat gaaccatagc gacagtttct gacatgtaat gtcatatact ttcacttgac
      241 attcctatca ctgtccctag aatagccgga gtattggggg tgatatgagt attcctctct
      301 cttggaggac gtggagtgac gagcaggacc ctgaagacgg agctgatgac agcgacagaa
      361 acatgtcgat atctcaagat cactcaacgt accctctatc gctatcttcg gagccgacag
      421 attcccgcct tcaagctggg aaaagaatgg cgatttgtac gttcagatct cgaacaatgg
      481 atccgcgacc gaaccagaac agccgtgaat tcctaaagac aggctatggc aatgtttgcc
      541 ggtgaatacc tttgcaaggt tgatgagaaa gggcgattca tcgtcccctc accaatccga
      601 gaacagatcg aagccgaggg gcagacggtc atgttcctga aaggtccgga acagtcgttg
      661 ttgatctatt ctacgagaga atgggagaag gtccttgaac ggaccaggac atccctggac
      721 gaagaccaga gtcgcctgtt tatgcacttt gtcgtttccg aggcaggctc atcggacata
      781 gataaaactg gacggatctt gattcctggt cgcttgagaa aactggttcc cgttgatgaa
      841 gatcaagaga tcatactcgt cggcctgtat catcgaatgg aaatttggaa tccaagtgaa
      901 tggcgtcggt atcttgcccg aacggaagat cgttttgaac agaatatggc aaagatcctg
      961 aacctcctct agttcagcta catgccatcc gaccgacgtc aaaacaattt ccgctcttca
     1021 aggtttcgcc ttcctgcaat ctcgcgccgt agtgattcta cctgctcctc cacaccgaaa
     1081 aatttatcac ggaccaatgt tacaactctg tcttcgaaat ctgatccttc gaactcaaag
     1141 gccgctcttc ggacaccatc gcatccgccg gtcatcacat cggattcatt cagagtcaca
     1201 ttgccggtcc cgcccagcat caaccatcaa tatgcgacgg tcaatggacg tcgtttgcta
     1261 tcctctgccg ggcgtgcgta taaaacatac gtcggccggc aagtctggct ggcattggcc
     1321 cagtcccctg ctcgattctc cttgcaggat cgattgcaat ccggaccact cgcgctcact
     1381 atccaattct tttttgcttc cgccttacga cgcgacctag atggcggtct gaagatcgcc
     1441 caagacgcgg tctgcgaagg gcttggcttg aacgacaatc gcatcattga aacccatctg
     1501 tataaacagg tcgacaaagc cgatccacgc attgaagtct ctctctcgtt ccttcaccgg
     1561 cctcaccact cggcctgacc
//
LOCUS       DASTWE010000741         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_407856, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000741 DASTWE010000000
VERSION     DASTWE010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407856"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="mtnP"
                     /locus_tag="VFT30_07665"
     CDS             complement(<1..628)
                     /gene="mtnP"
                     /locus_tag="VFT30_07665"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEU5092545.1"
                     /translation="MMKKKRQDGPVTVGVIGGSGLYDIEGLTSTRSIRVRTPFGAPSD
                     AITVGSLEGIRVAFLSRHGRGHLLNPSGINYRANIFALKSLGVSHVISISAVGSMKES
                     IHPGDVVVPDQFIDLTKRRASTFFDDGVVAHVAFGEPVCAELAQVLLSAGEKLGANVH
                     CSGTYLCMEGPQFSTKAESRLYRQWGVDVIGMTNMPEAKLAREAELCYA"
     gene            complement(727..>1286)
                     /locus_tag="VFT30_07670"
     CDS             complement(727..>1286)
                     /locus_tag="VFT30_07670"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEU5092546.1"
                     /translation="DPETATRLHPNDDSKVMRALEVYRLSGRPLSTAQAEHGFHETPF
                     STLLIGLERRKETLYRRIEERIDWQLTHGMVEETRLLLDQGYGRELGSMKGLGYRQVG
                     AYLANECDYAEMVRQFKRDTRRFAKRQMTWFRSEAGVTWLSIEDNEPYQRTAERAIAR
                     IEQFMRALEQQKQPAALQQGARRDE"
BASE COUNT          283 a          400 c          305 g          298 t
ORIGIN      
        1 tcgcgtaaca gagctccgcc tcacgagcca gtttcgcttc cggcatattc gtcatcccga
       61 tcacatcgac accccactgc cgatacagcc gtgattccgc tttcgtcgaa aactgcggac
      121 cctccatgca gagataggtc ccgctgcaat gcacattggc accgagtttt tctccggccg
      181 aaaggaggac ctgtgccaat tcggcacaaa ccggctcccc gaaggccaca tgcgccacca
      241 ctccgtcatc aaaaaatgtc gaggcgcgcc gcttcgtgag gtcgatgaac tggtccggca
      301 caacgacgtc gcccggatga atcgattcct tcatgctgcc taccgcgctg atcgaaatca
      361 catgagacac cccaagagat ttgagcgcaa aaatgtttgc gcggtagttg atcccgctcg
      421 gattcaacaa atgtccccgc ccatgccgtg aaagaaatgc aacccgaatt ccttcaagcg
      481 agcccacggt aatcgcatcg gaaggagcgc cgaaaggcgt acggactcgg atggaccgag
      541 tggaagtgag cccttcaatg tcatacagcc cgcttcctcc gatcactccg acggttaccg
      601 gcccatcctg tcttttcttc ttcatcatat ccttttccta acaggtgttg aaaaagtcat
      661 ccagcttcgc tctcgaaaga tacagccggc tcactatctc gccggcgcgc acaaacgtgg
      721 cgtcacttat tcgtcacgcc gtgcgccttg ctgcaatgca gcaggctgtt tctgttgttc
      781 aagagctctc ataaattgct cgatgcgtgc gatcgctcgt tccgccgtcc gctgatacgg
      841 ctcgttgtcc tcgatcgaca gccatgtaac acccgcttcg cttcggaacc aagtcatctg
      901 ccgtttcgcg aaacgtctcg tgtcgcgctt gaactgacgc accatttcgg cgtaatcgca
      961 ttcattcgcc aaataggctc cgacctggcg atagccgaga cctttcatcg aaccgagctc
     1021 gcgcccatat ccctgatcga gcaataatcg agtctcttct accattccat gagtcagctg
     1081 ccaatcgatt cgttcttcaa tccttcgata tagggtctct ttccttcgtt caagcccaat
     1141 cagaagagtg gaaaacggag tttcgtgaaa tccgtgttca gcttgcgcag tcgacaatgg
     1201 acgccccgat agccgataga cttccaatgc gcgcatgacc ttggagtcat cgttggggtg
     1261 caaccgtgtt gccgtttcag ggtcga
//
LOCUS       DASTWE010000742         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_699021, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000742 DASTWE010000000
VERSION     DASTWE010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699021"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..>1069
                     /locus_tag="VFT30_07675"
     CDS             235..>1069
                     /locus_tag="VFT30_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5092547.1"
                     /translation="MKSTVAVYAPRRLTGEALNLLAWHIPDLVAYHHHPDEWWFAPLD
                     DNLPLIRLNRIGLDMLKAMNGHTTIGTLVEQYGTKVCGPDGQPGSWHLERWATPNYSL
                     CYFGTAAPGGHRHKAKWDTLLQQVREGWSGREGFEGEEHLEEFHRHELAQSGEDDRHF
                     DLIETTVSHLFREPSEALNGLTYGRLLMRQLRRFGWFNPKPRILLEIGGGLGYLAQEL
                     GKDLLPFEKQGMTYLSLDITQPFLKLQITRAKTGGWNVSGTRANAESLPFADRSIDLV
                     ID"
BASE COUNT          237 a          326 c          278 g          228 t
ORIGIN      
        1 cacccatgcc tgttcgattc ggggacagtc gcaccgggag gacactttac tgtgcctggc
       61 ttgccagccg gccgttatcc ttaccactgc ggtatccatc ccatcatgcg aggtcagctg
      121 gtcgtgactg acgacccctc aataccatct cagttgtaaa cacccgatca tcatcttgca
      181 cgattgctga ttgctcctgt aggctgctgg aattctcatc aagagctgct cgccatgaaa
      241 tccaccgtcg ctgtctatgc ccctcgaaga ttgactggag aggcgctgaa cctccttgcc
      301 tggcacattc ccgacctcgt cgcctatcat caccacccgg acgaatggtg gtttgccccg
      361 ctcgatgaca acctgccgct catccggctg aaccgcatcg gcctggatat gctcaaggca
      421 atgaatggcc atacgactat cggtacgctc gttgaacaat atggcacaaa ggtctgtggg
      481 ccggacgggc aaccgggatc ctggcacctt gaacgatggg ctactccaaa ttattccctc
      541 tgctatttcg gaacggcagc gccaggaggc caccgacaca aagccaagtg ggatactctt
      601 ctgcagcagg tccgcgaagg gtggtcgggt cgggaagggt tcgaaggcga ggagcacctg
      661 gaagaattcc atcgtcacga acttgcccaa agcggagaag atgaccgtca ttttgatctg
      721 atcgaaacca ccgtctcaca tctcttccgt gaaccgagcg aagcgctgaa cggcttgacc
      781 tacgggcggc tcctcatgcg gcaactgcgt cgattcggct ggttcaatcc caagcccagg
      841 atcctccttg aaatcggcgg gggactgggc tacctcgcac aggagcttgg aaaagatctg
      901 ttgcctttcg aaaagcaggg aatgacatac ctgtcactcg acatcacgca accgttcctc
      961 aaactccaaa tcactcgggc caaaaccggt ggttggaatg tctcgggtac cagggccaac
     1021 gccgaatcgc tcccctttgc cgatcgttcc atcgatctcg tgatcgaca
//
LOCUS       DASTWE010000743         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1312908, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000743 DASTWE010000000
VERSION     DASTWE010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312908"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFT30_07680"
     CDS             complement(<1..395)
                     /locus_tag="VFT30_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5092548.1"
                     /translation="MLNKLNRWLQDSVFKPLEDKKMPVMEHLVEFQVRLTRAVIGLAV
                     VFTGTFFYADALVKWLRVPLQNMFVPSKLTWEPTDLPTVPFVFLAPAEALWQNVKVAG
                     LCAIVLAMPYLLFEIWRFVVPGLHAQERR"
     gene            complement(485..>1565)
                     /locus_tag="VFT30_07685"
     CDS             complement(485..>1565)
                     /locus_tag="VFT30_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5092549.1"
                     /translation="DRREFTAKVVGTDPKTDLAVIKIDAKDLPSLRWAEYEKLQVGDL
                     VLAVGSPFGLSSTVTLGIISALGRGNVGIADYEDFIQTDAAINPGNSGGALVNMNGDL
                     IGINTAIFSRTGGSEGIGFAIPSSIALDIVDSLQRTGKVVRGWMGVAIQEITPALAKS
                     FKLPEQRKGVLISDVNENGPSHAAGVKRGDVVVAFNGKEVQSVSQLRNLVARTVVGKD
                     AQVKVLREGKEQLLVVKVAERPSDEMLAKKEPAPPKEAGETMTLPDNVLASVRVQTLD
                     NALMSQLNISAKTAGVVITSVEPGGPAEAAGLQRGDVIQEVNHESIKTLGDYQKAAEK
                     IKKDELAVLLVNRQGNSLFVAINPK"
BASE COUNT          322 a          503 c          386 g          354 t
ORIGIN      
        1 aaccgtcgct cctgagcgtg aagtcccgga acgacgaacc tccagatctc aaacaagaga
       61 tagggcatgg cgagcacaat ggcgcacagt cccgccacct tgacgttctg ccagagtgct
      121 tccgccggtg caaggaatac aaacggtacc gtcggcaaat cggtgggttc ccaggtcaat
      181 ttgctcggca cgaacatatt ctggagcgga acccgcaacc acttgaccaa cgcatcggca
      241 taaaaaaatg tccccgtgaa aacgacagcc aggccgatca ccgcgcgggt gagccggacc
      301 tggaactcca caaggtgttc catgaccggc atcttcttgt cttccagcgg cttgaaaacc
      361 gagtcttgca gccaccggtt cagcttattc agcacacagc ctcgtaagtt tcggaatctg
      421 tcgatctgac gatcagtctt ccggcaatca acagattgat agatcaacag atgcccgatg
      481 cttcctactt cggattaatc gccacaaaca ggctgttccc ttgtcgattg accaacaaca
      541 cggcaagttc gtccttcttg atcttttccg cggctttttg ataatcgccg agcgtcttga
      601 tcgactcgtg attgacttct tggatcacgt caccacgttg gagcccagct gcctctgccg
      661 gtccacccgg ctcgaccgac gtgatgacca ctccggccgt cttcgctgaa atgttcagct
      721 ggctcatcag cgcattatcc agcgtttgga cacgcaccga cgcgagcaca ttgtccggaa
      781 gcgtcatcgt ctctccagct tccttcggcg gcgccggttc tttcttggcc agcatttcat
      841 ccgaggggcg ttcggccacc ttcacgacga ggagctgttc cttgccttcg cgcaggacct
      901 tgacctgcgc gtccttccct accaccgttc tggcgacgag attgcgcagt tggctgacgc
      961 tttgaacctc cttgccgtta aatgccacga cgacatcgcc tcgcttcact ccagcggcat
     1021 gggaagggcc gttttcattg acatcgctga tcaacacacc cttccgctgc tccggaagct
     1081 tgaacgattt cgccaaggcc ggcgtaattt cctggatcgc gacgcccatc catccgcgta
     1141 cgaccttgcc cgttctctgc agactgtcca cgatatcgag ggcgatgctg ctgggaatcg
     1201 caaacccgat cccttccgat ccaccggttc gcgagaagat cgcggtgttg ataccaatca
     1261 gatctccgtt catgttgacg agcgctccgc ccgaatttcc gggattgatc gccgcgtcgg
     1321 tctgaatgaa atcttcatag tcggctattc cgacattccc acgtccaagg gcgctgatga
     1381 tgccgagcgt gacggtcgaa cttaggccga acgggcttcc gaccgccagc acaagatcac
     1441 ccacctgtaa cttttcgtac tctgcccacc tcagcgacgg cagatccttg gcgtcgatct
     1501 tgatgaccgc caaatccgtc ttcggatctg tgcccaccac ctttgccgta aactcccgcc
     1561 gatcg
//
LOCUS       DASTWE010000744         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_925196, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000744 DASTWE010000000
VERSION     DASTWE010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925196"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFT30_07690"
     CDS             <1..317
                     /locus_tag="VFT30_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1348 family protein"
                     /protein_id="HEU5092550.1"
                     /translation="SGRESIVKFLRRKWEFELDYRLIKELWVYQHNRIAVRFAYEWHD
                     NSGNWFRSYVNENWEFDGDGLMRLRIASINDLPIKESERKYRWALGKRPDDYASLSDL
                     SL"
     gene            complement(449..1441)
                     /locus_tag="VFT30_07695"
     CDS             complement(449..1441)
                     /locus_tag="VFT30_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5092551.1"
                     /translation="MMRTFTTTVVTTSLVALTGCSSLKQPETYIRQPTVCIDKNWYGY
                     HKAGGCPSTTKTVAPDASKETAARLAALEGDRQRLADELEAARRQNDALSSRVSDLER
                     QLADHDSQLSEARTERDQVAAKLATLQSSAGEADTLSSELAAARQHIEDHDRLAAELE
                     AQLAAARQRIADLEGQLGESKGSLAEAERDLLKALRPEISKGTVSVNQAGDALTINLA
                     SSLLFDSGQEKLKAGGADALRRVGAVLKEFPEKQVHVSGYTDNVAIKGALKKKFPSNK
                     ELSDARADSAAEALRDGGVTTNLSAAGHGETNPVATNNTAEGRAKNRRVEVIVQ"
     gene            complement(1511..1660)
                     /locus_tag="VFT30_07700"
     CDS             complement(1511..1660)
                     /locus_tag="VFT30_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEU5092552.1"
                     /translation="MIAFVIFMLVKQVNRFKKEAPPPSPSAPPPPTNEEKLLMEIRDL
                     LKNRQ"
BASE COUNT          338 a          466 c          431 g          425 t
ORIGIN      
        1 tgtcaggacg agaatctatc gtcaaatttc tgcgccgcaa atgggaattc gagctggact
       61 accgcttaat caaagaactg tgggtgtacc aacataatcg aatcgccgtg cgcttcgcct
      121 acgagtggca cgacaattct ggaaattggt tccgatccta tgtcaacgaa aactgggagt
      181 tcgatggaga cggccttatg cgtctacgaa tcgccagcat caatgacctt cctatcaagg
      241 aatcggaacg aaagtaccgc tgggctctgg gcaaacgacc tgatgattat gcctcccttt
      301 ctgacctcag tctctgatat tgattgggaa gccaggtgag tgcggcacgc ctttaagatg
      361 gtgtgcattg ggattgatgt agcgacacaa tggaagaggc tcttccatag ggagagcctc
      421 ttccattgat caacaaaagc tggccttgtt attggacgat gacctcgacc cggcgattct
      481 tggctcgccc ctccgctgtg ttattagtcg caacaggatt tgtctctcca tgaccggcag
      541 ccgacagatt ggtggtcacg ccaccgtccc gcaacgcctc agcagcgctg tcggcccggg
      601 cgtccgagag ttccttattg gacgggaact tcttcttcaa cgcacctttg atcgccacat
      661 tatcggtata ccctgagaca tggacctgtt tttccgggaa ctctttgagg acggcgccta
      721 cccgtcgcaa ggcatcggcc ccccctgcct tcagcttttc ttgacctgag tcgaacaaca
      781 gacttgaggc aagattgatc gtgagcgcat cgccggcctg gtttaccgac acggtcccct
      841 ttgagatttc aggtcgcagt gccttgagga ggtccctttc ggcctctgcc agactgccct
      901 tgctctcacc caactgccct tcaagatctg caatccgttg cctcgccgcc gctaattgtg
      961 cctccaactc cgctgctagc cggtcatgat cctcaatatg ttgcctcgcg gcagctaatt
     1021 cggaggacag tgtatccgcc tcaccggcac tactttgtaa ggtggctagc tttgctgcga
     1081 cctggtctcg ttcggtcctg gcctctgaga gctggctatc gtgatcggcg agttggcgtt
     1141 ccaagtcgct gacgcgactg ctgagggcat cattttgtct ccgtgccgct tccagttcgt
     1201 cggccagtcg ttgacggtcc ccctcaagag ccgcaagacg ggcggccgtt tctttcgatg
     1261 catctggtgc cactgtcttg gtcgtggagg ggcacccgcc ggctttgtgg tagccatacc
     1321 aatttttatc aatacagaca gtcggctgtc taatataagt ctctggctgc ttgagagacg
     1381 aacaacctgt gagagcgact aagcttgtcg taaccacagt tgtagtgaac gttcgcatca
     1441 tgatgatccc ctccttctcc gtcgcgcagc tctggcctcg caacgcttcg attctgggta
     1501 tagcggactc ctactgacga ttcttgagca gatcgcggat ctccatcaac aatttttcct
     1561 cattcgtcgg cggtggtggg gcggaaggtg acggtggagg tgcttctttc ttaaaccgat
     1621 tcacttgttt aaccaacatg aaaataacga atgcaataat
//
LOCUS       DASTWE010000745         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1054658, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000745 DASTWE010000000
VERSION     DASTWE010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054658"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFT30_07705"
     CDS             <1..759
                     /locus_tag="VFT30_07705"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5092553.1"
                     /translation="LSESERLEGAEALGRIAKTLRPALVLGVQGPNTETAIRYAKHAK
                     KVGADAVISLPPAGEKDPKAIADFYRQVGNATDLPLFVQAVGNMTVPMLVDMYKAIPT
                     LRYVKDEAGEPLLRVGELRRQTSDQLRVFTGMHGRTMIDEMRRGFSGTMPAASFADLY
                     ASVWNSWHAGKQAEAIEVFGRTSMLIAEVSVYGIESLKYILHLRGVFKTYKVRSVPNN
                     SPSPVSVGLDGRMDEGARQVIREMLDYLKPWLKA"
     gene            809..>1013
                     /locus_tag="VFT30_07710"
     CDS             809..>1013
                     /locus_tag="VFT30_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092554.1"
                     /translation="MLQIFGVKNSQSTRAAERFFKERRVKVQSVDLMKKPMAPAEISR
                     FIVRFGLTGLLDVEGRAYIDGGLK"
BASE COUNT          240 a          242 c          311 g          220 t
ORIGIN      
        1 ctgagcgaat cggagcgtct ggaaggtgct gaagcgctcg ggcgcatcgc gaagactctc
       61 cgtcccgcac tggttctcgg tgtacaaggg ccgaatacgg aaacggctat ccgctacgcc
      121 aaacacgcga agaaagttgg cgcagacgct gtgatttctc ttcctcccgc cggggagaag
      181 gatcctaagg caattgccga cttctatcga caagtaggaa atgctacgga tctgcccctg
      241 tttgtgcaag ctgtcgggaa tatgacggtc cctatgctag ttgacatgta caaggccatt
      301 ccaacactgc ggtacgtgaa ggatgaagct ggggagccac ttctgcgggt tggtgagctg
      361 cgaaggcaga cgtcggatca actgagagtg ttcaccggca tgcatggccg cacgatgatc
      421 gacgagatgc ggcgtggctt cagcggcaca atgcccgcgg cttcctttgc agatctctat
      481 gcgtcggtct ggaattcctg gcatgcaggg aaacaagcag aggccataga agtgttcggt
      541 cgcacttcga tgctgatcgc ggaggtgagc gtgtatggca ttgaatcgct aaagtacatc
      601 ctgcatcttc ggggcgtgtt caagacttac aaagtgcgat cggtaccaaa caactcacca
      661 tctccggtat cggtcggtct cgacgggcgg atggatgaag gtgcgagaca ggtgattcgc
      721 gagatgctcg actatctcaa accgtggttg aaggcttgaa cgcggcacta agttcataga
      781 taagagaact gcgggactag agagctatat gttgcaaatc ttcggagtaa aaaattctca
      841 atcgacccga gctgccgaac gattcttcaa ggagcgtcga gtaaaggtgc aatcggtgga
      901 cttgatgaag aagccaatgg ctccggctga gataagccga ttcattgtcc gttttggcct
      961 aaccgggttg ctggacgtcg agggccgggc gtacattgac ggagggttga agt
//
LOCUS       DASTWE010000746         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1086866, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000746 DASTWE010000000
VERSION     DASTWE010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086866"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1458)
                     /locus_tag="VFT30_07715"
     CDS             complement(67..1458)
                     /locus_tag="VFT30_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5092555.1"
                     /translation="MSASILVVDDEEAIRTSLRSILEDEGYDVSVAANGVEALKIYGI
                     DPPDLMILDIWMPEMDGLETLRRVKEFVPMTQVMMISGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALEQFRLAQENRSLRTKVQQKFELVGQSPAMQRLRELIETAGPT
                     NSRVLIGGENGTGKELVARAIHMHSTRSDHPFVAVNCAAIPETLIESELFGHEKGSFT
                     GATSMKRGQFEQADGGTLFLDEIGDMSLNTQAKVLRALQEQQFTRVGGTKLMKVDVRV
                     LAASNKDLEKEIGKGQFRDDLYYRLNVVPIVVPPLRERREDIPALVQHFMKMHVEEQG
                     LRMKEVSSEAMTVFQHYEWPGNIRELRNLIERLMIMVPGFVIDASQAALSLQGRTAGV
                     APTGNQAANPLLTKFYDSLRDARNAFEKEYISRKLREHHWNISRTAEDLKIERSHLHR
                     KIKLLDVEMRPES"
BASE COUNT          313 a          480 c          366 g          388 t
ORIGIN      
        1 ccaacacgaa gttccaggat ctcgtctgct ccacgtgcac aggacactaa tcctgtcatt
       61 gtcttctcag ctctccggcc gcatctccac atcgagcaac ttgatcttgc ggtgaagatg
      121 actccgctcg atcttgagat cttcggcggt tcgtgaaatg ttccagtggt gctcccgaag
      181 cttgcgactg atgtattctt tttcgaacgc attcctggca tctcgaagcg aatcgtagaa
      241 tttcgtgagg agtgggttgg ccgcctgatt gcctgtcggc gccacgccgg cggttcgtcc
      301 ctgtaatgac agagcggctt gggaggcatc gatcacaaac cccggcacca tgatcatgag
      361 ccgctcgatc aaatttctca gctcccgaat gttcccgggc cattcgtagt gctgaaacac
      421 ggtcattgct tcggacgaaa cttccttcat ccgcaaccct tgctcttcga catgcatctt
      481 catgaaatgt tgcacaaggg ccgggatatc ttctcttctc tctcgcagtg ggggcacaac
      541 aatcggaacc acgttgaggc ggtagtacag atcgtcccga aattgcccct tgccgatttc
      601 tttttccaaa tccttgttgg aagccgccag cacccgcaca tcaaccttca tcagtttggt
      661 ccccccgact cgcgtgaact gctgttcctg caaagcccgt aacacttttg cctgcgtatt
      721 gaggctcatg tctccgattt cgtcaagaaa cagcgtaccc ccatcggcct gctcgaattg
      781 gccgcgcttc atggatgtgg ctccggtgaa ggagcccttt tcgtgaccga acaattcact
      841 ctcaatcaat gtctcaggga tggctgcaca attgacggcc acgaacggat ggtccgatct
      901 ggtgctgtgc atatggatgg ctcgagcgac caactccttc cccgtaccat tctcgcctcc
      961 gatcaacacc cggctgttcg tggggcctgc cgtttcgatg agctcccgta gccgctgcat
     1021 ggccggagac tgcccgacca actcgaactt ttgctggacc ttggtccgca gcgaccggtt
     1081 ctcttgcgcc aatcggaact gttccaacgc gtgtttgaca cgaagcgtga cattttcgag
     1141 cgacaacggc ttctcgatgt agtcgtacgc acccaacttg atagctttca cggccgtttc
     1201 gatggagcca tgcccggaga tcatcatgac ctgcgtcatg ggcacaaatt ctttcacccg
     1261 tcgtaaggtc tccagcccgt ccatctccgg catccagatg tccagaatca tgagatcggg
     1321 cgggtcgatt ccatatattt tcagcgcctc caccccattg gcggctacag acacgtcata
     1381 tccttcatct tcaaggatgc ttcgcaagga cgtgcgaatc gcttcttcgt cgtccaccac
     1441 tagaatcgac gccgacataa cccttttctc cttaccactt acccatcatc ggttacctac
     1501 accggcaaat cgaacgtaaa cacggctccc ttcgggtgat tgttccc
//
LOCUS       DASTWE010000747         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_634338, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000747 DASTWE010000000
VERSION     DASTWE010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634338"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFT30_07720"
     CDS             complement(<1..351)
                     /locus_tag="VFT30_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEU5092556.1"
                     /translation="MDTAVLVLITFVAATVNGALGYGFSSITVPVALLFYTNRILNPA
                     LVLIELVINGYVLFMNRNSIPNVWRRVAPILIGLVIGIGIGSYILFLVQPAWIKFVTY
                     FFLLPLILLQAAGIR"
     gene            complement(358..792)
                     /locus_tag="VFT30_07725"
     CDS             complement(358..792)
                     /locus_tag="VFT30_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5092557.1"
                     /translation="MKLSKKSEYGLRALLELTLVHGKTTLQRHDIADRQHIPIEFLEQ
                     ILLTLKRAGFISSRRGVNGGYVLIKQPNEITLGQVIRILDGPLAPIGCVSKTAYQKCS
                     DCPYANKTRCPLQQVMGTVRDAIAGILDHYSLADFAAGHPKD"
BASE COUNT          271 a          234 c          287 g          242 t
ORIGIN      
        1 tcggatgccg gcagcttgaa ggaggatcaa cggtaacaaa aagaaatagg tgacgaattt
       61 aatccatgca ggctgaacca aaaagagaat gtagctgcca atgccgattc caatgaccag
      121 cccgattaga ataggggcta cacgccgcca gacattcggg atactgttcc gattcatgaa
      181 cagtacatac ccattgatga cgagttcgat cagcacgagg gcagggttca agatgcgatt
      241 ggtgtagaac agcaaggcga cgggaacggt aatcgaggag aagccgtatc ctaaggcgcc
      301 gttcaccgtc gcagcgacaa acgtaatgag gacaagaacg gctgtatcca tgagtgctta
      361 gtctttggga tgacctgcgg caaagtcggc aagcgaatag tgatcgagga ttccagcaat
      421 cgcatcacgg acggtgccca tgacttgttg taggggacac cgcgtcttat tcgcataagg
      481 acaatcgctg catttctggt acgccgtctt actgacgcag ccgattggag ccagcggacc
      541 atcgagtatg cgaataactt gaccgagtgt gatctcgttc ggctgtttaa taaggacata
      601 tcctccgttc actccacgtc ggctggagat gaagccggct cgtttgagcg tgagaagaat
      661 ctgctctaag aattcaatgg ggatgtgctg gcgatcggca atgtcgtgac gctgcagtgt
      721 cgtctttccg tggaccagcg tgagctcgag caatgcccgg aggccatatt cacttttctt
      781 tgaaagtttc ataagtagag tgagtttatc aacaatagtt aggataaacc gtgcttgttc
      841 gtcaaggtca aagtttctga gcgaatcggc agtgggaatg tcgccattgc acagggcgag
      901 gtcgctacct cacgtggaat caattgtcac tggatatggt cctcctaatt cttcaaattc
      961 cagaagccaa actgagttcg tcttcattga aagtttgcac tcgtgaaggc agggctgctg
     1021 atatggtcgt gacc
//
LOCUS       DASTWE010000748         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_570113, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000748 DASTWE010000000
VERSION     DASTWE010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570113"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFT30_07730"
     CDS             <1..997
                     /locus_tag="VFT30_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092558.1"
                     /translation="IKQVLTFARGAVGERVPTQIRYLLKDLINVLKDLLPKNVTLKFD
                     VQQDLWPVMADPNQIYQALMNLCINARDAMSLGGVVTLKAHNIEIDENYALVNPIAEP
                     GKYIVITVEDTGSGIPREVIDRIFDPFFTTKEVGKGTGLGLSTAQTIVAGHKGFISVY
                     SELSKGARFSVYLPAVPEQAEEKKTYEPDLPRGHGELILLVDDEMDMRTIAKATLEKF
                     GYRVITAVDGTDAVATFTLNKEEVALVLTDMAMPFLDGIGTIRAIQHISPNARFVAMS
                     GMMGTAANSELESLKVSGLLIKPFTTATLLSTLAKALSDEPNPTGLHEDIRGRNL"
BASE COUNT          267 a          260 c          291 g          243 t
ORIGIN      
        1 gatcaagcaa gttctaacgt ttgcccgcgg ggcggtgggt gagagggtgc cgactcagat
       61 caggtattta ctaaaagact tgatcaacgt acttaaggac ctgcttccca agaatgtcac
      121 gttgaagttt gatgttcagc aagatctctg gcccgttatg gcggatccga atcagatata
      181 tcaggctcta atgaacctct gtatcaatgc tcgagatgca atgtcgttag gcggcgtcgt
      241 aacgctgaaa gcgcacaaca tagagatcga tgagaactat gctctcgtaa acccgatagc
      301 ggaaccgggt aaatatatcg ttatcacggt agaggacacc ggcagcggta tcccgcgaga
      361 agtgatagat aggatctttg acccattctt tacgacgaag gaggttggca agggtaccgg
      421 tctgggactc tcgacagcgc agaccatcgt tgccggccat aaggggttca ttagtgtcta
      481 cagtgagctg agcaaggggg cacggttctc ggtttacctt ccggccgtac ccgaacaggc
      541 tgaagaaaaa aagacgtatg agcccgatct tcccaggggg cacggcgaat tgatcctgtt
      601 agtcgatgac gaaatggata tgcgcacgat cgcgaaggcg acgcttgaga aattcggtta
      661 ccgtgtgatc accgctgtcg acgggacgga tgcggttgct acgttcacct tgaataaaga
      721 ggaagtcgct ctcgttctga ctgatatggc gatgccgttt ctcgatggta tcggaacgat
      781 ccgggcgatc caacacattt ctccgaacgc cagattcgtt gcaatgagtg ggatgatggg
      841 aacagccgcc aattccgaac tcgagtcttt gaaggtaagc ggtctgctaa taaagccatt
      901 cacaaccgcg acactgctct ccaccctggc gaaggcttta tcggatgaac ctaatccaac
      961 gggattacac gaagatattc gcggacggaa cctgtgacgt ctcccacacc gtcgcattgt
     1021 ccgtccgctt atttgaccgt ttgtgccggt caaacctcca a
//
LOCUS       DASTWE010000749         1843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_634371, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000749 DASTWE010000000
VERSION     DASTWE010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634371"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..397)
                     /locus_tag="VFT30_07735"
     CDS             complement(77..397)
                     /locus_tag="VFT30_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092559.1"
                     /translation="MKEVTKGLTVSTSVIPAPVRRGPKRLSPNHLLELVTRLCIFKAE
                     RWGRRALFYERHRDYFPRLSVGRFVSRCRELEARYRALAQVMFLIPAEDRLIPIRALA
                     LPKL"
     gene            complement(616..945)
                     /locus_tag="VFT30_07740"
     CDS             complement(616..945)
                     /locus_tag="VFT30_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092560.1"
                     /translation="MTKVTNLSEESCRTSFTHQLSAILAHEGETPAAANALAGKTVST
                     LITYDLGPRPFAIAAPSGTDYRFFIDRKGTNCVLTLFGRRKGFVSYTNNLTYIATEIL
                     PDCTCAD"
     gene            complement(1095..>1843)
                     /locus_tag="VFT30_07745"
     CDS             complement(1095..>1843)
                     /locus_tag="VFT30_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092561.1"
                     /translation="TRAGARRLIEQTPKALAGLAAISGVAYLAGSIYTKAYFSEFGAS
                     WILEEVPAATYFSQSWVPLLLMLYFGYLATTNLALIGSQNDVTASFQFRISVALVRYG
                     SWFLFALLVITPLLSTFGYVMWAIVLSIIGVTAILLLFSSALELVVVRLNTKDRFIDL
                     SMAYIAFAVIAAGLYVVPVQLGLNWARIDKQPTSSLLTVYLHSDAEKEYRLLFSVGER
                     LYVFPARFEEAYPPVQATAIANVSFVPIEP"
BASE COUNT          479 a          487 c          488 g          389 t
ORIGIN      
        1 cccatataca gtcaacatct gtacacgtca tctactgtgc cgagacacag ttggacagag
       61 agacctgctg ctgctcttat aacttcggca gggccaatgc acgtatcgga attaaccgat
      121 cctctgccgg aataagaaac atgacctgtg cgagcgctcg atacctcgct tccaactcac
      181 ggcatcgact cacgaatcga ccaacggaca accggggaaa ataatcgcga tgccgttcat
      241 aaaagagcgc ccgccgcccc caacgttccg ccttgaagat gcacaaccgg gtgaccagtt
      301 ccaaaagatg gttcggtgac aaccgcttgg gtcctcgtct caccggagcg ggaatcacag
      361 aggtggatac ggtcagtccc tttgtcactt ccttcatagt gtcctccttg tgcctgaacc
      421 atcatctcga gcatcgtgac tcgagtcgct tgtgcaccca taatcccggt tcatcttggc
      481 attatgttat agttccggaa catgaagaga tcattaggag aagatgaaaa cttcttcata
      541 aatgctagaa attcatggat cacctgcgat ctgacagagt agaaggggaa aatgtaggta
      601 agacgtttgg gtcggctagt cggcacaggt gcagtccggg aggatctcgg ttgcaatgta
      661 ggtcaagttg ttggtgtagg agacaaaccc tttccggcgt ccaaagaggg tcagcacaca
      721 gtttgttccc ttacggtcga taaaaaaccg gtaatcggtg ccggatgggg ccgcaatggc
      781 gaaggggcgt ggtccaagat cataggtgat gagagtcgag acagtcttgc ctgcaagagc
      841 attcgcagcc gctggcgttt caccttcgtg agcgagaatc gccgatagtt gatgggtaaa
      901 cgaagtgcgg caggattcct ccgagagatt cgttacctta gtcaccgtgg cgcaactcgc
      961 cgtcataagg acaatcgccg ggaataaagc ccaggaccgg tggatggcgt accctgatcg
     1021 cgtcatatga atgcagactg gctgtggagc ggaagtgtag cctctacccg tcccctcgca
     1081 caacatgttc tttcttacgg ctcaatgggt acgaagctga cgttggcgat ggcagtagct
     1141 tgaactggag gataggcctc ctcgaatctt gctggaaaca catacaagcg ctccccaacc
     1201 gagaacagca gccgatattc cttttcggca tcactgtgca gatacacggt gagcagcgag
     1261 gatgttggtt gtttatcgat ccgtgcccaa ttcaggccga gctgcacagg gaccacatac
     1321 agaccggcgg caatcacggc gaatgcgata tacgccatcg acagatcaat gaaccgatct
     1381 tttgtattga gccgtaccac aaccagctcg agcgctgaac tgaacaagag cagaatcgcg
     1441 gtaacaccga taatagacag aacaatggcc cacatgacat agccgaatgt gctcaagagc
     1501 ggagttatca ctaagagcgc gaacaagaac catgagccat accgaaccaa ggctacggag
     1561 atcctgaact gaaagctcgc agttacgtcg ttttgactcc cgatcagcgc gaggttcgta
     1621 gtagctagat acccgaagta gagcatcagt aacagcggca cccagctctg actaaaatag
     1681 gtcgccgccg ggacctcctc taagatccac gaggctccaa attcagagaa atacgctttc
     1741 gtgtaaatcg atccggcgag atacgccact ccactaatgg ctgctaatcc ggccaatgcc
     1801 ttcggcgtct gttcgatgag tcgtcgagcc ccggctcggg tag
//
LOCUS       DASTWE010000750         1690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_505340, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000750 DASTWE010000000
VERSION     DASTWE010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505340"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..857
                     /locus_tag="VFT30_07750"
     CDS             213..857
                     /locus_tag="VFT30_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092562.1"
                     /translation="MPLFSRFVSHQRLGVRGALLCLAASAVFPPHVLAEALDHLSDLT
                     DKAAQAVVTLKGRDNFNSEYLYDVSVKNLSSDPLVGESLIIILDKITNIGGDEWMAGT
                     SESYLRRMEILGQDGETDDGKPYFRIPRGPGPDLPPYTESSPATVRIRNKDYLIVFTP
                     FFRVYGMKRPSVSLKPKERVSPLPPAKTPADKLIQMLIQKGVLSEEEGRELYQH"
     gene            1236..1592
                     /locus_tag="VFT30_07755"
     CDS             1236..1592
                     /locus_tag="VFT30_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092563.1"
                     /translation="MKALKSSSLVLAIVTVGLLAVLGTRAEAQSPSKTSSSMIVRGEV
                     SQVEGEFHMAKDSQGLDTLDIVDKSYVITDRAGKELRLELSDDTKVLNRVNPGDKIEA
                     KISIEGHTLSVIRLKP"
BASE COUNT          411 a          419 c          456 g          404 t
ORIGIN      
        1 atcctgtttg agcgttcgtg aagtcgaaga actcttagct gaaacctgga gtcacggtgg
       61 cgtatgaaac cgttcgccag tggtgtcgga agtttgggtc agcctatgtg cataagggat
      121 ggtgtcactg ttcgatccat ccgttccatt gtgcgtgcag ccggttaagt tgacaatacc
      181 ataatttttg agcgatcaat ccggagcgtg ccatgcctct tttttctcgt tttgtttccc
      241 atcagaggct cggagtacgg ggcgcgcttc tgtgcctggc cgcgagtgcg gtctttcctc
      301 cacatgtcct ggccgaggca ttggaccacc tttctgactt gaccgacaaa gcggctcagg
      361 ccgtcgtcac tctcaagggc cgtgacaact tcaacagcga gtatctgtac gatgtgagtg
      421 tgaagaactt gtcgtccgat cccttggtgg gagagtcgct catcatcatt cttgacaaga
      481 tcaccaatat cggtggcgac gaatggatgg cgggcacgag cgaatcatat ctgagacgta
      541 tggagatcct ggggcaggat ggcgaaacag acgacggcaa accttacttt cggatccctc
      601 gcggacccgg tccagatctc ccaccctaca ccgaaagcag tcctgctacc gtgcggattc
      661 gcaacaagga ctatctcatc gtgttcactc cgttttttcg ggtgtatgga atgaaacggc
      721 cgtccgtttc gctgaaaccg aaggagcggg tgtcgccatt gcctccagcc aagacccccg
      781 ccgacaagct cattcagatg ctcatccaga aaggcgtgct ctctgaagaa gaagggcgcg
      841 agctctatca gcattaatct gtcgggcagc gagtcacaaa actgattcgt cctgacaatt
      901 aaataaactg atcaattgtg agcagattgc gagtcgcgcg cagtgcagaa tagattccag
      961 acccaattca gacgtatacc gacagagaag ttcaggcaga gagattgggc caatcctcaa
     1021 ggctggccta atctatccat acctagaagc tagaaggata tggaaaatca tttcggcaca
     1081 gtagaatcga tgcctacaag tctggaacga gaaggagacc tcccacggga tgcttcacgt
     1141 gcgttaaccc gattggcttc cgggtgatca ggataggaat ccgggggtga tcaagatagg
     1201 agaggctttt cagcctcagt cagatggagg tccttatgaa ggccctaaag tcgtccagtc
     1261 ttgtcttggc catagtcact gtcggtctct tagccgttct gggaactcgc gcggaagccc
     1321 agtcgccttc caaaacatca tcttccatga tcgtgagagg cgaagtgtct caggtcgaag
     1381 gagaatttca tatggccaag gattcccaag gtttggacac gctcgacatc gtggacaaat
     1441 cttatgtcat tacagatcga gccggcaaag agttgcgtct ggaacttagc gatgacacca
     1501 aggtactgaa tagagtcaat ccaggggaca agatcgaagc caagatttca atcgaaggtc
     1561 atacgctctc ggtcatacgg cttaagccat aacgcactgt cattgttttc aaaagggagg
     1621 tagccatgtt gatgttcaaa ttgttcatcg tgtgtggagt gattgccgtc ggatccctgt
     1681 ctgtcgaggc
//
LOCUS       DASTWE010000751         4414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_408220, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000751 DASTWE010000000
VERSION     DASTWE010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408220"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFT30_07760"
     CDS             <1..812
                     /locus_tag="VFT30_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5092564.1"
                     /translation="IPARETSWVDSLTRAVQARQVSAIRALAFVGESTIAFGRALLRP
                     RSIRWRALLRIVELDGVRALPITGLLTFLVGVVIAYQGAEQLRKFGTNIFIVDLVGIS
                     LLREIAPLIVAILIAGRSGSAYAAEIGTMKVTEELDAVRTLGISPMNLLVLPRALALV
                     IALPLLTVYADVVGVFGGMLIALGELNVSFVEFVARFDEAVPVRHFLIGLGKAPFFAA
                     IIALVGCYQGFQIRGGVDDVGRHTTVSVVQGIFLVIVFDAICSILLNWWDL"
     gene            904..1713
                     /locus_tag="VFT30_07765"
     CDS             904..1713
                     /locus_tag="VFT30_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5092565.1"
                     /translation="MPLAVQSEPSIIEASHVETRFGSAVVHEDVSLSIRRGEIFAIAG
                     GNGSGKTTLLREIIGLITPSAGTIRLFGIDSRRLEAGDGHPIHRRFGVMFQHGALFSS
                     FTLAENVAVPLREHTTLSAGLIRDIVAAKIAMVGLPPDSAAKYPNELSGGMRRRAALA
                     RAIVMDPELLFLDEPTAGLDPIIAAGFDDLVLSLKSLLGLTVVMVTHDLDSLWRIANR
                     VAVLGRGKVLGIGTMQELSRSDDPVIREYFQGPRGRAASEQAAWNHERREI"
     gene            1830..2792
                     /locus_tag="VFT30_07770"
     CDS             1830..2792
                     /locus_tag="VFT30_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5092566.1"
                     /translation="MEPKVNYILVGSFVAFLGAAVLVGILWLGKTDYRGSYDRYEAYM
                     RESVAGLSVDSTVKYRGVDVGRVKAIALNPTNPEEVLLTMDIMRGTPIKTDTIAVLET
                     QGLTGLATINLTGGSRDAPSLQAQEGQAYPVIKTGPSLFFRLDEAVSRLISEEGLAQL
                     LVDLDTAAKGAAKVLDEDNRTMLKRTIKDLSDVAQTIAAHKTQIEQSLSGAAKSADNL
                     VKLTASLNAEVPTLLAGINKSVAALGTATDELARTSKTVGAVINEARPELQQFTRRTL
                     PEASLLVTELRQLTGTLTRVARELEREPSSLVFGRKSPSRGPGE"
     gene            2817..3491
                     /locus_tag="VFT30_07775"
     CDS             2817..3491
                     /locus_tag="VFT30_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEU5092567.1"
                     /translation="MMNYRLAHVVCVLLALTVAGCLSPRMDSSEIHTYQLSLDGWPSE
                     SRPGKLDGPVLLVSPPQAEPGFETQRMVYVKRPYELEYYALNQWADTPVRMFAPLMIQ
                     ALNQNDVWRAVIPLPSSIHGDYRLDTHGFLLQQEFLQQPSRVRVMVRTQLVDLKESTI
                     LNTRTFEVVENATSENPYGGVQASNRAIAGLLDQIGSWLRQCVQHSPECLADGAGSGF
                     IGKNKP"
     gene            complement(3602..>4414)
                     /locus_tag="VFT30_07780"
     CDS             complement(3602..>4414)
                     /locus_tag="VFT30_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEU5092568.1"
                     /translation="RHFLYEPMRCRHQGSNVLISFLSIERLTDISQLDHIHLEPNAVH
                     LWGIELNGSRRCLERCTGWLDDVERDRAARLVREDTRQRYVLAHGGLRAMLSRYLGVN
                     PDAVPLGRSAIGKPFLKRELRDRPAITFNLSHAHDRALIAVSKVQEVGVDLEFVRADI
                     EVANLSKRFFTSSEHTAIMQVTEERRATRFFRYWVAKEALLKAQGIGLGGLPDCEIFF
                     ETDGVGTDVRTRLGTQFPHTLRVRLLPCEKGWEAAVAAQQLDAVKLCGFEHL"
BASE COUNT          943 a         1161 c         1307 g         1003 t
ORIGIN      
        1 ggatcccggc tcgcgagacg agctgggtgg acagtctcac gcgtgccgtg caggctcggc
       61 aagtatccgc gattcgcgcg ctcgcattcg tcggagaaag caccatcgcg ttcggccgag
      121 cgctattacg gccacgttcc atcagatggc gggcgctctt gcggatcgtg gaactggacg
      181 gcgtgcgggc cttgccgatc accggcctgc tgacatttct ggtcggtgtc gtgattgcct
      241 atcagggcgc ggagcagctc cgtaagttcg gcacgaacat ttttatcgtg gatctggtcg
      301 gcatctcgtt gttgcgcgaa attgccccgt tgatcgtggc aatcctcatt gccggccgtt
      361 cgggatccgc ttatgctgct gagatcggca ccatgaaggt gacggaagaa ttggacgcag
      421 tgcggacatt gggcatctca cccatgaatc tgctcgtgtt gcccagagcg ctggccctgg
      481 tcattgccct ccccttgtta actgtgtacg cggacgtggt cggcgtgttc ggcggcatgc
      541 tgattgccct aggagaactc aacgtgagtt tcgtggagtt tgtcgctcga ttcgacgaag
      601 ccgttccggt ccggcatttc ctcatcggtt tagggaaggc gccattcttc gcggcgatca
      661 tcgctttggt gggctgttat caaggattcc aaattcgagg cggcgtggac gacgtgggtc
      721 gacacaccac cgtcagtgtc gtgcagggta tttttctagt gatcgtattt gacgcgatct
      781 gtagcattct gcttaattgg tgggatttat aaagaagaag gttgaggttc aggttgaggc
      841 taagagagct gccccttaat ctaaacctta gcctcacctt ctcggttagg agggcgattc
      901 ctgatgccgc ttgcagtaca aagtgaacct tcgatcatcg aagcgagcca tgtggagaca
      961 cgattcggat cggctgtcgt gcatgaggac gtcagtctgt ctattcggcg gggtgaaatc
     1021 ttcgccattg cgggcggtaa cgggtccggc aagacaacgt tgttgaggga aatcatcggc
     1081 ctgatcacac cctcggcggg aacgatacgg ctgttcggaa tagacagccg gcgattggaa
     1141 gcgggtgacg gccatcccat ccaccgccgg ttcggtgtga tgttccagca cggtgcatta
     1201 tttagttcgt tcacgctggc ggaaaatgtc gcggtgccgt tgcgcgagca tacgacgctg
     1261 agtgccggat tgatccgaga catcgtcgca gccaaaattg cgatggtggg actaccgccg
     1321 gacagtgccg cgaagtatcc caacgaactc agcggaggga tgcggagacg ggccgcactc
     1381 gcccgagcga tcgtcatgga cccggagttg ttgtttctcg acgaaccgac ggccggactc
     1441 gatccgatca tcgctgcagg cttcgatgat ctcgtcctct cgttgaagag tctcctagga
     1501 ctgacggtgg tgatggtgac acacgaccta gattcactgt ggcggatcgc taatcgtgtg
     1561 gcagtgctcg gtcgtggaaa agtgttgggc atcggcacta tgcaggagtt gtctcgatcg
     1621 gacgatcctg tcatacgtga atacttccaa ggtccacgcg ggagggcggc aagcgaacag
     1681 gcggcatgga accacgaaag acgtgaaata tgaataggag caaatggctc atggctgata
     1741 gcacgaacga ggaaactcat tgtttggatt cgcatttttt ctataagtca tacgctataa
     1801 gctattcgct cttctgaacg agattaaaca tggaacccaa ggtcaactat attctggtcg
     1861 gctcgttcgt ggcatttctt ggcgcggctg tcctggttgg aattttgtgg ctcgggaaga
     1921 ccgactaccg cggttcttat gaccggtacg aggcctatat gcgggagtcg gttgcaggat
     1981 tgagcgtcga ttccactgtc aaatatcgcg gtgtcgacgt ggggcgggtc aaggccattg
     2041 ccttgaatcc cactaaccca gaggaagttc tgctgaccat ggacattatg cgtggaacgc
     2101 cgatcaagac cgacacaatt gccgtccttg agacgcaggg gctcaccggt ctagccacca
     2161 tcaatttgac gggaggaagc cgagatgctc cctcgttgca ggcccaggag ggacaagctt
     2221 atcccgtcat caagaccggt ccttcgctct ttttccgctt ggatgaagcc gtctcccgtt
     2281 taatttcaga agaaggcctg gctcaactgt tggtcgatct tgatacggcg gcaaaagggg
     2341 cggcgaaggt cctggatgaa gacaatcgca ccatgctgaa gcgaacgatc aaggatcttt
     2401 cggatgtggc tcagaccatc gcggctcaca agactcagat tgagcaaagt ttgagcggtg
     2461 cggctaagag cgccgacaat cttgtcaagc tgacggcgtc gttgaatgcg gaggttccga
     2521 ccttactcgc cggaatcaac aagagcgtcg cggcgcttgg cacggcgaca gacgaactgg
     2581 ctcgcacgag caaaaccgtt ggagcggtca tcaatgaagc cagacccgaa ttgcaacagt
     2641 tcacccggcg gactttgccc gaggctagct tgttagtgac ggagctgcga cagctcacgg
     2701 gtacattgac ccgtgtggct cgggagttgg agcgagagcc gagctcgtta gtatttggac
     2761 gaaagtcccc gtcacgcggg ccgggggagt aacgcgggtt tgtgtacggt catgagatga
     2821 tgaactatcg actggcacat gtcgtgtgtg tgctgctggc attgacggtg gctggttgcc
     2881 tgtcgccccg catggattct tcggagatcc atacctatca attgagtctt gatggatggc
     2941 ccagcgagtc ccgcccaggc aagcttgatg gccccgtgct gctcgtgagt ccgcctcaag
     3001 ccgagccagg atttgagacg cagcgcatgg tgtatgtcaa gcggccctac gaactggaat
     3061 actatgcgtt gaatcaatgg gccgatacgc ccgtgcgcat gttcgctccg ttgatgatcc
     3121 aggcgctcaa tcagaatgat gtgtggcgtg ccgtgatccc actgccaagc tcgatccatg
     3181 gagactatcg tcttgatacc cacggatttc tgttacaaca ggaatttctc caacaaccca
     3241 gtcgcgtaag ggtgatggtc cgcacacagc tggtcgacct gaaagagtcg acgatcttga
     3301 atactcggac ttttgaggtc gtggagaacg ctacgagcga gaatccatat ggaggtgtcc
     3361 aggcttccaa ccgagcgatt gccgggttgc tcgatcaaat cggctcttgg cttcggcaat
     3421 gcgtccagca ttcgcctgag tgtctcgctg atggagctgg ttcgggattc attggaaaga
     3481 acaaaccgtg aacagcaacg acctttctat cctgtaaggt taactgtgat tgtttcttcc
     3541 gagcttcgct gccgatgaat ggaaacctgg aacgaagccg gcagtactat gagaagaacg
     3601 attacaggtg ctcgaagccg cacagtttaa ccgcatctag ctgctgagca gccaccgctg
     3661 cttcccatcc cttttcacaa ggaagaagac ggacccgcaa cgtatgtggg aactgagtac
     3721 ccaatcgggt ccgaacgtct gtgccgactc cgtccgtctc gaagaagatc tcgcagtcgg
     3781 gcagtccccc gaggccgata ccttgtgcct tgagcagcgc ttctttggcg acccagtagc
     3841 ggaagaatct cgttgcgcgc cgctcctcag tcacctgcat gatcgcagta tgttcggagg
     3901 aagtaaaaaa gcgcttggat agattcgcca cctcgatatc cgcacgaacg aattcaagat
     3961 cgactccgac ctcttgcact tttgagacgg caatgagcgc acggtcatgg gcatgggaca
     4021 aattgaacgt gatcgccggc cgatctcgta attccctttt taagaagggt ttgcctattg
     4081 cactgcgacc cagcggcacc gcatccgggt tgactcccag atacctgctc aacattgccc
     4141 tgaggcctcc gtgagccagc acgtagcgct gccgggtgtc ctcacgaaca aggcgcgccg
     4201 ctcgatcccg ctcgacgtca tccaaccagc ctgtgcatcg ctctagacag cgtcgcgatc
     4261 cgttgagctc gatcccccag aggtgaacgg cgtttggttc aagatggata tgatcgagct
     4321 gacttatatc agtgaggcgc tcaatggata ggaacgaaat caacacattg ctaccttgat
     4381 gccgacatcg cattggttcg taaaggaagt ggcg
//
LOCUS       DASTWE010000752         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1377872, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000752 DASTWE010000000
VERSION     DASTWE010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377872"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFT30_07785"
     CDS             <1..373
                     /locus_tag="VFT30_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5092569.1"
                     /translation="TGILGVATILLIGMMFGVGLTAISMAMAFSVKSHGDFFSVLGFL
                     SLPMIFLSSALVPLTAMPGWMSFLAQFNPMTWAIDAVRPLILSGWTEALPHVGMVIVV
                     MLVFDALCLYGGAKAFRRAMG"
     gene            476..1333
                     /locus_tag="VFT30_07790"
     CDS             476..1333
                     /locus_tag="VFT30_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEU5092570.1"
                     /translation="MIPENQIRALIRLLSDEDDRIVRTISGRLIDIGPSAVPLLQEAE
                     IEQPEMADRIASVLEEIRGSKLEEELAGMMALSDEAMSLETGAFLIAHYAYPTLDVAR
                     YQKQLDTMAGEVRARIGHRASGEEAVNALNRYLFTEQGFKGNTKNYYEVENSYLNCVM
                     DRRVGIPISLSTVYLLIGQRLALPLFGIGMPGHFLVKYESDRYNIFIDCFNGGALLTE
                     KNCARFLTEAGYGFDDKYLQKSPVRAILSRMIKNLLAIYSKSGDAGKTARLTKFIEIL
                     GGAPREEGL"
     gene            complement(1335..>1541)
                     /locus_tag="VFT30_07795"
     CDS             complement(1335..>1541)
                     /locus_tag="VFT30_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092571.1"
                     /translation="DCSFPANRPGPAHLHAGQCGQVAKEASIGQLVLSHFYPIADRYD
                     VRAQAGEQYGGKIWKGKDLLMIPV"
BASE COUNT          339 a          426 c          415 g          361 t
ORIGIN      
        1 gactggcatt ctcggcgtgg cgacgatttt gctgatcggc atgatgttcg gcgtcggcct
       61 gacggccatc tccatggcga tggccttctc cgtgaaaagt cacggcgatt tcttctccgt
      121 attgggattt ctttcgttgc cgatgatctt cctcagctcc gcgctggttc cgttgacggc
      181 gatgccgggc tggatgagtt ttctagcgca attcaacccg atgacctggg ccatcgatgc
      241 ggtgcggcct ttaattctgt ccggctggac tgaagccttg ccgcacgtag ggatggtgat
      301 cgtggtcatg ctcgtgttcg atgccctgtg tctgtacggc ggggccaagg cattccgccg
      361 tgcaatgggg taatctcacc atgaacatgg atcgcttgac tgacgaccaa atccgtcgga
      421 tcattacctt gataccgcta tacatcagct tgtgagcgac ggagagtcgt aggagatgat
      481 ccccgaaaat caaattcgtg cgcttattcg tctcctttcc gatgaggacg accgaattgt
      541 ccgtaccatc agcgggagac tcatcgatat cggcccatcg gctgtccccc ttctccagga
      601 agcggaaatt gagcaacctg aaatggccga tcggatcgct tccgtcctcg aggaaatccg
      661 tggaagcaag ctggaagaag aactggcggg catgatggct ctctcggacg aagccatgag
      721 cctggaaacc ggcgccttcc tgatcgctca ttacgcctat cccacgctcg atgtcgctcg
      781 ttatcagaaa cagctcgata caatggcagg agaagttcga gcacgtatcg gccatcgagc
      841 gtccggggaa gaagccgtca acgcactcaa tcgatatcta tttactgaac aagggtttaa
      901 ggggaacact aaaaactact atgaggttga gaatagctac ctcaactgcg tcatggatcg
      961 gcgagtcggc atccccatca gcctgtcaac ggtctatctc ttgatcggac agcgtttggc
     1021 actcccgctg ttcgggatcg gcatgccggg acatttcttg gtgaagtacg agtccgaccg
     1081 gtacaacatc ttcatcgact gtttcaacgg cggagccttg ctgaccgaaa aaaactgcgc
     1141 ccgttttctg accgaggcgg gatacggatt cgacgataag tacctgcaaa aaagtcctgt
     1201 tcgagcaatt ttgtctcgca tgatcaagaa cctcttagcc atctactcca aatccggtga
     1261 tgcgggaaaa accgcccgcc tgacgaagtt catcgagatc ctcggcggcg ctcctcgcga
     1321 agaggggctt taggttagac aggaatcatc aacagatcct tccccttcca aatctttcca
     1381 ccatactgtt ctcccgcttg agctctgacg tcataccgat ccgcaattgg atagaaatgc
     1441 gagaggacaa gttggccgat gctggcctcc ttcgccactt gcccacactg tccggcatgc
     1501 agatgggcgg gtcctggacg attggccggg aacgagcaat c
//
LOCUS       DASTWE010000753         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1151734, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000753 DASTWE010000000
VERSION     DASTWE010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151734"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFT30_07800"
     CDS             <1..729
                     /locus_tag="VFT30_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5092572.1"
                     /translation="PAVRTAHRTGIIALTNLLHAFGITTPLADNLSLALGSSSVSLLQ
                     ITSAYAGLANGGVVIHPVSLSNMVREGRETIWSPPLDRRQAATPQGAFLITSLLKGVM
                     DRGTGAKARALGVRGPVAGKTGTTDGYRDAWFIGYTPDIAIGVWVGFDDERPIKLTGA
                     QAALPIWSELAVRLIPRDPEDFETPVGVVQRKIDPKSGQLATSQCPEKRFEFFIAGTE
                     PTVYCEIHGGGFWEQMKQTFGLPQ"
     gene            834..1277
                     /locus_tag="VFT30_07805"
     CDS             834..1277
                     /locus_tag="VFT30_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5092573.1"
                     /translation="MKKTGYVDDGNITLLAKGVELRGEIKVDGTVRIDGRLEGDVHTK
                     GEVIVGEDGVVKGTIHADSLISSGRIKATVTATGKIQLLKTAILIGEVHCPMMLMEEG
                     AKFQGVSDMGVTGWPEEAPRLPSNVRDMNAHRGKAVALIGREPDL"
     gene            1407..>2109
                     /locus_tag="VFT30_07810"
     CDS             1407..>2109
                     /locus_tag="VFT30_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5092574.1"
                     /translation="MTRQQIFSIVFFSLLVLLLYQIGLMFKPFVFSALWAGLLAHWAF
                     PLHVRLTKLFGGKDALSAGLLTVGALGIVVVPLVAMGMMLVREAGAAEQEIRLWISAG
                     GLQRLPDQMAAVPFIGGWLKSAVSGTSTPALSLEQSLMTGVKELSQFLVGGMGGLLKN
                     TFALVTNFFIMLLVLFFLFTDGRQWLAVLYDLIPMEESHKLKILTRLDQTIRAVVKGM
                     LVTAIVQGLLAGMAYL"
BASE COUNT          447 a          515 c          628 g          519 t
ORIGIN      
        1 cctgcggttc ggaccgcaca tcgaactgga attattgcgc tcacaaatct actgcatgcg
       61 ttcggaataa ccaccccttt ggcggacaat ttgtccctgg cgctgggtag ttcctcggtt
      121 tcattgcttc agattacgtc agcctacgcc gggctcgcca atggaggcgt tgtcatccat
      181 ccggtctcgt tatccaacat ggtgcgtgag ggaagggaga ccatttggag tcctcccctt
      241 gatcggcgtc aggccgccac cccgcaaggc gcgtttctca ttacatcgtt gttgaaaggg
      301 gtgatggatc ggggcacggg ggccaaggct cgagcgttag gggtgcgagg tccagtggca
      361 ggcaagactg ggacgaccga tgggtatcga gacgcctggt tcataggata taccccggat
      421 attgcgatcg gcgtttgggt cggttttgat gatgagcgtc ccatcaaatt gaccggcgca
      481 caggcagccc ttccaatatg gagtgaattg gccgttcggc tcatcccgcg ggatcccgag
      541 gattttgaga cgccggttgg agttgttcag cggaagatcg accctaaaag cggacaactc
      601 gcgacatcgc aatgtccgga gaagagattt gagttcttca tcgctgggac cgagccgacg
      661 gtttattgcg aaattcatgg aggcgggttc tgggagcaga tgaagcagac gtttggttta
      721 ccgcaataag ttgaaggggc agtacaaagg tgtcggattc cgcaaactgt taggtatatc
      781 gccggtccta aggtaccctt tgggaaaatt gggtccgcac caaggagtcc cccatgaaaa
      841 agaccgggta tgtagacgat ggcaatatta cgctgttggc aaaaggtgtc gaactgagag
      901 gtgaaatcaa agtcgacggt accgtgcgaa tcgatgggcg tctcgaaggc gatgtgcaca
      961 cgaaggggga agtcattgtc ggggaagatg gagttgtgaa aggaaccata cacgccgatt
     1021 cactgatcag cagtggacgt atcaaagcca cagtgactgc caccgggaaa atccagctcc
     1081 tcaaaactgc gattctgatt ggcgaagtcc attgtccgat gatgttgatg gaagagggag
     1141 ccaagtttca gggggtcagc gatatggggg tcacaggctg gcccgaagaa gcccctcgat
     1201 tgcccagcaa cgttcgcgac atgaacgcgc atcgaggaaa agcggtcgcg ctgattggaa
     1261 gagagcccga tctgtaagcg aaccctgctt cttgctctca gtgctgtcct cacatcggtt
     1321 aggtctcgcc attctttcct gccgtgacag acgccgtcag atctgctatt tactcgatac
     1381 ctcttcaaga gactctgaca tcttccatga ctcgacaaca gatcttttcc atcgtgttct
     1441 tttctctcct cgtattgctc ctctaccaga ttggactgat gttcaagcca ttcgtgttct
     1501 ccgccttgtg ggcgggcctg ttggcccatt gggcatttcc tctccatgtc cgtttgacga
     1561 aactgttcgg tggtaaggac gcactctctg ccggcctttt gaccgtcggt gcgttgggga
     1621 ttgtggtcgt tcccttggtc gccatgggca tgatgttggt acgggaagcc ggagcggcgg
     1681 aacaggaaat ccgattatgg atttcagctg gcgggctcca gcgtctaccg gatcaaatgg
     1741 cggcggttcc cttcatcggc ggctggttga agtcagcggt gtccggtacc agtacgcctg
     1801 cgctttcctt ggagcagtcg ttaatgaccg gagtgaagga actcagtcaa tttctcgtcg
     1861 ggggaatggg aggtctgttg aagaacacct tcgcgctcgt gacgaatttc ttcattatgc
     1921 tgttggtgct gttttttctc ttcacggatg gccggcagtg gctcgctgtc ttatacgatt
     1981 taattccgat ggaagagtca cataagttaa aaatcctgac tcggttagat caaaccattc
     2041 gggctgtggt gaaagggatg ctggtcacgg cgatcgttca aggtctcctg gctgggatgg
     2101 cctatttgg
//
LOCUS       DASTWE010000754         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1248566, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000754 DASTWE010000000
VERSION     DASTWE010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248566"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFT30_07815"
     CDS             <1..1133
                     /locus_tag="VFT30_07815"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEU5092575.1"
                     /translation="PHRIPTIFDQCLIHWYNFLSHLPQKESTMNTAGRTRPRPALSRD
                     EILHQISVLLDSREDDLHAVLMTELLTGLLKLHDAHLDLLDVKIVNRAVKELRHAFSI
                     FHGYRNRQKVSIFGSARTLSDDPNYQLAYQFAQSIVREGFMVITGGADGIMRAAQEGA
                     GRENSFGVNIMLPFEQGPNSTIADDPKLITFKYFFTRKLMFQKEANAIALFPGGFGTH
                     DEGFEILTLAQTGKSDPQPIICLQAPGCDYWDDWSAFVTRQLLKRQLINEEDMSLFTI
                     VNSAEAAVSQILRFYRRYHSMRFVGRELAMRLKQTITPEQLEQLRDRFADLLSDGTFE
                     LRGPLAEELDEPALRDLPRLVFNFNRRSTGRLRQLISYLNEL"
     gene            1212..2084
                     /locus_tag="VFT30_07820"
     CDS             1212..2084
                     /locus_tag="VFT30_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEU5092576.1"
                     /translation="MSNISSAPPFHGSPDIVLYHAECSDGFGAAWALWKKFPSASFLP
                     VKHGHPPPPNLKDRRVVIVDFSYARPILEVMASETKELLVLDHHITAERTLDGFSDAY
                     FDQTKSGAVLSWEWAHGTAPPWLLQYIQDKDLWTWSLPESREINAALASYPFDFNVWD
                     SFTQSTLEQEGRAILRYEQELVGKLSAQASMVEFQGIVVPAVQSSILTSQIGERLSPH
                     HPFCLIWHDRDGRRYFSMRSRSDGTDVGTIAVSFGGGGHTHAAGFSVPLEMDGTPPTD
                     PRLPRTITDRRRTP"
BASE COUNT          494 a          662 c          546 g          509 t
ORIGIN      
        1 ggccacaccg gattccgaca atctttgacc aatgtctgat ccattggtac aattttctgt
       61 ctcacttacc gcaaaaggaa tcaaccatga atacagccgg cagaactcgg ccccgtcctg
      121 ccttatcacg agatgagatc ctgcatcaaa tcagcgtcct tctcgacagc cgtgaggacg
      181 acctgcatgc cgttctcatg acggaactcc tgacgggact gctgaaactc cacgacgcgc
      241 acttagacct cctggatgtg aaaatcgtga atcgtgccgt caaagagctg cggcatgcat
      301 tcagtatctt ccacggatat cgcaatcgcc agaaagtcag cattttcggt tctgcacgaa
      361 ctctttccga cgatcccaat tatcaactag cctaccaatt cgcccaatcc atcgtccggg
      421 aagggttcat ggtcatcacc gggggagcag atgggatcat gcgggctgct caagagggtg
      481 ccggccgcga gaacagcttt ggggtcaaca tcatgcttcc atttgaacaa gggcctaatt
      541 ccacgatcgc cgatgacccg aagctcatca ccttcaaata ctttttcacc cgcaagttga
      601 tgtttcaaaa ggaagcgaac gccattgcgc tcttccctgg cgggttcggc acacacgatg
      661 aaggttttga aatcctcacg ctggcccaga caggcaagag tgatccgcaa ccgatcatct
      721 gccttcaggc tcctggctgc gactattggg acgattggtc ggcctttgtg acaaggcaac
      781 tgctgaagcg gcagctgatc aatgaagaag acatgagtct ctttacaatc gtcaactccg
      841 cagaagccgc ggtgagccag atcctcagat tctaccgtcg ctaccattcc atgcgctttg
      901 tggggcgaga gctcgcgatg cgactgaagc agaccattac gcctgagcaa ctcgaacagc
      961 ttcgggaccg attcgccgat ctcctgtcgg acggcacgtt cgagcttcgt ggccccctcg
     1021 cagaagaatt ggatgaacca gccctgcgcg accttccccg actcgtcttc aattttaacc
     1081 ggcgtagtac cggtcgactt cgccaactga tcagttacct gaacgagttg tgagccatgc
     1141 attaacagta ggcatggaca cgacagacgt cttttagtcc tggcgcgggc atgctattct
     1201 ctcccgactc tatgagtaac atatcctccg cacctccatt ccatggttct ccggacatcg
     1261 tcctgtacca tgccgaatgt tccgatgggt tcggagccgc ctgggctctc tggaaaaaat
     1321 ttccgagtgc gagcttcttg cccgtcaaac acggccaccc gccccctccc aacctcaagg
     1381 atcgccgtgt tgtgatcgtc gactttagct acgcccggcc gatcctggaa gtcatggcct
     1441 ccgagaccaa ggagctcctc gttctggatc accatatcac tgcagagagg accttagatg
     1501 gcttttcaga tgcgtacttc gatcaaacaa agtccggtgc agtcctgagc tgggagtggg
     1561 cccacggcac ggcacctcca tggctacttc agtatattca ggacaaggat ttatggacct
     1621 ggtcgttgcc ggagagtcgc gaaatcaacg cggctttggc ctcctacccg ttcgatttca
     1681 atgtatggga cagctttacg cagtcgacgc tcgagcagga ggggcgagcc atcttgcgct
     1741 acgaacaaga gctcgtcgga aaactctcag cacaggcctc gatggtggaa ttccagggta
     1801 tcgtcgtccc tgctgtgcaa agctcaatct tgaccagcca aatcggcgag cgtctgtcgc
     1861 ctcatcatcc cttttgcctg atctggcatg accgtgacgg ccgtcgctac ttcagcatgc
     1921 gctcccgatc cgatggtacg gatgtcggta caatcgccgt ttcattcgga ggcggaggcc
     1981 acactcatgc tgctggattt tccgttccac ttgaaatgga cgggacacct ccgactgatc
     2041 ctagactgcc tcgtacgatc acggatcgcc gccgaactcc ataatttcgg actatcgtga
     2101 ttccacttca cgacgataat cctactcaac ggactcccat cgtcacgatc atcctcatcg
     2161 gcatcagcat cgctgtgttt ctctatcaag tgaatctccc tcaggaggct g
//
LOCUS       DASTWE010000755         1285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_343800, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000755 DASTWE010000000
VERSION     DASTWE010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343800"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFT30_07825"
     CDS             <1..691
                     /locus_tag="VFT30_07825"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEU5092577.1"
                     /translation="FAVKVRTGQSLNQIRFVCGEATVQDRALQALHRNTPLLYHNVTS
                     QKTVGNGDFRVERGLFLRIDLAGTDQSDNRVIGYRAKKNSHVIDLEKIGHYAAADFWE
                     PLHRHRHDSLLLEPEEFYILASKERIRVPAGYAAEMVAYEAACGELRTHYAGFFDPGF
                     GYGSKGEIKGTQVVLEVRPHDVPFLIHDGQTFFKVVYDKMMGMPTQLYGSLLGSSYQQ
                     QTLTLSKHFKV"
     gene            694..1101
                     /locus_tag="VFT30_07830"
     CDS             694..1101
                     /locus_tag="VFT30_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092578.1"
                     /translation="MAPSDLPQDDSLHPSRPTEPEEQGGGNEHQMHVIGLMVGTALMF
                     IGFLNVFLSISGGFEINVMPFLIYFAGLAIWANAAIETPTLRYGVMAIAIALALGLFQ
                     YGEVLFWHKQVLFWVTIIIVMYFMFVEPKKPTT"
     gene            1098..>1285
                     /locus_tag="VFT30_07835"
     CDS             1098..>1285
                     /locus_tag="VFT30_07835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5092579.1"
                     /translation="MTSVTISVIIPTLNEEKSLPRTLACLSESALTEIIIVDGSSTDG
                     TLSLAQEFCARTANARII"
BASE COUNT          291 a          356 c          331 g          307 t
ORIGIN      
        1 gtttgccgtc aaagttcgca cggggcagtc gctgaaccaa atcaggtttg tgtgcggcga
       61 ggccactgtt caggatcgag cgcttcaggc gctgcatcga aacacgccat tgctgtatca
      121 caatgtgaca tcccagaaga ccgtaggcaa cggagacttc cgtgttgagc gaggactgtt
      181 cctccgtatt gacctcgccg gcacggatca gagcgacaac cgtgtgatcg gttaccgcgc
      241 aaagaaaaat agccacgtca tcgatctcga gaagatcggc cattatgctg cggccgattt
      301 ttgggaaccg ctccatcgcc atcgccacga tagcttgtta ttggagccgg aagagtttta
      361 tatcctggcc tcgaaggaac gcattcgtgt gccagccggc tacgccgccg aaatggtggc
      421 ctacgaagca gcctgcggag agctacggac acactatgct gggtttttcg accctggatt
      481 cggctatgga tcgaagggag agatcaaagg cacccaggtt gtgctagaag ttcgtccaca
      541 tgacgtgcca ttcttgatcc atgatggaca gacctttttc aaggtcgtct atgacaagat
      601 gatgggaatg ccgacacaac tctacgggtc cctgttggga tcatcctatc agcaacaaac
      661 tttgacactc agtaaacatt ttaaggtcta accatggctc cgtccgatct gccacaagac
      721 gactctctgc acccgtcacg tccgacggaa ccggaagagc agggaggtgg gaatgagcat
      781 caaatgcacg tcatcggctt gatggtcgga accgcgctga tgttcatcgg ttttctcaat
      841 gtgtttctgt ccatcagcgg cggcttcgag atcaatgtca tgccgtttct catctatttc
      901 gctggtcttg cgatctgggc caatgccgcc atcgaaactc cgactctccg ctatggagtc
      961 atggccattg cgatcgcctt ggccttgggt ctctttcaat acggcgaagt cctcttttgg
     1021 cacaagcagg tcctcttctg ggtcaccatc atcattgtca tgtacttcat gttcgtcgag
     1081 cctaagaaac ccacgacatg acctcggtga cgatttccgt catcatccca actttgaacg
     1141 aagaaaagag ccttccacga acccttgcgt gtctatcgga gtctgctctc accgagatca
     1201 tcattgttga cggaagcagc actgacggca ccctcagcct ggctcaggaa ttttgcgcgc
     1261 gcacggccaa tgcccgcatt atcat
//
LOCUS       DASTWE010000756         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1087181, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000756 DASTWE010000000
VERSION     DASTWE010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087181"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1183)
                     /locus_tag="VFT30_07840"
     CDS             complement(98..1183)
                     /locus_tag="VFT30_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092580.1"
                     /translation="MPKLGKQALEEYLRARFGPRVELRSYGVIGKESSKGAQKRYGYG
                     TPIKLTFRVGRRVRSAVLETMKPGPFGHEYMADRAQAMLWDYDSYGRLPRHVKALDVG
                     AFGANQALFSLAEAREFFVLNEWTDGASYHGDLDRLAKGGTLRKLDRQRTVALAHYLT
                     RIHAKKRRDADLYKRRLRELIGHGECIMGLTDSYPSHCGFITGDLLRTVEEACNRWRW
                     RLRDKTNRLSQVHGDFHPYNVLFRTGTDFAVLDRSRGEWGEPADDITAMTINYLLSSL
                     IRWGKLRGPFEVLFRLFWDTYVETSGDKEVTETAAPFFAFRGLVVASPLWYPKLSIDI
                     RRRLFHFIENVLDVQCFEPERVNEYCQ"
BASE COUNT          322 a          395 c          340 g          274 t
ORIGIN      
        1 agcgcttcaa gctgcggctg cagcgcggca gtgatcgtgc tctttcccga agcaggcaaa
       61 ccggtgagcc aaatagcaat ggggctatgt tccctcatca ctgacaatac tcattcactc
      121 gttccggttc aaagcattgc acatcgagga cattctcgat gaaatgaaac agacgccgtc
      181 ggatgtcgat cgacaacttg ggataccaga gcgggcttgc cacgacgagg ccgcgaaaag
      241 cgaagaaggg tgcggccgtt tcagtaactt ccttgtcgcc gctggtttcc acatatgtat
      301 cccagaacag ccggaacaag acctcaaatg gaccccggag cttgccccat cggatcaagg
      361 aactcaggag ataattgatc gtcatcgcgg tgatgtcgtc ggctggctcg ccccattctc
      421 ctcgcgatct gtccaacacc gcaaaatccg tcccggtacg gaacaacacg ttgtacgggt
      481 gaaaatcccc gtgaacctgg gaaagccgat tggttttgtc acgaaggcgc caccgccatc
      541 gattgcaggc ttcctccacg gttcgcaaca aatcgcccgt gataaagcca caatggctgg
      601 ggtaactgtc ggtcaacccc atgatacatt caccatgacc aatcaattca cgcagccgac
      661 gcttgtagag atctgcgtct cgccgtttct tcgcatggat ccgggtcaag tagtgcgcta
      721 atgcgaccgt acgttgccga tccaatttac gcaatgtccc gcctttcgcc aaccgatcga
      781 gatccccatg gtagctcgcc ccgtcggtcc attcattcag cacgaagaat tcccgggcct
      841 ccgcgaggga aaagagagcc tggtttgcgc cgaatgcacc cacatcgagg gccttcacat
      901 ggcggggaag gcgcccatag gaatcgtagt cccacaacat ggcttgagca cggtccgcca
      961 tatattcatg cccgaagggg ccgggcttca tcgtttcaag cacggcggaa cggacccgac
     1021 gtccgactct gaaggtcaat ttgatcggtg taccgtaccc ataccgcttc tgcgcccctt
     1081 tggaactttc tttgccgatg actccatagg accgcaactc aacacgaggg ccaaaacgcg
     1141 cccgtagata ctcttccagc gcttgcttgc ccaactttgg cataccgcta catcacgcaa
     1201 gtcatattcc agcttcacag gcgtgagatg actcatagaa ggcaaggtcg agagaaaagg
     1261 actgtcgcat tgagcaccac cgatcgggca tataactgtg ggaaaatgcg ccacagcaaa
     1321 atcggtttaa g
//
LOCUS       DASTWE010000757         1492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_860853, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000757 DASTWE010000000
VERSION     DASTWE010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860853"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="VFT30_07845"
     CDS             complement(<1..1312)
                     /locus_tag="VFT30_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU5092581.1"
                     /translation="MDDHSTIRRVMVGTDRSKTADRAVLWAAQFAKHYGAELFVVQVI
                     LPQHPSTTEFGAAEHTRAAVANDELENFVRQIAGERGHSLAVIDSDPASAILRAAERE
                     AVDVLVVGNSGMAGRKQFLLGNVPNRISHNSRCTVVIVNTQSAAEEPGAEFVHVSKPQ
                     VECAASQPRLGARAAHIATVMAKHGLKELFGRPEESDVSISYQQAKRLRAALEELGPT
                     FSKLGQVLSTRPDLLPPEYIEELAMLQSHVPPMPESAVVQVAEQELGVPWEDVFESID
                     PKPLAAGTIAQVHRATLENGDRVVIKVQRPTARAEIEQDLALLEIFAQKVGQRPALNQ
                     VVNMEAVFKHLSTSLHRELDFHQEIGNIDQMRTVLADYDRLAVPAVHHKLSTARLLVM
                     EEIQGIPIAQAPEGPDRIEAARQLLESYYKQIIVDGFFHADPHPG"
BASE COUNT          304 a          412 c          429 g          347 t
ORIGIN      
        1 tacccgggtg gggatcggcg tgaaagaagc cgtcgacgat aatctgtttg taataactct
       61 ccaagagctg ccgcgccgct tcgatacgat ccggcccttc cggtgcctgt gcgatcggaa
      121 tcccttgaat ctcttccatt accaagagac gagcggtcga tagcttgtgg tgcaccgccg
      181 gtacggccag ccgatcatag tctgcgagaa ccgtccgcat ctgatcgata ttgccgattt
      241 cttgatgaaa gtcgagttcc cggtggagcg acgtggagag gtgcttgaaa acggcttcca
      301 tattcacgac ttgattgagg gctgggcgtt gcccgacctt ttgagcaaat atttcgagca
      361 aggcaagatc ttgttcgatt tcagctcggg cggttggtcg ttgaactttg atgaccactc
      421 ggtcaccgtt ctcgagtgtg gcccgatgaa cttgcgcgat ggtcccggcc gcgagtggtt
      481 tgggatcgat ggactcaaac acatcttccc acggcacccc taattcttgc tcggcgacct
      541 gaacgaccgc actctcgggc atcgggggaa cgtgactctg caacatggcg agttcttcga
      601 tgtattccgg agggagcagg tccggccgcg tcgacaatac ttgcccaagc ttcgagaagg
      661 tcggccccag ttcctctagt gcggcccgca ggcgcttggc ttgttgatag ctgatggaca
      721 catccgattc ttccggccgg ccgaacaatt cttttaagcc atgcttcgcc atcactgtgg
      781 cgatgtgcgc ggctcgggct cccagacgtg gttgagaggc tgcgcattcc acctgtggct
      841 tggaaacatg cacaaattct gcgccaggct cctctgctgc cgactgggta ttcacgataa
      901 caaccgtgca gcgggaattg tggctgatcc ggttgggcac attaccgagt aagaattgct
      961 ttcgtccggc cattccggaa ttacctacaa ccaagacatc gactgcctcc cgctcagcgg
     1021 cgcggaggat tgccgaggcc ggatcgctat cgatgacggc aagcgaatgt ccgcgctccc
     1081 cggcgatctg tctcacgaag ttttccagtt catcgttggc aacggcagct ctggtgtgtt
     1141 cggctgcgcc gaattcggtg gtggaggggt gctgaggcag gatgacttgc accacgaaca
     1201 gctctgcgcc gtagtgttta gcgaattggg cggcccacaa gacggcccga tcggccgttt
     1261 tcgaacgatc ggtgcccacc atgacacgtc gtatggtgct gtgatcgtcc ataatcctta
     1321 tgaactcacc gacgatgata agacgtcaag tatgaccatt gcctcaattg catgtcaatt
     1381 attggaaaac cggtagaagg ctccgaacaa ggagcacgtc gattcattga cggcccatcg
     1441 gtcacgttcg tatgatcttc gccgtatgtt gaacgatcgc gaccaagggc aa
//
LOCUS       DASTWE010000758         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1604486, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000758 DASTWE010000000
VERSION     DASTWE010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604486"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..543
                     /locus_tag="VFT30_07850"
     CDS             391..543
                     /locus_tag="VFT30_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092582.1"
                     /translation="MEPARKLDYQQVWNRPSPGAPKTSKPERIFDEQGNEIQPWEIKL
                     GKSIFI"
     gene            complement(575..1282)
                     /locus_tag="VFT30_07855"
     CDS             complement(575..1282)
                     /locus_tag="VFT30_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092583.1"
                     /translation="MKLPDRIYHLAEASNWPSIRCHGLLSASRLIKATELTKTDRDRL
                     ERGQRLTHTELSGGVQIRDQRPMSPAALKRCLCDMEPADWYAMINARVFFWLDVDRLN
                     RQKAACEPRPQVVMAVNTAALVAAYEKRVAVTPINTGNARRKPALRGVATFVPLAEWV
                     RSGWASEAAALGIPSRKESHQPVELTVTDAVPDIMRFVVGIFALPSGQSFDERRRVGK
                     IDAIGPTPWSAGALNAV"
BASE COUNT          359 a          449 c          367 g          347 t
ORIGIN      
        1 acattcggcc cgctgaacgc gtgacctggc tctaatcccc cgactcgcgc gcggaggctc
       61 accgggcggc gtcctcccca ggacgccgcc ctttcttttt ctgaatatag acggatcgca
      121 cgtatgaact atcattacgt gtcccaggat taagtcatga gaagaactgt ggttttcagg
      181 cactcactgt tatctgtcca ttgacttggc tcgcctcgtt tccggttcat agcggacttt
      241 cctctacttt ctcatagtcg ccttactttc aaagtagctt tcacaacaac cgcaagaggt
      301 taccccagct ctttttgcaa cgagcatgtg tgtaaactac ggtcatgcga cctcatacat
      361 cacccttcaa catcatgagg aggtctcgca atggaacctg caagaaaact cgattaccaa
      421 caagtctgga acagaccatc gcccggtgcg ccgaaaactt ccaagccgga acgcattttt
      481 gatgaacagg gaaatgagat tcaaccgtgg gaaatcaagc taggcaagtc tatctttatc
      541 tgacgtcgct atgggcatct ggttcttcta ttggctaaac ggcattcaat gcccctgctg
      601 accaaggggt aggcccaata gcatcgatct tgcctaccct tctcctttca tcaaacgact
      661 gaccggatgg taatgcaaag atcccgacaa cgaaacgcat gatgtcaggc actgcgtcag
      721 tgacagtcaa ctcgaccggt tgatgcgact cctttcgtga agggatgcct aatgctgctg
      781 cctcgctcgc ccatcctgac ctgacccact cggctaacgg aacgaacgtg gcaactccac
      841 gcagcgcagg ctttcgacgc gcattcccgg tattgatcgg agtcactgcc actcgcttct
      901 cgtatgcagc aaccaaggcg gccgtattca cggccatcac gacctgaggc ctcggctcac
      961 aggcagcctt ctgacgattg agtcgatcca catcgagcca gaagaacaca cgcgcgttga
     1021 tcatggcata ccaatcggcc ggttccatgt cacacaagca ccgcttcagc gcagctgggg
     1081 acatcgggcg ctgatcgcga atctgcaccc caccggagag ttcagtatgt gtcagcctct
     1141 ggccccgctc caatcggtct cgatccgtct tggtaagctc cgtggctttt atcaaccggc
     1201 tggcggacag cagcccatgg catcggatcg acggccagtt tgacgcttcc gccaagtggt
     1261 aaatccggtc cggcaacttc atgaggactt tccagtctcg tcttcccgtg cacgaatcgt
     1321 aagacgtatt gtgtgaagag caacgagtga cggctacacg gaattcaacg ccgagacctg
     1381 gatctgttcg gtaccttaaa tgagaatcta gtcgtaatgc ctacaggacg tcattcacaa
     1441 tcatcattca gagttcaaag actatccctg gctcttgaaa aacaccgcag ccagggcggg
     1501 aacagtcacg gtcaatgaat gg
//
LOCUS       DASTWE010000759         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_247222, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000759 DASTWE010000000
VERSION     DASTWE010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247222"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..>1222
                     /locus_tag="VFT30_07860"
     CDS             153..>1222
                     /locus_tag="VFT30_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU5092584.1"
                     /translation="MRLNDKLPPSFQSIVKPAFEALSRAQVVDRLWAKDHRLWKPDPK
                     EIADRLGWLTVQNQMGQQLEQLQRCVATAKELNIKDVVLLGMGGSSLGPEVFRTVFGS
                     QKGFSRLWVLDSTIPGWVRQVTKAISPSRTLFLVASKSGGTIEVMSLFAHFWNLVTKT
                     KGNHGGRQFVAITDPGTGLEKMARDHGFGEIFTNPADIGGRYSVLSLFGLVPAALLGL
                     DIAKLLDRAADMAEQCRQQKDVETNPGAYLGVAMGSLAKTGRDKVTVIASPSLSTFGL
                     WVEQLLAESTGKEGTGLIPIAQEPVLRPRAYGIDRFFVYLKLKGDKNHRLDQAVQALV
                     KAKQPIIQFNLRDRYDVGAEFF"
BASE COUNT          275 a          330 c          331 g          286 t
ORIGIN      
        1 tggtctctgg tcgatggcat ggcgttggtc attcgccgcc cgactgataa gtagtaaagc
       61 gtatcgagtc cgtgttgcct tttctatacc ggctcaataa gatacgaacg agctactcac
      121 gaccccccac ctccggaagg aggcccatgt ccatgcggtt gaatgacaag cttccaccat
      181 cgtttcaatc catagttaaa ccggccttcg aagcgctcag ccgtgcgcag gtcgttgacc
      241 ggttatgggc caaggaccat cgactctgga agcctgatcc caaagaaatc gccgaccgtc
      301 ttggctggtt gacggtacag aaccagatgg gccaacagct ggagcaactg caacgctgcg
      361 tcgctacggc caaagaactg aatatcaaag atgtcgtgct tctgggaatg ggaggcagca
      421 gtcttggacc agaggtcttc cgtactgtgt ttggatctca gaagggcttc tcgcgtttgt
      481 gggtccttga ctcgaccatt cctggctggg ttcggcaggt cacgaaggct atctccccat
      541 cgcgcacact ctttctcgtg gccagcaagt ccggtggtac gatcgaagtt atgtcgctct
      601 tcgctcattt ttggaacctt gtgacaaaga ccaaggggaa tcatggagga aggcaatttg
      661 tcgcgatcac cgaccctggt accggtttgg aaaagatggc tcgggaccat ggatttggag
      721 aaatctttac caacccagct gatattggtg gacggtattc cgtgctttct ctgtttggtc
      781 ttgttcctgc agcactcctt ggcctcgata ttgccaagct cttagaccgg gcagccgata
      841 tggcggaaca atgccgccag cagaaagacg tcgagaccaa tcccggtgcg tatctgggcg
      901 tagccatggg cagccttgcc aagactgggc gcgataaggt gacggtcatc gcgtcaccgt
      961 ccctttccac gttcggtctc tgggtcgaac aacttctcgc tgaaagtacg ggcaaagagg
     1021 gtacgggtct catccccatc gcacaagaac cggttctgag gccacgcgcc tatggaatcg
     1081 accgattctt cgtctatctc aaacttaaag gagacaagaa ccataggctc gatcaagcgg
     1141 ttcaagcgct cgtcaaggct aaacaaccga taatccagtt caaccttcgt gaccgctatg
     1201 atgtaggtgc tgagttcttc ag
//
LOCUS       DASTWE010000760         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_990574, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000760 DASTWE010000000
VERSION     DASTWE010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990574"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFT30_07865"
     CDS             complement(<1..195)
                     /locus_tag="VFT30_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092585.1"
                     /translation="MTGKLVGSFIEELVAVCSAVPHHRIGIRRASCLFLEQVMKAVLV
                     WIRRGRIVAILKEVFSLILAQ"
     gene            127..>1354
                     /locus_tag="VFT30_07870"
     CDS             127..>1354
                     /locus_tag="VFT30_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU5092586.1"
                     /translation="MVWNGAAYSYQFLNERANQLARHLRSQGIAMESRVALCMERSLE
                     MVVGLLGILKAGAAYVPMEPLYPRERLGFMIRDSGAQLLLTQKRFVDDFQSQTVPIIA
                     LDETWPVVTAFPDIDCPWRTVPENLAYVLYTSGTTGEPKGIEISHRALINHSTAMAKH
                     YGLRTADRVLQFASISFDVAAEECFPTWTAGAAVVLRPNEPVPAFSDLQQFIEDHELT
                     VLNLPTPYWTEWIEVIEQSGTVLPPSLRLVIVGSEKALPDSLVRWQRLVGDRIAWCNS
                     YGPTETTITASYFVPERQENWISASTVPIGRPISNVELYVLDPVLQPVPIGVAGELYI
                     GGAGLARGYHRQPARTAEKFVPHPFSREPGARLYRTGDRVRRLADGNVEFLGRYDDQI
                     KIRGFRVEPAEIEFWMK"
BASE COUNT          289 a          366 c          408 g          291 t
ORIGIN      
        1 ttgagccagg atgagagaaa acacctcctt gaggattgca acaatacggc cgcgtcttat
       61 ccacacgagt actgccttca tcacctgttc gaggaacaag cacgacgcac gccgaatgcc
      121 gatgcgatgg tgtggaacgg cgctgcatac agctaccagt tcctcaatga acgagccaac
      181 cagcttgccc gtcatctcag atcgcagggc atcgcgatgg aaagccgcgt ggctctttgc
      241 atggaacgct ctctggagat ggtagtgggt ctgctgggaa ttctcaaagc aggggcagcc
      301 tatgtgccga tggagccact atatcctcgc gagcggctcg gttttatgat ccgggacagc
      361 ggagcgcagt tgttgctcac tcaaaagcga tttgtcgacg actttcagag tcaaacggtt
      421 ccgatcatcg cactggacga aacgtggcca gtcgttactg ccttccccga tatcgattgt
      481 ccctggcgca ctgttccgga gaacttggct tatgttctgt atacctccgg gacgacgggt
      541 gaaccgaagg gaatcgaaat ttctcaccgc gcattgatca atcacagcac agccatggcg
      601 aagcactatg gtcttcgcac ggctgatcgt gtgctgcaat ttgcctcgat cagtttcgat
      661 gtcgcggctg aggagtgctt tccgacttgg acggcagggg cagcggtggt gttgcggccg
      721 aacgagccgg tgccggcgtt ttcggacctc caacagttca tcgaggatca cgagctcacc
      781 gtgttgaatc tgccgacgcc ctactggacc gaatggatcg aagtgattga gcaaagtgga
      841 acggttcttc cgccctcact ccgcttggtg atcgtaggaa gtgagaaggc cctgccggat
      901 agtctggttc gctggcaacg gcttgtcggg gaccgcatcg cttggtgcaa ctcatatgga
      961 ccgactgaaa caacgatcac ggcgagctat ttcgtgccgg agcgacagga gaattggatc
     1021 tcagcatcta ccgtgccgat cggccggccc atttcgaacg tcgagctata tgtattggac
     1081 ccggtgttgc agccggtccc gatcggagtg gcgggcgaac tctacatcgg cggtgcgggg
     1141 ttagccagag gctaccatcg ccagccggct cggaccgccg aaaaatttgt cccgcaccct
     1201 ttcagccggg aaccgggcgc tcgtctgtat cgaacgggag acagggttcg tcggctggcg
     1261 gacggcaacg tggagtttct cggtcgatac gacgaccaga tcaagattcg aggatttcga
     1321 gtagaaccgg cagagatcga gttttggatg aagc
//
LOCUS       DASTWE010000761         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_990580, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000761 DASTWE010000000
VERSION     DASTWE010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990580"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFT30_07875"
     CDS             <1..282
                     /locus_tag="VFT30_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEU5092587.1"
                     /translation="PDPGDYHPEPEWYFLFYFQLLRLKLFAGEFGQFMGAVALPVAFM
                     ALLVALPFIDKDPERNIFKRPIALISWIAIMVVIVLFTVASVINREFLD"
     gene            323..>1113
                     /locus_tag="VFT30_07880"
     CDS             323..>1113
                     /locus_tag="VFT30_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092588.1"
                     /translation="MSPTKIGFGILWPACWTGIPIKAVFAVLAMTMGLVHFEGRFGLA
                     FLMLFASPVTVFAAPIIIAIFETQFGEGIGLPLIFGMSIPVDIWALGLVGRTFFLERL
                     RKEPPYDGLGFAIWWRAALIGAFFLPLLWWIVSRVTEIAITASHSMAEMDRMRHLFDT
                     GLPIAERIGLELTIWGSISSAVLIVLAVIGISILGQIIRRMAENSRPASENYQKLVTR
                     WDLMRVPTDQGLLLASLAGVGVVLSLVFWAILPVTTPHPHDCCKKP"
BASE COUNT          201 a          285 c          320 g          307 t
ORIGIN      
        1 ccggacccgg gggactatca tccagaaccg gaatggtatt ttttgttcta cttccagctg
       61 ctcaggctga aactgttcgc cggtgagttc ggtcaattta tgggtgccgt ggcattgccg
      121 gtcgccttca tggcattgct cgtcgcactc ccattcatcg acaaggatcc cgagcggaat
      181 attttcaaga gaccaatcgc tttgattagt tggattgcga tcatggtggt gatcgttttg
      241 ttcacggttg catcggtgat taaccgagag ttcctagact gatcctaagt acacgttggt
      301 tatggctgaa gaacgcgagt caatgtctcc aacgaagata gggttcggta tcctctggcc
      361 tgcctgctgg accgggattc ctattaaggc ggtgtttgcg gtccttgcca tgacgatggg
      421 attggtgcat ttcgaaggac ggttcggtct tgccttttta atgctgtttg ccagtcccgt
      481 cacggttttc gccgctccca tcatcatcgc catctttgag acgcagtttg gtgaaggaat
      541 cggtttaccg ctcattttcg gaatgtctat tcccgtcgac atctgggcgc tggggctggt
      601 cgggcgaacg ttcttcttgg aacggcttcg gaaagagcca ccttacgatg ggcttggttt
      661 tgcgatctgg tggagggcgg cgctcattgg ggctttcttt cttccgctcc tctggtggat
      721 cgtcagccgt gtcacggaga tcgccatcac cgcatcgcac tcgatggccg aaatggaccg
      781 catgagacat ctcttcgaca ccggtcttcc cattgcggaa cgaatcggtc tcgagctcac
      841 gatctgggga tccatctcgt cggcagtcct gatcgtcttg gcggtcatcg ggatatccat
      901 acttggacaa atcattcgac gcatggcgga aaactcccgt ccggcgtctg aaaattatca
      961 aaagctcgtg acccgctggg atttgatgcg cgtgccgacc gaccaagggt tgctgttggc
     1021 ttcattggcg ggggttgggg tggtgctgtc gctggtgttt tgggccatac ttccggtgac
     1081 gactccgcat ccgcacgatt gctgtaagaa acc
//
LOCUS       DASTWE010000762         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_893662, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000762 DASTWE010000000
VERSION     DASTWE010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893662"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..703)
                     /locus_tag="VFT30_07885"
     CDS             complement(245..703)
                     /locus_tag="VFT30_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5092589.1"
                     /translation="MRGKRLVMTVCSLVAVAVWSTAGWAAPEADPLKPRVPDAERGDV
                     RKMKSPLTVTPEILAKGKELYEGKGTCANCHGQTGAGDGAGGMLLSPGPRNFTNCKFH
                     KKRNDGELFWVVKNGSPGTGMPALVKGNVLTEEEAWTIIAYERTFCKDTE"
     gene            complement(976..1419)
                     /locus_tag="VFT30_07890"
     CDS             complement(976..1419)
                     /locus_tag="VFT30_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEU5092590.1"
                     /translation="MRFNKKIVSLVVAVAATWAFSGATSFAADRSLYERLGGVGAIQA
                     VVTKFIGNVGADKRINGYFAKADLKQLNKHLVDQVCMATGGPCTYSGRDMKTTHKGMK
                     ITSAAFGALVEDLVAALDTFNVPKKEKDELIAILAPMKGDIVEVP"
     gene            complement(1464..2411)
                     /locus_tag="VFT30_07895"
     CDS             complement(1464..2411)
                     /locus_tag="VFT30_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5092591.1"
                     /translation="MQRFRVLTLTLGLGVLLGASGCITMPGSAGAKVHDVTFTATESE
                     IVIDGTGTKYKAWTFNGQMPGPVVRVTEGDTVNFTLINPPTNAQGHSMDFHAAELDFL
                     KNYREIKPGETIKYTFVAKKPGVFFYHCGAPPMIQHIGRGMFGAIIVDPKDASVWPKA
                     DREFVLVQSELWKNPDNVQAMFDRKFDHQIFNGGVFKYHPFFPGSEPLEVKVGERVRI
                     YFVNAGPNEFSSLHPIAEIWDNVYESGNPHNKFTGVQTYVVGPGSAATFDLIADEPGA
                     YPVVTHSLTGALRGAIAVVIANQSPKKYDNLMPLTPWNP"
BASE COUNT          670 a          752 c          755 g          675 t
ORIGIN      
        1 cctcttttca tgaggaagga aggggggaaa gcgagagggg cttttaaagg gggctaacag
       61 gcgaacctat gcaatcaaat ttctcacggc gcgctaatcg aacgaactcc gcaagagttt
      121 gaaaaagaat gtcggaagac aatggaactg gggtttaaga atgatgcgat tcaaagcgag
      181 ctatcagtcg agatcatcga ctgatagctc gctttgaaga agcgaatccc gacttcgttg
      241 acagctattc ggtatcctta cagaaggttc gttcgtaggc gataatcgtc caggcttctt
      301 cttctgtaag cacgtttccc tttacaagcg ccggcatgcc ggtgccggga ctcccgtttt
      361 tgacgaccca gaagagctcc ccatcgttcc gttttttgtg aaacttgcag ttcgtgaagt
      421 ttcgagggcc cggcgacagc agcattccac cggccccgtc gcctgctcca gtctggccgt
      481 gacagttcgc gcaggtgccc ttgccttcat agagttcctt gcccttggcg agtatctcag
      541 gagtgacagt cagcggactc ttcatttttc gtacgtcacc acgttctgcg tccgggacac
      601 gcggcttcaa tgggtcggcc tcgggggcgg cccatccggc cgtactccac acggcgactg
      661 cgaccaagct gcacactgtc atgactaaac gcttccctct catgattcct ccttggtgat
      721 taccactgat gttacggtca catagctgct cgcgccgcgc gtttggaggc gcagataacg
      781 aagaatatga taacacgata tcgccttcag tgaagtaccc atgtctctgg aagtgcgtag
      841 atcgaagtcg aattggaggc tggtatagaa cattcacatg aaggcatgcg ggccgggact
      901 tcgtgaggtc ccggcccgca agagactcat gtgagatccg cagtgatcac cactgcatgt
      961 ctcacgcggg cgtcatcatg gaacttcgac gatgtcgccc ttcatcggtg caaggatggc
     1021 gatcaactca tccttttctt tcttcgggac gttgaaggta tccaatgcgg caaccaggtc
     1081 ctcaacgagg gcgccaaagg cggcggaggt aatcttcatg cccttgtgcg tcgtcttcat
     1141 gtcccgaccg gaataggtgc agggtccgcc ggtcgccata cacacctgat caactaagtg
     1201 cttattgagc tgcttgagat cggctttcgc aaaatagcca ttgatgcgct tgtccgctcc
     1261 cacgttgccg atgaatttgg tgacgacggc ttgaatggct cccactccgc ccagccgctc
     1321 atagagcgac cggtcagcag caaaggatgt cgcgccacta aaagcccagg tagctgcaac
     1381 tgccacgaca aggctcacta tcttcttatt gaatctcata tcgtcgttcc tttctatgaa
     1441 aagagattag cttttataac accctatgga ttccaggggg tgagtggcat caaattgtca
     1501 tacttcttgg ggctctggtt ggcgatcacc accgcaatcg cacctcgcaa ggctcccgtt
     1561 aaggagtggg tcacgaccgg ataggctcct ggttcatcag ctatcagatc gaatgtggct
     1621 gcgctgcctg gaccgaccac ataggtctgg acccccgtaa acttattgtg cggattgccg
     1681 ctttcgtaga cattgtccca gatttcggcg atgggatgga gagaagagaa ctcgttcggg
     1741 ccggcattca cgaaatagat tcgcacccgt tcaccgacct tgacttccaa cggctccgat
     1801 cccgggaaga agggatggta cttgaagacg ccgccgttga agatctgatg gtcgaacttc
     1861 cgatcgaaca tcgcctggac gttatccggg ttcttccaca gctcggattg caccaggaca
     1921 aattcccgat cagctttggg ccagacactg gcgtccttag gatcaacgat gatcgcaccg
     1981 aacatgccgc gacctatgtg ctggatcatc ggcggtgcgc cgcagtgata gaaaaacacg
     2041 ccgggcttct tggccacgaa ggtatacttg atcgtctcgc cgggcttgat ttcgcggtag
     2101 ttcttcaaga aatcgagttc cgccgcgtga aagtccatcg aatggccttg ggcgttcgtc
     2161 ggggggttga taagggtgaa gttgacggta tcaccttcgg tcactcgcac gaccgggcct
     2221 ggcatctgac cattaaaggt ccaggcttta tacttcgtac cggtaccgtc aatgacgatt
     2281 tccgattcgg tcgccgtgaa cgtgacatca tggacctttg ctcctgcgga cccgggcata
     2341 gtgatacacc cgctggctcc caacaggacg ccaagcccga gggttaatgt gagcacacga
     2401 aacctctgca tggtagcctc cctttagatt tttatggaaa agagacgctg cactgacgag
     2461 cgcggagaat tagagcgaat tagagccctc cacatcactc accaagcagg cgccgggtca
     2521 actatctacc aaattctgaa gaaaaaatac aaagaaaatt ttttgaagcg catttagcca
     2581 aggacattga ctctctctca tgaatggtgg ggcttcgtag tgtagcacaa tacataccac
     2641 cagagtcgtt actgagtaga gtctataatt ccgcgcaaaa ctaatgatct agcaggctgt
     2701 tggtgaagtt ttgaggttcc cttgaaaaga tcgacttgaa tggttcaagc ggttaggatc
     2761 aggagaagaa tctaataaga tacactctgt atctgattcg attcacaccg aagcgatctc
     2821 caaactggtt gagaagcatc gtgatgtaag ca
//
LOCUS       DASTWE010000763         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_893721, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000763 DASTWE010000000
VERSION     DASTWE010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893721"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VFT30_07900"
     CDS             <1..728
                     /locus_tag="VFT30_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEU5092592.1"
                     /translation="RVAMFERLNQQRGKTGPTPRRQLPLSVKTRLGYDSVTVEGWVEE
                     LLMEQPAVISLHGRTLRQMYRGMADWSAIERAAELVKGTGTLLLGNGDIQSLDDIAVR
                     VRETGVDGVLVGRGVFGTPWFFRSKEQARMRALGMNDTEVRQDPGDISLNERFAVLVD
                     HAQQFQALVGEKQFYRIRKHLGWYCKGFPHAASLRAQMVRVSSVKELHALLEDFYDRP
                     EAIGDPPQTESDDEPRVLVSRCS"
     gene            719..1351
                     /locus_tag="VFT30_07905"
     CDS             719..1351
                     /locus_tag="VFT30_07905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU5092593.1"
                     /translation="MQLVLASSSPRRRELLALLGLPFEVCPSEFHEHPMVGLSPIEQV
                     KRFAREKARSVALVRPRALVLGSDTVIDLESQLLGKPMDLVDARAMLARLAGRSHHVH
                     TAVALCDRERNIESVEVATAEVRMKADLDQAYERYLASEESLGKAGAYAIQGLGGDLV
                     ERIAGDYTTVVGLPLKLVAHLLRSAGYPIFVNVEDLYRRKPYANWNRFAA"
BASE COUNT          330 a          388 c          452 g          342 t
ORIGIN      
        1 cgcgagttgc catgtttgaa cgtctgaatc aacagcgcgg caaaaccggt ccaacccctc
       61 ggcgtcaact gcctctttcc gtgaagaccc ggcttggtta tgactcagtg acggttgagg
      121 gatgggttga ggagctttta atggaacaac ccgccgtgat ctcgcttcat ggacgaacgc
      181 tccggcaaat gtatcgaggc atggcggact ggtcggccat tgagcgcgcg gccgagttgg
      241 tgaaggggac cgggacattg ttattgggga atggggacat tcagagcctt gacgacattg
      301 cggtacgagt tcgtgaaacc ggagtcgatg gagtgttggt cgggcggggt gtatttggca
      361 cgccgtggtt ctttcgttcc aaagagcagg cccgcatgcg agcccttggt atgaatgata
      421 ctgaggtcag gcaagatccc ggcgatattt cgctgaacga gcgcttcgcc gtgttggtcg
      481 accatgcgca acagttccag gcgctggttg gagaaaagca attctatcga atacgaaagc
      541 atctaggctg gtattgtaaa ggctttccac atgccgcctc acttcgcgca cagatggtgc
      601 gggtctcttc cgtcaaagaa ctccatgctc ttctcgaaga tttttacgat cggccggaag
      661 caatagggga tccaccccaa accgaatcag acgatgagcc gcgcgtgctg gtctcacgat
      721 gcagctagtg ctggcgtcga gttcgccacg gcgtcgcgaa ctcctggcac tgttgggcct
      781 tcccttcgag gtgtgcccgt cggagtttca cgaacacccc atggtcgggc tgtcacctat
      841 cgagcaggtc aagcgcttcg cgcgtgaaaa ggcgagatcc gtggcacttg tgaggccacg
      901 agccctcgtg ctcggcagcg ataccgtaat tgacctcgag agccaactgc tcgggaaacc
      961 catggatctt gtagacgcac gcgccatgct ggcacgtctg gccggccgtt ctcatcatgt
     1021 ccatactgcc gtcgccttat gcgaccgtga gcgaaacatt gaatcagtgg aggttgccac
     1081 ggctgaagtc agaatgaaag cggacctcga tcaagcttat gaacggtatc ttgcatcgga
     1141 ggaatcgtta ggcaaggccg gggcctatgc gatccaagga ctcggtggag acctcgtgga
     1201 aaggatcgcc ggtgactata cgactgtggt gggattaccg ctgaagcttg tggcgcatct
     1261 gcttcggtca gccggttatc ctattttcgt gaacgtcgaa gacctatatc ggcgcaagcc
     1321 gtatgcgaat tggaatcggt ttgcggccta attgcaaagg catgacggtt cacctacaat
     1381 gcacgtcatt catctgagtt ttctggataa tttaaacctc agcatgtcgc gctataaagg
     1441 gaaagcatga cttttcgcaa acagattgcg tcactgttag gcctgatcgg agtcatagcg
     1501 gcagggaaca gc
//
LOCUS       DASTWE010000764         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_796427, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000764 DASTWE010000000
VERSION     DASTWE010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796427"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1710
                     /locus_tag="VFT30_07910"
     CDS             331..1710
                     /locus_tag="VFT30_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5092594.1"
                     /translation="MTEEWGAVLIVDDDADMRELAYDMLKDRGHQVAMAGSGEEALRR
                     LTEEDYAVVLTDLRMKGMQGLELLTQIKRGHPDINVILMTAFGSVETAVEAMKHGASD
                     YLTKPVKKDELIRVVERVIREAALRREVNRLRKEVRKEYSFYQILGKSKSIQAVFDLI
                     RRVADSPTNVLITGESGTGKELVAKAIHYNSDRKDAPFIPVNCAAIPEQLLESELFGH
                     MRGSFTDAKMDKRGLFEEAQKGTLFLDEISELPLMLQAKILRAIQEKEIRRVGATKPI
                     SVDVRIIAATNLNLGEEVKSRRFREDLYYRLNVIELKLPPLRERREDIPLLVEAFLKK
                     CGEVRGKEVKGVGEAALAMLMDYAWPGNVRELENVIERAVTLTRGEKISSDDLPAAVQ
                     GARGDRRVLDEAAERTLPLHELEKEYIKKVLEKTGGKKYQAAHALGIDRKTLYRKLAE
                     IEGRPHSEE"
     gene            1838..>2204
                     /locus_tag="VFT30_07915"
     CDS             1838..>2204
                     /locus_tag="VFT30_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5092595.1"
                     /translation="MDAHEIGSQSVTSRKAEEQLLISRLRLESIIEAAMDAIITVDED
                     QRVVLFNHAAEQMFGCSTREAIGQPLDGFLPARFRDAHRHHIHTFGQSGATSRKMGQL
                     GTVMGLRSNGEEFPIEAAIS"
BASE COUNT          553 a          551 c          652 g          448 t
ORIGIN      
        1 gacgaagtac agatagtcga cagtctgaca gtggcgatcc ccaccgtcag cgcggtgcgc
       61 atgaacggga ttaccttgga ctatcaggag ccagggggat tcaagttcta ccacagcgat
      121 cagcagaatg atccccaact caacgttatc agcttaaact gacaccgatc tccgattaat
      181 cctctctggt cctgcctttg cacagtcgct ctatcgacat cccagacatc ggcaaactga
      241 tgaataatgc gccacataga ttgctttcct gtggcagttt gaggcagatg aaaggttcgt
      301 accattccct cattttgtgt tggggtgtga atgactgaag aatggggcgc cgtccttatc
      361 gtggatgacg atgcagatat gcgggaattg gcctatgaca tgctgaaaga ccggggtcat
      421 caggtagcca tggcaggaag tggtgaggag gctttgaggc gattgaccga agaagactat
      481 gcggtggtcc tcacggatct tcgtatgaaa ggcatgcagg gacttgaatt gctgactcag
      541 atcaaacgag gccatcccga tatcaacgtg attctgatga ccgcttttgg gtcggtggaa
      601 acggcggtcg aagccatgaa acatggggcg agcgattacc tcaccaagcc ggtcaagaaa
      661 gacgagctga tccgcgtcgt cgaacgggtg atcagggaag cggcgttgcg gcgggaggtc
      721 aatcgactca gaaaagaggt ccgcaaggaa tacagctttt accagatatt aggaaagagc
      781 aagtcgattc aggcagtgtt cgatctcatt cggcgggtgg ccgatagtcc gaccaacgtg
      841 ctcattacag gagaaagcgg aaccgggaaa gaattagtcg ccaaagccat tcactacaat
      901 agtgacagaa aagatgctcc ctttattccg gttaactgtg cggcgattcc ggagcagctc
      961 ttggagagtg aactgttcgg gcatatgaga ggctccttta ccgacgcgaa gatggacaag
     1021 cgcggactgt tcgaggaagc acagaaaggc acgctgtttc tcgatgagat cagtgaacta
     1081 ccgctcatgc ttcaggccaa aattcttcgc gcgattcagg aaaaggaaat ccggcgggtg
     1141 ggagcgacca agccgatctc ggtggatgtg cgcatcatcg cggccaccaa cctgaatctt
     1201 ggtgaggaag tgaaaagcag gcgattccgc gaagacctct attatcgtct caacgtgatc
     1261 gagctgaagt tgccgccgct ccgtgagcga cgcgaggata taccgctcct ggtagaggct
     1321 ttcctcaaga agtgcgggga ggttcgcggg aaagaggtca agggtgttgg tgaggcagcc
     1381 ctggcgatgt tgatggatta tgcctggccc ggtaacgtgc gggaattgga gaacgtcatc
     1441 gaacgagcgg tgacgctcac tcgaggcgag aagatttctt cggatgattt gcccgcggca
     1501 gtgcaagggg cccgtggcga ccggcgcgta ctggacgaag cagccgagcg aaccctgccc
     1561 ttgcatgagc ttgaaaaaga gtacatcaaa aaagttctcg aaaagaccgg cggtaaaaag
     1621 tatcaggccg cccatgccct tggcatcgac cgcaagaccc tgtatcgtaa actcgccgaa
     1681 atagaaggcc gacctcactc ggaggaatga cgacctcctc cctgaccgcc gagctggttg
     1741 atgatcgctc gaccatcgtg agcagtcgac ctgtatgtga ggcgctgtag atcattcttt
     1801 acccaactcg tttcatgcca ccgattgaga actgacgatg gacgcacacg agatcgggtc
     1861 gcagtcagtc accagccgaa aagcggaaga acagctcctg atcagccggc tcaggctgga
     1921 aagcatcatt gaagccgcga tggatgcgat catcaccgtg gatgaggacc agagagtggt
     1981 gttgtttaac cacgcggcgg agcaaatgtt cggctgctct actcgagaag ccatcgggca
     2041 gccgctcgac ggattcttgc cggctcgttt ccgtgacgct catcgtcacc acattcatac
     2101 attcggacaa tccggcgcga cgagccggaa gatgggtcaa ctaggaacgg tcatgggact
     2161 ccgctcgaac ggagaagagt ttccgatcga agccgccatt tcgc
//
LOCUS       DASTWE010000765         1463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_958135, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000765 DASTWE010000000
VERSION     DASTWE010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958135"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..745
                     /locus_tag="VFT30_07920"
     CDS             137..745
                     /locus_tag="VFT30_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5092596.1"
                     /translation="MPIDKVLSRRSSANRVISAARKQFFTHGFRTVRMDDIADELGMS
                     KKTLYAHFPSKAALVQAIMQEKFSEVEADLTRLKSTNEQNVETALRNLLDCVQRHTAE
                     IQPPFVRDIGRELPELFQVIEKRRRDLIRHHFGELFEQGQKTGLIRNDIPVHLIIEIL
                     LGAVQTIMNPPKLAELGLTVETGYVSIIRIILEGALHNQRNR"
     gene            750..>1463
                     /locus_tag="VFT30_07925"
     CDS             750..>1463
                     /locus_tag="VFT30_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU5092597.1"
                     /translation="MSRNRVGAIQWLVLAYFLVIILVQSGCERPSSDRVQGYIEGEYV
                     YVASPYAGALASLSVRRGKQVNEGDPLFALEQVFEKAARDEAEKKLGQALANLEDAKK
                     GKRPSEIESLKAQLKYAQATLRLSMQEVGRQERLKSIPGAAVEMEVDRARATRDQNQQ
                     RVAQLKADLHTALLGARADQVVAAEAEVRAREAALAKAEWALAQKRQAAPKAGLVFDT
                     LYREGEWVEAGRPVIVLLPP"
BASE COUNT          360 a          385 c          408 g          310 t
ORIGIN      
        1 ccgtcactac ccgccagcac cctcgatagt ttcctagaag agtttctgct gattctgttc
       61 ccccggttga caactcacgc aggaaactta taaaactata caagtttcct tggtgcactt
      121 gatcgattcc atgaagatgc caatagataa agtcctctct cgtcgttctt cggccaatag
      181 agtcatcagc gcggccagga aacagttctt cacccacgga ttcagaacgg tccgcatgga
      241 tgatatcgcc gatgagcttg ggatgagcaa gaaaaccttg tacgcgcatt tccccagcaa
      301 ggcggcgctt gtccaagcaa tcatgcagga gaagttctcg gaagtcgaag cggacctcac
      361 tcgtttgaaa tcgaccaatg agcagaacgt cgagacggcg cttcgcaacc tgctggattg
      421 cgtgcaacga cacacagctg aaatccaacc accatttgtg cgggacatcg gacgcgagct
      481 tcccgagctg tttcaggtta ttgaaaagcg tcgtcgagac ttgatccgcc atcattttgg
      541 cgaactgttc gaacaagggc agaagaccgg cctcatcaga aacgatattc ccgttcatct
      601 catcattgaa atcctactcg gggctgtcca gacgatcatg aatcctccca agttggcgga
      661 actcggtttg acggtggaaa ccggatatgt atcgatcatt agaattattc ttgaaggggc
      721 cctacacaat cagcgtaatc ggtgaatcga tgagtagaaa tcgtgtggga gccatacagt
      781 ggctggtcct ggcatacttt ctggtgatta tcttggtcca gtcgggctgc gagcgacctt
      841 cgtctgatcg agtccagggc tacatcgaag gggaatacgt ctatgtggct tcgccctatg
      901 ccggtgcgct tgcatccttg tctgtacggc gtgggaaaca ggtcaatgaa ggtgatcccc
      961 tgttcgcatt ggaacaggtc ttcgagaagg ccgcccgcga cgaagcggag aagaaactcg
     1021 gtcaggcgct ggccaacctc gaggatgcca agaagggaaa acgtccttcc gagatcgaat
     1081 cactcaaggc gcaactgaaa tatgcgcagg ccacgctccg tctttctatg caagaagtcg
     1141 ggagacagga acgccttaag tctatccccg gtgctgcagt ggaaatggaa gtggatcgcg
     1201 ctcgtgcgac acgagaccag aatcagcaac gagtggcgca actgaaagcc gatctccaca
     1261 cagcgctctt aggggctcgt gcggatcaag tcgtggcagc cgaggcagag gttcgtgcaa
     1321 gagaagcggc gttggccaag gcagaatggg ctctcgcaca gaaacgacaa gcggcaccga
     1381 aggcggggct tgtcttcgac accctatacc gcgaagggga atgggtagag gccggacggc
     1441 ctgtgatcgt cttgctgccg ccc
//
LOCUS       DASTWE010000766         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_925936, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000766 DASTWE010000000
VERSION     DASTWE010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925936"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFT30_07930"
     CDS             <1..996
                     /locus_tag="VFT30_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092598.1"
                     /translation="QKRIVMFVPGLIAFVVYRLVKHLVPIADPFVLLTVSSLVATVTS
                     VLAYRVGRSAPWSVIVRQDGARLLSWLAGWIGAVYGIQLSLLVLTLLWIMNYDYLQHP
                     DGPAMMAIIISCTAVARDAFEIGYVRKLSVLGRPFLTFPGGEQFRELVQRRVWQLGPW
                     VLAGLGIGAIASLSGVMIVDIQGAALAQLLSVTLLGGGLALCAYFGGLYPSVSWVRTL
                     RQTAPGELLKYWWWPGMAFASTYYLVTMGLLLFVMRQPTITLGSAAVMGALVTTMMVL
                     YGYYLGNRRHVEDEQAPQLSSGMLRCPFVMGILGKPAGADGTAAELVLGKTGTKG"
     gene            1019..2875
                     /locus_tag="VFT30_07935"
     CDS             1019..2875
                     /locus_tag="VFT30_07935"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU5092599.1"
                     /translation="MKRSAINVAICIAIVAISIGCVWNGADPSFAESSTDMYYGTEGT
                     PQGPAAPTPHETVYPRIGSLDSRLLVWFVTQQHTYFGGFVLALPLFCALLEFLGLITS
                     KPALALRYDGLARDLATVAVLALSVSALIGSLMLTMFITLYPSFMKYMGGTFKGFMPA
                     YAAVFVGEALLLIVYYYGWNRMTDRGMKWIHAAIGILTNIVGTALLMMANAWSAFMMS
                     PAGVDAQGRFLGNAWHLLHSALWNPLNVHRFLADIMSGGAVVLAYACYRFFTSKTDEE
                     RAYFDWVGYIFLFVTVCALIPMPIAGYWLMKSVFVFRQTMGVTMMGGLLTWLFVVQAL
                     LIGVLFLGVNYYLWQSMARIKGGERYQPYYQLLLGVLMLALFIWLTPHTLLMSASEVK
                     AMGGAQHPVIGNYGVMSAKNGAVNILILVTALSFLYYRRANRTMTISWAKTGNIVITM
                     LYLVGALNIIWLSIYGFYLPAKIRVGLSAPQAVTTLTVIIVGVVINRLMLRGAVVHGP
                     IQWGKIPLRGMVGLFGLAASFSWVMGLMGYIRSSGRLSWHVSELMADVSPWAFTPDLQ
                     YATKMVTLNMVVFWAAVLALFWMCERGRQPVMDEEFRDKAAPLLAPVPSQET"
     gene            2893..>3221
                     /locus_tag="VFT30_07940"
     CDS             2893..>3221
                     /locus_tag="VFT30_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092600.1"
                     /translation="MTRRVLLCTAAILGILVGVFAWQGSVVAQGQVLVLEDFQAKETD
                     GFPSLWDHENQRSQSKGREAYKVQSENGTNFLSAKDAGQRIKKKKIDWDPKAYPVLTW
                     RWRLNKAT"
BASE COUNT          599 a          774 c          993 g          855 t
ORIGIN      
        1 cagaaacgga tcgtgatgtt tgtccccgga ctgatcgcgt tcgtggtgta tcgtctcgtc
       61 aagcatctcg ttccgatcgc agatcccttt gtactgttga cggtcagtag tttggtggca
      121 acggtcacct cggtcctggc ctatcgtgtt ggtcggtccg cgccatggtc cgtcattgtc
      181 cgccaagatg gagcgcgtct cctcagttgg cttgccggct ggattggtgc cgtctatggc
      241 attcagctct cgttgttggt gctgaccctg ttgtggatca tgaattacga ttatctccaa
      301 catcccgatg ggcctgccat gatggcgatc atcatctctt gtaccgcggt cgctcgcgat
      361 gcatttgaaa tcggatatgt gcgcaagctc tcagtgttgg gacgaccatt tcttacgttc
      421 cctggtggcg agcagtttcg tgagttggtg cagcgcaggg tttggcaact gggaccctgg
      481 gttctagcgg gtcttgggat cggtgctatt gcatcgttgt cgggggtgat gatcgtggat
      541 attcagggag ccgcgttggc tcaattgttg agcgtgacgc tcctcggcgg tggcctggcc
      601 ctctgtgcct attttggtgg gctctatcca tcagtgtcgt gggttcgcac tctccggcag
      661 accgcaccgg gggaactgct gaaatattgg tggtggcctg ggatggcctt tgcttcgacg
      721 tattatctgg tgacgatggg attgctattg tttgtcatga ggcagccgac cattacgttg
      781 ggttcagccg ctgtgatggg tgccctggtc acgacaatga tggtgttgta tggctattat
      841 ctgggtaatc gacggcacgt tgaagacgaa caagcaccac aactgtcgag cggaatgttg
      901 cgctgcccat ttgtgatggg gattcttgga aagccggccg gtgctgacgg tacggctgcg
      961 gagttggtac tcggcaagac gggaacgaag gggtagatat atgggccaag gattcaggtt
     1021 gaagcgttct gcgatcaatg ttgcgatctg catcgcgata gtcgcgatct ccattggatg
     1081 cgtgtggaac ggggccgatc cgtcatttgc ggaatcgtcg acggatatgt actacggcac
     1141 tgagggaacc ccgcagggac cggcggctcc cactcctcat gaaacggtct atccgcgcat
     1201 cggctcgtta gacagtcgat tgctggtgtg gtttgtgacg cagcaacaca cctatttcgg
     1261 aggattcgtt ctcgccctgc cgttattttg cgcattgctc gagttccttg ggttaataac
     1321 ctcgaagccg gccttggcgc ttcgctacga tggtctggcg agagatctcg cgacggtagc
     1381 ggtcttggca ctgtcggtct cggcgctcat cggcagcctg atgctgacga tgttcattac
     1441 gctgtatccc agtttcatga agtatatggg cggaacattc aaagggttca tgcctgccta
     1501 tgcggctgtg tttgtcggag aggcgctact actgatcgtt tattactacg gttggaatcg
     1561 gatgacggat cggggcatga agtggattca tgctgcgatc ggcattctga ccaacattgt
     1621 gggaacggcg ttattgatga tggcgaatgc gtggtctgcc ttcatgatgt cgcctgccgg
     1681 ggtggatgcg caaggacggt tcctgggaaa cgcctggcat ctgctgcact ccgcgctgtg
     1741 gaaccctctg aacgtccatc gcttccttgc agatatcatg tctggcgggg cagtggtgct
     1801 ggcctatgcc tgttatcggt tctttaccag taaaaccgac gaagagcggg cctatttcga
     1861 ttgggtggga tatatcttcc tgtttgtcac agtctgcgcg ctcatcccca tgcctattgc
     1921 cggctattgg ctgatgaagt cggtgttcgt gtttcgccag accatgggtg tgacgatgat
     1981 gggcggattg ctcacctggc tgtttgtggt tcaggccctc ttgatcggtg tcctgttctt
     2041 aggagtcaac tactacctct ggcagagcat ggctcgaatc aaagggggcg agcgctatca
     2101 gccctactat cagctcttat tgggtgtatt aatgctggcg ctcttcattt ggctgacacc
     2161 gcacaccttg ttgatgtctg cgagcgaagt gaaggcgatg ggaggcgcgc agcatcccgt
     2221 gatcgggaac tatggtgtca tgtcggccaa gaatggagcg gtcaacatcc tgatcctcgt
     2281 cactgcgctc agctttttgt actatcggcg agcgaatcga accatgacca tttcgtgggc
     2341 caagaccgga aacatcgtga tcacgatgct ctatctggtc ggtgcgctga acatcatctg
     2401 gctgtccatt tacgggttct atttgccggc aaagattcgc gtcggtcttt cagctccgca
     2461 agccgtcaca acgctgacgg tgatcatagt tggtgtggtg atcaaccgtc tcatgcttag
     2521 aggagcggtg gtgcatggcc cgattcagtg gggaaaaatt ccgctgcggg gcatggtcgg
     2581 attgtttggg ttggccgcgt cctttagttg ggtgatgggc ctgatgggct acattcgctc
     2641 ctcgggacga ttgtcgtggc atgtcagtga gttgatggct gatgtctcac catgggcctt
     2701 caccccggat cttcaatatg ccacgaagat ggtgacgctg aacatggtgg tattttgggc
     2761 ggctgtcctc gcgctgttct ggatgtgcga acggggacgg cagcctgtga tggatgagga
     2821 atttcgtgac aaagcggcgc ccttgcttgc gccggttccg tcgcaagaaa cataagaaag
     2881 ggaatgaagg atatgacgcg tcgggttctg ctttgtaccg cagccattct cggcattcta
     2941 gtcggtgttt ttgcctggca aggttccgtt gtcgcccaag ggcaagtcct ggtgctggaa
     3001 gattttcaag cgaaggaaac ggatgggttt ccttctctgt gggatcacga aaatcagcgc
     3061 agtcaatcga aaggtcgcga agcctataag gttcaatcgg agaacggaac gaattttctg
     3121 tcggcgaaag atgccgggca gcgaatcaag aaaaagaaaa tcgattggga tcccaaggcc
     3181 tatcctgttc tgacttggcg atggcgtctc aataaagcga c
//
LOCUS       DASTWE010000767         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1346118, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000767 DASTWE010000000
VERSION     DASTWE010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346118"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..>1089
                     /locus_tag="VFT30_07945"
     CDS             27..>1089
                     /locus_tag="VFT30_07945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5092601.1"
                     /translation="MLLSDNSIRWVHSRSFPIKDSTGAQKVVGIMDDITNRKHADLRL
                     LHLAHYDALTSLPNRTLFNESLRKLIKQAEMNHRMISVLFLDIDDFKNINDTLGHALG
                     DELLCQFSLRLSECLRITDMVARLGGDEFGCILVTTDSSGDAGVVASKIRETLRQPFN
                     LGGHQATVTASMGISVYPTDSLDADALVKNGDTAMYRSKEAGKDTYRFFTAEMNMRAM
                     EKLDQENALRTALDQNEFVLHYQPKVDLSNGRITGLEALIRWKRPGHGFIGPLEFIPV
                     LEQTGLINRVGAWVIESACKQIAEWRRLGMGEIPVSVNVSGRQFSQSTLNHDVIRATR
                     ENGVSPELLEFELQTERALR"
BASE COUNT          270 a          258 c          322 g          239 t
ORIGIN      
        1 aggagggggt ggatcacgat tatcggttgc tgctttcgga taacagcatc cgctgggtgc
       61 actcccgcag ctttccgatc aaagactcga cgggcgccca gaaagtggtg gggatcatgg
      121 acgacatcac caatcgcaag cacgctgacc tgcgcttgtt acatctcgcc cattacgatg
      181 cactgaccag tcttcccaac cggacactgt tcaacgagtc gctgcgaaaa cttatcaaac
      241 aggctgaaat gaatcaccgc atgatttcgg tgctgtttct cgatattgat gacttcaaga
      301 atataaacga cacactgggg cacgcgcttg gcgatgagtt actgtgtcag ttcagtcttc
      361 gcttgtcgga atgcttgcgt atcacggaca tggtcgcccg cttgggcggt gacgagttcg
      421 gatgcattct ggtcactacc gatagctccg gtgatgcagg agtggtggcg agcaagatca
      481 gagaaacgct gcgtcagccc ttcaatttgg ggggacatca ggcaacggtt acagcgagta
      541 tggggatcag cgtttaccca acagactcgc tcgacgcaga cgcgttggta aaaaacggcg
      601 ataccgcgat gtaccgttcg aaagaggcgg ggaaggacac ttatcgcttc ttcaccgcgg
      661 aaatgaatat gcgggccatg gagaaactgg atcaggaaaa cgccctgcga acggcgcttg
      721 atcaaaacga atttgtttta cattatcagc cgaaggtgga tctctccaat ggaagaatca
      781 ccggcctgga agccctcata agatggaagc ggccggggca tggattcatc ggaccactgg
      841 agttcatacc ggtgcttgag caaaccgggt tgatcaaccg ggtgggagct tgggtgatag
      901 agagtgcatg taagcagatt gccgaatgga ggcgtctagg aatgggcgaa attcctgtat
      961 cggtgaatgt ttcgggcagg cagttctctc aaagcacgtt gaaccacgat gtgatccgtg
     1021 ccactcggga aaatggggtg tcgccggaat tgcttgaatt tgagttacaa acagaacgcg
     1081 cgttacggg
//
LOCUS       DASTWE010000768         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1378387, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000768 DASTWE010000000
VERSION     DASTWE010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378387"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..974)
                     /locus_tag="VFT30_07950"
     CDS             complement(33..974)
                     /locus_tag="VFT30_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5092602.1"
                     /translation="MNPTDIIRAIEENIARVIKGKPRVIEMSLVALLARGHLLLEDVP
                     GVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGVSIFNRQKQAFEFMPGPIFANIV
                     LADEINRTTPKTQSSLLEAMSEAQISFDNKTYPLSQPFMVIATQNPAEYHGTFPLPES
                     QLDRFLMRLRIGYPSPEEEKKVLDRPQSLHPAEALQPLLTTQDVLLLQEQVDHVLMEE
                     SITDYLLAIVQNTRQSDLLSLGVSTRGALALSRAAKALSLVRGRTYCLPDDIKELAPI
                     VLSHRIMVARTQGLHQRSFEQAERIIQDLVDSIPVPV"
     gene            complement(1117..>1810)
                     /locus_tag="VFT30_07955"
     CDS             complement(1117..>1810)
                     /locus_tag="VFT30_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092603.1"
                     /translation="GNQDMFELEDLDQQGLLAPRYAIATCWAPATPTFAYEPTRLSTV
                     LIRNELERTKGAFHQTRLGKEQALEVRHAGRALLFPPWKFEIVGPLALLSLLARRGFL
                     CVLGSVDAQVFWELLAQLLEDSRYRPPDQPFDPTNLPKVFGEVYHALDGLPIGESIDL
                     AEVAALRRHYLRPNSSPFSSPLMGGSPCHFRYVRISRGATFLGAPASSTARKFVSMTE
                     EVPPWLVTLVPA"
BASE COUNT          418 a          470 c          534 g          388 t
ORIGIN      
        1 tccacgccgg ggactcagac ttcgtggatg atctagacgg ggacgggaat cgagtcgacc
       61 agatcctgaa tgattcgctc ggcttgctca aaacttcgtt gatgcaaccc ctgcgtgcgg
      121 gccaccataa tgcggtgaga caacacgatg ggggccagct ccttaatatc gtccggcaga
      181 caataggtcc gaccacggac gagcgacaac gctttggcag cacgactcaa cgccaacgct
      241 cctcgagtac tcacccccaa tgacagcagg tccgactgcc tggtgttttg gacaatggcc
      301 aagagatagt ccgtaatgct ttcctccatg agcacgtgat cgacctgttc ctggagcaac
      361 aggacatcct gagtcgtcag caacggctgg agcgcttcag ccgggtggag tgattgcggt
      421 cggtcgagga cctttttttc ttcttccggc gaggggtacc cgatgcgaag ccgcatcaag
      481 aagcgatcca actgcgactc cggaagggga aacgtgccat ggtattcggc ggggttctgt
      541 gtcgcaatga ccatgaaggg ctgacttaag ggataggtct tgttgtcgaa cgagatctgc
      601 gcttcgctca tcgcttccaa aagactactt tgtgtcttcg gtgtcgttcg attgatttca
      661 tccgcgagca cgatgttagc gaaaatcgga cccggcataa attcaaaggc ctgtttctgg
      721 cgattgaata tcgagactcc cacgatatcg gacggaagca gatcgctcgt aaactgaatg
      781 cgtttgaagg agcaatcgag cgatcgcgca agactatgcg cgagtgtcgt ttttccgact
      841 cctggtacgt cctcaaggag gagatgtccg cgcgccaaga gggcgacgag gctcatttcg
      901 atcacacgcg gtttgccctt gatgactcgc gcaatattct cttcgatggc tctaatgatg
      961 tccgttggat tcatgttggg gctaatgtgg gagatcgctg tagaaataac gtggtgatcg
     1021 gacacggtcg aagtctaaca aagggtctga aagcggtcaa ttattcaagc ttttttcgcc
     1081 tctagggtct gacaagccac agcttgggcc agaagcttat gcaggaacca gtgtgacgag
     1141 ccagggggga acttcttcgg tcatagaaac gaacttccgt gcggtgctgc tggctggagc
     1201 acccaggaaa gtggcgcctc ggctgatacg gacataccga aaatggcagg ggctaccacc
     1261 cattaaagga gatgagaaag gagatgagtt cggccgaaga tagtgacgcc ggagtgctgc
     1321 cacctcagcc aagtcaatcg attcgccgat cggcagaccg tcaagagcat gatagacctc
     1381 gccaaagacc ttgggcagat tggtggggtc aaagggttgg tccggcgggc gatatcgcga
     1441 atcttccagc aactgcgcca ggagttccca aaacacttgt gcatctaccg atcccaggac
     1501 acataagaat ccgcgacgag cgagcaggct caggagggca aggggtccga cgatctcaaa
     1561 cttccatggc gggaagagga gcgctcggcc ggcatgcctg acttcgagcg cttgttcttt
     1621 ccccaaacgt gtctggtgaa atgccccttt ggtacgctcc agctcgttgc ggatgaggac
     1681 cgtggagagc ctggtcggct cataggcaaa ggtcggtgtg gccggtgccc aacacgtggc
     1741 gatggcatat cgtggggcta gcaacccctg ctgatccaga tcttccagct caaacatgtc
     1801 ttgattgcca
//
LOCUS       DASTWE010000769         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_214890, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000769 DASTWE010000000
VERSION     DASTWE010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214890"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..777)
                     /locus_tag="VFT30_07960"
     CDS             complement(115..777)
                     /locus_tag="VFT30_07960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5092604.1"
                     /translation="MMDRVLEPELMDDPMQAEAYARADFVEENQGFVDRFKEYFPEFS
                     QGKVLDLGCGPADIPIRFAKLYPACQVIGIDASAPMIQLGEQAVKQAGLTDRITLRCE
                     RFEELAGARIVDAVISNSLLHHLPNPLQFWQKLRQLVKPGAPVLVMDLLRPESSEAAQ
                     AIVDQYAANEPDILRRDFYNSLLAAFTEDEIGSQLARMNLTRLLIDIPDDRHWVVGGI
                     IY"
     gene            complement(839..1318)
                     /locus_tag="VFT30_07965"
     CDS             complement(839..1318)
                     /locus_tag="VFT30_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU5092605.1"
                     /translation="MTTRPSYPHVHQINVSDGGVPKLPVWEAKVSERGLDGDRQRNLK
                     FHGGPDRAVCLYSLELIEQLQEEGHPIDAGSSGENLTLCGLDWSLVRPGVRLTIGPDI
                     QLAVTSYTTPCSHNGRWFRDEEFSRISQKLNPGWSRVYAKVLRGGVVRPGDAVHVET"
     gene            complement(1321..>1613)
                     /locus_tag="VFT30_07970"
     CDS             complement(1321..>1613)
                     /locus_tag="VFT30_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5092606.1"
                     /translation="IDIFCGTLADLLQERRRAADLVLANLDRQTILDLADNLVGSASE
                     GARIMVSGILVEQEPEIVERFSGLGLVCSERYEDEGWVAMKFLRPESCDGEV"
BASE COUNT          374 a          484 c          394 g          361 t
ORIGIN      
        1 attggcttgc gagatattcc tgctgcagga tccagcttgt gtggactggc agaccgcatc
       61 aaccggacag atcccttctc atctctattg agcatactga tagatgatcc gcactcaata
      121 aatgatcccc cccaccaccc aatgccggtc atctgggata tcgatcaaca atcgtgtaag
      181 attcatgcgg gccagctgcg aaccgatttc atcttcggtg aaagctgcaa gtagagaatt
      241 atagaagtcg cggcgtagaa tatccggctc gttcgcggcg tactggtcga caatagcctg
      301 tgccgcttcc gacgattcgg ggcgcagtag atccatcacc agcacaggcg ccccaggctt
      361 caccagctgg cgaagcttct gccagaactg taacggattc ggcagatgat ggagcaggct
      421 gttggaaatg actgcatcga caattctcgc gccagccagc tcttcaaacc gttcacatcg
      481 taatgtaata cggtcagtca gccctgcttg cttcaccgct tgttcgccta actggatcat
      541 cggagcagag gcatcgatgc cgatgacctg gcaggcagga tagagtttgg cgaatcgaat
      601 tgggatatca gcggggccgc agccgagatc caatactttc ccctgagaaa actccggaaa
      661 atattccttg aaccgatcga cgaatccttg attttcttcc acgaagtcgg cacgcgcgta
      721 ggcttccgct tgcatcggat cgtccatgag ttccggctct agaacgcgat ccatcatctc
      781 tccgttaatc gttcttcgtt aatcgttaac cgcgtttaac gcttcacgtt taacgtcttt
      841 acgtctcaac atgcactgcg tctcccggtc gcaccacgcc tccacggagt actttcgcat
      901 agacacgact ccagcctggg tttaacttct gcgaaatgcg cgagaactcc tcatctcgaa
      961 accagcgccc attgtggctg caaggcgttg tatagctcgt gaccgcgagt tggatatcgg
     1021 ggccgatggt caaccgtacg cctggccgga ccaagctcca gtccaagccg cacaaagtga
     1081 ggttttcgcc agatgacccg gcatcgatgg gatggccttc ttcttgaagt tgttcgatga
     1141 gctcaagcga atacagacag acggcacgat ccggcccacc atgaaacttc agattgcgct
     1201 gacgatcgcc atccaaaccc cgttcgctca ccttggcctc ccaaaccggc agcttcggaa
     1261 caccaccatc ggagacattg atctgatgca catgcggata ggacggcctg gtggtcatcg
     1321 tcacacctct ccgtcgcagg attccggcct aaggaatttc atcgccaccc aaccttcatc
     1381 ttcgtagcgt tcggagcaga ccaatccgag accagaaaac cgttcgacga tctccggttc
     1441 ctgctcgacc agaataccgg agaccatgat tctcgcccct tccgacgcgg agcctaccaa
     1501 gttgtcggcg aggtccaaga tcgtctgccg atcgagattg gccaacacaa gatcagcagc
     1561 cctccgtctt tcttgcagca aatcagccaa cgttccgcag aaaatgtcga tcc
//
LOCUS       DASTWE010000770         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_893985, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000770 DASTWE010000000
VERSION     DASTWE010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893985"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1315
                     /gene="argJ"
                     /locus_tag="VFT30_07975"
     CDS             110..1315
                     /gene="argJ"
                     /locus_tag="VFT30_07975"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU5092607.1"
                     /translation="MRPQHIGITAPLGFQASGIHCGIKKPDLLDLALCVSNVSGPIAG
                     VFTKNRVVAAPVVLDRHHLRFRRGRAIIVNSGNANACTGEQGLVAAKTMATAVAEQLS
                     IPAHQVFVGSTGVIGRVLPIDRITAAIPTLVACLSIPGGDQAAQAILTTDLRPKTVAR
                     QAKISGRVVTIGGMAKGSGMIHPNMATMLAYLTTDAAIAPAALQLALKSAVDQSFNCI
                     TVDGDTSTNDTVLCLANGLAKNRPIQQGTKSYRDFERLLTDAAQELALMICRDGEGVT
                     KVVTIRVHGAATTAAAKRIAGTIATSNLVKTALFGEDANWGRVMAALGRSGVAIDPGK
                     VTVRFDGIVMVQRGVGMGLEAEQKIAQVFKQKEFTVTVHLGQGQAHAHMWTTDLSYDY
                     VRINASYRS"
     gene            1746..2519
                     /gene="rpsB"
                     /locus_tag="VFT30_07980"
     CDS             1746..2519
                     /gene="rpsB"
                     /locus_tag="VFT30_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5092608.1"
                     /translation="MGVVAIKELLEAGVHFGHQTNRWNPKMKKFLFGERSGIYIIDLQ
                     QTVARMEHTYAFVRDLVAAGESVLFVGTKRQAAEILEEEAKRANMYFVNQRWLGGMLT
                     NFQTIRRSIDKMKKMETTLLNPSEHGLKKKEVLLMQKDIAKLQKYLSGIKNMRSLPGA
                     VFVLDTRVEKIAVQEAKRLDIPVIAILDSNCDPDDIAYPIPGNDDAIRSIKLITSKIA
                     DACIEGAHVKTQREEAEFQATPAGGEKKPAMRVESVPVS"
     gene            2580..3176
                     /gene="tsf"
                     /locus_tag="VFT30_07985"
     CDS             2580..3176
                     /gene="tsf"
                     /locus_tag="VFT30_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU5092609.1"
                     /translation="MAGSSQLVKELREKTGAGILDCQKALSENGNDVEKSIDYLRQKG
                     LAAAAKKAGRETNQGLIHSYIHMGGKIGVLIEVNCETDFVARNEEFKAFVNDLALQVA
                     AAKPSFVKREDVPADVAEKEKTIYEGQAKEMGKPPAAWPKIVEGKLEKFYQESCLLEQ
                     SFIKDPAVTIKDLLAQKIAKIGENMNVRRFTRYQLGEA"
     gene            3173..>3550
                     /gene="pyrH"
                     /locus_tag="VFT30_07990"
     CDS             3173..>3550
                     /gene="pyrH"
                     /locus_tag="VFT30_07990"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5092610.1"
                     /translation="MSSAKYQRLLLKVSGEMLAGEQGYGIQPSILENLAEEISSVVAL
                     EVQVAVVIGGGNIFRGIAASASGMERASADYMGMLATVLNALALQNALERIGIMTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKS"
BASE COUNT          891 a          926 c          998 g          735 t
ORIGIN      
        1 ggagccgctg gtcaagccat tcaagccatg aatctgatgc tcggcattcc tgaggacacc
       61 ggccttaccg caccaggcag ttatccctaa ccactctcct cgaactggta tgagaccaca
      121 acacatcggc attacggcac cgctggggtt tcaagcctca ggaattcatt gtggaataaa
      181 aaaaccagac ctcctcgatt tggccctctg tgtgtccaac gtcagcggac cgatcgccgg
      241 ggtgttcaca aagaatcgcg ttgttgccgc gccggtggtt ctggatcgac accatcttcg
      301 atttcgtcgc ggacgggcta ttattgtcaa cagcggcaat gcgaatgcct gcactggtga
      361 acaaggacta gtggcggcga aaacgatggc gacggccgtg gcggaacagc tttccattcc
      421 cgcacaccaa gtattcgtag gttcgaccgg tgtgattggt cgtgtgctgc cgatcgatcg
      481 tatcaccgca gctattccaa cattggtcgc atgtctcagc attccaggag gcgatcaagc
      541 ggcgcaagcg attttaacca cggacttgcg gcccaaaacg gtcgcgagac aagcgaaaat
      601 cagcggccgc gtcgtcacca tcggcggcat ggccaagggt tcgggcatga tccatccgaa
      661 tatggccacc atgcttgcct atttgacgac cgatgcagcg attgctccag ccgctctcca
      721 gttggcgttg aaatcagcag tcgatcaatc ctttaattgt attacggtgg acggtgatac
      781 cagcacgaac gacacggttc tgtgtctggc taatggcctc gccaagaacc gacctattca
      841 acaaggcacc aagtcctatc gtgacttcga acgactctta accgacgcag cacaggaatt
      901 ggcgctcatg atttgccgcg atggagaagg ggtcactaag gtcgtcacga ttcgggtgca
      961 cggagccgct acgacagcag cggcgaaacg aatcgccggc acaatcgcaa catcaaatct
     1021 ggtcaagacc gctttatttg gggaggatgc caactggggc agagtgatgg ctgccctcgg
     1081 acgatcgggc gtggcgatcg acccaggtaa agtaacggtg cgattcgatg gcattgttat
     1141 ggtacagcgt ggggtaggaa tggggctgga ggcggagcag aagatcgcgc aggtcttcaa
     1201 acaaaaggag tttacggtta ccgtccacct tgggcagggt caagctcatg ctcatatgtg
     1261 gacaacagac ctctcgtatg actacgtccg cattaacgca agctatcgat cttagcccac
     1321 attattcatg agcacttctg ctccaattgt ggattcgccg ctgcgacgag ccaacatacg
     1381 gcttcggcgg catgttggcg ggcggactcg tgcacagggc actgccgact cgccgtgtcc
     1441 gcaagcatgg ggctcattat tcttggcgcc ctggacctca cgtggcagct ccgcaatttc
     1501 gcgacgaaca gtcagaaata atgtgggcta gctcaaaaag acttgaggca gcttgaaaca
     1561 gcagcaggac tatggtagcg tcctcggtct gggtttgaca ggggggttcg tttccggtca
     1621 aagagaaacg gccccattta ataaaatcac tatcagcgtc gatacgctgc acggcttgag
     1681 aataagggaa gcgattccct cgccctgtta tggggtgctc tgcaagcttg aagggaggtg
     1741 aaggcatggg agtggtggcg atcaaggagt tgttggaagc cggcgttcat ttcgggcacc
     1801 agaccaaccg ctggaatccc aagatgaaga agtttttgtt cggtgaacga agcggcatct
     1861 atatcatcga tcttcagcaa accgtcgcgc ggatggagca tacctatgct ttcgtccgag
     1921 accttgttgc agcaggagaa tccgtcttgt tcgtcggcac gaaacggcag gctgcggaaa
     1981 ttctcgagga agaagccaag cgagccaaca tgtatttcgt caaccagcgc tggcttggtg
     2041 ggatgttgac caacttccaa accattcgac gcagcatcga taagatgaag aagatggaga
     2101 cgacgcttct gaaccccagc gagcatggtc tcaaaaagaa agaagtcctc ctcatgcaga
     2161 aggacatcgc caagctccag aaatacctgt ctggcattaa gaacatgcgc agccttccgg
     2221 gtgcggtctt tgtcctggat acgagggtcg aaaaaattgc cgttcaggaa gcgaaacgac
     2281 tcgatatccc ggtgatcgcc atcttggaca gcaactgcga cccggacgac atcgcctacc
     2341 cgattccggg aaatgacgac gctattcgct cgatcaaact gattacgtcc aagatcgccg
     2401 atgcttgtat cgagggtgcg catgtgaaaa cccagcgaga agaagcagag tttcaagcaa
     2461 ctcccgccgg cggcgaaaag aaaccagcca tgcgcgtcga aagtgttccg gtttcgtaaa
     2521 tttatacgat tgatccatca acgttcatcc tcatcgatag agcgaaggaa taacgaatca
     2581 tggcaggatc cagtcagctc gtgaaggagc ttcgggaaaa aacaggagcc ggcattctag
     2641 attgccagaa ggctctcagt gaaaacggga acgatgtcga aaaatccatc gattatttgc
     2701 ggcaaaaagg cctcgcagcg gccgccaaga aagctggccg cgaaaccaac caagggctga
     2761 ttcattccta catccatatg ggcggcaaga tcggtgtatt gatcgaggtc aactgcgaaa
     2821 ccgatttcgt cgcgcggaat gaggaattca aagctttcgt caacgatctt gcccttcagg
     2881 tggcggctgc aaagccgtcg ttcgtgaagc gcgaggatgt tccggctgat gttgccgaga
     2941 aagagaaaac aatctacgaa ggacaggcca aggaaatggg caagcctcct gccgcatggc
     3001 cgaagatcgt cgaaggcaag cttgaaaagt tctaccagga aagctgcctg ctggaacaat
     3061 cattcatcaa agacccagcc gtcaccatca aggatctact cgctcagaag atcgctaaga
     3121 tcggcgaaaa catgaatgtt cgccgattca cgcgctatca gttaggcgaa gcatgagctc
     3181 tgccaaatac caacgcctcc ttctgaaagt cagtggggag atgttggccg gtgagcaggg
     3241 ttacggcatc caaccatcca ttctggaaaa cctcgcggag gaaatttctt ccgtcgtcgc
     3301 ccttgaagtc caagtggcgg tcgtcatcgg cggaggcaat attttccgtg ggattgcggc
     3361 aagtgcgtca ggtatggaac gggcctctgc cgattacatg gggatgctgg cgacggtgct
     3421 caatgcgctg gctctccaga atgcgctgga acggatcggc atcatgaccc gtgttcaatc
     3481 tgccatcgaa atgcgccagc tcgcagaggg gtacatccgt cggagggcga tccgtcacct
     3541 tgaaaagagc
//
LOCUS       DASTWE010000771         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1120290, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000771 DASTWE010000000
VERSION     DASTWE010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120290"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFT30_07995"
     CDS             complement(<1..996)
                     /locus_tag="VFT30_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5092611.1"
                     /translation="MHTDEITQKVSDRYAKAANTGEQMCCPTSYDIGQLKTFIPEEVL
                     KISYGCGTPAGLKTVQAGETVLDIGSGGGIDCFEASRLVGPTGRVIGIDMTDTMLEIA
                     RKNAAVVSANLGYPASNVEFRKGLADAMPVDDATIDLIISNCVINLAPDKRKVFQEMY
                     RVAKPGGRFTISDIVSDQIVPQYLVHDTQKWGDCLSGALTLTDYMNGMAAAGFLGIHL
                     AKFSPWRVIDGIHFFSVTLTGYKLPPAASDSSARFATLRGPFSRLVDERGTTYRRGIP
                     QSISADDAQLLSYPPFTEHFLLTSEPVKLDHQDPRWTSVLPAQVPCTWQGDYALLA"
BASE COUNT          245 a          296 c          326 g          248 t
ORIGIN      
        1 tgcgagcaat gcataatcgc cttgccaggt gcagggaacc tgcgcgggaa gcacggatgt
       61 ccagcgggga tcttggtgat ccagtttgac aggctcgctg gtcaggagaa aatgttcggt
      121 gaatggagga tagctcagta attgtgcatc gtccgcgcta attgattgcg gaataccacg
      181 ccggtacgtc gtgcctcgct catccacaag tcggctgaat ggacctcgaa gcgtggcgaa
      241 gcgggccgaa gagtctgatg ctgcaggagg cagcttgtaa ccggtcaacg tgacggaaaa
      301 gaaatggatg ccgtcaatga cgcgccaggg tgaaaacttc gccagatgaa taccgagaaa
      361 tccggctgcc gccatgccat tcatgtagtc ggtgagcgtc aacgcgccgg ataaacaatc
      421 tccccatttc tgcgtatcgt ggacgaggta ctgtggcacg atttgatcag agacgatatc
      481 ggagatggtg aaccgtccgc cgggtttggc gacccgatac atttcctgaa acaccttccg
      541 tttgtccgga gcgagattga tcacgcaatt cgagatgatc agatcgatcg tggcgtcgtc
      601 caccggcatc gcatcggcca ggcctttgcg gaattccacg ttcgatgcgg gatagccgag
      661 attggcagac accaccgccg cattcttgcg ggcgatctcc agcatcgtat cggtcatgtc
      721 gatgccgatc acgcggcctg tggggccgac caaacgggag gcctcaaaac agtcgatgcc
      781 gccgccggac ccgatgtcca agactgtctc accggcctga accgtcttga gcccggccgg
      841 tgtgccgcag ccataagaga tcttcaggac ctcttcaggg atgaaggttt tgagttgccc
      901 tatgtcataa ctagtggggc aacacatctg ctcgcctgta ttggcggctt tcgcgtaccg
      961 gtcgctgact ttttgtgtga tttcatcagt gtgcatggcc ggcctcaagt gctggtctgg
     1021 atatctgcga atatgtgtat caatacttcg cctatcgggt caacggagcc tccccaatta
     1081 cgtagaaatg atgtagaagc atacgatcgt ttccc
//
LOCUS       DASTWE010000772         1400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1249238, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000772 DASTWE010000000
VERSION     DASTWE010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249238"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFT30_08000"
     CDS             complement(<1..564)
                     /locus_tag="VFT30_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092612.1"
                     /translation="MHFQALSIGIRVPTHHFNGTVHSVFHQACNMRLESHALLTLLPS
                     EKDNVPHGIRLNIFSQPVFQDLIGVGQSVSCRGGILRIGGSDFSVDLRTARPWHIELK
                     GLHIDLRRPAQARSWAAAWAELKTSQRKCDLSKILEVVSSPKEQSDTPVAMEFLLQLS
                     THLVTALLRATRTFQLEDARAAIRSLIG"
     gene            complement(605..>1400)
                     /locus_tag="VFT30_08005"
     CDS             complement(605..>1400)
                     /locus_tag="VFT30_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5092613.1"
                     /translation="DCIQFGSPIFTKSASENGRLNSDRLGTENMMNRSHRKRERSNLS
                     WSIVTALKDQIIHWRYPPEHRLTEAELCKEFNVSRSPVREALRTLASDGFVKKLPNRA
                     YVVRQYNIGEIEELYDLRLALELYTVERLATKGPLPQNDKVAISELKSTWTALLNGSS
                     KKSEELAKLDTLFHETLAHALGNKPLLQHLRAINERLMLFRMIDFDKSDRAESTCRQH
                     LRILKCIAAGDAQGARTAMQRNIEEGRNNVHTAIKDALAKAYSLRT"
BASE COUNT          317 a          346 c          386 g          351 t
ORIGIN      
        1 cccgatcagc gatctgatgg cggctctggc atcttccaac tgaaaagtcc tcgttgctcg
       61 caggagagcg gtgaccaagt gagtggagag ttgcagaagg aactccatag cgaccggagt
      121 gtccgattgc tccttcggcg aggaaacaac ttccaaaatc ttcgaaaggt cacatttacg
      181 ttgagaagtt ttcaattctg cccaagcagc tgcccaggaa cgggcttgag cggggcgacg
      241 gagatcaatg tgcaaccctt tgagttcgat gtgccaagga cgtgcggttc gcaagtcgac
      301 ggaaaaatcg gatccaccga tacggaggat gcctccacgg caggagaccg attgaccgac
      361 tccgatgaga tcctggaaaa caggctgaga gaagatattc aagcgaatac cgtgcgggac
      421 attgtctttc tcggagggta gaagggtcag cagcgcgtga gactcaagcc tcatgttgca
      481 ggcctggtgg aacacgctat gcacagttcc gttgaaatgg tgggtcggca cccggatgcc
      541 gatcgaaagg gcctggaaat gcatgagggc actccatttg cccggtacga tgagaaaacg
      601 ctcgttacgt tctcagcgaa tacgctttgg ccagcgcatc cttaatcgct gtgtgaacgt
      661 tgtttcgccc ctcttcgatg tttcgttgca tcgcggtccg tgctccctga gcatctccgg
      721 ctgctataca tttgaggatt ctgagatgct ggcggcacgt gctttccgct cgatccgact
      781 tgtcgaaatc gatcattcga aaaagcatca accgttcatt gatggcgcgg aggtgctgca
      841 atagaggttt atttcctaag gcatgagcga gcgtctcgtg aaatagagta tcgagcttgg
      901 caagttcttc ggatttcttg gaagatccat tcaacaatgc ggtccatgtg ctcttgagtt
      961 cactgatggc cactttatcg ttctgaggga gaggaccttt tgtcgctaag cgttcgacgg
     1021 tatagagttc caacgccaag cgaagatcgt atagctcctc gatctcaccg atgttgtact
     1081 gtctgacgac ataggcccga ttcggtaatt ttttcacgaa cccatcagat gccagcgtgc
     1141 ggagcgcttc ccgcaccggg cttcgactca cattgaattc cttgcatagt tcggcttcgg
     1201 tcaatcgatg ttcgggcggg tagcgccaat gaatgatctg gtccttgagt gcggtcacga
     1261 tcgaccaact aagattggag cgctctcgtt ttcggtggct tcgattcatc atgttttcgg
     1321 tgcccaatcg atcagaattc agccttccgt tttccgatgc cgattttgtg aaaatgggtg
     1381 atccgaattg tatacaatca
//
LOCUS       DASTWE010000773         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_473812, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000773 DASTWE010000000
VERSION     DASTWE010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473812"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFT30_08010"
     CDS             complement(<1..237)
                     /locus_tag="VFT30_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092614.1"
                     /translation="MPTEGLTRDKGAGNRSIQVQISYVELLTGHRKIGGTPGVNSSSQ
                     GILGSVCQADRIGKIVRFHDGQYRPKDLLLCQTVM"
     gene            complement(244..1140)
                     /locus_tag="VFT30_08015"
     CDS             complement(244..1140)
                     /locus_tag="VFT30_08015"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092615.1"
                     /translation="MVKLKCDMRLGLMVGLCLLLGLYPWAVVLSAEEPITISKSEEYF
                     PDTVGSRWTYRGQISEGPLQSVEEKFFTNVSTVSGTKTVNGVTVTVFHDTNPGNHGPS
                     DSFYRRDAVGIVYYGSEPGTPLEKQITPYQIFRFPLRIPSSFQQFERMGVDFGSDMDR
                     DQTDEKVDAQGWSKVIGRETITVPAGTFQDAIKVEARMNMKIHLSGSRHTVSGIDVMT
                     AWFAKGVGLVKYSERQELSAIKEDRGMVTEITEELEEYDIKAAKASLSRFESPAKGVL
                     TDDSGDHELSQIIFPTRFRTHP"
     gene            1270..2046
                     /locus_tag="VFT30_08020"
     CDS             1270..2046
                     /locus_tag="VFT30_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5092616.1"
                     /translation="MEPFISSTPSAKDRRTNPSDPPAVLITGASGGIGRAISQAFGKI
                     GWYVGVHYYRNKPAAEVTLDRVLESGGIGDSYHADIREAESVRRMIDRFSGCTSGPLT
                     MVCNAGIGQSDLLVRHSDEIWDNVIATNLTGTFHCLRAMAPSLLARGGGSIIVIGSHT
                     GFHGVTGQSAYATSKAGLIGLVRTAALEWGLQNIRVNLVLPGWQKTDLTKGLFPEGQD
                     WPDHALHRPAALDEVVGTIVHVAQLKDVSGQIWNCDSRHL"
BASE COUNT          490 a          620 c          572 g          507 t
ORIGIN      
        1 catcaccgtc tggcacagga ggagatcttt gggccggtat tggccgtcat gaaagcggac
       61 aattttgccg atgcgatccg cttggcaaac ggaaccgagt atgccttgac tggaggagtt
      121 tacgccagga gtcccgccaa tcttgcgatg gcccgtgagc agttcgacgt aggaaatctg
      181 tacttgaatc gaccgattac cggcgccctt gtctctcgtc aacccttcgg tgggcaccgc
      241 ttctcagggg tgggtgcgaa agcgggtggg gaagattatc tgactcagtt catgatcgcc
      301 agagtcatca gtgagaacac ccttcgccgg ggattcgaat cgactcagtg aggcctttgc
      361 cgctttgatg tcgtactctt caagttcttc cgtgatttcc gtgaccatgc cgcggtcctc
      421 cttgatggcg gataattcct gccgctctga atacttcacg agaccgaccc ctttcgcaaa
      481 ccaagccgtc atcacatcga ttccggagac ggtgtgacgg cttcctgaca agtggatctt
      541 catattcatc cgtgcttcaa ctttgatggc atcctgaaat gtaccggctg ggacggtaat
      601 ggtttctcga ccgatcactt tactccatcc ctgggcatcg accttctcat ctgtttgatc
      661 gcgatccata tcgctgccga aatccacccc cattcgttca aattgttgga aggaggacgg
      721 gattctcagt ggaaatctga aaatctggta gggcgtaatc tgtttctcca acggtgttcc
      781 cggctcagac ccataataca caatgccgac cgcgtcgcgt cgataaaaac tatccgaggg
      841 tccgtgattt ccaggattgg tatcatggaa caccgtcaca gtgacaccgt tcaccgtctt
      901 ggttcccgag accgtcgaca cattcgtaaa aaacttctct tcgacagatt ggagcggtcc
      961 ctcgctgatc tggccgcgat aggtccagcg gctcccgacc gtatcaggga aatactcctc
     1021 ggactttgag atcgtgatcg gctcctcggc actgagcacc acagcccatg ggtacagtcc
     1081 caagagcaag cacagtccca ccattaatcc cagtcgcata tcacatttaa gcttcaccat
     1141 cgttcctgct ccagatacga gatttgacgg tatcatagga gtcccgatag cggcaagccg
     1201 ataggtgctg tgctgggttt cgatacgtgc ttgtcttggc ccgcttctca ccgcataatt
     1261 ctctccgata tggaaccctt catctcatca acaccttctg cgaaggatcg gcggacaaac
     1321 ccgtctgatc ctccagcagt cctcattacc ggggcttcag gaggcatcgg tcgtgccatc
     1381 agtcaagcgt tcgggaaaat cggatggtac gtgggggtcc attactatcg gaataaaccg
     1441 gcggccgaag tgactctgga tcgtgtcctt gagagcggtg gaatcggcga ctcatatcat
     1501 gctgatattc gagaagctga atccgttcga cgcatgatcg acagattctc cggctgcacc
     1561 tccggcccat taaccatggt ctgcaacgcc ggaatcggac agagcgattt gctcgtgcgc
     1621 cattcagacg agatctggga taacgtcatc gccacaaatc tcacaggaac atttcactgc
     1681 ctacgagcca tggcgccttc cctgctcgct cgcggggggg gctccatcat agtgatcggg
     1741 tcccacacgg gattccacgg ggtgaccggc cagagtgcat atgcgacatc gaaggccgga
     1801 ctgatcggac tcgtcagaac cgccgcgcta gaatgggggc tgcagaatat tcgcgtgaat
     1861 ctcgtgttgc ccggctggca gaagaccgac ctgaccaagg ggctctttcc agaaggacaa
     1921 gactggcccg atcacgcact gcaccgtcca gccgctctcg acgaagtcgt cggtacgatc
     1981 gtgcatgttg cccagctcaa ggacgtatca gggcagatat ggaattgtga cagcagacat
     2041 ctctaacaag agcgtatgtc tggtggctta tagcctatag ccagacacat accgaagacg
     2101 cttgatgggt cgatccgaaa cgatacgcca tatgctatac gccataagct ctttcatatg
     2161 aaccgcggca tcttcataac gggtaccga
//
LOCUS       DASTWE010000774         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1120369, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000774 DASTWE010000000
VERSION     DASTWE010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120369"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFT30_08025"
     CDS             <1..825
                     /locus_tag="VFT30_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092617.1"
                     /translation="NLVVNARDAMPTGGKLTVETASIELDHQPKYHGHPISPGEYVRL
                     SVSDTGCGMSPEVQEHIFQPFFTTKEEGKGTGIGLSTVFGIVTESGGGLDVISESGVG
                     TRFDVFLPRVVSEIEEPTDENVPLQSAEGHETILLVEDEEDVRILIRDELRKLGYRIV
                     EARNGVEACLVATPHIGKLKLLLTDIVMPGMSGTELARHLRVIKPELKLLFISGYTDD
                     VGIGAGDPASAYLQKPFTPEALASSVRGLLDVNLTNVMKGSQQPESIEQSRSVKRS"
     gene            829..>1519
                     /locus_tag="VFT30_08030"
     CDS             829..>1519
                     /locus_tag="VFT30_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092618.1"
                     /translation="MSCRHLPNHARRVLLLSVVLLPGLCPAQDISWERLSDGVTVSVW
                     KPGDACPTVPTMLVVDMDPERTKFSVHHYAQEGLSEPPRIDEWQKRTGHHVLFNAGLF
                     RENFAYLGLLYKDGRPLGSRRHASWQGLFVAEPSASGLQKARILDLAAESFDEEQPRY
                     REAAQALMLLDLTGKIRVREGGKHAYQTIVAETGTGHILLLKSLGAVRLYDIGQCFKD
                     ALPAVRQAMAMD"
BASE COUNT          352 a          379 c          437 g          351 t
ORIGIN      
        1 aatctggtcg tgaatgcccg ggatgcgatg ccgaccggcg gcaagcttac ggtcgaaacc
       61 gcttcgatcg aactggacca ccagccgaaa taccacggcc atccgatatc gccaggtgag
      121 tacgtgaggc tttcggtatc cgacaccggc tgcggaatgt ccccagaggt acaagagcat
      181 attttccagc cattttttac gaccaaggaa gaaggaaaag gaacgggaat cgggctgtcg
      241 accgtgttcg gcatcgtcac cgaaagcggc ggaggattag atgtaatcag cgagagtgga
      301 gtggggacac gtttcgacgt ttttctccct cgcgtcgtca gcgagatcga ggagccaaca
      361 gacgagaacg taccactgca atcagcggaa ggtcatgaaa ccattctgct tgtagaggat
      421 gaagaggatg ttcgcatcct catccgggat gagcttcgta agcttggata tcgcatcgtc
      481 gaagcaagaa atggtgttga agcttgtttg gtggccactc cccatatcgg gaaactcaaa
      541 ctgttactca cggatatcgt gatgccgggg atgagtggga ctgaactggc gaggcatctt
      601 cgcgtgatca agccagaatt gaagcttctc ttcatttcag gatatacgga tgatgtcggt
      661 atcggggccg gtgatcctgc cagtgcctat ctacagaaac cttttacacc cgaagctttg
      721 gcaagttccg tgcgcgggct tttggatgtg aacctgacaa atgtgatgaa aggcagtcaa
      781 caaccggaat caattgagca gtcacggagt gttaaacgat cctgagtcat gagctgtcgt
      841 cacctgccta atcatgcccg gcgtgtcctc ctgctgagcg tcgttctact gccaggtcta
      901 tgtccagctc aagatatctc atgggagcgt ctttccgacg gcgtcacagt ttctgtatgg
      961 aagccaggcg acgcttgccc cactgtgccg accatgctgg ttgttgatat ggatccggaa
     1021 cggacgaagt tctctgttca tcattacgcc caggaaggac tttcagaacc tcccaggatc
     1081 gatgaatggc agaaacgaac gggtcatcat gtcttattca atgcggggtt gttccgtgag
     1141 aacttcgcct atctcggtct tctctataag gacgggcgtc cgttgggaag ccgacgccac
     1201 gcatcatggc aggggttgtt tgtcgctgaa ccgtccgcct cggggttgca gaaggcgcgg
     1261 atccttgatc tggcagcaga gagttttgac gaagaacaac cgcgctaccg tgaagcggca
     1321 caggccttga tgcttctgga tctgaccggt aagatccgcg tccgcgaggg cgggaaacat
     1381 gcctatcaga cgatcgttgc tgaaacggga accggacaca ttcttcttct taaaagtctc
     1441 ggagccgttc gtttatacga catcggtcag tgttttaagg atgcgcttcc cgctgttcgg
     1501 caagcgatgg caatggacg
//
LOCUS       DASTWE010000775         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1346405, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000775 DASTWE010000000
VERSION     DASTWE010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346405"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFT30_08035"
     CDS             <1..249
                     /locus_tag="VFT30_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092619.1"
                     /translation="QPSVWRCEWAGARLGDGMIASGCWEWNEPSDPYSGKLAEVRSVV
                     LASETGLDEELVASLVVPSICLGCMLPRLPRLTAMLLE"
     gene            537..857
                     /locus_tag="VFT30_08040"
     CDS             537..857
                     /locus_tag="VFT30_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092620.1"
                     /translation="MKQTTNPISVRIEWCRRQSAQARTEPEGDGWSAEADGLQDALMN
                     TDHTDNYRLCPPEVLRRYLLGFQDGTALLQTVRVQRMIHAVIPGTPQPAPRAGSDALL
                     GDDR"
BASE COUNT          225 a          240 c          287 g          253 t
ORIGIN      
        1 caaccctccg tgtggcggtg cgaatgggct ggtgctcggc ttggggacgg tatgattgcc
       61 tcgggttgtt gggaatggaa cgagccgtcg gatccttata gcggcaagct ggcggaggtc
      121 aggtccgtgg tcctcgcctc tgaaacaggt ctcgatgagg aattggtcgc ttccctcgtg
      181 gtcccctcca tttgcctggg ctgcatgctt ccacggctgc ctcgattaac tgcgatgttg
      241 ttggaatgat cgcatcttca atgacggaaa gcagaatact cctctccggc cccggattta
      301 tcctagggtt gttcctagta tctcgatgaa acagtagact ttatatcctt cacttcttca
      361 gttcatggaa tactctgcta tccacactat tttgaggtaa cagtagtatc tattgctgta
      421 agtcattata aatacgtgaa tattatgtcg gatgagaaga atgaggagat gttagtccac
      481 ggttttcttt cgtggcattc aacttgctgg catataggtt acaaaggtgg tgaaacatga
      541 aacaaacaac gaaccctatc agcgttcgaa tcgagtggtg tcgaagacaa tctgcgcagg
      601 cgcgcactga gccggagggg gacgggtgga gtgcggaagc agatggactg caagatgctc
      661 tgatgaatac tgaccacaca gacaactatc gactatgccc tcccgaggtg ctcagacgct
      721 acttgctagg ctttcaagat ggaacggcgt tgctgcagac cgtgcgagtg cagcgcatga
      781 ttcatgccgt catccccggg actccgcaac cggcgcctcg ggcgggaagt gacgctctac
      841 tgggagatga tcgatgaatt gcctacgatg caacggcctc accgttagtg acgacagctt
      901 ctcgctccag agtggttcgt catcggactt tcatgggtgg cgctgtgtca attgtggaat
      961 ggttgtcgac gatgtgatca atcagaatcg gcgagagatc tcacg
//
LOCUS       DASTWE010000776         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_796780, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000776 DASTWE010000000
VERSION     DASTWE010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796780"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFT30_08045"
     CDS             <1..499
                     /locus_tag="VFT30_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5092621.1"
                     /translation="HLPIIYGERGFGSEMITQMQVKGLMFDPYNNAYIVILRDDDQSE
                     MLPIWVGKSEASSISLAIESVAPPRPMTHDFMKAYLDAFNAKVISVVITDLNEHTYFA
                     KVHLTYADSEYTVDSRPSDAIALALRSQAPIFASESVIRKQSSEELDQWLENLKPEDF
                     GKLDS"
     gene            554..1009
                     /locus_tag="VFT30_08050"
     CDS             554..1009
                     /locus_tag="VFT30_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5092622.1"
                     /translation="MIQLTVNRVVEDSTTDTRIVVLTRADGASEHFMVWVGASEGEAI
                     RRALDTSTTPRPMSHDLIKSFGEHLGIKMERAVLTDVKSSTYYATVFLENKGVARTID
                     SRPSDAIALALRCHAPIYVTQDVWKRRSGQNLDAWLSKLETKDIGAQEV"
     gene            1053..1481
                     /gene="rplM"
                     /locus_tag="VFT30_08055"
     CDS             1053..1481
                     /gene="rplM"
                     /locus_tag="VFT30_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5092623.1"
                     /translation="MTTYFENPTHVKETWHLVNAEGKTLGRLAARVASILRGKHRPTF
                     TPNVDMGDHVVIVNAEKIQLTGDKMETKLYRRHTGYPGGLKTASAAHVFRKDPTQLLT
                     KAIKGMLPKNPLGNGMARKLRVYVGPNHPHHAQRPESISL"
     gene            1515..1907
                     /gene="rpsI"
                     /locus_tag="VFT30_08060"
     CDS             1515..1907
                     /gene="rpsI"
                     /locus_tag="VFT30_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5092624.1"
                     /translation="MAVVTQYATGRRKCAVARAWVTGTEGDITVNDKPLEKAFPRLTL
                     RQIIQLPLEMAGLMGKYTISATVYGGGPTGQAGALRHAIARALVAMTPSTRLPLKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
     gene            2011..>2601
                     /gene="argC"
                     /locus_tag="VFT30_08065"
     CDS             2011..>2601
                     /gene="argC"
                     /locus_tag="VFT30_08065"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5092625.1"
                     /translation="MSEKFRIAIAGASGYAGAELVRLAAAHPYFRITAVTSEKSAGQS
                     VSSVFPSFKGIIDHQFEALAPEALTERADAIFLALPHTKSLDPVAACIKAGKPVVDLS
                     ADYRLKDGGIYEQWYHTPHSHPHLIQDAVYGLPELHRAAIAKSKLVASPGCYPTAAVL
                     QLAPLFAKRLAQPGTIVIDAKSGVSGAGRSPALAYHF"
BASE COUNT          636 a          708 c          713 g          544 t
ORIGIN      
        1 tcacttaccc attatatatg gggagagagg atttggttcg gagatgatca cgcagatgca
       61 ggtcaagggg ctcatgtttg acccttacaa caatgcgtat atcgtcatac tccgagacga
      121 tgatcagtcg gaaatgctcc ccatctgggt cgggaaatcg gaagctagct cgatcagcct
      181 ggcgattgag agtgtcgccc ctccccgtcc catgacccac gacttcatga aggcatactt
      241 ggacgcattc aacgccaagg tcatcagtgt ggtgattacg gacctgaatg aacataccta
      301 ttttgccaaa gtgcatttga cctatgcaga ttcggagtat accgtcgatt cccgtcccag
      361 cgacgctatc gccctcgccc tacggagcca ggctcctatt tttgcgagtg agtccgtgat
      421 tcgaaagcag agctcggaag aactcgatca gtggttggaa aacctgaagc cagaggactt
      481 cgggaaattg gactcctgac gaattatttt gattgttgtt atggaagagc acgcctctca
      541 aacgaccgaa ccgttgatac agcttacggt caatcgagtt gtagaagact cgactacgga
      601 taccaggatc gtcgtgctga cccgagccga cggtgcgtcg gaacatttca tggtatgggt
      661 gggagcatcg gagggtgaag ccatcagacg tgctctggac acatcgacca cgccacgacc
      721 gatgagtcac gatctgatca agagtttcgg tgagcacctc ggcatcaaga tggagcgagc
      781 ggtcctcacg gatgtcaaga gcagcacata ctacgcgacc gtcttcctcg aaaacaaagg
      841 agtcgcgcgc accatcgatt ccagaccgag cgacgccatt gcactggctc tccgctgtca
      901 tgcgcccatt tatgtcacgc aggacgtgtg gaaacgacgc agcggccaga atctggacgc
      961 gtggttgtcg aagctggaaa cgaaggatat cggtgcccaa gaagtctaac atccctcata
     1021 atgctgacag agttgcagcg gtggagaaca agatgacgac gtatttcgag aatccgactc
     1081 atgtgaaaga gacctggcat ctggtaaacg ccgaggggaa aaccctaggc agattagcgg
     1141 cacgagtggc aagcatttta cgggggaaac atcgcccgac attcacgccg aatgtcgata
     1201 tgggtgatca tgtggtcatc gtgaatgcgg agaagattca attgaccggc gacaagatgg
     1261 aaaccaaatt gtaccggcgc catacaggat atccgggagg gttgaaaacc gcctccgccg
     1321 cgcacgtgtt tcggaaagac cccacgcagc tcctgacaaa agcgattaag ggcatgctcc
     1381 ccaaaaatcc gcttggcaat ggcatggcgc gcaaactccg cgtctatgtc ggacccaatc
     1441 atccgcatca cgctcaacgt cctgaatcta tttctctcta gagacatgtc ctcaaacaaa
     1501 aggagaccag tcgcatggca gtggtgacac aatacgcaac ggggcggaga aagtgcgctg
     1561 tcgcccgggc ctgggtgaca ggcaccgagg gcgatattac cgtcaacgat aagccgttgg
     1621 agaaggcatt tcctcgtctc accctccggc aaattatcca gctacctctt gagatggccg
     1681 gcctgatggg caaatacacg atcagcgcaa ccgtctatgg gggcggcccg accgggcaag
     1741 ccggtgctct ccgtcatgcc atcgcgcggg cgcttgtcgc gatgactcct tcaacccgcc
     1801 tccctcttaa gaaggagggg ttgctcacgc gcgattcacg cgtgaaggaa cgaaagaaat
     1861 acggacagaa aggcgcacgc aaacggttcc agtactccaa gcgctaggca caggggctga
     1921 cgatccagaa aaagggaagg ctccatgcct tccctttttt gtgtcttctt tttgtgaact
     1981 gaccgttggc cagaatagaa atggtgaccc atgagtgaga aatttcgaat cgccattgct
     2041 ggagccagtg gatatgcggg cgcagaactc gttcggcttg cggcagccca tccctatttt
     2101 aggattaccg ccgtgacgtc tgaaaagtca gcaggtcagt cggtctcatc cgtgtttcca
     2161 agcttcaagg gaatcatcga tcatcaattt gaagccttgg cgccggaagc cctgacggag
     2221 cgggccgatg ccatttttct cgcactgcct catacgaaat cactggaccc cgttgcagcc
     2281 tgtatcaagg ctggcaaacc tgtcgtcgac ctcagcgcag actatcggct gaaggacggc
     2341 gggatctatg agcagtggta tcacactcct catagccatc cacatttgat tcaagacgcc
     2401 gtgtatggtt taccagaact ccatcgagcc gcgattgcca aatccaaact ggttgcctcg
     2461 cctggatgtt atcccactgc cgcagtccta cagttggcgc ctctcttcgc caaacggctc
     2521 gcgcagccgg ggacgattgt gatcgacgcc aagtcgggcg tttcaggagc ggggcgaagt
     2581 ccggccctgg cctaccattt t
//
LOCUS       DASTWE010000777         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1249404, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000777 DASTWE010000000
VERSION     DASTWE010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249404"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFT30_08070"
     CDS             complement(<1..510)
                     /locus_tag="VFT30_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEU5092626.1"
                     /translation="MSLKRKDLLSLAPLSVDEIMLVLATADSFKEVTGREIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLANGVKSSVINAGDGWHEHPTQALLDLYTIRQRGMNFRGLRVA
                     IVGDVSHSRV"
     gene            complement(523..1089)
                     /gene="pyrR"
                     /locus_tag="VFT30_08075"
     CDS             complement(523..1089)
                     /gene="pyrR"
                     /locus_tag="VFT30_08075"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5092627.1"
                     /translation="MTTPINKPVERNDEKLIMDAGDISRAMMRIAHEILERNKGVKDL
                     ALVGIRTGGVHLAHRLAKRIHSIEGVLIPIGELDITLYRDDLALRKNQPILRKTSVPF
                     DMTNKVVVLVDDVLFTGRTIRAAMDGLMDLGRPEEIQLAVLVDRGHRQLPIKANYIGK
                     NLPTSRDEQVQVLLDEDGEDDRVVILKS"
BASE COUNT          426 a          509 c          443 g          423 t
ORIGIN      
        1 gacgcggctg tgcgacacgt caccgacgat cgccacgcgt aatccacgga agttcattcc
       61 ccgttgccgg atcgtgtaca gatcgagcaa ggcttgcgtc gggtgttcat gccatccatc
      121 gccggcgtta atgacagacg acttgacgcc gttcgccagc gtttcagcag ctccggccga
      181 agagtggcgc agcacgatga tgtccgcctg catcgcttca atattccgag ccgtgtcaag
      241 aagtgtttcc cccttcacca cgctgctgga ggagggagaa aagttgatca catcggcgct
      301 caggcgcttc gccgccaatt caaaggacgt gcgagttctg gtgctcggtt caaagaagag
      361 attcacgacg gtcttgcctc ggagggccgg cactttcttg atctcgcgcc cagtcacttc
      421 cttgaaggaa tccgccgtag ccaggacgag catgatctca tccacagaca acggggcgag
      481 actcagcaga tctttgcgct tgaggctcat gggacctccg cttcaggatt ttaagatgac
      541 cacgcggtca tcttccccat cttcgtccaa caacacctgg acctgctcat cccgagaggt
      601 cggcagattt tttccgatgt aattcgcctt gatcggcagc tgccgatggc cacgatcgac
      661 caacactgcg agctggattt cttccggacg ccccaggtcc atgagcccgt ccattgccgc
      721 tcgtatcgtt ctcccggtga acaagacatc gtcgacaagt acgacgactt tatttgtcat
      781 atcaaacggc acggaagtct ttcttaggat cggttgattt ttgcgtaagg ccagatcgtc
      841 acgatacaag gtaatatcca attcaccgat ggggatcagt acgccctcga tactgtgaat
      901 acgcttggcg agtcgatggg cgagatgcac accaccagtc cttattccca ccagcgccag
      961 atcctttacc cctttattcc gttccaaaat ctcatgcgcg atgcgcatca tggcgcggga
     1021 gatatcaccc gcatccataa tcagcttttc atcgttccgt tccaccggtt tgtttatggg
     1081 agtcgtcatg gtcagatggt tatgcagcta ggttcttgcc gaaatccggg cataaaaaaa
     1141 cctcctcacg gccgcatcgt gagaaggtcg atttatcacc ggccgaggcc ggaccgaaat
     1201 tcatgttggc tccttactca cctcactgga tgagcattaa aggttcggtc atcctactga
     1261 aacacttgaa agctgtcaag cactcgaaga cagaatcggg tggtttacga ggcgaccaac
     1321 gaactatgag acattcaaga atgtgtctgc cgacagcctc tgctataggc gatcaaatcc
     1381 tgtgtcgagc tggatgatca gcctttctta tgaatgactc ttatgaatga tttgaaaaac
     1441 tggcgctgcc ggatcgctct gcttcttacg atatcgagct gtacacgacg agcagcattc
     1501 atggcagtca atcaacctac ctaatacgcc atacgccaat ctccagaagc tggaacaacg
     1561 catcgtgcaa cgaggaggct acgtacgtta cacatctatc caagtgtcga tcacacagat
     1621 ctcatgggag ccctgtcatg gcggaaccga cagagtcccc gtcgttgatg tcatcacaaa
     1681 gtttcgatca tgccacgcaa aagatcgaat cgaacgtggt aagtcacgga ccatgggttc
     1741 gccttgccga ggcaatgcat cgatacccac atttctaaac attgaggtat aacttccctc
     1801 t
//
LOCUS       DASTWE010000778         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_85960, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000778 DASTWE010000000
VERSION     DASTWE010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85960"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFT30_08080"
     CDS             <1..298
                     /locus_tag="VFT30_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092628.1"
                     /translation="CVRYRRGARAVQHNREYVRRVLSLVFESVERVVALGFVRVAVDR
                     SGDLGHSSYALGQPLRGRHCPVILIQFWRHPHAERGHEVPPVIRWREDNVSQHE"
     gene            291..>1234
                     /locus_tag="VFT30_08085"
     CDS             291..>1234
                     /locus_tag="VFT30_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092629.1"
                     /translation="MNDSGNTGTGWQTVGSWTVPGTSNQPSTSPLLRPTSGTGSGRLF
                     SYSISDSDGGNDIVSTQMLINNGLNATNGCWMYFLPGPKQLWLRDAANTSWLGPATLG
                     VSEPLSNGLCTVSPGASSSWSTGNTLTVNVSITFAFGFGGAKRHYFLATDSAGQSTSW
                     QDVGGWQVYAPNFTPYPVSVTPSSGSGQSQTFEYVFSDGNGAIDIVSTRFLINSSSSQ
                     TNACYLLFARSTNQLSLLNDAGNAYLGPVVLGSTGSLSNSQCSVNAAGSSSSLSGNNL
                     TVNLALTFSSSFNGSKTLYAQAIDTAGANSGLYYMGSW"
BASE COUNT          238 a          358 c          361 g          277 t
ORIGIN      
        1 gtgcgtccga tatcgccgcg gtgcgcgtgc tgttcaacac aatcgcgaat acgtccgacg
       61 cgtgctatct ctcgttttcg agagcgtcga acgagttgtc gctctgggat tcgtccgggt
      121 tgcagtggat cggtccggcg atcttgggca cagcagttac gctctcgggc agccgctgcg
      181 aggtcgacat tgcccagtca tcctcatcca gttctggcga caccctcacg ctgaacgtgg
      241 ccatgaggtt cctcccgtca ttcgctggcg cgaagacaac gtttctcaac atgaatgact
      301 ccggcaacac tgggaccggt tggcagacgg ttgggagctg gacggtcccg ggcacgtcga
      361 atcaaccttc gacctcgcct ctattgcggc cgacgtccgg aaccggatcg ggccggcttt
      421 tcagctacag catctcggat tcagacggtg gaaacgacat cgtttcgact cagatgctga
      481 tcaacaacgg tctcaacgcg acgaatggct gctggatgta ctttctgcca ggaccgaaac
      541 agctgtggct gcgagatgcc gcaaatacat cgtggcttgg tcccgcgacc ttaggcgttt
      601 cggagccgtt gtcgaacgga ctgtgcacgg tgtccccggg tgcgtcgtcg tcatggagca
      661 ccggcaacac cctcacggtg aacgtctcaa tcacgttcgc gttcgggttt ggcggggcca
      721 agcgtcacta cttcctcgca acggactcag cagggcagtc gacgagttgg caggatgtgg
      781 ggggatggca ggtgtacgca ccgaatttca ccccgtatcc ggtttccgtt acaccgtcgt
      841 cggggtccgg acaaagccag acgttcgagt atgtcttctc ggatggaaat ggcgccatcg
      901 acattgtctc gacgagattt ctgatcaaca gcagctcgag tcaaaccaat gcgtgttacc
      961 tcctgttcgc tcgtagcacg aaccaactct cgctgttgaa cgatgctggc aatgcgtatc
     1021 ttgggccggt cgtgctggga tccactggaa gcttgtccaa cagccaatgc agtgtgaatg
     1081 ctgcaggctc gtcgtcctcg ttatcgggca acaacctcac ggtcaacctg gcgctcacgt
     1141 tctcgagttc gttcaacggc tccaagacac tgtatgcaca ggcgattgat accgcgggag
     1201 cgaactcggg tctctattac atgggatcct ggtt
//
LOCUS       DASTWE010000779         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1314084, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000779 DASTWE010000000
VERSION     DASTWE010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314084"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1276)
                     /gene="dprA"
                     /locus_tag="VFT30_08090"
     CDS             complement(158..1276)
                     /gene="dprA"
                     /locus_tag="VFT30_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU5092630.1"
                     /translation="MDEASLTFWLTLQAIDGVGDRTLLKLIHAFGTPNAVLSATMDDL
                     IGAGCSPELAESVRRGPESSIRRQIDRQVKIVERFKIQTVTLFDRSYPARLKTIPDPP
                     PLLYLSGSVSPKDEVAVAIVGGRRATPSGRFITEEIAKDLAGCGVTIVSGLARGIDAA
                     AHRGALTGKGRTIAVLGCGIDRTYPSEHHTLRRNIESHGAVISELPIGAAPQSHHFPR
                     RNRIISGLSLGVLVGEAATDSGSLITAKLALEQGREVFAVPGSVKEEACRGSNRLIKE
                     GAKLIEGAQDILDEILPQVDARQRATLHLDRALREVHVPIGREETLVYEALSYEARSI
                     DTVIESAGLSAAEVSAALLSLELNGRIRQLPGQQYIRL"
BASE COUNT          331 a          470 c          393 g          333 t
ORIGIN      
        1 tgtgaggaac cgtcgatgac gataggaaaa tacaccggcg acaaggctta ttggctatgt
       61 tcgttcacat atcattcgtc acacgcgaag acgttcttca cgtcgatgag ccatcaattc
      121 tccctcgatg gacgctcggc gggatatctt gctgatgcta gaggcgaatg tattgttgtc
      181 cgggcaattg gcgaatccgt ccgttcaatt ccaatgaaag caacgccgct gacacctccg
      241 cagcgctcag cccagcgctc tcgatcaccg tatcaatgga tcgggcttca taggacagcg
      301 cttcgtacac caatgtctct tccctcccga tcggcacatg cacttccctc aacgcgcggt
      361 cgagatgcag cgtggcccgt tgccgggcat cgacttgcgg aagaatctca tcgagaatat
      421 cttgggctcc ttcgatcagt ttcgctccct ccttgatcag gcgattcgat ccgcgacagg
      481 cttcttcctt gacggaaccg ggtacggcga acacctctcg accttgctcc aacgccagtt
      541 tcgctgtgat caacgagccg ctgtctgttg cggcttcgcc gaccagcacg cccaaggaaa
      601 gcccgctgat aatccgattc cttcgcggga agtgatgact ttgaggagca gcgccgatcg
      661 gcagctccga gatcacggcg ccgtgcgatt cgatattccg ccggagtgtg tgatgttccg
      721 agggatatgt ccgatcgata ccgcaaccga ggacggcaat cgttcgccct ttacccgtca
      781 gcgctcctcg gtgcgccgct gcatcgattc ctcgagccag accactcacg atcgtcaccc
      841 cacatcccgc cagatccttc gcaatctcct cggtgatgaa cctaccggat ggagtcgccc
      901 gtcgtccacc aacaatcgcc acggcaacct cgtccttcgg cgatacgctt ccgctcagat
      961 acaacaacgg cggcggatct ggaatggttt tgagtcgggc tggataggat cgatcaaaca
     1021 aggtgactgt ttggatcttg aagcgttcta cgatcttcac ctggcgatca atctggcgtc
     1081 ggatactcga ctcaggcccc cgccgcacgg attcggccaa ttcaggactg caaccggcac
     1141 cgatcaagtc gtccatcgtt gcactgagca ccgcattggg agttccaaac gcgtgaatca
     1201 acttgagaag ggtgcgatcg ccgacaccgt caatggcttg gagcgtgagc cagaacgtca
     1261 aggatgcttc atccatgatc actctcgatc atcggcaggg aattcatttc cacagcagga
     1321 gagtccggca ggcagaatat ctcctcaaca aaaaccggcg gcgtgaaggt cctcgactag
     1381 aacacgacca gatcgtactg ctcaagatgc aactgactgt ccggcgtgac gatgattttc
     1441 gctgaacagt cccgctccaa tacttccact ccgtggcgct cttcgtcttg cagcaattca
     1501 gccaccgtcg gatgagctcc cacgata
//
LOCUS       DASTWE010000780         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_667856, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000780 DASTWE010000000
VERSION     DASTWE010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667856"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFT30_08095"
     CDS             <1..511
                     /locus_tag="VFT30_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092631.1"
                     /translation="AMAAVRFQRSRRLRERFGPEYERLVDEAGDKRKAESELQARLAH
                     VEALDIHPLMADEIDRFSLEWQSTQAEFVDEPLASLQKADRLIREVMKTRGYPVEDFE
                     QRAADISVDYPDLVTEYRGLHMIARKQADDEVSTEEMRQAMVHGRALFEELVRPETPE
                     TTATQKEKI"
     gene            511..975
                     /locus_tag="VFT30_08100"
     CDS             511..975
                     /locus_tag="VFT30_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092632.1"
                     /translation="MDENREKQLDIDIEDTTRAEMENEQIEQRERNESVEQVQTRAEK
                     RTQAGASETQTPAASTNDELVPLFEDEAAQKFRSRWQVIQGKFVDDPRDSVKQADDLV
                     ADIIKSVTMSFADRRIGLEKQWNGGENISTEDMRLALKRYRSFFERLLTLDS"
BASE COUNT          270 a          265 c          305 g          192 t
ORIGIN      
        1 tgcgatggcc gctgtgcgtt tccagcgcag ccggcgtctt cgagaacgct ttggtcctga
       61 atatgaacgg ttggtcgatg aagccgggga taaacgcaag gccgaatccg agctgcaggc
      121 gcgtctggcg catgtggagg ccttggacat ccatccgctt atggcagacg aaatagatcg
      181 cttctcgctc gagtggcagt ccacacaggc agaattcgtc gacgaacctc tggcttctct
      241 tcaaaaggca gacaggctga tccgcgaagt gatgaagaca agaggctacc cggtcgagga
      301 tttcgagcag cgcgcggcgg atatctctgt ggattatccc gacctggtga cggagtatcg
      361 cgggttgcac atgattgcgc gcaagcaggc ggatgatgag gtcagtaccg aggaaatgcg
      421 ccaggcaatg gtccacggac gtgccctgtt cgaagaactt gtgaggccgg agacgcccga
      481 gacaactgcg actcagaagg agaagatcta atggatgaaa acagagaaaa acaactggac
      541 atcgatatag aggatacaac tcgcgctgaa atggagaacg aacagatcga gcaacgcgaa
      601 cggaacgaaa gcgtcgaaca agtccagact agagcagaaa aaagaacgca ggcaggagcc
      661 tctgaaaccc agactcccgc cgcctccacg aatgatgaac ttgtgccttt attcgaagac
      721 gaggcagccc agaaattccg ttcgcgctgg caggtgatcc agggaaaatt tgtagacgac
      781 ccgcgcgatt cggtcaagca ggcggatgat ctcgtcgccg atatcatcaa gagcgtgacg
      841 atgagctttg ccgaccggcg cattgggctg gaaaaacaat ggaatggcgg agagaacatc
      901 tccactgaag acatgcgcct cgcgctgaag cgctatcgct ccttctttga acgcctgctc
      961 actctggatt cctgattccg gcaggatttt gaaggaattg agagtccctc tgtagactct
     1021 gacagcccct cc
//
LOCUS       DASTWE010000781         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_894317, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000781 DASTWE010000000
VERSION     DASTWE010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894317"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFT30_08105"
     CDS             <1..351
                     /locus_tag="VFT30_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU5092633.1"
                     /translation="DTSLFLELHRLPELFCGFSRRAGESPTLYPTACSPQAWAAGAGF
                     LLLQACLGLRIDAPQRLVSFVRPVLPPFLEQVEIRNLSVGTARLDLVLNRHENEVALR
                     VVRRTGEVEVVLTI"
     gene            complement(444..>1124)
                     /locus_tag="VFT30_08110"
     CDS             complement(444..>1124)
                     /locus_tag="VFT30_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5092634.1"
                     /translation="RVALSQAATAPRTTIRTIMAQGSLAKIIAREARRLRADLIIMGS
                     RGLSDIQGFLLGSISRQVASIAPCSVWVVKQSTHKLLHVALAVDDSAPSRAAARFLRS
                     SILPETATVTILSAVESPVTDLAARYLSESQLAELTEPVMERTRAFINGLRNDFIKEG
                     FPVVTEVQMGHVIDTVVKYVETNRDELLVIGARNLTKSERLQLGSVSESLLRHAPCSV
                     LIVRGART"
BASE COUNT          224 a          328 c          324 g          248 t
ORIGIN      
        1 gataccagtc tctttctcga actccatcgg ctgccggaat tgttctgcgg attttctcgg
       61 cgtgccgggg aaagcccaac gctctatccg acggcttgtt ctccccaagc ttgggcggcc
      121 ggtgccggct ttctgctgct ccaagcctgt ctgggcttac gaattgacgc tcctcagcgt
      181 ctggtgagct tcgtccgtcc tgtgctgccg ccgttccttg agcaagtcga gattcgaaat
      241 ctctccgtcg gaaccgcacg tcttgatctc gtgctcaacc gccatgagaa cgaagtagcg
      301 ctgagggttg tccgccgaac cggcgaggtg gaggttgtgc tcaccatcta gccaacaccc
      361 ggtaggttcg gagctttccc actcgtctct caccgcttcg atcggattca gattcgaagc
      421 catcatccac actttgcgag aattcacgtt cgggcgcctc ggacaatcag caccgagcaa
      481 ggagcatgtc ggagtaggct ctcagatacg cttccgagtt ggagtcgctc actcttcgtc
      541 aaattacgag ctccgatcac caggagctcg tcacgattgg tttccacgta tttgacgacg
      601 gtatcgatga cgtggcccat ctgcacctcc gtcacgacag gaaaaccctc cttgatgaag
      661 tcatttcgca acccgttgat gaacgccctg gtccgttcca taaccggctc ggtcaattca
      721 gccagttgcg attcggaaag gtagcgcgcg gccaagtccg tgacggggct ctctactgca
      781 gaaaggatcg tgacggtggc agtctccgga agaatggaag agcgaaggaa acgcgcagcg
      841 gcacgcgacg gtgcggaatc gtcgactgcg agggcgacgt ggagaagttt gtgagtcgat
      901 tgtttcacga cccataccga acaaggagcg atcgaggcga cttggcgcga aatgctcccc
      961 agtaagaacc cttgaatgtc gctcagtccg cgcgagccca tgataatcaa gtctgccctc
     1021 aagcgacgcg cctctctggc gatgatcttc gcaagtgagc cctgagccat gatggtccgg
     1081 attgttgtgc gaggtgcagt ggcggcttgg ctgagggcca cgcg
//
LOCUS       DASTWE010000782         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_474100, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000782 DASTWE010000000
VERSION     DASTWE010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474100"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..>1062
                     /locus_tag="VFT30_08115"
     CDS             176..>1062
                     /locus_tag="VFT30_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5092635.1"
                     /translation="MELDDRAVPLTRGQLDIWLAQASRPGTEWQLGLFVKIKGLVNRQ
                     VLGRAICQALHEADSTRAAFFEANGRVFQTVIDYPEIELDFFDLSRSDHPVQEAQMIA
                     SSIQHTPMPMTGPLFKFALFQTRADECYWFTCCHHLITDGTGMGLIGRRIAAIYTAMV
                     SGAPIPAAFFGKLQDLVCTELEYDTSGDYSHDQAYWEKTLPKDELVFHSPQAKRGIES
                     SGPSSPAQLDSWVIARTKELSKALGIRRSSVLAAACALLVHGFSTEGSQVVFDFPVGR
                     RVHPALKLLPGMTAGVVPLV"
BASE COUNT          216 a          299 c          313 g          234 t
ORIGIN      
        1 ctcatcggtt gccggcatcg tggacggtga gctcgagcat ccggtccacc tcttggaaca
       61 tcgtttttct ccgaacgtcg ctgacgtaac ggttaatata cgaccgaagc gcgtgagcga
      121 aggctagcaa aaggttgcgc gttgatcgac aagccgtcaa cattcgggaa atctgatgga
      181 attggatgat cgcgcagtcc cgctgacgcg ggggcaattg gatatatggc ttgcccaagc
      241 cagccgccct gggaccgaat ggcagctcgg gctgttcgtg aaaatcaagg gtttggtaaa
      301 ccgtcaggtg ttggggaggg cgatctgcca agcgctgcac gaggctgatt cgactagagc
      361 cgcgttcttc gaggccaatg gtcgagtttt ccaaacggta atcgactatc ccgaaatcga
      421 attggacttc ttcgatctga gccgctcgga ccaccctgtg caggaagctc aaatgatagc
      481 gtcgtcgatc cagcacacgc cgatgccgat gactggtccc ctgttcaagt ttgcgttgtt
      541 ccagacgcgg gccgatgaat gctactggtt cacctgctgc caccatctca tcacagatgg
      601 aacgggcatg gggctgatag gtcgtcgaat cgccgcaatt tacaccgcaa tggtttccgg
      661 cgcccccatc cccgctgcat tcttcggcaa actgcaggac ttggtttgca ccgagctgga
      721 atacgatacg tctggcgatt attcacacga tcaggcgtac tgggaaaaga cccttccgaa
      781 ggacgaattg gttttccact caccgcaggc caagcgcggg attgagtcgt ccggaccatc
      841 ttccccagcg cagttggact catgggtcat cgcccgcacc aaggagctgt ccaaagcgct
      901 ggggatccgc cgatcgtcgg tactggctgc ggcgtgcgct ctcttggtgc acggattcag
      961 caccgagggt tcgcaggtgg tattcgactt tccggtcggc cgacgcgtgc atccagcatt
     1021 gaagttgctt cccggaatga ccgccggagt tgtgccgctc gt
//
LOCUS       DASTWE010000783         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1605274, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000783 DASTWE010000000
VERSION     DASTWE010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605274"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFT30_08120"
     CDS             complement(<1..886)
                     /locus_tag="VFT30_08120"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEU5092636.1"
                     /translation="MPWVPQSTLFRKPGGREAFVIEDNPKIRAFIAKVLLSSGFSAHQ
                     FSQISELEAALTVLKPDVIVLDLSLGDSDAVEVMRSLAAVRYHGKVLLMSGHGLGTID
                     DVRLIGSRLGLAMLPPLHKPFRLEDLKARLSGVSPIEIRTNGSLLDLGLQNNWLELWY
                     QPKIDLKSMLVCGAEALIRLRHPKDGILDPAQFLPPAGDPLYKPLSDFVVRRALADWR
                     CLAAEQITIRLAINIPAPILQCTEFVVNLRRHIPQQTEFPGLIVEITENEAIADPDVA
                     REVAVQLKLYNILLAIDDF"
BASE COUNT          224 a          267 c          299 g          226 t
ORIGIN      
        1 cgaaatcgtc gatggccaaa aggatattat aaagcttgag ctgtaccgcc acctcgcgtg
       61 caacgtctgg atcggcgatc gcctcgttct cagtgatctc gacaatcaac cccggaaact
      121 ctgtttgttg cggaatatgt cggcgcagat tgacaacgaa ctcagtgcac tgaaggatcg
      181 gggcgggaat attgatcgcg agtcttattg tgatctgttc agctgcgagg catcgccagt
      241 ccgccagcgc tcgccgtacg acaaaatccg acaatggctt gtagagcggg tcgccagcag
      301 gtggcaggaa ttgtgcgggg tccaggatgc cgtccttcgg atgcctgaga cgaatgagcg
      361 cctccgcgcc acaaacgagc attgatttta gatcgatctt tggctgatac catagctcca
      421 gccaattgtt ctgcagacca aggtcgagta gggacccgtt cgtcctgatc tcgatcggag
      481 acactccaga cagccttgct ttgaggtctt cgaggcggaa cggcttatga agagggggta
      541 gcatcgcaag gccaagccga ctgccaatca gccgcacgtc gtcaatggtt cctaaaccat
      601 gaccgctcat tagcaggact tttccgtggt accgcaccgc ggcgagactg cgcataacct
      661 cgacggcgtc ggaatctccg agtgataggt ccagaacgat gacatccggt ttcaggactg
      721 tcagggctgc ttcgagctcg gaaatctggc tgaattggtg agctgaaaag ccggagctta
      781 ggaggacttt tgctataaac gcccggatct tcggattatc ctcgatcaca aaagcctccc
      841 ggcctcctgg ctttcggaat agggtggatt gcggcaccca aggcatggaa ctgtccttag
      901 ttctcggtgg aggctaaccg tgaggggcgt cggtttccca agagcggccg atggtgcctg
      961 cgactggttt caatgaggct aacgcgtgtc ccttcggact gttgggacta atggga
//
LOCUS       DASTWE010000784         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1055821, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000784 DASTWE010000000
VERSION     DASTWE010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055821"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1126)
                     /locus_tag="VFT30_08125"
     CDS             complement(56..1126)
                     /locus_tag="VFT30_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEU5092637.1"
                     /translation="MSIQVEHVTKRFGTFTVLNDVNLQVESGELVALLGPSGCGKTTL
                     LRILAGLERPDQGHIFLHGAEVTDQRITERRVGFVFQHYALFRHMTVAENVGFGLSVL
                     PRAQRPSTARIQEKVRELLTLVQLQAMADRYPSQLSGGQRQRVALARALAVEPKLLLL
                     DEPFGALDAKVRKELRRWLRRLHDELHITGILVTHDQEEALEVADRIVVMHQGNVEQI
                     GTPDDVYEHPASPFVYQFLGDVNVFHGRAVRNFADLPPLPDGDLVAPVDVVDEHEVTF
                     VRPHDLEVHRQRTNPSQLEATVRYVLTAGSRVWIELIAHDPTQTIEVELTKDRYRELN
                     LHEGEIVFVHPRRRQVFKMPQS"
BASE COUNT          278 a          348 c          324 g          284 t
ORIGIN      
        1 tagttgagtt cgcgccgtga ctgctagctt taaagaaaga tgccatcatc gcgtgctaag
       61 actgtggcat tttgaaaacc tgacggcgtc taggatgcac aaacacaatc tcgccctcat
      121 gaagattcaa ctcgcgatat cgatccttgg tcagttcgac ttcgatagtc tgcgtcgggt
      181 cgtgtgcaat caactcgatc cacacacgcg atccggctgt gagaacatac cgcacagtag
      241 cctcaagctg tgatggattc gtgcgctgtc gatggacttc caggtcatga ggccgaacaa
      301 acgtgacttc gtgttcatcg accacgtcga ctggcgctac gaggtcccca tctgggagcg
      361 gcggcaggtc tgcgaagttt cgtacggcac gcccatgaaa cacattgaca tcgccaagaa
      421 attgatacac gaacggggat gcaggatgtt catacacgtc atccggcgtg ccgatctgct
      481 caacattgcc ttgatgcatc accacgatac ggtcggccac ttctaatgct tcttcttggt
      541 catgagtgac gagaatcccc gtaatgtgca actcgtcgtg aagccgtctg agccagcggc
      601 ggagctcttt acgaaccttg gcatccaagg ctccaaacgg ttcatcgagc agaaggagtt
      661 tcggttccac agccagtgct ctggccagtg caacacgttg tcgctgtcct ccagacagct
      721 ggctgggata acgatcggcc atcgcttgca actgcacaag cgtgagcagt tcacggacct
      781 tttcctggat gcgcgcggtt gaaggccgtt gggcccgggg tagcacacta agtccaaacc
      841 ccacattttc agccactgtc atatgtcgaa acagcgcgta atgctgaaag acgaacccca
      901 ctcgacgttc ggtgattcgc tgatcggtca cttctgcacc gtgcagaaag atgtgtccct
      961 gatccggtcg ttccaatccg gcgagaattc tcagcaacgt cgtcttgccg caaccggatg
     1021 gccccaggag tgcaacaagc tcgcctgact cgacctgaag attgacatcg ttcaataccg
     1081 tgaacgtgcc gaaccgtttg gtgacatgtt cgacctggat actcatgatg gactcctggc
     1141 taaataggca ccttcgcccc ttaccgctga ctcttgagct ggttgggtga ggcggttttc
     1201 cgttcaatca atgctttcgc cgagagcgtc acga
//
LOCUS       DASTWE010000785         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_667949, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000785 DASTWE010000000
VERSION     DASTWE010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667949"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..>1096
                     /gene="argH"
                     /locus_tag="VFT30_08130"
     CDS             17..>1096
                     /gene="argH"
                     /locus_tag="VFT30_08130"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5092638.1"
                     /translation="MDRGQFVFSSQDEDIHMAIERRLTELIGPLGGKLHTGRSRNDQV
                     ALDIRLYLRTQLDELHERLAGFQRVLVAKAGANRTLMMPGYTHLQQAQPVLFAHHLLA
                     YVEMLERDKGRIRDARGRLNVMPLGSGALAGTNYPVNRRYTAELLGFPAVTLNSMDAV
                     SDRDFMIEVASAFSIVMMHLSRLSEELILWASQEFQFVDLPDAFCTGSSMMPQKKNPD
                     VPELVRGKTGRVYGHLINLLTLLKALPLSYNRDLQEDKPAVFDALDTVSASLQVMTEL
                     MRRLKVNGEVLKRALQGGGLLATELADYLVMRGVPFREAHGITGRIVRAALDQGRDIT
                     DFSLEELRVFCGRIDKSVFSRLTTAA"
BASE COUNT          254 a          281 c          310 g          251 t
ORIGIN      
        1 atcagtcaaa cgtgagttgg atcgtggaca attcgttttc tcatctcaag acgaagatat
       61 acacatggca atcgagcggc gattgaccga attgatcggc ccgttggggg gcaaattaca
      121 cacaggccgt agtcggaatg atcaagtggc cttggatatt cggctttatt tgcgtactca
      181 attggatgaa ctccatgaac ggttagccgg attccagcgc gtgctggtcg ccaaagccgg
      241 tgcaaaccga acactcatga tgcctgggta tacccacctg caacaggctc agccggtcct
      301 gtttgcgcat catttgctgg cgtacgtaga gatgctcgaa cgtgacaaag gtcgaatacg
      361 tgacgccaga ggccgactca atgtcatgcc gctcggatcc ggcgccttgg ccggtacgaa
      421 ctatccggtg aaccggcgat atacggctga actgctgggc tttcctgccg tcaccctgaa
      481 tagcatggat gcagtttcag accgcgattt catgattgaa gtggcatcgg ccttctcgat
      541 tgtgatgatg catctgtcac ggcttagtga ggaattgatc ctatgggcgt cacaggaatt
      601 tcagttcgtc gatctgccgg atgcattctg tacaggaagc agcatgatgc cgcagaagaa
      661 gaaccccgac gtccctgagc tggttcgagg aaaaaccgga cgcgtatatg ggcacctcat
      721 caatctgctg accctgctca aggccctgcc gttaagttat aatcgagatt tgcaagaaga
      781 caaaccggcg gtctttgatg cccttgatac cgtgtcggca tccctccaag tgatgacgga
      841 attgatgcgc cgactgaaag tcaacggaga ggtcctgaag cgagcgttgc aaggaggcgg
      901 acttctcgcc acggaactgg ccgactactt ggtcatgaga ggcgtgcctt tccgcgaagc
      961 ccacgggatc accggacgaa tcgtccgagc tgcgctcgat caaggacgcg acatcacgga
     1021 tttttcactt gaggaacttc gtgtattttg tggacggatc gacaagagcg tgttttctcg
     1081 gctgactacc gcggcg
//
LOCUS       DASTWE010000786         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_344776, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000786 DASTWE010000000
VERSION     DASTWE010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344776"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..608
                     /locus_tag="VFT30_08135"
     CDS             111..608
                     /locus_tag="VFT30_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5092639.1"
                     /translation="MIESDWFVQAMQDMGINFFTGVPDSILGGIIAELMNRRLYTPAV
                     REDEAVGMAAGAYMAGKTPAVLMQNSGLGTSLNTLISLNMIYKQPCILIVSWRGQGGK
                     DAPEHLVMGEVMPQLLDTVKIPHRTLTEKTAIEDFRWVAQMFMQQQIPVALLITKGVV
                     KGLHP"
     gene            715..1296
                     /locus_tag="VFT30_08140"
     CDS             715..1296
                     /locus_tag="VFT30_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5092640.1"
                     /translation="MRPEQGTLISRAQAMAALLELLTDQPVIICNGFPSREAHKIADR
                     STHFYMIGSMGNAPAIALGVALAKPNKQVITFDGDGNVLMGMGTLATVGALKPKNFIH
                     VVFDNEVYGTTGNQPTISNVVPLEKVAKSAGYVNVERVLDRDDLVYEFKDMLKKDGPS
                     LLLIKVNEFVEDAGRVLHDPPEITRRFMKAIEG"
     gene            1460..2542
                     /locus_tag="VFT30_08145"
     CDS             1460..2542
                     /locus_tag="VFT30_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5092641.1"
                     /translation="MVLLNPGPVNVSERVRQALLKPDICHREDEFSEPLHRIQAKLLK
                     AFVPGAESEYVAVVMTGSGTAAVEAALMSSLPHGRRMLILNNGVYGERMSQIVGLNRL
                     GVSELKYEWTVRPDPQKLLLALRQHQEVHAVGMVHHETTTGLLNPVHEIAEIVDNQNR
                     VFVLDAVSALAGETIDIARSHIYMVAGTAGKCIQGFPGVSFVLVRKGFVEKMRSYPKR
                     SWYLHLTHYIDNEGRGTIPFTPAVQVYYAFDEALNELLEEGVSNRIQRYKKMATIIRE
                     RMAKLGVKALLPADRQSNTITAYHLPDGISYHALHDRLKQQGYVIYAGQGNLENKIFR
                     VANMGALSEAQFGGFLDAFEQACKSA"
     gene            2539..>2657
                     /locus_tag="VFT30_08150"
     CDS             2539..>2657
                     /locus_tag="VFT30_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5092642.1"
                     /translation="MKAVILAAGVGKRLWEVTQHRPKCLIEIGGRSLLRRYLES"
BASE COUNT          610 a          688 c          788 g          571 t
ORIGIN      
        1 atgcgagtga agtcaactct actgcggatc ttcttgagaa ccccgctggc cgtattttat
       61 aatgcgccgg ctttgggtgg gcgtgtatca aaattccagg ggaactctca gtgattgaaa
      121 gcgactggtt cgtacaagcc atgcaggaca tggggatcaa ttttttcacc ggcgtaccgg
      181 attctattct gggtgggatc attgcggaac tcatgaaccg tcgactctac acccccgcgg
      241 ttcgggagga tgaagcggtc ggtatggctg cgggcgcata catggcggga aagacacccg
      301 ccgtgctcat gcagaattca ggactcggca cctcactcaa tacgctcatt tctctgaata
      361 tgatctacaa acaaccctgc atattgattg tttcatggcg agggcaagga ggaaaggatg
      421 ctcccgaaca tttggtgatg ggagaagtga tgccgcaatt gctcgatacc gtcaaaattc
      481 cgcatcgtac tttaaccgaa aaaacggcta tcgaagattt cagatgggtg gcccagatgt
      541 tcatgcagca acagattccg gtcgcgttgc tcatcaccaa gggcgtcgtg aaggggttgc
      601 atccatgaac gtgtacgtgt catgcgtcac tcgtcgttca tcgatcgaac gatacgtgtg
      661 gtcgtcagcg ttggcgatcc ccaattggcg ggtgaagtgt gatgaggtga tgagatgagg
      721 cccgaacaag gcacattgat cagccgggcg caggcgatgg cggccttgct ggagctgctg
      781 accgatcagc ccgtcattat ctgcaacggg tttccctcgc gcgaggctca caagatcgcg
      841 gatcgttcca cccatttcta catgatcggc tccatgggga atgccccggc gattgcactc
      901 ggtgtcgcac tcgcaaagcc gaataagcag gtgattacct tcgatggcga cggaaatgta
      961 ctgatgggaa tgggcacact cgcgacggtc ggtgcattga aaccgaaaaa ctttatccac
     1021 gtcgtattcg acaatgaagt gtacggaacg accggcaacc agcccacaat ctccaacgtc
     1081 gtgccgctgg agaaagtggc gaagtcggcg ggctacgtga atgtggaacg ggtacttgat
     1141 cgcgacgatc tcgtctacga attcaaagac atgctgaaga aggatgggcc cagcctgttg
     1201 ctcatcaagg tgaacgaatt tgtggaagat gccggccgcg tcttgcacga tccgccggag
     1261 atcacccgcc ggttcatgaa agcgattgag gggtagggga cgggacgagc gtcccatgtg
     1321 ctcgccgatc gcgcaggacc cgagcaagac ggggtatcgc gatgtcgggt aaggccgggt
     1381 ggaatgaacg tggtcggtca atgcacgcaa tgataatgat aaggaagtcc atcacgctct
     1441 ctaagaaaag gaaagaagag tggttctcct gaatcccggg ccggtgaacg tttccgagcg
     1501 ggtgcggcag gcgctgctga aacccgatat ctgtcaccga gaagatgaat tttccgaacc
     1561 gcttcaccgc atccaagcca agctgctcaa ggcctttgtt cccggagctg aatcggaata
     1621 cgtcgccgtg gtgatgaccg gatcagggac ggctgccgtt gaagccgcct tgatgtcgtc
     1681 gctccctcat ggccgccgta tgctcatcct caacaacggc gtctatggtg aacggatgtc
     1741 tcaaatcgtg ggcctcaatc gcctaggggt gagcgagctg aagtatgaat ggacggttcg
     1801 gcccgatccg caaaaactgc ttcttgcctt gcgacagcat caggaagtgc atgccgtcgg
     1861 catggtgcat catgagacca ccaccggact tctcaatccg gtccacgaga tcgccgagat
     1921 cgtcgataat cagaatcggg tgttcgtgct cgatgccgtc agcgctctgg cgggcgagac
     1981 gatcgacatt gcgcggtcgc acatttatat ggtggcgggg acggcaggaa agtgtattca
     2041 ggggttcccg ggcgtctcgt tcgtgctggt gcggaagggg tttgtcgaga aaatgcgttc
     2101 gtacccgaag cgctcgtggt atctccattt gacccattac atcgacaatg aaggacgagg
     2161 cacgattccc ttcacgcccg ccgtgcaggt ctactatgcc ttcgacgaag cactcaacga
     2221 actactcgaa gaaggcgtgt ccaatcgcat ccaacggtac aagaagatgg caaccattat
     2281 ccgcgagcga atggccaagc tcggtgtgaa ggcattgctc cctgcggacc ggcaatcgaa
     2341 taccatcacg gcctatcacc tgccggatgg tatttcctac catgcgttgc atgaccgcct
     2401 gaagcagcag gggtatgtca tttatgccgg tcaaggcaac ttggaaaaca agattttccg
     2461 cgtggccaac atgggggcat tgtccgaggc gcagtttggc gggtttctcg acgccttcga
     2521 gcaagcgtgc aaatcggcat gaaggcggtc atccttgcag cgggagtggg aaaacgtctg
     2581 tgggaggtca cccaacatcg tccgaaatgc ctcatcgaga ttggcggccg gtcgctcctg
     2641 cgtcgttatt tagaatc
//
LOCUS       DASTWE010000787         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1249644, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000787 DASTWE010000000
VERSION     DASTWE010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249644"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFT30_08155"
     CDS             complement(<1..682)
                     /locus_tag="VFT30_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU5092643.1"
                     /translation="MIKIMRNAAHNYPWLLKSIMGILAIAFVITMGWWGFGEQGSGPV
                     AKVGEQSVSIDEFKRTYENMRRIYKENVKTDFKEEEFKEFVVGQLVDSRIWIIAAEEM
                     GVRVSDADLRELIMQTPVFQKNGAFDPELYKRILAANHLTPSSFESIQQQEVLANKAR
                     MIVRDSVALTPAEIAEGQSLMTRPQDSDPAKAAEAKQRVLDDMLLQKQQRALVSFQQS
                     MKAKLPITI"
     gene            935..1393
                     /locus_tag="VFT30_08160"
     CDS             935..1393
                     /locus_tag="VFT30_08160"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU5092644.1"
                     /translation="MVGEGPTSTALKARVYPKAHVLNRFIAKLIDLFIVVAAGEIAPP
                     VGFLSGLAYILIADGFAGGKSIGKRLVGLQTMRLDGRDTAGFRESIIRNLPLGGAQIA
                     YTVPYIGWLVSLAILAFESFLIIGNEQGRRLGDEVARTHVLDAGQLAVPD"
     gene            1478..2509
                     /locus_tag="VFT30_08165"
     CDS             1478..2509
                     /locus_tag="VFT30_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5092645.1"
                     /translation="MFGWFSDDLAIDLGTATTLVYVHGKGIVLNEPSVVAVEKKSEKV
                     LAVGADAKKMLGRTPGNIVAVRPMKEGVIADFEMAEQMLKHFIRKAHNRSAFVRPRII
                     IGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGSGLPITEPSGNMVVDVGGG
                     TTDIAVISLGGIVYSESVKVAGDRMDEAIMNYIKKKYNLLIGEHMAERIKFEIGSAYP
                     FEERKTMMIKGRDLISGIPRTLVIDDAEIREALQEPIGTIVNAIKIALENTPPELAGD
                     IIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELDLLRKVS
                     VMSQANNLR"
     gene            2523..>3092
                     /gene="mreC"
                     /locus_tag="VFT30_08170"
     CDS             2523..>3092
                     /gene="mreC"
                     /locus_tag="VFT30_08170"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5092646.1"
                     /translation="MRMVNLRAPYSARRVALILAVILVLGFLLLPGQLQSGFQYIGSP
                     LGWIISWPLQAVAGIHDRIADIWGRYVALQEVEEENQQLRRELDLLREQNGQLREASV
                     ATERLSALLEFKKQVLPTSVAAQVIGRDTGNWYKTIILNKGASDGLQSDQGVITPAGV
                     VGRVVKTTGSTAVVLLVTDPNNAIAGLIQR"
BASE COUNT          698 a          788 c          898 g          708 t
ORIGIN      
        1 ggatggtgat cggcagcttg gctttcatcg attgctggaa tgacacgaga gcgcgctgct
       61 gtttctgcaa cagcatgtca tccaacaccc gctgcttggc ttctgctgcc ttagcagggt
      121 ctgaatcttg tggcctggtc atgagagact gcccctcggc gatctccgcc ggggtcagcg
      181 caacggagtc tcgtacgatc atgcgggcct tattggccaa aacttcctgc tgctggatcg
      241 actcaaacga cgatggagtg agatgattcg cagcaagtat acgtttgtaa agttctgggt
      301 cgaaggcgcc atttttctga aagaccggcg tttgcatgat cagctcacgc aaatcggcat
      361 cggaaacccg tacgcccatt tcttcagccg cgatgatcca gatacgacta tcgaccagtt
      421 gtccgacgac gaattccttg aactcttcct ctttgaaatc ggtcttgacg ttttctttgt
      481 agatgcggcg catgttttca taggtacgct tgaattcatc gatggaaacg gattgctccc
      541 cgactttggc gacgggtccg ctgccttgtt ctccgaatcc ccaccatccc atcgtaatca
      601 cgaaggctat agccaagatc cccatgatgg atttgagcaa ccaaggatag ttatgagccg
      661 cattgcgcat tatcttgatc atgcaatcct cttcttgtgt gatccagcag tgtacttatg
      721 gacagaacgg aaggcaagag cgggcacggt caagcattac gcggaatgtc tacaagcgcc
      781 agtttcttgg agaacggcgc cttcgcactg tttccagtag gccgagagac ggttcggcaa
      841 ggttttgaaa agaaaaccag gaaaaaagat cggtctttgt ttgtgcaagt tgatgcaatt
      901 tgttatactt tgccactaat tagaaggaga acccttggta ggggaggggc caacctcgac
      961 ggcgctgaaa gcaagagtct atccgaaggc ccatgtcctg aatcggttca tcgcaaagtt
     1021 aattgatctg ttcattgtcg tcgctgccgg cgaaatcgct cctccggtcg gttttctttc
     1081 cggcttggcc tacatcttga ttgccgatgg ctttgcgggt ggaaaaagca tcggcaaacg
     1141 gttggtcggt ttgcaaacca tgcgattgga tggccgggat acggccggtt tccgagagtc
     1201 gatcatccgg aatcttccgc tgggtggagc acagatcgcg tatacggtcc catacattgg
     1261 atggctcgta tctctggcca ttttagcatt tgaaagtttt ttgatcattg gaaatgagca
     1321 agggcgtcgg cttggcgatg aagtagcaag gactcacgtg ttagacgccg gtcaacttgc
     1381 ggtcccggat taggtaagtc gtacacgaag cacacaggct ggggccggat cgcccagcca
     1441 atatgaaggg agagacgccg tggggttccc gggaaacatg tttggttggt tctccgatga
     1501 cctggcgatt gacttaggca ccgcaaccac gctcgtgtat gttcacggga aggggatcgt
     1561 tctgaatgag ccctctgtcg ttgcagttga aaagaaaagc gaaaaagtgc tggccgtcgg
     1621 agcggacgct aaaaagatgc tcggacgcac gcccgggaat atcgtcgcgg ttcggccgat
     1681 gaaggaaggc gtgatcgccg acttcgagat ggccgagcaa atgctgaagc atttcatccg
     1741 gaaggcccac aatcgcagtg cgtttgtgcg gccgcgaatc atcatcggcg tgccctcccg
     1801 catcacgcag gtggagcaac gtgccgtacg cgattccgcc gaattggccg gggcacgaga
     1861 agtctatctg atcgaagagc ccgtcgctgc agccatcggg tccggattgc cgattacgga
     1921 accgtccgga aacatggtgg ttgacgtggg aggcggcacg acggacatcg ccgtcatctc
     1981 acttggcggc atcgtgtaca gtgagtccgt caaggtcgcc ggtgatcgca tggatgaagc
     2041 gatcatgaat tacatcaaga aaaagtacaa tctgctcatc ggtgagcata tggcggagcg
     2101 aatcaagttt gaaattgggt ccgcctaccc gttcgaggag cggaaaacta tgatgatcaa
     2161 ggggcgggat ttgatttccg gcattccgcg cacgttggtc atcgatgacg ctgagattcg
     2221 tgaggcgttg caagaaccca tcggaacgat cgtgaatgcg atcaagatcg cattggaaaa
     2281 caccccgccc gagctggctg gggatattat cgaccgcggg atcgtgttga cagggggagg
     2341 ttccctcctg aaagggatgg acacacgatt ccgagaagaa acaaacctgc cgatcatcac
     2401 ggtggacgat ccgctgacct ctgtcgtgtt gggggtcggc aagattctgg atgagctgga
     2461 tctcctccga aaggtatcgg tcatgtctca ggcgaacaac ctccggtaag gttccatccc
     2521 tcatgcggat ggtcaattta cgcgcaccgt acagcgccag gcgtgttgcc ctcattcttg
     2581 ccgtcattct cgtgctcggc ttcctgcttc ttccaggcca gctccaaagt ggcttccaat
     2641 acataggcag tccgttagga tggattatca gctggcctct ccaggccgtg gccggaatcc
     2701 atgatcggat tgccgatatc tggggacgat atgtggccct ccaagaggtt gaagaagaaa
     2761 atcagcagtt gaggagagaa ctggaccttc tcagggaaca aaacgggcag ctgcgagaag
     2821 cttcagtggc gacggagcgg ctctcagcgc tactagagtt caagaagcaa gtccttccca
     2881 catccgtggc cgcccaagtg atcgggcgcg ataccggcaa ttggtataag acgatcattc
     2941 tgaacaaggg tgcgtccgac ggccttcaat cggaccaggg cgtgattact ccggcaggtg
     3001 tggtcggtcg cgtcgtcaag accacgggct ccaccgccgt cgtcctgctc gtgacggatc
     3061 ccaataatgc gattgccgga ttgatccagc gc
//
LOCUS       DASTWE010000788         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_797108, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000788 DASTWE010000000
VERSION     DASTWE010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797108"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFT30_08175"
     CDS             complement(<1..531)
                     /locus_tag="VFT30_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEU5092647.1"
                     /translation="MSGKSKATLLLTLVIGAMALVGTAIPLTDHPTFCAGCHTIAPAY
                     ESWAKSSHKEVTCVACHVRPGVQGWLSDKVLAGVRDATIYVFGTPTDAHNLKAKVDSE
                     VCLSCHRHILRVSETAPRDLPSPVKEVGLVMSHRQHMEAFKARGQDEGCTTCHAGVVH
                     DEPIKGYPIVIPRGHVS"
     gene            complement(515..1033)
                     /gene="mobB"
                     /locus_tag="VFT30_08180"
     CDS             complement(515..1033)
                     /gene="mobB"
                     /locus_tag="VFT30_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEU5092648.1"
                     /translation="MSIPIVSFVGRSNSGKTTLIERVIPELVKAGYRVATVKHAGHGF
                     DLDTEGKDSWRHKRAGAASVVVVSKGSLALFADVSEQLKVEEVRDRFLDASYDLIIAE
                     GWKSEGYPKIIIVRDQLGEIAYSSDGLLAVVSDKPINLPVPALHLDDVAGVAAILIKQ
                     FPLRRREHEREV"
     gene            complement(1059..>1836)
                     /locus_tag="VFT30_08185"
     CDS             complement(1059..>1836)
                     /locus_tag="VFT30_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEU5092649.1"
                     /translation="LAKPFAFVYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQEAGGIPLLLGIARDTPMALKEKISQGLNADMLVLSGGVSMGDCDFTKVVFRELG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPALLKMSGCRSYG
                     RPVVQAVFQERFSKRGDRRHFLRGILTREEGVFKVRTTGDQGSGILTSMVKANCLIDV
                     PVEVERLNPGDEVVVQLLSGEAWPAKTEHGHSGGDRLSCC"
BASE COUNT          433 a          552 c          457 g          394 t
ORIGIN      
        1 cgatacatgc ccgcgtggaa tgacaatggg ataacccttg atgggttcat catgcacgac
       61 gccggcatga caagtcgtac acccttcatc ctgtccacga gccttgaatg cctccatatg
      121 ttggcgatgg ctcatgacca gccccacctc tttcaccggc gacggaagat cgcgcggcgc
      181 cgtctcggac acgcgaagga tatgacgatg acaactgaga cagacttcgg aatccacctt
      241 tgccttgagg ttgtgcgcat cggtcggcgt tccgaatacg tagatagtcg catctctgac
      301 tcctgccagg accttgtcgg ataaccagcc ttgaaccccg ggcctcacat gacatgcaac
      361 acaggtaact tctttatggg aagacttggc ccagctctcg taagcggggg cgatcgtatg
      421 gcatcctgcg cagaatgtcg gatgatctgt aagcggtatc gcagttccaa ccagagccat
      481 agctccgatc acgagggtca gcaacagcgt ggccttagac ttcccgctca tgctcccgtc
      541 gtctaagggg gaactgcttg attaagatgg cggcgacacc ggccacatcg tccaggtgga
      601 gcgcaggcac gggaagattg atcggcttgt cggaaaccac cgccaggagg ccgtcggaag
      661 agtatgcaat ttcaccgagc tgatcacgga cgatgataat cttcggataa ccttcgcttt
      721 tccacccttc cgcgatgatg aggtcataag aggcatccaa gaaacgatca cgtacttcct
      781 ccaccttcag ctgttcggag acatcggcaa acaacgcgag gctccccttc gagaccacca
      841 caacactagc ggcaccggcg cgtttatggc gccaactatc ttttccttcc gtatcgagat
      901 caaatccgtg gccggcatgt ttaaccgttg ccacacggta cccggctttc accaactccg
      961 ggataactcg ttcgatcaag gttgttttac cgctattcga tcgaccgaca aaagaaacaa
     1021 ttggaatgga catgtgtgac cgttacgtgt tttttgatct agcagcagga gagccgatcg
     1081 cctccggaat gcccatgctc ggttttagca ggccatgcct caccgctcaa taattgaacc
     1141 accacctcgt caccgggatt aagccgctca acttctaccg gtacatcgat gagacaattc
     1201 gccttgacca tagacgtcag aataccggag ccctggtctc cggtcgttcg aaccttgaac
     1261 acaccttctt ccctcgtgag aatgccgcgc aggaaatggc gccgatcgcc ccgcttggaa
     1321 aatcgttcct gaaatacggc ctgaaccacc ggacgtccat agctccgaca cccgctcatt
     1381 tttagtaacg caggtcgcac caattgctcg aaggtgacca tcgatgagac cggattgccc
     1441 gggagaccaa aagcaagctt gccctggatc ttcccgaagg ccagtggttg accgggtcgg
     1501 atagcgagtt tccaaaaatt catctcggca ccgagctcac ggaacactac tttggtaaaa
     1561 tcacagtcac ccatcgatac cccaccggac aacaccagca tgtcggcatt caatccctgc
     1621 gagatctttt ctttgagtgc cattggcgta tcacgcgcga tgccgagtaa taacggaata
     1681 ccccccgctt cctgcacagc cgcggcgatc ccatagctgt tcgaattgat gatcttctcc
     1741 tcgctgaatc gctcgtccaa gtcagccaac tcatcaccag tcgaaaggat cgccacccgg
     1801 ggccgctggt agacaaatgc gaacggtttg gccagg
//
LOCUS       DASTWE010000789         1077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_958891, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000789 DASTWE010000000
VERSION     DASTWE010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958891"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFT30_08190"
     CDS             complement(<1..1041)
                     /locus_tag="VFT30_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5092650.1"
                     /translation="MSIVEVGVEDQSGRMQVVFFNQPYLEEILTVGTRVMMSGRVLSG
                     RQGWMVPRMDVAQYEIIGEDTESTLHVGRIVPIYHETKGWTSRQMRVLVKNLLADHGS
                     ELLDHLPVSLRSRQRLIPIHEAFSDIHFPKTGTDLQLLERGKTAAHRRLAFEELLLLQ
                     LALAARHRSVHEEPKELRFNPRTPFLQQLGRLLPFRLTTAQDRVVREIFRDMISPRPM
                     NRLVQGDVGSGKTAVALHALVMACGSGYQAALMAPTEILAEQHYRNLSGTLQALGLHT
                     TLVRGGEKSSVKRTQVERLASGDIQVAIGTHALIQQGVRFKNLGLAVVDEQHKFGVLQ
                     RKTLIDKGYKPDV"
BASE COUNT          235 a          333 c          266 g          243 t
ORIGIN      
        1 cacgtccggc ttgtagccct tgtcgatcaa tgtctttcgc tgcaggactc cgaacttgtg
       61 ctgctcatcc accacggcca accccaagtt cttgaatctc accccctgtt gaatgagggc
      121 atgagttcca atcgccacct gaatgtcacc cgatgccagc cgctcaactt gtgttctttt
      181 caccgaggat ttctctcccc cacgcacgag agtcgtgtga agtcccagag cctgcaacgt
      241 tccggagagg tttcgatagt gctgctctgc tagaatctca gtcggtgcca tcagcgcggc
      301 ctgatagcct gaaccacagg ccatcaccaa tgcatgcaag gcgaccgccg tttttccgga
      361 ccccacatcg ccttgcacca gacgattcat ggggcggggc gaaatcatgt cccgaaatat
      421 ttcgcgaacg actcgatcct gtgccgtcgt gagacgaaac ggcaagagac gaccgagctg
      481 ttgcaagaag ggtgtccgtg ggttgaaccg tagttccttg ggttcttcat gcactgatcg
      541 atgcctggcc gccaaggcca gttggagcag caggagctcc tcgaacgcca atcgtcgatg
      601 cgccgccgtc ttccctcgct ccaggagctg aagatctgtg ccggtcttgg gaaaatggat
      661 atcggaaaag gcttcatgaa tcgggatcaa ccgctgtcgg gatcgaagag atacaggtaa
      721 atggtccagg agctcactcc catggtccgc taacagattc ttcaccaaca cccgcatctg
      781 acgagaggtc caccctttgg tctcgtgata gatcggaacg atgcgaccga catgcagcgt
      841 cgattcggta tcctctccga taatttcata ttgcgctaca tccattcgtg ggaccatcca
      901 tccttgtcga ccggagagca cccgaccgct catcatcacg cgggtcccaa ccgtaagaat
      961 ctcctccaaa tacggttgat tgaagaacac cacctgcatt cgaccagact gatcttcgac
     1021 gccgacttcc actatgctca accgccgatt tcttgtccgc ttcgccacgc atgttcc
//
LOCUS       DASTWE010000790         1363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_248199, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000790 DASTWE010000000
VERSION     DASTWE010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248199"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFT30_08195"
     CDS             complement(<1..875)
                     /locus_tag="VFT30_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5092651.1"
                     /translation="MPGRRLLATYRLQLNTDFGFSEARDITAYLHDLGITHCYSSSIL
                     AAMPGSMHGYDVIDPSRLNPDLGTDDEFTAWCGSLHDRGMGLILDIVPNHMGIAKALN
                     RWWYDVLENGPSSRYAGVFDIDWHPLKRELHGKVLLPILPDHYGVVLENQQISVAYEE
                     DRFVVRCGDCQLPLAPKSWSAVLSYRLESLLSHDPAQSSMDSGLTELQSILTAIRHLP
                     DRADSTPEREEREREKEVIRKRIAVLMAGDQQMAAFVQNNIHLFNGTAGEPRSFDLLD
                     ELLNQQAYRLASWTVA"
     gene            complement(905..>1363)
                     /locus_tag="VFT30_08200"
     CDS             complement(905..>1363)
                     /locus_tag="VFT30_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092652.1"
                     /translation="RDVGTLDAYWQANMDLLGQAPSFDLRNSEWPILSGGYDGPIASV
                     VQTRVDDSMIGEGSLVVDADIRRSVIGRGVQIEPGAVLDECVIMDGVHIGSRSRLYRV
                     IADRFSSIPAESDIGFQESPHLPGCHISPTGLVIIPRNTKAPMNLMQSVS"
BASE COUNT          315 a          390 c          374 g          284 t
ORIGIN      
        1 gcgaccgtcc acgatgccaa ccgatacgcc tgctgattga gcaactcgtc cagcaaatcg
       61 aagctgcgtg gttctccggc ggtaccatta aagagatgaa tattgttttg aacgaatgcg
      121 gccatttgtt ggtcgcccgc catgagcacg gcgatgcgtt tacggatgac ctctttctcc
      181 cgctcacgct cctcccgctc cggagtgctg tctgcacgat cgggaaggtg gcgaatggcc
      241 gtcaaaatgc tttgcagttc cgtgagtccg ctgtccatag atgactgcgc tggatcatgg
      301 gaaagcaggc tctcaagcct atatgacaag acggcactcc acgattttgg agccagcgga
      361 agctgacagt ctccgcagcg gaccacgaac cggtcttctt cataggcgac cgaaatttgt
      421 tgattctcca gcacgacacc gtagtgatcc ggcaagatcg gaagcaacac cttcccatgc
      481 aactcacgct tgaggggatg ccagtcgata tcgaatacgc ctgcgtaacg ggaggacggt
      541 ccgttttcca gcacgtcgta ccaccaccga ttcagcgctt tggcaatccc catgtgattg
      601 gggacgatat ccaggattag ccccatgccg cggtcatgca aactgccgca ccacgcagtg
      661 aattcatcat ctgtcccgag gtccgggttc aaacgcgagg gatcgatgac atcgtagcca
      721 tgcatactac ccggcatagc ggcgagaatg gacgaactgt agcaatgtgt gatcccgagg
      781 tcgtgcagat atgcggtaat gtcacgcgcc tcgctgaatc cgaaatcggt attgagttga
      841 agccgatacg tggccagcaa gcgacggccg ggcattacga ctccatggtc tcaagaaacg
      901 ggtctcaaga aacggactgc atcaaattca tcggcgcctt ggtgttgcgt ggaatgatga
      961 caaggcctgt cggcgagata tggcacccgg gaaggtgagg cgattcttgg aaaccgatat
     1021 cgctctctgc gggaatactg ctgaagcggt ccgcaatgac gcgatagagc cgagaccgcg
     1081 atccgatgtg gactccgtcc atgatgacgc attcgtccaa caccgcccca ggctcaatct
     1141 gaacgccacg cccgatcaca gaacgtctga tatcggcatc gacgacgagg cttccttctc
     1201 cgatcattga gtcgtcgacg cgagtttgca ccaccgaggc aatcgggccg tcatatccgc
     1261 ccgagagaat tggccattca ctgtttcgta ggtcaaacga cggggcctgt cccaataagt
     1321 ccatattggc ctgccagtac gcatctagcg taccgacatc acg
//
LOCUS       DASTWE010000791         1279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1314320, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000791 DASTWE010000000
VERSION     DASTWE010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314320"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFT30_08205"
     CDS             complement(<1..331)
                     /locus_tag="VFT30_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU5092653.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQQGVFILRIED
                     TDQSRSTDESIQAIIAGMKWVGLDWDEGPFRQTARMDLYRSHAMKLFEAGHAYWCVCK
                     AEELEARR"
     gene            469..1173
                     /locus_tag="VFT30_08210"
     CDS             469..1173
                     /locus_tag="VFT30_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5092654.1"
                     /translation="MAELTQPAKVLAVTDLTPHVRQLVVKPKTSKISFHPGQWVSLKL
                     PVGPKQPLNRAYSMADPSSPYGELTLVFDRIPGGLGSNYLYELRSGDEVPLSGPYGNF
                     ILPQPLDRELLLIGRYTGLVPLRCLLKQMFFSKIQTPVLLIAVAPNEDEILFHQEWLT
                     MAMQHPSFRYLPLVARNGESEAVEKTLSMLTPLVKGQPKVVPMLCGTKAFVRPLRSYF
                     TEGGYDRKEVKVETYD"
BASE COUNT          294 a          375 c          300 g          310 t
ORIGIN      
        1 ttcgtcgagc ctccaactct tctgccttac acacacacca gtaggcatgc cctgcctcaa
       61 acaacttcat ggcatggctg cgatagagat ccatccgtgc cgtttggcgg aatggtccct
      121 cgtcccaatc aaggccgacc catttcatgc ccgcgatgat ggcctgaatc gattcatccg
      181 tcgagcgact ttgatcggta tcctcgatgc gaaggataaa cacaccctgt tgttggcgcg
      241 cgaagagcca attgaacaga gccgtacgca ctcctcctat gtggagaaat cctgtcggac
      301 tgggggcaaa ccggacgcga acttggctca tggccgtgcc gtcccttctt cacgcatctt
      361 cacgcacaac agaccggaca tctataccat gctgtgaaaa tccttgtaca tcgccttacc
      421 acttccatct cctgacggct tctgctatga cggtgtcacg aatcgatcat ggcggaactc
      481 acgcaacctg cgaaggtgct tgcagtcacc gatcttacac ctcacgtccg ccaactcgtg
      541 gtcaaaccca agaccagcaa gatttccttt cacccgggac agtgggtgtc cctcaaactg
      601 ccggtgggac caaagcaacc gctcaaccgc gcatattcca tggccgatcc ttcgtcgccg
      661 tatggggaat taacgctggt atttgatcgt atccccggtg gactgggctc caattacctc
      721 tatgagttga ggtctggaga tgaagttccc ttgtcaggcc cttacggaaa tttcatccta
      781 cctcaaccac ttgatcgaga actcctgctg attggacgct acaccggtct tgtcccactg
      841 cgatgccttc tcaagcagat gtttttttcg aaaatccaaa ctcccgtctt attgattgcg
      901 gtggcaccca acgaagacga gatcttgttc catcaggaat ggctcacgat ggccatgcaa
      961 catccctctt tccgctatct tccgctggtc gctcggaatg gggagtcgga agccgtggaa
     1021 aaaaccttgt ccatgctcac gccgctagtc aaaggacagc ccaaagtcgt tcccatgctg
     1081 tgtgggacca aagctttcgt ccgccccctg cgttcatatt ttacggaggg agggtacgat
     1141 cgaaaggaag taaaagtaga gacttacgac taaaagttat tcgtggatcg ttaatcgcgg
     1201 gtccttttct gcgtttaacg aatatctcgc gacgaatcta gacgaatcag tggccctccc
     1261 gcacaagatt tttattgac
//
LOCUS       DASTWE010000792         1229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1088205, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000792 DASTWE010000000
VERSION     DASTWE010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088205"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1229)
                     /gene="asnB"
                     /locus_tag="VFT30_08215"
     CDS             complement(<1..>1229)
                     /gene="asnB"
                     /locus_tag="VFT30_08215"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5092655.1"
                     /translation="VFNGEIYNFKELRTHLVSRGHAFRTDTDTEVIVHLYEEYGDECI
                     GKLRGMFTFAIWDERSKKLLLARDRVGIKPLYYSFNSQRFIFASEIKALIADRSVRRD
                     IDPEGIDRFLTFMYMPGEKTLFRDIVKLDAGHFLTVQNGKVSIRQYWDLQFEQPAKDI
                     NIEAATTELVNLLGETVRDHMISDVPVGVLLSGGVDSTALLSFVVEASEKEVETFTVG
                     FDGRTFADERPYARLAAQTFGSRHHEMTISAKEFLDFLPKYVWHMEEPVCEPPAVALY
                     YVSKLASNHVKVLLSGEGGDEAFAGYQNYRNLMWVERAKRYGWPVTGVLSTLFEHAGR
                     LSGLEKFRKYSCFLRTPIEDYYYGRTWNPLSAFHGLRTQAYSESSEGTSKMVKALFER
                     VNKDDILNRMLYVDSKT"
BASE COUNT          288 a          339 c          302 g          300 t
ORIGIN      
        1 tgttttcgag tcaacgtaga gcatccggtt gagaatgtca tccttgttaa ccctttcgaa
       61 caaagccttg accattttgc tggtaccttc agagctttcg gagtacgcct gcgtcctcag
      121 accatggaat gcgctcaagg gattccacgt gcgaccgtaa taatagtctt cgatgggagt
      181 tctcaagaaa caggaatatt tcctgaattt ttcgagtccg ctcaatcgcc cggcgtgttc
      241 gaaaagcgtt gacagcacgc cagtcacggg ccagccatac ctcttggccc tttcaaccca
      301 catgagattc cgatagttct gataaccggc gaacgcctca tctcctccct cgccggaaag
      361 taagaccttg acatggttac tggcgagttt tgagacgtag tagagcgcaa ctgccggagg
      421 ctcgcaaacg ggctcctcca tatgccagac atatttcggc aagaagtcaa gaaactcctt
      481 ggccgaaatc gtcatttcat gatgtctgga gccgaacgtt tgggcggcga gcctggcgta
      541 cggtctctcg tcggcaaagg tcctcccgtc gaaaccgacc gtgaaggtct caacttcctt
      601 ttcacttgcc tcaaccacga agctgaggag agccgtggag tccacaccgc cgcttaagag
      661 aactcccacc gggacatcgc tgatcatgtg atcgcgaact gtttcgccca aaaggttcac
      721 cagttccgtt gtggcagcct ctatgttgat gtccttcgcc ggctgctcaa attgaagatc
      781 ccagtactgt ctgatgctga ctttcccatt ttgaacagtg agaaagtgtc cggcatcgag
      841 cttgacgatg tcacgaaaca gcgtcttctc accaggcatg tacatgaagg tcagaaatcg
      901 gtctatgcct tccgggtcaa tgtctcgtct gaccgatcga tcggcaatca acgccttgat
      961 ttccgacgca aaaatgaacc gctgggaatt gaaggaatag tagagaggtt tgattccgac
     1021 ccgatctctc gcgagcagca gcttcttaga ccgctcatcc cagatggcaa atgtgaacat
     1081 cccacgcaac ttgccaatgc actcgtcacc gtactcttcg tacaggtgca cgatcacttc
     1141 ggtgtcggta tcagtcctga aagcatggcc ccggctgacc agatgtgttc ggagttcctt
     1201 gaagttgtag atttcgccgt tgaacacga
//
LOCUS       DASTWE010000793         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1314345, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000793 DASTWE010000000
VERSION     DASTWE010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314345"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..428
                     /locus_tag="VFT30_08220"
     CDS             111..428
                     /locus_tag="VFT30_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5092656.1"
                     /translation="MPNPRIEPLKKVLAMDPSDDVAWFGLGKAYMDDGNFEEAATSLR
                     QCVTVKPTYSAAYLALAQSLQKLGRLDECRTVCATGIDISAKNGDAMVTKNLEALKLS
                     LPS"
     gene            527..1033
                     /locus_tag="VFT30_08225"
     CDS             527..1033
                     /locus_tag="VFT30_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5092657.1"
                     /translation="MRFVIRLALNNVTAKTGGPFGAAVFETKTGRLIAVGVNVVEPTN
                     CSLAHAEMVALTNAHRVLKHFDLGADGMPMLELVTSCEPCAMCYGGIIWSGVRNLVCS
                     ARASDATAIGFDEAPKPKNWVATLKRRGISVVRDVYRSEAITVFQRYQQTGGRVYNAR
                     KGSRIATS"
     gene            complement(1119..1970)
                     /locus_tag="VFT30_08230"
     CDS             complement(1119..1970)
                     /locus_tag="VFT30_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092658.1"
                     /translation="MARWRPRRTMKLHSRWHDSPGAVAVAWKRHLQQELPRQLNDHWS
                     LLHRHCADHSSHLRLGRLLHRLAHHDPGVQESALAELELATQLIRVGLRVTFLPESQA
                     RTADLECRLGSERFFVEVTAMVGSAERRRLPVRGIMTGEEPGGDETDRGVILTHRIRA
                     RIRQKAKQLADYCDPVILSISIPRADLLGGRQGRCEQIQVDLKALAGSITVDLTKLRH
                     LSAVLISLWDVEPLPSRAALRLANVEIIERTQHQRTQPRVRMLVQNPSASAPLSESHD
                     ASIRQTL"
     gene            complement(2023..2355)
                     /locus_tag="VFT30_08235"
     CDS             complement(2023..2355)
                     /locus_tag="VFT30_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092659.1"
                     /translation="MNDHELHRVVQYVTASTSYARDTVSDILHTGLGELSALAAHSTR
                     PFEREALLEYVSQWTIKRTGQPEPLVREVLGCAGRWLDEVYDECVARQPEVLGEPSDG
                     DEGAEPVP"
     gene            2513..>3111
                     /locus_tag="VFT30_08240"
     CDS             2513..>3111
                     /locus_tag="VFT30_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU5092660.1"
                     /translation="MIAKRPVRHKTSSLVVSDRWDLTHLVKNPLDDLERYLKALDAQV
                     AQVESARPRLQATMASSDFRSILALTESIAEGSARLGAFAYLWFSEDTKNTKARSFKS
                     QVEERLTAVNNRLLFLDLWWQGVDNENAARLMADASDLRYYLETIRRYTPHTLSESEE
                     KIINVKNVTGRSAVNTLYDVVTNGLTFTMKIGGKARTMNR"
BASE COUNT          728 a          891 c          829 g          663 t
ORIGIN      
        1 gcacgaatat agcgagaact tcgagatacg gttcagatta tcacgcagaa aactccatag
       61 acaatgtgcg tcagcgctgc tatcgtttgc cataaacgac aggagcacac atgccgaacc
      121 caagaattga gccactgaag aaagtccttg ccatggatcc gagcgacgac gtcgcctggt
      181 tcgggctggg gaaagcctat atggacgatg ggaacttcga agaagccgcc acatccttac
      241 gccaatgcgt gaccgttaaa ccaacctatt cagcagccta tctcgcactc gcacagtcac
      301 tccaaaaact gggacgtctg gatgagtgtc gaaccgtctg tgccacaggc atcgacatat
      361 ccgcaaaaaa cggcgacgcc atggtcacga agaacctcga agccttaaag ctttcactgc
      421 catcatgaaa tccagcctct cacccgcttc tccctttcgc cttccatctt ggaccgtcag
      481 tgctatgcgc cgccagcaaa agcccattag atcggctagt gatcgcatgc ggttcgtcat
      541 ccggctcgcg ttgaacaatg tcacagctaa aactggcgga cctttcggcg cggcagtgtt
      601 cgaaaccaag acgggacgac tcatcgccgt cggggtaaac gttgtcgagc ccaccaactg
      661 ttcgctcgcc cacgccgaaa tggtggcgct gacgaatgca caccgagtcc taaagcattt
      721 cgaccttgga gcggacggta tgccaatgct cgaactggtg acgagttgtg aaccctgcgc
      781 catgtgttat ggagggatta tttggtccgg ggttcgcaac ctcgtctgca gtgcgcgagc
      841 gtccgatgca actgcgattg ggtttgacga agctcccaaa ccgaaaaact gggtggcgac
      901 gctgaaacgt cgagggatct ccgtggttcg ggacgtttac cgatcggaag ccatcacggt
      961 ctttcagcgt tatcagcaaa ccggaggtcg agtgtacaat gcccgcaagg gctcgagaat
     1021 agccacatca tgatgcgcta ttgtcgtgtc cattaatttt cgtgatagtc cgttcgtcct
     1081 gatcatgtcg agggactcaa gacttccgga acgctacatt agagagtttg cctgatcgaa
     1141 gcgtcgtgag attcggagag tggagctgaa gcgctggggt tctgaaccag catgcgcacg
     1201 cgaggttggg tgcgctgatg ttgagtccgt tcaattatct caacattggc aagcctcaac
     1261 gccgctcggg agggaagcgg ttcgacgtcc cagagcgaaa tcaacacggc actgagatgc
     1321 cgaagtttcg tcagatcgac cgttatggaa ccagccaaag ccttcaagtc tacttggatc
     1381 tgctcacacc gaccttgacg tcctcccaga agatccgccc gcggaattga aatactcagg
     1441 atgaccggat cgcaatagtc ggcgagctgc ttcgcctttt gtctgatgcg cgcccggatc
     1501 cggtgagtca agatcacgcc acgatcggtt tcatctcctc cgggctcctc gcctgtcatg
     1561 atgccacgca ccggcagccg tcgtcgttcg gccgaaccta ccatcgccgt cacctcgaca
     1621 aagaaccgtt ctgatcccag acggcattct aaatcggccg ttctggcttg cgactcaggt
     1681 agaaacgtga cccgaagccc gacgcgaatc agctgcgtgg ccagttccag ttccgccagc
     1741 gcagattcct gcacaccggg atcatgatgg gccaagcgat gaagaagccg tccgagtcga
     1801 aggtgacttg agtgatcggc acagtgccga tgcaggagac tccaatggtc attgagctgg
     1861 cgtggaagct cttgttgcaa atgacgcttc caagcgaccg caacggcacc aggggagtcg
     1921 tgccagcggg aatgtaactt cattgtgcgt cgtggccgcc accgtgccat caccttctcc
     1981 attccggctc atgaagcgtc ctccacgaca aaccaaagtc gtttatggga ccggctcggc
     2041 gccttcgtcg ccatccgatg gctcgcccaa aacttccggt tgtcgagcga cacattcatc
     2101 gtacacttca tccagccatc gaccggcaca gcccaggact tcacgcacca acggctccgg
     2161 ctggcctgtc cgtttgatcg tccactgtga aacatattcg aggagcgctt cccgttcgaa
     2221 gggtctggtc gaatgtgcgg ccaatgcaga cagctctccg agacctgtgt gaaggatgtc
     2281 tgacaccgtg tcacgcgcat acgatgtgct tgcagtcaca tattgaacaa cccgatggag
     2341 ttcgtgatca ttcatgttgc agtccgttaa aacaatcttc acacctcggc gattaactgt
     2401 caacgcggct gcatttccct ttgattctga tgatttttct ccaatcacgg cacagtcaga
     2461 tgataagatg caattgctcg ccgcttggca gctacgcaaa aggagattgg gcatgattgc
     2521 gaaacggccg gttcggcaca agacttcatc ccttgtggta tctgaccgat gggatctcac
     2581 ccacctcgtc aagaatccct tggacgactt ggagcggtat ttgaaagcac ttgatgcaca
     2641 agtcgcacag gttgagtcgg ctcgtcctcg gctccaggca acaatggcga gctctgactt
     2701 ccgatcgatc ctggcactca ccgaatcgat cgcagagggt tctgccaggc ttggcgcgtt
     2761 cgcctacctc tggttttcgg aagacaccaa aaataccaaa gcacggtcgt ttaagtcgca
     2821 agtggaggag cggctcaccg ccgtaaacaa tcgcctgctc tttcttgacc tctggtggca
     2881 gggtgtagat aacgaaaatg cggcaaggct gatggccgac gccagcgacc tgcggtacta
     2941 cctggagacc atcagacgtt acacacctca cacactttct gaatcggagg agaagatcat
     3001 caatgtcaag aatgtgaccg gccggagcgc cgtcaacaca ctctatgacg tcgtgaccaa
     3061 cggactgaca ttcacgatga agatcggcgg gaaggcacgc accatgaatc g
//
LOCUS       DASTWE010000794         1952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1152947, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000794 DASTWE010000000
VERSION     DASTWE010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152947"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /gene="carB"
                     /locus_tag="VFT30_08245"
     CDS             complement(<1..1633)
                     /gene="carB"
                     /locus_tag="VFT30_08245"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5092661.1"
                     /translation="MPKRTDIQSILMIGSGPIVIGQACEFDYSGTQACKALKAEGYKV
                     ILINSNPATIMTDPEMADRTYVEPITLDVVEKVIDRERPDALLPTMGGQTALNTTMGL
                     VKRGVIEKYRVTLIGASAEAIHKAEDREAFKQAMHRIGLRVPKSGTVHTRQEALTILD
                     TVGFPAIIRPSFTMGGTGGNIAYNREEFERLIDWALAMSPVSQVLIEESLIGWKEYEL
                     EVMRDLKDNVVIVCPIENFDPMGVHTGDRITVAPAMTLTDKEYQRMRDAALRIIREIG
                     VETGGSNIQFGINPANGEMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     ITNDITGVTKASFEPAIDYVVVKIPRFAFQKFKGADPTLTTQMKSVGEVMAIGRTFKE
                     SLQKAIRSLELELNGLASRFGLDRGVPAEFNQPEVIEKLSRVLRTPLPERLWYVADAM
                     RLGFTDEELFATTKVDPWFLEQVRQLVDFERMLVGHAADPAAVLNGELLWDAKELGFS
                     DDRIAQLLGCETTTVQKARTQQGTRGVTYKRVDTCA"
     gene            complement(1652..>1952)
                     /locus_tag="VFT30_08250"
     CDS             complement(1652..>1952)
                     /locus_tag="VFT30_08250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU5092662.1"
                     /translation="ADYIGLKLMAQAGYDPREAVPFWERMSGCPRQMIDKVCFRSQHN
                     IPEFLSTHPSDLSRINQIEAWIPEALKYYHEAQGGEPPEPAPYHPLIGPPDLPAS"
BASE COUNT          411 a          580 c          490 g          471 t
ORIGIN      
        1 cagcacaagt atccacccgt ttataagtga ctcctcgcgt cccttgttgc gtacgcgctt
       61 tctggaccgt cgtcgtctcg catccgagca attgcgcgat ccgatcatca gaaaatccta
      121 gctctttcgc gtcccagagc aactcaccgt tcaggacggc cgctgggtcg gcggcatgac
      181 cgacaagcat tcgttcgaag tccaccagct gtctgacttg ttctaggaac cagggatcta
      241 cttttgtcgt tgcaaacagt tcttcgtccg tgaatcccag acgcatggca tcggccacgt
      301 accatagtcg ttccggtaat ggtgtccgca acacccggct gagcttttcg atcacttccg
      361 gttggttaaa ctcggctgga acacctcgat caagtccgaa gcgggaggcc aacccgttca
      421 gttctaactc cagggagcga atggccttct gcagagattc cttaaaggtg cgtccgatcg
      481 ccatcacttc tccgaccgac ttcatctgcg tggtcaaggt cggatcggcg cccttaaact
      541 tctgaaaggc aaatcttggg atcttgacca ccacatagtc gatcgccggt tcaaatgatg
      601 ctttggtgac accggttatg tcgttggtga tctcatccaa cgtgtatccg atggccagct
      661 tggcggcgat cttcgcgatc ggaaatccgg tcgccttcga cgccaacgcc gagcttcgag
      721 atacccgcgg attcatttcg atgacaacca tctccccatt ggctgggttg atgccgaact
      781 gaatatttga tccacccgtc tcgaccccga tctctcggat gatacgaagg gccgcgtccc
      841 gcatccgttg gtattcttta tcggtcaacg tcatggccgg cgcgaccgtg atacggtctc
      901 ctgtatggac acccatcgga tcaaagttct caatcggaca cacgatgacg acgttgtcct
      961 ttagatcgcg cataacctcc aattcatact ctttccaccc aatgagcgat tcttcaatca
     1021 gcacctgact gaccggactc atggccagtg cccaatcgat caacctttcg aattcctccc
     1081 gattataggc aatgtttccg ccggttcccc ccatggtaaa ggaggggcga atgatcgcgg
     1141 gaaagccgac ggtatcgaga atggtgagtg cttcctgccg cgtatgtacc gtaccgctct
     1201 tcggcacccg caggccgatt cgatgcatcg cctgcttgaa cgcttcgcga tcctccgctt
     1261 tatgaatggc ctccgctgat gcaccgatca gcgtcacgcg gtatttctca atgacccccc
     1321 gcttcaccaa acccatcgta gtattgagcg cggtttgccc gcccatggtc ggcaacaagg
     1381 catcagggcg ctctcgatcg atcaccttct caaccacatc tagggtaatc ggttcgacat
     1441 acgtccgatc agccatctcc gggtccgtca taatcgtcgc cggattgctg ttgatgagga
     1501 tgactttata cccctccgct ttcagcgctt tgcaggcctg tgtgccggaa taatcgaatt
     1561 cacaggcttg gccgataacg atcggtcccg aaccgatcat gaggattgac tgaatgtccg
     1621 tacgtttggg catggtgtct taatagtcgg atcagctggc tgggagatcg ggcggcccga
     1681 tcagcggatg atacggagcc ggctcaggcg gctcaccacc ctgcgcttcg tggtagtact
     1741 tcaaggcctc aggtatccat gcttcaatct ggttgatacg cgacaaatcg gaaggatggg
     1801 tagagagaaa ctccggaata ttatgctgtg atcgaaaaca gaccttgtcg atcatctgcc
     1861 taggacatcc gctcatcctc tcccaaaacg gaaccgcttc ccggggatca tagccggcct
     1921 gcgccatcag tttaagtccg atgtagtcag ct
//
LOCUS       DASTWE010000795         1303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_829749, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000795 DASTWE010000000
VERSION     DASTWE010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829749"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1077)
                     /locus_tag="VFT30_08255"
     CDS             complement(37..1077)
                     /locus_tag="VFT30_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092663.1"
                     /translation="MKAVDELPEQKLVQLIADIDRVSGMTDEVGQTALLALADWRVKL
                     EGIDSAYRRAHWLYVQSRDAFRQAEEIRYADEYQNAQRLWDGFVGPRFLELKDDPLLI
                     EQFKEALQGLLAAGRIHVEIFNRIRSRRGEPDREIRQITVYSEDIPVDEVVFTTSGIR
                     NQARKPVRETAITYEPASGTIEVVGRVKSLREQVASQFAQKLLGVDLSGERLPPRRVD
                     LTPLLDPMSFPVDPQDGIARVKLTRLVISSLDGGLTQWFDVPFGGEDSLHEVLDEQYG
                     EENPLRNQTRPWLARIEVQFEPERNRMKGKKIAVVLSLPTKCNLRGKTEKERLVLDRY
                     LRAWGLLRGADA"
BASE COUNT          268 a          366 c          365 g          304 t
ORIGIN      
        1 gttcaccgcc atgtcgggaa aggggaacag gcttggtcaa gcatccgccc cacgcagcaa
       61 tccccaggcc ctcagatatc gatcaagtac caagcgctcc ttttcggttt tgccccggag
      121 attgcatttt gtgggcaaac tcagaaccac agctatcttc tttcccttca ttcgattgcg
      181 ctcgggttca aattgaactt cgatgcgtgc caaccagggg cgtgtttgat tgcgaagggg
      241 gttttcttcc ccgtactgct cgtcgagcac ctcatgcagg ctatcttcgc ccccgaaggg
      301 cacgtcgaac cattgcgtaa gaccgccatc aagtgaggat atgaccagcc gggtgagctt
      361 cacgcgagcg attccgtcct gcggatcgac cgggaagctc ataggatcga gcaagggcgt
      421 caggtcgacg cggcgcggag gcaggcgttc accgctgaga tcaacgccga gaagcttctg
      481 ggcgaactgg cttgcgacct gctcccgcag cgatttgact cgaccgacta cttcgatcgt
      541 gccgctggct ggctcgtaag taatcgctgt ttcacgcact ggcttgcgtg cctgattcct
      601 gatgccgctg gtggtgaaca cgacctcgtc gaccgggata tcctcactgt atacggtgat
      661 ttgtcttatc tctcggtcag gttcgcccct gcggctgcgg atgcgattga agatctcgac
      721 atggatccgt ccggctgcga gcagaccctg taatgcctcc ttgaactgct cgatgagcaa
      781 cggatcgtcc ttcaactcaa gaaaacgggg acctacgaaa ccatcccaca gccgctgtgc
      841 attctgatac tcatcggcgt agcggatctc ctccgcttgg cgaaatgcgt cgcgggattg
      901 gacgtagagc caatgagccc ggcggtatgc actgtcgatc ccctcaagtt tgactcgcca
      961 atcggcgagg gccaggagcg cggtttgacc gacttcatcg gtcatgccag agacacgatc
     1021 gatgtcggcg atgagttgaa caagtttctg ttccggcagt tcatcaacag ctttcaagaa
     1081 cccttcggaa aattcctgcg ctgacccatc ccagttgaag ttcgctggga tcgggatttc
     1141 actttgtgca aggtgacttt gaaggtctct tggggaaaca cttcgaacga agtgatggag
     1201 gctggacatg gagcgtcaaa cctttcgttt ggttcacgcg ggaacggtcg tactatgcga
     1261 ccttgtggaa tcgcgctata attgtgagcc tttcgaaatg cac
//
LOCUS       DASTWE010000796         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_86381, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000796 DASTWE010000000
VERSION     DASTWE010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86381"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..714
                     /locus_tag="VFT30_08260"
     CDS             43..714
                     /locus_tag="VFT30_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5092664.1"
                     /translation="MTDTNAEMPVLTIAILSSQCLVWLGLQKILESHATVPMVVLPHL
                     WQPSNRPPAENPDVFILDLETERDTLGAINQIRESAPTSKIVLLCGLEDKDRMREAFA
                     AGVDGIILKVQPPAVVLAVIEALYAPASPHVPAGRLGLGIPIMKTVEADPQPLAWPEA
                     LTEREREIICLVRQGLSNKDIAYKLSISDSTVRHHMTSIFDKVGVPNRQKLLVHAHHV
                     RITPV"
     gene            953..>1190
                     /locus_tag="VFT30_08265"
     CDS             953..>1190
                     /locus_tag="VFT30_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092665.1"
                     /translation="MNDQIPIDPAKVQLVRQYLEKFLFSDVQQKSDEQMWTHFSFNER
                     PQRSEIVFHRKFLDDCKNLEEIMEKSIMPLLLANP"
BASE COUNT          290 a          315 c          305 g          280 t
ORIGIN      
        1 ctccactgag aacccagtta tccattcggt taaggagact acatgaccga caccaacgcc
       61 gagatgcccg tcctcacgat tgcgattctt agcagtcagt gtctcgtgtg gttgggctta
      121 cagaaaatcc tcgaaagcca tgcgactgtc ccgatggtcg tgcttcccca cttgtggcaa
      181 ccctcaaaca ggcctccggc tgagaatccc gacgtgttca tcttggatct ggagactgag
      241 cgcgataccc tcggcgctat caaccagatt cgggagtccg ccccgaccag taagatcgtg
      301 ttgttgtgcg gacttgagga taaggaccgc atgcgcgagg catttgctgc cggagtcgat
      361 ggcatcattc tcaaggttca accgcccgca gtcgtgctcg cagtgatcga ggcgctatat
      421 gctcccgcga gtccccatgt ccctgcgggg aggttgggcc tcggaatacc gatcatgaag
      481 acggtcgaag ccgacccaca gccgctggcg tggccagagg cattgactga acgggaacgc
      541 gagattatct gcctggtgag acagggactc tcgaacaagg acattgccta taagttgtca
      601 attagtgaca gcacggttcg gcatcacatg acgagcattt tcgacaaggt cggcgtgccc
      661 aatcgacaga agctcttggt ccacgcgcat catgttcgca ttactcctgt ctaatttgtc
      721 tagaagatgt ggtggatggc tcttgtctct tccgtcgatg ccacacctct cagcagcgac
      781 agctaatcga gaccgttgca gtgttgaata ttcgttaggt tcaatccgcc ccgcatgaat
      841 caggcctagg atcttcacta gactacacaa taatagagtt cggaccatca tagtctgaac
      901 aggtaagagg ttattccaaa ggcagcttat ccactagcga caggagggaa ttatgaatga
      961 ccagatacct atcgatcctg cgaaggttca actcgtacgg cagtatcttg aaaaattcct
     1021 cttctctgat gtccagcaga aatctgatga acagatgtgg acccattttt cattcaacga
     1081 gaggcctcag cgctcggaga ttgtgttcca ccgaaagttt ctggacgatt gtaaaaatct
     1141 tgaagaaatc atggagaaaa gtatcatgcc gttgctcctc gccaatccag
//
LOCUS       DASTWE010000797         1036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_991582, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000797 DASTWE010000000
VERSION     DASTWE010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991582"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..633
                     /locus_tag="VFT30_08270"
     CDS             409..633
                     /locus_tag="VFT30_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092666.1"
                     /translation="MKRLATLSAALILGTPALALAAEHNASYRGIGLIYFTFIAGILI
                     YGVNDAFGKKAMYVAAPIILGWCYWMLPPN"
     gene            complement(719..>1036)
                     /locus_tag="VFT30_08275"
     CDS             complement(719..>1036)
                     /locus_tag="VFT30_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092667.1"
                     /translation="NEYMPLMAVARMDSLIGEDETRGESDAAGVPEPAISLHEQKQDL
                     RDLHRLLVGTVARNSGIDHRRLNAELIARTGSRVDQATTEQLRKRIQLLERWKELGYD
                     GKR"
BASE COUNT          219 a          307 c          252 g          258 t
ORIGIN      
        1 ttcgatgaac aaaagctgaa gcatctggga tgagctaact ttacgccgag cgatgcttcc
       61 cccaacttac ggtatcacgg ccaccgacat gatcttatag acacttcaag ccagcgcgcg
      121 gtaacagcgc catcttgagc caattgtgcc aacgtctctc tctatcgcat tagctcatcg
      181 ctggtttgac ttcccggaca agaaggttca gcatgattag ccccattaag acaaagaggt
      241 gttctgtgac ttcttgctag gtttagttga gctcaccata gaacgtcatc caagaacgac
      301 atcgcgccct cctggtgtcc atcgccacga gggcgttctt gcatccgcaa cagaaacctg
      361 ccgagacaag acatcccgtg tttttacctg tactggaagg agctcgcgat gaaacgttta
      421 gccacactct cagccgcgct gatccttgga acacctgccc tcgctttggc ggcagagcac
      481 aatgccagct atcggggaat tggattgatc tactttacct tcatcgctgg catcctcatt
      541 tatggggtaa atgatgcctt cgggaaaaag gccatgtatg tggcagcccc gattattctc
      601 ggctggtgct attggatgct gccgccgaac taaggccgat tgattcacca tactaaacag
      661 agccgcgtgc tgattgactt cagtggctct cctgtttgcc actacggagc tcagcgcatc
      721 accgttttcc gtcatagccc agctctttcc accgctccag cagctgaatg cgcttccgaa
      781 gctgctcagt cgtcgcctga tcgacgcggc tcccggtgcg cgcgattaat tctgcattga
      841 gccttcgatg atcgattccg ctgtttctgg cgaccgtgcc gacgagcaat cgatgaagat
      901 ctcgtaggtc ttgcttctgc tcatggagtg agatagccgg ttcgggaacg ccagccgcgt
      961 cgctctcccc tcgcgtctca tcttcgccga tcaggctatc catcctcgcc acggccatca
     1021 gcggcatgta ttcatt
//
LOCUS       DASTWE010000798         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_959062, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000798 DASTWE010000000
VERSION     DASTWE010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959062"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFT30_08280"
     CDS             <1..328
                     /locus_tag="VFT30_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5092668.1"
                     /translation="HQKVRLRAERAFSQHAVSQTRERTGVLILVSLLEHQIYVLPDQP
                     LFQRVPMERWSLVVEAAVGRLKAGDIVGGLCQSIQTCGVLLAEVCPGRPGDNPDELSN
                     ELVHEP"
     gene            427..918
                     /locus_tag="VFT30_08285"
     CDS             427..918
                     /locus_tag="VFT30_08285"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5092669.1"
                     /translation="MREQDEAGVGEEERERWVERHSPSKSGPTLPDEVAFIGNDVEFK
                     GIITYSGTIRIDGSLEGEIYTDGGLLVGQKAVLKAKVTAGTIVCHGSIKGDIQAKDQI
                     VLRAPAVVQGSLTTPVLSIEEGVVFNGMLEMKPQAKVEVLREAGPSVGNMPTRPPVQR
                     LAA"
BASE COUNT          256 a          253 c          323 g          229 t
ORIGIN      
        1 gcatcaaaaa gtacgcctca gggctgaacg ggccttctcg caacatgcgg tgtctcaaac
       61 tcgtgaacgg accggtgtgc tgatcctggt gtctctctta gagcaccaga tctatgtgct
      121 tcccgatcaa cccttgtttc aacgagtgcc gatggagcgg tggtcgttgg tcgtcgaggc
      181 tgccgtcggc cgattgaaag cgggtgatat tgttggcggg ttatgccaga gcatccagac
      241 gtgcggcgtt ctccttgccg aagtctgccc cggtcgtcca ggtgacaatc ccgatgaatt
      301 atcgaatgaa ttagtccacg agccatagaa agcctcctcc taagtgatga acactcaata
      361 aaacaccgta gtcaaccgat agataagagg ctcgacggac cattcggata aggagggagt
      421 gtcattatgc gggagcagga tgaggcggga gtaggagaag aggagcggga gcggtgggtg
      481 gagaggcata gcccatcaaa gagcggtccg acccttccgg atgaggttgc cttcatcgga
      541 aacgatgtcg agttcaaggg gatcatcacc tattccggca cgatacggat tgatggatcc
      601 ttggaaggtg aaatttatac ggacggtggc ttgctcgtgg ggcaaaaggc agtgctcaag
      661 gccaaagtta cggctggaac catcgtgtgt cacggaagca tcaagggtga tattcaagcg
      721 aaggaccaga tcgttctccg tgctcctgcc gttgttcaag gtagcctgac aactccggtc
      781 ctctcgatcg aagagggagt tgtgttcaat gggatgttgg agatgaagcc tcaggcgaaa
      841 gttgaggtgc tacgtgaagc gggaccaagc gtgggaaaca tgccaacccg gccaccggtt
      901 caacgactcg cagcatgatg agcgtccaga gcctctggcg cgaatctcta acatgcgcta
      961 ggggcctggc gtctgtagca ctcattccga atgtagatga gtcaaacgga aatcagtcat
     1021 accatggaac gtctttttgt cccggaaccg agactggatt t
//
LOCUS       DASTWE010000799         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_248361, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000799 DASTWE010000000
VERSION     DASTWE010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248361"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..870
                     /locus_tag="VFT30_08290"
     CDS             115..870
                     /locus_tag="VFT30_08290"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU5092670.1"
                     /translation="MIIVIANQKGGVGKTTTAINLAAAIANQGKKVLLVDLDPQSNSS
                     LSFIEPERVNGGAYELFTEPDRPLDELVYETNTENLSIIPSRISLAKLEAKLIGDFDA
                     AFKLRDRIEKLKGKFDIILIDTPPTLGILTVNGMVAAQHIIIPIQSSYFALEGTDDLL
                     ETIEKIRSRPNPDLNLLGVVVTLFDKRTLIAKDVEAHIRNVFGKKAFKTVISRSVRLE
                     ESPAHKKPIFDYAPRSSGAEEYQSLSKEVLKRV"
BASE COUNT          278 a          297 c          271 g          220 t
ORIGIN      
        1 ggcccgatct gatcggttaa agcaattgag tcggctggtc actgccgacc gctcccaagc
       61 ttaggtcaca catttcttta aaatccgccc gcgacgagcg ggactccgaa cggaatgata
      121 atcgtaatcg cgaaccaaaa aggcggggta ggtaaaacta caactgccat caacctcgct
      181 gccgcgatag caaaccaagg caagaaggtc cttcttgtcg atctagaccc gcaaagtaac
      241 agctcgcttt cgtttattga gccggaacgc gtgaacggcg gggcgtatga actcttcacc
      301 gaacccgatc gtcctctcga cgagctcgtc tacgagacga ataccgaaaa cctctcgatc
      361 attccgtcgc gaataagtct tgcaaagctc gaagccaagc tcatcggcga tttcgacgct
      421 gcgttcaagc tccgcgaccg gatcgagaaa ttgaaaggga agttcgacat catcttgatc
      481 gacactccgc caacgctcgg gatccttacc gttaacggca tggtcgccgc tcagcacatc
      541 atcatcccga tccagtcgtc ctactttgca ttggagggaa ctgacgacct tcttgagacg
      601 atcgagaaga tccgttcacg accgaatcct gacctaaacc tgttgggcgt cgtcgtcacg
      661 cttttcgaca agagaacgct gatcgcaaag gacgtcgagg cccatataag aaatgtattt
      721 ggcaagaagg cgttcaagac cgtgatctcc cggagtgtgc gtcttgagga gtctccggcg
      781 cataagaaac cgatcttcga ctacgcacca cgctcatcgg gtgccgaaga ataccaaagc
      841 ctttctaagg aggtgcttaa acgtgtctaa aggcggatta ccaatgggag taaagatgcg
      901 tcacgacgcg cattacgttg aacagatcgc aaccaagtct cgatcgatcg gacgtaccat
      961 accggttacg tcgatctatc cgaatcccga gcagcctcgc agtgagttcg gtgacctgac
     1021 cgagctgacg gaatccatca aggagaaagg cgtactcgag ccgttg
//
LOCUS       DASTWE010000800         1627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1411973, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000800 DASTWE010000000
VERSION     DASTWE010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411973"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFT30_08295"
     CDS             complement(<1..645)
                     /locus_tag="VFT30_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5092671.1"
                     /translation="MQPDPVFFQDLAIVFLAAVAGGVLARIAGQPLILGYVLGGIAIG
                     PFTPGPTISESHTFDLFAEIGVILLMFSIGLEFSVKDLMRVKWVALVGGPLGILLAIG
                     LAVVTGSLIGWSTTQSIVIGAVISIASTMVLARLLGDRGELRSKHGRVMIGIALVEDV
                     AVVAMTVLVPALGEFDSGRLLVIGQALAKALVILVPVGYLGAKVIPPIMTHVART"
     gene            1006..1227
                     /locus_tag="VFT30_08300"
     CDS             1006..1227
                     /locus_tag="VFT30_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEU5092672.1"
                     /translation="MAAKEPAVISLDPGTYYWCSCGRSRDQPFCDGSHEGTGFEPREF
                     TVDAKKEVALCRCKHTKTPPYCDGTHQTL"
     gene            1303..>1627
                     /locus_tag="VFT30_08305"
     CDS             1303..>1627
                     /locus_tag="VFT30_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEU5092673.1"
                     /translation="MNRPDTSPCEDLADRYQALLEVAQAITAQRDLDQLFHDLAHRLP
                     RVVQVNYVDLSLHDPAKKVMRLHTIQANVPADLVGGHEVAIEDCPAGLVWQLQQPLLV
                     PNLSEE"
BASE COUNT          393 a          438 c          466 g          330 t
ORIGIN      
        1 cgttctggcg acgtgggtca tgatcggtgg gatgactttg gcgcctaagt atccaactgg
       61 cacgagaatc accagcgcct ttgccaaggc ttgaccgatc acgagcaatc gtccagagtc
      121 gaactcgccg agggccggta cgagcacggt cattgctacg acggccacgt cttcgactag
      181 ggcgatgccg atcattaccc gtccatgctt tgaacgaagt tcgccccgat cgccgagcag
      241 acgggcgagt accatggtgc tcgctatgga aatgaccgct ccgatcacga tgctttgggt
      301 cgtagaccac ccgatcaggc tccccgtcac gacggccaat ccgatggcca agagaatccc
      361 cagcggtccg ccgacgagag cgacccattt gacccgcatc aggtccttca cggaaaactc
      421 gagtccgatc gaaaacatca ggagaatgac gccgatttcc gcaaaaaggt caaacgtatg
      481 agattccgag atagtaggtc ccggtgtaaa cggaccgatg gcgatcccgc ccaggacgta
      541 gccgagaatc aacggctgcc cggcaatcct ggcgagtacg ccgcctgcga cggccgcaag
      601 gaatacgatg gcaaggtctt gaaagaatac gggatcaggc tgcacgtaac acctttcaga
      661 agaatggttg gtttggagca acgtacatac cataaaaagg gtgaaacaat accgcgggct
      721 catgaattaa tcgttgtgtt gaccaaaagt tctggtatgg ccatccgctc aaaatttgct
      781 tggataccaa gggaaaggaa agcccctcgt tgcatacacc gaggcgggtt tgtaaatggg
      841 gtgacagaac cagggaacag ttctaccttt tagggcagac ccaggaagat gaaatcagtc
      901 gaaggaacta gagaaggtgc gaagtttgcg atgaaacgat atacttccgc cgcatagtcc
      961 gatcaaacca cgacagaggg aggaagactc atgggaccgc cacgcatggc agccaaagag
     1021 ccggccgtaa tttcgctgga cccgggaacc tattattggt gttcgtgtgg acggtcccgg
     1081 gatcaaccgt tttgtgacgg ttcacatgag ggaacagggt tcgagccgcg tgagtttact
     1141 gtggatgcga agaaagaggt tgctttgtgc cggtgcaagc acaccaagac gccaccatac
     1201 tgcgacggca cgcaccagac gctttgatgc gccgcaaact gcggttcacc gcttctgctg
     1261 ccccttgagc cgttgccggt cgatggtcca tactgcttcc ctatgaatag gcctgacaca
     1321 tctccctgtg aggaccttgc ggatcggtac caggcacttc tggaagttgc gcaagcgatt
     1381 actgctcaac gcgacctcga tcaactcttt cacgatcttg cccaccgtct gccacgggtg
     1441 gtgcaggtca attatgtcga tctctccctg catgatcccg ccaagaaggt gatgcgtttg
     1501 catacgattc aggcaaatgt gccggctgac ctcgttgggg gccatgaggt ggcgattgaa
     1561 gattgtcctg ccgggttagt gtggcagttg cagcagccac tgttggtgcc gaacctatcg
     1621 gaagaac
//
LOCUS       DASTWE010000801         1902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_248418, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000801 DASTWE010000000
VERSION     DASTWE010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248418"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /gene="ndhC"
                     /locus_tag="VFT30_08310"
     CDS             <1..339
                     /gene="ndhC"
                     /locus_tag="VFT30_08310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5092674.1"
                     /translation="PVFLFIFVTLAFGVVTLLVSYFVQPRYPEPEKLSTYECGAEPFS
                     DARMPFPVRYYIFAMLFVIFDIEVIFLYPWAVVFTKIGVIGLIEMMIFIGLFIVAYVY
                     AWRKGALEWD"
     gene            347..874
                     /locus_tag="VFT30_08315"
     CDS             347..874
                     /locus_tag="VFT30_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5092675.1"
                     /translation="MGLIQLGRREKDGTPDVITGSLEKAVNWARKGSLWPMTFGLACC
                     AIEMMAAVSSRYDMDRFGAGVFRGSPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFIPVDIYVPGCPPTPEALLDGILKLQERIMQKR
                     VFVTQPEQVKASLKV"
     gene            884..1381
                     /locus_tag="VFT30_08320"
     CDS             884..1381
                     /locus_tag="VFT30_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5092676.1"
                     /translation="MHPLLQRIQQTFSGGVTGLAEWRGDVVVTVSRDKLHEVAQFLRN
                     DPGMDFDYIVHVSSVDWPDDEERFEVVWEFYSIRKRHRIRLKTRVPESDCVVDSLTDL
                     WKGADFMEREVFDMMGIRFRNHPDLRRILMPDDYTEGYPLRKDFPLRGKGWRDTFDFL
                     DEVPR"
     gene            1396..>1902
                     /locus_tag="VFT30_08325"
     CDS             1396..>1902
                     /locus_tag="VFT30_08325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU5092677.1"
                     /translation="MSFEDQRTTVYKINPAHPESETLPTLRTEELLLNMGPQHPSTHG
                     VLKVILELEGERLVKSTPVMGYLHRGVEKLAEDGTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLLNLQVPERAEYLRTIVAEVQRIIGHQFWLSAQALDIGAMTVFFYCFRDREI
                     LLDWFDELC"
BASE COUNT          414 a          445 c          544 g          499 t
ORIGIN      
        1 ccggtctttc tctttatctt tgtcacattg gcctttggag tcgtgacctt gttggtcagt
       61 tattttgtgc agcctaggta tccggaaccg gaaaaattat cgacttacga atgtggggca
      121 gaaccatttt ccgacgcccg tatgccgttc cccgtcagat attacatttt tgcgatgttg
      181 ttcgtcatct tcgacattga ggtgatcttc ctctacccgt gggcggtcgt attcaccaag
      241 atcggggtga ttgggttgat tgaaatgatg atttttatag ggctgttcat cgtggcctac
      301 gtgtatgcct ggcgaaaagg cgcgctggag tgggactgag gattgtatgg gactgattca
      361 actggggcga cgtgagaaag atgggactcc ggacgtcatc acggggtctc ttgaaaaagc
      421 tgtgaactgg gcgaggaagg gttctctctg gccaatgacc ttcgggctcg cctgctgcgc
      481 gatcgagatg atggcagctg tttcctcacg atacgacatg gatcggttcg gggcaggagt
      541 gtttcgtggt tcgccgcggc agtcggattt gatgatcgtc gccggaaccg tatgtcgacg
      601 tatggctccg gtaattcgaa agatttacga tcaaatgccg gaacccaaat acgtgattgc
      661 gatgggttcc tgtgccacct cagggaacat ctatgacagt tatagtgtcg tgcagggagt
      721 cgaccgattt attcccgttg acatctatgt gcccggctgc cctccgactc cggaggcact
      781 cttggacggc atcctcaaac tgcaagagcg cattatgcaa aaacgtgtgt ttgtgactca
      841 accggagcag gtcaaggcca gtctgaaggt ctgacgcgtc gttatgcatc ctctgctcca
      901 gcgaattcag cagacgtttt cgggtggagt caccggtttg gctgagtggc gaggcgatgt
      961 ggtggtgact gtctcgcgag acaaactcca tgaagttgct cagtttttgc gcaacgatcc
     1021 cgggatggat ttcgattata tcgtccatgt gagctctgtg gattggcccg acgacgaaga
     1081 gcggttcgaa gtcgtgtggg agttctactc gattcggaag cgacatcgca ttcgactgaa
     1141 gacgcgggtg cctgaatcgg attgtgtcgt tgattccttg acggatcttt ggaaaggtgc
     1201 cgatttcatg gaacgcgaag tcttcgacat gatgggtatt cgatttcgaa atcatcctga
     1261 tctgcgacgg attttaatgc cggatgacta taccgaaggc taccctttgc gtaaggattt
     1321 tccacttcgg ggcaaaggct ggcgagacac gtttgatttt ctggacgaag ttccacgata
     1381 ggatgctcca ctagtatgtc attcgaagac caaagaacca ccgtctataa gatcaacccg
     1441 gcgcacccgg aaagcgagac gcttccgacc ttgcggaccg aggagctctt gctcaatatg
     1501 gggccgcaac accccagcac ccatggggtg cttaaagtga tcctagaact ggaaggtgaa
     1561 cggttggtga aatctactcc ggtgatggga tacctccaca ggggagtaga aaagctcgct
     1621 gaagacggca catatcatca gttcattcct catacggatc ggctcgacta tgtctgcgcg
     1681 atgtacaaca actttgccta ttgccgtgct gttgaaaaac tcttgaattt acaagtgcca
     1741 gagcgggcgg aatatctccg gacaatcgtt gcggaagttc agcgcatcat tgggcaccaa
     1801 ttttggctga gcgcccaggc acttgacatt ggggccatga ccgtcttttt ttactgtttt
     1861 cgggatcggg aaatcctgct cgattggttc gacgagctgt gt
//
LOCUS       DASTWE010000802         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1056223, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000802 DASTWE010000000
VERSION     DASTWE010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056223"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFT30_08330"
     CDS             <1..1133
                     /locus_tag="VFT30_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5092678.1"
                     /translation="SSGNPASLVTHRGLMNALSQSSRHKILSIAAVGCSCAALVISFF
                     GLAQFDLPITKYVRSVTIHLPWDQLTVPWMAFTSNVGDRIGQGWQLTAVSILLLVGAW
                     AFEKPTIKIVAIQSLIAHGIAALLANGLKHLIGRPRPKFVHSGDWQITLSWASGLDSF
                     PSGHSTASFAVATVLAKRFPLFGPLCIAVALFVALSRVLRGSHFLTDVLGGAVIGILS
                     GFIATAPLRQWRTSIQDGLRHAAVGTSILFALLWVLSRRMEDGMVGILFLTLGAGAVA
                     GGLWIRRTHWVRSGGTAGSRQSDTSVLLIIYGLAAMTTSHLVLSSVGFACMASWLDAI
                     GRNDDHAEESPGWSVIRESALIGGLAIAILILIDARGVLPFQ"
BASE COUNT          250 a          343 c          366 g          319 t
ORIGIN      
        1 tcagttccgg aaatccagcg agcctcgtga cacatcgtgg cttgatgaac gccctgtctc
       61 agtcatccag acacaagatt ttgtcgatcg ccgcagtcgg ttgttcctgc gctgcgctgg
      121 tcatcagctt tttcggtttg gcccagtttg atctgccgat taccaagtac gtgcgatcgg
      181 tgacgattca tctaccgtgg gaccaactca ccgttccgtg gatggcgttc accagcaatg
      241 taggggacag gatcggccaa ggatggcaac tgacagccgt aagcatttta ctgctcgttg
      301 gcgcatgggc ctttgaaaaa ccaactatta agatagttgc catccagtcg ctgatcgctc
      361 acggcatcgc cgccttgctc gccaacggac tgaagcacct catcggaagg cctcgaccga
      421 agttcgttca ctcgggagac tggcagataa ctctttcatg ggcatcagga ctggattctt
      481 ttccatcggg acatagcacg gcgagttttg ctgtcgcgac ggtgctggct aaacggtttc
      541 ccctcttcgg accactctgt atcgcggttg cccttttcgt agcactcagt cgtgtccttc
      601 gaggatcgca ttttctgacc gatgtcctcg gaggggcggt gatcggcatc ctcagcggct
      661 tcatcgcgac ggccccatta agacagtggc gtacttcgat acaggatgga ctacgacatg
      721 cagcggtggg aacatcgata ctgtttgcct tgctctgggt tctgtcccgt cgaatggagg
      781 atgggatggt gggcatccta ttcttgacgc taggagctgg tgcggtagcg ggcggcttat
      841 ggatccgacg aactcattgg gttcgcagcg gaggaaccgc agggagtcgg caatcggata
      901 cctcggtgct gttgatcata tacggcctgg ctgccatgac gacctcacac cttgtcctct
      961 cgtcggtcgg attcgcctgc atggcatcat ggctcgatgc gataggtcgg aacgacgacc
     1021 acgctgagga atcgcctggc tggtccgtga tacgggaaag tgccttaatc ggaggcttgg
     1081 ccattgccat tctgattctc attgatgcgc gaggggttct cccttttcag tgaccctcat
     1141 tacgcagaag atgtaatggt ttcgttcgac attccctcca agctgtcttg ctgacgatat
     1201 ttgcatccag aagaagctct caaaagcgga gtcgagtgta ggttagtgcc caggagtctt
     1261 cggcggcaat gggacgcc
//
LOCUS       DASTWE010000803         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_86507, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000803 DASTWE010000000
VERSION     DASTWE010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86507"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VFT30_08335"
     CDS             <1..1230
                     /locus_tag="VFT30_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5092679.1"
                     /translation="CPTYRGLELGRYLTILDSTNPMHRLWSEGHWNKLTVAHGCYWKQ
                     CTFCDIGLTYISRYEMTPTERLIRQIEQLIAETGRRGFHFVDEAAPPSALKSLALALL
                     ERRIDITWWGNIRFETAYSPDLCRLLSASGCIAVTAGLEAASDRLLDNMKKGITVDQT
                     ALVAAGFKDAGILIHAYLMYGCPSETIQETIDSLERVRQLVAADLLQSAFWHRFTVTA
                     HSPVGLDPTAHGLRILGPEFGGFAENDLLHHDDRGETPTWLGEGLRRSLLNYLERRGL
                     DLDVRQWFDEEVPRPHVPLTWLRRLLKKRTIDDHSDLERRLVWLGGAVAYEPNGKRAT
                     LTVTGSRQDHVISLPEPEAQWLEQLIKDATPRQASQTYPHMREACQRFPGRASTFNAF
                     LSTSSWKKTRDAGLVLV"
     gene            complement(1386..2663)
                     /locus_tag="VFT30_08340"
     CDS             complement(1386..2663)
                     /locus_tag="VFT30_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092680.1"
                     /translation="MKEHVARRWKQFVVAGAMTALVAGMGSTLPSAYAGGTIKADEDK
                     WISIGMGIRTSFNSIEYAAGGHYSNDFNVNNARIYINGQIHKYVKFTFNTECFNCGVG
                     AGANHFGGNSNIGLLDAIGKFEINEMVNLWVGRTLVPSERGELNGPFYHATFDGFRTP
                     FNPADFSGNFPSGPNGGGVPNSGQAGLYGRDNGAVFFGKVHPFGTHLLYVASVFTGTR
                     GGPNNTGSLLYAGRLQWNLLNDEDNPGYYTSGTYYGTAGDIFAISGNVIHQKDGAGSP
                     TLGRSDFTGAIVDLLIEKVLPNNMGVFTFNGEFKRYWANYGTGAFAGGDCFCIFNGHS
                     WTVYGLYLIPHEVGIGRFQPYVRFVSIDPLYSALRQEWEYGMNYIISGHNARISVFGR
                     YGDIETKSFVSNFGPNATGNKVDSFHVALQLQY"
     gene            complement(3155..3790)
                     /locus_tag="VFT30_08345"
     CDS             complement(3155..3790)
                     /locus_tag="VFT30_08345"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU5092681.1"
                     /translation="MAIRLGDDAPNFTAETTEGTINFHEWLGNSWGILFSHPKDYTPV
                     CTTELGAVAKISPEFKKRGVKVIAVSVDPMDSHKGWINDINETQKTTMNYPIIADPDK
                     KVATLYDMIHPNAIDNMTVRSVFIIGPDKKVKLTLTYPASCGRNFDELLRVIDSLQLT
                     SKYKVATPANWKDGEDCIITPAVGDAEAKDLFPKGFKTVKPYLRYTPQPNK"
BASE COUNT          939 a         1096 c         1002 g          960 t
ORIGIN      
        1 tgtccaacct accgcggcct ggaactgggt cgctacctga ccattctcga cagtaccaat
       61 ccgatgcatc gcctctggtc ggaaggccat tggaataaac tcactgtggc acacggctgc
      121 tattggaaac aatgtacgtt ctgcgatata gggctcacct atatcagtcg gtacgagatg
      181 acaccgaccg aacggctcat ccggcaaatc gaacaactca tagccgagac gggccggaga
      241 gggtttcact tcgttgatga ggccgcgcca ccatcagcat tgaagtcgct cgccctggct
      301 ctcttggagc gacggatcga tatcacctgg tgggggaata ttcgctttga gaccgcttat
      361 tcaccggacc tttgccgtct tctttccgcc tccggttgta tcgcggtgac ggctgggctt
      421 gaggcggcct cagatcggct actggacaat atgaagaaag gcattaccgt cgatcagacc
      481 gcactggtcg ccgccggctt caaagatgcc ggcattctga tccatgccta tctcatgtac
      541 ggttgtccat cggagacgat tcaggaaacg atcgattcac ttgagcgagt ccgccagctt
      601 gtcgcggcag atctccttca atcagccttt tggcatcgtt ttacagtcac cgctcatagc
      661 ccggtggggt tggatcccac cgcccatggc ttgcgcattc tcgggccgga atttggaggc
      721 ttcgcggaga atgaccttct gcatcacgat gatcgagggg aaacgccgac ctggctcggc
      781 gaaggactgc gacgatcgct cttgaactat cttgaacggc ggggacttga tctcgatgtc
      841 cgtcagtggt tcgatgaaga agtccctcgc cctcatgtac ctctcacatg gctcagacgc
      901 ttgctcaaga agcggacgat cgacgaccat tccgacctag aacggcgttt agtctggttg
      961 ggtggagcag tagcttatga accgaacgga aagagagcaa cccttacggt taccggctct
     1021 agacaggatc acgtcatctc acttccggaa ccagaggctc aatggttgga gcaattgatt
     1081 aaagacgcca ccccgcgaca agcatctcag acctatccgc atatgcggga ggcctgccaa
     1141 cggtttccag gcagggcgtc gacgttcaat gcttttctgt ccacctcttc gtggaaaaag
     1201 acccgcgacg caggtctcgt gctcgtgtaa ctctccaatc tttcatttgt acacgaacac
     1261 tcaaacactg acttcagtgt atttgtatgg gacggtgcga ttcatagtca aaaacaaaga
     1321 gccggaatcc agtaatggat tccggctctt gtttcagaag agacgttctc gtttacgtga
     1381 tttgcttagt actgcagctg taacgcaacg tggaacgaat cgaccttgtt gccggtcgcg
     1441 ttaggcccaa aattactgac gaagctcttg gtttctatat cgccgtaccg gccgaacaca
     1501 gaaattcgag cattgtgtcc ggagatgatg tagttcatgc catactccca ctcctgccgc
     1561 agggcactgt acaaaggatc gatactgacg aatcgtacat agggttggaa ccgtccgatg
     1621 ccgacctcat gtggaatcag gtacaacccg tacaccgtcc aggagtggcc attgaagata
     1681 cagaaacaat cgcctccggc aaaagctcca gtcccgtagt tggcccagta ccgcttgaat
     1741 tccccgttga acgtaaacac gcccatgttg ttcgggagaa ccttttcaat caacaggtcg
     1801 acgatagctc cggtgaaatc acttcgcccc aaggtgggtg aaccggctcc atctttttga
     1861 tgaatcacgt ttcccgagat cgcaaagata tctccagccg tcccgtaata ggtaccgctc
     1921 gtgtaatacc cggggttgtc ttcatcattc aacaggttcc attgcagacg accggcgtac
     1981 aacaggctgc cggtgttatt cggtccaccg cgcgtacccg tgaacaccga ggcgacgtac
     2041 aacaaatgtg tcccgaaggg atggaccttg ccgaagaaga ccgcaccgtt gtcacggcca
     2101 tagagaccgg cctgtccgct gttaggaaca cctcctccat tggggccact tggaaaattt
     2161 ccgctgaagt ctgctgggtt gaagggagtc cggaatccat cgaaggtcgc atggtagaac
     2221 ggaccgttca actcaccacg ctcgctcggc accagggtgc gtccgaccca gaggttgacc
     2281 atctcgttga tctcgaattt cccgatcgcg tcgagcaagc caatattgga gttgccgcca
     2341 aaatgattgg caccggcacc gacgccgcag ttaaaacatt ccgtattgaa ggtgaatttc
     2401 acatacttgt gaatttgccc gttaatgtag atacgagcgt tgttgacgtt aaagtcgttg
     2461 ctgtaatgcc ctccggcagc gtattcaata ctattgaaac tggtgcggat ccccatcccg
     2521 atggaaatcc acttgtcctc atcggccttg attgtgccac cagcataggc actcggcagc
     2581 gtggacccca ttccagccac cagtgccgtc atggccccag caactacaaa ttgtttccac
     2641 cgcctcgcaa catgttcctt catactcatg ccctcccttg tgagtaatta gaaaccactg
     2701 cccaacctac ctatgtccaa aatcgttcat acttaggaaa tcgaggcgag tatgcctaag
     2761 ttgtcgaaac atagcaatat tttttctaat ttgagttcgc ctggacccag gctttccctt
     2821 aatccagacc agatttcccc ctaatcctgg tcagactttc ccttaatcca gataacgagg
     2881 aatgctgccg gcgctgtgta tcaaacaaat ctcttactag acacggatac aatcgggcat
     2941 ggcgaggtgt atcggctgga ttatcggtca ctctccgagg agcagggcat gttcgttcac
     3001 tgattaattc attattttac tagagtacca ccatctacat attttcaaat tgaatgtgtg
     3061 cccgcatcgg aaagcttgac taagaaaaaa aatgaaacga gcaggaagcg ctggacgcct
     3121 cctgctcgct gcctttcaaa aagatcggca gaacttactt gttaggctgc ggcgtatagc
     3181 gcaaataggg tttcacggtc ttgaacccct tggggaagag atccttcgct tcggcgtcgc
     3241 cgactgcggg agtaatgata caatcctcgc cgtctttcca gttcgccgga gtggcgacct
     3301 tgtacttcga ggtcagttga agagagtcga tcactctcaa caactcatcg aagttccggc
     3361 cgcaggacgc gggataggtc aaggtgagct tgaccttctt gtccggaccg atgatgaaga
     3421 ccgatcgcac cgtcatattg tcgatggcat taggatggat catgtcgtac agggtggcga
     3481 ccttcttatc cggatcggcg atgatggggt aattcatcgt cgtcttctgc gtttcgttga
     3541 tatcgttgat ccagcctttg tgagaatcca tcggatccac actgacggca atcactttta
     3601 cgcctcgctt tttgaattcc ggcgagattt tggccacggc acccagctcc gtcgtacaaa
     3661 ccggggtgta atccttgggg tgtgaaaaga ggatccccca actgttaccc agccattcgt
     3721 ggaaattgat cgtgccttcg gtcgtttctg cagtaaagtt cggcgcgtca tcgcccaatc
     3781 gtattgccat gagatacctc cttgtatcgc ggatcaccat caacaagatt ctattatgtc
     3841 gtacaatact gcccctcaac aggcgagttc aagaggtcgc atgttaaaac gtcggacctg
     3901 tcgccacaat gtcaagcagc agtgctcgga gaaacgacat tccgccgacc tgcccctcac
     3961 tgtcgacgat tgtcgcgtga caaaaaagaa gagcttg
//
LOCUS       DASTWE010000804         1664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_312542, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000804 DASTWE010000000
VERSION     DASTWE010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312542"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFT30_08350"
     CDS             <1..367
                     /locus_tag="VFT30_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5092682.1"
                     /translation="PWNQLTDQQKQEFVGLFRTLLTNSYADKIETYSGEGVQYLNERT
                     EKEYAEVRTKVLSGKTEIPLDYRLINRADDWRVYDVVVDGVSLVNNYRGQFTKILRAS
                     SYSDLVDQLRKKSDKLKAP"
     gene            395..1591
                     /locus_tag="VFT30_08355"
     CDS             395..1591
                     /locus_tag="VFT30_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5092683.1"
                     /translation="MRSLAVSAALLLLWGLCPIALSCARADVRLFPIPAVSSSKNDGN
                     DVGMIVPALITGPDGDLKYLVAPMVVYNSIVGTRGTVNLFRYEPGGKELRGIASWTER
                     IERKFLLSYVDPGFSNGRYSIGFSAMHFKNATVRFFGLGPTTAESNESNYTASETRLN
                     WKFGVYANEVTQIAVSQRLRHMQAIQQGATELPFSGAIFPEAPGVGGATILGQRISFH
                     YDTRNNLVTPTDGMAVTAYAELNYNFHQGADPLYSRYGLEIKKMFASESKRAILVIRG
                     DLQATLGSDVPFYEQSSLGGQNNLRGFGVDRYIDKQLIAFSIEERIHILRTRLAGVMA
                     DFEIAPFIDTGQVFNSFKNVSFKDYRITPGIGFRGIIRPNVVGRIDYGFSKEGGAIFA
                     GLDFPY"
BASE COUNT          379 a          410 c          476 g          399 t
ORIGIN      
        1 cccctggaac cagctgacgg atcagcagaa acaggaattt gtcggcctct ttagaacgtt
       61 gctgaccaat tcatacgcgg acaagatcga aacctattcc ggcgaaggcg ttcagtattt
      121 aaatgaacga acggaaaaag agtacgcgga agtcagaacc aaagtgcttt caggaaagac
      181 ggagatcccg cttgattatc gcttaatcaa tagggcggat gattggcggg tctatgacgt
      241 cgttgtggac ggggtcagct tggtgaacaa ctatcgcggg cagtttacga agattcttcg
      301 tgcctcttcc tattcggatc tcgtcgatca gctccgcaaa aaatccgaca agctcaaggc
      361 gccataacgc cgtttgtgga ccatgagtac gtatatgcgc agccttgctg tcagtgccgc
      421 gctcctgctg ctttgggggc tgtgtcccat tgcgctcagc tgcgctcgcg ccgatgtgcg
      481 gttgttcccg attcccgccg tcagttccag caagaacgac ggcaatgatg tcgggatgat
      541 cgtgccggct ctcatcaccg ggccggatgg agatcttaaa tatttggttg ccccgatggt
      601 ggtctacaat tcgatcgtgg gcactcgagg aaccgtcaat ctgttccgtt atgaacccgg
      661 cggaaaggaa ctccgaggca tcgcgtcctg gacagaacga atcgaacgga aattcctgtt
      721 gagctacgtc gaccccggtt tcagtaatgg tcggtatagc attgggttca gtgccatgca
      781 tttcaagaat gccacggtgc gttttttcgg acttggtccg accactgcag agagtaacga
      841 aagcaactac acggcctctg agacgaggtt gaactggaaa tttggtgtct atgcgaatga
      901 ggtcacgcaa attgcggtca gtcagcgctt gcggcacatg caagcgatcc aacagggcgc
      961 cacggaactt cccttttccg gggctatttt cccagaggcg ccgggtgtgg gaggagcgac
     1021 gattcttggc cagcggatca gctttcatta tgacacgcgg aataacttgg tgacaccgac
     1081 ggatgggatg gcggtcacgg cctatgccga gctcaattac aatttccatc aaggtgccga
     1141 ccccctgtat tcccggtatg gtctcgagat caagaagatg tttgcgagtg aatccaagcg
     1201 ggcgattttg gtgattcgcg gtgatctgca agccacgctc ggctcggacg ttccgtttta
     1261 tgaacagagc tcgttgggcg gacagaacaa tctgcgcggt tttggtgtcg atcggtacat
     1321 cgataagcag ctgattgcct tcagtattga ggaacggatt catatactac gaacgcgcct
     1381 ggccggggtc atggcggatt tcgagattgc gccgttcatc gataccgggc aggtgttcaa
     1441 ctcgtttaag aatgtgagct tcaaggacta tcgcattaca ccgggtattg gatttagagg
     1501 catcatccgg cccaatgtcg tgggtcggat tgattacggg ttcagtaaag aagggggagc
     1561 tatttttgcc gggctcgatt tcccttatta accgaaccat ccggtgacca cgccgatcgg
     1621 acaatcttga ccctttatta cgaggccagg agagaggcat ctct
//
LOCUS       DASTWE010000805         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1282149, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000805 DASTWE010000000
VERSION     DASTWE010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282149"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFT30_08360"
     CDS             <1..448
                     /locus_tag="VFT30_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092684.1"
                     /translation="NADAHFALFCNLGELLRIDGESLTSLFGLRRMMNELDRALEINP
                     AHIDALSAKGTLLVKLPSILGGDAEKGEALLEYVVSRAPKAVNARLSLAKVRCEHGRH
                     QEAVTLATDALALAQKHNRPDFIPEARAVLEQLQVNAAKASHKAQF"
     gene            complement(496..906)
                     /gene="arfB"
                     /locus_tag="VFT30_08365"
     CDS             complement(496..906)
                     /gene="arfB"
                     /locus_tag="VFT30_08365"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEU5092685.1"
                     /translation="MLHISSSIAIPDHEIDIHAIRSQGAGGQNVNKVSTAVHLRFDIH
                     TSSLPQVYKDILLKLHDHRISDDGVVTIKAQQYRTQERNREDALNRLRTLIQRAAIPR
                     KKRKATAPTRSSQDRRIERKKRQGRLKALRRTLE"
BASE COUNT          251 a          284 c          279 g          238 t
ORIGIN      
        1 aaacgccgat gcccactttg cgctgttttg caatctgggg gagctgctcc gcatcgacgg
       61 agaatccctc acctcgctct tcggacttcg ccgcatgatg aacgaactcg accgagccct
      121 cgaaatcaat ccagctcaca tcgatgcgct ctcggcaaaa gggaccttgc tggtgaaact
      181 gccaagcata ttgggaggcg atgccgaaaa aggcgaggcg ctcttagaat acgtcgtgag
      241 ccgagcgccg aaagccgtca atgcccgatt atcccttgcc aaagtgcggt gtgagcatgg
      301 tcgacaccag gaagccgtga cattggcgac tgatgcctta gccctcgccc aaaaacacaa
      361 ccgacccgac tttatccccg aagcaagagc ggtgttggag cagcttcagg tgaacgccgc
      421 caaagcaagt cacaaagctc agttctaacc ttctactgtc ccgtcggtct tgatcggaag
      481 cgtgcgtgat caaggctact caagtgttct ccgcagtgcc ttcagacgac cctgtcgttt
      541 cttccgctca atccgtcgat cctgagaact ccgtgtcggt gcggtcgctt ttcgtttctt
      601 tcgagggatc gccgcgcgtt gaatgagcgt ccgtaagcgg ttcagcgcgt cctcacggtt
      661 acgttcctgt gtacggtatt gctgggcttt aatcgtgact accccgtcat ccgatatgcg
      721 gtgatcgtgc agcttcaaca aaatatcttt atagacctgc ggcaaagatg atgtatggat
      781 atcaaatcgc agatggacgg cggttgaaac cttattgacg ttctgccccc ctgcgccttg
      841 cgaccgaata gcgtgtatgt ctatttcgtg atccggaatg gcaatagacg acgaaatgtg
      901 cagcatgctg aatagctagc agagtccata tgcaagtgca atgtcaaaca ggaacataac
      961 ggcgaagaca atgggtctga caaggaagga cagatatgac gaggaaccta gtaatcggtc
     1021 gtgtgaaggg taccacacga acttgtgctt cc
//
LOCUS       DASTWE010000806         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1282153, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000806 DASTWE010000000
VERSION     DASTWE010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282153"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..435)
                     /locus_tag="VFT30_08370"
     CDS             complement(49..435)
                     /locus_tag="VFT30_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092686.1"
                     /translation="MINSYRLFDAIDPFGRTWRCEFRWQQTAISIRHADAVDVKWHLA
                     GSDQTLDKVISLPLPLLMTASSNFDRPITDPWCMKLAAMHLKMMIETWQDMDKNIITL
                     TAQELGEAAATVKTVEMDRRQEVLQD"
     gene            complement(453..1079)
                     /gene="folE"
                     /locus_tag="VFT30_08375"
     CDS             complement(453..1079)
                     /gene="folE"
                     /locus_tag="VFT30_08375"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5092687.1"
                     /translation="MKTGKEIVKLGKPKLQTSEYSEVIAQGVESTLRELGEDPDRDGL
                     RLTPMRASKALQFLTSGYHTNIDRIVNGALFDVEYDEMVVVKDIEFFSLCEHHMLPFW
                     GKMHVAYLPDKKVIGLSKIPRIVDMFSRRLQVQERLTQEVATALQEVLDCRGVGVIAD
                     ARHFCMMMRGVEKQHSSTATSAMLGAFRSDKATRDEFLSLVRLKDDRG"
BASE COUNT          235 a          348 c          303 g          266 t
ORIGIN      
        1 agattcttca cctgctgctc gcaattgcgg cgtgcgccta cgtgggactt agtcctgaag
       61 aacctcttga cgccgatcca tctcgaccgt cttgaccgtt gccgcggcct cgcccagttc
      121 ctgcgcggtc agggtgatga tgttcttgtc catgtcctgc cacgtttcaa tcatcatctt
      181 caaatgcatc gcggcaagct tcatgcacca cggatcggta atgggccgat caaagttgct
      241 gctggccgtc atcaacaacg gaaggggcag ggagatgact ttgtccagcg tctgatccga
      301 gccggccaaa tgccacttca catcgacggc gtcggcatgc cggattgaga tcgcggtttg
      361 ctgccaacgg aattcgcagc gccaggtgcg tccgaaggga tcgatagcgt caaacaggcg
      421 ataactgttt atcatccgat cctattttgc cactagccgc ggtcgtcttt caggcggacc
      481 agagacagga attcgtcccg ggtcgcctta tcgctccgga aggcccccaa catggcgctg
      541 gtggctgtgg acgagtgctg tttctccacc ccgcgcatca tcatgcagaa atgccgggca
      601 tcggcgatta ctcccacgcc gcggcagtcc agcacttcct gcagagctgt cgccacttcc
      661 tgggtcagac gctcctgtac ctgcagccgt cgtgaaaaca tgtccacgat ccgtggaatc
      721 ttggaaagtc cgatcacctt cttgtcggga agataggcca cgtgcatctt tccccaaaac
      781 ggcagcatgt gatgctcgca gagcgagaag aactcgatgt ccttgacgac caccatttcg
      841 tcatactcga catcaaatag cgccccgttg acaatgcggt cgatgttcgt gtggtatccg
      901 gaggtgagaa actgcagcgc tttggaggcg cgcatcggag tcagccgtag accgtcccga
      961 tccggatcct cgcccagttc gcgcagcgtc gattccacac cctgggcaat cacctcggaa
     1021 tactccgaag tctgcagctt gggcttgccc aatttcacga tttccttacc cgttttcatg
     1081 ctcccacctg aacttcaaaa atattcttgc gtgtttcgaa aatgcggatc ctgctgagct
     1141 ttggaaaatc cg
//
LOCUS       DASTWE010000807         1287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_183623, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000807 DASTWE010000000
VERSION     DASTWE010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183623"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VFT30_08380"
     CDS             complement(<1..982)
                     /locus_tag="VFT30_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092688.1"
                     /translation="MQNPLVAWIDRNILSLGREMRLSYLPPLMVYVAYGISGLTGIVG
                     TFFVKDYLGLSAAFIAALGFWAGIPWALKMPIGHTVDLLWRWKSWLVGLGAGLLTISL
                     GIMALLIGEREAMTAILPAEIWYVLSVLLAPIGYVIQDAVADAMTVEAVPRVDDHGRP
                     FDDPTRKLMHTTMQTLGRVAIVGGGILVALINVYVFSGTAGLPQAELVRLYKNIYLMA
                     MVIPVVSVAGVGLAWWLKRRQIQRFVQEGFSPEQARQMADVRDVKNEPTKPNWGILGG
                     SSAFVLFTLMVGLGGFPYREEIVFVGSMSIVLFLMSRLVRELEPDKRITLV"
     gene            complement(1107..>1287)
                     /locus_tag="VFT30_08385"
     CDS             complement(1107..>1287)
                     /locus_tag="VFT30_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092689.1"
                     /translation="YVYHLGRERFGAPVRLLREEYTKTGLATDEDIDHYVARARDPRV
                     WSVYYATVSVMARRV"
BASE COUNT          327 a          394 c          310 g          256 t
ORIGIN      
        1 cgaccaaggt gatccgttta tcaggttcca gctcccgaac gagtctcgac atcaaaaata
       61 gcacgatgct catcgaaccg acgaacacga tctcctctcg atatggaaat ccacccaatc
      121 cgaccatcaa cgtgaaaaga acgaaggctg aacttccacc gaggatcccc caattcggtt
      181 ttgtcggttc gttcttcaca tcacgtacat ccgccatctg ccgggcctgc tccggcgaga
      241 atccttcctg aacgaatcgc tgtatctgcc gccgtttgag ccaccaggct aatccgaccc
      301 ctgccacgga gacaactggg atcaccatgg ccatcagata gatgttcttg tacaaccgaa
      361 cgagttcggc ttgcggcagc cctgccgtcc cactgaagac atatacgtta atgagcgcca
      421 cgaggatgcc accgccgacg atcgcaacac gtccgagtgt ctgcatggtg gtatgcatca
      481 gcttgcgggt tggatcatcg aatggccgac catgatcgtc gacacgagga accgcctcga
      541 cggtcatggc gtctgcgaca gcgtcttgaa tgacataccc gatgggggca agtagaacac
      601 tgagtacata ccaaatttcc gccggcaaga tggccgtcat cgcttcgcgt tcgccgatca
      661 acagggccat gataccgaga ctgatcgtca acagccctgc gcccaacccg acgagccagc
      721 tcttccaccg ccacaagaga tcgaccgtgt gaccgatcgg catcttcaat gcccagggaa
      781 tcccagccca aaacccaagc gcggcgataa atgcggcgga aagtccgagg tagtctttga
      841 caaagaaagt tccaacgata ccggtgaggc ctgaaatacc gtaggccaca tagaccatga
      901 gcggcggcag ataggacaac cgcatctctc gaccgagaga gagaatgttg cgatcaatcc
      961 aggcaaccag tggattctgc atcgtgcgat tagatggcta cgccgctgag ggcctggatg
     1021 ggatccatag aagcagcgat atcaagaatc ttttgatcca acgatccatt taaaagaacg
     1081 ctctctcact cacccccgcc cttttcctaa accctacggg ccatcaccga caccgtggca
     1141 taatagaccg accacacccg tgggtcacgt gcccgtgcga catagtgatc gatatcctcg
     1201 tcggtggcaa gacctgtctt tgtatattcc tctcttaaca atcttactgg cgccccaaag
     1261 cgctctctcc ccagatgata aacgtat
//
LOCUS       DASTWE010000808         1163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1540935, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000808 DASTWE010000000
VERSION     DASTWE010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540935"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /gene="larB"
                     /locus_tag="VFT30_08390"
     CDS             <1..747
                     /gene="larB"
                     /locus_tag="VFT30_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEU5092690.1"
                     /translation="KGLERLLQQVRQGRVTTEQALQRLRSLPFEDLGFASLDHHRSLR
                     QGFPEVILCEGKTTAQIIAIARALIKKQGPFLATRADLSVARAIRRLYRRAQYYPDAR
                     IVAIHTSRQKQLGHILVVTAGTADVPVAEEARVTAEVMGSHVERLYDVGVAGIHRLLG
                     KKDRLFDARVVIVAAGMDGVLPSVVGGLVHCPVIAVPTSRGYGASFGGVAALLTMLNS
                     CAAGVGVMNIDNGFGAACLAHRINMLAEKG"
     gene            complement(770..>1163)
                     /locus_tag="VFT30_08395"
     CDS             complement(770..>1163)
                     /locus_tag="VFT30_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092691.1"
                     /translation="ELKIVGLNIAPTPYTLGNGPLQFSVTIQLPKEVDGSSILEVTSL
                     ISSPSKTSLRFLTHRQPVLIPSGVNGEPERSKVSVELAWDGQDHRKQLAGVGTYSYEV
                     RTKLLVNGEKGLRTVMVAWPKRGLLEVK"
BASE COUNT          251 a          307 c          345 g          260 t
ORIGIN      
        1 aaaggactcg aacggttgtt acagcaagtc cgtcaaggac gagtgacaac cgaacaagca
       61 cttcagcgct tgcgatcgct gccttttgag gatctgggat ttgcttcact cgatcatcat
      121 cggtcgttac gacagggctt ccccgaggtg atcttgtgtg aggggaaaac cacggcgcag
      181 atcatcgcca tcgctcgggc actcatcaag aaacagggcc cgtttctcgc aactcgtgcc
      241 gacctatcgg ttgcgcgtgc cattcgccgt ctgtatcggc gagcacagta ctatcccgat
      301 gcacgcattg tggcgattca cacctccagg cagaagcaac ttggacatat actcgtagtc
      361 accgcgggaa ctgcagacgt gcccgtggcg gaagaagccc gtgtaactgc agaagtgatg
      421 ggaagccacg ttgagcggtt gtacgatgtc ggtgtcgcgg gtattcaccg gttgctcgga
      481 aagaaagatc ggctgtttga tgcgcgggtt gtaatcgttg ctgcgggtat ggacggggtc
      541 ctgccgagcg tggtgggtgg cttggtccac tgcccggtca ttgccgtgcc gaccagccga
      601 ggctatggcg cgagcttcgg aggagtcgct gcgctcctaa cgatgctcaa ctcctgcgca
      661 gcgggtgtgg gagtgatgaa cattgacaac ggattcggcg cggcttgcct cgcgcatcga
      721 atcaatatgt tggcggagaa gggctaggcg taggagaaat aatgggacac tatttcactt
      781 ccagtaggcc tcgcttcggc caagcgacca tcacggtgcg taatcccttt tctccgttta
      841 ccaataattt tgtccgcacc tcatacgaat aggtgcctac cccggcgagt tgtttgcggt
      901 gatcctgacc atcccaagca agttcgacgg aaaccttcga tcgttccggc tccccattca
      961 cccctgatgg gatgagaaca ggctgtcggt gcgtgagaaa tcggagcgag gtcttcgagg
     1021 gagaactaat gagcgatgtc acctccaaaa tcgaggatcc atccacttct ttcggaagct
     1081 gtatcgtgac ggaaaactga agaggcccgt tacccagtgt atacggtgtc ggcgcaatgt
     1141 tgaggccgac aattttgagc tca
//
LOCUS       DASTWE010000809         1484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_894879, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000809 DASTWE010000000
VERSION     DASTWE010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894879"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFT30_08400"
     CDS             complement(<1..1146)
                     /locus_tag="VFT30_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEU5092692.1"
                     /translation="MNYYQESVYLQGHIIDSLVLAKVLDLILMMGGTFDLENVHIGKT
                     REETSHARIRIRTASRALLDDILKAIQPHGASIEREADCRTDRAPADGVLPDDFYATT
                     HLPTQIRLNGRWLDVDQIEMDLAIAVNEAGSVAQAVPMGEVRRGDRIVVSREGVRVIP
                     LQRPQERDVFGFMESQVSAERPHGHTIADIATRMRQLRERYRQKQADSHVLLAGGPAI
                     IHAGGREALTWLIEQGFVHTLFCGNALAAHDMEADLYGTSLGYGLSAGRAVPHGHEHH
                     LRTINRIRAIGSIETAVTSGVIKRGIMAACIRQGVPVVMSGTIRDDGPLPGVITDSVL
                     AQRAMRALIPGVGLALLVASTLHSVATGHLLPATVPTVCVDVNPSVPT"
     gene            complement(1211..>1484)
                     /gene="truD"
                     /locus_tag="VFT30_08405"
     CDS             complement(1211..>1484)
                     /gene="truD"
                     /locus_tag="VFT30_08405"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEU5092693.1"
                     /translation="LFGSRVSWASGEPGRIEEAVIAEAGVTKEVLVAAAKTCGFRGER
                     RALRIPLAELEWSLNDFILVLSFSLPPGAYATSVLRELMKVSPANS"
BASE COUNT          323 a          429 c          423 g          309 t
ORIGIN      
        1 agttgggacc gacgggttca cgtctacaca gactgtgggg acagttgccg gtagaagatg
       61 gcccgttgcc acggagtgta gggtcgaggc cacgaggagc gccaacccaa cgccgggaat
      121 caaggcacgc atcgcgcgct gtgccagaac ggaatccgtg atcactccgg gcaagggacc
      181 atcatcacga atcgttccgg acatgaccac cggtacacct tgcctgatac aagcagccat
      241 gattccccgt ttgattaccc ctgaagtaac ggccgtttca atgctgccga tcgcacgaat
      301 ccggttaatg gttctcaaat ggtgttcatg cccatgcgga actgcgcgtc cggcactgag
      361 cccatagccc agcgacgttc cgtacaggtc cgcctccatg tcgtgggcag cgagggcatt
      421 gccgcagaac aaagtgtgca caaatccctg ttcgatcaac caggtcagtg cctctcgtcc
      481 accggcatga atgatggccg gtccaccggc cagcaagacg tgggaatcgg cctgtttctg
      541 cctgtaccgc tctctcaatt gacgcatccg agttgcgatg tcggctatgg tatgaccatg
      601 aggccgttca gcggacactt gagactccat gaatccaaat acatctcgct cttgcggacg
      661 ctgcagtggt atgacacgca ccccttctcg actgaccacg atccgatccc cacgacgaac
      721 ttcgcccatc ggcacggcct gggcgacaga gcccgcctca ttgaccgcga tggcgagatc
      781 catctcgatt tgatccacgt ccagccatcg accattgagt cggatctggg tgggcagatg
      841 ggtcgtagcg tagaagtcat caggtagcac gccgtcagcg ggtgccctat cggtccggca
      901 atccgcctca cgttcgatcg aagcgccgtg tggttgaata gccttcagaa tgtcgtctaa
      961 gagtgctctc gaggcagttc ggatccgaat acgcgcatga gacgtttctt cccgtgtctt
     1021 gccgatgtgc acgttctcca ggtcgaaggt tccccccatc atcaggatga gatcgagcac
     1081 cttggcgagc acgagagagt cgatgatgtg cccttgaagg tacacgcttt cttggtagta
     1141 gttcatcgcg gcactcacgg aatgtgtata ttttatcacc tattgtcgcg ggagaaagaa
     1201 atgagaggaa ctaagagttc gccggggata ctttcatcag ttcccggagc acgctcgtgg
     1261 cgtaggcgcc gggaggcagg ctaaaggaca ggacaagaat gaagtcgttt agtgaccatt
     1321 cgagttcagc aagggggatg cgaagcgctc tgcgctcgcc acggaatccg caagttttag
     1381 cggcggcgac gaggacttcc ttagtcacac ctgcttcagc gatgacggct tcctcgattc
     1441 tacctggctc gccactggcc catgataccc gcgaaccaaa gagg
//
LOCUS       DASTWE010000810         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1347120, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000810 DASTWE010000000
VERSION     DASTWE010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347120"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..514)
                     /gene="secG"
                     /locus_tag="VFT30_08410"
     CDS             complement(158..514)
                     /gene="secG"
                     /locus_tag="VFT30_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5092694.1"
                     /translation="MLYTLIVIVHVFICFLMIGAILLQSGKGAEIGAAFGGSSQTVFG
                     SRGPANFLSKLTVVVAAVFMVTSLSLAILAKQRNVSSTIIDMQPKSEPTAPSAAPPAQ
                     PSGDSHPSGETPAAGH"
     gene            complement(521..>1067)
                     /gene="tpiA"
                     /locus_tag="VFT30_08415"
     CDS             complement(521..>1067)
                     /gene="tpiA"
                     /locus_tag="VFT30_08415"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5092695.1"
                     /translation="VSAPMLKDLGCRYVILGHSERRTLFGEQNDSIQKKIRAALKHEL
                     RPILCIGESLAQRDNGSTDEVLTQQLRESLSGLTGEAMAGVTVAYEPIWAIGTGKSAT
                     TEQAVAAHRTIRRVLAATASPAITDRTRILYGGSVTPQNIESLLASDQVDGALIGGAC
                     LQVESFATIAKVASAARSIGV"
BASE COUNT          238 a          290 c          314 g          225 t
ORIGIN      
        1 agacgctttc ttttcgatcg tgcgcggaga agatcgcgca cacgagtcct ggctcacacg
       61 tatctaacac gtcaaccgag tgacatctcg agcaacatcg aaccgggatt ggcctctcgg
      121 ttgacgaatg atatgtggtg acctgacgaa gaacaggtca gtggccggcc gccggagtct
      181 ctcccgaagg atgcgaatcc cctgaaggct gagcgggtgg ggcagccgaa ggagctgttg
      241 gttcgctctt gggctgcata tcgataatgg tcgaagagac gtttcgttgc ttcgctaaaa
      301 ttgccagact gagcgatgtc accatgaata ccgccgccac gacgactgtc agcttgctca
      361 aaaaattcgc ggggccacgg ctgccgaaca cggtttgact ggatccgccg aaagcggcgc
      421 cgatctccgc tcccttcccg gattggagaa gaatcgcccc gatcatgaga aagcagataa
      481 agacgtggac aataacaatc aacgtataca gcatgagatc ttagactccg attgatcgag
      541 cagcggaagc aacctttgca attgtagcaa aggactcgac ttggagacaa gcgccgccga
      601 tgagtgcacc gtcgacctgg tccgaggcaa gtagcgattc aatattctgg ggcgtgacgc
      661 ttcctccata gagaatcctc gtgcggtcgg tgatcgcggg agaagcagtg gcggcaagga
      721 ctcttcgaat agtccggtgg gcggccacgg cttgctcggt cgttgccgat ttgcctgtgc
      781 cgatggccca tatcggttca taggcgacag taacaccagc cattgcctcg ccggtcaagc
      841 cggacaagct ctcgcgtagt tgttgcgtga gcacctcgtc cgtcgacccg ttgtctcgct
      901 gcgcgagcga ctcaccgatg cagagaatcg gacggagttc gtgtttgagc gccgctcgga
      961 ttttcttctg gatactgtcg ttctgttcgc caaaaagggt tcgccgttcc gaatggccca
     1021 ggatcacata gcgacaaccg aggtctttaa gcatcggtgc ggagact
//
LOCUS       DASTWE010000811         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_991839, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000811 DASTWE010000000
VERSION     DASTWE010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991839"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFT30_08420"
     CDS             <1..447
                     /locus_tag="VFT30_08420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU5092696.1"
                     /translation="FAPKIQQLQQSRLTVAAESEKKAGTAFMAKAASEKGATKTESGI
                     IITTIKPGTGATPKATDTVKVHYHGTLTDGTVFDSSIKRGEPATFPLNKVIKCWTEGV
                     QQIKVGGKSRLVCPSNLAYGDGGSPPTIKPGATLVFEVELLDIVTN"
     gene            559..1860
                     /locus_tag="VFT30_08425"
     CDS             559..1860
                     /locus_tag="VFT30_08425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5092697.1"
                     /translation="MSETISTNRGDATPPSPLRWLILGLLFSISIVTYIDRVNISVTA
                     RQMMPALGLTEQEMGFVFSAFVIGYALFQIPGGWLGDRWGVRVVLMIALIWWSCFTAW
                     TAIAATSFLAAPLGIVGALALVRFLLGVGEAAALPTFNRAVTDWLPPHERGRGIGIAI
                     GGIGIGAAITPPVTAWIMVNYGWQSAFYLSSGIGFVLAVIWWFLAADHPSHHPWHAHD
                     EAVPSTTPAAPIRPSIPWASLRKTPSVWWLTLSYACLGYVAYVYMSWFYLYLVNERGF
                     SVLRGGLFAAAPFLAILISCPLGGWVTDRLAMRRGVTDGRRIVGMTGMGLAAGSIALG
                     ASAESPYLALASLSLGAGWLYFTVGAYWSSTSDLSPTHAGSLSGLMNMGANLGGAISP
                     ALTPWLAQRWGWGVSLGLAAFISLLGGILWLRIKPGEGLRC"
     gene            1983..2504
                     /locus_tag="VFT30_08430"
     CDS             1983..2504
                     /locus_tag="VFT30_08430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5092698.1"
                     /translation="MALEFRKKDPRATIATKFGEIKIRFYSDAAPRHVENFINLVKMR
                     FYDGTTFHRVVPGFIIQGGDPLSKTPDRALHGTGGPGYFLSPEPSDRPHKRGAVSMAK
                     MPRESNSTRDFNDNGSQFFICVGENSGLDRRYTVFGEVFRGIEIVDKIVGVPRDERDN
                     PLDPITITMTVKE"
BASE COUNT          517 a          702 c          691 g          595 t
ORIGIN      
        1 tttgctccga agattcaaca actgcaacag tctcgattga ctgttgctgc tgagagtgag
       61 aagaaggccg gcactgcatt tatggcaaaa gcggcttcgg aaaaaggagc gacgaaaacg
      121 gagtcaggga tcatcattac caccatcaag cctggtaccg gagccacgcc aaaagcgacg
      181 gatacagtca aggttcatta tcatggcact ttgaccgatg gaacggtctt cgatagttcc
      241 atcaagcgag gggaaccggc gacgtttccg ttgaacaagg tcattaagtg ttggacagag
      301 ggagttcaac agatcaaggt cggaggaaag agccggctgg tgtgcccatc caatctggcc
      361 tatggcgacg gcggatcacc gccgaccatc aagccgggtg ccaccttggt cttcgaagtt
      421 gagttactcg acatcgtcac caactgatca cttcgctttg ccgcctctgc aaagaggcgg
      481 caaagctatt cagtccctcg acgcctgccg actcccagag tggccaaccc ttctgatgaa
      541 tctgatagtg aaaccattgt gagcgagacg atctccacaa atcgcggaga cgccacacct
      601 ccttcgcctc tacgttggct gattctcggg cttctctttt ccataagcat cgtcacctac
      661 atcgatcgcg tcaatatttc ggtcaccgca cgtcagatga tgccggcgtt gggacttacc
      721 gagcaagaga tgggatttgt tttctccgca ttcgtcatcg ggtacgcctt gtttcagatt
      781 cctggcgggt ggctcggtga tcgctggggc gttcgtgtcg ttcttatgat tgcactaata
      841 tggtggtcct gctttaccgc ctggacggct attgccgcta cttctttcct ggccgcgccg
      901 ctcggaatcg tcggcgcgct ggcccttgtc agatttcttc tcggtgtcgg agaggcggcg
      961 gcattaccga cctttaaccg cgccgttacc gattggcttc ctcctcatga gcgcggtcgt
     1021 ggcatcggca tcgctatcgg cgggattggg atcggcgcag caatcacccc gccggtaaca
     1081 gcttggatca tggtcaacta tggctggcaa tccgcctttt atctttcaag tgggatagga
     1141 ttcgtcctcg cggtcatttg gtggttctta gccgcagatc atccatcgca tcacccatgg
     1201 cacgctcatg acgaagcagt cccgtccaca actccagccg ctccaatacg tccttcgatc
     1261 ccgtgggcta gcttacgaaa gacaccgagt gtgtggtggc tgacgctgag ttacgcatgc
     1321 ttaggctatg tcgcctatgt ctacatgtcc tggttctatc tttaccttgt caatgagcga
     1381 ggcttcagtg ttttacgagg tggattgttc gctgccgccc cctttctcgc gatccttata
     1441 tcgtgccctc ttggcggatg ggtcactgat cgattggcga tgcggcgtgg cgtaacggat
     1501 ggacgccgga tcgtggggat gaccgggatg gggttggccg ccggctccat cgcacttggg
     1561 gccagtgcgg aatctcccta tctcgccctt gccagtctgt cactgggcgc cggatggctg
     1621 tacttcacgg tcggtgccta ttggtcctcg acgagcgatc tatcaccgac ccatgccggc
     1681 agcctctcag gcctcatgaa tatgggggcc aatctcggcg gagcaatctc tccggcgctc
     1741 accccatggc tcgcccaacg gtggggctgg ggcgtttctc tcggcctcgc tgccttcatt
     1801 tcgctgctcg gtggaatcct gtggttgcgc atcaagcccg gagaggggct gagatgttaa
     1861 gagtgttacg gcctgaatct ccagttctgg tcttcgatcc aaaacgaaga actcaaaacc
     1921 caacattcaa cagttgctat agccttgcct ctcgaaagat cgccttgcta cattgaatga
     1981 cgatggcact cgagtttcgg aagaaagatc cccgcgcgac gatcgccacg aaattcgggg
     2041 aaatcaagat tcgcttctac tccgatgcgg caccacgcca cgtggagaac ttcatcaatc
     2101 tcgtcaagat gaggttttat gacggtacca cgttccatcg tgtggtgccg gggttcatca
     2161 ttcagggcgg cgatccattg agcaaaactc ccgatcgcgc actccatggc acgggcggac
     2221 caggatactt tctttctccc gaaccgagcg accgtccaca caagcgtggc gcggtatcca
     2281 tggcgaaaat gccccgtgaa agcaatagca cgcgcgactt caacgacaat ggctcacaat
     2341 tttttatctg cgtaggagag aacagcggtt tagatcggcg ttacacagtg tttggagaag
     2401 tctttcgtgg gatcgaaatt gtcgacaaga ttgtcggtgt ccctcgcgac gagcgagaca
     2461 acccactcga tccgatcacg atcacgatga cggtgaagga gtagg
//
LOCUS       DASTWE010000812         1838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_442229, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000812 DASTWE010000000
VERSION     DASTWE010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442229"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFT30_08435"
     CDS             <1..682
                     /locus_tag="VFT30_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5092699.1"
                     /translation="RNPQRDVPIGIIGSLVICTVLYISVAAVLTGLVPVTQIDIHAPV
                     AEALSLVGFKWGAAIVAIGAVAGITSVLVVMMLGQIRVFFAMSRDHLLSPGLSKVHPI
                     FGTPHRATILTGVAVAILAAFFQIGDAADMTNIGTFFAFVLVCAGVMLLRYTKPDRPR
                     PFRIPFMPLVPILSIMACLYLMAGLPWATWIRFGVWTIIGILVYAAYGMKHSKLATQA
                     VNSGSLSR"
     gene            complement(1343..>1838)
                     /locus_tag="VFT30_08440"
     CDS             complement(1343..>1838)
                     /locus_tag="VFT30_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5092700.1"
                     /translation="FRRAIANGVATMMTAHVLYQALDENRPATLSPTIIGRFLREELH
                     YDGVVLTDDLEMHAIIDHYGIGDATIQAIQAGCDMPLICKDRNRVVAAMNAVDKAVAN
                     GDISAGRLAQSLARIRRLKERFLLPYRPVMISDAKLVVGCRSHKALLRSIHQTRERFA
                     RADL"
BASE COUNT          400 a          469 c          506 g          463 t
ORIGIN      
        1 ccgtaaccct caacgagatg ttccgatcgg tattattggc tcactggtta tttgtacagt
       61 gctctatatt tccgtggcag ctgtgctcac cggtctggtc cccgtaaccc aaatcgacat
      121 tcatgccccg gtggccgaag cgcttagcct cgtggggttc aaatggggcg ccgctattgt
      181 tgctatcgga gctgtggcgg gtattacgag cgtattggtt gtgatgatgc tgggacaaat
      241 ccgcgtcttc ttcgccatgt cgcgcgatca cctcttaagc cccggactat ccaaggttca
      301 tccaatattt gggaccccgc atcgcgcaac cattctaacc ggagtcgccg ttgcaattct
      361 tgcagcgttc tttcagatag gggatgcagc tgatatgacc aacattggga ccttctttgc
      421 ctttgtgttg gtgtgtgcgg gtgtgatgct acttcgatac acgaagcctg atcgtcctcg
      481 tccttttcgc atccccttca tgcctctcgt gcctattttg agcatcatgg cctgtctcta
      541 cctgatggct ggtttgcctt gggcaacatg gatccgtttt ggggtatgga ctatcattgg
      601 cattctcgtc tatgccgctt atgggatgaa acacagcaaa ctggccacac aggccgtcaa
      661 cagtggttcg ctgtcacgat gaatcaggga caattcttga tggggctagt ccgactttcc
      721 agaatgtggg gttgtggaag caggtttagc ggtagatgac tcttcagcgg gaactgtggc
      781 agttcgaaca gagttcgtga ggggagcagc cggggcaggc gatccagggg cggtaccaac
      841 tggagaggct gcgggtttag ggggagtaac cggtttggga gacgctggcc tttctggctt
      901 aatgcctcct tcccaggcag gaccagaata catatccgac gttagaccct ttgtcttcca
      961 tttttcctcg aaatcagcag cggccttatt cggtgtttca ccgacaccca tgacatcgtt
     1021 ccaaggatag actttcggtc cgatctgaca acccgattcc gtccttcgta aatacaaagc
     1081 gatcggatta ccccggtagg gcccaagaaa aatatggacg gatccgacgt actcgagcaa
     1141 tgaggccata aggccctcca aggaacgcac atgatgccac aagagatgag aagagggcaa
     1201 gatattcggt ggataagaac ggaggtttcg cttccttact gcattgagat gaggccttgc
     1261 attttgatgg aactcaatga attctccttg gaggcaaatg ctcaaagttg cccaccttgt
     1321 tcttgaaaag aacgaagcag ggttataggt cggctctcgc gaatcgttcc cttgtctggt
     1381 ggatggagcg taagagagcc ttatggcttc ggcagccgac cacaagtttg gcatcagaaa
     1441 tcattacggg tcgatagggg agaaggaaac gttctttcaa gcggcgaata cgtgcgagag
     1501 actgggccaa ccgtccggca gagatgtccc cgtttgctac ggctttgtca acggcattca
     1561 tcgccgcgac gactcggttg cggtctttac agatcaatgg catgtcgcaa ccggcttgga
     1621 tggcctgtat cgtcgcgtcg ccgatgccat aatggtcaat gatggcatgc atctcgagat
     1681 catccgtcaa cacgacacca tcgtagtgca actcttcacg aaggaatctt ccaatgatcg
     1741 tcggggacag ggttgccggc cggttctcat ccaaagcctg atacaaaacg tgggccgtca
     1801 tcatcgttgc cacgccgttt gcaatcgcgc gtcggaac
//
LOCUS       DASTWE010000813         1454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1444274, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000813 DASTWE010000000
VERSION     DASTWE010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444274"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1310)
                     /locus_tag="VFT30_08445"
     CDS             complement(84..1310)
                     /locus_tag="VFT30_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092701.1"
                     /translation="MAPQSTPYQSDTASLQRLTQFAQDMGHPTSLPVITERILSGLSE
                     VSQHSQGVLYLFDRERECFCQVVSQVQIPLIAFPVFPLNHPLVQRLAHHQGILSSSAL
                     TDSQLATLHIHLAIPLLNRGKLIAFVLLQSQLGTTVISSHTMELLAAMAQNAANALDF
                     FLLYEDLRKSQALMRRTDRLRSLETIAGGFAHEIRNPLTSIKTFIQLAPERKDDSQFI
                     GEFSRIVLDDVNRIERLIQEILDYARYMEPRLTDEDLNEIVSSCLYFIQSKADSRGIK
                     IEKDLAPELPRGMLDRQQIKQVLLNLLLNAMDAMSEKTGTLRVRTTRFQKADSEVWTQ
                     IEIEDTGHGISPENLDHIFDPFFTTKYASAVNEGTGLGLTIAHQIIREHRGEIQVQST
                     EGIGTTFRINLPSHQG"
BASE COUNT          342 a          332 c          413 g          367 t
ORIGIN      
        1 gagaggcacg gactcgtgga tcgtcatcga tcaacagaat tcgtttcctg acaaccgagc
       61 tttccacaag cgctccttgc gttctaaccc tgatgggagg gaagattgat gcggaaggtc
      121 gttccgatcc cttccgtact ctgaacttgg atttctcctc gatgctcccg aataatctga
      181 tgagcgatgg tcagtcccag ccctgttcct tcgtttacgg cgctcgcata tttggtggta
      241 aaaaacgggt caaaaatatg atcgaggttt tcgggtgaga tgccgtgccc cgtatcttcg
      301 atctctatct gagtccacac ctcactatcc gctttctgga acctggtggt ccgtactcgg
      361 agggttccgg ttttttcgct catggcatcc atcgcattca agagcaggtt taaaaggacc
      421 tgtttgattt gttgccgatc taacatccca cgaggaagct caggcgccaa atctttttca
      481 atcttgatcc cgcggctgtc ggctttcgat tggatgaagt ataggcagga cgagacaatt
      541 tcattgagat cctcgtcggt caaccgaggt tccatgtacc gagcgtaatc gagaatttcc
      601 tgaatcaacc gttcaattcg attgacgtca tcgagcacaa ttcggctgaa ttcaccaata
      661 aattgactgt catccttccg ttccggagcc agctgaatga aggtcttaat cgacgtcaag
      721 gggtttctga tttcgtgagc aaaacctccg gcaatggttt ccaaagaacg caatcgatcg
      781 gttcgtcgca tgagggcttg ggatttccgg agatcctcat acagcaggaa aaagtcgagc
      841 gcgttggcgg cattctgggc catagcagcc aagagttcca tggtatggga ggagatgaca
      901 gtggtaccta actgggattg aagcaatacg aaggcgatca actttcctcg attcagcaac
      961 gggatggcaa gatggatgtg aagagtggcg agctgggaat cggtcaatgc ggacgagctg
     1021 aggatgcctt ggtggtgagc cagtcgctga acaaggggat gattgagagg aaagacggga
     1081 aatgctatca aagggatctg cacttgtgaa accacctggc aaaaacattc gcgttcgcga
     1141 tcaaacaggt atagaacccc ttgagagtgc tgggatactt cggagaggcc ggaaaggatg
     1201 cgttctgtga tgacggggag gcttgttggg tgccccatat cctgcgcgaa ctgcgtgagt
     1261 cgttgcagtg atgccgtgtc tgattgatat ggggtcgatt gcggagccat gcgatccatc
     1321 atgagtgtgg tggcgaggtc ttatgtgcct atgtagttcg tagtggtgga aacacggcta
     1381 cgtatcatcc ctaggtggaa agtatacacc cgaggggctg tttcagccag cggatttggt
     1441 cttccgtaca atgg
//
LOCUS       DASTWE010000814         1268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_507025, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000814 DASTWE010000000
VERSION     DASTWE010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507025"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..666)
                     /locus_tag="VFT30_08450"
     CDS             complement(190..666)
                     /locus_tag="VFT30_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092702.1"
                     /translation="MKKRSADSERIASDIVAAAVTRQFTSGDAWDYGWSVLEYRRSTG
                     AAIQHDSNRSVSLSPEGVEAFERAMDQLSKRSDVRNLYDEKELWSIVASLIGTLPMSA
                     PIETIERLVRGRLTDLTAPPNSVVVTSLANVDPGDSPIEAGPLLIGRLGDQWRDRL"
BASE COUNT          291 a          371 c          314 g          292 t
ORIGIN      
        1 cggttccaga accagagcca gcgctattag attctcgaat gcttcctcgg tttcaatgac
       61 agcacggttc gaccgggatt ctccgaaatg tgcgagcaga acgacagcgc gctcgtcaga
      121 accgtcgccc aacgattccc accaaggctt ggttctcgct gaaatcagct cttgccctct
      181 tgtccgagcc tacaaccggt cacgccactg atctccgagg cgcccgatta gcaggggacc
      241 ggcctcgatc ggcgaatctc ccggatccac atttgccaga gaagttacga ctactgagtt
      301 gggtggcgcc gtaagatcag ttaaccgtcc acgaacgagc cgttcaatag tttcaatcgg
      361 cgctgacatg ggcaacgttc cgatcaagct ggcaacgatg ctccagagtt ctttctcatc
      421 gtacagattt cggacgtcgc tccgtttaga cagttggtcc atggcacgct cgaaagcctc
      481 aactccttcc ggactgagac taacggagcg gttgctatcg tgctggatag ctgcaccggt
      541 cgaacgtcga tattcgagaa ccgaccatcc gtaatcccaa gcatcgcccg atgtgaactg
      601 acgagttacg gccgcggcga caatatcgct cgcgatacgt tcagagtctg cagatctttt
      661 tttcacgaca ggcctcccct gcggacaaac atactctttt agcttcaacg ctgaaaagat
      721 tgactttccg gtattgaggt gatctggcaa cctgccggag ctaacctcga agatcatgtt
      781 gcccactctc aaactttggc gtcctgttct ccatttgctc ccgctcgtaa cgcacagttg
      841 cgcgtcacga agcgcacgct ttctccagtg ctaagttctg atactaatca aaggtggcgt
      901 gtgtcactca cgtaatgagc atagctttgc gtcaatcgcc aacgggcaca ggtggccggg
      961 atctccagat gatccggata tgcagtctct gctatcctta ggaccacaga attctttgaa
     1021 attccgtacg tgatgcacag ttcgctcgcc aatatagtga cgaggcatga tcgtcgctga
     1081 tgagtatgga cgatcaatac tcacgacgaa ccattacgac agctatttgc catccgtaac
     1141 aacggatagc ccaaccagcg cgacttcctt ctcccttgga ggtcggggct gatgggccac
     1201 gcacgccggc ggccggaacg gctcgccgag aaactactaa aaattcgaag tacgctcggt
     1261 ctctctca
//
LOCUS       DASTWE010000815         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1412256, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000815 DASTWE010000000
VERSION     DASTWE010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412256"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFT30_08455"
     CDS             complement(<1..987)
                     /locus_tag="VFT30_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5092703.1"
                     /translation="MQQAWVSRYDSGVPSSLNYPGWTVPDLLRHSSDRFANSPALLFY
                     GTWISYGELHDLTTRFALGLRQLGVSQGDRVAIMLPNIPQTVIAYYGIQKAGAVVVPI
                     NPLYVEREIQTQLADAGTETMVVLDLLYPRVRAVKEAIPLPKRIIVTGVQDYLPFFKK
                     LLYPIKARLAKRWVSVEKAPSVSDFLELLRASSPIDADPSAGLPHVQPADLAQLQYTG
                     GTTGTPKGVMLTHRNVVVNTLQGRYWSPDFREGQEIFLGAIPFFHCYGQATCQNLAIA
                     TGCPIVLLPRFHADEVIKAIHKHRITMLSGVPMMYSMIVEHPDAGRYDLRSLR"
     gene            complement(1004..1741)
                     /locus_tag="VFT30_08460"
     CDS             complement(1004..1741)
                     /locus_tag="VFT30_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEU5092704.1"
                     /translation="MTFHQEMRRLVLKHGAINNSYLDRFRVGHLTDSDLKEFALEFYN
                     FSRFFPRILMSQLVNTEDERVADELTKVLYSELGDGQARHRHELLYRNFLRSVGIDIH
                     EAMTRPMLPSTRAYIEGMERLYTDGNHAKALGASFGLENMAITMWDHLIPGLTHLKLT
                     RYPEMDLAYFTFHRQLEVTHEEAMEHAVAAVQGMDETRMSKQDEDDFRFGVDAVLGYL
                     EGFWAGLEQRKTRSDNTWTGLVQRRAS"
     gene            1996..2583
                     /locus_tag="VFT30_08465"
     CDS             1996..2583
                     /locus_tag="VFT30_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5092705.1"
                     /translation="MKQTTGFDHVTLCVDNLEAAEFLFAKVLGFEVIWSARDVGSNTS
                     SMDTVVVQRGTAKIALMQGRDKAGKSQINEFIEKYGQGVQHVAFEVEDIDAVCREWEA
                     HGVKFSGPIKEGMDGFGTLKQRFTYPLFPNAGLFIELTQRQHGEEAAKTFVRSTVESL
                     YKDIERDQTSGVQKTIIDYDSSSMFRPKKPLKKAS"
     gene            2615..3721
                     /locus_tag="VFT30_08470"
     CDS             2615..3721
                     /locus_tag="VFT30_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU5092706.1"
                     /translation="MYLKRRGKVPNQAHVGIPEGLYEEEHGRNGFAGSVSHLYRTHPP
                     TAWTTIEGKLKPRAFACTQIPETVLSSTTQSVLMMQSRDVSLYISCRRETMPYFVRNA
                     DGDEVHFVHRGRGRFETDYGVLSYEPGDYVVIPKATTYRIHVDTEHFFSLTVETPIPM
                     SMPDRGPLGQHALFDKGVLLAPELEAVEPAETGFQTWEVRIKRQGEWTKVGYPFYPMD
                     VVGWKGDLWVAKLNVRDFRPVVSPRYHLPPSVHQTFQAGGCLISTFAPRPLESDPEAL
                     RVPFYHRNTDYDEVLFYHDGEFFSRAGIHAGMLTLHPQGIHHGPQPQAVQAGKAKMYT
                     SEMAVMIESKAPFTVQPEMEPVEIKDYALSWSRR"
BASE COUNT          915 a          952 c         1066 g          839 t
ORIGIN      
        1 gcgcaacgaa cgtaagtcat aacgtccggc atccggatgt tcgacaatca tcgagtacat
       61 catgggcaca cccgagagca tggtaatgcg atgtttatga atcgctttga tgacctcgtc
      121 cgcatggaac cgagggagga ggactattgg acagcctgtc gcgatggcga ggttctgaca
      181 ggtggcttga ccgtagcagt ggaaaaatgg aatagcaccc agaaagattt cctgcccttc
      241 acgaaaatcg ggtgaccaat atcggccctg cagcgtattg acgaccacat tacggtgcgt
      301 gagcatcacg ccttttggcg tgcccgtcgt cccaccggtg tattgtagtt gggcgagatc
      361 ggctggttgc acgtgaggaa ggccggcgga aggatccgca tcgatcgggg agcttgcgcg
      421 caagagttcg aggaagtcgg acacggaagg ggctttctcg acggaaaccc aacgcttggc
      481 taaccgagcc ttgatgggat agaggagttt cttgaagaag gggagatagt cctgaacgcc
      541 ggtgacgatg atccgcttgg ggagcggaat cgcctccttg accgctcgaa cgcgaggata
      601 gagtagatca aggaccacca tcgtctccgt ccctgcatcg gccagctgcg tctgaatttc
      661 tcgttcgacg tagagcggat taatcggcac cacgacagcg ccggcttttt gaattccgta
      721 ataggcgata actgtctgcg ggatattggg aagcatgatg gcgactcggt cgccttgact
      781 gacaccgagt tgtcgcaatc caagcgcaaa tcgagtcgtg aggtcgtgca gttccccata
      841 ggagatccag gtaccgtaaa acagaagtgc gggggagttc gcgaaacggt ccgatgagtg
      901 tcgcaggaga tccggtacag tccagccagg atagttgagg cttgagggca ccccggaatc
      961 gtatcggctg acccaagcct gctgcatagg acctccagcg gcgctaactc gcacgccgct
     1021 gaactaaacc agtccacgtg ttgtcagatc tcgtcttacg ttgctccaaa cctgcccaga
     1081 acccttccag atagccgagt accgcatcga cgccgaaacg aaaatcatct tcatcctgtt
     1141 tggacatcct ggtctcgtcc attccttgca cggccgctac cgcgtgttcc attgcctcct
     1201 cgtgagtgac ctcaagttgc cggtgaaacg tgaaataggc gaggtccatc tcgggataac
     1261 gcgtgagttt caggtgggtc agacccggaa tcaaatgatc ccacatcgtg atggccatgt
     1321 tctcgagtcc aaaggatgct cccagcgcct tggcgtgatt gccgtcggtg tagagccgtt
     1381 ccatcccttc tatatacgcg cgggtcgacg ggagcatcgg tcgagtcatg gcttcgtgta
     1441 tgtcgatacc cacggatcgc aggaagtttc gataaaggag ttcatgcctg tgacgggctt
     1501 gtccgtcgcc caattctgaa tacaggacct tggtcagttc atcagccacg cgctcgtcct
     1561 cggtatttac aagttgagac atcaggatac gggggaaaaa ccgtgaaaag ttgtagaact
     1621 cgagtgcgaa ttccttgagg tcagagtcgg tgagatgtcc tactcggaag cgatccaggt
     1681 aagaattgtt gatggcacca tgcttgagca ccagtcgtct catttcttga tgaaacgtca
     1741 tggccgacct ccagatgtcc tcatgatgat aatttgacaa gcacataata tagtttttat
     1801 attgcaatgg caatagaccg atgattcgtt aacggatcaa ttaacatgcg atgggaggca
     1861 caatttatgc caacttaatt ctgcaagtcg tcttgcaatg aactttgaat gatagtatta
     1921 tcacatcaca cgataataga atataattca tgcgagtggt cttcgtggaa agatataggc
     1981 gatgtggagg gcttcatgaa acagacaacc ggttttgatc acgtcaccct ctgtgtcgac
     2041 aatcttgaag ctgcagaatt cctgttcgcc aaggtcttgg gctttgaagt gatctggtca
     2101 gctagagatg tgggaagtaa cacctcatcg atggacactg tcgtcgtcca acgtggaacc
     2161 gccaagatcg ccctcatgca gggtcgggac aaggcgggga agtcccaaat caacgaattc
     2221 atagaaaagt atgggcaagg cgtgcagcac gttgctttcg aagtcgaaga cattgatgcc
     2281 gtgtgtcgcg agtgggaggc gcatggcgtg aaatttagcg gaccgatcaa ggaagggatg
     2341 gacggattcg ggacgctcaa gcaacgcttc acctatcccc tcttccctaa cgccggactt
     2401 ttcattgagc tgacccaacg gcagcatgga gaagaagctg cgaagacgtt tgtccgaagt
     2461 acggtggagt ctctgtataa ggatatcgaa cgagaccaaa cttccggggt ccagaagacc
     2521 atcattgatt acgattcatc ttcgatgttc aggccaaaga agccgctgaa aaaggcatcg
     2581 taaccgcgcg atccactctt tgtgggagcc ggccatgtat ctcaagcgga gaggaaaagt
     2641 tcccaaccag gcccatgtcg gaattcccga agggctgtat gaagaagagc atggacgaaa
     2701 cggctttgcg ggttcggttt ctcatctcta ccggacacat cccccgacag cgtggaccac
     2761 tatcgaaggg aagctgaagc cgcgggcttt tgcctgcaca cagattcccg agaccgtatt
     2821 atccagtacc acgcaatcag tgttgatgat gcaaagccgg gatgtaagtc tctacatttc
     2881 ctgccgacga gaaacgatgc cctatttcgt gcgcaatgca gatggagatg aagtccattt
     2941 cgtgcatcgg ggcagaggca ggtttgagac cgattatggc gtgttgtcct acgagccagg
     3001 tgactacgta gtgatcccca aagcgacgac ctatcgaatt cacgtcgata cggaacattt
     3061 tttttcgctg acagtggaga cacccattcc tatgtcgatg ccggatcgag gccccctcgg
     3121 acagcatgcg ctgttcgata agggcgtgtt gcttgctcct gagctggagg cagtagaacc
     3181 ggcggaaaca ggattccaaa catgggaggt ccgcatcaaa cgtcaaggtg agtggacgaa
     3241 agtgggctat ccgttctatc ccatggatgt agtcgggtgg aaaggcgatc tgtgggttgc
     3301 gaagctgaac gtgcgggact tccggcccgt cgtcagtccc cgttaccatc tgccgccgag
     3361 cgttcatcag acgttccaag ccggaggatg cctcatttcc acttttgccc cgaggccgtt
     3421 ggaaagtgat ccggaggcct tgcgcgtgcc cttttaccat cgaaataccg actacgatga
     3481 agtgctgttc tatcacgatg gagaattctt cagccgtgca gggattcacg cggggatgct
     3541 tacgttacac ccgcagggta tccaccacgg accacaaccg caggcggtcc aagccggcaa
     3601 agcgaagatg tacaccagcg aaatggcggt gatgatcgaa tccaaggctc ccttcacggt
     3661 ccaaccggag atggagccgg tggagatcaa ggactacgca ttgagttgga gccgtcgatg
     3721 aagctcgtca gttttcaaat cagaactccc atcggtacct tcacaagggt gg
//
LOCUS       DASTWE010000816         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1573349, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000816 DASTWE010000000
VERSION     DASTWE010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573349"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1062)
                     /locus_tag="VFT30_08475"
     CDS             complement(298..1062)
                     /locus_tag="VFT30_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092707.1"
                     /translation="MRDWKGALQWYRTHQTAGQIGFDPDGMCLKVCRTARDIPARYLT
                     ARQAQDATPREHRVERVADLRRGMVGYFDDPNDSNTAGHIATMVGRVKGADVNDLNDT
                     LWETNSVKANELVVVRGSYFAVHWGDRFYFGATWLNGFELDVPAKKIAESLGAARIEN
                     FRESRPEWDVKILDRLVNSNRGDLRQLVNRIEQAVDNLPDDLKDTRVKEFKEIFNKRR
                     VLKMSLLNQAVQDGRLARVKTERDRLNMLIKTVLRK"
     gene            complement(1055..1276)
                     /locus_tag="VFT30_08480"
     CDS             complement(1055..1276)
                     /locus_tag="VFT30_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092708.1"
                     /translation="MREYDPEVEEVDLEDVNLYEGKPDFEASPAKQADQLGNQDELEQ
                     GEALGDDLAPAQTKPVDRDVAEEQEDPDA"
     gene            complement(1286..2044)
                     /locus_tag="VFT30_08485"
     CDS             complement(1286..2044)
                     /locus_tag="VFT30_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092709.1"
                     /translation="MDIFQLRMQHSSLQFSDKPRQQRRDIRDLFALGPRFPVKSGTEA
                     GKSSTNRNREYLKHFAELYEHRIHFAADNWVAVHRDIIRPNSWDTGHVFAVDNAETKG
                     RGHDTVMATVHFDHVEDGFGEVNIGAVHYPTQGRTPKDPNWDENKQAAEIFGAWLEEV
                     AQGSALGFINGDFNMADPIADWAFGENFTSMADELGDYEGTGHGPIDGFTSYDRDGRV
                     SAAAFRVLDDREFFQHSDHFVCRGVWNVRSLNAA"
     gene            complement(2046..2471)
                     /locus_tag="VFT30_08490"
     CDS             complement(2046..2471)
                     /locus_tag="VFT30_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092710.1"
                     /translation="MTERLLTYSELVKRTTLLARFKYAVLGGSLGVRTFGFDRDLNQA
                     FYGSYMWKRARDEVLVRDEGFDLGVAGYPIMRNPHVHHMNPLTVDDFVNYDEDRLFNP
                     EYLITVSHRTHNAIHFGDESQLPQDFVERRPGDHLLWRE"
     gene            complement(2464..2712)
                     /locus_tag="VFT30_08495"
     CDS             complement(2464..2712)
                     /locus_tag="VFT30_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092711.1"
                     /translation="MQQLGELAEDLLEERVRKGTASPTESVALLRANSPIEIANLERI
                     RAQTAYLKAQEEKARSETFREEMMTKAIEAMKRYNPHD"
     gene            complement(2943..>3161)
                     /locus_tag="VFT30_08500"
     CDS             complement(2943..>3161)
                     /locus_tag="VFT30_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092712.1"
                     /translation="TNPYDPFTEYGDWLRFDHHEGFDTAGLLARALSTADALGDPDQE
                     LAVEQAIESSLSNPSFVGLYKKVEREVT"
BASE COUNT          618 a          937 c          894 g          712 t
ORIGIN      
        1 ttcgcatcca tttgatggaa tccgggtatt cgtaccggtg acccatccca acgccgatga
       61 cgatggcgaa gggagaggcc acaagtacgc tggcccagat catgaccacc cactcgggca
      121 aggcctcctc catgctccca gcttcaatac cactgaatag cagagtgagc cctccgagaa
      181 cgcaaaggaa agctgtgaaa agctcaaagt cacgctctcg gatccacctg ggtatccatt
      241 ggtacacctt acgccgaatc ttagagattc ggcccgggag cttgcggtga ggcattgcta
      301 cttccgcaag acggtcttga tcagcatgtt gagacggtcg cgctcggtct tcacacgagc
      361 cagacgtccg tcctggacag cctgattgag cagcgacatc ttcaggacgc gccgcttgtt
      421 gaagatctcc ttgaactcct tgacccgagt gtccttgagg tcgtccggga gattgtcgac
      481 cgcctgctcg atgcgattga cgagctgacg aaggtccccc cggttggagt tgacgagtcg
      541 atccaggatc ttgacgtccc actccggtcg agactcacgg aagttctcga tgcgcgcagc
      601 accgagcgat tccgcaatct tcttggccgg gacatccagc tcgaagccgt tgagccaggt
      661 ggcaccgaag tagaaacggt caccccagtg aaccgcgaag taggatccgc ggaccacgac
      721 cagctcgttt gccttgacgc tgttggtctc ccacagcgtg tcgttgagat cgttgacgtc
      781 ggctcccttg acgcggccga ccatcgttgc gatgtggcca gcggtgttgg agtcgttggg
      841 atcgtcgaag tagccgacca tcccacggcg aaggtcagcg acccgttcga cgcgatgctc
      901 tcgaggcgtt gcgtcctggg cctgccttgc ggtgaggtat cgagcaggga tgtcgcgcgc
      961 cgttcggcag accttcagac acatgccgtc gggatcgaag ccgatctgac ctgcggtctg
     1021 gtgcgtgcga taccactgaa gcgctccctt ccagtcacgc atcgggatcc tcctgctctt
     1081 ctgcaacgtc acgatcgacg ggcttcgtct gggccggcgc caggtcgtct ccgagggcct
     1141 cgccctgctc gagctcgtcc tggttgccca gctgatcggc ctgcttggcg ggagaggcct
     1201 caaaatcagg cttcccttcg tacaggttca cgtcttcgag atcgacttcc tcgacctcgg
     1261 ggtcgtactc tcgcattcgg cctcctcagg ctgcgttcag ggaccggacg ttccacacgc
     1321 cacggcagac gaagtgatca ctgtgctgga agaactcccg gtcgtcgagg acacggaacg
     1381 cagcagcaga aacgcggcca tcacgatcgt aggacgtgaa tccatcgatc ggcccatggc
     1441 ccgtgccttc gtaatcgccg agctcgtcgg ccatcgacgt gaagttctcg ccgaaagccc
     1501 aatccgcgat cgggtcggcc atgttgaagt cgccgttgat gaagccgagg gctgagccct
     1561 gggcgacttc ctcgagccag gcaccgaaga tctcagcggc ctgcttgttc tcgtcccagt
     1621 tgggatcctt gggggtgcga ccctgcgtcg ggtagtgaac ggcaccgatg ttgacctctc
     1681 cgaagccgtc ttcgacgtga tcgaagtgca cggtcgccat cacggtgtcg tgaccgcgtc
     1741 ccttggtctc cgcgttgtcg actgcgaaga cgtgacccgt gtcccaggaa ttgggtcgaa
     1801 tgatgtcgcg gtgaacggcg acccagttgt ccgctgcgaa gtgaatccgg tgttcgtaga
     1861 gctcagcgaa gtgcttgagg tactcccggt tgcggttggt cgagctcttg ccggcctcgg
     1921 tgccggactt gactggaaag cgcgggccaa gagcaaagag atcgcgaata tcacgtctct
     1981 gttggcgcgg cttgtcgctg aattgcagcg atgaatgctg catcctcagc tgaaagatgt
     2041 ccatactact ccctccagag taggtggtcc cccggccgtc tctcaacaaa gtcctggggt
     2101 agttggcttt catcaccgaa atgaatggcg ttatgagtcc tgtgagacac cgttatgagg
     2161 tactcagggt tgaaaagacg gtcttcgtcg tagttaacga agtcgtcgac cgtcaacgga
     2221 ttcatatggt gcacatgtgg gttccgcatg atcggataac ccgcaacacc gagatcaaag
     2281 ccctcatcac ggactaagac ctcgtcgcga gctcgtttcc acatgtagct gccataaaag
     2341 gcttgattga ggtctctgtc gaacccaaag gttcggaccc ccaacgaacc gcccaaaacg
     2401 gcgtacttga accgagctag gagcgttgtt cgcttcacca attcggaata ggtcaagagt
     2461 ctctcagtca tgagggttgt acctcttcat ggcctcgatc gccttagtca tcatctcctc
     2521 gcggaaggtt tctgacctgg ctttctcttc ttgtgctttg aggtatgccg tctgtgctcg
     2581 gatccgttcg agattcgcga tctcaatcgg actattggct ctgagaagag cgacagactc
     2641 cgtgggagaa gccgtgccct tccgaactct ctcttcgagc agatcttcag ctagttcacc
     2701 tagctgttgc atccgtctgg ccctggttct cgccggcggc cgacgctttg gtacctcttc
     2761 cggtgacctt tcaggtgaac tggaagactt tggcacgact ttcaccccct ttcaatctag
     2821 cctccgggtc tttccctagg gttaatagtc gatttcgacc ttccccccgg agaaatatcg
     2881 aggagggcgg cgatggagaa ggggggtata ttttttcgac ccccctcccc ccatcaaaag
     2941 tttcaggtga cttctctctc gaccttcttg tacaacccca cgaagctagg attgctcaga
     3001 ctggattcaa tcgcttgttc cactgccaac tcttgatctg gatcacccag cgcatcagca
     3061 gttgagagcg cgcgagccaa caaccctgct gtgtcgaatc cttcatgatg atcgaatctc
     3121 aaccaatcac catactccgt gaagggatcg taggggttgg t
//
LOCUS       DASTWE010000817         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_474815, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000817 DASTWE010000000
VERSION     DASTWE010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474815"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..170
                     /locus_tag="VFT30_08505"
     CDS             6..170
                     /locus_tag="VFT30_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092713.1"
                     /translation="MGLTEAGYTDLTNIVAGIAKRHAQGRILSSLEGGYNLTALARSV
                     DAHINALLNA"
     gene            207..1085
                     /locus_tag="VFT30_08510"
     CDS             207..1085
                     /locus_tag="VFT30_08510"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5092714.1"
                     /translation="MKHPLLIDTETLQQHLGHPDLVVIDVRGKAAYEFGGHIPGAVHS
                     TWHEYSDPNAVPKGLLNPDLGQLERILRRLGMNQDSDVVIYSNPFDNWGDEGRMFWML
                     EYLGHKRLRILDGGWVKWTAEKRPFEHGPVSPMMGNFKAQPVRTLIALKDDLKGLVRG
                     PHPQTAILDARSVEEYLGKEVSGLPRSGHIPSAIHVPWNGFLNKDATVKDLSVIKEML
                     EAKGVRSEQEVICYCTGGVRSAWLYFILKLVEYPNISNYPGSWWEWSRDFACPAEKDL
                     HALQKILGFDPASKPS"
     gene            1169..1513
                     /gene="smbP"
                     /locus_tag="VFT30_08515"
     CDS             1169..1513
                     /gene="smbP"
                     /locus_tag="VFT30_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HEU5092715.1"
                     /translation="MMRRILRSAMLVLGLGVLVSAPVLNGSALAADKHAAEALEHAKA
                     AVSHGKEGHADACVEHASEALKHAQAAAKNPHGAEGVKHLTEAVKHGKAGHADACTEH
                     AEGAATHLAEVK"
BASE COUNT          421 a          437 c          529 g          380 t
ORIGIN      
        1 caagcatggg acttacggag gctgggtaca ctgacctgac gaatatcgta gcgggtattg
       61 caaaacgtca cgcacagggg cgcatccttt cctccttgga gggcggctac aatctgactg
      121 cgttagctcg ctcagtggat gcgcacatca acgcgttatt gaatgcttga atcactctac
      181 tgcgaactgt aaagaaagaa gatatcatga agcatccgct attgattgac accgaaacgc
      241 ttcagcagca cttaggacat cctgatcttg tcgttatcga tgtccgcggc aaggcggcgt
      301 acgaattcgg cggacatatc cccggagccg tgcattcaac atggcacgaa tacagtgatc
      361 ccaatgccgt gccgaaggga ttgctcaacc ccgatctcgg ccagcttgaa cgtatcctga
      421 gacggctcgg gatgaatcaa gatagtgatg tcgtgatcta ctccaatcca ttcgacaatt
      481 ggggcgatga agggcggatg ttttggatgt tggaatatct cggccacaaa cgcctgcgta
      541 ttttggatgg cgggtgggtg aagtggacgg ccgagaaacg gccgttcgag catgggccgg
      601 tctctccaat gatgggaaat ttcaaggctc aaccggtgcg gactctgatc gcgttgaagg
      661 acgacctgaa gggcctcgtc agggggccgc atccccagac ggccattctg gatgctcgca
      721 gcgttgaaga atatcttggg aaagaggtgt ccggtctccc gcggtccggt cacatcccct
      781 cagccataca cgtgccatgg aatggcttct tgaacaagga cgcgaccgtc aaggatctat
      841 ccgtaattaa ggagatgttg gaggcaaagg gggtacggag cgaacaggaa gtcatttgct
      901 actgtaccgg cggtgtgcga tcggcttggc tctatttcat cctcaagctg gtcgagtacc
      961 cgaatatcag caactacccc ggatcgtggt gggaatggag tcgggacttc gcttgtccag
     1021 cggaaaaaga tcttcatgcg ctccagaaaa tcctcggatt tgatccagcc tccaaacctt
     1081 cttgacagtc tggggagcgc tgtgtaaggt gaattcacaa aatttcttca cttacaactg
     1141 cgaatcttca accatcaagg aggcagccat gatgagaaga atccttcgta gtgcgatgtt
     1201 ggtactcggc cttggcgtgt tggtcagcgc cccggtactg aatggttcag cgctcgccgc
     1261 cgataagcat gcggctgaag cacttgaaca cgcgaaggcc gccgtgtcgc atgggaaaga
     1321 aggccatgca gatgcttgtg ttgagcatgc gagtgaagcg ctcaaacacg cgcaggcggc
     1381 agcgaaaaac ccacatgggg cggaaggcgt gaaacactta acggaagccg taaaacatgg
     1441 taaagccggg catgccgatg cctgtaccga acatgccgag ggggcggcca cgcatctggc
     1501 tgaagtgaag tagtcgacga gaagcagcca cggctgcagt aagttgcttt tgttgaagcg
     1561 ggcagggaca taatccctgc ccgttcttgt atggagggag tgcgtggtgc gagtcgggtt
     1621 ttaccaattc gatccacagt ttggtgaggt ggccaagaat ctggagatgg tcacggccaa
     1681 gctggaacag gcggacgcgg acctcatcgt gttgccggaa ttgttcgcgt ccggctatca
     1741 gtttctttca cgggaggaag cgcaaca
//
LOCUS       DASTWE010000818         1345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_54470, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000818 DASTWE010000000
VERSION     DASTWE010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54470"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFT30_08520"
     CDS             complement(<1..1193)
                     /locus_tag="VFT30_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5092716.1"
                     /translation="MPENGDLEGVNEPVITLNAEIDWDDEAEVVVVGSGIAGCSVAIN
                     CAVQGASVLVLEKSGSTGGTSAKSGAGALVPNNRFLRQAGRETESKADFIGLLARLGR
                     PALYDTSHPTFGLPQWEYDLIETYYDNAEEAWSYMEDIDAVKVMHFPDWPSYNDIPEE
                     KYPFGRMIFPCDDDGQLAFGREAIDRLVRKATALGAKLLTGFGVTGVYVNQVGEIVGV
                     RGFSDGEVRSVRALRSVVFSTGGFTHSSQYAREYLNGQIVGGCAARTNTGDFIPIAKA
                     LGAPLFQMNSAFLAPLVYEQAIDKDPDLVTNFNFAGDSGLVVNKYGVRVGNEKAQYND
                     RTQSHYVWDAHRAEYPNFLQFGIIDQRTRENFGYSGRGDYQAGNFIPSPGESSKYLIE
                     AETIP"
BASE COUNT          270 a          420 c          355 g          300 t
ORIGIN      
        1 tctggaatcg tctccgcctc aatgaggtac ttggacgact cacctgggct cgggatgaag
       61 ttcccagctt ggtagtcgcc gcgtcccgag tatccgaagt tctctcgagt tcgttgatca
      121 atgatcccga actgcagaaa attcgggtac tccgctcgat gcgcatccca gacatagtga
      181 gactgcgtcc gatcgttgta ctgggccttc tcgttcccga ccctgactcc gtacttgttg
      241 acgacgagac cactgtcgcc ggcgaagttg aagttcgtca ccagatcggg atctttgtcg
      301 atggcctgct cgtacacaag gggcgcaagg aaggccgagt tcatctggaa cagaggagca
      361 cccaatgctt ttgcgatcgg gatgaagtca ccggtgttgg ttcgagcggc gcatccgccc
      421 acgatctgac cgttgagata ttctctcgcg tactggctgc tgtgcgtgaa accaccggtc
      481 gagaacacca cggaccgcaa ggcgcgtacc gaccggactt ctccatcaga gaatccacga
      541 acccctacga tctcgccgac ttggttcacg tagactcccg tgacaccgaa tccggtcagc
      601 agcttcgccc ccagagcagt cgccttcctc accagtcggt cgatcgcttc acgaccgaag
      661 gcaagctgac catcgtcatc gcagggaaat atcatcctcc cgaatgggta cttttcctcc
      721 gggatgtcgt tgtagctcgg ccaatcagga aaatgcatca ccttcaccgc atcgatgtcc
      781 tccatgtacg accacgcttc ttctgcgttg tcgtagtagg tctcgatgag gtcgtactcc
      841 cattgtggga ggccgaacgt cgggtgcgac gtgtcgtaga gggcggggcg gccaagtcgt
      901 gcgagcagcc cgatgaagtc ggctttggac tctgtttccc gtccggcctg acgaagaaac
      961 cggttgttcg gaaccaacgc tccagcacca gacttcgcgc tggtcccacc ggtggagcca
     1021 ctcttctcca gcacgagcac cgaagcgccc tgcacagcac agttgatggc cacagaacaa
     1081 cccgctatcc cagatccgac caccaccacc tccgcctcgt cgtcccagtc gatctccgca
     1141 ttcaatgtga tgaccggctc attgacgcct tctagatcgc cattttcagg catgctttcc
     1201 tctcgcttgg acacagttgg attcggacaa agtcagttgg cgttggtttc gtaggcatcc
     1261 tcgacgaact cctgctccca ggtgatctcg ccgatcacat cagtgcgtcg aaatggccgt
     1321 atgtcatcga tccgattctc tcgga
//
LOCUS       DASTWE010000819         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_959506, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000819 DASTWE010000000
VERSION     DASTWE010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959506"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..737)
                     /locus_tag="VFT30_08525"
     CDS             complement(288..737)
                     /locus_tag="VFT30_08525"
                     /inference="COORDINATES: protein motif:HMM:NF016798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUN14 domain-containing protein"
                     /protein_id="HEU5092717.1"
                     /translation="MSTVPVQAQKSGFLAKTLGTLASDPPWAAKSFLAASATTVAGIG
                     AWLSNMMSPALASAGGSFLGGFLIGWAFRKTLKLALLVTGSLLVLIAILKTTGWIHLD
                     WTLIQADVNHILDWGRGKAQGFKEVLTGYLPSAGAAGAGALFGFRKK"
     gene            complement(734..1594)
                     /locus_tag="VFT30_08530"
     CDS             complement(734..1594)
                     /locus_tag="VFT30_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU5092718.1"
                     /translation="MRIEDQRESDNIEDRRGMGPARIGRGGLGIGTIVLALAVGYFTG
                     VNPLTVLNLLTGVQSMTEVSAPSESGPVGTPQDQLGRFASVVLADTETTWRNLLGPRY
                     EDPRLVLFSGAVHSACGTTSSAVGPFYCPNDHRVYLDLAFFDEMAHRLGATGDFAQAY
                     VIAHEVGHHVQNLLGVAEQVHRLRRQVSEVEANALAVRMELQADCYAGVWGHHAKRER
                     NLIEPGDFEEGHRAAAAIGDDQLQKMSRGHVQPESWTHGSSEQRMSWLKRGLESGDPS
                     VCNTFDTKRL"
BASE COUNT          395 a          480 c          493 g          387 t
ORIGIN      
        1 aataacgtct tacgtgttga tagcgacagt gtgctgtttg ttgtttgtct ttattgtaat
       61 tcggaatctc ggaaagtcac gatcacagca tccgtcaaac tttggagaag aaattaccgc
      121 gggcgagctg aagaattgtg cttcttgaac gagtacattc aagcggaccc agtccgctga
      181 ggattccgct ttcgaatcaa tcttcacaac cttgcgaaca tcaacataac cttgatagct
      241 gattgcggca caaactctga ttgaatccga ggtagttgaa ctagaaatta cttcttccga
      301 aatccaaaca atgctccggc cccggcggcg ccagctgagg ggagatagcc ggtcagcact
      361 tctttaaaac cctgagcttt gcctcgaccc caatcaagaa tatgattcac atctgcttga
      421 attagtgtcc aatccagatg aatccagccg gtcgttttaa ggatggcgat gagcaccagc
      481 aatgagccgg tgaccaacag ggcgagcttc agagttttcc ggaatgccca gccgatcaag
      541 aatcccccga ggaaactgcc acctgcgctg gcgagtgccg gagacatcat attgctgagc
      601 caggcgccaa ttccggcgac ggttgtcgcg ctagcggcga gaaacgattt ggcggcccag
      661 ggagggtcgg acgccagtgt gcccagcgtc ttagctaaaa agccactctt ctgagcttga
      721 acgggtacgg tgctcatagc cgtttcgtgt cgaacgtatt gcagacagag ggatcgccgg
      781 attcgagacc gcgcttgagc caggacattc gctgttcaga tgaaccgtgg gtccagcttt
      841 ccggctggac atggccacgc gacattttct gcaattgatc atcgccgatg gccgccgccg
      901 cgcggtgtcc ttcttcgaaa tcgcccggtt cgatcaagtt tcgttctcgc ttggcgtgat
      961 gaccccagac accggcataa caatcagctt ggagttccat tcgaaccgca agcgcatttg
     1021 cttccacctc agacacctgt cgtcggaggc gatgcacctg ttccgcgacg ccaaggaggt
     1081 tttgtacgtg atgaccgacc tcatgggcga tcacataggc ctgagcaaag tcaccagtcg
     1141 ctccgagacg atgcgccatt tcatcgaaga aggcaagatc caaatagacc cggtgatcgt
     1201 tggggcaata gaatggacca acggccgatg acgtagtgcc gcatgccgag tgcacggcac
     1261 cggaaaagag gaccaggcgt gggtcttcat atcgaggacc gagaaggttt cgccaggtgg
     1321 tttcggtatc tgcgagaacg acggaggcaa acctgccgag ttggtcttga ggggtgccga
     1381 ccgggccgga ttcagatggg gcggatacct cggtcatgct ctggacaccg gtgaggagat
     1441 tcagcacggt caggggattg acgcccgtga agtaaccgac ggcgagcgcc agtacaatgg
     1501 tgccgatgcc gaggccgcct ctcccgatgc gggccgggcc catgccgcga cgatcttcga
     1561 tgttgtcgct ttcgcgttga tcttcgatac gcataggcga cctccctcat gccgcacaat
     1621 cagtccgatt ccttttccat cgactgaatg aatttgtcag cttcggcaat ggaacggttc
     1681 atgtctttga ctagctgatc gacctgcgcg tcgactttca cgagttcccc cttgatcgcg
     1741 gcgagcgcac gcgcg
//
LOCUS       DASTWE010000820         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_636210, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000820 DASTWE010000000
VERSION     DASTWE010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636210"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..>1210)
                     /locus_tag="VFT30_08535"
     CDS             complement(72..>1210)
                     /locus_tag="VFT30_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5092719.1"
                     /translation="HSSPIVWGNRIFLTTAVEGDTVAGAKAVKHTLEGGKEFLHPDSV
                     GADKKHTFKVVSIDRDSGKIVWQATAWEGTPYDNRHRKSSYAASTPATDGKLVYAFFG
                     TEGLYAYDFKGKLAWKADLGKLGTVGMGTGTSPILFDNLVIVQADEENGEASFIVAVD
                     KNTGKEAWRTPRKVQVSWSTPLLVRTSKRAELIASGTEAVISYDPATGKELWRHKGVE
                     SNAIPSPVANNDMVFLVAGFPAKIAMAIKLGQSGDLTGTPNVAWTYAKGTAYVPSPIL
                     YGDYLYLTTDRGILTAVDAKTGEVKYEGGRIPIPATFTASPVAFEGKILMTSEDGDTF
                     IVKAGPKHEVIGTNSVGEPVYASPAIADGRIFIRGEKNLYCIGT"
BASE COUNT          257 a          342 c          324 g          287 t
ORIGIN      
        1 gtcataaaat ccgtcctgaa tccgtctaat ctgtagtttg gtggctacag attagacgga
       61 tggtaacgga tttatgttcc aatgcaatag agattcttct cgccgcggat gaagattcgt
      121 ccatcagcaa tggccgggga ggcgtaaaca ggctcgccaa ccgagttcgt gccgatgact
      181 tcatgcttcg gacctgcctt gacgatgaac gtgtcgccgt cttcgctggt catcaaaatc
      241 ttgccttcga atgcgactgg agacgccgtg aacgtggcgg gaatcgggat tcggccaccc
      301 tcgtatttca cttcgccagt ctttgcatca acagcggtga gaattccgcg atccgttgtc
      361 agataaagat aatcgccgta caggatcggc gagggaacgt acgcagtgcc tttggcatac
      421 gtccaggcta cgttgggagt accggtaaga tcaccgcttt gaccgagttt aatcgccatc
      481 gcgatcttcg ccgggaatcc ggcgacgaga aacaccatat cgttgttagc gacaggcgac
      541 gggatcgcat tgctctccac gcccttgtgc cgccacaact ctttcccggt cgcgggatcg
      601 taagaaatca cggcttcagt tccacttgcg atcaactccg cacgcttcga cgtccgaaca
      661 agcaacggtg tagaccagct cacttgcacc ttacgcggcg ttctccacgc ctccttgccc
      721 gtgttcttgt caacagcaac gatgaacgaa gcctcgccat tttcttcatc agcctgaaca
      781 atcacgagat tgtcgaacag aatcggtgac gtaccggtgc ccatgccgac agtgccgagt
      841 ttgccgagat cagctttcca cgcaagcttg cccttgaagt cataggcgta gagaccttcg
      901 gtgccgaaga acgcatagac cagtttgcca tctgtcgcgg gagtcgaagc ggcataggag
      961 ctcttgcggt gacgattgtc gtagggcgtg ccttcccatg ctgtcgcctg ccagacaatt
     1021 ttgccggaat cgcgatcgat gctgacgact ttgaaagtgt gtttcttatc ggcgccgacg
     1081 ctgtccggat gcaagaactc tttaccacct tcgagggtgt gcttgacggc tttcgcgccc
     1141 gccactgtgt ctccttcgac tgcggtcgtc aggaatatgc ggttgcccca cacaatcggc
     1201 gatgaatgcg
//
LOCUS       DASTWE010000821         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_766091, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000821 DASTWE010000000
VERSION     DASTWE010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766091"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..827)
                     /locus_tag="VFT30_08540"
     CDS             complement(150..827)
                     /locus_tag="VFT30_08540"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5092720.1"
                     /translation="MKNLRAKVWLALVVLAVVMGLLLFVPAGTVHYWQAWVYLSIFTG
                     ASVLTTLYLLRKDRALLERRMSGGPTAEERSVKKFIMLCMSIGFIALLVVPAFDHRFG
                     WSALPLGGVVAGDVLVAIGFYLIFLVYRKNTFTSATIEVAEDQKVISTGPYAIVRHPM
                     YASAFLYLLGTPLALGSYWGLVPIAAMIPLLIWRLFDEEHFLARHLPGYTEYQKRVRH
                     RLVPFVW"
BASE COUNT          270 a          296 c          262 g          213 t
ORIGIN      
        1 gtgccgcatc ccacatccag agcacgagcg accggctcgg caagtgatag atacgctttt
       61 attcggctga ccacttgcgg atgaaaatag ggtcgacctt tcgcatatct ctcagccacg
      121 gatttcggcg caaaatagtt cattctcgac taccacacga atggcacaag acgatgccgg
      181 acccgtttct gatattcggt atatccgggt agatgcctag cgagaaaatg ttcctcgtca
      241 aagagccgcc atattagcaa tggtatcatc gcggcgatcg ggacaagccc ccagtacgaa
      301 ccaagcgcca acggcgtacc gagaaggtac aagaatgcac ttgcgtacat cgggtggcga
      361 acgatggcgt acggcccggt cgaaataacc ttctgatcct cggcaacttc aatagttgct
      421 gacgtgaacg tgttttttcg atacaccaag aaaatcagat aaaaccctat cgcaacgagc
      481 acgtccccgg ccacaacccc gccgagcggc agggcggacc acccgaagcg gtggtcgaac
      541 gctggcacga caagaagggc gataaagccg attgacatac acagcatgat gaactttttc
      601 actgatcgct cctccgccgt cggaccgcca ctcatccgac gctcaagaag tgcccggtct
      661 tttctcagga gatagagcgt agtgaggacc gatgcgcccg tgaagatcga caagtacacc
      721 caggcttgcc aatagtgcac ggtccccgcg gggacgaaga gtagcagccc catcacaaca
      781 gcgaggacga cgagtgcgag ccagaccttt gcacgaaggt tcttcattgc gccatcgccc
      841 gtgacgatag ccgacgtgga tgcaaccgaa ctactgagta taccgccact cctccggtgg
      901 atatctagat atgagtattt tccatcaccc gtaaaaatct ttcatagcat ccaaagtcac
      961 gttcattctg tcaagtattt gttcgctaca aaggctgcca atttggtggt atgttctgat
     1021 gtcagcccac agcatagcgt c
//
LOCUS       DASTWE010000822         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_733068, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000822 DASTWE010000000
VERSION     DASTWE010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733068"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3827
                     /gene="gltB"
                     /locus_tag="VFT30_08545"
     CDS             <1..3827
                     /gene="gltB"
                     /locus_tag="VFT30_08545"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEU5092721.1"
                     /translation="SRFSTNTFPTWPRAHPYRYVCHNGEINTLKGNVNWMKARQGRLH
                     SELFGKDMDKLFPIVSENQSDSACLDNTLEFLLLGGRSLPHAMMMLIPEPWVANAQMD
                     LDRRGFYQYHAAMMEPWDGPAAVCFTDGKMIGATLDRNGLRPCRYQVTTDGLVVLASE
                     AGVLPVEAKDIRMKGRLQPGRMFLVDTVQGRIIDDEEIKADIVGRKPYRSWVTQYGVS
                     LDELPEPLNVPQPDHPTIRQRQQAFGYTVEELKMVITPMIVTGEEAISSMGTDTPLAV
                     LSDRPQLLFKYFKQLFAQVTNPPIDPIREQLVMSLVTNIGPKPNLMDESPESCRRIKV
                     QQPILTNADLQKIRGISDPHFKSKTLRMLFRVAEGPDGLGEAVDELCQQASQAIKEGY
                     KFLILSDRGVDEQWAPIPSLLGISAVHHHLVRECTRTEVGLILETGEPRDVHQFACLI
                     GYGAGTINPYLLFESLVDMERDNYLPEGLDAQTAEGKFIKAINKGLLKIFSKMGISTV
                     QSYCGAQIFEAIGLNRELIDRYFTGTPSRVEGVDIRDIGEETLRRHRVAYEPAAIRQL
                     DFGGEIHYRIQGEHHNWNPETIYKLQHASRSNDPKTYAEFAQIVNDESKRRSNLRGLL
                     DFKFDPQPVPIEEVEPVKEIVKRFNTGAMSFGSISKEAHETLAIAMNRLGGKSNTGEG
                     GEDPERFIPLPNGDSKNSYIKQVASARFGVTAHYLVNARELQIKMAQGAKPGEGGQLP
                     GHKVDENIARFRYATPGVQLISPPPHHDIYSIEDLAQLIFDLKNSNPDAGVSVKLVAE
                     VGVGTVAAGVAKAHADKVLISGDSGGTGASPLASIKYAGIPWELGLAETHQTLVLNDL
                     RGRIRVETDGQMKTGRDVVIATLLGAEEYGFATAPLIVEGCIMMRKCHLNTCPVGIAT
                     QDPELRKKFDGKPEHIVNYLFFVAEEARQLMAKLGFRTINEMVGRVDKLKITKAVDHW
                     KAKGLDLTPLLTAPDVPADVPRYCVQKQDHGLAGILDNKLVELCKASIEQGEKVKLEL
                     PIRNINRTTGTVLSSKIAKKYGPDGLPEDTISIKFTGSAGQSFGAFLAKGITLTLEGE
                     SNDYIGKGLSGGKIIVFPPKNILYDPEETILIGNTSLYGATQGEAYFYGMAGERFAVR
                     NSGAHAIVEGTGDHGCEYMTGGVVVVLGRTGRNFAAGMSGGVAFVLDDAGTFQSRCNT
                     GMVELEPVTTKEDKQLLHGLITKHFMYTGSRKAKQVLDAFEATLPKFAKVMPVDYKRV
                     LEERKRKASAVH"
     gene            3933..>4400
                     /gene="gltD"
                     /locus_tag="VFT30_08550"
     CDS             3933..>4400
                     /gene="gltD"
                     /locus_tag="VFT30_08550"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEU5092722.1"
                     /translation="MGDPKGFMKYAREGPKRKPIELRVLDWKEMYEPISEDKLKAQGA
                     RCMDCGVPFCQGHTGCPVVNLIPEWNDLVYRGRWKDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLAINEDPVSIRVIEWNIVDRGFNDGLVTPILPVVKTGKTVAIVGSGPA"
BASE COUNT          994 a         1202 c         1334 g          870 t
ORIGIN      
        1 actcacgatt cagtacgaat accttcccca cctggccgcg ggcccatccc tatcggtatg
       61 tatgccacaa tggggagatc aatacactga aggggaacgt caattggatg aaggctcgcc
      121 aaggccgtct tcattccgag ctgttcggga aggacatgga taaactgttc ccgatcgtct
      181 ccgagaatca aagcgactcg gcttgtctgg acaataccct cgaatttctg ctgctcgggg
      241 gccgctcgct gccgcatgcc atgatgatgt tgattcccga accgtgggtg gcgaatgccc
      301 agatggattt ggatcgtcgg ggcttctacc agtatcacgc ggcgatgatg gagccatggg
      361 acgggccggc cgccgtctgt ttcaccgacg gcaagatgat cggtgcgacg ttggaccgga
      421 atgggctgcg cccctgccgc tatcaggtga cgacagacgg tctcgtggtc ctggcatccg
      481 aagcgggcgt cttgccggtt gaagcgaaag atatccgcat gaaaggccgg ctccaaccgg
      541 ggcgaatgtt cctcgtggac acggtgcagg gccgtatcat cgatgacgag gagattaagg
      601 ccgatatcgt cggccgcaaa ccttatcgca gttgggtgac gcagtacggt gtttcattgg
      661 acgagttgcc ggagccgctg aatgttccgc aacccgacca tccgacgatc cgccaacggc
      721 agcaggcctt cggctatacg gtcgaagaac tcaagatggt tatcacgccg atgatcgtga
      781 cgggcgaaga agcgatctcc tcgatgggca ccgatacccc gctggcggtg ttgtccgatc
      841 gcccgcaact cctgttcaag tatttcaagc agctctttgc gcaggtgacg aaccctccca
      901 tcgacccgat ccgcgaacag ctcgtgatgt cgctcgtgac caacatcggt ccgaagccga
      961 acttgatgga cgaatcgccg gagtcttgtc gccgcatcaa ggtgcagcag ccgattctca
     1021 cgaacgccga tttgcaaaag atccgcggca tttccgatcc ccacttcaag agcaagacgc
     1081 tccgtatgtt gttccgcgtc gccgaaggtc cggatgggct tggcgaagcg gtcgacgagc
     1141 tgtgtcagca ggcctcacag gcgatcaagg aaggctataa gttcctcatt ttgagcgatc
     1201 gtggtgttga tgaacagtgg gctccgatcc caagtttgct cggtatctcc gcagtccatc
     1261 atcacctggt tcgtgagtgt acgagaaccg aagtgggctt aatcctcgaa accggcgagc
     1321 ctcgcgatgt gcatcaattc gcctgtttaa tcggttatgg tgccgggaca atcaacccat
     1381 atctgctttt cgagtcgctt gtcgacatgg agcgtgacaa ctatctgcca gaaggcctcg
     1441 acgcgcagac ggcggagggg aaattcatca aggccatcaa caagggcttg ctcaagatct
     1501 tttccaagat ggggatttcg accgtgcagt cctactgtgg cgcgcaaatc ttcgaggcga
     1561 tcggattgaa tcgcgaactc atcgaccgct atttcaccgg gaccccctcg cgcgtggaag
     1621 gggtcgacat ccgcgacatc ggcgaagaaa ccttgcgccg tcaccgcgtg gcttatgagc
     1681 cggcggcgat ccgccaactc gatttcggcg gtgagatcca ctatcgcatc cagggtgagc
     1741 accataactg gaatccggag acgatctaca agctgcagca cgccagccgt tcgaacgacc
     1801 cgaagaccta tgccgaattc gcgcagatcg tgaacgacga gagcaagcgg cggtcgaacc
     1861 tgcgtggcct gctggatttc aagttcgatc ctcagccggt tcccatcgaa gaggtagagc
     1921 cggtcaaaga aatcgtcaaa cgattcaaca ccggcgcgat gtccttcggt tcgatcagca
     1981 aggaagcaca cgaaacgctg gcgatcgcca tgaaccggct cggcggcaag agcaacaccg
     2041 gcgaaggtgg cgaagatccc gagcggttca ttccgcttcc caacggggat tccaagaaca
     2101 gttacatcaa acaagtagcg tcggcccgat ttggagtcac ggcccattac ctcgtgaacg
     2161 cgagagaatt gcagatcaaa atggcgcagg gagccaagcc aggcgaaggc ggccagttac
     2221 cagggcacaa ggtggacgag aacatcgccc gcttccgtta tgccacgccg ggcgtgcaac
     2281 tgatttcgcc gccaccgcac catgacatct attcgatcga ggatttagct caattaatct
     2341 tcgacttgaa gaattccaat ccggatgccg gtgtatccgt gaaactggtg gcggaagtcg
     2401 gtgtcggcac cgtcgcagcc ggcgtcgcca aggcccatgc ggacaaggtg ctcatcagcg
     2461 gcgattcggg cggcacggga gcatcaccgt tggcgtcgat caaatacgcc gggatccctt
     2521 gggagctggg gctggcggaa actcatcaga cgctggtcct caacgatttg cgaggccgca
     2581 tccgtgttga aaccgatggc cagatgaaga ccggccgcga cgtggttatc gccacgctgc
     2641 tcggtgcgga agaatatgga ttcgcgacag cgccgctgat cgtcgaaggc tgcatcatga
     2701 tgcgcaagtg ccatctgaac acctgtccgg tcggaatcgc gacgcaagat cccgaattac
     2761 gaaagaagtt cgatggcaaa ccggagcaca ttgtcaacta cctgttcttc gtggccgaag
     2821 aagcgcggca gttgatggca aaactcggtt tcagaacgat caacgaaatg gtcggccgtg
     2881 tcgacaagct caagatcact aaagccgtcg atcattggaa agccaaggga ctggacctca
     2941 cgccgctgct gacggctcct gatgtcccgg cggatgtacc gcgctattgc gtgcagaagc
     3001 aggatcatgg tctcgccggc attctcgata acaagctcgt cgagctctgc aaggcgtcga
     3061 tcgagcaggg cgagaaggtc aagctcgaac ttccgatcag gaacatcaac cggacgacgg
     3121 gcacggtact ttcgagcaag attgcgaaga agtacggacc ggacgggtta cctgaggata
     3181 cgatttcgat caagtttacc ggctcggctg ggcagtcgtt cggcgcgttc ctggcgaaag
     3241 gcatcacgct gaccctggaa ggggagtcta atgactatat cggtaaggga ttgtccggcg
     3301 gcaagatcat tgtcttcccg cctaagaaca tcctgtatga tccggaagag acgattttga
     3361 tcggcaacac gtcgctctat ggggcaacgc agggggaggc ctatttctac gggatggccg
     3421 gtgagcggtt tgctgtcagg aacagcgggg cgcatgccat cgtcgaaggg acgggtgatc
     3481 acggttgtga gtacatgacc ggtggcgtgg tcgtggtact cggcaggacg ggacgtaact
     3541 ttgcggccgg catgtccggg ggagtggcgt tcgtgctgga cgacgccggg acattccaga
     3601 gccgctgcaa caccgggatg gtcgaactgg aaccggtgac gacgaaggaa gacaaacaac
     3661 tgctccatgg gttgatcacg aagcacttta tgtacacggg tagccgtaag gccaagcaag
     3721 tgctcgatgc gttcgaggcc accttaccga agtttgcgaa ggtgatgccg gtggattaca
     3781 agcgggtctt ggaagaacgg aagcgcaaag cgagcgcggt gcactagctc gcgtataaga
     3841 ggactgagga cagagggccg agtgaagggt agcgaatttc tcagtcctca gtcctgagac
     3901 ctcagtcctg agacctcagt cctggagaga agatgggtga tccaaagggt ttcatgaaat
     3961 atgcccgtga gggcccgaag cgcaagccaa tcgagctacg tgtgctcgat tggaaggaga
     4021 tgtacgaacc gatctctgag gacaagttga aggcccaggg cgcgcgctgc atggactgtg
     4081 gtgtgccatt ttgccaaggc cacaccggtt gccctgtggt caatctaatc ccggagtgga
     4141 acgatctcgt ctaccgcggc cgttggaaag atgcgctgaa agctctgcat acaacaaaca
     4201 actttcccga gtttaccggc cggctctgtc cggcgccctg cgaaggcgcc tgtgtattgg
     4261 ccatcaatga agatccggtt tccatccgtg tcatcgaatg gaacatcgtt gaccgcggct
     4321 tcaacgatgg acttgtgact cccatcctgc cggtggtcaa gacggggaag acggtggcaa
     4381 tcgtcggttc cggtcctgcc
//
LOCUS       DASTWE010000823         1400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_571993, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000823 DASTWE010000000
VERSION     DASTWE010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571993"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1400)
                     /locus_tag="VFT30_08555"
     CDS             complement(<1..>1400)
                     /locus_tag="VFT30_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU5092723.1"
                     /translation="TFDFTWFIPAIVKYRKFFGEVLIASFFLQLFALLTPLFTQVVID
                     KVLVHKGFTTLHVLAIGMIALAIFEAIVGGLRIYVFSHTTNRIDVSLGAQLFRHILAL
                     PMSYFEVRRIGDTVARVRELEQIRQFLTSHSITLVLDVVFTAVFMTVMWVYSPTLTLV
                     VMASLPIYALLSIAITPAIRARLHEKFNRGAENQAFLVEAVSGIQTVKAMAVEPPLVR
                     RWEEQLAGYVRASFRATSVMMIAGQSATCVQKMTTVAVLWLGAYRVIDGDLSIGQLIA
                     FNMLSAQVTGPILRLVNLWQEFQQVGISMQRLGDVLNTQPEPSYNPNRTTLPRVVGQV
                     RFEEVTFRYRPDGPEVTRKMSFSVEPGQVIGIVGRSGSGKSTIAKLLQRLYVPERGRI
                     LVDGVDLAQVDPAWLRRQVGVVLQENFLFNGSVRSNIALTDPGLAMEQVIQAVKMAGA
                     HEFILELAEGYDTLIG"
BASE COUNT          330 a          418 c          367 g          285 t
ORIGIN      
        1 tctccaatca gcgtgtcgta gccctccgcc aattcgagga tgaactcgtg ggccccggcc
       61 attttcaccg cctggatcac ctgctccatc gccaaacccg gatcggtcag cgcgatgttg
      121 cttcggaccg agccgttgaa caggaaattt tcctggagca cgactcccac ttgccggcgt
      181 agccatgcag gatcgacctg tgccaaatcc actccatcca ccaaaatacg cccccgttcc
      241 ggcacataga gacgctgcag caacttggcg atggtgcttt tcccggatcc cgaacgtccg
      301 acaatgccga tgacctgtcc aggctcaacg gaaaaggaca ttttcctggt gacttccggc
      361 ccatccggcc ggtaccgaaa cgtgacttct tcgaatcgta cctgccccac gactcgaggc
      421 aatgtcgttc gattcgggtt atacgatggt tcgggctggg tgttgagcac atcccccaac
      481 cgttgcatcg agatgcctac ttgctgaaat tcctgccaga gattcacaag ccgaagaatc
      541 ggccctgtca cctgtgccga cagcatgttg aaggcgatca actggcctat actcaggtct
      601 ccatcgatca cgcggtaggc acccagccac agcaccgcga cggtggtcat cttttggacg
      661 caggtcgcag attgaccggc tatcatcatc acgctcgtcg cgcggaaact cgcccgcaca
      721 taaccggcca gttgttcttc ccatctccgc accagcgggg gctcgaccgc catggccttg
      781 acggtctgga tcccgctcac cgcttcgact aagaacgcct ggttttcggc gcctctgttg
      841 aacttttcat gcaaacgcgc tcggatcgcc ggagtaatgg ctatcgacaa caacgcatag
      901 atcggaagcg aggccataac gacgagtgtg agcgtgggac tgtataccca catgaccgtc
      961 ataaaaacgg cggtgaacac gacatccagc accagcgtga tggaatggct ggtcaagaat
     1021 tggcggatct gttccaactc acgtacgcgg gcgactgtat cgccgatacg tctgacttca
     1081 aaataggaca tcggaagcgc caggatatgc cggaagagtt gggcgcccag gctgacatcg
     1141 atccgattag tcgtatgcga aaacacgtag atccgaagcc cgcctacaat cgcttcgaaa
     1201 attgccagcg cgatcattcc gatcgccaac acatggagcg tcgtgaatcc cttgtgtacg
     1261 agtactttgt cgatgacgac ttgtgtaaag agcggcgtca acaaggcgaa gagctgaaga
     1321 aagaacgacg cgatcagcac ttcgccgaaa aacttacgat atttgacaat ggccgggatg
     1381 aaccaggtga agtcgaacgt
//
LOCUS       DASTWE010000824         1795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_992115, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000824 DASTWE010000000
VERSION     DASTWE010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992115"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFT30_08560"
     CDS             <1..653
                     /locus_tag="VFT30_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092724.1"
                     /translation="LTELDELKPSSSEPLPKDRGFGLIQAAAERMKRCTSRYHGGHYR
                     KIMRVPARLSVAARSHGLFVNCRGDHDEITFVGKSVKNMRPRALIFFVFTGLLWSFPV
                     AGWSTDSLPAEPDLNTRVDELYDHEARLFIMLYSLSGNGKVDYVTARLVQEYSRSIYG
                     NPIYQTEAQPLFYWWNHIMWNDPEQDGVNGNEKVYQENTDFDISRYKPCLFNGQPC"
     gene            805..>1795
                     /locus_tag="VFT30_08565"
     CDS             805..>1795
                     /locus_tag="VFT30_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5092725.1"
                     /translation="MKQRAFDLAGLMNRRILVMLPLGFASGLPLALTSGTLQAWLTVE
                     GVDLKTIGIFTMVGLPYTLKFLWAPVMDRVIPPWFGRRRGWMVLTQLCVAIGLVLMAA
                     TNPKIHPGWMAAYALLVAFLAASLDIVFDAYRTETLHSHERGLGAAVWVNGYRIALLA
                     SGAGALVLADHVGWQMTYLAMAAVMLAGMCIVLISPGPTFVAEAPKSMKEAVGAPLAE
                     FFGRPHALGFLAVIILYKLGDAFASALQTAFLIGGLGFSATDVGAVKGLGIFATLLGA
                     FFGGLLMTRSGLVRSLLIFGALQAVSNLGFVALAVAGKNSSVLAAAVVIENVTG"
BASE COUNT          325 a          465 c          543 g          462 t
ORIGIN      
        1 ggttgaccga actcgacgag ctcaaaccct cttcgtctga gcccctcccc aaggatcgag
       61 gtttcggctt gattcaggcg gcagccgagc gtatgaagag atgcacgagt cggtaccatg
      121 gggggcatta taggaagatc atgcgtgttc cggcaaggtt gtctgtcgcg gccaggtcgc
      181 acggcttgtt tgttaattgc cggggagacc atgatgagat cacattcgtc gggaagagtg
      241 tgaaaaacat gcgtccgcgt gctctcatct tttttgtttt tacaggattg ctctggagct
      301 ttcctgtggc gggatggagc acggattcgc ttcccgccga gcccgatctc aatactcgtg
      361 tcgatgagct gtatgaccat gaggctcgtc tctttatcat gctgtattct cttagcggaa
      421 atggcaaagt ggactatgtc acggcccgcc tggttcagga gtattctcgc agtatctatg
      481 gtaatcccat ctaccagacc gaggcccaac cgctgttcta ttggtggaac cacatcatgt
      541 ggaacgatcc ggagcaggat ggggtcaacg gcaacgaaaa ggtctatcag gaaaataccg
      601 acttcgatat ctcgcgatat aagccctgtt tgtttaatgg acagccctgc tagtgcctgc
      661 gccggccggt gaggccgctc ccgcatattt tgtgattctg ctgaacagcc ccagagggcg
      721 atggtattct gccttgcggt gaaaaaacgc tccggtgtgg tgtaggtctg actgttcggc
      781 tcacgttcgt atgagagagt ctccgtgaaa caaagagcct tcgatcttgc cgggttgatg
      841 aatcggcgca tcctcgtgat gctgccgttg ggttttgcgt ccggcctccc tctcgcgctc
      901 acgtccggga ccttacaagc ctggcttacg gtggaaggag tcgacctcaa gaccatcggc
      961 attttcacaa tggtcggcct tccctatacg ctcaagttcc tttgggcccc cgtgatggat
     1021 cgtgtgattc ctccctggtt cggccggcgg cgcggctgga tggtcctgac acaactctgt
     1081 gtcgcgatcg ggttggtgct tatggccgcg accaatccga agattcatcc cggatggatg
     1141 gcggcctatg ccctgctcgt cgcctttctt gccgcttctt tggacattgt ttttgatgcc
     1201 tatcgaacgg aaacactcca ttctcacgag cgtggattgg gtgccgccgt gtgggtgaac
     1261 gggtatcgaa tcgcgctttt ggcctccgga gccggcgcgt tggtccttgc cgatcatgtg
     1321 ggatggcaga tgacgtactt ggcgatggcg gccgtcatgc tggcggggat gtgtatcgtt
     1381 ctgatcagcc caggccccac attcgtcgcc gaggcgccaa agagtatgaa ggaagcagtc
     1441 ggcgcaccac tggcggaatt ctttggacgg cctcacgcgt tgggcttctt ggctgtgatt
     1501 attctctata aacttggaga cgcctttgcg tcggcgctcc agacggcctt cctgatcgga
     1561 ggactcggct tttccgctac ggatgtgggc gccgtgaaag ggctagggat ttttgccacg
     1621 ttgctcggtg ccttctttgg aggactgttg atgaccaggt ccggattggt gcgctctcta
     1681 ttgatcttcg gcgctttgca agcggtctca aatctggggt ttgtcgcatt ggctgtagcg
     1741 ggcaagaatt cgagcgtact ggcggcggcg gtcgtgattg aaaatgtgac aggag
//
LOCUS       DASTWE010000825         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1056731, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000825 DASTWE010000000
VERSION     DASTWE010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056731"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFT30_08570"
     CDS             complement(<1..921)
                     /locus_tag="VFT30_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5092726.1"
                     /translation="MGDVLTNWYHETERALGEIATAVQEHRSISLERLESLAGDVVSS
                     MQYNDELLVQALAGPSGSPLITNLINVAVLGTKVGIGLGYYGEELHRLALAGFVHDIG
                     LFAVPKSLIAKAGRLTQEERGFIEQHPELGYQIVEKCGPTYHWLAQLTRQAHERFNGQ
                     GYPNRLKGREISEMAQIVGVVDVFDALVSERPYRRRLLPHEAIKELLIAERRTFPREI
                     LKALVEQFSVYPLGTTVRLTTGEIGTVAKVNSRYPLRPVVRRDDQQECEGSGSSEIDL
                     SRMPLVSIAEILHPPVVGRITFSKAPSPLAI"
BASE COUNT          234 a          297 c          258 g          262 t
ORIGIN      
        1 tattgccagt ggggaaggtg ctttcgaaaa tgtgatacgt ccgaccacgg gaggatgtag
       61 gatctcggca atcgatacta atggcatgcg gctcaaatca atctcagaag aaccactccc
      121 ctcacattcc tgctgatcgt cccttcgcac gaccgggcga aggggatagc gactattcac
      181 tttagccacc gttcctattt ctccggtcgt caatcgcacc gtagtcccaa gcggataaac
      241 cgaaaattgc tcgacgagtg cctttaatat ttctcgagga aaggtccttc gttcagcgat
      301 caaaagttcc ttgattgctt catgcggaag caggcgacgt cgataggggc gctcgcttac
      361 caatgcatcg aatacgtcga cgactcccac gatctgagcc atttcactga tctctcttcc
      421 tttgagtcga ttggggtatc cctgcccgtt gaagcgttcg tgagcctggc gcgttaattg
      481 tgccaaccag tgatacgtcg gtccgcactt ctcgacaatc tgatacccca gctcaggatg
      541 ctgttcgatg aagccgcgct cttcttgggt taaacggccg gccttggcga ttagggattt
      601 cggtacggca aataatccaa tatcatggac aaacccagcc agtgccaacc ggtgtaattc
      661 ttccccatag tacccaagcc cgattcctac ctttgttccg agtacggcca cattgatcag
      721 attggtaatg agtggcgatc cggacggtcc tgctagtgct tggaccagga gctcatcgtt
      781 gtattgcatc gacgaaacga cgtcccccgc aaggctctca agtcgttcca gtgatatgga
      841 acgatgctct tgcacggcgg tcgctatctc gcccaacgca cgttctgtct catgatacca
      901 gttcgtcaag acatcaccca tgtgctgcct cgtcgacgca gcactttttg agtcagacta
      961 agacttcgta ccgcatcgac agagtggcac tcgtacgcct ggttcatttc agcgtcagcc
     1021 cgagcggaac ttcggctatg gagttcattc a
//
LOCUS       DASTWE010000826         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_766219, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000826 DASTWE010000000
VERSION     DASTWE010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766219"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..898)
                     /locus_tag="VFT30_08575"
     CDS             complement(50..898)
                     /locus_tag="VFT30_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092727.1"
                     /translation="MPQNESFGIDSSVALDGLKSLILLLTLVAARSLIVRWIAHNPTL
                     SMESKRRWVVTTRNSVVFAFLIGLVIIWAHELQAFAVSIVALAAALVLATRELILCWS
                     GAALRVGSKVFAVGDRIQIAGHRGVVLDHDAFATRLLEIGPGQSAHLYTGRVTIFPNS
                     LLLTNTLIKENPEQEYGLYTLIVPVKNDDDWQKAEHALLEAAKAECAPFMEEAARQMK
                     LLEQTNLLEAPSPEPRITIQLPESGKIHLVLRFPAPDRGRSRIEQAILRRHLLQMNNQ
                     ESLHRT"
BASE COUNT          219 a          270 c          280 g          232 t
ORIGIN      
        1 gttcacgata aggagtaatc ggagggccat cgctagatgg ccctcttttt taggtgcggt
       61 gcaatgattc ctgattgttc atctgtagga gatgccgacg tagaatggcc tgctcgatac
      121 gggagcgtcc ccgatctggg gccgggaatc ggagcacgag atgtatcttg cctgattccg
      181 gtagctgaat cgtgatgcgc ggttccgggg aaggcgcttc taaaagattg gtctgttcca
      241 atagcttcat ctgccgcgcc gcttcttcca tgaatggcgc acattccgcc ttcgcagcct
      301 ctaagagggc gtgctcggct ttttgccagt cgtcatcgtt tttgaccgga acgatcaacg
      361 tatagagacc gtattcttgc tcaggatttt ccttgatcaa cgtattggta agcagcaagc
      421 tattgggaaa gatggtcacg cgaccggtgt acagatgagc ggattgccct gggccgattt
      481 ccaataacct tgtcgcgaag gcatcgtggt ccaacactac tccgcggtgg cccgcaattt
      541 gaatccgatc accgacggca aagaccttgc tgcccactcg aagcgccgcc ccgctccagc
      601 aaaggatcag ctctcttgtg gccaggacga gagcggctgc cagagcgacg attgaaacgg
      661 caaaggcttg cagttcgtga gcccatatga tcacgagacc gataagaaat gcgaagacga
      721 ccgagttcct ggtcgtgacg acccaacggc gtttcgattc catcgagagc gtcggattgt
      781 gagcgatcca acgcacgatc agcgacctgg cagcgaccaa ggtcagcaac aggatcagcg
      841 acttcaatcc gtccaacgcg accgagctgt cgattccaaa cgactcgttt tgaggcacga
      901 tcgtccttta cttggcggtc agtgtttcac gggtacccgg gacgcattcc tcgcgtttcc
      961 agttcttcaa ggtcttgtct tcagagaacg tcaaggtgta c
//
LOCUS       DASTWE010000827         1863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_862641, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000827 DASTWE010000000
VERSION     DASTWE010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862641"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFT30_08580"
     CDS             <1..264
                     /locus_tag="VFT30_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEU5092728.1"
                     /translation="VITDSVLAQRAMRALIPGVGLALLVASTLHSVATGNLLPATVPT
                     VCVDVNPSVPTKLADRGSFQAVGLVMDAASFLSELARMLGRST"
     gene            261..1058
                     /locus_tag="VFT30_08585"
     CDS             261..1058
                     /locus_tag="VFT30_08585"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEU5092729.1"
                     /translation="MSRLLVCPPDFFGIEYEINPWMRLTNRVNHGRAVRQWHELMRAL
                     EDLGVVLERMTPIPGLPDLVFTANAGVVVGRTAVVSRFRYPERQPEEAHFENWFRGQG
                     YDVLTMEKELYFEGAGDLLGFSEYWFGGYRQRSDIRIFPLLSDRFHREIIPLELVDSR
                     FYHLDTCFCPLSGGELLYFSAAFDSYGQSAIAERVPDKFRLTIPEEEALKFACNAVCV
                     GKHVVLPAECPTTQAWLHLRGYEPHPVQLDEFMKSGGAAKCLTLALD"
     gene            complement(1136..1492)
                     /locus_tag="VFT30_08590"
     CDS             complement(1136..1492)
                     /locus_tag="VFT30_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092730.1"
                     /translation="MQICPTCSRSQPEINRYCIQCGQRLDGRPDNSSDSSANSSGPEQ
                     LNITVLYGMVVVLILAALFPPWETPPTQTPEFLGMYFILSPPAPEAVVSRMLLTIELV
                     TIAIAGMYSAFLFRKK"
     gene            complement(1557..>1863)
                     /locus_tag="VFT30_08595"
     CDS             complement(1557..>1863)
                     /locus_tag="VFT30_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5092731.1"
                     /translation="FNLIFRYHKTRFQPYVGIGPGIFLARVKTTLAGFEGTQSSTRVG
                     LNVKVGGEYFFTKHISGFGEVRYNYTTLNFDANEAGGFGFKAIYNPVIFSFGVNYHF"
BASE COUNT          398 a          504 c          511 g          450 t
ORIGIN      
        1 gtgatcacgg attccgttct ggcacagcgc gcgatgcgtg ccttgatccc cggtgttggg
       61 ctggctctcc tcgtggcttc gacccttcac tccgtggcga cgggcaatct tctaccggca
      121 acggtcccca cagtctgtgt agatgtgaac ccatcggtcc cgaccaagct ggccgaccgg
      181 ggaagctttc aggcggtcgg cctcgtcatg gacgcggcat cgtttctctc ggaacttgcc
      241 aggatgttag ggaggtcgac atgagccgtc tcctcgtctg tcctccggat tttttcggga
      301 ttgagtatga gatcaatccc tggatgcgcc ttaccaatcg cgtaaatcac ggaagggcgg
      361 tgcggcaatg gcatgaattg atgcgcgcgc ttgaggacct aggcgtcgtt ctcgaacgaa
      421 tgacccccat tcctgggttg cccgatctcg tatttacggc caatgccggc gtcgtggttg
      481 gacgaaccgc cgtcgtgagt cgtttccggt atcctgaacg tcagcctgaa gaagctcatt
      541 tcgaaaattg gttccgtgga cagggctatg acgtgctcac catggagaag gaactgtatt
      601 ttgagggagc aggcgatctc ctgggttttt cagaatattg gttcggcgga tatcgacagc
      661 gatcggatat ccgcatcttc ccactcctca gtgaccgttt tcaccgggaa attattccgc
      721 ttgagctcgt cgacagccgt ttctaccatc tggatacctg cttctgtccc ttgagcggtg
      781 gcgagctcct ctatttctca gcagcatttg atagctatgg ccagtcagcg attgccgaac
      841 gtgtgccgga caagttccgc ctcaccattc cggaagagga agccttgaag ttcgcctgca
      901 atgccgtctg cgttgggaaa cacgtcgtac tccctgccga gtgtcccacc actcaggctt
      961 ggctccacct aagagggtat gaaccccacc cggttcagct tgatgaattt atgaaatccg
     1021 gtggagccgc caagtgcctc actcttgcgc ttgactaact ctcgccgttc gtcatccatc
     1081 gcgatgaatc ggtggaatac ggcctctcga cgaatggcgt atgacggctg acgtcctatt
     1141 tcttccgaaa gagaaacgcg ctatacatgc ccgcaatcgc aattgtgacg agttcaatcg
     1201 tcaggagcat gcggctcacc acggcttccg gggctggagg agagagaatg aaatacatgc
     1261 cgagaaactc aggtgtttga gtcggcggag tttcccatgg aggaaatagc gcggctagaa
     1321 tcaggacgac gaccatcccg tagagcacgg tgatgttgag ctgctccggc cctgaactgt
     1381 tggcactgga gtctgaagag ttatctggac gaccatccag ccgctggccg cattggatac
     1441 agtaccggtt gatctccggt tgtgaccggc tacaggtagg acagatttgc atccttgcgc
     1501 attccctgca cgccatctac atacatggaa atgtgccgaa gatgctaggg ggaacactag
     1561 aagtggtaat tgactccgaa cgaaaagatg acgggattat aaatggcttt aaatccaaag
     1621 cctccggcct cgttggcatc gaaattgagc gtcgtatagt tgtacctcac ttctccgaag
     1681 cctgagatgt gctttgtaaa gaagtattct ccaccgacct tgacgttcag gcctaccctg
     1741 gtggagcttt gtgtcccttc aaatcctgcc agcgtagttt tcacgcgcgc caaaaatatt
     1801 cccggaccaa ttccaacata gggctggaac ctcgtcttgt gataacggaa gatgagattg
     1861 aac
//
LOCUS       DASTWE010000828         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1444679, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000828 DASTWE010000000
VERSION     DASTWE010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444679"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFT30_08600"
     CDS             <1..885
                     /locus_tag="VFT30_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092732.1"
                     /translation="KKAAEMIESLREYPAPQAEEIKDEASGKTWAITLYHINEFGSFG
                     ERAILIAQDITKRAELEASLRQSEMMSLLGSLVAGVAHEVRNPLFGISSILDAFETRF
                     SDRTEYLRYTNVLRDEIGRLTILMEELLEYGKPFRGELYLVSMEEMIARSVRACSPAA
                     EVAQINLESKVEESLPKVRIDRRRLSKVFVNLIENAIQHSPQKSSVKIEAQRINDGNH
                     EWVQCVIRDSGAGIPAEDLTKIFEPFFSKRRGGTGLGLAIAQRIMQEHGGKLIAGNNP
                     EGGACMIARFPIPSEGDS"
     gene            885..>1135
                     /locus_tag="VFT30_08605"
     CDS             885..>1135
                     /locus_tag="VFT30_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5092733.1"
                     /translation="MSRNKVLVVDDESGVRFGIRDFLEQHGYEIEEADSCQDAQHLFR
                     SSRPDIVIADYMLPDGTALDLLPRLREIDAGIPLLVLTA"
BASE COUNT          289 a          298 c          318 g          230 t
ORIGIN      
        1 aaaaaagctg ccgagatgat cgagagtctg cgcgaatatc ccgctcctca agctgaggaa
       61 atcaaagacg aagcctccgg taagacgtgg gccatcacgc tctaccacat caacgaattc
      121 ggttcgtttg gtgagcgcgc cattctgatc gcgcaggaca tcaccaagag ggccgagctc
      181 gaagcgtcgc tgcgtcagag cgagatgatg tctctgctcg gctccctagt ggccggtgtt
      241 gcgcatgaag tcagaaaccc gctgttcgga atttcgtcga tactcgatgc gtttgagacg
      301 cgattcagcg accgcacgga atacctgaga tatacaaatg tactgcgtga cgagatcgga
      361 cgtctcacca ttctgatgga agaactgcta gagtacggca agccattcag aggcgagcta
      421 tatctcgtct cgatggaaga gatgatcgca cggtcagtac gcgcctgctc gccggctgcc
      481 gaagtggccc agatcaatct cgaaagcaag gttgaagaat cactgccaaa ggttaggatt
      541 gaccgtcgca ggctttcgaa agtatttgta aatctgattg agaacgcgat tcaacattca
      601 ccgcaaaaaa gctcggtcaa gatcgaagcc caaagaatca acgacggaaa tcacgaatgg
      661 gtccagtgtg tgataaggga ttcaggcgcc gggatacccg cggaggatct gacgaagatc
      721 ttcgagccgt tcttctcaaa acgacgcggc ggcaccggac tgggcctggc cattgcgcaa
      781 cgcatcatgc aggaacacgg aggaaaattg attgctggga ataatcccga gggtggagcc
      841 tgtatgatag ccaggttccc cataccatct gagggagact cttgatgtcc aggaacaagg
      901 tgttggttgt cgacgatgag tcgggagtca gattcggtat ccgtgatttc ctcgaacaac
      961 acggatacga gatcgaagag gcagatagct gccaggacgc gcagcacctg tttcgcagct
     1021 cgcggcccga catcgtcatt gctgactaca tgctcccaga cggcaccgcg ctcgatcttc
     1081 ttccacgact acgagaaatc gacgcgggca ttccccttct ggtcctgact gccca
//
LOCUS       DASTWE010000829         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_184131, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000829 DASTWE010000000
VERSION     DASTWE010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184131"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..308)
                     /locus_tag="VFT30_08610"
     CDS             complement(63..308)
                     /locus_tag="VFT30_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092734.1"
                     /translation="MNTKGIVMLVAGVILFLSVIGIGAWVMTAPLEAGEVETKRCNEY
                     VEELDMLKKYNWFWTPYHRRMAFNDCLTRKLGDPKQP"
     gene            complement(359..448)
                     /locus_tag="VFT30_08615"
     tRNA            complement(359..448)
                     /locus_tag="VFT30_08615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Leu,seq:taa)
     gene            657..1208
                     /locus_tag="VFT30_08620"
     CDS             657..1208
                     /locus_tag="VFT30_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092735.1"
                     /translation="MPIEAVGQEAVLDILPLRPFSFDVSFNDRTFAPTRRIIAVQAGI
                     TALRYGPLELRGIYQYYSHHTPTFITDQHSLYANPRWNNFIDLLDFPNGKPISRILRH
                     VLFGPLEHRAVPYVGAMLGGTLPGRGALSPGYLYGGQIGVRFPVAQGFSVDMGLQYTR
                     FEIDFQGKSDLSRQWLFTIGVRF"
     gene            1260..>1946
                     /locus_tag="VFT30_08625"
     CDS             1260..>1946
                     /locus_tag="VFT30_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5092736.1"
                     /translation="MRVCAIKRFLGSLVLTTLSILLQGCGLVYDAVELIHPLTRDELS
                     SICSRSRLRVGISVEPFRPFVFPAVYTDEGIRVTGLDVELIREIAGAFTSYCGGATPI
                     VPVLHVTRFPDLFIKMNEGQLDLFVSSVSGTVPGARPSGLWFSPPYFHEDGVAAIIQK
                     PEVAERVWEQFRKQNGKLDTLAAVQEGLAGLTVAVQKGRSSHLYAEANLKRIRMVICD
                     SLPASIETKDQ"
BASE COUNT          422 a          554 c          486 g          484 t
ORIGIN      
        1 agcactgaag aatggccaac gcgaggagga tttcaaggag gagcgttctg acggaaagat
       61 ctctatggtt gctttggatc gcctaacttc ctggttaagc agtcgttgaa tgccatgcgc
      121 cggtgatacg gcgtccaaaa ccagttgtac ttcttcaaca tatccaactc ctccacgtat
      181 tcattacaac gcttcgtttc gacctctcca gcttccagtg gggcagtcat cacccaggcc
      241 ccaatcccga tgacactaag aaagagaatt acaccggcaa cgagcatcac aattcctttt
      301 gtattcatgc atccctctat aggttcgttt gcgaccgata catccgattc tcatgtactg
      361 gtgccggaga ccgggatcga accggtacgg gccttttgag cccgagggat tttaagtccc
      421 ttgcgtctgc ctattccgcc actccggcga gagggcggat tttaacatag cgactatggc
      481 gcaccgccac ttttctcacg ccttttctca cccctgacca tgcaaaggtt aacgaggttg
      541 ggctacaatg cgcctgattt acagggtatc agtgaagcga tgcaagatag tgaggtggcc
      601 cgctgcggac acacgtttca cgttatttgg ttgctctttt tctattcgca atggcgatgc
      661 cgattgaagc ggtaggccaa gaggcggttc tcgacatcct ccctctccgc ccgttcagtt
      721 tcgacgtgtc attcaacgat cgcacttttg cgcccactcg cagaatcatc gccgttcaag
      781 ccggaattac agcgcttcgc tacggccccc tcgaattgag aggcatctat caatattaca
      841 gtcaccatac accgaccttc atcaccgacc aacattcttt gtacgcgaac ccccgttgga
      901 ataatttcat cgatttgttg gacttcccca atggcaaacc aatcagccgc atcctacgac
      961 acgtcctttt cgggccgttg gaacaccggg cggtaccgta tgtcggagcg atgctgggag
     1021 gaacactgcc ggggagagga gcactttcac caggctacct gtacggaggc cagatcggcg
     1081 tacgatttcc tgtcgcccaa gggttttccg ttgatatggg actccaatac acacgattcg
     1141 aaatcgattt tcagggcaag tccgacttgt cacgacaatg gctcttcacc atcggcgttc
     1201 ggttttaatc cgatggttct taattctcac gacatatcaa gtcatggagg atatgggtcg
     1261 tgcgcgtttg cgccatcaaa agattccttg gctcgcttgt cctaacgacg ctgagcattc
     1321 ttctacaggg atgcggactt gtgtatgacg ccgttgagct catacacccg ttgacgcgcg
     1381 atgaattgtc atcgatctgc tctcgctcgc ggctacgtgt cggcatttcc gtcgagccgt
     1441 ttcgcccatt tgtttttccc gccgtgtaca ccgacgaagg cattcgcgtc acaggtctgg
     1501 atgtcgaact gatccgagaa atagccggcg cgttcacctc gtattgcggc ggagccaccc
     1561 ccatcgttcc cgttctgcac gtcacccgat ttcccgacct gttcatcaaa atgaacgaag
     1621 gccagttgga tttgttcgtc tcgtcggtca gtggaaccgt tcccggcgca aggccgtcgg
     1681 gcctttggtt ttcgcctccc tatttccacg aggatggtgt cgcagccatc atccaaaagc
     1741 cagaagtagc ggaacgagta tgggaacaat ttcgaaagca gaacggaaag ttggatacgc
     1801 ttgctgccgt ccaggagggc cttgccggat tgactgtcgc cgttcagaag ggacgatcct
     1861 ctcacctgta cgccgaagcc aatctcaagc ggattcgcat ggtcatctgc gattcgcttc
     1921 ccgcctcgat cgaaacgaag gatcag
//
LOCUS       DASTWE010000830         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_119452, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000830 DASTWE010000000
VERSION     DASTWE010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119452"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..>1032)
                     /gene="corA"
                     /locus_tag="VFT30_08630"
     CDS             complement(27..>1032)
                     /gene="corA"
                     /locus_tag="VFT30_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEU5092737.1"
                     /translation="GRSHPTPSEDIAKVKTVACLGGVSLERDVPSEEIRDYIREPSNL
                     VWMDVQDPGPAELSMLSEEFGFHPLALEDLATGQQRPKMDEYKGYTFVVTYGVVPGVD
                     ITTPRTFEVDLFIGRNYLVSLHRGRVPALESALGRWTRGGAMLREGVGFLVYTVMDTI
                     IDAYFPIIDAIEDELAKIETEMFARFRQQDVQRLLELKRTLFALRRVLYPLREIFNLF
                     LRRDQPLFSANTLVYFQDVYDHILRILDVLDIERDMVTSTLDAHQAVISNQINVTMKT
                     LTAVTVLVALAGSVFGAWGMNFEVIPLATAPWGFWGVVSSTISLIVIALVASRWRGWL
                     "
BASE COUNT          237 a          307 c          273 g          215 t
ORIGIN      
        1 gacgatcaca agacacgcct ctcatctcat agccagcctc gccaccggct ggctaccaaa
       61 gctatcacga tgagggagat cgtactgctc acaacacccc agaaccccca tggtgctgtt
      121 gccaggggaa tcacctcgaa attcattccc caggctccga acacggaacc agccagcgca
      181 acgagcacgg tgacggcggt cagcgtcttc attgttacat tgatctgatt ggaaatgact
      241 gcttgatgag catcaagtgt gctggtcacc atatcccgct cgatgtccag tacgtctagg
      301 attctcaaga tatgatcgta gacgtcctgg aagtagacca aggtattggc agagaatagc
      361 ggctggtcgc gccgcagaaa caagttgaag atctcccgca gcggatacag cactcgccgc
      421 agcgcgaaca gcgtccgttt caattcgagc aagcgttgga cgtcttgctg acggaaccgg
      481 gcgaacattt ctgtctcgat ttttgccagc tcatcttcaa tagcgtcgat gatggggaaa
      541 taggcgtcaa tgatcgtgtc catcaccgta tagaccagga agcctactcc ttcacgtaac
      601 atcgctccgc cacgtgtcca gcgtcccaaa gcactctcaa gcgccggaac gcgcccgcgg
      661 tggagggaga ccaggtagtt ccgcccgatg aagaggtcca cctcgaaggt tcgcggcgtc
      721 gtaatgtcaa cgccgggaac cacgccgtac gtgacgacaa acgtataacc tttatattcg
      781 tccattttcg gacgctgctg acctgtcgct aggtcttcca gtgccaaggg atggaagccg
      841 aactcctccg atagcatgga gagttccgcc ggcccgggat cctgcacatc catccaaacg
      901 aggttgctgg gttcccgaat gtagtctctg atctcctcgc tcggaacgtc gcgctcgaga
      961 ctcacgccac ccagacacgc caccgttttg actttcgcga tgtcctctga aggagtgggg
     1021 tgtgagcgtc cg
//
LOCUS       DASTWE010000831         3319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1444709, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000831 DASTWE010000000
VERSION     DASTWE010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444709"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1015..1371)
                     /locus_tag="VFT30_08635"
     CDS             complement(1015..1371)
                     /locus_tag="VFT30_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092738.1"
                     /translation="MPPGLLSKIGIDCMLSKGGDGLSGDGCVYELSSPHPVILNPERL
                     HPGDYVKLRLWFPDETAYSVIELAEVQWVKNEWIKIELLLVSPKDQARLRQFVVSEGN
                     HLPVSTRIGEQIMIRA"
     gene            complement(1878..3158)
                     /locus_tag="VFT30_08640"
     CDS             complement(1878..3158)
                     /locus_tag="VFT30_08640"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5092739.1"
                     /translation="MAGKQVVIIGGGFGGLTVARSLRHADVTIIDRTNHHVFQPLLYQ
                     VATAALSPGDIAWPLRTLFRSQRNVRVMMDDVVSVDRTARMVNLRNNSPIVFDILVVA
                     PGSRHAYFGHDEWESVAPGLKTMTDAVQLREKMLLAFEEAERRRTSTGARSQLTFVIV
                     GGGPTGVELAGALAELGRKAMGPDFPNLRLEDLSIMLVEAGPRILPGFDAELSAKAAA
                     ALTRMGVMIKLRNPVSAVHADGVRVGQEWIPSTNVIWAAGNRASPLLNTLSAPQDSFG
                     RVKVRQDLTIPDDPWIFVIGDAAHCGGHDGKPLLGVAPVAMQQGRYVANLIDHDVPPE
                     QRSPFVYKDRGMLATIGRAQAVAQLGPIHASGLFAWLLWCVVHIFFLISFRNRLRVMS
                     EWVWYYLTFKPGAKLIFERPATARSIPSTDHGRE"
BASE COUNT          809 a          827 c          867 g          816 t
ORIGIN      
        1 caatcgtgag gactggcatc tcggcgctgg tgtcggtcat gaagtctcct taaccgaatg
       61 gataactggg ttctatgtgg agaacggatg ccatgaaatg cactctaacg aagtctgagc
      121 cagttgttgt tggtggttag atattggcgg gataaaccat ctttcaaggg ccagttcatt
      181 cgttcgaaaa actttcgtag gaagttcgat ggttggactg cggttacaga tttatcatgg
      241 actgcgttag attggtcttc attattcaga agggagatcg cataacgaag tccgacatgg
      301 cttggtcgca cgacaccgct gagggtcagc atatgaaggg ctgccccttt ttgtcgtgtg
      361 agcttcacat aagttcgcac ttgccaaaat ggcatgtagt gacctcccat tttcgtgagc
      421 caagccaacc tgccgaagtc agatccacag gatgctattg aagtccgtat ccagacttcg
      481 cggatggccg cattgacggc aacgaacgaa taggctggag tcgtctgacg ttccggaagc
      541 tcgaaggaca agttggtagg tcttccctcc acagaaggga cacgcttttc cgtgaagatt
      601 ttgttggata ggtcctagcc ggttgagttg tttttccatt atccgccccc catccatttc
      661 cattaactat gttgtgatcg gtctgctgtc agaatagggc tacatcggtg cgagtggaaa
      721 taccatgaat gtatgaccat accgagccaa agtagatgat cgatttcgct tatagctggt
      781 gtagagccgt acatggttcg cattaacatg gttgcgccat ggtcctcagt ttggaagaga
      841 caaaattatg ggtgaaggtg gatttcttct ggagggagaa gaaatctctt gttgctgacg
      901 ggtgggcatc cgcaagcaac taccttcacc catgtatatt ccaccaaacg cttttgttga
      961 tgacaaggtc gtagtttttc ctaatctgac ggctgctgaa cctagcaggt gcagtcacgc
     1021 gcgaatcatg atttgttcgc ctatcctggt tgataccggc aagtggtttc cttctgaaac
     1081 cacgaactgt ctgagccgcg cttggtcctt gggtgaaaca agaaggagct cgatcttgat
     1141 ccactcattc ttgacccact gaacctccgc taattcgatc acgctataag cggtttcatc
     1201 agggaaccac agacggagtt tgacataatc gccagggtgt agcctttcag gatttaggat
     1261 gacaggatga ggggatgaga gttcatacac gcagccatca ccactcaatc cgtcgccacc
     1321 ctttgaaagc atacagtcga ttccaatttt tgataggagt cctgggggca tgctctctcc
     1381 tttcatggaa ggtctgaacg atgaggaaac ggtaatacag gaatgggagt taggctacta
     1441 ccctggtagg tggaaagacc taaactaaag aggcattcac catgattctt atatcattga
     1501 gcgaaacact tatcgcatat aagaatcata aaactaacga gttatgacat ttatctaatt
     1561 ctatagatta tgttaatgaa aggatgaaag tcacggagca ggtaatagtt tttctcgttc
     1621 actaggactt cttggcgata aactaaataa cgatcggtgg gattattttc tttagctgca
     1681 tctgagtaga ccttttgata ataactttcc ggcctggctg cccggctgaa tactttgcaa
     1741 ctcaatgcga cgccctgcct aacgccttat gagctgaaca acgacagatt cggactcgct
     1801 gttggggtct atggacgatt tgccgaaccg ccgcacctgc agtaaggcag ttcctaatta
     1861 atacttgggt ctttaactca ctcacggcca tgatctgttg atggtatgct acgagcggta
     1921 gccggccgtt caaaaatcaa cttcgctcct ggcttaaaag ttaggtagta ccagacccat
     1981 tccgacatga ctcggagtcg gtttcggaag ctgatcaaaa agaaaatatg gacgacacac
     2041 cagagcaacc aggcaaagag tcccgaagca tggatggggc cgagctgagc cacggcttgt
     2101 gcccgtccaa tcgtagccag catgccccga tctttgtaaa caaatggtga tcgttgctct
     2161 ggggggacat cgtgatcgat tagattggca acatatcgtc cctgttgcat cgcgacaggc
     2221 gccactccca gtaacggctt cccatcgtgc cccccgcaat gcgctgcgtc accgatgacg
     2281 aagatccacg gatcgtcggg aatcgtcaga tcctgtctaa ctttgacccg accgaaggaa
     2341 tcttgtggag ccgagagagt gttcaggagg ggagacgctc gattgcccgc cgcccaaatg
     2401 acgttcgtcg aaggaatcca ttcctgtcca accctcacac cgtcggcatg aacggcactc
     2461 acagggtttc tgagtttgat catcacgccc atgcgagtaa gtgcagcggc tgcctttgcg
     2521 gaaagttcgg catcaaaccc cggtagaatg cgtggaccgg cctccacgag catgattgaa
     2581 aggtcctcaa ggcgcaagtt tggaaagtca ggtcccatgg cttttctgcc gagctcagcc
     2641 agcgcgccgg ctaattcaac ccccgtcggc cctcctccga cgatgacgaa ggtcagctgg
     2701 ctccgggcgc cggtcgaggt tctccgtcgt tccgcttctt caaacgcgag taacatcttc
     2761 tctcgaagct ggacggcatc ggtcatcgtc ttgagtccgg gcgcaaccga ctcccattcg
     2821 tcgtgtccga aataggcgtg gcgcgagccc ggagcgacaa ccaggatatc gaagacgatc
     2881 ggcgaattgt tccgcagatt gaccatccga gccgttcgat cgaccgatac gacgtcatcc
     2941 atcatgacgc ggacgttacg ctgcgaacga aagagcgtgc gtagaggcca agcaatatca
     3001 cctggcgaca acgcggcggt ggctacttga tagagcaggg gttgaaagac atggtgattc
     3061 gtgcggtcaa tgatggtgac gtccgcatgg cgaagagatc gcgcaaccgt taaacctcca
     3121 aacccaccgc caataatgac aacttgcttg ccagccattc tactgtactc gggaaaagta
     3181 tgatcccatc tgcttgccgc ggcgtgtctt ccgatcacgg tatagcatcc cactgtctgt
     3241 agcagtgtcg gagttcgaac atagtttgtt tcgtgtttcc cgactctatc cgcgtgagta
     3301 agcgatgaac gggaatcgt
//
LOCUS       DASTWE010000832         1205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1024607, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000832 DASTWE010000000
VERSION     DASTWE010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024607"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFT30_08645"
     CDS             <1..183
                     /locus_tag="VFT30_08645"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEU5092740.1"
                     /translation="AYLTVISTSLLLPFELYEVIDRVSLLRVGVLLLNLVIVLYLLIQ
                     LKKHTLHSRVSAARHL"
     gene            267..485
                     /locus_tag="VFT30_08650"
     CDS             267..485
                     /locus_tag="VFT30_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEU5092741.1"
                     /translation="MAVQKLSSKNQIVIPKEARQAMGVKGGDELLVVVKDDLTLVMPK
                     PKRYAKALQGLAKGTYPADYLKRERRSW"
     gene            494..934
                     /locus_tag="VFT30_08655"
     CDS             494..934
                     /locus_tag="VFT30_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU5092742.1"
                     /translation="MSQGLPVQRQRIGLDTNVFIYFLEGHPRYGPWCASLFALIERGQ
                     NAAVTSTVTLLELLVQPYRDKKDELAQKIFALTSTYPKIEWVPLTMNLADRAAELRAH
                     YRLSTPDAIQLATAIGHNAVRFYGNDHGLRRVREIECILVEDLV"
BASE COUNT          276 a          288 c          352 g          289 t
ORIGIN      
        1 gcgtatctga cggtgatctc gacgagtctg ttgttgccgt tcgagctcta tgaagtgatc
       61 gaccgggtgt cgctgcttcg agtcggcgtg ctgctgctca atctcgtcat tgtgctttac
      121 ctgctcatcc aattgaagaa acataccttg cactcacggg tctctgccgc aagacatctc
      181 tgacagattg gatgctgagt ggccgctcgt acattgatgc gctttacatg ctgtggtaaa
      241 gtaaagcctt gtgaaggggg tgttccatgg ctgttcagaa gctcagcagc aagaatcaaa
      301 ttgtcattcc gaaggaagcc cggcaggcga tgggcgtcaa gggtggcgat gaattgctgg
      361 tggttgtgaa ggatgatctg acacttgtca tgccaaagcc taaacggtat gcaaaggctt
      421 tacaggggtt agcgaagggt acctatcccg ccgactacct gaaacgagag cgccggtcgt
      481 ggtaggaact tccttgtctc aaggtcttcc tgtccaacga caacgcatcg gtctcgacac
      541 caacgtcttt atttattttc tcgaaggcca cccacgatat ggaccttggt gtgcctcctt
      601 gtttgcgctg atcgaacgag ggcagaatgc cgcggtcact tcgacagtga cgcttctcga
      661 attattggtt cagccctatc gcgataagaa ggatgaattg gcacaaaaga tttttgctct
      721 gaccagcacc tatcctaaga tcgagtgggt gccgctgacg atgaatctgg ctgatcgagc
      781 ggctgagttg cgtgcccact accgcctcag tacgcctgat gccatacagc tggcgactgc
      841 catcggccat aatgccgtgc gtttctacgg aaatgatcat ggcctgcggc gagtcaggga
      901 gattgagtgc attcttgtgg aggaccttgt ctagtctcag aggtttttgt atgtgtcgcg
      961 agggagaact gcttgctgtt cgtgaagtgg aaatgggaaa acgatgtgat cgtccggtat
     1021 gagggggacg aagtcagcgg tccaccgtgt tacacgcgag caagcgtatc gaaaacggcc
     1081 tagacctgaa tgttcgagga gccaatgcgg caggctgcac aaagacggcg gagccgtcag
     1141 cgcagagcaa aggctgtaga aaagttattg gcgttgcgaa aacggacgcg acaagtcact
     1201 gacca
//
LOCUS       DASTWE010000833         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_345786, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000833 DASTWE010000000
VERSION     DASTWE010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345786"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFT30_08660"
     CDS             <1..966
                     /locus_tag="VFT30_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092743.1"
                     /translation="AQYPLIRYFDERRGLCLTVKNEIAQKATLTEDQVGLPYISSIGR
                     RLLPLDRSLRVEGRLLILKDRETELTMPLNSDLLVKPSPPDEWPVSPVDWRTVFVQGV
                     PRVSPAEAFEAVPLFPEDDTEISELAAQSFVADYLDDLSEQDREVGRLRSQAEHVFIA
                     NGDAVIATCVLFDRPRDYTVKVRYFAHAQRQAQQLWTQCAEVQRWDWLQRIRMIAQDA
                     CEESLSSYGPYDLIYQWLPYDSFDSLTIMRTIVTRLSELLRRGGNAFVVGPVQLGELL
                     TQERSRLLVHWDQSVASLPTFLMHKTILPKARIKPYLTLFRVGRL"
     gene            1137..1313
                     /locus_tag="VFT30_08665"
     CDS             1137..1313
                     /locus_tag="VFT30_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092744.1"
                     /translation="MPSVLDRVIERELRKELKDALIRFEQQLRQGGVTEENVKNRMRG
                     AKQFVAFLYGRYLG"
     gene            complement(1421..>1689)
                     /locus_tag="VFT30_08670"
     CDS             complement(1421..>1689)
                     /locus_tag="VFT30_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092745.1"
                     /translation="HGVLDALTDGGLGVAFFSPFNTSRFFFAARPVAVSPIGISSFFS
                     EDAFHVLTSEVKWIWIPTIAGFFIVRGLQAVRWTQSAVKQSPGD"
BASE COUNT          400 a          404 c          478 g          407 t
ORIGIN      
        1 gcacaatatc ctcttatccg gtatttcgat gagagacgag ggttgtgtct gactgtgaaa
       61 aatgagatcg ctcaaaaggc tacacttact gaagatcagg tgggtcttcc atatatcagt
      121 tcgatcggac gtcgtctgct cccgctggat cgcagcctcc gtgtcgaagg gcgtttgttg
      181 attttgaaag atcgtgaaac agaattgacc atgccgttga attccgatct tctggtgaag
      241 ccgtcgcccc ccgatgaatg gccggtcagt cctgtcgatt ggcgaaccgt attcgtgcaa
      301 ggagtcccga gggtttctcc tgccgaagct ttcgaggccg tgcccttgtt tcccgaagac
      361 gacacggaga tcagcgaact cgctgcgcaa tccttcgttg cggactatct cgacgatctc
      421 agtgaacagg atcgagaggt tggaaggcta cgatcgcaag cagagcatgt gttcattgcc
      481 aatggagacg ccgtcattgc cacttgtgtt ctttttgacc ggcctcgtga ctataccgtg
      541 aaagtccgat actttgccca tgcacagcgt caggctcagc agctctggac tcaatgtgca
      601 gaggtgcaac gatgggattg gctgcaacga attcgaatga tcgcccaaga tgcatgtgaa
      661 gagtccctgt caagctatgg accatatgac ctgatttatc agtggttgcc gtatgactcg
      721 tttgattcgt tgacgatcat gagaacgatc gtcacaagat tgagtgaact gttacggcgg
      781 ggtggtaatg cctttgtcgt tggcccggtt caactagggg agctgctgac gcaggagcgg
      841 tcgcggttgc tggtgcattg ggatcaatcg gttgcatctc tcccgacctt tcttatgcat
      901 aaaacgatct tgccgaaggc aagaatcaaa ccatatctta cgttgtttcg tgtcgggcga
      961 ttgtaggtct gcgtgcaaac tcgggttgtg tcgtcggatc ttctcccatg caggaactgg
     1021 cttgtatctt gaaattccta tagaattccc ggacgtctgc ggctgcctgg aaggcatgtt
     1081 gtacaggcat gggcgtgagc ggttggatcc tagtttatca atcgggggag gaagatatgc
     1141 caagcgtgct ggaccgagtg atcgagagag aattaagaaa agaattgaag gatgcattga
     1201 ttcggtttga gcagcaattg cgacagggtg gtgtcacgga ggaaaacgtc aaaaatagaa
     1261 tgcgtggggc aaagcaattt gtcgccttcc tatacggtcg atatctcgga taacgaccat
     1321 agggatcgcc cgttgagccg gcacagcctg tcaacttctt catggggcac ttttcagccg
     1381 ttaccgagaa tgaaccgaaa cggtgcgcgc tagatgctga tcaatctccc ggggattgct
     1441 tcacagcaga ttgagtccac cgcacagctt gtagcccgcg aacgatgaag aaccccgcaa
     1501 tggtcgggat ccaaatccat ttcacctcgc tggtaagcac gtgaaaggca tcctcactaa
     1561 agaatgagct gatgccgatc ggggacacgg caaccggtcg agccgcgaaa aaaaatcggc
     1621 ttgtattgaa cggtgagaag aaggccacac cgagcccgcc gtcggtcagt gcatcgagca
     1681 caccatgtg
//
LOCUS       DASTWE010000834         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1541488, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000834 DASTWE010000000
VERSION     DASTWE010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541488"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            732..>1184
                     /locus_tag="VFT30_08675"
     CDS             732..>1184
                     /locus_tag="VFT30_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092746.1"
                     /translation="MAHAAVADVGDHQVATIPVGNDTARNHKRTGILRTLSVIPGFAY
                     ILALFVAGALMLPDPRATLIDLGPFKLSWVEIILLLSAVIASAEILKVSEPGVDNTGA
                     VIAMGLVAVIQFGLVIASLYDQKFAIFKTTESLMMLIINGVQTYVAFRI"
BASE COUNT          253 a          332 c          341 g          258 t
ORIGIN      
        1 cctcgagttt cataggctcc gctcgcgcat ccccctggat tataccgagt tcggcgcata
       61 ggcggccttg gaagcgatgt gcacgtaacg gacagcgcaa ccgagatggc cagaggcggt
      121 ctaccggtgg ggctgccagc cagattgcga cgagctgccg tggaaggacg agtacgatgc
      181 caagaagctc aagccaaaaa ctgtgatgcc caagcgacct aaaagtcaga accgacccgc
      241 cgactgatcg gcaacgcgtc gcatccatcc gatgtcattg tgggcacgga tgtcgagagg
      301 gcaagagcag ctctcaccaa ggctgccgtc ttccccgcgg agacagcagc caaggcaatc
      361 gccagtgtcg tgaggatcaa cgacaccctt caactgagca tggaatgctc tcagaggcac
      421 tgatgtagat cagtgcctgc accttggcgt ggaactatcc tgcttttttg cacagcggtt
      481 gcattccagc caggtaggct agtcatcttt tcgagcctgc tgctttctga ggcgctcgat
      541 gtcttcgggt tgctcgtcgt gcgcatcatg gcgttgctgg ggcgcgctga cagtggacgt
      601 ccaactgctg agccatcgct acgaattgag tagtttccga gcctacgtcc gtcattgcac
      661 gatcaccaga ttatggtgaa cggaagacag ttcctccggc tcgaaaacca caaccaggaa
      721 tggccgcaca tatggcacat gcagcagttg cagatgttgg agaccatcaa gttgcgacga
      781 ttccggtcgg caacgacaca gcgcgtaacc acaagcggac gggtatcctc cgaactctgt
      841 cagtgatccc agggttcgcc tacatcctgg cactatttgt tgccggggct ctgatgttgc
      901 ccgatccgag ggcaactttg atcgaccttg gccctttcaa gttgagctgg gtggagatca
      961 tcctgctcct ttccgccgtg atcgcgtctg ctgagatctt aaaggtctcg gagcccggtg
     1021 tggacaacac gggcgcggtg atcgcgatgg gactggtggc ggtgatccag tttggtctcg
     1081 tcatcgcgag cctgtatgat cagaagttcg cgattttcaa aacaactgaa tctctgatga
     1141 tgctgatcat caacggcgtc caaacttacg tcgcgttccg gatc
//
LOCUS       DASTWE010000835         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_797997, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000835 DASTWE010000000
VERSION     DASTWE010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797997"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFT30_08680"
     CDS             <1..464
                     /locus_tag="VFT30_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5092747.1"
                     /translation="VAGGLFALGLVFCNMVILPLVIDLFVSFGLDRDITPQLSVGTYI
                     DFNVKFLLIFGCAFELPLAMSLAAMTGMVSADTFTRYRKHAVLLCLIISAIVTPDATL
                     FTMLLMAVPLMALYEIGILGARLFGRNQDAGGIDLPLDPDLPINTAGTRLR"
     gene            491..1546
                     /locus_tag="VFT30_08685"
     CDS             491..1546
                     /locus_tag="VFT30_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092748.1"
                     /translation="MRTIASVMLLLWGVFFCSAGKESLAASDPLVLPTESSTASGPSE
                     QSAASVQALVMGASGLIYAGSFGHGIFRTMDRGTTWVRVGGGVTDPFILSLASTKDGA
                     VYAGTFRGGVFRSRDEGKSWQSVNAGLKQLEIKTLLAVDHELFAGTGDGVYRLDGSED
                     RWTPVTPGLDDILVHAVARSADGTLFAGTSGKGIFRFSPRTTGWVRMRHGLKDHEGMI
                     ENFIRVLVIDQDQSILAGTFDGGVFRSADSGLTWRPISRALPNDSIRGIVFSDQGVVV
                     ATGNGIFKSVDKGKQWIPVNKGLANLAVQVLIGSGDKGLYAGTSSGVFRSDDGVSWMA
                     VNQGLEAGIAPPPFLFR"
     gene            1566..2078
                     /locus_tag="VFT30_08690"
     CDS             1566..2078
                     /locus_tag="VFT30_08690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5092749.1"
                     /translation="MADGGDRIRATIAVTSKGQPVGEIVLRFFHDVAPGHVSNFVKLS
                     REGFYNGTTFHRVIPGFMIQGGDPNSKNSDRSSHGMGGPGYKVKAEFNSTPHKRGIVS
                     MARANDPDSAGSQFFICIADANFLDWQYTVFGEVVSGMEVADKIVNMKRDGRDNPLER
                     AEMTVTISEG"
     gene            2089..>2474
                     /locus_tag="VFT30_08695"
     CDS             2089..>2474
                     /locus_tag="VFT30_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092750.1"
                     /translation="MKVSCKETNNRTGWIATVLLTMYSVLSVGCAAPHVPSRIIYEDP
                     ANFVRLEEDPEVLPEWPPSHHSHPFTIEPEKLRTVLSGLKVQEHWIALQRWMRGESPL
                     VPAFTDEELALLSVRISEALAEAKDN"
BASE COUNT          516 a          575 c          768 g          615 t
ORIGIN      
        1 tggtggccgg cgggctgttc gcgttagggc tggtcttctg caatatggtg attctgccgc
       61 tggtgatcga cctgttcgtg agtttcggac tcgatcgtga tatcactcca cagctgagcg
      121 tcggcactta cattgatttt aacgtgaaat tcctgcttat cttcggctgt gccttcgaac
      181 tacccttggc catgagtcta gcggctatga ccggaatggt atcagcagac acgttcactc
      241 gttatcgtaa acatgctgtt ttgttgtgtc tcattatttc cgccatcgtc acccctgacg
      301 caacgctctt taccatgctc ttgatggcgg ttcccttgat ggctctgtat gagatcggga
      361 ttctcggcgc ccggcttttc gggcggaatc aggacgcagg ggggatcgat ttgccactcg
      421 atccggattt gccgatcaac accgccggaa caagactacg atgaggcata gggctgtaaa
      481 ggctcaacgc atgcgcacga tcgcttctgt gatgttacta ctatggggtg tttttttctg
      541 ttcggccggt aaagagagtc ttgcggcctc cgatccgctg gtgttgccta cagaatcttc
      601 gacggcatcg ggaccctctg aacaatccgc agcaagcgtg caggcacttg tgatgggtgc
      661 cagtgggctc atctatgccg gatccttcgg acatgggatt tttcgcacca tggaccgtgg
      721 cacgacatgg gttcgagtag gaggtggtgt cactgatcct tttatcttga gtttggcctc
      781 caccaaagac ggagcagtct atgccggcac gtttcgtggc ggagtgtttc gttcgcgcga
      841 tgaggggaaa agctggcaat cggtcaatgc ggggctcaaa cagcttgaaa tcaagacatt
      901 actggcggtg gaccacgagc tttttgccgg caccggagac ggcgtgtatc gcctggatgg
      961 ctccgaggat cgttggacgc ctgtgacgcc ggggttggac gatattctgg tccatgcagt
     1021 cgctcgctct gccgatggca ccttattcgc ggggacttcc ggtaaaggca tttttcggtt
     1081 cagtcctcgc actaccggct gggttcgtat gcggcatggg ttgaaggacc acgagggaat
     1141 gatcgaaaat ttcattcgcg ttttggtgat tgatcaggac cagagtattt tggctggcac
     1201 gttcgacggc ggagtctttc gcagtgccga cagcggcctg acgtggcgac caatcagccg
     1261 ggcgctaccc aatgactcta tcaggggtat cgtattcagt gaccaaggag tggttgtggc
     1321 gactgggaat gggattttta agagcgtgga taagggcaag caatggattc ccgtcaataa
     1381 gggcctggcg aaccttgcgg tgcaagtgtt gatcggatcg ggggacaaag gtctgtatgc
     1441 ggggacgagt tcgggggtgt ttcgcagcga tgatggtgtt tcctggatgg ccgtcaatca
     1501 ggggttagag gcgggaatag cgcccccgcc atttctcttc cgctaaacta gaaaggacga
     1561 atgtaatggc tgatggaggc gataggatac gggcaacgat tgcggtcacg agtaaggggc
     1621 aacccgtggg agaaatcgtg cttagatttt tccatgatgt ggcgcccggc cacgttagca
     1681 attttgtcaa actttcccga gagggatttt ataatgggac gacgtttcac cgcgtcatcc
     1741 ctggtttcat gattcagggg ggagatccca acagtaagaa ttccgatcgt tcatcccacg
     1801 gaatgggtgg gccgggttat aaggtgaagg cggagttcaa cagcacgccg cacaagcgag
     1861 gcattgtttc gatggcccgc gcgaatgatc cggatagcgc tgggtcacag ttctttattt
     1921 gcatcgctga cgctaatttt ctagattggc aatatacggt atttggggag gtggtcagcg
     1981 gaatggaggt tgccgataag atcgtcaata tgaagcgtga tgggcgagac aatccgctgg
     2041 agcgggctga gatgactgtt acaatcagtg agggatgaac gagaaggggt gaaagtgagc
     2101 tgcaaggaaa ccaataatcg gacaggctgg atcgctaccg tactcctcac catgtattcc
     2161 gtactgtcgg ttggttgtgc cgctccccat gtgccatcgc gaattattta tgaggatcca
     2221 gctaattttg tgcggttgga agaagaccct gaagtgcttc cggaatggcc gccgagccac
     2281 catagccatc cattcacgat cgaaccggaa aaattgcgga cggttttgtc gggactgaag
     2341 gtgcaggaac attggattgc gctgcagcgg tggatgcggg gggagtctcc tctggttccg
     2401 gccttcacgg atgaggagtt ggcccttttg tctgtacgca tatcggaagc gctggcggag
     2461 gcgaaagaca atga
//
LOCUS       DASTWE010000836         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_669037, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000836 DASTWE010000000
VERSION     DASTWE010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669037"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFT30_08700"
     CDS             <1..394
                     /locus_tag="VFT30_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5092751.1"
                     /translation="DMNGRIVSLSELRGKVVLLNFWATWCGPCRVEMPAMERLYRTYD
                     RKDFEILAVSTDAQGVAVTRPFQEENKLTFPILHDADFRVGLSYGARTLPMTFMVDRQ
                     GVVRQHIFGARDWEAPEAHQLIEMLMKS"
     gene            404..1150
                     /locus_tag="VFT30_08705"
     CDS             404..1150
                     /locus_tag="VFT30_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5092752.1"
                     /translation="MSESLTQISMIAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDANARSQFKRAIVVNALLFIAGFSVVFIAFGASASFVGQLLMTYQEHIRRIGGLLI
                     VIFGLYLLGILNINFLQMEHRFQFRNRPVGYLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTDSLLDGVFLLTSYSLGLGLPLFLTALGVDRFLAYFKQARAYLWGVSTVSGVLL
                     VIVGVMIYANSLTMITSFLERHGIGWYLGQ"
BASE COUNT          225 a          344 c          347 g          349 t
ORIGIN      
        1 cgacatgaac gggcgaattg tctctctgtc agaattgcgt ggcaaagtgg ttctgctcaa
       61 tttctgggcc acgtggtgcg ggccatgccg ggttgagatg ccggccatgg aacgtctgta
      121 ccggacgtac gatcgaaaag acttcgagat cctcgccgtg tcgaccgatg cgcagggagt
      181 ggcggtcacg aggccgtttc aggaagagaa caaactcact ttcccgattc ttcatgatgc
      241 cgattttcgc gtcggattgt cgtatggggc acggacgctg ccgatgacat tcatggtcga
      301 ccgtcagggc gtggtgcggc agcacatctt cggtgcccgt gactgggaag ctcccgaagc
      361 gcaccagttg atcgaaatgt tgatgaaatc atagagattg ctcgtgtcag aatcgctgac
      421 ccagatatcg atgattgcgg ccttttcggc gggattactg tcgtttgtct caccgtgcgt
      481 gctgccgctt gttccttcct atatttccta catcacaggt ctatcaatcg agcagctgac
      541 cgatgccaat gcgcggtcac agttcaagag agcgatcgtg gtgaatgctc ttttgttcat
      601 tgccggcttt tccgtcgtct tcattgcctt cggggcctcc gccagtttcg tcggtcagct
      661 cctcatgacc tatcaggagc acatccggcg tattgggggt ctgctgatcg tcattttcgg
      721 cctctatctg ctcggcattc tcaacattaa ttttttgcag atggaacacc gcttccaatt
      781 ccggaaccgg ccggtgggat atctcggttc gttcttaatc ggaatcgcgt ttgccgccgg
      841 ctggacgccc tgtgtcgggc ccgtactcgg cacaatttta ctgtatgcca gcaccaccga
      901 ttccttgctc gacggcgtgt tcttgttgac cagttattcg cttggtttgg gcttgccgct
      961 attcttgact gccttgggtg tcgatcggtt tctggcgtat ttcaagcagg cccgcgccta
     1021 cctgtgggga gtatccactg tcagcggtgt actgcttgtc attgtcggcg tgatgattta
     1081 tgccaattca ctcacgatga ttacgagttt tttggagcgg catggcatcg gctggtatct
     1141 gggccagtag ctttcacaga ctcttccccc atttcttctt cttcttcttc gacaggctgg
     1201 tcaaacaggc atgttcacca gttccgaggc gccccgagac gcgcccgtca ccctgaaagg
     1261 caaca
//
LOCUS       DASTWE010000837         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_766382, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000837 DASTWE010000000
VERSION     DASTWE010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766382"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1241)
                     /locus_tag="VFT30_08710"
     CDS             complement(<1..>1241)
                     /locus_tag="VFT30_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5092753.1"
                     /translation="ALALLCGVVIGKGWERTGHAGETYEELKTFSEVLNQVQKHYVDE
                     TKPKDLIQGAIRGMLSTLDPHSAYMTPEMYKEMQVETRGEFGGVGIQIGVKDNRLAVI
                     APIEGTPAYRAGIKAGDFIIKVNDDPTKDLTLMDAVQKMRGPKGSKVNLTIQRDGTTD
                     PLVFTLVRDTIKIESVKSKVIDNLGYVRLTQFQEATGRDLSKAIKQFKDQKVQGTILD
                     LRNNPGGLLTAAVDVSEQFLPSGKLVVYTKSREGKKDEWFAKSKDQLEDLPVIILVNE
                     GSASASEIVAGALQDWGRAVVVGTTSFGKGSVQTILALGDGSGLRLTTAKYYTPKGRS
                     IQSTGITPDIVVKLPTPVLAKASDGKPAEKDTEGKSVKPPTGKRPHHRLGSLPMRPVR
                     RTARFRYRLKREPNSRLSRMS"
BASE COUNT          238 a          374 c          312 g          317 t
ORIGIN      
        1 ctggacatcc tgctcaagcg agagttcggc tcccgcttca gccggtaacg gaaccgtgcc
       61 gttcttcgga ctggcctcat cgggagactt cccagtcggt ggtgaggcct ctttcccgtc
      121 ggtggcttga cggacttccc ctccgtatcc ttttcagccg gctttccgtc agacgccttg
      181 gcaagcaccg gcgtcggcag ttttacgaca atatcaggag taattccagt cgactggatc
      241 gaccgacctt ttggcgtata atacttcgcg gtcgtgagtc gaagccccga accatcaccg
      301 agggccaaga tcgtctgcac cgaccctttt ccgaacgagg tcgtcccaac cacgacggcc
      361 cgtccccaat cttgcagcgc cccggctacg atctctgacg cactcgccga tccctcgttg
      421 acgagaataa tgaccggaag atcttccaat tgatctttgg atttcgcgaa ccattcgtcc
      481 tttttccctt cccggctctt ggtatacacg accagcttcc cactgggaag aaattgctcg
      541 gacacatcga cggccgcagt cagcaatccg ccggggttgt ttcgtaggtc gagaatggtt
      601 ccttggacct tttggtcctt gaactgtttg attgccttgg aaaggtcacg tccggtcgct
      661 tcctgaaatt gagtgagccg aacatagccg agattgtcga tgaccttcga cttcacactt
      721 tcaattttga tagtgtcgcg gacgagggtg aacaccaagg gatccgttgt tccatcccgc
      781 tgaatggtga gattgacctt actgcctttt ggcccgcgca tcttctggac ggcatccatc
      841 aatgtcagat ccttcgtggg gtcgtcgttg actttaatga tgaaatcccc ggctttgatc
      901 ccggctcgat acgcaggcgt gccttcgatc ggcgcaatca cggccagccg attgtccttc
      961 acgccgattt gaatgccgac ccccccgaat tctcccctgg tttcgacctg catttcctta
     1021 tacatttccg gagtcatata cgctgagtga ggatcgagcg tcgacagcat gccccgaatg
     1081 gccccttgaa taagatcctt cggcttcgtc tcatcgacat agtgcttttg aacctgattc
     1141 agaacctcgg agaaggtttt cagttcctcg taggtttcgc cggcgtgacc agttcgctcc
     1201 cagccttttc cgatgacgac tccacagagg agcgcaagtg c
//
LOCUS       DASTWE010000838         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_281068, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000838 DASTWE010000000
VERSION     DASTWE010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281068"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..>1139
                     /locus_tag="VFT30_08715"
     CDS             124..>1139
                     /locus_tag="VFT30_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5092754.1"
                     /translation="MPLRVAIAEAIICQNILDQPPNLPAELIAEPLVRSAAAQQFQEN
                     QHRYRGLLSSLKQYLRSLRHFEGRKSLILISDGFLSDYVRYELQDVTDMALRSGVIFN
                     TVDVRGLFTTNYEASDQVTVGKDQESFIMLSRKPQMRIDDMQQQELPLRQLSSETGGM
                     HIGNTNDLAAGMRKIIDSQSFYYILSYATPSAKSDGRYHKIKLEVTRPGLNVIYRKGY
                     YAPKEQISFERRKKDDIIEALRAPGNLNEIPIKLSYNYFQMDDSRYQLALMTQVNIRG
                     MKFVEEDSRHKNLISVIVVALDEHDQYIDGLEKTIDLNLTGPSYDALLNHGFTSKMDV
                     KVPPG"
BASE COUNT          284 a          320 c          297 g          238 t
ORIGIN      
        1 ttgttgatgt cgctggcggg agacctgcac aagaaggtgc aaagaggagg cgccctgaag
       61 tccgcctgcc ccgaacttac agatatccaa gcgcagcaga tccagaacaa ccattcggat
      121 ccgatgcctc ttcgggttgc cattgccgaa gccatcatct gccaaaatat cctcgaccaa
      181 ccaccgaatc ttcctgcgga actgatcgcg gagcctctcg tcaggtcggc cgcagcccaa
      241 cagttccagg aaaaccagca tcgctaccga ggcctgctca gcagtctgaa gcaatacctt
      301 cgttcgctgc ggcattttga aggcaggaag tctctgatcc tgatctccga cggatttctt
      361 tcggactatg tgcgttacga acttcaggac gtgacggaca tggcgctgcg ttcgggtgtg
      421 atctttaaca ccgttgacgt tcggggcctc ttcaccacca actatgaggc aagcgatcaa
      481 gtgacggtgg ggaaagatca agagtccttc ataatgctat cgcgaaagcc ccagatgcgg
      541 attgatgata tgcaacagca ggagctcccc ctaaggcagc tttccagcga gaccggtggc
      601 atgcatattg gtaacaccaa cgacctcgcg gctggcatgc gtaagatcat cgacagtcaa
      661 tcgttctact acatcctgtc ttatgcgact ccgagcgcca aatccgatgg gcgctaccac
      721 aagatcaagc tcgaagtcac ccgtccgggg ctgaacgtga tttaccgcaa aggctattac
      781 gctcccaaag agcagatctc cttcgagcgg cgcaagaagg acgacatcat cgaagcgctg
      841 cgcgctccgg gcaatctgaa cgagattccc atcaaacttt cgtacaatta ttttcagatg
      901 gacgattccc ggtaccagct tgctctgatg acccaggtga atattcgcgg catgaaattt
      961 gtggaagagg actcacgaca caagaacctg atcagtgtga tcgtcgtggc cctcgatgag
     1021 catgaccagt acatcgacgg tttggaaaaa acgatcgacc tgaatctgac gggtcccagt
     1081 tacgacgcgc tgctgaacca cggcttcacg tcgaagatgg acgtgaaggt acctccggg
//
LOCUS       DASTWE010000839         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_442796, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000839 DASTWE010000000
VERSION     DASTWE010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442796"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFT30_08720"
     CDS             complement(<1..194)
                     /locus_tag="VFT30_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092755.1"
                     /translation="MLHSVKDLDFDSEVVSHLLVSVLAEAIRFADHWRFLHILLISST
                     VIRFIAHLGKLLLCIGRFLPT"
     gene            complement(616..>1404)
                     /locus_tag="VFT30_08725"
     CDS             complement(616..>1404)
                     /locus_tag="VFT30_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092756.1"
                     /translation="HEAIRANRFAEAAVMYRWLAIGATEAGLCDRYAAVARQLQVVHH
                     IRTLCHRDVSFPLRGVEIEDYFLALAEQPVNEIQIAFQEYVDAFYIQPVGMTNLCCAE
                     PGDGEPQEEWCTQIPVSWDDIQRGELNYDGRRVLDSVSFALLGQKCLKAAGFSTGQFA
                     VASKRSSSPENPVLSSVMFTTKRALIETRRGAAGVRTEVVVVANSGLQWAVAREFVEI
                     EAAEELLLWSAFQTALGISMDQSGRMLQERIIRHQELAQVVAAS"
BASE COUNT          340 a          395 c          356 g          313 t
ORIGIN      
        1 gtgggcagga atctgccgat gcaaagcaac aatttcccaa ggtgagcgat aaacctaatg
       61 acggtagacg aaattagaag gatatggagg aaacgccaat gatccgcaaa ccttattgct
      121 tcagccagaa cagagacaag aaggtggctt actacttcgg aatcgaagtc caaatccttg
      181 accgaatgga gcattcttct ctaatccatt acggcgacct taaagtcgtt gttgacaccc
      241 aagatctggt cttcaagcag gtcgccaggg cagctgccta gtcgatggcg atgctcaact
      301 taagggccga tcctcaggtt tgagcgtcct ccagttcgga acggccccac ctctcgtcac
      361 attttcggaa cttccatcat tctcgccaga cactttccca agctcacttc caaataaaca
      421 cttctaatcg acaggtgggg aggccgaact caaggtcggc gactccctta cgggagacaa
      481 ctgccggcta cgacttcaat cgcttttctg ccggaccggc tgcaaggtga ccgagtgcgt
      541 acacagctca attagagcga gacattttgt ccaactgttc ctcatctgtc ttatcgctgc
      601 aggtcggttc taaatctagg acgcagctac aacttgggcg agttcctggt ggcggattat
      661 tctttcttgg agcattctgc cagattggtc catggaaatg ccgagagcgg tttgaaaagc
      721 ggaccagaga agaagttcct cagcggcttc gatttcgacg aactcgcgcg cgacggccca
      781 ttgcaggccg ctgttcgcca cgaccacgac ttctgttcgg acgcctgctg ccccccggcg
      841 agtctcgatt aacgcccgtt tcgtggtgaa catgacgctc gaaagcacgg gattctctgg
      901 ggaacttgat cgtttcgacg caacggcaaa ttgaccggtg gagaaacctg ctgccttcaa
      961 gcacttttgc ccgagtaatg caaaactcac cgagtcgagt acccgccgac cgtcgtagtt
     1021 gagttcaccg cgctgaatgt cgtcccagct cactggaatc tgcgtgcacc attcttcctg
     1081 cggctcacca tccccgggtt cagcacagca aagattggtc ataccgacgg gctgaatgta
     1141 gaaggcatca acatattctt ggaaagcaat ctgaatctcg tttactggct gctctgccag
     1201 agccaggaag tagtcctcga tctcaacacc cctcaaagga aagctcacgt ctcgatggca
     1261 gagcgttcga atgtgatgaa cgacctgaag ttggcgagcc acagcggcat agcggtcgca
     1321 caggccagct tcggtagctc caatggctaa ccaacggtac atcacagcgg cctcggcaaa
     1381 ccgattcgct cgaatcgctt catg
//
LOCUS       DASTWE010000840         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_249124, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000840 DASTWE010000000
VERSION     DASTWE010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249124"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFT30_08730"
     CDS             <1..216
                     /locus_tag="VFT30_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092757.1"
                     /translation="RSFGYHELTFDAFETALKKTKRLQGNDQAEKRLQEIRGHFADPL
                     AKKPEGGTLGAELMDRFRRYLKGEGTL"
     gene            complement(293..613)
                     /locus_tag="VFT30_08735"
     CDS             complement(293..613)
                     /locus_tag="VFT30_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092758.1"
                     /translation="MSKIVNVDITMYGIAEVLMWCLDRNKGRVPGVDTPGFKKMQELL
                     AQKPQSADYFTLDQFWKKKVTLPLTEDEVATIDRCLYDIPNFDNEPLPQIRHKFWPQQ
                     IEAH"
     gene            786..>1678
                     /locus_tag="VFT30_08740"
     CDS             786..>1678
                     /locus_tag="VFT30_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU5092759.1"
                     /translation="MNILIERNSHAARLLFSLLWLSLLSHGLWACSKSEPYNPPDPFY
                     YFASYKVGKNPTTITTEDLNHDSFTDLVTTNIASNTLSILLGNGDGTFRDQVQLHVCQ
                     EPRSLVMNNFNQDQHADVALACSGGDEVMILLGHGDGKFEEGPRYPVHRAPIALAADD
                     INGDHHTDFVVALRNDKVKVFLGNGTGEFRHGAQYEHGDTPTSVALSDLNADGKLDLV
                     VTNGGPMSNAVSIWLGNGDGSFRDPKDYPTGHRPLGVSFADFNNDHHRDLLVINGEQD
                     SFTTFLGNGNATFRPGKNSGA"
BASE COUNT          407 a          443 c          419 g          409 t
ORIGIN      
        1 cgctcattcg gctatcatga gctgacattc gatgcctttg agaccgccct caagaaaacc
       61 aagcgcctac agggtaatga tcaggcggag aagcgcctcc aggaaatccg aggacacttt
      121 gccgatccat tggcgaagaa gcctgaaggc gggaccctgg gagccgagct catggatcgt
      181 ttccgcaggt atctcaaagg cgaaggaaca ctctagcggt ccgaaactac tcacccgtca
      241 attccaatgc gttctcccga gaatacctag ccgaccggat ctaagctttg cttcagtggg
      301 cttcaatctg ttggggccaa aacttatgcc ggatttgggg aagcggctca ttgtcaaagt
      361 taggaatatc gtatagacaa cgatcgatcg tcgcgacttc gtcttcggtg aggggcaacg
      421 tcactttttt cttccagaac tgatcaagtg taaaatagtc cgccgattga ggcttttgcg
      481 ccaacaattc ctgcattttt ttgaagcccg gcgtatctac acccgggaca cgtcccttgt
      541 tccgatcaag gcaccacatc agaacttcgg ctattccgta catcgtgata tcaacattca
      601 caattttgct catgacgttc ctcctaaaag caacgctgca tcataacgta agcggaagtc
      661 gaaattcaaa ggctgatgaa gtccagccgc agattgcgag ggagaagact gcctctgtat
      721 actgggtctc ctgattccac ctcataggct cattggtcca gcagaattgg gtgtccggag
      781 actgtgtgaa cattcttatc gagcggaact cacacgcggc caggctgctt ttctcactgc
      841 tgtggctgtc tcttctcagt cacggcctgt gggcctgttc caagagcgag ccgtataacc
      901 cacctgatcc tttttattat tttgccagct acaaggtcgg caagaatcct accactatta
      961 ccaccgaaga tcttaatcat gactcattca ccgatctcgt caccaccaat attgcgagca
     1021 ataccctctc gattctgttg ggaaatggtg acggtacatt cagagatcag gttcagctgc
     1081 atgtatgtca ggaaccccgt tccctcgtga tgaacaactt caatcaagac caacacgccg
     1141 atgtggcctt ggcatgctct ggtggcgatg aggtcatgat cttgcttgga catggggatg
     1201 gaaagtttga agagggacca cgttatccgg tgcatcgtgc accgattgca cttgccgccg
     1261 acgatatcaa cggcgatcat catacggatt ttgtcgtcgc tctgcggaac gacaaggtca
     1321 aagtctttct agggaacggg accggtgaat ttcgacatgg agcccagtac gaacacggag
     1381 acactcccac atccgttgcg ctgtccgatc tcaacgctga tgggaaattg gatttggtcg
     1441 tgacgaatgg aggacctatg tcgaatgccg tctccatttg gctcgggaac ggtgacggtt
     1501 cctttcgtga tcccaaagac tatccgacgg gtcaccgacc tcttggcgtg agttttgccg
     1561 acttcaataa cgatcatcac cgggatctac tggttatcaa cggagagcag gatagtttca
     1621 cgacatttct tggcaacggc aacgcaacat tccgacccgg aaagaattcc ggggctga
//
LOCUS       DASTWE010000841         1290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1347743, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000841 DASTWE010000000
VERSION     DASTWE010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347743"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VFT30_08745"
     CDS             <1..154
                     /locus_tag="VFT30_08745"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEU5092760.1"
                     /translation="GTHPATLKDRVASPGGTTIAGLHQLEQGKFRATLMGAVEAATKR
                     SQELAR"
     gene            154..450
                     /locus_tag="VFT30_08750"
     CDS             154..450
                     /locus_tag="VFT30_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5092761.1"
                     /translation="MFVVENFFTALASIVYYLLELYMYVVIARAVISWVNPDPWNPIV
                     QFLEKVTEPALAPIRRMIGWRLGVDVSPIILILLLMFLQRFIVPSLHQMATAIG"
     gene            472..984
                     /locus_tag="VFT30_08755"
     CDS             472..984
                     /locus_tag="VFT30_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU5092762.1"
                     /translation="MKITPLDIQQMVFKTGFRGYDKEEVNRFLEEIAETVEELNRENA
                     VQREKITFLEQQLIELKRTEVTLSNTLVSAQSLAEDVKRSAQRESELVIKEAELKAGE
                     MIRQARVELSNTQRDLSVLQKQRLLMVERLRATLRTFERMLEVEEQEAFQDGASSAEH
                     KLEGESSPAL"
BASE COUNT          272 a          336 c          374 g          308 t
ORIGIN      
        1 cggcacccat cccgcaacac tgaaggaccg tgtcgcctcg cccggcggca cgacgatcgc
       61 cgggctccat cagcttgaac agggcaagtt ccgtgctaca ctgatggggg ccgtcgaagc
      121 cgcgacaaaa cgttcccagg agctcgcgcg ctgatgtttg tcgtcgaaaa tttttttacg
      181 gcattggctt caatcgtcta ttatctcttg gagttgtata tgtacgtcgt catcgcccgt
      241 gcggtgattt cctgggtcaa ccctgatccg tggaatccga tcgtgcagtt tcttgaaaaa
      301 gtgactgaac cagcgttggc gcccatccga aggatgatcg ggtggaggct tggcgtagat
      361 gtgtctccga ttatcctgat ccttcttctc atgtttttac agagatttat tgtgccttct
      421 cttcatcaaa tggcaacggc gattggataa gcatcctaga ggaggacagt catgaaaatc
      481 acaccgctgg acattcagca aatggtgttc aagaccggtt tccgcggcta cgacaaggaa
      541 gaagttaatc gctttctgga agaaattgcc gagacggtcg aagagctgaa ccgtgagaat
      601 gcggtgcaga gagagaaaat cacgtttttg gagcagcagt tgatcgaatt aaagcggacc
      661 gaggtgaccc tgtcgaacac gcttgtgtct gcgcagtcgc tcgcagagga tgtcaaacgc
      721 agcgcgcaac gggagtccga gctcgtcatc aaagaagctg agctcaaggc cggcgagatg
      781 atacgtcagg ctcgggtcga gctgtccaac actcagcgtg atctctctgt tctgcaaaag
      841 cagcggctct taatggtcga acgcttgcgt gcgacgttgc gcaccttcga gcgaatgctg
      901 gaagtggagg aacaagaagc cttccaagac ggtgcctcga gtgccgagca taagctcgag
      961 ggagaatcga gccctgcatt gtgagaggat ggtaaagcga tccttcctct gcgcccttcc
     1021 ttcttcacct gttttcggac ctatccccgt gcgaatgcct agcgcattgt ttttgagcct
     1081 ccggtccacg tggagcgatg cacggctctt tcaagtcgct gcttccatcg cctgtggccg
     1141 ttgtctatga aaggccttcg tgtcattcag gccgcaatgg atcaggcggt gcgggacggc
     1201 gtattccccg gcggggtgtt ggccgtgcgg ttggccggtg agctcaaatg tcttgtcgtg
     1261 tccggttgtc tctcgtcgga tccaaccgat
//
LOCUS       DASTWE010000842         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1282805, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000842 DASTWE010000000
VERSION     DASTWE010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282805"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1180
                     /locus_tag="VFT30_08760"
     CDS             164..1180
                     /locus_tag="VFT30_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEU5092763.1"
                     /translation="MPHMTAVQLSSPGAAFEVVKREIPTPGPNQVRIKVQACGICHSD
                     LFVKEGYWPGLLYPRVTGHEVAGIVDEVGTGVTTWKRGQRVGVGWHGGHCGQCDPCRR
                     GDFMGCQNFRVTGFHDDGGYAQYMIARSEALAAIPDSISPAEAAPILCAGVTTFNSLR
                     HSGAVAGGLVAVQGLGGLGHLGIQFASKMGFRTVAIGRGKDKESLALKLGAVRYLDTD
                     AVDVTKELASLGGASVILATAPDSKAMSALIDGLGVGGRLLVVGASADPLSVTPVQLI
                     MSRRSITGWPSGTARDSEDTLNFCALTGIRPMVETLPLEQAATGYERMLSGKARFRMV
                     LTMN"
BASE COUNT          285 a          357 c          395 g          278 t
ORIGIN      
        1 cagtctaacg tcacgctgct aacaacactt ccaaccctca tacgtctagc taccacgcag
       61 aaccttctca atgcctatat gtttgaagcc tatgccgagt ggattcatga tatttgacaa
      121 gcccgtaaac ggtgtttgat tccaaggctg gaaggagacc gacatgccac acatgaccgc
      181 tgttcaactt tcctctccgg gcgcagcatt cgaagtcgtc aagcgagaga tcccgacacc
      241 cggaccgaac caagtccgca ttaaagtgca agcctgcggg atttgtcata gtgacctgtt
      301 tgtgaaggag ggctattggc ccggcttgct atacccacgg gtcacaggcc acgaagtagc
      361 cggcattgtc gacgaggttg gaaccggcgt gacgacatgg aaacgaggtc agcgagtggg
      421 tgtgggctgg catggtggac attgtggtca atgcgatcct tgtcggcgtg gcgacttcat
      481 gggatgccag aatttccgtg tcacaggatt tcatgatgac ggaggatatg ctcagtatat
      541 gatcgctcgg agtgaagccc tggccgccat tccggattcg atctctcctg ccgaggcggc
      601 gccgatcctg tgcgcgggcg tgacgacgtt caatagcttg cgacacagtg gggcggtcgc
      661 aggaggtctc gtggccgtgc aaggcctggg cggccttggc cacctgggaa tccagtttgc
      721 gagcaagatg ggatttcgca ccgtcgcaat tggtcgaggc aaagacaagg aatccctcgc
      781 gctgaagctc ggagccgtcc gatatctgga cacagatgcc gtcgacgtca caaaagaact
      841 cgcaagcctt gggggcgctt cggtcattct tgcgacggct ccggacagca aggccatgtc
      901 cgcgctcatc gacgggcttg gggttggagg gcgactgctt gtggtcgggg cctcggccga
      961 tcccctcagc gtgacgcccg ttcagctgat tatgagccgc cggtccatta cagggtggcc
     1021 atcagggacc gcccgagatt cagaagacac actgaacttc tgcgctctca cggggattcg
     1081 gcctatggtc gaaacgctcc cgctcgaaca agccgcgacc ggctatgagc gtatgttgag
     1141 tggaaaagct cgattccgca tggtgctcac gatgaactaa tcatagtcat gaggctttca
     1201 atggcgacgg aatccacaac cgtggccgaa cgcgagactg aaactcgcaa caagcagtct
     1261 gttcatgccg cgtttgatgc ttggcggaat ggactggcag gcaatgtgtt taatc
//
LOCUS       DASTWE010000843         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1541583, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000843 DASTWE010000000
VERSION     DASTWE010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541583"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFT30_08765"
     CDS             <1..1415
                     /locus_tag="VFT30_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5092764.1"
                     /translation="PTDWNHAPVVQDNSNQADLKEWWRGFHDPLLNELISQALAANHD
                     LRIATARVREANAIITVAESALYPSVDFFSSGGREKRIDRIIAVPGTEGVKLITPTAN
                     VITGGLAARWEIDIFGARHLEAEATGAQAAGTEEERHGVQVGLLAQVATNYLELRGAQ
                     ERTAILRENIDIQRERLRTLQAFYRAGLTKETEVSRQEALLHSTESALPGLNAAEVTL
                     IHRLSVLLGESPAKLEHRLVAATVHPSDLPGIPNMLPSSLLLQRPDLRLAQTEVTAAA
                     ASLGAARADLFPKVVLSASGGIGALAIGGFPTLVESVYALGAGLTAPIFNAGRIRAHI
                     AAADARLDQVAAKYEKTFLLALEDVENAFVAHTSSKERREQLLKAETAAEKTYRSSEA
                     LYQRGASDYLSVLDAQRTKLSINDERVKAETAVRVSLISLSRAFGGGWAIESPVSQDN
                     DEINSGKNSGPAGADVLLKN"
     gene            complement(1514..1714)
                     /locus_tag="VFT30_08770"
     CDS             complement(1514..1714)
                     /locus_tag="VFT30_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092765.1"
                     /translation="MRQHQSDDLAECQREVPVFATTFSRPFKDQSREWRNGTSLGLTD
                     LEAILTVACEAKEWISPHALKR"
BASE COUNT          521 a          562 c          620 g          474 t
ORIGIN      
        1 ctccgacgga ttggaatcat gcccccgttg ttcaagacaa ttccaatcag gctgatctca
       61 aagaatggtg gcgaggattt cacgatccac ttctgaacga gttgatcagc caagcattag
      121 ccgcgaacca cgatcttaga atcgcaacgg ctcgcgttcg cgaggccaac gcgataatca
      181 ctgtcgcgga gtcagcactc taccccagtg ttgatttctt ttcctcgggc ggtcgggaaa
      241 agcggattga caggatcatt gcagtaccag ggaccgaggg ggtaaagctg atcacaccca
      301 ccgcgaatgt catcaccggt ggtcttgctg cacggtggga gatagatatt tttggtgcca
      361 ggcatcttga agctgaggca acgggtgctc aggccgctgg aaccgaagag gaacggcatg
      421 gagtacaggt gggattgcta gctcaggtgg cgacgaacta tctggaacta cgcggcgcac
      481 aggaacgaac tgcgatcctg cgcgaaaaca tcgacatcca gcgggaaagg ctcagaactc
      541 tgcaggcctt ttatcgtgcg ggtttaacga aagaaaccga agtttccagg caggaagcgt
      601 tactgcacag caccgagagt gcccttccgg gattgaacgc agcagaggtc actctgatcc
      661 atcgccttag tgtgttgctg ggcgaatctc cggcgaaact tgaacaccgg cttgttgctg
      721 caaccgtcca tccttccgac ctgccgggta tcccaaacat gttgccttca agcctactcc
      781 tacaacggcc tgatctgcgc ctcgcgcaaa ccgaagtcac tgcggcagcg gccagccttg
      841 gtgcagcgcg ggcagatttg ttcccaaagg tggtgctatc ggcgagcggt gggatcggcg
      901 cactggccat cggtggattt ccgactctgg tggagagtgt gtatgcgctg ggggcgggcc
      961 tcacggctcc gatattcaat gcgggacgca ttcgggctca catcgctgcc gccgacgcgc
     1021 ggctggatca agtcgcggcg aagtacgaaa aaaccttcct ccttgccttg gaagacgtgg
     1081 aaaatgcctt tgtcgcacat acctcgtcga aggagcgccg cgaacaacta ttgaaggccg
     1141 aaacagcagc agagaaaacg tatcgatcct ccgaagcctt atatcagagg ggagcgagtg
     1201 attatttatc cgtgctggat gctcaacgca ccaaactttc aatcaacgac gagcgtgtca
     1261 aagctgaaac cgcagttcgt gtctccttga tctcgctttc cagggcattt ggcggcggtt
     1321 gggccatcga gagcccagtg agccaagaca atgatgaaat caatagcgga aaaaactcag
     1381 ggcccgccgg tgctgacgtc cttctgaaaa actagaccag ctatactgga attttgtcac
     1441 cttgaaaaga atgagaagag tagatgggac gaagggaagc cccggatggc cggaggcttc
     1501 ccagtgaaga agctcagcgc ttcaaggcgt gaggagagat ccactccttg gcctcacacg
     1561 cgactgtgag aatggcctca aggtcagtga gaccgagcga ggtcccattg cgccattccc
     1621 tagactgatc cttgaacggc cgagagaagg tcgttgcgaa gacgggcact tctcgctgac
     1681 attctgccag atcgtcgctt tgatgttgcc gcatcgcagc tggttcgcgg gtttgttgtt
     1741 ggtagccatc tttttcgctc ctttgttgaa atgaagttgg ctctcacagg aagaggagag
     1801 ccaatgcaca tttcacggag gaaacgcgag acaggggtcg tcgccggcgt gccggagagg
     1861 gtgaggtgaa atggttgatt tattctctca accattgggt atggacctcc tcaaaacttc
     1921 cttagcttga ttcaagcatg aatccaggcg atgaatttct catacacccg tatcacggat
     1981 tttcaaatcg gaaatccggg ataagggagc caggtcacaa cagcagcgac ttcacacaat
     2041 aagctgttca aggactgttt tggcatcaat caagtcgcgg ttgtcgacgc cttcggtgaa
     2101 tctggagtac acggaattta agatgcgctt ggcatcatcc gatttattga gtttcttcca
     2161 aagcttcgct aggctcg
//
LOCUS       DASTWE010000844         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_669157, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000844 DASTWE010000000
VERSION     DASTWE010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669157"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFT30_08775"
     CDS             complement(<1..360)
                     /locus_tag="VFT30_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092766.1"
                     /translation="MDGLGRHEHRNLENTHVLDTTKEPAPEVIDAIETDDEVESSFFS
                     TWTLPIVLFLLTVFTTLWAGAYQVYNGPARGPLNFLLTSPETLWRGIPFAGALLFILT
                     THELGHYLLSKIHRVPAS"
     gene            complement(372..1214)
                     /locus_tag="VFT30_08780"
     CDS             complement(372..1214)
                     /locus_tag="VFT30_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5092767.1"
                     /translation="MPLEDDFSDILKKARTGQGLSVGDIARMTGLPGGDITALERGDQ
                     PRDRAEVRALAKALGLRAEPLEQIAIDKWEPVAQRMPSWVETVHGSINGYGVQGYVVH
                     NEGEALLVDTAYNAPAMLDVLQRHRLRLIGICLTHGHADHAYGIEQILGYREVPVYLG
                     PEDMNLLSWRPRTDLLVAPIDGLSISVGHRTVHCLTTPGHTPGGICYRLDDAQLPVCF
                     VGDTLFAGSIGRSNPKELYSTHINSVRDRLLTLSPDYRLLPGHGPATTVEEELDHNPF
                     ATIQ"
     gene            complement(1302..>2514)
                     /locus_tag="VFT30_08785"
     CDS             complement(1302..>2514)
                     /locus_tag="VFT30_08785"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5092768.1"
                     /translation="GGWLDPIDKIGLAAMTGAVMRTGGGGGLSAEQVDTELEQFAADV
                     SIGIGRQSGSASLDVLSKDVNRGLEIFAGLIRTPAFDPARVELARLQAIEAIRRRQDN
                     PGSVVGREFAKMLYGADHPSARESSLDSVKRITRDDLVTFHRHTIHPNGMILGVTGDF
                     HKAEMVTSLRKVFGDWKKGTVPELKISDVPEAELARPVVRFVGKDTSQTHLRVGHLSI
                     KENDPDYVALAIANDILGGSSFRSRLFNDVRSKRGLAYSVGSRLNTGMHDQGVWLMRA
                     ETKLVSTQEVIERFVANIERIRAEPVTEAELAEAKEAYVNSFVFSFSSPSAIVSRLVE
                     FEYDGLPKDFLQQLRAQVVRLTKEDVLAAAKKHLHPDRLKIVAVGSGEALPKALATFG
                     DVKEIKLKPEG"
BASE COUNT          579 a          751 c          656 g          528 t
ORIGIN      
        1 ggaagcaggc actcgatgga tcttggacaa tagataatgc cccaattcat gcgtcgtgag
       61 gataaagagc agcgcacctg caaacgggat tcctcgccag agcgtctcag gagatgtcag
      121 gagaaaattc aaagggccgc gcgccggacc gttatagact tggtaggctc ccgcccacaa
      181 ggtcgtaaac actgtcagga gaaaaaggac gatcggcaag gtccacgtgg aaaaaaaaga
      241 cgactctacc tcatcatcgg tctctatcgc gtcaataacc tctggagccg gttctttcgt
      301 ggtatcgaga acatgcgtgt tctctagatt tcgatgttca tgccgtccca atccatccat
      361 gccatccctg tctactgaat cgtcgcaaac ggattgtggt cgagttcttc ctcaacggtt
      421 gtggccggtc catgcccggg caggaggcga tagtcgggag agagggtcag cagacggtcg
      481 cgaaccgaat tgatgtgggt cgaatacagc tccttagggt tggaccgacc aatcgatccg
      541 gcaaaaagag tatcaccgac gaaacagacc ggaagttgtg cgtcatccag ccgataacaa
      601 atgccgcccg gcgtatggcc cggcgtcgtc aaacagtgaa ccgtacgatg tccgacgctg
      661 atcgacaatc cgtcaatcgg tgcaacgagt aaatccgtcc ggggccgcca actcaatagg
      721 ttcatatctt ccggtcccag gtacaccgga acttcgcgat agcctaaaat ctgctcgatc
      781 ccatacgcat ggtccgcatg gccatgcgtg agacaaatgc cgatcagacg cagccggtgt
      841 cgctgaagca catcaagcat ggcaggggcg ttgtacgcgg tatcgaccaa caacgcctca
      901 ccctcattat gtacaacgta cccctgtaca ccatatccgt taatagaccc atgaaccgtt
      961 tccacccagg atggcatgcg ttgggcaacc ggctcccatt tatcgatggc gatttgctcg
     1021 agcggctcag ctcgcaggcc caaggccttt gccagtgcac gaacctccgc acggtctcgc
     1081 ggctgatcgc cacgttccag cgccgtaatg tcgccgccgg gcaacccggt catccgagca
     1141 atatccccga ccgacaaccc ttggccggtg cgtgcttttt ttagaatgtc ggaaaagtca
     1201 tcttcgagcg gcatggcggc tacagtcaaa aaaggaaata tagaagtgac gaatacgaca
     1261 atatggtcca atgaagcgag tttgcttcat tgcacccacc attacccttc cggtttcagc
     1321 ttgatttctt tcacgtctcc aaacgtcgca agggctttcg gcaaggcctc tccggatccg
     1381 acggcgacga tcttcaaacg atccgggtgc aaatgtttct tggccgccgc caagacatct
     1441 tctttggtca gtcgcacgac ctgggcgcgc agctgttgaa gaaagtcctt cggcaatcca
     1501 tcgtactcga actcaaccaa tcggctgacg atcgccgatg ggctggaaaa ggaaaacacg
     1561 aatgaattga cgtacgcctc cttcgcttcc gcaagctcgg cttcggtcac cggctcggca
     1621 cgtatccgtt cgatattcgc tacgaacctc tcgatcactt cctgggtgga aaccaacttg
     1681 gtttccgccc gcatcagcca gacaccttga tcgtgcatgc ccgtattgag gcggctgccg
     1741 acggaatagg ccaggcctcg ttttgaccgc acgtcgttga acaggcgact gcgaaatgaa
     1801 ctcccaccta aaatatcgtt cgcgatggcc aacgcgacgt agtcgggatc attctccttg
     1861 atcgaaaggt gtcccacccg gagatgggtc tgtgaggtat ctttacccac gaatctgaca
     1921 accggtctgg ccaactccgc ctccggtaca tcggaaattt tcagctccgg gacagttcct
     1981 tttttccaat ctccgaatac cttgcgcaga gaagtcacca tctccgcttt gtggaagtca
     2041 ccggtcacgc cgagaatcat cccgttcggg tgaatcgtgt ggcgatggaa cgtgacgaga
     2101 tcgtctctcg tgatgcgttt gaccgaatcc agcgagcttt ctcgcgcgct cggatgatca
     2161 gccccataga gcatcttggc aaattcccgg ccgacgacgg agccgggatt atcctgacga
     2221 cggcggattg cctcgatggc ctgcaacctg gccaattcga cacgggcggg atcgaacgcc
     2281 ggcgttcgaa tgaggccggc gaagatctcc aaccctcgat tcacatcctt gcttaggaca
     2341 tcgagagacg ccgaccctga ttgtcgcccg atcccgatac tcacatcggc cgcgaactgc
     2401 tccagttcgg tatcaacctg ttccgcggaa agaccgccac cgccaccggt cctcatgacc
     2461 gccccggtca tggcagccag gccgatcttg tcgatgggat caagccaacc accc
//
LOCUS       DASTWE010000845         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1186066, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000845 DASTWE010000000
VERSION     DASTWE010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186066"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..149
                     /locus_tag="VFT30_08790"
     CDS             21..149
                     /locus_tag="VFT30_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU5092769.1"
                     /translation="MDDLGDKYGITKERTRQLEARIIKRLRDYVKKDIKDFDRLRT"
     gene            401..700
                     /locus_tag="VFT30_08795"
     CDS             401..700
                     /locus_tag="VFT30_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5092770.1"
                     /translation="MGTAEKEKKNVAKGFQPLGERVFVTYTEELDRTAGGIYVPDSAK
                     EKPQRGVVQAVGKKVENVKVGDQVLFDKYSGSKLRIEDEECLILKEEDILGIFTS"
     gene            770..>2209
                     /gene="groL"
                     /locus_tag="VFT30_08800"
     CDS             770..>2209
                     /gene="groL"
                     /locus_tag="VFT30_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5092771.1"
                     /translation="MAKQLMYGDTARAAILKGVNQLADAVKATLGPKGRNAILDKKFG
                     APTITKDGVTVAKEIELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIFREG
                     AKNITAGANPMEIKRGIDKAVEAITAELKKLSKPCQSKTEISQVGTISANNDKTIGDL
                     IAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMECSVEEPL
                     ILINEKKISSMKDLLPLLEQVAKMGKPLVILAEEVEGEALATLVVNKLRGTLNVAAVK
                     APGFGDRRKAMLEDIAILTGGQVISEDIGVKLENVKLTDLGRAKRVTIDKDNTTIVEG
                     YGDSKKIEGRVKQIKAQIEETTSDYDREKLQERLAKIVGGVAVINVGAATETEMKEKK
                     ARVEDALHATKAAVEEGIVPGGGTAYLRCVKALDGIKDVPMEQKVGLDIVRRALEEPV
                     RQIAANAGAEASVVVGKVRDDKNVNGGYNA"
BASE COUNT          617 a          577 c          637 g          378 t
ORIGIN      
        1 tgtccgacca ccccctaacc ctggatgatc tgggtgacaa atatggcatt accaaggagc
       61 ggacccgtca attggaagcc cgcatcatca aacgtcttcg tgactatgta aaaaaagaca
      121 tcaaagattt tgaccggttg aggacctgat atccccgtca ccccgccatc gccccacaaa
      181 gtactcatag actattgatt ttccaatgat ttaggatgca tgaatttctg caggcggcga
      241 caaccttcgg tattgtaaaa aatcctttga gccccctctt gacttaggtg aatcaaggcc
      301 tatctcaaag gtcacaggct gccggaatca gtaacgagtt cagcccaact ttcaaccatc
      361 aacaccaaac gagttaactt catcaggagg aggttctgct atgggtacag ctgagaagga
      421 aaagaagaat gtggccaagg gattccagcc cctgggtgaa cgtgtattcg tcacgtacac
      481 cgaggaattg gatcgaaccg ccggtggaat ttatgtgccg gattcagcga aagaaaagcc
      541 acaacgggga gtggtccagg cagtcgggaa gaaagtggag aacgtcaaag tcggcgatca
      601 agtattgttc gacaagtatt cgggcagcaa gcttcggatt gaggacgaag aatgcttgat
      661 cctcaaggaa gaagacatcc tcggcatttt taccagctag agttcaagcc acaaacaatg
      721 acgacgaact cattgataaa aaataaaaaa cgataacagg aggaacccaa tggcaaagca
      781 actcatgtac ggcgatacgg cacgagccgc catcctaaaa ggggtgaatc agctcgcgga
      841 cgccgtcaag gcgacactcg gcccgaaggg ccggaatgca attttggata agaaattcgg
      901 cgcgccgacc atcaccaagg acggcgtcac tgttgccaag gaaatcgagc tcaagaaccc
      961 gtacgagaac atgggcgccc agctggtccg cgaagtagcc agtaagacca gcgacacggc
     1021 gggggatggg acgaccaccg cgacggtgct cgcccaagca atcttccgcg agggcgcgaa
     1081 gaacatcacc gccggggcca atccgatgga gatcaagcga gggatcgata aggccgtcga
     1141 ggccatcacc gctgaactca aaaaactcag caagccgtgc caaagcaaga ccgagatctc
     1201 gcaggtaggg accatttcgg ccaacaacga caagacgatc ggcgatctca tcgcggaagc
     1261 catggaaaag gtcggcaaag acggcgtcat caccgtggaa gaagccaaat cgatgacgac
     1321 ctcgctggac gttgtcgagg gcatgcaatt cgatcgcggc tacatctctc cctatttcgt
     1381 caccaatgcg gagcggatgg aatgttccgt ggaagaaccg cttatcttga tcaacgaaaa
     1441 gaaaatcagc agcatgaagg acttgctccc gctgctcgag caggtggcca agatgggcaa
     1501 gccgcttgtc atcctggctg aagaagtcga aggtgaagcg ctggccaccc tcgtggtgaa
     1561 caagctgcgc ggcaccttga atgtcgccgc agtgaaggca cctggtttcg gcgatcgccg
     1621 caaggccatg ttggaggata tcgcaatcct caccggcggc caggtgattt ccgaagacat
     1681 cggcgtcaag ctcgagaacg tcaagctgac ggatctcggc cgcgccaagc gcgtcacgat
     1741 cgataaggac aacaccacga tcgtggaagg gtacggcgat tccaagaaga tcgaaggacg
     1801 cgtgaagcag atcaaggctc agatcgaaga gaccacatcc gattatgatc gcgagaagct
     1861 gcaagagcgg ctggcaaaga ttgtgggcgg cgtggcagtc atcaacgtcg gcgctgcgac
     1921 cgagaccgag atgaaggaga agaaggctcg cgtcgaggac gccctacacg ccaccaaggc
     1981 ggcagtggaa gaggggatcg tccccggagg cggcacggcc tacctccgct gcgtcaaggc
     2041 actcgatggc atcaaagatg tcccgatgga acagaaagtc ggactcgaca tcgtccggcg
     2101 cgcgctggag gagccggttc gacagattgc cgcgaatgca ggtgcagaag cttcggtagt
     2161 cgtcggcaaa gttcgggatg acaagaacgt caacggtggg tataacgcc
//
LOCUS       DASTWE010000846         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_636675, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000846 DASTWE010000000
VERSION     DASTWE010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636675"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFT30_08805"
     CDS             complement(<1..468)
                     /locus_tag="VFT30_08805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5092772.1"
                     /translation="MNLSAYIIAFNEAKKIADTINSVLWADEIIVADSASTDETAQIA
                     QDLGARVVQIPFQGFGHLRNQAVKACTHEWIFSLDTDEQCTPEVRDEVFMILSGTPAH
                     DAYLVPRRNYFMGRWIKHSGWYPNFRQPQLFRKGSMKYVESPVHEGYELLTAKP"
     gene            complement(465..1733)
                     /locus_tag="VFT30_08810"
     CDS             complement(465..1733)
                     /locus_tag="VFT30_08810"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5092773.1"
                     /translation="MDWRLDRVQSHARRAAGWTTTALGFTIPLWVVADSILVVLLVAC
                     WIAGGEWRERVHRMMTNPVALSVLLLFGWLLAGTLWGMGSPEERMLAVKKYADLLLIP
                     LLISMAIDAQERRRAMLALAVSLVVTLVLSLALGAGFLPTGWVINCDPSNPCAIAKPI
                     SCDQSNPCVFKKHTTHNLLMAFGALLFGVLAWKSSRRWVRWGWAGVACLAASNVLLMV
                     QGRTGYVVLAGLAMVAFHMYFGWRGMVAAVIALSVIFSAAYQVSTSFRDRVDLVARGV
                     AQWNPQTVTYDGVTERMEFFYHTIEIIQDHPLVGAGTGGFAQAYAVHAKQAGVPIPTH
                     PHNQYLLIMAQVGIIGLGLLLWLFVQQWRLTPLAGDATYGLLARGLVVTIAIGCLFQP
                     ALNDHTEKLFYCLISGLMYSGTGSQFDTRI"
     gene            complement(1901..>3007)
                     /locus_tag="VFT30_08815"
     CDS             complement(1901..>3007)
                     /locus_tag="VFT30_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5092774.1"
                     /translation="VAWWLTNCLCSPKSFLSILAHPNERTLHSMATPQTGGLAIISSV
                     VVALVAAASVLAIAQPSKPVLPKGLASGSVWIVVSMLLIFVVSFIDDCVGLPAVLRLG
                     VQAVSAFIIIGGVGLTLSSIPIPGGPNILLGLAAIPVSVLVLLWMANLYNFMDGMDGF
                     AGGMTFFGFGFLAYFGWQANFPVMLIIATFVAMGALGFLTHNFPPARIFMGDAGSITV
                     GFLAGTLMILGVRDGIFELWVPIIIFSPFIVDATVTLSRRILRRKRIWEAHREHYYQR
                     LVLSGWSHRRTVLAEYGVMILCGGLAVLYHHSTDKVRLLILGAWVGMFFLLGSLVAKL
                     ERNRKVSRSLRDPVAPGRNAEVPVQEPPRVVVKT"
BASE COUNT          799 a          861 c          757 g          590 t
ORIGIN      
        1 cggcttggca gtcaacaatt catacccctc gtggacggga gattccacat atttcatgga
       61 ccctttccga aacaattgcg gctggcggaa atttggatac cagccggaat gtttgatcca
      121 acgtcccatg aagtaattcc gcctcggtac gagatacgcg tcgtgcgcgg gagtgccgga
      181 aagaatcatg aagacttcat cacgaacctc tggtgtacac tgctcgtctg tgtcgaggct
      241 gaaaatccat tcatgtgtac aggctttgac ggcttggttt cgcagatgac cgaatccctg
      301 gaacggaatc tggacgacgc gggctcctaa gtcctgtgcg atctgagcag tttcgtcggt
      361 actggcggaa tcggccacaa tgatttcatc ggcccataag acactgttga tggtatcggc
      421 gatcttcttc gcctcattga aggcgataat ataggcggaa aggttcatat ccttgtatca
      481 aattgtgaac cagtcccgga atacatgaga ccggagatca aacagtaaaa cagtttctcg
      541 gtatggtcgt tcaatgcggg ttggaaaaga cagccgatcg cgatggtgac caccagccct
      601 ctggcaagca atccataggt cgcatcgccg gcgaggggcg tcaatcgcca ttgttgcaca
      661 aacagccaaa gcagtaaccc gagcccgatt atcccgactt gtgccatgat caaaagatat
      721 tgattatgcg ggtgagtagg gataggcacg ccggcttgtt tcgcatgcac cgcataagct
      781 tgagcaaatc ctcccgtccc agctcccacc agcggatgat cttgaatgat ttcgatcgtg
      841 tgataaaaaa attccattcg ttctgtgacc ccatcgtaag tgaccgtctg gggattccat
      901 tgcgcgacac ccctcgcgac aaggtcgacc ctatcacgga aagaggtgga aacctgatag
      961 gccgcagaaa atatcacact gagggcgatg accgctgcca ccatgccgcg ccacccgaaa
     1021 tacatatgaa aggccaccat ggcaagcccg gccagcacta cgtaccctgt tcggccttgc
     1081 accatcaata agacattgct cgcggctagg cacgcaaccc ccgcccatcc ccatcgtacc
     1141 catcggcgtg aagacttcca ggccaagacc ccgaacaaca gcgcaccgaa tgccatgagt
     1201 aagttatggg tggtatgctt tttaaataca cagggatttg actgatcaca agaaatgggt
     1261 tttgcgattg cacaagggtt ggacgggtca caattgatga cccagccggt tggaagaaaa
     1321 cccgcaccca aggcaagaga cagcaccaac gtcacgacca acgataccgc caaggcaagc
     1381 atcgcgcgac gacgctcctg ggcatcgatc gccatcgaaa tcaacagcgg gatgagcaag
     1441 agatcggcgt actttttaac cgccagcatt ctttcctccg gagaacccat cccccataaa
     1501 gttcccgcca gcaaccatcc gaacaacaac aggacgctca acgccacagg attcgtcatc
     1561 attcgatgaa ctcgttcccg ccactctcca ccggcaatcc aacaggccac gaggaggacg
     1621 acaaggatac tgtccgccac tacccacagc ggaatggtga atcccagcgc agtcgtcgtc
     1681 cagcctgccg ctcgtctcgc gtgcgattgg accctgtcaa gccgccagtc catcacctat
     1741 tggcgaagat cacggattta cgaggagatg gaatcatgta caattcccat ttcagggttg
     1801 cctcgacgaa aaaggtggcc ttcattccgc aaccggacgg cgaaatgcaa gacattcctt
     1861 gctaatcatc gatcgatccg cggcaagatc ggatttcaag ctatgtcttg acgacgacgc
     1921 gtggaggttc ttgaaccggc acttcagcgt tcctgcccgg tgcaacagga tctcgaaggg
     1981 aacgagagac ctttcgattc cgttccaact tggccaccaa gctcccaagt aggaaaaaca
     2041 tgcccaccca tgcaccaaga atgaggagtc gcactttgtc cgtcgaatga tgatacagaa
     2101 cggccagtcc tccgcacaga atcatgactc catactctgc aagcacagtc cgacgatggc
     2161 tccatccgct cagaaccaat cgctgataat aatgctctcg gtgagcttcc cagatccttt
     2221 ttcgacggag gatccgtcgg ctcaaggtca ctgtcgcatc cacaatgaat ggtgagaaga
     2281 taatgatcgg gacccacaac tcgaatattc cgtcgcgaac tccaaggatc atcaacgtcc
     2341 ccgccaagaa tccaacggta atgctccccg catcgcccat gaagatacgc gcgggcggga
     2401 aattatgggt gagaaatccc agagcgccca tcgccacgaa cgtggcgatg atgagcatga
     2461 ccgggaagtt ggcctgccac ccaaaatatg cgagaaatcc gaacccaaaa aatgtcattc
     2521 cgcctgcaaa gccgtccatg ccgtccataa agttatagag gttcgccatc cacagcagga
     2581 cgaggacgct caccggaatt gcggcaagcc caagtagaat gttcggtcct cctggtattg
     2641 ggatggaaga caaggtcaat ccgacacccc cgataatgat aaaagcggag actgcttgga
     2701 cgcccaagcg gaggaccgcg ggaagaccga cacagtcatc gatgaaggat acgacaaaga
     2761 tgaggagcat cgaaaccacg atccaaacac tccctgacgc cagcccttta ggcaataccg
     2821 gctttgacgg ctgcgcgatg gccagcacac ttgcagctgc cacaagagcg acgacgacgc
     2881 ttgaaatgat ggccaaccct ccggtctgcg gggtcgccat ggaatgcaat gtccgttcgt
     2941 tggggtgagc cagtatcgac aaaaatgatt ttggcgaaca caaacagttt gtgagccacc
     3001 acgccac
//
LOCUS       DASTWE010000847         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_766506, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000847 DASTWE010000000
VERSION     DASTWE010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766506"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFT30_08820"
     CDS             <1..600
                     /locus_tag="VFT30_08820"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5092775.1"
                     /translation="KKPAVIDKKTVQLIEKDLTQSTIVIGHPGISRTNPDFYAVTVMN
                     HVLGAGGFSSRLMDTIRDKQGLAYGITSHYDARSMPGSFWINLQTRTETTNQAITGVL
                     AEMKTIREAPVSDQELADAKAFLMGSFPLRMDSTAKLAQVLAQVEFFGLGLDYFSQYP
                     KWIERVTKEDVQRVAKQYLDPQHYALVVVGNIAKAKVRH"
     gene            621..1430
                     /gene="larE"
                     /locus_tag="VFT30_08825"
     CDS             621..1430
                     /gene="larE"
                     /locus_tag="VFT30_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEU5092776.1"
                     /translation="MQSSLIQQKLDRLRTLMTEMGSVVVAYSGGIDSTFVLKVAHDQL
                     HEKAIGITAVSPTFPSIELEAAERVAQEIGARHETVLTDQLAIDDFVRNDANRCFHCK
                     TDLYQLLGSMRQSRAAAYVVDGTNLDDLGDDRPGIKAAREWGVRSPLVEAELSKTDIR
                     ILAKELGLSNWDKPAAACLSSRIPRGIPITLEKLSRVEKAEAILHREGFRHFRVRNHG
                     EIARIELAQDELPRLMESDRCAEVSARLKALGFKFVTVDLEGYRRGGVTLN"
     gene            complement(1454..1960)
                     /gene="atpF"
                     /locus_tag="VFT30_08830"
     CDS             complement(1454..1960)
                     /gene="atpF"
                     /locus_tag="VFT30_08830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU5092777.1"
                     /translation="MPQFEPDFFSSLIFWEVVSFGILLFVLYKYAFPGILSALEEREK
                     KIKDSLDQAEQHRSEAERRLKEYEAKLNAAGKEAEAILAAAKERAQRLLDENEQRLTA
                     EAERIKGDATREIEQERRKALQDIRIQTTELALMVAEKVVQRSLTESDQRRFADEALD
                     ALSKSHPR"
     gene            complement(2036..2266)
                     /gene="atpE"
                     /locus_tag="VFT30_08835"
     CDS             complement(2036..2266)
                     /gene="atpE"
                     /locus_tag="VFT30_08835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEU5092778.1"
                     /translation="MDSAAAGLIGMGCAAAGFAGAGVGIGYIFGKMIEVVARQPEAEA
                     RVTKYMWIGFALVEAIALYGLVIAFIIMGFRK"
     gene            complement(2304..>2717)
                     /gene="atpB"
                     /locus_tag="VFT30_08840"
     CDS             complement(2304..>2717)
                     /gene="atpB"
                     /locus_tag="VFT30_08840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU5092779.1"
                     /translation="IVVTAVFSCLVYGLSLVVGFWLHGAKFLGILIPPGTPGWLVPLM
                     IPIEIISQLARPISLAVRLFANMTAGHVMLAVLFSLTISGGLLIGWLPLAISVLVYGL
                     EAALIAGIQAYIFTILTCVYLGDAFHLHGHEEHAH"
BASE COUNT          663 a          746 c          704 g          604 t
ORIGIN      
        1 aagaagccgg cggtcatcga caaaaagacc gtccagctca tcgaaaagga tcttacacaa
       61 tcgaccattg tgataggtca cccaggaatc agtcggacca acccggattt ttacgccgtc
      121 accgtcatga atcatgtgtt aggagcgggg ggcttttcat caagactcat ggacaccatt
      181 cgagacaagc aggggcttgc ctatggcatt accagccatt acgacgcccg atcgatgcca
      241 ggttctttct ggatcaacct ccagactcgc accgaaacca ccaatcaggc catcactggt
      301 gtcttggctg aaatgaagac cattcgagaa gccccggtca gcgatcagga attggccgat
      361 gccaaagcat ttttgatggg cagcttcccc ttacggatgg attcaacggc gaagctggcg
      421 caggtcttgg cacaggtgga attttttgga ttggggttag actattttag tcagtaccca
      481 aaatggattg aacgagtgac gaaagaagat gtgcagcgcg tggcaaaaca gtacctggac
      541 cctcagcact acgcgctcgt cgtcgtcggc aatattgcga aggcaaaagt tcgccactag
      601 gttcgcggag agggtacatt atgcaatcgt cccttatcca acagaagctc gatcggctcc
      661 gaacgctcat gacggagatg ggctcggtcg tggtggcata ttccggtgga atcgacagta
      721 ccttcgttct gaaggtcgcg cacgaccaac ttcacgagaa ggctatcggc atcacggccg
      781 tctcaccgac gtttccatcg attgagttgg aagctgccga acgggtcgct caggaaatcg
      841 gtgcccgcca tgaaaccgta ctgacggatc agctggccat tgatgacttc gtgagaaatg
      901 acgcgaatcg ttgttttcat tgtaagactg atctatacca acttcttggg agtatgcgtc
      961 aatcaagggc tgcagcatat gtcgtggatg gaaccaacct ggacgatctg ggtgatgacc
     1021 gccccggcat caaagccgct cgggaatggg gcgtgcgcag cccgctcgtc gaagctgaac
     1081 tgtcgaaaac cgatatccgc atccttgcca aggagcttgg actttcgaat tgggataagc
     1141 cggcagcggc ctgtctttcg tcaagaatcc cgcgtgggat cccgattacg ctggagaaac
     1201 taagccgggt tgaaaaggca gaagccattc tccatcgtga gggattccgt cattttcgag
     1261 tgcgaaatca cggtgagatc gcgcgaattg aattggcaca ggacgaactc cctcggctca
     1321 tggagtcgga tcgctgtgca gaggtcagcg cgagactaaa agcgctgggg ttcaaattcg
     1381 tgacggtgga tttggaagga tatcgtcgtg gtggagtcac cctgaactga ctccacccac
     1441 ggatcacgag acgtcagcgt ggatgcgatt tcgaaagagc gtcaagcgct tcgtctgcga
     1501 atcttcgctg atcggattcc gttaagctcc gctgcaccac tttttccgcc accataagag
     1561 cgagttcagt cgtctggatt cgaatatctt gtagcgcttt acgacgctct tgctcgatct
     1621 cgcgcgtcgc atcacccttg atgcgctccg cctcggccgt caaccgttgt tcgttttcat
     1681 cgagcaaccg ttgagcgcgt tcttttgccg ccgcgagaat ggcctcggct tccttccccg
     1741 ccgcgttgag cttggcttca tattctttga gtcgtcgttc ggcttcggac cggtgctgtt
     1801 cggcttggtc gaggctgtct ttaattttct tttcacgctc ttccaaggcg ctcaagatgc
     1861 cggggaatgc atatttgtag agtacgaaca agagaatccc gaacgagacg acctcccaga
     1921 aaatcaacga agaaaaaaaa tcaggttcaa actgtggcat agttcatatc agaaaggctg
     1981 aggccgaggc tgaattaaga atttcgctcc accttgacct acacctctcc tgctcctatt
     2041 tccggaagcc catgatgatg aaggcgatga ccaagccata cagcgcaatg gcttctacca
     2101 gcgcgaaccc gatccacatg tacttcgtga cgcgagcttc tgcttcaggc tggcgtgcta
     2161 ccacttcaat catttttccg aagatgtagc cgatccccac accggccccg gcaaatcctg
     2221 ccgcggcaca tcccataccg atcaaccctg ctgctgctga atccattatg tcttactcct
     2281 ccctttggct aaacctgcac gcgctaatgc gcgtgctcct catggccatg taaatgaaac
     2341 gcgtctccca aatagacgca cgtcaagatg gtaaaaatat aggcttgaat tcctgcgata
     2401 agggcggctt ccaatccata gacgagaacc gaaatcgcta agggcagcca cccgatcaac
     2461 aatccgccgc tgatcgtgag gctgaacagc accgcgagca tcacatggcc tgccgtcata
     2521 ttcgcaaaga gccgaacagc cagtgaaatg ggtcgcgcta actgactgat gatctcaatc
     2581 ggaatcatca acggcaccag ccatccgggc gtaccaggtg gaatcagtat ccccagaaac
     2641 tttgctccat gtaaccagaa tccgacgacc aagctaagac cgtacaccag gcaggagaag
     2701 acagccgtca caacaat
//
LOCUS       DASTWE010000848         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_669273, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000848 DASTWE010000000
VERSION     DASTWE010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669273"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..>1093)
                     /locus_tag="VFT30_08845"
     CDS             complement(255..>1093)
                     /locus_tag="VFT30_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092780.1"
                     /translation="CHVRDGVILGPFEDAKAPHPTKYDPSLRTTQVCHRCHSVVGGPA
                     QFYNGGPCGTYAEFEDGYWMKERGFICQNCHMPEIERPIAVGGPIRQGRQHLWRGGHD
                     PEMIKRAMGVKVVADPAQPKPGDKVRITLTLINAGAGHKLPTGDPDRHFTVEFAVEDQ
                     NGKVLEDQSDTMGRWIMWQPAIIELHDNRLMPLASRDYTFEYQLPKDSVGLKLITRVR
                     YHIQTERQHQMLIDKYGLTAKDPYNFTVYEGQVPLTGNLTDAFKHTGQNTHLACAAPG
                     HS"
BASE COUNT          226 a          297 c          308 g          262 t
ORIGIN      
        1 ggagtacatg cgggctcatc ataatggatc ggaggcaaaa atcgctatgc gcgttcgccg
       61 agtatcgact gaacagcatc gtgatggata tgagtcgaac aaactcagaa agttggcaat
      121 ggagggacta ccagcggaac cagaaacgtc gctttccttg cagccaggaa ggaaacaggt
      181 cgacgagtct tgatagcttc gcgatgaagg tggtcaactt ggaatatgat tgcttgatcc
      241 gagtactatg attctcagct gtgacccggt gcggcgcagg cgaggtgagt attctgtcca
      301 gtgtgcttga aggcatccgt cagattgccg gttaagggca cctgcccttc atacaccgtg
      361 aagttatagg gatctttcgc cgtgaggccg tacttgtcga tgagcatctg atgttgccgt
      421 tccgtctgaa tatggtaccg cactctcgtg atcagtttga gcccgacact gtccttcggt
      481 aattgatatt caaacgtata gtcccgactg gccaacggca tgagccgatt atcgtggagt
      541 tcgatgatcg ccggttgcca catgatccat cgccccatcg tatccgactg gtcttccaac
      601 acctttccgt tttgatcctc gacggcaaac tcgaccgtga agtggcgatc cgggtcacca
      661 gtcggaagtt tatgcccggc gccagcattg atcaacgtca gcgtgattct gaccttgtca
      721 ccaggtttag gttgggccgg gtcggctacc accttcacgc ccatcgcccg cttgatcatc
      781 tcggggtcgt gtccgccacg ccagagatgt tgacgtccct ggcggatggg acctcccacg
      841 gcgataggcc gttcgatctc cggcatatgg caattttggc agatgaaccc gcgttccttc
      901 atccaatagc cgtcttcgaa ttctgcatag gtgccgcatg gtccgccgtt atagaactgc
      961 gccggccctc ccaccacgct atggcagcga tgacagacct gggtcgtgcg aaggctgggg
     1021 tcgtacttcg tcggatgggg agctttggca tcctcgaatg gtcccaggat cactccgtct
     1081 cggacatggc atg
//
LOCUS       DASTWE010000849         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1509450, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000849 DASTWE010000000
VERSION     DASTWE010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509450"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VFT30_08850"
     CDS             complement(<1..1297)
                     /locus_tag="VFT30_08850"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092781.1"
                     /translation="MPNPRNHNPSTGHLIIEGARQNNLKNVSLQIPHNKVTAITGVSG
                     SGKSSLAFDTIFAEGQWRYVESLSTYARMFLDKVARPDVDRLLNVRPAIAIEQKNQVR
                     TARSTVGTTTEIADLLRLIFAKIGKPTCPDCHREARSFQPDTVADDVLKRWPDARAMV
                     LFPIATPSSKEEPAFIQSLLTRGFTRVKTTDNVLDLHEIGHSQLHRSPSLHIVLDRLV
                     IREDNRTRLVEAVETAFREGEGRCSFDIIDHGRQSYSTRFLCQSCGRTFEPLKPILFS
                     FNHPLGACPECKGFGNVLRYDPALVIPDPTKSLTQEVIEPWSKPSSAWWQKQMLLAMK
                     RHKVDITAPFKSLPKDTQRLLWEGDKSFDGIHDFFEYLEGKRYKLHVRVFLSRYRTPF
                     DCPGCHGSRLKPDALFVKIAGADIHQTMDRTIESLSEWTE"
BASE COUNT          331 a          379 c          470 g          337 t
ORIGIN      
        1 attctgtcca ttctgaaagg ctctctatgg tccggtccat cgtttggtgg atgtcggccc
       61 cggcaatttt gacgaacagc gcatccggct tgaggcgact gccatggcaa ccggggcaat
      121 cgaagggtgt gcgatagcgg ctgaggaaga cgcggacatg cagcttatag cgcttccctt
      181 cgaggtactc gaagaaatca tgaatcccgt cgaaggattt gtcgccctcc cagagcaatc
      241 gttgcgtgtc tttcggcaga gacttgaagg gagccgtgat atcaaccttg tgccgtttca
      301 tcgccaacaa catctgtttt tgccaccagg cggagctggg tttgctccag ggctcgatga
      361 cttcttgggt gagcgacttg gttgggtcgg ggatgaccag cgccggatca taccgcagca
      421 cgttgccgaa ccctttacat tcaggacagg cgccaagcgg atggttgaac gaaaagagaa
      481 tcggcttcaa gggttcgaag gtccgcccgc agctctggca gagaaatcgc gtgctgtagg
      541 attggcgtcc atggtcaatg atgtcgaacg aacagcggcc ctctccttca cgaaacgccg
      601 tttcgactgc ttcgacaagt cgagtgcggt tgtcctcccg gattaccaga cgatcgagga
      661 cgatatggag cgaaggagag cgatggagtt gtgagtgtcc gatctcgtgc aaatcaagca
      721 cattgtcagt ggtcttaact cgggtgaacc cgcgtgtgag aagtgattgg ataaaagcag
      781 gctcttcttt ggaggaagga gtcgcaatgg ggaatagtac catcgcccgt gcatcgggcc
      841 atcgcttgag gacatcgtcg gctactgtgt cgggctgaaa cgaacgggct tctcgatgac
      901 aatcgggaca ggtgggtttg ccgatcttgg cgaagatcag gcgcaggaga tcggcgattt
      961 cagtcgtcgt accgaccgtc gagcgggctg tgcgcacctg attcttctgc tcgatggcaa
     1021 tggccggtcg gacattgagg aggcgatcca catccgggcg agccaccttg tcaagaaaca
     1081 tacgggcata ggtcgagagc gactcgacat accgccactg gccttctgca aagattgtgt
     1141 cgaatgcgag tgaagattta ccggatcccg acacgccggt aatggccgtc actttattgt
     1201 gcggaatctg cagcgagaca ttcttcagat tattctgccg ggccccttca ataataaggt
     1261 gaccagtcga gggattgtgg ttccgagggt tcggcatagc aatcgctcct gcatcgggat
     1321 gaccgatcat cgtagcaaca gtcggaggct ggttcaatga aggaacaggg ggagagctac
     1381 gggttcttgt ctttatagga cgaaagcgta aaatccgccg atcgtgctat cccggcgtgc
     1441 cgacgtgcaa tgaggccttt cggttctttc tgaagcacgg tccgagggtt ggaccaacgt
     1501 cggggccacg caggggc
//
LOCUS       DASTWE010000850         1427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1154097, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000850 DASTWE010000000
VERSION     DASTWE010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154097"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..427)
                     /gene="hypA"
                     /locus_tag="VFT30_08855"
     CDS             complement(86..427)
                     /gene="hypA"
                     /locus_tag="VFT30_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEU5092782.1"
                     /translation="MHELSVTESLLKIAVEHAEKAKAGRVTDLHIVIGDLASMVDDSI
                     QFYWDSIAKDTIAEQATLHFRRVPAELQCNTCSARYQPTDEELICPNCHGIGATIVSG
                     EEFFLESIDVE"
     gene            complement(437..931)
                     /locus_tag="VFT30_08860"
     CDS             complement(437..931)
                     /locus_tag="VFT30_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEU5092783.1"
                     /translation="MKQLLLLGYGNPDREDDGVAWHILRALTRKLGLAAPGSYEDEFP
                     EFALIDFAFHLQLTPEMAEDISAYESVCFIDAHTGNIPEPVRLIEVESEFQASPFTHH
                     LTPQSLLSMCETIYGRKPDAALVSVLGHRFLFSRQLSAETEKLVPDAVELIWGWMKTR
                     QILE"
     gene            complement(1056..>1427)
                     /locus_tag="VFT30_08865"
     CDS             complement(1056..>1427)
                     /locus_tag="VFT30_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5092784.1"
                     /translation="FAALQVMCEGNTGAVLVTEEEKIVGIFTERDYAREGEVKGRSAK
                     ETLVSELMTSQMVLIKPETTLEECAELMGKYHVRHLPVLEKDRVIGVVSIRRLAEGLI
                     QQEQGTISELENYILGTGYGE"
BASE COUNT          325 a          374 c          370 g          358 t
ORIGIN      
        1 aggatgtttt cgacaacggg gatattttga ggcatgtagg gctcctaaca agtaaacagt
       61 gctgtgttgt actactggta taaactcatt ccacatcaat ggattcgaga aagaattctt
      121 caccggagac gatcgttgcg ccaatcccgt ggcagtttgg acagatcagt tcttcgtcgg
      181 tgggttggta cctcgcagag caggtattac actgtaattc ggcaggcact cggcggaagt
      241 gtaaggttgc ctgctcggcg atcgtgtcct tggcgatgct atcccagtaa aactggatcg
      301 agtcatccac catgctggcg aggtcgccga tcacgatgtg caggtccgta acgcgtcccg
      361 ctttcgcttt ttctgcgtgt tccacggcta tcttcaatag agattctgta accgataatt
      421 catgcattgc aaaatgttat tccaggatct gtctggtttt catccagccc cagatcaatt
      481 caaccgcatc aggtaccagc ttttctgttt ccgcagaaag ctgtcgtgaa aagagaaaac
      541 gatgacccag cacggaaacc agggctgcat ccggttttct tccgtagatg gtttcgcaca
      601 tggagaggag tgattgcggt gtaagatggt gggtaaatgg cgaagcttga aactcgctct
      661 cgacctcgat caagcgcacg ggctcgggga tatttccggt gtgcgcatct ataaagcaaa
      721 cggactcata ggcactgatg tcttctgcca tttcgggagt caattgcagg tgaaacgcga
      781 agtcgatcag ggcaaactcc ggaaactcgt cttcatagga cccgggtgct gccaaaccaa
      841 gtttgcgcgt gagcgcacgc agaatatgcc atgcgacgcc gtcatcttcg cgatcggggt
      901 ttccataacc cagcagcagt aattgtttca agtctaatac ctttgatact atgtttaaag
      961 agacagacca gacacaagga atgccagtgc cctcctgtat ctggtccgcc agattcggct
     1021 ttcgagatcc tgagactggg atattccttg aagcgttact cgccgtagcc ggtcccaagg
     1081 atgtagtttt cgagctcgct gatcgtgccc tgctcttgct ggatcaggcc ctcagccagt
     1141 cgtcggatgg agacaacccc gatgacacgg tctttctcga gcacggggag gtggcgcaca
     1201 tggtacttgc ccatcaattc cgcacattct tcgagcgtgg tctcgggttt gatcaagacc
     1261 atctggctcg tcatcaactc cgaaaccaga gtctctttgg cagacctgcc cttgacctcg
     1321 ccctcgcggg cgtagtcacg ctcggtaaag atcccaacga tcttttcttc ttccgtgacc
     1381 aggaccgcac cggtatttcc ttcgcacatc acctgaaggg cagcaaa
//
LOCUS       DASTWE010000851         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_863009, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000851 DASTWE010000000
VERSION     DASTWE010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863009"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFT30_08870"
     CDS             <1..180
                     /locus_tag="VFT30_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092785.1"
                     /translation="AFLDSDCVEQQEKLDGTVAWVRPWEKKGFLIGVVWDELVTKEKN
                     RWLYYYLEETLKASV"
     gene            complement(366..2207)
                     /locus_tag="VFT30_08875"
     CDS             complement(366..2207)
                     /locus_tag="VFT30_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5092786.1"
                     /translation="MLQIESISKQYSTKVLLTEASVHLRPNSRVGLVGPNGAGKTTLF
                     RMVLGEESPDKGSIRKRPRLRIGYLPQELETITGKTAVDAAHRDEYPEHEAKRILMGL
                     GFTEGDFDRPVEKLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFEQFLLQ
                     SGMTLLIISHDTAFLDRVVTHIWELRHHKIEEYRGNYSLFCKLKAEKDAQLHASATRQ
                     SKEIARVQKFVDRFRYQANKASQVQSRLKQLEKVKRIEIQRDAKRVKFRFPLPSASGR
                     QVLDLAGASKRYGEKVVYKTLDFSVERGQRIALVGENGAGKSTLLKMLAGVLPLDTGT
                     RTVGHGVSLHYFAQHQAETLNPEHSILESLEEVTKHAEMNFLRGIAGAFLFSGQDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDVLTDALAEFPGTIIFIS
                     HDPTFLARIATRVVEIEEGQARNYIGDYEYYLWKKAQEFESIKETSEELEGAAQHTSS
                     GPTRAMAQQVQPKARGGERRDLTKTQARLEKQVSRAEAEITEAETRIKAREAELADPK
                     LYAEFDRWNLLHQEQETWQRDLERLTARWESLSAELEGVKQKLGALY"
     gene            complement(2507..2582)
                     /locus_tag="VFT30_08880"
     tRNA            complement(2507..2582)
                     /locus_tag="VFT30_08880"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2547..2549),aa:Ala,seq:ggc)
BASE COUNT          619 a          761 c          741 g          675 t
ORIGIN      
        1 gccttcctcg attcagattg cgtcgagcaa caggagaagc tcgacgggac cgtcgcctgg
       61 gtcaggccct gggaaaagaa aggattcttg atcggggtcg tctgggatga attggtgacg
      121 aaagagaaga accgctggtt gtactactat ctagaagaaa cgctcaaagc ctccgtctag
      181 tagggcattg aaacgtccta tcagcttcgt tctcgtttcg ctcagatttc tcgacatacg
      241 gctcaccatc tcaccggcgc gcacaaacat ggcgctcttt attcatcgcg ccgtgcgcct
      301 cattattttt cgcgtcgtgg cttcactgca gcctgctgct gagtcttttg aacgccctgc
      361 aagggttagt acaatgctcc cagcttttgt ttcacccctt ccagttctgc cgacagcgat
      421 tcccagcgag cagtcagtcg ttcgagatcg cgctgccagg tctcctgttc ttgatgcagg
      481 agattccaac gatcgaactc ggcgtagagt ttcggatcgg ccaattccgc ttcgcgcgcc
      541 ttgatcctgg tctcagcctc ggtaatttct gcctccgctc gggacacctg cttttccaat
      601 cgtgcctggg tcttggtcag gtcgcgccgc tctcctcctc gcgcttttgg ctgaacttgt
      661 tgtgccatcg cgcgggtagg gccggaagac gtgtgttgtg cggctccctc gagttcctcg
      721 ctcgtttcct tgatcgattc gaactcttga gcctttttcc acaggtagta ctcataatcg
      781 ccgatgtagt ttcgggcctg tccctcttcg atctcaacga cccgggttgc gatgcgcgcc
      841 aaaaacgtcg ggtcatggga aatgaagatg atggttcctg ggaattcggc caatgcatcg
      901 gtgagcacat ccaccgaggc cgggtccaaa tggttcgtcg gctcgtcaag aagcaaggta
      961 ttggctggtt cgaccagcat gcgggctaag gccacccggt tccgctcacc accgctcaaa
     1021 gccttgatcg gttttttctg atcctggccg gaaaacaaga aggcgcccgc aatcccacgc
     1081 agaaaattca tttccgcatg tttggtgact tcttcaagag attcgagaat cgagtgttcg
     1141 gggttcaagg tttcggcctg atgctgagcg aagtagtgta agctcacccc gtgccccaca
     1201 gttctcgtgc ctgtatcgag aggaagcaca ccggcgagca tcttcaagag agtgcttttt
     1261 cccgctccgt tttcaccgac cagcgcgatg cgttgaccgc gctccacgga aaaatcgagt
     1321 gtcttataga ccaccttttc accataacgt ttactcgctc cggcgagatc caatacttgg
     1381 cggccgctgg ctgacggaag gggaaatcgg aacttgactc gttttgcatc tcgttggatt
     1441 tcaatccgtt tgaccttttc caactgcttc agtcgcgatt gaacctgact ggccttgttg
     1501 gcctggtacc ggaagcgatc gacgaacttt tgaacgcggg caatctcttt ggactgtcga
     1561 gtcgccgagg cgtgcagctg agcatctttt tccgctttca acttgcagaa aagcgaatag
     1621 ttgcctcggt attcctcgat cttgtgatgc cgcagttccc agatatgcgt gaccacgcgg
     1681 tctagaaaag cggtgtcgtg gctgatgatc aggagcgtca taccggattg taaaaggaac
     1741 tgttcgaacc aacgctgggt cggcttatct aaatggttgg tcggctcgtc gagcatcaac
     1801 acatcggggt tcgataagag cagatgcgcc agcgcgaccc gcatccggta gcctccggac
     1861 agtttttcaa ctggacgatc gaaatcgcct tccgtaaatc ctaaccccat gaggatgcgc
     1921 ttggcttcgt gttcaggata ttcatcgcga tgggcggcat cgacggcggt ctttccggta
     1981 attgtttcga gctcctgtgg gagatagccg atccggagcc gaggccgctt gcggatcgag
     2041 cctttgtccg gcgactcttc acccaggacc atgcgaaaga gcgtcgtttt cccggcgccg
     2101 ttcggtccga ccaagcccac tcgtgaattc ggtcgaagat gcacggaggc ctcagtgagc
     2161 agcaccttcg tggagtattg tttgctgatg gattcaattt ggagcatcgg gacaaacctt
     2221 ccatgtgaaa tgggacgact gtgtcaccgg aaatgaactc gaacgaagtg aacagttagg
     2281 aggtcggaag gcgaggcaaa gtgaggaaat taaacacggc aaacattcta gaatccaaat
     2341 cttaagacac ttgcgacgtc tatccggctg cgacgattac gataaacctc aaaaagagcg
     2401 acacgtgcaa gctatatcag gaaactgtaa aacaattgtg ggttttatcc gtcggctcaa
     2461 ttacaaaaac ccttaaaata gccgacggca cgcacggagt gcggtttggt ggagctggcc
     2521 gggattgaac cggcgacctc gtgaatgcca ttcacgcgcg ctcccaactg cgccacagcc
     2581 ccaccccttg ggatagtcag agatcaaata atatggaaaa ttgcgacgtt gaacaattct
     2641 ggcatgctaa cacgcagtgt atagccagtc aaccgaccaa tcgctgcctc acaatgtcat
     2701 gaacccgagc aaagctttga atacgaagta tctcaatgac caacttgaca aaagaatagg
     2761 ctacacctac catgaggccc cttggctctg atcgcc
//
LOCUS       DASTWE010000852         1284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1477249, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000852 DASTWE010000000
VERSION     DASTWE010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477249"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFT30_08885"
     CDS             <1..393
                     /locus_tag="VFT30_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5092787.1"
                     /translation="GSAVVSDVIAIGRNLLKGAIGRVPVASFQQDQRRPLRLKPMEEI
                     SSLYYLRFTVVDRPGVLAQIAGELGRCGISISSMVQQGRREGQTVPVVIKTHTARERD
                     VQSALREINRKAFVSEPTRLIRVEGKDE"
     gene            397..>1284
                     /gene="thrC"
                     /locus_tag="VFT30_08890"
     CDS             397..>1284
                     /gene="thrC"
                     /locus_tag="VFT30_08890"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5092788.1"
                     /translation="MNRWRGVIEEYRKFLPVTEKTPVINLGEGNTPLIRATRLAKTIA
                     PGVELYLKFEGGNPTGSFKDRGMTLAISKAVESGARAVMCASTGNTSASAAAYGARAG
                     LAVYVLIPAGKIAMGKLSQAMMHQATVIQIEGNFDQALTLVKDFSATMHIELVNSVNP
                     YRIDGQKTAAFEVCDQLGDAPTLHVLPVGNAGNITAYWKGYKEYRAANQTMRVPRMMG
                     FQAAGAAPIVLGRIVEQPQTIATAIRIGNPASWSTALKAVEESAGAIDMVTDDEILQA
                     YTTVAATEGVFCEPASAASV"
BASE COUNT          294 a          342 c          394 g          254 t
ORIGIN      
        1 gggagcgccg tggtcagtga cgtgattgcg atcgggcgga atctgctcaa aggcgccatc
       61 ggtcgagtac cggtggcgtc gtttcaacag gatcagcgcc gaccgcttcg actgaaaccg
      121 atggaagaga tcagctcgct ctattatttg cgattcacag ttgtggatcg acctggtgtg
      181 ttggcccaga tcgccggcga attgggccgt tgtggaatca gcatttcctc tatggtgcaa
      241 caaggacgcc gcgaagggca gacagtgcca gttgtcatta aaactcatac ggcaagggag
      301 cgcgacgtcc aaagcgcgct tcgcgaaatc aatcggaagg ctttcgtctc cgaaccgacg
      361 aggctcattc gtgtcgaagg caaggatgaa tgatcgatga atcgttggcg tggagtcatt
      421 gaggaatacc gaaaattcct cccggtcact gagaagaccc cagtcatcaa cctgggagag
      481 ggtaataccc ctctgatcag agctaccaga ctggcgaaaa cgattgcacc gggagtcgaa
      541 ctctacctga agtttgaggg gggcaacccg accggttcgt tcaaagaccg aggcatgaca
      601 ttggccatct cgaaggcagt ggagagcggc gcacgagccg tgatgtgtgc gtcgaccggc
      661 aacacctccg cctcggctgc cgcgtacgga gcacgtgccg ggttggctgt ctacgtgttg
      721 atcccggccg gtaagatcgc gatgggcaaa ctctcccagg cgatgatgca tcaagcgacg
      781 gtgattcaga tcgaaggcaa ctttgatcag gccctgaccc tcgtgaaaga cttttcggcc
      841 accatgcaca tcgaactcgt gaactcggtg aacccgtacc ggattgatgg gcagaagacc
      901 gccgcctttg aggtgtgcga tcaactcggc gacgctccga ctcttcatgt attgccggtg
      961 gggaacgccg gaaatattac ggcctattgg aaagggtaca aggaatatcg cgcagcgaat
     1021 cagacgatga gagtgcctcg catgatgggc tttcaagccg caggtgccgc tccgattgtg
     1081 ttgggcagga ttgtcgaaca accgcagacg atcgccacag ccatccgaat cggcaacccc
     1141 gccagttggt ccacggcctt gaaagccgtg gaggaatcag caggagcgat cgatatggtg
     1201 acggatgatg agattctcca ggcctacaca accgtggcgg ccacggaagg agtcttttgt
     1261 gagccggctt ctgccgcgtc cgtc
//
LOCUS       DASTWE010000853         1321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_636824, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000853 DASTWE010000000
VERSION     DASTWE010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636824"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFT30_08895"
     CDS             complement(<1..483)
                     /locus_tag="VFT30_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092789.1"
                     /translation="MAIVNECVGLDIGQTGLKAVRFRRRLSGRETIDYFQHPMPYSRP
                     EDLEPARRVKSLRGFLWRNGLYSTDRLVTAIPCQDLFVRTLSFPFKDSAKLAQVIPFE
                     VENLVPMPLEDLAIGSLVLPPGQTAEGTLRESKGSDVLVTAAPRDKVAEHLQFLAQAD
                     V"
     gene            complement(602..>1321)
                     /gene="gspN"
                     /locus_tag="VFT30_08900"
     CDS             complement(602..>1321)
                     /gene="gspN"
                     /locus_tag="VFT30_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HEU5092790.1"
                     /translation="DWTMGLPLGLEWRNVTLSQPNWAPIQLTVLQAKLGVMKALNGTL
                     GLDVVAKLNDTASPTGLAKGTVTASSLSLEGPVTIKGQVQQVDLSKLLRRYVTHGMLS
                     GDFSHRVDSGQTTATAMKGEGMWTAEATDLVIDQIPLGNGRALSLTFSKVSVGLACRD
                     LRCDVTQLKGDGIDGSFTGEGHITIQRPMQQSQLNLTVTVVPGPGFASKAGTLGLPSP
                     PPGTPMTVKIVGSLAQARIAL"
BASE COUNT          269 a          369 c          391 g          292 t
ORIGIN      
        1 cacatcggct tgggccagaa actggagatg ctcagcgacc ttatctctcg gtgcggccgt
       61 aaccaggacg tccgaaccct tgctctcccg gagtgtgcct tctgccgtct gtcctggcgg
      121 caacaccagg cttccgatcg cgaggtcttc gagcggcatc gggacgagat tctcgacctc
      181 aaagggaata acttgggcga gcttggccga atccttgaat ggaaaggaaa gagtcctgac
      241 gaaaaggtcc tggcaaggaa tcgcggtcac caaccggtct gtgctataga gtccgtttcg
      301 ccagaggaat ccgcgcagcg acttgacacg tcgagccggc tcgagatcct cggggcggga
      361 atacggcatg ggatgctgga agtaatcgat cgtttcgcgc ccgctcaggc gacggcggaa
      421 acgcacggcc ttcaagccgg tttgtccgat gtccagcccg acacattcgt ttacaatggc
      481 catgaaccca gcttccgcat tcgttttgtg attatggttt tggatttcac gtgcgagtga
      541 atagcatcag gttaactgaa actgcaacat accatccgcc acctgaaacg tcagccgtac
      601 tttacaacgc aatcctcgcc tgtgccaagc ttcctacgat cttgacggtc atgggcgttc
      661 ccggaggtgg cgaggggagg cccagtgttc cagctttcga agcaaatccg ggaccgggga
      721 cgactgtcac cgtgaggttc aattggctct gttgcatcgg tcgctggatg gtgatgtgtc
      781 cttcgcccgt aaaggagccg tcgatgccgt cgcccttcag ttgcgtcacg tcgcagcgga
      841 ggtctcggca tgcgagtcct accgagacct tgctgaatgt gagtgaaaga gctcgtccgt
      901 ttccgagtgg aatctgatcg atcaccagat ccgtcgcttc ggccgtccac ataccctcac
      961 ccttcatcgc ggtggcggtt gtctgaccgg aatcgactcg atgggagaaa tctccgctga
     1021 gcatgccgtg cgtgacgtag cggcggagga gtttggaaag gtccacttgc tgaacctgcc
     1081 ctttaatcgt gacggggcct tccaaggaaa gagaggaggc ggtgactgtg cctttggcga
     1141 gacctgtcgg agatgcggta tcgttcaact ttgcgacgac atcgagtcca agcgtgccgt
     1201 tcaaggcttt catgactccg agtttggcct gcagcaccgt caattgaatc ggggcccagt
     1261 ttggttgcga caacgtgacg ttccgccatt cgagacctaa agggagcccc atggtccaat
     1321 c
//
LOCUS       DASTWE010000854         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1347987, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000854 DASTWE010000000
VERSION     DASTWE010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347987"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFT30_08905"
     CDS             <1..480
                     /locus_tag="VFT30_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092791.1"
                     /translation="AGGHYFPREELPDLVAWFNRQRREPLPTRLTVVRDGSHFQAFDW
                     VRLDATDPIAAFSEDLVDKRDERIKRRVYAKLDASIAGSNRIEVKTEHVQRYSLFLNE
                     QLIDFSKPLAVVTNGRVSFEGTVMPSVETLLRQARLRQDPERLFSVHLTIAIVKPAS"
     gene            477..1292
                     /locus_tag="VFT30_08910"
     CDS             477..1292
                     /locus_tag="VFT30_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092792.1"
                     /translation="MIPARRFRPGPVTHMLVVCIGIGIFVPAGYGRSEACTLSPDHAG
                     LTFPVERMDPVWTCTLQAIVQSYTTVSKVGPIQTALSESMYRHLLDHPPFTADVIRRL
                     HLGLYRSEARGPGRFWGDDGEGTKGIVELVYEDPTSRIYFLEGIHESRLLPHVMGKAV
                     VFLRIGVVQDANGNEAMDSTLVAYTKLDNRFLSGLVSLLHPLVSKIVTSRLQKGVETV
                     DQLGVAMKQDPQRVMAELANPPSLAEHHLTFLKRALRSQLSTDDGSSDGRSIP"
     gene            1289..>1602
                     /locus_tag="VFT30_08915"
     CDS             1289..>1602
                     /locus_tag="VFT30_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5092793.1"
                     /translation="MTTSRSFVLICTVGIFCFISYNMVRMPALSLFAESLGASPERIG
                     LIVSVSTLTGVFLKLPSGALSDIYGRRFLLRIGVVAFGLPPFLYPFITNLDALTALRF
                     LHG"
BASE COUNT          321 a          431 c          454 g          396 t
ORIGIN      
        1 gcaggcggac actatttccc cagagaggag ttgccggacc ttgttgcctg gttcaatcgt
       61 cagcgccgtg aaccgttgcc gacgcgcctc accgtcgtgc gtgacgggag ccactttcaa
      121 gcgttcgact gggtccggct cgatgcgacc gatccgattg cggcattttc agaagatttg
      181 gtggacaaac gcgacgaacg aatcaagcgc cgggtgtatg ccaagcttga cgcgtcgatt
      241 gccggaagca atcgcatcga ggtcaagacc gagcacgttc agcggtatag cttatttctc
      301 aacgagcagt tgatcgattt ctccaaacca ctcgcggtgg tgacgaacgg tcgtgtctcg
      361 ttcgaaggaa ctgtcatgcc gtcggtcgaa accctattga gacaagccag attacgacag
      421 gatcctgaac gactcttctc tgttcatctg acgattgcca tagtgaagcc ggcttcatga
      481 tccccgcacg gcggttccgg cctggtcctg tgacacacat gcttgttgtg tgtatcggca
      541 tagggatttt tgtgcctgct gggtatggcc ggtcggaagc atgcacgttg tcccccgatc
      601 atgccgggct cacatttccc gttgagcgca tggatccggt ctggacctgt acactgcaag
      661 ccatcgtcca gagttatacg actgtgagca aagtggggcc gatccaaacc gctctatctg
      721 aatcgatgta tcggcatctg ctggatcatc ctccctttac agctgatgtg atccggcgcc
      781 tacatcttgg tctttaccgg tcggaagcgc gaggtcctgg ccgcttctgg ggcgatgacg
      841 gcgagggaac aaaaggcatc gttgaattgg tatacgagga ccccacgtcc cgcatctact
      901 ttcttgaggg aattcatgaa agccggttgc ttcctcacgt gatggggaaa gcggtcgtct
      961 ttctcaggat aggtgttgtg caagacgcga acggcaatga agcgatggac agtacattgg
     1021 ttgcgtacac caaattggat aatcgttttc tttcagggtt ggtttccctc ctgcacccgc
     1081 ttgtttccaa gattgttaca agcaggttgc aaaaaggagt agagactgtc gatcaattgg
     1141 gggtggcgat gaagcaggat ccccagcggg tgatggctga actggcgaat ccgccctctt
     1201 tagcggaaca tcacctaacg tttctgaaac gagcgctgag gagccaactg agcaccgacg
     1261 acggtagttc ggacggtaga tcgatcccat gacgacgtca cgcagtttcg tgctgatctg
     1321 cacggtcggc atcttttgct tcatcagcta taacatggtg cggatgcccg cgctctcctt
     1381 gttcgccgaa tcgctcggtg ccagcccgga acggatcggt ctgatcgtgt ccgtttcgac
     1441 tttaacgggc gtctttttaa aactgccttc cggcgccctc tccgatattt atggaaggcg
     1501 gttcttgctg cggatcggcg tggtggcgtt cgggctgcct ccatttctct atcccttcat
     1561 taccaacttg gacgccctga cggcgctacg gtttcttcac gg
//
LOCUS       DASTWE010000855         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_830834, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000855 DASTWE010000000
VERSION     DASTWE010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830834"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFT30_08920"
     CDS             complement(<1..284)
                     /locus_tag="VFT30_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092794.1"
                     /translation="MARLIAVSIDCRNEAVRQTFEEIVSRRRDYLITRGKGTGTADMV
                     LLDLDESRPQQTFAKVRELLIAAPDLEVFLTAARTDPHMLLEAFRIGVKE"
     gene            complement(311..>1024)
                     /locus_tag="VFT30_08925"
     CDS             complement(311..>1024)
                     /locus_tag="VFT30_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092795.1"
                     /translation="WNPTTRVHTPTVNQPKAVRVWARRDSSANGAISTFLANVIGISS
                     ISVRAVATADLTAVGQTAPGQLDVPFAISEYYFTEFGCGDVIKFYPNDGTPQACAAWH
                     TFDQSPANANRLRTIIDGMIDGSYQSPATVPGDSLNAINGNVASAFPNLINLYNSKKD
                     ANGNWDVFVPVYESPDCAPPTGAIPIIGYAEARITNVEGAPNFQISATILCNIFEGNT
                     VGGGPPFGPVLSTVPGLVE"
BASE COUNT          213 a          304 c          283 g          224 t
ORIGIN      
        1 aattccttga cgccgattcg gaacgcttcc agcagcatgt gcgggtccgt ccgcgccgcc
       61 gtgagaaaca cttcgagatc cggggcggcg atgagcaact cccggacctt cgcgaacgtc
      121 tgttgcggcc gcgattcgtc caaatcgagc aagaccatgt cggccgtgcc ggttcctttc
      181 cctcttgtga tgagatagtc ccgccgtcgc gaaacgatct cttcgaaggt ttgccggact
      241 gcctcattcc gacagtcgat cgacactgca atcagtcgag ccatgcgagc tgtcccttcc
      301 caatttcaga ttattcgaca aggcctggaa cggtcgatag aaccggcccg aacggagggc
      361 cgccccccac agtgttcccc tcgaagatgt tgcacagaat cgtcgccgag atttgaaagt
      421 tcggcgcgcc ctcgacgttt gtgatccgag cctcggcata cccgatgatg ggaattgcgc
      481 cagtaggcgg agcgcaatca ggggattcat acaccggcac gaagacatcc caatttccat
      541 tggcgtcttt cttcgagtta tacagattga tgaggttggg gaaggcgctg gccacgttgc
      601 cgttgatagc attcagcgaa tcccccggca ccgtagcagg cgattgatag gaaccgtcga
      661 tcatcccatc gatgatggtt ctcaacctgt tcgcattggc cggagactga tcgaaggtat
      721 gccacgcggc gcaggcttgc ggcgttccgt cattgggata gaacttaatc acgtctccac
      781 agccgaactc agtaaagtaa tactccgaga tcgcaaaggg cacatccaac tgaccgggcg
      841 ccgtttgtcc cacggctgtc aaatccgccg tcgccaccgc tctgacgctg atgctcgata
      901 ttccgatcac gttggcaagg aacgtactga tcgcgccatt ggccgaagag tctcgtctcg
      961 cccacacccg tactgcttta ggttgattga cggtgggggt atggaccctt gtcgtcggat
     1021 tcca
//
LOCUS       DASTWE010000856         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_540142, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000856 DASTWE010000000
VERSION     DASTWE010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540142"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1366
                     /gene="secY"
                     /locus_tag="VFT30_08930"
     CDS             47..1366
                     /gene="secY"
                     /locus_tag="VFT30_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU5092796.1"
                     /translation="MLERLLTSFQNIFKIPELRTRVLFTLGMLVVYRIGSHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVIPHLT
                     KLAKEGERGRKKIIQYTRFGTIGIALIQGFGIAVGLEQMNQGAFVLNAGWGFRLMTVI
                     TLTAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPAAVAQTYNLYEIGQLNAFLL
                     IALALLMVGVVAAIVFLESGRRKIPVQYAKRVIGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWVKSIGAQLAPGSLLYTLMYVGLIIFFCFFYTAVVLNPV
                     DMADNMKKYGGFIPGIRPGQRTSDYIYNVLTKITFAGAIYLAVVCVIPEFLIYKLNVP
                     FYFGGTSLLIVIGVGLDTAQQIESHMLMRNYEGFLGKGMAPLRGRSG"
     gene            1371..2027
                     /locus_tag="VFT30_08935"
     CDS             1371..2027
                     /locus_tag="VFT30_08935"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU5092797.1"
                     /translation="MRLVFLGAPGVGKGTQADKAAAQYRIRKISTGDLLREAVRNKTS
                     LGLEAKGHMDQGRLVPDSVVIGLVREKLADPSHANGFILDGFPRTVPQAEALTKVLVE
                     RGLQLDRVINFKVSREEIVKRLSGRRSCPKCQATYHLEFAPSKRGTLCERCGEELVQR
                     SDDQREAIEMRLRVYEEQTAPLINFYEKQRLLSHLNGAETVETVYHNLVKALAAPQTA
                     "
     gene            2024..>2690
                     /gene="map"
                     /locus_tag="VFT30_08940"
     CDS             2024..>2690
                     /gene="map"
                     /locus_tag="VFT30_08940"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU5092798.1"
                     /translation="MIILKTPAEIAVMAEASRVVAEALAIVKKAVCPGISTDELDRIA
                     EEAIRARGAVPAFKGYRNYPKTLCASVNEQVVHGIPSKRKLKEGDIIGLDLGAIVGGF
                     YGDSAITVAVGRIREETAKLVHVTEEALYLGIKQAVVGNRLTDISNAVQQHVESAGFS
                     VVTEFVGHGIGRQLHEEPQVPNYGKPGQGPRLQPGMVLAIEPMVNMGRSAVRVLEDRW
                     TAVT"
BASE COUNT          582 a          613 c          815 g          680 t
ORIGIN      
        1 gaagcaaaga tccaagcagc cggagggcga gtcgaggtca tcggcggtgc ttgaacggct
       61 cctgaccagt tttcagaata tcttcaagat ccccgagctg cgtactcgtg tcctcttcac
      121 gctcggaatg cttgtggtgt atcggatcgg gtctcacatc ccgactcctg gtatcaatgg
      181 tgaagctctt tctgagtttc tacagaaaca gggcggggcg ttgctgggat ttctcgacat
      241 tttctccggc gggtcattat cccgtctgac gatctttgcg ctgggcatta tgccgtacat
      301 cagcgcatcg atcatcctcc aattgttgac ggtcgttatc ccgcatttga ccaaattggc
      361 caaggaggga gaacggggtc ggaagaaaat cattcagtat acccggttcg ggacgattgg
      421 tattgccctg attcaaggat ttggtattgc agttgggtta gaacaaatga atcagggagc
      481 gttcgtgctc aatgccggat ggggattccg tctcatgacg gtcatcacat tgacggccgg
      541 cacgggtttc ctgatgtggc tcggtgaaca gatcactgag cgaggaatcg gcaacggaat
      601 ctctttaatc atttttgccg gtattgtcgc ccggctacct gcggccgtgg cgcaaaccta
      661 caatctgtat gaaatcggcc agcttaatgc gttcttattg attgcattgg cactattgat
      721 ggtcggcgtt gtcgccgcga ttgttttttt agaaagcggg cggcggaaaa ttccggtgca
      781 gtacgcaaag cgggtgatcg ggcgacgggt ctatggagga cagagcacgc acattccgtt
      841 gaagatcaat acggcagggg tcattcctcc catattcgcc tcgtcgatca tcgcgtttcc
      901 tgcgaccatt gcggggtttt ttgagacgcc atgggtgaaa tcgattggag cgcagctggc
      961 accgggatcg ctgctctaca cgttgatgta tgtggggctc atcatctttt tctgcttctt
     1021 ctataccgcg gtggtcctta accccgtcga tatggccgac aacatgaaaa agtacggggg
     1081 ttttattcct ggtatcaggc ctggtcaacg aacttctgac tatatctaca atgtcctgac
     1141 gaagattaca tttgcgggcg ccatctatct ggccgtcgtc tgtgtgattc ctgagttttt
     1201 gatttataag ctgaatgttc ccttttattt tggcggtaca tccctgttga tcgtcattgg
     1261 tgtgggtctg gatacggccc agcaaattga atcccatatg ttgatgcgga attacgaggg
     1321 atttttaggc aagggcatgg ctccattgcg cgggagaagc ggttaggaac atgcggcttg
     1381 tgtttctcgg tgccccgggt gttgggaaag gcacacaggc tgataaagct gctgctcagt
     1441 atcgcattcg taaaatttcg accggtgatt tgcttcggga ggcggttcgg aataagacat
     1501 cccttggcct tgaagctaaa gggcatatgg atcagggaag attggtcccc gattcagtcg
     1561 tcataggact ggtacgggag aaattggctg atccatcaca tgccaatggg tttattctcg
     1621 atgggtttcc aaggacggtt cctcaagcgg aagcgcttac gaaggtgctt gtcgaacgag
     1681 gtctccagct ggatcgagtg attaatttca aggtttctcg agaggaaatc gtgaagcggc
     1741 tgagcgggcg tcggagctgt cccaagtgtc aggcgaccta tcacttagag tttgcaccat
     1801 ccaagcgcgg cactctctgt gagcggtgcg gtgaagagtt ggtacagcga agtgatgatc
     1861 agcgagaagc gattgagatg cgcctccgtg tgtacgaaga gcagacagcg ccgttgatca
     1921 acttttatga gaagcagcgg ttattgtcac atcttaacgg tgccgagacg gttgaaacgg
     1981 tataccacaa cctcgtgaaa gcgctcgcgg caccccagac ggcatgatca ttctgaaaac
     2041 gcccgccgag attgcggtga tggcagaggc gtcaagagtg gtggctgaag cgctcgcgat
     2101 tgtgaagaaa gcggtctgtc caggtatcag cacggatgag ctggatcgta ttgccgagga
     2161 ggcgatacgg gccagggggg cggttccggc gttcaaaggc taccggaact atcccaagac
     2221 cctctgtgct tccgtgaacg agcaggtcgt tcacggtatc ccttcgaagc gaaagctgaa
     2281 ggagggggat atcatcgggc tggatctcgg tgctattgtg ggtggattct atggggattc
     2341 agcgataaca gtggcggttg gccggatccg ggaagagacg gcaaaattgg ttcatgtgac
     2401 ggaagaggcg ctctatcttg ggatcaagca ggctgtggtc ggcaaccgtt tgacggatat
     2461 ctcgaatgct gtgcaacaac atgttgaatc tgccggcttc tcagttgtca ccgagtttgt
     2521 ggggcatggg attggccggc aattgcatga agagcctcag gttcctaact atgggaagcc
     2581 tgggcagggg cctcgattgc aaccaggtat ggtcctcgct atcgaaccaa tggtgaatat
     2641 gggtcggagt gccgttcgtg ttcttgagga tcggtggaca gcggtcacgg
//
LOCUS       DASTWE010000857         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_23101, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000857 DASTWE010000000
VERSION     DASTWE010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23101"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFT30_08945"
     CDS             <1..1023
                     /locus_tag="VFT30_08945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5092799.1"
                     /translation="VEARWEVIAGTQEFFAVVKRLTDALQGRDEKITEDMYQTFREIN
                     ERNAKALNLEADMVVIHDHQPAGMVEYRARGTWLWRCNLDLSQPQRRAWTFLRRYALK
                     YDAAIFSLSSFAQRLPIPKFIVYPSIDPLSPKNREMTRSEINQIVDRLGIPRTKPIIL
                     QVAKFERFKDPLGAIQAYRIVKKHHDCRLVLAGSGVMHDPEGEAVLAEAREAAARDPD
                     IHLVQLPPEADLEINALQRIATIVLQKSIKEGFGVTVSEAMWKGKPVVGGNAEGIAAQ
                     IIDEVTGYVVHSAEGAGFRLRHLLNNPGLITRMGAAGREHVRRNFLITRQLGDFLTLL
                     KIMPPQ"
BASE COUNT          307 a          336 c          406 g          255 t
ORIGIN      
        1 gtagaggctc gttgggaggt cattgcagga acgcaggagt tctttgcggt cgtgaaacgc
       61 ctcaccgacg ccttgcaagg acgagacgag aagatcaccg aggacatgta ccagaccttc
      121 cgggagatca acgagcggaa cgcgaaagcg ctgaacctcg aagcggacat ggtcgtgatc
      181 cacgaccatc agccggccgg catggttgag tatcgggcac gaggaacgtg gctgtggcgc
      241 tgcaacctgg atctgtccca accgcaacgg cgcgcgtgga cgtttttgcg gcggtatgca
      301 ttgaagtacg atgcggctat tttttcattg tccagctttg cgcaacgcct gccgatcccg
      361 aaattcatcg tgtatccttc gatcgatcca ctgagcccga agaatcggga aatgaccaga
      421 tccgaaatca atcagatcgt ggaccggttg ggtatcccgc gaaccaaacc gatcatcctg
      481 caagtggcaa agttcgagcg gttcaaggat ccgctcggcg ccatccaggc gtatcgcatt
      541 gtgaaaaagc accacgattg ccgattggtg cttgccgggt ccggagtcat gcacgacccg
      601 gagggtgaag cggtgctggc cgaagcgcgc gaggcggccg cacgggatcc tgatattcat
      661 ctggtgcagt tgccgccgga ggcggatctg gagatcaacg cattgcaacg aatcgctacg
      721 atagtactac agaagtccat caaggaagga ttcggcgtga cggtctcgga agcgatgtgg
      781 aaagggaagc cggtggtcgg aggaaacgcc gaagggatcg cagcgcaaat catcgacgaa
      841 gtgacgggat atgtcgtcca ttccgcggaa ggggccggat tccgcttgcg tcatttattg
      901 aataatcctg gattgatcac ccgcatggga gcagcggggc gggagcacgt gcgccggaat
      961 ttcctcatca ccagacaatt gggcgatttc cttacattgc tgaagatcat gccgccgcag
     1021 tgatggggga agttctgaat gctgggctat gaagaggtcg tggtatcaat gagtcgacga
     1081 agcactgttc caaccagcac ctcacgcgtt acgccccacg gttagaccga tgaccgacga
     1141 gtcaatcatg actgaatcag tcgagcacgt ctccaaagag gcggtaactg ccgaggtcgt
     1201 cgtgggactg ttgacactga acaacgctgg cacgattgag caagtggtga agagcatcat
     1261 cgaaggactg caacgattct tcgccggtgc ttcggcgatg ctcg
//
LOCUS       DASTWE010000858         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1315626, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000858 DASTWE010000000
VERSION     DASTWE010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315626"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFT30_08950"
     CDS             <1..534
                     /locus_tag="VFT30_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEU5092800.1"
                     /translation="AHGYLLHEFLSPLSNQRQDEFGGRLEDRARFPLRITETVRRVWP
                     EQLPVFVRISATDWMEGGWDLVQSITFSGWLRDLGVDLIDCSSGGLVPGAKIPIEPGY
                     QVEFSAAIRKQAGIATGAVGMITEPGQAESILSNGQADVVFLARALLRDPYWPLHAAK
                     ELNAEVSWPVQYLRAKD"
BASE COUNT          269 a          291 c          337 g          268 t
ORIGIN      
        1 gcgcacggct acctgctgca tgaattcctt tcacctctct cgaatcaacg ccaggatgag
       61 ttcggaggcc gtctcgaaga tcgcgccaga tttcccttgc gaattaccga aacggtacgg
      121 cgggtttggc ctgaacagct gccggtgttt gtccgcatct cagcgactga ctggatggaa
      181 gggggatggg atctggttca atcgatcacg ttctcaggct ggttaagaga tctgggagtt
      241 gatttgattg attgttccag cggtggattg gtgccaggcg cgaagatccc gattgagccg
      301 ggctaccagg ttgagttttc ggcagctatt cgaaaacagg cgggcatcgc aaccggagcg
      361 gtcggaatga tcaccgagcc tggtcaagcg gagagcatcc tatccaacgg gcaggcggat
      421 gtcgtgtttc tcgcacgagc gttgctgcgc gatccctact ggcctctcca tgctgccaag
      481 gaactcaacg ccgaggtctc ctggcccgtg caatatctga gggcgaagga ctagacgagt
      541 ctcagaacag agaagtcgtc gttcaggaat tcgtttctga aacggagatg catcgtcccg
      601 gctggagcgt cctatgaatg ggtggggacc aaaggaaaaa ataccactga acatcgccag
      661 gcgagatggg aagtccctat caatggtggg actgtataac tatggaaccc aaccgcctcg
      721 ctaaggaggc caatgccgac gttgaccgtc atcactgtcc caccgaacca atggatggct
      781 cgcatccaca agcgaacgcc agggatattg caggatgatg aagtggacac atggcggaat
      841 cgcggcctat cctatccaga gcttctcacg tatgtgctaa agctcctccc gagaattttc
      901 tggaatgcca tcccgtggac aagagttatt gaattcaggc cgcattgaca cactgagtgt
      961 gctggcaaca tcggctggat gatgcgccgc tgttgcgcga atgaatgtga ttggtcgtct
     1021 tcgcatctgc tttcggggct cggtacgtcg caaatgaaca gtccaagagc gtcgatgatt
     1081 tcctgctcgt aaagtgtgct gattctgata tactgaactt ctatctcaat ggttggagct
     1141 acttaatgga agacaatgat cggtt
//
LOCUS       DASTWE010000859         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_507915, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000859 DASTWE010000000
VERSION     DASTWE010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507915"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFT30_08955"
     CDS             complement(<1..651)
                     /locus_tag="VFT30_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092801.1"
                     /translation="MNSSIAEVPAPAVKNLASLVGHYIETHDSNEADQLLQLILSDKS
                     ASVETVSHIIMAGRTYQNQPVGSLPNEQIVIRGQTYPLSLFIPPTYQASRAYPLVVCL
                     HGFGFTGEEYLERWQSRLGEDYLLACPTYPSGAWFTRRAEELVLQTIRQAGHRYHVDP
                     GRVFLTGMSNGGIGAWLIGMHHAPLFAGLAPMASGLDDVLMPFLANLRNTPVYIIHG"
     gene            complement(699..1820)
                     /gene="ribD"
                     /locus_tag="VFT30_08960"
     CDS             complement(699..1820)
                     /gene="ribD"
                     /locus_tag="VFT30_08960"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5092802.1"
                     /translation="MQANRAQDLHYMTLALRLAAKGQGTTSPNPMVGAVVVRHGRIIG
                     QGFHLRAGAPHAEILALRQAGKLAQGATLYVTLEPCCHLKKRTPPCVPEILRSSVRRV
                     VIAMHDPNPSVKGKGAAALRRAGLSVTVGVARSGAEELNKSYCHWMKTGRPYVTLKTG
                     MTLDGKMATSTGESRWITGGPSRREVHQLRCCVDAVLIGVDTVLVDDPSLTARIGPRF
                     KKLASRQPLRIVVDSRLRTPFKAQVLTQQDKAKTIIATTAAAPAARRSVLQKKGIEIL
                     TLPTQQSRVSLPALLKQLGRRGITSLLVEGGSEVNTAMLKAKLVDHIRLYMAPLLLGG
                     QNAKSMIGGESPARLADAIEVRHVVTRYIGADVVVEGDL"
BASE COUNT          478 a          628 c          619 g          487 t
ORIGIN      
        1 tccatgaatg atatagacag gcgtgtttcg aagattagct aaaaacggca tcaacacatc
       61 atccagtccg ctcgccatag gagcaaggcc ggcaaacaat ggcgcatgat gcataccgat
      121 cagccacgca ccgatcccgc catttgacat gccggtgaga aaaacccggc cagggtcgac
      181 atgataccga tgtccagctt gccggatcgt ctgcagcacc agctcctcag cacgcctggt
      241 aaaccacgca ccagagggat aggtgggaca ggccagtaga tagtcctcac ccaatcgtga
      301 ttgccaacgc tccaaatatt cttcgccggt aaaaccgaat ccatgcagac acacgactaa
      361 ggggtaggct ctcgaagctt ggtacgtcgg cgggatgaac agagacagcg ggtacgtctg
      421 tccacggatc acgatctgtt cattgggaag actcccgaca ggctggttct gataggttcg
      481 tccagccatt atgatgtgag aaactgtttc gacggacgcg cttttgtcgg agagaatgag
      541 ttgcaacaac tgatcggctt cattactgtc atgggtctct atgtagtggc ctacgagtga
      601 agcaagattc ttgaccgccg gtgccggcac ttctgcaatg ctggagttca ttccgctact
      661 aagaaccatc gtcaagctga tgaatcttat ccaggcggtc acaggtctcc ttccaccaca
      721 acatcggctc cgatgtaccg tgtcacgaca tgccgtacct cgatcgcatc tgcaagccgt
      781 gccggactct ctcccccgat catactcttg gcattctggc cgcccagaag caacggcgcc
      841 atgtagagac ggatgtgatc caccaacttt gccttgagca tcgccgtgtt gacctcactc
      901 cctccctcca cgagtaagga cgtgatacca cgccgtccga gctgcttgag tagagcaggc
      961 aatgaaacac ggctctgctg ggtgggcaac gtgaggatct ctatgccttt tttttgcaga
     1021 accgatcgtc gagccgcagg ggctgcagca gttgtggcaa taatcgtttt ggccttatcc
     1081 tgttgtgtca ggacctgtgc cttgaatgga gtgcgaaggc ggctatcgac cacaatgcga
     1141 agaggctgcc gcgatgctaa ttttttgaat cgcggtccta ttctggccgt caatgacgga
     1201 tcatcaacca gaacggtgtc gaccccgatc aaaacggcat cgacacaaca gcgaagttga
     1261 tgcacctccc ggcgagacgg cccgcccgta atccatcgcg actctcctgt tgacgtggcc
     1321 atttttccat caagcgtcat tccagtcttg agcgtcacat agggacggcc cgttttcatc
     1381 caatggcaat acgatttgtt cagctcttcg gccccagagc gggcaactcc gaccgtgacc
     1441 gagagtccgg cccgccgaag cgccgcggct ccttttccct ttactgatgg attcggatca
     1501 tgcattgcga tcaccacgcg gcgaacgctc gagcgaagaa tttcagggac acaagggggg
     1561 gtccgcttct tgagatggca gcagggctca agcgtcacgt acaatgtggc tccttgagcg
     1621 agtttccctg cctgccgtag cgcgagaatt tctgcgtgtg gagccccggc tcggaggtga
     1681 aaaccttgcc caatgattct gccgtgacga accactacgg ctccgaccat cggattcggg
     1741 cttgtcgttc cctggccctt cgcggccaag cgaagggcca gagtcatgta gtgcaagtcc
     1801 tgagcgcggt tagcctgcat tgtttacgag ggttcgtggc gaatctgccg agacgccatg
     1861 tgaggtccgc ggctcgacga ataagtcgag ccgcgtttgc ggacgccggc gagatggtga
     1921 gccggcggtg tcttggcgcg tggaaccatg aggaagggag gcatacttga aacagtatgt
     1981 tgaccgaccg agtggcaagc ccatctgctg gcatgctaga tgagtacatg ccagcttctc
     2041 gcaacagcgt atcggcggtt gcagcagaaa ccttcgtgaa taatgcaggc ctctcaccgt
     2101 gccttgcggg ccacgcggga tcggcgggaa tttgatcgcg acttcgtcgg cttgacccgt
     2161 gatgccttgg tggatttgct tgctcttttc gtatcttcct cggccagagc cg
//
LOCUS       DASTWE010000860         1330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1477381, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000860 DASTWE010000000
VERSION     DASTWE010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477381"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFT30_08965"
     CDS             <1..398
                     /locus_tag="VFT30_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092803.1"
                     /translation="DGKEQSFPIRSQIRSKVAAIPIGVKALFLLDETNQVADVAFKNP
                     ENMRKGNRHEPALTPAKNPHERVSGTIVESSEDHHSQITVRMDSGSDRTYEVRETSQK
                     KLANLKKGDQVVLLIDSEKKVIDVAIPPK"
     gene            complement(1099..>1330)
                     /locus_tag="VFT30_08970"
     CDS             complement(1099..>1330)
                     /locus_tag="VFT30_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092804.1"
                     /translation="IGMIEPFKEKAQEVKAQSVAMANTTIKNLATAAWWMFVLAMLSL
                     FATVGGGALGVSENLRLELDGRPVIRPAHAGA"
BASE COUNT          350 a          374 c          311 g          295 t
ORIGIN      
        1 cggatggcaa ggagcaatcc tttccgatcc gttcccagat tcgcagcaaa gtggcggcca
       61 ttccgattgg agtgaaagcc cttttcctcc tcgacgaaac caatcaagtt gcagatgtgg
      121 cgttcaagaa tcccgaaaat atgaggaagg gaaaccgtca cgaacctgcc ctcactcctg
      181 ccaaaaaccc gcatgaacga gtcagcggga cgatcgtgga atcttcagaa gatcaccata
      241 gtcaaatcac cgttcgaatg gacagcggat ctgataggac ttatgaagta cgagagacat
      301 cccagaagaa actggcgaat ctcaagaaag gtgaccaagt cgtgcttctt atcgatagtg
      361 aaaaaaaagt gatcgatgtg gcaattccac cgaaataatc ggaaaccgcc acgcgcaatc
      421 aggcggtcct gggcggtagc aagagtagtc agttcctcaa tacgatgact ggagcgatct
      481 aatctcgatc ctggacagga atgtgacccc gagcttttca gaattcgggg tcacatacga
      541 tctgagtgcc atgactcacc gaaaattatt tgaattctca aaaggccgct ttcagtttct
      601 ttctcgcgtt gccagtcgag attcacgcgg accggtcaga gtgaagccta tcaaaaacct
      661 gctcagtcca gagcgctgat atgggaaaca catgattcac tgggagagtg gcgtgaccgg
      721 ggtctctaag gttgaactaa gaaagtgttc aacattcgac agctgatgaa aacccttcct
      781 aagcacggct acctgatgat acgaccctcg tcggcacaga ccaatctcac ataagcgtat
      841 accgcacgag atccgccgcc ttcttccgaa cgcagcttaa ggcccattcc tgcccctacc
      901 ctccgacaat cggcttttta tggaactgtt gccctacctt gcggcgaata tctgaaagcc
      961 cggaggccaa agagagcctc ttttgctctc ttcggcctac catgacactc tcgacacaag
     1021 gctacgctgc agcagtacgg cagtttaaca atgcattatc aattcgccgc cgacacggct
     1081 ctcttttgtt tctcttcctt acgcacccgc gtgcgccggt ctaatcactg gtcgtccatc
     1141 gagttcaagc ctcagattct ccgaaacgcc taaggctccg ccacccaccg tggcaaagag
     1201 cgaaagcatg gccaacacga acatccacca ggctgcggta gcaaggttct taattgtcgt
     1261 atttgccatg gcaacggact gcgccttcac ctcctgagcc ttttccttaa acggctcgat
     1321 cattccgatg
//
LOCUS       DASTWE010000861         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_281464, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000861 DASTWE010000000
VERSION     DASTWE010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281464"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFT30_08975"
     CDS             <1..356
                     /locus_tag="VFT30_08975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5092805.1"
                     /translation="TLTPLTKDHTLIEKYLERGILTAETAKTHPERHVLTRALGVGAT
                     VKPTITAFPILPEDMVLLCSDGLTKMLEDEDVRTVFADGELDPTQVCNRLVAAALERG
                     GEDNVTVVVVAPRSS"
     gene            439..1317
                     /locus_tag="VFT30_08980"
     CDS             439..1317
                     /locus_tag="VFT30_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5092806.1"
                     /translation="MPRRGSFVCGNLLLLIGLTTIAIAGEPSDPEAAIRQMVRANAEK
                     DLPTLSRLMAHDADIVSYSVGGRKYVGWPEFEREMREEFINTQKIEIPITELKVWTKG
                     DLAWFTMELDYTRYVGEGSAVKRTVLPLRETGVLERRAGRWQLLSWHESFRSAQLGGP
                     LATPSTSAGSQKFASNAAAAVVPDVSGEWDILEVEDEKRYKATLDKNGNGPYTQQGGR
                     FITTKIENRLWQGTWHQPGNDREGGFEVLLSEDGTQAKGIWWYTRVDTRKNIPPKEHG
                     GTYHWKKVTPSPASAQ"
     gene            1398..>2341
                     /locus_tag="VFT30_08985"
     CDS             1398..>2341
                     /locus_tag="VFT30_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092807.1"
                     /translation="MRRSLTLFISIFFMVSSAYADGGHDHHAIPTLKKQVGSKITYGE
                     NTATLTFGPIDLPAGHGEGDMGDSMPRFHFQLPEDKYLVGFKSALSTVDGKELPRNYL
                     HHILMINNTRPSVSCPGEPLFFGGAGLEMSETQFPDGYGVKLSATDKLMTVVAFYHGA
                     PPTKNVIATFTMYFAPKIKPVKEMEIYQVGVNIVCFTKFGDRPADQTDEGIEIGPGVQ
                     VRKAPLKFSMDGCVKYAYPHGHDELLLIALENKTKRQTLLRTVPDVEQDGTLREFLPH
                     QVYKDIQGFPISKEDDYEMVMVYHHSLQKTEFQHGMGN"
BASE COUNT          571 a          642 c          617 g          511 t
ORIGIN      
        1 gcacactgac ccctctgacc aaagaccata cattgatcga aaaatacctt gaacgcggca
       61 tcctcacggc agaaacagca aaaactcatc cagaacggca tgtgttgacg cgggccttgg
      121 gggtaggcgc aacagtgaaa cctactatca cggcttttcc catactgccg gaggacatgg
      181 tactactctg ctcggatgga ctgaccaaga tgttggaaga cgaggatgtc cgaaccgtgt
      241 tcgctgacgg cgaactcgac ccgactcagg tctgcaaccg cctcgttgcc gcggcgctcg
      301 agcgcggcgg cgaggacaac gttaccgtgg tggtggtcgc tcctcgttcg tcctgatttt
      361 acttcgttgt tgacatggcc tatcaggccg tgtagttttc ctgctcattt gaactttgtc
      421 acctacacgg agacctttat gccccgtcgg ggatcgttcg tctgtggaaa cctgcttctt
      481 ctcatcggcc ttacgacgat tgcgatcgcc ggtgaacctt ctgacccgga agcagctatt
      541 cgccaaatgg tccgggccaa tgcggaaaaa gacctcccga cattatcccg ccttatggct
      601 cacgatgccg atatcgtcag ttactcagtg ggtggtcgga aatacgtcgg ttggccggag
      661 ttcgaacggg aaatgcgcga ggaattcatc aacacccaga agattgagat tccgattacg
      721 gagctcaaag tttggacgaa aggcgacttg gcctggttta caatggagct cgactatacc
      781 cgctatgtcg gcgaaggatc cgcagtgaag cggacggtac ttcccttaag agaaaccggt
      841 gtcctcgaac gtcgcgccgg ccgttggcag ctgttatcgt ggcacgagtc atttcgatcc
      901 gcccagctag gaggtccgct tgccacgcct tcgacatccg cgggatcgca aaaattcgcc
      961 agcaatgctg ctgccgcagt ggtgcctgac gtgagcgggg aatgggatat tctcgaagtc
     1021 gaggacgaaa agcgctacaa ggcgacgtta gacaagaatg ggaacggccc ctatacgcaa
     1081 cagggcggac ggtttattac aacaaaaatt gagaaccgac tgtggcaagg cacttggcac
     1141 cagcccggca atgatcgtga aggcggattt gaggtactcc tctcggaaga cggcacgcag
     1201 gcaaagggaa tctggtggta cacacgggtc gatacgagaa aaaacattcc gccgaaggag
     1261 cacggcggca cctatcactg gaagaaagtg acaccatcac cggccagcgc ccaatgagcc
     1321 agtttgtttc ttcccaacca taccaagaac tcggagtccg gtttaacatc accgcttcac
     1381 ttcctctggg aggtcttatg cgtagatccc tcactctttt tatctctatc ttcttcatgg
     1441 tctcatcagc ctacgctgat ggggggcatg atcatcatgc catccccacg ctgaaaaagc
     1501 aggtgggctc aaagatcacc tatggagaga acaccgcgac tctgaccttc ggtcccatcg
     1561 atcttccagc cggccacggt gaaggcgata tgggcgattc catgcctcga ttccatttcc
     1621 agttgcctga agacaagtat ttggtcggct tcaaatccgc tctttccaca gtcgatggga
     1681 aggaattgcc gagaaattac ctgcaccata ttttgatgat caacaatacc aggccgagcg
     1741 tctcctgccc cggtgagccg ctcttcttcg gtggagccgg acttgaaatg tccgaaacgc
     1801 aattccccga cggatacggc gtgaagctgt cggcaaccga caaattaatg acggtcgtcg
     1861 cgttctatca cggcgccccg cccaccaaaa acgtcatcgc aacgttcacg atgtattttg
     1921 cgccgaagat caaaccggtc aaggagatgg agatctatca ggtcggcgtc aacattgtct
     1981 gctttacaaa attcggcgat cggcctgccg atcagacaga cgaaggcatc gaaatcgggc
     2041 cgggtgtcca agtccgtaag gccccattga agtttagtat ggatggctgc gtcaaatacg
     2101 cctatccgca tggccacgac gaattgctct tgattgcatt ggagaataag accaagcgac
     2161 agaccctcct ccggactgtg ccggatgttg agcaggatgg aacacttcgg gaattcttac
     2221 cccatcaagt ctataaagac atccagggat tccccatctc taaagaagat gactatgaga
     2281 tggtgatggt ctaccaccat tctctccaga aaactgaatt ccagcatggc atgggcaatt
     2341 a
//
LOCUS       DASTWE010000862         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_23208, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000862 DASTWE010000000
VERSION     DASTWE010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23208"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..582)
                     /gene="nuoK"
                     /locus_tag="VFT30_08990"
     CDS             complement(280..582)
                     /gene="nuoK"
                     /locus_tag="VFT30_08990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5092808.1"
                     /translation="MIPLSAYTAVSAVLFMTGLLGVLIRRNFIIVLMSVEIMMNAANI
                     NLVAFSHYLESMAGQLVALFIIAIAAGEAAVGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            complement(579..1106)
                     /locus_tag="VFT30_08995"
     CDS             complement(579..1106)
                     /locus_tag="VFT30_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5092809.1"
                     /translation="MVGMFFSYFALVSIAAGVLTVTLRHPVHCALSLLVLLMHVSGLF
                     ILLNAEFLWAVQVIVYVGAILVLYLFVLMLMNLKTDERYFHPSAPYILGPVVLGASYV
                     LYLLLRSPFSGAKGDTPATAVLQDGDTYAVGIKMFSDHLLQFEIVGIFLLGAIIGAIV
                     LAKAPKPLDTERDRL"
     gene            complement(1116..1703)
                     /gene="nuoI"
                     /locus_tag="VFT30_09000"
     CDS             complement(1116..1703)
                     /gene="nuoI"
                     /locus_tag="VFT30_09000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU5092810.1"
                     /translation="MSVAALTKKILHAALFYEILDAMKVTFRHMLHRPMTFQYPREQR
                     TIPDAHRGALGLLRYDDGQERCVGCDLCEVACPSHCIKVISAEDTARPLQRYASEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEYSTHDKRSLMFDKKRLYDIGERHLDDGKKYL
                     YAHNQEKNVEQSREYRYYFPQSVQKSTQSPPKHLS"
     gene            complement(1737..>2626)
                     /locus_tag="VFT30_09005"
     CDS             complement(1737..>2626)
                     /locus_tag="VFT30_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5092811.1"
                     /translation="ETSLPPVGSGAHLIEILKRCKSGQIKALYILGENPLATLPASME
                     VRAALERLELLVVQDPFLTDTGKLAHFVLPACTYAEKDGTFTNLEGRVLRIRQAMDPL
                     GESLPDWHIMTALANAMGCQWEYQSANDIQAEIMKLLPGYYNLGQPRKIVPVPDQYLS
                     NGYAAEVKARYQQTPASEERTRPFSLLMGQLLVHSGKLSAQAPGLIKIAPNTGKLRMN
                     IGDMERLGLQDGTKVRLTSDRGSLQLGVQPDQFIAPGTCFFPEHFNEPPVKDLMPVSV
                     DATTGVPSFKQIWVSIEQA"
BASE COUNT          667 a          720 c          690 g          549 t
ORIGIN      
        1 gagaaacgat agaccgaccg acccccaagc caatcgatgg gccatgtcat gagagtagcg
       61 atgcccgagc agcccattca caaggaccgc cagcagcggg aaaacaggaa tcaatttgat
      121 gaggagatca atcaagtcgg acacagtatc gtgccattcc gaggatgctc agctagccaa
      181 ccatgctgca cggagccgga acctaaagcg tactcttggg ggtacgttga ggggtacgag
      241 ccaccgactg caccgttgga tgtgattgtc cacatcctgc taccacttta ataggttcat
      301 ttcatccaca ttcgtggaaa tcttccctct gaacacgaca atgatgatgg ccaaaccaac
      361 ggccgcttca cccgccgcaa tggcgatgat gaacagagcc acaagttgcc ctgccataga
      421 ttccaaatag tgtgaaaagg cgaccaaatt gatgttggcc gcattcatca tgatttcaac
      481 cgacatcagc acaatgatga agttccgcct gatgagcaca cccaaaagcc ccgtcataaa
      541 aaggacggcg cttacggcgg tataggcaga caggggaatc ataaccgatc tctctccgta
      601 tccagcggct tcggagcctt cgccaggacg atcgcgccga taatggctcc gagcagaaag
      661 atgccgacga tctcaaactg caagagatgg tcgctgaaca tcttgatacc cacagcgtat
      721 gtatctccgt cttgcagcac ggcagtggcc ggagtatccc ccttcgcccc actaaacgga
      781 gaccgcagga ggaggtacag aacgtagctt gcccccaaga cgacaggtcc gagaatatac
      841 ggagccgagg gatggaaata acgttcatcc gttttgagat tcatcaacat caacacaaag
      901 aggtacagga ccagaatagc cccaacgtag acgatgacct gcaccgccca aaggaactcc
      961 gcgttcagga ggatgaacag gccggagacg tgcatgagca acacgagcag ggagagcgcg
     1021 cagtgaacag ggtgtcgcaa ggtcactgtc aaaacaccgg cggcaatact aaccaatgca
     1081 aaataagaga aaaacattcc gaccatgtgc gcggctcagc tcagatgttt gggaggtgat
     1141 tgggtcgact tctgtaccga ctgcgggaaa tagtagcggt actcgcggct ctgctcgaca
     1201 tttttctcct ggttgtgcgc atacaaatac ttctttccat catcgaggtg gcgttcgccg
     1261 atgtcgtaca gccgcttctt gtcgaacatc agactgcgct tgtcgtgagt cgagtattcg
     1321 tacatcttgg tcatcgccaa tgcgttcacc ggacaggctt cgacacaata gccgcaaaaa
     1381 acgcatttcg taatatcgat atagaattca ctggcatagc gctgaagcgg tctggccgta
     1441 tcctcggcac tgatcacttt gatgcaatgt gaagggcagg cgacctcgca gagatcgcag
     1501 cccacgcacc gttcttgacc gtcatcatat cgaagcaggc caagcgcacc gcgatgcgca
     1561 tccgggatcg ttcgttgttc tcgcggatac tgaaacgtca tcggtcgatg aagcatgtgc
     1621 cgaaacgtga ccttcatcgc gtccaagatc tcatagaata acgcggcatg gaggattttc
     1681 ttggttagtg cggcgacact catcgtaggc tcctcgtcat gaacgtagat atccacttac
     1741 gcctgttcaa tacttaccca gatctgcttg aacgatggga caccggtcgt ggcatcaacc
     1801 gacacaggca tcaagtcctt cactggaggt tcattgaaat gttcagggaa aaaacaggtg
     1861 cctggcgcga tgaactgatc gggctgcaca ccgagctgaa gggagcctcg atccgaggtc
     1921 agacgcacct tcgtaccgtc ttgcaaccca agacgctcca tgtcccctat attcatccgc
     1981 agcttcccgg tgttcggagc gatcttgata agcccgggcg cttgggccga caatttcccc
     2041 gaatgcacta ataactgccc catcaacagg gaaaagggac gcgtgcgttc ttccgacgcc
     2101 ggagtctgtt gataacgggc cttgacctcc gccgcgtagc cgttggacaa atactggtca
     2161 gggacgggca cgattttacg gggttgtccg agattatagt acccgggcaa cagcttcatg
     2221 atctcggctt gaatatcgtt ggccgattgg tactcccatt ggcatcccat ggcattcgca
     2281 agggctgtca tgatgtgcca gtccggcagg ctctctccaa gcggatccat tgcctggcga
     2341 atgcgaagca cacgaccttc aaggttcgta aatgtgccgt ccttctcagc ataggtgcaa
     2401 gcggggagca caaaatgagc taattttccc gtatcagtca aaaaaggatc ttgcacgact
     2461 aacagttcga gccgttcgag agcggcacgg acctccatcg aagccggcaa ggtcgcgagc
     2521 ggattttccc ctagaatgta tagggcttta atctgcccac tcttgcaccg tttcaagatt
     2581 tcgatgagat gtgcccctga tccgacaggg gggagggacg tctccc
//
LOCUS       DASTWE010000863         1621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_152355, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000863 DASTWE010000000
VERSION     DASTWE010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152355"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFT30_09010"
     CDS             <1..211
                     /locus_tag="VFT30_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5092812.1"
                     /translation="DRVPTYKFPPLLVNSQRVKPNVTRWYRVRMGDTLEKVSRRFHIP
                     LKTLKSRNHLSSPVIKAGELLVITR"
     gene            complement(268..1047)
                     /locus_tag="VFT30_09015"
     CDS             complement(268..1047)
                     /locus_tag="VFT30_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5092813.1"
                     /translation="MTYRIKVPPRTLPVDEAHLVSGLEHWVLGLANYRWSILVGFLLL
                     LLMGGGIWGVFWYDAQNAGKAQELEREATIHLFTRAANDPQKAAANLKEAIALYKRVV
                     DEYPRTPTAPLAQFSLGNAYLQSNDLAAAIEAYNRFISTYGSHVSLLGLVQQKLGYTY
                     LLKGDLDQAARAYSTILEIPGAMNRDYALFELARLEENRSRPDEAVKHYQDLMKTFPN
                     SPLTSEAAMRVKVIEAKKTPDPSPAAATPAPSSSQSKSSKP"
     gene            1195..>1621
                     /locus_tag="VFT30_09020"
     CDS             1195..>1621
                     /locus_tag="VFT30_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5092814.1"
                     /translation="MFRSKLKELADQSLIRRLSPLESGTGPIIQYAGREVILLSSNDY
                     LGLATHPDVVRAAIHATERYGAGSGASRLVSGTLPPHMHLETSLATFKGTAAALLFGS
                     GYLANIGVIPNLIGQGGLILADRLCHASLIDGCRLSRADF"
BASE COUNT          368 a          455 c          412 g          386 t
ORIGIN      
        1 cgaccgagtc cctacatata agtttcctcc cctgctcgtc aacagtcagc gggtcaaacc
       61 caatgtgact cgctggtatc gagtccgcat gggtgacacg cttgagaagg tgtctcgacg
      121 atttcacatt cctcttaaaa ctcttaaatc gcggaaccat ctgtccagcc cagtgattaa
      181 ggcaggcgag ctcctcgtga tcactcgtta gtacgcgcag gtcttgaacg agtgagaggc
      241 ctcggctctc caaggcaaaa agcttgtcta aggtttggat gacttcgatt ggctggaaga
      301 aggagcggga gttgctgcag cgggcgatgg atctggagtt ttcttagctt cgatcacctt
      361 cacacgcatg gcggcttcgc tcgtcagggg agaatttggg aaggtcttca tcagatcctg
      421 atagtgcttc acggcttcat cgggcctaga gcgattctct tccagcctgg caagttcaaa
      481 gagcgcgtaa tcccggttca tcgcgccggg gatctcgagg atcgtcgagt aggccctggc
      541 cgcttgatcc agatccccct tgagcagata ggtatagccc agtttttgct gtacgagccc
      601 aagaagcgaa acatgagacc catacgtaga gataaacctg ttataggcct caatcgctgc
      661 cgcgaggtcg tttgactgaa ggtaggcatt cccaagactg aattgtgcga gcggggccgt
      721 cggagtccga ggatactcat caaccaccct cttatacagg gcaatcgctt ccttcaggtt
      781 ggcggctgcc ttctgcggat cattggccgc acgtgtaaag agatggatgg tcgcttctcg
      841 ttccagctcc tgagccttgc cggcattctg tgcgtcatac cagaagacac cccagattcc
      901 tccccccatc aaaagaagaa gcaggaaccc gaccaaaatc gaccagcgat agttcgccag
      961 tccaagcacc cagtgctcaa gcccactcac gaggtgagct tcatccaccg gcaacgtccg
     1021 aggtggaact ttaattcggt atgtcatcag atcatcactt tactgttgaa actcagcttt
     1081 ctgcgccaga accgttgctt tatactaagg atgaattcca ttgtcaatgc aatcggaacg
     1141 ccctcgttgt acggttactg cctatctagg ctctaaagaa attttcaaat caacatgttt
     1201 cgaagcaagc tcaaagaact agccgaccaa tcgctcattc gccgcctgtc tccattggaa
     1261 tcaggcacag gccccatcat ccaatatgca ggacgtgagg tcatcttgct gtcctccaac
     1321 gattacctgg gccttgcgac ccatccggac gtcgtccgcg cagccataca tgcaacggaa
     1381 cggtatgggg cggggtcagg agcctctcgc ttggtcagtg gaactcttcc accccacatg
     1441 catctcgaaa cttcccttgc aacttttaaa gggacggcag ccgcccttct gttcgggtcc
     1501 ggttacttgg ccaatatcgg ggtcattccg aacctcatcg gacaaggagg cctgatcctc
     1561 gcggatcgat tgtgtcatgc cagtcttatc gatggatgtc gattgagccg tgccgatttt
     1621 c
//
LOCUS       DASTWE010000864         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_733874, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000864 DASTWE010000000
VERSION     DASTWE010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733874"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFT30_09025"
     CDS             complement(<1..358)
                     /locus_tag="VFT30_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092815.1"
                     /translation="MTVREQLAKAFYDTQSFKWDRGKGFKLASGEISPFYVDCRALMA
                     HPEARRLVAQLAYETITDVEFDCLGGLELGAIPIAVTISDFACVASRRRLWRTFVVRK
                     QPKDHGLGTLIEGSIRP"
     gene            complement(433..966)
                     /locus_tag="VFT30_09030"
     CDS             complement(433..966)
                     /locus_tag="VFT30_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEU5092816.1"
                     /translation="MATQKQGVILVGHGGIPKDCPQDLVTRLKRLEAQRHAAKQPPSA
                     EELELDMKIRRWPRTPSTDPYQSGLQAIAARLQPQLNGILFGLAYNEFCAPTLEEAVE
                     HLIGQGATEIIVATTMLTPGGSHSEIEIPDILDYLRSKHPGVAIRYAWPFDLELVADT
                     LAEQIQRFSTVAPHSDR"
     gene            complement(975..>1855)
                     /locus_tag="VFT30_09035"
     CDS             complement(975..>1855)
                     /locus_tag="VFT30_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEU5092817.1"
                     /translation="KLDHFLTDVLGRYVEWVPVCPEVEAGLGTPREAMRLVGNPHRPR
                     LMTITSKQDHTETMETMIEERLKSLDKLNLSGFVFKKGSPSCGIERVRVYTKQGMQSQ
                     SGSGIFAKAFRHLFPLIPVEDEGRLHDSAQRENFIERVFCYRRFQDLMQEGVTRQALI
                     GFHTTHKYLLLSHSQQHYEAMGRLVGQADRYRLKELTTKYGELFMRTLALKATVRKHV
                     NVLHHIVGYFKTRLKTLEKAELLGVIDDYHRGLTPLIVPLTLIKHYVRLFDVDSIRDQ
                     VYLNPHPKELMLRNHV"
BASE COUNT          411 a          507 c          505 g          432 t
ORIGIN      
        1 caggacgaat gctgccttcg atcaacgttc ccagtccatg gtctttaggc tgcttgcgaa
       61 cgacgaatgt tcgccagaga cgccgacgcg aagcaacgca ggcaaaatcg gaaatggtca
      121 cggcaattgg aatggctccg agttcgaggc cgcccagaca atcgaattca acatctgtga
      181 tcgtctcata ggccagttgg gcgaccagac gacgcgcttc cggatgcgcc ataagggcac
      241 gacaatcgac atagaaaggg ctgatctcgc cggacgcgag cttgaatccc ttgcctcgat
      301 cccatttgaa tgactgagta tcgtagaacg cttttgccag ttgctcccga accgtcacta
      361 taggtcctcc cttttcagag tcagctaggt ggtgggcatt ctacactcac tgatcgactg
      421 ctgacagacc tttcaacgat ccgagtgagg agctaccgtg gagaaccgtt ggatctgctc
      481 ggctaaagtg tcggcgacca actcgagatc aaagggccaa gcataacgga ttgccacacc
      541 agggtgcttc gatcggagat agtcaaggat gtcaggtatc tcgatttccg aatgagaacc
      601 tccaggggta agcatggtgg ttgccacaat gatctcagtg gccccctgtc caatgagatg
      661 ttcaaccgcc tcttccaagg ttggagcaca gaactcgttg taggctagtc cgaacaagat
      721 gccattgagt tgcggctgta atcgtgccgc aatggcttga aggccggatt ggtacggatc
      781 ggtcgatggc gtcctcggcc aacgccggat cttcatatct aattcgagct cttcagcgga
      841 cggcggttgt ttggccgcgt ggcgctgggc ttccagccgc tttagccttg tcaccagatc
      901 ttgtgggcag tccttaggga ttccgccatg tcccaccaga atgactcctt gcttctgtgt
      961 cgccatgaat cctcctacac gtggttgcga agcatcaatt ctttcggatg cggattaagg
     1021 tacacttggt cgcgaatgga gtcgacatcg aacagccgaa cgtaatgctt gatcagcgtc
     1081 agtggaacga tcaagggggt gagccctcga tggtagtcgt cgatcacgcc taacagctcg
     1141 gccttttcga gtgtcttcaa gcgagtcttg aagtagccca caatgtgatg cagcacgttc
     1201 acgtgcttgc ggactgtggc cttcaaggcc aaagttctca tgaagagttc accatacttt
     1261 gtcgttaatt ccttgagtcg ataacgatcc gcttgaccga ccagccggcc catcgcttca
     1321 taatgttgtt ggctgtggga caaaaggaga tacttgtgtg tggtgtggaa gcctatcaga
     1381 gcctgcctgg tgaccccttc ttgcatcaga tcttgaaatc gacggtagca aaacacccgc
     1441 tcgataaagt tctctctttg cgccgagtcg tggagccggc cttcatcctc gacgggaatg
     1501 agaggaaaca gatgtcgaaa agccttcgcg aagatcccgg agccactttg gctctgcatg
     1561 ccctgtttgg tgtacacgcg cacccgctcg atcccgcaac tgggcgaccc cttcttgaag
     1621 acgaaccccg agaggttcag tttgtcgagc gacttgagac gctcctcaat catggtctcc
     1681 atcgtttcgg tgtgatcctg cttgctcgtg atggtcatca gccgagggcg atgcggattg
     1741 cccaccaacc gcatagcttc acgcggggtg cccagtcccg cttcaacctc agggcaaacc
     1801 ggtacccact caacgtagcg gcccagtaca tcggttaaaa aatggtcgag cttgt
//
LOCUS       DASTWE010000865         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1348205, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000865 DASTWE010000000
VERSION     DASTWE010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348205"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFT30_09040"
     CDS             complement(<1..186)
                     /locus_tag="VFT30_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092818.1"
                     /translation="MITARLLAGAAGLGICLGSVTISEAHAAQSIRVLLAPDVQQLEV
                     HTDQTIWVTDEHDQAWSY"
     gene            complement(183..437)
                     /locus_tag="VFT30_09045"
     CDS             complement(183..437)
                     /locus_tag="VFT30_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEU5092819.1"
                     /translation="MPEWTTLGKILIGIGLGIAAIGVLLAVVDRIPGLGNAFSWLGKL
                     PGDISIKRENFSFYFPIATSIVLSVILSLLFYLVGWFFRR"
     gene            complement(494..>1019)
                     /locus_tag="VFT30_09050"
     CDS             complement(494..>1019)
                     /locus_tag="VFT30_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEU5092820.1"
                     /translation="TGAAAMDELMDQTKDLLAFRDVTTKVYPYQIAFNLLPHIGSFNE
                     GGDCSEEVKIAKETRKILGTPGIRVTATTVRVPVLRCHSESINVELERPLKANEARAA
                     LAAMPGVIVYDDPAKKLYPMPLDATGKDEVYVGRVREDESITNGLNLWVVSDNLRKGA
                     ALNAIQIAECLVNG"
BASE COUNT          241 a          304 c          256 g          218 t
ORIGIN      
        1 ataggaccac gcctgatcat gttcgtcagt cacccaaatg gtctgatcgg tatgcacttc
       61 cagctgctgg acatccggag ccaatagcac gcgaatcgat tgcgcggcgt gggcctccga
      121 aatcgtgacc gagccgaggc agatgcccag tccggcagct ccggccaaaa gccgtgcggt
      181 tatcatcggc ggaaaaacca tcctacgaga tagaatagca gactcagaat gacgctgagg
      241 acaatacttg tcgcgatagg gaaatagaag ctgaagttct cacgtttgat ggagatgtca
      301 cccggaagct tgccgagcca actgaaggcg ttccctaatc cgggaatccg gtctacgacg
      361 gccaatagaa cccctatcgc agcaatgccg aggcctattc cgatcaggat tttgccaagg
      421 gtagtccatt ccggcattaa ctttcatccc atgagcggag tacccaacca gccccgtgat
      481 acagcacatg aagctagccg tttactaggc attccgcgat ctgaatcgca ttcagtgcgg
      541 ctcctttacg aagattatcg gaaaccaccc aaagattgag gccattcgtg atcgattcat
      601 cctcccgcac acggccgacg tagacctcat cttttccggt cgcatcgagc ggcatcgggt
      661 acagcttctt cgccggatca tcgtagacaa tcacgccggg catggctgcc agcgcagcac
      721 gcgcctcatt cgctttcaga ggacgctcta attcaacgtt aattgattca gaatgacagc
      781 gcaacacggg cactcgcact gtcgtcgccg tcacccgaat ccccggcgta ccgagtatct
      841 ttcgagtctc tttcgcgatc ttgacttcct ccgaacagtc ccccccctca ttgaacgacc
      901 cgatgtgcgg caacagattg aaggcaattt ggtagggata gactttggtc gtgacgtcgc
      961 gaaacgccag caaatccttt gtttggtcca tcagctcatc catggccgcg gcaccggtc
//
LOCUS       DASTWE010000866         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1445244, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000866 DASTWE010000000
VERSION     DASTWE010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445244"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..705)
                     /locus_tag="VFT30_09055"
     CDS             complement(220..705)
                     /locus_tag="VFT30_09055"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEU5092821.1"
                     /translation="MVIVMFGVTGTGKTRVGSALAESLGWMFLDADDFHEEVNRTKLH
                     RGIPLNDEDRWPWLTRIRGIIQESLTQRRNVVLACSALKRAYRRYLRVGPEVVFVYLR
                     TETHVLEERLKQRRGHFANPVLLQSQLETLEEPLDDTLTVDTTCSPAKIVSLIRDVLK
                     L"
     gene            849..>1275
                     /locus_tag="VFT30_09060"
     CDS             849..>1275
                     /locus_tag="VFT30_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5092822.1"
                     /translation="MAELLVRCLENEGVRYLFTLPGEETLSFMDALADSSIRLITTRH
                     EQGAAFMADVYGRISGKAGVCVSTLGPGATNLATGIGDAFLDRAPLVAITGQASLNRR
                     HKESHQYIDVMSMLKPLTKWNASIPKPEVIPEAVRKAFKI"
BASE COUNT          298 a          350 c          335 g          292 t
ORIGIN      
        1 tcccgccata atcatcgcca gcactcttac aggtctcatg acgagctcat actagtggcc
       61 cagtattatg ccaggtactc gtaaaacccc gaggcgtatg gcgtcacgag caagtgacgc
      121 cacgtttgtg cgcgccggcg agtcggtgag gcagcggtgt cttcgtgagc gggaagtgtc
      181 ttgagggaac gctgctggag gactttttca acatcttgcc tagagtttga ggacatctcg
      241 gatcaggctc acaatcttag ccggactgca cgtggtatcg acggtcagag tgtcatcgag
      301 cggctcttcc aacgtttcta actgactttg gagtaagact ggattcgcga agtggccccg
      361 acgttgtttg aggcgctctt ccagtacatg ggtttcggtc cgcaaataca cgaacaccac
      421 ttcggggccc acacgcaaat atcggcggta cgcacgttta agagctgaac aagccagcac
      481 aacgttccga cgttgcgtca gactctcttg aataattcca cggatgcggg tcagccatgg
      541 ccagcggtct tcatcattca aggggatgcc ccggtgcaac tttgtgcggt tgacctcctc
      601 gtgaaaatca tccgcatcca agaacatcca tcccagcgat tcggctaaag cacttcccac
      661 ccgagtttta cctgtgccgg tcactccgaa catcacaatc accatacaca accctcaaac
      721 caatgcttaa ccctagggag tctctaccga caaatcgaac taggccggcc cctagtcgct
      781 ttcggaatgt ctgaagagta tgtctacgtg gacgtacact tgcagtagag agttcacata
      841 gcatgaatat ggctgaactg ttggttcggt gcctggagaa cgagggagtt cggtacctat
      901 ttaccctccc tggtgaagaa accctcagct tcatggatgc gcttgctgat tcctcaatcc
      961 gactgatcac gaccaggcat gagcaaggag ccgcattcat ggcggatgtc tatgggcgaa
     1021 tcagcggaaa agcaggagtc tgcgtttcca cgcttggacc gggcgctacg aatcttgcga
     1081 ccggtatagg ggatgccttt ctggatcgcg cgcctttggt ggcgattacc ggacaagcgt
     1141 cattgaatcg acgtcacaag gaatctcatc agtacatcga cgtcatgtcg atgctcaaac
     1201 ccctcacgaa gtggaacgcg tctattccca agcccgaggt cattcctgaa gcggttcgaa
     1261 aggcgtttaa gatcg
//
LOCUS       DASTWE010000867         1839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1606951, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000867 DASTWE010000000
VERSION     DASTWE010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606951"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1688
                     /locus_tag="VFT30_09065"
     CDS             54..1688
                     /locus_tag="VFT30_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5092823.1"
                     /translation="MLVFLIHVRDPQFYALPAKTRATNGNVRVMGFPPIGIMSLSSVI
                     KQAGHDCVMFDQADPDTPNHVILDRINREHPALVGLSFLSTTSYPYAKMLAREIRTTN
                     QQVKLAFGGVFASLNASLVKLQCPEVDFVCRGDGEQLILDLMANLENPQDVASLTWMK
                     NGQVIQNPNRAMERQLDQWPFPDRESLKLDYVESMPLDVPVVLSMRRFTTMQTSRGCP
                     WPCIFCDIPIFNEGKWRARSPQHVVAELKYLEELGYESVGFVDDHFLLQPKRIEAICN
                     GIMEAQLSIRWGIEGRVDSVAQHLFPAMAKANCGAMMFGIESGSQKILDRLKKEQTLD
                     QVTTAVKNAKKAGVEIVHGFFTVGNPDETIEDMEATFDFASALPLDTFGFNRLCVYRG
                     TPLWQEYVKRGLVNETRDWYKYFKCSEIDPTCLSGETINRVRQAGIKKLFFYKLIHYP
                     IQTFQLLRRFFRYMPVRDVAYLLLKPFMGQKKGATKAEVVSRLVEHADMKDAAAQLTQ
                     VADEMLHNVFETSRLERVRIQQAAEGTRELPMVQTR"
BASE COUNT          425 a          505 c          508 g          401 t
ORIGIN      
        1 aaggggatgg ttcgcattgt acggcgtgaa ccgttccaga ttgggagaca ggtatgctcg
       61 tttttctcat ccacgttcgc gatccacagt tctacgcgct tccggctaaa acacgcgcca
      121 caaacggcaa tgttcgcgtg atggggtttc ctcctatcgg catcatgtcg ttgtcatccg
      181 tcatcaagca ggcggggcac gactgtgtga tgttcgatca ggccgatccc gacaccccga
      241 accacgtcat cctcgaccgg atcaatcgtg aacatccggc gctcgtcggc ctcagttttc
      301 tcagtaccac cagctatccc tacgccaaaa tgctggcacg cgaaatccga acgaccaatc
      361 agcaggtgaa actcgcgttc ggcggcgtgt tcgccagcct gaatgcctcc ttggtgaagt
      421 tgcaatgtcc cgaagtcgat tttgtgtgtc gcggcgacgg cgagcaattg atactcgatc
      481 tgatggcgaa cttggagaac ccccaagatg tcgcaagcct gacgtggatg aagaatgggc
      541 aggttatcca aaatcccaac cgcgccatgg agcggcaact ggatcagtgg ccctttccgg
      601 atcgcgagag tctgaaactc gactatgtcg agtccatgcc attagatgtg cccgtggttc
      661 tctcgatgag acggttcacg accatgcaaa cctctcgagg gtgcccctgg ccctgtatat
      721 tctgcgacat tccgatcttc aacgaaggaa aatggcgagc ccgtagccca cagcatgtgg
      781 tcgctgaact caagtatctc gaagaactgg gctatgagtc cgtcggtttt gtcgacgatc
      841 actttctgct ccagcccaaa cgaatcgagg caatttgcaa tgggatcatg gaggcacagc
      901 tttcgatccg gtggggcatc gaggggcgtg tggattcagt cgcccaacat ctgtttccgg
      961 ccatggcgaa ggccaattgc ggcgcgatga tgtttgggat cgagagcggc agccagaaga
     1021 ttctcgatcg cttgaagaag gaacagacgc tggaccaggt gacgacggcg gtcaagaatg
     1081 cgaaaaaggc cggcgtggag atcgtgcacg gatttttcac ggtgggcaat ccagacgaga
     1141 cgattgagga catggaggcc accttcgatt tcgcatcagc ccttcccctc gataccttcg
     1201 ggttcaaccg gctctgtgtc tatcggggca cacccttgtg gcaggaatac gtcaaacgcg
     1261 ggttggtcaa cgaaaccagg gattggtaca aatattttaa atgttcagaa atcgatccaa
     1321 cctgtctatc gggcgaaacg atcaaccgtg tacgacaagc cgggattaag aagctctttt
     1381 tctataagct catccattac cccatccaga cctttcagct cctccgccgc ttctttcggt
     1441 atatgcccgt gcgagacgtg gcctatcttc tcctcaagcc cttcatgggc cagaagaaag
     1501 gtgctaccaa agccgaagtg gtctcgcgct tggtcgagca cgccgacatg aaagatgcgg
     1561 cggcgcagtt gacccaagtg gcggatgaaa tgctccacaa tgtgtttgaa acctcgcgtc
     1621 ttgaacgcgt gcgaattcag caagccgccg aagggactcg cgaactgccg atggtccaaa
     1681 ccaggtagga cacacgcagg tcattccccc agagcttctt ggcgcttctg atgctggccc
     1741 actttgggaa ggagggcaag tcggagacag tcatagcaaa aacagtcagg aaacattggt
     1801 cgagatgagc ggcgccacct actcgcgcgt cagtttctt
//
LOCUS       DASTWE010000868         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_669712, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000868 DASTWE010000000
VERSION     DASTWE010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669712"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="pgl"
                     /locus_tag="VFT30_09070"
     CDS             complement(<1..722)
                     /gene="pgl"
                     /locus_tag="VFT30_09070"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU5092824.1"
                     /translation="MPVTPEIHVSRNIQEWVQTAAAFIHAVSEQAIQSTGRCLVALSG
                     GSTPKILYRALRELEWSSRFNWSRIFFLFGDERCTPPEHPESNFGMAQTELFQPLSIH
                     PDHICRMKGEYNPTAAALEYEETLRRVTQCPAPALPRIDLVLLGLGDDGHTASLFPGT
                     AALREQNKIVTVGHAPTGIKSRLTLTLGVLNHAAVVLFLVTGSGKTEMVRRIIESDSE
                     ADRSLPAARIAPESGRLVWMLD"
     gene            complement(724..>1278)
                     /locus_tag="VFT30_09075"
     CDS             complement(724..>1278)
                     /locus_tag="VFT30_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU5092825.1"
                     /translation="ESKDNTNRVLETFQSTGQLPEQANSHPKIAAQDLSSHLQPGAYV
                     SVLAYTTPSRSFETAVNRFRKVLMTQHRVATTFGYGPRYLHSTGQLHKGGPNTGVFLE
                     LVDQMAPDTAIPGKPFSFGTLAKAQATGDMESLSAHHRHAVRVQLGRDQAATVNAITA
                     ALTATSPSRRRRASLKKRPKHSRR"
BASE COUNT          283 a          329 c          371 g          295 t
ORIGIN      
        1 tgatcaagca tccatacgag tcgacccgat tccggtgcta tcctcgccgc cggcaaagaa
       61 cgatctgcct cggagtcaga ctcaatgata cgtctgacca tctccgtttt acctgagccg
      121 gtcaccagga acagtaccac agctgcatga ttcagcacac ctagggtcaa tgtaaggcga
      181 gacttgatgc ctgtgggagc gtgaccaact gtgacaatct tgttctgttc ccgtaaggca
      241 gctgttccag gaaacagcga tgcggtatgt ccgtcgtctc caagacctag aagaacgagg
      301 tcaatgcgag gcagtgccgg agctggacat tgagtaactc ttcgaagcgt ttcctcgtat
      361 tccagagccg cagccgttgg attatactcg cctttcattc ggcaaatatg atcggggtga
      421 atgctgagcg gttgaaacag ctctgtctgc gccatgccaa aattgctctc gggatgttct
      481 ggtggcgtgc accgttcgtc tccaaacaaa aaaaagattc tcgaccaatt gaaacgcgag
      541 ctccactcaa gttcgcgtaa ggcgcggtag agaattttcg gagtcgatcc tcccgaaagt
      601 gcgaccagac accgaccggt ggattgaatc gcctgctcgc tgacggcatg gatgaaggca
      661 gctgccgtct gaacccattc ctgaatgttg cgcgaaacgt ggatttccgg ggtgacgggc
      721 atatcaacgt cgggagtgct taggacgctt cttcaatgac gcacgccgac gacgagatgg
      781 tgaagtcgct gtgagagccg ctgtgatggc attcacggtg gcggcttggt cacggcctaa
      841 ttgaacacga acagcatggc gatggtgggc gctgagcgat tccatgtcac cggttgcctg
      901 tgctttcgcc aatgttccaa acgaaaacgg cttacccgga attgctgtgt ccggcgccat
      961 ctggtccacc agttcaagaa agaccccggt gttggggccc cctttgtgaa gttgaccggt
     1021 tgaatggagg taacgaggcc catacccaaa ggtagtggca acgcgatgtt gtgtcatgag
     1081 gaccttgcgg aagcggttca ccgctgtttc gaacgaacgt gacggagtgg tgtaggccag
     1141 cactgaaaca tatgcgccgg gctgaagatg gctcgacaga tcctgcgcgg caatcttggg
     1201 atggctgttc gcttgttccg gcagttgtcc tgtcgattgg aaagtttcca ggactcgatt
     1261 ggtgttgtcc ttgctttc
//
LOCUS       DASTWE010000869         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1186599, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000869 DASTWE010000000
VERSION     DASTWE010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186599"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFT30_09080"
     CDS             <1..1005
                     /locus_tag="VFT30_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5092826.1"
                     /translation="PRHLESAVKLAARYIEQGREEGDPRFLGQAQAILTPWWNEPIPP
                     VSTLLLRATIRQNAHEFDQALTDLDQVLALQPTNAQAWLTKASILQVQARYDDARRAC
                     QRLTRLVASRVFLGCLGDIAGITGQSAKSLELLRLILLDPGLIGRERIWIATILAETA
                     ARTGTIREAEQYFAEAFKVGIKDQYLLSAYADFLLDQGRYQDVVSLLRSETRTDGLLL
                     RLALAEQALELPAFRNHTAELTARFAAARERGTTAHVREEARFTLALLHDAQRALPLA
                     QANWSVQREPADARILLESALADKNRAAARPVLDWLNINHIEDFRLRQLAKQIQETTF
                     "
     gene            1005..>1586
                     /locus_tag="VFT30_09085"
     CDS             1005..>1586
                     /locus_tag="VFT30_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092827.1"
                     /translation="MRTLLVIAWLLLSLPAWAHKPSDSYLSLSIQNDRVEGQWDIALR
                     DLDNAIGLDRDGSGEITWGEVRNKHDEIVSYALSHLDLSAGGQSCKAQMLEQLIDHHT
                     DGAYSVLRFRSHCGGIIEQLRVDYRLLFEIDAQHKGLLRLTQGGQTSTAIFSRESPAH
                     EFSVTERSRWAESMQFIHEGIWHIWLGFDHVLFL"
BASE COUNT          355 a          470 c          419 g          342 t
ORIGIN      
        1 cctcgtcatc tcgaatcagc cgtgaaactg gccgcacgct acatcgaaca aggccgtgag
       61 gaaggcgatc ctcgtttcct tgggcaagca caagcgatcc ttaccccttg gtggaacgaa
      121 ccgattcctc cggtttccac acttcttttg cgcgcaacca tcagacagaa cgcccatgaa
      181 ttcgaccagg ctttgaccga tctcgatcag gtattggccc tacaaccgac caacgcccag
      241 gcatggctca ccaaagcatc gatcctccag gtacaggcac gttacgacga tgcgcgacga
      301 gcctgccagc ggctcacaag gttggttgcc tctcgcgtgt ttctcggatg tctgggcgat
      361 atcgctggaa taacaggtca atcagcgaaa agcttggagt tactccggct aatactttta
      421 gatccaggcc tcatcggtcg cgagagaatc tggattgcga cgattctcgc cgaaacggct
      481 gcccgcaccg gaaccatccg agaggctgag cagtatttcg ccgaagcctt caaggtcggc
      541 atcaaggatc aatacttgct gagcgcctat gcggatttct tgctggatca aggccgttat
      601 caagacgtcg tctccctact ccgatctgag acgagaaccg atggactgct cttgcgcctg
      661 gctctcgctg aacaggcgtt ggagctgcct gcgttccgaa accatacagc cgaactgaca
      721 gcccgctttg ccgccgctcg ggagcgaggc accacggcac atgtccggga agaagcgcgt
      781 tttacattgg cattgctgca tgatgctcaa cgggcgctgc cgttagccca ggccaattgg
      841 agcgtccagc gagaaccggc ggatgccagg attctcctgg aaagcgcgct cgcagacaag
      901 aaccgagcgg ccgctcggcc ggtgctcgat tggttgaaca tcaaccatat tgaagatttt
      961 cggctgcgac aacttgcgaa acagattcag gaaaccacat tctaatgcgt accctgctcg
     1021 ttatcgcctg gcttctccta agccttcccg cttgggctca caaacccagc gacagctatc
     1081 tctcgctgtc gattcagaat gatcgcgttg aggggcagtg ggatatcgcc cttcgcgacc
     1141 tcgacaatgc gatcggattg gatcgcgatg ggagcggcga aattacctgg ggagaagtcc
     1201 ggaacaagca tgacgaaatc gtttcctacg cgctgtctca cctcgactta tcagctggcg
     1261 gacaatcttg taaggctcaa atgctggagc agctcatcga tcatcacaca gacggagcct
     1321 actcagttct gcgtttccgc tcccactgcg gcggaattat cgaacaactc cgcgtggact
     1381 accgattact gttcgagatc gatgcccaac ataaggggct cttacgtctg acccaaggcg
     1441 gacaaaccag cactgctatt ttcagccggg aatcccctgc ccatgaattt tcggtcaccg
     1501 aacggtcacg ctgggccgag agcatgcaat tcattcacga gggcatctgg catatctggc
     1561 tggggttcga ccatgtcttg ttcctc
//
LOCUS       DASTWE010000870         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_798712, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000870 DASTWE010000000
VERSION     DASTWE010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798712"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2025)
                     /gene="glgP"
                     /locus_tag="VFT30_09090"
     CDS             complement(<1..2025)
                     /gene="glgP"
                     /locus_tag="VFT30_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU5092828.1"
                     /translation="MDSPNVVNSSLPQNLHRLPELAQNLWWSWTLEARQLFELIDPTL
                     WFLTNHNPVKLLSDVKPDRLTRLAEDPSFLRQYSAVFRLFDEYLANKKSWAGTHHADL
                     TKTTIAYFSAEFGLHASVPIYSGGLGILAGDHCKEASDLGLPLVGIGFMYPQGYFRQR
                     ITPEGWQEAAYAPFNRDESPVHLALTPSGEPCRFAVEMGSRRVTAAVWKVLVGRIPLF
                     LIDTDVPENSPEDRALSARLYGGDQEMRLCQEILLGIGGVRMLRSLGISPSVWHANEG
                     HSAFLTLERVREFVQKGSSHAEASESVRQSTVFTTHTPVPAGHDVFPHYLMDRYFSGY
                     WEQLGLSREEFLHLGETPESREYGFNMTALVMRLSAHVNGVSREHGRVTREMWQHFWP
                     GLPADQVPIRSVTNGIHAPTWISPELNHLYSKSLSPTWTRTCDDPAMWQRIMDVPDHE
                     LWAVRQTIKRKLMGFIRERARAAWMHGHLQPSQVLTRGTLLDPEALTIGFARRFATYK
                     RATLLFRDLERLKALLQDRWRPVQLIFAGKAHPADEPGRFFIHEVLNFCHDHKLGGRI
                     AFLEDYEMHMAKYLVQGVDIWLNTPRFPMEASGTSGMKAALNGVLNLSVLDGWWVEGY
                     NGANGWGIQPLNEQADAQAQDHHDAEQLYRVLEQEVVPLFYQRDRDGIPRG"
     gene            complement(2089..>2953)
                     /locus_tag="VFT30_09095"
     CDS             complement(2089..>2953)
                     /locus_tag="VFT30_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092829.1"
                     /translation="IIRTLLREPMATTTAEYKERLRQLAELMLAVSGESVTMIKRNVP
                     EQALKLKRQKEWGVYLEFLKIMFNLTDRLVALHIPMKDQMEFMNGLEDAVTQQLKRAL
                     EPAFGNNADQMEIVLTIGTTVADSRQTYEKYKFLISEDSKMKNEMFREFGEKVADALG
                     AAGNAQLSSAATLCASAVVPALSSILQGQAPPSQEASQTAPPTVGTSTEQEKTTGQEI
                     KLVSLMSSVSGEEVETRWGLHPRFREDLTPSELQQLTKLLNRVAKILGERYAAVAFSK
                     DWASWHKAGHA"
BASE COUNT          608 a          834 c          859 g          652 t
ORIGIN      
        1 gccgcgggga atgccgtcac gatcgcgctg atagaacagc ggcacgactt cttgttccaa
       61 tacgcgatag agctgctcgg catcatgatg atcttgggcc tgcgcgtcgg cctgttcgtt
      121 caggggttga attccccatc cgttggctcc gttatagcct tcgacccacc atccatcgag
      181 gacgctgagg ttcaacacgc cgttcagggc ggctttcatg ccgctggtgc cgctggcttc
      241 catcggaaaa cgaggagtgt tcaaccaaat gtcgacgcct tggacgagat atttcgccat
      301 atgcatttcg taatcttcaa gaaaggcgat gcggccgccc agcttatggt catggcagaa
      361 gttcagcacc tcgtgaatga agaatcgacc aggctcatcg gccggatggg ccttgccggc
      421 gaagataagt tggaccggcc gccatctgtc ttggaggagt gccttcagtc gttccaggtc
      481 tcggaagagc aaggtggccc gtttgtacgt cgcgaacctt cgagcgaatc caatggtcaa
      541 agcttcgggg tccaggagtg tgccgcgtgt gaggacttga gacggctgca ggtggccgtg
      601 catccaggcg gctcttgctc gttcgcggat aaaacccatg agtttccgct ttatcgtttg
      661 tcgaaccgcc cacagctcat ggtcggggac atccatgatc cgttgccaca tggccggatc
      721 atcgcaggtg cgggtccagg tcgggctgag cgacttgctg tacagatggt tcagttccgg
      781 cgaaatccaa gtcggcgcat gaatgccgtt cgtcacgctg cggatgggga cctggtcggc
      841 cggtaaccca ggccagaagt gctgccacat ctcgcgagtc acgcggccat gttcgcggct
      901 gacgccgttg acgtgggccg acagtcgcat gaccagcgca gtcatgttga aaccgtattc
      961 tcgcgattcg ggagtttctc ccagatggag aaattcctcg cgggagaggc ccagttgctc
     1021 ccagtagccg gaaaaatatc gatccatcag atagtgcgga aagacatcgt gcccggccgg
     1081 cacgggtgtg tgcgtcgtga agacggtgct ttgacgaacc gactcgctgg cttccgcatg
     1141 ggacgaaccc ttttgaacaa actcgcggac ccgctccaag gtgaggaagg ccgaatgacc
     1201 ttcattggca tgccagaccg atggcgagat gccgagcgag cgcaacatgc gtacgccgcc
     1261 gatgcccagc aaaatttcct gacagagccg catttcttga tccccgccat agagacgtgc
     1321 ggagagggca cggtcctccg gactgttttc cggcacatcc gtatcgatga gaaagagcgg
     1381 aatccttccc accagcactt tccagacagc cgcggtcaca cggcggctgc ccatctcaac
     1441 ggcaaaccgg cagggctctc ccgacggagt gagggccaga tgaaccggag attcatcgcg
     1501 gttgaaaggg gcataggccg cctcttgcca accttccggc gtgatccgct ggcggaaata
     1561 cccttgcggg tacatgaatc cgatgccgac gagtggcaag ccgaggtcac tcgcctcttt
     1621 acaatggtcc ccggccaaga ttccgagacc accgctgtaa ataggtacgg aggcatgcaa
     1681 cccaaattct gccgagaagt atgcaatggt ggtcttcgtc aaatcggcat ggtgcgttcc
     1741 ggcccaactc ttcttattgg ctaggtactc atcgaatagg cggaacaccg ccgagtattg
     1801 gcggagaaat gacgggtctt cggccaaacg cgtcaaccgg tccggtttca catcggacaa
     1861 gagtttgacc ggattgtggt tcgtgagaaa ccacagcgtc ggatcgatca gttcgaacaa
     1921 ctggcgagct tccaacgtcc aactccacca gagattttgt gccagttccg gcagacgatg
     1981 gagattctgc gggagagaac tattcaccac attaggagaa tccacaaaca ctcctttggt
     2041 caagatcgca taatcggtat cggcaatgac tggcaatcac ggctgggatc aggcgtgccc
     2101 tgccttatgc cacgaagccc agtctttgga gaatgccacg gcggcatacc gttcgccaag
     2161 gatttttgcg actcggttta ggagtttggt gagttgctgg agttcggagg gggtgaggtc
     2221 ctcgcgaaac cgtggatgga gtccccaacg ggtctcgacc tcttcacccg atacactcga
     2281 catgaggctc acgagtttga tctcttggcc ggtcgttttt tcctgttcgg ttgaggtccc
     2341 aacggtggga ggcgcagtct gtgatgcttc ctgagaggga ggagcctgtc cttgcagtat
     2401 tgagctgagc gccggcacga ccgcactggc gcaaagcgtt gccgctgagc taagttgcgc
     2461 gttgccggct gcgccgaggg cgtcagctac tttctcgcca aactcgcgga acatctcatt
     2521 cttcatcttg gagtcctccg agatcaggaa tttatatttc tcgtaggtct gccgactatc
     2581 cgccaccgtc gtgccgatcg tcagcacgat ttccatctga tccgcgttgt tcccaaacgc
     2641 cggctccagc gcgcgcttca attgttgcgt cacggcgtct tccagcccgt tcatgaactc
     2701 catctggtcc ttcatcggga tgtgcagggc caccagccgg tccgtgagat tgaacatgat
     2761 cttgagaaat tccaaataga cgccccactc cttctgacgc ttgagcttca atgcctgctc
     2821 gggaacattg cgctttatca tcgtgaccga ttcgcccgac acggcgagca tcagttcagc
     2881 cagctggcga aggcgttcct tgtattcagc ggtggtagtg gccattggtt ctcttagtaa
     2941 tgtgcggatt ata
//
LOCUS       DASTWE010000871         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_346498, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000871 DASTWE010000000
VERSION     DASTWE010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346498"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..>2034
                     /locus_tag="VFT30_09100"
     CDS             76..>2034
                     /locus_tag="VFT30_09100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEU5092830.1"
                     /translation="MGVSDFGTEITPESVADSLVARAAPAIEAPPASIESLESVDVDV
                     LTQMDPRLQRAVVRARQGIVKLPGASTETDEVAVVATVTDPDAWESLSEVRMGAVIGT
                     DESGETMVTGRIPVRRVEAVRHQPFVTSLKASQRLQPSLDRTIAEIGARGDLLPNGNQ
                     GDGGRGAIVGIIDFGCDFAHENFRNADGTTRLLAIWDQRGPSSPSSPFGFGKVHTRND
                     INAALNQPNPYAALGYGPPPDNAFQQGSHGTHVMDIAAGNGRGSGISGVAPNADIIFV
                     EAAANDIPFSGPNAVGKSFGDSVQLLEAVKFIFEQAGNRPCVINASLGTNGGPHDGTT
                     LVENGIDRLLRQAPNRAMVIAASNSFSDGIHATGRVTPGGTVDLVWNVPSQDSSSNEL
                     EIWYDGADRFGVDVIAPNGETVISVEPDQTRALNSNGRVVLLAANRLADPNNQDNMIG
                     IFLEAGLPLGRWTIRLRGLTVQDGSFHAWIERDDAGQSSFPEPLDNSHTIGSISCGRE
                     TIVVGSYDAHKPTLPLSFFSSAGPTRDGREKPEVSGPGHAVFAAHSRTRTGTIRKSGT
                     SMASPAVAGVVALLLSEASARGQSMTSAQIRNSVITTARRTPPPGAQWDPRFGFGRVS
                     AHDAVSGVITASPVTSISSNGGSNGANNG"
BASE COUNT          475 a          542 c          580 g          437 t
ORIGIN      
        1 atggcccagg tagtttgccc cagctgtcgc gctgttgtcg cggccctccc attttgctca
       61 cgttgtcgtt caccgttggg tgtatctgat ttcggaaccg aaattacgcc ggagtcggtc
      121 gcagatagcc tggtcgcgcg cgcggcccca gccatcgaag cgccaccagc ctcaatcgaa
      181 tcacttgaat cagtggacgt agacgtgctg acgcaaatgg atccacgtct gcaacgcgct
      241 gtagtgagag cacgtcaggg aatcgttaaa ttgccaggag catccactga aacggatgag
      301 gtagccgtag tcgctacagt gaccgatcca gacgcctggg agtcactgag cgaggtgaga
      361 atgggagctg tcataggaac tgacgaaagc ggagaaacga tggtaacggg aagaattccc
      421 gtccgcaggg tggaagctgt tcgtcaccag cctttcgtca ccagtttgaa agcctctcaa
      481 agattacaac cctccctgga tcgaactatt gcagagatcg gagctcgcgg agatctattg
      541 ccaaacggca atcagggcga tggcggtcgc ggtgcaatcg tcgggatcat cgactttggc
      601 tgcgacttcg ctcacgaaaa tttccgcaac gcggatggca ccaccagact tctcgcgatc
      661 tgggatcaac gtggtccatc aagcccttca agtccgtttg gtttcggaaa agtgcatacc
      721 cgaaacgata tcaacgctgc acttaatcaa cccaaccctt atgccgcgct tggctatggt
      781 ccacctccgg ataacgcgtt tcagcaaggt agtcatggta cgcacgtaat ggatatcgct
      841 gcgggaaatg ggcgcggatc tggtatatcc ggagtggcgc ccaatgcgga cattatcttt
      901 gtcgaagctg ctgctaacga cattccattc tcaggaccca acgcagtcgg aaaatcgttc
      961 ggcgattcag tccagctttt ggaagccgtg aagtttatct tcgagcaagc gggaaatcgt
     1021 ccatgcgtta tcaatgccag cctcggtaca aacggaggac cacacgacgg cacaaccctc
     1081 gtcgagaatg gtatcgaccg gctgttgagg caagcgccga acagggcgat ggtgatagcc
     1141 gcttcgaatt cctttagcga cggcatacac gcaaccggaa gagtgacgcc gggtggaacc
     1201 gttgacctgg tttggaatgt tccttcacag gattcgtcca gtaatgaatt ggagatctgg
     1261 tatgacggag cagatcgctt tggagttgat gtcatagctc caaacggcga aaccgtgatc
     1321 agcgttgagc ccgatcaaac tcgtgctttg aatagtaacg gacgagtggt gttgctggcg
     1381 gcaaatcgtt tggcggatcc taacaatcag gacaacatga ttggtatttt cctggaagcc
     1441 ggccttcctt taggccggtg gactattcgc ttgcgaggac tcacagtcca ggacggcagt
     1501 ttccacgcct ggatcgaaag ggacgacgcc ggccaatcga gttttccgga gcctctggac
     1561 aacagccata ccatcggctc gatctcctgt ggccgggaaa caatcgtggt tggctcgtat
     1621 gacgcgcaca aacccacctt gccgttgtcg ttcttttcga gtgctggtcc aacacgcgat
     1681 ggtcgcgaaa agccggaagt ttcggggccc ggtcatgctg tctttgcagc tcactcaaga
     1741 acaaggaccg gaacaatacg caagtcaggg acaagcatgg cctcgccggc agtagctggt
     1801 gtcgtggcat tgctattatc cgaagcgagc gcgagaggac agtctatgac gtcggcgcag
     1861 atcagaaact ccgtgatcac taccgctcgt cgcactcctc cgccgggagc gcagtgggat
     1921 ccaaggtttg gatttggaag agtttcagct catgacgcag tgagcggcgt tataacagcc
     1981 tcacccgtta cgagcatctc atccaacggt ggaagcaacg gcgcgaacaa tggt
//
LOCUS       DASTWE010000872         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_896216, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000872 DASTWE010000000
VERSION     DASTWE010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896216"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="VFT30_09105"
     CDS             complement(<1..1361)
                     /locus_tag="VFT30_09105"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092831.1"
                     /translation="MSEANTPTPSWLAPLRSALGREEARLCLLGAHGSTAACALTLLN
                     DTRQGIPDRARPWVVVTSNDESAERMFNDLTFFYELTGRPVEDLAWFPEWETLPYEAT
                     APHVGLIAHRMTTLHRLLVSTPTILVTSVAAAMHRVLPRSTFEQAVFRFETAAAFERD
                     LLITDLLRLGYRRVSVVEIPGEFSIRGGIVDIFSTAYPNPIRVEFLGDQVESIRLFDP
                     STQTSITKLNEGWVLPAREFVRSSDDPDAIVPIQADAEWRSPDLYESMDTLFDYLSTS
                     PYLALDQPDALKKACESAWEKIDDGYLRHVDRDATHPYPSPERLFLTWQGIQQRIERW
                     PMLALEPLAAPDSTWNQTFSFSAQAPGSIGLGIRGTAFSQTLSLLEGLRNEHRVVLVA
                     RSRGQVDRLLALLREHDLPADPWKPMVWSSHTTGKPPFYVLHGDLSAGFLSGDLRLAL
                     LTE"
     gene            complement(1365..1847)
                     /gene="rfaE2"
                     /locus_tag="VFT30_09110"
     CDS             complement(1365..1847)
                     /gene="rfaE2"
                     /locus_tag="VFT30_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEU5092832.1"
                     /translation="MTTKVLPRDQLLSLLSSERAKGKRIVFTNGCFDLMHIGHTRYLQ
                     AARALGDILVVGVNSDASVHTLEKAPDRPIVPEAQRAEILAALGCVDFVVIFNESDPL
                     QLITAVQPDILVKGGDWGIDRIVGREIVEARGGVVKTIPLVPGLSTTTLIQRIRSTVK
                     "
     gene            2026..>2260
                     /locus_tag="VFT30_09115"
     CDS             2026..>2260
                     /locus_tag="VFT30_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092833.1"
                     /translation="MLGAHEMVGAPQRSHSNKPILSRPPIYARQLALIVLISVVTLVA
                     GCFGFMTLKHKLIAASGEMIALMATEVATQLDVL"
BASE COUNT          493 a          631 c          679 g          457 t
ORIGIN      
        1 tcttccgtca agagcgcgag ccgaaggtct cccgaaagaa acccggccga tagatcgcca
       61 tgcagaacat aaaatggcgg cttaccggtt gtgtgactgg accacaccat cggcttccat
      121 gggtcggcag gcaaatcgtg ctctcggagt agagcgagca agcggtcgac ctgcccgcgg
      181 cttcgcgcca ccaagacgac ccgatgttcg ttccgcagtc cttccaacag gctcaatgtt
      241 tggctgaagg cggtgcccct gatcccgagt ccgatacttc cgggcgcttg ggcggaaaac
      301 gaaaacgtct gattccaggt tgagtccggc gccgccagcg gctcaagagc caacatgggc
      361 catctctcga tacgttgttg gatgccttgc caggtcagga agaggcgttc gggcgaggga
      421 tacgggtgtg tggcgtcacg atccacatgg cggagatatc cgtcgtcgat cttctcccag
      481 gccgactcac aggctttttt cagcgcatcc ggttgatcga gggcgaggta tggagatgtg
      541 ctgagatagt cgaacagggt atccatggac tcgtacaggt cggggcttcg ccactcagca
      601 tccgcttgga tcggcacgat tgcatcgggg tcgtctgaag accgaacgaa ctccctggcg
      661 ggcaacaccc agccctcgtt caattttgtg atggacgtct gggttgaggg gtcgaacaag
      721 cgaatcgatt cgacctggtc cccaagaaat tctacacgga tcgggttggg gtaggcggtc
      781 gaaaatatat caacgatgcc gccacggatg ctgaactccc ctggaatttc cacgacggac
      841 actcgtcgat accctaggcg gagtaggtcg gtgataagca agtcccgttc gaaggctgcc
      901 gctgtttcga agcgaaagac cgcctgctcg aatgtcgagc gcggaaggac tcggtgcatc
      961 gcagcggcga cggaggtgac gagaatggtg ggcgtgctga ccaacaggcg atggagcgtc
     1021 gtcatccggt gcgcaatcaa tccgacatgc ggcgccgtcg cttcataggg gagcgtttcc
     1081 cattccggga accaggcgag atcttcgacc gggcgacccg tgagttcata gaagaaggtc
     1141 aagtcattga acattcgttc ggcggattcg tcgttcgaag tcacaacaac ccatggacgg
     1201 gcgcggtctg gaataccttg gcgggtgtcg ttcagcaggg tcagcgcgca ggccgcagtc
     1261 gagccatggg ctcccagcag gcagagtcga gcttcttccc ggccaagtgc cgaacgaagc
     1321 ggagcgagcc aggatggggt gggggtgttg gcctcagaca cgcatcactt cacggttgac
     1381 cggatgcgtt gaatgagagt ggttgtggag agaccgggaa ccagcgggat tgtcttgacc
     1441 acccctccac gagcttcgac aatctcgcgg ccgacgatcc gatcgatccc ccaatccccg
     1501 cccttgacca gaatgtccgg ttgaacggct gtgatgagct gaagggggtc cgattcgtta
     1561 aaaatgacca caaaatctac acagcctaaa gctgccagta tttctgcccg ttgcgcctca
     1621 ggcacaatcg gtcggtcggg tgccttctcc agcgtatgga ccgaggcgtc actgttgaca
     1681 ccgacgacca ggatatcgcc gagggccctg gccgcctgca gataccgggt atgtccgatg
     1741 tgcatcaggt cgaaacagcc gtttgtaaaa acgatccgtt tcccttttgc ccgttcactg
     1801 gaaagtaagg agaggagttg atcgcggggc aacaccttcg tggtcataac cccatcatac
     1861 ctggcaagcc ctttccaggg aaagagcacg gaaaggcata tacatgagcc ggtccatggg
     1921 aggccggaca tattccagtc tcagcctcaa gtcgtcacct aacggaccga tacagtacgt
     1981 aacgggatct aatgcctagc gtcagacctg cttgagtcta ctgatatgct cggagcacat
     2041 gaaatggtag gagcgccgca acggtcccat tcgaataagc cgatcctctc gcggccaccc
     2101 atctatgccc gccagctggc cttgattgta ttgatcagtg tggtgacgct cgtggctggg
     2161 tgcttcggtt ttatgaccct caaacacaag ctcatcgcgg catcaggtga aatgatcgct
     2221 ttaatggcga cggaagttgc cactcaactc gacgtcctct
//
LOCUS       DASTWE010000873         1118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_573005, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000873 DASTWE010000000
VERSION     DASTWE010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573005"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="ltrA"
                     /locus_tag="VFT30_09120"
     CDS             complement(<1..1080)
                     /gene="ltrA"
                     /locus_tag="VFT30_09120"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU5092834.1"
                     /translation="MTKAPISLQDLRRRLYIKAKTEPSWRFWGLYVHVCKRETLQQAY
                     LSAKANDGAPGIDGVTFAAVEAGGVQEFLDQLRKELVERTYRPQAARKVEIPKDGGKM
                     RQLSIPSIRDRVVQGALKLILEPIFEADFQPGSFGYRPNKSAHAAIQRVSKAFLEGKT
                     YVIDFDLRSYFDTVRHSIVLAKVARRIDDDAVLWLLKLLLDTSGKQGVPQGGVISPLI
                     SNLYLNEVDQMLERAKAVTQHERWTAVEYARFADDLVILVDSHPRQQRLQQAVEKRLR
                     EELAKLQVEVNEEKSRTVDLQRGESFGFLGFEFRRIRSRRGRWMPLLLPKGKKRTALL
                     GKLKEIFRASRSQPVGSVIEKINPIL"
BASE COUNT          234 a          367 c          259 g          258 t
ORIGIN      
        1 cagaatcgga ttgatctttt cgatcacact gcccaccggc tgggaacgag aggcccggaa
       61 aatctctttc agtttgccaa gcaatgccgt tcgcttcttg cccttcggca ataacagcgg
      121 catccatcgt ccccggcgac tccggatacg acggaactcg aagccgagaa acccgaagct
      181 ctcgcctcgc tgaaggtcca ccgtgcggct cttctcctca ttcacctcca cctgcagttt
      241 ggccaattcc tcccgcagcc gtttctccac cgcttgctga agcctttgct ggcgcggatg
      301 cgaatccacc agaatcacca gatcatcggc gaacctggca tactcgactg ccgtccaccg
      361 ctcgtgctgg gtcactgctt tcgcacgctc cagcatctgg tctacctcat tgaggtacag
      421 attactgatc agcggcgaaa tcaccccgcc ttgcggaacg ccctgctttc ctgacgtatc
      481 cagcaacaac ttcaagagcc acaaaaccgc atcatcatcg atccgccgcg ctaccttcgc
      541 cagcacgatg gaatgtcgca ccgtgtcgaa gtaagatcgc agatcaaaat cgatgacgta
      601 ggtcttaccc tccagaaatg ccttcgatac gcgctggatg gcagcgtgcg ctgacttatt
      661 cggccggtag ccgaacgacc ccggttgaaa gtcggcctcg aagatcggtt ccaggatcag
      721 ctttagcgct ccctggacca cccggtcccg aatggatgga atcgaaagct gacgcatctt
      781 gccgccatcc ttcgggattt ccaccttgcg tgcggcctgt ggccgatatg tccgttccac
      841 caactccttg cgaagttggt caagaaactc ctgtacgcct cctgcttcca ctgccgcaaa
      901 cgtcacaccg tcaattcccg gtgctccgtc attagctttg gctgacaagt aggcctgctg
      961 aagtgtctcc cgcttacaaa cgtgaacata caatccccaa aagcgccaac tcggttcggt
     1021 cttcgccttg atgtatagtc ttcgtctcag gtcctgcaga ctaataggtg cctttgtcat
     1081 ctcacctctg cctcccttct tgataaagac tgcatgtg
//
LOCUS       DASTWE010000874         1218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_443479, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000874 DASTWE010000000
VERSION     DASTWE010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443479"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFT30_09125"
     CDS             <1..325
                     /locus_tag="VFT30_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092835.1"
                     /translation="QCGPLGLNECGGASFRDIAGQGPESPPDLWGEERKMLRAYLNYG
                     LIASVVVWAAIVGMMAYRLNESPWRWAFVGLVFAGGLTVTAILWIKKYVDRLNKTEEK
                     QESKS"
     gene            322..561
                     /gene="purS"
                     /locus_tag="VFT30_09130"
     CDS             322..561
                     /gene="purS"
                     /locus_tag="VFT30_09130"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU5092836.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEL
                     DLQQTDKAKAEVEVKQMCEKLLANTIIEEYRYELG"
     gene            643..>1218
                     /gene="purQ"
                     /locus_tag="VFT30_09135"
     CDS             643..>1218
                     /gene="purQ"
                     /locus_tag="VFT30_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5092837.1"
                     /translation="MNIGVVVFPGSNCDHDCRHVFRDVLGQDVTMVWHKETSLAGLDA
                     VILPGGFSYGDYLRTGAIARFSPVMQVVKQFAADGGLVLGICNGFQILLEAGLLPGAM
                     LRNKSLHFICRETCVKVENAATPFTIACRSGQVLKIPIAHADGNYYTDPVTLAGLQAN
                     AQIVFRYCTTDGKVTSDACPNGSLDNIAGIRN"
BASE COUNT          274 a          269 c          379 g          296 t
ORIGIN      
        1 gcaatgcgga ccgctcgggc tcaacgagtg cggtggcgcg agtttccgtg atatcgcggg
       61 acaaggccca gaatctcctc ctgatctttg gggggaagaa agaaagatgc tgcgggcgta
      121 tttgaattat ggcctgatcg catcggtggt cgtttgggcg gcaatcgttg ggatgatggc
      181 ctatcggttg aacgagtcgc cctggcgctg ggcgttcgtt ggacttgtgt ttgccggtgg
      241 attgaccgtc acggccatat tgtggatcaa gaaatacgtg gatcggttga acaaaaccga
      301 agagaagcag gagagcaagt cgtgaaagcc aaaattcatg tgacgttgaa gcaaggcatc
      361 ttggatccgc aaggcaaagc catcgaacat gcgctggact cattggggtt taagaatgcg
      421 gcgaatgtgc gtgttgggaa atacatggag ctggatcttc agcagacaga taaggcgaag
      481 gccgaggtcg aggtgaagca gatgtgtgaa aagcttctgg ccaatacgat catcgaagag
      541 taccgctacg aacttgggta ggagcgcgat agcgtatggc ttatggcaca agctttggat
      601 cgtccgatct cgtgctatac gccataagct cctcaaaaaa ttatgaacat cggtgtcgtt
      661 gttttccctg gtagtaattg cgatcacgat tgtcggcacg tcttccgtga cgtgctgggg
      721 caagacgtga cgatggtgtg gcataaggaa acttccttgg ccggtttgga tgcggtgatc
      781 ctgcctggtg gattttcgta tggtgattat ctccggactg gcgcgattgc gcggttttcc
      841 ccggtcatgc aagtagtcaa gcagtttgcc gccgatggag gattggtgct cgggatctgc
      901 aatggctttc agattttgct ggaagcgggt ttgttgccag gcgccatgtt gcgaaataag
      961 tcgttgcatt ttatctgtcg cgagacctgc gtcaaagtgg agaacgctgc gacgccgttt
     1021 acgattgcct gcaggtcggg acaggtgctc aaaatcccga tcgctcacgc ggatgggaat
     1081 tactacaccg atccagtaac attagccggt cttcaagcca atgcacagat cgtcttccgt
     1141 tattgcacga cggatggcaa ggtgacgtcg gatgcttgtc cgaacggctc gctcgataat
     1201 attgcgggca tccgcaac
//
LOCUS       DASTWE010000875         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1283525, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000875 DASTWE010000000
VERSION     DASTWE010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283525"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /gene="lon"
                     /locus_tag="VFT30_09140"
     CDS             complement(<1..1543)
                     /gene="lon"
                     /locus_tag="VFT30_09140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5092838.1"
                     /translation="MKEQIERIVSLGKVLIPDVMVVIENLEEPGRLADMVVSNLGLKV
                     DATQAVLEISDPIQRLRQVSDILGKEIEVLSMQQKIQAQAKGEMDKTQREYFLREQLK
                     AIQKELGELDERAEEITEFRKRIKDSKMPDKVLKETEKQLKRLEKMHPDTAESATVRT
                     YLEWMVELPWSKRSKDNLELKAAAKVLNDDHYDLEKVKERILEYLAVRKLKEKMKGPI
                     LCFVGPPGVGKTSLGKSIARALGREFVRISLGGVRDEAEIRGHRRTYVGALPGRIIQG
                     MKQAGTGNPVFMLDEVDKVGMDFRGDPSAALLEVLDPEQNSAFTDHYLGVPFDLTEVM
                     FITTANLIDPILPALRDRMEVIEIPGYTEEEKLGIAQKYLIPRQLEEHGITNKHVRLT
                     EPAIRQIISHYTREAGVRNLEREIANVMRKVAKKVAEGKGQGFPIDPSNLHKYLGVSK
                     YVPEAELEKDEIGVATGLAWTEAGGDVLYIEATVMKGKGQLTLTGHLGDVMKESAQAA
                     LSYVRS"
BASE COUNT          294 a          454 c          396 g          414 t
ORIGIN      
        1 gtgagcggac gtagctaagc gcggcctgag cggattcttt catcacatca cccagatgtc
       61 cggtcagggt taattgacct ttacccttca tgaccgtcgc ttcgatatag agcacatcgc
      121 cgcccgcttc tgtccaggcc aggccagtcg ccacgccgat ttcatctttc tcaagttccg
      181 cttcgggcac atatttggac acgcctaaat atttgtgaag attggatgga tcaatgggaa
      241 acccctggcc tttgccctca gcgactttct ttgcgacctt acgcatcaca ttggcgatct
      301 cgcgctcaag gttccgcacc ccggcctctc gcgtgtagtg cgaaataatt tggcggatcg
      361 ccggttccgt aagacggaca tgtttgttcg taattccgtg ctcttcgagc tggcggggaa
      421 tcaggtattt ttgggcgatg ccgagtttct cttcttcggt atacccggga atctcgatga
      481 cctccatacg atcgcgtaac gcaggaagaa tcggatcgat caaattcgcc gtggtgatga
      541 acatcacttc ggttaggtcg aatgggaccc cgagataatg gtcggtgaat gcgctgtttt
      601 gttccgggtc gaggacctcc agcaaggctg ccgaggggtc gccccgaaaa tccatcccga
      661 ctttgtccac ctcatcgagc atgaacacgg ggtttccagt tcccgcttgc ttcatgccct
      721 ggataatgcg tcctggtaac gcgccgacat aggtgcggcg atggccgcgg atttcagcct
      781 catcccgcac gccgcccaag ctgatacgta caaactcacg ccccaatgcg cgggcgatcg
      841 acttccccag tgatgttttt ccgactcccg gtgggccgac gaaacagagg atcggcccct
      901 tcatcttctc cttcaacttg cggaccgcca ggtattcgag aatccgctcc ttaacctttt
      961 cgaggtcata atggtcgtcg ttcaacacct tggcggcggc tttcagctcg agattgtcct
     1021 ttgatcgctt ggaccatggc aattcgacca tccattccag atatgtccgc accgtcgcgg
     1081 attcggcggt atccgggtgc atcttttcta gacgcttgag ctgtttttcg gtctccttca
     1141 agaccttgtc gggcatcttc gaatctttga tccgtttgcg gaattccgtg atttcttccg
     1201 ctcgctcatc gagttcaccc aactcctttt gaatggcctt cagttgttcc cgcagaaaat
     1261 attcccgttg ggtcttgtcc atctcgccct tggcttgcgc ctggatcttt tgctgcatgg
     1321 agaggacttc gatttccttc ccgagaatgt cgctcacttg gcgaaggcgt tgaatcggat
     1381 cggaaatttc caagacggcc tgagtggcgt cgaccttgag accgagatta gagaccacca
     1441 tgtcggccaa tcggcccggc tcttccaggt tttcgatgac caccatgaca tcggggatca
     1501 agacttttcc caggctcacg attcgttcga tctgctcttt cacggttctc atgacggc
//
LOCUS       DASTWE010000876         1472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_896315, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000876 DASTWE010000000
VERSION     DASTWE010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896315"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFT30_09145"
     CDS             complement(<1..221)
                     /locus_tag="VFT30_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092839.1"
                     /translation="MSELVCIAFKDLSTADRVLNELRAKETEYVLDLEDAVIVVRDMD
                     GKVHLKQCVDVFSGATTHGVALGVLWGGL"
     gene            434..1054
                     /gene="ccmA"
                     /locus_tag="VFT30_09150"
     CDS             434..1054
                     /gene="ccmA"
                     /locus_tag="VFT30_09150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEU5092840.1"
                     /translation="MLQAVSLHCSRGERRLFSDLNVKVERGSLLAVVGENGSGKTSLL
                     RIFSSLLPPEQGSVLWEGQDIHQLKELYSGQLTYIGHLNGIKDDLTPLENLMSAMALA
                     GEPCSHSGAKEALEAIGLKRPIHHLPSKVLSQGQKRRVALAKLWLSTRPLWLLDEPFT
                     SLDTASTSIVTQRLLTHLQRGGLAVVVTHQEVALPAETVQRLRLTG"
     gene            1051..>1472
                     /locus_tag="VFT30_09155"
     CDS             1051..>1472
                     /locus_tag="VFT30_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU5092841.1"
                     /translation="MIGSSMWEAVSGIVRRDLLLAMRRRSDAAMSVFFLVIVVSLFPL
                     GVGPEPAVLRTIGPGVLWVAALLACLLSLGRVFTADYLDGVLEQMVLIPQPLAVLVVG
                     KVLAHWLISGLPVVLLSPLLGLQFGLSGESLGVLVMSL"
BASE COUNT          282 a          398 c          398 g          394 t
ORIGIN      
        1 atcagccctc cccataacac gcccagtgca actccatgcg tagtcgctcc gctgaacaca
       61 tcgacgcact gtttgagatg cactttcccg tccatatcgc ggacgacaat gaccgcgtct
      121 tccagatcca ggacatactc tgtttccttg gctcgcaatt cgttcagcac ccgatcggct
      181 gtgcttaaat ccttaaacgc gatgcagacg agttcactca tgattcatct ccttcaatgc
      241 tgcgtacacc gatctctctc acggtgatgg aatgccaatt tcgccgtaca ggcactaatt
      301 aacagtagga tcgtcttcag agcaagatgg tcttcctgag actgactgac agataggcat
      361 tctccattct ctccccttgt ttttccacgc tctattccct tactctcgtc gcgcatcggc
      421 gtggagtttc ttgatgttgc aagctgtttc attacattgc agccgcggtg aacggcgatt
      481 gttttccgat ctcaacgtga aggttgagcg gggatcgcta ttggccgtgg taggtgaaaa
      541 cggcagtggg aaaaccagct tgctccgtat cttttccagt ttattgccgc ctgaacaagg
      601 ctcggttctg tgggaggggc aagatatcca tcaactcaag gagctctatt cgggtcaact
      661 gacatacatc gggcatctta atgggattaa ggatgacctc acgccgctgg aaaatctgat
      721 gagcgctatg gcgcttgccg gagaaccctg ttctcacagc ggagcgaagg aggcgttgga
      781 ggcgattggc ctcaaacgac ccatccatca tttgccgtcc aaagtgctct cgcaagggca
      841 gaaacgtcga gtggccctgg cgaagttatg gctctccacg cgtcccttgt ggctcttaga
      901 tgaaccgttc acttccctcg acactgcatc aacaagtatt gtcactcaac gtcttctcac
      961 gcacttacag cgtggggggc tggcagttgt tgtgacgcat caggaagtcg cgcttcctgc
     1021 cgagaccgtg caacgtctga ggctgacggg atgatcggtt ccagcatgtg ggaagccgtt
     1081 agtggtatcg ttcgaaggga cctgctcctt gctatgcggc gccgttcaga cgccgcgatg
     1141 tcggttttct ttcttgtgat cgtggtcagt ctgtttccct tgggtgttgg accggagccg
     1201 gcggtgctgc ggaccatcgg tcctggggtg ttgtgggtgg cggccttgtt ggcctgtctt
     1261 ttgtcgctag gacgtgtgtt tacggcggac tatttggacg gtgttctcga gcagatggtc
     1321 ctgattcccc aaccacttgc ggtattggtc gttggaaaag tcctcgcaca ttggctgatc
     1381 tccggactac ccgtcgttct gctctcacct ctcctgggac ttcaattcgg tttaagcggg
     1441 gaatcactgg gagtactggt gatgtcgctc tt
//
LOCUS       DASTWE010000877         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1477816, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000877 DASTWE010000000
VERSION     DASTWE010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477816"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFT30_09160"
     CDS             <1..786
                     /locus_tag="VFT30_09160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU5092842.1"
                     /translation="RSPSKITRRLGMTPLAARKLTTWIRFMYGYALGLVPDAPYVFRN
                     GARMRIGRGVDHVPLIEIFLREDYGSVADGAVVLDLGANIGTFAVYAGTTAKRVTVYA
                     YEPMSEFYRLLQDNIRLNHLEDAVRCFNRAVSSDSSERELVVSGPKVFFPTFFPQDRE
                     NSGSAVRVQCTTLAEIIDTNKLQRFDLLKMDCEGDEYDILYATPKAYFDRIAEIRLEY
                     HNLAGERRNVDSLTDFLRQVGYEVTVAKSANTDQMSGNLRAYR"
     gene            783..>1065
                     /locus_tag="VFT30_09165"
     CDS             783..>1065
                     /locus_tag="VFT30_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092843.1"
                     /translation="MTIDVSSYKENGFFLAKHFFAPEEVERVRGDAKAVFIAQMLRLN
                     IISTDQPSEHEFEHGMFQFFQTDIQSFTNCGKQAQHLISLHRLSLDDRII"
BASE COUNT          236 a          285 c          285 g          259 t
ORIGIN      
        1 cgctctccct caaagatcac gcggcgcctg ggcatgacgc ctctggctgc tcgcaagctg
       61 acgacctgga tccgtttcat gtacggctat gcgctcggct tggttccgga cgcgccctat
      121 gttttccgca atggcgctcg aatgcggata ggacgaggcg ttgatcacgt ccccctcatc
      181 gagattttct tgcgggagga ctatggctcg gtcgccgacg gggccgttgt cctggatctc
      241 ggtgccaata tcgggacttt cgccgtctat gccggcacaa ctgccaaaag agtcaccgtc
      301 tatgcgtacg agccaatgag cgagttctat cgtttgctgc aagacaacat tcgactcaat
      361 catctcgaag acgccgtccg atgtttcaat cgtgcggttt cgtccgattc gagcgagcgc
      421 gaattggtcg tgagtgggcc caaggttttc tttccaacat tttttcccca ggatcgggag
      481 aattccggca gcgcggttcg cgtgcaatgc acgactctcg ctgaaattat cgatacgaat
      541 aagctgcaac gtttcgacct gctgaagatg gattgtgaag gagacgagta cgatatcctt
      601 tacgccacgc ccaaggcgta ttttgatagg atcgctgaaa tccggctgga gtatcacaac
      661 ctggctggcg agcgccgtaa cgttgactct ttgaccgact ttctgcgtca ggtcggttat
      721 gaggtaaccg tcgcaaagag cgccaatacg gaccagatga gcggcaacct gcgagcctac
      781 agatgacgat cgatgtatca tcctacaaag agaatggctt ctttctggcg aagcatttct
      841 tcgcgccaga agaagtggag cgtgtccgcg gagatgcaaa agcggttttc atcgcgcaga
      901 tgctcagatt gaacattatt tcgacggacc agccatctga gcacgaattc gagcatggca
      961 tgttccaatt tttccagacc gatattcaaa gctttaccaa ttgtggcaag caggcccagc
     1021 acttgatctc tctgcacaga ttatcgctgg acgaccgtat cattt
//
LOCUS       DASTWE010000878         1444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_604751, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000878 DASTWE010000000
VERSION     DASTWE010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604751"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..1229
                     /locus_tag="VFT30_09170"
     CDS             432..1229
                     /locus_tag="VFT30_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092844.1"
                     /translation="MALVVATGSLNWTSDAFAVRPFVTDDARIVYKGQLEVESFAGVA
                     LANGSSPVVEARSIQGMSLTDRFEVIAGGFGFTYQDGQARPLDMLIQPKYVIHRSFGI
                     IPSISVAAASLFPLSGNKQHWDSYTMAHISWFLFTPENSTDPYDNDLAIHVNLGTKNR
                     YDAGPATYQMKFYWAAGFEVITFSRELRFLAETFNGDPFGFEEKFPAYQAGFRWYRTP
                     NMQLDFVFRGIRNTTDDVRLVAGGGGLPEEWNYTIQIGFRFLIDVFR"
     gene            1242..>1444
                     /locus_tag="VFT30_09175"
     CDS             1242..>1444
                     /locus_tag="VFT30_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092845.1"
                     /translation="MNPTVMIRWMLVVLIGSVGLSGCGLDGVTGTLIRLDGEWYSIRT
                     PKGEELRIHVDSHSRKDAVEAGDA"
BASE COUNT          320 a          340 c          427 g          357 t
ORIGIN      
        1 aggaactgat gcaaagcttc gcgcgctgat ggtgaaactt cttcccgatc ggatctccga
       61 ccgaatgctg acgtggatac tgaaactccc gcactgagcg gccagcgcgt tcgttgccta
      121 gcccttccaa tgtgagaagc ctcgtttctt ttcggacgca agtattcaat aaataggaac
      181 gcggttccga agcagattgt cgtgccggtt atggcatgcc gaccggccct tgactgaaga
      241 gtgacgcttc tcatgacggg aatgttaaat gtttgttgcg gttggaagcc tgatcgaatt
      301 ttaattgagg ttcaaggcaa tctcatgtaa tcaaagagct aagcaggtgt ccatgtgtga
      361 attcctgcca aaagacagtg agagaaccgg ttgtgagtat gcgtgtgcgt tgggttcagt
      421 gtctcggagt gatggcgctt gtcgtcgcaa cgggttcact caactggacg tcggatgcgt
      481 tcgctgtccg tccattcgtg accgacgacg cgcgaatcgt ctataagggg cagttggaag
      541 tagagtcctt cgccggcgtg gcattggcga acggcagcag cccggtcgta gaagctcgct
      601 caattcaggg catgtcgttg acggaccggt tcgaagtgat agccggggga tttgggttca
      661 cctatcaaga tggtcaagca cgtccgctcg acatgctcat ccagccgaag tacgtcattc
      721 accgatcgtt cgggatcatc ccatcaatat cggtcgctgc ggcatcgctc tttccgctca
      781 gcggaaacaa gcagcactgg gattcgtata cgatggcgca catcagttgg tttctcttta
      841 cgcccgagaa tagtacggac ccctacgaca acgacttggc catccatgtg aatcttggga
      901 ccaagaatcg gtatgacgcc ggcccggcga cttatcagat gaagttctat tgggcggctg
      961 gtttcgaggt gatcaccttc tcgcgcgaat tgcggttcct tgctgagaca ttcaacggtg
     1021 atccctttgg gtttgaggag aaatttccag cgtatcaagc gggattccgt tggtacagga
     1081 cgccgaacat gcagttagat tttgtctttc ggggaattcg gaacaccacg gacgatgtcc
     1141 gcctcgttgc gggagggggt ggcttgccgg aagaatggaa ctatactatt cagatagggt
     1201 ttcgatttct tatcgatgtc ttccggtagt caggcgtgag agtgaatccc acagtgatga
     1261 ttcgatggat gcttgtagta ttgataggca gtgtgggtct gtccggatgc ggattggacg
     1321 gtgtgaccgg aactctcatt cgattggatg gggagtggta cagcatccga acccccaaag
     1381 gagaagagtt acggatacat gtcgactccc attcgaggaa agatgccgtc gaagcaggcg
     1441 atgc
//
LOCUS       DASTWE010000879         1189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1316117, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000879 DASTWE010000000
VERSION     DASTWE010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316117"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1189)
                     /locus_tag="VFT30_09180"
     CDS             complement(<1..>1189)
                     /locus_tag="VFT30_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5092846.1"
                     /translation="YFHASPEQPGQPYCIVIPPPNVTGSLHVGHALNHSLQDILIRWR
                     RMQGRNTLWLPGTDHAGIATQNVVEKQLMAEGLSRESLGRERFVERVWQWKATSGNTI
                     VNQQKQLGESCDWDRLRFTMDEGLSKAVLEVFVRLYEDGLIYRGERLINWCPRCLTAL
                     SDIEVEHEDVKGKMYHIRYPLADDPNSSLIVATTRPETMLGDTAVAVHPDDPRYRHLI
                     GKKVRLPLTTREIPIVGDSILVDLEFGTGAVKITPAHDFNDYEAGERHKLPRLAVLDH
                     AALLDAAGMQRAAVESSVIEMLRGLSVSKARPKVETLLREQGVLDKTEDHKMSIGKCY
                     RCKTVVEPYLSPQWFVSIKPLAEPAIKAVEDRHIRIIPESWINNYLGWMRDIKDWCIS
                     RQIW"
BASE COUNT          252 a          332 c          317 g          288 t
ORIGIN      
        1 accaaatttg ccgtgagatg caccaatctt tgatatcccg catccagcca agatagttgt
       61 tgatccaact ttcaggaatg atgcggatgt gccggtcttc taccgctttg atcgccggtt
      121 ccgccagcgg cttgatgctc acaaaccatt gtggcgagag atatggttcc acgacggtct
      181 tgcagcggta acatttgcca atcgacatct tgtggtcttc ggttttatca agcacgcctt
      241 gttcacgaag caacgtttca accttcgggc gtgctttgga gacagaaagc ccacggagca
      301 tctcgattac cgatgattcg accgccgccc gttgcatccc cgcagcatcg agcaatgcgg
      361 catgatcgag cactgccagt cgaggaagtt tgtgccgctc tcccgcttca tagtcgttga
      421 agtcgtgcgc cggagtgatt ttcactgctc cggttccgaa ttcgagatcc acaagaattg
      481 aatcgccaac gattggaatc tcacgtgtcg tcagcggtaa gcgaactttc ttgccgatca
      541 gatggcgata ccgaggatcg tccgggtgca cggcgacggc cgtgtctccc agcatcgttt
      601 ctggccgcgt tgtggccacg attaaactgg agtttggatc atccgccagt ggatagcgga
      661 tgtgatacat cttgcctttt acatcttcgt gttccacctc gatatccgag agtgctgtga
      721 ggcagcgtgg acaccaattg atgagccgct caccacgata gatcaatccg tcttcataca
      781 gccgcacaaa gacctcgaga acggccttgg acaaaccttc gtccatcgta aatcgcaaac
      841 ggtcccagtc gcaggattct cccagctgct tttgttgatt gacgatcgtg ttaccggacg
      901 tcgctttcca ttgccagacc cgttcgacga atcgttcccg tcccagcgac tcccgtgaca
      961 ggccttcggc catcagctgc ttttccacca cgttctgcgt ggcgatgcct gcgtgatcgg
     1021 ttccgggcaa ccagagggta ttacgcccct gcatacgccg ccaccggatc aaaatgtctt
     1081 gaagcgaatg attgagtgca tggccgacat ggagcgagcc ggtcacattc ggaggaggaa
     1141 tgacgatgca atagggttgg ccgggttgtt ccggtgaggc gtggaaata
//
LOCUS       DASTWE010000880         1844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1445612, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000880 DASTWE010000000
VERSION     DASTWE010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445612"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1844
                     /locus_tag="VFT30_09185"
     CDS             <1..>1844
                     /locus_tag="VFT30_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092847.1"
                     /translation="IPALLQAIAVSLDWHVGLFWLVQNDRRTITCRWDWSVGTTAVQN
                     LVSVSRQQAYVSGFGLPGRCWASGEPVWVEDVARDPEFVQGPRDIGKMLHAACAFPVW
                     LRANVFGVMEFFSPDAQHPDWDLLKALGTVGSQIGLFIERTEVEAALHESEARTRLII
                     DTALEAVITTDRDGRISEWNAQAERIFGWSAHEVVGRNLADTIIPQSHRLRYREYVQG
                     LLEPRNLSLSNMLVEMIGLRRDNRQFPVEIAMSPLPVEGSIIFSAFIRDITNRKEAEQ
                     ALKDYAQRLEHINQQLDAALREAKAATEAKSTFLATMSHEIRTPMNGVIGMTGLLLDT
                     KLTDEQKEYAETVRTCGDHLLMIINDILDFSKIEAGKLDLEVIEFDLRLAIEESLDLM
                     AERASSKGLNLACLFHADVPRNVVGDPGRLRQIVMNLTANAIKFTDSGDIVVEVTAEA
                     QSKEEASIRVAVTDTGIGISEEGRERLFQPFSQADGSTTRKYGGTGLGLVICQRLVEM
                     MGGAIGVTSRVGEGSCFWFRVNLCKQAETSRGDESSAIALANLRILIVDDKAINRRIL
                     ELMTRKWGMRPTLVDSGPAALEIFSRHHGQPLFDVVLLDIDMGSTNGI"
BASE COUNT          419 a          462 c          560 g          403 t
ORIGIN      
        1 atcccggcgc ttctccaagc gattgcggta agcctcgact ggcacgtcgg cttgttttgg
       61 ttggtacaga acgatcgacg aaccattacc tgcaggtggg actggtctgt cggtaccacg
      121 gcggtgcaga atcttgtgag cgtcagcagg cagcaagctt atgtttccgg attcgggttg
      181 cccgggcgct gttgggcgag cggtgaaccg gtgtgggtcg aagatgtcgc acgagatccg
      241 gaattcgtcc aaggacctcg cgacatcgga aagatgttac acgcggcttg tgcatttccg
      301 gtctggttga gagccaacgt cttcggcgtg atggagttct tcagcccaga cgcgcagcat
      361 ccggactggg acttgctcaa ggcgttgggt acggtcggaa gccagatcgg tctgtttatc
      421 gaacggaccg aagtggaagc cgctttgcac gagagtgaag cccgtacacg gttgatcatc
      481 gatactgcgc tcgaggccgt gattaccacg gatcgcgacg ggcgaatcag cgaatggaat
      541 gcccaagccg agcggatatt cggctggtcc gcgcatgaag tcgtcggacg taatttggcc
      601 gataccatca ttccgcagag ccatcgcctc cgctaccgtg agtatgttca gggtctcctt
      661 gagcctagaa acctgtctct ctcgaacatg ctggtcgaga tgatcgggct tcgacgtgac
      721 aaccgacaat ttcccgttga aatcgccatg tcgccattgc ctgtcgaggg ctctatcatc
      781 ttcagcgcat tcattcgaga catcacgaac cggaaagagg cggagcaagc gctgaaggac
      841 tatgcccagc ggttggagca tatcaatcag caactggatg ccgcattgag agaagccaag
      901 gcagccactg aagccaagtc gactttcctg gcgaccatga gccacgaaat tcggaccccg
      961 atgaacggtg tcatcggtat gacgggtctc ttgctcgata cgaaattgac agatgaacag
     1021 aaggaatatg ctgagaccgt tcggacctgc ggtgaccatc tgctgatgat catcaacgac
     1081 attcttgatt tttcaaagat agaagcaggg aaacttgatc ttgaggtcat cgagtttgat
     1141 cttcgccttg ccatcgaaga atcgttggat ctcatggctg agcgagcctc atccaaggga
     1201 ctcaatctgg catgtctctt ccatgccgat gtgccgagga atgtggtcgg tgatccgggc
     1261 cgcctacgcc agatcgtgat gaacttgacg gccaacgcca tcaagtttac cgacagcggc
     1321 gatatcgtcg tggaggtgac tgccgaggct caatcaaagg aagaggcgag cattcgcgta
     1381 gcggtcacgg acaccggaat cgggatttcc gaagagggcc gtgaacggct ctttcaaccg
     1441 ttcagccaag ctgatgggtc gaccacgagg aaatatggag gaaccggcct cgggcttgtc
     1501 atttgccaac gtcttgttga gatgatggga ggggcgatcg gagtgacgag ccgagtcggg
     1561 gaagggagct gcttctggtt tagggtgaat ctttgcaagc aggccgagac ctcgcgtggc
     1621 gacgaatctt ccgccattgc gttggcgaat ctccgcatct tgatcgtgga tgacaaggcc
     1681 attaatcgaa ggatcttgga attgatgaca agaaaatggg gaatgcggcc gacacttgtc
     1741 gacagcggcc cagcggcgtt ggaaatcttc agccgccatc acgggcagcc actgtttgat
     1801 gtcgtgctat tggacataga tatgggatcg acgaatggaa tcga
//
LOCUS       DASTWE010000881         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_185190, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000881 DASTWE010000000
VERSION     DASTWE010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185190"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /gene="ispF"
                     /locus_tag="VFT30_09190"
     CDS             <1..466
                     /gene="ispF"
                     /locus_tag="VFT30_09190"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU5092848.1"
                     /translation="GYGYDVHPLGPGRKLILGGIEIPHTKGLLGHSDSDVLVHAVCDA
                     LLGAMGEGDLGRHYPSSDPQYKGISSLKLLEDVMAKLKAKGYRVGNIDTVIVAQAPRL
                     GPHLPAMQKKIAETAGIDPDLVNVKVKSGEGLDAVGREEGVIAHAVCLIEPV"
     gene            486..1124
                     /gene="cysE"
                     /locus_tag="VFT30_09195"
     CDS             486..1124
                     /gene="cysE"
                     /locus_tag="VFT30_09195"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU5092849.1"
                     /translation="MLAKIKQDLQAVFDRDPAATSKLEVILTYAGFHALLTYRIAHWL
                     KAYDVPILPRVISQLARWVTGVEIHPSAKIGTGFFIDHGMGVVIGETAEIGDYVTLFQ
                     GVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGIKIGDNVKIGANSVVLKNVPPNSTV
                     IGVPARVIKTQGERLPDATMDQVDLPDPISDRFIALEQELIELRKKVDDRNS"
     gene            complement(1388..1708)
                     /locus_tag="VFT30_09200"
     CDS             complement(1388..1708)
                     /locus_tag="VFT30_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEU5092850.1"
                     /translation="MVISLRALILGLFVLSLGVTPFAVAAPAQQNKMKACNEQANAKE
                     FGEGKGEERKAFMKECLSAKSEKSGGGKDTQQNKMKTCNKEAGEKNLKGEERKKFMSD
                     CLSA"
     gene            complement(1804..>2111)
                     /locus_tag="VFT30_09205"
     CDS             complement(1804..>2111)
                     /locus_tag="VFT30_09205"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEU5092851.1"
                     /translation="LVLIGPHDFSSFQTQPTDNDDPICHLQRFTVFREGDRLQIEAYA
                     NRFLKQMIRSIVGTLVEIGSSKRTPESLRTILNARDRSAAGKTAPPQGLFLMRVDYD"
BASE COUNT          474 a          550 c          593 g          494 t
ORIGIN      
        1 cggttatggc tacgatgtcc atccgctcgg tcctggccgt aagctgatct tgggcggtat
       61 tgagatcccc cacacaaaag gactgctcgg ccattctgat tccgatgtcc ttgttcacgc
      121 ggtttgcgat gccctattgg gggcgatggg ggaaggggat ctcggccggc actatccaag
      181 ttccgacccg cagtataagg ggatctccag cttgaagctg ttggaagatg tcatggcgaa
      241 gttgaaagca aagggatatc gagtgggaaa catcgacact gtgattgtgg cacaggcccc
      301 tcggcttggc ccgcatcttc cagcgatgca gaagaaaata gcggagaccg ccggcatcga
      361 tccggatctg gtcaatgtaa aggtaaaaag tggggaagga ttggatgcgg tcggacgcga
      421 agagggggtg atcgctcacg ccgtgtgttt gatcgaacca gtttgacgtc gtaagatacc
      481 acatgatatt ggcgaaaatt aaacaagacc tccaagcggt ctttgaccgg gatccggcag
      541 ccacgagcaa gctggaagtg atcctcacct atgctgggtt ccatgcgctt ctgacgtatc
      601 gcatcgctca ctggttgaaa gcgtacgatg tcccgatcct gccgcgggtc atttcgcagt
      661 tggcccgctg ggtgaccggc gtggagatcc atccatccgc aaaaatcggg accggctttt
      721 ttatcgacca cgggatggga gttgtgatcg gcgagacagc tgagatcggc gactatgtga
      781 ctctttttca aggcgtgact ttgggtggaa cgggaaaaga aagaggcaaa cgacatccta
      841 cgctcggaaa tcacgtcgtt gtcggggctg gggcaaagat tctggggggc atcaagatcg
      901 gcgataacgt caagatcggg gccaattccg tcgttttaaa gaatgtcccc cccaattcta
      961 ctgttatcgg tgtgccggct cgcgtgatca agacacaggg tgaacgtctg ccggacgcga
     1021 cgatggatca ggtcgatctg ccggacccca tcagcgatcg ttttatcgcg ctcgaacagg
     1081 aattgatcga actccgcaaa aaagtcgacg accgcaactc ctgactgatg ggtaaccctg
     1141 attctctggc ctccactcgc tggctcgata ctgcaaagct tttgtgatca gacaggaacg
     1201 acagtcaaga atccaggttg actctctctt tccttgaaaa gcaggtgcgc caccgttctg
     1261 ttgatgcgga agggaattct accgaaatct gtactgctgt tgccggcgtc catatcaacg
     1321 atttgggatt gtcgataccg acttcgttga ttccagcgcg gcatcatgcc gcgctggaat
     1381 aacagaatta tgcagagaga cagtcgctca tgaatttctt tcgctcctca cccttcaagt
     1441 tcttttcgcc cgcctcttta ttgcacgtct tcatcttatt ctgctgagtg tccttgccgc
     1501 ctccgctttt ctccgacttg gcggagagac attccttcat aaaggccttc cgttcctcgc
     1561 ctttcccctc gccaaactcc ttcgcattcg cctgttcatt gcaggccttc atcttatttt
     1621 gctgcgcagg cgcggccacg gcgaatggag tcactccaag actgagcaca aacaacccga
     1681 gtatgagagc acgcaaggat atcaccatgc tggatctcct tctgtagtga gggatgaggt
     1741 gcaagtctgg accgcatcat agcaaaacaa tacgtaattg tctgtaaatg aacaccgcaa
     1801 gggtcaatcg tagtcgaccc gcatcaaaaa gagtccctgt gggggagctg ttttaccggc
     1861 agccgagcga tcacgcgcgt tgaggatggt cctgaggctc tccggtgtgc gtttgctcga
     1921 gccgatctcc acgagcgtcc cgacgatcga gcggatcatt tgcttcaaga accggttggc
     1981 ataggcttcg atctgcagcc gatcgccttc gcgaaagacc gtgaatcgct gcagatgaca
     2041 gatgggatcg tcgttgtcgg tgggttgggt ctggaacgaa gaaaaatcgt gcgggccgat
     2101 cagaacgagt c
//
LOCUS       DASTWE010000882         1082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_573249, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000882 DASTWE010000000
VERSION     DASTWE010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573249"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFT30_09210"
     CDS             <1..295
                     /locus_tag="VFT30_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092852.1"
                     /translation="WFVYSSTTDSPQGMGNRDVISDFDGNGGADGDEINLVAIDGNLN
                     MIGHQALTAGQVSYNTGNGNLNVNVIGDPGAIDLQISLAGAPPVNLADDIILA"
BASE COUNT          262 a          293 c          264 g          263 t
ORIGIN      
        1 ctggttcgtc tacagctcga cgacagacag cccccagggc atgggaaatc gcgacgtaat
       61 aagcgacttt gacggcaatg ggggtgccga tggagatgaa attaatcttg ttgcaatcga
      121 cggaaatttg aatatgatcg gacatcaagc ccttaccgcg ggtcaagtgt cgtataacac
      181 cggcaacgga aatttgaatg tcaacgtgat cggtgatcct ggtgccatcg atttgcagat
      241 cagtttggct ggggcgcctc ccgtcaacct ggcagacgat atcatccttg catgatgatg
      301 taagcacgct tccaaatata tgtcagcgtg tactcaaggt ctcacgtgca cgctggctaa
      361 tctcatggca tttttcgccg gtcgagaccg agtctggcga tcaggcaata agacactttg
      421 ctttcgtcgc gataattcct gtaacgggaa ttgatttgtt acacggagga ctcctcatga
      481 ctcgattcaa taaaacctcg aaccgacaac gctcggttcc atagcgctga gcaggacgcc
      541 cctcctgtag ttgctcggca tgattaccaa accggaggcc catgctagca catcgcccct
      601 cctccctgcc attgcacggt gacgtgccgc gagatttttt atgcgttcca tggccggatt
      661 cagcagcgct tggacccgga ttcaggtgat ttgccggcat tcatccctat ccccagccca
      721 gcgccctctg tacagaacct attccatcac ttttccgtaa gttcccgggt cgcgcaagaa
      781 tttccagcgc gcttcactag gcgcgcttag taataccgct tcaggatgcc gggtctcttt
      841 atccgccggc gtttcggtta aggaatacta ggaagatcat gttgagagcg aactacgaaa
      901 aatcacgcga agcatctctc atactcatcc gatccgtcgc tattcaacga catcgttaag
      961 tattagtgat gatggctgaa aactctcggc cagcgaaata cacctgcaat acgtcgtcaa
     1021 tgcgcgacac acaagctcat aagattataa aggttaagag gcgctgcctg agcctcatct
     1081 tg
//
LOCUS       DASTWE010000883         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_378864, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000883 DASTWE010000000
VERSION     DASTWE010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378864"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..2470
                     /locus_tag="VFT30_09215"
     CDS             53..2470
                     /locus_tag="VFT30_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5092853.1"
                     /translation="MYPFVRRALGTWLMMIALTAVCGTLSIVQAEDNERALAIKGVVQ
                     NQDLRRVPQAVIEVKSQEGEIVSSGVSNDAGEFKISVPDRGTYSVSAVQETYRSEYVV
                     LTLSEEPPKPVTLTLSKTKEVALEVVSPLPPIQAKASSETYSLNRREIDVLPRGNNIN
                     LEDLLLTIPSATYGSLKQVHIRQDHANLQFRIDGVPIPDTVSSTFSDVITPRAWERAD
                     IVLGGMEANVGNKTTALLDITTKSGTKPGFGSVQMFGGSNNTINPSLEYGGTIGQKFR
                     YYFLNSHTATNRGIEPPTLGHSIFHGQSERNQTMFRGDYQHDNNNNFTLLFLNSIAKY
                     QIPTVPGQALNPTIVGLLPGGFTPVPSEMVDENQKENNQYGHVVWRHDINTRNFFSLA
                     GYFRQTRATFRTDPFNVLAYVPDETEPFSAGSQDRTGYSGGVRFDYTFVHSKEHVIKA
                     GFQIDRTQAINKTRLFTFIDDGVGNPTGGVINVNADNRLIGWRQEFWIQDQWTPNEHW
                     TFNVGVRGDAVQYQRDEGQISPRVGVTYRYNPAHAFHAFYGRLFTPPNLEAISFAKLN
                     TEGTKAAPEDTTDNLPRAERAHYFQIGSTHALTDWATLQLTGYYKLANYLSDAGQFGT
                     TPLLNYFAFERGWSRGADAALKVWFMENLTGRGNIAWGQCKGYGLQSGHFLLEATEIA
                     DIRTSSGIHCDHQQTLTASGILSYRLFERTTITGQMLLASGLRTAEEGGKTNSTHSPS
                     YTIYNFSIAHVIPLPWHDQKLLLAFDIVNALEQQYFINHGEGSIGLGVSHAGMPRSFF
                     FRGQLFF"
BASE COUNT          595 a          641 c          670 g          566 t
ORIGIN      
        1 tattttatca ttctcattta ataatcgctg gcttgtgagt gaggaggggt ccatgtatcc
       61 atttgtgaga cgtgcattag gcacgtggtt gatgatgatc gcactaactg ctgtatgcgg
      121 gacgttgtca atcgttcagg cagaggacaa cgaacgagcc ttggccatca agggagtcgt
      181 ccaaaatcag gaccttcggc gcgtgccgca ggcggtcatc gaagtgaaga gtcaagaagg
      241 cgaaatcgta tcatccggtg tctcgaacga tgccggtgaa ttcaagattt ccgttccgga
      301 tagaggtacg tactccgtca gcgccgtgca agagacctat cggagcgaat atgtcgtgct
      361 gacattgagc gaggagccac cgaagcccgt gaccctcacc ctttcaaaga cgaaagaagt
      421 cgccctggag gttgtgtcac cgttgccgcc cattcaggcg aaggcgtcaa gcgaaaccta
      481 ttcactcaat cggagagaaa tcgacgtcct tccgcgcggg aacaacatca acctagaaga
      541 cctgctgttg acgatcccaa gcgcaaccta cgggtctttg aaacaggtcc atatccgcca
      601 ggaccacgcc aaccttcaat ttcgtatcga tggcgtcccg attccagaca cagtatcttc
      661 cacattttcc gatgtgatta cgccccgagc ctgggagcgg gccgatattg tgctgggcgg
      721 tatggaggca aatgtcggga ataaaaccac agcactcctg gatattacga ccaaaagcgg
      781 tacgaagccg gggttcgggt cggttcaaat gttcggcggg tcgaataata cgatcaatcc
      841 gtccttggaa tatggaggga cgatcggcca gaaattccgc tactatttcc tgaacagtca
      901 tacggcgacg aatcggggca ttgagccgcc aactttggga cattccatct ttcacggcca
      961 gagcgagcgc aaccagacga tgttccgcgg tgattatcag cacgataaca acaacaattt
     1021 tacactgctg ttcctgaact cgatcgcgaa atatcagatc cccaccgtcc caggacaggc
     1081 gctgaatccg accattgtcg gcctgttgcc gggcgggttt actccggtgc cgtcggaaat
     1141 ggtggatgag aatcaaaagg agaacaacca atatggccat gtggtgtgga gacacgacat
     1201 taatacccgc aattttttca gtctggccgg atattttcgc caaacaaggg ctacgttcag
     1261 gacggatccg tttaatgtcc tggcctatgt gccggatgaa accgaaccct tttccgccgg
     1321 cagtcaagat cgcactggat attccggtgg agtgcgtttc gattacacct ttgtccatag
     1381 taaggaacat gtgatcaagg ccggatttca gatcgatcgc acccaggcga tcaacaagac
     1441 gagattgttt acgtttatcg atgacggggt gggcaatccg acaggaggag tgatcaatgt
     1501 caatgccgac aatcgattga tcggctggcg acaggagttt tggattcagg atcaatggac
     1561 tcccaatgag cactggacct tcaacgtggg cgtccgcggt gacgctgtgc agtaccaacg
     1621 cgacgaaggc caaatcagtc cacgagtcgg ggttacatat cgatacaatc cggctcatgc
     1681 cttccacgcg ttctatggcc ggttgtttac tccccccaat ttggaagcga tttcctttgc
     1741 caagttgaat acggaaggga cgaaagcggc tccggaagat actaccgaca atcttcctcg
     1801 ggctgagcgt gcccattact ttcagatcgg cagcacccat gccttgacgg attgggcgac
     1861 ccttcaactg accggctatt acaagctggc caactatctt tccgatgcag gccagttcgg
     1921 gacgaccccg ttgttgaact attttgcctt tgaacgaggt tggagccggg gggctgatgc
     1981 cgcgctcaag gtgtggttca tggagaatct gaccggacgt ggaaacattg cttggggaca
     2041 gtgtaaaggg tatggactcc aatccggcca tttcttattg gaggcaaccg agatcgcgga
     2101 tatcaggaca tccagcggca ttcattgcga tcaccagcag acgctgactg catcgggaat
     2161 cctgagctac cgattgttcg agcgaacgac catcaccggg cagatgctgc ttgcctcagg
     2221 gctgcggacg gcggaggaag gtggaaagac caactcgacc catagtccgt cctacacgat
     2281 ctataacttt tctatcgcgc atgtcattcc gttgccctgg catgatcaga aattgctgct
     2341 ggcgttcgat atcgtcaatg cgcttgaaca acagtacttc attaaccatg gcgaaggcag
     2401 tatcggcctc ggtgtgtctc atgccggcat gccgcgatcc ttcttcttcc gtgggcaact
     2461 gttcttctga ta
//
LOCUS       DASTWE010000884         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_637602, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000884 DASTWE010000000
VERSION     DASTWE010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637602"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..560
                     /locus_tag="VFT30_09220"
     CDS             30..560
                     /locus_tag="VFT30_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092854.1"
                     /translation="MVRVCVGVKPVSADALDVVSEEVGQELVSRCGEHAKGAVQWLFH
                     AQLREHARRDPVRRGPYRHLARAVHRIRIAPPRFGASDTGSDTAARSEAQQRPRQLAL
                     RSRTTWRLFPVERQSGRPKVFQYTAINDYTRNEVFRLYTLKTRGPAIRFYTTFPIPKL
                     QADNDTEFPLLFAFTG"
     gene            complement(892..1542)
                     /locus_tag="VFT30_09225"
     CDS             complement(892..1542)
                     /locus_tag="VFT30_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU5092855.1"
                     /translation="MLMHETLIQLVGRKSIGSIFHLMLLSVILLPLASCTGGDGDTGP
                     MISSLATPTEDATTDSNSEPSLDDTDLNLGGEEPMPFSEEEADTNSAAAEVNSDSSVS
                     DSEGDEDLIASLPTPPDDNPTISMTSTPTGATANLTWQSASDPKRKGYYVYYGKQPSE
                     SPGVCSYEERIAVDTPPVTIAELEPNTPYFFAVSAYNNFESPCSNEATAITPPAGT"
     gene            complement(1810..>2283)
                     /locus_tag="VFT30_09230"
     CDS             complement(1810..>2283)
                     /locus_tag="VFT30_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092856.1"
                     /translation="IAIPFGIGIGTLYSVTTISGPPLALLFNNQGYAKQDFRAALGVI
                     RVAESSLTAIAYGFIGFYSAGSMDIIPYIVPSVIVGIPLGTYLIRLIDPETFRRICMA
                     FDGLVVGFGLSRVLVELDLATPTTTYSILSIVVLINGYLLFRFFRDRVNPQQIPS"
BASE COUNT          572 a          567 c          601 g          543 t
ORIGIN      
        1 cctgatcgat gccaagggat gaggtgccga tggtgcgagt gtgcgtgggc gtcaagccgg
       61 tctcggcgga cgcactggac gtggtcagtg aagaagtcgg tcaagaattg gtgagccgtt
      121 gtggtgagca tgcgaaaggt gccgtccaat ggctctttca cgctcagctg cgtgagcacg
      181 cccgccgcga tccggtacgg agggggccat atcggcattt ggctcgagcc gtccatcgga
      241 tccggatcgc cccgccgcga ttcggcgcat ccgataccgg ctcggatacc gcagctcggt
      301 cagaagccca gcagcgcccg cgtcaactcg ctcttcggtc gcgaacgacc tggagattgt
      361 ttccagtgga acgtcaaagt ggccggccca aagttttcca atacaccgcc atcaatgact
      421 acactcgcaa tgaggtcttc cgactgtata cactgaaaac ccgcggacca gccatacgtt
      481 tctacacgac gtttcccatc ccgaaactac aggccgataa cgacaccgaa ttcccactct
      541 tatttgcgtt cacaggctaa caggcggatg tggccgcgct atattaaacc tcgatgtccg
      601 tgtagaacgg gaaagtcgaa tggagtcacc gtatcgatga agaggaatta ttggagttcc
      661 cgatatttgc cgagttctca caacgttcgc cgcggcttct tcacccgctc cgccatcata
      721 gtcctccaga gaatcactta gacccgccgc aactccttaa cactcatcgc cacacggcca
      781 tactatcctc cgactatccc actctctttt gagagcggcc agcccatcaa tgaagaggac
      841 atttctactt cggcgaaact ggaattatca ttgtgggctc acactacatg ttcaggttcc
      901 agcgggaggc gttatcgctg tggcttcatt cgagcaaggg ctttcaaagt tattgtaagc
      961 actgaccgcg aagaaatatg gtgtgttggg ttcaagttcg gcgatcgtga caggtggagt
     1021 atcaacagct atccgctcct cataagagca aacacccgga ctttcggatg gctgttttcc
     1081 gtagtagaca tagtagcctt tcctcttggg atcagaggct gattgccatg ttaaattggc
     1141 ggttgctcct gtgggtgttg aagtcattga aatggtcggg ttgtcgtcgg gtggggtggg
     1201 taaagaagct atgaggtctt catctccctc ggaatctgat acgctagagt cactattcac
     1261 ctcagcagcc gccgaattgg tatctgcctc ctcttccgag aagggcattg gttcttctcc
     1321 acccagattt agatcagtat catcaaggga aggctccgag tttgaatctg tcgtggcgtc
     1381 ctccgttggt gttgcgagac tagaaatcat gggtcctgta tctccatcac caccagtaca
     1441 gctggccagc ggtaagagaa tgacactcaa cagcatgaga tggaaaattg aaccaataga
     1501 ctttcggccg acaagttgaa tcaacgtttc atgcattaac attgaaaact ccctctatgg
     1561 tggatgattt atggccttaa gctcggggtc ttcctagggt tggatgggac tagcaaaaga
     1621 aatgccatct ggatttaata aaaaaatatt ttagtttcaa tgcgttgcaa tcttcgttgc
     1681 accacgaaaa tgaaggagtt gaagggttca accttcaagt actccaacag ccatggcgga
     1741 gtagtgcttg agccgagcgg caggtttgtc cagctcaaaa ttgttgtgat gaaagactaa
     1801 cgtgagggat caagatggga tctgttgggg atttactcga tctctaaaaa atcgaaacaa
     1861 taggtagcca tttatgagaa ccacgatcga caagatactg taggttgttg tgggtgtggc
     1921 aagatcgagt tctactagca cccgagacag gccaaatccg accactaacc catcgaatgc
     1981 catgcaaatg cgcctgaacg tttcgggatc tatcaatcgg atgagatagg ttccgagtgg
     2041 aatacccacg ataacgctcg gaacgatgta ggggataata tccatactgc cggcactgta
     2101 gaagccgatg aatccatacg caatcgctgt gagagaggat tcagcgacac ggatgactcc
     2161 caaggcagcc cggaaatctt gtttggcgta tccctgatta ttgaagagca gcgcaagagg
     2221 gggaccggaa atggttgtga ctgaatagag tgtcccaatt ccaattccaa acggaatcgc
     2281 aat
//
LOCUS       DASTWE010000885         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1445716, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000885 DASTWE010000000
VERSION     DASTWE010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445716"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..>1733)
                     /locus_tag="VFT30_09235"
     CDS             complement(180..>1733)
                     /locus_tag="VFT30_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEU5092857.1"
                     /translation="QKQEQYRDLMIRTFYLSTAKAKDMLLLLKSMLDSKRMHANEQLN
                     AIVIRDQPEKLELAEKIILANDRQEAEVLFDLEVLEVNRTKSQTYGLNYPKQAGAGLV
                     AAGFAGPLAAEAVHMTYRQLTDLGPSNYLFKLPTTVLLDFFKQQSDAKTLASPKVRVM
                     NNKKAEINIGDKQPILLSTTNVLPGQAATGAVPTTSTVTSIEFRDTGVKLTVEPSIRL
                     GNELSLKMKVEVVRVGDPVLLQASPKIEQFKFGNRSAETTLNMRDGETIVLGGLLQEE
                     DRRTRVTVPWIGDLPLIGKLLSSFKTDRVTTEVILTITPRIAQPALPPGSSNHAFWSG
                     TEFNYATSPIFSNPSRKVSALMGSEDGKVSTQGGAITKDAGQTNIVPSLAQFAALDPL
                     LAIKPEESVVQSGKEIRLSVVDGQISGSREHTFKLGYDPKILQFNRLDNAELIDMGET
                     TTADNGGQEGTVTFHFARSVQRLPRTMNVIFVAKAPGVSPIRVELTDSGGAAQTSPEV
                     VGTGVVRVR"
BASE COUNT          398 a          473 c          416 g          446 t
ORIGIN      
        1 gcgattccat tccatcttaa acagatcgat tgcgccacga atcgttcgaa gatgctggcg
       61 cagctcaatc tcctgggctc gctatgtcga tactttactc agaggcatgg tgaccgaagc
      121 caagatcaca ataatcgtca tggtgactag aatctcgatg agtgttaggc cgtcctccct
      181 caccgcactc gcaccacgcc tgtcccgact acttctggcg atgtttgcgc cgcaccacca
      241 gaatcagtca gttctacacg aatcggcgat acccctggcg ctttggccac gaaaatgaca
      301 ttcatggttc gtggcaaacg ctgaacggaa cgagcgaagt gaaacgtgac ggttccttct
      361 tgccctccat tgtccgccgt tgtggtctcc cccatatcaa tcaactccgc attatcaagc
      421 cgattaaatt gtagaatctt ggggtcatag cccagtttga atgtgtgttc gcgagatccg
      481 ctgatttgcc catctacaac agaaagtcgt atttctttac cggattgaac aaccgattct
      541 tccggcttga tcgccaataa aggatctagt gctgcaaact gggccaatga tgggacgatg
      601 tttgtctgcc ctgcgtcttt cgtgatagca ccaccctggg tagacacctt cccgtcctcg
      661 cttcccatca aagccgacac ctttctcgag gggttggaaa agatcgggct ggtcgcatag
      721 ttgaattcgg taccggacca gaaggcgtga ttgctggatc cgggaggaag cgccggttgt
      781 gcaatgcggg gagtaatagt caaaatgact tctgtcgtaa cacgatcggt tttgaaagag
      841 ctcagcagtt ttccgatcag aggcaaatcc ccaatccatg gcacggtcac ccgtgttcga
      901 cgatcttctt cctgaagtaa cccgccgagg acgatcgtct ctccatcccg catattcagc
      961 gtcgtttctg ccgaccgatt accgaacttg aactgttcaa tcttcggaga agcttggagg
     1021 aggaccggat ctccaacccg cacgacctcg accttcatct ttaacgacaa ttcattacct
     1081 aatcggattg aaggctcgac ggtcagttta actcccgtat ctcggaattc gatcgacgtg
     1141 acggtcgagg tggtcggtac ggctcctgta gcagcctgcc caggcaagac attggtggtg
     1201 gagagaagaa tcggttgttt atctccgata ttgatctcag ctttcttgtt attcatcacg
     1261 cggactttag gtgaagcgag agtcttggca tcggattgct gcttaaagaa gtccagcaac
     1321 acagtcgtcg gcagcttaaa gagatagttg ctcggcccaa gatccgtcag ttgtcggtaa
     1381 gtcatgtgta cggcctctgc cgccaatggt cctgcgaacc cagcagcgac caaccccgca
     1441 ccagcttgtt tcggataatt caacccatat gtctgactct tcgtccgatt gacttccaac
     1501 acttcgagat caaacagcac ctctgcttcc tgtcggtcat tggcaagaat gatcttttct
     1561 gcaagctcca acttctccgg ctgatctcga ataacgatgg cattgagctg ctcgtttgcg
     1621 tgcattcgct tcgaatccag catactcttc agcaggagca gcatatcttt ggctttcgca
     1681 gtcgaaagat aaaatgtccg aatcatcaga tctcggtact gctcctgttt ctg
//
LOCUS       DASTWE010000886         1095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_896537, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000886 DASTWE010000000
VERSION     DASTWE010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896537"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..>1095)
                     /locus_tag="VFT30_09240"
     CDS             complement(222..>1095)
                     /locus_tag="VFT30_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEU5092858.1"
                     /translation="LLLVFFPGHSQSEEPPKASKTLRDNAAIEREIGQAGERKGDVYK
                     ISFPRTDLNVTVDGVTVKPGLALGSWMAFRPTGKTTIVDGDLVLTEDEVYPVLRELQQ
                     HGLTVTALHNHLLQESPKIMYLHFWGEGDAAKLSSGLKQALSRTKTPVPPPQEENTKT
                     EDGFDAEQVQQILGQKGALKRGVLHVSVPRPEKITVHGVELPPSMGMATVMNFQAAGD
                     GKVAATGDFVMIDEDVNSVAQALTEQGIRITALHNHLLHDSPDLFFMHFWAHDFSEKV
                     AQGLRAWLDAMNKK"
BASE COUNT          239 a          290 c          281 g          285 t
ORIGIN      
        1 cacagcgcca atctacaaac acccgtaata cgagccattt gatacgtcat tgagtcatgc
       61 gtgctaggga atttgtagaa tgtcagctaa ctcaattttc ttcggaatag gcgaagagga
      121 gcaaaccaaa gaggatgatc gatcccgccc aggcaatttc catccacggt gtcactaggg
      181 ctggactagg ccttcaccgt tctagttgca gttcctcaac gttatttttt attcatcgca
      241 tcgagccagg ctctcagccc ttgtgcgacc ttctcggaga aatcatgtgc ccagaaatgc
      301 atgaaaaaca gatcgggaga gtcatgaagc agatgattat gaagggccgt tatcctgatc
      361 ccctgttctg tcaatgcttg agccactgaa ttgacgtctt cgtcgatcat gacgaaatcg
      421 ccggtcgccg ccactttccc atcgccggcc gcctgaaagt tcatgactgt agccatgccc
      481 atgctgggcg gaagttctac cccatgcacg gttatcttct ctggtctcgg caccgaaaca
      541 tggagcacgc ctcgtttgag ggctcccttc tgaccaagga tttgctgaac ctgttccgca
      601 tcgaagccgt cttccgtctt ggtattttct tcctgaggag ggggcactgg tgtcttcgtt
      661 cggcttaagg cttgcttgag accgctactt agtttggcgg catcgccttc tccccagaaa
      721 tggagataca tgatctttgg cgactcctgt aatagatgat tatggagagc ggtcactgta
      781 aggccatgtt gctgaagctc acggagtacg gggtagactt catcttcggt gaggaccaaa
      841 tctccatcga caatagttgt cttgcccgtg ggtctaaatg ccatccagct tccgagcgcg
      901 agtcctggtt tcaccgtgac gccgtcgacg gtcacgttga gatcggtccg cgggaaagaa
      961 attttgtaca cgtcgccttt gcgttctccg gcttgcccta tttcgcgctc aatcgccgcg
     1021 ttatcccgca aagtcttcga tgcctttggg ggttcttctg attgagagtg gccagggaaa
     1081 aagacgagca gcaag
//
LOCUS       DASTWE010000887         1734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_573321, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000887 DASTWE010000000
VERSION     DASTWE010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573321"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFT30_09245"
     CDS             complement(<1..660)
                     /locus_tag="VFT30_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5092859.1"
                     /translation="METVRLEPIRNVAIVSSAGAGKTSLSEALLYVGGAIPMLGSVTQ
                     GTTVSDFEPEELRHRNSTSTSLLQFSWNHTNINLIDPPGALDLLGEPLAALQAVDAAI
                     LLLSGNTGVRTELARLWGRIKELDLPCLVFVNGLDKEGASFENALETCRQQFGRIPIP
                     MTLPITIGAHLDGIIDVRHEKVVRSGPSSAQVEHVQISNDLEGILSGARKQLVEAVAE
                     SG"
     gene            complement(738..1463)
                     /gene="radC"
                     /locus_tag="VFT30_09250"
     CDS             complement(738..1463)
                     /gene="radC"
                     /locus_tag="VFT30_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU5092860.1"
                     /translation="MAGRGQRKGIAYWPRAERPRERLLAKGSEALSDAHLLAILLRTG
                     RRDSSAVQVAIELLDRVGGLGGLAACGIEELCAIPGIGPAKAAQLKAAVALGRRSLAV
                     PMSTGTRISSSADLFKHFHPALRDVKHELFKVVLLDAKNIVIKEATVSEGSLTLSIVH
                     PREVFALAVRESAAAVIFLHNHPSGDPTPSPEDRRLTDRLATAGQLLGIRVLDHVIIG
                     DGRYVSFADEGWMTEQRNECEAS"
BASE COUNT          394 a          473 c          465 g          402 t
ORIGIN      
        1 tccgctctca gcgactgctt ccacgagctg cttacgcgct ccgctgagga ttccctccag
       61 gtcgtttgaa atctggacgt gttcaacttg agcggaactc ggcccagacc gtacaacttt
      121 ttcatgccgc acatcaatta tgccgtctaa gtgcgcgccg attgtgatcg gtagtgtcat
      181 agggattgga atacgaccga actgttggcg gcaggtctcc agagcgttct cgaacgatgc
      241 accttcctta tcaagtccgt tcacgaacac caggcaagga agatccaact ctttaattct
      301 cccccacagc cgtgccagct ctgtcctcac cccggtattt ccgctcagaa gaaggatggc
      361 cgcatcgacg gcctgcaggg ctgcaagcgg ttcgccaagg agatcgagtg cgccgggggg
      421 gtcaatcaga ttgatgttgg tgtgattcca cgagaactga aggagactgg tacttgtcga
      481 attgcgatga cgaagctctt ccggctcgaa atcagaaacg gtggtaccct gtgtcacgga
      541 accaagcatg gggattgctc caccgacata cagcaaggcc tcactgagag aggtcttgcc
      601 cgcaccggcg ctggatacga tcgccacgtt tctgatgggc tccagacgga ctgtttccat
      661 agtctgccct ccttgttgag tatggagtta ggccattcgc ttcctcattt cctaggcatt
      721 ctttcgttcg acatttttca ggaggcctcg cactcatttc tctgctctgt catccatcct
      781 tcgtcggcaa aactcacgta acgaccatct ccaataatca catgatccaa gacgcggatc
      841 cccaacaact ggccggccgt tgctagtcga tcggtcaatc gacgatcctc cgggcttgga
      901 gttgggtctc cgctgggatg attgtgaaga aaaataacgg cggcggcaga ttcacgcacg
      961 gcgagcgcaa agacctcacg aggatgcacg atgctgagcg tgaggcttcc ctctgaaacc
     1021 gtggcctctt tgatcacgat gttttttgcg tccagcagca cgaccttgaa gagctcatgc
     1081 ttcacatcgc ggagggcagg gtgaaaatgt ttgaacagat cagcacttga ggaaatacgc
     1141 gtgccggtgg acatgggaac ggctagcgac cgtcgcccta acgccaccgc agccttgagt
     1201 tgggctgctt tggcaggacc gatgccaggg atggcgcaga gttcttcaat gccgcaagcc
     1261 gccaatcctc ccagcccacc gactcggtcg aggagttcga tggccacttg cacggcggac
     1321 gaatcgcggc ggccggttct caggaggatg gcaagtagat gggcatcgga taaggcttca
     1381 gatcctttgg ccagaagacg ctcacgagga cgttcggccc tcggccagta cgcaatgcct
     1441 tttctctgtc ctctgcctgc catggagacc ctcagatgac aaaaaattga cggaccggac
     1501 tcttttgtac tggcaaataa ggcgaaatta tcaccctaag gcaaatacga aattcataac
     1561 tcattgaaat actggaatat gacaaattgg tgcagctttt gcttaacttt gccaccagct
     1621 gtgaacgtag ccaccctggg agggatcaat ggaatgtccg aagtgcaaag gcatgatgat
     1681 gttggaacgg ttctctgatt tctttctcgt gttctatgca tggaagtgta tcaa
//
LOCUS       DASTWE010000888         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1413720, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000888 DASTWE010000000
VERSION     DASTWE010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413720"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..960
                     /locus_tag="VFT30_09255"
     CDS             253..960
                     /locus_tag="VFT30_09255"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU5092861.1"
                     /translation="MTIENRLAQLDWKVIEASLWQRGYAKTASLLTAKECEALVALYC
                     KDQLFRSRIDMKRFRFGEGEYKYFTYPLPPLVQALREQIYPRLAVIANAWVKALGQLD
                     LFPLSHDQLLAFCRRNGQIKPTPLLLSYGAGDYNCLHQDIYGAVAFPLQLTAFLSRPE
                     HDFTGGEFLLVEQRPRTQSRGEVLAPEQGELVIFATRHRPVQGSRGYYRVNMRHGVST
                     VRSGSRFTLGVIFHDAK"
BASE COUNT          249 a          281 c          281 g          244 t
ORIGIN      
        1 tagtcaatac tgtcagatgc caacgagcga agagcccgat gatctttcga tcgtcgggct
       61 ctttttctaa cctgagtgac atccaaaccg gggtctgccc agaacctgag acattaccaa
      121 cgccgacaca ctacatcggt acatcccctt ctgcgtcgtg tacgattgga tttttagaac
      181 gttgttgctg atttggtggt gatgattcat tgctaagaag tgtctgtcgg tgatacggaa
      241 gccaagacaa gcatgactat cgaaaataga ctggcccaac tagattggaa agtgattgaa
      301 gcatcattat ggcaacgggg ctatgccaaa actgcttcct tgcttacagc caaagagtgc
      361 gaggccctcg ttgccctgta ctgcaaggac caattgtttc gaagccggat cgacatgaag
      421 cgtttccggt ttggcgaggg ggagtacaaa tacttcacgt atccattacc gccgctggtc
      481 caagcgctgc gcgaacaaat ctatccccgc ctagcggtga ttgcgaacgc ctgggtaaag
      541 gcattggggc agctggatct ttttccgctg agccacgatc aattgctcgc cttctgtcgt
      601 cggaacggac agatcaagcc cacaccgttg ttactcagtt acggggctgg ggattataac
      661 tgccttcatc aggatatcta tggagcggtc gcgttcccgc tccagctcac cgccttcctc
      721 agtcggcccg aacacgattt taccggtgga gaattcctgc tcgtggaaca acgaccgcgc
      781 acacaatcac gcggcgaggt cttggccccc gagcagggtg aactcgtgat ctttgcgacg
      841 cgacaccgtc ccgtgcaagg aagccgaggc tattaccggg tcaacatgcg gcacggcgtg
      901 agcaccgttc gatccggttc acgctttact ctcggcgtga ttttccatga tgcaaagtaa
      961 tgaccgccat aagacacggt agatgggata atctgtaggc atgcggatag ccacatggaa
     1021 tgtcaactcg ctgaaggctc gcctggaaaa ggtca
//
LOCUS       DASTWE010000889         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1381068, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000889 DASTWE010000000
VERSION     DASTWE010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381068"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFT30_09260"
     CDS             <1..192
                     /locus_tag="VFT30_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092862.1"
                     /translation="KTFPPTVIVEKQMAETEIDCKVAQRIEAKKRRDFKRADEIRAEL
                     KSLGITLEDKPDGTTRWKR"
     gene            167..949
                     /gene="rlmB"
                     /locus_tag="VFT30_09265"
     CDS             167..949
                     /gene="rlmB"
                     /locus_tag="VFT30_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU5092863.1"
                     /translation="MAQPAGSANAQEILYGLHAVREALKAGNRPLQRVLVIRTDKQFT
                     DLVQLARSRRVPIHVQPLSSFDRLVPNGKHQGVVAFTSAKSYQTEESILARAAQRHEA
                     PLLVILDGVEDPHNLGAVLRTAEGAGAHGVFIPERRAAGLTSVVAKASAGAIDHIPVA
                     RVTNMGRSIESLKAAGVWIYGVTPSANKVFTDIDLREPVGLVLGGEGTGIRPGVLQHC
                     DDRVCIPLRGQVQSLNVSAAAAIVLFEAVRQRYPTRPDFTGS"
     gene            complement(936..1214)
                     /locus_tag="VFT30_09270"
     CDS             complement(936..1214)
                     /locus_tag="VFT30_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5092864.1"
                     /translation="MAKITGKSRPRKSLADLEPPPRTKRPESLTSREQEILELIWAGF
                     KNKEIGHRLKISVKTVEAHRANMMKKMRVSNTAQLLKTAIQGGALRIR"
     gene            1385..2167
                     /locus_tag="VFT30_09275"
     CDS             1385..2167
                     /locus_tag="VFT30_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU5092865.1"
                     /translation="MHEGGLLPLLIAGLFGALIGSFLNVCIYRLPRRESIAWPGSHCP
                     KCSHPIAWYDNVPILSYLALAGRCRHCAVPISFRYPLVEILNAAGYVSLLWFFGRSWV
                     AVAYGLLYSALLVVAGTDLSHKIIPNAVTFPGIVLGLVCATTILPLGFLDSLLGILIG
                     GGILWLLAWVSPYLFGKEGMGGGDIKLLAMIGAFLGWKPALMTIMVGSFLGSLVGVTL
                     IAVQVIKREDYIPFGPFLVCGALVALFFGQFILDWYQGLLAG"
     gene            2461..2889
                     /locus_tag="VFT30_09280"
     CDS             2461..2889
                     /locus_tag="VFT30_09280"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEU5092866.1"
                     /translation="MNQEGKTLTELMVVVAIIGMVATMASPNYSVFNSRSQIRCTTEE
                     IASELRLARQLAMTYRDRVRIVVDLEQQALTTQFVNTATIHHAYHYGGKGIVIEEPSA
                     GPEILFHPSGRSASPTTIQLHSLEGQTQQLTVSITGRVSL"
     gene            2898..>3104
                     /locus_tag="VFT30_09285"
     CDS             2898..>3104
                     /locus_tag="VFT30_09285"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5092867.1"
                     /translation="MKNDGFSLIEVMVAMVIAGVALMGTLGAVEVSSRHVQQSGLKSK
                     AMELAQARLEAKRAIRWQSLLEDDL"
BASE COUNT          700 a          780 c          853 g          771 t
ORIGIN      
        1 aaaacctttc caccgacggt aatagtagaa aaacaaatgg cggagacgga gatcgactgc
       61 aaagtggctc aacgcatcga ggcaaagaag agaagagatt tcaaaagagc tgacgaaatt
      121 cgtgccgaat taaaatcttt gggtatcacg ctagaggaca agcccgatgg cacaacccgc
      181 tggaagcgct aatgctcagg agattctcta cggtctccat gccgtacgcg aagcgctgaa
      241 agccggaaac cgaccattgc aacgtgtgct cgttattcgg accgacaaac agttcaccga
      301 tctggtgcaa ttggctcgat cacgccgtgt tccgattcat gttcagcctc tgagctcctt
      361 cgaccgtctg gttcccaacg gcaagcacca aggcgttgtg gcctttacct cagcaaagtc
      421 ctatcagacc gaagagtcga tcctcgctcg agctgcccaa cgccatgagg cgccactgtt
      481 ggtaatcttg gacggagtcg aagatccgca caacctcggc gccgtgcttc ggacggcgga
      541 gggcgcgggt gcccacggag tatttatccc cgaacgacgg gccgccggac ttacatccgt
      601 ggttgccaaa gcttcggccg gagcaatcga tcatattccc gttgcacgcg tgacaaacat
      661 gggcagatcg atcgagtcgt tgaaagcggc gggagtatgg atctatggcg tgacgccatc
      721 ggcgaataaa gtatttacgg acatcgatct aagagaaccg gttgggctgg ttctgggagg
      781 ggagggaacg ggaatcagac ctggtgttct gcaacattgc gatgaccgtg tctgcattcc
      841 tctgagaggc caggtccaat cgctgaacgt gtccgctgcg gcagccatag tgctgtttga
      901 ggcggttcgt cagagatatc cgactaggcc tgactttacc ggatcctgag cgcgccgccc
      961 tggatggcgg tcttcagcag ttgagccgta ttcgacaccc gcattttctt catcatattg
     1021 gcgcggtggg cttcgacggt tttgacgctg atcttcaacc ggtgaccgat ttccttattt
     1081 ttaaagcccg cccagatcaa ttccaaaatt tcctgttcgc gtgatgtgag cgactcaggc
     1141 cgttttgtac gcggaggagg ttcaagatcg gcaagagatt tccgaggccg tgactttcca
     1201 gttatcttcg ccatagtagt tggttctcca tatagcaggt gagtccatca ggtatactta
     1261 gatactcaag cgagtagtac gactaccaac agtgtagtca ttatggaatg atcatccaag
     1321 aatgtaaata aaggcacttc ggccctagta gggttactgg gtagggaaag aactctgagc
     1381 aattatgcat gaggggggac tgttaccgct actgattgcc ggccttttcg gtgcgctcat
     1441 cggtagcttc ctcaacgttt gtatctatcg tttaccacgg cgggaatcaa tcgcgtggcc
     1501 aggctctcat tgtccgaaat gctctcatcc catcgcctgg tacgataacg ttccgattct
     1561 gagttacctc gcgttggctg gacgctgcag gcattgtgct gtacccattt cgttccgcta
     1621 tcccctggtc gagattctga atgcagcagg ttatgtaagt ctcctttggt tcttcggccg
     1681 aagctgggtt gctgtggcgt atgggttgct ctattcagcc cttctcgtgg ttgccggaac
     1741 ggatctttct cataagatca tccccaatgc cgtgacgttt ccggggatcg ttctaggcct
     1801 cgtctgtgcg accaccatct tacccctcgg gttcctggac agcttgctgg gaatcctcat
     1861 cggcggtgga atcctgtggc ttttagcttg ggttagtccc tatctcttcg gaaaagaggg
     1921 gatgggaggg ggggacatca aacttctggc aatgataggg gcatttctgg gatggaaacc
     1981 tgcgctgatg acaatcatgg tcgggtcgtt ccttggatca ctcgtgggag tcacccttat
     2041 tgccgtacag gtgatcaagc gcgaggatta cattcccttc gggccgtttc tggtttgcgg
     2101 tgcgttggtg gcactattct ttggccaatt tattcttgat tggtatcaag ggctcttggc
     2161 cggataacgg ctctcctggc cgattcccac cctttcgtac tgtatctctt tctgtgagaa
     2221 gccgtatctt ctgaggtact actgctatcc acccgtccgc tcctttctta aagcgttaga
     2281 tctaccgggt cattccaagc atttctcgtt ttagattcat caattcaatg gcttaaggag
     2341 gtttcaaaca cccccttgct cattaaggag atatccatga gggcctcgac tatgagcggc
     2401 acgagtcttg acatagcgac ggactaccta aatcagtgca gccagagata gaggaaaaat
     2461 atgaaccaag aaggtaagac gttaacggaa ctcatggtgg tcgtagcgat cataggaatg
     2521 gtcgccacga tggcatctcc caactactcg gtctttaact cgcgtagtca gattcgttgt
     2581 acgaccgaag aaatcgcttc ggaactacgc cttgcacgac agcttgcgat gacctatcgg
     2641 gatcgtgttc gaatcgtcgt cgacctggaa cagcaggccc ttaccactca gttcgtgaac
     2701 actgcgacta tacatcacgc gtatcactat gggggaaagg ggatcgttat cgaagagcca
     2761 agcgcaggac ctgagatcct tttccatcca agcggacgct ctgccagtcc aacgacgatt
     2821 caacttcaca gcctagaagg acagacccaa caactcaccg tgagtatcac gggtcgggtg
     2881 tcgctatgag gcaagtgatg aagaatgatg gtttcagtct gattgaagtg atggtggcaa
     2941 tggtgattgc aggagttgcg ctaatgggca cccttggtgc ggtggaggtt tcttccaggc
     3001 acgtacaaca gagcggtttg aaaagtaagg cgatggagtt ggcgcaggca aggttggaag
     3061 cgaagcgggc cattcgttgg cagtccctcc tggaagatga tctc
//
LOCUS       DASTWE010000890         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1219081, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000890 DASTWE010000000
VERSION     DASTWE010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219081"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFT30_09290"
     CDS             complement(<1..1193)
                     /locus_tag="VFT30_09290"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5092868.1"
                     /translation="MPDDGPRQEGQSSGIDGLAWQEGWSAYTKKAWPEAQRFFEKIVK
                     EHPESSLVPSAKAFLVERLLRDDVSGQTRSEAILEYKKLLRDHPQSSNARRAEWRIAD
                     LYFEQEAFQEAQALYEQAMAHSVNIPFDGNRALLGLGYTFMAMGRWSEAERAFADVRK
                     RSDDEQLLQGATLGLAHALFRQQRFSEAQPIYDISYRRWPHLLRGDPLSLQRYAVTQV
                     KLHHEAPARELLLLFYNLYPRHEYAPTALLQVAEGLRAGTQQPLAEFVYALISSLYPD
                     STSDTTAILRLAALRAEKMLPAGSNSLGLTVSAMIHNVPVPDQTDASYRSLLEEIATR
                     EVGNPMGSEALYYLGKGYEHASDMNRALRTYKEITLRAVDKNDPWAMKATERLSVLLT
                     PWIEAA"
BASE COUNT          266 a          370 c          356 g          297 t
ORIGIN      
        1 gccgcctcga tccacggtgt cagaagaacg gacagccgct ctgtggcttt catcgcccat
       61 ggatcgttct tatcgacggc tcgcagtgtg atctctttgt aggttcgaag cgctcgattc
      121 atgtcgcttg catgttcata tcctttgccg aggtaataca gggcctcgct ccccatcggg
      181 ttaccgacct ctcgagtcgc aatctcttcc agcagcgatc ggtaggaggc atcggtctgg
      241 tccggaaccg ggacattgtg gatcatggcg ctgaccgtga gcccgagcga attgctccca
      301 gccggcaaca tcttctccgc acgaagtgca gcaagtcgga gtatggccgt cgtatccgac
      361 gtgctgtccg gatagagcga tgaaatcaga gcgtagacaa actcagcgag cggctgttga
      421 gtacccgccc gcaacccctc ggccacctgc agcagagccg tcggcgcata ttcatggcgg
      481 ggatagagat tgtaaaagag cagcaggagt tctcgagcgg gagcctcatg atgcagtttc
      541 acctgcgtga cggcataccg ctgaagagat aacggatcgc ctctgagaag atgcggccac
      601 cgtcgatagc tgatatcgta aatcggctgc gcctcagaaa atcgttgctg tctgaacagc
      661 gcatgggcca gccccaacgt cgctccttgg agcaactgct cgtcgtcact tcgctttcgc
      721 acatcggcaa acgcacgctc ggcctcgctc catcttccca tggccatgaa cgtatagcca
      781 aggcccagca aagcccgatt gccatcgaag ggaatattga cggaatgggc catggcttgt
      841 tcgtaaagcg cttgtgcctc ttggaacgcc tcctgctcga aatacaaatc cgcgatccgc
      901 cattctgctc tcctggcgtt tgacgattgt ggatgatccc gaaggagttt tttgtactcc
      961 agaattgcct cggatcgggt ttgccccgag acatcatccc gcaacaagcg ctccaccaag
     1021 aaggctttcg ccgatgggac gagcgagctt tcaggatgtt cctttacgat cttctcaaag
     1081 aagcgttgcg cctccggcca tgctttcttt gtgtatgcag accagccttc ttgccaagcc
     1141 agtccgtcaa tccctgaaga ttggccttct tggcgaggcc cgtcatccgg caaaggtcgt
     1201 acaagtcgtt ctcctagtat cggattcctt atggagatcg ctcgttcgga tgctgctgcg
     1261 atcgaggtat tgggagaagg atttggggg
//
LOCUS       DASTWE010000891         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1187103, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000891 DASTWE010000000
VERSION     DASTWE010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187103"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFT30_09295"
     CDS             <1..631
                     /locus_tag="VFT30_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092869.1"
                     /translation="SEHPIVRQERIKIVAERLGISEARLIERYPALLAQPKRGAEASR
                     TQPEQGIPLNALFKGLPEERDLLLLLLHGKLSSADVRRLRPESFTVEPCRKLVEIALT
                     HIDKDGRMNLRSVLDASMGEPDCSALATELSMREDHFDDVQAHITDCLNCLDRKQSEQ
                     AMRELIVKLKTAEREGRMDEARMLNMQINQVRMRKAGTPTAGVVSLVKE"
     gene            634..>1850
                     /locus_tag="VFT30_09300"
     CDS             634..>1850
                     /locus_tag="VFT30_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEU5092870.1"
                     /translation="MPKQELLGEVKKLITIGKEKGFLTYDELNSTLPAEVVSSDQFGS
                     IMAMFGEMDIEIIEASDGDRVQKRSEGEVGEDVEEVESDSEEENDKAIDLTPGALSRT
                     DDPVRLYLKEMGSVALLSREGEIEIAKRIEEGKKDIASVIYGMPMTIEFVLALRDQLK
                     NAQIDVREIVPVQETEEDFEEDQPPVERDYEELRVKTLESLNAVRKVSLALKTLAEKG
                     KHLGSDPVKQKKFKKQFDAIRQQVVNKIESVNLHGVLKDRMVQRVRDLAVQIRAAERE
                     AVSCQRRIGVGGEAGAELLRKMCRSRHDFLAVKRKTGVSEEALVEIRKVYQTAKAKIR
                     QLETEEALVPAEEIKDAVKHLDLAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFL
                     DLIQEGNIGLMKAV"
BASE COUNT          458 a          436 c          607 g          349 t
ORIGIN      
        1 gagcgagcat ccgatcgtgc ggcaggagcg catcaaaatc gtggcggaac ggctggggat
       61 cagcgaagct cggctgattg aacgctatcc ggcgcttctg gctcagccga aacgaggcgc
      121 ggaagcgtcg cgcacgcagc ctgaacaggg aattccgctc aatgctctgt tcaaagggct
      181 ccctgaggag cgggatctcc tcttacttct gctgcacgga aaattgtcgt ctgccgacgt
      241 ccggcgcctg cggccggagt cctttaccgt cgagccctgc cgcaagctcg tcgagattgc
      301 gctcacccat atagataaag atgggcgcat gaatctcaga tcggtcttgg acgcgtcgat
      361 gggcgaacct gactgtagtg cattggcgac ggaattgtcg atgcgcgagg atcatttcga
      421 cgatgtgcag gcgcacatta cggactgttt gaattgtctg gaccgaaagc agtcagagca
      481 ggccatgaga gagcttattg tcaaactgaa aacggctgag cgcgaaggtc gtatggacga
      541 ggcgcgcatg ctgaatatgc agatcaatca ggtacggatg cggaaagccg gcacgccgac
      601 cgccggtgtg gtttcattgg tgaaggagta gtcatgccga aacaagagtt gctcggtgag
      661 gtgaagaaac tgatcacgat cgggaaggaa aaaggttttc tgacgtatga cgagctgaac
      721 agcaccttgc ctgctgaagt cgtatcttca gatcagtttg gcagcattat ggcgatgttc
      781 ggtgaaatgg atatcgaaat catcgaagcc agcgatgggg atcgggttca gaaacgatcc
      841 gaaggcgaag tcggagaaga tgtggaggag gtcgagtcgg attccgagga ggaaaacgat
      901 aaggcgatcg atctgacgcc gggcgcgctc agtcgcaccg acgaccccgt gcggctctac
      961 ctcaaggaaa tggggagtgt ggctctcctc agccgcgaag gcgagatcga aatcgcgaaa
     1021 cgcattgaag aggggaaaaa ggatatcgct tcggtgatct acggcatgcc gatgaccatc
     1081 gaattcgtcc tggctctccg cgatcagctc aagaacgccc agatcgacgt acgcgagatc
     1141 gtgccggtcc aggagaccga agaggacttt gaggaagacc agccaccggt ggagcgggat
     1201 tacgaggagt tgcgggtcaa gacgctcgag tcgttgaatg ccgtgcgtaa ggtctcactc
     1261 gcgctgaaga cgttggccga gaaaggcaag catctgggca gtgatccggt caagcaaaag
     1321 aagttcaaga agcagttcga tgcgatccgc cagcaagtcg tgaacaaaat tgaatcggtc
     1381 aacctccatg gcgtgctgaa ggaccgaatg gtgcagcgtg tccgcgatct cgcggtgcag
     1441 atccgggccg ccgagcggga agcggtcagc tgccaacggc ggatcggtgt ggggggggag
     1501 gccggtgccg aactgctgag gaaaatgtgc cggagtcgtc atgatttctt ggcggtcaag
     1561 cggaagaccg gcgtgtcgga agaagccctg gtggagattc gaaaggtcta tcaaaccgcc
     1621 aaagcaaaga ttcgccagtt ggaaacggag gaggcacttg ttccggctga agagatcaag
     1681 gatgcggtca agcatctcga tctggccgaa gaaaaggtaa agcgcgggaa ggctgaattg
     1741 gttgaggcga atttgcgact cgtggtcagc atcgccaaga aatatacgaa ccgaggtctc
     1801 cagttcctcg atttgattca ggaaggcaat atcggattga tgaaggcggt
//
LOCUS       DASTWE010000892         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_540918, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000892 DASTWE010000000
VERSION     DASTWE010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540918"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2988
                     /locus_tag="VFT30_09305"
     CDS             <1..2988
                     /locus_tag="VFT30_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092871.1"
                     /translation="QTLQGEARRIEVTLLTREFRVLRQALQEAETEVLNLDQQESEKA
                     AEQARLTTELEQARLDAIATADSIGKIREELAAVEQQQAHALTAAEVERNRGLLFGQQ
                     QVQESAELEELVRSQENLATALQTIESSLTAVEEEMGIRDRALGELEEEMTHLLHQRA
                     AAVAEEERGRKDVLQLAVLVANTEQGISQLTKRMEDLAGRGTRLSSERDELRSQREAA
                     IDRHEGLRKEYGEADRLVSQLRTEQRTAQEEAAQATGLLQSLDHVILRRSEELAGVDS
                     RLEALQSVVREEMGYGRQGTQQGPALKSCDGVQDAVAEWLVIPSGMERAVEAVLGERV
                     RGWFVDEPSVGRRLIRFLQEEALGRGSFIPQRPRWEGGRAHDWWTSLATQPGVVGLAV
                     DLVQTDAARTATRELLFDRVVIVRSLDQAMQLWEQHMWSAPNGPILVTLDGEVVDASG
                     IVTGGQVQGTPGLLERRREVVDLEAKRHSLVTELDQSKQQRDMVFAQIQSLTHQERQL
                     GDALRDAEMQNLSLRKDEEKLQHVLNDLDHRLTGMEAEIQEGISEGQRLEQESLSVQG
                     QLGQWLAEKNGQETMLGRVRERLGLLDQNMRTHQERVTEAKLTAEGLRAKREHEQLNR
                     VRVTQQIQEAEDRRRALGEHLNGLKGLIEQSQTEQARQEVLCQEHGVTAGRVKAALVA
                     AQERQAQQVAASQTLESGLEGVRRGISVIRDARMTVEVRRAEIRTQLGTIEGTLSGTY
                     QLDPLLLNSNDSASEADAAAVQETEHRSDVELKEQLQKLRDRLDRMGPINLAAISEHQ
                     ELEERHKFLSIQEQDLSNSIASLKEIIQRIQRTTKEMFAATYEELQQKFTEVFGQFFP
                     GGRAELLLVDEPPPENGEPVGNQEPGIEIVAQPPGKRLKSITMLSGGEKTLTAMALLF
                     ASFLIRPTPFCLLDEIDAPLDEENIGRFTGVLKELAQNAQFLVITHNKRTMSIADSLF
                     GVTMEEPGVSKLVSVRLGDLQPA"
     gene            3557..>3747
                     /locus_tag="VFT30_09310"
     CDS             3557..>3747
                     /locus_tag="VFT30_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092872.1"
                     /translation="MALSQIPDRQSLPQSIANLALARVTLEALLMPASQAEEERLRVT
                     RDERQEELDDDPLGPARGIV"
BASE COUNT          888 a          890 c         1211 g          758 t
ORIGIN      
        1 cagacgttgc agggggaagc gcgcagaatc gaagtgacct tgttgaccag ggaattcaga
       61 gtattgcgac aggccttgca ggaggcggag actgaagtgc tgaacctgga tcaacaagaa
      121 tccgaaaaag cggcggaaca ggctcgactc acgacggaac tcgaacaggc gcgtcttgat
      181 gcgattgcga ccgccgattc cataggaaag attcgagagg aactggcggc ggtcgaacaa
      241 caacaggccc atgctctgac ggcggcggag gtcgaacgaa accgagggtt gttgttcggc
      301 caacaacagg tacaggaatc agctgagctg gaagaactgg tcagatcaca ggagaatctg
      361 gccaccgcgc ttcaaactat cgagtcgtca ttgacggcgg tcgaggagga gatggggatt
      421 cgggatcggg ctctcgggga actcgaagag gagatgacgc acttgctcca ccagcgggct
      481 gcggctgtcg cggaggaaga gcgagggcgc aaagacgtcc tgcaactggc tgttcttgtc
      541 gcgaataccg aacaaggtat ctcgcaattg accaagcgaa tggaagatct tgcgggccgc
      601 ggaactcggt tgtcgtcgga gcgagacgaa ctgcggagcc aacgtgaagc ggcgatcgat
      661 cgccatgagg gcttgcgcaa ggaatatgga gaggcggatc gccttgtttc gcaactacgg
      721 acggaacaac gaaccgcgca agaagaggcc gctcaagcta ctggccttct ccagtcgttg
      781 gatcacgtga tcctaagacg ctcggaagag ctggccggcg tggattcgcg tcttgaggcg
      841 ttgcagagtg tggttcgtga ggagatgggg tacggtcggc aagggaccca gcaaggcccc
      901 gccttgaagt cgtgtgacgg agtgcaggat gctgtggccg aatggctggt gattccgtcg
      961 gggatggaac gggcggtaga ggccgtgttg ggggagcgtg ttcgcggatg gtttgtggat
     1021 gaaccgtccg tcgggcggcg gttgatccgt tttctccaag aggaggcgct gggacggggc
     1081 agttttattc cgcagcgccc gcgatgggaa ggcggacgag ctcatgactg gtggacgtcg
     1141 ctagcaacgc agccaggcgt tgtcgggctt gctgtggatc tggtccaaac tgacgccgcc
     1201 cgaacagcga ctcgcgaatt gctgttcgat cgtgttgtca ttgttcgatc attggaccag
     1261 gcgatgcagt tatgggagca acatatgtgg agtgctccaa acggtccgat cctggtgacc
     1321 ctggatggtg aagtggtgga tgcatctggc atcgtgacgg gtggtcaggt tcaaggcacg
     1381 ccgggtttgc tcgaacgccg acgagaggtg gtcgatcttg aagccaaacg tcactcgctc
     1441 gtcacggagc ttgatcagag caagcaacag cgagacatgg tgtttgccca gatccagtcg
     1501 ctcacccacc aggaacgcca actcggagat gcgcttcgtg acgccgaaat gcagaatctg
     1561 tcgctgcgca aggatgaaga aaaacttcag catgtgctga acgatcttga tcatcgactc
     1621 accgggatgg aggcggaaat tcaggaaggt atcagtgaag gtcagcggtt ggaacaagaa
     1681 tcgctctcag tccagggcca attgggtcag tggcttgcgg agaagaacgg gcaagaaacg
     1741 atgttgggga gagtgcgcga gagactgggg ttgctcgatc aaaatatgag aacgcatcaa
     1801 gaacgtgtga cggaggcgaa gctgactgct gaagggttgc gagctaaacg ggaacatgag
     1861 caactgaatc gggttcgggt gacacaacag attcaggagg cggaagatcg gcgtcgtgca
     1921 ttaggtgagc acttgaacgg tctgaagggg ctcattgagc agagccaaac agaacaagct
     1981 cgtcaggaag tcctctgcca agaacatggc gtaaccgcag ggcgggtgaa ggcagcattg
     2041 gtcgccgctc aggagcgaca ggcgcaacag gtggcggcga gccaaacgct tgaatccggt
     2101 ctggaggggg tgcgccgggg gatttcggtc attcgggatg ctcggatgac ggttgaggtt
     2161 cgccgagcgg aaattcgcac gcaattgggg actatagaag gaacattgtc ggggacctat
     2221 cagcttgatc ctctcttgct gaattcgaac gactcggcga gcgaggctga cgccgcagcc
     2281 gtacaggaga ccgagcaccg ttcggacgtc gagttaaaag agcagttgca gaagctccgt
     2341 gaccggctgg atcgtatggg gccgatcaac ttagccgcga tcagcgaaca tcaggagctg
     2401 gaggagcgcc acaaattttt gtctatccag gagcaggacc tgtcgaactc gattgcttcg
     2461 cttaaggaga ttattcagag gattcagcgg acgacgaagg agatgttcgc ggccacgtac
     2521 gaggaattgc agcagaagtt caccgaggtc ttcggccaat tctttcctgg cgggcgggcc
     2581 gagctactgt tggtcgatga accacctccg gaaaacggcg aaccagtcgg caatcaggaa
     2641 ccgggaattg aaattgtggc acaaccaccc ggcaaacggc tgaagagcat caccatgctt
     2701 tcgggcggag aaaagacgct caccgcaatg gcgctgttgt ttgcgagctt tttgatcagg
     2761 ccgacaccgt tctgtctgtt ggacgaaatc gacgccccgt tggacgagga aaacatcggc
     2821 aggtttactg gagttttgaa ggagctggcg cagaacgcgc agtttctcgt catcacccac
     2881 aataagcgga cgatgagcat tgccgattcc ctctttgggg tcactatgga agagcctggg
     2941 gtgtccaaac tggtatccgt caggctcggc gatctacagc cggcctagga ataggccgcc
     3001 ttcagaattc aatgatgacg aacttctagg atccattaga acctatgagt ccaggcttcg
     3061 acgagattct atccagcgtt acacgctctt ggtaacgcaa tgatggggag ttcccatgcc
     3121 tttctccact gctttagtta gactggtttc ggaacgggtt ggccgtcggc cgtgataggc
     3181 cgccagtcgt gatggacaaa gtttttcgca taccgaaata gcctcttttt ttctcagcgg
     3241 tgccaattcg cgtagggagt ttaatccatt actgtgcaaa agtggcctgc ttccatgccg
     3301 aaaaatgttc caacgttggg agagagccgt tgagtagagc agcgaactgt tcatttattt
     3361 attggagtgg gatgttccta gagaaaggaa gcgatattca tcaatgactt gtgtccctgt
     3421 gtcgtttgct cccaatcggg gatatgcggt ctagaaaggt ggttttgcta tgagaaacga
     3481 atgacccacg gtatcgttgt tgctctacaa gagtgcgaag accgctgctt cacaacatgt
     3541 acaatgagga gcgatcatgg cgctttcaca aatcccggat agacaatcac ttccacaatc
     3601 gatcgccaat ctcgcccttg cacgagtgac cttggaagcg ctgctcatgc ccgccagtca
     3661 agctgaggag gagcggttgc gggtgacgcg agacgagcgg caggaggagc tagatgatga
     3721 tccgttgggg cctgccagag gtattgt
//
LOCUS       DASTWE010000893         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1381286, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000893 DASTWE010000000
VERSION     DASTWE010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381286"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1053)
                     /gene="bamA"
                     /locus_tag="VFT30_09315"
     CDS             complement(<1..>1053)
                     /gene="bamA"
                     /locus_tag="VFT30_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU5092873.1"
                     /translation="PFITEIVFDGNEELSDDKLKEKITIKRQAFLDQQQAKESAEKIR
                     LAYQEDGYFSAQVIPIVQTLDADRKRLTFFIKEGEKAKVKTVNFEGMRAASKSEIFKV
                     TATREWVPWYGLVTQLKLPSFLSDAGVLKREEMNNDVERIREVLLNKGYLNAQVGLPS
                     VELSDDKKWFVVTYAVVEGEPFTVAEIGFRGNTVFEDPELRDKMKIKPGEIFQRQKIR
                     DEIGRLTDLYGSKGYAFAEVIPNVNPNAEERTATIILNIKEGEMMRIRQINVHGNDKT
                     KDNVIRRELRVDEQDVIDTPSLKRSFQRLNNLNFFETVEILPQQVDADKVDLNVRVKE
                     KPTGQFSIGGGFSTLD"
BASE COUNT          198 a          299 c          265 g          291 t
ORIGIN      
        1 tgtccaaggt actgaatccg ccgccgatac tgaactgccc tgtcggtttt tcttttaccc
       61 tgacgttcaa gtccaccttg tccgcgtcca cctgctgcgg caggatttcg accgtttcga
      121 agaagttgag attattcagg cgctgaaagc ttcgcttgag cgacggcgtg tcgatgacgt
      181 cctgttcatc gacgcgcaac tcgcggcgga tgacgttgtc tttggtcttg tcgttgccat
      241 gaacattgat ctgccggatg cgcatcatct cgccttcttt aatattgaga atgatggtcg
      301 cggtgcgttc ttcggcattc ggattcacgt tgggaatgac ctccgcgaag gcgtagcctt
      361 tgctgccgta gagatcggtc agtcggccga tttcatcgcg gattttctgc cgctgaaaga
      421 tttctcccgg cttgatcttc attttgtccc gtaactccgg gtcttcgaaa accgtattgc
      481 cgcggaaccc gatctcagcc acggtgaacg gctctccctc gacgacggca taggtgacga
      541 cgaaccattt tttatcgtca ctcaactcca ctgagggtaa gccaacctgg gcgttcagat
      601 accctttgtt gagcaacacc tccctgatcc gctcgacgtc attgttcatt tcctcgcgct
      661 tcaggacgcc ggcatcggag agaaaggagg gcagcttaag ctgcgtcacc aggccatacc
      721 agggtaccca ctcccgcgtc gcggtcacct tgaatatttc ggacttggac gcggcccgca
      781 tcccctcgaa gtttaccgtc ttgaccttgg ctttctcgcc ttccttgatg aaaaaggtga
      841 gccgcttacg atcggcatcc aaggtctgaa cgatcgggat gacttgggcg ctgaaatagc
      901 cgtcttcttg ataggcaagg cgaatttttt ccgcgctctc cttcgcctgc tgctgatcca
      961 aaaatgcctg tcttttgatc gtgatctttt ccttcagctt gtcgtcgctt aattcctcat
     1021 tcccatcgaa gacgatttcg gtaatgaagg gtt
//
LOCUS       DASTWE010000894         1403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1187364, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000894 DASTWE010000000
VERSION     DASTWE010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187364"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1403)
                     /locus_tag="VFT30_09320"
     CDS             complement(<1..>1403)
                     /locus_tag="VFT30_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5092874.1"
                     /translation="TTLIGNLPGFVYRCRNDHGWIFEYLSEGVSELTGYTVEEYLIHR
                     TTSYGQNIHPGDRERVRHEVQAAFEEHRPFELTYRILTKCGEVKWVWERGEGIYAQDG
                     TRSYLEGFVTDVTERKRAEHLLRQSEERYRRLIAVSPYAILVDRGDRVIFANDQAIKL
                     FGAVKAEEILEKSPLDLFHADDRHIVRERIHELFEGRTQAPMLEEKIATLDGRAVDVE
                     VSRARFVDEEGPAILIMLRDISERKRLQEQLRRTERVAELGTLASGMAHEIGTPMNVI
                     LGRAEYLMDRVTEESIKRGLQTIITQVERITRVMNQLLSFARRKAPQRIPLDLRKVIE
                     DGMEMFQERLARNQIRVEMGMVDPCPLVLADADQMSQVLLNLIMNALHAMPEGGTLRV
                     DLEPDQPMVKLTITDTGHGIPPEVIKKIYDPFFTTKEFGKGTGLGLTVVKGIIDEHQG
                     SIAVESEEGNGTRFTVHL"
BASE COUNT          302 a          422 c          334 g          345 t
ORIGIN      
        1 aggtgaacgg taaacctggt tccgttccct tcctcgcttt ctacggcaat ggagccttga
       61 tgctcatcaa tgattccctt caccacagtc aatcccaagc ccgtcccttt cccgaactcc
      121 tttgtcgtaa agaacggatc gtagatcttc ttgatgacct ccggtggaat tccgtgacca
      181 gtatcggtga tggtgagttt caccatcggt tgatccggct ccaagtcgac acgaagagtg
      241 ccaccttccg gcatggcatg aagggcgttc atgatgaggt tgagcaagac ttggctcatc
      301 tgatccgcat cggccagcac caaggggcac ggatcgacca tccccatttc gacccggatc
      361 tgatttcttg cgagacgctc ctgaaacatc tccataccgt cctcaatcac ttttcttagg
      421 tcaagtggaa tgcgctgcgg agccttgcgt cgcgcgaatg aaagcagctg attcatcacc
      481 ctcgtaatcc gctcaacctg cgtgatgatc gtctggaggc ccctttttat cgactcctcc
      541 gtcacacgat ccatcaaata ttcagcccgg ccaagaatca cgttcatcgg cgtcccgatt
      601 tcatgcgcca tgccggaggc cagcgtcccc agttcagcga cccgctcagt cctacgcaat
      661 tgttcttgaa gccgcttccg ttcgctgata tcacgaagca tgatcaaaat agccggtccc
      721 tcttcgtcaa caaatcgagc tctactgact tctacatcaa cagcccttcc gtcgagcgtc
      781 gcgattttct cctcgagcat gggagcttgc gttctgcctt caaacagttc atggattcgc
      841 tctcgaacga tgtgacgatc atcggcatga aacaagtcta ggggtgactt ttcgaggatc
      901 tcctcggcct tcaccgcgcc gaacaacttg atcgcctggt cattggcaaa gatgacgcgg
      961 tcccctcgat ccactaaaat cgcataaggc gaaacggcga tcaggcgccg gtaacgttct
     1021 tcgctttgcc ggagcaagtg ttcagcacgc ttgcgttcgg tcacgtcggt cacgaaacct
     1081 tccaggtaac tgcgggtgcc gtcctgcgca tagatcccct cccctctctc ccagacccat
     1141 ttgacctcgc cgcatttcgt aagaattcgg taggtcaact cgaaagggcg atgctcctca
     1201 aacgcggcct gaacttcatg ccggacacgc tcgcggtcac ctggatggat attttgacca
     1261 taagaggtcg ttcgatgtat gaggtattct tctacggtgt aaccggtcag ctccgacact
     1321 ccttcgctga ggtattcaaa tatccagcca tggtcatttc ggcaacgata cacgaatccc
     1381 gggagattgc cgattaaggt ggt
//
LOCUS       DASTWE010000895         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_88622, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000895 DASTWE010000000
VERSION     DASTWE010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88622"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..665)
                     /locus_tag="VFT30_09325"
     CDS             complement(312..665)
                     /locus_tag="VFT30_09325"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEU5092875.1"
                     /translation="MISAIHTALSGLAAFSKQIEVVAHNVANVNTDGFKKSKTEFVEI
                     PNGGVLPVVEKDDSSGPTVLRDTGSNQTMVELSNVDLGEEVVQQIVAQRSFEANLQTL
                     RRGDALLGSILDIKK"
     regulatory      complement(751..845)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1138..>2297
                     /locus_tag="VFT30_09330"
     CDS             1138..>2297
                     /locus_tag="VFT30_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5092876.1"
                     /translation="MTVPATPISDHPSNGSPTWLLTREDIALILEATTDIIVVTDQVG
                     RIVFLNPIAQQLLRRTRDAVGVTFHDIIGCLTSKTDGTTLCPFSQMMSTGEVAWIPSH
                     SWKRGDGTQFDLSISFWPRTQRGVRVGGVIVVRDMTDALEFQRDVQRAARLAEDAPNP
                     IVEFDATGAMLYANTGMLNLMTQCGLLEAGVEVMFPANLSAMLHECLMTDSSLSRVEH
                     VAANRVIAWSFFPLKELELVRAYGLDITSDVALRRAKEAAEESARAKGIFLATMSHEL
                     RTPMNGVLGCTQLLKDTSLTDQQRQLIETMHRSAEALLTLVNDILDFSKIEAGKMTLE
                     VADVNLRALICDITTLAEGLASSKGLTVSVNIDADVPEEFRGDPIRLRQILF"
BASE COUNT          547 a          566 c          639 g          545 t
ORIGIN      
        1 atctcgatgc ccaagtcggc ttgctctgtc tcgattccac gggactgaag aaaccctgtt
       61 aaataggcgg tggatgggta gggagtgttc agctgagtca agggaggaat taccaataag
      121 acatgaggac aaatcatgac ctcgacatag catatggatc ggcgggaaag ctatgtctca
      181 gaagatgaaa aatcggccaa tctgcaggaa agggatgggc tacgagacaa ttggtctcaa
      241 cagatagcca gcgtttctgg gtatgctacc gagtggcatt tgacgcgtgt gacgaacacg
      301 gcggtggtca actacttctt tatatccagg atactgccga gtaaggcatc tccccttcgt
      361 aaggtctgga gattcgcttc aaagctacgc tgagccacga tctgctgcac cacttcctcg
      421 cccaaatcaa cgtttgagag ctcgaccatg gtctgattgc taccagtgtc gcgaagaacg
      481 gtcgggccgg atgagtcgtc tttctcaaca acagggagga caccaccatt agggatttcg
      541 acgaattctg tttttgattt tttgaacccg tcggtattga cgttggcgac attgtgcgca
      601 acgacctcga tctgtttgct gaaggcagcc aagcccgaga gtgctgtatg gatagcggaa
      661 atcatgcggc acctccattc ggttgaggcg aattgagagg aaggtaccga ggaaggggaa
      721 ggaaagcctg actggtcaga tcatcgattg tgtggtagct aggctgaccc tggaacatgt
      781 tccgtccctg ttaaggtccc tcggctttgc gccccaccct ctcgggtggt ttgccctttt
      841 tcacctttgc ccgaatctct atcggttgtc tgacgtaaga cttgagcatg cgattggttg
      901 gagtgtcgca ggagaccgat ctgtgacaac cgcgaacgac cgtcatgaac cggcagaatc
      961 gagcacatgc ggtgcagttg ggttgtttgc acgcgaccgt catggcatac gctggatcgc
     1021 ttgcggcatg attctgccag tttctcaatt aaaaaatacc cgtaggtaca ccgataaggt
     1081 atgactaata gcccgaactc tttgtgccct atagatagca gtggtacaga tcagcctatg
     1141 acggttccag ccacgcccat ctccgatcat ccttccaatg gctcgccgac ttggttgctg
     1201 acccgagagg atatcgctct cattctcgaa gctacgacag atatcatcgt tgttacggat
     1261 caagtaggcc gtattgtctt tctgaatcct atagcacaac aactcctccg aaggacgcgt
     1321 gatgccgtcg gagtgacatt tcacgacatt ataggatgtc tgacatcgaa gacggatggg
     1381 acgaccttat gcccatttag ccagatgatg agcaccggcg aagttgcttg gataccctcg
     1441 cattcttgga aacgaggaga cggcactcag tttgatctct cgatttcgtt ctggccacga
     1501 acgcaacggg gagtacgagt cggcggcgtg atcgtggtgc gcgacatgac cgatgccctg
     1561 gagttccaga gagatgtgca gcgcgcggca cggctggcag aagatgcgcc aaatccaata
     1621 gtggagtttg acgcaacggg agccatgctt tatgccaaca ccggcatgct caatttaatg
     1681 actcaatgcg gactactcga agcgggggtc gaagtgatgt tcccggcaaa tctatcggcg
     1741 atgctacacg aatgtctcat gaccgattct tcgctgtccc gggtggaaca tgtcgcggcc
     1801 aatcgggtta tcgcctggtc gttctttcct ctgaaagagt tggaattagt tcgtgcatat
     1861 ggactggata ttacctcaga tgtggcctta cgccgcgcga aagaagcagc tgaggagtcg
     1921 gctcgggcga agggaatctt tctcgccacg atgagccatg agcttcgtac tccgatgaac
     1981 ggagtactgg gatgcacgca actccttaag gacacatcgc tgaccgacca acagcggcag
     2041 ctgatcgaaa ccatgcatcg ctcggcagag gcgttgttga cgctggtgaa cgacattctc
     2101 gatttctcaa aaattgaggc cggcaagatg actctcgaag tggctgatgt caatctgcgg
     2161 gcactgatct gcgacataac gacattggcg gaggggttag cttcctcaaa aggtctcact
     2221 gtttcggtga acattgatgc cgatgttccg gaagagttca gaggtgatcc gatcaggttg
     2281 cgacaaatcc tattcaa
//
LOCUS       DASTWE010000896         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_961289, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000896 DASTWE010000000
VERSION     DASTWE010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961289"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1069
                     /locus_tag="VFT30_09335"
     CDS             <1..>1069
                     /locus_tag="VFT30_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEU5092877.1"
                     /translation="LKLLNKFIITKHFKMETLASVMESLRPQEYTASIDLEDAYLHIP
                     MHPDVFHLFRFEVAGVVYQFEVLPFGIATAPHLFTKMMRPILGELHRQGAAAHAYIDD
                     WLLRDASPDVLVDNVRKTFLIFKDLGVGVNQPKSRLEPARQFQFLGATLDLDAFTAKP
                     SPERVANIVEAIESLAPSPMMSARALCQIIGQLDSAAALVPMGRWRVRPFHWFRHRNW
                     IRNHGYEVLLVTSLIPRNSLRWWADEENLLQGVPVHDPQPELHLFTDASMEGWGGYVG
                     AHVASGLWSNSETELHINVLELRAVFLSLERLLPFVTDHVVAVMSDNTTTVAYPRRGG
                     GTHSHSLHQEVSRIFELCLEWK"
BASE COUNT          231 a          292 c          306 g          240 t
ORIGIN      
        1 ctaaaactgc tgaacaaatt catcatcacc aagcacttca agatggagac tttggcatca
       61 gtcatggagt cactgcgtcc tcaagagtat acggcgtcca tcgacttgga ggacgcatac
      121 ctgcatatac ccatgcatcc ggacgtgttt catctgttcc gtttcgaagt cgctggcgtg
      181 gtctaccagt tcgaggtgct tccgttcggt atagccacgg cgccgcacct gttcaccaag
      241 atgatgcggc cgattttggg agaacttcat cgacaggggg cggcggcgca tgcttacatc
      301 gacgactggt tactgcggga cgcttcgccg gatgtgctgg tagacaacgt aagaaagacg
      361 tttctgatct tcaaggactt aggcgtggga gtaaaccaac ccaagtctcg cctggagccg
      421 gccagacagt ttcagttcct tggggcgaca ctggatttgg acgcattcac ggcaaaaccg
      481 tctccggagc gggtggccaa cattgtggaa gctatcgagt cgttggcccc gtcaccgatg
      541 atgtcagcaa gggcactttg ccagattatc ggccaattgg attcggccgc cgcactggtt
      601 cccatgggcc ggtggcgggt gcgcccgttt cattggttcc gtcacaggaa ttggatacgc
      661 aaccacggat acgaagtcct gctcgttaca agtctcatac cgcggaacag cctccggtgg
      721 tgggccgacg aggagaatct tcttcaggga gtaccggttc acgaccccca gccagaactc
      781 cacctcttca ccgacgcgtc gatggaaggt tggggtggtt acgtcggagc ccatgtggct
      841 tccggtctat ggtcaaacag cgagacggag ctacatatca atgtcctgga actgcgagcg
      901 gtcttcttat cactggaacg gttgctccca tttgtcaccg atcacgtagt agcagtaatg
      961 agcgacaaca cgacaacggt cgcttatccc cgcagaggcg gggggaccca ctcgcactct
     1021 cttcatcagg aggtttccag gatcttcgag ttgtgcctgg agtggaaga
//
LOCUS       DASTWE010000897         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1543012, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000897 DASTWE010000000
VERSION     DASTWE010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543012"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1000
                     /locus_tag="VFT30_09340"
     CDS             <1..>1000
                     /locus_tag="VFT30_09340"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5092878.1"
                     /translation="QVKNVVGPSVQIDEQGIVSAAWVEEDKDRRTILFARSDRPDGPL
                     GLPVPVNQPGENVYYRQESPALTVHGNEVFISWAQTHPKITPDKPFSGELRLSTSTDG
                     GRTFAPSILVNDDGQVIQHTFDSIQAAPNGTLHMVWIDGREGKKEPGTFVARSVDHGR
                     TIAKNFKIDDETCVCCRTSVATSADGVIYVAWRKIFDGNIRETVVSRSTDDGQTFSSS
                     VVVGNDRWVYPACPHRPASLGVDRSGRLYVVWYTEGADETPAVYVAYSDDQGKSFSPK
                     KQLNRAKGTFPDHPQMAVDPAGRLVVVWEEQSPVRREVVVSYSLDRGQSFSTPIKLN"
BASE COUNT          224 a          258 c          306 g          212 t
ORIGIN      
        1 caggtgaaga acgtagtcgg gccgtcggtg cagattgatg agcaaggtat cgtttcagcg
       61 gcttgggtag aagaggataa ggacaggcgc accatcctgt ttgcgaggtc ggacaggccg
      121 gatggaccgc tcggattgcc ggtccccgtc aaccagccgg gtgagaatgt ctactatcgt
      181 caagaatctc cggctttaac ggtccatggg aatgaggtct ttatctcctg ggcgcagacc
      241 cacccgaaga tcacccccga taagccgttt tccggggaac tgaggctcag cacgtccaca
      301 gacggaggac ggacgtttgc tccatctatc ttggtgaacg acgacgggca agttatccag
      361 catacgttcg actcgattca ggccgctccg aacggaacgc tgcacatggt ctggatcgat
      421 gggcgtgaag gcaaaaagga accaggaacg ttcgtggcca gatcggtcga tcatggccgc
      481 acgatcgcga agaatttcaa gatcgacgac gagacgtgcg tgtgctgcag gacatcagtg
      541 gcgacctctg ccgacggtgt gatctatgtt gcatggcgaa agattttcga cggcaatatt
      601 cgtgagactg tggtgtctcg atcgacggat gacggtcaga cattttcctc ttcggtcgtt
      661 gtcggcaatg atcgttgggt atatccagcc tgtccgcatc gcccggcatc cctcggtgta
      721 gatcgatcgg gccgtctgta tgtcgtgtgg tacacagaag gtgccgacga aacccccgcc
      781 gtgtatgtgg cctactcgga cgaccaagga aagagttttt ccccgaaaaa acaattgaat
      841 cgtgccaagg ggactttccc ggaccatccg cagatggcgg tcgatccggc gggacgactc
      901 gtggtggtgt gggaggaaca atcgccggtt cgtcgagaag tggtggtcag ctattcactc
      961 gatcgtggcc aatcgttcag cacaccaatc aaactgaacg
//
LOCUS       DASTWE010000898         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_56380, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000898 DASTWE010000000
VERSION     DASTWE010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56380"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..999
                     /locus_tag="VFT30_09345"
     CDS             37..999
                     /locus_tag="VFT30_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5092879.1"
                     /translation="MTEIAEYFELVKDRYGLSSDYALAEKLGIAQPEANLMRRGLKIP
                     KPELCIKIAKHLNKNPVELLLIAQKDKAPKQAKEYWNLALTAIDVMLHVPKSPKYIPR
                     KVAAIGQELRQLETQTLLYEGAEANAEAVRLVQTAEESIDAIMERWNIWKKGEALYPS
                     YLLANQAAVKRGVVIRRLLVLTREQMQQESVIADAIQVMDDQHRAGIKIFFAFREDLD
                     RAAAFQRFEEDYRQQGAARDLNTAIFDREILIFSRSYGHVQLGIVGPSTPITMINQLE
                     ITWKPELLRDLDPAPLFDMTRYVFEYSGPRAFKMELERFTKATK"
     gene            996..>1394
                     /locus_tag="VFT30_09350"
     CDS             996..>1394
                     /locus_tag="VFT30_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEU5092880.1"
                     /translation="MKFPSEPFKIKVVEPIRRTTREERDKLLRSAGYNLFQVPAESVY
                     VDLLTDSGTAAMSDRQWAGLMLGDESYAGSRNYYHLEETVRSIFGYKHVIPTHQGRMA
                     ENLLFSTVVKPGMCIPNNIHFDTTRANVEHQ"
BASE COUNT          360 a          400 c          376 g          258 t
ORIGIN      
        1 gccgagacgt gaggatcacc ccctgatgag gtcagcatga cggaaatcgc ggaatacttc
       61 gaattggtca aggaccgcta tggattgagc agcgattacg ccttggcgga gaaactcggc
      121 atcgcacagc cagaagccaa cctcatgcga cgcggactca aaatcccgaa gccggagttg
      181 tgcatcaaga tcgccaaaca tttgaacaag aacccggtcg aactcctcct gatcgctcaa
      241 aaagacaagg ccccgaaaca ggccaaagaa tactggaact tggcactgac cgccatcgac
      301 gtcatgcttc atgtccccaa aagcccgaaa tacatcccac gtaaggtggc agccatcgga
      361 caggaactcc gccagcttga aacgcagact ttgctctatg aaggagccga agcgaatgcg
      421 gaagccgttc gactcgtcca gacagctgaa gagtccatcg acgcgatcat ggaacgatgg
      481 aacatctgga aaaagggaga ggcgttgtac ccaagctacc tcctggcgaa ccaggcggcg
      541 gtcaagcggg gtgtcgtgat ccgtcgcctc ttggtcctga cgcgcgagca aatgcagcaa
      601 gagtcggtga tcgcggatgc catccaagtc atggacgacc aacatcgggc cggcatcaag
      661 atcttctttg cattccgtga agatcttgat cgagcagccg cctttcagcg tttcgaggaa
      721 gactacaggc agcagggggc tgcgcgggat ctcaacactg cgattttcga ccgggaaatt
      781 ctcatttttt cccgatccta cggccacgtg cagctcggaa tagtgggtcc cagcacaccc
      841 attacaatga tcaatcaact ggaaatcacc tggaagccgg agttgctgcg ggaccttgac
      901 ccggctccgc tcttcgacat gacccgatac gtcttcgaat attccggccc acgggctttc
      961 aagatggaat tggaacgatt cacgaaagcg accaaatgaa attcccgtcg gaaccgttca
     1021 agatcaaagt ggtggagccc attcgtcgca cgacccgcga agagcgtgac aagctgctgc
     1081 gcagcgccgg ctacaatctg tttcaggtcc cggccgaaag tgtctacgtc gatctcttaa
     1141 ccgatagcgg cacggcagcc atgagcgacc ggcaatgggc gggcctgatg ttaggggatg
     1201 agtcctatgc gggcagcaga aactactacc atctggaaga aacagtccgg tcgatcttcg
     1261 gctacaagca cgtcattccg acgcatcaag ggcgtatggc cgagaatctc ctcttctcca
     1321 cggtcgtcaa gccaggcatg tgcatcccca acaacatcca cttcgacacg acaagggcca
     1381 acgttgagca tcag
//
LOCUS       DASTWE010000899         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1543030, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000899 DASTWE010000000
VERSION     DASTWE010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543030"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..>1191
                     /locus_tag="VFT30_09355"
     CDS             146..>1191
                     /locus_tag="VFT30_09355"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5092881.1"
                     /translation="MQELDTPTFRLAVAQFDQAAETMGLDPNLRERLKLPQRSLVVSL
                     PVRMDDGHVEVFTGYRVQHDSSRGPSKGGVRYHPDVNLGEVAALAMWMTWKCALAGLP
                     YGGAKGGVTVAPKQLSPAELQRLTRRYAAEIFPLIGPDKDVPAPDVGTDAQVMAWMMD
                     TYSQQVGYAVPGVVTGKPLSIGGSLGREEATGRGVVYVTQEVLRHLKLSIENVTVAIQ
                     GFGNVGSHTARIMQQQGVRVIAVSDVHGGIYNPKGLDVMELLRRDPSQPVHDTKLGDA
                     ITNEELLRLECTILVPAALSEQITSKNADSLRCRILSEGANGPTTLEADRILADKGVF
                     VIPDILANSGGVIVS"
BASE COUNT          272 a          328 c          346 g          245 t
ORIGIN      
        1 ggcagtacgc cgaacagccg tcccagttga tgtcgtcaag cacgaactcg ctcaaatctt
       61 cgcgacggtg tttgccctca attggtcaac tggtgcatga cacagcagcg tacgtaactg
      121 actggtcttt gcgtagagga gtcctatgca ggaacttgat acaccgacct ttcgcctggc
      181 cgtggcgcag ttcgatcaag cagcggaaac gatgggactt gacccaaacc tccgcgaacg
      241 tctgaaactt ccacagcgat ctctcgtcgt cagccttcca gttcggatgg acgacgggca
      301 cgtggaggtt tttaccggct accgcgttca acatgattca tcacggggcc cctccaaggg
      361 aggcgtacgg taccatcccg acgtgaatct cggtgaggtg gcggctcttg ccatgtggat
      421 gacatggaaa tgcgcgttgg ccggcttgcc gtatggagga gcgaaaggcg gagtcacagt
      481 agccccaaaa caactgtcac ccgccgagtt acagcgcctg acacgacggt acgccgcaga
      541 gattttccca ttgattggcc cggacaagga cgtacccgcc ccagacgtgg gaaccgatgc
      601 tcaggttatg gcatggatga tggacacgta cagccagcaa gtcggctacg ccgttcctgg
      661 agtggtgaca ggtaaaccgc tctcgatcgg agggagcttg ggccgtgaag aagcaacggg
      721 acgcggagtc gtctatgtga cgcaagaggt gcttcgtcac ctcaaattgt cgattgaaaa
      781 tgtcacggtg gcgattcaag gatttggaaa tgtcggttca cacaccgctc gcatcatgca
      841 gcaacaaggt gttcgcgtca ttgccgtcag cgatgtgcat ggtgggattt acaaccccaa
      901 gggcttggat gtcatggagt tgcttcgtcg cgatccaagc caaccggtgc atgacaccaa
      961 gctaggggat gcgatcacaa atgaggaact cctgcgactg gagtgcacca ttctcgtccc
     1021 cgctgctctc tcagaacaga tcacaagcaa aaatgcagat tcactgaggt gtcggattct
     1081 gtcagaaggg gcgaacgggc cgacgacgct ggaggccgac cgcatccttg cagacaaagg
     1141 cgtctttgta atccccgata ttctcgccaa ttccggcggc gtcatcgtct c
//
LOCUS       DASTWE010000900         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1575271, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000900 DASTWE010000000
VERSION     DASTWE010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575271"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFT30_09360"
     CDS             <1..556
                     /locus_tag="VFT30_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5092882.1"
                     /translation="KDKIAIPNAGSEYLQAGVSVIRASVGDEQTKKFLEGLRDNAGTQ
                     VYQKSSQIVDAVAKGQVAMGVVNHYYVYRHLATQPTAPLAVLMPDQQEGGMGAIMNVA
                     GIGILKHTPRVENAKLLVEFLVAQAGQKMFADLDKEYPLHPEVKADSALVERKSFRAA
                     LVPLTKLAELREPTLLLIEQVGMR"
     gene            572..2155
                     /locus_tag="VFT30_09365"
     CDS             572..2155
                     /locus_tag="VFT30_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5092883.1"
                     /translation="MIGVRRSSVSPLQLTALASAALIVLPLGYVTILALSADLAVWHR
                     LWTTRVPELLWNTVSLAGVVALLTLVLGVSTAWMVTRFEFPGRRLWEVGLVLPLAMPT
                     YVLAYVYNYLLGFGGPVEQLWQMIAGPQARIVSPQSFWGVTLVMALDTFPFVYLLTRS
                     ALLSFNVSFEEVARTCGASPLRRMLFVTLPLLRPSIVAGVALVILYVVSDFGAVSLLR
                     YQTLTYAVFQQMTGRSDNQAASILSILLVILALLFLLTERWFRRKSRFYQTTGRFRTP
                     QRVRCGWVNTAALTTCLATVIGLAFGIPAYLLVTWSLSPEALAILDVRFFGFVWNSVL
                     LSTLAATAGVFVGLPLAYLASRKPTWLNTGCLQAAYAGYVLPGPVAALAVLVLFLNVL
                     PLLYGTVIVLIVAYVLHFLPAGLQSLEPSIQQITPNLEEASRTLGSTVQDTWRRVTLP
                     LIRNGVIVAWVLIFLQTMKELPATLLLRPVGFDTLAIRVWLEASEEYYQLAAPSALLI
                     VLVGLPALVLLLSRDWRAA"
     gene            2164..3282
                     /locus_tag="VFT30_09370"
     CDS             2164..3282
                     /locus_tag="VFT30_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5092884.1"
                     /translation="MNTPSHITQVDADRLEGQVHLYTPASSILELRSVSCAYDPSKPA
                     IHDISFSGREGEILCLLGPSGCGKTTILRAIAGLEPVRSGQIFLSGRLVSSSSETIPT
                     EERRVGMVFQEYALFPHLRVADNIAFGLGHLSRAERKCRVQEMLRLTGLEGLDRRYPH
                     ELSGGQQQRVALSRALVQNPVLLLLDEPFSNLDPDMAGRMRQEVHTLLRRMKTTTILV
                     THDHDEAFAMADRIAVLNQGVLEQMDSPELIYHLPATRFVADFVGQADFILGRIRQGM
                     VHTELGEFPDTMNSAEGSTVVVMIRPDDIHLMPNKSAEPRIVSRQFRGSENLYTIRLP
                     SGQVVHSSESSTSVYQEGTAVELRVSATHTVLFPAERS"
     gene            3415..3846
                     /gene="exbB"
                     /locus_tag="VFT30_09375"
     CDS             3415..3846
                     /gene="exbB"
                     /locus_tag="VFT30_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEU5092885.1"
                     /translation="MDALKDAIDYGVIGLLLVLSVWSVAIAIERWLFYRRVNLSQFSN
                     AQLLEITLTKHLVIIGTVAANAPYIGLLGTVLGIMLTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAFGLLVAIPCVVMNNVLRRRVSEILTEYKAQYGTEH"
     gene            3830..4204
                     /locus_tag="VFT30_09380"
     CDS             3830..4204
                     /locus_tag="VFT30_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU5092886.1"
                     /translation="MERNIDQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EVGDRKDVPIVISLTADGSLFLNDVPVPNGSLHSALNSRPRESAVVVRADKVTLLEKF
                     VSLVDEIRGLGFQQVSLEVIRL"
     gene            4204..>4695
                     /locus_tag="VFT30_09385"
     CDS             4204..>4695
                     /locus_tag="VFT30_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092887.1"
                     /translation="MDNSVTLTDNRTGEMAASWFVSCLLHGGLAVAAIFFVQRMHLAP
                     QTEPFQWNVAMVATLSPSMGSTPSIASMVPPTPALRQSTQPTTHAHPTVQPVPPRMTM
                     PVTSSHPEPIVEFTPQPNQAQRLHTESHSQDLTSSVPADSPPQAPSQHLSAPLALAEP
                     VPPA"
BASE COUNT          917 a         1411 c         1252 g         1115 t
ORIGIN      
        1 gaaggacaag atcgccattc ccaacgccgg aagtgaatat ttgcaagcag gcgtatcggt
       61 gatccgcgca agtgtgggcg acgaacaaac caagaagttt cttgagggtc ttcgggacaa
      121 cgccggcaca caggtctacc aaaaaagttc gcagatcgtc gacgccgtcg ccaagggtca
      181 ggtcgccatg ggcgtcgtga atcactatta cgtctatcgg catctcgcca cacaaccgac
      241 cgctccgctg gcggttttga tgcccgatca acaggaaggg ggcatgggcg ccatcatgaa
      301 cgtggcgggc atcggcatcc tgaagcacac tccccgcgtg gagaacgcca aattgctcgt
      361 ggaatttctc gtggcacaag ccggccagaa gatgttcgcc gacctcgaca aagagtaccc
      421 gctccatccc gaagtgaaag ccgactcagc tcttgtcgag cggaagagtt tccgggcggc
      481 cttagtacct ttgaccaagc tcgcagagct gcgagagcct accttgctgc ttatcgaaca
      541 agtagggatg cggtaacgct gcgggcgccc tgtgattggc gtacgtcgat catctgtttc
      601 ccccctccaa ctcaccgccc ttgccagcgc ggcgctaatc gttctgcccc ttggctatgt
      661 caccatcctc gcgttgtcgg ccgatcttgc ggtttggcat cgtctctgga cgactcgtgt
      721 ccccgaactg ctatggaaca cggtttcctt ggccggtgtc gtggcattgc tcacactcgt
      781 actcggcgta tcaaccgcct ggatggtgac tcgcttcgaa ttccccggcc gccgactgtg
      841 ggaagtcggt cttgtgctgc cgctggccat gccaacatac gtcttggcct atgtgtacaa
      901 ctatctcttg ggattcggcg gtccggtgga gcagctctgg cagatgatcg caggacctca
      961 agccaggatc gtctcacccc aaagtttctg gggagtcacc ttggtgatgg cactcgacac
     1021 gttccctttc gtctacttgc tcacacggag tgcgctcctg agttttaacg tgtcgtttga
     1081 ggaagtggct cgtacatgtg gagcgtcacc acttcgtaga atgctgttcg tcacccttcc
     1141 gttgctgcgt ccgtccatcg tcgccggcgt cgccctcgtc atcctgtacg tcgtctcaga
     1201 cttcggcgcc gtctcgctgc tgcgctacca aactctgact tacgccgtgt ttcagcaaat
     1261 gaccggacgg tccgacaacc aggccgcgag tatcctgagc atcttgttgg tcattctggc
     1321 actcctgttc ttattgaccg agcgctggtt ccgtcgaaag agccggtttt accagaccac
     1381 cgggcgcttt cgaaccccac aacgagtccg gtgcggatgg gtgaatactg cggctctgac
     1441 aacatgcttg gccaccgtga tcggcttggc cttcggaatt cctgcctatc tgctggtgac
     1501 atggagcctg tcacctgagg ccctggcgat tctcgacgtc cgcttcttcg gcttcgtctg
     1561 gaacagtgtc ttgctgtcga ccctggccgc cacggccggt gtattcgtcg gactgcccct
     1621 cgcctatctt gccagccgaa aaccgacctg gctcaatacc ggttgtttgc aagccgccta
     1681 tgccggctac gtcttgccag gaccggtcgc tgcgctcgca gtgttggtcc tctttctaaa
     1741 cgtcctaccg ttgttgtatg gtacagtaat cgtattgatc gtcgcctatg tcctacattt
     1801 tcttccagct ggcctccaat cactcgaacc gtcgattcaa caaatcacgc ctaatcttga
     1861 agaagcttcc cgcaccctgg gttctaccgt tcaggatacc tggcgtcgcg tgacgctgcc
     1921 gctcattcgc aacggggtca tcgtcgcgtg ggttctgatc tttttgcaga caatgaagga
     1981 gcttccggca acgttattat tgcgacccgt gggctttgac accttggcga tccgtgtttg
     2041 gctggaagca agcgaggagt attaccaact ggcggctccg tccgccttat tgatcgtgct
     2101 cgtcggctta ccggcgctgg tcttgttgct gtcccgtgat tggcgagcag cataaaaccc
     2161 accatgaaca caccgtcgca catcacgcag gtggatgccg atcggctcga aggtcaagtc
     2221 cacctctaca ccccggcctc ttctatcttg gaactccgct cagtgtcctg tgcctatgat
     2281 ccaagcaagc cggcgatcca cgatatttca ttttccgggc gggaaggcga aatactctgt
     2341 ttgctcggac cgtccggctg tggcaagacc acgatcttgc gagccatcgc cggtttggaa
     2401 ccggttcggt ccggacaaat atttttatcg gggcggttgg tctcctcctc ctcggaaacg
     2461 attccgacgg aagaacgccg cgtcggtatg gtctttcagg agtatgctct tttcccacac
     2521 ctgcgcgtcg ccgataatat cgcctttggg cttggtcatc tgtcgcgggc tgaacggaaa
     2581 tgtcgtgtcc aagagatgct gcgactgacc ggcctcgaag ggctcgaccg acgctacccg
     2641 catgagttgt cgggaggaca gcagcaacga gtggcgctct cacgagcatt ggtacagaat
     2701 cctgtcttgc tcctcctgga tgagccgttc agcaatcttg accccgatat ggcaggtcgt
     2761 atgcggcagg aagttcacac cctcttacgc cggatgaaaa caacgaccat tttggtgacg
     2821 catgaccacg acgaagcctt cgccatggcg gaccgtatcg ccgtcctgaa tcagggagtg
     2881 ctcgagcaga tggattcccc ggagctcatt tatcatcttc ccgctacgcg gttcgtggcg
     2941 gattttgtgg ggcaagccga ctttatcctc ggccggattc gacaaggcat ggtgcatacc
     3001 gaactgggcg agttccccga taccatgaac agtgccgagg ggagcacggt cgtcgtcatg
     3061 atccgcccgg acgatatcca tctgatgccc aacaagtcgg ccgagcctcg cattgtgtcc
     3121 cgccaattcc gaggctcgga gaatctttac acaatccgcc ttccttctgg acaagtcgtc
     3181 cacagcagtg aaagctccac cagcgtctac caggaaggga cggccgtcga actccgtgtt
     3241 tccgccaccc atacggtgct ctttcccgcc gaacggtctt gacaactcta gacaggcaga
     3301 ttagaccgaa tgcatgcgca gattgacagc tttcacggaa tctggcatgg aggcacaggt
     3361 tccctaattc cccgcccgac atgcaccacc tttttcatga tgtaaggagt cgcgatggac
     3421 gcgttgaaag acgccattga ttacggagtc atcggtctgt tactggtcct gagcgtatgg
     3481 tccgtggcta tcgccatcga acgctggctc ttttatcgac gtgtcaatct ttcacagttt
     3541 tccaacgcgc aactgctgga aatcactctg accaaacacc ttgtgattat cggcacggtg
     3601 gctgccaacg ccccgtacat cggtctcctc ggaacggttc ttggtatcat gctgacattt
     3661 catacgatgg gaacctcggg cacgatggcc gtgaacacca tcatgatcgg cttgagcctg
     3721 gcgttgaagg cgaccgcctt tggtcttttg gtcgccattc cttgtgtggt gatgaataac
     3781 gtgctgcgtc gtcgagtgag cgagatcctg accgagtata aggcacaata tggaacggaa
     3841 cattgaccag atcaacgtga tcccgctcgt ggacgtgatg ttggtcttgc tcgtcattgt
     3901 attgaccacc gccaccttca tcagtacagg gcaaattccc gtcaacctgg ccaaagccaa
     3961 agaggtgggc gatcggaagg atgtcccgat cgtgatttcc ttgaccgccg atggaagtct
     4021 attcttaaat gatgttcctg tccccaatgg aagccttcac tccgctctga attcacggcc
     4081 tcgtgaatcg gccgtggtcg tacgagccga caaggtgacc ttgctcgaaa aattcgtatc
     4141 gttagtcgac gaaatccgcg gcctagggtt tcaacaggtc agcctggagg tcatccgtct
     4201 gtgatggaca attccgtcac gcttacagac aatcgaacgg gcgaaatggc ggcgagttgg
     4261 ttcgtgtctt gccttctgca cggaggactg gccgttgccg cgatcttttt cgtacagcgc
     4321 atgcacttgg cgccgcagac tgagcccttc caatggaatg tggcgatggt ggccaccctt
     4381 tcgccatcaa tgggatcaac cccgtctatt gcctcgatgg ttccacctac gccggccctt
     4441 cgacagtcca ctcaaccaac gactcatgct caccctacag ttcagccggt tcccccgaga
     4501 atgaccatgc cggtcacttc atctcatccg gaaccgatcg tcgaatttac gccgcagcct
     4561 aaccaagcgc agcgtctcca caccgagtct cattcacaag atttgacgtc ttctgtaccg
     4621 gctgactcgc ctcctcaggc tccatcgcag catctttcag ctccattagc gctagctgag
     4681 ccggtcccgc ctgct
//
LOCUS       DASTWE010000901         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_476764, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000901 DASTWE010000000
VERSION     DASTWE010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476764"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VFT30_09390"
     CDS             <1..1337
                     /locus_tag="VFT30_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU5092888.1"
                     /translation="GLKIALDHLDAVITLIRGSQSPDEARAGLIRQFGLTEIQANAIL
                     DMRLQRLTQLERAKLIEEYQEVLKQIEQLKSVLASEALVRTIIKDELTEIREAYKDER
                     RTQIVKEEAEISLEDLIAEEEVIVTISHTGYIKRNAVSLYRAQRRGGKGKIGMGIKEE
                     DFAENLFTASTHDSLLFFTDAGKVYWLKVHEIPEASRAAKGKALVNLLALAGSEKVTA
                     TLPVKEFRDDRYIIMGTKKGIIKKTELSAFSNPRQGGIIALGLEGGDKLIGVQLTDGQ
                     REILLGTRQGITIRFKEEEVRPMGRTAYGVKGITLEEGNEVIGMETINPDSTTSILTV
                     TEGGYGKRTPVGEYRVQGRGGKGIISVKTTERNGLAVGFLQVRDGDEIMLIAAQGKVL
                     RCKVEDIREIGRNTQGVRILDLDGEGDRVVGVARLAEVVEREEAASEDNPEA"
     gene            1363..1974
                     /locus_tag="VFT30_09395"
     CDS             1363..1974
                     /locus_tag="VFT30_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092889.1"
                     /translation="MYRQVSPPMSPSDPTTKKPLDIVVKFALGVFVGSFALIWGGMYL
                     SRPDRSIPPYTVGSQSGHVVATDVPAGTKDDQIERLLERFRKVGHQTRDFGPMKIYPT
                     TPGDPGGRYTRIVIYVFDDHRRTDPEVLAKYMAGDVAVVNDYERSMRGYYQLKDQNEE
                     GGIGTIPKNGRVTSDTRILFKGLVTDPLPVEAEVEQGISISPL"
     gene            2457..>2673
                     /locus_tag="VFT30_09400"
     CDS             2457..>2673
                     /locus_tag="VFT30_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEU5092890.1"
                     /translation="MAKETEDHRKVVATNRKAYHDYFIEEKFEAGIVLKGTEVKSLRD
                     RRVNLQDSYATVKDGEVFLQHCHISPYS"
BASE COUNT          695 a          663 c          780 g          535 t
ORIGIN      
        1 aagggctcaa gatcgcgctc gaccatctcg atgccgtgat tacgctcatc cgcgggtcac
       61 aatcgccgga cgaagcgaga gccggtttga tacggcaatt tggcctcacc gaaatccagg
      121 ccaatgcgat tctggacatg cggctgcagc gcctgaccca gttggaacgc gccaaactga
      181 tcgaggaata tcaggaagtg ctcaagcaga ttgaacagtt gaaatccgtg ctggccagcg
      241 aggcattggt ccggaccatc atcaaagacg agctgacgga aattcgtgag gcctacaagg
      301 acgagcgacg gactcagatc gtcaaagaag aggcggagat cagcctcgaa gacctgatcg
      361 cggaagaaga agtgatcgtg accatttcgc acaccggata catcaaacgg aatgccgtgt
      421 ccctctatcg cgctcagcga cgaggcggca agggcaagat cggaatgggt atcaaggagg
      481 aggatttcgc cgagaatctc tttaccgcct ccacccatga ttcactcctc tttttcacgg
      541 atgcgggaaa ggtttattgg ttgaaggtcc atgaaatccc cgaggccagc cgggccgcga
      601 agggcaaagc ccttgtcaat ctgctcgccc tggcaggcag tgaaaaagtc accgcgacgt
      661 tgccggtcaa agagttcaga gatgaccggt atattataat gggcaccaag aagggcatca
      721 tcaagaagac ggaactgtcc gcgttcagca atccacggca aggcggaatc atcgcattgg
      781 gactcgaggg cggtgataag ctgatcggag tccaactgac cgatggacaa cgggagattc
      841 ttctcggaac acgacagggc attaccattc gattcaaaga agaagaagtg aggccgatgg
      901 gccggacggc gtatggagtc aaagggatca cgctcgaaga aggcaatgaa gtcatcggca
      961 tggaaaccat caaccccgat tctacgacct cgattttgac tgtcacggaa gggggctatg
     1021 gcaaacggac tcccgtgggc gaatatcgcg ttcaaggtcg tggggggaag ggcattatca
     1081 gtgtaaagac aacggagcga aatggcttgg cggtcggatt tctgcaagta cgcgatggcg
     1141 acgaaatcat gttgatcgcc gcgcagggga aagtgcttcg ttgcaaggtg gaggacatcc
     1201 gcgaaatcgg gcggaatacc caaggggttc gtattctcga cctcgatggt gaaggagatc
     1261 gagttgtagg cgtcgcgaga ctcgcggaag tggtcgaacg agaggaggcg gcttcggaag
     1321 ataacccgga ggcttaacct tactgggccg tgcagttgtt ccatgtatag gcaagtgtcc
     1381 cctcccatgt ctccctcaga tcccacaacc aagaaaccac tggatatcgt cgtcaaattt
     1441 gcgctcggcg tcttcgtggg gtccttcgcc ttgatctggg gaggcatgta tctcagccgt
     1501 cccgatcgat cgattcctcc ctatacagtc ggttctcagt cagggcatgt cgtggcgacg
     1561 gatgtccctg caggaaccaa agacgatcag atcgagaggt tactagaacg ttttcgaaaa
     1621 gtcggtcatc agacgcgcga ttttggtccc atgaagatct accccacaac gccgggtgat
     1681 ccaggaggcc ggtatacacg gatcgtgatc tatgtattcg acgaccaccg gaggacagat
     1741 ccagaagtac tggcaaaata catggccgga gatgtagcgg tcgttaacga ctatgaaaga
     1801 tcgatgcgcg gatactatca actaaaggat cagaatgaag aaggaggaat aggtaccatc
     1861 cccaagaatg gacgggttac cagcgacaca cggatcctct tcaagggact tgtgacggac
     1921 cccttgccgg tcgaggcgga agttgagcaa ggtatctcga tatcacctct ctaaactgct
     1981 cacggatgga tggatcttgg atcatggcgg tatgcgatat cagttcagcc caagatttct
     2041 ctgcctgagc cgacccgagg tcggtggcaa gacttgcagg ggaagcatcc gtttcacagg
     2101 gaatctctcc gacacgaaac gcaatgtctg tcacggactc cgttcctgac tccgcctgca
     2161 gcttagccaa gagtgctggt ttgaggaacg tcaactgttg taaccagacg ggattcctaa
     2221 cgatgagata gagcttcttg aagcgaatct gggccggcca ggtatgggac gccataggct
     2281 ccctcacgat gtcgcgccac cgacgttgta agcgaagttc cagcaacctc gattcgagac
     2341 caagccgctt ggaaagacct gagaggatat tgccgaacga atcgagtgtg ctaaggccgc
     2401 tcatgtggca tcataggcgg taggggagaa agagatcaag gtctcaggat tgtgtcatgg
     2461 ctaaagagac ggaagatcat cggaaggtcg tagctactaa tcggaaggcc taccacgatt
     2521 attttatcga agaaaagttc gaggccggga ttgttctcaa gggcaccgaa gtgaaatctc
     2581 ttcgcgacag gcgggtcaac ttacaagaca gttatgcgac cgtcaaagac ggtgaagtct
     2641 ttctccagca ctgccacatc agtccctata gtc
//
LOCUS       DASTWE010000902         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_476765, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000902 DASTWE010000000
VERSION     DASTWE010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476765"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..756
                     /locus_tag="VFT30_09405"
     CDS             40..756
                     /locus_tag="VFT30_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5092891.1"
                     /translation="MAKRFNIRMAGVGGQGVVTGSHILSTAVINAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKEDGVAL
                     INNDGPMNLHRDQAAELKKLRAKLYYFPATKISLEVAGMDLATNMALMGCIGAITGLT
                     SMAGLDQAVKDRFLGKGFVVSGGTAALDSVVERKFKKKQELIDKNVAVMRAGWNYAVD
                     HGWASADVKRVEEPVAAATA"
     gene            776..1099
                     /locus_tag="VFT30_09410"
     CDS             776..1099
                     /locus_tag="VFT30_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEU5092892.1"
                     /translation="MYLVADISVEICASKSCKLCTQYCPEANTIQYSEEMGKEQGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPKAALTDNITYGEKSTTAVLA
                     SPVVG"
     gene            1121..>1317
                     /locus_tag="VFT30_09415"
     CDS             1121..>1317
                     /locus_tag="VFT30_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate:ferredoxin oxidoreductase"
                     /protein_id="HEU5092893.1"
                     /translation="MATTQDTRERIIVPGPAGFHPPSAAQLGVSLPDPGQGLFYGLLE
                     PNEEVVIEEMARKMLTSPNAT"
BASE COUNT          330 a          309 c          400 g          278 t
ORIGIN      
        1 attcccggca aagcctagag gttcacgagg aggtcaatca tggcaaagag attcaatatt
       61 cggatggcag gcgtcggggg gcagggtgtc gtcacgggtt cgcacattct gagcaccgcc
      121 gtcatcaatg ccggcggtga aagcaccatt gtgccgttct acgggtcgga aaagcggatg
      181 gcacctgtcg aaagctatgt ccgtgtttcg gatgagccaa tttacgaaat cggtgaaatc
      241 acgtttccgc acatcatcat catcttccat ccacaagtca tcacccacgg caagtcctat
      301 acaatgccgt tctatttcgg cttgaaagaa gatggggttg ccctgatcaa caacgacggg
      361 ccgatgaatc ttcacagaga tcaggcggct gagctgaaga agcttcgggc gaagctctat
      421 tattttccag ccacgaagat ctcattggaa gtggcgggta tggatctcgc cactaacatg
      481 gcgctgatgg gctgtatcgg cgcgattaca gggctgacga gcatggctgg attggatcag
      541 gccgtgaagg atcggtttct cggaaaaggc ttcgtggtgt caggcggcac cgccgccctc
      601 gacagtgtcg ttgagcggaa gttcaagaag aaacaggaac tgatcgataa aaacgtggcc
      661 gtcatgcggg ctggatggaa ctatgccgtc gatcatggtt gggcatcggc tgatgtcaaa
      721 cgggtggaag agccagtggc agcggccacc gcgtagccgg tcacaaagga gttttatgta
      781 cctcgtagcc gatatcagcg ttgaaatctg tgcctcgaag agctgtaagc tctgtacgca
      841 atattgcccg gaggccaata ctattcagta cagcgaggag atgggcaagg aacaggggtt
      901 caagtatggc tcagcctatg tcgcggtgga ccgttgtaaa gggtgtgcgc agtgtgtatg
      961 ggtctgtgac aacatggcca agaacaacgc catcaagatg atcatgatcg atcaattgcc
     1021 gaaggcggca ttgacggaca atattacata cggcgaaaag agcacgacgg ccgtcttggc
     1081 cagtccggtc gtcgggtaag agcgaggaaa ggggaagggc gtggcaacaa cacaggatac
     1141 tagagagcgc attatcgtac ccggacccgc gggtttccat cctccgtcag ccgcacaatt
     1201 gggtgtgtcg ctgccagatc ccgggcaggg gctattttat gggttacttg aacctaatga
     1261 agaagtcgtt atcgaagaga tggcgcgtaa gatgctgact agtccaaacg cgacaat
//
LOCUS       DASTWE010000903         1179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_250619, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000903 DASTWE010000000
VERSION     DASTWE010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250619"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFT30_09420"
     CDS             <1..255
                     /locus_tag="VFT30_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092894.1"
                     /translation="YAVGILLFTVILLFVVRPLLAMLSSTTAQTQAEASLSTLPGAVG
                     MAEASLAGGPDRAQIIDMARKNPDTTAVVVKQWLKNPHSA"
     gene            258..>1179
                     /gene="fliG"
                     /locus_tag="VFT30_09425"
     CDS             258..>1179
                     /gene="fliG"
                     /locus_tag="VFT30_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEU5092895.1"
                     /translation="MAKNLTGEQKAAILLRAIGEEAAAQVMKQLDPKEIKRLGSFMNG
                     TAQISRDEEDAVISDFRTASASGEVQFQGKEFIKTVLVKALGPEKAARIIESMTRKSY
                     PGLEALKWVDVKTLVQMLKVEHAQTVAVVLAHLESDQAGQVLAGLPESMRGDVALRLA
                     TMEEVQPDILEELSQSMQETLLASKGMGSQSIGGPEVMADILTRMDKANEGGIMVKIA
                     ERSQPLADAIRALMFVFDDLVKVDDRGVQELMKEISKDDLPLALRGANPEVKEKFFKN
                     MSSRAADMLKEDMEAKGPVKVSDVEKAQQNI"
BASE COUNT          298 a          278 c          369 g          234 t
ORIGIN      
        1 tatgctgtcg gaatcctttt gtttacggtg attcttctgt ttgttgtccg tccgttgctg
       61 gccatgttga gttcgacgac ggcgcagacg caagccgaag catcgctctc tacgttacct
      121 ggcgcggtcg gaatggcaga ggcctccttg gcgggcgggc cggaccgggc gcagatcatc
      181 gacatggcaa gaaagaatcc cgacacgacc gccgttgtcg tgaagcaatg gctcaagaat
      241 ccccatagcg cgtagcaatg gcgaagaatc tgacgggtga acagaaggcg gccattcttc
      301 ttcgtgccat cggagaagaa gcagcagccc aggtgatgaa acaactcgac cccaaagaaa
      361 ttaagcggct gggaagtttc atgaacggca ccgcccaaat ttcacgagat gaggaagatg
      421 cggtcatctc ggattttcgg actgccagcg cgtccggaga ggttcagttt caggggaaag
      481 aattcatcaa gaccgtgctc gtcaaggcgt tgggcccgga aaaagcggcg cgaatcatcg
      541 aatcgatgac ccgcaaaagt tatccaggac tggaggcact gaaatgggtc gatgtgaaaa
      601 cgctcgttca aatgttgaag gtcgagcatg cgcaaacggt ggcggtcgtt ctcgcacatc
      661 ttgaaagtga tcaggccggg caagtgctcg ccgggcttcc ggaatcgatg cgcggcgacg
      721 tggcgcttcg gcttgccacc atggaagagg tccagccgga tatcttggaa gaactgagcc
      781 aaagtatgca agagacgttg ttggcgagta aaggaatggg ttcacagagt atcggagggc
      841 ctgaggtcat ggccgacatt ctgacacgaa tggataaggc caatgagggc ggcattatgg
      901 tcaagattgc ggagaggagt cagccgttgg cggacgccat ccgggcgctc atgttcgtct
      961 ttgacgacct ggtgaaggtc gacgatcgcg gtgtgcagga attgatgaaa gaaatcagca
     1021 aagacgacct gccgttggct ctccgcggcg ccaatccaga agtgaaggag aaattcttca
     1081 aaaacatgtc gagtcgtgcc gcggacatgt tgaaagaaga tatggaggcc aaaggacctg
     1141 tcaaggtatc ggatgtcgag aaagcccagc agaatatct
//
LOCUS       DASTWE010000904         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_444298, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000904 DASTWE010000000
VERSION     DASTWE010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444298"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /gene="ureG"
                     /locus_tag="VFT30_09430"
     CDS             <1..456
                     /gene="ureG"
                     /locus_tag="VFT30_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEU5092896.1"
                     /translation="ALPVDRILGVETGGCPHTAIREDASHNQEAIDDLLRRHSDVELI
                     FLESGGDNLAATFSPELVDHVIYVIDVAGGDKIPRKGGPGITRSDFLVINKMDLAPHV
                     RADLSVMDWDTRRMRGDLPFAFTNILSGEGLAAVVAWVEQRIPQQARRS"
     gene            495..>1025
                     /locus_tag="VFT30_09435"
     CDS             495..>1025
                     /locus_tag="VFT30_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEU5092897.1"
                     /translation="MRRGTAEKRKTSEERSPSRKRRASPTFSTEFIGRVGELRLDYAK
                     PDRQTIIAHSYFTTPWKLLPPIYLDDTGAAYTLLVNPSGGLVGGDHLSIDMNLDRDAH
                     VLISSPSANRVYRTESKISQQHINITVGSGAILEWFPEHTIPFAGSRFRQILHATLAS
                     GATLLLWDAVASGRIAR"
BASE COUNT          222 a          286 c          285 g          232 t
ORIGIN      
        1 gccttgccgg tcgatcggat tttgggtgtg gagacgggtg ggtgtcccca cacggcgatt
       61 cgggaagatg cttcgcataa ccaagaagcg attgacgatc tacttcgtcg ccattcggac
      121 gttgaattga tctttctgga aagcggaggt gacaacttgg ccgcgacctt tagccccgag
      181 cttgtagatc acgtcatcta tgtgattgat gtggcagggg gtgacaagat cccgagaaaa
      241 ggcggtcccg gtatcactcg atcggatttc ctcgtcatca acaagatgga tttggcgccg
      301 cacgtgcgcg ccgatctgtc cgtcatggat tgggataccc gccggatgcg cggcgatctt
      361 cccttcgcat tcaccaatat cctttccggc gaagggctgg ctgccgttgt cgcgtgggtg
      421 gagcagcgca taccccagca agcgaggcgc tcataatcgt gcgggcttca acgggaccac
      481 ggaaaaagac tgctttgcgg agaggtacgg ctgagaaacg aaaaacttct gaagaaagaa
      541 gtccatccag aaaaagaagg gcatctccca cattttccac cgaatttatc ggacgggtcg
      601 gtgagctccg gcttgactat gccaagccgg accgccaaac catcatcgca cactcgtact
      661 tcacgactcc ctggaagctg ctccctccca tttatcttga cgacacggga gccgcgtaca
      721 cgcttctcgt caatccatct ggaggcctgg tcggagggga ccatttgtcc atagacatga
      781 acttggatcg agacgcacac gtcctgattt cctcgccttc cgccaatcgg gtctatcgaa
      841 ccgagagcaa gatctcacaa cagcatatca atattaccgt tgggagtggt gcgatcttgg
      901 aatggttccc tgaacatacg atcccttttg ccggttcgcg atttcggcag atacttcatg
      961 ccacactggc atcgggtgcg acgcttctct tgtgggatgc cgtcgcatcc gggcgtatcg
     1021 cccgc
//
LOCUS       DASTWE010000905         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1381576, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000905 DASTWE010000000
VERSION     DASTWE010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381576"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(765..>2727)
                     /locus_tag="VFT30_09440"
     CDS             complement(765..>2727)
                     /locus_tag="VFT30_09440"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU5092898.1"
                     /translation="ADQSAAEDTAFSFTVPSGAFADDDAAHGDLLTYNAVLADGSPLP
                     NWLSFNPTTRTFNGTPGPGDAGVLRLAVTATDTQEVGITDQFTLTVSGPLPQTVVGTT
                     GDDELMGGRGDDTLAGGAGNDALSGGSGDDTYFFNLGDGVDTVHDTSLPGEGNTIQFG
                     TGIMPDDLSLGIGSFIIRVGTNGDALHLTPFDPNNALGAHSIETFRFADGTTLNYSQL
                     VARGFDLTGTTGNDSVNGTNVVDRIRGLAGNDTLNGGSGDDVLDGGTGTDTMRGEVGD
                     DTYIIDSSSDLVIEAANEGTDTAQSSMTYTLGNNVEHLTLTGTSAINGTGNTLNNIMI
                     GNSANNTLTGGAGNDRLDGEFGNDTMVGGIGNDTYVVSQTGDVVTESSNQGIDTIESN
                     LTYMLGGNVENLTLTGAAAINGTGNSANNVLVGNIGNNTLDGGSGNDMADGGAGDDHL
                     LGSSGNDQLLGGLGNDALNGGSGSDVLYGGDGIDTLDGGSGDDQLVGGAGNDTLTGGS
                     GADQFASGAGNDTMTGGSGNDVYNLLRGDGQDTIIDSDPFSSNQDRAIFDATINPLDL
                     VISRQANNLRLVIHGSSDQVTVENWYLDTTNRIETIQAGNGQTLLSTQVDQLIQAMAS
                     FSRQSGLTWDQAIDQRPQDVQAVLAASWQ"
BASE COUNT          596 a          815 c          633 g          683 t
ORIGIN      
        1 aagctgcctg gcccgcatcg tcatgagaga tttaagggcc tcttgccgac gctgaacctc
       61 ttcttcctca gccatgatgc cacgaatatc ggttcctaac ataggtcccc acctccttca
      121 acgcgatgaa acttgtcagc cattataccg atttcatgcc tgatctttaa ataggtcata
      181 atatattgat ttcattaaat aaatatcatt tttatcgtgc gatttattga agctcacgct
      241 tccattgtgt atcatatatc gcatgtcgat agggctcttt atccagaact ggaggctcta
      301 caaaaaccaa tccatcgaat cgttatcaca cgcggcaaga attcccactg tactcctaga
      361 acagatcgag gcggatcaaa cggagcccac caccgttata attgaagctc ttgcttccgc
      421 gcttcgcatt cctccctctt ggctgtttga caatccacgg tccttcgaac atctgttcac
      481 cgactcaacg gagggtgggg agctagatag ttctcagctt gatccggtga ctgatcgaat
      541 cttggccgga tctcacgccg atcgatcact ctatgtcctt ttgacaacgc tcatacaaac
      601 gggtgatcca aaacttttgc gggccgctga aatgagttta cgcagcttag taaaacaatc
      661 tcgccaagcg accgtgcctt ggcaacaacg cccctccggt cacttcgaac caccgagcga
      721 ttagtcctct ctgatttctt cccttgatcg gtctctttcg agatctactg ccagctcgcc
      781 gccaacacag cctgcacatc ctgtggtcgc tggtcgatcg cttgatccca ggtgagaccg
      841 gactgccgac tgaatgaggc catagcctga atcaactgat cgacctgcgt gctgagtaac
      901 gtttgtccat tgccggcctg gatggtctcg attcgattcg ttgtatcaag ataccagttc
      961 tcgacagtca cttgatcaga tgaaccatga atgacgagtc tcagattgtt ggcttgacgg
     1021 ctgatcacca aatccaaggg attgattgtc gcatcaaaga tggcacgatc ttggttgctc
     1081 gagaaaggat cagaatcgat gatcgtgtcc tgtccatcac ctcgcaagag attatagaca
     1141 tcgttgcccg aaccgccggt catcgtatcg ttaccggcgc cactggcgaa ttgatcagcg
     1201 ccgctgccac ccgtaagggt atcgtttcct gcgccgccca ccagttgatc atcacctgaa
     1261 cccccgtcga gcgtgtcgat tccgtctcct ccatacaaaa catcgcttcc gctcccgcca
     1321 ttcagagcgt cgttgccgag tccgcccaat agttgatcat tgccgctgct ccctaagaga
     1381 tgatcatcac ccgccccgcc atcagccata tcattgccgg aaccgccgtc gagagtatta
     1441 ttgccgatgt tcccgacaag cacgttattg gccgaattcc cagtgccgtt gatggcagcc
     1501 gcacccgtca acgtcaggtt ttcaacgttg ccacccaaca tataggtcaa gttgctttcg
     1561 atggtatcaa tgccctgatt cgacgattca gtcacgacgt ccccggtctg gctgaccaca
     1621 taagtatcgt taccgatgcc accaaccatc gtgtcgttgc cgaactcacc gtcgagccga
     1681 tcgttcccag ctccgccagt caatgtatta ttagcgctat tgccaatcat gatgttattc
     1741 agtgtattcc ccgtgccgtt gatcgcgctt gtgccagtca gtgtcaggtg ttccacattg
     1801 ttgccgagtg tgtacgtcat tgaactttgc gctgtgtccg tcccttcatt cgctgcttct
     1861 atgaccagat cacttgatga atcaattatg tatgtgtcgt cacccacctc accacgcatt
     1921 gtgtcggttc cagttcctcc atccaggaca tcgtctccgc ttccaccatt cagcgtatcg
     1981 ttcccggcca atcctctgat ccgatccacc acatttgtgc cgttgaccga atcgttgccg
     2041 gttgtgccgg tcagatcaaa tcctcgcgca accagctggc tgtagttcaa cgtcgtgccg
     2101 tcggcaaaac ggaaggtctc gatcgaatgc gctcccaagg cattatttgg atcgaatggc
     2161 gtcaggtgta gtgcgtcacc attcgtcccg acacggatga tgaacgatcc gatcccgaga
     2221 ctgaggtcat caggcataat gcctgtccca aattggattg tattgccttc accaggaaga
     2281 ctcgtgtcgt gaaccgtgtc tactccatcg ccaagattga agaagtaggt gtcatctccc
     2341 gacccgccgg acaaggcatc gttccccgct ccaccagcga gggtgtcgtc tccccgccca
     2401 cccatcagtt catcatcgcc ggtagtaccg accacggttt gcggaagcgg tccagacacg
     2461 gtcagcgtga actgatcggt gatcccaacc tcctgtgtat cagtggcagt cacagctagc
     2521 cgcagtaccc cggcatcgcc tgggccaggg gtcccgttga atgtgcgcgt ggtcggattg
     2581 aaactcaacc agttcggcaa tgggctgcca tcagccagca ccgcattgta ggtgagaaga
     2641 tcgccatggg ccgcatcgtc gtcagcgaac gcgccactcg gaaccgtgaa gctgaacgca
     2701 gtatcttccg ccgcactctg atcagcg
//
LOCUS       DASTWE010000906         1438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1478576, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000906 DASTWE010000000
VERSION     DASTWE010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478576"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1426
                     /locus_tag="VFT30_09445"
     CDS             191..1426
                     /locus_tag="VFT30_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HEU5092899.1"
                     /translation="MAYRLITIDALRIGMHVAKLDVAWFRSPFLRHSFLISTHEQIEK
                     LRRAGVRQLTIDPARGLDLPETSTLSEAKPQMAQNGCPATVSPPTVRSLARMTEELEA
                     ARSARRQLELSVQSTFSRVAETGTVDPEKAQHIIHEINAVAKSLTTHALFMAFSHGRD
                     GSHSLSEHALATCSFSMILAHAAAYDLSALQDLATGALLHDIGLLQVPRSLLERIHDT
                     SITQSEATRRTYESHARSGAILLERQGGFSSAVEQIVAEHHAYLDQSGFPAETRGTFT
                     SDMTRLIMVTDRYDELLTGFGGASPLTPHQSLQRVYQEGLEGKYENRLVSLFVKVMGI
                     YPVYSSVMLTTGERAVVSLVNSSKLHLPVITITHDPSGTPYIVPLVIDLANQNEKAPM
                     RGICSVIGTIASDIEKAVR"
BASE COUNT          309 a          450 c          379 g          300 t
ORIGIN      
        1 gatcggaaca atccccggat ggacatacgg ttgtcggtcg tcccgctcat atcgcctgaa
       61 cgggccggcc ttgtcgagca cgccgcatcg gtcgccacac ctgccattca tacagccccc
      121 aagcagcgac agtaatagga gtggatgagg gactgcttag gcctccacga atcgcctgtt
      181 ctgatattct atggcatacc ggctgatcac cattgatgcg ctacgaatcg ggatgcacgt
      241 cgcgaaactc gatgtggcct ggtttcgatc gccttttttg cggcactcgt ttttaatcag
      301 cacgcacgag caaattgaaa agttacggcg cgcgggagtg agacagctca caatcgatcc
      361 ggcccgtggc ctcgatctgc cggagacttc cacactttct gaggccaagc cacagatggc
      421 tcagaatggc tgcccagcga cggtcagtcc gcccacggta cgttccctcg ccaggatgac
      481 agaggaacta gaagccgcgc gatcagcccg tcggcagttg gagctatcgg tgcaatcgac
      541 gttctcgcgc gttgccgaaa cagggactgt cgatccggag aaggcgcaac acatcattca
      601 cgagatcaat gcggtggcca agagtctgac cacccacgcc ctgttcatgg ccttcagtca
      661 tggccgtgac ggaagtcact cgctcagtga acatgcccta gccacctgca gtttttcgat
      721 gatcctcgcg catgccgccg cctatgactt gtccgccttg caggaccttg ccaccggagc
      781 actcttgcac gacatcggcc tgctgcaagt gcctcggtct ctgctggagc gtattcacga
      841 tacgtccatc acccaatcgg aggcaacccg gcgaacctat gaatctcacg cacgctccgg
      901 cgcaatcctg ctcgaacgcc aaggcggctt ctcttcagcc gtcgaacaaa tcgtggcgga
      961 acaccacgcc tacctggatc agagcggatt tccggctgag actagaggga cattcacctc
     1021 ggacatgacc cgtctcatca tggtgaccga ccgttatgat gaattgctga cgggattcgg
     1081 cggcgcatcg cccctgaccc cccaccaatc gttgcagcgg gtctaccagg aaggcctgga
     1141 gggaaagtac gagaaccgat tggtctcgct cttcgtcaag gtgatgggca tttacccggt
     1201 ctatagttct gtcatgctga ccactggtga acgcgcggtc gtttcgctcg tcaactcatc
     1261 gaagctgcac ctacccgtca tcaccatcac ccatgaccca tccggcacac cctatatcgt
     1321 tccactcgtc atcgacctcg ccaatcagaa tgaaaaggcg ccaatgcggg gtatttgttc
     1381 agtcattgga accatcgcgt cagacatcga aaaggcggtt cgctgatcgt cgaccttt
//
LOCUS       DASTWE010000907         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1187615, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000907 DASTWE010000000
VERSION     DASTWE010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187615"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1257)
                     /locus_tag="VFT30_09450"
     CDS             complement(94..1257)
                     /locus_tag="VFT30_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU5092900.1"
                     /translation="MTALNRGESYIPDVESQILCRLIEAGRYRATSNMDALAESDAII
                     ICVPTPLRKTKDPDISFVYSSAAEVKARLRKGQVIVLESTTYPGTTKELLLPMFEESG
                     LTVGKDFFLAFSPERIDPGNQQFQVRDIPKVVGGVTPECSRIAALLYGQVVTKVTEVS
                     SPTVAELAKLYENTFRSVNIALANEFAMMCRYLDVSVWEVIEAASTKPFGFMPFSPGP
                     GIGGHCIPIDPIYLSWKLRLSGYEARFIALADEVNRSMPRYVIELLMDALNTREKCLK
                     GSRILVLGVAYKRGVGDMRESPALDVINELQRKGADVRYADPFVPSLEHEGLTTPRVD
                     ATMETIRAADAVVVITDHKEFDYPMIVQTADLIIDARNATHGLSAPDSRLIRL"
BASE COUNT          315 a          355 c          300 g          291 t
ORIGIN      
        1 gcaaactccc tcgttgctca ccgggaaggg cggcctcgaa ctccatcccg tgataatatt
       61 cttctacatc gacacttaac gcgttggtaa tcatcacagt ctgatcaagc ggctgtcggg
      121 agcggagaga ccgtgagtgg catttcgtgc atcaataatt aaatctgcag tctgcacaat
      181 catgggatag tcaaattcct tatggtcagt gatgaccaca acggcatctg cggcacggat
      241 cgtctccata gtcgcgtcca cccgaggtgt ggttaagcct tcgtgttcaa gagacggaac
      301 aaacggatct gcataccgaa catcggcccc tttgcgctga agttcgttga tcacatcgag
      361 agccggagat tcacgcatat cgcccactcc tctcttgtaa gcgacgccaa gcaccaggat
      421 gcgcgaaccc ttcaggcatt tttcccgtgt attcagcgca tccatcagca gctcgatcac
      481 ataacgcggc atactgcggt tcacttcatc tgccagcgca ataaagcggg cctcatagcc
      541 actcagacgc aacttccagg acagataaat cggatcgatg ggaatacaat gtccgccaat
      601 ccccggtccc ggagaaaacg gcataaaacc aaacggcttt gtcgatgccg cctcaatgac
      661 ttcccacaca ctcacgtcga gatagcggca catcatggca aattcgtttg ccagcgcgat
      721 attgacgcta cggaaagtgt tttcatacag tttggcaagt tcggcgaccg tcggactcga
      781 cacttcggtc acttttgtca ccacctgccc atataaaaga gccgcaattc gagagcactc
      841 tggggtcaca ccaccaacga ctttcggaat gtcgcgtact tgaaactgct ggtttccggg
      901 gtcaatgcgt tcgggggaaa aagcgagaaa gaagtctttt cccactgtga gcccgctttc
      961 ttcaaacatg ggcaatagca attccttggt cgtcccggga tacgtcgtac tctccaacac
     1021 aatgacttgg cccttgcgta accgagcttt cacctcggcc gcagaggaat agacaaatga
     1081 aatgtctgga tcttttgttt ttcgcagcgg agtaggaaca caaataatga tagcatcgct
     1141 ttcagccagc gcgtccatat tactggtcgc tcggtaccgc ccggcctcga tcagacgaca
     1201 gaggatctga ctttccacat cgggaatata cgattctcca cgattcagag cggtcacttt
     1261 g
//
LOCUS       DASTWE010000908         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_670761, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000908 DASTWE010000000
VERSION     DASTWE010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670761"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VFT30_09455"
     CDS             <1..1280
                     /locus_tag="VFT30_09455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5092901.1"
                     /translation="QYERNDPQLLGQIPPTTRVIRAKGRDPWRAFQTRRGTQTKNRLS
                     GLSTEDVRQVVASHRAPWRSRLREWVRTAEAWVYRPDMAMPWIGPATKVTIDLCRRNR
                     PNVIWATIGPMSAGEVAYRTSVATGVPYVLDFRDPWGLEYYPHEIRRPTWAKNIDNRN
                     MRRIFERAQAVVFMFKSVAESYLQAFHGALDRTKIHIIPNGFEGEVESFVHTPGDRCT
                     ILYAGTLTTYRYDTLLEGLTQLKRQDPALAVRLRLLFVGEGFQQLAERVADLGLQNLF
                     EIIPPVPSAEVRRLQREAHALLVLGRMPGRTAHELVAGAKLFGYLQAGRPIIGIVPHD
                     ETRRILGQVGSSMIADADTPAEVVTVLERILEAWSNGTLERFVPNRAACEAYSSDRQI
                     SDLITALNGASPEKFVTGEMPTSRPSLRSEVVR"
     gene            1277..>2206
                     /locus_tag="VFT30_09460"
     CDS             1277..>2206
                     /locus_tag="VFT30_09460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5092902.1"
                     /translation="MKIVYVNDILYGYATGDSSAKGGAERYGWHLTRALAKVGWSVTV
                     GVRSALQEGKEQTVDGVRFLGIGSQTHFLVAWYRFLKKERPDWCFWQCADHLWGPTAE
                     IARRLGVRTAFSMMHDNHVQLRKALTRRGNLWPLYAWGLQRSDVIFIQHDGQRVHLPM
                     RWQGRAYPLPGIVPLTSTVVPHSERNGAVLWIAVIRPAKRPDLLIEIASRLPTIRFIV
                     CGAPSLGHWETGKIEPIMKQLQSLPNVDYRGHVSPEQALNIIGEASLLLSTSDGEGFP
                     SVFLEAWAAGTPVVSIQIDPDHKIRNCDLGKVTE"
BASE COUNT          516 a          550 c          643 g          497 t
ORIGIN      
        1 atcagtacga acgaaacgat ccgcagctat tgggtcagat accgccgact actcgggtaa
       61 ttcgagccaa aggtcgcgat ccctggcgtg cgtttcagac cagacgcgga acgcaaacga
      121 agaacaggct ttccggctta tccacggagg acgtgcgaca ggtcgtcgcc tcacatcgtg
      181 caccgtggcg ctcaaggctg cgtgaatggg tgagaacggc agaggcatgg gtgtaccgtc
      241 ctgatatggc gatgccatgg ataggacctg ccactaaggt aaccatcgac ttgtgccggc
      301 gaaatcgtcc gaatgtgata tgggcgacga ttggaccgat gtcggccggc gaggtggcct
      361 atcgcacatc agtggcgaca ggggttccgt acgtgttgga ttttcgggat ccttggggat
      421 tggaatatta cccgcatgaa atcaggcgtc cgacttgggc caagaacatc gacaatcgga
      481 acatgcgtcg aatctttgag agggcacagg ctgtcgtctt catgttcaag tcggtcgcag
      541 aatcctacct acaggcattc cacggggcat tggatagaac gaagattcat atcatcccaa
      601 acgggttcga aggggaagtt gagtcttttg ttcacactcc cggggatcgt tgtacgattc
      661 tgtatgcagg cacgcttacc acgtaccgat atgacaccct gcttgagggg cttactcaac
      721 tgaaacgaca agacccagcg ctggctgtac ggttgcgact gttgttcgtc ggcgaaggat
      781 ttcagcaact agcggaacga gtggcagact tgggccttca gaatctattc gagattatac
      841 ctccggtccc aagtgcggaa gttcgtcgcc tccaacgaga agcccatgct cttctggtac
      901 tgggcagaat gccgggaaga acggcgcacg aactggtcgc aggagccaag ctattcggtt
      961 atctacaagc gggacgaccg atcatcggaa tcgtgcctca tgacgaaacg cgacgaattc
     1021 ttgggcaagt cggaagctca atgatcgcgg atgccgatac tccggctgaa gtggttacgg
     1081 tacttgagag gatcctcgaa gcttggtcga acgggactct cgaacggttt gtcccaaatc
     1141 gtgctgcctg tgaagcctac tcgtccgatc ggcaaatttc agaccttatt acagctttaa
     1201 atggcgcgtc tcctgagaag tttgtgacag gggagatgcc gacctcgcgg ccttctttgc
     1261 ggtcggaggt tgtacgatga aaatcgttta tgtgaacgac attttatatg gatatgccac
     1321 cggagattct tcggcaaagg gcggggcaga gcgctatgga tggcacctga cacgtgcctt
     1381 agcgaaagtc ggctggtcgg ttacggtagg agttcgctct gcattgcagg aaggaaaaga
     1441 acaaacagtc gatggtgtgc gcttcctcgg gatcggcagc caaacacact tcctggtggc
     1501 ctggtacaga ttcctaaaaa aagaacggcc ggactggtgc ttctggcaat gtgctgatca
     1561 tctgtgggga ccaactgcgg agatagcacg aaggcttggg gttcgaacgg ctttttccat
     1621 gatgcacgat aatcacgttc aactcaggaa agcgctcaca cggcgaggga atttgtggcc
     1681 tctctatgca tgggggcttc agcgttcaga cgtcattttt attcaacatg atggtcagcg
     1741 tgttcatctt cccatgcgtt ggcagggaag ggcctatcct ctcccaggca ttgtgccgct
     1801 gacgagtaca gttgtgcctc attcagaacg aaatggagca gtcctctgga tagcagtcat
     1861 caggccggcc aaacgaccgg atcttttgat tgaaatcgcc agccggctac cgacgattcg
     1921 ctttatcgtc tgcggagctc ccagtttagg tcattgggag accggaaaaa ttgaaccgat
     1981 tatgaagcag ttacagtcgc ttcccaacgt ggactaccga ggccacgtct cccctgagca
     2041 ggctctcaac atcatcggcg aggctagcct tcttctctcg acgtcggacg gagaaggatt
     2101 tcccagtgtc ttcttggaag cctgggcggc ggggacaccg gtagtgagca ttcagattga
     2161 ccccgatcac aaaatcagga attgtgactt gggaaaggtg acggaa
//
LOCUS       DASTWE010000909         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_218084, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000909 DASTWE010000000
VERSION     DASTWE010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218084"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFT30_09465"
     CDS             <1..387
                     /locus_tag="VFT30_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092903.1"
                     /translation="GAHPGIKKWHDRVANQIKRFRFVENRFGYRWYIFDRIDSLLPEA
                     IAWIPQSTVSIVINRIWMNIYDRLPSVEVLLQVHDSLCGQFHTSQKDIIIPQIKDAGR
                     IVIPYEDPLVIPFGLKTSEVSWGEVK"
     gene            552..>1047
                     /locus_tag="VFT30_09470"
     CDS             552..>1047
                     /locus_tag="VFT30_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092904.1"
                     /translation="MTDSADSPSTYKRNFDDWLTSYCQYASFSEAPTTMHFWSGVSAI
                     AGALRRRVWLDMGYFKWTPCFYIIIVAPPGVVSKSTTAAIAIDLLRQVPGIKFGPQMV
                     TWPALVKAFADSKEEFELGDEWHPMCAMTIESSELGNLINPQDREMIDLLVTLWDSKT
                     GALDK"
BASE COUNT          260 a          275 c          247 g          265 t
ORIGIN      
        1 ggtgctcatc ctggaatcaa aaaatggcac gatcgagttg ctaaccagat taaaagattc
       61 cggttcgtcg agaatcggtt tggttaccgc tggtatatat tcgatcgcat cgatagcctg
      121 ttgccggagg caattgcctg gatccctcag tccaccgtca gcatcgtcat caatcgaatc
      181 tggatgaaca tctatgatcg tctaccgtcc gttgaggtcc ttttacaggt ccatgactca
      241 ctatgcggac agttccacac cagccagaaa gatattatca ttccgcaaat taaggacgcc
      301 ggccgtatcg ttattcctta tgaggatccg cttgttatcc cgtttgggct caagacctcg
      361 gaagtttcct ggggtgaggt gaagtgagat gaaaaagatc gaacagccga ctttgaacga
      421 actcgccggc gccatagcca tcttagcagt cgtgtttata gttggctttt ggattggtta
      481 tatgtttggc agcgaaacct aacaataacc gccgtcaaca attcacccga ttatcaacgt
      541 cgagatcgta aatgactgac tcagccgact ctccatcaac ctacaagcgc aacttcgatg
      601 actggctcac ctcatattgc caatacgctt ccttcagcga agcccccacc accatgcact
      661 tttggagcgg agtatccgct attgcaggtg ccttacgtcg aagagtatgg cttgacatgg
      721 gatattttaa gtggacgccg tgcttttata tcatcatagt cgctcctcca ggagttgttt
      781 ccaagtccac tactgcggct attgctatcg atttacttcg tcaagtaccg ggtattaagt
      841 tcggccctca aatggtcacg tggccggcgc tcgtaaaggc ctttgccgat tccaaagagg
      901 aattcgaact cggtgacgaa tggcacccga tgtgcgcgat gacgattgaa tccagcgaac
      961 tagggaacct catcaaccca caagaccggg agatgatcga cctcctagtt acgctgtggg
     1021 actccaaaac cggcgccctc gacaagc
//
LOCUS       DASTWE010000910         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1446498, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000910 DASTWE010000000
VERSION     DASTWE010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446498"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..822)
                     /gene="tatA"
                     /locus_tag="VFT30_09475"
     CDS             complement(22..822)
                     /gene="tatA"
                     /locus_tag="VFT30_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5092905.1"
                     /translation="MFGTMGFSELIIILVIVLIIFGAGRLPQIGEGVGKALKGFRKEV
                     NEVPQPASGQVQQETGPSQTIQTPTNIAATVPPPTTGSPATTRPNAPYVPGPELTPGT
                     TASLLYNTSLQTPQTSAPSSNQASASASNTVLSMEERAAAPPPRAHASYPPLPAGSQA
                     KATAKRPSAIVNKDAVARVQAQQAALKAKPLQSAATVSPDDLQHLGEGLGEVVRTFRQ
                     AVTDVRNSVDPQMSTIQAEMDAAKKEIQQSIEAAKELPPMQEDSQKSA"
     gene            complement(906..2108)
                     /locus_tag="VFT30_09480"
     CDS             complement(906..2108)
                     /locus_tag="VFT30_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5092906.1"
                     /translation="MTGVAHQTRIVAIQEALGDMGTVDGWLFYDFRGSDPLAYRVLLL
                     DPTQHVTRRWYYWIPTCGEPVKLLHKIEPHMLNELPGQVQQYVSWEQQRMLLTSLLRG
                     RRRIAMQYSPFNAVPYLSRVDAGTVELIRSFGVEVVTSANLVQRFEAVWTDEQLESHR
                     YAVTTLRNIVDEAFSHVASCLTNERPLSEYGLQQFILSRIRDAGMTTSSAPIAAVDAH
                     SADPHYGPTESGSSLIMRDNLVLIDLWAKQTAPGSVYGDITWTGYTGIQVPAKQRMIF
                     GHVRRGRDAALSFVQNQVAIGRFPCGWEVDDVCRNTIRAAGYDDFFIHRTGHSIGEEV
                     HGNGANIDNLETQDDRRLLPGTCFSIEPGIYLPGEFGIRSELDVYVSDREAVVYGLPL
                     QTEIIPLL"
     gene            2188..2658
                     /locus_tag="VFT30_09485"
     CDS             2188..2658
                     /locus_tag="VFT30_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092907.1"
                     /translation="MVLESTRELPSYRVTFFFGPESVKGQPDVLACVFNVKKRSWKAG
                     IQVSVEMGTDQLSFLREKMRLADRLEKSLLALDPRECPHYQERAAEFFAQAVCWCKLD
                     LKLQAGLTQENQRIRADELTIELDLEAGNRVDYVLAYILGELDLTPDRSVPSSR"
     gene            2779..3159
                     /locus_tag="VFT30_09490"
     CDS             2779..3159
                     /locus_tag="VFT30_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092908.1"
                     /translation="MRLPIVVLIVVITYCSILVAPAWSQSSSAETQQGTYSGAGMGAA
                     SAVATILYFPFKAAFAIGGGIVGGLAYVFSGFSEPTAKSIWVPSILGDYAIIPEHLSG
                     DRPIRFLGVAAENGGTAVTPAPTN"
     gene            3175..>3729
                     /locus_tag="VFT30_09495"
     CDS             3175..>3729
                     /locus_tag="VFT30_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU5092909.1"
                     /translation="MKPVIGVTPDFNAGDRKDMGGHEPTYFLRARYIRAVEELGGIPL
                     ILPLVAEPSARRRLLDRVDGLLIPGSGPDLPPRLYGERQRYKFPLVSERRADFELELV
                     LQARKRDLPLLGICGGMQTVNVACGGSLYQDIPAQVPGALDHRQKAKAVHVAHKVIVT
                     PKSLLNKVVARRTLMVNSSHHQSVK"
BASE COUNT          818 a          956 c         1050 g          905 t
ORIGIN      
        1 ggggatccgt tgagatgaga atcacgcaga tttctgagag tcttcttgca tcggtggtag
       61 ttctttagcc gcttcgatgg attgctggat ttcttttttg gccgcatcca tttcggcctg
      121 aatcgttgac atttgaggat cgacgctatt gcggacatcg gtcacggctt gtcggaacgt
      181 tcgcacgact tctcccaggc cttcaccgag atgctgcagg tcatcgggcg ataccgtcgc
      241 tgctgattgc agcggcttgg ctttgagcgc ggcctgttgt gcttgtacgc gagcaaccgc
      301 atctttgttc acaatagcgg aaggacgttt agcggtcgct ttagcttggg aaccagccgg
      361 caaaggtgga tacgaagcat gagccctcgg agggggagcg gcagcccgtt cctccatcga
      421 tagcaccgtg ttggacgcag atgctgatgc ttgattggag gaaggagccg aggtctgcgg
      481 agtctgaaga cttgtgttat acagcaacga ggcagtggta ccgggtgtca gttcaggacc
      541 agggacgtaa ggagcgtttg gtctagtcgt tgctggtgat cccgtggtcg gaggtggaac
      601 tgtcgctgcg atgtttgtcg gagtctgaat tgtctgcgat ggcccagttt cttgttggac
      661 ctgtcccgaa gcgggttggg gcacttcgtt gacttctttt ctaaaccctt tcaatgcctt
      721 gccgacgccc tcgccgatct gcggcagacg gcccgctccg aaaatgatca gcacgatgac
      781 cagaatgata atgagttccg agaaacccat tgtgccgaac atgtacggcc tcgcatgtga
      841 ttggggaagc gcctatgccg gatgagtgaa tctagctgcc tcttagatac ggtgtcaaga
      901 aagggtcaga gcagaggtat gatttctgtt tgaagtggta agccatacac gactgcttct
      961 cgatcggaca catagacatc aagttcactc ctgatcccaa actctcccgg cagatagatc
     1021 cccggctcaa tagaaaagca agttcccggc aggagacgac gatcgtcctg agtctccagg
     1081 ttgtcgatat tggctccatt accatggacc tcttcaccga tcgaatgacc ggttcgatgt
     1141 atgaaaaagt cgtcgtagcc agccgctcga atcgtattgc ggcagacatc atcgacttcc
     1201 catccgcaag gaaatctgcc tatagccact tgattctgaa caaacgacag tgccgcatcg
     1261 cgtccgcgcc gaacatgtcc aaagatcatg cgctgctttg ctggcacctg gatgccggta
     1321 tagcctgtcc aagtgatatc cccataaacc gaacccggtg ccgtttgttt tgcccataaa
     1381 tcgattagaa ccaaattgtc tcgcatgatc agcgaagagc ctgactcagt cgggccgtaa
     1441 tgtggatcgg cactatgggc atcaacggct gcaatcggag cactggatgt cgtcatcccc
     1501 gcatcgcgaa tacgtgagag aataaactgt tgtagaccat attcagataa cggacgttcg
     1561 ttggtgagac aagacgccac atgggagaag gcttcgtcga cgatgttcct gagcgtcgtc
     1621 acggcgtacc gatgagattc cagttgctca tccgtccaga ctgcctcgaa tcgttggacg
     1681 aggttggctg atgtaacgac ttcgactcca aaacttcgaa tcaactcgac ggtacctgca
     1741 tcaactctgg acagatacgg aacagcgttg aacggtgagt actgcatggc gattcgccgt
     1801 cgacctcgca acagggaggt caataacata cgttgctgtt cccatgagac gtactgctgg
     1861 acctgaccgg gtaactcatt cagcatatga ggttcgattt tgtgaaggag tttgactggt
     1921 tctccacatg tagggatcca atagtaccag cgccgagtga catgctgggt cggatcaagg
     1981 agcaaaaccc gataggcgag ggggtcactg ccgcgaaagt cgtagaaaag ccatccatct
     2041 accgtgccca tgtctccgag tgcttcttgg atcgcaacga tccgcgtttg gtgggccaca
     2101 ccggtcatgg gttcccatct tatcttcgga gtatagaggc gtcaatcagc ccgcaggtct
     2161 ccgcgtgaac atggtacaat ggctcccatg gttctagaat caactcgaga gctcccgtct
     2221 tatcgagtca ccttcttctt cgggcctgag tcggtaaaag gacaacccga cgtgctggcc
     2281 tgtgtgttca atgtgaaaaa acggagttgg aaagcaggca tccaggtttc tgtcgagatg
     2341 ggcaccgacc aattgtcttt tcttcgagag aagatgcgtc ttgctgatcg gctcgagaaa
     2401 agtttgttgg cactcgaccc ccgcgagtgt cctcactatc aagaacgcgc agcggagttc
     2461 tttgcacaag ctgtttgttg gtgcaagctg gatctcaaac tgcaggctgg tctgacccaa
     2521 gaaaatcagc ggatccgagc cgatgagttg acgatcgaat tggatctgga ggcaggcaat
     2581 cgcgtcgact acgttctcgc ctacattttg ggagagttgg atctcacccc tgatcgttcg
     2641 gtgccatcat cccgttagta tcgaggtcaa gagggggttg cattcagcag cgaatttgtt
     2701 ataacgcatg ccaggactag tgtatgatcg ttctgcattc ccatcgttgt ggatttgaaa
     2761 aggaaggagg cgtttcacgt gagactccca atcgtggttc tcatcgttgt catcacctac
     2821 tgttcgatac ttgtcgctcc tgcctggagt caatcgtcat ccgctgagac gcagcagggg
     2881 acctattccg gagccgggat gggagctgcc tcagccgtcg ccacgatctt atatttcccg
     2941 tttaaagcgg catttgccat agggggggga atagtcggcg gtctcgccta tgttttttca
     3001 ggattcagcg aaccaacggc caagagtatc tgggtcccca gcattcttgg agactatgcc
     3061 attattcctg agcatcttag cggtgatcgt ccaatccgtt ttctcggtgt tgctgccgaa
     3121 aatgggggaa ctgccgtcac gcctgccccg acgaactagc ctgtgcattg aattatgaaa
     3181 cctgtcatcg gcgtgacccc tgactttaat gctggtgaca ggaaggatat gggcggacac
     3241 gagccaacct acttcttgcg ggctcgctac attcgcgccg ttgaagagct cggcggtatt
     3301 cctttgatcc ttcctctggt agcggaaccg tctgcccgac gacggcttct tgaccgtgtc
     3361 gatgggctgc tcatccccgg cagcggaccg gaccttccac cgcgtctcta tggagagcgg
     3421 cagcgctaca aatttccgct cgtgagtgag cgccgcgccg actttgaatt ggagttagtc
     3481 cttcaagcaa ggaaacggga tcttccgctc ctgggaatct gtggaggaat gcagacggtg
     3541 aatgttgcct gtggggggag tctgtatcag gacattcccg cgcaagtccc cggcgcgttg
     3601 gatcatcgtc aaaaagcgaa ggctgttcat gtggctcata aggtgatcgt gacgccgaag
     3661 agcctcttga acaaggtggt cgcaaggaga acgctcatgg tgaatagttc ccaccatcag
     3721 tcggtcaag
//
LOCUS       DASTWE010000911         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_89019, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000911 DASTWE010000000
VERSION     DASTWE010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89019"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1937
                     /locus_tag="VFT30_09500"
     CDS             <1..>1937
                     /locus_tag="VFT30_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5092910.1"
                     /translation="MAAGIIWHFSQDLPSLDLLQNYQPSLVTTVYSDDRQPIGQFFIE
                     RRILTPLPEIPKTLTQAIIATEDARFFEHPGLDYIGMLRAAWTNIRHGGRKVEGASTI
                     TQQLARSLFLSSERSYERKIRELILAYKMEAVSGKEQILETYLNQIYFGQGAYGVGSA
                     AHSYFGKDIRSLTLAESAFLGGLPKSPSRFSPFTAYELAKNRQEHVLARMEEVGFITA
                     AERESAAREKLNFHRPGSEHLAPYFVEYVRQMLVAKYGESMVYKGGLQIYTTLNLEMQ
                     KAAESAFLNGVRELDKRQGWRGPRRAVDLATYQPSGLAQGDQLLKPGYFGEGVVLKVA
                     KDHYVVQVGAFTAKLAFDDMVWAKRILKGPDPAVDFIVNPNLKSLLKPGDVIEIGVKK
                     LTKDGVQLTLEQTPIVEGGLIAIDPKGGAIRAMVGGYDFSRSEYNRAVQAHRQPGSAF
                     KPLIYATAMSQGLSPATQILDAPVVYEQEEDDKIWKPENYGRKFHGMVSLRDALAQSH
                     NLATVRLLDKVGVKNVIEFSRTVGVTSPLPSDLSLGLGTSSVGLLELTSVYGVFLNQG
                     SRVEPFAVKSVKDNMGKTLEVTEPESHEVIAKETAYLITNMMEDVVQRGTGQSAKVLG
                     RPIAGKTGTTNDYINAWFIGG"
BASE COUNT          458 a          500 c          551 g          428 t
ORIGIN      
        1 gatggccgca ggaatcatct ggcacttttc ccaagatctt ccatcgctcg acctgctcca
       61 aaactaccaa cctagtttgg tcaccactgt ctattcagat gatcgccaac ccatcggcca
      121 attcttcatc gaacgccgta tcttgactcc gctccctgaa attccaaaaa ccctgacgca
      181 ggccatcatt gcgacggaag acgcacgatt tttcgagcat ccggggttgg actatattgg
      241 gatgttgcgg gcggcttgga cgaacattcg gcacggagga aggaaggtcg aaggagccag
      301 cacgatcaca caacaactgg ctcgttcgct cttcctctcg tccgagcggt cctatgaacg
      361 gaagattcgc gagcttattc tcgcctacaa aatggaagcg gtctctggta aggagcaaat
      421 ccttgaaacc tacttgaacc agatctactt tgggcagggg gcctacggcg tcggctcggc
      481 ggcccattcc tatttcggga aagacatcag aagcctgacc ctggccgaat ccgcgtttct
      541 gggtggtctg ccgaagtcgc ccagccggtt ttctcctttc accgcctatg aattggcaaa
      601 aaatcgccaa gagcatgtgc tcgctcgaat ggaggaggtt ggatttatta cagctgcgga
      661 acgggaatcg gccgctcgtg aaaaactgaa ttttcaccgg cctggaagcg aacatcttgc
      721 gccgtacttc gtcgaatacg tccgccaaat gctcgtcgcg aagtacgggg agtcgatggt
      781 gtacaagggc ggtctccaaa tttacacgac cttgaattta gagatgcaaa aagcggcaga
      841 gtcggcgttc ttgaacggcg ttcgcgagct cgacaaacgg caagggtggc gagggcctcg
      901 gcgagctgtc gatttggcga cctaccagcc gtctggcctg gctcaggggg atcagctact
      961 caaacccgga tatttcggtg aaggagtcgt cttaaaggtt gcgaaagatc actatgtggt
     1021 tcaggtcggc gcattcacgg cgaaactggc ctttgacgac atggtgtggg ccaagcggat
     1081 tctcaaaggg ccagatcccg cagtggattt catcgtgaat ccgaacctca agtcactcct
     1141 gaagcctggg gatgtcattg aaatcggcgt gaaaaaactc accaaagatg gagtgcagct
     1201 tacattggag cagacgccga tcgtcgaggg aggcttgatt gcgatcgatc ccaaaggagg
     1261 tgcgattcgt gcgatggtgg gtggctatga cttctcccgc agtgaataca accgtgcggt
     1321 gcaggcccac cggcaaccgg gatccgcgtt caaacccctc atttatgcga ccgcgatgag
     1381 tcaagggttg agccctgcca cgcagatcct cgacgccccg gtcgtctatg agcaagaaga
     1441 agacgacaag atttggaagc cggaaaacta tggtcgaaag tttcacggta tggtgagcct
     1501 ccgggatgcg ctggctcagt cacacaatct tgcgacggtt cggttgttgg acaaggtcgg
     1561 cgtgaaaaac gtgatcgagt tttcgcgtac cgtcggagtc acgagtccgc tcccttccga
     1621 tctgtccctg ggactgggta catcttcggt cggcttgctg gagttgactt cagtttatgg
     1681 cgtattttta aatcagggga gtcgagtgga accctttgcc gtcaaatcgg tcaaagataa
     1741 catgggtaag acgcttgaag tgaccgagcc tgagtctcat gaagtcatcg ctaaagaaac
     1801 cgcctatttg attaccaaca tgatggaaga cgttgtccag agaggaaccg ggcagtcggc
     1861 gaaggttcta ggacgtccga tcgccgggaa aaccggcacg acgaacgact acatcaatgc
     1921 ctggtttatc ggtggta
//
LOCUS       DASTWE010000912         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1511060, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000912 DASTWE010000000
VERSION     DASTWE010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511060"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFT30_09505"
     CDS             complement(<1..241)
                     /locus_tag="VFT30_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092911.1"
                     /translation="MNRVCFYGGLRDAYGEGFDLDVMTPAEMFRALYVQLPDFLKNIC
                     LGDYLVCREMPDGELALDEHLMEIRMSNTTFHLVPI"
     gene            complement(234..>1051)
                     /locus_tag="VFT30_09510"
     CDS             complement(234..>1051)
                     /locus_tag="VFT30_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEU5092912.1"
                     /translation="DRFGNELEPFLTLEVRNALTQHMVDEGDRECCGLITLSKGKYAY
                     VRSENVHPEPEKSFRFDKQTNVDLSRKKSIVAYCHSHPNGPQVPSQHDTEKQDQIKKP
                     AVIVARNPLTGVIDLFSFGDHLLEAPLDRRDFRYNVFDCLAALRSEVWQREGRYMPPA
                     FSENGWWLVGYRNPAEVRDNLNLYERNFERYGYREFTVDLENSSSPYAPKIGDVLMMQ
                     LGAPVINHVAVYVGNNLIYHHRQDKLSGHTPIGYVLGAGYVRKWVRHEGMWDE"
BASE COUNT          236 a          309 c          268 g          238 t
ORIGIN      
        1 taatcggcac aagatggaac gtcgtattcg acatacggat ttccatcagg tgttcgtcga
       61 gggcaagctc accgtccggc atttcgcggc aaactagata gtccccaagg cagatatttt
      121 tcaggaagtc aggaagctgg acgtaaagcg ctctgaacat ttcggcaggg gtcatcacgt
      181 cgaggtcgaa accttcgccg taagcgtccc gcagacctcc atagaagcag accctattca
      241 tcccacattc cttcgtgacg gacccatttg cgaacgtatc ctgctcccag cacatatccg
      301 atgggagtat gtccgctcaa cttatcctga cggtgatggt agatcaggtt gttgcctacg
      361 taaaccgcga catggttgat gaccggcgct cccaactgca tcatcaatac gtcgccgatc
      421 ttgggcgcgt aagggctgga cgaattctcc agatcgaccg tgaactcgcg gtagccgtac
      481 ctctcgaagt tcctctcata caggttcaga ttgtctcgga cctccgcagg gttgcgataa
      541 cccacaagcc accagccgtt ctcgctgaac gcgggaggca tgtacctccc ctcgcgctgc
      601 cagacttcgc tacgcaacgc cgccagacag tcgaaaacgt tgtagcggaa atcccgcctg
      661 tcgagcggag cctccagcaa atgatcgccg aagctgaaca agtcgataac gcccgtcagc
      721 ggattgcgcg ccacgatgac ggcgggcttc tttatctggt cctgtttttc cgtgtcatgc
      781 tgcgacggaa cctgaggccc attcgggtga gaatggcaat aggccacaat cgactttttg
      841 cggctaaggt caacattcgt ttgtttgtcg aaccggaagc tcttttctgg ctccgggtgg
      901 acattctccg accgaacata cgcatacttg ccctttgata acgttatcaa tccgcagcac
      961 tcgcggtcgc cctcgtcaac catatgttgc gtcagggcgt tgcgaacctc cagagtgagg
     1021 aacggttcaa gctcgttacc aaacctatcc a
//
LOCUS       DASTWE010000913         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1252471, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000913 DASTWE010000000
VERSION     DASTWE010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252471"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFT30_09515"
     CDS             complement(<1..642)
                     /locus_tag="VFT30_09515"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEU5092913.1"
                     /translation="MTADRLSDWEYSNLVCCVVLVTAAILLHWRTQAAAAPVGTDISH
                     YTVMRTLGPIKVDGVLDEPSWLAAKSTGAFRANDGSETVTSRVEAKMLWDDSNLYFAF
                     ECDDTDLVATMTKRDEHLWQEEVVEVFIDPDGDEKNYIELEVNPLGAFLDLFVLTPVV
                     PIPYESYNIPAKWAVKVDGTVQNSSDRDRGWTVELAVPLNEAVTAPNRPPKDGD"
     gene            complement(639..1214)
                     /locus_tag="VFT30_09520"
     CDS             complement(639..1214)
                     /locus_tag="VFT30_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092914.1"
                     /translation="MRHDLLARWQRLAFAAALVMTLTVNLDRVRVLANQAKGQAPQAP
                     PQEEKKDAEVPKAPKDDFMVQVYQALDAKKTEKLTHKERYKQDKSEEDVEVVLSYFNW
                     AVTDDDYWNRQISIDTKTTDGFLVEKIVKSGFGELGYVKQGDTKTQDVYRKEKVTVTG
                     GQATATMADAEIGPAEKERFHKLLDRMVLKK"
BASE COUNT          282 a          406 c          327 g          362 t
ORIGIN      
        1 atcaccatcc tttggcggcc ggtttggagc ggttactgct tcgttcaaag ggactgcaag
       61 ttcgacagtc cagccccggt cccggtccga ggaattctgt accgttccat ccaccttcac
      121 ggcccacttg gccgggatgt tgtaactttc gtacggaata ggaacgactg gagtcaacac
      181 gaacagatca aggaacgctc cgagcgggtt tacctcgagt tcgatgtagt tcttctcgtc
      241 cccatcgggg tcgatgaaaa cctctaccac ttcctcttgc caaaggtgtt catcgcgctt
      301 cgtcatcgtc gcaacaaggt cagtgtcgtc gcattcaaag gcaaagtaca ggttcgaatc
      361 atcccagagc attttcgctt cgaccctgct cgttacagtc tcggaaccgt cgttggccct
      421 aaatgctccc gtactcttag ccgctagcca tgaaggctca tcgagcactc cgtccacttt
      481 gatcggcccc agcgtccgca ttaccgtgta atgactgata tccgtgccca ccggcgccgc
      541 tgccgcctga gttcgccaat gaagcaggat tgctgccgtg accagaacca cgcagcaaac
      601 aaggttggag tactcccagt cgctcaatcg atctgctgtc atttcttcag taccatccga
      661 tccagcaact tgtggaagcg ttccttttca gccggaccga tttccgcgtc ggccattgta
      721 gcagtggctt ggccgccggt aactgtgacc ttttccttgc gatacacatc ctgagtcttt
      781 gtgtcgcctt gcttgacata accgagctct ccgaaaccgc tcttgacgat cttctcgaca
      841 aggaagccat cggtggtctt ggtgtcaata ctgatctgtc gattccaata gtcgtcatcg
      901 gtaaccgccc aattaaagta cgagagtacc acttcgacat cctcttcact cttgtcctgc
      961 ttgtaccttt ctttgtgcgt cagcttttcg gtcttcttcg catcgagtgc ttgatagacc
     1021 tgcaccataa aatcgtcctt aggagcttta ggaacttctg cgtccttctt ttcctcttgt
     1081 ggcggtgcct gcggtgcttg ccccttggct tggttggcaa gaactcggac cctgtccaag
     1141 ttcacagtca gcgtcattac caaggcagca gcaaaggcga gcctctgcca gcgagccaat
     1201 agatcgtggc gcatacagat acctccttaa ccgtgaaaca gttccctttt ttctgggcac
     1261 tggttgacac gccctaatgt cgggcatgat gaacgtgatt gattgcatta tagtcctgcc
     1321 gagaggccct gcaacacatt tcattgtcca gacatcgaat tcaaggatcc tgcggcg
//
LOCUS       DASTWE010000914         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1284617, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000914 DASTWE010000000
VERSION     DASTWE010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284617"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1626)
                     /gene="lysS"
                     /locus_tag="VFT30_09525"
     CDS             complement(142..1626)
                     /gene="lysS"
                     /locus_tag="VFT30_09525"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU5092915.1"
                     /translation="MDELNEQRQQRIKKLELLREAGVAPYGSRFEVKDRAGHLLALHG
                     EKTKETLEQEKVSCTIAGRVVALRRFGKAAFAVLQDGSDRLQVYLKKDLLSEQAYTIV
                     EQLDLGDWIGVTGTLFRTKTNEFTVEVHHLAFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPQVHQIFSIRSRIITGIRTFLIERGFLEVETPMMHPIPGGATAKPFVTHHNA
                     LGVDLYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVSYADF
                     QDLIVLTEELVSSLAQQILDKTVIPYQGKEIVLTPPWRRWSYHQAILEVNSLDPSVLY
                     DREKALAAAKRLNVPVDPKASLFTIVNEIFEETVEPSLQQPTFITDYPIEISPLARRK
                     DTDPNLTDRFELYIAGREIANAFSELNDPLDQRERFEGQAAQREAGDEEAHLVDEDFL
                     RALEFGMPPTAGEGIGVDRLIMLFTDQASIRDVVLFPQLRPEKS"
     gene            complement(1648..2794)
                     /gene="prfB"
                     /locus_tag="VFT30_09530"
     CDS             complement(join(1648..2724,2726..2794))
                     /gene="prfB"
                     /locus_tag="VFT30_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU5092916.1"
                     /translation="MLEDVEVRVRALAEQVSELRGHLDFAHMTADLQELEAQMGQPHF
                     WNDARAAAVVSRKKATIERELQQWRDIEAKMGDLDALLELAHESGDSTLETELASELN
                     HLEPRLATLRVELLLSGELDPNNAIVAIHPGAGGTESQDWAQMLLRMYVRWAEHKKFK
                     VETLDLLPGDEAGIKSVTISIMGPYAYGYLKAEAGVHRLVRISPFDSNKRRHTSFASV
                     FVYPELSEDIDVVVEEKDLRIDTFRSGGAGGQNVNKVETAIRITHLPTGIVVQCQNER
                     SQLQNRNGAMKILKARLFEVEQKKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQLVKD
                     HRTGHQISNVSSVMDGELDGFIEAFLKRKLGKGSDQLSPVGGPEDDL"
BASE COUNT          606 a          829 c          797 g          684 t
ORIGIN      
        1 tgatgccgac cgtaccgatt aacgccgcta cacccagggt aatcccggca accgatacga
       61 aggtgttcag cgagatcgtc cggttgcggc gcttggcgcg caggtagcgg agtccgacga
      121 agatttcata agggacagcc actacgattt ctctggtctg agctgtggaa aaagcacgac
      181 gtcgcggatg gaggcctgat cggtaaacag catgatcaat cggtcgaccc cgatcccttc
      241 tccggcagtc ggcggcatcc cgaattcaag ggcgcgaagg aaatcttcgt cgacgagatg
      301 ggcttcttca tcacccgcct cccgctgagc cgcctggcct tcaaaccgtt cacgttggtc
      361 caacggatcg ttcaattcgg aaaaggcatt cgcaatttcc cgcccggcga tataaagctc
      421 gaaccgatcg gtcaggttcg ggtccgtatc ctttcgcctt gcgagcggag aaatctcgat
      481 cgggtaatcg gtaataaagg taggctgttg cagactaggc tctacggtct cctcgaaaat
      541 ctcgttgacg atcgtgaaca acgatgcctt cggatccacc ggcacattca gccgtttggc
      601 agccgccaat gccttctctc gatcgtagag taccgatggg tcgaggctat tcacctctag
      661 gatggcttgg tggtaagacc atcgccgcca tggaggggtg agaacgattt ccttaccttg
      721 gtacggaatg accgtcttgt caagaatctg ttgagccagg ctcgagacaa gttcttcagt
      781 gagtacgatc agatcttgga aatccgcata ggacacgtag aactccagca tcgtgaattc
      841 cgggttgtgg atcgtcgaga tgccctcatt ccggaagttg cggttgatct cgaacacgcg
      901 cggaaatccg ccgacgatca ggcgtttcaa atacaactcc ggcgcaatgc gcaagtaaag
      961 atccacgccg agagcgttat ggtgcgtgac gaagggcttg gcggtagcgc ctccgggaat
     1021 cggatgcatc atcggggttt cgacttcgag gaaccctcgc tcaatgagaa acgtccggat
     1081 gccggtaatg atacggctcc tgatcgaaaa gatctgatga acctgggggt tggcaatcag
     1141 atcgacataa cgctgtcggt atcgggtttc aacgtccgtc agcccatgcc acttttcggg
     1201 aagcggccgg agggctttac tgagaaacgc caggtgatgg acttcgactg taaattcgtt
     1261 cgtctttgta cggaacaatg tccccgtgac gccgatccag tcaccgaggt cgagctgttc
     1321 cacgatggtg taagcctgtt cggagaggag atcttttttg aggtacactt ggaggcggtc
     1381 tgacccgtct tgtagcacag caaacgcagc cttcccgaaa cgccgcaagg cgacgacccg
     1441 tccggcgatc gtgcatgaga ccttttcttg ctccaaggtc tccttggttt tctcaccatg
     1501 caacgcgagt aagtgtccag cccgatcctt gacttcaaag cggctgccgt acggggcgac
     1561 gccggcctct cggaggagct cgagtttctt gatccgctgc tgccgctgtt cgttcagttc
     1621 gtccatcctt acctttcggc aatcctgcta cagatcgtct tccggtccgc cgaccggcga
     1681 gagctgatcg cttcctttcc ccagttttct cttcaaaaag gcttcgatga atccatcgag
     1741 ttccccgtcc atgacggatg agacgttgct gatttgatga cctgtccggt gatctttgac
     1801 cagttggtat ggctggaaca cgtaggagcg aatttggctg ccccacgcga tgtccttctt
     1861 ttccccgacg atggcattga actcggcttc cttcttcttc tgctccactt cgaagaggcg
     1921 agccttcaag attttcatcg ccccatttcg attctggagc tgggagcgct cgttctggca
     1981 ttgcacgacg ataccagtcg gcaagtgcgt aatgcggatg gcagtttcca ccttattgac
     2041 attctgacct cctgcgccgc cagaccgaaa ggtgtcaatc cggagatctt tttcttcgac
     2101 gacgacatcg atgtcctcgc tcagctcagg atacacgaag acggatgcga acgacgtgtg
     2161 ccgccgcttg ttggagtcga acggggaaat gcgcactaag cgatgcacac cggcttccgc
     2221 cttcaagtac ccgtaggcat agggccccat gatcgaaatg gtcacgcttt tgatgcccgc
     2281 ttcgtcgccg ggcaacaggt ccagcgtttc caccttgaat tttttgtgtt ccgcccaccg
     2341 cacatacatc cgaaggagca tttgcgccca atcctgcgat tcagtgccgc cggcaccggg
     2401 atgaatcgcc acaatggcat tattggggtc tagttctccc gagaggagaa gctcgacccg
     2461 caatgtggcg agtcgcggct ccaaatgatt cagttcactg gccagttcgg tctccagggt
     2521 cgagtcgccg ctctcgtggg caagctccaa tagcgcatcc agatcgccca ttttggcctc
     2581 gatgtcgcgc cactgctgaa gctctcgctc aatcgtcgct tttttccggc tcaccactgc
     2641 ggcggcgcgg gcatcgttcc agaagtgagg ctggcccatc tgcgcttcga gttcttgtaa
     2701 gtcggccgtc atgtgagcga agtcaaagat gcccccgtag ttcagagact tgttccgcca
     2761 gggcacgaac gcgtacttct acgtcctcta acatgccatg atcctccttc ttcggtcatg
     2821 ccggtgtgat tgccgtcagg gctggttcct tcgtgcggaa atacccgaac agaatcaaaa
     2881 gcgtgcagat tataacacag gcgtaggcaa acacat
//
LOCUS       DASTWE010000915         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1123641, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000915 DASTWE010000000
VERSION     DASTWE010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123641"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFT30_09535"
     CDS             complement(<1..299)
                     /locus_tag="VFT30_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5092917.1"
                     /translation="MAMRVYELAKKLGMENRVLIPELKKMGASVSSHSSTLDDEIVQK
                     VLDKLGSKSKGQGTGIEGEFGAKSAEHASHGKATAARNHVVEEPPKPDKRRILIKR"
     gene            complement(316..1467)
                     /gene="nusA"
                     /locus_tag="VFT30_09540"
     CDS             complement(316..1467)
                     /gene="nusA"
                     /locus_tag="VFT30_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5092918.1"
                     /translation="MNRELISVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDPKTGEISVVSKKIIVETVSNPKAEISLQEARQYDSEAEVGDEIGSLIEMNEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVTGIILGMERRNYLVDLGKTEAV
                     LPIQEQIPRETYRRGDRVKAMLLEVRRTPKDVQVILTRSHPQFVAKLFELEVPEVMEK
                     IIEIRSVVREPGDRTKIAVTSREKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     QDPRVFIAEALNPATIEKVGIDEEKKSALVVAADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKVERDKEIKAALAEEAEAQRLQEEARQAARAEEATSG"
     gene            complement(1520..2008)
                     /gene="rimP"
                     /locus_tag="VFT30_09545"
     CDS             complement(1520..2008)
                     /gene="rimP"
                     /locus_tag="VFT30_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU5092919.1"
                     /translation="MSIPGNGPQPVADRLHEVITPILWTLGLELVDVVCVGKGPRSIV
                     RVLIDKSGGVSIADCEQAHKALGPALDVADPFPHAYTLEVSSPGLDRPFKRFQDYQRA
                     IGKEVSLKLRQPLDGQWRITGQLMQVDEQAVVLTVAFMRTPQTVKLNRDMIAEAKLVV
                     KI"
     gene            complement(2106..>2697)
                     /locus_tag="VFT30_09550"
     CDS             complement(2106..>2697)
                     /locus_tag="VFT30_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU5092920.1"
                     /translation="YEDAKVYIQLTRGVAPREHSFPSDILPTVVMTIRKFHPLAQELR
                     RAGVSACTREDLRWGRCDIKSVNLLANVLAREEAKKAGVFETILVRDGFVMEGALSNV
                     MAVQDGFVITAPEGPRILSGVTRTLVLELAKKDDIMIEERFIPVDLLYRADEVFLTGT
                     TLEVLGVVQIDGKTIGSGRPGPITKALAARWALLTG"
BASE COUNT          540 a          803 c          650 g          704 t
ORIGIN      
        1 cgtttgatca gaatacggcg cttgtcaggc ttgggcggtt cttcgacaac atggttcctt
       61 gccgccgtgg ctttaccatg gctcgcatgt tccgctgact ttgcaccgaa ctctccttcg
      121 attcctgtcc cctggccttt cgattttgag cccagtttat ccagcacctt ctgaacgatc
      181 tcatcgtcga gtgtactgct atgagaggaa accgatgctc ccatcttctt gagctcgggg
      241 atgagcactc gattttccat tcctaacttc ttcgcgagtt cgtatacacg catagccatg
      301 ggacggttcc gttttctatc cgctcgttgc ttcctcagct ctggcagcct gccgagcttc
      361 ttcttgcagt cgttgagcct cggcttcctc tgcgagcgcc gctttaatct ctttatcgcg
      421 ttctactttt tctttttcgt attccgttgc gctgatgata tcgatcttcc atcccgtcaa
      481 ccgcgccgca agccggacat tctggccatt tttgccgatc gccaacgaca attgcgagtc
      541 ggctgcgacc acgagcgccg acttcttctc ttcgtcgatc cctacctttt caatcgtcgc
      601 agggttcaag gcttctgcaa taaagactcg cgggtcctgg gtccaggtaa tgatatcaat
      661 cttctctccc cgtaactcac gcacgaccgc ctgcacgcga gagcctttaa taccgacaca
      721 ggcccccacc ggatccactg ccttttcacg tgaggtgact gcaatcttcg tccggtctcc
      781 cggctctcga acgaccgatc tgatttcgat gatcttttcc atgacttccg gcacttcgag
      841 ttcaaagagt ttcgccacga attgcggatg actacgggtc agaatgacct gcacatcctt
      901 cggcgtacga cgcacctcca gcaacatcgc cttcacacgg tcgccgcgcc gatatgtttc
      961 acgcggaatc tgttcttgga tcggcaggac ggcctctgtc tttccgaggt ccacaaggta
     1021 attccggcgt tccatgccga gaatgattcc cgtaacgaga tctccttgac gggtcgaata
     1081 ttctttttga accgcttccc attccgcctc acgcaccttc tggaagatga cttgtttcgc
     1141 cgtctgtgcg gcaatccttc ccaattcgtt catttcgatc aacgatccaa tttcgtcgcc
     1201 gacctccgct tcactatcat attggcgagc ttcttgaaga gagatctccg ctttggggtt
     1261 gctgaccgtt tcgacgatga tcttcttgga aacaacggaa atttcaccgg tttttgggtc
     1321 gatctccact tggatgtttt cggcctggcc gaagcgtttc ttcgcagcgg tctgcagggc
     1381 ggattctatg gctccgatga ctcgggcctt gtcaattcct ttttgacgcc cgatttcgtc
     1441 gatcactgag atcagttctc ggttcatacg tcacgctcct gcatccggtt accgcttcta
     1501 atctccgatt ttccagccct taaatcttca cgaccagctt cgcctcagcg atcatgtctc
     1561 gattcaattt caccgtctgg ggtgttcgca taaaagccac cgtcagaacc accgcctgct
     1621 catccacttg catcagttgc ccggtgatcc tccactggcc gtcgagcggt tgccgaagtt
     1681 tgaggctcac ttcttttcca attgcccgtt gatagtcttg aaatctcttg aagggccgat
     1741 cgagacccgg agaagagact tcaagggtat aggcatgagg gaacggatcg gccacgtcga
     1801 gcgccggtcc aagggcttta tgtgcttgct cacagtctgc gatgctgact ccacctgatt
     1861 tatcgatcag aacacgaacg attgagcgag gacctttccc cacacacacc acatcaacca
     1921 actcaagccc gagcgtccat aggatcggtg taatgacttc gtgcaaccgg tcggcaaccg
     1981 gctgcggacc attgcccggt atgctcaacc cattttcctt tgacatgtgt ccaaacaaaa
     2041 aagtgggcat gagcccactt acagaagcag caccataaca tgcacctcag tgcaaagcaa
     2101 gggtgctagc cggtcaacaa cgcccagcga gcggccagcg ctttcgtgat aggacctggc
     2161 cggccggagc cgatggtttt cccatcgatc tgaacaacac cgagtacttc gagtgtggtt
     2221 ccggtcagaa atacctcgtc cgcgcggtaa aggaggtcga ctggtatgaa tcgttcttcg
     2281 atcatgatgt cgtccttctt cgccaactct agaaccaggg tccgggtgac gccggataga
     2341 attcgaggac cttcgggagc cgtgatgacg aacccatcct gaacagccat gacattgctc
     2401 agcgcacctt ccatgacaaa accatctctg accaaaatgg tttcgaagac gccggccttc
     2461 ttcgcttctt cacgggcaag gacgttggcc aggagattga cgcttttgat atcacagcgc
     2521 ccccatcgaa gatcttcccg ggtgcaggca cttacgccgg cgcggcggag ttcttgtgcc
     2581 agaggatgaa actttctgat ggtcatgacg actgtcggga gaatatcgga ggggaaacta
     2641 tgctcacggg gagccacccc tctggtgagc tggatgtaga ccttggcatc ttcatat
//
LOCUS       DASTWE010000916         1436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1188063, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000916 DASTWE010000000
VERSION     DASTWE010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188063"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="mtaB"
                     /locus_tag="VFT30_09555"
     CDS             <1..1284
                     /gene="mtaB"
                     /locus_tag="VFT30_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEU5092921.1"
                     /translation="ETSILGEGLRQKGFELVEFGQPTDLLVLNTCSVTEDAERTSRYL
                     IRKTLKHSPHAFIAVTGCYAQTGMDRLKQQTGIDLIVGHQFKLDLPAYLPPVHELRKR
                     SVAEICHTKTIARGDFDLPHFAESDSTRALLKVQDGCSAMCSFCIIPFARGHERSRTF
                     EDIQREVEVLAARGYREIVLTGVNIGQYAHQGRDFCALLRWLNQAADFERIRISSIEP
                     TTVGEELLDLLASSKKFCPYLHIPLQSGDDQILQAMNRRHTIKSYIKLIEKAHVTIPN
                     LGLGTDLLVGFPGETETAFQNTMAAATDLPFSYLHIFPYSPRPGTAAMRLKQRIPPAS
                     VKKRTDLLLGLDRAKRLAFHNKQIGKTVSVLFEAGTRDAYPFGTTPNFTRVGITVSGD
                     FRNQILPVTITAATDRCTLGHVMSSQQANCAMVVR"
     gene            1281..>1436
                     /locus_tag="VFT30_09560"
     CDS             1281..>1436
                     /locus_tag="VFT30_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092922.1"
                     /translation="MILKTIPQVHIETFGCQMNESDSELVRSMLKREGFVFTEDRERA
                     DVVLVNTC"
BASE COUNT          344 a          411 c          361 g          320 t
ORIGIN      
        1 gaaacctcga tccttggaga ggggctcaga caaaaggggt ttgagctcgt cgagttcggc
       61 caaccgacag atctccttgt gctcaatact tgttcggtta cggaagacgc cgaacggaca
      121 tcgcgatatc tgattcggaa gacactgaaa cactctcccc atgcctttat cgccgtgacg
      181 gggtgttatg cccaaacggg gatggatcgc ctcaaacagc aaacagggat cgatcttatt
      241 gtgggccacc aattcaagct cgatttgccg gcctaccttc ctcccgtcca cgagctacgg
      301 aaacgatccg tcgcggaaat ttgtcacacc aaaacgattg cacgcggaga cttcgatctg
      361 ccgcacttcg cggaatcgga ctccacccgt gcattgctca aagtacagga cggctgcagc
      421 gcgatgtgca gtttctgcat tattccgttt gcacggggtc atgagcgaag cagaactttt
      481 gaggacatcc agcgcgaagt tgaagtctta gccgctcgag gataccgtga aatcgtgcta
      541 accggagtga acatcggcca gtacgcgcac caaggccgcg acttttgtgc gttgcttcgt
      601 tggctcaacc aagcggccga cttcgaacgc atccgcatct cttcaattga acccaccaca
      661 gtcggcgagg aattgcttga tctcttagct tcatcaaaaa aattctgtcc ctatctccat
      721 attccgttgc aaagcgggga cgatcagatt ttgcaagcca tgaatcgacg ccatacgatc
      781 aaaagctaca tcaagctgat tgaaaaggcg cacgtaacca ttcccaacct cggccttgga
      841 actgatctcc tggtaggatt cccgggggag actgagacgg cgtttcagaa tacgatggcc
      901 gccgcgaccg atcttccatt ctcctatctc cacatatttc catactcccc tcgcccggga
      961 acggctgcaa tgcgactgaa acagcggatc ccgccggcat cggtgaagaa acgtaccgat
     1021 cttctgcttg gcctcgaccg agccaaacga ctcgcctttc acaacaaaca aatcggtaag
     1081 acggtctcgg tcctttttga agctgggact cgagatgcct atcccttcgg cacgacgccg
     1141 aacttcacgc gtgtggggat cacagtctcc ggggatttcc ggaatcaaat cttgccggtc
     1201 acgatcacgg ccgcaacaga ccgttgcact ttgggccatg tcatgtcttc tcagcaggca
     1261 aactgtgcca tggtggtgcg atgatcctga aaaccattcc tcaagtccac atcgaaacct
     1321 tcggctgtca aatgaacgaa tccgacagcg aacttgttcg ctcgatgctg aagcgggaag
     1381 gattcgtttt tacggaggat cgtgaacggg ccgacgtcgt attggtcaac acctgc
//
LOCUS       DASTWE010000917         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_347902, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000917 DASTWE010000000
VERSION     DASTWE010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347902"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1416)
                     /gene="der"
                     /locus_tag="VFT30_09565"
     CDS             complement(34..1416)
                     /gene="der"
                     /locus_tag="VFT30_09565"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5092923.1"
                     /translation="MPPLKSAPKQELALPLLPIEGEASPLVAIIGRPNVGKSTLFNKI
                     LGEKIAIVDDVPGVTRDRNYADATYRNRKFRLVDTGGLDPSSSDSMLSLIRRQSELAI
                     AEADILIVLLDGRAGLTPPDHEVVRLLRGVTKPLFYAINKIDTPKSEPLLADFYRLGT
                     DQLYPVSAEHGLGVAELLDAIHPLLPSADEPDDLQQLPRVAVVGRPNVGKSTLVNALL
                     GEERVVVSDIPGTTRDPIDSLVAHHDQCYVFTDTAGIRRRGKIDRGVEGYSVLRSLRA
                     IGRSDIAVLILDGLEGVTEQDTKIAGAILKQGRACILLINKWDLRAGDPKARQEYERE
                     LRRRFPFLTWAPVLYGSALKPESIRRLFPLLKDVHTMFTKRILTGVLNTWLQKILVSH
                     PLPARKHKPSAVTKSAFITQVATKPPVFALFVGHPEDLTSSYLRYLENQLRETYQFTG
                     TPLRLMVRKK"
     gene            1507..>2124
                     /locus_tag="VFT30_09570"
     CDS             1507..>2124
                     /locus_tag="VFT30_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5092924.1"
                     /translation="MTQQLPDWSQIDDVLLDMDGTLLDRHFDNFFFEEELPRRYAALH
                     ALPFETARDRLMAMYRSVEGELAWTDLDYWTKRVGIDVVAMHKELDHMIGFLPGAEEF
                     LRHLRQLGKRVTIVTNAHSTGVSVKVAKTGLDRYVNRIVDAFEVGYLKMRPEYWPNCQ
                     RLLGFDPSRSLFMDDDEGCLMAAKEFGVAHLVHSAKSSSQLPPSPL"
BASE COUNT          443 a          569 c          632 g          480 t
ORIGIN      
        1 ctcatgggta ggatcgttga agggggagtg cggttacttc ttcctcacca tgaggcggag
       61 cggtgtgccg gtgaattgat aggtctcgcg cagttgattc tccaagtacc gcagatacga
      121 agatgtcagg tcttccggat ggccgacaaa caacgcaaag accggcggtt tggtggctac
      181 ctgggtgata aaggcggact tcgtcacggc cgacggcttg tgcttgcgag ccggcaatgg
      241 atggctgact aaaatcttct gcagccaggt attcaatact ccggtaagga tccgcttggt
      301 gaacatagtg tgcacgtctt tgaggagcgg gaagagacgg cggatagatt ccggtttaag
      361 ggctgatcca tacaggaccg gagcccaggt taaaaaggga aaccggcgac ggagttcgcg
      421 ctcgtactct tgtcgcgcct ttggatctcc agcgcgcaag tcccacttgt tgatcaacag
      481 aatgcaggca cgcccttgct tcagaatggc cccggcaatc ttggtgtctt gttccgtgac
      541 tccttccaga ccatcgagta tgaggacagc gatgtcggac cgaccgatgg cacgaagcga
      601 tcggagtacg ctgtagcctt cgaccccgcg atcgatcttg ccgcgccgtc tgatgcctgc
      661 cgtgtcggtg aagacatagc actggtcgtg gtgcgcgacc agtgagtcga tcggatcacg
      721 tgtcgttccc ggaatatcgc tcaccacgac ccgttcctct ccaagcagtg catttacgag
      781 ggtggatttg ccgacgttcg gacgtcccac cacggcaacg cgagggagtt gttgcaagtc
      841 gtcgggctca tccgctgacg gaaggagcgg gtgaatggcg tccaacagct ctgcgacacc
      901 gaggccgtgc tcggcggaca cagggtaaag ctgatcggtg cccaatcggt agaagtcggc
      961 caataacggc tcggacttag gtgtgtcgat cttgttgatc gcatagaaca ggggtttcgt
     1021 cactccgcgc agcagccgca caacttcgtg atccggcggg gtcaagcctg cgcgaccgtc
     1081 caagagcacg ataaggatat ccgcttcagc aatcgccagt tccgactggc gccgaatcaa
     1141 ggacaacatg ctgtcggagg aagatggatc cagtcccccc gtgtcgacca gccgaaactt
     1201 tcgattgcgg taggtggcat cggcatagtt gcggtcacgg gtcacacccg gaacgtcgtc
     1261 cacaatggcg atcttctcgc ccaagatctt gttaaacagc gtcgacttcc ccacgttcgg
     1321 ccggccgatg atggccacaa gaggcgaggc ttcaccttct attggaagca acggcagcgc
     1381 cagttcttgt ttgggagcag atttcagggg tggcataatt gtttattcgt tggaccgtaa
     1441 catagggttt ttctcaaaga caacctgcct cgtcttcttt gcctccgttc ggctatactt
     1501 tgcgtgatga ctcagcaact tcccgactgg tctcaaattg acgatgtgct gctcgatatg
     1561 gacggaacgt tgctcgatcg gcatttcgat aacttctttt tcgaggagga gttgccccgc
     1621 cggtatgcag cgctgcacgc tctcccgttt gagacagctc gcgatcgcct gatggcaatg
     1681 taccgatcgg tcgaaggtga attagcttgg acggacctgg actattggac gaagcgggtc
     1741 ggcatcgatg tagtggcgat gcacaaagaa ctcgatcaca tgatcggctt tctccccggc
     1801 gccgaggaat tcttgcgcca tcttcgacaa cttggaaagc gtgtgaccat cgtgacgaac
     1861 gcccattcga cgggcgtgtc ggtcaaagtg gccaagaccg gtctggatcg gtacgtcaac
     1921 cgtattgtgg acgctttcga ggtcggctat ctcaagatgc gaccggagta ttggccgaat
     1981 tgtcagcgct tattgggttt cgatccgtcg cgatctctgt tcatggatga cgatgaagga
     2041 tgtctgatgg ccgcaaaaga gttcggggtg gcccacctgg ttcacagcgc caagtcaagt
     2101 tcgcaattac cgccgtctcc gctc
//
LOCUS       DASTWE010000918         1470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_897555, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000918 DASTWE010000000
VERSION     DASTWE010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897555"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..903)
                     /locus_tag="VFT30_09575"
     CDS             complement(223..903)
                     /locus_tag="VFT30_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5092925.1"
                     /translation="MKQASTTIVLIDDHEMVRRGLRALLHLEPDMTIVGEAATVADGV
                     AAVERFAPQMVLLDVKLPDASVTEACRRLLAVSPKLRILVLTSYAENSTVMAAVQNGA
                     HGYILKDVRMDDLIRAIRTVAGGQGYLDPRVTQQALHWIRTSSHLGATSHGTSRLSPQ
                     ERLILPLLAEGKTNKEIAVQLRLSDKTVKNYLANIFDKLHVKRRTEAVAWFMKETHVL
                     GAARSQKF"
     gene            complement(900..>1470)
                     /locus_tag="VFT30_09580"
     CDS             complement(900..>1470)
                     /locus_tag="VFT30_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092926.1"
                     /translation="LYAMGLNIETAQRTRSDQTADAKAVDDRMIQQINQLIHEVRGMI
                     RELESGSVQEFDLSSELFALCATYEQTGRLHIKLDLQRSAIDVLTNEEEREILNIVRE
                     AVSNCARHANATRAVVSIRMREAKIRVSIHDDGIGFSTAVGRPRGYGLANMEARAKRL
                     GGALRVQSKAGEGTQVIAEFSLEPLLTSV"
BASE COUNT          317 a          403 c          404 g          346 t
ORIGIN      
        1 tcgagctgtc ggtatctagc ggcgcactca tagagaagag cccggttata ccagatgcaa
       61 ctagacggtc ggccctgtcc cgatcgcaag tggggtcaat catggaccat gtgcttggag
      121 agtagtatat ggcctgaggg tccaccaatg ccagagggta gtcgaggcca gggcgtctta
      181 tgaaggagga agcactacgg ggaataggtt attacaggag cgctaaaatt tttgacttcg
      241 cgcagcaccg agtacatggg tttctttcat gaaccaggca acggcttccg tacggcgctt
      301 gacatgaagt ttatcgaaga tattggccag ataatttttc acggttttgt cgctcaatcg
      361 aagctggacg gcgatctcct tatttgtttt tccttcagcc agaagcggca ggatcaagcg
      421 ctcttgtgga gacagcctgg aggttccatg ggaagtcgct ccaaggtgtg aactggttcg
      481 aatccaatgc agggcttgct gagtcactcg cgggtctaga taaccctggc cgccggcgac
      541 ggtacgaatg gcacgaatga ggtcatccat tcggacatcc ttgagtatgt atccgtgcgc
      601 cccgttctgg actgcggcca tcaccgtgga attttcggcg taactggtga ggacgagaat
      661 ccgaagcttc ggtgaaacgg ccagaagtcg tcgacaggct tcggtgaccg aggcgtcggg
      721 aagcttcacg tcgagcaaca ccatctgagg ggcaaaacgc tcaacggctg ctacgccgtc
      781 cgccacagtg gctgcctccc cgacgatcgt catatccggt tcgaggtgca acagcgcacg
      841 tagtcctcta cggaccatct catggtcgtc aataagaacg atggtggtcg aggcctgctt
      901 catactgaag tcaaaagagg ttccaaggaa aactccgcga tgacttgcgt gccttctccg
      961 gccttggact ggaccctcaa cgccccaccg agtcgtttgg ctcgggcttc catattggca
     1021 agcccatatc ctcgtggccg tccaaccgcc gtcgaaaatc caatgccatc gtcatggatg
     1081 ctcacccgaa ttttcgcttc tcgcatgcga atcgagacga cagcccgggt tgcgttggcg
     1141 tggcgcgcgc agttgctgac agcctcccgc acgatattca ggatttctcg ctcttcctcg
     1201 ttcgtcagta catcaatggc gctgcgttgg agatccagct ttatgtgtaa ccgccctgtt
     1261 tgttcatagg tcgcgcacaa ggcgaagagc tctgacgaca agtcaaattc ttggacactt
     1321 cccgattcca attctcgaat catcccacgg acttcatgaa tcaattgatt gatctgctgg
     1381 atcatccggt cgtcgaccgc ttttgcgtcc gccgtttgat cgctccgagt ccgctgagcc
     1441 gtttcaatgt tcaaccccat ggcgtacaag
//
LOCUS       DASTWE010000919         1182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_218419, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000919 DASTWE010000000
VERSION     DASTWE010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218419"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1182
                     /locus_tag="VFT30_09585"
     CDS             <1..>1182
                     /locus_tag="VFT30_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5092927.1"
                     /translation="IENYERQNAFSEPALRLLTTIAASLGNALENARLFDETQRLLKE
                     TEQRNAELAIINSVQQGLASKLEIRAIYELIGEKVRQTFHADTTYINTYNSDEQAVYS
                     QYYVDKGQRIVRTDPLPFGEGLYTRVIQTRQPLLAGTRQEQIELGVTVASSPGSDQDL
                     NQSYLGVPILLNDQVTGVVSVQSYEQNAFSENDLRLLQTLANSMSVALENAHLFDETQ
                     RLLKETEQRAAELAIINSVQEGLASKLDFQGIIDLVGDKIREIFDAHAINIAEYDAKT
                     DLFSSLYIMERGIRRSFEPMPAGPLFRHILNTRESLRFNTAEEFNNFGAITVPGTEFS
                     MSGIYVPLFQGNKLLGIIALENLDHENAFTESDLRLLTTLANSMSVALENARLFDETQ
                     RL"
BASE COUNT          305 a          339 c          287 g          251 t
ORIGIN      
        1 attgagaatt acgaacgtca gaatgccttt agcgaaccag cactgcgcct cctgaccacc
       61 atcgccgcct cgctggggaa tgccctcgaa aatgcgcgtc tgtttgatga aactcagcgc
      121 ctgctcaagg aaaccgaaca acgaaacgcc gaactcgcca ttatcaacag cgtccagcag
      181 ggactggcat ccaaactgga gatacgagcc atttacgaat tgattggtga aaaggtccgc
      241 cagacttttc atgccgacac cacgtatatc aatacctata acagtgacga gcaggcagtc
      301 tactcacaat attatgtaga caaagggcag cgaatcgtca ggacagatcc cttaccgttc
      361 ggagagggat tgtatacaag agtgattcaa acacgtcaac ctctactggc gggaacgagg
      421 caggaacaaa tcgaattagg cgtaaccgtg gcatcgtctc ccggcagtga ccaggacctg
      481 aaccagagtt acctcggcgt tcccatcctg ctgaatgacc aggtgacagg ggtggtcagc
      541 gtacagtcct atgaacagaa tgcctttagc gaaaatgatc tccgtctcct gcagaccctc
      601 gcgaacagta tgagtgtcgc tctcgaaaat gcccatctat tcgatgagac ccagcgcctg
      661 ctcaaagaga ccgaacagcg cgccgccgag cttgccatca tcaacagcgt gcaggaagga
      721 ctggcatcca aacttgattt tcagggcatc atcgatctgg tcggagacaa gatccgcgag
      781 atcttcgatg cccacgccat caatatcgcg gagtatgatg caaagactga cttgttctcc
      841 tcgctttaca tcatggagcg cggcatccgg cggtcgttcg agcctatgcc ggctggtccc
      901 cttttccggc acatcctaaa tacgcgtgaa tcacttcggt tcaacacagc cgaggaattc
      961 aacaactttg gtgcgatcac ggtcccgggt acggagttta gcatgtcagg gatttatgtc
     1021 cccctgttcc agggcaataa actcctgggt atcatcgccc tcgaaaacct tgatcacgaa
     1081 aatgcgttta ccgagtcaga tttgcgcctt ctgaccacac tcgccaattc catgagcgtc
     1141 gctcttgaga acgcccgcct cttcgacgag acccagcgcc tc
//
LOCUS       DASTWE010000920         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_186269, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000920 DASTWE010000000
VERSION     DASTWE010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186269"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>1029
                     /locus_tag="VFT30_09590"
     CDS             50..>1029
                     /locus_tag="VFT30_09590"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5092928.1"
                     /translation="MPRLLGLLVAATLAANAEADRAVRKNVVVYQEAGRFGGWPANNG
                     VWIWGNEIVVGFMQGYFKDKERGHAIDDDKPEVPRFARSLDGGETWKIETPSFLNGEM
                     KEPEPTDCPGGLDFSAPNSAVSFRMVSSRRGFSRFYYSKDRAKTWQGPYKLPTFDRKG
                     IAARTDYLVDGKNQLTAFITASKENGREGRVLCIRTTDGGKTWSLVSWVGPEPEGFAI
                     MPSSVRLSPSRILTTVRRKEGDQHFIDGWVTDDNGATWKLLGKAVESTGGSVGNPPSM
                     IQLKDGRLALTYGFRSEPYGIRARLSRDGGNSWGSEIVLRDDAGTWDLGYP"
BASE COUNT          230 a          270 c          317 g          212 t
ORIGIN      
        1 ggatcgacgg cgcgaggtga cttgggacga gatgatgaag gcgtgaccaa tgccaaggtt
       61 actgggccta ctggttgccg ccacattggc tgcgaacgcc gaggcagatc gcgcggttcg
      121 gaaaaatgtc gtggtctacc aggaagcagg gcgttttggc ggctggccgg ccaacaatgg
      181 tgtctggatt tggggaaacg aaattgtcgt cggattcatg cagggttact tcaaagacaa
      241 ggagcgtggc cacgcgattg atgacgacaa accggaagta cctcggttcg ctcgtagcct
      301 ggacggcggc gaaacctgga agatcgagac accatcgttt ttgaacggcg agatgaagga
      361 gccggagcct accgactgcc ccggcggcct cgatttttcc gctcccaact cggcggtcag
      421 ttttcgaatg gtctccagcc gccgtggatt ctcacggttc tactactcca aagaccgggc
      481 taagacctgg caaggtccat ataagctacc gacgttcgat cgaaaaggaa tcgccgctcg
      541 aaccgactat cttgtcgatg gaaagaacca actcacggct ttcatcaccg cttccaagga
      601 gaacggccgc gaagggcgag tgctctgcat tcgcaccacg gacggtggca agacatggag
      661 tcttgtttcc tgggttggac cggaacccga aggtttcgca atcatgcctt catctgtccg
      721 actgtcacct tcacgaattc tgacaaccgt tcgccggaag gaaggagatc agcacttcat
      781 cgatggctgg gtaacggatg acaacggtgc aacgtggaag ttgctgggta aagccgtgga
      841 atccacgggt ggctcggtgg gcaatcctcc cagcatgatc cagttgaagg acggccgttt
      901 ggcgctgact tatggatttc gctctgaacc gtatggcatc cgcgcgcgcc taagccgtga
      961 cggtggcaat agctggggca gcgaaattgt ccttcgagac gatgcgggaa cctgggatct
     1021 cggttatcc
//
LOCUS       DASTWE010000921         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1479038, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000921 DASTWE010000000
VERSION     DASTWE010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479038"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..>1065)
                     /locus_tag="VFT30_09595"
     CDS             complement(242..>1065)
                     /locus_tag="VFT30_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092929.1"
                     /translation="LGMELGSHTVAHSKGLDTFPLGTGTERYPSYRPYVKSRQIAYNG
                     TVLGELRVSKFLIEHFSSPTSVISFRPGNLSNPLTLPQALVASGYRFSSSATANNSLT
                     HLPYQLTYDRGVESEVGIFEFPVTLEDEELPELGSRLPEALELVRQVGRYGGSFVVLI
                     HPNILGHKLEFERKFVAAVRDRAWFGSVGEFGQWWAARNQVEIDVHQDGSFHTVSLDI
                     PDKIAGLDIQIPSEWILKQQDPVTLTVASDGGHVILQEAAGPVKLVFESASEIKP"
BASE COUNT          233 a          284 c          291 g          257 t
ORIGIN      
        1 ccggcctgaa gccgccggga tcgcgctcat cctcaagggc tggctcaggg aaggtacatc
       61 tgaggcgaaa attgagtgta aaggcatcgc cctatttgaa ggattgtttt cggccttgtc
      121 ccgcgatggt cgatcttcca aagctagata gttatggctt cagaccagtc tactgttgca
      181 ggccaattgc cttgcaactc cccttatatc gcgccatgcg cagtttatct gaatggtacg
      241 atcagggttt aatttctgat gcggattcaa agaccagctt gaccggccca gccgcttctt
      301 gaagaataac gtgcccgcca tcggaggcta cggtgagcgt caccgggtct tgttgtttca
      361 ggatccactc gctgggtatc tgtatatcca gaccggcaat tttgtccgga atgtcgagcg
      421 atacggtgtg aaacgacccg tcctgatgca catcgatttc cacctggtta cgggctgccc
      481 accactggcc gaattctccg acagatccga accaagcacg atcgcggacc gccgccacaa
      541 actttcgttc gaactcgagc ttgtgtccca atatattggg gtgaatcagg acgacaaagc
      601 tgcccccgta gcgaccgacc tgccggacca attcaagggc ctctggaagt cgggacccca
      661 gttcgggtaa ctcctcatct tcgagcgtca ccggaaattc gaagatgccg acttcgctct
      721 ccacccctct gtcataggtc aattggtacg gcaggtgcgt gagcgagtta ttggccgtgg
      781 cggacgaact gaatcgatag ccgctggcta ctagagcttg tggcaatgtg agaggattcg
      841 acaggttgcc tggtcgaaag gagatgacgc tagttgggga agaaaagtgc tcgatgagga
      901 atttgctcac tctcaattct cccaataccg tgccgttgta ggcgatttgt cgtgacttca
      961 catagggtct gtatgaggga taacgttctg ttccggtgcc caatggaaat gtatctaggc
     1021 ccttcgagtg agccacggtg tggctaccta actccatgcc cagtt
//
LOCUS       DASTWE010000922         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_541990, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000922 DASTWE010000000
VERSION     DASTWE010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541990"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFT30_09600"
     CDS             complement(<1..553)
                     /locus_tag="VFT30_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU5092930.1"
                     /translation="MENKVVLVGSIIFVFTSFFLMIGLLAYESYKAKQVKQLAASVKS
                     EARPTASSLPAQDFSMYKTKIGDEGREMVQIPEGPFTMGSNDGDPDEAPEHQVYLKGF
                     YLDRKEVSQEEYMRFAKMTKRAMPRIEVFDDDQSKILKPEFAAMSVSWDEAAAYCKWA
                     GKRLPTEAEWEKAGRGESKRRYPW"
     gene            complement(579..941)
                     /locus_tag="VFT30_09605"
     CDS             complement(579..941)
                     /locus_tag="VFT30_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092931.1"
                     /translation="MTRGKAFDIALGIAVMGTVGTLIGLTMGGGLMPVAISVGLGLGV
                     VIGFLGGRRFLVSILVGTMLGGVLAWLIAGVERIWVGAGAGAAKGGFLGVQISMLLDV
                     RASKKAASEQVETSSSYR"
     gene            complement(975..>1663)
                     /locus_tag="VFT30_09610"
     CDS             complement(975..>1663)
                     /locus_tag="VFT30_09610"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEU5092932.1"
                     /translation="VARNIRKGMKDMTRRVLLCTAAILGILVGVFAWQGPVVAQGQAL
                     VLEDFQAKEADGFPSLWDHENQRSQSKGREAYKVQSENGTNFLSAKDAGQRIKKKKID
                     WDPKAYPVLTWRWRLNKATTGNEPLAAVYASLDTDLLFIPVFTKYVWSESKPDGTLTE
                     GGMFSGSEIVVQTGTKEVGQWFEERVNVYEDFKRIHKHEPAEKAWGISIIAAPGVEVD
                     FGPLIAVPAK"
BASE COUNT          385 a          522 c          379 g          377 t
ORIGIN      
        1 cccacggata ccttctcttg ctctctccgc ggccagcctt ctcccactcg gcttcagtgg
       61 gaagtcgctt gcctgcccac ttgcagtagg ctgccgcttc gtcccaggag acgctcatgg
      121 cggcaaattc cggcttcaaa atcttcgact gatcatcgtc gaacacctcg atcctgggca
      181 tggcgcgctt ggtcattttc gcgaaccgca tgtattcctc ttgggagact tcttttcggt
      241 cgagatagaa ccccttcagg tagacctgat gctcaggagc ttcatccgga tcaccgtcat
      301 tgcttcccat tgtaaaggga ccttccggga tctgcaccat ttcccgacct tcatctccga
      361 tttttgtctt gtacatcgag aaatcctgag ccggcaggct acttgcggtc ggtcgggctt
      421 ccgacttcac cgatgcggca agctgtttca cttgcttggc cttataggat tcataggcca
      481 gcaggccgat catgagaaag aacgacgtaa acacaaaaat gatggagccg actaggacaa
      541 ccttgttttc cacaaacacc tcttctaatc gagagtacct accgatagga ggacgacgtc
      601 tcaacctgtt cggacgccgc cttcttggaa gcacgcacat cgagcaacat tgaaatctgg
      661 acacccagaa agccaccctt ggcagcaccg gctccggcgc cgacccagat ccgctcgaca
      721 ccggctatca gccaggctaa gacacctccc agcatagtac cgacaaggat actcacaaga
      781 aatcggcggc cgccgagaaa gccgatcaca acaccgagcc caagcccgac agaaatggca
      841 accggcatca gccccccgcc catggtcagc ccgataagag taccaacagt acccatcacg
      901 gctattccga gtgcaatatc aaacgccttg cctctcgtca tcactgatcc ttcatggact
      961 ggagagactg atctctactt ggccggaaca gcaatcagcg gaccaaaatc gacttcaaca
     1021 ccgggcgccg caatgataga gattccccat gccttctccg ccggctcatg cttgtgaatc
     1081 cgcttgaagt cctcatacac attcacccgt tcctcaaacc attgcccgac ttccttcgtt
     1141 ccagtctgga cgacaatctc ggagccacta aacatcccgc cttccgttaa ggtgccatcc
     1201 ggtttcgact cgctccatac atatttcgtg aaaaccggga tgaagaggag atcggtatcg
     1261 agggaagcat agaccgcggc aagcggctca tttccagtcg tcgctttatt gagacgccat
     1321 cgccaggtca gaacaggata ggccttggga tcccaatcga ttttcttttt cttgatccgc
     1381 tgcccggcat ctttcgccga cagaaagttc gttccgttct ccgactgaac cttataggct
     1441 tcgcgacctt tcgattgact tcgctgattt tcgtgatccc acaaagaagg aaacccgtcc
     1501 gcttccttcg cttgaaaatc ttccagcacc agggcttgcc cttgggcgac aacgggacct
     1561 tgccaggcaa aaacaccgac tagaatgccg agaatggctg cggtacaaag cagaacccga
     1621 cgcgtcatat ccttcattcc ctttcttatg tttcttgcga cgg
//
LOCUS       DASTWE010000923         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_24990, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000923 DASTWE010000000
VERSION     DASTWE010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24990"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFT30_09615"
     CDS             complement(<1..640)
                     /locus_tag="VFT30_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5092933.1"
                     /translation="MNSSTRITQQFSLRTRNFTKRVNVSTSPGLAWVTPILTVWLVAA
                     FATTMALGQSYYGGLRGVVRDPDGSVVANAKVTLVDQATRLERLTYSSGEGVYYFNQI
                     VPGTYSISTEAAGFKKFEQRDVIIATQQQVSLDLALQIGEITQTVEVRTEVPLIEYSN
                     ASQGQVLDNKQLRELPNFGRNPYGMSRITQNVTPVGNPATNNMQTQSATALTT"
     gene            complement(737..>1234)
                     /locus_tag="VFT30_09620"
     CDS             complement(737..>1234)
                     /locus_tag="VFT30_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5092934.1"
                     /translation="SARGTMSQELIGVISDTHGQFDPKIPRVFKGVSHILHAGDFGDL
                     RVLRQLEQIAPITAVWGNVDEGNVPPGVNEMQTLQFDGLRVFMTHILGSPRRLTARLK
                     QRIEQVQPDVVIFGHSHTPLIERLGRVLYFNPGSAGPKRFSLQRSVGLLRCQNGEPQG
                     EVIYL"
BASE COUNT          259 a          335 c          337 g          303 t
ORIGIN      
        1 cagtcgtcag agccgtggca ctctgggtct gcatgttgtt ggtcgccgga ttgccaacag
       61 gcgttacgtt ttgggtaatc ctcgacattc cgtacggatt ccgcccgaag ttcggaagct
      121 ccctcaattg cttgttgtcc aaaacctgtc cctgggaagc attcgagtat tcgatcagtg
      181 gcacttccgt tctcacttcc acggtttgag tgatttcgcc gatctgaagc gcgaggtcga
      241 gagacacctg ttgttgagtg gcgatgatga cgtctctctg ctcgaatttc ttgaatccgg
      301 cagcctcggt gctgatggag taggttccgg gaactatttg gttgaagtag taaacgcctt
      361 caccgctgga atacgtaagg cgttcgaggc gcgtcgcctg atccaccagc gtcaccttgg
      421 cgttcgcgac gacacttcca tctgggtcgc gaaccacgcc tcgtagtccc ccgtagtagg
      481 actggcccag cgccatcgtc gtagcgaagg cagcaacaag ccaaaccgtc aaaattggcg
      541 tcacccaagc caaaccgggg ctggttgaga cattcactct cttcgtgaag ttgcgcgtgc
      601 gcagagaaaa ttgctgagtg atacgcgtcg aactattcat acccggctct cctgtactga
      661 acaaagttca tttgaatggc ggaaacttat acctgttgtg tacaggtgtc aatctcgaga
      721 atgggcaacc tcgagctcaa aggtagatga cctccccttg gggctctcca ttctgacatc
      781 tcagcagacc cactgagcgc tgaagtgaga atctctttgg tccggcactg cctgggttga
      841 aatacaacac tcttccgagt ctttcaatca gcggcgtgtg gctgtgacca aatatgacca
      901 cgtcgggctg cacttgctca attcgttgct tgagccttgc ggtaagtcgc cttgggctgc
      961 cgagaatgtg ggtcatgaag acgcgcaggc catcgaattg gagggtctgc atctcgttca
     1021 cacccggagg gacattgcct tcatcgacat tgccccaaac ggctgttatg ggggcaatct
     1081 gttcaagttg tcgaagcacc cgcagatcac caaagtctcc cgcgtggagg atgtggctca
     1141 cgcctttgaa cacgcgggga atcttgggat caaactgccc gtgtgtgtcg gaaatgactc
     1201 cgatgagttc ttgactcatg gtccccctcg ctga
//
LOCUS       DASTWE010000924         1264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_57142, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000924 DASTWE010000000
VERSION     DASTWE010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57142"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFT30_09625"
     CDS             complement(<1..582)
                     /locus_tag="VFT30_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEU5092935.1"
                     /translation="MVLEWLKNLLIVALVFVPLERVFALHPRQKLFRRGWLNDAIYLT
                     VNGQVINLALTLIVAGIIFAANWTMPAAVKTAVSGQPYWLQVIEVVVLSDLGFYAAHR
                     TFHSVPRLWKFHAIHHSIEELDWLAGARVHPIDQIITKGVSFLPVFSLGFSDIAIGIY
                     LTIYSWQSFLVHSNLAIKFGPFRWLLASPEFHHW"
     gene            complement(584..>1264)
                     /locus_tag="VFT30_09630"
     CDS             complement(584..>1264)
                     /locus_tag="VFT30_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5092936.1"
                     /translation="ASGAVSMSSDSNKIRLVIADDHPIVLEGIARALEAEDGFEVHDK
                     CGNGADALDAIRRHRPDVAVLDIAMPGVSGLDALASINAENLETKIVFLTANATDTHI
                     LSLIERGAKGLLLKDGPISELAQCIRTVARGGRQFPQDIVTSAIERETGRRSIHEQLE
                     RVLSPREREVMLLVADGLPNKEIARRLNVSEGTVRIHVHNIYQKTGIGSRATLIALTL
                     TCRDLLRP"
BASE COUNT          286 a          367 c          357 g          254 t
ORIGIN      
        1 ccaatgatgg aattccggtg aagccagcag ccaacggaag gggccgaatt tgatcgcgag
       61 atttgaatgg accaagaacg actgccagct atagattgta aggtaaattc ctattgctat
      121 gtccgaaaac cccaatgaga agaccggcaa gaacgagaca cctttcgtga tgatttgatc
      181 tatcgggtga acccgggctc cggccagcca gtcgagttct tcaatgctgt ggtgaatcgc
      241 atggaacttc caaaggcgcg ggaccgaatg gaaagttcga tgcgcagcat agaaaccaag
      301 atcggacaag acgacgacct cgataacctg caaccaatag ggctggccgg aaacggccgt
      361 tttgacggcg gccggcatgg tccaattcgc cgcgaagatg attccggcca cgatcaaggt
      421 cagagccagg ttgatgacct ggccattgac cgtcaggtag atcgcatcgt tgagccaccc
      481 gcgccggaac agcttttgcc gtgggtgcaa agcgaagacc cgctcaagcg gaacgaatac
      541 cagagcgacg atcagcaggt tcttaagcca ttccagaacc atgtcagggc ctcaacaaat
      601 cccggcaagt cagtgtcagc gcgatcaggg tagcccggct tccgatcccg gtcttttgat
      661 agatattgtg aacgtgaatt ctgaccgtac cctcggagac attcaggcgc ctggctattt
      721 ccttgtttgg caacccgtcg gcgacgagaa gcatgacctc gcgttcccgc ggcgacaaga
      781 cgcgttcgag ctgttcatgg atcgagcgcc gccctgtctc cctctctatc gccgaggtga
      841 ctatatcttg ggggaactgc cgtccgccgc gcgcgaccgt acggatgcac tgggctagct
      901 cgctgatagg cccatccttc aggagcagcc ccttcgcccc gcgctcgatc agcgacagga
      961 tatgggtatc ggtggcattg gcggtaagaa agacgatctt ggtttcaagg ttctcggcgt
     1021 tgatggaagc cagcgcgtcc agcccgctca ctcccggcat cgcgatatcc agcaccgcga
     1081 catcgggacg gtgccgtctt atcgcgtcca atgcgtccgc gccgttgccg cacttgtcat
     1141 gaacctcgaa accgtcttcc gcctcgaggg ctctcgcgat accttccagc acgatggggt
     1201 gatcgtctgc aatcaccagc cggattttgt tcgaatccga actcatggag acagctccag
     1261 acgc
//
LOCUS       DASTWE010000925         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_671318, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000925 DASTWE010000000
VERSION     DASTWE010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671318"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFT30_09635"
     CDS             complement(<1..1150)
                     /locus_tag="VFT30_09635"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEU5092937.1"
                     /translation="MKLNTSMHVTHVSDEVWEIAPAEKEGMLVPARIYATPTILQAMD
                     AGVFEQVTNVACLPGIKRYALCMPDGHWGYGFPIGGVAAFDARSGVISPGGVGYDVNC
                     GMRLIRTDLTRAEVQPRLDRLMTELFRRVPAGVGASGFVRLDRSSFEQVMTDGARWCI
                     EQGFGWHRDLEAIEEGGCIIGVDPSKVSDQAVSRGINQLGTLGSGNHYLEVQVVSSDR
                     VFDRETASAWGITGHDQIVVMVHCGSRGFGHQIASDYLKVFERAMRRYGISVRDQQLA
                     CAPFTSVEGQDYFAAMNCAANTAFANRQVITHQIREAFAAVFGRSAEEMGMELVYDVA
                     HNIAKVERYPEGELVVHRKGSTRALGPGSPELPPRYRTTGQPVICGGSM"
     gene            complement(1262..1699)
                     /locus_tag="VFT30_09640"
     CDS             complement(1262..1699)
                     /locus_tag="VFT30_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEU5092938.1"
                     /translation="MSHGFHFLDDVATADLAFDASGDSLQELFQGATCAVIEALADPT
                     TVGSTWRQTIERSDENPAELLFDWLSDLVYWKDAAGVVFNRSELMLIRQDHGCWKLTG
                     LLFGEPVNGSAQTLRADVKGVTKHLYRLEQSGGQWIVRVVLDV"
     gene            complement(1770..>2105)
                     /locus_tag="VFT30_09645"
     CDS             complement(1770..>2105)
                     /locus_tag="VFT30_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5092939.1"
                     /translation="DAIGKIKDGADGMKIHQGICARFEKAGYKTGLLNGRMQGYFHGT
                     GHGVGLDIHEAPRISRTGSLLQEGHVVTVEPGLYYPGLGAVRIEDMVLVTKDGCRNLT
                     NFSKVFELG"
BASE COUNT          499 a          625 c          494 g          487 t
ORIGIN      
        1 ccattgaacc gccacaaatg accggttggc cagtcgtacg ataccgtggc gggagctcgg
       61 gactccccgg ccccaatgct cttgttgaac ctttccgatg gaccaccaat tctccttccg
      121 gataccgctc aactttggcg atattgtgcg ccacgtcgta gaccagctcc atacccatct
      181 cctctgccga tcgaccgaac acagcagcga aggcttcgcg gatctgatga gtaatcactt
      241 ggcgattggc aaaggcggtg ttggccgcac agttcatggc cgcaaaatag tcctgtcctt
      301 cgactgaggt aaacggagca caggctaatt gctggtccct cacggaaatt ccatatcgtc
      361 gcatcgctcg ttcgaacacc ttgagatagt cacttgctat ctgatgaccg aatccgcgag
      421 atccgcagtg aaccatcacg actatctggt cgtgaccggt gatcccccat gctgaagcag
      481 tctccctgtc aaatacccga tcgcttgata ccacttgtac ctcgagatag tggttccctg
      541 accccaaggt ccccaactga ttgattcctc gactgactgc ctggtcgctg acttttgaag
      601 ggtcgacgcc gattatacat cctccttctt caattgcctc cagatcgcga tgccaaccga
      661 atccctgctc gatacaccat cgagccccgt cggtcatgac ttgttcaaat gaggagcggt
      721 ccaaccgtac aaaaccgctt gcgcctaccc ctgccggcac ccggcgaaac aattccgtca
      781 tcaatcgatc aagacgtggc tgcacctctg cccgggtgag atctgtcctg atgagacgca
      841 tcccacaatt gacgtcatag ccgacaccac caggcgaaat gactcctgaa cgggcgtcaa
      901 atgcggcgac gcctccaatg ggaaaaccat aaccccaatg gccatccggc atgcagagcg
      961 catatcgttt aattccagga agacaagcga cattcgtcac ttgctcgaac actccggcgt
     1021 ccatcgcttg aagaatggtc ggcgtcgcat agatccgtgc aggaacaagc atcccttctt
     1081 tttcagctgg agcgatctcc cagacttcgt cggaaacgtg cgtgacatgc atggaagtgt
     1141 tcagtttcat cgcaatcccc agtcaatggt cataggtcat tgacgaagcg caaatcatct
     1201 cagttaatgt aatgtcggtt cttcgtattt ttaactattg accagtaatt aatgactcta
     1261 gtcaaacatc caacaccact cgaacaatcc attgcccacc cgattgttca agacgataga
     1321 gatgtttagt gactcccttc acatcggccc tcaatgtctg ggccgatcca ttgaccggtt
     1381 ctccgaacaa taaccccgtc agtttccagc agccgtggtc ttggcggatc agcataagtt
     1441 ccgaccgatt aaatactact ccggctgcat ccttccagta cacgaggtcc gagagccaat
     1501 cgaacaacag ttcggcagga ttctcgtctg atcgttcaat cgtttgccgc caggtggacc
     1561 ccactgttgt aggatcggcc aaggcttcga tgaccgcaca tgtcgcccct tggaagagct
     1621 cttggagtga atcaccggag gcgtcgaacg ccaggtcagc tgtggcgaca tcgtccagaa
     1681 agtgaaatcc gtgggacatc cctgtgccaa agcgaaatgt tcagccgatg gaaaaatgca
     1741 gagatgagaa gcccggtccg gactgccgtc tatccgagct caaagacttt agaaaaattg
     1801 gtgagattac ggcagccgtc tttcgtgacc agtaccatgt cctcgatccg gactgctccc
     1861 aatcctggat aatacagtcc tggctcgaca gtgacgacgt gaccctcttg gagaagtgaa
     1921 ccggttcgac taatacgtgg cgcttcgtga atatccagcc ccacaccatg tccggttcca
     1981 tgaaaatacc cttgcatgcg cccgttcagc aggccggtct tatatccagc cttttcaaaa
     2041 cgcgcacaga tgccttgatg gattttcata ccgtcagctc cgtctttgat tttgccgatg
     2101 gcatc
//
LOCUS       DASTWE010000926         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_897782, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000926 DASTWE010000000
VERSION     DASTWE010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897782"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..>1197
                     /locus_tag="VFT30_09650"
     CDS             121..>1197
                     /locus_tag="VFT30_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5092940.1"
                     /translation="MRKLCRVLGTAGLLCSISPNALAQSLPEAHVPGRLLVRFRESVS
                     PDQVQNVLQSVQARSTKQIAGIGVHVLELPANASETAYQNILRNRPEVDFADLDIVRT
                     HEQSQITPNDPQFSSEWHLSKIQAPAAWSTTTGSPSIIIAIADTGVDSNHPDLMTKMV
                     AGWNVNDNNSDTRDVYGHGTMVAGTAAAASNNGVGVASVAWNSWLMPVRVSLSNGSAY
                     DSAIASGITWAADHGAKVVNVSYKVTGSSTVSSAAKYFYSKGGIVTISAGNYSTYESI
                     PDNPYIVTVGGTDPSDLLYSWSNTGTHIDVVAPGCVTTTSNGGGYAGGCGTSFSAPIA
                     AGVAALVMSAKPGLSPAEITRVLQS"
BASE COUNT          283 a          303 c          322 g          289 t
ORIGIN      
        1 ttaagtaatt ttctgtattc gctttcattg gccagcctag tagtatgaag caaaccgcag
       61 tactaagtac ctgaaagtgt gcgacgtgag acggatgttc tcatgcgagg tgtgtaacga
      121 atgcgtaagc tgtgtcgcgt cctcggaaca gcaggacttc tctgttccat ttctcccaat
      181 gctctcgccc aatctttacc agaggctcat gttcctggac gacttcttgt ccggttccgg
      241 gaatcggtct ctccagacca agtgcaaaat gtgcttcagt cggtgcaggc acgtagtacg
      301 aagcagattg cgggcatcgg cgtccatgtg ctcgaactac ctgccaacgc ttcggaaact
      361 gcgtatcaga acattctacg caaccgtccg gaggtggact tcgctgatct cgatatagtt
      421 cggacgcacg aacagtctca gattaccccg aacgatccgc aattttctag cgaatggcac
      481 ctatcaaaaa ttcaggcacc ggcggcttgg tctacaacaa ccggcagccc gagcatcatc
      541 attgccatcg ccgacacggg cgttgattcc aatcatccag acctcatgac gaagatggtc
      601 gcgggttgga atgtaaacga taataacagc gatactcgag acgtatatgg acatggcacg
      661 atggtcgccg gcacggctgc agctgctagc aacaacgggg taggtgtcgc ctctgttgct
      721 tggaattcct ggcttatgcc tgtacgcgtt tcattgagta atggaagcgc ctacgattca
      781 gctattgcaa gcggcattac ctgggctgcc gaccatggcg caaaagttgt gaatgtgagc
      841 tataaagtga cgggtagtag tacggtgagt tccgcggcga aatattttta cagtaaaggt
      901 ggaatcgtca cgatatcagc agggaactac agcacctatg aatcgattcc agataacccg
      961 tatatcgtca ccgtaggcgg cacagatccg agcgacttac tatactcctg gtcgaatacg
     1021 ggaacgcata tcgatgtggt cgcacctggt tgtgtaacta cgacgtcgaa tggaggtggt
     1081 tacgccggtg gttgtggaac ttccttctct gctccgatcg cagccggggt ggcagctctg
     1141 gtgatgtcgg ctaagcctgg actttcgccg gcagaaatca cgcgtgtact ccagagt
//
LOCUS       DASTWE010000927         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1479282, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000927 DASTWE010000000
VERSION     DASTWE010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479282"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..317)
                     /gene="thiS"
                     /locus_tag="VFT30_09655"
     CDS             complement(117..317)
                     /gene="thiS"
                     /locus_tag="VFT30_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU5092941.1"
                     /translation="MQVKINGKPEEIQSGTVLDLLKTKNIEPQMVAVEVNNKVLDRDH
                     LATTHLNEGDHVEFLFYMGGGR"
     gene            472..891
                     /locus_tag="VFT30_09660"
     CDS             472..891
                     /locus_tag="VFT30_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092942.1"
                     /translation="MLDFDRTRRHYAACMWKKNFLFRATESTPLTESENELFHDTEPA
                     LDSAGLVLDKFLSVWVQGEGTEEKPSTFTNLYVRTAMLDVRKHVSLLQPLQGRTHQIK
                     QLLTKEQKQFLQQWLRVHAPQAWESSEDHFRDLFEQE"
     gene            888..1454
                     /locus_tag="VFT30_09665"
     CDS             888..1454
                     /locus_tag="VFT30_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5092943.1"
                     /translation="MIRPSPFRLSTQRCRGFIIWLLAASLLGPSLEVSAASVEVWYGP
                     EDRPLEQLVRMYDRATRYIFVAVYGLTSPLTVKALVDAKRRGVDVRVVTDRERLEDMK
                     QQTALSTLREAGIPIKINRHDGLMHLKQAVIDDEINTNGSMNQTTSGNRYNDERLDII
                     RDHAIAVKAREKFLSLWKDHERFQEWKK"
     gene            1562..>2143
                     /locus_tag="VFT30_09670"
     CDS             1562..>2143
                     /locus_tag="VFT30_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU5092944.1"
                     /translation="MTEETDIAVVGAGGGGAVLALALAQKGIKTIVFEQAPGPPQGLR
                     GEILQPNGQQVLDRLGLLSRLPLESTRTVHKFHFCRVGGERLCTVDYSERPPPYNRAV
                     VTLPNVAHHAILSAIERESSVSLRYRSAFTGLLRENGRVVGLTAKQGDDHVTVKAKVV
                     IGADGAFSKVRDALQIPADLHLYPHGYLIAMLDA"
BASE COUNT          485 a          543 c          628 g          487 t
ORIGIN      
        1 gttgaaaaac tcgacctttc cgtaaatcgt tgccgagcca ggttttgaga gacggttcaa
       61 ccggacaagc ggagtgttgc cgatcagctc ggtgatatct ttgtgcggcg tcgtactcac
      121 cggccccctc ccatataaaa gaggaactcc acgtggtctc cttcgttgag gtgggtcgtg
      181 gcaaggtggt cgcgatccag caccttattg ttgacctcga cggcgaccat ctgcggctcg
      241 atattctttg tcttcagcaa atcgaggacg gttccgctct ggatctcctc aggctttcca
      301 ttgatcttga cctgcacaga ttctcccaag ttggcttaga gattaaagaa ttttgaagct
      361 agcaaaggca tgctctgctt gtcaaggcta cttcattggc gtgagagaca cgaaagctca
      421 acaggattca tgcccaacgg tatctgctga gaggagattg tcctaagagg gttgcttgac
      481 tttgatcgga ctcgccgcca ctatgccgcc tgcatgtgga aaaagaactt cctctttcgc
      541 gctactgaat cgacaccgct gaccgagtcc gagaacgagc tgttccatga tacggaaccg
      601 gcgctggata gtgcaggtct tgtcctggac aagttcctct cggtgtgggt gcagggcgaa
      661 gggacggagg agaagccgtc gacctttacg aatctctatg tccgtaccgc tatgctggat
      721 gtgaggaaac acgtgagtct gctccagccg cttcaagggc gcacacatca gatcaagcaa
      781 ctgctcacca aggagcagaa acagtttctc caacaatggc ttcgggtgca tgccccacag
      841 gcctgggagt cgtctgaaga tcactttcgc gacctcttcg agcaggaatg atcaggcctt
      901 cgccgttccg attatccacg cagcgctgcc gagggtttat catctggcta cttgccgcaa
      961 gtctgcttgg gccaagcttg gaggtctcag cggcatcagt tgaagtctgg tatggcccgg
     1021 aggataggcc gcttgaacag cttgtacgga tgtatgatcg tgccacacga tacatcttcg
     1081 tcgccgtata tgggttgacg tcgccgctta cggtgaaggc gttggtcgac gcgaaacggc
     1141 gtggcgtgga tgtccgtgtc gtgacggatc gcgaacgact tgaggatatg aagcagcaga
     1201 ctgccctctc aacgttgcgc gaagccggga ttccgatcaa gatcaatcgg cacgatggtt
     1261 tgatgcattt gaaacaagca gtgatcgacg acgagatcaa taccaacggg tccatgaatc
     1321 agacgaccag tggaaaccgc tacaatgatg agcggctgga tatcatcagg gatcatgcca
     1381 ttgccgtcaa agcgcgtgaa aagtttcttt cgctctggaa agatcacgag cgattccagg
     1441 agtggaaaaa gtaggaatgt tgaggttaag gttgaggtta aggccaaggg tcagaagatt
     1501 ctagtggaag agcgagaacg ctcccagact caaccttggc cttagccttt ggagtcgagt
     1561 ggtgaccgaa gaaaccgaca tcgccgtcgt tggggccggt ggaggaggtg ccgtactggc
     1621 tcttgcactg gcccagaagg ggatcaaaac gatcgtcttc gagcaggcac caggaccgcc
     1681 tcagggattg cgcggagaga tcctgcagcc gaacggccaa caagtgctcg atcgcttggg
     1741 actcctgagt cgattgccgc tcgagtctac ccgtacggtg cataagtttc atttttgccg
     1801 tgttggtggc gagcggctgt gcaccgtgga ctatagtgaa cgacctccgc cgtacaatcg
     1861 agccgtggtc acattgccga atgtggcgca ccatgccatt ctgagtgcaa ttgagcgaga
     1921 atcatccgtc tcattgcggt atcgatcagc attcacaggt ctgcttcgag agaatgggcg
     1981 ggtcgttggt ctgaccgcaa agcaagggga cgaccatgtg actgtcaaag caaaggtggt
     2041 catcggcgcc gatggggcgt tctcgaaagt tcgggacgcg ttgcagatcc cggccgatct
     2101 acatctatat ccgcacgggt atttgatcgc catgttggac gcg
//
LOCUS       DASTWE010000928         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_962198, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000928 DASTWE010000000
VERSION     DASTWE010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962198"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /gene="topA"
                     /locus_tag="VFT30_09675"
     CDS             complement(<1..1926)
                     /gene="topA"
                     /locus_tag="VFT30_09675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5092945.1"
                     /translation="MGKSLIIVESPTKARTITKYLGRGYTVMASVGHIKDLPTSKLGV
                     DLENDFEPQYVTIKGKSKVLAEIKKKAEEADKIFLAPDPDREGEAIAWHIEQELLGKS
                     KKKKKDGKIFRVLFNEITESAIKRALQSPGEIDMKLVNAQQARRVLDRIVGYQGSQLL
                     WNKVRRGLSMGRVQSVAMRLICEREAEREAFRTEEYWSITALLAGTNPPAFEAKLHSI
                     NGEEASIENAEQAGRIVGEIQGKTFAVQSIERREKKRNPVAPFITSRLQQEASRKLHF
                     SPKKTMTLAQQLYEGIEIGAEGATGLITYMRTDSPRISNEAMADAREVIQSRFGADYL
                     PATANVYKTSKAAQEAHEAIRPTSAGRDPESIRQYLDHDQYNLYKLIWNRFIASQMVP
                     AILDVTRIDSTPVGTKENYLFRSTGTVVKFPGHTIVYMEGVDKEALSQKPKADQEADD
                     DERQLPALSEGERLRLVEQDGQTVPGMLSKQHFTQPPPRYNEALLIKELEEKGIGRPS
                     TYAAIISTIQDRKYVEKTEGRLVPTETGKTVNDFLLKGFPELINVDFTSQLEEQLDEV
                     EEGNKQWVAAVRDFYMPFTREMERAKTIPGPKDTVEPPTDIPCEKCGRMMEIKWGRNG
                     KFLACPAYKDDPPCKNTQ"
BASE COUNT          417 a          662 c          564 g          557 t
ORIGIN      
        1 ttgcgtgttc ttgcagggcg gatcgtcctt gtatgccggg caggcgagaa acttaccatt
       61 tcggccccac ttgatctcca tcatccgccc gcacttctcg cacggaatgt cagtcggcgg
      121 ctccaccgtg tctttgggcc ccggaatggt cttcgcccgc tccatttccc gcgtgaacgg
      181 catgtaaaaa tcacgcaccg ccgcgaccca ttgtttattt ccctcttcca cttcatccaa
      241 ctgttcttcc agctgagacg tgaagtcgac gttgatgagt tcggggaagc ccttcagcag
      301 aaaatcgtta acggtttttc cggtctctgt ggggaccaag cgtccttcgg tcttttccac
      361 gtacttgcga tcctggatcg tggagataat ggcggcgtag gtcgacggtc gtccgatgcc
      421 tttttcctcc agctccttga tcaagagcgc ttcattatac cggggcggcg gctgcgtgaa
      481 atgttgcttt gacaacatgc cggggaccgt ctgcccatcc tgctcgacca gtcgcagccg
      541 ttccccctcg gacagagcag ggagctgacg ttcatcgtcg tcggcttcct gatcggcctt
      601 cggcttctgt gacagcgctt ccttgtcaac cccctccatg tagacaattg tgtgccccgg
      661 gaacttcacc acagttccag tggaacggaa gagatagttt tctttggtcc cgacaggtgt
      721 cgaatcgatg cgggtgacgt ccaaaatggc cggcaccatc tgcgacgcga tgaatcgatt
      781 ccagatcaac ttgtagagat tgtactggtc atgatccaag tactgtctga ttgattcagg
      841 atcacgtccc gctgaggtcg gcctgattgc ttcatgcgct tcctgcgccg ccttcgaggt
      901 cttgtagaca ttcgccgttg cagggagata gtccgcccca aatcgagact ggatcacctc
      961 gcgcgcgtcg gccatcgcct cgttggaaat gcgcggcgag tcggttctca tgtaggtgat
     1021 caggccggtc gcgccctcgg cgccgatttc gatcccttca tacaactgct gcgccagggt
     1081 catcgttttc ttcggcgaga agtgcagctt ccgcgaagct tcctgctgca gccgactcgt
     1141 aatgaacggc gcgacgggat tccgtttctt ttctcgacgc tcgatcgatt gtacggcgaa
     1201 tgtctttcct tgaatctcgc cgacgatgcg accagcttgc tcggcgtttt caatcgaggc
     1261 ttcctcgcca ttaatactgt gaagcttcgc ctcgaatgcc ggtgggttgg ttcccgccaa
     1321 cagcgcggtg atcgaccaat actcttcggt tcggaacgct tcccgctcgg cttcacgctc
     1381 gcaaatcagc cgcatagcca ccgactgcac gcgccccatg ctgagcccgc gccggacctt
     1441 attccagagc aactgactgc cttgataccc cacgatgcga tccagcacgc ggcgggcctg
     1501 ctgggcattt accagtttca tatcgatctc gcccggcgac tgaagcgcgc gcttaatggc
     1561 tgattccgtg atttcgttga agaggacccg gaagatcttc ccgtctttct ttttcttctt
     1621 cgattttccg agcagctctt gctcaatgtg ccacgcaatc gcctcccctt cacgatcagg
     1681 gtccggcgcg agaaagatct tgtcggcttc ttcggccttc ttcttgatct cggccaacac
     1741 cttggacttg cccttgatag tgacgtactg cggttcgaaa tcgttctcga gatccacgcc
     1801 caatttactg gtcggaagat ccttgatatg ccccaccgac gccatgacgg tatagccgcg
     1861 gcccagatat ttcgtaatcg tcctcgcctt cgtcggcgac tcaacaatga tcaacgattt
     1921 tcccatgaca actccgtcta ctgcccaaga attgtatcat ccgtatcaaa tatcggagat
     1981 tcgtgcaact agaatggcag tcggcaacca tcagcaagat gtgaggaacc gtcgatgacg
     2041 ataggaaagt acaccggcga caaggcttat tgactatgtt cgtcacatat cattcgtcac
     2101 acgcgaagac gttcttcacg ccgatgagcc atcaattctc ccctcgatgg acgctcggcg
     2161 ggatgccttg ctgatgctag aggcgaatgt attgttgtcc
//
LOCUS       DASTWE010000929         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1608760, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000929 DASTWE010000000
VERSION     DASTWE010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608760"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VFT30_09680"
     CDS             complement(<1..1004)
                     /locus_tag="VFT30_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEU5092946.1"
                     /translation="MPQHELILLKNMMQPGYTGSLADYEKTGGYQALRNTLGKIAPPD
                     VTAMVRKSGLRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLMERD
                     PHQVLEGIVLACYAIGAETAYIYIRGEMVLGSKILEHAIGEARVAGYIGKNILGTGIN
                     ADVWVHRGAGAYICGEETALLESLEGKRGLPRIKPPFPATHGLYNKPTVVNNVETLAN
                     LPHIINRGPEWFAAIGSPPKSTGTRVFCVSGHVKRPGNYEVPMGMTVRELVYELAGGM
                     RSDKPMKAFIPGGASAPFLTPAHLDVKLDFEHVAAAGSMLGSGGVTVMEEGTSMVWAA
                     L"
     gene            complement(1026..1571)
                     /locus_tag="VFT30_09685"
     CDS             complement(1026..1571)
                     /locus_tag="VFT30_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5092947.1"
                     /translation="MILDPKSMTFSEKYKDEIADILSRYPVKRSALIPLLYVAQRDQG
                     YVTESAMQEIAHLLRLTPPQVYETITFYTMFNLKPMGKFHIQVCKSLMCALVGSDTVI
                     EWIKAKLGIAPGESTADGLFSLSAVECLAACGTGPMMQINDDYYEQLTENKLDRILAD
                     LRSTGTSPLKSGPFMWPESVN"
     gene            complement(1662..3014)
                     /gene="nuoD"
                     /locus_tag="VFT30_09690"
     CDS             complement(1662..3014)
                     /gene="nuoD"
                     /locus_tag="VFT30_09690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU5092948.1"
                     /translation="MPEDYAEGYPLRKDFPTEGKGWRSEFDFIPRLDEPPVEHIEGEV
                     PEAEKKLFLSEPTGSSSNRREELLLNMGPQHPSTHGVVRVVLELDGERIVKATPDLGY
                     LHRGVEKLAEGLAYMQIIPHTDRLDYVCAMANNYAYVRAVEKLLGITVPERAEYIRTI
                     VAEMQRILGHLFWLGAQALDIGAMTIFFWTFRERETLLDMFERLCGARLTLNYYRIGG
                     VDSDFTPELVARLKAFLEAFPQKVNEYDSLLAGNRIWLGRTKHVAVISGEDAINFGMT
                     GPALRGSGVSYDVRKLEPYGVYHKVDWEVPVGKNGDTYDRYWIRVEEMRQSARIISQC
                     LDQMPPGPIIADMPQYIPPPKPQVMRDMESLIHHFIIFTQGFKPPKGETYCATEAPKG
                     ELGFFIISDGSPRPYRLKIRSPSFIHMGAFDHMARGYLISDIITIFGTYDVVMGECDR
                     "
BASE COUNT          666 a          883 c          796 g          683 t
ORIGIN      
        1 agcgccgccc agaccatgct ggtgccttcc tccatcaccg tcacgccacc ggaccccaac
       61 atcgatcccg ccgctgcaac gtgttcaaaa tccagtttca cgtcgagatg ggccggcgtc
      121 agaaaaggcg ccgacgcccc accgggaata aaggctttca tcggcttgtc ggatcgcatg
      181 ccgccggcaa gctcgtacac caattctcgg accgtcattc ccatcggaac ttcataattg
      241 cccggtcgtt tcacatgccc gctgacacag aagactctgg taccggtgct cttcggcggc
      301 gaaccgatcg ccgcaaacca ttcgggacct cggttgatga tatgggggag attcgccaac
      361 gtctcgacgt tattgaccac ggtcggctta ttgtagagcc catgcgtggc tggaaacggc
      421 ggcttgatgc gcgggagccc ccgtttgcct tcgagcgatt ccaaaagggc tgtttcctcg
      481 ccgcagatat aagctcccgc tccacgatgc acccatacgt ctgcattgat accggtgccg
      541 agaatgttct ttccgatata ccccgcgact ctcgcttcgc cgatggcgtg ttccaagatc
      601 tttgagccca agaccatttc cccgcggata taaatgtagg cggtttctgc cccgatggcg
      661 tagcaggcca acacgatgcc ttccaatact tgatgggggt cccgctccat gagttgacga
      721 tccttaaacg taccgggctc gctctcatcg gcgttgcagc acagataacg aggcccttga
      781 tagtctttcg gcagaaatcc ccatttcaca ccggtcggga aaccggcgcc gcctcggcct
      841 cgtaacccgg acttacggac catcgccgtc acatccggtg gagcgatctt cccgagcgtg
      901 ttgcgcaaag cttgataccc tccggtcttt tcatagtccg ccagcgaccc ggtatagccg
      961 ggttgcatca tgttcttcag aagaatgagt tcgtgttggg gcatctcaat ccaacttccc
     1021 cttacttaat tcactgattc cggccacata aatggcccgc tcttcagcgg actggttcct
     1081 gtcgatcgca gatcggccaa aattcggtcc agtttgttct cggtcagttg ctcatagtag
     1141 tcatcgttga tctgcatcat gggaccggtc ccgcacgcgg ccaaacattc caccgcactg
     1201 aggctgaaca gtccgtcggc cgtcgattcg cccggggcga tgcccagctt cgctttgatc
     1261 cattcgatga ccgtatcaga gccgacgaga gcgcacatga gagacttaca cacttgaata
     1321 tggaatttgc ccatcggctt caggttaaac atcgtgtaaa aggtgatcgt ctcatagact
     1381 tgcggagggg tcagcctgag aagatgcgca atctcctgca tcgccgactc ggtcacatag
     1441 ccttgatcac gctgggcgac atagaggagt gggatcaaag ccgaacgctt gacagggtag
     1501 cgcgacaaga tgtcggcgat ctcgtcttta tatttttctg aaaaagtcat gctttttgga
     1561 tcaagaatca ttggccaaca gtcaatggat gatgcgccat agttaacaag caggcaggaa
     1621 tattcccgcc gttgaacgaa gacagtgacc agtcgctcta ttcatcgatc acattccccc
     1681 atgaccacat cgtaggtccc gaaaatcgtg atgatgtcgg aaatcaagta gccgcgtgcc
     1741 atatggtcga acgcgcccat gtgaatgaag gagggtgagc gaattttcaa ccgataaggg
     1801 cgtggagatc catcactaat gataaaaaat cccaattcac ccttgggcgc ttcggtcgcg
     1861 caataggttt cgcccttcgg tggtttgaac ccttgggtga agatgatgaa atggtgaatc
     1921 aagctttcca tgtcgcgcat cacttgcggc ttgggcggcg gaatgtattg cggcatatcg
     1981 gcgatgatcg gcccgggcgg catctgatcg agacattgcg agatgatccg agcactctga
     2041 cgcatttctt caacacgaat ccaatagcgg tcgtaggtgt cgccgttttt tccgaccggt
     2101 acttcccaat cgactttatg gtagacgccg tagggttcga gtttgcgtac atcataggac
     2161 acaccggatc cccgcaaggc agggccggtc atgccgaaat tgatcgcatc ttccccggag
     2221 atgacagcga catgttttgt ccgacccaac caaatccggt tgcccgcgag cagcgaatca
     2281 tactcgttca ctttttgggg gaatgcctcc aggaatgcct tgaggcgggc aactaattca
     2341 ggcgtgaagt cgctatccac gccaccgatc cgataataat tcaaggtcaa tctggctcca
     2401 cacagccgtt caaacatatc gagcaaggtt tctcgctcgc ggaacgtcca gaagaagatc
     2461 gtcatggcac cgatatccag cgcctgtgca ccaagccaga acagatgtcc cagaatgcgc
     2521 tgcatctccg cgacaatggt gcggatatac tcggctcgtt caggcacggt gataccgagg
     2581 agcttctcca cggcgcgcac atacgcataa ttgttggcca tcgcgcagac atagtcgagc
     2641 cggtcggtat ggggaatgat ctgcatgtag gcgagaccct cagccaattt ctcgacgccc
     2701 cgatggaggt atcccagatc cggagtcgcc ttgacgatcc gttctccgtc cagctcaaga
     2761 acgacccgca ccacgccgtg cgtgctcgga tgttgtggac ccatgttgag caacagttct
     2821 tcccgccggt tcgatgagga cccggtcggt tcggaaagaa acaacttctt ctctgcctcg
     2881 ggaacctcgc cctcgatgtg ctcgaccggc ggttcatcga ggcgcgggat aaaatcgaac
     2941 tcgctgcgcc agcccttccc ttccgtcgga aaatcctttc tgagcggata cccttccgcg
     3001 tagtcttccg gcatgaggat gcggcgta
//
LOCUS       DASTWE010000930         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1156300, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000930 DASTWE010000000
VERSION     DASTWE010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156300"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFT30_09695"
     CDS             <1..267
                     /locus_tag="VFT30_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5092949.1"
                     /translation="TLRIGAPSDNAFVRFEVIDTGVGIKPENLPGLFAQGFTTRKAGH
                     GLGLHSAAIVAKNLGGTLQALSMGEGQGATFTLDLPLVVVEAAA"
     gene            264..2429
                     /locus_tag="VFT30_09700"
     CDS             264..2429
                     /locus_tag="VFT30_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5092950.1"
                     /translation="MINEGTTDNRRILIIDDNADIHEDFRKILAPPKDSDTLNQARAA
                     LFDEASPQGRSEQFVVDFADQGESGFRMVKEAVSSGTPYALAFVDMRMPPGWDGMETV
                     RHLWEADGDIEVVICTAFADYSWEEIVSALSQRDKFLILRKPFDAIEVSQLASCLTQK
                     WNLAKHAHLKLNDLECMVEKRTVELTKARDQALAAARAKSEFLATMSHEIRTPMNGVI
                     GMTGLLLETDLTSEQREYTETVQKSGDHLLALINDILDVSKMEAGKLELETIDFDLRT
                     TVESVTEILAEPAQRKGLELVSLIHASTPIAMRGDPARLRQILMNLVGNAVKFTAQGR
                     VVLQVRTTEERGDQVILLFEVTDTGIGIPSEKQGMVFDAFSQADGSTTRRFGGTGLGL
                     AIVKRLVTLMKGTIGVESKEGQGTRFWFIVPLTPQPSPPAVHLPVADLRDRRVCIVDD
                     DPAGRMVLEQYTKHWSMQPSSAANASSALALLREAVQQKRPFDVVLVDIGLPVLTGVE
                     LAGMIKQDSLITDTPIIILTSYGQRGEAKLAAEVGAAGYLTKPLRYAQLHACLRLLFS
                     QSVQKNVASSGQLALVTRHTLAEAKARQAGRILMADDSDISQVLVVRLLERAGYRVDV
                     VGTGVEALAALAQNTYDLLLLDCFMPNMDGYETARRIRAAEPPGRRMPIVALTANAFP
                     EDRQKCFASGMDGFLVKPIIRDDLLTAVAQWLPGRSMNG"
     gene            2534..>3085
                     /locus_tag="VFT30_09705"
     CDS             2534..>3085
                     /locus_tag="VFT30_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092951.1"
                     /translation="METTDQNRRILVIDDNVNIREDFRKILTPPNDSDSLLQARAALF
                     GEVPTLPPQVRYELDFANQGREGFGLAQSAHQKGNPYAMAFVDMRMPPGWDGLETIEH
                     LWYVDPDIQIVICTAYSDHPWEDVSRRIGDTDKLLILMKPFNSIEVVQLARSLTKKWN
                     LARLVKLQVESLAVSVIQRTAELR"
BASE COUNT          702 a          838 c          902 g          643 t
ORIGIN      
        1 accttacgca ttggtgcacc ttccgacaac gcattcgtgc gatttgaggt gatcgatacc
       61 ggggtcggga tcaaaccaga aaaccttccg ggattgttcg cacaaggctt tacgaccaga
      121 aaggctggtc atgggcttgg cctccatagc gcagctattg ttgccaagaa tctcggcgga
      181 acactgcaag cgctaagcat gggtgaaggg caaggcgcga cttttacgct cgatcttcct
      241 ttggtcgtgg tggaggctgc agcatgataa acgagggcac cactgacaat cgacgaattc
      301 tgatcatcga cgacaatgct gatattcatg aggacttccg caaaatctta gcgccgccga
      361 aggattcgga cacgctcaat caggctcggg ctgctctgtt cgacgaagcc tcgccccaag
      421 ggcgaagcga acaatttgtg gtggacttcg cggatcaggg tgagagcggc ttccgcatgg
      481 tgaaagaggc tgtgagttcc ggcacgccgt acgcgctggc tttcgtcgac atgcgcatgc
      541 ccccaggatg ggacggaatg gaaacagtcc gacatttgtg ggaggccgac ggagacattg
      601 aggtcgtgat ttgtaccgcc tttgcggact attcgtggga ggagatcgtc tccgcattga
      661 gccagcggga caagtttctc atcctgcgta aacccttcga tgcgatcgaa gtgtcccaac
      721 tggcctcatg cctgacacag aaatggaact tggcgaaaca tgctcacctc aagttgaatg
      781 atctcgaatg catggtcgaa aagcgcacgg tggagctcac gaaagcgcgt gatcaggcct
      841 tggcggccgc cagggccaag tctgaatttc tggccaccat gagccatgaa atccgcacgc
      901 cgatgaacgg cgtgatcggt atgacgggcc tcctgctcga aacagacctg acctccgaac
      961 agcgcgaata taccgagacc gttcagaaat cgggggacca tcttctcgcg ctcatcaatg
     1021 acatcctcga tgtctccaag atggaagcag gcaagctgga gctcgagacc atcgacttcg
     1081 atctgcgcac caccgtggag tcagtcaccg aaattctcgc tgaaccggcg cagcgaaagg
     1141 gcttggagct cgtctccttg attcacgcct ccacacctat tgccatgcgt ggagacccgg
     1201 cgcggcttcg ccagatcctc atgaacctcg tcggaaatgc cgtgaaattc accgcgcagg
     1261 gacgcgtggt gcttcaagtt agaaccaccg aggaacgcgg agaccaggtg attctgcttt
     1321 ttgaagtgac ggataccggc atcggaatcc cttccgagaa gcaaggcatg gtcttcgatg
     1381 cattttcgca ggccgacggc tcaactaccc gacgattcgg cggcacgggt ctcggcctgg
     1441 cgatcgtgaa gcggctggtc acgcttatga agggaactat cggcgtcgaa agcaaggagg
     1501 gccagggaac ccggttctgg ttcatcgtac cgttgactcc tcagccatca ccgccggccg
     1561 tccatctgcc tgtcgctgac ctgcgggatc gccgagtgtg cattgtggat gatgatccgg
     1621 ccggccgaat ggtgcttgaa caatacacga aacactggtc gatgcaaccg tccagcgccg
     1681 cgaatgcctc gagtgcgttg gctcttcttc gggaggcggt tcaacagaag cggccgtttg
     1741 atgtggttct tgtcgatata ggattgccgg tccttaccgg tgtcgagctt gccggcatga
     1801 tcaagcagga ttcgttgatc acggatactc cgatcattat tttgacatcc tatggacagc
     1861 gaggagaagc caagctcgcg gcggaggtcg gcgctgccgg gtatctgacc aagcccctac
     1921 ggtatgcgca gctccatgca tgtcttcggc tcctgttcag tcaatctgtg caaaagaacg
     1981 tcgcgagctc aggacaactc gctctcgtca cgcgtcatac actggcagag gcaaaagcaa
     2041 ggcaggccgg gcgcattctg atggcggatg acagcgacat cagccaagtg ctggtcgtgc
     2101 gtttgctcga acgggcggga tatcgagtcg acgtggtagg aaccggcgtg gaggcccttg
     2161 cggcgctcgc ccagaatacc tacgatcttc ttcttctcga ctgtttcatg ccgaacatgg
     2221 atggatacga aaccgcgcgg cggatacgtg ccgcagaacc gcctggacga cggatgccga
     2281 tcgtcgcctt gaccgccaat gcttttccgg aagatcggca gaagtgtttc gcgtcgggaa
     2341 tggacggctt cctggtgaag ccgatcattc gagatgatct cctcacggcc gtcgcccagt
     2401 ggctgcctgg gcgctcgatg aacgggtaaa catcctcagg ttactggccc gccgatccga
     2461 tagtagtacg agaaacccag gcagcatcca cgccaagaaa agggcggtac gggcataaga
     2521 aaaaggaaga ggtatggaga caacggatca aaaccgtcga atcttggtga tcgatgacaa
     2581 tgtcaacatc cgagaagatt tccgcaaaat cttgacgcct ccgaacgatt cggattcact
     2641 ccttcaagct cgcgccgcct tgtttggaga agttccgact cttccgccac aagtgcgcta
     2701 cgaattggac ttcgcgaatc agggacgaga gggattcggt cttgcccaga gtgctcatca
     2761 aaagggcaat ccctatgcga tggcgttcgt ggatatgcgc atgccgccgg gatgggatgg
     2821 tctcgaaacc atcgaacatc tttggtatgt cgatcccgat atccaaatcg tgatttgcac
     2881 ggcctattcc gaccatccat gggaagatgt gagtcgccgt atcggagaca ccgacaagtt
     2941 gctcatcctg atgaaaccgt tcaacagtat tgaagtggtt caattggcca ggtcgctgac
     3001 gaaaaaatgg aacctggccc gcttggtcaa actgcaggtt gagagccttg cggtcagtgt
     3061 gatccagcgg acagcggaat tacgg
//
LOCUS       DASTWE010000931         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1415155, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000931 DASTWE010000000
VERSION     DASTWE010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415155"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFT30_09710"
     CDS             <1..843
                     /locus_tag="VFT30_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU5092952.1"
                     /translation="RLREQFVGDQIDWAQIRIIQQAMSPLQQDSAERTLLERLDECFR
                     IFLTCSPDDRRRIQRVMTTLTQGMEMDLSTFPGASVADLTALKTLDDLDRYTYHVAGC
                     VGEFWTSLMCAHRKALRNWDTLRMSEVGVRFGKGLQLTNIVKDIAHDLQKGRCYIPEM
                     MLTEAGLKPYDLLDQNNLPRFRPVLGRLVRLAVEHLDQGWLYTMSIPRREARLRLACM
                     WPILSAGESLKLVMNSTDLLNPSVKVKIPRSKVYQIMAMTTGTVGCGYAGTAYWGHLR
                     KQIV"
BASE COUNT          267 a          307 c          319 g          285 t
ORIGIN      
        1 cgcctcagag agcagtttgt cggtgatcag atcgattggg cacagatccg tatcattcaa
       61 caagccatga gtccgcttca acaagattcg gctgagcgaa ctctactcga acggttggac
      121 gagtgtttcc gaatttttct cacctgttcg cccgacgacc gacgtcgaat tcaacgagtg
      181 atgacgaccc tgactcaagg aatggaaatg gatctgagca ccttccctgg agcttccgta
      241 gcggatctca ctgcgctcaa gacgctcgat gacttagatc gctataccta tcatgtggca
      301 ggatgtgttg gtgagttctg gacttccttg atgtgtgccc atcggaaagc gttgaggaac
      361 tgggacaccc tgcggatgtc ggaagtcggg gtacgatttg gaaagggact acaactgacg
      421 aacatcgtca aagacattgc ccatgatctg caaaaggggc gttgttacat tcccgagatg
      481 atgctcaccg aagctgggct caagccatat gatctattag atcagaataa tcttcctcgt
      541 ttcaggccag tgctcggcag actcgttcgc ttagccgtcg agcatcttga tcagggctgg
      601 ctctatacga tgtcgattcc acgtcgtgaa gccaggttgc gacttgcgtg catgtggccg
      661 attctttccg ccggagaatc tctcaagctc gtcatgaact ccaccgatct gttgaatcct
      721 tcggtcaaag tcaaaatccc tcgcagcaag gtctatcaga tcatggccat gacgactggt
      781 acggttggtt gcggatatgc cgggactgca tactggggac atttgcggaa acagatcgtg
      841 taagtcgccc ggatggttgt tctctctttt cttccttcct cctacggcgg atggaacgga
      901 agagtgggga gatatgcgcc atcgcgctga acatatggca aaggtctcga aaaagtgagc
      961 gggcgacggc atgctgctac gcagcgtttt agaggagccc gcggcgatca acatgcacgg
     1021 acctttacat gatggcgcaa tcgaggccat gctccgttcc gtccgccgct cttctacttc
     1081 ttctgcggct ccagcaactt gtcgcctttc ctaaacactg cgaagattgc ctgtgaggga
     1141 caggcatcca ggcagagtcg acaactttcc agacagcg
//
LOCUS       DASTWE010000932         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_251623, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000932 DASTWE010000000
VERSION     DASTWE010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251623"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1215)
                     /locus_tag="VFT30_09715"
     CDS             complement(<1..>1215)
                     /locus_tag="VFT30_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU5092953.1"
                     /translation="PTPFPEFDLTISQADSPDPVFVGRDLTYTLTVVNSPAALGGGAC
                     PEVRFRFPSGVPIAFVSASGTNGFVASPTSNGLSFTGGCISSAGGTTGTATITIIIRA
                     LATGTLTSVGTDVVVDPDDLINENNDNNNTAATIQTTVIAAPDAKTGFDYDGDDRADV
                     SVFRPSEGVWYLNRSTAGFIGTQFGIATDKIVPADYDNDGKTDIAVFRPSEGTWYINN
                     SSNGGFTIVQFGIASDIPVPADYDGDGKADIAVFRPSEGVWYIQNSSDGSFTIYQFGS
                     NGDRPTLGDFDHDGKSDIAVFRPSTGVWYHLRSSDGTFFAEQFGISSDQPTPADYDGD
                     GQTDLAVFRASDGAWYVKNSSNSTYSSFFWGAAGDIPVAADYDGDGKANIAVFRPSGG
                     SWYVLNADGSFNS"
BASE COUNT          299 a          349 c          312 g          255 t
ORIGIN      
        1 gaattgaacg acccatcggc attgaggaca taccaggaac cacccgatgg acggaacact
       61 gcaatgttag ccttgccgtc gccatcatag tccgctgcaa ctggaatatc acctgccgct
      121 ccccagaaga atgacgagta cgtgctgttg gaactgttct tcacgtacca ggcaccgtcc
      181 gatgcacgaa agacagccag atcggtttgt ccatccccat cataatcagc aggtgtcggc
      241 tggtcagaag agataccgaa ctgttctgca aagaacgtac cgtccgaact cctcaagtga
      301 taccaaacac ccgtcgatgg cctgaagaca gcgatgtctg acttaccgtc atgatcgaaa
      361 tcgccgagcg tcggacgatc gccattcgac ccgaactgat agatcgtgaa cgagccatct
      421 gagctattct ggatatacca gactccttct gatggacgga acacagcgat gtcagccttg
      481 ccgtcaccat cataatctgc cggtaccggg atatcactcg ctataccaaa ctgtacgatc
      541 gtgaaaccgc cgtttgagct gttgttgata taccatgtgc cctcggatgg tcggaatacg
      601 gcaatatcgg tcttgccatc gttgtcatag tcagccggca ctatcttgtc cgtagcaata
      661 ccgaactgcg tgccgatgaa tcccgccgtt gaccgattga gataccaaac accctcggat
      721 ggacgaaaca ccgagacatc agcacggtca tcgccgtcgt aatcaaatcc ggtcttcgca
      781 tccggagctg cgatcactgt cgtctggatg gtcgccgcgg tgttgttatt atcgttgttc
      841 tcgttgatca gatcatccgg gtcgaccacg acgtcagtgc ccacgctggt tagggtgccg
      901 gtcgcaagcg ccctgatgat gatagtgatc gtagccgtgc cagtagtgcc gccagcggaa
      961 ctgatgcacc cgccggtaaa ggaaagcccg ttcgatgtag gcgaagcaac aaaaccattc
     1021 gtccccgacg ccgatacaaa ggctatcggg accccacttg ggaatctgaa tcgaacttcc
     1081 ggacatgctc caccacccag tgcagccggc gagttcacga cagtcaacgt ataagttaag
     1141 tcgcgcccga caaacaccgg atcaggggaa tcagcctgac tgatggtcag gtcaaattcg
     1201 gggaacggcg tcggt
//
LOCUS       DASTWE010000933         1947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1027317, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000933 DASTWE010000000
VERSION     DASTWE010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027317"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /gene="mutL"
                     /locus_tag="VFT30_09720"
     CDS             complement(<1..1081)
                     /gene="mutL"
                     /locus_tag="VFT30_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU5092954.1"
                     /translation="MLRTDGSGKICILPEEVIGRIAAGEVVERPAAVVKELLENSLDA
                     GSQSITIDVRDGGLALIRVSDDGEGMSREDVPRAFQRHATSKLRSDLDLWSIRTMGFR
                     GEALPSIAAVAHVRLMTATRLAEVGTELEVVGGTIGRIAEAPPVTGTRIEVAELFHNQ
                     PARKKFLKSTLTEFLHISRVVQQASLAWPSIHFRLTHNAEEIFNYPAAPSDEDRIAQV
                     YRRTFLDQCLRIKASFPGASVSGYIVDGVYAKPARIPQELFVNRRPVRNAAVSHAVGE
                     GYGSFLAKGSQPRFVLFLEIDPDRLDVNVHPTKREVRFSDNELIHQLVRRAVRQALSG
                     GKTDELGKTPFIESSVRRTTASIVTT"
     gene            complement(1145..>1947)
                     /gene="ybgF"
                     /locus_tag="VFT30_09725"
     CDS             complement(1145..>1947)
                     /gene="ybgF"
                     /locus_tag="VFT30_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU5092955.1"
                     /translation="GQTGSTQGGAVNNMRVRTLASGSAAWGLLASTVVLVGCAKHADF
                     LEMRNQLSTISRTHDQDHQRMDAVMRRLEAVERNKDTEPARPRFDDLSARFQKLESRL
                     AKLEEAASQASAKVDASTEPRASRTAKQTQSAEPTVTVSGITPTSAFNLAYNDYLNGK
                     YELAVAGFQRFVKDFPGTSLTPNAQYWLGESYYNLKDYGRAIQTFDYLVAEYPGNEKV
                     PAALYKLGLATAETGDLGRSRKNLKRVLEEFPSSDESKLAKNKLAEIR"
BASE COUNT          401 a          587 c          529 g          430 t
ORIGIN      
        1 gagttgtcac aatggaagcc gtggtccgtc gcacggagga ctctatgaac ggagtcttcc
       61 caagttcatc cgtctttccg ccactcaagg cttggcggac cgctcgccgt acgagttggt
      121 ggatcaactc attgtccgaa aatcttactt cccgcttggt tggatggaca ttgacatcga
      181 ggcggtccgg atcaatttcc agaaacagca cgaatcgcgg ttgactcccc ttggcaagga
      241 atgacccata cccttccccg acggcatgag agactgcggc gttgcgcaca ggacgacggt
      301 tcacaaacaa ctcttgcggt atgcgagcgg gctttgcata gactccatcc acgatgtacc
      361 cgctgacaga agcgccaggg aacgaggcct tgatgcggag acactggtcg agaaaggtgc
      421 gccgatagac ctgggcaatc cgatcttcat ccgacggcgc ggccgggtaa ttgaagatct
      481 cctcggcatt gtgcgtcaaa cggaaatgga tggacggcca ggcaagcgat gcctgctgta
      541 cgacccggct gatatgcaag aactccgtga gggtcgactt cagaaacttc ttccgggccg
      601 gctgattgtg gaacaactcc gcaacttcga tgcgtgttcc cgtcaccgga ggggcttcgg
      661 cgattctccc gattgttcct ccgaccactt ccaactcggt cccgacttcg gcaagacgag
      721 tcgccgtcat cagccgaaca tgggcgacag cggcgatgct cggcagtgct tcgccccgga
      781 aacccatcgt tcggatggac cagagatcca gatcggaccg aagcttgctt gtggcatgcc
      841 gctgaaacgc gcgcgggaca tcttcccgac tcatgccctc cccgtcgtca ctcacccgaa
      901 tcaaggcgag ccctccgtcc ctcacatcga tcgtgatgga ctgactcccc gcatcgagac
      961 tgttctcgag gagctctttc acgaccgccg ccgggcgttc gaccacctct cccgctgcaa
     1021 tgcgaccgat gacctcttcc ggaagaatac agatcttgcc actgccgtcg gttctcagca
     1081 cggcacgatg ctcctcatga gcgatgagac ggtgatgggg cggggcgtcg cacgacggcg
     1141 acgatcatcg gatttcggcc aacttgttct ttgccaactt tgattcgtcc gatgagggga
     1201 actcttcaag cacacgcttc agattctttc tcgacctgcc gagatcgccg gtctcggccg
     1261 tcgccaaacc aagcttatac agtgccgcag gaaccttttc gttccctgga tattccgcca
     1321 cgagatagtc gaacgtttgg atggcccgcc catagtcttt caggttgtag tacgattctc
     1381 ccagccaata ttgagcgtta ggggtaagag acgtgccggg aaaatctttg acgaaccgtt
     1441 ggaacccagc gaccgcaagc tcgtatttcc cgttgagata atcattatat gccagattga
     1501 aggcggacgt cggcgtaatt ccggacaccg tcaccgtcgg ttctgcagat tgcgtctgtt
     1561 tggccgtcct ggatgcacgc ggttccgtcg aagcatccac cttggcggat gcctggctgg
     1621 cagcttcttc cagctttgcc aatctgctct ctagtttttg aaagcgtgcc gacaggtcat
     1681 cgaatcgcgg ccttgcaggt tccgtatcct tgttcctctc cacggcctcc aatcggcgca
     1741 tcacggcatc catacgttga tgatcctggt catgtgttcg cgagatggtc gagagctgat
     1801 tgcgcatttc cagaaaatcg gcatgcttgg cacagccgac aagcacgacc gtcgacgcca
     1861 acaaccccca tgccgcagaa ccggatgcaa gtgtacggac acgcatattg tttaccgcac
     1921 ctccttgagt tgagccagtt tgtccga
//
LOCUS       DASTWE010000934         1072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1608991, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000934 DASTWE010000000
VERSION     DASTWE010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608991"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFT30_09730"
     CDS             complement(<1..1014)
                     /locus_tag="VFT30_09730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5092956.1"
                     /translation="MPYMVEKWDEKPPRWDLLPPGYMFRDALTATRGCNYRCTFCSIH
                     LALGGGQYGFRKKPPADVVKLVEKMNGPMVMFWDDDLLSDPAYTRELCAAMKPLGKKW
                     MSQMSATYVAHHPELLKTLKEAGCSAMFMGIESINQDSLKSVDKQNQAKLYEEMIKRI
                     HDHGIDIHAGFICGLDHEDVFDFERTAEWATKMGLAGALWRIMTPYPGTKQFAELKAA
                     GRILTENWTAYTGEHVVYKPAKMTVEQLYWGHKWAKGQFYSFKSIGQRAIQRASQNGF
                     GELLNITGCGLGYRSMFHLAADSVPVNVYRDMNHLPPQEEPIAYRFPYPPKKRFSFIT
                     DRMD"
BASE COUNT          226 a          304 c          272 g          270 t
ORIGIN      
        1 gtccattcga tccgtgataa agctgaatcg tttcttcggg ggatagggga accgataggc
       61 aatcggctcc tcctggggcg gcaagtggtt catgtcgcga tagacattca ccggaacgga
      121 atccgctgcc aagtggaaca tcgaccggta tcccaatcca cagccggtga tgttaagcaa
      181 ctcaccgaac ccgttttgag aggcccgctg gatggccctc tgtccaattg acttaaacga
      241 atagaactgt cccttggccc acttatgtcc ccagtacaat tgctcgacag tcattttggc
      301 cggcttgtac acgacatgct ctccggtata agcggtccaa ttttccgtga ggatccgccc
      361 ggctgctttg agttccgcaa actgcttggt gccgggatag ggggtcataa tgcgccagag
      421 cgctcctgcc aaccccattt tcgtcgccca ttcggcagtg cgctcgaagt caaagacatc
      481 ttcatggtcc aagccgcaaa taaatcctgc atggatatcg attccgtggt catgaatgcg
      541 cttgatcatc tcctcatata gcttcgcctg attttgttta tcgacggatt taagtgaatc
      601 ttgattgatg gattcgattc ccatgaacat cgccgaacag ccggcttctt tcagcgtctt
      661 gagtaattcc ggatgatggg cgacgtacgt ggcgctcatc tggctcatcc acttcttccc
      721 gagcggtttc atggccgcgc acagctcacg cgtgtaggcc ggatccgaca ataggtcatc
      781 gtcccaaaac atcaccatcg gcccgttcat tttctcgacg agcttcacca catcggccgg
      841 tggttttttt cggaatccat actggccgcc tcctaaggcc agatgaattg agcagaatgt
      901 gcagcggtag ttgcaccctc tggtcgctgt caatgcgtcc cgaaacatat aaccgggcgg
      961 taacagatcc cagcgtggag gcttctcgtc ccatttctcg accatgtacg gcatgcggta
     1021 ctgcggttgc atctggcctt ttttgaaatc ctcgagcagc cgcgggacag tc
//
LOCUS       DASTWE010000935         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_251659, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000935 DASTWE010000000
VERSION     DASTWE010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251659"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..>1576
                     /locus_tag="VFT30_09735"
     CDS             197..>1576
                     /locus_tag="VFT30_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5092957.1"
                     /translation="MTTSSTLILPTRAHAIAHDLRALLGTEKVKDDAPTITAYAVDAS
                     IYRIPPQAVVLVESEEDIAATVAYAVTRGIPLTPRAAGTNLTGSAIGSGIILDVSRLN
                     RILEVNREERWARVQPGMVLAELNKQLSLQGLLFGPDPSSGDMCKLGGMVANNSSGPH
                     TLRYGSVKDNVQSLRLCLTSSSWIEARAYALDDPSLERLLATVPALRDVLMMTQAHTD
                     LIAAKRPTVSKNSCGYNLFGLADGLARGSFDLPKLFVGSEGTLGVVSEARLTLVDKPK
                     ATLTALIHFRSLEEVGEAVPQLLTLQPSALEVMDANTLNLIGRGKHGIPADAAATLLI
                     ELDADSTETDLHERADAMAAVCRPYKLASELTLAFDAERREQLWKARKALYPTLYRFD
                     PKKKPINFVDDVVVPAGRISELIRYLENFFEGQRVPVAIFGHIGNGNAHIVPLLDVND
                     SGDFEKMVSA"
BASE COUNT          333 a          442 c          466 g          335 t
ORIGIN      
        1 ccgtcggtgg ccgatctgtc ggctcggcca gcatccgata ctgtccggag attgcgtcca
       61 gcaccggccg aggcaacagt cgggtgatat ataaagtcgg acggtccatg ggctttccat
      121 tgtaggagaa ttcgtaggca accgacaacg acgctggtgc ttcgaagggt ccacgagcta
      181 agatgcttga ttacctatga cgacttcatc gacattgatc ttgccgacga gagctcacgc
      241 aattgctcat gaccttcgcg ctctcctggg aacagagaaa gtcaaagacg acgcgcccac
      301 gatcacggcc tacgccgtcg atgccagcat ctatcgcatt cctccacagg cggtcgtcct
      361 tgttgaatca gaagaggaca tcgctgcaac tgtcgcctat gcggtgaccc gcggcattcc
      421 tcttacacct cgggcagccg ggacgaacct gaccggttct gccatcggct caggcatcat
      481 tctcgacgta tcgcgtctga atcgaatcct tgaagtgaat cgggaagaga gatgggctcg
      541 ggtccagccg ggaatggtgc tggccgaatt gaacaaacaa ctgagcttgc aggggctgtt
      601 gtttgggccg gatccatcga gcggggacat gtgcaaattg ggcggcatgg tcgccaataa
      661 ttcctcggga ccgcacactc ttcgctacgg gtcggtaaaa gacaatgtcc agagtctccg
      721 actgtgcctc acttcgtcgt cctggatcga ggcgcgagcc tatgccctcg atgatccgtc
      781 gctggaacgc ctcttggcga cggtgccggc cttgcgtgat gtcctgatga tgacacaggc
      841 ccatacagac ctcattgccg cgaagcggcc tacggtcagc aagaatagtt gcgggtacaa
      901 tctctttgga ctggccgatg ggctcgcacg gggctctttc gacctcccga agctattcgt
      961 cggcagcgaa gggacgctgg gtgtggtgag tgaagccaga ctcacgctgg tcgacaagcc
     1021 caaagccacg ctgaccgcgc ttattcattt ccggagtctt gaagaggtcg gcgaggccgt
     1081 acctcagctc ctgacgctcc agccgagcgc tttggaggtc atggatgcca atacactcaa
     1141 cttgatcggt cgagggaagc atggcattcc ggctgatgcg gcggcgacgt tgctgatcga
     1201 gttggatgct gattccaccg agaccgatct ccatgaacgg gctgatgcca tggccgccgt
     1261 ctgtcggcct tataagttgg cgtcggaact tacactcgcg ttcgacgccg aacgtcggga
     1321 gcaactctgg aaagcgagga aggcactcta tccgactctc tatcgattcg acccgaagaa
     1381 gaaacccatc aattttgtgg atgatgtcgt cgtgccggcc ggacgcatca gcgagctgat
     1441 ccggtatttg gaaaacttct tcgaagggca gcgagtgccg gttgcgatct tcggccatat
     1501 tggaaatggg aacgcgcaca tcgttcccct gctggacgtg aacgattccg gcgatttcga
     1561 aaagatggta agcgcc
//
LOCUS       DASTWE010000936         1218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_154554, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000936 DASTWE010000000
VERSION     DASTWE010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154554"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..305)
                     /gene="secE"
                     /locus_tag="VFT30_09740"
     CDS             complement(111..305)
                     /gene="secE"
                     /locus_tag="VFT30_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU5092958.1"
                     /translation="MFQRLIASVREFIEGVRGELRKVSFPTKTETVGATTVVIVFCIL
                     MSLYLSMMDSVLGWLMRKVI"
     gene            complement(397..471)
                     /locus_tag="VFT30_09745"
     tRNA            complement(397..471)
                     /locus_tag="VFT30_09745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Trp,seq:cca)
     gene            complement(530..679)
                     /gene="rpmG"
                     /locus_tag="VFT30_09750"
     CDS             complement(530..679)
                     /gene="rpmG"
                     /locus_tag="VFT30_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5092959.1"
                     /translation="MREIIDLACTVCKQRNYTTRKNKKNDPDRLERNKFCKFCRKHIA
                     HKEVK"
     gene            complement(768..>1218)
                     /locus_tag="VFT30_09755"
     CDS             complement(768..>1218)
                     /locus_tag="VFT30_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEU5092960.1"
                     /translation="VGLRPTQTTIVTGVEMFRKVLDEGQAGDNIGVLLRGTKKEDVER
                     GMVLAKPKSITPHTKFKAEIYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGIVTLTPG
                     VEMVMPGDNVTVTGELISPIAMDQGLRFAVREGGKTVGSGVVTEILA"
BASE COUNT          266 a          403 c          284 g          265 t
ORIGIN      
        1 cgcataggta tggatgacgt accagttctt attgctcatc atctcctctc agctgaacat
       61 cagcatcgta ttgtccggca atgccggacc tgtccagccg cgccacgcaa ctagataacc
      121 ttgcgcatca gccagcccag cacggaatcc atcatcgata agtacaagga catgagaatg
      181 cagaacacaa tcacgaccgt ggtggcgccg accgtttccg tttttgtcgg aaaggacact
      241 ttccgcaatt cgccgcgcac accttcgata aattcacgaa cagaggcgat caatcgctga
      301 aacacggtta caatcctctc gtagacttcc cggctgtcag tttgatccaa accgaccgat
      361 cacatcacac acctacccat acaaaagcgc tcggcttggc aggggcacta ggatttgaac
      421 ctagactctc ggttttggag accgatgtgc tgccgttgac accatgcccc tgcaatcgga
      481 acatgagcgc tgccgagcca gggacaaggt cccgcccgac acaaaagcct tacttcactt
      541 ccttatgagc aatgtgcttg cggcaaaact tgcagaactt gtttcgctcc aaccggtccg
      601 gatcgttctt cttgttcttg cgggtcgtat agttacgctg cttacaaacc gtgcaggcca
      661 agtcaataat ttctcgcatg tcgtataccc gtgaagcgtg taacgtgaag tcggagagta
      721 ggatcgcctc cgcgctcgaa acgcctgacg cctcacgcct ccctctacta agctagaatt
      781 tccgtaacga cgcccgagcc gacggtcttg ccgccttcgc gcacggcgaa ccgcaacccc
      841 tgatccatcg caatcgggct gatcaactcg cccgtcaccg tgacgttatc gcccggcatc
      901 accatctcca cccccggcgt caacgtcacg atccccgtca cgtccgtcgt ccggaagtag
      961 aactgcggcc ggtagccgtt gaagaacggt gtatgccgtc cgccctcttc cttcgtcaac
     1021 acatagatct ccgccttgaa cttcgtgtgc ggcgtgatac tcttcggctt ggccaacacc
     1081 atgccacgct ccacatcctc tttcttcgtg ccgcgcaaca agacgccgat gttgtcgccc
     1141 gcctggccct cgtccaacac cttgcggaac atttcgaccc cggtcacgat ggtcgtctgc
     1201 gtcggccgca agcccacg
//
LOCUS       DASTWE010000937         1291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_348511, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000937 DASTWE010000000
VERSION     DASTWE010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348511"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFT30_09760"
     CDS             <1..213
                     /locus_tag="VFT30_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092961.1"
                     /translation="GYRIAASQHNPILVTLDHHKHYATLWSLKFPRYGDVDAGGEWVE
                     VTYIYMQLCPIFDYHVKPALSSGIEY"
     gene            complement(248..>1291)
                     /locus_tag="VFT30_09765"
     CDS             complement(248..>1291)
                     /locus_tag="VFT30_09765"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEU5092962.1"
                     /translation="PPTPFQFATAGYRYNFPRDHGSHPTYRTEWWYYTGHLHSKSGRA
                     FGFELTFFRRGVPPDEIKTLPSKWSVKDLYLAHFAVTDITGKRFHFSEKFSREGLGKA
                     GADESRLRVWIDDWRAEVLTEPSASHTLVAHDKTHALTLTLLPAKPLVTHGAAGISRK
                     GKEVGQASHYYSFTRLATSGKLTIDGESFDVTGTSWMDHEFGSAELGTDQVGWDWFSI
                     QLEDDTELMLYRMRRKDGSSDLASSGTAVSPDGRPRHLEVTDFQIESTGTWTSAESKA
                     TYPNQWRLKFPSLDLVLDVTPLLADQELRTSRSTKVSYWEGAVAVTGTKQGRPVKGQG
                     YVELTGYAERLKM"
BASE COUNT          279 a          367 c          351 g          294 t
ORIGIN      
        1 ggctacagga ttgcagcgtc acagcacaac cccatcttag tcaccctaga ccatcataag
       61 cactacgcga cactctggag tcttaagttt ccgaggtatg gagatgtcga cgccggaggc
      121 gaatgggttg aggtgactta tatctacatg cagctctgcc caatttttga ctatcacgtt
      181 aaaccagcat taagcagtgg gatcgagtac tagatcggat ggctggcgcg actaacccgt
      241 tcctccctta catcttgagg cgctcggcgt agccggttag ctcaacataa ccttggcctt
      301 tcacgggccg gccttgtttg gtccctgtca ctgccaccgc cccttcccaa taggagacct
      361 tggtgctgcg cgaggtgcgc aattcctgat ctgcaagcaa tggagtgacg tccagcacaa
      421 gatcgagcga cgggaacttc aggcgccatt ggttgggata cgtggccttg ctttcggcgc
      481 tggtccaggt tccggtcgat tcgatctgaa agtccgtcac ctccagatgt cgagggcgtc
      541 catccgggga cacggcagtt ccgctcgatg caagatcgga cgatccatct ttccgacgca
      601 tccggtacag catgagttcg gtatcgtcct ccaactggat gctgaaccag tcccatccga
      661 cttgatccgt cccgagttcg gccgacccga actcatggtc catccagctg gtgccggtca
      721 cgtcgaatga ctcaccatcg atcgtgagct tcccactcgt cgccagtctc gtgaacgaat
      781 agtagtgaga ggcttgaccc acctctttcc cttttcgact gatgccggcc gcgccgtgag
      841 tgaccagagg cttggccggg aggagtgtaa gagtaagtgc atgcgtcttg tcgtgggcta
      901 ccaaagtgtg tgaggcagat ggttccgtca aaacttcggc acgccaatca tcgatccaaa
      961 cacggagcct cgactcgtcg gccccggcct tccccaatcc ttcacggctg aatttttcgg
     1021 aaaaatgaaa tcgctttccg gtgatgtcgg tcacagcaaa gtgggccaga tacagatcct
     1081 tcaccgacca ttttgacgga agtgtcttaa tttcgtcagg cggaaccccg cgacgaaaga
     1141 acgtcaattc aaagccgaat gcccgaccac tctttgaatg cagatggcct gtgtagtacc
     1201 accactcggt tcgataggtc gggtgagatc catggtctcg ggggaagttg taccggtaac
     1261 cagcggttgc aaattgaaac ggcgtcggag g
//
LOCUS       DASTWE010000938         1965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_575024, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000938 DASTWE010000000
VERSION     DASTWE010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575024"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFT30_09770"
     CDS             complement(<1..449)
                     /locus_tag="VFT30_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEU5092963.1"
                     /translation="MNNSGTQRTHNTSATSVNGALADSGLICLLIIARFHDLPADGSQ
                     LRHQFAHSGQVLSDADLLRAAKHLGLKAGPFKTTWSKLLGTPLPAMAKRTDGSYIMLA
                     KVEEEKVLVQDPVEARPLVLSRGQFEATWTGELLLFTKRATVRPQDLT"
     gene            1044..1364
                     /locus_tag="VFT30_09775"
     CDS             1044..1364
                     /locus_tag="VFT30_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092964.1"
                     /translation="MATTTQYWTDENALLYQAFGTTVDWTWDYGDGTFFWSFCVRPLR
                     RQADVFVTVERVTAVEDIRTNPSAGRGGNQATILTVTVDTTSRDLRGKFTDLRFTAIK
                     VSTP"
     gene            1361..1624
                     /locus_tag="VFT30_09780"
     CDS             1361..1624
                     /locus_tag="VFT30_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092965.1"
                     /translation="MKLSLVFNSKGNIVAAARIDVESTVPLPRPVATKKLKALEVEVP
                     AEYCHCDLGTVCQSLRVDTRRKVLVPIRSKKSSKTTTRRPRSS"
BASE COUNT          439 a          510 c          535 g          481 t
ORIGIN      
        1 gtgagatctt gcggacggac agtggctcgt ttggtaaaga gcagcagttc cccggtccat
       61 gtcgcttcga attgcccacg agaaaggacg agcggccgcg cttcgaccgg atcctggacc
      121 aggaccttct cttcttccac cttcgccagc atgatatagc tgccatcggt ccgctttgcc
      181 atggcaggca gcggtgttcc caggagtttg ctccatgtgg tcttgaatgg accggccttc
      241 aatccaagat gcttagcggc acggaggaga tcggcatcgg aaaggacctg cccggagtgg
      301 gcaaactgat gccgcagctg cgagccatcg gcgggaaggt catggaatcg agcgatgatg
      361 aggagacaga tcaggccgct atcggccaat gctccattca cagaagtagc ggaagtgtta
      421 tgtgtccgtt gcgttcccga attgttcatg ggatccactg ttgtgccgac tcagctagtt
      481 gcagcaatgc ttaccatgtt ccgtgactcc aaattatgga gcaactgcgt tgcttctaga
      541 tcgttccgag catcggttcc aacggtcttt gggcgaaaag ctacgtattg gtctgcgcgc
      601 cgattgtacc agtgggtcgg aactgttcta gcttctcctt agagggggaa caatccaacg
      661 gaagtggtgg agtgggttga aacaagaaca tcgacagacg aatgaggaga agtggttgtg
      721 taccctgtgc tagtgcaggc ggagaacatc ggtcagtttc accttacagg cacgagcctg
      781 gatcaatcgt tgaggcacaa ggagttgaac ggctcattca agaaaaaagg cctcaagagc
      841 gttcgatcga tcggctgcac ggatatggtt cctttcacaa acgcgatgaa actgccctca
      901 cagcatgttc gcacgtttgg tcaaagtgac tgggcttacc cctgaagatt tctagaagac
      961 tttttcgaac ttagaaagtt ttttcgatcc ttcaggtttc ttgatgttga ctccgttgag
     1021 atcttctgtt atcaagggcg gccatggcga caacaaccca gtattggacc gacgaaaacg
     1081 cgctcctcta tcaagccttt ggaaccactg tcgattggac ctgggattat ggggacggca
     1141 cgtttttttg gagcttttgt gtgcggccct tgcgacgaca agccgacgtc tttgtgacgg
     1201 tcgagcgtgt gacggcggtc gaagatattc ggacgaatcc gagcgccggc aggggtggta
     1261 accaagctac tattcttaca gtgaccgtgg ataccacgtc gcgtgatctc agggggaagt
     1321 tcacggatct gcggtttact gccatcaagg tcagcactcc atgaagctct ccctcgtctt
     1381 taattccaaa ggaaatatcg tcgctgccgc acggattgac gtagaaagta ccgttccact
     1441 tccccggccg gtcgccacca aaaagctgaa ggcactcgaa gtcgaagtac ccgccgaata
     1501 ttgccattgc gatcttggaa cggtttgcca gagtctgcgt gtcgatacca ggcgtaaagt
     1561 gttagtcccc atccgatcga aaaaaagttc gaaaaccacc acccgccgtc cgcgatcctc
     1621 ctaaacagtc gtccatcggc aaagagggat ctcgtttcat gagcgacttc cccttattct
     1681 tgtggtccaa cctgcgactg ccgcctctgt ttctgcgggt tccatccctt tgtccgacaa
     1741 cacctcgtgc ctgaaccttt ctaaccgttc gcgattcgat caagacagag tagtctcgag
     1801 gagactgctt cagtgggtct tcacatgtcg aacggtttcg catgaaacag attcattggc
     1861 tgtatggaac ggctgtaaca attggcattg tgatatgggt cggcgcgctg gcatgttgca
     1921 ttctccttac tttccgtacc atcaatcatt tccgcgcttc ggcgc
//
LOCUS       DASTWE010000939         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1415341, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000939 DASTWE010000000
VERSION     DASTWE010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415341"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFT30_09785"
     CDS             <1..236
                     /locus_tag="VFT30_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092966.1"
                     /translation="ITLDNAITFQPFTEVHIYCLGFVSVGIKYLKTKKILYVSAWAVE
                     TSLHNVMNPLRCQLNYAFTTWLIRSWMVTEDHG"
     gene            332..>1152
                     /locus_tag="VFT30_09790"
     CDS             332..>1152
                     /locus_tag="VFT30_09790"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5092967.1"
                     /translation="MSIGNNRAVVITGASTGIGAACALHLDRLGFAVFAGVRKSEDGV
                     ALQKAGSDRLVPLELDVTDLTTIQKSHAVVLEATKKRGLFGLINNAGIAVVGPLEAVP
                     ISDLRQQLEVNVIGQVAVTQEFLPLVRQARGRIVNMGSIAGLSTMPLMGPYSASKFAL
                     EAITDALRLEVQQWGIHVAIIEPGAIATPIWNKSTIEAAEREAAIETELRTLYKPVVA
                     AVRKVVGEASKRAISSDAVAKVVEEALTAPTPKTRYLVGTDAKLRALMVKLLPDR"
BASE COUNT          288 a          279 c          319 g          266 t
ORIGIN      
        1 caattacgct ggacaacgcc atcacttttc agccgtttac tgaggttcat atctattgtc
       61 tcggctttgt atcggtagga ataaaatatt taaagactaa gaaaattttg tatgtttccg
      121 catgggcggt ggaaacatca cttcataacg tcatgaatcc gctgcgctgc caactgaact
      181 atgccttcac gacatggctg ataagaagtt ggatggtaac cgaagaccac ggctagtgtc
      241 cacaaagagg aaatggaaaa gatcatatct ctgtagagga aattgtccca aaactgatat
      301 ggtcgatttg ttgaggctcg aagagaacca catgtcgatc ggaaacaatc gtgcggtggt
      361 catcacggga gcttccaccg gaattggagc ggcctgcgcg ctgcatcttg atcgactagg
      421 gttcgctgtt tttgcaggag tgcggaaatc cgaagacggc gtggcccttc aaaaggccgg
      481 ttccgatcgg cttgttccgt tggaactgga tgtgacggat cttacaacca ttcagaagtc
      541 tcatgcagta gtcttggaag ctacgaagaa gcgcggacta ttcggactca ttaacaatgc
      601 tggaattgca gtggtggggc cgcttgaagc cgtaccgatc tccgacctcc gccagcagct
      661 cgaggtcaat gtcatcgggc aagtggcagt cacccaggaa tttctcccgt tagtccggca
      721 ggcgcgggga cggatcgtca acatgggttc catcgcagga ctctcaacga tgccgcttat
      781 gggaccctat tcagcgtcga agttcgcatt ggaagccatt acagacgcct tgcgcctcga
      841 agtccaacaa tgggggattc atgtcgcgat tatcgagcca ggtgcgatcg ccacgcctat
      901 ctggaacaaa tcgactatag aagctgctga gagagaggct gctattgaga ccgagcttcg
      961 aaccctctat aagcctgttg tggccgccgt gaggaaggtc gtcggagaag catccaagcg
     1021 agcgatctcg tctgatgcgg tagcaaaagt cgttgaagaa gcgttgaccg cgccgacccc
     1081 gaaaacgcgg tatctggtag gaactgatgc aaagcttcgc gcgctgatgg tgaaacttct
     1141 tcccgatcgg at
//
LOCUS       DASTWE010000940         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1156672, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000940 DASTWE010000000
VERSION     DASTWE010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156672"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /gene="recJ"
                     /locus_tag="VFT30_09795"
     CDS             complement(<1..1287)
                     /gene="recJ"
                     /locus_tag="VFT30_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU5092968.1"
                     /translation="MKSKLWVVRQVDPIQRGLLSQALSISSTTASLLLNRGVTNVDQA
                     TAWMSPLRRHDPFLIPDMEQAVDRLYQAMQRREVVCFYGDYDVDGMSATSIYLSFFRG
                     LGAEVRAYVPHRLREGYGLNENAVRTLAKDGVSLLVTSDCGTTSHSEIRLANQLGIDV
                     VVTDHHQSDMEMPPALAVMNPHRPEARYPFHGLCSGGLAYKVAQAYDVKYGSGSVSLE
                     SLLDLVALATIADVVPLQDENRLFVREGLAQLSRGARCGIRALKHVAGISRECTTETI
                     AFKLGPRLNAAGRLDEAIKGVRLLTTESEWEAKELAEDLERLNRARRELEAEILQEAI
                     AQVEAREWSGGIVLYARGWHLGVVGIVAARIMERFHRPTVVIAVNEHGIGKGSARTVP
                     GFDLYQTLAACRDLLMAFGGHPSAAGITIQEARLPEF"
     gene            complement(1444..1641)
                     /locus_tag="VFT30_09800"
     CDS             complement(1444..1641)
                     /locus_tag="VFT30_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEU5092969.1"
                     /translation="MEKQERKQETRREPQGKEEVKANPKVVEAGKKIKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
BASE COUNT          469 a          550 c          461 g          435 t
ORIGIN      
        1 aaattccggc aaccgcgcct cttgaatcgt aattccggcc gcgctaggat gaccaccgaa
       61 tgccatgagc aggtctcgac aggctgccaa ggtctgatac aagtcgaaac ctggcacggt
      121 ccgagcagac ccttttccga taccgtgctc atttactgcg atgacgaccg tgggacgatg
      181 gaaccgttcc atgatacggg cagccacgat accgacgact cctagatgcc atcctcgtgc
      241 atacaacacg atgccgcccg accactcccg tgcttccacc tgagctatcg cttcctgtag
      301 gatctcagcc tcgagttcac ggcgggctcg attcaaccgc tccaaatctt cagccaactc
      361 tttggcttcc cattcagatt ccgtcgtcag aagtctgact cctttgatag cctcatctaa
      421 tcgacctgcg gcattgagtc gaggtccaag tttaaacgcg atggtttccg tcgtgcactc
      481 acgactgata ccggcgacat gcttcaaggc ccgtatgccg cagcgcgccc ctcgcgaaag
      541 ttgagcgaga ccttctcgaa caaataatcg attttcgtct tgtagcggga cgacgtcagc
      601 aattgtggca agcgcgacca aatccaatag cgattccaga gacaccgaac cggagccgta
      661 ctttacgtca taggcctgag caaccttgta cgccagaccc ccggaacaaa gcccgtgaaa
      721 gggataccgc gcttcgggcc gatgcggatt catcaccgcc aaagcgggcg gcatctccat
      781 gtcgctttga tggtgatcgg tcacgacgac atcgattccc aattgattag caagacggat
      841 ctcactgtgc gacgtagtcc cgcaatcgga agtcaccaac aaggagacac catccttcgc
      901 caaggtccga acagcattct cattgagtcc atatccttca cgcaaacgat gcggaacata
      961 ggctcggact tcggcgccga gtccacgaaa aaatgaaaga taaatactcg tggcggacat
     1021 gccgtccaca tcgtaatcac cataaaaaca tacgacctcg cgtcgctgca tggcctgata
     1081 cagacggtca accgcctgtt ccatatcggg aatcaagaag ggatcatggc gtcggagggg
     1141 agacatccag gctgtcgcct ggtccacgtt cgtgacaccc cggttgagca acaatgaggc
     1201 ggtagttgag gagattgata aggcctgcga taaaaggcct cgttggattg gatcgacttg
     1261 gcgaacgacc cagagcttag acttcatccc cgcctcctta agtgtctggg ccgtctcaca
     1321 attcagacca tgtgtctcag gggaacgaga aagactgtaa ttagtcgctt cttctttgtc
     1381 aaaccgacgg ccttggggga ggcctagaat gaggctaggg ggaacagaag agatggaatc
     1441 gcgctattct cctcctttct gaacgtagtt cttcacaaat tcttcagcgt tcttttcaag
     1501 gacatcatca atttcatcga ccagcttgtc aatatcttcc ttgatcttct tgcccgcttc
     1561 cacaaccttg gggttcgcct tgacttcttc ctttccttgt ggctctcgtc tggtttcctg
     1621 ctttcgctct tgtttttcca tccgagcacc tcccggtaac caatgacagt ttcaccttaa
     1681 ccttactcca gcattcgcaa aaccgtcaag ctaacgtaga aaccatgagt cggctcttgt
     1741 cgagaaaagg agccgatcgt ctcatgcatt caggaaccgg gagaggaaca ccgctcgatc
     1801 atgaagaaag cgtcgagcag cccttttcat gcacgaacaa gcttcgagag aaacgagaga
     1861 tgctactata caatgagcga gactataagc aatgccacga tgttgatcac tttga
//
LOCUS       DASTWE010000941         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_833379, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000941 DASTWE010000000
VERSION     DASTWE010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833379"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(552..1106)
                     /locus_tag="VFT30_09805"
     CDS             complement(552..1106)
                     /locus_tag="VFT30_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5092970.1"
                     /translation="MSANAPQPTTTTWNIDPVHTTAEFKVRHMMITNVKGQFSGVTGV
                     LTLDEQDVTKSRVETSIEAASINTREADRDTHLKSADFLDVEKFPTLTFASTRITRTG
                     EGELDVEGDLMIHGVTRKVVFAVEGPTAPGKDPWGNTRIGWTATTKINRKDFGLTWNA
                     ALETGGILVGDEVTLTLDVEAVKA"
     gene            1555..1872
                     /locus_tag="VFT30_09810"
     CDS             1555..1872
                     /locus_tag="VFT30_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5092971.1"
                     /translation="MLAFSVEQRVREIGVRVALGADKREIFRLIIGNGLRLALVGAAF
                     GVPLALTLTRLMSGMVSGITGADPLTYIAVVGMLVISALLASYFPARRATRVDPIVAL
                     RAE"
     gene            1897..2457
                     /locus_tag="VFT30_09815"
     CDS             1897..2457
                     /locus_tag="VFT30_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5092972.1"
                     /translation="MNKQVLFIHGAGKGAYKEDEKLVASLRQSLDREYEVHYPAMQNE
                     DDPDYETWKRQIENNLEELDGDVIVVGHSVGASILIKFLAEGDTKKISGIFLIAAPFW
                     GGDEGWKYEGYEALALPEGHDDQLPSNAPVFFYHSRDDEVVPFAHLALYAQAFPQATI
                     RELNGRGHQLHNDLSEVAEDIKSLDR"
     gene            complement(2591..3238)
                     /locus_tag="VFT30_09820"
     CDS             complement(2591..3238)
                     /locus_tag="VFT30_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092973.1"
                     /translation="MMFQIKFKNSLVTLFGLVAFALFTAAPAYSQEKVIVTNSTSEPV
                     PTVAQGTTKVAGTILVGNTAAAPALVRDVNAASATHVGRKASEIVNLSGLFNANGEIF
                     FMRTLPDGTANPSFTIPAGKVLIITDINWQMDSGNPGTVVRLLLRIENLANPIFIRNV
                     HNSVLTLNGAGVNGVNEHLGAGIAISSAAKITAHLTAPSGALGGLFLIGYLAPEE"
     gene            complement(3581..>5188)
                     /locus_tag="VFT30_09825"
     CDS             complement(3581..>5188)
                     /locus_tag="VFT30_09825"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5092974.1"
                     /translation="TLHWANPPGGTSRRDTRPTFTSTPGPYLGPVPIVTHVHGAIGVG
                     DESDGYAEAWYLPAANNIPSGFASEGTWYNFFKGKAAASFGATWGPGFAVFQYPNENR
                     ASTIWYHDHALGMTRLNVYAGPAGFYIIRGGPDGDDAVLDSRFGTQAVLPGPAPHEND
                     KFPPNKTYYEIPIAIQDRSFNADGSLFYPNTRELFDGLTGPFIPDTDLSPIWNPEFFG
                     NVIMVNGNTWPFQRVEQRRYRLRFLNGCQSRFLIFDFSNIPGVEAWQIGNEGGFLAAP
                     VNLTSDHHNRLLMALAERADVIVDFSNVPVGNYVMANIGPDEPFGGGEPPDDFDIADP
                     ESTGQVMQFQVVPALTPDPTTPPTYLQLPAILPLPAPNNTRRLALLEEVSTVFDGPSE
                     ALLGNVDHTGTPVHKKWADPVSENPNLGDTEVWEFYNLTADAHPMHVHEIAFEVVNRE
                     RLSLENEEPVVPLELTGDVRPPEAWESGRKDTVIAYPGEVTRLRAQFNTPGQFVWHCH
                     IVEHEDNEMMRPYRIGPVQPGQPMNIE"
BASE COUNT         1208 a         1342 c         1402 g         1236 t
ORIGIN      
        1 tttgtgatct aatcccaagg ttgtcgctgc gctccaacct tgggctgaaa ttagcgaacg
       61 ccttcggcgt aattcaagaa caccttggcg taagttccaa ctgatgcact gcaagcctgc
      121 gtcgacgtcg cttgctctaa aagtcactgg atccatcttg gaggaggtca gtaccggacg
      181 cgatagcgtc gggttaattt cgccgatcgg aattccggag aattttgatg ccatcaggta
      241 gcacggtgac tatccctgta ctgacccgat ccactatatt tgaaagcggc acaacacaat
      301 gagcacacct cttaacatgc atgcggcgcg tatggcactc aaccgggacc cggaacttcg
      361 tcagtgggca gaacaatggc taaagagtaa agagcgaacg gtaaatacaa gtatgactga
      421 cgaggagttt gaaaagcact ggctctacgt tcgccctgag agaatgcacg agggcgccct
      481 ggaggcagta agcgcatacc aacaggacca cgaaggttag cttttgacga tgagttcgga
      541 tgacctgatg cttatgcctt cacggcttcg acatccagtg tcagcgtcac ctcatcccca
      601 acgaggatgc cacccgtttc aagcgccgcg ttccaggtca ggccaaagtc cttacggttg
      661 atctttgtgg tcgccgtcca accgattctt gtgttgcccc atggatcctt acccggagct
      721 gtaggtcctt cgacagcgaa tacgactttc cgcgttaccc cgtgaatcat taagtcgcct
      781 tcgacgtcca actcgccttc tcctgttcga gtgatgcgtg ttgaagcgaa ggtcaacgtc
      841 ggaaactttt cgacatcgag aaagtcagca cttttcagat gtgtatcgcg atcagcctcg
      901 cgcgtgttga tcgaagctgc ttcaatcgaa gtctcgacac gtgacttggt cacgtcttgc
      961 tcatccaacg tcaacacccc tgttacgccg ctgaattgcc ccttaacatt agtaatcatc
     1021 atgtgccgaa ccttgaattc ggcggttgtg tgaacaggat cgatgttcca ggtcgtggtg
     1081 gttggttgcg gtgcatttgc gctcatcttg tctccttttt gctccctaca tttttgcaaa
     1141 ggttatcaaa tatttattgc tttggagtca cggaaagatt cttggatttg ggtcaatcac
     1201 tgagcaggtc gctaccactc ccgtactgac ccgatccaag tgcgattccc acttttgagg
     1261 caaagccgca ccgcacgaag aatcgctctt taagaaatta atctagcatc gagcgattcg
     1321 ccgcgcgaac aatctcgacg tgcgcaatac gcgatcggat tcagctggtt gtaaacggcc
     1381 ggcctccttt ctacgaagaa aggttctaaa tttgatatcg agcaaacgtg ccatttcttt
     1441 agtcgccaga tccatctgct cctgtaatgc ttccacaaca gtgaaggcca atcctttttc
     1501 aacgtgcttg actagatcta cagagtcttt gactttaatt ccaatcagct tggtatcctc
     1561 gcgttcagtg tcgaacaacg agttcgcgag atcggcgtcc gcgttgcgct cggtgcagac
     1621 aaacgcgaga tcttccgctt gataatcggt aacggcctgc gcctcgcttt ggtgggcgct
     1681 gctttcggtg ttcccctagc gttgacattg acgcggctga tgagtggaat ggtgtccggg
     1741 ataacgggcg ccgatccatt gacgtatatt gctgtcgttg ggatgctggt gatctcggcc
     1801 ctgctggcga gctactttcc ggctcgccgc gccacacgtg ttgacccgat cgtcgcgctg
     1861 cgggcggaat aggggtattt cgttttgtct aacatcatga acaaacaagt cctatttatc
     1921 catggcgcag gaaaaggcgc ctataaagaa gatgaaaagc tggtagcgag ccttcgtcag
     1981 tcgctcgacc gcgaatatga agtccactat ccggcaatgc agaatgagga tgacccagac
     2041 tatgagacgt ggaagcgtca gatcgaaaac aacttagagg aattggatgg tgatgtcatt
     2101 gtcgtcgggc attcggtagg agcctcgatc ctaataaaat tccttgccga aggggataca
     2161 aaaaagatat ccggaatctt tcttatcgca gcgccatttt ggggaggcga tgagggctgg
     2221 aaatatgaag gctatgaagc cctggctttg ccggaaggtc atgacgacca acttccctcg
     2281 aatgcaccag tttttttcta tcacagccga gatgacgaag tagtcccatt tgcacatctt
     2341 gcgctatacg ctcaagcgtt cccacaggca acgatacgtg agttaaatgg gcgtgggcat
     2401 caactgcata atgatttgtc tgaagtcgca gaggatataa aaagtctgga tcgttagggc
     2461 tttgccttaa tagtcgaatg ttaacgtctt ttggtccaac tgccatccac gtggtccaac
     2521 aacactctaa tcttctgccg aggccaacgc ccggatgcgc tggcctcaac tgctaacgct
     2581 acaccaatgg ctattcctcc ggcgcgagat aaccgataag gaagagtcca cctaaggctc
     2641 ccgaaggagc agtcaggtga gccgtgattt ttgccgcgga ggaaatggca atgccggccc
     2701 ccaaatgctc attgactcca tttactccag cgccattgag cgtcaacaca gagttgtgaa
     2761 cattgcggat aaagattggg ttcgcaagat tctcgatcct aagtaacaaa cgtacaacag
     2821 ttccagggtt ccccgagtcc atctgccagt tgatgtccgt tatgatgagc accttgccgg
     2881 ctggaatggt gaatgatgga ttggcggtgc cgtcaggaag tgttctcata aaaaagatct
     2941 ctccgttggc attgaagagt ccgctaaggt tgacgatctc gcttgccttt ctaccgacat
     3001 gcgtggccga cgcagcgttt acgtcgcgga ccaacgcggg agccgctgcc gtattgccaa
     3061 ccaaaattgt accggcgacc ttggtggtcc cttgggccac agtcggcacg ggctccgagg
     3121 ttgagttggt tacgatgacc ttttcctggc tataggccgg ggcggcggtg aagagtgcga
     3181 atgcgacgag gccgaacaac gtaactaacg aattcttgaa cttaatttgg aacatcatca
     3241 gtctccttca gaatctgtgt ggaatttcag gcgcggcgta gtccgcgggg tttcgaggcg
     3301 cggaagatac agtgagacga atggaaagtc ttgaaactga cctgatttct ttatgaaacg
     3361 ctatgaaaga attctgagaa agacggattg gcggctgcct caaaagtcgt tggtccagct
     3421 tggatgaggt cagtacctta cgcgatagcg tagggtgcgc tccaatggca tcaaaattcc
     3481 ccggaattcc gatcggcgag attaacccga cgctatcgcg tccggttctg acctcttcca
     3541 agatcgatcc agcgactttt agggcaaagc cgtcacatac tcactctata ttcattggtt
     3601 ggccaggttg gaccggacca atgcggtatg gacgcatcat ctcgttatcc tcatgctcaa
     3661 caatgtggca gtgccaaacg aattgaccag gcgtgttgaa ctgtgcgcgc aggcgcgtga
     3721 cctctcctgg ataagcgatg accgtgtctt tgcggccgga ctcccaggct tcaggcggac
     3781 gaacatctcc ggtgagttca agcggcacga cgggttcttc gttctcgagg gacaggcgtt
     3841 cacgattcac tacctcaaag gcgatttcat ggacgtgcat cggatgcgca tcggcggtca
     3901 agttgtaaaa ctcccagacc tctgtgtcgc ccaggtttgg attctcgctg actgggtcgg
     3961 cccacttctt atgcactggc gttccggtgt ggtccacgtt acccagcaga gcctctgagg
     4021 gtccgtcaaa aactgtcgac acttcttcca gaagcgccag ccgtctcgtg ttgtttggtg
     4081 caggcaaagg taggatcgcc ggtaattgaa gatacgttgg tggcgtcgtg ggatccggcg
     4141 tcagtgcggg gacgacctgg aactgcataa cctgtcccgt actttccgga tcggcgatgt
     4201 caaagtcatc cggcggctcg ccaccgccga aaggttcatc tggtccaatg ttggccatga
     4261 cgtaattacc aacaggaacg ttggaaaagt ccacaataac atcagcccgc tcggcgagcg
     4321 ccatcagcag acggttatgg tgatcagaag tgaggtttac tggcgccgcc aggaagccac
     4381 cctcgtttcc gatctgccac gcttccaccc cgggaatgtt gctgaagtca aagatcagaa
     4441 atcgcgattg gcaaccattc aaaaagcgta agcggtagcg ccgctgctcg actctttgga
     4501 aaggccaggt gttgccgttc accataatca cgttgccaaa gaactcggga ttccagattg
     4561 gagagagatc tgtatcggga atgaatggtc cagtgagccc gtcgaacaat tcacgggtgt
     4621 tcggatagaa gagcgatcca tcagcattaa aggatcgatc ctgaatagca atcggaatct
     4681 catagtaggt tttgtttggg ggaaacttgt cgttctcatg aggagcaggt cccggcaata
     4741 ccgcctgcgt accgaatcga ctgtcgagga cggcgtcgtc gccatcggga ccaccacgga
     4801 tgatatagaa cccggcgggt ccggcgtaca cattcaggcg agtcatgccg agagcgtggt
     4861 cgtggtacca aatagtcgag gcccggtttt cattcggata ctggaaaaca gcgaatcccg
     4921 gaccccaggt cgctccaaaa cttgcagcag ccttaccctt aaagaagttg taccaggtgc
     4981 cttcagaggc gaaaccgctc ggaatgttat tggcagccgg caggtaccag gcttccgcat
     5041 agccgtctga ttcatcaccg acaccaattg cgccgtgaac gtgggttacg atcggcactg
     5101 gaccaaggta tggaccaggt gtgctggtga aggttggccg agtgtcccgg cgcgatgtgc
     5161 caccaggcgg gttggcccag tgcagcgt
//
LOCUS       DASTWE010000942         1460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1156784, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000942 DASTWE010000000
VERSION     DASTWE010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156784"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1011
                     /gene="kdsA"
                     /locus_tag="VFT30_09830"
     CDS             184..1011
                     /gene="kdsA"
                     /locus_tag="VFT30_09830"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU5092975.1"
                     /translation="MVHLVDIGSFKVGQGERPFLIAGPCVIESEQLVMDTAGRVAEMT
                     KALGVPYIFKSSFDKANRTSVKSFRGPGIKDGLTILKKVKDHFDLPVLTDVHTEEQAT
                     EAGKVVDVLQIPAFLCRQTDLLIAAAQTGKVVNVKKGQFLSPREMGNAIQKIEECGSQ
                     RILLTERGSSFGYNNLVVDMRSFPIMRNFGYPVVFDATHSVQLPGGGGTKSSGQREFV
                     EPLACAAAGAGVDGFFMEVHPNPDVALSDGPNMVPLHQLKFLLERVLRICDAAKSKS"
     gene            1095..>1460
                     /locus_tag="VFT30_09835"
     CDS             1095..>1460
                     /locus_tag="VFT30_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5092976.1"
                     /translation="MTVKLSGRELKATKMEESLADGRRVLEIEARAVQSLVNRLDAKF
                     AKAVDLLVQCKGKVVVSGMGKSGLIGQKIAATLASTGTSSFFLHPAEGVHGDLGMLAR
                     RDALVAISNSGETQELLQLL"
BASE COUNT          341 a          370 c          425 g          324 t
ORIGIN      
        1 ccaccctgaa tacagttccc gtccgcaccg tccgcatcct ttattcagtg ggttcgtcgg
       61 ggcagcgttg cgaaaaaagc tgggacactg aagataaagg tttagactaa ggctgagtat
      121 aggagaagtg ctttccaacg ttcctttcgt cttgacctca accttaacct ctattgagca
      181 gctatggtac atctcgtcga catcggttct ttcaaggtcg gtcagggaga gcgccccttt
      241 ctgattgccg gcccttgtgt catcgagagc gagcaactgg tgatggacac ggcggggcga
      301 gtcgctgaga tgacgaaggc gctgggggtg ccatatatct tcaaatcatc attcgacaaa
      361 gctaaccgta cctccgtcaa gtcgtttcgt ggtcctggga tcaaagatgg tctgaccatc
      421 ctaaagaagg tgaaggatca ctttgatctg cctgtcttga ccgatgtgca cacggaagag
      481 caggcgacgg aagccgggaa ggtcgtggat gttctccaga ttcctgcctt tctctgtcgg
      541 cagacggatt tactgattgc cgccgcacaa accggaaaag tcgtgaatgt gaagaagggg
      601 cagtttctct cgccgagaga aatgggcaat gcgatacaga agatcgaaga gtgtgggagt
      661 caacggattc tgctcaccga acgagggtca tctttcggct acaacaatct tgtcgtggat
      721 atgcgatcct ttcctattat gaggaacttc gggtatcctg tcgtcttcga tgcgacccac
      781 agtgttcagt tgcccggtgg tggtgggaca aaatccagcg gccagcggga atttgtcgaa
      841 ccgttggcgt gcgccgcggc cggagcaggg gtcgacggat tcttcatgga agtccatccg
      901 aatcccgacg tggccctgtc cgatgggccg aatatggtac cgttgcatca attgaagttc
      961 ttgctggaac gggttctacg catatgcgac gcagcaaaat caaaaagctg aagccatccg
     1021 cgcgacgcag tcaacctacg aaggtgaaga cagccggtcc agtatcggag gctgcgaaga
     1081 ggcgcaacaa aaccatgact gtgaagctat ccggccgaga attgaaagcc acgaaaatgg
     1141 aagaaagcct tgccgacggt cggcgtgtcc tcgaaatcga ggctcgagca gttcaatcac
     1201 ttgtgaatcg gctcgatgcc aagttcgcca aagcggtgga tctgcttgtt caatgcaagg
     1261 gaaaggtcgt cgtctccggc atgggaaagt cgggcttgat cggacagaag attgccgcaa
     1321 cgctcgccag caccggcacc tcctcgttct tcctacatcc cgcagaaggc gtgcacggag
     1381 atctgggcat gttggctcgt cgggatgcgc tggtcgcgat ctccaacagc ggcgagacgc
     1441 aagagttgct tcaattgctt
//
LOCUS       DASTWE010000943         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_380707, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000943 DASTWE010000000
VERSION     DASTWE010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380707"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1126)
                     /locus_tag="VFT30_09840"
     CDS             complement(<1..>1126)
                     /locus_tag="VFT30_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5092977.1"
                     /translation="TITLKDQRSPVWEYRNILRKGLSERYPGTEFTFQPADLTDKILN
                     FGSLAPIDLQINGPDLYANYAYAQKLAGKFRQIAGAADVYIYQTMFQPTILVEGDRRF
                     ALGMNLNQKDFGFNMLLATAGSQQIDQKYWLDRSTQQSYRLNVYTPQPQLTSVKDLKT
                     IPVAPEMREHGEDSEENLQLLGNMTKLSLIGTPGVVTHRNLLPMIDLLVSAEGRDLGG
                     VLADVRKIAESMRDELPRGSEVEIHGQSEVMHDAFVELGGGLIIAILLVYLLIVVNFQ
                     SWLDPFIIITALVGAFAGIAWSLFATHTNLSVPALTGAIMTMGTATANSILLVAYARE
                     RLAVHGGALRAAVEAGKGRIRPVLMTAAAMIIGMLPMAMAN"
BASE COUNT          258 a          301 c          316 g          251 t
ORIGIN      
        1 agttggccat tgccatcgga agcatgccga tgatcatggc cgcagcggtc atgagtaccg
       61 ggcgaatgcg acccttgccc gcctcgaccg cggctcgcag cgcaccaccg tggacagcaa
      121 gccgctcacg agcataggcg acgagcagga tcgaattggc ggtggccgta cccatcgtca
      181 tgatggcgcc ggttagagcg ggtacggaaa gattggtatg ggtcgcgaag agtgaccacg
      241 caatgcccgc gaacgcgccc accaaggccg taataatgat gaagggatca agccaagact
      301 ggaaattgac gacgatcagg agatagacca agaggatggc gatgatgaga ccaccaccta
      361 gctcgacaaa agcgtcgtgc atgacttcgg attgaccatg gatttcaact tcagagcctc
      421 gcggcagctc atctctcatg ctctccgcta ttttccgcac atccgccagc acaccaccga
      481 ggtcgcgtcc ctccgcggat acgaggaggt cgatcatcgg caggagattt cggtgcgtca
      541 ccacacccgg cgtcccgatc aaggaaagtt tggtcatatt accgagcaat tgcagatttt
      601 cttccgaatc ttccccatgt tcgcgcattt ctggtgcgac tgggatcgtc ttgagatctt
      661 tgacgctcgt cagctgtggt tgaggggtat agacgttgag ccgataagac tgttgtgttg
      721 aacggtcgag ccaatatttc tgatcaatct gttggctgcc ggccgtcgcc aggagcatgt
      781 tgaagccaaa gtctttctgg ttcagattca tgccgagcgc gaatctccgg tcgccttcga
      841 ccaggatcgt cggttgaaac atggtttgat agatatacac atcggcggcc ccggcgatct
      901 gccggaattt gcccgccaat ttttgagcat acgcataatt ggcatagagg tcaggcccat
      961 tgatctgtag gtcgattggc gcgagcgagc caaaattgag aattttatcg gtcagatcag
     1021 ccggctgaaa cgtgaattcc gttcctggat agcgctccga caaacctttg cgaagaatgt
     1081 tccgatattc ccacaccggt gacctttgat ccttcaaggt aatggt
//
LOCUS       DASTWE010000944         1368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_962777, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000944 DASTWE010000000
VERSION     DASTWE010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962777"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFT30_09845"
     CDS             complement(<1..1104)
                     /locus_tag="VFT30_09845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU5092978.1"
                     /translation="MMNELFFSSMTALLVCMALIPPLRIAAERFQVMDLPGERKVHEH
                     PVPRVGGLAFAVGACIAIAWWGARDATTFSVLLGCVIIVAFGVWDDRVDLSYRSKLVG
                     QLLASFAVVWGGDIWFTTLPFLSDVEMPVWAGMFVTVVFLVGVSNAVNLTDGLDGLAG
                     GLSFLTLSGIAYLAYLSNDSTVLLLTVPFLGGLLGFLRYNTYPARIFMGDGGSQLLGF
                     IMGVLAILLTDSSRGPFSPSLAFFLLGLPFLDTLGVTGQRLAEGRSPFMGDRAHIHHK
                     LLRFGFTHYEAVTVIYTIQAGMLGLAFLLRWQSDTLILPLYLMIAGSVLMLFIAAGRG
                     LLPNLTSQSGHFLSTVAVTRLMNGPWLTDLPMQF"
     gene            complement(1136..>1368)
                     /locus_tag="VFT30_09850"
     CDS             complement(1136..>1368)
                     /locus_tag="VFT30_09850"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEU5092979.1"
                     /translation="KQIVLYWFKQRDRNLTNEYLVKLYLLWDAFSRQRTDGALVRLAT
                     VVGPGESESVVDRRLQQFAAAVGRELSRFVPD"
BASE COUNT          399 a          411 c          324 g          234 t
ORIGIN      
        1 aaactgcatc ggcagatccg tcagccacgg cccattcatc aatcgcgtaa cggccacagt
       61 ggataggaaa tgaccgcttt gtgatgtgag attgggaagg agtcctcgcc cagctgcgat
      121 gaacagcatc aggaccgatc ccgcaatcat aagatagagc ggcaggatca acgtgtcgga
      181 ctgccatcga agcaaaaacg ccaagcccaa cataccggcc tggatcgtgt agatgaccgt
      241 cacagcctca tagtgcgtaa atccgaaacg aagcagctta tgatggatat gcgcacggtc
      301 acccatgaag ggagaacggc cttcggccag ccgttgtccc gtcacaccga gagtgtccag
      361 aaacggcaat cctaacaaga agaatgcaag gctgggactg aacggaccgc gtgacgaatc
      421 agtcaataag atagccaaca cgcccataat aaatcccaac aattggctac ccccgtcacc
      481 cataaaaatt cgggccgggt atgtgttgta gcgcaaaaac cccaagagtc ctcccaaaaa
      541 tggaaccgtt aacaacagta ccgtcgagtc attggagaga taggccaaat aggcgattcc
      601 agatagagta agaaacgaca gaccgccggc taacccatcc aacccatccg taagattgac
      661 ggcattggac acgccgacaa gaaacacgac ggtcacaaac atcccggccc ataccggcat
      721 ctccacatca gatagaaaag gaagagtggt aaaccagata tcccctcccc aaacgacggc
      781 gaaggaggcc aacagctgtc caactagttt gcttcgatag ctgagatcga cacggtcgtc
      841 ccacactccg aacgccacga taatcacgca tcccagcagc accgaaaagg ttgtcgcatc
      901 tcgagcaccc caccaggcaa tcgcaatgca cgcgcccacg gcaaatgcaa gcccacccac
      961 ccgtggaacc gggtgctcat gcaccttccg ctctccgggt aaatccatca cctgaaaccg
     1021 ctcggccgct atccgcaagg gaggaattag cgccatgcac accagcaacg cggtcatgga
     1081 actgaaaaag agctcattca tcatggctac actcgcagat catgctcttc gaccgtcagt
     1141 ccggcacaaa ccgactcagt tctcgtccca cggcagccgc aaattgctgg agacgtcgat
     1201 caacgacaga ctcggattca ccagggccaa cgaccgtagc caacctcacc agcgcaccgt
     1261 cggttcgttg tcgagaaaat gcatcccaca gcaagtacaa cttgaccagg tattcgtttg
     1321 tgagatttcg atcgcgttgc ttaaaccagt acaagacgat ttgttttt
//
LOCUS       DASTWE010000945         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_542820, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000945 DASTWE010000000
VERSION     DASTWE010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542820"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..514
                     /locus_tag="VFT30_09855"
     CDS             296..514
                     /locus_tag="VFT30_09855"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092980.1"
                     /translation="MAEGKKVRIRVRTVNCTYVGDFLVPPMRNRVSDAINEEARLFIS
                     LTDVLIDDKERLDFVAINKNLIESIAQL"
     gene            632..2332
                     /locus_tag="VFT30_09860"
     CDS             632..2332
                     /locus_tag="VFT30_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5092981.1"
                     /translation="MRPYLARLILAGLLVSGVALINLALVRLAGTLWDIITVQHDADK
                     MRRSIGLFLGLVILQGLCAMGHSYLTAWVSQEVVADFRKHLFGHLQTLTVSFFARRRT
                     GELLSRLMTDVTVIQSIVTETPIDGIKQLVTFVGGITFLLMMNWQLCILILILLPLLV
                     LVAKVFGRRLKSLSTSIQDHTAALSTLVEEVISGIRIVKSFVQTQREKTRFADQVDQT
                     LHLTLHRAGIMAVFIPVISLLTFSSATAVLWYGGRQVIDGVVTPGDLFAFVLFAGILI
                     GPFSSAARVFAQIKEAQGATQRVFEILDAQPDISDQSDAQTLVTVDGHVRVEGVNFSY
                     DARHPVLSNLSFEAKPGELVALVGPTGAGKTTVINLLHRFYDPTEGRIMIDGKDLRHV
                     TVESWYRQIALVPQETILFGGTILDNIRYGNMTANEAAVLEASKAAHAHDFITSLPDG
                     YQTMVGEKGVNLSGGQRQRIAIARAILKNPRILLLDEATSSLDTESERLVQEALQRLM
                     EDRTTFVVAHRLSTIQRADRILVFDKGKLVEEGNHAQLMVRNGLYHYLYTVRLNEPIT
                     "
     gene            2438..>2777
                     /locus_tag="VFT30_09865"
     CDS             2438..>2777
                     /locus_tag="VFT30_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5092982.1"
                     /translation="MASQRPKKILIVEDEQDIAQLVRHYLEKEGYHPNIAKTGLEALN
                     LAVSEHPDLIILDLMLPQMDGLEVCKALRHKPETALLPIIMLTAKKEESDTVVGLELG
                     ADDYITKPFSP"
BASE COUNT          652 a          756 c          724 g          645 t
ORIGIN      
        1 aactaccacc acggcgagca ttaggagcca caggatggat gagggccatg aaaaaccgat
       61 ccttcgccta aacatggcga tgcagtataa cacaaggcaa aatggcgccg tgaacttgca
      121 ttttcagcgg aaagctttta gactcggcgc tccctaggag atacagaaca tgtcaagcgt
      181 cttgaaaaag cgacgaaaga agatgcgcaa gcacaagtac aagaagctgc gccagcgtca
      241 gaagtttctt cgcagaaaaa gttaagctca gccggcgtgg cgggaggagg aaaccgtggc
      301 cgaggggaag aaagtccgca tccgcgtacg gacggtcaat tgtacctacg tgggagactt
      361 cctggtccct ccgatgcgga atcgtgtctc cgatgcgatc aacgaagaag cgcgtctctt
      421 catcagcctg accgacgtct tgatcgacga caaggagcgg ttggatttcg ttgcgatcaa
      481 taaaaacctg atcgagtcga tcgctcaact ctagtcgttt cgtcttaccc cgttactcat
      541 tctttcaccg tcgccagccg gaacacgatt ccgtgtccaa tcacaccgcc tgattactat
      601 gatgttgatc ttcaaacgat tcctcccgtt cgtgcgcccg tatttggcgc gtttgatact
      661 ggccggcctc ttggtgagcg gggttgcgct tattaatctc gccttggttc ggttggccgg
      721 caccctctgg gatatcatca ccgttcagca cgacgccgat aagatgagac ggtcgatcgg
      781 cttgttcctg ggccttgtaa tcctgcaggg actatgcgcg atgggtcata gttatctgac
      841 cgcgtgggtc tctcaggaag ttgtcgctga ctttcgcaaa catctcttcg gccacttgca
      901 gacgctgacg gtcagctttt ttgctcgacg tcgtacggga gaactgctct cccgattgat
      961 gacagatgtg acggtcatcc aatccatcgt gacagaaact cccatcgacg gcatcaaaca
     1021 actcgtgacg ttcgtcgggg gtatcacgtt cctacttatg atgaattggc agctctgcat
     1081 cctaatcctg attcttcttc cgttgctggt tctggtcgcc aaggtattcg gccgcagatt
     1141 gaaatctctt tccacctcga ttcaagacca cacagccgcg ctcagcaccc tcgtcgagga
     1201 agtgatttcc ggcattcgta tcgtcaaatc gttcgtccag acacaacggg aaaagactcg
     1261 tttcgcggat caggtcgatc aaacgcttca tctcacactc catcgagccg gaatcatggc
     1321 cgtcttcatt ccagtgatca gcctcctgac gttctcgtcg gcaacggcgg tgctgtggta
     1381 cgggggcaga caagtcatcg atggggtcgt tacgccgggc gatctgttcg ctttcgtctt
     1441 gttcgccggc atcttgatcg gccccttcag ctcggcggcg cgcgtctttg cgcagatcaa
     1501 agaagcccag ggagcgacgc aacgtgtctt cgaaattctg gacgctcagc cggacattag
     1561 cgatcaatcc gacgcgcaga ccctggtaac cgtcgatggc catgtgcgag tggaaggcgt
     1621 caacttcagc tatgatgctc gccatccggt attgtcgaac ctgtcgttcg aagccaagcc
     1681 aggcgaattg gtcgcgctag taggaccgac gggtgccggc aagaccaccg tgatcaacct
     1741 gcttcaccgc ttctacgatc caacggaagg tcgcatcatg atcgacggca aagacttgcg
     1801 ccatgtgacc gttgagagtt ggtaccgaca gattgctctc gtcccacaag aaactatcct
     1861 ttttgggggc accatcctcg acaatattcg ctacggcaat atgacagcga atgaagctgc
     1921 agtgctggaa gcgagcaaag ctgctcatgc ccatgacttt atcacgagct tgcccgacgg
     1981 gtatcaaacc atggtgggtg agaaaggggt caatctctcc ggtgggcaac gccaacgaat
     2041 cgcgattgcc cgggcgatct tgaagaaccc acgcattttg ttgcttgacg aagcgacctc
     2101 gtcgctggat actgaatcgg aacggctggt tcaggaagcc cttcaacgcc tcatggaaga
     2161 tcgcacgacc ttcgtcgtcg cccaccgact gtcgaccatt cagcgtgccg accgaatttt
     2221 ggtttttgac aaaggcaaac tggtggaaga gggcaaccac gcccagttaa tggtccgtaa
     2281 cgggctgtac cactatctgt ataccgtccg tttaaacgag ccgatcacct gaaacatcgt
     2341 cttcagcacg aacttacctt ccgatatttc taaccattta acaggcctgt aacagaaaga
     2401 gcgtatggtt ggccagctga cacacaaagg tggtggtatg gcttctcaac gtcccaagaa
     2461 aattctcatc gtcgaagatg aacaggatat tgcgcagttg gtgaggcatt atcttgaaaa
     2521 ggaaggatat catccgaaca tagctaagac cggtcttgaa gccctcaacc tcgcggtatc
     2581 tgagcatcct gatttaatta ttcttgacct catgctgcct cagatggatg gattagaagt
     2641 atgtaaagct ctccgtcata agcccgagac ggcgctgttg cccattatca tgctgacggc
     2701 gaagaaggag gagtccgata ccgttgtcgg acttgagctc ggagctgatg attacatcac
     2761 taaacccttc agcccca
//
LOCUS       DASTWE010000946         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_736317, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000946 DASTWE010000000
VERSION     DASTWE010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736317"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..587)
                     /locus_tag="VFT30_09870"
     CDS             complement(333..587)
                     /locus_tag="VFT30_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092983.1"
                     /translation="MTLQGRLTRRSISIPESPADRGRSLYVLLVSDALIGNGLLGIGS
                     SRALSHHFDHIRHEMKSTHIVEEYHVNRRISGTFFLHHDP"
     gene            586..1176
                     /locus_tag="VFT30_09875"
     CDS             586..1176
                     /locus_tag="VFT30_09875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5092984.1"
                     /translation="MSEPHQELWLIRHGETEWSAAKRHTGRTDISLTPTGERQAAALG
                     KSLAGRRFELVLCSPLRRASETCRLAGYGQAAEVTDDLLEWDYGVYEGRTTQEIRLEQ
                     PGWSIWRTSVTKGESIEHVGHRAQRVIQKALAVDGDVVLFAHAHILRILAARWLDLPP
                     DAGRLFVLRTASISVLGFERETRVITRWNLLHEGRV"
     gene            1173..1619
                     /locus_tag="VFT30_09880"
     CDS             1173..1619
                     /locus_tag="VFT30_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5092985.1"
                     /translation="MKAHYLGHVVFYVKNLERSLEFYRDLLGFTQVGQIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPRGPRRGLYHIGIKVGDSLDELRSAKQELEQAGVTIDGM
                     SDHTVSQSLYLKDPDGNEVELYVDADESLWKNNSAAVLSPIKPLHL"
     gene            1970..>2284
                     /locus_tag="VFT30_09885"
     CDS             1970..>2284
                     /locus_tag="VFT30_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092986.1"
                     /translation="MDDAGLVNKPLGAWTKWALVWLTVFPFVGILVSIRFHNREFLGG
                     MPLFTFGRLRSVHLGGVLFGPFTSALIAIFYYCSAVRHAAVLLPYGPVNGVNSAAIHG
                     LCM"
BASE COUNT          508 a          574 c          675 g          527 t
ORIGIN      
        1 gctgggtgcc gaagcggtag tcacgatcga agtggagtga taaccgggcc gatcgaaggg
       61 atcggctgcg cgctcttcat gatcgggtaa gggtcatgga attcactggg caaagctaga
      121 ggacatctcc gccattctcg atttacaggc ggcgaacttc atcggcaacc tggaggatac
      181 ggaacgccag gattgctttt ttcgtcggtg acatttaatc aatattccct tcgtgtccat
      241 gtggatggac ttgggatggc tcatgtcggg gaatttgtct tctcagcgta gcagaatcac
      301 attctgatgt ttgatgcgcc gagcaagaca atttatggat catgatgcaa gaagaaggtc
      361 ccactgatgc gccgattgac gtgatattcc tcaacgatgt gggttgattt catctcatgg
      421 cggatgtggt cgaaatggtg ggacagggct cgactgctgc ctatgccaag caagccattg
      481 ccgatcagcg catcggacac aaggagtaca tagagactac ggccacgatc tgccggagat
      541 tcgggaatgg aaatggagcg gcgagtaagc cgcccctgaa gagtcatgag cgagccacac
      601 caagaacttt ggttgatccg acatggtgaa acggaatgga gcgccgccaa gcggcatacg
      661 ggccggactg atatctctct gacgccgacg ggggagcgtc aggctgccgc gttagggaag
      721 tctctggccg gtcgacgatt tgaattggta ctctgcagcc cgttgcgccg ggcgagcgag
      781 acctgccggc tggccgggta tggccaggcg gccgaagtga ccgatgacct gctcgaatgg
      841 gattatggtg tttatgaagg aagaacgacg caggaaatca ggttggaaca gcctgggtgg
      901 tcgatctgga ggacttctgt caccaaaggc gagtcgatcg agcatgtcgg gcaccgcgcc
      961 caacgggtga tccagaaggc gctggcggtt gacggcgatg tggtcctatt tgcgcatgcc
     1021 catatcctaa gaatattggc cgcgcgctgg ctcgatcttc cacccgatgc tgggcggttg
     1081 ttcgtgctcc gcaccgcctc gataagcgtg ctcggcttcg agcgtgaaac gcgcgtcatc
     1141 actcggtgga acctcctgca cgaggggcgg gtatgaaagc acactatttg ggacacgttg
     1201 ttttctatgt gaaaaatctt gaacgatcgc ttgagttcta tcgagaccta ttggggttta
     1261 cccaagtggg ccagattttc aatggagcag ccgctgctct gacatcaggc cggacgcatc
     1321 acgaattgtt gttgatccaa gtcggcgatg ctcccggtcc gccgaggggg cctcgtcgag
     1381 ggctttatca tatcggcatt aaggtgggag acagtttgga tgaactccga agcgccaagc
     1441 aagagcttga acaggccggc gtaacgatcg acggcatgag cgatcacacg gtgagccaga
     1501 gtctttatct caaagatcct gacggcaacg aagtggagct ctatgtcgat gccgatgagt
     1561 ccttgtggaa gaacaactcc gcggccgtct tgtcaccgat caagccgcta catctgtaat
     1621 ctagagaggc agacagatca tggcagccaa gccaccggca gttctgttaa tggccgggga
     1681 acctactgtt gatgtcgctg cggacatccg agagacccgc ctatggggca tcgcacgaag
     1741 aaacagattg gagacggttg agtttatgat gaatgaaaga aacaagtggt gggtgctact
     1801 tgcgtgcggg gccaaacatc aaaccacatt tttacaattc cacgcaatgc aaaactttcc
     1861 tgtctgttga tcaagcccat cttcgctcct atagtcgaaa taaatccact tcagtcatta
     1921 aaaaggtggt gctctgcaca agctcgttcc ctttctgagg taactcagta tggatgacgc
     1981 cggacttgtc aataaaccgc tgggagcctg gactaaatgg gcgctagtct ggctcacagt
     2041 cttcccattc gtcggcatcc tcgtttctat tcgattccat aatcgcgaat ttctgggcgg
     2101 catgcctttg ttcacgttcg gccggctacg ctctgtgcat ctagggggtg tgctgttcgg
     2161 gccattcaca tcggctctca ttgctatctt ctattattgt tccgctgtgc ggcacgccgc
     2221 cgtgctcttg ccctatggcc ctgtgaacgg cgtgaacagc gcagcgatcc atggcctgtg
     2281 catg
//
LOCUS       DASTWE010000947         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_865772, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000947 DASTWE010000000
VERSION     DASTWE010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865772"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFT30_09890"
     CDS             <1..625
                     /locus_tag="VFT30_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5092987.1"
                     /translation="MSASPTIRRSPASSSSDPHSAKNFDTLYKDHVDLMYRFAHRLCG
                     EPEAAKDLVQETFLNAYRGFDKFRGDAQISTWLYTIASRACLRMRRRRKGAPERELSL
                     EEFIPSSDGEFRLQIPIEGLSPEAALQNKQLREALDAAINQLPKKYKMVLVLRDMEGL
                     SAKEVGAIMGLNERAVKSRLHRARLFVRRELSARGLGEAPSDHDTLG"
     gene            974..>2344
                     /locus_tag="VFT30_09895"
     CDS             974..>2344
                     /locus_tag="VFT30_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092988.1"
                     /translation="MNKNLLLGIVLVSGLLTAFEAILPDLGTAASKSPQKATENKAAI
                     NTALRYAEAIAKGDKITAGQLDFACQYKHLTVPSANPPNPQATEASYDGCWQELTAGH
                     ASVLKRSDIGMNILWPSTGPLVFYGDDLPRTQASAFVMDVLGMSPPGTGLHLTVANSR
                     SIPNGSFRLKPKEKIIGVPTTLVDLTVHYHDPLTSPVTYGPDTVRWTNTIKRQRRAIK
                     SITTQWVVFTGLKKYGFPRDSAVFHLPVRTKPEAPGMVVDRVPFTTDTSRALPDSLVW
                     WGSDDQPGTLTAAATQAAAFPELRDRIAMLNRILIIDPHQVDALTILTRNLHAALLRE
                     AVQIHKLNVKDPGLSLTVNEFYWNIYAAAGRLDLANAMEMGGFSQPTPADFLYRLLPA
                     LETLSKTRPERLDNRFWLGMAYRWNNDQIPMIETFEALVRDIPADKKTPRAEALVQLA
                     WSRINKV"
BASE COUNT          531 a          692 c          625 g          496 t
ORIGIN      
        1 tatgagcgcc tcaccgacca ttcgccgctc gccggcctcg tcctcgagcg atccccactc
       61 cgctaaaaat tttgatactc tatacaagga ccacgtagac ctcatgtatc gcttcgctca
      121 tcgtttgtgc ggggaaccgg aggctgcaaa agatttggta caagagacct ttttgaatgc
      181 gtatcggggc ttcgacaaat ttcggggtga cgcccagatt tcgacatggc tctataccat
      241 cgcctctcgg gcatgcctac gcatgcggcg cagacgaaaa ggagccccgg aacgagagtt
      301 gtccctcgag gaattcatcc cctcctccga cggtgaattt cgtttgcaga ttccgataga
      361 gggactcagc cccgaggccg cgctccaaaa taagcaattg cgggaagcgc tcgatgccgc
      421 gatcaaccaa ctaccgaaga agtataaaat ggtcttggtg cttcgtgaca tggaagggtt
      481 aagcgccaag gaggtcggag ccatcatggg cttgaacgaa cgagcggtga aatcgcgctt
      541 gcaccgggcc cgactgtttg tgcgccgtga gctcagtgcg agaggcctcg gcgaagcgcc
      601 gagcgatcac gatacacttg ggtagcggag ggactgatcc acatggtaaa acgtagccct
      661 tctaagcggc gacggcattc ttcaacgact cggcaccgac atactcatgg cacaagccgg
      721 tgcctgcgca tccttcgccg gttatccgct tatatcgatg acgagctttc cgcgagtatt
      781 tgccaagaaa ttcgtcgaca tctgggcgct tgtccaaatt gtgaaacctt cgtgatgtcg
      841 ttgcgccaaa ccgtgtcgct ctgtcgccac agtcctgcac cgactttatc gactgccgac
      901 cgtgcgttga tgcgtgcgaa gatattgaaa accgcctaat ctgggtaacc cacaacaccg
      961 tgagtgtttc atcatgaata agaatttatt gcttggaatt gtactcgtct cgggactgct
     1021 gacggccttt gaggcgatat tgcctgacct tgggactgct gcgtccaagt ctcctcagaa
     1081 ggcgacggag aacaaagccg ccattaacac agcccttcga tatgccgaag ccatcgccaa
     1141 aggcgacaag atcacggctg gacagctgga cttcgcgtgc cagtacaagc acttgaccgt
     1201 gccgtcggcc aatcctccga atcctcaggc aactgaggcc tcgtatgacg gttgttggca
     1261 ggaattgacc gccggccatg cctcggtctt gaagcgatcg gatattggga tgaacatcct
     1321 ctggcccagc accggacctc tggtatttta tggagatgat cttccccgga ctcaagcctc
     1381 agcctttgtc atggatgtcc tcggaatgtc cccccccgga accggccttc atctcacggt
     1441 ggcaaacagc cgttcgattc cgaacggttc attccgactg aagccgaagg agaaaatcat
     1501 cggcgtcccg accacgctgg ttgacttgac cgtccattac cacgacccgc tcacatcgcc
     1561 ggttacgtac ggaccggaca ccgtccgatg gacgaatacg atcaaacgac agcggcgcgc
     1621 cataaaatcc atcacgactc aatgggtggt cttcaccgga ctcaaaaaat acggctttcc
     1681 tcgagactcg gccgtctttc atttgcccgt gagaaccaag cctgaagcgc cgggaatggt
     1741 cgtggacaga gttccgttca caaccgacac gagcagggca cttcctgact cgctggtctg
     1801 gtggggttcc gacgatcaac cgggaacctt gacggcggca gcaacacaag cggccgcatt
     1861 tcctgaatta cgcgatcgca tcgccatgct caaccgcatc ttgatcatcg acccgcatca
     1921 ggtcgacgca ctcacgatcc tgaccagaaa tctccacgcc gccttgcttc gggaagccgt
     1981 tcagatccac aaactgaacg tcaaggatcc tgggctatcg ctgaccgtca acgagttcta
     2041 ttggaacatc tatgcggccg ccggccggct cgatcttgcg aatgctatgg agatgggtgg
     2101 cttctcgcaa ccgacaccgg ccgatttcct gtaccgactg ttgcccgcac tggaaaccct
     2161 gtcgaaaacc cgtcctgagc ggttggataa ccgcttttgg ctcggcatgg cttatcgctg
     2221 gaacaatgac cagattccga tgatcgagac gttcgaagcg ctggtcagag acattcccgc
     2281 cgacaaaaag acgcccagag ccgaggcgct ggtgcagttg gcctggtcgc gtatcaacaa
     2341 ggtg
//
LOCUS       DASTWE010000948         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_25761, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000948 DASTWE010000000
VERSION     DASTWE010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25761"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..>1215
                     /locus_tag="VFT30_09900"
     CDS             251..>1215
                     /locus_tag="VFT30_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU5092989.1"
                     /translation="MRGIDHQQADMYSYISPEARVRGDHPLRKIRTMADEALNNMSAR
                     FDAMYAKIGRPSIPPEKLLRAQLVQMLYSIRSERLLMEEIDYSMLFRWFVGMNLDEPV
                     WDATVFTKNRNRLLEGDVAREFLCEVVKQAQSNGLTSDEHFTVDGTLIEAWASLKSFQ
                     RKDQNNQPPDDPGNPTVNFHGEKRSNQTHESTTDADALLARKGNGKEAKLSYNGNLLT
                     ENRNGLIVNTEVFPANGMAERAAAMAMLEQVRGDHRVTVGADKGYDTRDFVAECRHLH
                     VTPHVAQNTKRSGGSAIDARTTRHGGYAVSQKKRKRVEECFGWMKT"
BASE COUNT          309 a          318 c          375 g          213 t
ORIGIN      
        1 cggctccctc caccgcccaa cggcgcgagg aaaatctggc tccgaactcg tgcaatcggc
       61 tcagaccttc cggggtgttt ggcacgacca aatgccccaa agacgcgcca ttttcatcaa
      121 gagcaacaac ggtgtgagcg ccgggatgcg gatcaagacc gagagtgatc atctgtactg
      181 tctcctagca gtttgcggaa aaaaggcctg aaacaggtta cgaccggaat gttctaagcg
      241 tctcttatgc atgcgcggaa tcgatcacca gcaagccgac atgtacagct acatttcgcc
      301 cgaggcgcga gtgagagggg accatccgct gcgaaagatt cgaactatgg cggatgaagc
      361 cttgaacaac atgtcggcac ggtttgatgc gatgtacgcc aagatcggac gcccatcgat
      421 cccaccagag aaactgctgc gcgcgcaatt ggtccaaatg ctgtattcga tccgcagcga
      481 acggttgttg atggaggaga ttgactacag catgctgttc cggtggtttg tgggaatgaa
      541 cttggacgag ccggtgtggg atgcgacggt gttcacgaag aatcggaacc ggctgctgga
      601 aggcgacgtg gctcgtgaat ttctctgtga ggtggttaag caagctcaat ccaacgggct
      661 gaccagtgat gaacatttca cggtagacgg cacgttgatc gaagcttggg caagtctgaa
      721 gagcttccag cggaaggacc aaaacaacca accgccggac gatcctggaa atccgaccgt
      781 gaactttcac ggggagaagc gaagcaacca gactcacgaa tccaccacgg atgccgacgc
      841 gctgttggcc cgtaagggca acggcaagga ggccaagttg agctacaacg ggaacctgct
      901 gaccgagaat cgtaatggac tgatcgtaaa cacggaagtg tttcccgcca acggcatggc
      961 ggagcgagcc gcggccatgg cgatgttgga acaagttcgg ggcgaccatc gagttacagt
     1021 gggggccgac aaaggatacg acacgcggga cttcgtggcc gagtgccggc atctgcacgt
     1081 caccccgcac gtggcgcaga acacgaaacg cagcgggggc agcgctattg acgcccgaac
     1141 gacacggcat ggcggttacg cggtcagcca gaagaaacgc aaacgagtcg aagagtgttt
     1201 cggttggatg aagac
//
LOCUS       DASTWE010000949         1296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_122531, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000949 DASTWE010000000
VERSION     DASTWE010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122531"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFT30_09905"
     CDS             <1..336
                     /locus_tag="VFT30_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5092990.1"
                     /translation="LLKPFGYTKEMDELMTASDLVLGKPGGLTTSEALAKGLVFVIVN
                     PIPGQEERNSDHLLESAAAIRCNNLPTLSYKLDQLLADPARFKSMQANSRRMGHPNAA
                     MQIVEQLLK"
     gene            complement(449..>1296)
                     /locus_tag="VFT30_09910"
     CDS             complement(449..>1296)
                     /locus_tag="VFT30_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5092991.1"
                     /translation="KAEGGVISVFFVDDNFAISIKRTKSLLRDIIAAGAQLPWIAQIS
                     ANLLRDQELVDLIAESGGKVIFIGMESIDPVNLADVKKGFNKPEEYTGVLRRLAAHNI
                     YAITSFIIGMDNDTPGVADRILEQLRTWPPGLPVFGQLTPFPSTPLYQRLQADGRLVR
                     PKHWLKFEKNTMAHIPLNMTIEETHRELHHAWARSYDAARNAEAVQAMSDAPVGPRIF
                     HFVTRLAFRAIYFPQTTRRAWIKVFFENRRTVFSLGKEAIGTVLTPKKRMKEKVAIPL
                     SVDPG"
BASE COUNT          306 a          339 c          354 g          297 t
ORIGIN      
        1 cttttgaaac ccttcggcta cacgaaagag atggacgagc tgatgacggc gtcggatctt
       61 gtgctcggca aaccgggcgg gctgacgact tccgaagcgc tggcaaaagg actggtattc
      121 gtaatcgtta atccgattcc cgggcaggaa gaacgtaatt cggatcatct cctcgagagt
      181 gccgccgcga tccgctgcaa caatctgccc acgttgtcct ataaactcga tcaactgtta
      241 gcggatccgg cgcgcttcaa gtcgatgcaa gccaactcac gacgaatggg acatcccaac
      301 gccgcgatgc aaatcgtcga acaattattg aaatgatgcg cagatggatg gggtcagtac
      361 cgggcgcggt agcgccgggc taatctcaga tgggagcgcc gtgattggac ggtcaagccg
      421 gcgctaccgc gctcggttcc gacccgtatc aaccggggtc taccgaaaga ggaatcgcca
      481 ctttttcctt cattcgtttc ttcggagtta acacagttcc gatcgcttct tttccaagac
      541 taaacaccgt gcgcctgttc tcaaaaaata ctttgatcca agctcgtctg gttgtttgcg
      601 gaaaatagat agcgcggaag gcaagccgag taacgaagtg aaaaattcgc ggaccgaccg
      661 gtgcatcgct catcgcctga accgcttctg cgttacgcgc agcatcgtat gagcgcgccc
      721 aggcatgatg gagctcacgg tgagtttcct cgatggtcat gttgagaggt atgtgagcca
      781 tcgtgttttt ctcaaacttc agccagtgct ttggacgaac gagtcgacca tcagcttgta
      841 accgctgata aagcggtgtg gacggaaagg gtgtgagctg gccgaagacc ggcaagcccg
      901 gaggccacgt gcggagctgt tccaatatcc ggtcagccac gcccggtgtg tcgttatcca
      961 tgccaatgat gaaggaagtg atggcgtaga tattgtgtgc agccagacgc ctcagcacgc
     1021 ctgtgtattc ttcaggcttg ttgaatccct tcttaacgtc ggccagatta accgggtcaa
     1081 tcgactccat cccgataaag atgaccttcc caccggattc agcaattaga tcaaccagtt
     1141 cctgatcacg taatagattg gcgctgatct gcgcgatcca tggtagttgc gcaccagcag
     1201 cgatgatatc gcgcagtaga gatttggttc gtttgatact gatggcaaag ttgtcgtcaa
     1261 cgaaaaagac cgagattact ccgccttcgg ctttag
//
LOCUS       DASTWE010000950         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1609435, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000950 DASTWE010000000
VERSION     DASTWE010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609435"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFT30_09915"
     CDS             complement(<1..891)
                     /locus_tag="VFT30_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEU5092992.1"
                     /translation="MDQRIAFLEAENAALHTRLDSLGTDSGNAVVLDDERVLQDVGIY
                     RYHHPLENALAYRERLDDIAARIAETIKAGAAIEKSNMFTFDGSLAKGRAMTNNLAKL
                     MLRAYNAEAENVVRSLHAGNTVTAIRRLDAARTAIVKLGKMMEMHIKEEFHALRIKEI
                     ELTADYLMKKEEEREAARAERERLREERKVELELAASRERLEKERSHILTVIEKVRAG
                     GSNDPELERKLADVDSAIAQNDYRAANIRAGYVYVISNRGAFGDHIVKIGLTRRLEPL
                     ERIYELGDASVPFRFDVHAIF"
BASE COUNT          337 a          490 c          439 g          391 t
ORIGIN      
        1 gaaaattgca tgcacgtcga aacggaatgg gaccgacgcg tctccaagtt cgtagatgcg
       61 ctcgagcggt tctagccgac gagttagccc aatcttcaca atgtgatcac cgaacgcgcc
      121 gcggttcgag atgacataga catagcccgc ccgaatgttg gcagcgcggt agtcgttctg
      181 agcaattgca ctgtccacgt cagcaagctt gcgctcaagt tcagggtcat tcgaaccacc
      241 cgcccggacc ttttcaatca ccgtcaggat gtggcttcgc tccttctcaa gacgctctcg
      301 agaggcagcg agttctagct cgaccttacg ctcctcccgt agccgttccc gctcagcacg
      361 cgccgcttct cgctcttcct ctttcttcat caagtaatcg gccgtgagct caatttcttt
      421 gatgcgtaag gcatggaact cttctttgat atgcatctcc atcatcttgc cgagctttac
      481 gatcgccgtt ctggccgcgt caagacgcct gatggcggtc acggtgtttc ctgcgtgaag
      541 cgaccgcacc acgttctcag cttcagcatt gtaggcgcgg agcatcagct tggccaagtt
      601 gttcgtcatc gcgcggccct tcgccaacga gccgtcgaag gtgaacatgt ttgacttttc
      661 gattgcggcg cccgccttaa tcgtctcagc gatccgagcc gctatgtcat cgagtcgttc
      721 ccgataggca agcgcgtttt caagggggtg gtgataccga tagatcccga cgtcctgaag
      781 aacacgttcg tcgtcgagga cgacggcgtt tcctgagtcc gtaccaaggg agtccaagcg
      841 tgtgtgcaag gccgcgttct ccgcctccaa gaacgcgatc cgctggtcca tactcgttag
      901 aaaatgcgtc ttagattcgc cgtgaggctt ctccgtagcg tcagagggat ctgatccgct
      961 gccgaatata ctatctcccg ttggggtacc gtctggaatc caaagctgcc aaccagggtg
     1021 gtggagcagg ccaagcaggg tctggtttcc agccatttgg gggcgtccac cccttgggag
     1081 gcttaggcca cccaggcggc ggattgaaga ccaacgggct gcgctgcgtc acgaccgacg
     1141 gactccggag cgctcggcag cgaccaggct atatgggttc ttcgaaatga cagcgccgag
     1201 ccgtcccagc gtgagtgcag gtacaaccgc cgcggcatcc gttcctattg gaccgagaca
     1261 tattgtgatg cgcggcgcat tttcactcga tccagcaagt cagtcaagtc tcacttaccc
     1321 aaaagtcctg aggatcgctt ctggtcttta aagcatggag aattgaggag gtactggtct
     1381 ctttgagagc aagcggatta cgatcattgc ggcttatttc tacccttctg atatactccg
     1441 ctatgctccg cttctatcga tccgatccga cacctcgaat gccacggcgt tgagttccga
     1501 aaccataacg ccgtttgact ccccttccct cgtttgttac tctccgcgcg aattgaggag
     1561 tactcaggaa aacagactat atgaacatcg cggagtatct cgatcgaaaa cggatcgacg
     1621 tcgatcgatt tctcgacctc gtcgcgccac cctctat
//
LOCUS       DASTWE010000951         1958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_672105, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000951 DASTWE010000000
VERSION     DASTWE010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672105"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFT30_09920"
     CDS             <1..894
                     /locus_tag="VFT30_09920"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU5092993.1"
                     /translation="LPLGIKDTGVGAIRSTLQIGEPAVIAERTGITTVANFRPRDIAA
                     GGQGAPLTPGVHALLFRHPRRARLIVNLGGISNVTYVPPASGSAELVAFDTGPANMVL
                     DGLMSRTTNGRVSMDREGRLAAKGRVDSRLLAKLLAHPYLSQAPPKSTGREAFGAKML
                     DELLNWQQTRRLPIEDLLATCSRWTAEAVGTARRWIKGGIDDVIVGGGGVRNRAIMGH
                     LTDIFAPVPVTPFESHGWDSKALESVAFAVLAYQTVMEQCGNVPSVTGAMSPRLLGCI
                     VPSGPHWYERLRSRKGGSKKR"
     gene            891..1394
                     /locus_tag="VFT30_09925"
     CDS             891..1394
                     /locus_tag="VFT30_09925"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HEU5092994.1"
                     /translation="MKILLVGAVIAVVIMLWHYLRSARRRKKQTSPFAIPPGVTIGST
                     RLLEKEEASLYSLLQMAVQERYLVFSQVPLWSFVEIEAGAKVRSDVLRQIALKRVEFA
                     LVHPGSCCVEQVVQIEDESQSYQAERQHVIKSVLDAAGIKLTKLTSKESYSLPDLAAR
                     LGITAEE"
     gene            complement(1430..>1958)
                     /gene="ybgF"
                     /locus_tag="VFT30_09930"
     CDS             complement(1430..>1958)
                     /gene="ybgF"
                     /locus_tag="VFT30_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU5092995.1"
                     /translation="NPFDWPYEKVEAVMLRKCIGILILFIFFSVDQSLAAPTQRQDTT
                     RQLYDRVMDEFKHRDYEAAMAGFRLFIELHSQSALAANAQYWIGECQYRMRRYRDALK
                     SFYEVVSNYPLSPKLAASTLKLGQTYTKLGDHEKARLMFDRVVEQYPDSSEAEVARKI
                     IEASPSAEGSTNQSP"
BASE COUNT          420 a          512 c          579 g          447 t
ORIGIN      
        1 ctgccgcttg gcatcaaaga tacaggtgtc ggtgcaatac gctccacatt gcaaatcggt
       61 gagcccgctg tgattgccga acgcacgggg attaccacgg tggccaattt ccgtccacgc
      121 gacatcgcgg ctggcgggca aggggcgccg cttacaccgg gggtccatgc gctgctgttt
      181 cggcatccgc gccgcgcacg gctcatcgtg aatcttggcg gcatcagcaa cgtcacttac
      241 gttcctccgg catccggctc tgcggaactc gtcgcgttcg acaccggacc ggcaaatatg
      301 gttctcgatg ggctcatgtc tcgcaccacc aatggtcggg tatccatgga ccgtgagggg
      361 cgcttggcag ccaaaggccg ggtcgattca agattactcg ccaaacttct agcacatccg
      421 tatctttctc aggcgccccc caaatcgact ggtcgtgaag cgttcggcgc gaagatgctg
      481 gatgaattac tcaattggca acaaacacgt cggttaccga tcgaagatct tctggccacc
      541 tgtagccgct ggactgccga agcggtcggt acggcacggc ggtggatcaa gggaggaatc
      601 gatgacgtga tcgtgggcgg gggtggtgtc agaaaccggg cgatcatggg acatttaacc
      661 gacatctttg ctccagtgcc ggtcacgccg tttgagtcgc acggttggga cagtaaggct
      721 ctggaatcgg tggcttttgc cgtcctggcg tatcagaccg tgatggaaca atgcgggaat
      781 gtcccatcgg ttacgggggc tatgtcccca agattgttgg gttgcatcgt cccaagcggc
      841 ccgcactggt atgagcggtt gcgatcgcgc aaaggcggaa gtaagaagag atgaaaattc
      901 ttttggttgg tgcggtcatc gcagtggtca ttatgctttg gcactatctg aggagcgcac
      961 gccgaagaaa gaagcagaca tcgccgtttg ccattcctcc cggcgtcacc atcggttcta
     1021 cccgattgct cgagaaagag gaggcttcgt tgtatagcct cctgcaaatg gcggttcagg
     1081 agcgctacct tgtgtttagt caagtgccac tgtggtcctt cgtggagatc gaagcagggg
     1141 caaaggtgcg ttccgatgtt ttgcgtcaga tcgcactcaa acgagtcgag tttgctttag
     1201 tccatcctgg gtcgtgctgc gtcgaacagg tcgtgcagat cgaggatgaa tcgcaatctt
     1261 atcaggctga acggcagcac gtcatcaagt cggtgctcga cgcggcggga atcaagctga
     1321 cgaagttgac gtcaaaggaa agctattcac tgcctgatct cgccgctcga ctggggatta
     1381 ccgcagagga atgacgaaag acgagtcgcg tcaatgaaca cgtgagtgcc tacggcgatt
     1441 gattcgtcga gccttcggca cttgggctgg cctcgatgat tttgcgggcg acttctgctt
     1501 ccgagctatc ggggtattgc tccaccaccc ggtcgaacat aagtcgtgcc ttctcgtgat
     1561 cacccagctt cgtgtacgtt tggccgagct tcagcgtgga agccgccaat ttggggctaa
     1621 gcgggtaatt ggacacgacc tcatagaacg acttgagcgc gtctcgatat cgtctcattc
     1681 gatattgaca ctctccgatc caatattgcg cattggccgc taaggcggat tggctatgca
     1741 gctcgatgaa gagtcggaaa cctgccatag ctgcctcgta gtcacggtgc ttaaactcat
     1801 ccatgacgcg atcgtataat tggcgggttg tatcctgacg ctgagtaggg gcagccaagg
     1861 actgatcgac cgagaagaaa ataaatagga tgagaattcc gatacatttt ctgagcataa
     1921 cggcctccac tttctcatat ggccagtcga aaggattt
//
LOCUS       DASTWE010000952         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1383078, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000952 DASTWE010000000
VERSION     DASTWE010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383078"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="thiI"
                     /locus_tag="VFT30_09935"
     CDS             complement(<1..903)
                     /gene="thiI"
                     /locus_tag="VFT30_09935"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEU5092996.1"
                     /translation="MRCVIVHYHELALKGHNRDYFEQCLIKNIRTALNGVGVRQVENL
                     HSRIRIRLPHEASPEVVRDRLIRVCGIANFLPGRVVPLQLANPNLHELTAAVLGEIES
                     RSFATFRITARRADKRLALTSMDIEKSLGAAVCDRTGKKVSLKNPDLTIYIELLSKEA
                     FCSAEKIEGPGGMPVGVSGRIACLISGGIDSPVAAYRMVKRGCLASFIHFSGRPLVSR
                     ASEEKVHELVRHLTTFQYDSRLYVIPFGEIQREIILSAPAPFRIVLYRRMMIRIAQEL
                     ARKERCWTLVTGDSLGQVASQTPQN"
     gene            complement(969..>1340)
                     /locus_tag="VFT30_09940"
     CDS             complement(969..>1340)
                     /locus_tag="VFT30_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5092997.1"
                     /translation="DRAMKNEKIAFVWNSVVEDVLGQDVVTGVRIRNVITGKVWDLPC
                     AGFFLAIGHRPNTALFTGQLKMNGAGYLLTTHGTATNIPGVFAAGDVQDSHYRQAITA
                     AGTGCMAAIDAEHFLETSGQG"
BASE COUNT          300 a          362 c          371 g          307 t
ORIGIN      
        1 gttctgaggt gtttgagagg ccacttgacc cagactatcg ccagtcacta aggtccaaca
       61 ccgctctttt cttgccaact cctgggcgat tcggatcatc atccgtctgt acagtacgat
      121 gcggaacggg gcaggcgcgc tcaggatgat ctctcgttgg atttccccaa aaggaatcac
      181 atagagccgt gaatcgtatt gaaaggtggt gaggtgccgt acaagttcgt gaactttttc
      241 ctccgatgcc cggctgacca acggccgccc cgaaaagtga atgaaagaag caaggcagcc
      301 ccgctttacc atacgataag cggccacagg tgagtcgatc cctcccgaaa tcagacaggc
      361 aatccttcca ctcacgccga ccggcatgcc gccgggacct tcgatctttt ccgccgaaca
      421 aaaggcttcc ttcgacagga gttcgatata aatggtcaaa tcgggattct tcaaactcac
      481 tttctttccg gttctgtcgc agacagccgc accgagcgat ttttctatgt ccatcgaagt
      541 caacgccagg cgcttgtcgg cccgtctggc tgtgattctg aatgtagcga aggatcgcga
      601 ttcaatctct ccgaggacgg ccgctgtgag ctcatgcagg ttgggattag ccaattggag
      661 tggaaccaca cggcccggca aaaagttggc gatcccgcat acacgtatca gccggtctcg
      721 aaccacttcg ggggaggctt cgtgcgggag ccgaatgcgg attcgactgt gcaggttctc
      781 gacctgccgg actccgacac cattgagggc ggtccgaatg ttcttaatga ggcattgctc
      841 gaagtagtct cgattgtgcc ccttaagagc aagctcgtga taatggacga tgacacatcg
      901 catggtggag atagggtgag gtcgaggtta aggtgtaagc cttctttcgt gagcgctaag
      961 ccttaatctc aaccttggcc tgaagtttct aagaagtgct cggcgtcaat cgcagccatg
     1021 cagccggtac cggctgcggt aatggcctgg cgataatggg agtcttgcac atccccggcc
     1081 gcaaatacac cggggatatt ggtggctgtt ccgtgagtgg tcaggagata gcccgcgccg
     1141 ttcatcttca gttggccggt gaagagtgcg gtgtttgggc gatggccgat cgccagaaaa
     1201 aacccggcac agggcaaatc ccagactttt ccggtgatga cgtttctaat gcggacaccg
     1261 gtcacgacat cctgtccaag aacatcttcg acgacgctgt tccagacaaa ggcaattttt
     1321 tcattcttca tggcccggtc
//
LOCUS       DASTWE010000953         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1576951, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000953 DASTWE010000000
VERSION     DASTWE010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576951"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1136
                     /locus_tag="VFT30_09945"
     CDS             <1..>1136
                     /locus_tag="VFT30_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5092998.1"
                     /translation="IGAGPAGQKAAVQGAKAGKRVALIERERGIGGSCVYRGTIPSKT
                     LRESALHLDRLKRAGEAFQFSLKPDTQIATLLSRLEDVVQAHDSFMSRQIRRNGISLF
                     HGRAHFLSEHMVEMQTVDGAQQQFTADTIVVATGSRPRNPEEIPVDHEHILDSDSLLS
                     MMYLPRSLAVVGGGVIGCEYASIFALLGVEVTLIDRASYPLQFMDRELVEQFIKSFEQ
                     QGGHYLGGQHIEKVQWNEVAHVVTKLSDGTTINTEKMLVALGRQANVEDLNLSAAGIM
                     VSDKGVIPVNQYCQTNVSHVYVVGDMVGAPALASKAMEHGRRAVRHALNLPIGDAAST
                     IPLGIYTIPEMASIGLDEMGARERYRDPLIGRAKFEEIARAQISG"
BASE COUNT          259 a          312 c          331 g          234 t
ORIGIN      
        1 atcggtgccg gtccagccgg ccaaaaagcg gcggtccaag gtgctaaggc cggtaagcgc
       61 gtcgcgttga ttgagcgaga gcgcggcatc ggaggcagct gtgtctatcg cggcacgatc
      121 cccagcaaaa cgttgcgaga aagcgccctc catctggatc gactcaaacg ggcaggagaa
      181 gcgttccaat tcagcctcaa gccagatact caaattgcaa ccctcttaag ccgtcttgag
      241 gacgtcgtcc aagcacacga ctcctttatg agccgtcaga tcagacgcaa cggtatctcg
      301 ctgtttcacg gacgagcgca tttccttagt gagcacatgg tcgagatgca aaccgtggac
      361 ggagcgcaac aacaattcac cgccgacacc atcgtggtag ccacgggctc gcgtccacga
      421 aatcccgagg agattcccgt cgaccatgag cacatccttg acagcgactc actgctttcc
      481 atgatgtact taccacggtc actggctgtc gtgggaggtg gtgtgatcgg ctgcgagtat
      541 gcttccatct ttgcgctatt gggagtagaa gtcaccctga tcgatcgcgc ttcctatccg
      601 cttcaattta tggatcgaga actggtcgaa cagttcatta aaagctttga acagcagggc
      661 ggccattatc tcggcggaca gcatatcgag aaggtgcaat ggaacgaggt cgcgcacgtc
      721 gtcacgaagc tgagcgatgg aacgacgatc aatactgaga agatgctggt cgcgctgggg
      781 cggcaggcca acgtcgaaga cctgaatctc tccgctgcgg ggatcatggt ttctgataaa
      841 ggtgtgatcc cggtgaatca atactgtcag acgaatgttt ctcacgttta tgtcgttggc
      901 gacatggtcg gtgcgccggc attggcgtcc aaagccatgg agcacggtcg acgcgccgtc
      961 cgccatgccc tcaatctccc gatcggtgat gcggcctcga ccatcccatt aggcatctat
     1021 acgatcccgg aaatggccag tatcgggctt gatgagatgg gcgcccggga gcgctatcga
     1081 gatccgttga tcggacgggc gaagtttgag gaaatcgcac gcgcccagat atcggg
//
LOCUS       DASTWE010000954         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_801252, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000954 DASTWE010000000
VERSION     DASTWE010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801252"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1025)
                     /locus_tag="VFT30_09950"
     CDS             complement(<1..>1025)
                     /locus_tag="VFT30_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU5092999.1"
                     /translation="LAFYQTAHQGQSRTPDLAVPYRDYIAWLQQQDAAAAETFWRTYL
                     EGVTAPTQLPNCTQSQPAGSSYGEHHVELSSDVTRALTNLAQAERVTLNTIVQAAWGM
                     VLAHFGGTDEVVFGITVAGRPHDLVGVESIMGVFINTLPLRVRLSPETKLTNWLREIQ
                     SSNIELRQYEHTPLVQVQSWSGMDRRTPLFETIVVFQNYPLDDAVEDYQQSLGIDVIR
                     AEGSTNYAITIDAIPGTQLRLTFSYDKQRIDDSTMHLMADHVVLLMEGMAAYPEARLA
                     DLPILTPAERTQLLIEWNATETKPFDDRCISEIFEAQVERTPDVIAVGYRNQRVSYRA
                     LNHRANR"
BASE COUNT          221 a          302 c          267 g          235 t
ORIGIN      
        1 tctgttcgcc cgatgattca aggcccggta actcacgcgc tggtttcgat acccgacggc
       61 gatgacatct ggtgtccgtt ccacctgcgc ctcgaagatt tctgagatgc atcgatcgtc
      121 gaaaggcttt gtctctgtag cgttccattc aatcaaaagc tgggtgcgtt ccgccggagt
      181 caatatcggc agatcagcga ggcgggcctc gggatatgct gccatgccct ccattagcaa
      241 aactacatgg tccgccatca gatgcatggt cgagtcatcg atacgctgtt tgtcatagga
      301 aaacgtcaga cgcagttgtg ttccgggtat cgcatcaatc gtgatcgcat agttcgtcga
      361 cccttcagct cgaatgacat cgatgcccaa cgactgctga tagtcttcca ccgcatcgtc
      421 cagcgggtaa ttttgaaaga ccacaatggt ctcgaataat ggcgttcgac gatccatccc
      481 gctccaactt tgcacctgta ccaaaggagt atgttcgtac tggcgcagct ctatgttcga
      541 ggactggatc tctcgcagcc agttcgtcaa cttggtctca ggcgagagac gcacgcgaag
      601 cggcagggta ttgatgaaga ctcccatgat cgattccacg ccgactaggt catgagggcg
      661 accggctacc gtgatcccaa acaccacttc gtccgtcccg ccaaagtgcg ccaacaccat
      721 tccccatgcc gcttgcacga tggtgtttaa ggtcacacgc tcggcctggg ccagattcgt
      781 taatgcccgt gtgacgtcgc tgctcaattc cacatggtgc tctccgtaag atgagccagc
      841 cggttgactc tgcgtacaat tcggcaactg cgtcggcgcc gtcactcctt ccaagtaggt
      901 ccgccaaaac gtctcggccg cagccgcatc ctgttgctga agccatgcga tatagtcacg
      961 atagggaacc gcgagatcgg gcgtccgact ctgcccttga tgggccgtct gatagaaagc
     1021 cagaa
//
LOCUS       DASTWE010000955         1163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1157066, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000955 DASTWE010000000
VERSION     DASTWE010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157066"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..933)
                     /locus_tag="VFT30_09955"
     CDS             complement(208..933)
                     /locus_tag="VFT30_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5093000.1"
                     /translation="MDIAQVTIGLAFLAGLASFLSPCVFSLVPAYIGYLGGRAAGGEA
                     TENNRFITFTHGLAFVLGFSAVFITLGVASSAFGRLLFDLRFILAKVGGIVVIIFGLH
                     MIGVFRIPFLEYDTRVQQMPDRKWGYLSSALMGVFFSAGWAPCVGPVLGAILTLALNG
                     GSVSTGVSLLSAYSAGLAIPFLVAAMGVGWVSLTLRKYGKVMHYIEIAMGVILVILGV
                     MLFAGVFERIAQAGQFFWIDFGI"
     gene            complement(951..>1163)
                     /locus_tag="VFT30_09960"
     CDS             complement(951..>1163)
                     /locus_tag="VFT30_09960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU5093001.1"
                     /translation="MISGDGVAIHGAFWHNAFGEKRSHGCINVTPEDAKWIFRWTTPH
                     VSLAQSEMRVSLPDHGTIVVSTETKV"
BASE COUNT          316 a          329 c          305 g          213 t
ORIGIN      
        1 agctttgagt gcatacggac ccacttcaac aacagggatc tccgccaggt attttttctg
       61 cagggcgggg tccgtatcga tatcgatctc tacgagacgg tgcgggtatt tctcctggat
      121 ggaatccaga tcggctttgg cctgttcaca taaatgacag tcctggcgca tgtaaagggt
      181 aacggtcagc atgagaatga atacagacta aataccgaaa tcgatccaga agaattgacc
      241 ggcttgcgcg atgcgctcga aaactcccgc gaagagcatc acgcccagga tgaccaaaat
      301 cacgcccatg gcgatctcaa tatagtgcat caccttgcca tattttcgca aagtgagcga
      361 gacccaaccc acccccatag ccgcgaccag gaacgggatc gccagcccgg cggaataggc
      421 agagagcagt gaaacgccgg tggatacaga gccaccatta agagcaagtg tcaggatcgc
      481 gccgagaacc ggtcccacac acggcgccca accggcggag aagaacacac ccattaacgc
      541 agaagaaagg tagccccatt tacgatccgg catctgctgc acgcgcgtgt cgtactcaag
      601 gaacgggata cggaagacgc caatcatgtg caagccaaaa atgatcacaa cgatcccgcc
      661 gacttttgca agaataaagc gtagatcgaa gagcagacga ccaaaggcgg atgacgcaac
      721 cccaagcgtg atgaataccg cgctgaaccc gagcacaaat gccagtccat gtgtaaacgt
      781 gatgaagcga ttgttctcgg tcgcctcacc ccccgcggca cgcccaccca ggtagccgat
      841 atacgcgggc acaagagaga acacacacgg cgaaaggaag gatgccaatc ccgcgagaaa
      901 cgcaagtccg atggttacct gtgcgatgtc catgctttac cttttgaatt ctatactttt
      961 gtctcggtgc tcaccacaat ggtgccgtga tccggaagag atacgcgcat ctcactttga
     1021 gcaagagaca catggggcgt cgtccagcgg aagatccatt ttgcgtcctc gggtgtgacg
     1081 ttgatgcagc catgcgagcg tttctccccg aaggcgttat gccagaacgc gccatggatc
     1141 gcgactccat caccgctgat cat
//
LOCUS       DASTWE010000956         1216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_413356, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000956 DASTWE010000000
VERSION     DASTWE010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413356"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..265)
                     /locus_tag="VFT30_09965"
     CDS             complement(143..265)
                     /locus_tag="VFT30_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093002.1"
                     /translation="MSGCSADELQKAKLIGARQIFQIPIHFHAVLHAVQYELLH"
     gene            complement(551..793)
                     /locus_tag="VFT30_09970"
     CDS             complement(551..793)
                     /locus_tag="VFT30_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093003.1"
                     /translation="MTCTRCQGLLLEEHMIDMEAGYGEMWSRGWRCVNCGHRDDAVVQ
                     HHRQLHTRQMVNPQAVTVQKSVGLSWASESVEPLGA"
BASE COUNT          303 a          332 c          310 g          271 t
ORIGIN      
        1 ttgaaacccc tgttcatagg tcattgggcc gatgaccgga aagcgacgat aaggtgtaaa
       61 acaaaggcca tcatcatctc gacagggtgg tgccgagaat tcgcgggtct atgtcaacca
      121 tcgcctggat tcgaggtcgc agctagtgca gcaactcata ctgaaccgcg tggagcaccg
      181 catggaaatg aatcggtatc tgaaagatct gccgagcccc gatgagcttc gccttctgaa
      241 gttcgtcggc ggagcatccg ctcatggcac aatgaccttc acgtctaaga acgcccgggt
      301 caattccaag atgacctcca ggccattcat ttcaggcatc tccagatccg taatgaccag
      361 attcacgggc tgggtgcgaa accgttcgag tccctgccgg ccattggcgg cttcatagac
      421 ctcataccca catcttcaag tacctgtgcc atcagcgtgc gtagggaaag catgatcctc
      481 aatgatcaga atgctggctg acctcatcac ccgcccttct agagatcccg ctcacgagtc
      541 gcccatctct ttacgctcca agaggttcca ccgactccga tgcccaggat aagccaacgg
      601 acttctgaac cgtcacggcc tgaggattca ccatttgcct ggtgtgtagc tgccggtggt
      661 gctgcacgac agcatcatct cgatggccac agttgacaca gcgccagccg cggctccaca
      721 tctctccata gcccgcttcc atatcgatca tatgttcttc cagcaacagg ccctgacagc
      781 gtgtacaggt catcgcatcc tccatgtggt gatcgcattg tgtctgagat aaccgtatca
      841 gggatgccga ttagagctag atccgaacga ggtacgtcca aaggggggaa gaggggcgag
      901 aatcagccta tcttctagta acctcacgcc gtcgaagatc gccggaccta aagggacatg
      961 aggcgtgaaa tacttgcgcc atgtgcatcg atgagaaagg cctaccttaa gtacaagggg
     1021 aatcactagt ggggtcataa ccaatatgag gcgatgataa cgactacaga tttacgacta
     1081 cagaatgtaa cgatggaaac cggtcactat caatcaggag gcatcatgat ctctcgttca
     1141 aaaggtgttc aaactgacag agtatttttg ctgggactag tcatcttgtt acaggctggt
     1201 tgtgcatcgt ctgctg
//
LOCUS       DASTWE010000957         1454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_963124, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000957 DASTWE010000000
VERSION     DASTWE010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963124"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFT30_09975"
     CDS             <1..1194
                     /locus_tag="VFT30_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEU5093004.1"
                     /translation="IIHTCGSTPSQGGRPVCSLGIRAGYMLIRNFAGIQGLGLKAGRQ
                     YIVFGDQSLFGHFDWANTGFSFDGVMVQHSHSIMDTHAGWFRTAETDLLQGAPLGSGN
                     PNIGGTGQARNAAGDADLFIFYNQIKTIPGFLIEPFYVYYKSNLGGGDISSQGLGTPK
                     HGNQTRHTIGGRVAMKKGYFDASSEVVYQFGQMGDTAAGASGLCGDPGGEGKCLHINA
                     WATRNWFGYTAFNLPGKPRIAFNFDYASGDGRANCTLDAAYGGCKTANTFENFFPTNH
                     IHMGYMDVIAWKNMMSFSGNFQARPTKDDHIEVWYTNLHLANARDNWYRASQGVYVFS
                     RPDNTETHIGDELDVVWTHFFMNGIVAFQAGYGHLWPGSYISHNLGRRAVGQDWAYAQ
                     LWINF"
BASE COUNT          339 a          390 c          369 g          356 t
ORIGIN      
        1 attatccata catgcggttc tacgccaagt cagggtggaa gacccgtctg ttcactgggt
       61 atacgcgccg gttacatgtt gatccggaac tttgccggta tacaaggctt aggtctcaag
      121 gccggtcgac agtatatcgt cttcggcgac caaagcctgt tcggccactt tgattgggcc
      181 aacacaggct tctcatttga cggcgtcatg gtgcagcaca gccacagcat catggatacc
      241 catgccggct ggtttcgaac agcagaaacc gaccttttac aaggagcgcc cctcggcagc
      301 ggaaacccga atatcggtgg gacaggtcag gcgagaaacg ccgccggtga tgccgatttg
      361 ttcatctttt ataaccaaat caaaacaatc cctggatttc tcatcgaacc cttctatgtc
      421 tactacaaga gtaatcttgg ggggggagat atctcttctc aaggtctcgg caccccgaag
      481 cacggcaatc aaacccgcca taccatcgga ggacgggtgg ccatgaagaa aggctacttc
      541 gatgcctcga gcgaagtggt ctatcagttc gggcagatgg gggataccgc cgccggcgca
      601 agcggcctgt gcggggatcc tggaggcgag ggaaaatgtc tccacatcaa tgcttgggcc
      661 actagaaact ggtttgggta taccgccttc aacttaccgg gaaaaccccg catcgccttc
      721 aattttgact atgcgtctgg cgacggccgt gcaaattgta cgttagacgc cgcctatggt
      781 ggttgtaaga ccgccaatac gtttgaaaac tttttcccga cgaatcacat ccacatgggg
      841 tatatggatg tcatagcgtg gaaaaacatg atgagcttct ccggtaactt tcaagcgcgt
      901 ccgaccaaag acgatcacat tgaagtgtgg tatacgaacc tgcatcttgc caatgcccga
      961 gataattggt atcgcgcaag tcagggcgtc tatgtcttct cacggccgga caacaccgag
     1021 actcatatcg gtgacgaact cgatgtcgtt tggacccatt tttttatgaa cggcatcgtc
     1081 gcctttcaag ccggttatgg tcacctctgg cctggatcgt atatttccca caatctgggg
     1141 cgccgtgctg taggacaaga ttgggcctac gcccagctct ggataaattt ttaggctgct
     1201 ccatcgtcag agacagcaaa gcttcggtga tacagccttc atgtcctgga cgttgccacg
     1261 aaaccccaat gaacctgatc ctttgaaaag gcctattgtc ttacgatcga tcgctatctg
     1321 tacgattggt gcactcctct tctttgcttt gacttcgtcg gagtccaatc acaactcgtg
     1381 gatcgccggc tgaacctatc gctctatata gccgcttgaa ctagtcgctc ctgaagttcg
     1441 ccatagttct gcgt
//
LOCUS       DASTWE010000958         1975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1415885, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000958 DASTWE010000000
VERSION     DASTWE010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415885"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VFT30_09980"
     CDS             <1..1782
                     /locus_tag="VFT30_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5093005.1"
                     /translation="QYFLRGFDADHGTDVAFFLDGMPINLRSHAHGQGYTDLNFIIPE
                     TIEGVEVNKGAYLPEYGDFATAGTINFQTRQVVKEGMIQAAGGEFSTQRYMLMFSPTK
                     ERVRSLFSAEGYYTNGPYINDNQYVRSNVLGKITTYLTGRDELSVKGTFLYSKWDGSG
                     EIPLRAVTDGLLNRFGAIDPYEGGNTLRTTGQLDYHYDTTSGGQFYMKAYGQYYRLDL
                     FTNFTFFLNDPVNGDGFAQFDRRAIYGGDIGYKQRGKILGMPSIGTVGFQTRVDDVHA
                     KLGTQLRKNLTDTTIDSDAFSASYAPFVKAEVQPLPWIRFTGGVRGEVFTFDVSNRCV
                     VCVEQSAGQTSSGIVLPKMNMILGPWAGTEFFINYGEGFHSNDARSAVRPGSSPISRS
                     INYEIGVRSRPWGPQGLELLATLWRMDIKSELVFVGDEGTTEIRGATRRQGVEVAARG
                     QVWGPLYVNGSFTWTHAEFRNGDAVPLAPEYIAYGAAILKWPEGLTSQLQATYMGVRP
                     LIEDRSIKAPSWITFDLSERYLIPMRLPHGRMEVFLYVQNLFNTDWEQAIFAFESRLR
                     NEPVAVNDIHFVPGNPRTFLGGLAYFF"
BASE COUNT          466 a          507 c          555 g          447 t
ORIGIN      
        1 cagtattttc tgcgcggctt cgatgccgac catgggacgg atgtcgcgtt cttcttggac
       61 ggcatgccga tcaatctgcg ctcccatgcg catggacagg gctataccga tttgaatttc
      121 atcattcccg aaacaatcga aggggtcgaa gtgaataaag gcgcctatct acctgagtac
      181 ggagatttcg cgacagcggg aacgatcaat tttcagacgc gccaggtggt aaaggaagga
      241 atgattcaag ccgccggcgg agagttcagc acgcagcgat acatgctcat gttttctccg
      301 accaaagagc gggtgcgcag cctcttctct gccgaagggt attacaccaa tggcccgtat
      361 ataaacgaca accagtatgt tcgatccaat gtgctaggca aaatcacgac ttatctgacc
      421 gggcgggacg aactcagcgt caagggcacc tttctctatt ccaaatggga cggctccggc
      481 gagatcccgt tacgggctgt gacagacggt ttgctgaacc ggttcggtgc aatcgatccc
      541 tatgagggag gaaacacact gcgtacaacc ggccaattgg actaccacta cgacacgact
      601 tcaggcggcc agttctatat gaaggcgtat ggacaatatt accggctgga tctgttcacg
      661 aatttcacgt tttttctcaa cgatcccgtc aatggcgacg gctttgcgca attcgatcgc
      721 cgcgctatct atggtggcga catcggatat aaacagcgag gaaagattct ggggatgccg
      781 agcatcggaa ccgtggggtt ccaaaccaga gtggacgatg ttcatgcgaa actgggcacg
      841 cagttacgga agaacttgac cgacacgacc atcgacagcg acgcgttctc ggcctcctat
      901 gcgccgtttg ttaaagcaga agtgcagccg ctgccgtgga tcaggtttac aggcggcgta
      961 cgaggcgagg tgttcacatt cgacgtgagt aaccgctgtg tcgtttgcgt ggagcaatcg
     1021 gccggacaga caagctccgg catcgtcctc cccaaaatga acatgattct tggtccatgg
     1081 gcgggaacgg aattcttcat caactacggc gaaggctttc atagcaacga tgcgcggtcc
     1141 gccgtaaggc cagggtcttc tcccatatcc cgttccatca actatgaaat cggggtacga
     1201 tccaggcctt ggggaccgca agggctcgaa ttactcgcga ctctctggcg aatggatatc
     1261 aagtcggaac tcgtgttcgt cggcgatgaa ggcaccacgg agattcgagg cgcaaccaga
     1321 cgccaaggtg tggaagtcgc cgcgcgcggc caagtatggg ggccgctcta tgtcaacggc
     1381 agtttcacct ggacacacgc cgagtttcgc aacggtgacg ccgttccttt ggcaccggag
     1441 tacatcgctt acggagccgc tattctgaaa tggccggaag gattgacttc tcaacttcag
     1501 gctacatata tgggagttcg gcctcttatt gaggatcgca gcatcaaagc tccgtcgtgg
     1561 atcacgtttg atctttccga gcggtatctc attccgatga ggcttccgca tggccggatg
     1621 gaggtatttc tgtacgttca gaatctgttc aacacggatt gggaacaggc gatcttcgcg
     1681 ttcgaatcaa gactgaggaa tgagccggtg gcagtcaacg acattcattt cgtgccggga
     1741 aatcctcgaa cgttcttggg cggccttgcc tatttctttt aaggagatcg ctcaggagag
     1801 ttgtccaggg taaggggtgg ggttcaaagc cgccgagggg ttatgataaa tgacagtggt
     1861 ctctaaggta aggagtctca tatgaaaccg acctctattt cacaattcat cgaccatcat
     1921 tatcgacatt tcaatgcggc ggcgatgaaa gacgccgcgc acgcttataa ggctc
//
LOCUS       DASTWE010000959         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_219639, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000959 DASTWE010000000
VERSION     DASTWE010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219639"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="VFT30_09985"
     CDS             <1..1399
                     /locus_tag="VFT30_09985"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HEU5093006.1"
                     /translation="APESSISVPIHVDLSPFLAVANDDSVIPKKFDHWGSFIKHPKGA
                     EYKYYAERDNFAVAPTRLPQTNNTQSGLALSGWWKGIELSSSHVLVGTALRYKIASPS
                     LHCGDGSEWPRRATVQGSIAAELTPTYGLSASVASVAVSASDPCQIRVADLDVAQEVR
                     QRLADIVRGGLSRAVTRINTLTVKSEMEAAWNTLRNPIQLEPDAWLQFNIDKVGHSGF
                     SGVGPMVDDTIHITAKPVIVFGPEPPAGGTTLPPLDTPPTSTGFHGAADAQLYGTLPT
                     TLANRLTPTGFHVVTDIPLDYASFSKALANRLNGKRVVKKGYFLLITNAAIFGNGGNQ
                     VVMRIDFSGDAIGHVYFVGKPEMNFLTQTVSLGGLRYDLATEKLIQKTVAWLDFSTFR
                     DLIASESVMGVTAATDRVRGLLATTLNRALSPSVSMHGTVESVQGIGVFADVNALHIR
                     TMSDGTLGLTVTDKP"
     gene            complement(1464..>2378)
                     /locus_tag="VFT30_09990"
     CDS             complement(1464..>2378)
                     /locus_tag="VFT30_09990"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEU5093007.1"
                     /translation="EALASNIGGTATLVGDPPNIMIASKAELSYLDFLSVMGPIVIVI
                     MAVFLALLWILFGRTMRVAPHLRENILALSSREAVPDEAFLRRCLILLAVVNVGFCVH
                     SLVHLEPATIALLGASLFMVIGHARRNPEDAEELTYLAEVEWKTIFFFIGLFILVGGL
                     VKVGVIRYLADQLVTVTKGNLTGSTMAVLWGSAILSAVVDNIPYVAAMNPLIVDLARS
                     LHPEITDYVALVHQPDIIPLWWALALGACLGGNGTIIGASANVVIVDIARKAGYRITF
                     WQFLKFGVPVMIGSVALSAIYLWLVFLR"
BASE COUNT          584 a          700 c          602 g          492 t
ORIGIN      
        1 ggccccagaa tccagtatca gcgttcctat ccatgttgat ctgtcaccgt ttctcgctgt
       61 tgctaacgat gacagcgtga tcccgaagaa gttcgaccat tggggaagct tcatcaaaca
      121 tcctaaaggc gctgaataca aatactatgc agagcgagat aattttgcgg tggcacctac
      181 ccgtctcccc caaaccaaca acacacagtc aggcctggct ctcagtggat ggtggaaggg
      241 gattgaactt tccagctccc acgtacttgt cggcaccgct cttcgatata aaattgcatc
      301 cccttcgctt cactgtggcg atggcagtga atggccacga agggccacag tgcaagggag
      361 tatcgcggct gagctgactc cgacgtacgg gctgtccgca tcggtcgcca gcgtggccgt
      421 gagcgccagt gatccatgcc agatacgagt cgcggatctt gacgtggcac aggaagtgag
      481 gcagagactg gcagacatcg ttcgaggagg actcagccgc gcggtcaccc gcatcaatac
      541 actgacggtt aaatctgaaa tggaagctgc ctggaataca ctgcgcaacc ccatccagtt
      601 agaaccggat gcctggctcc agttcaatat cgacaaagtc ggacatagtg gcttttctgg
      661 ggtcggcccc atggtcgatg ataccatcca tatcactgcg aaaccggtca tcgtcttcgg
      721 tccggaacca cccgccggag gcacaaccct tcctccactc gacactccac caacttctac
      781 cggattccac ggtgctgctg atgcccagct ctatggcaca ctccccacaa cactggcgaa
      841 ccggctgact ccaacgggat tccacgtcgt cacagacatc cccttggact acgcctcatt
      901 ctccaaggcc cttgcgaacc ggctcaatgg aaaacgcgtc gttaagaaag ggtatttcct
      961 tctcattaca aatgccgcga tctttggtaa tggcggcaac caagtggtga tgcggattga
     1021 tttctcggga gacgcgatcg gtcacgtgta ttttgtcgga aaacctgaaa tgaacttcct
     1081 cacacagacg gtttctctcg gcggcctccg ctatgacctc gcgacagaaa agctcataca
     1141 gaaaacagtc gcttggctcg atttctcgac gttccgagat ctgatcgcaa gcgaatccgt
     1201 tatgggagta actgcagcca ccgaccgtgt gcgaggccta ctggccacca cactgaatcg
     1261 agcacttagt ccatccgtct cgatgcacgg aaccgtggag tcggtccaag gcatcggagt
     1321 gtttgctgat gtcaatgcat tgcatatccg cacgatgagt gatggaacgc ttggtctgac
     1381 cgtcaccgat aaaccgtaat tcatcttgat cgagcctcgc acctcggttc gttgggaatg
     1441 gctgcagatc ggacggaggc gacctatcgc aggaacacca gccagagata aatcgcgctg
     1501 agtgccaccg acccgatcat gaccggaacc ccgaacttca ggaactgcca aaaggtaatc
     1561 cgatagccgg ctttgcgtgc gatgtcgacg atcacgacat tggcgctcgc gccgatgatc
     1621 gtgccgttac cgcctaaaca agcgcccaat gccaatgccc accagagcgg gataatatcc
     1681 ggttgatgga ccaaggcgac gtagtcggtt atttccggat gtaacgatcg ggcaagatca
     1741 acgattaacg gattcatcgc cgcgacatag ggaatattgt ccactactgc agaaagtatc
     1801 gccgatcccc acaacaccgc catcgtggat ccagtgagat tgccctttgt caccgtcacg
     1861 agttgatcgg ctagataccg gatcacaccg actttgacca gtccccccac caggataaat
     1921 aatccgatga aaaagaaaat ggtcttccac tccacttctg ccagatacgt caattcttca
     1981 gcgtcctccg gatttcgtcg ggcatggccg atcaccatga acaggcttgc tcccaataac
     2041 gcaatcgtgg ccggttccag atgaacgagc gaatggacgc agaatcccac gttgacgacc
     2101 gccagcaaga tcagacagcg gcgcaagaag gcctcatccg gcaccgcttc tcgtgaactc
     2161 aaagcaagaa tgttttcccg cagatgcggc gccaccctca tggttcgtcc aaagaggatc
     2221 cacagcagcg ccagaaacac cgccataatg acaatcacga tcggccccat aacgctcagg
     2281 aagtcgagat agctgagttc cgctttgctg gcgatcatga tattaggtgg atctccgacg
     2341 agcgtagcgg tcccaccgat attggaagcc agcgcctc
//
LOCUS       DASTWE010000960         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1157252, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000960 DASTWE010000000
VERSION     DASTWE010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157252"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1542
                     /locus_tag="VFT30_09995"
     CDS             19..>1542
                     /locus_tag="VFT30_09995"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU5093008.1"
                     /translation="MKITEAEGTGFLETPLTLKAEGIYQKLPFTLSLDGGSYQNLRSS
                     KEPYPVKINLAAGKLKASVDGNLTEPLAMKGEDVTLDIQGDDMAKLYPLIRLVFPSTP
                     PYRLKGHLRHDGKVWSFANFSGRVGDSDLSGNIRVDTAPKRPFMKADLVSKLLDFDDL
                     AGFIGGKPGTGPGETASQEQKKQAAAKKDDRIFPDQRYDLERLRAMDADVRLRAKKIL
                     APKLPIDDLNASLKLTDGVLSFAPATFGVADGRIEIYSTFDGSKSPSKVKIDARLRHL
                     DLRRFLADTSFAQKTLSPVGGRIVLAGTGQSFRELMATASGNTFLAMSGGEISELLIR
                     LAGLDVANALGVAIRGDKPTPVRCALLNLQGQNGQMGVRTLVFDTANSVVSGEGNIDL
                     RDEKVNIVVLPVPKGFSPLSLRSYLRVSGGFKNISVFPDPIKTGTDSLFKKLFNVVTM
                     LVMTPFQPRDLGQGKDVDCDALIAQVQNQDPNGLVLKDVMKPSGEQMVSRKRNSAEQQ
                     "
BASE COUNT          381 a          420 c          414 g          327 t
ORIGIN      
        1 agcaaaaccc ggattgaaat gaagatcact gaagcagaag gcacgggatt tctagagacg
       61 ccgctcacgc tcaaggccga aggcatatat cagaaactgc cctttaccct ttcactggac
      121 ggcggttctt atcaaaatct acggagttca aaggagccat atcctgtgaa gatcaatctc
      181 gccgccggaa agctgaaagc cagcgtcgac ggtaatctaa ccgagccatt ggcgatgaaa
      241 ggggaagacg tcacgctcga tattcaaggc gacgacatgg ccaaactcta tccgctcatc
      301 cggctggtat ttccatcaac gccgccgtac cgtctcaaag gtcatttaag gcacgatggg
      361 aaagtctggt cattcgcaaa tttttccgga cgggtgggcg atagcgatct gtcaggaaac
      421 attcgtgtgg acactgcgcc gaagcgcccg ttcatgaagg ccgatctcgt ttccaagttg
      481 ctcgattttg acgacctggc cggtttcatc ggcggcaaac ctggaaccgg tcctggcgaa
      541 acggcgtcac aggaacaaaa aaagcaggct gccgctaaga aggacgatcg aatatttccg
      601 gatcaacgtt acgatctgga gcgtttgcga gccatggatg ccgacgttcg gctccgcgcg
      661 aaaaaaatcc tcgcgcccaa actacccatc gacgacctca acgctagtct gaaattgacc
      721 gacggagtgt tgagcttcgc gccggctact ttcggcgtcg ctgacggccg catagaaatc
      781 tacagcacgt tcgatggatc gaaaagtccc tcgaaagtga aaatcgacgc acggcttcgg
      841 catctcgatt tgagacgatt tctcgctgat acatccttcg cacaaaaaac cctcagccct
      901 gttggagggc gaatcgttct ggcggggacc ggccaatcct ttcgcgagct catggcgact
      961 gcctccggca ataccttctt ggccatgtcg ggcggagaaa ttagcgagtt gctgattcgc
     1021 ctggccggac tcgacgttgc caatgctttg ggcgtcgcca tccggggcga taagcccacc
     1081 cctgttcgct gtgccttatt aaatcttcag ggacagaatg gacagatggg ggttcgaaca
     1141 ctcgtcttcg ataccgccaa ttccgtcgtt tccggtgagg gcaatatcga cctccgagat
     1201 gagaaggtca acattgtggt gctgccggtg cccaaaggtt tcagcccatt gagcctgcgt
     1261 tcgtatctcc gtgtgtcggg aggttttaaa aacatctccg tgtttccgga tccgatcaag
     1321 accggaacgg attccctgtt taagaagctc ttcaatgttg tgacgatgct cgtaatgaca
     1381 ccgttccaac ccagagacct cggccagggc aaggacgtgg actgcgatgc cctgatcgcc
     1441 caggtacaga accaggaccc aaacggactc gtgctcaagg acgtaatgaa accgagcggt
     1501 gaacaaatgg tttcacgcaa gcgaaattcg gcagaacaac ag
//
LOCUS       DASTWE010000961         1212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1254071, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000961 DASTWE010000000
VERSION     DASTWE010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254071"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFT30_10000"
     CDS             <1..1037
                     /locus_tag="VFT30_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEU5093009.1"
                     /translation="KGTKDRIGRLLKMHANKREEIDEVHAGDIVAAVGLKGATTGDTL
                     ADEKQPVLLEIMKFPEPVIAMAIEPKTKPDQEKMGFALQKLAQEDPSFRVRTDEETAQ
                     TIIAGMGELHLEIIVDRMLREFKVEANVGKPEVAFRETIRRKAEAESKYIKQTGGRGQ
                     YGHVVMTVEPSESGKGLEFVNKVVGGAIPKEYIPAIEKGVRERMETGVVAGYPLRDVK
                     VTVIDGSYHDVDSNEMAFKIAASMGFADACKKADPVLLEPIMKVEVLVPQEFMGDVIG
                     NLNGRRGKVQGMKVRAGAQAIEAAVPLMEMFGYATDLRSRTQGRATYSMEFDRYDQVP
                     KNIAEAIIKK"
     gene            1072..>1212
                     /locus_tag="VFT30_10005"
     CDS             1072..>1212
                     /locus_tag="VFT30_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5093010.1"
                     /translation="MAKAKYERKKPHVNIGTIGHVDHGKTTLTAALTKVCADKGMAKF
                     VSY"
BASE COUNT          310 a          238 c          414 g          250 t
ORIGIN      
        1 cgaagggaac gaaggatcgg atcggacgtc tcttgaaaat gcacgctaat aagagagagg
       61 aaatcgatga ggtgcatgcc ggagacatcg tcgctgcggt agggctcaaa ggggccacca
      121 ccggagatac cttggcagat gagaagcagc cggtgttgct tgagatcatg aaatttcctg
      181 agcctgttat tgcaatggcg attgagccga agaccaagcc ggatcaggag aagatgggct
      241 ttgcgctcca gaagttggca caggaagacc cttccttccg tgtgcggacg gatgaagaaa
      301 cggcacagac gatcattgcc gggatgggtg agcttcatct cgaaattatc gtcgatcgga
      361 tgttgcgaga atttaaagtt gaagccaacg tcgggaagcc cgaggtcgca ttcagggaga
      421 cgatcagacg gaaggccgaa gccgaatcga aatacatcaa gcagaccggt ggtcgtgggc
      481 agtatggcca tgtcgtcatg acggtcgagc cgtctgaatc gggtaaggga ttggagtttg
      541 tcaataaggt tgtggggggt gcgattccga aagagtatat ccccgctatt gaaaagggtg
      601 ttcgggagcg gatggaaaca ggagtggttg ccggctatcc cctgcgagat gtaaaagtga
      661 ccgtgattga tgggtcatat catgacgtcg attcgaatga aatggcattt aagatcgcgg
      721 cttcgatggg ctttgcggat gcctgcaaaa aagcggaccc cgtcttgctc gagccgatta
      781 tgaaggtcga ggtcctggtt cctcaggaat tcatggggga tgtcatcggc aatttgaacg
      841 ggcgaagggg gaaggtgcag ggtatgaagg ttcgggctgg cgctcaagcg attgaagcgg
      901 ccgtgccttt gatggagatg tttggttatg cgactgacct tcgatctcgg acgcaggggc
      961 gcgcaaccta cagtatggaa tttgaccgtt acgatcaggt gccgaagaat attgcggaag
     1021 ccatcataaa aaaataggtg gcggttagtg aattagggga gggagtgggt tatggcgaag
     1081 gcgaagtacg agcggaagaa gccgcacgtg aacatcggga cgatcgggca cgtggaccat
     1141 gggaagacga cgttgacggc ggcgttgacg aaagtgtgtg cggacaaagg gatggcgaag
     1201 ttcgtgagct at
//
LOCUS       DASTWE010000962         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_639982, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000962 DASTWE010000000
VERSION     DASTWE010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639982"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..641
                     /locus_tag="VFT30_10010"
     CDS             216..641
                     /locus_tag="VFT30_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093011.1"
                     /translation="MMQTNAVLDLLGDLLAQLKSYASKLPAVVHLASVPSGIKPQPES
                     VEIFEQALSRFREQSGRSPFKGLLDYLIESLEAFEVGNVLGAVQPLLAVLDQIDRMQR
                     DKDVNMTPAEEKRMGDYRAALIKILPGSQPELEGAGRGM"
     gene            1004..>1256
                     /locus_tag="VFT30_10015"
     CDS             1004..>1256
                     /locus_tag="VFT30_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5093012.1"
                     /translation="MSDTKAMLAKTDLVVSAWDGTRLVAFGRVLTDYVYRATIWDVIV
                     DHEYQGRTIGTEIVRRILNHPALHQVELFWLCTRTPAFYE"
BASE COUNT          286 a          340 c          333 g          297 t
ORIGIN      
        1 agggcataca gatcccccgt gtcatgctgc atgcccggct gctagggttc cagcatccaa
       61 ccgggggaag gaaccttctt ttcgaggtgc cttgtcctcc agatatgcag caggtacacg
      121 atttgctcac tgcagtcaca agccattcac attgagtggt ggtgaagaat aggtttgcgg
      181 ctgctcagcg agatcggtcc agtgcaagga gattgatgat gcaaacgaat gcggttctcg
      241 atctgctcgg cgacttgctc gcgcagctga aaagctacgc gtcaaaatta ccggcggtcg
      301 tgcaccttgc ctcagtaccc agcggcatca agccacaacc ggaatcggtg gagatctttg
      361 agcaggcgct gtcacgtttt cgagagcaaa gcggtcgctc tccgttcaag ggcttgcttg
      421 actacctaat cgaatccttg gaagcgtttg aagttggcaa tgtattagga gcggtccaac
      481 cattgctggc ggttttggat caaatcgacc gcatgcagcg cgataaggat gtgaacatga
      541 ctcccgccga ggaaaagcgg atgggcgact atcgcgccgc gctcatcaaa attctgcccg
      601 gcagtcaacc ggaattggaa ggagcaggac gggggatgta actcagtgcc ccattttcgg
      661 tcccgtcgcg tttgcacctg aagtcaccaa ctgaaagcgc accgtcgagc cacagtgggg
      721 acatgtggtt cggactgttg cctcatcgat gacctgatac tggtcttcct taagccacat
      781 caattgaaag cacttaggac agcttcgcac ttgcgttccc atggtcttcc ccttacacta
      841 cgatctatcg attgacccca gattggaggg attaatttag tataagatgc ctcctgctct
      901 caataccaag caccagtcca ttaccttttc agatcgtcac gatttcgacc cgtcgcaact
      961 cctccatctt taccgtcaag ccgcctgggc caagcatcga aacatgagtg acactaaggc
     1021 catgctggcc aagaccgacc tggttgtttc ggcctgggat ggcacacgcc tggtggcttt
     1081 tggacgggtt ctcacggatt acgtgtatcg agccacgatt tgggacgtca tcgtcgacca
     1141 tgagtatcaa gggcgaacga taggcactga gattgtcaga aggattttaa accatcccgc
     1201 gcttcatcaa gtcgagctgt tttggctatg cactcgtacc ccggcctttt atgagc
//
LOCUS       DASTWE010000963         1700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_413534, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000963 DASTWE010000000
VERSION     DASTWE010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413534"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFT30_10020"
     CDS             <1..374
                     /locus_tag="VFT30_10020"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5093013.1"
                     /translation="LASGTGRLARLHGSFVSDDDVRSVVEFVKKQASPIYNQELQSLK
                     LEAAEEEEAKDEVYEQAKDLVLSTGQASASLIQRRLRVGYPRAARMIEQMESEGIVGA
                     AGRDGRREVLGRRGPVGATEV"
     gene            371..1078
                     /locus_tag="VFT30_10025"
     CDS             371..1078
                     /locus_tag="VFT30_10025"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEU5093014.1"
                     /translation="MMHWWLAAPLSVMFVLSAWAADGPIDEKALQEVREVVKQLQARY
                     EKMKDMQADFSQKTRIEGFERPVTSAGKVYIKKPGRLRWDYLDPAIEQIYVNQDDVKV
                     YVPEHKQVLVGKLTQMAASKAPLELLQGAAKLDESFEIEPTPGKDRGVGGLPLITLIP
                     KTKEQESTQNLQKIIVEVFPKTYFIRTVSLHEISGNVAVFEFSNLKPNLGLGNEVFDF
                     KTPSDAEVVRAPVLNGP"
     gene            1202..>1700
                     /locus_tag="VFT30_10030"
     CDS             1202..>1700
                     /locus_tag="VFT30_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU5093015.1"
                     /translation="MTVETGTVVDAVDQAVARLDFDRLRQEYWEQNEFLVIKQFLPRV
                     FVEEVLVPQAQGVKTELNRNYIPGHKKGGSVSYYTVREKATRFLDLYRSESFRTFLNR
                     LVKAELMFCPDNDPHSCALYYYTEPGDHIGFHYDTSYYKGARYTILMGLVDRSTQCKL
                     VCELFK"
BASE COUNT          409 a          407 c          501 g          383 t
ORIGIN      
        1 acctggcctc cggcaccggc cgcctcgctc gtctgcatgg gtcctttgta tctgacgacg
       61 atgtccgctc agtggtggag tttgtaaaaa aacaagcgtc tcccatctat aatcaggaac
      121 tccagtcgct caagttggag gcggcggagg aggaagaggc aaaagatgaa gtgtacgagc
      181 aggcaaaaga cctggtgctg tcgaccggac aagcttcagc ctcattgatt cagcgccgac
      241 tgcgagtcgg ctatcctcga gcggcgcgca tgatcgaaca gatggaatcg gaaggcattg
      301 tgggggcggc gggtcgtgat gggcgtcggg aggtgcttgg tcggcgcggc ccagtcggcg
      361 cgacggaagt atgatgcatt ggtggcttgc agctccattg agtgtcatgt tcgtcttgtc
      421 cgcctgggcg gcagacgggc caattgatga aaaggcgttg caggaagttc gcgaagttgt
      481 caaacaactg caagctcgat acgagaaaat gaaggatatg caggccgatt tttctcagaa
      541 aaccaggatt gaagggtttg agcggcctgt gacctcggcc ggcaaggtgt atatcaagaa
      601 gcccggccgt ttacggtggg actatctgga tccggcgatt gagcaaatct atgtgaacca
      661 ggacgatgtg aaagtgtatg ttcccgaaca taagcaggtt ctggtcggca agttgacgca
      721 gatggctgcc tccaaagcgc cgttggaact gttgcaggga gcggctaaat tggatgagtc
      781 gttcgagatt gaacccaccc cggggaaaga ccgcggagtc ggtggcttgc cgcttatcac
      841 gctcattccc aaaaccaagg agcaggaatc cactcagaac cttcagaaga tcatcgtaga
      901 agtctttcct aaaacatatt ttatccgcac ggtgtcactt catgagatca gcggcaacgt
      961 cgccgtcttc gaattttcga acttgaaacc aaacctggga ttaggtaatg aagtgttcga
     1021 ttttaagact ccgtcagatg cggaagtcgt cagggctcct gtattgaacg ggccctagtc
     1081 gtcagacgaa ccgcgtcaca cgttaatggt cacgcgtcaa actgtggcag aagaagagaa
     1141 tgcttcacga tggtatttgg cgaatgacgg ttgaggctgg gctcaaggag tgatctggga
     1201 catgacagtt gaaacgggca cagtggtcga tgccgtcgat caagcggtgg caagattgga
     1261 tttcgaccga cttcgccagg aatattggga gcaaaacgag ttcctggtga tcaaacaatt
     1321 cttgccccgt gtcttcgtcg aggaagtgct cgttcctcag gcccagggcg tgaaaacgga
     1381 gctcaatcga aactacattc ccggccacaa gaaaggcggg agcgtcagtt attataccgt
     1441 gcgagaaaaa gctactcgct ttctcgacct ctaccggtct gagtcgttcc ggacattcct
     1501 gaaccggctc gtgaaggcag agctgatgtt ctgtccggac aacgatcccc attcctgcgc
     1561 cctttactac tacactgaac cgggtgacca tattgggttc cactacgata cgtcatacta
     1621 taagggtgct cgttacacga tcttgatggg gcttgttgat cggtcaactc agtgcaagtt
     1681 ggtgtgcgag cttttcaaag
//
LOCUS       DASTWE010000964         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_801524, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000964 DASTWE010000000
VERSION     DASTWE010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801524"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFT30_10035"
     CDS             complement(<1..558)
                     /locus_tag="VFT30_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU5093016.1"
                     /translation="MAEGVAFIEGQYVPSDEAKISVFDLGFSRSDAVYDVVSTWKSLF
                     FRLDDHIDRFLRSCAGVRIACPYPKEEIKRILAECVHRAGLQDSYVEVLTTRGRFETV
                     GSRDLRKTKPTFMAYAIPYVWIAPLEKQREGLHMHIAQTLRIPDVSVAARFKNFHWAD
                     LTQGQLEALDAGADVAVLCGVTGHLS"
     gene            605..1354
                     /locus_tag="VFT30_10040"
     CDS             605..1354
                     /locus_tag="VFT30_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5093017.1"
                     /translation="MSYETILVEQKNGVATIIMNRPEVLNAITATMLTELKHAVDEAG
                     ADLAIGVVVLTGMGRAFSAGVDLKALGKLPLENGAIGPILDDPARALIRSIQTIPKVV
                     IAKINGFCFTGALELALACDLMVVAEDAKFGDTHAKWGLRPTWGMSARLPQAAGFRKA
                     RELSFTADTFTGRDAVAWGVANRAVPPAELDAVVQALATKILANSRDSLAAYKALYNH
                     GSGKTVSEAVAFEEASVFSISDSNERVEKFR"
     gene            complement(1449..>1995)
                     /locus_tag="VFT30_10045"
     CDS             complement(1449..>1995)
                     /locus_tag="VFT30_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093018.1"
                     /translation="DLLDRLALLAEQLRDGKDLKIVWDCPLPLPTMETDGLRLEQILT
                     NLVTNAVKFTQKGQITVRVRQALVQGLVIFEVSDTGIGIPTKELPYIFDEFRQVDGSS
                     SRSYGGMGLGLALVKKLATLLQGEISVISQLGQGSIFTVTFPLRLPPPAPTAEAQHLT
                     PQQDNAAGSAKRGKEAEKHVS"
BASE COUNT          429 a          562 c          559 g          445 t
ORIGIN      
        1 agacagatgt cccgtcacgc cacacaacac agccacatca gcaccggcat cgagcgcttc
       61 gagttgccct tgcgtcaagt ccgcccagtg aaaatttttg aagcgagcgg cgactgacac
      121 gtccgggatg cgcaacgtct gcgcaatatg catgtgcagt ccttctcgtt gtttctctaa
      181 cggagcgatc cacacatacg ggatcgcgta ggccataaag gtcggctttg tcttacgtaa
      241 atcgcggctg cccacagtct cgaatcggcc gcgcgtcgtc agcacttcca cgtaggagtc
      301 ctggagtcct gcacgatgga cacattcggc aagaatccgt ttgatttcct ccttcgggta
      361 gggacatgcg atacgtaccc ccgcacacga ccggagaaaa cgatcgatat gatcgtcgag
      421 gcggaaaaac aggcttttcc aagttgacac gacgtcatag acggcatcgc tccgactgaa
      481 gccgagatca aacaccgaga tctttgcctc atcgctcggt acgtactgac cctcgatgaa
      541 cgcgacacct tcggccattg ctcctccctt ttgcttatgg ttagtttctc tcaaaggaag
      601 acctatgtca tacgaaacga ttctcgtcga gcaaaaaaat ggcgtcgcga ccattatcat
      661 gaaccgcccg gaggtactca acgctatcac cgcaacgatg ctcaccgagc tgaagcatgc
      721 ggtcgacgag gcgggtgctg acttagccat tggtgtggtc gtgctgaccg gcatgggccg
      781 ggcattctct gccggtgtcg atttgaaggc gctaggaaag cttccgctag aaaacggcgc
      841 gatcggtccg attctcgacg accccgcacg cgcgttgatc cgctcaattc agacgattcc
      901 caaagtcgtg atcgccaaga tcaacggatt ctgtttcact ggcgcactcg aacttgcctt
      961 ggcctgtgac ctcatggtgg tcgccgagga cgcgaagttt ggcgacacgc acgccaaatg
     1021 ggggttacgg ccgacgtggg gcatgagtgc gcgcctgccg caggcggccg gtttccgcaa
     1081 ggctcgcgaa ttatcgttca ctgccgatac gttcactggt cgtgacgccg tggcgtgggg
     1141 cgtcgcgaat cgcgccgtcc cgccggccga actcgatgcg gtcgtccagg ctctggcgac
     1201 caagatcctt gcgaatagcc gcgactctct cgcggcctat aaagcgctct ataaccacgg
     1261 gagtgggaaa actgtgagtg aggcggtcgc ctttgaagaa gcaagcgtct tctctatttc
     1321 cgactccaat gagcgtgtgg agaagtttcg ttaggaaaat tcagctatca atgtcttcct
     1381 ctcagacctg tcaccctgag tgaaacgaaa ggttgctcgg agggattctt cgtagcgcgc
     1441 ggcgagttct acgacacgtg cttttcggct tctttacccc ttttggcgga tcctgcggca
     1501 ttgtcttgct gcggcgttag gtgctgtgct tcggcagtgg gtgccggcgg aggaaggcga
     1561 agcgggaagg tgacggtgaa gattgaccct tgcccgagct gactgataac tgagatttcc
     1621 ccctggagaa gcgtggcaag tttctttacc agtgccaagc ccaatcccat cccgccgtag
     1681 ctccgactgc tggagccatc cacttggcgg aactcatcaa agatgtacgg aagctctttc
     1741 gtagggatgc caatacctgt atcggacact tcaaaaatga cgagaccttg caccagggct
     1801 tgacgaacac gcacggtaat ctgccccttt tgcgtgaatt tgaccgcatt ggtcaccaga
     1861 ttggtgagaa tctgctcgag acggagaccg tcggtttcca ttgttggtaa gggcagtgga
     1921 cagtcccaca cgattttgag gtctttgccg tcacggagtt gctccgccaa cagtgctaga
     1981 cgatcaagaa gatct
//
LOCUS       DASTWE010000965         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1480390, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000965 DASTWE010000000
VERSION     DASTWE010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480390"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFT30_10050"
     CDS             <1..180
                     /locus_tag="VFT30_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093019.1"
                     /translation="QKDVLEAFRDKIEMIVAKVAKRLGLQVIVDKSKGGPTIYHEEGL
                     DISGPVIEEFNREYP"
     gene            200..742
                     /locus_tag="VFT30_10055"
     CDS             200..742
                     /locus_tag="VFT30_10055"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU5093020.1"
                     /translation="MTRAVKMMAVVVALLLAQWVEPLLAGEVVRVGVMDQQMVMERTK
                     GGKLALEDVKGYSLTRQKIINADEQELKDLDQSLQDPNAKLTDQARQEKEEQLRSKME
                     AYQRRLQEFNREVQQKQREMVAEYAQKIAAAAKTVAQKEGYTAILDKGNEALIRIVLY
                     YQPALDVTDSVIKEFDRQNP"
     gene            781..1233
                     /gene="fabZ"
                     /locus_tag="VFT30_10060"
     CDS             781..1233
                     /gene="fabZ"
                     /locus_tag="VFT30_10060"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU5093021.1"
                     /translation="MASVEPAEIQALLPHRYPFLLVDRVKEFEPHKRIVGIKNVTVNE
                     PFFQGHFPGRPVMPGVLIIEAMAQVGGVLVFKSGGPVGKTVMYLTGIDEAKFRRPVVP
                     GDQLRFEIDVLKKRPPFWKMQGKAYVDNEVVCEAVVTAMVMEEKVETR"
     gene            1334..>1716
                     /locus_tag="VFT30_10065"
     CDS             1334..>1716
                     /locus_tag="VFT30_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HEU5093022.1"
                     /translation="MKIHPTAIVHPKASLDGDVEIGPFCVIGEHVAIGKGSRLLSHVT
                     IDGWTEIGERNEVHPFASIGGPPQHLGYKGEPTKVIIGHDNIIREYVTINRGTAQGGG
                     VTSLGSKGILMAYVHVAHDCRLGNH"
BASE COUNT          442 a          374 c          542 g          358 t
ORIGIN      
        1 caaaaagacg ttctggaagc gtttcgtgac aagatcgaaa tgatcgtcgc aaaggtcgcc
       61 aaacgtctcg ggttgcaggt tatcgttgat aagagcaagg gtggtccgac catctatcat
      121 gaagaagggc tggacatttc aggcccggtc atcgaggagt tcaaccgcga atatccctag
      181 tcgctgcggg aggtagatca tgacgagagc ggtgaaaatg atggcggtgg tcgtcgcgtt
      241 gttgctggcg cagtgggttg agccactcct tgctggtgaa gtggtccggg tcggcgtgat
      301 ggatcaacag atggtgatgg aacgaacgaa gggcgggaaa ctggccttgg aagatgtgaa
      361 gggatattcc ttgaccagac agaagatcat taatgccgat gagcaagagc tcaaagattt
      421 ggatcagtcc ttacaagatc cgaacgccaa actcaccgac caggcgagac aagagaaaga
      481 ggagcagttg cggagtaaga tggaagcgta tcagcgccgc ctgcaagagt ttaatcgcga
      541 agttcaacaa aaacagcgcg agatggtcgc agaatatgcg caaaagatcg cggccgcagc
      601 gaaaactgtg gctcagaaag agggatatac ggcgatcctc gataaaggca atgaggccct
      661 catccgcatc gttctctatt atcagccggc gctggatgtg acggactcgg tcatcaagga
      721 atttgatcgg cagaatcctt aaatcgtaga actatcaggg acagagagga ggcagagcca
      781 atggcatcgg tcgagccggc tgagattcag gcgttacttc cccaccggta tcctttctta
      841 ttggtagatc gagttaaaga gtttgaaccg cataaacgga ttgttgggat caaaaatgtg
      901 acggtcaatg agccgttctt tcaaggacat ttcccgggac gccctgtcat gccgggggtg
      961 ctgattatcg aagccatggc ccaggtgggt ggcgtgctgg tcttcaagtc gggagggccg
     1021 gtcggaaaaa ccgtcatgta tctgacgggt atcgatgagg caaaattccg tcgaccggta
     1081 gtgccgggcg atcagctccg ttttgagatc gacgtactca agaagcgtcc gccgttctgg
     1141 aaaatgcaag ggaaggcata cgtagacaat gaggtggtct gtgaagctgt ggtgacagcg
     1201 atggtgatgg aagagaaagt ggaaacacgg taagcggtga tcgttcctcg atttctgaaa
     1261 gcgtgtggtg agcaagccag tgaatagttg gccaatgacc ggtggtgctt ggcgctagta
     1321 taagggggca tgcgtgaaga tacatcctac agcgatcgtt catcccaagg cgagcctcga
     1381 tggtgatgtg gagatcgggc cattctgtgt aatcggggag catgtcgcga tcggaaaagg
     1441 aagccgattg ctctcccatg tgactatcga tgggtggacg gagattggtg agcggaacga
     1501 agtacacccg ttcgcttcga tcggagggcc tccacaacac cttggatata aaggggagcc
     1561 gaccaaggtc atcatcggcc atgacaacat tatccgggag tatgtcacga tcaacagggg
     1621 taccgcccaa ggggggggag tgacctctct tgggtcgaag ggcattctga tggcctacgt
     1681 gcatgtggca cacgattgcc ggctcggcaa ccatat
//
LOCUS       DASTWE010000966         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_478695, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000966 DASTWE010000000
VERSION     DASTWE010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478695"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..>1576)
                     /gene="acs"
                     /locus_tag="VFT30_10070"
     CDS             complement(42..>1576)
                     /gene="acs"
                     /locus_tag="VFT30_10070"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5093023.1"
                     /translation="NRCANALKGLGLQKGDRVTIYLPKIPEQVVAMLACARIGVIHSV
                     VYSGFSSPALASRIQDAEARVVLTADVGYDRGKTINLKGVVDDAVRTCSSVEKVVVVR
                     RAPDTVPLTAPKEIDWTEWLTAQSALCPAEPLDSETPLYILYTSGTTGKPKGVVHVHG
                     GYMVGTYITTKYVFDLKEEDVYFCVADPGWVTGHSYIVYGPLLNGATILMAEGKPDYP
                     NPGRWWDLIERYGVSIFYTTPTAIRLLMKYGEEWPKKSDLSTLRILGSVGEPINPEAW
                     EWFYRVTGSDKPIMDTWWQTETGSILITPLPSVPLKPGSATRPFLAIEADVVDREGHS
                     LPPNAGGFAVIKRPWPSMMRTIYKDPERYKVYWNTIPNCYTAGDVCRKDRDGYMWFMG
                     RADDVIKVAGNRIGTAEVESALVSHEAVAEAAVIGKPHRTAGEAIKAFVILKQGHQDS
                     KELVQSLKDQVLKELGKIAVPQEIDIVPSLPKTRSGKIMRRVLKAKELGQDVGDISTI
                     ED"
BASE COUNT          316 a          457 c          452 g          351 t
ORIGIN      
        1 atgtgacagt aaaattgacc caccgactga acatgatcgg ctcagtcttc gatcgtcgaa
       61 atgtcgccca cgtcctggcc caattccttg gccttgagga cccgccgcat aattttgcct
      121 gagcgcgtct tcggcaatga cggcacgatg tcgatttcct gcgggacggc aatctttccc
      181 agttccttca agacctgatc cttcagcgat tgcaccaact ccttcgagtc ctgatgcccc
      241 tgtttcaaaa tgacaaaggc cttgatcgct tcaccggctg tgcgatgggg tttgccgatc
      301 accgccgcct cggcgacggc ctcatggctg accaaagcgc tttcgacctc cgcggttccg
      361 atgcggttac cggcgacctt aatcacgtcg tcggcgcggc ccatgaacca catgtagccg
      421 tcacgatctt ttcggcacac gtctccagcc gtgtagcagt tgggaatcgt gttccaatag
      481 actttgtagc gttcggggtc tttgtaaatc gtccgcatca tcgatggcca gggccgcttg
      541 atgacggcaa agccgccggc attcggaggc agactgtggc cttccctatc caccacgtcg
      601 gcttcaatgg cgagaaacgg cctcgtggca gaacccggct tgagcggaac ggagggcaga
      661 ggcgtaatca gaatggagcc ggtttcggtt tgccaccagg tgtccataat gggcttatcg
      721 cttccagtga cgcgataaaa ccattcccag gcttccgggt tgatgggttc accgacgctt
      781 ccgagaatgc gcaaggtcga gagatctgat ttcttgggcc actcctcgcc atatttcatc
      841 agcaaccgga tggctgtcgg cgtggtgtag aagatggaga ccccgtaccg ttcgatgaga
      901 tcccaccatc ggccgggatt aggatagtcg ggtttcccct cggccatgag tatggtggcg
      961 ccgttcaaca gcgggccata gacgatgtag ctgtgccccg tgacccagcc cggatcggcc
     1021 acacaaaaat agacatcctc ttccttcagg tcgaaaacgt atttcgtggt gatgtaggtg
     1081 ccgaccatgt acccgccgtg gacatgcacc acgccttttg gtttgccggt ggtccccgag
     1141 gtgtacagaa tatagagggg agtttctgaa tccagcggtt cggcggggca aagagccgat
     1201 tgagccgtca gccattccgt ccagtcgatt tctttcggcg ccgtgagcgg gactgtgtcg
     1261 ggggcgcgac gcaccactac gaccttttca acggacgagc aggtacgcac ggcatcgtct
     1321 acgacacctt tcagattgat ggttttcccc cggtcgtagc ccacatctgc cgtgaggacg
     1381 acccgtgctt cggcatcttg aatgcgactg gcaagggcgg gggagctgaa accggagtac
     1441 acgacgctgt gaatgacgcc aatgcgggca caggccagca tggcaacgac ttgctctgga
     1501 atcttcggga gataaatggt cactcggtcg cctttttgca gccccagccc cttgagagcg
     1561 ttggcgcaac gattga
//
LOCUS       DASTWE010000967         1318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_413573, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000967 DASTWE010000000
VERSION     DASTWE010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413573"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFT30_10075"
     CDS             complement(<1..936)
                     /locus_tag="VFT30_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5093024.1"
                     /translation="MLSTNTTPFKAPLPPPDQTLIAQAVETRLPPGLILRALTPLAGD
                     ASNRRYYRAAVAGGPPHSVIVMQLAEPEGFKQSEEAVSGAIHQISELPFVNIMGHLAK
                     ANVPVPALHYYDQSAGLLYLEDFGDVTLAEAVSQADAPSLESRYKQAINVLVQMQINA
                     TTPADPRCLAFHRSFDVPLLMWEFDHFLEYGIVARSGKPLSDEEATAIKREFDWIAEL
                     LAGQPRVFTHRDYHSRNLMVDGVRLGVIDFQDALMGPATYDLASLVRDAYIQLDEALV
                     DDLVEYYLDQLAERRFVWTNRAAFRRLFDLTSIQRN"
     gene            complement(996..>1318)
                     /locus_tag="VFT30_10080"
     CDS             complement(996..>1318)
                     /locus_tag="VFT30_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU5093025.1"
                     /translation="ETHVPFVTHFSDKVLHIAEYAVLGALCYRAIRGSGNDSWRGLAI
                     PAAILLASLYGVSDEIHQAFVPFRDSSWLDWLADTVGAALGVTLLHRMLNLRPVGSIP
                     ETLP"
BASE COUNT          312 a          375 c          352 g          279 t
ORIGIN      
        1 attgcgttga atgctcgtca aatcaaacag tcgtcggaac gcggcgcgat tggtccagac
       61 gaaacgtcgt tcggccaact ggtcgagata gtactccacc aaatcgtcga caagggcttc
      121 gtcgagttga atgtaagcgt cccgcaccag tgaggcaaga tcgtaggtcg ccggtcccat
      181 taaggcatcc tgaaagtcga tcacgccgag ccgcaccccg tcgaccatca aattgcggga
      241 gtggtaatcg cgatgcgtga agacgcgtgg ctgcccggct agtagttccg caatccaatc
      301 gaactcccgc tttattgccg tggcctcctc atcggacaat ggtttaccgc tacgcgccac
      361 gattccatac tccaggaagt gatcaaattc ccacatcaat aacggcacgt cgaagctacg
      421 gtgaaaggct agacatctgg gatccgccgg cgtcgtagca ttgatctgca tctggaccaa
      481 gacgttgatg gcttgcttgt accgtgactc caagctcggt gcatccgcct gactgacggc
      541 ctctgccaac gtcacatccc cgaaatcttc cagatagagc agccctgcgg attgatcata
      601 gtagtggagt gccggcactg gcacattcgc cttggccaaa tgccccatga tgttgacgaa
      661 tggtagttcg gagatctggt gtatcgcccc gctcaccgcc tcttctgact gtttgaatcc
      721 ttccggctca gccagctgca tcacgatcac agaatgtggt ggtccgcccg ccacggcggc
      781 tcgatagtag cgacgattgg aggcatctcc tgccaaaggc gtcagggctc tcaagatgag
      841 gccgggagga agacgagtct cgacggcctg agcgataagt gtttgatcgg gaggaggcag
      901 cggagctttg aaaggggtgg tgttggtcga caacatactc atcaaattat agcgagcgtc
      961 ccgcgacttg tcacgaatac cacgtattcg atctactacg gcagcgtttc cgggatcgac
     1021 cccaccggtc tgagattcag catccgatgc aagagggtca ctccgagtgc ggccccgacc
     1081 gtatctgcca gccaatcgag ccaacttgaa tctcgaaatg gaacgaaagc ttggtgaatt
     1141 tcgtcgctca ccccatacaa cgaggccagt agaatggccg ctggaatcgc tagccctctc
     1201 cacgaatcat ttccacttcc acgaatagct cgatagcaca acgcacctaa tacggcatac
     1261 tcggcaatat gcaagacctt atcgctaaaa tgagtgacga acggtacatg ggtttcag
//
LOCUS       DASTWE010000968         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_90603, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000968 DASTWE010000000
VERSION     DASTWE010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90603"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFT30_10085"
     CDS             complement(<1..202)
                     /locus_tag="VFT30_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093026.1"
                     /translation="MNLWHRIINVSLGILLCAGVASAVEPAEVEKFVNARIEIGEMMT
                     NYFKGGQSYGEGQRPSQEQMRQM"
     gene            complement(310..1755)
                     /locus_tag="VFT30_10090"
     CDS             complement(310..1755)
                     /locus_tag="VFT30_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5093027.1"
                     /translation="MKGLTILLVDDEPLMRLSMMDALGAVGCDVRAVPTGTEGIEAIR
                     EKTFDVVITDLRLPGVDGLTVLQTAKGKDPQTEVLVITAHGSVETAVEAMKLGAFDYI
                     TKPFQMDELLLIVERLSSMITLRRENQDLKHQLEDKFCFNGILGANSQMRAVLDKIKL
                     VAETDSTVLIVGESGTGKELVANALHQNSSRKGYPLIKVSCAALPETLLEAELFGHEK
                     GAFTGALRQRRGRFEMANRGTLFLDEIGEISPVVQVKLLRVLQERTFERVGSNESIET
                     DVRLVCATQKDLRKEVAQGRFREDLFYRLNVVPIVVPPLRQRQEDIMVIADHVLETCS
                     VKLNKHLRGFSQQARELLLRYSYPGNVRELENMVERAVALGRDRTAVQPADLCGFQAC
                     PYLGGVQQESCGFCSEGLTGGKKKKDTTLTSLIAARESFEKDYIVSVLERVDGSRTTA
                     SRILGLSRKALWEKCKRYGIPSAHSDAEDPT"
     gene            complement(1752..>2305)
                     /locus_tag="VFT30_10095"
     CDS             complement(1752..>2305)
                     /locus_tag="VFT30_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093028.1"
                     /translation="MEHGVRRVQKIVRQLLDFSQQHEPAFSPADINRIIDQVLGLTTH
                     LFGPNRIRLETFLGNGLPNVMVDRHMIEQVLMNLILNAVQAMKNGGTLTIRTSVVEGI
                     CRVEVNDTGSGIPASVLPRVFDPFFTTKGEGEGTGLGLSVNLGIVERHGGKILVESEV
                     GKGTTFTLCLPVLRERFFAEKEA"
BASE COUNT          502 a          686 c          565 g          552 t
ORIGIN      
        1 ccatttgtcg catttgctcc tgggacggac gctgcccctc cccatagctc tggccgcctt
       61 tgaaatagtt cgtcatcatc tcaccgattt cgatccgcgc gttgacgaac ttttccactt
      121 cagcgggctc gacggcagag gctactcctg cgcatagaag aatgcccagt gaaacattga
      181 tgattcgatg ccacagattc atagcttata tccccctcca ccaagtagat taattctaca
      241 taatacgaca acttcgtttc cggattgttc aacaattcgc actcccccta gcatagtgaa
      301 cggaccgttt taggttggat cctcggcgtc actgtgtgcc gagggaatac cgtagcgttt
      361 gcacttttcc caaagagcct ttcgggacaa tcccaagatt ctcgaggcgg tcgtgcggct
      421 cccatcgaca cgttccaata cggagacaat gtagtccttc tcaaaacttt ctcgcgcggc
      481 gatcagcgaa gtcagggtcg tatctttttt ctttttcccg ccggttaaac cttcactaca
      541 gaaaccacag gattcttgtt gaacgccccc caaataggga caggcttgaa atccacagag
      601 gtcggccggt tgaaccgcgg tccgatctcg cccgagggcc acggcccgct cgaccatgtt
      661 ttctaactcc cgcacattcc ccggatacga atagcgaagc aataattcac gcgcctgctg
      721 ggaaaacccg cgcaggtgct tgttcagctt aaccgagcag gtttcgagga catgatcggc
      781 aatcaccatg atgtcttctt gtcgttgcct gagcggggga acgacgatcg gtacgacgtt
      841 gaggcggtaa aagaggtctt cacggaaccg gccctgagct acctctttgc gtaggtcttt
      901 ctgtgtggcg cacacgagcc gtacgtccgt ttcaatcgat tcattgctcc cgacccgttc
      961 gaaggtgcgt tcctgcagga cgcgcaagag tttcacctgc accacaggcg agatttcacc
     1021 gatttcgtca aggaacaagg tccctcgatt cgccatctcg aatcttcccc ggcgctggcg
     1081 cagggcaccc gtaaacgcac ctttctcgtg tccgaagagt tccgcttcaa gcagtgtctc
     1141 cggcaacgcg gcgcagctga ctttgatcag cggataccct ttccgggaac tgttctggtg
     1201 cagcgcattg gcgacaagtt ccttgcccgt gccgctctcc ccgacgatca ggacggtgga
     1261 atcggtctca gccacgagct tgatcttgtc caatacggcg cgcatctgac tgttcgcccc
     1321 taaaatgccg ttgaaacaga acttgtcttc aagctggtgt ttgagatcct gattctcccg
     1381 acggagtgtg atcatgctgc tgagccgttc aacgatcagg agcaattcat ccatctggaa
     1441 cggctttgtg atgtaatcaa atgctcccag tttcatggct tcaacagctg tttctactga
     1501 tccatgggcg gtaatcacga gcacctcggt ctgggggtcc ttgcctttag ccgtctggag
     1561 cacagtcagc ccatccacac ctggcaaccg gaggtcggtg atcaccacat cgaacgtctt
     1621 ttctcgaatc gcctcgatcc cttccgtacc ggtcggtacc gcccggacat cacaaccgac
     1681 agctcccaat gcgtccatca tggaaagccg catcaagggc tcatcgtcga ccagcagaat
     1741 ggtcaaccct ttcatgcttc tttctccgca aagaaacgtt ctcgcaacac aggcaggcac
     1801 agtgtaaagg tcgttccttt tccgacctcg ctctcgacca aaattttccc gccatgacgt
     1861 tccacaatgc caagattgac cgagagcccc agccctgtcc cttccccttc tcctttggtt
     1921 gtgaaaaaag gatcaaagac ccgcgggagg acagaggcgg gaatgccgga gcccgtatcg
     1981 tttacctcga cccgacagat gccctcgacc acggatgtcc taatcgtcag tgttccgcca
     2041 tttttcatgg cttgcacagc gttcaagatc aagttcatca agacctgttc gatcatatgc
     2101 cgatcgacca tcacgttagg caagccattg cctaggaatg tttccaggcg aatccgattg
     2161 ggaccaaaga gatgggtggt cagccccagt acttggtcga tgattcgatt gatatcggcg
     2221 gggctgaatg ccggctcgtg ctgctgtgaa aaatcgagca attgccgaac gatcttttga
     2281 acacgtcgga cgccatgctc catgg
//
LOCUS       DASTWE010000969         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_543283, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000969 DASTWE010000000
VERSION     DASTWE010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543283"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFT30_10100"
     CDS             complement(<1..372)
                     /locus_tag="VFT30_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5093029.1"
                     /translation="MTPVQAVIAKELRGYFVSPIVYVVGAVFLLIFGLLSYLYVGFAG
                     AQAIQLMQMQGGMAQINLNDLVFRNLFASMRIVLVIILPILTMRLFAEERKLRTFEFL
                     MTSPIRINEIIVGKFVSVFLIY"
     gene            complement(380..1366)
                     /locus_tag="VFT30_10105"
     CDS             complement(380..1366)
                     /locus_tag="VFT30_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5093030.1"
                     /translation="MIDVQNITKRYGHHTAIDRVTFSVAKGEVLAFLGPNGAGKTTTM
                     RILTCFMPATEGAARVAGFDCTEQPLEVKRQIGYLPETPPVYQELTVTEYLTFVGRLR
                     GITGPKLSSALDQSIGRLELGSVRHRLIGNLSRGYRQRVGLAQALLHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIINKGHIVAEDTPEQL
                     SARLRQSEKVSITLKTCPADCEMKLRTIPGVLNVLPGQAGGSFLIECELGRDLRDEIA
                     RVAVLNQWGLLELRTVSMTLEDVFLQLTRHEDHVSEPSEATVSMASAERTDA"
BASE COUNT          373 a          430 c          453 g          333 t
ORIGIN      
        1 atagatcaag aacacactga cgaatttgcc aacgatgatt tcattgatcc tgatcggcga
       61 ggtcatcaag aattcaaagg tgcgcagctt gcgctcttcc gcgaacagcc gcatggtcag
      121 gatgggcaga ataatcacga ggacgattcg catgcttgcg aagagattcc ggaagaccag
      181 atcgttcaag ttgatctggg ccatgcctcc ctgcatctgc atcaattgga tggcttgcgc
      241 accggcgaaa ccaacgtaaa ggtaggagag cagcccgaag atcaacagaa agaccgcgcc
      301 gaccacgtag acaatggggg agacgaaata accgcgcagt tccttggcga tgacggcctg
      361 taccggggtc atagtgattc tacgcgtccg tcctttcggc agaggccatg ctgaccgtcg
      421 cctccgacgg ctcggagacg tgatcctcgt ggcgcgtgag ctgaaggaag acgtcttcca
      481 acgtcataga cacggtgcgg agctcaagca atccccactg gttcaaaaca gctacgcgag
      541 cgatctcgtc ccgtagatca cgacccagct cgcattcgat gaggaaactt cctcccgctt
      601 gcccaggaag aacattgaga acgccgggaa tggtgcgaag cttcatctca cagtccgccg
      661 ggcatgtctt gagggtaatc gaaactttct ccgattgacg caatcgcgcc gacaactgtt
      721 caggggtatc ttccgcgacg atgtgccctt tattgatgat gaccacccgt tggcacactg
      781 cggtcgcctc cgggaggata tgagtgctga gaattacgga atgcgagccg gccaggctct
      841 tgatgagctc tcggatttca atgatctgct tgggatcaag gccgaccgtc ggctcgtcga
      901 ggatcagcac cgggggatcg tgcaacagcg cttgcgccag ccccacgcgc tgacgatagc
      961 cacgggaaag gttgccgatc agtcgatggc ggacggaacc cagttcaagt cgtccaatag
     1021 attggtcgag cgccgacgag agtttcggtc ccgtgattcc acggagtcga ccgacgaagg
     1081 tcagatattc cgtcaccgtg agttcttggt agaccggcgg agtttccggc aagtaaccga
     1141 tctgccgttt cacctccaat ggctgttcgg tgcaatcaaa acccgcgact cgtgccgcgc
     1201 cttctgtcgc cggcatgaag caggtcaaga tccgcatggt ggttgttttg cccgcgccgt
     1261 tgggaccaag gaacgccaac acctcaccct tggccaccga gaaggtgacg cgatcaatgg
     1321 ccgtatgatg gccataccgt ttggtaatgt tttgaacgtc gatcacgaat gtggtatcgg
     1381 tgcgaacact gtggagaatg gagcggtacc gctctcgaaa ctacaccata ttgatggtgg
     1441 atcttactca ctccgtccga ggcagtcaat atagagtgag gtatttagct ggtggatcgt
     1501 ctataccatg tcaggaacgt gacagctcga aggttgcttc ctaactcgga tagatggcat
     1561 catctccgcg aagaaacgcg cttcgaaaa
//
LOCUS       DASTWE010000970         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_801587, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000970 DASTWE010000000
VERSION     DASTWE010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801587"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VFT30_10110"
     CDS             complement(<1..1317)
                     /locus_tag="VFT30_10110"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5093031.1"
                     /translation="MAGLAATPELLSTLHNKATQLRINSVRATSEAGSGHPSSCASAA
                     DIVAALFFSVMRYDPHNPKAPNSDRFILSKGHAAPLLYAAWAEAGLFPQNDLLKLRTL
                     SSDLEGHPTPRLTFVDMATGSLGQGLPVGIGIALNAKFVDRLDYRTYVLMGDGESVEG
                     SVWEAAEIGRQYGLDNLCAIVDVNRLGQSDPTMLQHDMDAYRSRWAGFGWHAIVVDGH
                     DFTAILKAFDEAARTKGQPTVLLARTYKGKGISFIENKADWHGKPLKKGEETQKALDE
                     LTKQLSPNGAVIQIPKPSAPSTAPVAIGPMPATPYKIGDSVATREAFGAALEALGSVN
                     PLIVALDADVKNSTYTDKFGKKFSNRFFESFIAEQNMVGAAAGLAACGKIPFAATFAC
                     FFSRAYDFIRMAAISGSNIKLVGTHVGVSIGEDGPSQMGLEDIAMMA"
BASE COUNT          368 a          408 c          446 g          358 t
ORIGIN      
        1 cgccatcata gcgatatctt ccaaacccat ttgtgatggc ccatcttcgc cgatgctgac
       61 accgacatgc gtcccgacca gcttaatgtt tgaaccactg atcgcagcca tacggatgaa
      121 gtcgtaggca cgactgaaga aacaggcaaa ggtggcggca aaaggaattt tgccgcacgc
      181 agccaaaccg gcggcggccc ccaccatatt ttgttcggcg atgaaactct caaagaaccg
      241 attggaaaac ttcttgccga atttgtcggt gtacgtggag ttttttacat cggcgtctag
      301 tgcaacgatc aaagggttca ctgaacccaa agcttccaat gccgcaccga aggcttcacg
      361 cgttgcgacg gagtcaccga ttttataagg tgtggccggc attgggccga tggctacggg
      421 agcggtgctg ggggccgatg gtttggggat ttgaatcacg gcaccgttcg ggctcaattg
      481 tttcgtgagc tcgtcaaggg ccttctgagt ctcctcgcct tttttaagtg gtttgccgtg
      541 ccagtctgcc ttgttctcga tgaacgaaat gcctttgcct ttgtacgttc gggccagcaa
      601 cacggttggt tgtcctttgg tgcgggcagc ctcatcgaat gccttgagaa tggctgtgaa
      661 atcatgaccg tcgacgacga ttgcatgcca accgaatcca gcccaccggg aacggtaagc
      721 atccatgtcg tgctgcagca tggtgggatc actctgaccc aatctgttca catcaacaat
      781 ggcgcacaaa ttatccaaac catactggcg gccaatctca gcggcttccc agaccgatcc
      841 ttcgacggat tctccatcac ccatcaatac gtaggttcga taatcgagtc tgtcgacgaa
      901 tttcgcattc aatgcgatgc caatcccaac gggaagccct tgcccaagcg aaccggtggc
      961 catatcgaca aaggtcaagc gaggagtcgg gtgtccttcg agatcggacg ataaggtacg
     1021 caattttaag aggtcgttct gtggaaagag gcccgcttcc gcccatgccg cgtaaagcag
     1081 cggagctgca tgtccttttg acaggataaa gcggtcactg ttgggcgcct ttggattatg
     1141 gggatcgtaa cgcatgacgg agaaaaacaa ggcggcgacg atgtcggcgg cagatgcaca
     1201 gctcgacgga tggccgctcc cagcctcact ggtggcccgc acactattga tgcggagttg
     1261 ggtagcctta ttgtgtaagg tgctgagtaa ttcaggcgta gcagctaaac cggccatggg
     1321 aaatcctttc gggatagaga tcaaaggacg tgtagtagta cctgattgta tgctctcggc
     1381 agtagcctga cagaacttga ggctaacaaa tcagctgggc agaggtcaat gaagagatga
     1441 gctcccttcc acccacttta agaaatgttt ggttcatggc gtggcttctc ggcgggcaac
     1501 cgtaccgaat cgatgccaag ggatgaggcg ccgatggtgc gagtgtgcat gggagtcaag
     1561 ccggtctcgg cagacgcact
//
LOCUS       DASTWE010000971         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1512811, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000971 DASTWE010000000
VERSION     DASTWE010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512811"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..>2070
                     /locus_tag="VFT30_10115"
     CDS             141..>2070
                     /locus_tag="VFT30_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEU5093032.1"
                     /translation="MSEELRQKGYLSPEGAVAGDPAGPFQAFNLGASTINQLARVRIV
                     PRHDYRKRGILKPDGLVVSGVGSAPTVHLVVEFKDRGELDTDAKLDRVLAKVATEYCA
                     PLDCALAVVSDGAVVRWVAVDAGSNTWRIINRDDGYPLDALVDLSSLDGREDVARTIS
                     RVTSELNPATGVLEPAETVDPTRLADQTWQAIWLASGENPERCLASFIEILLFKFLSD
                     LKILTEDRAGRATGFEHVVSLPPSEGLAYYFEVVRPEIIHLFPKGEDGTTVIGGIVLE
                     PKNLDHGRLFTEILRKFDKAGPLKRIDPEFKSRIFERFLKKSISQKNWGQYFTPRNVV
                     KAMVEMSGVDRMSPGEVVADPFCGVGGFVLEPLVHKRPHDFRSSKDRALSYRGYDRDA
                     KTITLAKANMLIHLSEVLEREPEIAPERLAPALNESFHAMDESITGSLELAPKEEWDL
                     VMTNPPYVVTGTTTQRKMLSENPILAAYYSISGSGVENLCLQEIVAGLKAGKRALMIV
                     PDGLLLRHSEESLKRHLLRTCFLECVISLPVDTFYSTPKKTYILVFRKKLDDRELQQD
                     GVLTYLVGEVGETRDAKRFVTTKNHLPEAVSSFKKFQADPGSFSGDQPVERLEDPRAR
                     IVPIAAFAPEEHWLVDRWW"
BASE COUNT          444 a          572 c          604 g          450 t
ORIGIN      
        1 gaaggcgcgg gagcgctcga ggtcgcccac cggcagggtc atgtggtcga tgcagatcgg
       61 acgcattcgc tcgaagatac gcgggcccgg ctcttcacag ctggagcgca ttaccagaag
      121 cttctggtaa tttggacgcg atgtcagagg agcttcgtca gaagggatat ctgagcccag
      181 agggggctgt ggcaggcgac ccagctgggc cgtttcaggc gttcaatctt ggggcctcga
      241 cgatcaacca gctagctcga gtccgcatcg tccctaggca cgattaccgg aagcggggga
      301 ttctcaaacc ggatggactg gtcgtttcag gggtggggtc tgcgccaact gtccacctcg
      361 tcgtcgagtt caaagatcga ggtgagctcg ataccgatgc caagctcgat cgggttctgg
      421 ccaaggttgc gactgagtac tgtgcgccac tcgattgtgc gctggcagtc gtaagtgatg
      481 gcgctgttgt gcgctgggtc gcggtcgacg ctggatctaa cacttggcgg atcatcaacc
      541 gcgatgacgg ctatccgctg gacgcattgg tcgatctctc ttcactggac ggacgggagg
      601 atgtagctcg aacgatctcg cgggtcacca gcgagctgaa tccggcgact ggagttctcg
      661 agcccgccga gacggttgac cccacgcgac ttgccgatca gacttggcaa gcgatctggc
      721 tggcatcggg agagaacccg gagcgctgcc tcgcctcgtt catcgagatc cttctcttca
      781 agtttctctc agacctcaag atcctgaccg aagatcgggc cggtcgcgcg actggctttg
      841 aacacgtagt cagccttcca ccctctgaag gcttggccta ctacttcgaa gtagtgcgac
      901 cggagatcat tcacctcttc ccaaaaggtg aggacggaac cactgtaatc ggcgggatcg
      961 tacttgagcc aaagaatctc gatcacggtc gcctatttac cgagatcctt cgtaagttcg
     1021 ataaggctgg cccgctcaag cggatcgacc cggaattcaa gtctcgcatc ttcgaacgat
     1081 tcctcaagaa aagcattagc cagaaaaact ggggtcaata tttcacgcct cgaaatgtcg
     1141 tcaaggccat ggttgagatg agcggcgtgg accgcatgtc tccgggcgaa gtggtggcag
     1201 atccgttctg cggggtgggt ggctttgtct tggagccgtt ggttcacaag cgaccgcatg
     1261 atttccgctc ttcgaaagac cgagccctct cctaccgagg gtacgaccgc gatgcgaaga
     1321 cgatcaccct ggcaaaggca aacatgctaa ttcacctttc ggaggttctc gagagggaac
     1381 ccgaaatcgc gcccgaaaga ctcgcgccag cgctgaatga gtcgtttcac gcgatggatg
     1441 aatctattac cggttccctg gaactagcac caaaggaaga gtgggacctt gtaatgacca
     1501 atccaccata tgtcgttacg ggcacgacta cgcaaagaaa aatgctctca gagaatccga
     1561 ttctcgctgc ctattactcc atctctggat ctggcgtgga gaatctctgt ctacaggaga
     1621 tcgttgcggg ccttaaggcc gggaagcgtg ccctcatgat cgtcccagat ggcctcctcc
     1681 ttcgccactc ggaggagtcg ttaaagaggc atcttctcag aacctgtttc ttagagtgcg
     1741 tgatatcgct gcccgtcgac acgttctact cgacccccaa gaagacttac atactcgtat
     1801 ttcgcaagaa gttggatgac cgcgaactcc agcaggacgg ggtcttgacg taccttgttg
     1861 gtgaggtcgg tgagactcgt gatgcaaagc gattcgtaac cacgaagaat cacctccctg
     1921 aagccgtatc gagcttcaag aagttccagg cggacccagg atctttctct ggagaccagc
     1981 cagtcgagag gcttgaggat ccccgtgctc ggatcgttcc tattgcggcg tttgcccccg
     2041 aggagcactg gcttgttgac cgatggtggc
//
LOCUS       DASTWE010000972         1437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_963410, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000972 DASTWE010000000
VERSION     DASTWE010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963410"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..807)
                     /locus_tag="VFT30_10120"
     CDS             complement(55..807)
                     /locus_tag="VFT30_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU5093033.1"
                     /translation="MPSVLLNKRIEFAAAHRYIRPEWNEAKNRTVFGRCYNPPAHGHN
                     YLLELTVSGEIDPKTGMVVNLFDLKRVLLNVIEEFDHKNLNLDMPYFKDRIPTSENFA
                     HVLWTKMAAQRDIGTLHTLRLCEDEDLYAEVTAADGQDMAAVAKRYSFNAVQEDHQGR
                     DWDCYVTVHGTIDLMTGMVTDISALDRLVRDRIIKPFDRQDLRKVLGSETVRGEALAK
                     TIWDRLAGHLSGGTLQNIHLVQTRDLSFDYAG"
     gene            complement(832..1416)
                     /locus_tag="VFT30_10125"
     CDS             complement(832..1416)
                     /locus_tag="VFT30_10125"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEU5093034.1"
                     /translation="MNVITANDLMSYEEYERRREVFRTKIIALKQRRRISVGPFITMV
                     FENRETLQFQIQEMIRAERIVEPAKVQDELDVYNALLPQQNELSATLLIEITDEAKMK
                     EKLDQFMGLDHGEKVAIIAGGEEAFGEFEGGRSHETKISAVHFVRFRPSAEMKRAFAD
                     LTRPVTIRVIHGGYHEKVPIPGSMREEWLADLRD"
BASE COUNT          294 a          403 c          377 g          363 t
ORIGIN      
        1 atgcggagat agaacgaaag tgctgttctt tgacacaata gagaggccat ccggttaccc
       61 cgcgtagtcg aacgagagat cgcgggtttg gacgaggtga atattttgaa gagttccgcc
      121 tgaaagatgg ccagcgagac gatcccagat ggtcttggcg agggcttccc ctctcaccgt
      181 ttcggagccg agcaccttgc gaagatcctg ccggtcgaat ggtttgatga tccggtctcg
      241 cactagtcga tccagcgcac tgatgtcggt caccatccct gtcatgagat caatcgtccc
      301 gtgaaccgtc acgtagcagt cccagtcccg cccctgatga tcctcctgga ccgcattgaa
      361 cgaatatcgc ttggcgactg cggccatatc ctggccgtcg gctgcggtga cctcggcata
      421 gagatcttcg tcctcacaga gccggagggt gtggagcgta ccgatatccc gttgagcagc
      481 catctttgtc cacagcacat gcgcgaaatt ttccgaggtc ggaatccggt ctttgaagta
      541 gggcatgtca agattcaggt tcttgtgatc aaattcttcg atcacgttca ggagcacccg
      601 cttgagatcg aacaggttga cgaccatacc ggtttttgga tcgatttcgc cggaaacggt
      661 gagctcaagc aggtagttat gtccgtgcgc gggagggttg tagcagcgcc caaacaccgt
      721 gcggttcttc gcttcattcc attccggccg tatatagcga tgggcggcgg cgaactcgat
      781 acgtttattc aacaaaacag acggcatggt tctcctgcgt cccgggccga gctagtctct
      841 taaatcagcg agccattctt ccctcatgct gccggggatc ggtacctttt cgtggtatcc
      901 gccatggatc actcgaattg taaccggtcg agttaggtct gcaaaggctc ttttcatctc
      961 ggcgctcggc cgaaatctga cgaaatggac tgcgctgatt ttcgtctcat ggcttcgacc
     1021 accttcgaac tcgccgaaag cttcttcccc tccggcaatg atcgccactt tctcgccgtg
     1081 atctagtccc atgaactgat cgagcttttc tttcatcttc gcctcatccg tgatctcgat
     1141 caagagggtg gcgctcaact cgttctgttg cggcagcagg gcattgtaca catccagctc
     1201 atcctggacc ttggccggtt cgacgattcg ttcagcccgg atcatttcct gaatctgaaa
     1261 ctgcagcgtt tcccggtttt caaacaccat ggtgataaac gggccgacag aaatgcgccg
     1321 ccgctgcttg agcgcgatga tcttcgtacg aaagacttct cgccgccgtt cgtactcttc
     1381 ataggacatg agatcgtttg ccgtgatgac attcaccgat tgttcctcaa tttgtca
//
LOCUS       DASTWE010000973         1657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1351310, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000973 DASTWE010000000
VERSION     DASTWE010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351310"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VFT30_10130"
     CDS             complement(<1..988)
                     /locus_tag="VFT30_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HEU5093035.1"
                     /translation="MKRNRICTGQWFVAVLFLAIGLSTYGCSDSVSVTENVEVPLSSL
                     TVTPGTLRPAFFSNTTSYTVNAPTSADNVTVTATPKDSTTTMTINGIPTATGQGRSVL
                     LGPPGSITSITIVLESLNGTESTYTVNVTRLLSSDNNLSALDVTPGNLNPPFDPDIED
                     YTANVGVLVDSVTVSATKSDPNASMRIGSVTVPPGTASGQATISLGAPGTPTPVSIEV
                     TAPNGSKKSYAITVNRLSDDNNLSALTVTPGALSPAFASNTLNYTADVGTGVTAVTVV
                     ATKSNPNAVLSGSIADPGAGQATGQATIPLGGPGTAAPVTITVTAPNGNSKTY"
     gene            complement(1258..>1657)
                     /locus_tag="VFT30_10135"
     CDS             complement(1258..>1657)
                     /locus_tag="VFT30_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HEU5093036.1"
                     /translation="LQNLIVSPGSLAPAFSAARTSYTVDVGSNVTSVTVLATLQDTNA
                     GLVVNGQGTGSGQARDISLGAEGSSTNITIIVTPPNGSAKTYTVTVNRASSGGDGNDG
                     GNDGGKGNNSGKKNNDGNKGGDRDDGDDDD"
BASE COUNT          318 a          419 c          495 g          425 t
ORIGIN      
        1 tgtaggtctt gctgtttcca tttggggcgg tcacggtgat tgtcacagga gcggctgttc
       61 ctggcccacc gagcggaata gttgcttgac cggttgcttg ccctgctccg gggtcagcga
      121 tcgatccaga tagaacagca ttcggattgg acttggtggc aacgacagtc accgctgtaa
      181 cccccgtgcc aacgtcggcg gtgtagttca aagtgttcga tgcaaaggcg ggcgacaagg
      241 cgcctggcgt taccgtcaac gccgataaat tgttatcgtc agagagacgg ttcacggtaa
      301 tggcgtagga ctttttactg ccattcgggg cggtcacttc gatcgacaca ggggtaggtg
      361 ttcccggtgc accgagtgaa atggttgctt gtcctgaagc tgttccaggc gggacagtca
      421 cggagccgat ccgcatactg gcattcggat cagacttggt ggcggagacg gtgacactgt
      481 cgacgaggac gccgacattg gccgtgtaat cttcgatatc cggatcaaag gggggattca
      541 agttgcctgg tgtcacatcc aaggctgaca gattgttgtc gcttgataag agtctcgtga
      601 cattgacggt ataggtgctc tccgttccat ttagcgattc caagactatg gtaatgctcg
      661 tgatcgatcc cggaggtcct aacagcacgg atcgtccctg tcctgtagcg gttgggatcc
      721 cgttgatcgt catggttgtc gtgctgtctt tgggggtggc tgtcacagtg acgttgtccg
      781 cggatgtcgg cgcattgacc gtatagctcg tggtattgct gaaaaacgca ggccgcaggg
      841 taccgggagt gaccgtcaag ctggagaggg gcacttctac gttttccgta accgatacgg
      901 agtccgagca gccataggta ctcaagccga ttgcgaggaa aaggacggcg acgaaccatt
      961 gtccagtgca gatacgattc cgtttcatgt aaagcctgtc ttttctttat gtgcgagtga
     1021 ggaaccgttc attcttatga accctaactg tagatgccga tactacgaga gggttcttga
     1081 tcggtctatc cctacctgtg taagggcgag caggtgctgg gaaggtgtgc tcccactttt
     1141 cggtgggtca tggttcgggt ccggcgcctg agcaggtatg cctgtgagct aaaaatatgt
     1201 gcgaggggag gatttagagg gaagaccgtc tttatggcgc ggtctcatca ttgccggtca
     1261 atcgtcatca tctccgtcgt ctcggtcacc tcctttattc ccatcattat ttttcttgcc
     1321 gctgttgttt cctttgccac cgtcgtttcc accatcgttg ccgtcgcctc cgctggacgc
     1381 tcggttgact gtgacggtgt aggtcttggc gcttccattc ggtggcgtga cgataatggt
     1441 aatgttcgta ctcgaccctt cagctcctaa tgagatgtca cgtgcctggc ccgagccggt
     1501 tccttgtcca ttcaccacca ggccggcgtt cgtatcctga agagtggcga gcacggtgac
     1561 gctcgtcacg ttgctaccga cgtccaccgt ataactcgtt ctcgccgcag agaacgcagg
     1621 agctaatgag ccgggcgaca cgatcaggtt ttgcaag
//
LOCUS       DASTWE010000974         1254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_155569, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000974 DASTWE010000000
VERSION     DASTWE010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155569"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..>1254)
                     /locus_tag="VFT30_10140"
     CDS             complement(19..>1254)
                     /locus_tag="VFT30_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5093037.1"
                     /translation="KQTVLLSFDTLRANKFRSFLTILGVVIGVTSVIAVASIIQGLNN
                     VVQDRVAALGTKVFFVSRIPPFTFGRPPERIRKRKFLYMEDAMAIREQCPSVEDATPF
                     QQRAVFTGQPNLVRYGNERVESAVLRSAEPQYIQVLPIFSMRDGRFISDFDNEHSAKI
                     CAIGSGIADTLFPATDPVNKEINLNGQIFRVIGVFERDPGLFGGPGIDQFVMIPYNTF
                     HKLYPEIEDNLLLVSVRDPSLLSKAQDEVTDVLRRRRRVRTQDESDFDLMSPDILSQV
                     WNQLTGAIVILTMVISSIGLLVGGIGVMNIMLVSVTERTREIGIRKAVGARRRDILTQ
                     FLIEAMTLTGTGGIIGILIGGLISLAIRTFLPALPSEVSLIWVVAAFSVSVGVGLFFG
                     IYPAKRAAELDPIVSLRYE"
BASE COUNT          318 a          352 c          326 g          258 t
ORIGIN      
        1 tgtcggacgc cggttcccct actcataccg caaggacaca atgggatcga gttcggcagc
       61 acgcttggct gggtagatcc cgaaaaacag gccaacgccc acggacactg agaaagccgc
      121 aaccacccag atcagcgaaa cttctgaggg cagggcagga agaaaggttc gaatcgccag
      181 gctgattaag cctccaatca aaatgcctat gattcctccg gtgccggtaa gtgtcatggc
      241 ttcgatcaaa aactgcgtca gaatatcgcg gcgtctggct cccacggctt tacggattcc
      301 gatctcacga gtccgctctg tcaccgaaac cagcatgata ttcatcacgc cgatgcctcc
      361 caccagcagg cctatggaag aaatcaccat cgtgaggatg acaatcgccc ccgtcaactg
      421 gttccagact tgagacagga tgtcgggcga catcaaatca aagtcgctct catcttgtgt
      481 ccgcactcgc cgacggcggc ggaggacgtc cgtaacttcg tcttgagcct tggaaagaag
      541 cgatggatcg cggacgctta ccagaagcag attgtcctca atctccgggt agagtttatg
      601 aaaggtgttg tagggaatca tgacaaactg gtcgattccc gggcctccga acaagccagg
      661 atccctttca aaaaccccga tgacacgaaa tatttgtccg ttcaggttga tttccttgtt
      721 gacaggatcc gtcgccggaa acaaggtgtc ggcgatgccc gatccaatgg cgcagatttt
      781 cgcggaatgt tcgttgtcaa aatcgctaat gaatcttcca tcccgcatgg agaatatcgg
      841 caacacttga atgtattgcg gctcggcgct gcgcaacacg gcgctctcta ccctttcatt
      901 gccataacgc acgagattgg gctgtcccgt gaagactgca cgttgctgaa aaggtgtcgc
      961 atcctcgacc gacgggcact gctcacgaat ggccatggca tcctccatgt aaagaaactt
     1021 gcgcttgcgg atccgttccg gtggccggcc aaaagtaaat ggaggaatcc gggagacaaa
     1081 gaaaactttg gtccccaaag cggcgacccg atcctgaacg acattgttca gaccttgaat
     1141 gatggaggca acggcgatca ctgacgtcac tccgatcacc acccccagga tggtcagaaa
     1201 cgaacgaaac ttgttggctc gcagcgtgtc gaacgaaagc aacaccgtct gctt
//
LOCUS       DASTWE010000975         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_607477, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000975 DASTWE010000000
VERSION     DASTWE010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607477"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFT30_10145"
     CDS             complement(<1..374)
                     /locus_tag="VFT30_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093038.1"
                     /translation="MTESRDRRLADRALAAQYALADIQEGNPWVDMVGSVLYAGGQKE
                     GQRLTAATKRIASEASTFVVSTDMTEVVKQAAEDLMFLWMQEGEVPDVILPYDLPHPQ
                     GFVLFSHSINLGHFLDDYEDEVR"
     gene            complement(374..682)
                     /locus_tag="VFT30_10150"
     CDS             complement(374..682)
                     /locus_tag="VFT30_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093039.1"
                     /translation="MPVPTVGPVKHSKALTLEQLVPGTQVIEVNVEDRRLVTIDRIED
                     ESDSDARIFFVFKTTPRQQRLHGWGSTRTTSVRATDYGAIPSPCDGQWNLVNRFEQNV
                     "
     gene            complement(685..933)
                     /locus_tag="VFT30_10155"
     CDS             complement(685..933)
                     /locus_tag="VFT30_10155"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEU5093040.1"
                     /translation="MAENEFTVTNSTNISQVSFTHEHPGEGDGALVVVFTSGRAYRYS
                     GVPMDVFTTLKDVHEAGGSVGRAFAQHVRSAGFEYEEV"
     gene            complement(975..>2321)
                     /locus_tag="VFT30_10160"
     CDS             complement(975..>2321)
                     /locus_tag="VFT30_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEU5093041.1"
                     /translation="PFNEATLTAFTDIAGGRVFEPFFSVGSVSSYITGATGPESGIRI
                     KVSGSFGATLSAESETALTSALAASGEFTIETWTSASNLVFFSTERMSLKRDGGRILC
                     VSWFDSEVYAESFFATYVLIPEHMMSGMHHWVFVFDGENAKFYADGHLLRSVEAPAPV
                     WDDIGDFSTSITIGASVAGGGTVSLDDLAVYDRALPESEVQAHCAERATVYDPPSDPI
                     DAYHESVQNSYPLCYVPAITGQVPQDIEGACSIDFDAAPKVVGRTFLFQEFNVIVQPG
                     QTMWEHGYRSYEMAIFGEHVVEFGHTFMSNLSGFGLRWYAFGTTPPSVNIKYLPSSGS
                     EIDVLGSWADDAWHHVVVNLVLREHPSSTNGLWLYVDGDEVLALDLSSGTIPPYNPLT
                     QWPQFRGTPRFFDRAAVYSHILTEEEITERAAMMANLPVPFGADGDRLPSVGRLLI"
BASE COUNT          491 a          687 c          690 g          453 t
ORIGIN      
        1 cgcacctcgt cttcgtagtc atcgaggaag tggccgaggt tgatggagtg ggagaagagc
       61 acgaacccct gtgggtgcgg gagatcgtac gggaggatca cgtctggaac ttcaccttcc
      121 tgcatccaca agaacatgag atcttcggca gcctgcttga ccacctcggt catgtccgtg
      181 ctgacgacga aggtcgaagc ttcggatgcg atcctcttcg ttgcagccgt gagtcgttgc
      241 ccttccttct gaccacccgc gtagagcaca ctccccacca tgtcgaccca cggattgccc
      301 tcttggatgt cagccagtgc gtactgagca gccagagccc tgtcggccag acgacggtcc
      361 cgagattcag tcatcagacg ttctgctcga agcggttcac cagattccac tgcccgtcgc
      421 aaggcgaagg aatcgctccg tagtccgtcg cccgcactga agttgtacga gtggagcccc
      481 atccgtggag ccgttgctgt ctcggagtgg ttttgaagac gaagaagatg cgagcatcgc
      541 tgtcgctctc gtcttcgatc ctgtcgatcg tgacgagacg acgatcttcg acgttcacct
      601 cgatgacctg cgtcccaggc acgagttgtt cgagagtgag ggccttggag tgcttgacag
      661 gacccacagt cggcaccggc acgatcagac ctcctcgtac tcgaagccgg cactgcggac
      721 gtgctgtgcg aaggcacgac ccaccgagcc gccggcctcg tgcacgtcct tgagggtggt
      781 gaagacgtcc atggggacac ctgagtagcg gtaggcacgt ccgctggtga agacgacgac
      841 gagggcacca tcgccctcac ctgggtgctc gtgggtgaag gacacctggg agatgttggt
      901 gctgttggtg acggtgaact cgttctcggc catgcctgac agagtagttc aatttcttcg
      961 gattgacaac cctctcagat caacagacgt cccactgacg gaagccggtc accatcggct
     1021 ccgaacggga cgggaagatt cgccatcatc gctgctcgct ctgtgatctc ttcttccgtg
     1081 aggatgtgcg agtacaccgc agcacgatcg aagaacctcg gggtaccacg gaactgaggc
     1141 cattgggtga gggggttgta cggagggata gttcccgagg acagatcgag agccagcacc
     1201 tcgtcaccat cgacgtagag ccagagcccg ttcgtgctcg acggatgctc gcgcaacacc
     1261 aggttcacga cgacgtggtg ccatgcatca tcagcccacg atccgagaac gtcgatctcc
     1321 gatcccgacg agggcaagta cttgatgttg accgaaggag gtgtcgttcc gaaggcgtac
     1381 cagcgcaatc cgaaacccga caggttactc atgaaagtgt gaccgaactc cacgacgtgc
     1441 tctccgaaga tcgccatctc gtagctgcgg tagccgtgct cccacatggt ctggcccggc
     1501 tggacgatca cgttgaactc ttggaagaga aaagttcttc ccacgacctt cggagcagca
     1561 tcgaagtcga tgctgcacgc tccctcgatg tcttgcggaa cctgaccggt gatggccggc
     1621 acgtagcaga gcgggtagga gttctgaacg ctctcgtggt aggcatcgat cggatccgaa
     1681 ggagggtcgt acacagtggc cctctcggcg cagtgcgctt ggacctctga ctccggcagg
     1741 gccctgtcgt agacagccag gtcatcgagt gacacagtgc caccacccgc tacggatgcg
     1801 ccgatagtga tgctcgtgga aaagtcaccg atgtcatccc acacaggtgc aggagcttcg
     1861 accgaccgaa gaagatgacc atctgcgtag aacttcgcat tctcaccatc gaacacgaag
     1921 acccagtggt gcatcccgct catcatgtgc tcagggatca acacgtaggt ggcgaagaag
     1981 gactcggcat acacctcact atcgaaccag ctgacgcaca agatcctgcc gccatctctc
     2041 ttcaacgaca tgcgctcggt gctgaagaag acgaggttgc tcgctgatgt ccaggtctcg
     2101 atcgtgaact cacccgatgc ggccagagcc gacgtgagag ctgtttcgga ttcggctgag
     2161 agggtggcgc cgaacgagcc ggagaccttg atccgaatac ccgactccgg tcccgttgcc
     2221 cccgtgatgt aggacgagac cgatcccacc gagaagaagg gctcgaaaac tctccctcct
     2281 gcgatgtctg tgaacgcagt gagcgtagcc tcgttgaagg g
//
LOCUS       DASTWE010000976         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_26432, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000976 DASTWE010000000
VERSION     DASTWE010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26432"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VFT30_10165"
     CDS             <1..149
                     /locus_tag="VFT30_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU5093042.1"
                     /translation="LVHEQNFHTREEAQVAVFEFIEVFYNRQRLHQTLGYVSPIQFET
                     APVP"
     gene            277..>1520
                     /locus_tag="VFT30_10170"
     CDS             277..>1520
                     /locus_tag="VFT30_10170"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093043.1"
                     /translation="MTTDEISPLPPRSETLYADVIVPRHIAKAFTYRVPPTLAQTIAI
                     GHPVLVPFGRTVLEGIIISLSNHLATEIKSASIREIHSLDHDGQASTLPPALFELSRK
                     IAEYYVAPWGQCLRLILPSMATQKISCARYVATAQGRLALEAGLCPDYLKPILERIAR
                     RTPGILSSTLQPTRQGNAMRIDALINKSWIALMPSNNANIELQKPCRRRTLDEQDRPS
                     VDGIRPTETLPEVDSSWKTRVAQCLHSNHMRKLVLYAPWEHRLSRLVDAIQQAHSMKR
                     SIIVLTGEIVRASWLKQVLSRFTNLQIILARPSSGLWQASEGQIPSVVVGTRSAVFLP
                     LKSIGLIWVEGEEDPALKEPQEPRYHAREVAHLRAESERALVVLASAHPSLESRFDTE
                     AEIHHVPQEIALQPTIELVDLR"
BASE COUNT          354 a          435 c          381 g          350 t
ORIGIN      
        1 agctcgtgca tgaacagaat ttccacaccc gcgaggaggc gcaagtggcg gtgtttgagt
       61 tcatcgaggt gttctacaat cggcagcgac tgcatcagac gctgggctac gttagtccga
      121 tacagtttga aactgcacct gtaccgtaac tcggtgtcca tgaaatcagg ggtagctcag
      181 attacttttc tggtataggt ttctcaccgg ctcaccttcc taaccgaaca ccgatgcgac
      241 cgtgtccttc gcatttccag actgctagaa tgcgacatga ctaccgatga gatctcgccc
      301 cttcctcctc gatcggagac actctatgca gacgttattg tgccccgcca tatcgcgaag
      361 gccttcactt atcgcgtccc tcccacctta gctcagacca tagcaatagg gcatcccgtc
      421 cttgtgccgt ttggacgtac ggtgctggaa gggattatca tctcgctgag taatcatctt
      481 gcgaccgaga taaagtctgc ttccattaga gaaatccact ctctagatca tgatgggcaa
      541 gcttctacac tgccgccggc gctgttcgaa ctatctcgta agatcgcaga atactacgtt
      601 gcaccttggg gtcagtgtct tcggctcata ttgccctcga tggcaaccca aaaaatctcc
      661 tgcgcgcgat acgtggccac tgcacaaggt cgtttggcct tggaagcggg cctttgtcca
      721 gattatctga agcctattct cgagcgaatt gccagacgaa ctccgggaat cctgtcctcg
      781 acacttcagc caactcgaca gggaaatgcc atgcggatcg acgccctcat caataagtcg
      841 tggatagcgc tcatgccttc gaacaatgcc aacatcgagc tccaaaagcc gtgtagaagg
      901 cgcaccttgg acgagcaaga tcgcccgtct gtggacggca ttcggcccac agaaacgctc
      961 ccggaggtcg actcctcatg gaaaacgcgc gtcgcgcagt gtcttcatag caaccacatg
     1021 aggaaactcg tactttatgc cccgtgggaa caccgactca gccggcttgt cgacgccatt
     1081 cagcaggcgc actcaatgaa gagatccata atagttctca ccggcgagat cgtgagggca
     1141 tcgtggttga agcaagtgct ctcaagattc acgaacctcc agattatcct cgcacgccca
     1201 tcttcaggtc tatggcaagc gagtgaggga cagattccct cggtagtcgt aggaactcgc
     1261 tcagccgttt ttttgcctct taaatcgatc gggttgatct gggtcgaagg agaagaggat
     1321 cccgccctga aagaacccca ggagcctcgt taccatgcca gggaggtggc tcatttgaga
     1381 gctgagagcg aacgggcgct cgttgtactg gcatcggctc acccatccct tgaatcaagg
     1441 ttcgataccg aggctgagat ccatcacgtg ccacaagaaa ttgctcttca acctacgatt
     1501 gagctcgttg accttcgcaa
//
LOCUS       DASTWE010000977         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_123202, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000977 DASTWE010000000
VERSION     DASTWE010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123202"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFT30_10175"
     CDS             complement(<1..348)
                     /locus_tag="VFT30_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5093044.1"
                     /translation="MATILVIDDEQSIRGLLKEVLVKAGHRVLEAEDGRKGLTLYQKE
                     PVDLVIMDLLMPDTDGLEATLQLTREYLDAKVIAITGAQGDHNFLDVAKLFGARRAFE
                     KPFDINKLLEAVNE"
     gene            699..1274
                     /locus_tag="VFT30_10180"
     CDS             699..1274
                     /locus_tag="VFT30_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEU5093045.1"
                     /translation="MNTTEDQSKWAWTRRAFLQVSLTGTLLLSGRLVGPQPVQARELP
                     EGRLTLVNVWTNERLDITYRDEDGSYDLAALDDVNYLLRCHYTGEVGAIDVRVLEHVN
                     LVQKKLGAQEEIHVISGFRSPEYNAMLVRTDRRVARNSLHMQGQAIDLLIPGISPAKL
                     RQAALELRYGGVGFYKRSSYVHLDSGPFRHW"
     gene            complement(1364..3586)
                     /gene="glgB"
                     /locus_tag="VFT30_10185"
     CDS             complement(1364..3586)
                     /gene="glgB"
                     /locus_tag="VFT30_10185"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU5093046.1"
                     /translation="MPLELTIQQDDLDRLIAGTHWSPHSILGPHATTIDGRTFLAIRA
                     WLPQIKDIEVISDSSLWRMTRICEEGLYEALLPDTTQTPFYKLRVTYHDDTVAEIHDP
                     YAFPPLLTDYELHLFAEGTLYKAYDSFGAHIRTFEGVCGVHFVVWAPNAVRVSIIGDF
                     NGWNGLCHPMTSRGSTGLWELFIPNLPEGTLYKFEIRSREHGALLKKADPYAVASELR
                     PRTASIVRDLSTYPWGDGAWMSARSEWDPLTASLSIYEVHLGSWMRVPEENNRWLTYE
                     ELAAKLIPYATDLGYTHLELMPVSEHPFDGSWGYQATGYFAATSRYGDPKGFMAFVDA
                     AHQAGLGVIMDWAPAHFPDDPHGLAMFDGTHLYDHADPRLGYHPDWHSRIFNYDRVEV
                     RTFLLNSALFWLDKYHIDGLRVDAVASMLYLDYGRKSGEWIPNEFGGHENLGAVSLLK
                     ELNVLIHREFPGAVTIAEESTAWPGVSRPTYTGGLGFTFKWNMGWMHDMLAFFQHDPI
                     YRRFHQNQITFGLLYAFSENFILALSHDEVVHGKRTLLDKMPGDVWQRFANLRLLYGY
                     MYSHPGKKMLFMGGEFGQWREWNHDASLEWHLCKEEPHRGLQRLIHDLNRIYREEPAL
                     HEVDFDWSGFQWIDFSDADNSVIDYLRKTKSTQAAIICLCNFTPIPRHGYHIGVPEPG
                     WYRELMNTDGIAYGGSNMGNGGGVHAAETPSHGFRYSLTVTLPPLSILWLKQPPTNER
                     "
     gene            3709..>4184
                     /locus_tag="VFT30_10190"
     CDS             3709..>4184
                     /locus_tag="VFT30_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5093047.1"
                     /translation="MALQGDPSPFGTKDIMSSLMLALLIFGLCYVLIMTERIHKTIVA
                     MFGAAVMIGLGVVSQEEAFYSHEFGVDYNVVFLLIGMMVIINIVRETGLFEELAVWAA
                     QRADAKPFRLLVLLAILTAVLSAMLDNVTTVLLMAPVTLAITKRLELNPVTFLMTEA"
BASE COUNT          941 a         1068 c         1169 g         1006 t
ORIGIN      
        1 ttcattcacc gcttcaagga gcttgttgat gtcgaacggc ttttcaaagg cgcgacgcgc
       61 cccgaagagt ttggcgacgt ccagaaagtt gtggtcgcct tgcgctcctg tgatagcgat
      121 cactttggcg tcgagatatt cgcgggtgag ttgaagagtt gcctcaaggc cgtctgtatc
      181 cggcatcaac aagtccataa tcaccaggtc gaccggctct ttctggtaga gcgtgagtcc
      241 tttgcgtcca tcctccgctt caagcacgcg gtgtcctgcc ttgacaagga cctctttcag
      301 cagccctcgg atggattgtt cgtcgtcaat gactaggata gtagccatac atgctctact
      361 gattttttct gatcttacct ggtgctcgcg atacagtcta gaaagaaaat gatttctgga
      421 ataggtgcaa gaggtgaaat gaagatgagt ccgattggcc tcatgtgttc ctgacaactg
      481 caagccctga tgtgacatcg catcttaatc tttatattat gaaggtcaat gcatacatcg
      541 tttaatgtga aagtggatgc ataaacatac aatctaaggc gtgaagaggt atttagcatc
      601 ctctagggac aaaactgttt gacccaattt gggatttttg ttacaaggcc aaaagttctt
      661 tcaatcatca ctgtccattc tctggaggag ctgtttcagt gaacactaca gaagatcagt
      721 ccaaatgggc ctggacccgg cgagcgtttc ttcaggtttc cctgacgggg actctattgc
      781 tgagtggacg attggttggt ccacagccgg tgcaggctcg tgagcttcca gaaggcagac
      841 tgacgttggt aaacgtgtgg accaatgagc ggttggacat cacctaccga gatgaagacg
      901 ggtcctatga ccttgcggca ctcgatgatg tgaattacct tctgcgttgc cattacacgg
      961 gtgaagttgg ggcgatcgat gtacgtgtgc tggagcacgt gaatttggtg cagaagaagc
     1021 ttggcgctca ggaagaaatc catgtgattt ccggctttcg gtctcccgag tacaatgcca
     1081 tgttggtgcg gacagaccga cgtgttgcca ggaacagctt gcatatgcag ggacaggcga
     1141 tcgatcttct cattcctggt atttctcctg cgaagcttcg ccaagccgcc ctcgaactgc
     1201 ggtacggcgg ggttggcttt tacaaacgct cgagctacgt gcatttggat tccggcccct
     1261 tccgacattg gtagctctcc cgcttctgtg cagacgatct ttcatctttc gaacacatcg
     1321 gcttctgaca aggatcggga cagtgttgtg tcggaggcgg atactaacgc tcgttggtcg
     1381 gcggttgttt tagccagagg atggataggg gtgggagggt gaccgtcaga gagtaacgga
     1441 acccgtggct gggcgtttcc gctgcatgaa caccgccgcc attccccatg ttgctgccgc
     1501 cgtaggcgat cccgtcggtg ttcatcaact cccgatacca acccggttct ggaacgccaa
     1561 tgtggtaacc gtggcggggt atgggcgtga agttacacag gcagataatg gctgcttgcg
     1621 tactttttgt ctttcgaagg tagtcaatga cggagttgtc ggcgtcgctg aagtcgatcc
     1681 actgaaatcc gctccagtca aagtcgacct catgcaatgc cggttcttcg cgatagatgc
     1741 gattcaagtc gtggatcaga cgttgcaagc cacgatgcgg ctcctctttg cagagatgcc
     1801 actctaagct ggcatcatga ttccattccc gccactggcc gaactctcca cccatgaaga
     1861 gcattttctt gccgggatgg ctatacatat agccataaag tagccggagg tttgcaaacc
     1921 gctgccatac atcgccgggc attttgtcca gcaaggttcg tttaccgtgg accacttcgt
     1981 catgagagag ggcgaggata aaattctcgc tgaaggcata gagcagaccg aacgtaattt
     2041 ggttttgatg gaaccgacgg tagatcggat cgtgctggaa aaaggccagc atgtcgtgca
     2101 tccatcccat attccacttg aacgtaaagc cgagcccgcc cgtataggtc ggccgagaca
     2161 caccgggcca ggccgtggac tcctccgcga tcgtgacggc cccggggaat tctcggtgga
     2221 tgagtacgtt gagttctttc aagagtgaga ccgctccaag attttcatga ccgccgaact
     2281 cattcggaat ccattcaccg gattttcgac cgtagtcgag gtagagcatc gacgccacgg
     2341 cgtctacacg caatccatcg atgtggtact tgtccaacca gaagagcgcg ctgttcaaca
     2401 ggaaggttcg cacctcgact cggtcataat tgaaaatccg gctgtgccaa tccggatgat
     2461 agccaagacg cggatcggca tggtcataaa gatgtgtgcc gtcgaacatc gccaatccat
     2521 ggggatcgtc tggaaagtgc gcgggagccc agtccatgat cacgccgaga ccggcttggt
     2581 gagcggcgtc cacgaacgcc atgaacccct tgggatctcc atatcggctg gtggctgcaa
     2641 agtatcccgt cgcttgatac ccccatgatc catcgaacgg gtgttccgac accggcatga
     2701 gttcgaggtg cgtgtagcct aggtccgttg cgtaggggat cagcttcgca gcgagttctt
     2761 cataggtgag ccaccgattg ttttcctccg gaactcgcat ccatgacccg agatggactt
     2821 cataaatgga cagggaagcg gtcaacggat cccattctga tcgagccgac atccacgcgc
     2881 cgtcgcccca gggataagtg gataagtcac gcacgatcga ggctgtccgt gggcgaagct
     2941 cgctcgcgac ggcgtagggg tcggcttttt tcagcaaggc gccatgttct cttgatcgaa
     3001 tctcgaattt gtagagagtg ccttccggca aattgggaat aaagagttcc cacagaccgg
     3061 tagatccccg actcgtcatg ggatgacaga gaccattcca tccattgaaa tcgccaatga
     3121 tgctcacgcg aacagcattg ggagcccaca ccacaaaatg aactccgcag acgccctcaa
     3181 aggtgcggat atgggcgccg aaactgtcgt aggccttgta taaggttcct tcggcaaaga
     3241 ggtgcagctc ataatccgtc aacagtgggg gaaatgcata cggatcatgg atttcggcga
     3301 cggtgtcgtc gtggtatgtg acgcgaagtt tatagaatgg tgtctgggtt gtatccggaa
     3361 gaagcgcctc atacagcccc tcctcacaaa tacgagtcat acgccagagg ctggagtcgg
     3421 atatgacttc tatatccttg atctggggga gccaggcccg gatcgccagg aaagtgcggc
     3481 cgtcgatcgt cgtggcatgt gggcccagaa tcgagtgagg ggaccagtgt gtgcctgcaa
     3541 tgaggcgatc gaggtcatcc tgttgaatgg tcagctcaag aggcatcaca ttatcctaat
     3601 ggctacagcc ttgattagga aggagaaaag ttctccatgg acgacgtcac atgacatgcc
     3661 agaaagacaa ctctgattgc attgtcgcga tcggacccgg ataatgcgat ggctcttcaa
     3721 ggtgatccat ctccgttcgg taccaaggac atcatgtcat ctctgatgct ggcccttctt
     3781 atcttcggcc tgtgctatgt gctcatcatg accgaacgga tccacaagac aattgtggcc
     3841 atgttcggcg cggccgtgat gatcgggctc ggagtggtct cgcaagaaga ggcgttttat
     3901 tcccatgagt ttggggtgga ctacaacgtc gtcttcttgc tgatcggcat gatggtgatc
     3961 atcaatatcg tgcgggagac cgggctgttc gaggagttag ccgtttgggc ggcacaaaga
     4021 gccgatgcga aacccttccg attactcgtg ctgttagcca tcttgacggc tgtattgtcc
     4081 gccatgctcg ataatgtcac caccgtcctg ctcatggccc cggtcaccct tgcgatcacc
     4141 aagcggttag agttgaaccc tgtcacattt ctgatgaccg aggc
//
LOCUS       DASTWE010000978         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_58583, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000978 DASTWE010000000
VERSION     DASTWE010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58583"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1034
                     /locus_tag="VFT30_10195"
     CDS             33..1034
                     /locus_tag="VFT30_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5093048.1"
                     /translation="MLRLGVIGYGYWGPNVVRNFLGHHDCKVITICDKSSAALTRVRS
                     AHPGVGVTIDPDAVLLATDIDAVAIVTPVSYHYELAKKALENGKHVFVEKPFTATSAE
                     AEDLIELADRRGLQIMVDHTFLFTGVVRKIKQLIDEGTLGRLYYYDSTRVNLGLFQND
                     VNVLWDLAPHDLSIMDYLIGVEPELVVATGGAHVNNLENIAYLTVYFPDNVLAHINVN
                     WLSPVKMRTTLVGGQKRMLVWNDLDLAEKLRIYDKGADVKNESGMHAALVSYRTGDMH
                     APKIEEAEALKVETRYFLDCIMDGTKPINDGTAGLRVVRILEAAEQSLNQHKEIMLA"
     gene            1053..>1671
                     /locus_tag="VFT30_10200"
     CDS             1053..>1671
                     /locus_tag="VFT30_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU5093049.1"
                     /translation="MIITHIEGDRATTRPTILEETFFKQTITRERKRTERSGMSMVLL
                     LIGFPIGLGESPASWVAVMEALSAVQSDSDLLGWFESRDTIGLIVPEIKSADLMSTCD
                     RLESTFRNAIALQGDKELAQRFPIRVRVYPEPTTSNEMQVSPTDPLLYPEFSTNVSAA
                     PNFQTLKRGMDIIFSALLLILLLPMFTLIAVLVKLSSSGPVFFKQT"
BASE COUNT          377 a          430 c          480 g          384 t
ORIGIN      
        1 aaggtctgct acctctttca aggagggcca ttatgctacg actcggtgtg atcgggtatg
       61 ggtattgggg gccgaacgtg gttcggaact ttctcggaca tcacgactgc aaggtcatca
      121 cgatctgcga taagtcttcc gccgcattga ctcgtgtgag gtcggcgcat cccggcgtcg
      181 gtgtgacgat tgatccggat gccgtgcttc tcgccacgga cattgatgcg gtggcgattg
      241 tgacgccagt gtcataccac tacgaattag ccaagaaagc gctggagaat gggaagcacg
      301 tattcgtgga aaagcctttt accgcgacat ccgctgaagc cgaggacctc atcgaactgg
      361 cggatcgccg tgggctccag atcatggtgg accatacctt tctttttacc ggcgttgtcc
      421 gcaagatcaa gcaactcatc gatgaaggca cattgggccg cctctactac tatgattcga
      481 cgcgcgtgaa tttggggttg tttcagaatg acgtgaacgt gctctgggat ctcgcgccgc
      541 atgacttgtc cattatggat tacctcatcg gggtcgaacc tgagttggtg gtggccaccg
      601 gcggagccca tgtgaataat ctcgaaaaca tcgcctacct caccgtgtat ttccctgaca
      661 atgtcctggc acacatcaac gtcaattggc tctcgcccgt gaaaatgcgt acgacgctgg
      721 tcggtggaca aaaacgaatg ctggtttgga atgacctcga cctggcggaa aagctccgca
      781 tctatgacaa gggcgctgat gtcaagaatg aatcggggat gcatgcggcc ttggtcagct
      841 acaggaccgg cgatatgcac gcgccaaaaa tcgaagaagc tgaagcgttg aaggtggaaa
      901 cccgctattt tctggactgc atcatggacg gcacgaaacc gatcaacgat gggacggcgg
      961 ggttgcgagt cgtgcgtatt ctggaggcag ccgaacagtc gctcaaccag cataaagaaa
     1021 ttatgctggc ctagccgatc gaagggagca gcatgatcat tacacatatc gaaggagatc
     1081 gggcgactac gcgcccgact atcctcgaag agacattctt caaacagaca atcaccaggg
     1141 aacgcaaacg gacagagcgt tccgggatgt cgatggtgct gttgttgatc ggctttccga
     1201 tcggtttggg tgagtcaccg gcgtcatggg ttgccgtgat ggaggccctg tcggccgtac
     1261 aatcggacag tgacctgctc ggctggttcg agtcgcggga tacaatcggt ctgatcgtgc
     1321 ccgagattaa gtccgccgac ctgatgagta cctgcgatcg acttgaaagc acattcagga
     1381 acgcgattgc gcttcagggc gataaggaac ttgcgcaacg ttttccgatc agggtgcgtg
     1441 tgtatccgga gccgactaca tcgaacgaga tgcaggtgtc tcctacggat ccactgctgt
     1501 atccggaatt ctcgacgaac gtgtcggctg ccccgaactt tcagacgctc aagcgaggca
     1561 tggatattat tttcagtgca ctactgttaa tcctattgtt gccgatgttc accctcattg
     1621 ccgtacttgt caagctgtcg tcgtccggcc cggtcttttt caagcagacg c
//
LOCUS       DASTWE010000979         1280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_705093, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000979 DASTWE010000000
VERSION     DASTWE010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705093"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            689..>1280
                     /locus_tag="VFT30_10205"
     CDS             689..>1280
                     /locus_tag="VFT30_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5093050.1"
                     /translation="MSHGSTTSTILIVDHNPAALSMKRRLLSRESYQIIEAENGTDAL
                     MLAAAELPDLVLLDANLPDIDSFDACRQLKTQSETKFMKILQTSAARMSAPDQPRNLD
                     VGPDAYLVEPLEEEELIGIVRALLKLAQQERDNLQLAEKLSLVERQLSAATETVQCTE
                     LKQTEQTLRESEERFAKAFRASPHPIGITEVATGRCI"
BASE COUNT          301 a          376 c          336 g          267 t
ORIGIN      
        1 tagccaagac ggtcggggcc gatctcaaaa cattgctgaa tgaggaattg gtccggatta
       61 agacggttct cgaggaagat gtcatccctc gggatgccca acgccgcgaa tccgaatcat
      121 caccgatgct gaaggcggaa cagttggagg gataggactc agtcgacaga gcgatgctcg
      181 ctgagtgtgt tccctcggaa cagagtcacc ttcatggcca ttcgatttca tcgtcatggg
      241 ggaggtcgag ataccgcgca gtggaatagt atatgacagc tagccggggc ttgcggccgg
      301 tccgcgcggc gatccgcggt ttgttgtaat tttccaacac ccttgaaagt aacaagacgg
      361 caagagtaga ttcggcggat tgccgcggaa ctgttcatct taccaagtac cagtccacgt
      421 tcccttcgag cctctccacg caagggtccc ccagccagaa ccctcccatt ctggtcgatg
      481 acgcatatcg ttgcagtagt cttcccccta ggactctccc tagtttccat attgtggcct
      541 ttatctatac tgcggcgcgc agtaagctgc gacctcactt cattcgatat ccgaaggcat
      601 gccggaatag tttcgcttca cttaagacac ccgattttgt tcgcctctct tcgtatgagt
      661 caaggcgcta cggatcagaa ggaaattcat gagccacggc tcgacaacct cgaccattct
      721 catcgtcgac cacaatcccg ccgcgttgtc catgaagcgt cgcctgctca gccgagagag
      781 ctaccaaatc attgaagcgg agaacggaac cgatgcgctc atgcttgccg ccgccgaact
      841 gccggatctc gtgctcctcg acgccaatct gccggacatc gacagcttcg atgcctgccg
      901 gcaacttaag actcaatctg agactaaatt catgaagata ctgcaaacct ccgccgctcg
      961 catgagcgcg ccggatcagc cgaggaacct ggatgtcggt cccgatgcct atctggttga
     1021 accgctggaa gaagaggagc tcattggaat cgtgcgggcc ttgctgaagc tcgcgcaaca
     1081 ggagcgggat aatctccagc tcgccgagaa gctctccctc gtcgaacgtc aattatcggc
     1141 cgcgaccgaa accgtccagt gcaccgaact caagcaaact gagcaaacgt tgcgagaaag
     1201 cgaagaacga ttcgcgaaag ccttccgcgc aagccctcat ccgatcggca tcactgaagt
     1261 ggcaaccggc cgttgcatag
//
LOCUS       DASTWE010000980         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_640277, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000980 DASTWE010000000
VERSION     DASTWE010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640277"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFT30_10210"
     CDS             complement(<1..295)
                     /locus_tag="VFT30_10210"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU5093051.1"
                     /translation="MRDKLLVQAKRIVIKIGSSLIASRAEGLRPQQIERLADEIATFR
                     TQGREILVVSSGAIVSGIRKLQLKDYPKSLPVKQAAAAVGQSRLMWAYEKAFER"
     gene            complement(277..1302)
                     /gene="obgE"
                     /locus_tag="VFT30_10215"
     CDS             complement(277..1302)
                     /gene="obgE"
                     /locus_tag="VFT30_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU5093052.1"
                     /translation="MFVDEVHIAVRAGRGGNGICSFRREMFVPRGGPDGGDGGDGGDI
                     VMTASHRLTTLLDLRYQNHYEAQDGRAGGGSNCTGRSGEDLTIAVPVGTIVYDDQTKE
                     TLADFIEDGQTAVIAQGGRGGKGNSNFATSVNRVPTKCTPGTPGEERTLRLELKLLAD
                     VGLVGFPNAGKSTLIAAISAARPKIADYPFTTLIPNLGVVRWGSDRSFVVADIPGLIE
                     GAHEGKGLGVQFLRHIERTAFLLHLIDVSEWAPDDPVVAFETLRRELSAYDLELTKRP
                     FAVVPTKIDVIGTSERLLQLQTYCRLNNYPCLPISAAANRGLDDLVTYLGKQVERLRK
                     TPCETSS"
     gene            complement(1423..>1610)
                     /locus_tag="VFT30_10220"
     CDS             complement(1423..>1610)
                     /locus_tag="VFT30_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5093053.1"
                     /translation="GGQTVTAGSIIVRQRGTKFFPGFNVDLGRDHTLFARTSGVVKFE
                     GGRGRRKVSVYPVPTKS"
BASE COUNT          362 a          445 c          443 g          360 t
ORIGIN      
        1 gacgttcaaa tgctttttca taggcccaca tgagccggct ctgccctacc gccgccgcgg
       61 cctgcttgac agggaggctt ttcgggtaat ccttcagctg caattttcgg ataccggaga
      121 cgatggcacc ggaagagacc accaagattt cccgaccctg tgttcggaac gtcgcgattt
      181 catcagccaa tcgctcgatc tgttgaggcc gcagaccttc cgcccgcgaa gcgatcaggc
      241 tacttccgat cttgatgacg atccgcttcg cttgtactaa gagcttgtct cgcatggcgt
      301 ctttcgtaaa cgttctacct gtttcccgag gtaggtgacc aagtcatcaa gccctctgtt
      361 agcggcggcg gaaatcggca agcagggata gttgttgagg cgacaatagg tctgcagctg
      421 gagcagccgt tcgctcgtgc cgatgacatc gatcttggtc gggacaacag cgaatgggcg
      481 tttggtgagc tccaggtcat aggcggaaag ttcccgccgt aaggtctcaa aagcgaccac
      541 tggatcgtca ggagcccatt ctgagacatc gatcaagtgg agcaagaacg cagtgcgttc
      601 gatatggcga agaaattgta caccaagacc tttcccctcg tgggcaccct cgatcaggcc
      661 gggaatatcg gccacaacaa aacttcgatc agatccccac cgaaccacgc cgagattagg
      721 gatcagagtg gtgaagggat agtcggcgat cttgggtcgg gctgctgata tggctgcaat
      781 gagcgtcgat tttccggcat tgggaaaacc gaccagcccg acatcggcga gcaatttcag
      841 ttcaagccgc aaggtgcgtt cttcacccgg agttccggga gtacatttcg tcggcactcg
      901 attgacggag gtggcaaagt tgctgttgcc tttcccgcct cgccctcctt gggcgatgac
      961 cgccgtttgg ccatcttcga tgaaatccgc aagtgtttct ttcgtctggt catcatagac
     1021 gatggtgccg acaggaacag cgatggtcag atcttctccg gaacggcccg tacaattgga
     1081 tcctccgccg gctcggccgt cttgagcttc ataatgattt tggtagcgga ggtcgagcaa
     1141 ggtcgttaat cgatgagatg cagtcatgac gatgtcgcca ccgtcgcctc catccccgcc
     1201 gtccgggcct ccgcgtggaa caaacatctc cctccggaaa ctgcagatgc cgttcccccc
     1261 gcgaccggct cgtaccgcga tgtgtacttc atcaacaaac ataatctgtc aacgttccca
     1321 ggcgaatcag gaattagggt gagaagtata cccgaccctg acccgaaggg gggagagaga
     1381 atttatccag tttggtcgta gcgaaaggag cagaaaagag aatcaggatt tggtcggaac
     1441 aggatataca ctgacttttc gtctgccgcg tccaccttca aacttaacca caccgctcgt
     1501 tctagcaaat agggtatggt ctcttccaag gtcgacattg aatccaggga aaaacttggt
     1561 gccgcgctga cggacaataa tgctgccggc tgttacggtc tgaccgccat
//
LOCUS       DASTWE010000981         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_705119, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000981 DASTWE010000000
VERSION     DASTWE010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705119"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFT30_10225"
     CDS             <1..291
                     /locus_tag="VFT30_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU5093054.1"
                     /translation="DKALGAIDSADDKVLHTLSTFVKQLPPGTMVKVRELTIDGATIL
                     MEGETTSFDAVDRLKQTFASSPQLKDVAVTETRVGAGPNQVVFRITVTVEKS"
     gene            288..836
                     /gene="gspM"
                     /locus_tag="VFT30_10230"
     CDS             288..836
                     /gene="gspM"
                     /locus_tag="VFT30_10230"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEU5093055.1"
                     /translation="MTQSFRERWRQMSQRERTLVLAGGIMLGLSLLFVLIVDPLLDTL
                     DRFERQEVRKQKELTQLAVLGQAYLAKQDRLAKSESRLPGTDSRFSLLTFMEEAATTA
                     HVRERITGMQPQVQSLTQGYEETAVDLRLESIQLPDLLALLVAIDQAPYDLHVRHLQI
                     RPKFDNPVNIDATVRVLSYAKS"
     gene            823..1767
                     /gene="gspK"
                     /locus_tag="VFT30_10235"
     CDS             823..1767
                     /gene="gspK"
                     /locus_tag="VFT30_10235"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEU5093056.1"
                     /translation="MRRADERGIALLLALLVLTILTALILEFDAEARREYRAAAAFRD
                     DYKASMLTRAAVQAARAVLQQDLLRERMTGQKYDGPTDIWAMPIKNYAIGDGFLTAQI
                     QDETGKFNLNSLASTSSGDIEQKKKVLRAKRLFELLRVSPNLVDALIDWMDQDEVPQP
                     AGAESLYYQSLKPPYQSANSPLPGLGDLRLVKGFTPEIIERISPYVTVFPQEGTAPMN
                     INTADPIVLQTLDPSVTQTVAMEIVQGRPYKTKVELDRVGSFQEIGRTLRSDYDIKSD
                     YFSAHLAVTVNETTKTAWAILKRDAGKGESTVEYLRVL"
     gene            complement(1806..2702)
                     /locus_tag="VFT30_10240"
     CDS             complement(1806..2702)
                     /locus_tag="VFT30_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5093057.1"
                     /translation="MNSLMTSDHLLVIDPCPDTQARIAEQLQGRGFSVVAVSDPTTAL
                     TTIDIAAPDILITDLFLPEATGLTLLKELKARHELCPVIVMAKDAPDHMIVQVLRIGA
                     ADYLHKPVTEEELAHALQRARNLLPADFADIPGLCRSEYRLTVNSEPTHIPRVISWLV
                     KTTGSTLPPIRRLHLRGALQELLFNAIEHGNLEIHYKEKQQALAEGQYNQLLAHKLAQ
                     ARLRDRQVIIHVLHEKHGNSLSYRITDEGKGFKWRSLFMQSQEVSESEESGRGIFLAR
                     SFFPCLTYNECGNEVTITVSLD"
     gene            complement(2699..3058)
                     /locus_tag="VFT30_10245"
     CDS             complement(2699..3058)
                     /locus_tag="VFT30_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU5093058.1"
                     /translation="MSDDTVYSMDEALARVDHDQETFQMMIELFMEHGPKDLADAEAA
                     LDAGNAAGVARASHKLKGAILQFCAPAALQACKELEESAKAGNLTHGEQLYASLEQEF
                     HRLLAALRQARDEGIAA"
     gene            complement(3084..>3398)
                     /locus_tag="VFT30_10250"
     CDS             complement(3084..>3398)
                     /locus_tag="VFT30_10250"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEU5093059.1"
                     /translation="RSGEVCWMARPSLPLGFDQSTTYLTTELPVRSGDRLYMLSDGLY
                     EVPASNGELWGQDRLKKTVHMFGHRPLTEVVSDIITEAVRWQGHEHFPDDVALMGIEI
                     AI"
BASE COUNT          787 a          990 c          912 g          709 t
ORIGIN      
        1 gacaaagcgc tcggtgccat cgattccgcg gacgacaagg tgttgcacac gctctcaacg
       61 ttcgtgaaac aactcccacc tggaacgatg gtcaaggtgc gcgagctgac catcgacgga
      121 gcgaccattc tcatggaggg cgagaccact tcctttgacg ccgtggaccg actcaagcaa
      181 accttcgcgt cgagccccca attgaaggat gtcgcggtga cggaaacccg agtgggcgca
      241 ggtcccaatc aagtcgtctt tcgaatcacc gtcaccgtgg agaaatcatg acgcagagct
      301 ttcgagaacg gtggcgccag atgtcgcagc gtgagcgcac cctcgtgttg gcaggcggga
      361 tcatgcttgg actcagcctc ctctttgtcc tgatcgtcga ccccctgttg gacacccttg
      421 atcgctttga gcgtcaggag gttcggaagc agaaagaatt gacccaactg gcggtgctcg
      481 gccaagccta tctcgccaaa caggaccgtt tggccaaaag cgaaagccgc ttgccgggaa
      541 ccgacagccg tttctccttg ctgacattca tggaggaagc ggccactacc gcccatgtgc
      601 gcgagcgtat cacgggcatg cagccgcaag tacaatcgct gacgcaaggc tacgaggaaa
      661 cggccgtcga tctccgattg gaaagtattc aattgcccga cttgttggca ttactcgtgg
      721 ccatcgatca agccccttac gatcttcatg ttcgccactt gcagatccgc ccgaaattcg
      781 ataacccggt caatatcgac gcgaccgttc gggtcttgag ctatgcgaag agctgacgaa
      841 cgaggcatcg cactgctctt ggcactttta gtgttgacga tcctcacagc ccttattctt
      901 gagttcgatg cggaagcacg ccgcgagtac cgagccgccg ccgcctttcg ggacgactat
      961 aaggcaagca tgctgacccg cgccgctgtg caggccgcgc gagccgtgct gcagcaggat
     1021 ctcttgcgtg aaaggatgac aggtcagaaa tacgacggac cgaccgatat ctgggccatg
     1081 ccgatcaaga attatgcaat cggggacgga tttctgaccg cgcagatcca ggatgagacg
     1141 gggaaattca acttgaacag cctcgcatcg acttccagcg gcgacattga acagaagaag
     1201 aaggtcctcc gagccaaacg attgttcgaa ctgctcaggg tcagcccaaa cttggtcgat
     1261 gccctcatcg attggatgga tcaagacgaa gtacctcagc cggccggcgc cgagagcctc
     1321 tactaccaat cgctgaagcc gccctatcaa tccgcgaata gcccgttacc ggggttggga
     1381 gatctcaggc tcgtcaaagg attcacgccg gaaatcatcg agcggatctc cccctacgtg
     1441 acggtcttcc cgcaagaggg aactgcgccg atgaacatca acaccgccga tcccatcgtc
     1501 cttcaaacgc tggatccgag cgtcacccaa accgttgcga tggagatcgt ccaaggacgc
     1561 ccctacaaga caaaagtgga actcgatcga gtcggaagct ttcaagagat cggtcgaaca
     1621 ctgagaagcg actacgatat caaatcggac tatttctccg cgcacctcgc cgtcacggtg
     1681 aatgaaacga ccaagaccgc atgggccatc ttgaagcgag atgccggtaa gggcgagagc
     1741 accgtcgaat acctgcgggt tctctagcag gatttgtccg aaccacgcca tcctctacac
     1801 gtcggttaat caagcgacac tgtgatcgtt acctcattac cgcattcatt atacgtcaaa
     1861 caggggaaga acgatcgagc caaaaaaatt cctcgcccgc tctcttcaga ctcagagact
     1921 tcctgggatt gcatgaacaa gcttcgccac ttgaagccct ttccttcatc ggtgatccgg
     1981 tacgatagac tgttgccatg cttttcgtga agcacatgaa tgatcacctg acgatccctc
     2041 agccgggctt gagcgagctt gtgagcgagt agctggttat attggccctc tgcgagagcc
     2101 tgttgttttt ctttgtagtg aatttcgaga ttgccatgtt caatggcatt gaagagcaat
     2161 tcttgtaacg ctccccgcag atgcaaccgt cggataggag gcagggtcga gcccgtcgtc
     2221 ttgacgagcc aggaaatcac ccttgggata tgggtcggct ctgaattgac ggtcagtcga
     2281 tattccgatc ggcagagtcc cgggatatcc gcaaagtccg cgggcaggag attccgagcg
     2341 cgctgcaacg catgcgccag ctcttcttca gtcacgggct tgtgcagata gtcggcggct
     2401 ccgatccgaa gcacctgtac gatcatatgg tccggtgcat ccttcgccat gacgatcacc
     2461 gggcagagtt catgcctggc cttcagctcc ttgagtaggg tcaggcctgt tgcctcagga
     2521 agaaacaggt cggtgatcaa aatgtccggt gccgcgatgt cgatcgtggt caaggcggtg
     2581 gtcgggtcgg agacagcaac gactgaaaac cctctgccct gcaactgttc ggcaatgcgt
     2641 gcctgagtgt ccggacaggg atcgatcacc aggagatgat cggatgtcat gagactattc
     2701 atgccgcaat tccttcgtcg cgcgcctgac ggagtgcggc aagaagacga tggaattcct
     2761 gctccaacga ggcatagagc tgctcaccat gcgtcaagtt tcctgctttc gccgactctt
     2821 ccagctcttt gcaggcttga agggcggctg gggcacaaaa ctgaagaatc gcccccttca
     2881 acttgtgact ggctcgcgcc acaccagcgg cattcccggc atccaaggcc gcctcagcgt
     2941 ccgccaagtc cttcggacca tgttccatga acaattcgat catcatctgg aacgtctctt
     3001 ggtcatgatc aacacgggca agggcctcat ccatactata gacagtatca tcgctcatga
     3061 gttcacctct caaaccaccg cggtcaaatg gctatttcga tacccatcag cgccacatca
     3121 tcaggaaagt gttcgtgccc ctgccatcga actgcctccg taatgatgtc ggatacgact
     3181 tccgtgaggg gacgatggcc gaacatatga accgtctttt tcagccgatc ttgtccccag
     3241 agctcgccgt ttgaagccgg cacttcatag agaccatcgc tcagcatata caaccgatca
     3301 cctgacctga ctggaagctc cgtcgtgagg taggtcgtcg actgatcgaa tcccagcggc
     3361 aaactgggtc tggccatcca acagacctca ccggagcg
//
LOCUS       DASTWE010000982         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_801872, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000982 DASTWE010000000
VERSION     DASTWE010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801872"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..564
                     /locus_tag="VFT30_10255"
     CDS             151..564
                     /locus_tag="VFT30_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093060.1"
                     /translation="MNGGRRISSIRLLLFYEAVVFLMAAFVHAGMWIDGYRHREARIA
                     ESVLAGALYVGLGLSWLRPLWARKAGLVAQGFALFGTLIGMFTIIVGVGPRTNPDILY
                     HLCMVGLLSWGLLYTIRAPTDLSEKAERPCPQRAA"
     gene            660..1043
                     /locus_tag="VFT30_10260"
     CDS             660..1043
                     /locus_tag="VFT30_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5093061.1"
                     /translation="MKNKVTWFEIPASDFQKAKKFYETVFDWKIDLWGDEGAMATTTA
                     VDKDQNPIEPGGINGGFYKRKSKDEYPSFVVQTESIDKTLKAIETGGGKVVTPRHSIG
                     EWGFMADFADPEGNVVALWEKPKTK"
     gene            1091..1690
                     /locus_tag="VFT30_10265"
     CDS             1091..1690
                     /locus_tag="VFT30_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEU5093062.1"
                     /translation="MYRVLSILTCLTFLFIGTPVFAKESKEKKAKDPQAMMETYQKLA
                     TPGEPHKQLASLAGTWATHTKEWMEPGTPPTESTGTCEFKVLLDGRFVQQECSGQMMG
                     QSFTGIGTHGYDNFTKKYITTWIDSMGTSIFSMEGKAGANDKTITLKGSHADPFEGVM
                     QHRAVWKFPDADTQIFEMYGSHGKGKEMKMMEITYTRKS"
     gene            1734..2036
                     /locus_tag="VFT30_10270"
                     /pseudo
     CDS             1734..2036
                     /locus_tag="VFT30_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT          583 a          544 c          629 g          454 t
ORIGIN      
        1 aggaatgtag ccgctttgcg caaaggccgc tgacgttcgg ctcacggcag atccacgtta
       61 agcagcagat aaaaaaccca tctcgtctag tgatcgagga gtgatcaagg aatagagcga
      121 gaacaatgga tcggtgaata gaaggcatcc atgaacggtg ggaggagaat ctcgtctatt
      181 cgattgctgc tattctatga agccgtcgtg ttcctcatgg ccgcatttgt ccatgcggga
      241 atgtggatcg acgggtatcg gcatcgagag gcgcgaatcg cggaaagcgt gctggccggt
      301 gcgctctatg tcggacttgg cctcagttgg ttgcgtccac tctgggccag aaaggcgggt
      361 ctcgtggctc agggatttgc actgttcgga acgctcattg ggatgttcac gatcatcgta
      421 ggggtcgggc cgcggacaaa tccggacatt ctctatcacc tctgcatggt aggccttttg
      481 tcatgggggc ttctctatac catccgcgca cccaccgacc tgagcgaaaa agcagaacgg
      541 ccatgtcccc agcgcgccgc ataagatgct tcatgtcgat ttttgaatta cggcacgact
      601 actgatgaat ggcaagagag acagatcgca caaactattc tcaaacaacg gaggatcaga
      661 tgaagaacaa agtcacatgg tttgaaattc cggcctcgga ctttcagaag gcaaaaaaat
      721 tctatgaaac ggtattcgac tggaagatag acctctgggg cgatgaagga gcaatggcca
      781 ccaccacagc ggttgataaa gatcagaatc cgattgagcc gggcggcatc aacggagggt
      841 tctataagcg caaatccaaa gacgagtacc cgtcattcgt cgtgcaaacg gaatcgatcg
      901 acaagacgct gaaggccatc gagacggggg gtggaaaggt cgtgacgccc agacattcca
      961 tcggggaatg gggcttcatg gcggatttcg cggatcccga aggtaacgtc gtcgctctct
     1021 gggaaaagcc caaaacaaaa tgaggaacga tctagcgtac taacctttat tcgtttttca
     1081 ggaggtccgt atgtatcgcg tattatctat actcacatgt ctgacgtttc tcttcatcgg
     1141 cactccggtt ttcgccaagg aaagcaagga aaagaaggcc aaagatccac aggcgatgat
     1201 ggaaacgtac caaaaactgg ccacgcccgg ggagccccat aaacaactgg cgagccttgc
     1261 aggcacatgg gcgacgcaca cgaaagaatg gatggaaccc ggcacgccac cgacagaatc
     1321 cacgggcacg tgcgaattta aagtgctgct cgacggccgt ttcgtgcaac aggaatgctc
     1381 aggccagatg atgggccagt cctttaccgg catcggcacg catgggtatg acaatttcac
     1441 caagaaatat ataacgacgt ggatcgattc aatgggaacc agcatttttt ccatggaagg
     1501 gaaggccggc gccaacgaca agaccattac gctcaaaggg agtcacgccg atccgttcga
     1561 aggcgtcatg caacatcggg cagtctggaa gtttccagac gccgatacgc aaatttttga
     1621 aatgtacggc tctcacggca agggcaaaga gatgaagatg atggagatca cgtataccag
     1681 aaaatcttag ttcaacgctt gaagggcgcc gggtgagatg accgggtact cctggcgccg
     1741 ccccgcactt tcatcgaatc atgtcggaat catttttcat cttgtgcggc atggtgcgct
     1801 cgttcaatgg agaacgttgg atcaatgcca cggcgggaga cctcctctat gtgcctgagg
     1861 gtggacgaca tggtttccgt aacgaagccg gcgaaccgac ctcgatgttg atcctctttg
     1921 caccggcgcc gcgagagggg catttcgaag ccctggctca gaaagcggcc ggccgccggt
     1981 tcagcgacga ggagtggctg gatctctgcc gccaccacaa caactacttt gtctgagcgt
     2041 ggtgctgatc gggggtaagc taaagcagca gcaaaagtta tctttttatc gctcgataga
     2101 aggacgacaa gtgtgacgaa gcataagtcg gaacatgttg atatgagagg ggctgtatga
     2161 agcagacgga agaaaaagcg cagccaaaga ctcatgcacc tcgttcatcc
//
LOCUS       DASTWE010000983         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_866544, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000983 DASTWE010000000
VERSION     DASTWE010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866544"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..2005
                     /locus_tag="VFT30_10275"
     CDS             152..2005
                     /locus_tag="VFT30_10275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEU5093063.1"
                     /translation="MDNLTRQLNERFGFATFRPGQEDVIRAVLAGRDAMAIMPTGQGK
                     SLCYQLPATLLPGLTLVISPLIALMQDQVTAMRQRKIAAAAFHSGLSGLEKSRLMQDL
                     QQRQVQLLYLAPERMQHEGFLRLLRSLWVSLLVVDEAHCISQWGHDFRPDYLKIGRLR
                     GELTNPPCLALTATATARVQTDLCQRLSLRDPFRLVTGFRRANLALSVHHCQTLREKL
                     GMVERLVCGSGKGTILIYCATRRTVEEVAGWLGQSHQAVGYYHAGLSDEQRRLVHDDF
                     RRGMLRILVATNAFGMGIDKPDVRLVLHFDIPGSLEAYYQEAGRAGRDGQPAACVLLF
                     HERDVATQEYFIAQASKDSEGAERAERMTTLLQELLGYVSVSTCRQVAILDYFSDQVE
                     LALGSCGLCDRCGAKPSRMISHETAATAKAIVETVSWCGGRFGMGRIVDILRGSRSRA
                     LLAYGVEDCSTYGIGRAQTKVSVTNLVKTLIGSGYLEVQGTEYPTLDVTPKGREILQG
                     LRTIALEQAEEYSPDGSLKKPRPSPAAQMAIPKASSPDRQLVERLRQLRSELAKEEGV
                     APFLIFHERTLKAIADYKPETPTALLEIPGIGEMKAERYGRRVLAVVNEDQ"
BASE COUNT          418 a          548 c          627 g          457 t
ORIGIN      
        1 accgttttcg tataatcgat cgatgtgtaa gcgcgtgtga atgaagacaa cgagatagac
       61 acagtagtat tgggtggatt gatggtgacc ctatctcaac cagctcgcct tgcccgcacc
      121 gacatgcttt ctctctgatt cacgtatctc catggataat ctgactcgac agctcaacga
      181 acgattcggg tttgcgacgt tccggccggg ccaggaggac gtcattcgcg ccgtattggc
      241 ggggcgcgat gcgatggcga tcatgccgac ggggcagggg aagtcgctct gctatcaatt
      301 gccggcgacc cttctccctg ggttgacact ggtgatttct ccgctcatcg cattgatgca
      361 agatcaggtg acggccatga gacagcggaa gattgcagcg gcggcgtttc actccggtct
      421 cagcggtttg gaaaagagcc gcctgatgca ggatctccag cagcggcaag ttcagctgct
      481 ctatctggcg ccggaacgga tgcagcatga agggtttctc cgactgttgc gttccttatg
      541 ggtttcgcta ttggtggtcg atgaagcgca ctgcatttcc cagtggggtc atgacttcag
      601 gcccgactat ctcaagatcg gtcgcctgcg tggggaactc acgaatccgc cctgcttggc
      661 cttaacggcc acggccaccg ctcgtgtgca gacagatctg tgccagcgat tgtcccttcg
      721 tgatccgttc cgattagtca cgggttttcg gcgggcgaat ctggccttgt ccgtacatca
      781 ttgtcagacc ctccgagaga aactggggat ggtggagcgc ttggtttgcg ggtcagggaa
      841 gggcacgatc cttatttatt gcgcgacccg ccggacggtg gaagaggttg cgggctggtt
      901 gggacaatcc caccaagcgg tcggctacta ccatgccggt ctctcggatg agcaacggcg
      961 actcgtgcat gatgatttcc gacggggaat gttgagaatc ctggtcgcga cgaatgcctt
     1021 cggcatgggt atcgataagc ccgatgttcg actggtcctc catttcgaca ttccgggcag
     1081 tctggaggcc tattatcagg aggccggtcg tgccggccgc gacggacagc ctgccgcctg
     1141 tgtgttgctg ttccatgaac gcgatgtggc cacgcaggaa tatttcattg cccaggcgtc
     1201 aaaggattct gaaggcgccg agcgtgcgga acgcatgacg acactgctac aagaactcct
     1261 gggctacgtg tccgtgtcaa cctgccggca ggtcgcgatt cttgactact ttagcgatca
     1321 agttgagctg gctctcggat cttgcggcct atgtgatcgc tgtggtgcga agccgagccg
     1381 gatgatctct catgagaccg ccgcgaccgc gaaggcaatc gtggagaccg tgtcctggtg
     1441 cgggggacga ttcggtatgg ggcgaatcgt cgacattctt cgtggaagcc gatcaagagc
     1501 gctgctggcc tacggcgtag aagactgttc gacctacggc atcggtcgcg cccagacgaa
     1561 ggtatcggtg acgaatctcg tcaagactct catcggctcc ggatatctcg aggtccaagg
     1621 tacggagtat cccacgctgg atgtgacgcc caaaggacgg gagattctgc aagggttacg
     1681 caccatagcc ttggaacagg cggaggaata ttcgccagat ggatcgctga agaaaccgcg
     1741 tccctcgcct gccgcccaaa tggcaatccc gaaggcttcg tcgccggatc gacagcttgt
     1801 tgaacgactc aggcagcttc gttcagaact tgccaaggaa gagggcgtcg cgccattcct
     1861 catttttcac gaacgaacgt tgaaagccat tgccgattac aaaccggaga cgccgacagc
     1921 cttgctagag attccgggta tcggggagat gaaagcagaa cggtatggcc gtcgggtatt
     1981 ggctgtggtc aatgaagatc aatagcccag tgactttcat cgggttgtga aagcaccctg
     2041 catctgtggc
//
LOCUS       DASTWE010000984         4275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1577627, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000984 DASTWE010000000
VERSION     DASTWE010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577627"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFT30_10280"
     CDS             complement(<1..192)
                     /locus_tag="VFT30_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093064.1"
                     /translation="MIDHGELAEGTIAIGFGCIRPVSSSRKASLVWAGDDRVQIGIHN
                     RVAVQHFDDRLTGTRVTYRV"
     gene            181..>4275
                     /locus_tag="VFT30_10285"
     CDS             181..>4275
                     /locus_tag="VFT30_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093065.1"
                     /translation="MIYHDEIQDIQAFPQFRDPILKHTLHSVEDKFAINYGVAGAGAE
                     ILANRLGVGPMFNCTECKYEEFFLSSWTIGDPAQIVDVPANTTDKLGNLITGRKATKV
                     LYPDDPSNVHHSYIGDPVKMRVVHAGPKEHHIHHLHAHQWLQTPDDDNSTYLDSQALG
                     PGFAFTTEIVHGGTGNRNQTVGDSIFHCHFYPHFAEGMWELWRAHDVFETGTALDANG
                     IPVAGSRALPDGEIIAGTPTPALVPLPTLAMAPMPTASVPGYPFFNPAMAGHRAPKPP
                     LDTIDDGGLPRHIITGGTFTHVETRLDFSKELVTATATKIAETGTAAEIAAMVYHEQR
                     LHASFKPDGTPANFVLNGLPRKPGAPFADPCIDDNGNAKGNPRTYKAAAFQLDIKLNK
                     AGWHFPQSRILSLWADVAPTQAGTKPPEPFFFRANTNDCITFYHTNLVPGVYEQDDFQ
                     VRTPTDIIGQHIHLVKFDVTSSDGSGNGFNYEDGTFSPDEVRERIKAIRKQNGCIGIE
                     SGDTRDGTFTCPKPKAHPYFGTLGAQTTVQRWFADDTLNNNGFDRTLRTVFTHDHFGP
                     STHQQAGLYAGLVIEPQGSVWKNPETGVTMGTRTVDGGPTGWQANIIETTAANSHREF
                     LFEFADFQLAYQAGAGVGANGKPIADPQRVINPPIINEVGLPFLVENGMVCPGGVPAP
                     CPEAVSAADQGTMSVNYRNEPVPLRIRDPYTNTQAAGAPGDLSKVYKSNITRADPQFN
                     VQPTFYPALTKGVEPGDPFTPLLRVYENDKVQIRILVGAHEETHNLTINGHKWLHQPG
                     TPQDPLAVNNSGFRNSQIAGISEHFEFLTGKESILGGRPFIDYLYQNSASVDGQWNGV
                     WGIMRVYNGRMGLKTDLLALPNNLEGAAALTTNDTEFPVDSTFPNGATDFKDATDASV
                     ETYSTPTTDTRFMSPTMLGTATSAVSLDGMTTQSAIIGPIAIEEEIVMAMPTDFPTGK
                     SSGASICPGSAPKRKINVTAVNAWALPAGKLIYNSRAGNGGALNDPTAIMYFRSEDID
                     SYGRVKAGVPIEPLVVRARAGECIQFNLENRLPKTLPDLDGFNTMSNLLFHFNTNQVK
                     PSNQVGLHPQLLAYNVANSDGKNVGFNPNQTVGPGGVVRYSWYAGDVVINGGRRIGTP
                     IEFGATNLISSDPIKHSNKGAIGSLIIEPQGSTWIEDSTSRAQATVTKSDGTSFREFV
                     IQFQTDINLRFADGSAVPNLGGPGGAEDPEDSAQKAINYRTEPLWKRMNYAPQTPFEN
                     TRNFDFTNVLTNAQVGGDPQTPVFTAQAGQAVRFRILNANGHMRNNVFNLHGHFWQDE
                     PFTNNSKSIGDNPLSEFKGTVYGIGPSSHYEVIPVNGAGGARRVT"
BASE COUNT         1060 a         1237 c         1119 g          859 t
ORIGIN      
        1 tacccggtag gtcaccctcg tgccggtcag ccgatcatca aaatgctgaa cggcgacacg
       61 attgtgaata ccgatttgaa cgcgatcatc accggcccaa acaagggacg ctttccggct
      121 ggaacttacc ggccgaatgc aaccgaaccc gatcgcgatc gtcccttccg cgagttcacc
      181 gtgatctatc acgatgaaat tcaggatatc caggccttcc cgcagtttcg cgatccgatt
      241 ctgaagcaca cgctccacag tgttgaagac aagtttgcga tcaactacgg agtggcaggc
      301 gcgggcgcag agattctcgc gaatcgactc ggcgtagggc caatgttcaa ttgcactgag
      361 tgcaagtatg aagagttctt cctttcttca tggaccattg gcgacccggc gcagatcgtg
      421 gacgttcccg cgaacaccac cgacaagctg ggcaatctga tcacaggtcg caaggctaca
      481 aaagtcttgt atcccgacga tccttcgaat gtgcatcaca gctatatcgg cgacccggtg
      541 aagatgagag tcgtgcacgc gggaccgaaa gagcatcaca ttcaccactt gcatgcgcac
      601 cagtggctcc aaacacctga cgacgacaat tcgacgtatc tcgatagtca ggcacttgga
      661 ccaggcttcg cgtttacgac tgaaatcgtt cacggcggca ctggaaaccg aaaccagact
      721 gtcggcgatt cgattttcca ctgtcacttc tacccgcact tcgctgaagg gatgtgggaa
      781 ctgtggcgtg cacacgatgt gtttgagacg ggaacggccc tcgatgccaa tggcattccg
      841 gtagccggtt cacgcgcgtt gcctgacggc gaaatcatcg cgggcactcc aacacccgct
      901 ctggtgccgc tgccaacact cgcaatggcg ccgatgccga cagcgagtgt tccgggttat
      961 ccgttcttca atccggcaat ggcaggccat cgcgctccca agccgccact cgacaccatc
     1021 gacgatggcg gattgccgag acacatcatc acgggcggaa ctttcacaca cgttgaaacc
     1081 cgtctcgatt tcagtaaaga actagttacg gcgaccgcga caaagatcgc cgaaacggga
     1141 actgctgctg agatagcagc gatggtctat cacgagcaac gactgcacgc gagcttcaag
     1201 cctgacggca caccagccaa cttcgtgttg aatggtctgc cgcgcaagcc cggtgctccg
     1261 ttcgcagatc catgcatcga cgacaacgga aatgcgaaag ggaatccccg cacttacaaa
     1321 gcagccgcat ttcaactcga catcaaactt aacaaagccg gctggcactt cccgcagtcg
     1381 cgcatccttt cactctgggc ggacgtggct ccaacccagg caggcacaaa accgccggaa
     1441 ccatttttct tccgtgcgaa taccaacgat tgcatcacgt tctatcacac caacctcgtt
     1501 cctggcgttt atgagcagga cgactttcag gtacgtacgc cgaccgacat catcggtcag
     1561 catattcacc tcgtgaagtt tgacgtcacc tcgtcagacg gctctggtaa cggcttcaac
     1621 tatgaagacg gaaccttcag ccccgacgaa gtgcgagaac gaatcaaggc cattcgcaaa
     1681 cagaatggct gcatcggtat tgaatcaggt gacacacgtg acggcacctt cacctgtccg
     1741 aaaccgaaag cacatccata cttcggcact ctcggcgcac aaactaccgt gcaacgctgg
     1801 tttgccgacg acacactcaa caataacggc ttcgaccgta cgctcaggac cgtgttcact
     1861 cacgatcact ttggaccatc gactcaccag caggcaggtc tctatgccgg cctggtgatc
     1921 gagccgcaag gttcggtctg gaagaatccg gaaacgggcg tcacgatggg tacccgtaca
     1981 gtggatggtg gcccgacggg ctggcaggca aacatcatcg agacaactgc ggcgaacagt
     2041 caccgcgaat tcctgtttga gtttgctgat ttccaacttg cttatcaggc aggcgccggc
     2101 gtcggagcca atggcaaacc gattgccgat ccgcaacgag tcatcaaccc gccgatcatt
     2161 aatgaagtgg gactaccttt tctcgtagag aacggtatgg tttgtcccgg tggtgttccc
     2221 gctccttgtc cggaagcagt ttccgcggca gaccagggca cgatgagtgt taactaccgg
     2281 aatgaacccg tgcctctgcg cattcgcgat ccgtatacca acacacaggc ggccggcgct
     2341 cctggtgatt tgtcgaaggt ttacaaatcg aacatcaccc gcgcagatcc gcagttcaac
     2401 gttcaaccaa ctttctatcc ggctttgact aagggagtag aacccggaga tccattcaca
     2461 ccgcttctga gagtttatga gaatgacaaa gttcagattc gtattctcgt gggagcacat
     2521 gaagaaacac ataacctgac aatcaacggt cataagtggc tgcaccaacc gggcacgccg
     2581 caggatccat tggcggtcaa caactccggt ttccgaaact cgcaaattgc cggtatctca
     2641 gagcacttcg agtttctcac cggaaaagaa tcgatcctgg gcggccggcc ctttatcgat
     2701 tatctgtacc agaacagcgc gtcggtcgac ggacagtgga acggcgtgtg gggaatcatg
     2761 cgagtctaca acggccgcat ggggttaaag acagacctgc tcgccttacc aaacaatctg
     2821 gaaggcgcgg cagcgttgac gaccaacgat acagaattcc cggtggattc aactttcccg
     2881 aacggtgcaa ccgacttcaa ggatgcgacg gatgcctctg ttgagacgta ttccacgcct
     2941 acgacagata caaggtttat gtcaccaaca atgctgggca cggccacctc ggctgtctca
     3001 ttggacggta tgacgactca aagcgccatc atcggtccaa tcgccattga ggaggagatt
     3061 gtgatggcga tgcccaccga tttcccaacc ggtaagagct ccggcgccag tatttgtccg
     3121 ggctcggctc cgaagcggaa gatcaacgtg acagcggtca atgcgtgggc tcttccagcc
     3181 ggcaagctga tttacaacag ccgcgccgga aacggcggag cactcaacga tccgacggcg
     3241 atcatgtact tccgcagtga ggacatcgac tcctatggca gagtgaaagc gggcgttcct
     3301 attgaaccgc tcgtcgttcg cgccagggca ggtgaatgta tccagttcaa tctcgagaac
     3361 aggttgccaa aaacgctacc cgatctcgac ggtttcaaca cgatgtcaaa cctgctcttc
     3421 cacttcaata cgaatcaggt gaaaccatcg aatcaggttg gtttgcaccc gcaactgctc
     3481 gcctacaacg tagcgaacag tgatggtaag aacgttggct tcaacccgaa tcagacggtt
     3541 ggaccaggcg gcgtagtcag gtatagctgg tacgccggag acgttgttat caacggtggc
     3601 aggcgtatcg gaacgccgat tgaattcggc gctacgaacc tgatctcatc cgacccgatc
     3661 aagcacagca acaagggtgc aatcggttca cttatcatcg aacctcaggg atccacatgg
     3721 atcgaagatt cgacttcacg tgcgcaagcc acagtgacca aatctgatgg cacgtcgttc
     3781 cgtgaattcg taatccagtt ccagacggac atcaacttga ggtttgcaga tggcagcgca
     3841 gtgccgaatc tcggtggtcc aggtggagcg gaagatccgg aagactcagc gcagaaagcg
     3901 attaactatc gcactgagcc actctggaaa cgcatgaact acgcgcccca aacaccattc
     3961 gagaacactc ggaacttcga cttcacgaac gtgctgacca atgcgcaggt gggcggcgat
     4021 ccacaaacgc cggtcttcac cgcacaggct ggtcaggcag tgaggttcag aattctgaat
     4081 gccaacggtc acatgcgcaa caacgtgttc aacctgcacg ggcacttctg gcaggatgag
     4141 ccattcacca acaactcgaa gtcgattggc gacaatccgc tttcagagtt caagggaacg
     4201 gtttatggaa tcggaccttc gtcacattac gaagtgattc cggtgaacgg agccggtggc
     4261 gcacgtcgcg taaca
//
LOCUS       DASTWE010000985         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_672867, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000985 DASTWE010000000
VERSION     DASTWE010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672867"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFT30_10290"
     CDS             <1..181
                     /locus_tag="VFT30_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093066.1"
                     /translation="GQFRVDLDRQSAGRSFQHGPSQEQGHTWDQGSQGMKWGESPDRP
                     DEQRTSLHGLLNVMA"
     gene            203..856
                     /locus_tag="VFT30_10295"
     CDS             203..856
                     /locus_tag="VFT30_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEU5093067.1"
                     /translation="MIDVSELNSTGTKATSEKTGPRQLGQDDFLKLLITQLKNQDPLK
                     PTDNAEFVSQLAQFSQLEQTAKQAQLLQKSLDAQTASLQFTLLPMVGRRVSSDRPLTQ
                     LENGQASLTYALEKNAARVQITILDQSRQVVRTLDSTGLQAGLNQAQWDGKDSKGAVM
                     PPGVYEYALSAVDHQGASVVAKGRAQLTVTGVRMEEGQPKLAVGTITVDPSEIVEVQ"
     gene            899..2635
                     /locus_tag="VFT30_10300"
     CDS             899..2635
                     /locus_tag="VFT30_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEU5093068.1"
                     /translation="MGILSSLFAGVSGLNANGTALSVIGNNIANLSTVGFKGSKATFA
                     DLISSSISGGSGAIQTGIGVALTSVQGNFSQGSLATSSNVLDLAIDGNGFFIVEDAQG
                     GTFYSRAGLFRLDKNNNVVDPTGFKLQGFLADTTGTITGTIGDIALPSTTASPRATAT
                     AFVAANLNSATTPSGVRGNVVASAASTTTTAAGNNSFTINLNGDGAQTITVANGLTGS
                     ALATAIQNAVRALVPNDSFKGAAYAGFTASVNASNTFTFTSGMTGTTNNSTTGTGTVV
                     VTANGGDTLAANLNMLAPTSTTGTDFLLSDPPATSNFSTSMTVFDSLGNSHLLTTYFT
                     KIGENSWNYNTVAAASDVVTANYHSSNIDSSLGIVRVGSGTLTFGTDGTLDRESAVIR
                     YDTGTAAGTSGTVPGELQIDFTGATQDQLMVMNFGSSVTTDGGSGLDGTTQFGSTSAL
                     VQQTQDGFAAGSLQAFSVDANGIINGRFSNGQLRALAQVVLARFPDPIGLARTGKNTF
                     AQSGNSGQPVTGTPDSAGLGRVLSNSLELSNVDLGESFIDMIAAQRGFQANSRVITTS
                     DEILQELVNLKR"
BASE COUNT          615 a          803 c          841 g          591 t
ORIGIN      
        1 gggacaattt cgcgtggacc tcgatcgtca gagtgctgga cgttccttcc agcatggccc
       61 ttctcaggaa caaggtcaca cctgggatca aggttcgcag gggatgaaat ggggagagag
      121 tcctgaccga ccagatgaac aacgcacgtc gctccatggc ctattgaatg tcatggcgta
      181 gatcaaaagg gagatgccgg aaatgatcga tgtatcggaa ctcaattcga caggaaccaa
      241 agcgacatcg gaaaaaacag gacctcgaca gttggggcag gacgattttc tcaaattgct
      301 catcacgcaa ttaaagaatc aggatccgtt gaagcccacc gacaacgccg aatttgtctc
      361 gcagctcgct caattcagtc agttggaaca gaccgcgaag caggcgcagc tgcttcagaa
      421 gagcctcgat gcgcagaccg cctcgctgca gtttacgctg ttgcccatgg tcggccgccg
      481 cgtcagcagc gatcgtcccc tgacccaatt ggagaatggt caggcctcgc tgacgtatgc
      541 actcgaaaag aacgcggctc gcgtgcagat tacaattctc gaccagagtc gccaggtcgt
      601 gcggacgttg gactccaccg gcctccaagc aggcctcaat caagcacaat gggacggaaa
      661 agacagtaag ggcgccgtca tgccccccgg cgtgtatgag tatgcactct cggccgtcga
      721 tcatcagggt gcctcggttg tcgcgaaagg ccgcgcgcaa ctcaccgtta cgggcgtgcg
      781 tatggaggag gggcagccga agttggctgt cggaacgata accgtggatc cgtcggagat
      841 cgtggaagtg cagtaacagg gacttatccg aacaggctca agaaggaggc atgcacccat
      901 gggaatactg tcgtcattgt ttgccggagt cagtgggctc aacgccaatg gtacggcact
      961 ctccgtcatc ggcaataaca tcgccaacct cagcacagtc ggcttcaagg ggagcaaggc
     1021 gacgtttgcg gacttgatca gttcgtcgat ttcaggcgga tccggtgcga ttcagacagg
     1081 catcggtgtg gcgctcacgt ccgtgcaggg gaacttctct caaggatcgc tggcgacctc
     1141 gtccaatgtg ttggaccttg ccatcgacgg caacggattt ttcatcgttg aagacgcgca
     1201 gggcggcacc ttctattccc gtgccggcct gttccggctc gataagaaca acaatgtggt
     1261 ggatcctacg ggattcaaat tgcaaggctt ccttgccgac accaccggca ccatcaccgg
     1321 tacgatcggc gatatcgcgc tgccttctac cactgcatcg ccgcgggcca cggccacagc
     1381 gttcgtcgcc gctaatttaa actccgcgac cacgccgtct ggtgttcggg gaaatgtcgt
     1441 ggcttctgcg gcctcgacga ccacaactgc agccggtaac aattctttta ccatcaattt
     1501 gaacggtgat ggggctcaga caatcaccgt ggcgaacggg ttgacaggtt cggcacttgc
     1561 cacggcgatt caaaacgccg tgcgcgcgct agttcccaac gattccttta agggcgccgc
     1621 ctatgcggga ttcaccgcat cggtcaatgc ctccaatacc ttcacgttca cgtcgggtat
     1681 gaccgggacg acgaacaatt ccacgaccgg taccgggacg gtggtggtca cggcgaacgg
     1741 cggcgatacg ctggcggcca acctcaatat gctggcgccg acgtcgacga ccggcacgga
     1801 tttcttgcta tcggatccgc ctgccacctc gaatttttcc acgtcgatga cggtgtttga
     1861 ttcactgggc aacagccatc tcctgacgac atatttcacg aagatcggtg agaacagttg
     1921 gaactacaac acggtggcgg cagcgagcga cgtcgtgacg gcgaactatc attccagcaa
     1981 catcgacagc agtttgggca tcgttcgagt cgggtccggc accttgacgt tcgggacaga
     2041 cgggacattg gatcgagaga gcgcagtcat ccggtacgat accgggacag cagccggtac
     2101 gtccggtacg gtgccgggag aattacagat tgattttacc ggtgcgacgc aagaccagtt
     2161 gatggtcatg aatttcggtt ccagcgtgac gaccgacggc gggagcgggc ttgatgggac
     2221 gacgcaattc ggatcgacct cggctctcgt gcagcaaaca caggatgggt ttgccgccgg
     2281 ctcgcttcaa gccttttcag tggatgcgaa cggcatcatc aacggacgct tctcgaacgg
     2341 tcaattgcgc gcgttggcgc aggtggtgct cgcgcgcttc ccggatccca tcgggttggc
     2401 gaggaccggt aaaaacacct tcgctcagtc cgggaactcc ggtcagccgg tcacgggaac
     2461 gccggacagt gccgggctcg ggcgagtgtt gtcgaattcg ttggaactgt cgaatgtgga
     2521 tctcggtgaa agttttatcg atatgatcgc tgcgcaacgc ggttttcagg cgaactcgcg
     2581 cgtgatcacc acgtccgatg aaatactaca ggaattggtc aatctgaagc ggtagatcct
     2641 tctctcgcgg tggggctgtg agcgatagcg atctcccagc cccacttccc ttcagtcgtc
     2701 ctcccatccg gcggtttggc gctgctccgg gagccctgtc accccgcatc ccctgtcagc
     2761 ttattgacgg tgtcaagagg tcgcttgaca gggtgttcat acttcgtgct gtccgcggtg
     2821 cgtttagtgt tggattatgc gccagatata
//
LOCUS       DASTWE010000986         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_123381, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000986 DASTWE010000000
VERSION     DASTWE010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123381"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFT30_10305"
     CDS             complement(<1..544)
                     /locus_tag="VFT30_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093069.1"
                     /translation="MVNLVSATLQLYRHVLYATGRSFARSWVTLVALIAFAVLLFGTS
                     RIASPFGIAGGFILGIVNALLVGATLRLIELSLSAARSIQLTDVTESFGHYFWDVIGV
                     GFVLWLPTMLLDMGMQANPYGQFLLSAFLLLVFILLNPAPEVIYQVRHDSTLNVFKTS
                     YEFVLEHWVEWFLPFAILILP"
     gene            679..>1974
                     /locus_tag="VFT30_10310"
     CDS             679..>1974
                     /locus_tag="VFT30_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5093070.1"
                     /translation="MDANRMTIKLQEALQSASAHAQRRSHQGIDVEHVLLALLDQEGG
                     TTLALLEGAGLALPAVRQVVEQALAKLPQVQGSGAAPGQMHITPRLTQVLNRAEDEQK
                     SLKDDFLSVEHVLLAMVQEGGVLKKLGLTRDRLLAGLQQVRGNQRVTSQDPESTYQSL
                     VKYGRDLTQLAGQGKLDPVIGRDDEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AIRIVKGDVPESLKQKKLFALDMGSLVAGAKFRGEFEERLKAVLKEIQSSQGQILLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGELHLIGATTLDEYRKHIEKDAALERRFQT
                     VLVDQPSVENTISILRGLKERYEVHHGVRIKDSALVAAAKLSHRYISDRFLPDKAIDL
                     VDEAAARLRTEIDSLPAELDEVSRKVLQLEIEREALKKEK"
BASE COUNT          510 a          531 c          556 g          377 t
ORIGIN      
        1 ccggcaagat gagaatcgcg aaggggagga accactcgac ccaatgctcc aacacaaact
       61 cataagatgt cttgaacacg ttgagcgttg aatcgtgtcg gacttgatag atcacctccg
      121 gcgccggatt caagagaatg aataccagaa gcagaaatgc cgacaaaaga aactgcccat
      181 atggatttgc ttgcattccc atatcgagca gcatcgtcgg cagccacagc acgaatccca
      241 cgccgatgac gtcccaaaag taatgcccga agctttcagt cacatccgtg agctgaatgg
      301 atctcgcagc gctgagtgac aactcgataa gccggagggt cgcgccgacc aacaaggcat
      361 tcacgatgcc caggatgaac ccgcccgcta ttccaaatgg agaggcgatc cgtgatgttc
      421 caaaaagcag gacggcgaat gcgatgagag ccaccaaggt cacccagctt cgagcgaatg
      481 aacgccctgt cgcatacagg acatgtcgat acagctgaag cgtggctgaa acgaggttga
      541 ccatgccggt gcgtacccta atcgggattg tgcaagaggt caagcgtcgg caatagatat
      601 agattcttgg ttgacttatc tcgatccgat gattatcaga tgcctcatca atagcctctt
      661 ttcacggagc gagtcatcat ggatgcaaac cgaatgacga tcaagctgca agaggcactc
      721 cagtccgcct ctgcccatgc gcagcgaaga agccatcagg gcatcgatgt tgagcacgtg
      781 ctgttggcac tgctcgacca ggaaggcgga acgactcttg ccctgctgga gggggccggt
      841 ctcgccttgc ctgctgttcg gcaagtagtc gaacaggcct tggcaaaact gccgcaagtg
      901 caaggttccg gggccgctcc tggtcaaatg catatcaccc ctcgcctaac ccaagtcctc
      961 aaccgtgccg aggatgaaca gaaatcgctc aaggacgact ttctcagcgt tgaacatgtg
     1021 ctactggcca tggttcaaga aggaggagtg ctcaagaaac tgggactcac cagagaccgt
     1081 ctcttggccg gattgcaaca ggtgcgcggc aatcaacgtg tcaccagtca ggatcccgaa
     1141 agtacctacc agtcgctggt gaaatacggg cgtgatctga ctcaattggc cggacaaggc
     1201 aagctcgacc ccgtcatcgg tcgtgacgac gaaatcaggc gtgtgattca gattctgtcc
     1261 cgccgaacga agaataaccc tgtgctcatc ggcgaaccgg gagtcggaaa aacagccatc
     1321 gtggaagggc tggcgattcg tatcgtcaaa ggggatgttc ccgaaagtct caaacagaag
     1381 aagctcttcg cgttggacat gggatcgctg gtcgccgggg ccaagtttcg cggcgagttc
     1441 gaggagcggc tcaaagccgt gctcaaggag attcaatcct cccaaggtca gattctgctc
     1501 ttcatcgacg aattgcatac ggtcgtcggt gcaggggcgg ccgaaggcgc gatggacgca
     1561 gccaatctgt tgaagccgat gctagcgagg ggtgaactgc acctcatcgg cgcgacgaca
     1621 ctcgatgaat accggaaaca catcgaaaaa gacgccgcat tggaacgccg cttccagacg
     1681 gtcctcgtcg accagccgtc cgtggaaaac accatttcca ttttgcgcgg actcaaagag
     1741 cgatatgaag tccaccatgg cgtgcggatc aaggacagcg ccctggtggc ggcagcgaaa
     1801 ttatcgcatc gatacatttc cgatcggttt cttccggata aagccatcga cttggttgat
     1861 gaagccgccg cgcggttgag gactgaaatc gacagccttc cggctgagct ggatgaggtc
     1921 tctcggaagg tgcttcagct ggaaatcgaa cgggaagctt tgaaaaaaga aaag
//
LOCUS       DASTWE010000987         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1286586, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000987 DASTWE010000000
VERSION     DASTWE010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286586"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFT30_10315"
     CDS             complement(<1..682)
                     /locus_tag="VFT30_10315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5093071.1"
                     /translation="MHTYYPLCAFENLQIEQTGNEVIVFDSKAQTFHLLNTTAHRILK
                     ACNGSNSIRDIADLLSREFGTDDLDSLVTDVTETIKSFESKGLMWFVRDETQGYPIDS
                     DQLTDSPLVAISVTGGSMFPVLFSGDKVLVKKSSLEDLNIGDIIVWVDHSNARVAHRI
                     VSVNTSSTPALITTKADVNFEPDPPVGFNRVLGKVVAVLQDGEPKWIKELGKTNGGSA
                     DQNGSSERA"
BASE COUNT          268 a          329 c          300 g          318 t
ORIGIN      
        1 cggcacgctc ggacgatcca ttctgatccg cagagcctcc gttagttttt ccgagttcct
       61 tgatccattt gggttcgccg tcctgaagca cagccacgac cttgcctaag acgcgattaa
      121 atcctaccgg cgggtctggc tcgaagttca catctgcttt ggttgtaatc agcgcgggcg
      181 tcgaggatgt gtttaccgaa actatccgat gggccacacg tgcattggaa tgatccaccc
      241 aaacgatgat gtctccgata ttcagatctt cgagtgagct tttcttgacc agcactttgt
      301 ctcccgaaaa gagcacagga aacatgctgc ctccggtcac ggaaatcgca actagcggac
      361 tatcggtcaa ctgatctgaa tcgatcggat atccctgtgt ttcgtccctc acaaaccaca
      421 tcaatccctt ggactcaaat gatttgatgg tctctgtcac atctgtaacg agtgagtcga
      481 gatcgtcggt accaaactca cgggaaagca gatcggcaat gtccctgatg ctattcgagc
      541 cattgcacgc cttgagaatg cgatgtgcgg tggtattcaa cagatgaaat gtttgcgcct
      601 tggagtcgaa tacgatgact tcgtttccag tctgctcgat ttgcaagttc tcgaaggcac
      661 agagtggata atatgtgtgc atcggcgaat cctcagacta cgcgccggtg atgtggtcgc
      721 tgacgacgcg ggctggaacc ggaataaacc gggtgttaac catccggctt attccggcgt
      781 tggttaagca gctactgtca ctgccattga cattgacatt gacactgaca ttgacactga
      841 cattgacatc ccccgccctc gccggggtcc tccggtagga tttcgccctg gagcatcgca
      901 acgtacctgg cgttttcgag ttcagttact gtcagactga caatggaagg tctttcgtag
      961 cgcatgattt gtcacctcct ttcgtaaggt ttgaggaagt ccaaagttgg acttaatcat
     1021 ccatcgacgc agatgattcc catatcggca gccatatcaa ctcttcaccg tgcgaatctc
     1081 caatgcgtgc gcgtgcgggc attctgcctg ttcgcacatc gctccaggtg ttcctgatgt
     1141 tggtcctaac catctggcac caccatcgtc tgatgtactt gtcagagttt tgcgatcgcg
     1201 gttcacatcc accgc
//
LOCUS       DASTWE010000988         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_705280, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000988 DASTWE010000000
VERSION     DASTWE010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705280"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFT30_10320"
     CDS             <1..934
                     /locus_tag="VFT30_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEU5093072.1"
                     /translation="KCEEVEVPLLFDTEIVKGLVDTTWHGRANRLSNEHEVHWDVIDD
                     TAAASRKTSHEHMSVMIPISVTPHVSSPTSHETQSAGHIIRQRRSAVAFDGKTSISSA
                     TFFHMMRRVMPWAERPQLERPMPWDVWPYDPTIHLLIFVHRVDGLTPGLYFLVRDRRK
                     LPFIQQTMNPELSWTTAPDCPDGLPLYWLLEGDAKKLAAQVSCHQDIAGDSVFSFGML
                     AEFEGTLRERGAWWYPRMFWEAGLLGQVLYLEAEAVGVRATGIGCFFDDPVHEIVGIQ
                     SLAIQSLYHFTIGGPVEDRRLQTLPPYHHLLSHS"
BASE COUNT          225 a          249 c          306 g          262 t
ORIGIN      
        1 gaaatgtgaa gaagtggagg tgccgctgct tttcgatacg gagatcgtca aagggctggt
       61 cgacacaact tggcatggga gagcgaatcg gttgagtaac gagcacgagg tccattggga
      121 cgtcatcgat gacactgcag ccgcgagccg gaaaacatca cacgagcaca tgtctgttat
      181 gattcccatt tctgttactc ctcacgtttc atctcctacg tctcatgaga ctcagtcggc
      241 cggtcacatc attcgtcagc gtcggagcgc tgttgcgttt gatggcaaga cgtcgatttc
      301 ttctgcgacg tttttccata tgatgcgacg agtcatgccg tgggctgaac gtccgcagtt
      361 ggaacggccg atgccatggg atgtgtggcc ctatgatcca acaattcatc tcctgatatt
      421 cgtccatcga gtcgatgggc ttacgccggg attatatttt ctcgtgcggg ataggagaaa
      481 gttgccgttc attcaacaga ccatgaatcc cgaattgagc tggaccaccg cgccggactg
      541 cccggatggg cttcctcttt attggttact tgaaggtgat gcaaaaaagc tggcggcgca
      601 ggtcagttgt catcaggaca ttgccggcga cagtgtcttc tcgttcggca tgttggccga
      661 gttcgagggg accttgcggg aacgaggcgc ctggtggtat ccacgcatgt tttgggaggc
      721 aggattgctc gggcaagtgt tatatctgga agcggaagcg gtaggagtac gggcgacggg
      781 aatcggctgc tttttcgatg atccggtcca tgagatcgtc ggcattcaga gcttagccat
      841 tcaatccctc tatcacttca cgattggtgg gccggtggaa gatcggcgct tgcagacctt
      901 accgccgtac catcatctgc tgtctcattc ataagacttg ggtgtccgtg aggtagttca
      961 tgttcaagat caagaagaag agcgacaagc cgaagcgtgt ggtgatctat aacatcgtcg
     1021 aggactattc tgagttcgac gc
//
LOCUS       DASTWE010000989         1026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_220175, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000989 DASTWE010000000
VERSION     DASTWE010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220175"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /gene="tatC"
                     /locus_tag="VFT30_10325"
     CDS             <1..607
                     /gene="tatC"
                     /locus_tag="VFT30_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5093073.1"
                     /translation="PTEALFASIKVSFLAAVLASLPVVFYQLWKFVQPALLPKEQRWG
                     IPILALAGVLFALGLLFCNLVILPLVIDFFVSFGMDRDITPELSVGTYIDFNVKFLLT
                     FGCAFELPLVLTILARVGVVTAGQLAHYRKHAILSALIISAVVTPDATLFTMMLMAVP
                     LMVLYEIGILGARLFGRNQPPDMNLPLDPDLPMGTAGHRVR"
     gene            616..>1026
                     /locus_tag="VFT30_10330"
     CDS             616..>1026
                     /locus_tag="VFT30_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093074.1"
                     /translation="MMNRSLLSLAMTLLTLSPVGSAVAQDPSSSPFESQAASIQALTM
                     VGSDILYAGSFGLGVFRSEDRGATWTKSSEGVSDPFILSLATAKDGTVYVGTFRGGVF
                     RTTDQGRTWHPVNAGLKRKEVKTLLAAGDLLYAGT"
BASE COUNT          176 a          302 c          286 g          262 t
ORIGIN      
        1 cccgacggaa gccttgttcg cttccatcaa agtttctttc cttgcggcgg tgctggctag
       61 cctccctgtc gtcttttatc agttgtggaa gttcgtccaa ccagcgctgc tcccgaagga
      121 gcagcgctgg ggcatcccca ttcttgcgct ggccggggtg ctctttgccc tcggccttct
      181 tttttgcaac cttgtcatcc ttcccctcgt catcgacttt ttcgtcagct tcggcatgga
      241 ccgcgacatt acgccggagc tgagcgtcgg cacgtacatt gattttaatg tcaaatttct
      301 gctgaccttt ggatgtgcat tcgaattgcc cctcgtcttg accattctcg ctcgtgtcgg
      361 cgtcgtcacg gcggggcagt tggctcacta tcgcaagcac gccatcttga gcgccttgat
      421 catctccgct gtggtgactc ccgatgccac gctcttcacc atgatgctca tggccgtgcc
      481 cttgatggtc ctctatgaaa ttggcatcct cggcgcccgt ctgttcggcc gcaaccagcc
      541 tccagacatg aatcttccac tggatcctga cctacccatg gggacagcgg gccatcgggt
      601 tcggtaagat acgtcatgat gaaccgatca ttgctttctc ttgccatgac cctcctgacc
      661 ctgtcgccgg tcggttccgc cgtcgctcag gacccgtcat cgagcccctt cgaatcgcaa
      721 gcggccagta ttcaggcgct cacgatggtg ggttcggata tcctctatgc cggctcattt
      781 gggcttggcg tctttcgaag cgaggatcgc ggagcgacct ggacgaaatc cagtgaaggg
      841 gtgagtgacc cgttcatatt gtcgctcgca acggcaaaag acggaacggt gtatgtggga
      901 acttttcggg gaggagtgtt tcgaaccacg gatcaaggta ggacctggca tccggtcaac
      961 gccggattga agcgcaaaga agtgaagacg ttgttggcgg cgggcgatct gctgtatgcg
     1021 gggact
//
LOCUS       DASTWE010000990         4752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_607745, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000990 DASTWE010000000
VERSION     DASTWE010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607745"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1217
                     /locus_tag="VFT30_10335"
     CDS             246..1217
                     /locus_tag="VFT30_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEU5093075.1"
                     /translation="MTNQQPMQGVSASAAATLPGPSTIKDSPAVERALNRSKIYLLIS
                     WSLLYPEDEEFLDYLRCGEFVADGRAALDALEVALGTDGGDRARARLISLRKQLASVE
                     SLIASECVNWQLSDLQSEHRRVFSHVITLDCPPYETLFGNDHVFAQSHVMGDISGFYK
                     AFGVELSKDIHERLDHLSVEFEFMHFLAYKESYSLCHDGPEKTQIVIDAQKKFVKNHI
                     GRWVPLFCRMLTKKADSGLFKLVADMTADWMEFETAFLGVTPQPYTETDYRPATFSSP
                     EGQTYECGAQDQGNELTMLLNEVGAQSFMDVKEKDKENEEGRPSGTA"
     gene            1286..2236
                     /locus_tag="VFT30_10340"
     CDS             1286..2236
                     /locus_tag="VFT30_10340"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEU5093076.1"
                     /translation="MRVAQTTNKKLVFAILLSALSVGLMLTLGRVPLAVSQPVTIPAK
                     TVKGPIPMDGANPVWESVPGVIVPLSGQLITTPMHPNISVKSVFVKAMTNGKEVGLRL
                     EWVDQTKNDTTIGPQDFRDQVAVMFPVNTAGAPPFQCMGQSGGTTNIWRWNAEWQKDL
                     GKDSAGIWDVDDQYPGIFWDYYFEEPAGGVTYPDRIGRSLGPFNSGIWSGNIMSDPTL
                     RVSSVEDLSANGFSTLTTQAHQDVIGNGVWEPSGSIKGGGYTGPTWRVVVKRTLENGD
                     ANDVQFKAGMSVPIAFAVWDGANIERNGMKSLSTWFTLKL"
     gene            2471..4249
                     /locus_tag="VFT30_10345"
     CDS             2471..4249
                     /locus_tag="VFT30_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5093077.1"
                     /translation="MKISLLFPPTWHPSQPYLSLPSLTGFLRQGGISDVSQRDLGIEL
                     LDTVLTKDYAAEVYQHLIAKQRELERAQTGETGPGSREHYVKVTDSLDRFSYLVDRIE
                     LAKETLRSEGFYDPDAYRASLFMIDKWLEVVSAVYFPTRLTVVDNQFGNYSIYSSKDL
                     MKVVRDESQNPYLSLFRDRFVSSIVNSRPDLIGVSITATSQIIPGLTLCRLIKEAAPD
                     LHVTIGGSIFTRLVDNIRRCPSLFELTDDIVVFEGETALLELVNQLAGKKDYSKVPNL
                     IYRHNGKITVNQPFYSENVNQLPAPNYDGFPLDRYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQKDPGRTVEELLWLKQRYGARHFFFTDECFALSPTKRLCQQMIEQQLD
                     VKWTCEMRFEKNLSRELLASMRDAGCLKIVFGLESFNQRIMDFMKKGIKQEWVRRIAD
                     DCVELGIAVHCYIIVGFPTEKEEEALETMNFIVGNKKLHESYGFSCQPCLFDLEKEAP
                     IMSDPGGYGIRRIMRPSAEDLSLGFFYEVQEGMTPDEAERLYQYVYERISEVVCELPF
                     NYAMADGLLYISRAKEGAGQAPMAAH"
     gene            4297..>4752
                     /locus_tag="VFT30_10350"
     CDS             4297..>4752
                     /locus_tag="VFT30_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093078.1"
                     /translation="MKAAALSERVTGKVSDFGPLFEYAVKLVLLTSFLELVLYRLASR
                     LGMHLSKMAADHPWITPTFTALTEVGTWLLNIVAVLLFLVLTLTMINRWGGPETGRLG
                     KLGSVGTALLLLLTVVFLVVQPGMLGAIVYNGLTLLVLTAFVSEYVVTHR"
BASE COUNT         1086 a         1190 c         1341 g         1135 t
ORIGIN      
        1 tgtggaatgt gtgagatggt ctgtaagact gtcaacgacc atgttgcaat ccgtgtagtg
       61 ccgtcgaggc agctggcaag gattgattca tgaaggtgtc accatggcca gatcgctcag
      121 aaaaactcaa ttcgcaacat caaaccaatt cacccttgac ttcgtaccgc ccttttctat
      181 acatactggc tctgttgcct aaccttggtg agtagggtga gcgagccggt caggagacga
      241 tcgctatgac gaatcaacag ccgatgcaag gcgtatcagc atctgcagcc gccacgttgc
      301 cgggcccttc gaccatcaaa gattctcctg ccgtcgaacg cgcgctgaac cgcagtaaaa
      361 tctatttgct gatctcctgg agtcttctct accccgagga cgaggaattt ctcgattatc
      421 tccggtgcgg cgagttcgta gcggatggtc gggctgctct cgatgcattg gaagtcgccc
      481 taggaaccga tggtggtgac cgagccaggg caaggctgat ctcgctcagg aagcagttgg
      541 cctcggtaga gagcttgatt gcctccgaat gcgttaactg gcagctcagc gatcttcagt
      601 ccgaacatcg gcgtgtgttc agtcacgtga ttacgctcga ttgccctccc tatgaaactc
      661 ttttcgggaa tgaccacgtg tttgcgcaat ctcatgtgat gggcgatatc tccggcttct
      721 ataaagcctt tggagttgaa ttgtcgaaag atattcacga gcgtctcgat cacctcagcg
      781 ttgaattcga attcatgcac tttctcgcct acaaggagtc ctactcgctc tgtcacgatg
      841 ggccggagaa aacgcagatc gtgatcgacg cacagaagaa attcgtcaag aatcatattg
      901 gccgatgggt accgctgttt tgtcgcatgc tgaccaagaa agcggactcc ggtctcttta
      961 aactagtggc cgatatgacc gctgattgga tggagtttga gacggccttc ttgggcgtga
     1021 cccctcagcc ctacacggaa accgattatc gtccggcgac gttcagctct cccgaaggcc
     1081 agacctatga atgcggtgcg caagatcagg ggaacgaatt gaccatgttg ttgaacgagg
     1141 tcggcgcgca atcgtttatg gatgtgaaag agaaagacaa agagaatgaa gaaggacgac
     1201 cttccggaac cgcatgaatg agttcttggt aacggttcta gcacaaacgc agtttttata
     1261 ataattcaga ggagggagta gcactatgag ggtagcgcag acgaccaaca agaaattggt
     1321 gtttgccatt cttctctccg ccctctccgt cggcctgatg ctgacgttgg ggcgagtacc
     1381 actggccgtc agtcaaccgg tgacgatacc ggccaagacg gtcaagggcc caatccccat
     1441 ggacggcgcc aatcccgttt gggaaagtgt tccgggcgtc atcgttccgt tgagcggtca
     1501 gttgattacg acaccgatgc acccgaacat ctcggtaaag tcggtattcg tcaaggccat
     1561 gaccaacggg aaagaggttg ggctgcgctt ggagtgggtt gatcaaacga agaatgacac
     1621 cacgataggc ccgcaagact tcagggacca ggttgcggtg atgtttccgg tgaatacggc
     1681 cggggctcca ccgttccagt gcatgggaca gtccggcggg acgaccaaca tctggcggtg
     1741 gaatgccgaa tggcagaagg acctgggcaa ggacagcgcg ggaatctggg atgttgacga
     1801 tcagtatccc ggcattttct gggattacta ctttgaagag ccggcggggg gtgtcaccta
     1861 tccagaccgt attggtcgga gtcttggacc gttcaactct ggtatctggt ccgggaacat
     1921 tatgtctgat ccgacacttc gcgtgagttc cgttgaagat ttgagcgcga acggcttcag
     1981 cacgctcacg acgcaagcgc accaggatgt cataggaaat ggagtctggg agccgtcggg
     2041 gtccatcaaa ggcggagggt ataccggccc gacttggcgg gtggtcgtga agcgaacctt
     2101 ggaaaacggt gatgcgaacg atgtccagtt caaggcagga atgtcggtgc cgatcgcatt
     2161 tgcagtatgg gacggtgcca acatcgagcg gaacggcatg aagtcgcttt cgacctggtt
     2221 cacattgaag ctgtagtgac ctgagcggca gtcgagctga tagcaacaac cgccgcgttg
     2281 cgttattcta aagaggcctc ggatcgggat cccagtaaat gagggaacgg ttcgaggcct
     2341 ttttcatgaa aaattcggca caggcttacg tctctatgcc gacagggttg catttcgtaa
     2401 agagcggagg atataagagg aaagatggct cgaacttgct gattttcaag cataaaagga
     2461 caagcttcgc atgaaaatct cgttactgtt ccccccgacc tggcatcctt ctcaacccta
     2521 tctcagttta ccgtccctga cggggttttt gcgtcagggt gggatttccg acgtgtcgca
     2581 gcgcgacctt ggtatcgaat tgttggatac tgtgctgact aaagattatg cagccgaggt
     2641 gtaccagcat ttgattgcca agcagcgcga gcttgagcgg gctcagactg gggagacggg
     2701 tccgggaagt cgcgagcatt acgtgaaggt gaccgactcg ctcgatcgct tctcttatct
     2761 cgttgatcgt atcgagcttg ccaaggaaac attgcgcagc gaaggatttt acgatcctga
     2821 cgcctatcga gcgagtttgt tcatgatcga caagtggttg gaagtcgttt ccgcggtgta
     2881 cttcccaacc cggcttaccg ttgtggataa tcaattcggc aactactcga tctattcttc
     2941 caaggacctg atgaaggtcg ttcgtgacga aagccagaac ccttacctga gtcttttcag
     3001 agacaggttc gtttcttcga tcgtcaatag ccgtcccgat cttatcgggg tctcgattac
     3061 ggccacatcc caaattatcc ctggcctcac tctctgtcga ctgattaagg aagctgctcc
     3121 ggaccttcac gtgaccatcg gtggcagcat ctttacccgt ctcgtggaca acattcgtcg
     3181 ctgcccgagc ctctttgagt tgaccgacga tatcgtcgtg tttgagggcg aaacggcctt
     3241 gctggaattg gtcaatcagc tggcaggcaa gaaggactac agcaaggttc ccaacctgat
     3301 ttatcgccac aatgggaaga tcaccgtcaa ccagccgttc tattcagaaa atgtcaatca
     3361 actcccggcg ccgaactatg acggattccc gctggatcgc tacttgtcgc cggagccagt
     3421 cctgccggtc cagttttcgc ggggctgtta ctacaaagat tgtgcgttct gtgcgctgac
     3481 cctcgatcac cagaatttca gacagaaaga cccggggcgt acggtcgagg agctgctatg
     3541 gctgaaacaa cgctatggcg cgcgccattt cttcttcacc gacgaatgtt ttgcgctatc
     3601 gccgacaaaa cggttgtgtc agcagatgat cgagcaacag ctcgatgtca aatggacgtg
     3661 cgagatgcgg ttcgagaaaa atctctcacg cgagctgttg gcgtcaatgc gggatgccgg
     3721 gtgtctgaag atcgtctttg gattggagtc cttcaatcaa cgtatcatgg atttcatgaa
     3781 gaagggcatc aagcaagaat gggtccggcg gatcgcggat gactgtgtgg aactcggcat
     3841 cgccgtgcat tgttacatca tcgtcggatt ccccacggaa aaagaagaag aagcgcttga
     3901 aaccatgaac ttcatcgtcg gaaacaagaa actccacgag tcgtatggat tttcttgtca
     3961 gccctgcctg ttcgatttgg aaaaggaagc tcctattatg agcgaccccg gcggatatgg
     4021 aattcgacgt atcatgcgac cgtccgcgga agacttgagc ctcggatttt tctatgaagt
     4081 gcaagagggc atgactcccg acgaggcaga gcgactgtat cagtatgtgt atgagcggat
     4141 cagtgaagtc gtgtgcgagc ttcccttcaa ctatgcaatg gcggatgggt tactctacat
     4201 ctcaagggcg aaagaaggag ccggacaggc tccgatggcc gctcattgac ctcatttttt
     4261 tgcgacagct ataatgtcag atcttatggt gaggtcatga aggcagcggc tcttagcgag
     4321 cgggtaacag ggaaagtttc agacttcggt ccactgttcg agtatgccgt caaactcgtt
     4381 ctcctgacct cttttcttga gctggttctc tatcgtctgg cttcccgtct cgggatgcat
     4441 ctcagcaaga tggcggcaga tcacccgtgg atcacgccca cattcaccgc cctgaccgag
     4501 gtgggcacat ggcttcttaa cattgtcgcc gtgctgctgt tcttggtgct cacgttgacc
     4561 atgatcaatc gatggggagg acctgaaaca ggtcggctcg gcaaacttgg tagtgtaggg
     4621 acggcactct tgttgctgtt gaccgtagta tttctcgttg tgcagcccgg catgctgggc
     4681 gccatcgtct acaacgggtt gacgctgctg gtgctaacgg catttgtgtc ggaatatgtg
     4741 gtgacccatc gt
//
LOCUS       DASTWE010000991         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_996381, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000991 DASTWE010000000
VERSION     DASTWE010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996381"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VFT30_10355"
     CDS             complement(<1..1483)
                     /locus_tag="VFT30_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5093079.1"
                     /translation="MLRLNDALRNVSGVGQTDPFNAAVSIRGFLTTNNFFKNGFRDDG
                     NFASIAPRDTLNIESIEVVKGPPSFLYGRADPGGIINQITKSPLRNPYYSAEMILGNY
                     DLYRPQMDIGGPLNASKTLTYRFNAMYESAGSYREGVRSQRIFVAPTIGWEAGPRTTF
                     RFEGEYFYDNSVVDRGLVAIGGAVAPIPITRFLGDPTRKNETHHGKATLTFLHDFNDI
                     FRWRTAFRAAVTRGLYSSRESWFLVGNETDGILNLAQFRIPTTSQSHILQNELHGNFT
                     TGPIKHKTIVGVELGREVESESVASDNPNQDTSIPGQFSFINIFNPVDRLFLNTPLVK
                     FSNTRVVNNFLGFYFGDHITLLDNLHLHGGGRFDYFKQHLTNGPTDDNPTQTQDSQTD
                     LAFSPSIGLTYQPWKPIALFANYTESFAPQQPGSRTFEGTLIDPERGKAYEGGIKFQF
                     FENRLRAKVAGFRIKKTNVLTLDPINGFPFSVPTGEQRSQGIEF"
BASE COUNT          341 a          375 c          407 g          381 t
ORIGIN      
        1 cgaattcgat gccctggctg cgctgttcac ccgttggcac ggaaaagggg aatccgttaa
       61 taggatcgag cgtcaaaaca ttcgtttttt tgatacgaaa gccggcgact ttcgcgcgca
      121 accggttctc gaagaattgg aatttgatgc cgccttcata ggccttgcct cgctcgggat
      181 cgatcagcgt accctcaaag gttctgcttc caggttgttg cggggcaaac gactcagtgt
      241 aattggcgaa gagcgcgatc ggtttccatg gctgataggt cagtccgatc gatgggctga
      301 aggcgaggtc ggtttggcta tcctgggtct gagtcggatt gtcatcggtc ggaccattcg
      361 tgagatgttg cttaaagtaa tcgaatcgac cacctccatg cagatggaga ttatcgagaa
      421 gggttatgtg gtcaccaaaa tagaagccga ggaaattatt gactactctg gtattgctga
      481 atttgacgag cggcgtattc aaaaagagtc ggtccacagg attgaaaata ttgataaagc
      541 tgaattggcc cggtatgctg gtatcctgat tggggttatc gctggctact gattccgact
      601 cgacttcgcg tcccagttcg accccgacga tggttttgtg cttgatcggg ccagtagtaa
      661 agttcccgtg taattcgttt tgcaagatgt ggctctgtga agtcgtcgga atgcgaaact
      721 gcgccagatt cagaatcccg tcggtttcat tcccgaccag aaaccatgat tctcggcttg
      781 aatacagacc acgcgtcacg gcggctctga acgccgtgcg ccatctgaag atgtcattga
      841 agtcgtgaag gaatgtcagc gtggcctttc catgatgggt ttcatttttt ctcgtcggat
      901 cgcctaggaa ccgggtgata ggaatcggcg ccactgctcc gccgattgca acgagaccac
      961 gatcgactac gctattgtca tagaaatatt ccccctcgaa ccggaaggtc gtcctcggtc
     1021 ccgcctccca tccaatagtg ggcgcgacaa agatccgttg agaacgcact ccctcacgat
     1081 agctgcctgc ggattcgtac atagcgttga accgataggt cagtgtcttg cttgcgttca
     1141 gcgggccacc gatatccatt tgcggacgat agagatcgta gttgccgagg atcatttccg
     1201 ccgaatagta cggattccga agcggcgatt ttgtaatctg attaatgatt cctccaggat
     1261 cggcccggcc atacagaaac gacggtgggc ctttcaccac ctcgattgac tcgatgttga
     1321 gagtatcccg cggcgcgatc gaagcgaaat tcccgtcgtc gcgaaaccca ttcttaaaaa
     1381 agttattggt tgtcagaaat cctcggattg agacggctgc attgaaggga tcggtttgtc
     1441 ccacaccgct cacattccgc agcgcatcat tcaggcggag caccttctga tcttcgatga
     1501 cttg
//
LOCUS       DASTWE010000992         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_317079, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000992 DASTWE010000000
VERSION     DASTWE010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317079"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /gene="glmM"
                     /locus_tag="VFT30_10360"
     CDS             complement(<1..332)
                     /gene="glmM"
                     /locus_tag="VFT30_10360"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448722.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5093080.1"
                     /translation="MMRKLFGTDGVRGVANLDPMTSEMAMQLGRAAAHIFMRRAGRHQ
                     IVIGKDTRISGYMLESALMAGICSMGVDVLVVGPMPTPAIAFLTRSLRADAGVVISAS
                     HNPYQDNG"
     gene            complement(322..1248)
                     /gene="folP"
                     /locus_tag="VFT30_10365"
     CDS             complement(322..1248)
                     /gene="folP"
                     /locus_tag="VFT30_10365"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5093081.1"
                     /translation="MDFPFIGGAMGLVSPQVITAQHWFSAKGREIHCKGRPLIMGIVN
                     ITTDSFYDGGRYVEPERAIARALELVEQGADIIDVGGESTRPGAGSVSEQDELARVIP
                     VVKGLAHRVPVPISIDTTKSRVAEVALDCGASIINDVSALRTDSAMASVIAQCYAGVV
                     LMHMQGSPQTMQQSPHYSDVVGEVVHFLDERLQIALGAGIARTNIILDPGLGFGKLLV
                     HNLNLLDGLSALGALNRPVLVGLSRKAFIGKVVERPVEYREWGTAAAVALAVDRGAHI
                     VRVHDVAMMIDVVKMAAALNPRWSSCGQEHDA"
     gene            complement(1354..1521)
                     /locus_tag="VFT30_10370"
     CDS             complement(1354..1521)
                     /locus_tag="VFT30_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093082.1"
                     /translation="MKRFVTENYERAKRVLTEQMTSLKALAEALLEKEVLDAPEIDQI
                     LLQSSSQTVPA"
BASE COUNT          380 a          454 c          357 g          333 t
ORIGIN      
        1 attccgttgt cttgataagg attgtgcgat gccgaaatga ccacccctgc atccgcccgt
       61 aggctcctgg tcaaaaaggc gatcgctggc gtgggcatcg gtccgactac cagcacatcc
      121 acccccatcg agcagattcc ggccatcaag gcagactcca gcatatatcc ggagatacgg
      181 gtgtctttac cgatgacgat ctgatgacgg cccgctcgtc gcataaagat atgtgcggcg
      241 gctcgtccga gctgcatcgc catttcactg gtcatgggat caaggttggc aacccctcgg
      301 acgccgtcgg ttccaaataa tttacgcatc atgctcctgt ccgcaagatg accagcgtgg
      361 attcaacgcc gcagccatct tgactacgtc gatcatcata gccacatcat ggacacgcac
      421 aatgtgggcg ccgcgatcga ccgccaatgc caccgccgct gcggttcccc attctcgata
      481 ctcaacaggc cgctcaacca ctttcccaat aaacgccttc cgcgacaggc cgaccaatac
      541 aggacggttc aatgctccta aagcagacag cccatcaaga aggttgagat tatggaccaa
      601 cagcttacca aaacctaagc ccggatcaag aatgatgttt gttcgagcaa tgcctgcacc
      661 gagagcaatc tgcaaacgct catcaagaaa gtgcactact tcgccgacca cgtcggaata
      721 atgcggtgat tgttgcatcg tttgcggaga tccttgcata tgcatcagga cgactcccgc
      781 atagcattgg gcaatgaccg aagccattgc agaatccgtc cgcaaggcac ttacgtcgtt
      841 gatgatcgag gctccgcaat ccagagcgac ttctgcgact cgagacttag tggtatcgat
      901 cgaaatcggg accggtacac gatgtgccaa tcccttcacc accgggatca cacgagccaa
      961 ttcatcctgt tcactaacag aacctgcgcc aggccgtgtc gactctcccc caacatcgat
     1021 gatgtccgct ccttgttcaa caagttccag cgcacgtgca atggcccgtt caggttcaac
     1081 atatcgtcca ccatcataga aggaatccgt cgtgatgttt acgatgccca tgataagcgg
     1141 ccgacctttg caatgaattt ctcgcccttt ggcagagaac caatgctgtg ccgttatgac
     1201 ctgtggtgac accaatccca ttgctccacc gataaaagga aaatccaaac cgttgctccg
     1261 gtcagcctcg aataaacaga cccagagcgg tctgtaaatg cagcacgacg gagacaacgg
     1321 ctccaacacc aacgaaccgt tggcaccatg tatttaggct ggaactgttt gagaggagga
     1381 ttgcaacaga atctgatcaa tttccggtgc gtcgagcact tctttttcaa gtagcgcctc
     1441 cgccaaggcc ttcagactcg tcatttgctc agtcagcaca cgtttggcac gttcataatt
     1501 ttctgtaacg aagcgcttga tctc
//
LOCUS       DASTWE010000993         1100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1416558, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000993 DASTWE010000000
VERSION     DASTWE010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416558"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..729)
                     /locus_tag="VFT30_10375"
     CDS             complement(85..729)
                     /locus_tag="VFT30_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093083.1"
                     /translation="MVSEVPYNFFMRRNLGILHGLLGTIVLGGWGCATELPSLVDQTQ
                     ITGSLVVGRVLTLLTGETSRRYAPAVRFFELEDQTSQKRFQVEIKSSDRYFAVDLPPG
                     RYRLTRVQISEGPFMSMADLSMAFLVDAGVTMYVGAWRFGVDSPRYGRMVVASAVTDQ
                     AETARVRDFLDDRYPTVNKRTMVEAIPQPAQIEARLYEVTPYPRYSRYFRRHWW"
BASE COUNT          295 a          296 c          275 g          234 t
ORIGIN      
        1 caaccggcca ccaaagtcat ggcgaggcac agcaccgtaa taggaatggc acagatttga
       61 gggatcttca tagcgacatg gcttctacca ccaatgcctg cgaaaatatc tcgaataccg
      121 cgggtaaggc gtgacctcat agagccgcgc ttcgatctgt gccggctgcg gaattgcttc
      181 aaccatggtg cgcttattga ctgtcggata ccgatcatcg agaaagtcgc gaacccgtgc
      241 tgtttctgcc tggtcagtaa cggcggaggc tacgaccatc cgcccatagc gcggtgaatc
      301 aactccgaat ctccaggccc ctacatacat agtgacaccg gcgtcaacaa gaaaggccat
      361 ggacaaatcg gccatggaca tgaacggccc ttcgctgatc tgcactcggg tgagtcgata
      421 ccttccaggc ggtaaatcaa cggcaaaata tcggtcgctg gacttgatct ctacctggaa
      481 acgtttttga gacgtctgat cttccaattc aaagaaacgc actgccggtg catagcgtcg
      541 ggatgtttct ccggtcaaga gcgtcaagac tcgccccacg accaagctgc cggtgatctg
      601 cgtctgatcg actaaagagg gtaattcagt cgcacaaccc catcccccca aaacgattgt
      661 tcccaagaga ccgtgcagaa tacccaggtt tctcctcatg aaaaaattat acggcacttc
      721 ggaaaccatg gggagtccag caccgactga aaattgacct tgacattgaa atgcggatcg
      781 agatattaat aatcgttctc aatttcatta catccaccaa ccatagcaat atggctattg
      841 caaaagctcc atctttggaa cgctgtcagg cgcttgaaac gcgctgcgag tgtgggcaac
      901 tgatcgccaa ggtctgtgaa caagggctag aactgaagtg caagcggtgt aaacgcattg
      961 tcgtcatccc cttctcgtca atcgagggct ggggggcggg aacgtcataa tccatcgggc
     1021 gaaaggagga actgtcatta catctcacgg atagaacgta gattcacgat tacctgaaga
     1081 ccagagacca tcgagtcccg
//
LOCUS       DASTWE010000994         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_640533, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000994 DASTWE010000000
VERSION     DASTWE010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640533"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFT30_10380"
     CDS             <1..313
                     /locus_tag="VFT30_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU5093084.1"
                     /translation="SVEIGNNCLIAANCQIFDSDAHDISFPDVENRINTRGKSRPIRI
                     EDNVWIGANSIILPGVTIGNGSVIAANSVVTKHVPPMVVAGGNPARIIKYCSHMEQSI
                     S"
     gene            complement(398..880)
                     /locus_tag="VFT30_10385"
     CDS             complement(398..880)
                     /locus_tag="VFT30_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093085.1"
                     /translation="MSGLMSADDIFSKAEAAAVAATGPDEKALQIDYDALREKFRKAL
                     GDRKVALCHVNRFLPEGYEDQGRFNLVLLTAGNVVFDIVIGDSYFRYDVVSVGQLDKV
                     QLIDAMWDNREKRREEPFLSLRLMHGEETHLLLALDDNERASLLKFASGVTAARNPER
                     "
     gene            complement(990..>1251)
                     /locus_tag="VFT30_10390"
     CDS             complement(990..>1251)
                     /locus_tag="VFT30_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093086.1"
                     /translation="VHWVQANAYDPNQDGVIDFHEYMVGPLSARPGRLTAASDELHPM
                     RLVNESGYQNFGLVRVTKDAFEVTVVDEAGAKRFSHVVPARP"
BASE COUNT          313 a          343 c          290 g          305 t
ORIGIN      
        1 atcagttgaa atcgggaata actgcttgat agcagcaaac tgccagattt ttgattctga
       61 tgcgcatgac atatcgtttc ccgatgttga gaatagaatt aatacaagag gtaaaagcag
      121 acctattagg atagaagaca atgtctggat cggagctaat tcgataatcc ttccaggtgt
      181 taccattgga aacggttcgg taattgctgc caatagtgtt gtcaccaagc atgtaccacc
      241 catggttgta gcagggggca atccagccag gattattaag tattgttcgc atatggagca
      301 atcaattagc tagactcgcc tgcgtagctc ttttgattgc cattcacgga gctgaaatat
      361 aatcacttcc ttagaaagga tgtgtgatcc cgatacctta gcgctcgggg ttccgcgccg
      421 ccgtgacccc tgaggcaaac ttcagcaaac tcgcccgttc attgtcgtca agcgccaaca
      481 gcagatgcgt ttcctccccg tgcatcaaac gaaggcttaa aaatggctct tctcgacgct
      541 tttctcggtt gtcccacatc gcatcgatca gctgcacctt gtccagttga ccgaccgaca
      601 caacatcgta cctgaaatag gaatcgccaa tgacgatgtc gaataccaca ttgcccgccg
      661 tcagcagcac caaattgaac cgaccttgat cctcgtaccc ttccggtaaa aacctattga
      721 cgtggcaaag cgccactttt cggtcgccca acgccttgcg aaatttctcc ctcaatgcgt
      781 cataatctat ttgcagggct ttttcatccg gcccggtcgc cgctacagcc gccgcttccg
      841 ctttggagaa aatatcatcg gcggacatca atcctgacat gacttgtgct ccctcttaat
      901 cgttgaagtg gatctgacca atgacctcgg gagtcgtacg gtaaccggcc atctgccgca
      961 attgcaaggg gcttgagcag tgagagcgtc tatggcctag ccggcacaac gtgagaaaat
     1021 cgcttggcac ccgcttcgtc taccacggtc acttcgaacg cgtccttcgt gacacggacc
     1081 aggccgaagt tctggtaccc cgactcattc accaatcgca tgggatgtag ttcatcgctg
     1141 gcagcagtaa gccttccagg cctggccgag aggggaccga ccatatactc atgaaaatca
     1201 atgaccccgt cttgattcgg atcataggca ttggcttgaa cccaatgcac a
//
LOCUS       DASTWE010000995         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_479238, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000995 DASTWE010000000
VERSION     DASTWE010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479238"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1724
                     /locus_tag="VFT30_10395"
     CDS             342..1724
                     /locus_tag="VFT30_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5093087.1"
                     /translation="MPTRFRIEDAALAGTLRQIEQLIRRAGGRTWLVGGSVRDLVLGL
                     QPRDLDIEVVGLQPGQVHALLTERFSVQFVGKAFQVFKLQGLPVDLSIPSRIPTEDIS
                     LPGLPKQADPGMPIDEALARRDFTINAMAWDPDTMELRDPFNGRGDLDSRILRHASAR
                     FAEDPLRVLRGMQLSARFELTVAPETVALCRTLTQEGQPSERLWEEWKTLLLQGRKPS
                     LGLHFLRHCGWLRFYPELAALQGCPQDPIWHPEGDVWIHTLHCLDWFATERTGHEADD
                     LVVGLGVLCHDFGKPSTTREEYGRVTSRGHESEGADPTRRFLERLTNRHDLIDEIILL
                     VCCHLRPRALYDAKASDSAVRRLAKQVHRIDRLVRVARADDAGRPPKQFDGFPAGEWL
                     LTKAQHLAVDRRAPLPIVMGRHLLELGVQPGPDMGRLLDDCYEAQLDGDFGTLEEGIA
                     YVKSRLSASR"
     gene            1813..2007
                     /locus_tag="VFT30_10400"
     CDS             1813..2007
                     /locus_tag="VFT30_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5093088.1"
                     /translation="MHVQLNHPSRSIEIKGPKRAKDLLRELNLLAEAHLVIRGDELVT
                     EDEMLSDQDKIEIRPVISGG"
BASE COUNT          494 a          647 c          603 g          539 t
ORIGIN      
        1 attcccgctg ttgcttggtt cgccaacgca ttctatttgc ttacatcatg gttgacatgc
       61 tccgctaaaa gaaagcaatt ttatgggtac tgcttccatt acagccggag ttgcgggact
      121 tggatatggt tcacacactc tattccagca gacgagagcg gtgtgttccc atgagctgct
      181 agtaggtctt atcactaggt ttcgctccgg atggaagcaa tggctattat cactcttgtc
      241 gcagcttttc attatcggca ggcaccgagg aaggcggcta tctgaatcgg ttcacgattg
      301 aataggggtt tgggtcactt tagttttcgc tcactgagct gatgcccact cgctttcgca
      361 tcgaagacgc cgcgcttgca ggaacactgc gccagattga acaactgatc cgccgtgccg
      421 gcgggcggac ttggctggtc ggtggcagtg tccgcgacct tgtgctcggt ctacagcctc
      481 gggatctgga catcgaagtc gttgggctcc agcccggtca agtccatgca ctgctgactg
      541 agcgcttttc cgtgcaattc gtcggaaaag cattccaggt cttcaagctg caaggcctcc
      601 ccgtcgatct ttccattcct tcccgcatac ccactgaaga catatccctc cccggcttgc
      661 cgaaacaagc cgatcccggc atgccgatcg acgaggcgct tgcacgacga gacttcacca
      721 taaacgcgat ggcctgggat ccggacacta tggaacttcg cgatcccttc aacggtcgcg
      781 gcgatcttga ttcacggata ttgcgccacg cgtcggctcg attcgccgaa gatcctctgc
      841 gcgtcttgcg tggtatgcaa ctgtctgccc gctttgagct caccgttgcg ccggaaacag
      901 tagccctgtg ccgaacactg acccaggagg gtcagccgag cgagcgtctg tgggaagaat
      961 ggaaaaccct actccttcaa gggcgcaagc cgtcgctggg attgcacttc ttacgtcact
     1021 gcggctggct ccgtttctat ccggagctcg ccgcccttca aggttgcccg caagatccca
     1081 tctggcaccc ggaaggcgac gtctggattc atacactcca ctgtctggac tggttcgcca
     1141 cagagcgaac cggtcatgaa gcggacgacc ttgtggtcgg cttaggcgtc ctctgccatg
     1201 acttcggcaa gccgtccacg accagagagg agtatgggcg cgtcacctcg cgaggacatg
     1261 aatcggaagg ggcagaccca acgagacgtt ttcttgagcg actgaccaac cggcacgacc
     1321 tcatcgatga gataatcctc ctcgtatgtt gccatcttcg tcctcgtgct ctctatgatg
     1381 ccaaagcttc ggacagcgcc gtccgtcgat tggcaaagca agtccaccgc atcgaccgat
     1441 tggtgcgcgt ggcacgggcc gacgatgcag gccgtccgcc gaaacagttt gatggatttc
     1501 cggccggcga atggctattg accaaggccc agcatctggc agtggaccgc cgggcccctc
     1561 ttcccattgt catgggccgt cacctattgg aattgggtgt ccagccaggt cctgatatgg
     1621 gccgactcct tgatgactgt tatgaggctc aacttgatgg agactttgga acgctggagg
     1681 aaggcatcgc ctatgtaaag agccgactct ctgcctcgcg ctaattagct tcttgctctc
     1741 tgaccttcga cgaacagtcg tcattcctga acacccttgc cccttggacg ctggaacgcg
     1801 tatcatcctc ccatgcatgt gcaattgaac cacccgtcgc gatcgatcga aatcaagggg
     1861 ccgaagcgag ccaaagacct gctgcgcgaa ttgaatcttt tggcggaagc gcatttggtc
     1921 attcgtggcg acgaattagt gactgaggat gagatgctct ctgaccaaga caagatcgaa
     1981 attcggccgg tgatttcggg tgggtaaggg ccatcccgat ttttcataat aaaacactgc
     2041 tttttcgatc aatcgatggc agagaactcg gcaaagcatc gcggcaggct gtagctcaga
     2101 acagtatgtc agcgccaaag acacttgaat ggtagaaata ggaacacgaa tatctctggt
     2161 ttctaccgaa cgttccaact aattcttcaa cattcttcaa tatcagtgtt cagatctatt
     2221 tcctatccaa aaccatgaca gcaataaaaa atgaggcggt ttagtcgtct tcatgccatt
     2281 ccg
//
LOCUS       DASTWE010000996         1316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1157846, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000996 DASTWE010000000
VERSION     DASTWE010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157846"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1316
                     /locus_tag="VFT30_10405"
     CDS             <1..>1316
                     /locus_tag="VFT30_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEU5093089.1"
                     /translation="SVGDQALMDDDYLDGQSGTDWLFGFLGDDVLMGGEGADYLLGDS
                     VPENSPAVQILYTSATFYYGLIAFEPSDYWFTTVEGGADYLDGGDGNDILRGDGGNDV
                     LLGGNQDDELIGDDGNATQVQQGDDMLDGGDGDDLLFGGGGADILSGGEDSDQLIGDY
                     LNASGLNEGQDILDGGGGNDFLFGGGGDDVLNGGSGSDELHGGVGLDTLFGGLENDQL
                     FGGEGLDELFGAEGNDVLAGGDGHDTLWGGDGDDQLRGEAGDDVLDGGTGSNTLLGEA
                     GNDHVFGGIGNDELFGGSGQDRIEGDVGIDYIEGGADNDVIYGGSGDDRIYGDANPNL
                     APPNSSPTGGDDVIDGGEGNDDLKGGLGNDILLGGVGNDLLNGGEGDDVLDGGVGNDT
                     LLAGGTGADVIHGGEGNDHLYAGTRPLNGGGGEGGGEGGEGFVDFS"
BASE COUNT          292 a          253 c          483 g          288 t
ORIGIN      
        1 aagcgtcggt gatcaagctc tcatggacga cgactatttg gatggccaga gcggaactga
       61 ctggctcttt gggtttttgg gagacgatgt actgatgggt ggcgaagggg cggactatct
      121 cttaggagat agcgtgcccg agaatagccc agctgtccaa attctctaca ccagtgcgac
      181 attttactat ggcctgattg ccttcgagcc ctcagattac tggttcacca cggttgaagg
      241 aggggcggac tacctcgatg gtggtgacgg gaacgacatt ctgcgagggg atggaggaaa
      301 cgacgttctg ctgggcggca atcaggatga cgaattgatt ggtgatgatg gtaacgccac
      361 tcaggttcaa cagggcgatg acatgctcga tggtggagac ggcgatgatc ttcttttcgg
      421 aggcggggga gctgatatct tgagcggagg cgaggatagc gatcagttga tcggggatta
      481 tctcaatgcg agcgggctca atgaaggcca ggacattctc gacggtggag gaggaaatga
      541 cttcctattt ggtggaggcg gggatgacgt tctgaatggt ggaagtggca gtgatgaact
      601 tcatggaggt gtcgggctcg atacgctctt tggaggattg gagaacgacc aactatttgg
      661 aggcgagggg ctcgacgaac tctttggagc cgagggaaat gatgtgcttg ccggcggcga
      721 cggccacgac accctgtggg gtggtgacgg tgatgaccag ctgaggggcg aggcgggcga
      781 cgatgtgctc gatggcggga ctggcagcaa taccctgctc ggagaggcag ggaacgacca
      841 tgtgtttggt ggaatcggta acgacgagct ctttggtgga tctggccagg atcgtatcga
      901 aggagacgtt ggcatcgact atatcgaagg cggtgcggac aacgatgtga tctatggtgg
      961 gtcgggtgat gatcggatct atggcgatgc taatcccaat cttgctccac caaatagttc
     1021 tccgactggt ggggatgatg tgattgatgg cggagaaggc aacgatgatc tcaaaggggg
     1081 attgggcaac gacatcttgc ttggtggggt gggtaatgat ctgctgaacg gaggagaagg
     1141 ggacgatgtc ctcgacggcg gtgttggaaa cgatacgctg ctggctggag ggactggtgc
     1201 cgatgtgatt cacggaggcg aggggaatga ccatctctat gcagggacac ggccgttgaa
     1261 cggaggaggc ggagagggag gtggtgaagg aggcgaagga tttgtggatt tttcgg
//
LOCUS       DASTWE010000997         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_414149, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000997 DASTWE010000000
VERSION     DASTWE010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414149"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1139
                     /locus_tag="VFT30_10410"
     CDS             <1..>1139
                     /locus_tag="VFT30_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5093090.1"
                     /translation="LEGARGTIVDRHGKILAMNVEVPSVFGVPNTLDSPLKTARQLSP
                     ILHVRTDELERKLRQDRSFVWLARKLDPEQGRRLDRLSLDGIGVVMEGRRFYPKGSLL
                     AHVLGFSGMDGEGLEGVEHRYESSLRGEKRMMVLQRDALGRTVFPKGMTERSPTPGHS
                     LTLTIDEVIQYIAERELEDAVSRAQAKSGTMIVLDPKTGAVLAMAVSPRFDPNAVSTL
                     SPDRWRNRALTDAYEPGSTMKAMMAAAAIEERVVRPNTMVFGEHGHMTVANTVIHDHE
                     RLGWVSFAQVIQKSSNIGAAKTGMALGEQRLYRYLQAFGFGQRTEIDLPGEGAGLVRN
                     PKDWGRRSVASISMGQEIGVTPLQMVSAGAAIANGGVLMKPYVVS"
BASE COUNT          258 a          249 c          374 g          258 t
ORIGIN      
        1 gctggaaggg gcgcgcggta cgattgtcga ccgacacggc aagattctgg ccatgaatgt
       61 agaagtaccg tccgtattcg gagtcccaaa cacattagat agtcctctga agactgctcg
      121 acagttgtcg ccgattttgc atgtgaggac cgatgagttg gaacgaaaac ttcgacagga
      181 ccggagtttc gtgtggttgg ctcgaaagtt ggatcctgag caagggcgtc gacttgatcg
      241 tttgtcgctt gatggaatcg gggttgtaat ggaagggagg cggttttatc ccaaggggtc
      301 gctcctagct catgtattgg gtttttcggg aatggacggt gaaggtttgg agggtgtaga
      361 gcatcgctat gaatcgtctc tccgtggtga gaagcggatg atggtgctgc agcgtgatgc
      421 cttggggcgt acggtgtttc ccaaaggaat gacggaacga agtccgacgc ccggccatag
      481 cctcacgctt acgatcgatg aagtgattca gtatatcgcg gaaagagagc ttgaagacgc
      541 ggtcagccgt gcacaggcaa agtcaggaac tatgattgtg cttgatccaa agaccggagc
      601 ggtgttggct atggcggtga gtccgcgatt cgaccccaat gccgtgtcga ctctcagtcc
      661 tgatcgctgg cgaaacaggg cgcttacaga tgcctatgag cccgggtcca ccatgaaggc
      721 aatgatggct gcggctgcca ttgaggaacg agtggtaagg ccgaatacaa tggtattcgg
      781 tgagcacggt catatgacgg tcgcgaacac cgtgattcat gatcatgagc gactgggatg
      841 ggtgtccttt gctcaggtga ttcagaagtc cagcaatatc ggagcggcga agaccggtat
      901 ggcgctaggg gaacagcggc tctatcgata tctccaggca ttcggtttcg gacagcgaac
      961 ggagatcgat cttccaggag agggggcagg attggttaga aatcccaaag actgggggcg
     1021 tcgttcggtc gcatccattt ccatggggca agagatcggt gtgacgccgc ttcaaatggt
     1081 atccgcgggt gcggccatcg caaatggagg cgtgttgatg aaaccttacg tggtttcag
//
LOCUS       DASTWE010000998         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_58916, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000998 DASTWE010000000
VERSION     DASTWE010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58916"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFT30_10415"
     CDS             <1..619
                     /locus_tag="VFT30_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093091.1"
                     /translation="LRWPMQSELVSASKTHETQLKAARAEEQPVYSMVASVLPLPVQR
                     RLNDYADYYDASYKAAGEGQYGSLPKWKNYIHFPASGLMDYVTNPIENIVGLQPLAPW
                     DLYNGFVVDTEAHLRLASLQAWLRRGPADGDLLTRIAKAGQSLYDPYTGLPMLVNMKK
                     GVLYSVGHDGKDQDADPSSDVVVEIPAIQMSAAQAKASAASSKSR"
     gene            721..>1013
                     /locus_tag="VFT30_10420"
     CDS             721..>1013
                     /locus_tag="VFT30_10420"
                     /inference="COORDINATES: protein motif:HMM:NF021997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5093092.1"
                     /translation="MEHSPRRFFNSLSVRKGATFPTAVPTQIVSNEEFLPIRQTAEQA
                     CVEKVTTELMEKAAARRGLTRRDFLKTTGGMAAALLAMNEAFGRFFNIGEIEL"
BASE COUNT          257 a          267 c          268 g          221 t
ORIGIN      
        1 gctgcgctgg cctatgcaaa gcgaactggt ctcggcctcg aagacgcacg agacccagct
       61 gaaggcagcg agagccgaag aacagccggt atattcaatg gtggcatcgg ttcttccctt
      121 gccggtacaa cgacgtctga acgattatgc agattattac gacgcgtctt acaaagctgc
      181 cggcgaaggg caatatggtt cgttgccaaa gtggaaaaac tatatccact ttccagcgtc
      241 aggcctgatg gactatgtca ctaatccgat cgagaatatt gtcggcctcc aaccgcttgc
      301 accatgggac ttatacaacg gattcgttgt cgatacggaa gcacacctcc ggcttgccag
      361 cttacaggcc tggctcagac gtggtcctgc agatggagat ctccttacga ggatcgcgaa
      421 agcagggcaa agcctgtatg atccctacac gggactaccg atgttggtga acatgaaaaa
      481 aggcgtcctt tacagtgtcg gccacgacgg gaaagatcag gatgccgatc cttcgtcgga
      541 tgtggtggta gagattcctg ccatacagat gtctgccgct caggccaaag catcagccgc
      601 ttcatccaag tccagataag catttcccca tactcccgcc tgccctgaca acgctcgctt
      661 gacagcgctt cggagtattt cacacaatca gccagccata tatcgatgaa gaggtctgtc
      721 atggaacatt caccaagacg attctttaac agtctctccg tgaggaaggg cgcgacgttt
      781 ccaactgctg ttccgacgca gatcgtatcc aacgaggaat tcctcccgat tcgtcaaacg
      841 gccgaacagg cttgcgtaga aaaggtaacg acagagttga tggaaaaggc ggcagctcgg
      901 cgtggattga cacgccgaga cttcttgaag accactggtg gtatggcggc tgcgctactg
      961 gccatgaacg aggcattcgg acgatttttc aatatcggag aaatcgaatt gtt
//
LOCUS       DASTWE010000999         1794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_349896, whole
            genome shotgun sequence.
ACCESSION   DASTWE010000999 DASTWE010000000
VERSION     DASTWE010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349896"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFT30_10425"
     CDS             complement(<1..945)
                     /locus_tag="VFT30_10425"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093093.1"
                     /translation="MLRVITGRFHPHLESALVDRLTSLKATDPLTPIAIIVPSKPLAD
                     RVRALLAVEHRLSLLNLHVLTFHQLALRLAEEVPPAYPLPRVVDELFFEQLVRHIVRD
                     KLSSLVALQRIGQSAGTWGALWATIRDLKDADVDPVQALQGLREGYFEEDDREWLGEL
                     ISLYAAVKEAGKTLRVGTQDDLAESLITFVPTASFLQPLSEVFYYGFYDLTQVQLSLF
                     EAISRAKETTLFFPLEDDPACGFAKRFFDRYIQPQVRSDKAMIRLEQESSFVATSPVQ
                     LTVRSVIGAEEELATTCRTILALVETHGYRFDEIGVTAR"
     gene            complement(962..1117)
                     /locus_tag="VFT30_10430"
     CDS             complement(962..1117)
                     /locus_tag="VFT30_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093094.1"
                     /translation="MDEDDIELPVLPKLDKGPPPDCPICGDPMSFIDGDWACVDCNGE
                     LLGPETG"
     gene            complement(1125..1391)
                     /locus_tag="VFT30_10435"
     CDS             complement(1125..1391)
                     /locus_tag="VFT30_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU5093095.1"
                     /translation="MPNITLLVSPSCGACPSAKSLWKQLRVKYSFSYREVDITTKDGQ
                     ELADRHSVRAVPATIIDGRLTFVGVPSRESAEKAIQLKIKQREG"
BASE COUNT          408 a          479 c          508 g          399 t
ORIGIN      
        1 acgggcggtg acgccgatct cgtcgaatcg ataaccgtgt gtttcgacca gagccagaat
       61 cgtgcggcag gtggtcgcca gttcttcttc tgccccgatc acactccgca cggtgagttg
      121 gacgggagag gtggccacga aggacgattc ctgttccaat cggatcatcg ccttgtctga
      181 cctcacctga ggctggatat atcgatcgaa aaaccgcttg gcaaatccgc aggctgggtc
      241 atcctccagc ggaaaaaaca gcgtcgtttc cttcgctcgg ctgatggctt caaagagaga
      301 cagctgcacc tgcgtcaggt cataaaaccc ataataaaac acttcgctca acggctggag
      361 gaacgaggcc gttggaacaa aggtgatgag cgactccgcc aggtcgtctt gcgttccaac
      421 cctgagcgtc ttgcccgctt ccttcaccgc cgcatagaga gaaataagtt cgccaagcca
      481 ctctctgtcg tcctcctcga aatagccttc acggaggcct tgcagcgctt gcactggatc
      541 gacatcggca tccttcaagt cgcgaatcgt cgcccagagt gccccccacg ttccagcaga
      601 ttgcccgatt cgttgtaatg caaccaggct ggagagtttg tcgcggacaa tgtggcggac
      661 caattgttcg aagaacagtt cgtcgacgac ccgcggcagc gggtaagcag gaggtacctc
      721 ttcagccagc cgcagggcca attgatggaa ggtcaatacg tgtaggttaa ggagtgacag
      781 tcggtgttcg acggcaagaa gggcgcgaac acgatcggct aacggcttgg acggaacgat
      841 gatcgcgatg ggcgtaagcg gatcggttgc cttgaggctc gtgagacggt cgacgagagc
      901 agattcaaga tgaggatgaa accgaccggt gatgactcgc aacatggcgg gtcattgtac
      961 gctacccggt ctccggtccc aagagctcac cattgcagtc gacacaggcc caatcgccgt
     1021 cgatgaagga catgggatcg ccgcagatcg gacaatcggg aggcggcccc ttatcgagtt
     1081 taggaagaac cggtagttcg atatcgtctt cgtccatggt ctccctatcc ttcccgttgt
     1141 ttgatcttca attgaatggc cttttccgcg ctctctcgac tcggcactcc gacaaacgtc
     1201 aaccgtccat cgatgatagt tgcagggacg gcacgaacgg aatgccgatc ggccagttcc
     1261 tgaccgtctt tcgtcgtaat atcaacttct cgataagaga agctgtattt aactctcagc
     1321 tgtttccaca ggctcttggc agacggacag gctccacagc tgggtgacac aagtaaagtg
     1381 atattcggca tcgtcagacc agtaaccggt taaggctgag gttcaggctg agataacgat
     1441 gagtgcggat gagtgtatgt ctctaaactc aacctcgacc tgaaccttaa tctttctcgg
     1501 ttagatctta gcaccggctg aaaagtcggt gcaaggagcg tctcgcccag tagcacaccg
     1561 acggattgga caatcgagag agaatttatc ttcctgcggg ccatccgccg gacgaatttt
     1621 ccgtgccatt aatgatgggg ggagagccag ctggattgcc agctgggttt cccggtgggg
     1681 ctacgatgtt gcccgcggta ttgggatagt aatggtaagg aggttcagtt ctaacgatgg
     1741 tgctccctgg atccccggta ttttgagcca gagaattttt agtcgccttc ggca
//
LOCUS       DASTWE010001000         1335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_414203, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001000 DASTWE010000000
VERSION     DASTWE010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414203"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFT30_10440"
     CDS             <1..181
                     /locus_tag="VFT30_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093096.1"
                     /translation="NFGLRAGLDGHLCGFLGVEHAEVYERFGSAAAMRTLSDGASRGV
                     TPKQDADTDLERICA"
     gene            complement(405..563)
                     /locus_tag="VFT30_10445"
     CDS             complement(405..563)
                     /locus_tag="VFT30_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093097.1"
                     /translation="MIEVIYRREVPYSRTVVLSQYFDLEHLEFVHREKWLKCLTKSLP
                     PLNRLRMW"
     gene            complement(611..1039)
                     /locus_tag="VFT30_10450"
     CDS             complement(611..1039)
                     /locus_tag="VFT30_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5093098.1"
                     /translation="MKRYSLHYNSQCTECARLAELNRRLDWLSRFERTTVPSTLGTPE
                     TTLGLKESRFFGNEKQGFVEYDIGPGTLAIGIGAPEWKPSRGGGSVALEVDDFSGAMN
                     RLRECGCTFTLEPMETPVCHMAAVLDPDGNSVMIHQRKTG"
BASE COUNT          324 a          367 c          353 g          291 t
ORIGIN      
        1 taacttcggg ttacgtgcag gattggatgg acacttatgc ggtttcctcg gcgtcgaaca
       61 cgccgaagtg tacgagcggt tcgggagcgc ggcagcgatg aggacgttgt ccgatggtgc
      121 ttccagaggg gttacgccca aacaagatgc agatacggat ctggaacgaa tttgcgcgta
      181 agttcggatg gaacgatgcc gcctccaaat ttctcactcg aatgaaacaa gaagatggtc
      241 tggagcatcg caccgatatc gtgaccactt ttgaactgat tgatctcaga gaaggcagag
      301 caggtcaacc accaagcgag acatagcaca gtgctgaatg gatgggacat gaatcagagt
      361 tgcctcgacg ctctccatgc actctacgaa cgcatgtctc acctctacca catgcgcaac
      421 cggttcaagg gagggagact cttcgtcagg cactttagcc atttctcgcg gtgaacaaac
      481 tccaagtgtt ccaggtcgaa gtattgcgac agcacgacgg tccgagaata gggcacctca
      541 cggcgataaa tgacttcaat catgtcaggc ttcctctcac ccgtgcaatt ggtgccaaga
      601 agcgacgtat tcatcccgtt tttctctgat gaatcatcac agagttccca tcgggatcta
      661 agactgccgc catgtgacac acgggagtct ccatcggctc aagcgtaaat gtgcagccgc
      721 actctcgaag acgattcatc gctccactga aatcgtcgac ttcgagagcc accgaccctc
      781 cgccacgtga aggcttccac tccggcgctc caataccgat cgcgagcgtt cctggcccaa
      841 tatcgtattc gacaaagcct tgcttttcgt tcccgaagaa ccgcgactcc ttaaggccca
      901 aggtcgtctc cggcgtacca agagtggagg gaaccgtcgt tcgctcaaaa cggctaagcc
      961 agtccaatct tcgattgagt tcagcaagtc ttgcacattc ggtgcattgg ctgttgtagt
     1021 gcagactgta tcgcttcatg ttaattcagg gcgtgggcgt tctcaacgaa gaccagcgat
     1081 ctgttgggtc gggcgcacca gcgactattc ctgcagatga gcgaggaaac gtgcgtggca
     1141 aatcatgagt tccccccctt ggctaacgtc acaaattcat tctgcttcga ttcaccgagc
     1201 ctcaaacaac ggctgcgagg caccggatcg aaaaggtctg agcaatcgtg aaagtcggcc
     1261 gaccaaccgt tgccatcgtg tcggcaggag cgagggttgt ggagaagaag tctcaccgcc
     1321 cgagtcactc atgat
//
LOCUS       DASTWE010001001         1365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_705505, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001001 DASTWE010000000
VERSION     DASTWE010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705505"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1197
                     /gene="tuf"
                     /locus_tag="VFT30_10455"
     CDS             10..1197
                     /gene="tuf"
                     /locus_tag="VFT30_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5093099.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVMSKHNPKMA
                     FRSFDSIDNAPEERARGITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLGRQVGIPTMVVFMNKADMVDDAELLELVEMEIRELLS
                     SYEYPGDDIPVIQGSALKALEGDAAWEPKIDELMQAVDDFIPTPARETDKPFLMPVED
                     IFTIQGRGTVATGRIERGQINVNEAVEIVGIKDTRSSVVTGVEMFKKLLDSGMAGDNV
                     GLLLRGVDRKEIERGQVIVKPGTITPHTKFKAEAYVLTKEEGGRHTPFFTGYRPQFYF
                     RTTDVTGVANLPAGVEMVMPGDNIQMEITLIAPIAMEKGLRFAIREGGRTVGAGIVAS
                     IIE"
BASE COUNT          332 a          359 c          401 g          273 t
ORIGIN      
        1 ggagaaccta tgagcaagga aaaattcgac cgcagtaagc cgcacgtgaa cattggaacg
       61 atcggtcacg tggaccacgg caagacgaca ttgacggcag ccatcaccaa ggtgatgtcg
      121 aagcacaatc cgaagatggc gtttcggtcg ttcgattcga tcgacaatgc accggaagag
      181 cgtgcacgtg ggatcaccat tgcgacggca cacgttgagt atgagacggc aaaccgtcac
      241 tacgcacacg tcgattgtcc gggccacgcc gattatgtca agaacatgat cacgggagcc
      301 gctcagatgg acggagctat cctcgtggtt gctgcaaccg atggtccgat gccgcagacg
      361 cgtgagcaca tcctgctcgg acgccaggtc gggatcccga cgatggtggt cttcatgaac
      421 aaagcggaca tggtggacga tgctgaattg ctcgagctgg tcgagatgga gatccgtgag
      481 ctgttgagct catacgaata ccccggcgac gacattcctg tcattcaggg ctcagccttg
      541 aaggcactgg aaggtgatgc cgcgtgggaa cctaagatcg acgagctgat gcaggcagtg
      601 gatgacttca ttccgacacc cgcccgcgag acggacaaac cgttcctgat gcctgtcgag
      661 gacatcttca cgatccaggg acgcggaacc gttgctacgg gacgtatcga gcgtggacag
      721 atcaacgtta acgaagcggt cgagatcgtc ggcatcaagg acacgaggag ctcggtcgtt
      781 accggtgtcg agatgttcaa gaagcttctt gattcgggaa tggcaggcga caacgtcgga
      841 ctgctgctcc gcggtgtcga ccgtaaagag atcgaacgcg gacaggtcat cgtaaagccg
      901 ggcacgatca cgccgcacac caagttcaag gccgaggcct atgtcctcac gaaggaagaa
      961 ggcggacgtc acaccccgtt ctttacaggc tatcgtccgc agttctactt cagaacaacg
     1021 gacgtgacgg gtgtcgcgaa cctgccggcc ggtgtcgaga tggtgatgcc tggagacaac
     1081 atccagatgg agatcaccct gatcgccccg atcgcaatgg aaaagggact tcgcttcgct
     1141 atccgcgaag gcggccgcac cgtcggtgca ggcatcgttg cttccatcat cgagtaatcg
     1201 gaattatccc cgattttagg aaaaaccccg ctggaaacgg cggggttttt tcattttccg
     1261 gcatgcggaa acgctcatga gatgttgtaa aaatatcgtt aagatgtccc ataacagtat
     1321 tttgttcatc tagcgttcag aaagcgtttt gcgttccggc atttt
//
LOCUS       DASTWE010001002         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1448867, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001002 DASTWE010000000
VERSION     DASTWE010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448867"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFT30_10460"
     CDS             complement(<1..418)
                     /locus_tag="VFT30_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5093100.1"
                     /translation="MPPIMLLSCESLGKSYGVKPLFTDLSLGLCEGDHVGLIGPNGSG
                     KSTLLKILAGLENPDCGTRSVRRQLRIGYVPQEPLFAEQHTVEDALSQVLLDEGLDPH
                     EQGARLAKALSLGGFVRSDQSVSTLSGGWKKRLAIAR"
     gene            complement(505..1266)
                     /locus_tag="VFT30_10465"
     CDS             complement(505..1266)
                     /locus_tag="VFT30_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5093101.1"
                     /translation="MPKRVRTGLTRSDILDRYVERFKQQLVKFQPFLSRKRGSVSLED
                     FDEAAEELISQVFGGASDESEAYFFAKTGESALLPEEAQESGTHNVERESLQQRRQVL
                     ESCLADLELRRRLQATRQGKGIGQALVSDYMSHDVRSIHRDASIKQAGQLLQKHKVGS
                     LIIDDGSRYIGIVTDSDLTRKAVAKGLDPNTTTVATCMSRPLVTIEEDESLTEAMLLM
                     KKQSIRHLPVTADATIIGVLSVSDLLRAFEEQKTP"
     gene            1450..>1709
                     /locus_tag="VFT30_10470"
     CDS             1450..>1709
                     /locus_tag="VFT30_10470"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEU5093102.1"
                     /translation="MKELLFIALLILLSSGPVYAGWEVLEKQYQPTGLETLYFDPETI
                     HREGTRAMLWQLTDLKWNSTTRFLSFKTHKEFDCEQSRVRVL"
BASE COUNT          354 a          480 c          454 g          421 t
ORIGIN      
        1 atcgtgcgat cgccaaccgc ttcttccatc cgcccgacag cgtcgagacg gattggtctg
       61 agcgaacaaa cccgccgagg ctgagggctt tggcaagacg tgctccttgc tcatgtgggt
      121 ctaatccctc atccagaagg acttgggaca gcgcatcttc gaccgtgtgt tgctcagcaa
      181 acaagggttc ttgcgggaca taaccgatac ggagctgccg ccgcaccgat ctggtgccgc
      241 aatccggatt ctccagaccg gccaggatct tgagcagcgt cgatttgccc gatccgttgg
      301 gaccgatgag tccgacgtga tccccttcgc agagtccaag cgacaggtcg gtaaataacg
      361 gcttcactcc atagcttttt ccaagagact cgcaactgag cagcatgatg ggtggcatga
      421 cagcagtgta cctgatcaga gctctagcga gcaggcttct cggcaaccgc ttcgtgcagc
      481 tgctagcagt tagctgtacg aaggctacgg agttttttgt tcttcgaacg cgcgaaggag
      541 gtcagagact gaaagtaccc cgatgatcgt ggcatcggca gtgaccggca ggtgtcgaat
      601 gctctgcttc ttcatcaaca acatggcttc ggtgagcgat tcatcttctt caatcgtaac
      661 aagtggccgg ctcatgcaag tggcgacggt cgtggtattc gggtcaagcc ctttggcgac
      721 ggctttgcgt gtcaggtctg aatcggtgac gatgccgata taccgcgacc cgtcgtcgat
      781 gatcagcgaa ccgactttgt gcttttgcaa cagttgccca gcttgtttga tgctcgcgtc
      841 ccgatggatg cttcgcacgt catgtgacat gtagtccgaa acgagtgcct gtcctatccc
      901 tttgccctgt ctggttgcct gcaatctacg gcgtaattcc aggtcagcca ggcagctttc
      961 cagcacttgg cgtcgctgtt gaagactttc acgttccaca ttgtgggttc cgctttcctg
     1021 cgcttcttca ggcaaaagtg ccgattcccc tgtctttgcg aagaagtatg cctcagattc
     1081 atccgatgcc ccgccgaaca cctggctgat cagctcttcg gcggcttcat cgaagtcttc
     1141 cagtgaaacc gacccgcgtt tgcgcgagag gaagggttga aatttcacga gctgttgctt
     1201 gaaccgctcg acataccgat cgagaatatc actccgcgtc aaccctgtgc gaactcgttt
     1261 cggcatcgtc ttcctcctgg ttgtgagcgg gagaataacc tatgtgaagt tgacgctcaa
     1321 gaggtccctg aggcacatcc ttcaacatca tcttatctga accttgtcgc ttgatttttt
     1381 atctgcgacc ccttatgcaa tccgactcga cctcgttgct tcgatcggtg ccgaacggga
     1441 tgacctgcca tgaaggaatt gttgtttatc gccctgctga tattgctcag ttccggcccg
     1501 gtctatgctg gatgggaggt cctcgagaaa cagtaccagc ctaccggtct cgagaccctg
     1561 tacttcgatc cggagacgat tcaccgagaa ggaactcgtg caatgctctg gcaattaacg
     1621 gatctcaagt ggaacagcac aacacgattc ttgtccttca agacccacaa ggaatttgac
     1681 tgtgagcagt cgcgcgtacg cgtcctgca
//
LOCUS       DASTWE010001003         1381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1286834, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001003 DASTWE010000000
VERSION     DASTWE010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286834"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFT30_10475"
     CDS             <1..851
                     /locus_tag="VFT30_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5093103.1"
                     /translation="LRLLDKQNLMLDMTKLGFEPESLTKFKRNISKPYGMVLVTGPTG
                     SGKTNTLYSALQSLNTVETNIMTAEDPVEFNLPGINQVQMKEQIGLNFAAALRSFLRQ
                     DPNIVLVGEIRDFETAEIAIKAALTGHLVLSTLHTNDAPSTISRLMNMGIEPFLVATS
                     VNLIQAQRLIRRVCKDCKQEHQTPVEALIEVGFSTEEAKTIKTYKGKGCSTCNNTGYK
                     GRIGLYEVMEITDELRELILIGASALELRKKAVDDGMITLRESGLYKIRQGVTTPEEV
                     VRETVA"
     gene            867..>1381
                     /locus_tag="VFT30_10480"
     CDS             867..>1381
                     /locus_tag="VFT30_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093104.1"
                     /translation="MLRPIAIALILVLSVGIMLPFANSAHGLRQTVQVGQRRHHRYRS
                     RAWWRRYRARQRQKRLAAEMAHRNATLSLPQNLSTADLQGLSGPQLPSLPSNSAAPTT
                     LPPPEMTAAPVAAVSNAAHASSLTSGETRPRVVNPNHPAFKTSAPATPLPGTMNLAVI
                     AMSRPNPAFLTS"
BASE COUNT          339 a          403 c          355 g          284 t
ORIGIN      
        1 tgcttcgttt gctcgacaag cagaacctga tgcttgacat gacgaagctc ggcttcgagc
       61 cggagtcgtt aacgaaattc aagcgaaaca tctccaagcc ctacggcatg gtgctggtaa
      121 ctggtccaac cggaagcggt aagacgaaca cgctttactc cgcgctccaa tcgttgaaca
      181 cagtcgaaac caacatcatg acggcggaag acccggtaga gttcaacctt cccggcatca
      241 accaggtgca aatgaaagag cagattgggt tgaacttcgc cgcagctctt cgatcattcc
      301 tacgccagga tcccaacatt gttctggtcg gcgaaatccg cgacttcgag accgctgaaa
      361 tagccatcaa agccgcgctg actggtcacc tcgtactctc taccctccac actaacgatg
      421 ctccttccac catctcacgt ctcatgaaca tgggcatcga acccttcctg gtcgccacca
      481 gtgtgaacct catccaggca caacgactga ttcgtcgcgt ttgcaaggat tgcaagcagg
      541 aacaccaaac gcccgttgaa gcactaatcg aagtgggttt ctcaaccgag gaagccaaga
      601 cgatcaagac ttacaagggc aaaggatgtt cgacctgcaa caacaccggc tacaagggac
      661 gaatcggact ctacgaagtg atggaaatca ccgacgagct tcgtgaattg atcttgatcg
      721 gagcttctgc acttgaatta aggaagaagg cggtcgacga cggcatgatc acgttacgcg
      781 aatccggtct gtacaagatt cgccagggcg tgaccacccc cgaggaagtc gttcgtgaaa
      841 cggtcgccta atcgaggaga acgaagatgc taagaccaat tgcaatagcg ctgatccttg
      901 tgctgtccgt gggcatcatg ttgcctttcg cgaactcagc acatgggctc aggcagactg
      961 tacaagttgg acagcgccgt catcaccggt atcgttcacg cgcgtggtgg cgacgttatc
     1021 gcgcgcgcca gcgacagaag agattggctg cggagatggc ccatcgcaat gcaactctgt
     1081 cgttgccgca aaacctttcg actgcggatc tacagggact ctccggtccg caattgcctt
     1141 ccctgccgtc aaactctgct gcgccaacaa cactgccgcc gcccgagatg accgcagcgc
     1201 ctgttgcggc tgtttcgaat gcggcgcatg cttcgtcgct tacaagtggc gaaacacgtc
     1261 cgcgagttgt taatccgaat catcctgcat tcaaaacgtc ggccccggcg acaccattgc
     1321 cgggaacgat gaatctcgcg gtgattgcga tgagtcgacc gaaccctgcg tttctgactt
     1381 c
//
LOCUS       DASTWE010001004         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1028792, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001004 DASTWE010000000
VERSION     DASTWE010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028792"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..>1060
                     /locus_tag="VFT30_10485"
     CDS             338..>1060
                     /locus_tag="VFT30_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5093105.1"
                     /translation="MKEATTVSNYDIKDLNQAEGGHRRIGWAELEMPVLRQIRERFKK
                     EKPLKGLRVSACLHVTAETANLMRTLQEGGADVVLTASNPLSTQDDVAAALVNRYEIP
                     TFAIKGEDNVTYYKHIRAALEHKPQMTMDDGADLVSSLFFIEMGRFEKLEPSLASWAK
                     ALPDEERKEFLAGVIGGTEETTTGVIRLKAMEADKVLKFPVIAVNDAQTKHFFDNRYG
                     TGQSTIDGIIRATNVLLAGKNFV"
BASE COUNT          262 a          274 c          298 g          226 t
ORIGIN      
        1 cacacggctt cgactggaag ttgtgcgggg agatcggttc gctggcggcg gtgtactgcc
       61 tcgagcagcg tggtccgcaa agtcataatt actcgcgcga agaattcatc cgtcgcttcc
      121 gccggcattt tgacgaccgc ggaaagctgg atgagctgtt gcgctagttg aatttggagt
      181 ttacgtttga ttggtttcat aaaaagtata atcaatcaca acgataatgt tagccagaga
      241 gtcacggagt caaagtctcc agactttgaa acttcaacga caggagtcgt tgactcccag
      301 cttctgtggc acagaattta tcacgaacgg agtaacaatg aaagaagcaa caacagtcag
      361 taactacgat atcaaagatc ttaatcaggc tgaaggtgga catcgccgca ttggttgggc
      421 tgaactggag atgcccgtgc tgcgccagat ccgcgagcgc ttcaagaaag agaagccgct
      481 caagggattg cgggtatcgg cttgtctgca tgtgacagcg gaaaccgcca acctgatgcg
      541 caccctgcag gaaggtggtg cagacgttgt gttgactgcc tccaatcctc tttcgacgca
      601 ggatgatgta gctgccgccc tggtgaaccg ctacgaaatt ccgacgttcg ccattaaggg
      661 ggaggataac gtcacctact acaagcacat tcgcgccgcc ctcgaacaca agccgcaaat
      721 gaccatggac gatggcgccg acctggtctc atccctgttc ttcatcgaga tgggccgctt
      781 cgagaagctg gagccttcgc tggcttcctg ggcaaaggca ttgcccgatg aagagcgcaa
      841 ggaattcctg gctggtgtca ttggcggcac agaagagaca accaccgggg tgatccgctt
      901 gaaggcgatg gaggcggaca aggtcctcaa gttcccggtc atcgccgtca atgacgcgca
      961 gaccaaacac ttcttcgata atcgctatgg gacgggccaa tcgacgatcg atggaatcat
     1021 tcgcgcgacc aacgtcctcc tggccggaaa gaattttgtc
//
LOCUS       DASTWE010001005         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_156125, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001005 DASTWE010000000
VERSION     DASTWE010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156125"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /gene="hisI"
                     /locus_tag="VFT30_10490"
     CDS             complement(<1..439)
                     /gene="hisI"
                     /locus_tag="VFT30_10490"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEU5093106.1"
                     /translation="MPDQFKFDGEGLLPAVIQDWLDGTVLMLGYMNQEALAKTVATKR
                     VHFWSRSRHKLWEKGETSGHTLCVKELFIDCDCDTILVKAQPAGPTCHTGEQACFFSR
                     IDEQGHVVHRNAQDAQGGILESVLRTIRERRSSPQTGSYTSKLF"
     gene            complement(459..1256)
                     /gene="hisF"
                     /locus_tag="VFT30_10495"
     CDS             complement(459..1256)
                     /gene="hisF"
                     /locus_tag="VFT30_10495"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEU5093107.1"
                     /translation="MSRSIEMLTKRIIPCLDVKEGRVVKGVSFVSLRDAGDPVEAAVG
                     YDHEGADELCFLDITASHENRKTIIDVVERTAARVFMPVTVGGGVGTLDDIRALLNAG
                     ADKVSINTAAVRRPEFVKEAAQRFGTQCVVVAIDAKRAEDDRWEIFTHGGRHATGLDA
                     VEWAKRMEQYGAGEILLTSMNQDGRQTGYDLDLTAAVSGAVSIPVIASGGVGTLDHLY
                     DGFVKGKADAVLAASIFHFRTYTILQAKAYLRERGVPVRLDNLSRVA"
     gene            complement(1240..>1758)
                     /locus_tag="VFT30_10500"
     CDS             complement(1240..>1758)
                     /locus_tag="VFT30_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU5093108.1"
                     /translation="KAVRVKVQIGGGIRTIDTVRRYLNAGVSRVVLGTAALTDRGFLE
                     QACQEFPQRIVLGLDARDGRIAVRGWTAVSDVKAIDLLKELACCAIAAVVYTDIARDG
                     MLNGPNLPALKEIVEFSSFPVIASGGITHLEDLRAIRSLGPRIEGAIVGKALYDGKLD
                     LQEAVAAFEQIH"
BASE COUNT          382 a          555 c          427 g          394 t
ORIGIN      
        1 cgaacagctt tgaggtatag gaaccggtct gaggactgga gcgacgctcc cgaatcgtcc
       61 gcaggacact ttcaagaatt cccccctgcg cgtcttgcgc attccgatga acgacgtggc
      121 cttgttcatc tattctagaa aagaagcagg cctgctcgcc cgtatgacaa gtcggcccag
      181 ctggttgcgc cttcaccaaa atagtgtcgc aatcgcagtc aataaagagt tcctttacac
      241 atagcgtgtg accggatgtt tcgccttttt cccagagctt gtgtcgagac cggctccaaa
      301 agtgcaccct cttggtcgcg accgtcttcg cgagggcctc ttggttcatg tatccgagca
      361 tcagcaccgt cccatcgagc caatcctgaa tgacggccgg gaggagcccc tcgccatcga
      421 acttgaactg atctggcatt ccctgctgac tcatcgtgtc atgccactcg agaaagatta
      481 tccaatcgga caggaacacc tcgttccctc aaatacgctt tcgcttgcaa gatcgtataa
      541 gtccgaaaat gaaaaatcga agcggccaag actgcgtccg ctttcccttt cacgaagcca
      601 tcgtataggt gatcgagcgt cccgactccg cctgatgcga tcacgggaat cgacacggcg
      661 cctgacaccg cagctgtcaa atccaagtcg tacccggttt gccggccatc ctgattcatg
      721 ctggtcaata agatttctcc ggcaccgtac tgttccatcc gctttgccca ctcgaccgca
      781 tccaatcccg tcgcatggcg accgccgtgt gtgaagatct cccagcgatc gtcctccgcg
      841 cgcttggcat cgatggcaac gacgacgcat tgtgtgccga atcgctgtgc ggcctctttc
      901 acaaactcgg gccgtcgtac tgccgcggta ttgatgctga ccttatccgc tccggcattc
      961 aacaaggccc gaatgtcgtc gagcgtcccc acaccgccac cgactgtcac cggcataaat
     1021 acccgggcag ccgtccgctc aacgacgtcg ataatcgtct tccggttttc atgcgaggcc
     1081 gtgatatcga gaaaacagag ttcgtccgct ccttcgtgat cataccccac tgcggcttca
     1141 acggggtctc cggcatcgcg gagactcaca aagctgacgc ccttgaccac gcggccttct
     1201 ttgacgtcca gacagggaat gatgcgcttg gtcaacatct caatggatct gctcaaaggc
     1261 agccaccgcc tcctgcaaat caagcttgcc gtcatacagt gctttgccga caatggcgcc
     1321 ctcgattctc ggaccaagcg atctgatggc tcgcaagtct tccaagtgtg tgattcctcc
     1381 cgacgcaatc accggaaacg acgagaattc gacgatttct ttcaaggcgg gaagattcgg
     1441 tccattcagc atgccatcgc gtgcgatgtc tgtatacacg acggcggcaa tcgcacaaca
     1501 tgcaagttcc ttcaataaat cgatcgcttt cacatcggat accgctgtcc agcctcttac
     1561 cgctattcgg ccatcacgtg cgtcgagccc cagtacgatc cgctgcggaa attcttggca
     1621 tgcctgctcc agaaaacctc gatcggtgag tgccgctgta ccgagcacaa cacgtgacac
     1681 tccggcgttg agataccggc gcaccgtatc aatggtcctg atgccgccac cgatttgaac
     1741 ttttacgcga accgcttt
//
LOCUS       DASTWE010001006         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_640752, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001006 DASTWE010000000
VERSION     DASTWE010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640752"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..415)
                     /locus_tag="VFT30_10505"
     CDS             complement(92..415)
                     /locus_tag="VFT30_10505"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5093109.1"
                     /translation="MGFKRKHSRVEVGSVGRLQRGSLSAPCKVLDVSESGVRLESRLF
                     VKIGDVLQLGIELEGGRSLTCEVQVIHVRAPKLGAKIISISPEDRERLSHILDDHVQS
                     SFSRG"
     gene            complement(481..843)
                     /locus_tag="VFT30_10510"
     CDS             complement(481..843)
                     /locus_tag="VFT30_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEU5093110.1"
                     /translation="MNIRTLSPCPSSPNCVSTQATDESHAIAPFQYNKSRAEAKEALK
                     AVLATLPRTKLVDEDESYLHYEFTSLLLRFVDDVEFLFDETTKTVHFRSASRTGYSDL
                     GVNRKRMEQVRTLAGGKL"
     gene            complement(840..1247)
                     /locus_tag="VFT30_10515"
     CDS             complement(840..1247)
                     /locus_tag="VFT30_10515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU5093111.1"
                     /translation="MKRLSGLKLLDEREGEGQPAQRGDHVIYNCRIFLNRGDEVLLNA
                     RQAEHLPKDMIRVVEGETFVDHKTTLGSRQTIAGVEQALIGMKAGGYRRVRVSPHLAY
                     RDKGVPDFIPANAVLVVELWLREIVQGRKVLLS"
     gene            complement(1255..>1901)
                     /locus_tag="VFT30_10520"
     CDS             complement(1255..>1901)
                     /locus_tag="VFT30_10520"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEU5093112.1"
                     /translation="FTFFLPVMTGYMNAVIFVIGAIASVAVILRVAQLIYRNNPDRSR
                     REAIGVTVPAAALIGMLVGFYFLNWIPPVPLSLKFGGIYHDVKRAGDQFELAFEKRWY
                     EFWKRSDTTYPANQPIYCSTAVFAPVDLNTTIYHHWYFRPNSEKPFVHADRIPLKISG
                     GREGGYRAYSFKQRLDPGDWRIDVESQDGRIIGRVSVKVVDQGEVQPSLTTVTY"
BASE COUNT          453 a          547 c          480 g          421 t
ORIGIN      
        1 cacctggaag aatggcctag cctatctccc caactccact tttcaaaaga ggaactgcag
       61 ggcgagtgcg gtctacggtg cgtcgacgcg cctaaccgcg ggaaaaacta ctctggacat
      121 ggtcatcgag aatatgactc aatcgctctc gatcttcagg actgatcgaa atgatttttg
      181 cgcctaattt aggcgcacgc acgtgaatga cttgcacctc gcaggttaag gatctcccac
      241 cctcaagctc tatcccaagt tgaagcacat caccgatctt cacgaacaga cggctttcta
      301 gacggacgcc ggactcgctg acgtcgagca ccttacacgg cgccgagagc gaaccgcgct
      361 gcaaccggcc gacagagccg acctccacac gtgaatgctt acgcttgaac cccataccga
      421 tcctcctatt ccggcatcat aacaggagga tggatcatgc cgaagcaatt acagaaatgc
      481 ctaaagcttt ccgcccgcta aggttcgcac ctgttccatg cgtttgcggt tgacgccaag
      541 atcactgtag cccgtgcggg aagcagagcg aaagtgaact gttttcgtag tttcatcgaa
      601 gagaaactcc acatcatcga cgaagcggag cagcaggctg gtgaactcgt agtggagata
      661 ggactcgtcc tcgtcgacga gcttggtccg aggcaacgtt gcgagtaccg ctttcaaggc
      721 ctcttttgct tctgcccgag acttgttgta ctggaatggc gcgatggcgt ggctctcatc
      781 agtcgcctga gtcgagacgc agttggggct ggagggacag ggagaaagag tccggatatt
      841 catgatagga ggactttacg cccttgaacg atctcccgca accagagctc cacgaccaag
      901 accgcattgg caggaataaa atcaggaaca cccttgtccc gataagccag gtgcgggcta
      961 acgcggactc tccgataacc gccggctttc ataccaatca gcgcctgctc gacgccggct
     1021 atagtttgac ggctgccgag tgtcgtcttg tgatcgacga atgtctctcc ttccacaact
     1081 cgtatcatgt ccttaggaag atgctcagcc tgtctcgcgt tcagtagcac ctcatcaccc
     1141 ctgttcaaga agattctaca gttgtagatg acgtggtcgc ccctctgcgc cggttgtccc
     1201 tcgccctctc gctcatctag gagcttgagt ccgctcagcc gtttcatcct gtgcttaata
     1261 tgtcaccgtc gttagcgacg gctgcacttc tccttggtcg accactttca ctgagactcg
     1321 cccaataata cgcccgtctt gagattcgac atcgatccgc caatcgcctg gatcaaggcg
     1381 ctgtttgaaa ctataggctc gatagccacc ctcgcgccct cctgaaatct tgaggggaat
     1441 tctatccgca tgcacgaacg gcttctcact gttcggacgg aagtaccaat ggtggtaaat
     1501 cgtcgtgttg agatcgaccg gtgcaaagac ggcagtggag caatagatcg gctggttcgc
     1561 cggataggtc gtatctgagc gtttccagaa ctcataccac ctcttctcaa aagccagttc
     1621 gaactggtct ccggctcgct ttacgtcatg atagatccca ccgaatttga gtgacagcgg
     1681 cacggggggg atccaattca ggaaataaaa cccgaccagc ataccgatca atgcagcggc
     1741 aggaacggtc acaccgattg cttcacgtcg cgatcggtcc ggattattac gatagatcaa
     1801 ttgcgccact cgcaagatga ctgctacgct cgcgatggct ccgatcacaa aaatcacagc
     1861 attcatgtag ccggtcatca cgggcaaaaa aaacgtgaaa a
//
LOCUS       DASTWE010001007         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1286970, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001007 DASTWE010000000
VERSION     DASTWE010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286970"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFT30_10525"
     CDS             complement(<1..441)
                     /locus_tag="VFT30_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5093113.1"
                     /translation="MKSLSGKVAIVTGASNGIGRAIAERLADNGAIVVVNYSKSVEKA
                     QQVVAGIQGKGGKALAVQADMSQVADARRLVIDTFKQFNRLDILVNNAGTFMPKPLEE
                     TTEEDFNSVIALNAKGPYFAMQEAARALKDGGRIVNISTGVTHLH"
     gene            complement(475..>1080)
                     /locus_tag="VFT30_10530"
     CDS             complement(475..>1080)
                     /locus_tag="VFT30_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5093114.1"
                     /translation="VRTMEKPAQTQFPIHDLLVRRWSPRAFDERPVESHTLWSLFEAA
                     RWAPSSNNEQPWRFIAATKRDHEADWTRLFDCLVEGNRRWAFRAPVLMLSIASINFED
                     DGKPNRHAFHDTGLAVGNVILQATAHGLIVHQMAGFDVEKARSYLSIPSGCEPVAMIA
                     IGYPGDPAVLPERLRERELRPRSRRPIREWAFLGQWGIPIP"
BASE COUNT          218 a          326 c          280 g          256 t
ORIGIN      
        1 gtggagatgc gtcacaccgg tcgagatatt cacgattcgt cctccgtctt tcagcgctct
       61 tgccgcttcc tgcatggcaa agtatggtcc cttggcattc agggcgatca cgctgttaaa
      121 gtcttcttcg gtcgtctctt caagcggctt cggcatgaat gtcccggcat tgttcacgag
      181 gatatccagc cggttgaatt gtttgaacgt atcgatcacg agtcggcggg cgtccgccac
      241 ctgactcata tcggcttgaa ccgctaacgc cttgccgcct ttgccttgga tccccgcaac
      301 gacctgctga gctttttcca cactcttgga ataatttact acgacgatgg cgccattgtc
      361 cgccaggcgt tctgcaatcg ctcgaccgat tccgttggag gccccggtca caatcgccac
      421 tttcccgctc aacgatttca tcgattagac tcctcttttc gagccatgac agtgctaggg
      481 aatgggtatt ccccactgtc ccaaaaacgc ccattctcga ataggccggc gacttcgagg
      541 ccgcaattcg cgttctcgca gccgctctgg aagaacggcg ggatcacctg gatagccgat
      601 ggcgatcatc gcgaccggtt cgcagcccga aggaatactg agatagctcc gcgccttctc
      661 cacgtcgaaa cccgccatct gatggacaat caacccatgt gcggtcgcct gtaagatgac
      721 gttcccaacg gctaatcctg tatcgtgaaa ggcatgccgg ttcggctttc cgtcatcttc
      781 aaaattgatg ctcgcaatcg agagcatcag gaccggcgcg cggaaagccc atcttcggtt
      841 cccctcgacg agacagtcga acagtcgagt ccaatccgct tcgtggtctc ttttggtcgc
      901 ggcgataaat cgccaaggct gttcgttgtt tgaggaaggc gcccagcgag ccgcctcgaa
      961 cagagaccac agcgtgtggg attcgaccgg tctttcatcg aacgcccgag gactccaacg
     1021 gcgaacaagt aagtcatgga tcggaaattg ggtctgcgcc ggtttttcca tggtcctcac
//
LOCUS       DASTWE010001008         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1319438, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001008 DASTWE010000000
VERSION     DASTWE010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319438"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1631)
                     /gene="mgtE"
                     /locus_tag="VFT30_10535"
     CDS             complement(174..1631)
                     /gene="mgtE"
                     /locus_tag="VFT30_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU5093115.1"
                     /translation="MQTERPIEPRVPDQRPRQSERDMLREVLRDQTERGQTKSDIVIQ
                     SVQKLLRRGAITNLSKMLGRMHPADIAKVVTHLSSPKEKREIFELVRGEGKRGQALSE
                     LDGESIQQVLADLLHSDIAWLLKDLGPDDVAYILGFLPEERSKEVLALMKTEDSTEVA
                     DILKYPKDTAGAIMTTEFFSLPEDATAQEAIRRLQQATDAEMVFYIYVTDKDDRLVGV
                     LSLRQLITVPPTTPLKNIMTREVMSVAIDMDQEEVARQVASYNLLAIPVVERDGKLVG
                     IITVDDVVDVIREEATEDMLKMAGAIEEETGLKSSSFGSAKLRLPWLFTNLVGSLLSG
                     AILWYFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIRGLATGSVELTHVWPIFFR
                     EAKIGLVMGVACGVTLTLVAWVLHQGFLGMVVGTSLIIAFLVSTSMATIMPILLKRVG
                     VDPAVAAGPFVTTANDITGITIYLTLATFFLEYLR"
     gene            complement(1642..2526)
                     /gene="era"
                     /locus_tag="VFT30_10540"
     CDS             complement(1642..2526)
                     /gene="era"
                     /locus_tag="VFT30_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU5093116.1"
                     /translation="MKFGTVAIIGRSNVGKSTLLNRLLGEKISIVSSKPQTTRTRIMG
                     VVHVEGAQIAFLDTPGLHKPEHLLNRRMVRTAVETMEDADLFYMLMDATSLPGPGDLT
                     AVKHMKEALAKQPRPVILVVTKIDLVNKQKLLPTLDHYGKLFAWTEIVPVSAQADDNV
                     DRLLAVTVPYLPSGEGAYGDDVVTDQTMRTLAAEMIREKVLQETEEEVPHAVAVEIDE
                     FVEHGKLAKIRASILVERETQKGILIGKQGERLKAIGTQARLDMERLFGMKVFLELWV
                     KVKKAWREDEQTLVQLGY"
     gene            complement(2523..>2863)
                     /locus_tag="VFT30_10545"
     CDS             complement(2523..>2863)
                     /locus_tag="VFT30_10545"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5093117.1"
                     /translation="GRQWLEWRQRVLLVVQVIGVGGCGWLIVALFTGEMGVTRYLSMR
                     DHAKNLEQELSTLRRDNATLQQDIIRLQHDPAKIEQLAREQLGYVRKGETVYQLVPDP
                     EKKTEPISKP"
BASE COUNT          645 a          853 c          691 g          674 t
ORIGIN      
        1 gggcaccacg agctacacct cggattttac ccagttcctt ggaatataag gtgatgattc
       61 gatctgcttc gccccattta cggctccgta agatgatcgc cgtcgtcttt accagaggca
      121 cggctctctc cactccctac cgcgacagaa gtatgggaac gctccagtcc gcgtcaccgg
      181 agatactcta aaaaaaaagt ggctaaagtc aggtagatgg taataccggt gatgtcatta
      241 gctgtcgtga caaaaggacc ggccgcaacg gccggatcga cccccacgcg tttgaggaga
      301 atcggcataa tcgtggccat gctggtcgac accaaaaatg caatgatcag cgaagttcct
      361 acgaccatgc ccaaaaaccc ttgatgcaaa acccaggcaa ccagtgtcag tgtcaccccg
      421 caggccactc ccatgacaag accaatcttc gcttcacgaa aaaagatagg ccatacatga
      481 gtgagttcca cagagccggt ggccaatcct cgaataatca gtgtcgagga ttgcaacccc
      541 acattaccgc ccatggcagc aatcacgggg ataaaactca caatcgccac cacttcttgg
      601 attgtataac ggaaatacca aaggatcgca ccggacaaga gactgccgac caaattggtg
      661 aagagccacg gcaaccgcaa tttcgccgag ccgaagctcg aagacttcaa gcccgtttct
      721 tcttcaatcg cccctgccat tttcaacatg tcttcggtcg cttcttcccg gatcacatcg
      781 actacgtcat caaccgtgat gatgcccacg agtttgccgt cccgttccac aaccggaata
      841 gccagcaagt tatagctggc cacctggcgt gcgacttctt cttgatccat gtcgatcgcc
      901 acactcatga cctcgcgggt catgatattt tttagtggag tcgtcggagg gacggtgatc
      961 agctgccgaa gcgagaggac accaaccagt cgatcatctt tgtccgtcac ataaatatag
     1021 aacaccattt cggcatccgt ggcttgctgt aaccgacgga tcgcttcctg tgccgtggca
     1081 tcctccggca aggaaaagaa ctccgtggtc ataatcgcgc ctgccgtatc cttcggatac
     1141 tttagtatgt cggccacctc ggtcgaatcc tcggtcttca tcaaggcgag aacttctttg
     1201 ctacgctctt cgggaaggaa tccgagaatg tacgccacgt cgtcgggacc aaggtctttc
     1261 aaaagccacg cgatgtcgga atgcaacaga tccgcgagta cttgctggat actttcgccg
     1321 tcgagttcac tgagcgcttg cccacgcttg ccttcgccac ggaccaactc gaaaatttca
     1381 cgtttttcct tcggagagga gagatgggtt acgactttgg cgatgtccgc gggatgcata
     1441 cgacccaaca tcttggagag gttggtaatg gctccgcgtc gcaacaattt ctgcaccgac
     1501 tggatcacaa tgtccgattt cgtctgcccc cgttcggtct gatcacgcaa gacttcccgc
     1561 aacatgtcgc gttcggactg gcgaggtcgc tgatccggta cccgcggttc tatcggtcgt
     1621 tccgtctgca tgcctgtttc gtcagtaccc caactgcacc aaggtctgct cgtcctcgcg
     1681 ccacgctttc ttcaccttca cccacagttc aaggaacact ttcatgccga ataaccgttc
     1741 catatctagt ctggcttgcg tgccgatcgc tttcaaacgc tctccttgtt tgcctatcag
     1801 gatgcccttc tgcgtctctc gctccaccaa aatcgacgcc cggattttcg ccaatttgcc
     1861 atgctcgacg aactcatcga tttcgaccgc aactgcatgc ggcacttctt cctccgtctc
     1921 ttgcaggacc ttttcacgga tcatctcagc tgccagcgtc ctcattgtct gatccgtcac
     1981 gacatcgtcg ccgtaggctc cttctccaga cggaagatag gggaccgtca cggccaacaa
     2041 ccggtcaaca ttgtcgtcgg cctgggcaga aaccggcacg atctcggtcc acgcgaagag
     2101 tttgccataa tggtcgagag tcgggagtag tttctgcttg ttgacgagat cgattttcgt
     2161 gacgacgagg atcaccggac gcggctgttt ggccaacgcc tccttcatat gcttaacagc
     2221 ggtcagatct ccgggacccg gcagactcgt cgcatccatg agcatgtaga acagatccgc
     2281 atcttccatg gtttccacgg ccgtacgcac cattcgacga ttcagcaagt gctcgggttt
     2341 atgaagcccc ggcgtgtcga gaaaggcaat ttgtgctcct tccacatgca cgacgcccat
     2401 aatgcgggtc cgtgtggttt gaggcttact cgacacaatc gaaatttttt cgccaagcag
     2461 acgattgagg agtgtcgact tgccgacgtt ggaccggcca atgatggcta ctgttccgaa
     2521 cttcatggtt tcgagatggg ctccgttttt ttctcaggat ccggcaccag ttgatacaca
     2581 gtttcacctt tgcgcacata gcccaactgc tctcgagcca actgttcgat tttggcgggg
     2641 tcatgctgga gacgtataat atcttgctgc aatgtggcat tgtctcgacg taaagtcgag
     2701 agttcttgct ccaaattctt ggcgtgatca cgcatggaga gatacctcgt gacgcccatt
     2761 tctccggtaa acaaggccac gattagccac ccgcatccac cgacaccgat cacctgcacg
     2821 acgagaagta cgcgctgccg ccattccagc cactgccgtc cac
//
LOCUS       DASTWE010001009         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_544105, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001009 DASTWE010000000
VERSION     DASTWE010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544105"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFT30_10550"
     CDS             <1..320
                     /locus_tag="VFT30_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5093118.1"
                     /translation="SLAQRAALAALGDDEHVAKSRAVNTAGMEQMEQGLRALGVIPIP
                     SETNFLYFDAKRDGRLVFEALLRAGIIVRHIDGTMLRVTIGQPDENAAFLQAFRKVLS
                     GDR"
     gene            338..>1068
                     /locus_tag="VFT30_10555"
     CDS             338..>1068
                     /locus_tag="VFT30_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5093119.1"
                     /translation="MIIVLKPEASESEVDHIIDRLRDLGLKSQISTGQERTIIGVIGD
                     DRILHNQPLTALPGVESVLPILAPWKLVSREFKKEATIIDVGGVKIGAKKLAIMAGPC
                     AVERLELTVGIAHEVKASGASILRGGAYKPRTSPYSFQGLGREGLDYLVEARKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNQNVMLCERGIRTFET"
BASE COUNT          260 a          263 c          319 g          226 t
ORIGIN      
        1 acagtcttgc ccagagagcc gcgctggctg cattgggaga cgatgagcat gtggccaaga
       61 gtcgggcggt taacacagct gggatggagc agatggagca ggggctgcgc gcgctgggag
      121 tgattccaat tccgagcgag accaattttc tttattttga tgccaaacgg gatgggcgac
      181 tggtgttcga agcattgctt cgagcgggga ttattgtccg acatatcgat ggcaccatgc
      241 ttcgtgtcac catcggtcaa cccgatgaaa atgccgcctt cctccaggcc ttcaggaagg
      301 tcctgtctgg agacaggtaa gaactagtga gggaattatg atcatcgtgt tgaagccgga
      361 agcgtcggaa agtgaagtgg accacattat cgaccgcctg cgagatctgg gcttaaaatc
      421 gcaaatctcc accggccagg aacgcaccat catcggagtc atcggcgacg accgaatcct
      481 gcacaatcaa cccttgacgg cgcttcctgg tgttgaaagc gtcttgccga tcctggcgcc
      541 ctggaagctg gtcagccgcg aatttaagaa ggaggcaacc atcatcgacg tcggtggcgt
      601 caaaatcgga gccaaaaaac tggccatcat ggcgggaccg tgcgcagttg aacggcttga
      661 gctcacggtg gggatcgcac acgaagtgaa ggcttccgga gcctcgattc ttcgtggagg
      721 ggcctataag cccagaacat caccgtattc ctttcaagga ttgggtcgtg aggggttgga
      781 ttacttggtc gaagcaagaa aacaaaccgg gttaccggtc gtcagtgaga tcttggatac
      841 gagagacatt gagcttttcc tcgagaaggc ggatatcatt cagatcggcg ctcggaacat
      901 gcaaaacttc gagctcctca aggaggtcgg cgcgtacgac aagccagtcc ttttgaaacg
      961 aggtctgtcg gcaacgatca aagagtttct cttgtcggca gagtacatca tgtcacgcgg
     1021 caaccaaaac gttatgctgt gtgagcgggg cattcgcacg ttcgaaac
//
LOCUS       DASTWE010001010         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_770623, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001010 DASTWE010000000
VERSION     DASTWE010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770623"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VFT30_10560"
     CDS             complement(<1..697)
                     /locus_tag="VFT30_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU5093120.1"
                     /translation="MAKRKYQGKVPLHDKYGPEAKYAVEAEALLPTTKYEEEVARGLE
                     LGLPGADSIKDRRIPTFSRGELPHFAGINTFIKAPYVEDVRKCGQYDVAILGAPFDGG
                     TTYRAGTRFGPQGIRKISALYGTYSFELGVDLRESISICDVGDVFTIPGNIEKTFDQV
                     SKGVGHVYASGAFPVVLGGDHSLGFATVRGVAQNMNGKKLGILHFDRHVDTQETDLDE
                     RMHTTPWFHATNIP"
     gene            complement(768..>1108)
                     /locus_tag="VFT30_10565"
     CDS             complement(768..>1108)
                     /locus_tag="VFT30_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093121.1"
                     /translation="TGGLYAMFAMTTGVGLYTRLTGRWDPSLGQLKLGAWGLLINTAA
                     FLWSVFEFVNIAWPRPYATSPDAPWWQLWAVPLVLGSILGLTTLYLLIGKSTRDNLLV
                     RPKESAPTQE"
BASE COUNT          250 a          307 c          306 g          245 t
ORIGIN      
        1 tggggatatt tgtcgcgtga aaccagggcg tggtatgcat acgttcatcg agatcggtct
       61 cttgcgtgtc cacatgccgg tcgaagtgga ggatgccgag cttcttgccg ttcatattct
      121 gagccactcc ccgcacggtg gcaaagccca gggaatggtc cccaccgagt accacgggaa
      181 acgcgccgct ggcatagaca tggcccacgc ccttgctgac ttgatcgaag gtcttctcga
      241 tgttgccggg aatcgtaaac acgtcgccga catcgcagat tgaaatggat tcccgcagat
      301 ccacgccgag ttcgaagcta taggtgccat acagcgccga gatcttgcga atcccttggg
      361 ggccaaaccg ggtgccggcc cgataggtag tcccgccatc gaacggcgcg ccgagaatgg
      421 ccacatcgta ttggccgcat ttacggacat cttcgacata cggagccttg ataaaggtgt
      481 tgatgccggc gaagtgagga agctcccctc ggctgaatgt cgggatacga cgatctttga
      541 tggaatcggc gccaggcaag cccaattcaa ggcctcgggc aacttcttcc tcatatttcg
      601 tggtcggtag cagcgcttcc gcttcgaccg cgtacttcgc ctccgggccg tatttgtcat
      661 gcagtggaac cttgccttgg tattttctct ttgccatcac gaactccttt ctgggagata
      721 gatgaattga tgactgcaac acgaaactca gcgaactaac ctgcttttca ttcttgagtc
      781 ggagcggact cctttggacg cactaataag ttgtctcttg tagatttacc gatgagaaga
      841 tagagcgtgg tcaaccctaa gatgcttccg aggaccagcg gtacggccca gagctgccac
      901 cagggagcgt cgggagaagt tgcatagggg cgtggccatg cgatattgac aaattcaaac
      961 accgaccaca gaaacgccgc cgtattgatc agtaatcccc aggcacctag cttcagttga
     1021 cctaacgagg gatcccagcg gcctgtgaga cgggtgtaga gtccaacacc ggtagtcatt
     1081 gcaaacatgg catacaaccc tcccgtgc
//
LOCUS       DASTWE010001011         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1287084, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001011 DASTWE010000000
VERSION     DASTWE010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287084"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFT30_10570"
     CDS             <1..498
                     /locus_tag="VFT30_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093122.1"
                     /translation="HFFSITLTGYKLPPTPPNSSARFATLRGPFSRLVDERGTTYQRG
                     IPQSISADDAQLLSYPPFTEHFLLTSEPVKLDNQDPRWTSVLPAQVPCTWQGDYALLA
                     GPFVEVADDDHHVYRRGEPLEICSKTVAVLEASGYQPHFVLLNRAGERISSEAVTCSP
                     DGACC"
     gene            498..854
                     /locus_tag="VFT30_10575"
     CDS             498..854
                     /locus_tag="VFT30_10575"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5093123.1"
                     /translation="MKTSINPHMLTLSQCAMVLKALGDETRLRILESLLVGEKCVTNL
                     VRELRCSQPHASHHLRILRDSGLVEGHREGKQVCYRISPAIQRALENRQGQALDFGCC
                     ELRFSETVLMTIDHAR"
     gene            901..1938
                     /gene="arsS"
                     /locus_tag="VFT30_10580"
     CDS             901..1938
                     /gene="arsS"
                     /locus_tag="VFT30_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671343.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEU5093124.1"
                     /translation="MALTLLGRHNPLSSPSEQLKVLAQPGPRLPFEAQLDQAGLYPLR
                     ATGITVLQINVGKLCNQTCRHCHVDAGPDRSEAMSLETAEQCIAALAKTDIPTVDITG
                     GAPELNPHFRWLVEQSRSLDRHVMDRCNLSVLLLPSQADLVEFLADHRVEIIASLPSY
                     RAGQTDAQRGDGVFEKSIEALRLLNRFGYGRADSGLILNLVYNPVGAFLPPKQEAIEV
                     QFKKELRTRHDVEFNRLYTITNMPISRFLEFLVESGNYEQYMTRLANAFNPAAAAGVM
                     CRYTLSVGWDGRLYDCDFNQMLELPVDHGVPSHIRDFDPAQLHDRQIVIRNHCYGCTA
                     GSGSTCGGSVA"
     gene            1974..>2266
                     /locus_tag="VFT30_10585"
     CDS             1974..>2266
                     /locus_tag="VFT30_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5093125.1"
                     /translation="MYRFIDPCRWRTCVSLTASLIVAVFGIPGLAPAQTATADPLSGK
                     LVITGASTLAPLIGEIGKRFESLYPRVRVDIQTGGSSRGVADTRQGLADIGMVS"
BASE COUNT          490 a          662 c          585 g          529 t
ORIGIN      
        1 catttctttt ccatcacgtt gaccggttac aagctgcctc cgacgccacc aaactcttcg
       61 gcccgcttcg ccacgcttcg aggtcccttc agtaggcttg tggatgagcg aggcacgaca
      121 taccagcgtg gtattccgca atcaattagc gcggacgatg cacaattatt gagctatcct
      181 ccattcaccg aacattttct cctgaccagc gagcctgtca aactggacaa ccaagatccc
      241 cgctggacat ccgtgcttcc cgcgcaggtt ccctgcacct ggcaaggcga ttatgcattg
      301 ctcgcaggcc ccttcgtcga agtagccgat gacgaccacc atgtctatcg acgaggtgag
      361 ccgctggaaa tctgctccaa aacggtggca gttctcgaag cttcagggta ccaaccccat
      421 ttcgtcctgt tgaatcgggc cggagagcgc ataagcagcg aagctgtgac ctgctcgccg
      481 gatggagcct gctgctgatg aagacctcca tcaatccgca catgctgact ctgagtcagt
      541 gcgccatggt tctcaaggcc ttgggcgatg aaacacgact acgtattttg gagtccttgc
      601 ttgtcggtga aaaatgcgtg acgaaccttg tacgagagct caggtgctct caaccccacg
      661 cttcccatca tcttcgtatc ttgcgggact ctggcctggt cgaggggcat cgggagggca
      721 aacaggtttg ttacagaatc tctcccgcca ttcaacgcgc tctggagaat agacagggac
      781 aagccctgga tttcggttgc tgtgaattgc ggttttcgga gacagtcttg atgacgatcg
      841 atcatgcccg ctgaggatga tcgattttga gtcgccttcc ccattctgaa gggccgcgat
      901 atggcgctga ccctgctggg acgacacaat cccctttcct caccttctga acagctcaag
      961 gtgctggcac aacccggccc tcgccttcct tttgaagcgc aactggatca agccggctta
     1021 tatccgctac gtgctaccgg tatcacggtc ttacagatca acgtcggcaa actttgcaat
     1081 cagacctgtc gccattgcca cgtggatgcc ggtccggatc gttccgaagc catgtctctg
     1141 gaaacagctg aacaatgtat tgcggctttg gcaaaaaccg atattccgac ggtggatatc
     1201 acaggaggcg cgccggaatt aaaccctcac ttccgttggc tcgtcgaaca gtctcgaagt
     1261 cttgaccgac atgtgatgga tcgctgtaac ctctctgtgc tactgcttcc atcacaggca
     1321 gatttagtcg aattcctggc cgatcaccgt gtcgagatca tcgcctcgct tccctcgtac
     1381 cgtgccggcc aaaccgatgc acagcgaggc gatggcgtct ttgagaaatc gatcgaggca
     1441 ctccgtctct taaatcgttt cggctatgga cgagcggaca gtgggctcat cctgaacctc
     1501 gtctacaatc ctgtcggtgc attcctacct ccgaagcaag aagccatcga agtgcagttc
     1561 aagaaagagc tacggacccg gcacgacgtc gagtttaacc gcctctacac gattacgaac
     1621 atgccgatca gccgcttcct ggagttcctc gtcgaaagcg gcaactacga acagtacatg
     1681 acgcgcctgg ccaacgcatt taatcccgcc gccgccgccg gcgtgatgtg ccggtatacc
     1741 ctgtcggttg gatgggacgg gaggctctac gattgcgatt tcaaccaaat gttggaacta
     1801 ccggtcgacc atggagtgcc atcgcatatc cgagacttcg atcctgcgca actccatgat
     1861 cgtcaaattg tgatacgtaa ccattgctac ggttgcacgg caggttccgg ctcaacgtgt
     1921 ggtggttcgg tcgcttgaat aatcaccttc gactcatata tgtagttgtc tacatgtata
     1981 gatttattga tccatgtcga tggagaacgt gtgtttcgct caccgcgtcc ttgattgtcg
     2041 ccgtcttcgg gattccggga ttagcaccag cccaaacggc cacagcagat ccactgtccg
     2101 gcaaactggt aatcaccggt gcaagtactt tagctccact gatcggcgag atcggcaaac
     2161 gattcgagag tctctatccc agagtgcgtg tggacatcca gacgggcggc tcctcgcgcg
     2221 gggtcgctga cacacgccag ggactcgccg atatcggtat ggtctc
//
LOCUS       DASTWE010001012         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_608232, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001012 DASTWE010000000
VERSION     DASTWE010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608232"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFT30_10590"
     CDS             complement(<1..869)
                     /locus_tag="VFT30_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093126.1"
                     /translation="MNIANSFQALAENDNTAPGIVSNFHINSQNGRTLEVGWTASGDD
                     SVTGQASLYQLNFTDGGSGTVIPLKGLVPMPSGKGQVATVTIPYRHTTGTLSVVPFDN
                     VGNVGPTMTLPVGVQQSAGDPYTFATGAAAGLSAGGQKLSLNSDDEYVETLLPFSFPF
                     FGQNFTEVTISTNGALYFGEPPPIRDDGTADDVPSSPGKLGGYRAVAGLWDDLDLRTS
                     SRADAGVFQVINANQVIYRYLAKPCNFDGNVCTGGADVNFEIELNSNGVIKMRYGSGN
                     TQLFPTVGIGGAG"
BASE COUNT          246 a          283 c          273 g          233 t
ORIGIN      
        1 tgaccggcgc caccaattcc gactgttgga aacaactggg tgttgccact gccgtaacgc
       61 atcttgatca cgccgttcga gttcaattcg atctcgaagt tgacatcggc gcccccggta
      121 caaacgttgc cgtcaaagtt gcagggtttc gcaaggtaac gataaatcac ctggttcgca
      181 ttgatgactt gaaagacacc cgcgtcagca cgactcgagg tccgcagatc caggtcgtcc
      241 cacaatcccg cgacagcgcg ataacctccg agtttgcctg gtgaactggg gacgtcgtca
      301 gccgtaccgt catcacgaat cggtggcggc tctccgaagt acaaagcacc atttgtagag
      361 atcgttactt cggtaaagtt ttgaccaaag aatggaaaac tgaatggcag taatgtttcg
      421 acatactcat cgtcagagtt caggctcaac ttctgtcctc cggctgagag tcctgccgcc
      481 gctcctgttg cgaaggtata tgggtcacca gccgactgct gcacgccaac tggaagcgtc
      541 atcgttggac caacgtttcc gacattatca aatggcacca cactcagggt tccagtggta
      601 tgccggtaag gaatggtaac ggttgcgacc tggcccttgc ctgatggcat cgggactaag
      661 cctttcaacg gaataacggt accggaaccg ccgtcagtaa aattcaattg atacaaagaa
      721 gcctgacccg tcacgctgtc atcacctgag gcggtccaac ccacctctaa tgtgcggccg
      781 ttctgtgaat tgatatggaa gtttgaaacg atgccggggg cggtattgtc gttttccgcg
      841 agcgcctgga agctattagc aatattcaaa cggcgacctg tgagtgtcgt atcgactagc
      901 gtcggtcgga catcaccatt ggatagcaac agtcccttca cttgggcagg agtcagattt
      961 ggattctgcg cccacagcaa tgcggccgcg cccgacacat ggggcgtaga catcgacgtc
     1021 cctttgaaga acgta
//
LOCUS       DASTWE010001013         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1319547, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001013 DASTWE010000000
VERSION     DASTWE010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319547"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..980
                     /locus_tag="VFT30_10595"
     CDS             135..980
                     /locus_tag="VFT30_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5093127.1"
                     /translation="MKLGAFEIYPVSDGRFRLDGGAMFGVVPKVLWEKCCPSDEFNRI
                     SLSLTALLIRANGKNILVDTGLGTKEDEKFHRLFAVERTPTILESLKLLGLGPEDIHL
                     VINTHLHFDHAGGNTMSGGGDGSIRPVFPKARYVIQQGEFEDAARANERTRASYRPDN
                     FTPIDHINQWEFLHGDTEIVPGVTAVVTSGHTRHHQSVKIESEGQIAFFLGDLIPTVS
                     HLPLPYIMGYDLFPIQTLETKRWVLDRAFEERWLLLFEHDPVVQAGYVRRDQDGKYFL
                     REVTA"
     gene            977..1384
                     /locus_tag="VFT30_10600"
     CDS             977..1384
                     /locus_tag="VFT30_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5093128.1"
                     /translation="MTVASAPLRILIGKVGLDGHDRGVKLIARALRDAGMEVIYTGLH
                     QTPEQVVNTAIQEDVDAIGLSILSGAHNTLFRRVLELLKEQDAEDIALFGGGIIPDED
                     VTTLTADGVKALFRPGAPMQEIVTFVKGLKPHG"
BASE COUNT          325 a          347 c          418 g          314 t
ORIGIN      
        1 aatttcagcg caatggcgct ccgctcgagg cccatccctt ccagctgagc cgttaggggg
       61 agagggagag ggacttgatc gtctgatgta cacatttgag gtgtctcacc cactgctgca
      121 agagggagag aaacgtgaag ctcggggcgt ttgaaattta tcccgtgagt gacggccggt
      181 ttcggctgga cggtggagct atgtttgggg tcgtgccgaa agtgttgtgg gaaaaatgtt
      241 gtccctccga tgagttcaat cgaatctctc ttagcctgac ggctctgctg atccgagcga
      301 atggcaaaaa tattctggtg gacactggac tgggaacaaa ggaagacgag aagtttcacc
      361 gcctgttcgc cgtcgagcgc acaccgacga tactcgaatc tctaaagctt ttaggtttgg
      421 gaccggagga cattcatctg gtgatcaata cgcatctgca ttttgaccat gccgggggca
      481 atacaatgag tggaggaggg gatggaagta tcaggccggt cttccccaag gcgcgttacg
      541 tcatccaaca gggtgaattt gaagatgctg cccgtgccaa tgaacggacg agggccagtt
      601 accgtccgga caactttacg ccgatcgatc acatcaatca gtgggaattc ctgcatggtg
      661 atacggaaat cgttccaggc gtcactgcgg tcgtcacctc cggccatact cgccaccatc
      721 agagcgtcaa gatcgaatcg gaagggcaga tcgcattttt tcttggagat ctgattccga
      781 ccgtatcgca tctgccgctt ccctacatca tgggctacga tctctttccc attcagaccc
      841 tggagacgaa acgatgggta ttggatcggg cgtttgaaga gcgatggctg ctgcttttcg
      901 aacacgatcc agtagttcag gcgggatatg ttcggaggga tcaggatggc aaatattttc
      961 ttcgtgaggt gacagcatga cagtagcgag cgcgcctttg cggattctga tcggcaaagt
     1021 cggcctcgac gggcatgacc ggggcgtgaa gctcatcgca cgggcattgc gagacgccgg
     1081 gatggaagtc atctataccg gtctgcatca gacgccggaa caggttgtca atacggcgat
     1141 ccaagaggat gtggatgcga tcggtttgag cattctttca ggggcgcaca atacgctgtt
     1201 ccgacgcgtt ctcgaactcc tcaaagaaca ggatgcagaa gacatcgcct tgttcggcgg
     1261 cggaatcatt cccgatgaag atgtcacgac gctcacagcg gacggtgtga aggccctgtt
     1321 caggcccggc gcgcctatgc aggagatcgt gacattcgtc aaagggctca aaccacacgg
     1381 ctgaagaacc atgcgtatgt tgac
//
LOCUS       DASTWE010001014         1154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_737794, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001014 DASTWE010000000
VERSION     DASTWE010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737794"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFT30_10605"
     CDS             complement(<1..203)
                     /locus_tag="VFT30_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093129.1"
                     /translation="MMLLNPGPVNVSERVRQALLRPDICHRESDFAELQHGIQSKLLK
                     AFVPGAEADYTAVLITGSGTAAV"
     gene            complement(235..813)
                     /locus_tag="VFT30_10610"
     CDS             complement(235..813)
                     /locus_tag="VFT30_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5093130.1"
                     /translation="MRPEQGTLISRAQAMAALLELLTDQPLIVCNGFPSREVQKIADR
                     STHFYMIGSMGNASAIALGVALAKPHKQVVTFDGDGNVLMGMGTLATIGALRPKNFLH
                     VVFDNEVYGTTGNQPTISNVVPLDKVAKAAGYVNVERALDREDLVYEFKDMLKKDGPS
                     MLLIKVNEFAEDAGRIVLDPPDITKRFMKAIE"
     gene            complement(810..>1154)
                     /locus_tag="VFT30_10615"
     CDS             complement(810..>1154)
                     /locus_tag="VFT30_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093131.1"
                     /translation="MAAGAYMGGKIPAVLMQNSGLGTSLNTLISLNMIYRQPCILIVS
                     WRGQGGKDAPEHLVMGEVMPQFLDTMKIPHRTLTEKTASEDFKWVAETFMKQRIPLAL
                     FITKGVVKGLHP"
BASE COUNT          252 a          347 c          289 g          266 t
ORIGIN      
        1 tccaccgctg ccgtacccga cccggtgatc agtacagccg tatagtccgc ctcggccccg
       61 ggcacaaagg ccttcagcag tttggactgg attccgtgtt ggagttcggc aaagtccgat
      121 tctcgatggc agatgtcggg acgtaacagt gcctgccgca cacgctcaga gacatttacc
      181 ggtccgggat tcaacaacat cataatgggc gcttccgtgt aagcttgaca gtcgttattc
      241 gatggccttc ataaaccgtt tcgtgatatc cggtggatca agcacaatgc gtccggcatc
      301 ttcagcaaac tcgttgacct tgatcagcag catactgggg ccgtccttct tcaacatgtc
      361 tttaaattcg tagaccagat cctcacgatc cagcgcacgc tccacattca catagcctgc
      421 agctttggcc actttgtcga gtggcaccac attcgaaatc gtcggctggt tccctgtcgt
      481 gccatacacc tcgttgtcga agacgacatg aagaaagttt ttcggcctta acgcgccgat
      541 cgtggccaat gtgcccatcc ccatcagaac attgccgtcg ccgtcgaagg taaccacttg
      601 tttgtgcggc ttggccagcg ccacgccaag cgcaatggca gacgcgttcc ccattgatcc
      661 gatcatataa aaatgcgtcg accgatcggc aattttctgc acttcgcgcg acggaaaccc
      721 gttacagaca atcaggggct gatccgtcag caactccagc agcgcggcca tcgcctgagc
      781 acggctgatc aatgttccct gctccggtct catgggtgca agcccttcac gacccctttg
      841 gtgatgaaca acgccagcgg aatccgctgc ttcataaacg tttctgccac ccatttaaag
      901 tcttcactcg cagttttttc agtcaatgtg cggtgaggaa tcttcatcgt atcaaggaac
      961 tgcggcatga cttcgcccat gaccagatgc tcgggggcgt ccttgccacc ctggccgcgc
     1021 caagacacga tgaggataca gggctgccgg tagatcatgt tcaacgaaat cagggtgttg
     1081 agggaggtgc ctagtcccga gttctgcata agcacggcag ggatcttccc tcccatatag
     1141 gcacctgccg ccat
//
LOCUS       DASTWE010001015         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_317527, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001015 DASTWE010000000
VERSION     DASTWE010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317527"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /gene="bioA"
                     /locus_tag="VFT30_10620"
     CDS             <1..1229
                     /gene="bioA"
                     /locus_tag="VFT30_10620"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEU5093132.1"
                     /translation="EGKKYLDGTSSIWVNLHGHRHPMLNLALKKQLDKIAHSTFLGLS
                     NPPAIELARELIGIAPKGLTRVFYSDNGSTAVEIALKMAIQYWQQRRPEAGPKNTFLH
                     LKLAYHGDTIGAVSVGNIDLFHSRFKPLLFSTLEAEPPYCYRCPLNLTYPSCRMACID
                     PIEQTLKSRHRELAGFIVEPLMQAAAGMIPQPAGYLKRIRELCTKYDVLLITDEVATG
                     FGRTGRMFACEHEGVTPDLMAISKGLTGGYMPLAATLTTDELYRGFLGTYDEFKTFFH
                     GHSFTGNPLGCSVALANLQVFRKEKTLSRLSSKIKMLTQWLSPMAELPIVGDIRQRGF
                     MVGIELVKDQKTKEPYPLSVKAGHRVASIARTKGLILRPIGNVLVLMPPLSTSTEELK
                     KMVEILKESVETLQID"
     gene            1277..2476
                     /gene="lhgO"
                     /locus_tag="VFT30_10625"
     CDS             1277..2476
                     /gene="lhgO"
                     /locus_tag="VFT30_10625"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEU5093133.1"
                     /translation="MQTCDFLVIGGGVIGLSIARELRRRRTDARVLLIEKEPSCGAHA
                     SGRNSGVLHAGFYYSPDSLKAKFTRLGNERLTSYCEEKQIPLNKCGKLVVAKDAADLP
                     SLDELFRRGQINGIELQALTEEEAKSIEPRVKTYQRALFSPRTSTVSPLQVVNAMQQD
                     ALHEGIQVQCGTAYRRRDSGKVRTNQDSIEAGYVINAAGLYADKIAMDYGFSEKYRIL
                     PFKGLYLYSDEPPGAIRTNIYPVPDLRNPFLGVHFTITADGKAKIGPTAIPALWRENY
                     EGLGNVNFREFVEVASRGLGLLTGAGFDFRRLAMEEIAKYSRSKMVSLASVLAEGVAE
                     RNYQTWGRPGIRAQLLDITKKKLEMDFVLEGDNRSMHVLNAVSPAFTCSLPFASYVCD
                     HIDKAIG"
BASE COUNT          673 a          836 c          775 g          612 t
ORIGIN      
        1 cggagggaaa aaaatatctg gatggcacat catccatctg ggtcaatctc cacggccatc
       61 gacacccgat gctgaacctc gccttaaaaa aacaactcga taaaatcgcc cactcaacat
      121 ttcttggcct ctccaaccca ccagccattg agctggcgcg cgagttgatc gggatcgcgc
      181 cgaagggact cacgcgtgtt ttctattccg acaacggctc tacggcggtg gagattgcgc
      241 tgaagatggc tatccaatat tggcaacagc ggcgtcctga ggccggccct aaaaacacct
      301 tcctccatct caaactggcc tatcatggag acacaattgg tgctgtaagc gtcggcaaca
      361 tcgacttgtt ccattcgagg ttcaagccgt tgctgttttc aaccctggag gcggaaccgc
      421 cctattgtta ccgctgcccg cttaacctga cctatccctc ttgtcgaatg gcgtgcatcg
      481 acccgattga acagaccctc aaaagccgcc atcgagagtt ggctgggttc atcgttgaac
      541 cgctgatgca ggcggctgcc ggtatgattc cgcagccggc cggttacctg aaacgaatcc
      601 gtgagctctg tacgaagtac gatgtattat tgatcaccga tgaagtggcg acgggattcg
      661 gacgaacggg cagaatgttc gcgtgtgagc acgaaggagt gacgccggat cttatggcga
      721 tcagcaaagg gctcaccggc ggctatatgc ccctggcggc aacgttaaca accgatgaac
      781 tctatagagg atttcttggg acctacgatg agttcaaaac ttttttccat ggacacagct
      841 ttacgggcaa tccattgggc tgttccgtgg ccctcgccaa tctccaagtc tttcgcaagg
      901 agaagacact gtcccgactg tcctcaaaga tcaagatgct gacccaatgg ctgagcccaa
      961 tggcggaact tcccattgtc ggtgacatcc gtcaacgtgg tttcatggta ggaatcgaac
     1021 tagtcaaaga tcagaagacc aaagagccct acccgctcag cgtcaaagca ggccaccgcg
     1081 tggcatcgat cgcccggaca aaagggctga ttttgagacc catcggtaat gtgcttgtcc
     1141 tcatgccgcc gttatcgaca tccaccgagg aattgaaaaa aatggtggaa atcctcaagg
     1201 aatcggtgga gactctgcag attgattgag cgtcgcttcg tgctccgcgc atccaccggg
     1261 agaaccgccg aaccacatgc agacctgtga ttttctcgtc attgggggcg gggtgatcgg
     1321 cctgagcatc gcccgggaac tccgtagacg tcggacggac gcccgtgtgt tgttgatcga
     1381 aaaggaaccc tcgtgcggag cccatgccag cgggcgcaat agcggagtgc tccatgccgg
     1441 cttttattac tcgccggaca gcctcaaagc gaagtttacc cgccttggaa acgagcgact
     1501 cacctcctat tgtgaagaga agcaaatccc cctcaataaa tgcggcaagc tcgtcgtcgc
     1561 gaaggatgcc gcggatttgc cctcgctcga tgaattattc cgtcgtggac aaatcaacgg
     1621 cattgagctc caagctctta cggaagagga agccaagtcc atagaaccgc gtgtgaaaac
     1681 ataccaacgc gctctctttt cgcctcgcac ctcgacggtc agtccactcc aggtcgtcaa
     1741 cgcgatgcaa caagatgcac tccatgaggg tattcaggtc cagtgcggca cggcctaccg
     1801 aagacgagat agcgggaaag ttcggacgaa ccaggacagc atcgaagcgg gatatgtgat
     1861 caatgccgcg gggctgtatg ccgacaaaat tgccatggac tatggattct cggaaaaata
     1921 tcgcatcctg ccctttaagg gcctctacct ctactccgat gaaccaccgg gtgcgatccg
     1981 caccaatatt tacccagttc cggatctcag gaatccgttt ctgggtgtcc acttcacgat
     2041 cacggccgac ggaaaggcca agatcggtcc gacagccatc ccggctttgt ggcgcgagaa
     2101 ttatgagggg ctcggtaacg tcaacttccg tgaattcgtc gaggtcgcga gccgaggact
     2161 cgggctactg actggggctg gattcgattt ccgacgcttg gcgatggagg aaatcgcaaa
     2221 atattcacga agcaagatgg tgtcgcttgc ctcggtactc gccgaaggcg tggccgaacg
     2281 taactatcaa acatgggggc gtcccggaat tagagcccag ctgctcgaca tcacgaagaa
     2341 aaaacttgaa atggattttg tcttggaagg cgataaccgc tccatgcacg tgctcaacgc
     2401 cgtgtccccc gcctttactt gttcgctccc cttcgcaagc tatgtttgcg atcatatcga
     2461 caaagcgata ggctgacgca gcagaatgtt gaaaaagccc cccagcttcg ttctcgcggg
     2521 acactgccgc ctcacaaaga caccgggcgc tcacgggaca ctgcccgctc accgttctcg
     2581 cggacgttca caaacgtgcc gcgccttatt cggcgcggcg tgaacctcgc ggccgtccgc
     2641 aaacatgaaa aaatcggaca ctgctggctc accgactcgc cggcgcgcac agactggcgc
     2701 tctttattca tcgcgccgtg cgcctcatta ttcttcgtgt cgcggacctc gctgcggcct
     2761 tgcctgtgaa aggcgcgtcc tggcgcgccg gggtggacgg gtgagaacga cggccttttt
     2821 gaatatcctg cggccattct aattcgatcc ggtcaacaga gccgtacctc ttcgccaacg
     2881 ccgacttgca ggcttt
//
LOCUS       DASTWE010001016         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1610805, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001016 DASTWE010000000
VERSION     DASTWE010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610805"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFT30_10630"
     CDS             <1..1022
                     /locus_tag="VFT30_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093134.1"
                     /translation="AETVEKRAGEVLQKYADQAGVKLHYFSYSHLKRLHDYLRELGKE
                     DLIETISLVGYSPLRNACLVAAHILNKDIAVSIDDDCLFIEPEYIGRIKSKMLSEFEG
                     KPIRAYCGPYITAKETIYLDLPTAPHAVYWNVVEVMNEAFRKYIVESPGMKEVPFAIM
                     GNIAVHRDFYTRVPLDPPLQRGEDMDWVMNSHILGERFILDTELVIKHDPPPRPYPTW
                     RPLREDIYRFRYQQAKIANSRDMDGYHAVQRERYLPYPGTFFQDDFLERVTHACTILA
                     NDYLTKNEPENARESLNNIYHAHYLAKPKNDPFQSFIAFQKKWEEMMGIIGEKRPEVQ
                     KRVFG"
BASE COUNT          272 a          282 c          287 g          247 t
ORIGIN      
        1 tggctgagac ggtcgaaaag cgcgcaggtg aagtgctgca aaaatatgcc gatcaggcgg
       61 gggtcaaact acattatttc agttattccc atcttaagcg cctgcatgat tatcttcgtg
      121 aattgggaaa agaagatctc attgaaacca tctcgctcgt tggctattca cctttgcgga
      181 atgcgtgtct ggtggcagcc catatcctga acaaggatat tgctgtgtcc attgatgatg
      241 actgtctgtt catcgagccg gaatacatcg ggcggatcaa atcgaagatg ctctccgagt
      301 tcgagggaaa gccgatccgc gcctattgcg gtccttatat cactgccaaa gaaacgatct
      361 atctcgatct tccaacagcc cctcatgcgg tgtactggaa cgttgtagag gtcatgaatg
      421 aggcgttcag gaagtacatc gtagaatcgc cagggatgaa agaggtgccg tttgccatca
      481 tgggcaatat tgctgtgcac cgcgactttt acacccgcgt gcccctggat ccgcctctgc
      541 agcgcgggga ggatatggac tgggtgatga actcacacat tctcggcgaa cgctttatcc
      601 tggatacaga gctggttatc aagcacgatc cgccgccgcg cccgtatccc acctggcggc
      661 ccctgcggga ggatatttat cgttttcgct accagcaggc gaagatcgcc aacagccgtg
      721 atatggatgg ttatcatgcg gtccagcgcg agcgttatct tccctatccc ggcacgttct
      781 tccaggacga ttttctggag cgcgtgaccc acgcctgcac catcctggcg aacgattacc
      841 tgacgaagaa tgagccggag aatgcccgcg aatcgctcaa caatatttat cacgcccatt
      901 acctggcgaa accaaaaaac gaccctttcc aatccttcat tgcgttccag aagaaatggg
      961 aggagatgat gggaatcatc ggagaaaagc gacctgaagt gcaaaaaaga gtgtttggct
     1021 gatgaaaccc gaatctctcc tcggtgtatg gacagggacc gcacacaaca gcaacggctg
     1081 ggacatga
//
LOCUS       DASTWE010001017         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_285607, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001017 DASTWE010000000
VERSION     DASTWE010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285607"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1058
                     /locus_tag="VFT30_10635"
     CDS             201..1058
                     /locus_tag="VFT30_10635"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEU5093135.1"
                     /translation="MPSAGQLVQSCTTVFTLPEIYFRVRDVVDDPDSTMDDLADVLKL
                     DPAISARLLRIVNSPLYGFPKQIDTISRAVNIVGMQAINDLVAATTVGRTFSGMPIQL
                     MDVPRFWRKSVLCALLAGKIAKSCGIEDSERFFIEGLLRDIGHFVLYQTIPQRAQSAL
                     IEAGYLETSLAEVEQSNIGCDFAEVGAELIRSWGMPLQIEQAIRCQLSPNDAGEFIIH
                     ASIVHLAGVVADYEELEPSRRPTTFPFNPHAVTATRFVPTNLPALLTDACAQLQDTLA
                     VIQPRAMAA"
     gene            complement(1212..>1406)
                     /locus_tag="VFT30_10640"
     CDS             complement(1212..>1406)
                     /locus_tag="VFT30_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093136.1"
                     /translation="IISGPYGDTVVGRRHSQLINILGLIYCDRVTISQPVLSQDYQAF
                     QLFFCSDDTGPESIGQHTVA"
BASE COUNT          312 a          387 c          372 g          335 t
ORIGIN      
        1 tcataacgga agtggggcca gtcaggttgt tgccagttat aggacatgag ccgataatat
       61 acatattcgg ctcaaatgtc accgtaagtt gagccgaatg tattctgtat tcggctcatg
      121 gggaccactt cttctacagt tctgcccgtt agtaaccgat agaaaaggcg gaagcccggt
      181 caaactgaaa ggactccacc atgccatcag cgggccaact ggtccagtct tgcaccaccg
      241 ttttcacctt gccggagatc tatttccgtg tgcgagatgt agtggatgac cccgattcga
      301 ccatggatga tctcgccgac gtcctgaagc tggatccggc tatttcggcc aggcttctcc
      361 gcatcgtcaa tagccccctc tatggattcc ccaagcagat cgacaccatt tctcgcgccg
      421 tcaacattgt cggtatgcaa gccatcaacg atctcgtcgc cgcaacgacc gtcggacgga
      481 ctttctccgg gatgccgatt caactcatgg atgtcccgag gttttggcga aaaagcgtgc
      541 tctgtgcctt gctggctggg aagatcgcca aatcctgcgg catcgaagac agcgagcggt
      601 ttttcattga aggcctgctg cgcgacattg gtcactttgt cctctaccag accattccac
      661 agcgggctca gtctgccctc atcgaagcgg gctatctcga gacgtctctg gccgaggtgg
      721 agcagtccaa cattgggtgc gacttcgcgg aagtgggggc cgaactcatc cggtcctggg
      781 gtatgcccct gcagattgaa caggctattc gctgccaact gagcccaaac gacgccggcg
      841 aattcatcat tcatgcgtca attgttcatc tggccggtgt cgtcgccgac tacgaagaac
      901 tcgagccgag tcggagacct actacattcc cgtttaaccc ccatgctgtc accgccacac
      961 ggtttgtgcc aaccaacctc ccggcactgc tcacggacgc ctgtgcccag ttgcaagata
     1021 ccttggcagt tatccagcct cgcgccatgg ccgcatagtt tctcagaata ttgccgagca
     1081 ggaataggct ccgacgcttg atgtgtgctt ttcatcgatc ttggtgaatg cacagcattt
     1141 tccgattgag caggcgtgct atcgatggga gacgggaggg acttattatt ataggatata
     1201 tctgttaggc attaagctac agtatgctgg ccaatcgatt caggcccagt gtcgtctgag
     1261 caaaaaaata gctggaatgc ctggtagtct tgggatagca ccggttggct gatggttact
     1321 cgatcacagt agatgagccc aaggatgtta atcaactgtg agtgtcgccg gccgacgacg
     1381 gtatccccgt acggaccaga aatgat
//
LOCUS       DASTWE010001018         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_867346, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001018 DASTWE010000000
VERSION     DASTWE010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867346"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFT30_10645"
     CDS             complement(<1..500)
                     /locus_tag="VFT30_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093137.1"
                     /translation="MDSEDTTPATQSAKPRILAVCPPPGIVAALQQALDVAIETVSTK
                     AAAHDLISDRQWSLLLLHHRGLAGSVEPFLEKIRRGRQGTMVPIVYCLERNMDPDLAK
                     KLVKDFGVQSLLTYPSDPREIAHHIGDLLSLPVKQGDPVAGAVSEAHGESSTSQEAQW
                     ESFKGTL"
     gene            806..>1034
                     /locus_tag="VFT30_10650"
     CDS             806..>1034
                     /locus_tag="VFT30_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093138.1"
                     /translation="MRIAVWSIVSGLMAPVSALAGSLQIQTANDHDLQSQVKGTANKV
                     IPAVVSIASTVMVRDQAFSDEALPFGLFKEPP"
BASE COUNT          219 a          272 c          311 g          232 t
ORIGIN      
        1 agagttcctt tgaagctctc ccactgtgcc tcttgactcg tggatgattc tccatgagct
       61 tccgagacag caccggcgac ggggtcgcct tgtttcacgg gaagactcag taggtctccg
      121 atgtgatggg cgatttcccg aggatccgac ggataggtga gcaggctctg cacaccgaag
      181 tctttcacca acttcttggc gaggtcaggg tccatgttcc tttccaggca atagacgatg
      241 gggaccatcg tgccttgccg acctcgccga atcttttcca ggaaaggctc aacactgccg
      301 gcaagtcccc gatggtgcag aagcaagagc gaccactgcc ggtcgctgat caggtcgtgg
      361 gccgctgcct tcgtgctgac tgtctcaatg gccacatcga gggcctgttg aagagctgcg
      421 acgataccgg ggggcgggca cacggccaag atgcgcggtt tcgctgattg cgtcgccggt
      481 gtcgtatctt cgctgtccat ctgattgtat gagtcttcaa cgaggcggga ttgattgctt
      541 catttccctt cacgcatgca tggtcgcagt tcacaacgcg tcggttctca gccgtattgc
      601 atcatcaagg ctgctctgcg gcaagaagtt tccaaaacgg tgtaggatag ggcaacgaat
      661 gcatatacga ttggcctgaa tgctcacgac gtgtagaatg ccgtcggccc cttgtccgat
      721 agtaaggaat gagaaaagtg cttatgagaa gtgtgacatg tggcaaaaga agagaggcgc
      781 accggtgaag aacaggaggc aagtgttgcg gatcgctgtc tggagcatcg tgagcggtct
      841 gatggctccg gtttcggcgc tggccggcag cctgcagatc cagacggcca acgaccacga
      901 tttgcagagc caagtcaagg gcaccgccaa taaagtcatt cctgccgtgg tgagcatcgc
      961 gtcaactgtg atggtgcgcg atcaggcttt cagtgacgaa gccttgccgt tcggtttgtt
     1021 caaagagcca ccga
//
LOCUS       DASTWE010001019         1627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_124164, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001019 DASTWE010000000
VERSION     DASTWE010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124164"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VFT30_10655"
     CDS             <1..1013
                     /locus_tag="VFT30_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093139.1"
                     /translation="AAQKQLQTEFRTNSDTPFATTDIEREPVPRDPLMPSADHESAGH
                     RAELHIGSRIYQYQWFRLPARPGEEDSIGLVLRDTTDESRLQDQLVQAEKTGSLGVLT
                     AGIGHELNNPLFGIIGLGEMIQEEQDLEHIKTCARDIVTHGRRMATIIRDFTGVTNRE
                     AFDKQVSVYLEQILDQALSTAQTTVEMKDIAIHKTYAGNTPVLVFPDQLRQALVNLVT
                     NAVQAMQGAGTLTLTTLVSDHTVTATIADSGSGISKQHLPKIFDPFFTTKGQGEGSGL
                     GLTVARRLIRKFGGDLRIESVEGRGTACILTLPTTSPMSSEGGPWTTDTAHSEPQPSH
                     SS"
     gene            962..1555
                     /locus_tag="VFT30_10660"
     CDS             962..1555
                     /locus_tag="VFT30_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU5093140.1"
                     /translation="MDNRHCAFRTATIAFFLGGLWIGIPFPSAKESPGLIGIAPEKVA
                     DYVHAVLEADRSIYTTQVVNRMQEKGIVAAAEHWEHDNALPLPAQFLQHSGRLVAESG
                     RGIRYRLIGLSPIYQRNAPATEFERKALTELQRQPDRPVTGIVSSGKKQYFQAIYSDR
                     AVSSACVTCHNSHPLSPKQDFKLNDVMGGIAITIPME"
BASE COUNT          389 a          469 c          450 g          319 t
ORIGIN      
        1 cagccgctca gaaacagctt caaacagagt tccgaacgaa cagcgatacg ccgttcgcca
       61 caaccgatat cgaacgtgag ccggtaccga gggacccact tatgcctagc gcggatcacg
      121 aatcggccgg ccatcgcgcc gaactccaca tcggatcccg catctatcag taccaatggt
      181 ttcgcttacc cgcgagacca ggagaagaag acagcatcgg actggtgctt cgagacacca
      241 cggacgagag tcggttgcaa gaccagctgg ttcaagcgga gaaaacgggc agcctcggcg
      301 tactaaccgc cggaatcggc cacgaactca acaatccctt gttcggaatc atcgggttgg
      361 gagaaatgat tcaggaagag caggacttgg aacatatcaa gacctgcgcc cgagacattg
      421 tgacgcatgg ccgtcgcatg gcgacgatca ttcgagattt taccggcgtg accaaccgtg
      481 aggcctttga caagcaggtg tcggtgtatt tggagcagat attggatcaa gcactgtcca
      541 cggcacagac caccgtggaa atgaaagaca tcgcgattca caaaacctat gcgggcaata
      601 cgcctgtgtt ggtattcccg gatcaactcc ggcaagcgtt ggtgaatctt gtgaccaatg
      661 ccgtccaagc catgcaaggc gccggcacac tcacgctgac gacactcgtg tctgaccata
      721 ccgtgaccgc gacgatcgcc gattctggct ccggcatctc caaacaacat ttacccaaga
      781 tattcgaccc gttttttacg acgaagggac agggggaagg ttcgggcctc gggctcacgg
      841 tggcgaggcg gcttatccgg aagttcggag gcgatctccg cattgagagc gtcgagggtc
      901 gcggtaccgc ttgcatcctc acgctcccga ccacgtctcc catgtcttct gagggaggtc
      961 catggacaac cgacactgcg cattccgaac cgcaaccatc gcattcttct taggcggact
     1021 ttggatcggg attccgtttc catccgccaa ggaaagcccg ggtctcattg gcattgcgcc
     1081 tgagaaagtc gccgattatg tccatgccgt cctcgaagcc gaccgatcga tttacacaac
     1141 gcaggtggtc aatcgcatgc aggagaaagg cattgtcgcg gcggcggaac attgggagca
     1201 tgacaatgcc ctcccccttc ctgcccagtt tcttcagcat tccggacggt tggtagccga
     1261 gagcggacga ggcatccggt acagattgat cgggctttct ccgatttatc aacgcaacgc
     1321 tcccgccacc gaattcgaac gaaaggccct gaccgagctc cagcggcagc cagatcgacc
     1381 ggtcaccgga attgtgtcga gcggcaagaa acagtacttc caagccatct attcagaccg
     1441 ggctgtctct tcagcctgcg taacatgcca caacagtcat ccgttgagcc cgaagcagga
     1501 tttcaagtta aacgatgtga tgggtgggat cgccattacg attccgatgg agtagcggga
     1561 cgcaccatct gtcatggtcg gctggagaac gaaccgctgt tcatgatggc gtgacaaggt
     1621 gattacg
//
LOCUS       DASTWE010001020         1925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_641163, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001020 DASTWE010000000
VERSION     DASTWE010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641163"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /gene="rapZ"
                     /locus_tag="VFT30_10665"
     CDS             complement(<1..733)
                     /gene="rapZ"
                     /locus_tag="VFT30_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU5093141.1"
                     /translation="MARLNLVIISGLSGSGKTYALKVFEDAGYFCIDNLPPALLPTFV
                     DLCNQQHSEIANVALGLDIREREFFSDFVGILERVKALGHAVHLLFLEAREEVLIRRF
                     SESRRPHPLLPHLPVLDGIRFEKERVAELRRRADRIIDTSDLTVHELGDLLAKQFQQE
                     SSDRGLTISLMTFGYKFGVPYDIDLLFDVRFLKNPFFVPELKPLSGNDPLVRTFVLTD
                     PEAVSLVEHIEGLLKFLLPLFRRERR"
     gene            complement(931..1242)
                     /gene="raiA"
                     /locus_tag="VFT30_10670"
     CDS             complement(931..1242)
                     /gene="raiA"
                     /locus_tag="VFT30_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU5093142.1"
                     /translation="MKLRITGRHMDITPALRSYVETRFGRLDRYGLKVGSLQVVLGVE
                     KLQHKAEVTGAVSGKRVQAKTSTPEMYATIDALVDRVDAQFRKWKDRLVNHKPTKPKR
                     S"
     gene            complement(1287..>1925)
                     /locus_tag="VFT30_10675"
     CDS             complement(1287..>1925)
                     /locus_tag="VFT30_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEU5093143.1"
                     /translation="YVIVPDVFVVKNEGEWVVLLNDDGLPRMRISPYYKQLISSGQGG
                     NPETKAYMDEKLRAAQWVIRSIEQRNRTIVKVVSSIVKFQERFFDHGVQYLKPLVLKQ
                     VAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADEPSGMHSSVSVRDMIK
                     TMVSEEDAKRPLKDEEIAARLRKQGVLIARRTVAKYRAELNIPSASQRKRFF"
BASE COUNT          485 a          512 c          468 g          460 t
ORIGIN      
        1 tacgccgctc tcgccgaaag agcggcaaga gaaacttaag caggccttcg atgtgctcca
       61 cgagtgaaac ggcctcaggg tcggtcagaa cgaaggtccg gaccagcgga tcgtttcctg
      121 agagaggttt gagctcagga acgaaaaacg ggttcttaag aaatcgcaca tcaaacagca
      181 aatcgatatc gtacggcact ccgaatttat acccaaaggt catgagtgag atcgtcaatc
      241 ctctgtccga ggactcctgt tgaaactgct tcgcaagcaa gtcaccaagt tcatgaaccg
      301 tcaggtcgga agtatcaatg attcgatcgg cccggcgccg gagttcggct acacgttcct
      361 tttcgaatcg gattccatcc aaaacgggga gatgtggtaa aagtgggtgc gggcgccggg
      421 actctgaaaa tcgtctgatc aatacctcct cacgggcctc aagaaaaagc aggtgtacag
      481 catgtccgag agctttcacc ctctctaaga taccgacgaa atccgaaaag aactcacgct
      541 ctctgatatc aagtccaaga gcaacgttgg caatttcgct atgctgctga ttgcagagat
      601 caacaaaagt cgggagcagg gcgggaggaa gattatcaat gcagaaatat ccggcatctt
      661 caaatacctt gagggcatag gttttacctg agccggacaa cccactgatg atgactagat
      721 tcaatctggc catatgaccg taattgcctt ggtgggtatg cctgagatac tccaattgga
      781 agtgagctct ctgcctcttc cacgattcag caacgtagaa cgcgttgagc ccctcatgga
      841 catgtccatt tcggtaggcc gatcaggcac cttaactgcg ccatggaggg gaacagcgag
      901 attccacggg agattcacgt gagaacgcgg tcacgatctc ttcggcttgg tcggcttatg
      961 attgacaaga cggtctttcc atttccgaaa ctgcgcatcc acacggtcga cgagggcatc
     1021 gattgtggca tacatctcag gagtcgatgt cttggcttgc actcgtttgc cactgactgc
     1081 accagtgact tcggccttat gctgaagctt ctcgactccc aataccactt gaagcgatcc
     1141 taccttcaac ccgtatcgat ccaggcgacc gaatcgtgtt tccacatagc ttctaagcgc
     1201 tggcgtgatg tccatgtggc gacctgtgat tctcagcttc atgcccacct ccaatttaga
     1261 cctggttgga tgcgaagcaa cggtgatcaa aaaaatcgct tgcgttgact ggcggatggg
     1321 atgttcaatt cggcccgata tttggccaca gtcctccgtg cgataagcac cccctgctta
     1381 cgaagccgag cggcaatttc ttcatctttc aagggacgct tcgcatcctc ttcagacacc
     1441 attgtcttga tcatatccct gaccgaaacg gaggaatgca tgcctgaggg ctcatcagcc
     1501 cgctgaagcc cagcgttgaa gaagaacttg agttccaaca tgccttgtgg gcaatacata
     1561 tatttattgg ccgtcacacg actgatcgtc gattcatgca ttccaatatc ctcggctacc
     1621 tgcttgagaa ccaagggctt gagatattgg acgccatggt caaaaaatcg ctcctggaac
     1681 ttgacgatgc tggaaaccac tttcacgatt gtccgattgc gctgctcgat gcttcgaatg
     1741 acccactgag cggctcgcaa tttttcatcc atatacgctt tggtttccgg atttccgccc
     1801 tgtccggaag aaataagttg tttgtaatat gggctgatcc tcatgcgtgg cagcccatcg
     1861 tcgtttaaca acacaaccca ctcaccctca ttcttgacca caaatacatc aggcacgata
     1921 acgta
//
LOCUS       DASTWE010001021         1077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1352354, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001021 DASTWE010000000
VERSION     DASTWE010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352354"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFT30_10680"
     CDS             complement(<1..911)
                     /locus_tag="VFT30_10680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEU5093144.1"
                     /translation="MQLDEPEDAEPSQTDPNGFSASAELSPAPEHETGPRSDTTAVVP
                     VTALQQYLAEVRRYPYLSKEEELQLFQEYRTHGSREAAVKLIMANLRVSVSIAAEYLH
                     TGADHMDLIQEGNVGLMQAIKKFDPSKNVRFYAYAAWWSRAYILRYLLHTFRLVKVGT
                     TQDQRKLFYNLKKEKAKLERDGFAPDTKLLADRLNVRERDVIEMDQRLGNWELSLDQP
                     IGEDQDSTLLDILPSHQEPADEQLADHQLKTLFRAKLAEFIKTLEERDEDILRNRILS
                     DSPLTLDDLGDKYGITKERTRQLEARI"
BASE COUNT          220 a          270 c          309 g          278 t
ORIGIN      
        1 atgatgcggg cttctaattg acgggtccgc tccttcgtaa tgccgtattt atcgcctaga
       61 tcatcgagcg tcaaggggga gtcggataga atgcgattcc tcagaatgtc ttcgtctcgt
      121 tcttccagtg ttttgatgaa ctcggcgagc tttgcccgga agagggtctt gagctgatga
      181 tcggcgagct gttcgtctgc cggttcttgg tgggacggca agatatcaag cagcgtgctg
      241 tcttggtctt caccgatcgg ctgatcgagc gataattccc agttaccgag gcgctgatcc
      301 atttcgatga catcgcgttc ccgcacgttc agacgatcgg cgagtaattt cgtatcaggc
      361 gcgaagccgt cccgttcgag ctttgccttt tcctttttta agttatagaa aagtttccgc
      421 tgatcctgag tcgtcccaac cttaactaat cgaaaggtat gcaataggta acggaggatg
      481 taggcccgtg accaccaggc agcataggca tagaagcgca cgttttttga ggggtcgaat
      541 ttcttgatgg cttgcatcag gccgacgttg ccttcttgaa tcagatccat gtggtcggcg
      601 ccggtatgga ggtattcggc cgcaatcgag accgacacgc gcaggttggc cataatgagc
      661 ttgactgccg cttcacggct gccgtgtgta cggtactcct gaaaaagctg aagctcctct
      721 tccttcgaca gataggggta tcggcggact tcggcgaggt actgctgtag ggcggtgacc
      781 ggcaccactg cagttgtgtc ggatcgaggg cctgtttcat gctcgggagc aggactcagc
      841 tccgcgctgg ctgaaaaccc gttcgggtcc gtctgagagg gttccgcgtc ttcaggctcg
      901 tccagttgca tgtctgtttc gtcgaggatg gacatacagg cggcagacta caccagactc
      961 ctaggtcgca acaatgctga aagcgaccca agttcctatg tgaatccgga aaccattaaa
     1021 aggagggcac ttctctcgca tgtatggtcg tggatgttcg gcattaacat tttccat
//
LOCUS       DASTWE010001022         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1190719, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001022 DASTWE010000000
VERSION     DASTWE010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190719"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFT30_10685"
     CDS             complement(<1..752)
                     /locus_tag="VFT30_10685"
                     /inference="COORDINATES: protein motif:HMM:NF027805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5093145.1"
                     /translation="MTTKDDAIRLMILDPGHFHAALIQKRMSPIIARQVHVYAPEGPE
                     LQDYLSRIQGFNGREQDPTQWESIVHASPDYLSALLRERPGNVIVTAGNNRRKTEYLK
                     AAVDAGMHVLADKPMCIDVEGWDVLKAGFDAARQNGVLLYDIMTERFEITSVLQRELM
                     QLPGVFGELHKGTPEQPSVRKESVHYLSKTVAGKPLRRPPWYFDVSQQGEGIVDVTTH
                     LVDLVMWICFPERSVSYASDVEMLQARRWPTPV"
     gene            complement(884..>1174)
                     /locus_tag="VFT30_10690"
     CDS             complement(884..>1174)
                     /locus_tag="VFT30_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093146.1"
                     /translation="TFDSWDPSEASDVAGYGYLSIVQGIDDVLHLCRETAGLAESEAL
                     RKRQELLEWWEPKRALPGQALLGTAVNEAVRLSLANDNKYVGFDARMFPQVL"
BASE COUNT          250 a          348 c          310 g          266 t
ORIGIN      
        1 acaggtgtcg gccagcgccg ggcttgcaac atctcgacgt cagacgcgta agagacggat
       61 cgttcaggga agcagatcca catcaccaga tcgaccagat gcgtcgtgac atcgactatc
      121 ccttctcctt gttggctgac gtcgaagtac cacggtggcc gtcgcaaagg ctttccggcc
      181 acggttttac tcaggtagtg gacgctttct ttcctgacgg aaggctgctc gggagtacct
      241 ttgtggagtt caccaaaaac tcccggcagt tgcatcagct cacgttgcag aacggatgtg
      301 atctcaaaac gttcagtcat gatgtcgtac aacaagacac cgttctggcg ggcggcgtca
      361 aaacctgcct tcaagacatc ccacccttca acatcgatac acatcggttt atcagccagt
      421 acgtgcatgc ctgcgtccac agccgccttg aggtattcgg ttttgcgccg gttattacct
      481 gcggtgacga tgacgttgcc tgggcgctcc cgtagcaagg cgcttaggta atcgggactg
      541 gcatgaacaa tggattccca ctgtgtcggg tcttgttcac ggccattgaa tccttgaatg
      601 cggctcaagt aatcctgcaa ttcgggccct tctggggcgt agacatgcac ctggcgagcg
      661 atgatcgggc tcatcctctt ctggatcaag gccgcgtgaa agtggcccgg atcaaggatc
      721 atcagtcgaa tggcatcgtc cttggtggtc atgaaactcc cttgaaaatc gagcccggca
      781 ttcgcgtcct cgaacaaaaa cgagtacgac ggcgagaatg agtacgctga cgaatagctt
      841 tcatctgtgt ggttgcgccg cagcggcatc ccgcgtggca gtcctacaat acctggggaa
      901 acattcttgc atcaaatccg acgtacttgt tgtcgttcgc caggctcaac cgcactgctt
      961 cattcacggc cgttccgagc agtgcctgtc cgggcaacgc tctcttcggc tcccaccact
     1021 ccaagagctc ttgccgcttc cggagcgcct ccgactccgc cagtcccgcc gtttcacgac
     1081 aaaggtgaag gacgtcgtcg atgccttgaa caatgctcaa ataaccgtag cctgccacgt
     1141 cgctcgcctc ggatgggtcc caactatcaa acgt
//
LOCUS       DASTWE010001023         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_188829, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001023 DASTWE010000000
VERSION     DASTWE010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188829"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..>1075)
                     /locus_tag="VFT30_10695"
     CDS             complement(287..>1075)
                     /locus_tag="VFT30_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5093147.1"
                     /translation="DSSEQLRRAADIRGLLVSEVLANRLGVEEGSTLDIMTPSGPAQF
                     PIVAVFYDYSTDGGKLLMDRALYQSLWHDDLVTVFPVYLSPESNIDRVRERITEQLSG
                     AAGAGLPPLVISNTELRKEILEIFDRTFLLTYVLETIAVVIAMLGIVNTLVTSVLERR
                     REFATLRAIGGSAEQIRQLVLWEATYLGVIGIALGLIGGGLLSLLLIKVINKQSFGWT
                     IQMIVPFGALVQAVMLAVIATLVAGYFPARWAARQPVIDGLREE"
BASE COUNT          254 a          307 c          283 g          231 t
ORIGIN      
        1 gcgcggcttc cagcgcttcg agatctcgcc gcaccgtccg attgtgcctg aggcagtcgg
       61 cgggcaactc attgagcatt tcttgtaggg tgagcccatg ggatgattcc agtagcctga
      121 tcaggtgcca ttggcggata acttgatcat tgcgcggcat tggttcccac tcaataagat
      181 ttaggtggtg ccttgccata tagttcgaga tgtgaaaagt ttctcaccaa aatgggaagg
      241 ctgggaacgc ttgagccgac ccttgatgcc tactaagtag tcgcaactat tcttcccgca
      301 acccgtctat gaccggttgc cgtgctgccc agcgggctgg gaagtagcct gccaccaagg
      361 tggcaatgac ggcaagcatc acggcctgaa caagcgcgcc gaagggaaca atcatctgaa
      421 tcgtccatcc gaatgattgc ttgttgatca ccttgatcag caacagggaa agcaagcctc
      481 ctccaataag acccaaagca atccctatca cacccagata ggtcgcctcc cataacacga
      541 gctgtcgaat ttgctccgca ctgccgccga tggcccgtag ggtcgcaaat tcccgacgac
      601 gttcgaggac agacgtgacc agtgtattga cgatcccaag catggcaatc acgacagcaa
      661 tggtctccag aacgtacgtc agaaggaatg tgcgatcgaa gatttcgaga atctctttcc
      721 gtaactcagt gttgctgatc acgagcggcg gaagtcccgc gccggctgcg ccgcttagtt
      781 gttctgtgat cctttctctc acgcgatcga tgtttgattc agggctcaag tacaccggaa
      841 atactgtgac cagatcatcg tgccatagcg actggtagag cgcgcgatcc atgagaagct
      901 tgcccccgtc agtcgagtag tcatagaaca cggccacgat cgggaattgc gcgggaccgc
      961 tcggcgtcat aatatcaagc gtcgatccct cctccacgcc gagccgattg gccaatacct
     1021 ccgataccaa gagaccacga atatcggccg cccgtctgag ctgttccgaa gaatc
//
LOCUS       DASTWE010001024         1421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_156698, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001024 DASTWE010000000
VERSION     DASTWE010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156698"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..>1421)
                     /locus_tag="VFT30_10700"
     CDS             complement(76..>1421)
                     /locus_tag="VFT30_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5093148.1"
                     /translation="SVLVQPQTTVAQAGNPPIDWDTATGRNIVWSVELGNEVYGSPVV
                     AGDVVYVGADNTRRLNPSFQQDAGVLVAFRATDGAFLWQDVAPRVERGLRDWLLPSTT
                     SVPYVEGDRLYYVTADCQLRCLDTHGFGDGENDGPYREEVFKDNAAADIVWQLDMPAL
                     GVFPHEACNSEVLALGDLLFVGTSNGQNEGHTRVPSPRAPSVIAVNKHSGELIWRAIG
                     PGDQVLHGNWSSPVAATVNGRMQVLYGGGDGRLRAYDAASGQEVWQFDGNPKNARWLP
                     RAGVFSRSSIIASPVFADGRVFIAMGQSPGHHNAPSFVYAISPNGQGDVTKSGLLWTS
                     RDVGRVVGTPIVKDGLLYVGDVGGTVYCLDAATGALLWKHETNDTIWGSLLLTGDRLY
                     VGNDAGLMTVLRAGRRKELLAEIQMDAPIYARPAAVGDALYLATANRLYLIAAKP"
BASE COUNT          327 a          425 c          386 g          283 t
ORIGIN      
        1 agcaccggca ctgagcgtcg tcatggatga gcgatagacg accggattat agctgttgct
       61 ttgaggcgtg tcggatcaag gctttgcggc gatgaggtac aaacggtttg cggtggcgag
      121 ataaagagcg tcgccgaccg cagccggacg cgcataaatt ggagcatcca tctggatctc
      181 cgccagcaat tcctttcgtc gtccggcacg aagaacagtc atgagacctg catcgtttcc
      241 aacataaagt cgatcaccgg tcagcaggag agatccccag atagtgtcgt tcgtctcgtg
      301 tttccacagg agtgcaccgg ttgccgcatc cagacaataa accgttccgc ccacatcacc
      361 aacgtacagc agaccgtctt tcacgatggg cgttcccact acacgaccga cgtcgcgcga
      421 agtccacagg agtccactct tagtaacatc tccttgcccg tttggactga ttgcataaac
      481 aaacgaagga gcattgtggt gcccgggaga ctggcccatc gcaataaaca cacggccgtc
      541 ggcgaagacc ggtgaagcaa tgatagagct tcgtgaaaag acacccgcgc gcggaagcca
      601 gcgggcattc ttcgggttgc cgtcaaactg ccagacttcc tgacccgacg ctgcatcgta
      661 ggcgcgcagc cggccatcgc ctccgccgta gagcacctgc atgcgaccat taacggtggc
      721 tgcgacagga ctggaccagt tgccgtgcag aacctgatct cctggtccaa tcgcccgcca
      781 gatcagttcg cccgagtgtt tgttcactgc gatcacactc ggagcgcgtg gcgacggcac
      841 gcgtgtgtga ccttcattct gaccattgga ggtaccgacg aacagtaggt cacccagggc
      901 taacacttcg ctattgcagg cttcatgtgg aaagactccc aatgcgggca tgtctagttg
      961 ccagacgatg tctgcggcag cgttatcttt gaagacctct tcacgatatg gaccatcgtt
     1021 ctcgccgtcg ccgaaaccat gcgtgtccag gcaacgaagt tgacaatcag cggtgacata
     1081 gtacaaccgg tcgccttcga catacggaac actcgtcgtc gacggcaaca gccaatccct
     1141 tagaccacgc tcgacacgcg gggccacatc ctgccaaagg aacgcaccat ccgtagcccg
     1201 aaaggcaacg agtacgcctg catcttgttg aaaggacggg ttgagtcttc gtgtattgtc
     1261 ggcacctaca taaaccacat cgccggcaac cacgggactg ccatacactt cgtttcccaa
     1321 ctcgacgctc cagacgatgt tgcgtcccgt agccgtatcc cagtcaatgg gaggattgcc
     1381 tgcttgggcc acggtcgtct gcggttggac cagaacggag a
//
LOCUS       DASTWE010001025         1271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_802866, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001025 DASTWE010000000
VERSION     DASTWE010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802866"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>1271
                     /gene="rimO"
                     /locus_tag="VFT30_10705"
     CDS             15..>1271
                     /gene="rimO"
                     /locus_tag="VFT30_10705"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU5093149.1"
                     /translation="MGFVNLGCSKNQVDSEIMLGSLVTEGFQLTSDPKQAEVVIVNTC
                     GFIEEAKEESINTILEHGHLKKTGTCRVLIAAGCLAQRYQGDLLKELPELDAVVGTGE
                     FGKIAEICRDLLAPKKRHRRLWISQPPYLYDELAPRLRLGKQHSAYVKIAEGCNRNCT
                     FCAIPLMRGTQRSRPVESIVAEARQLADEGVKEINLISQDTINYGVDLGLREGLVRLL
                     RELVKVEGLRWVRPFYLYPQQVTDDLLDLYAGEEKITKYIDMPLQHINDRMLNRMHRL
                     GNRAAIEALVDRIRTRIPGVAFRTAFIVGFPGETDAAFTELRDYVEQNEFDRVAVFLY
                     SDEEGTGAVDLEEKIEREVMDERRNAVLAIQESIATAKERARVGSILDVLIDGRSAET
                     EGVLEGRHEGLAPEIDGTVFIDEDSTS"
BASE COUNT          312 a          328 c          363 g          268 t
ORIGIN      
        1 gtacaacgac tacgatcgga tttgtcaatc ttggctgctc caaaaatcag gttgactcgg
       61 aaatcatgct cggatctctc gtgaccgagg ggtttcaact gaccagtgac ccgaaacaag
      121 ccgaggtggt catcgtcaat acctgcggct tcatcgaaga ggccaaagaa gaatcgatca
      181 acaccatcct cgaacatggg catctaaaga aaacagggac ctgccgtgtc ttgatagcag
      241 ccggttgttt agctcagcgg tatcaaggag acttgttgaa agagttaccg gagttggatg
      301 cggtggtcgg caccggggaa ttcggcaaga tcgcggagat ttgccgagac ttgttggctc
      361 ctaaaaaacg gcaccgacgt ctctggatca gccaaccccc atatctctat gacgaactgg
      421 caccccgtct tagactaggg aagcaacata gcgcctacgt gaaaatcgcc gaaggatgta
      481 atcgcaattg caccttttgc gctattccgc ttatgcgagg gacgcagcgc agtaggccgg
      541 tggaatccat tgttgccgaa gcgcgccagc tcgctgatga aggtgttaaa gagatcaacc
      601 tgatctcgca agacacgatc aactacggtg tagatctcgg tctacgcgag ggcttggttc
      661 ggctgcttcg cgaattggtc aaagtcgagg ggctgcgatg ggttcgtccg ttttacttgt
      721 acccacaaca agtcacggat gacctcctcg atctctatgc cggagaagaa aagatcacga
      781 aatacatcga catgccgctt cagcacatca atgaccggat gctcaaccgt atgcatcggc
      841 tcggtaatcg cgccgcaatc gaggccttgg tcgatcgtat ccgtacgcgt atcccagggg
      901 tggccttccg aactgccttc atcgtcggct ttcccggaga aaccgacgct gcctttaccg
      961 agctgcgcga ctacgtggaa cagaatgagt tcgaccgggt cgcggtgttt ctctactctg
     1021 atgaggaagg gacgggggcg gtcgacttgg aggaaaaaat cgagcgagaa gtcatggatg
     1081 aacgccggaa tgcggttctc gcgattcagg aatcgattgc gacagctaag gaaagggcca
     1141 gagtcggctc catcctcgac gtgctcatcg atgggcgatc tgcagaaacg gaaggtgtcc
     1201 tagaagggcg tcacgaaggg ctggctcctg aaatcgacgg gaccgtcttc atcgacgaag
     1261 actcgacctc g
//
LOCUS       DASTWE010001026         1294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1578653, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001026 DASTWE010000000
VERSION     DASTWE010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578653"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFT30_10710"
     CDS             <1..498
                     /locus_tag="VFT30_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5093150.1"
                     /translation="IEEAAKHKGMVYLRAGRPKTPVLYGPEERFRIGGSKILRQSTSD
                     VLTIVAAGVTLFEALKAHDQLKSSGIAVRVIDLYSIVPIDRNSLLESGRATQRRILTV
                     EDHYAHGGLGDAVLSAVAMEGMKVYKLAVREIPHSGKPEELVDHYGIGVRSIVDAIKQ
                     IIREQ"
     gene            775..1236
                     /locus_tag="VFT30_10715"
     CDS             775..1236
                     /locus_tag="VFT30_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5093151.1"
                     /translation="MTRRNVGLSALMMGCALLLSGGMASAEDLPPLPPVPPEYADKKM
                     PAGGWTDPKAIEEGGKIYRGEFKTEVNCSSCHGKDGAPVKKGARDLRDPKLVCRFSEA
                     FWFWRVSEGVPKTKMKANKGLLSEEQIWQVMAYENQFSHGGKPADRSCYKP"
BASE COUNT          346 a          330 c          348 g          270 t
ORIGIN      
        1 atcgaggaag ctgcgaagca taagggaatg gtctatctac gcgccggaag accaaagact
       61 cctgttctct acggaccgga ggaacggttc cgcatcggtg gcagcaaaat cctccggcag
      121 agtacatcgg atgtgctcac aatcgtggcg gctggagtga cactgtttga agccttgaaa
      181 gcccatgacc agctgaagtc atcaggcatt gccgttcgtg tgatcgacct ctacagcatc
      241 gttcccatcg atagaaatag cttgctggag agtggtcggg caacccaacg tcgaatcctc
      301 actgttgaag atcactacgc ccacggaggt cttggcgacg ccgttctcag cgctgttgca
      361 atggagggca tgaaagtcta taaacttgcc gttcgtgaaa tcccgcatag cggaaaacct
      421 gaagaactcg tcgaccacta tgggattgga gtgcggtcca tcgttgatgc aatcaagcaa
      481 atcatccgtg aacaatgaaa ttgaatcatg gaccgcccga acgaaactgt ctgcatcaga
      541 catcgccgat cgtccgacgt tcaaaacagg gattacctta cgaaacattc cttgggatca
      601 gatgtcgatg ccacgccgtc agggcatatg acccatgatg catctgatgt tttcgttttc
      661 cccgagataa aatatggtat agtgcagacc aatcgagagt gcaccgcaca ctgcgatcga
      721 ttcaagtaaa taaaaacggg taactcatcc attccaattc aataggaggt acgaatgacg
      781 agaaggaatg tgggcctctc agcgctgatg atgggatgtg cccttctgct ttcaggcggc
      841 atggcctccg ccgaagatct gcccccattg cctccagttc caccggagta tgccgacaaa
      901 aaaatgcctg ccggtgggtg gaccgatcca aaagccattg aggagggggg gaaaatttat
      961 cggggcgagt tcaagaccga ggtcaactgc agcagttgcc acgggaaaga cggcgcacca
     1021 gtgaaaaagg gcgcgcgcga ccttcgtgac ccgaaactcg tctgccgctt ctcagaggcc
     1081 ttctggttct ggcgtgtctc ggaaggtgtc ccaaagacga agatgaaggc caataagggt
     1141 cttctgtccg aggagcagat ctggcaagta atggcgtatg aaaaccagtt ctcgcacgga
     1201 ggcaagccag ccgaccgctc gtgttacaaa ccataagaca aagtttagag acgactgaag
     1261 cccagtaacg acttaccgag ctctatacaa tttt
//
LOCUS       DASTWE010001027         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_900409, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001027 DASTWE010000000
VERSION     DASTWE010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900409"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFT30_10720"
     CDS             complement(<1..832)
                     /locus_tag="VFT30_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093152.1"
                     /translation="MKHSFLDVLKPISSPKPGRDLILQSLTEGTRLVLPGGEFVKDLA
                     ADRPVLTRVIRDPNTSEFDRAVWAGCGIVTSQANATALLNITSEWRREMELTSGSFAL
                     GEWKEFAKRFGYMILWAFAQLRMDALVDAVVHIGYRNPDGQPYLYAVALYDHYEYRSF
                     TEALSEMNEFHGEKPGSDDVQNALGERPSVRQWVQHEDGKLVAQLTLTAFQLIVECDS
                     PQRLDHIKHRLAVSLGFSLHFRGETLVPPARQLSVAELMSDEPPSLVVTPEEDRTLLN
                     Q"
     gene            complement(845..1156)
                     /locus_tag="VFT30_10725"
     CDS             complement(845..1156)
                     /locus_tag="VFT30_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093153.1"
                     /translation="MKHASEQAVGFWTPRPPSLDGVLSRLESLSRKPEFRLQRELAEA
                     RALKPYLEGSAGRLVTPLEQEIELANLYLFCDYYPDDGQLTLIEQLRDIITEHISEEE
                     R"
BASE COUNT          258 a          348 c          334 g          291 t
ORIGIN      
        1 actggttgag cagcgttcga tcttcttccg gcgtcacgac caacgacgga ggttcatcgg
       61 acatgagctc ggccaccgat agttgccgag ccggcggcac caacgtctcg cctcgaaaat
      121 gcaatgaaaa tcccaacgag accgcgagcc gatgtttgat gtggtccagc cgctgcggac
      181 tgtcgcattc tacgatgagt tgaaacgcgg tgagcgtgag ttgggcgaca agcttcccgt
      241 cctcatgttg gacccattgc cgaaccgagg gccgctctcc cagagcgttc tgcacgtcgt
      301 ccgaacccgg cttttctcca tgaaattcgt tcatctcaga caaggcttcg gtgaaggatc
      361 gatattcgta gtggtcgtac agggcgaccg catacagata aggctggccg tcgggattgc
      421 gatatccgat atggacgacg gcatccacca aggcatccat gcggagctgc gcgaacgccc
      481 agagaatcat gtagccgaat cgcttggcaa actccttcca ttctcccagc gcaaaggatc
      541 cgctcgtcaa ctccatctct cgacgccatt ccgatgtgat gttgagcaag gccgttgcat
      601 tggcttgtga cgtgacgatt ccacaaccgg cccatacggc cctgtcgaac tctgaggtgt
      661 ttgggtcacg aatgactcgg gtaagtaccg gacggtccgc cgcaagatcc ttgacgaatt
      721 ctccaccggg taagaccagt ctcgtcccct cggtaagtga ttgcagtatg agatctcggc
      781 ctggttttgg cgatgagatc ggtttcagta catcgaggaa agaatgcttc aaaggatcaa
      841 gccattaccg ctcctcttcg gaaatgtgtt cggtgatgat gtcccgcagc tgttcgatga
      901 gtgtcagctg tccgtcatcc ggataataat cgcagaacag gtagaggttc gctaattcta
      961 tttcttgctc aagcggcgtg acaagccgtc cagcgcttcc ttccaggtat ggtttgagtg
     1021 cgcgtgcctc tgccagttca cgttgcaacc taaattccgg tttacgcgaa aggctttcca
     1081 ggcgcgagag cactccatcc aacgacggag gccgcggagt ccagaatcct accgcttgtt
     1141 cactcgcgtg cttcatggat ttcttgcatg acgatttctt ctgcgtcgag ccaatctcga
     1201 agcgcgttgc cttcttgacg gcctcgctgt t
//
LOCUS       DASTWE010001028         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1190985, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001028 DASTWE010000000
VERSION     DASTWE010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190985"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFT30_10730"
     CDS             complement(<1..969)
                     /locus_tag="VFT30_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEU5093154.1"
                     /translation="MNIIKALFNRLSDSLLSSVQGRFDPATEFTPVASLRLVSDKPVV
                     LPSLDSSAARRSIGHAVSQPDAVDDAIRRSQSWFFGRQHAEGYWVAELEADTTLTSEY
                     LMLRRFLDRVDPDREAKAVRYLQATQLPDGGWPIYYGGPAEISASVKAYFALKLSGVS
                     ADEPFMVRAKERILAMGGVLQANVFTKITLALFDQYDWEGVPHMPVELMLLPKKFYFS
                     IYAISYWSRAVLIPLLIVFAHRPVCRISREQGIDELYPISREEVRYWKYPPFNKDQAW
                     FTPHNFFVALDAMLKLYDRMPMRVLREKALHKAACWMVDHLKGSGGL"
BASE COUNT          437 a          390 c          397 g          325 t
ORIGIN      
        1 gagtcctccg gaccctttga gatgatccac catccaacag gctgctttat gcagcgcctt
       61 ttcccgcaac acccgcatgg gcatccggtc atacagtttc aacatcgcat ccaatgccac
      121 gaagaagtta tgcggggtga accacgcctg atccttattg aatggagggt atttccaata
      181 gcgaacctct tcgcgcgaga tcgggtacaa ctcgtcgatg ccttgttcgc gtgaaatacg
      241 gcagacgggg cgatgcgcaa atacgatgag caaaggaatg agcacggccc gagaccaata
      301 cgagatcgca tagatactga agtagaactt cttcggcaac aacatcagtt cgacgggcat
      361 atggggcaca ccttcccaat cgtactgatc gaacaaggcc agcgtgattt tggtaaacac
      421 gttggcttgc aggacaccgc ccatcgccaa aatccgttct ttggcgcgaa ccataaatgg
      481 ttcgtccgcc gacacgccgc ttaatttgag cgcaaaatat gccttgaccg atgcactgat
      541 ttccgcgggg ccaccgtaat agatcggcca tccgccgtca ggcagttgcg tcgcttggag
      601 atatcggaca gccttcgcct ctcgatcagg gtcaacccga tcgaggaatc gacgcagcat
      661 caagtattcg gaggtcaaag tggtgtccgc ttccagctca gcgacccagt acccttcggc
      721 atgttgacgg ccgaagaacc acgattgact ccgcctgatc gcgtcatcaa ccgcatcggg
      781 ttgactgacg gcgtgaccta tggaccggcg agcagcggaa gaatccaatg aaggcaggac
      841 aaccggtttg tccgaaacca accgaagcga agcaacaggg gtaaactccg tagcaggatc
      901 gaatcgcccc tgaacgctcg agagcaggct gtcagagagg cggttgaata acgcctttat
      961 gatgttcatg aatggttagg actcaaaaga gacaactgcg gtaggtgaaa gaccgacatc
     1021 ctacaatagc ctcccttcgt aaacctttcc aacactcccg aacacggtaa ggcttataac
     1081 aaacgattgg aagcgagtca agcgaaggag ctgagaagtg cctgaagtat atatattttt
     1141 taccagtgta tgggatcgag aacatccgtt caacagcatc tataactgaa cgtcttaaca
     1201 cttttgtctg atcgaggagt ggagaaccga cgggagttgg tctgccttta gataaacttt
     1261 cggcaatggt cctgggggcg agaaagatca tcaatgaaag tcacaatgct ttcccccgcc
     1321 tctattcttg tcaaagatcg ggccattatc ctttctatac cgatatacag atattggatt
     1381 tcaagacaac gatgtattta atcagacgca gaaggtggga aaagatagag tcatggagaa
     1441 ggcaagtaca caggatacca gataagagaa gggattaagt aagacaggga acaattaccg
     1501 cattaaaagg actacgagag cgatgaagct ccacaacgtg accgacagg
//
LOCUS       DASTWE010001029         1099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_189073, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001029 DASTWE010000000
VERSION     DASTWE010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189073"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1099)
                     /locus_tag="VFT30_10735"
     CDS             complement(<1..>1099)
                     /locus_tag="VFT30_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093155.1"
                     /translation="PIQSQGRVIGIIEAINPIAKSFDPDALLVMTGIGGLAGSTIQNA
                     QLFERLQAAHQRYRELFEDSIDPMLITDWEGRIIESNRQAILLSGYTSEKLHTLTIDQ
                     IHEVNWQRTGTEFEILRENRTCSYESSLHREDQSQAPIEVHARRVEFDENDAIQWILR
                     DITERKELDNLREDLTAMIYHDLRSPLANIVSSLDVLFSMVPEDDQENVQAILRIAEN
                     STDRIQRLVSSLLDVNRLESGQAVVDQKVVDTIPLIERAVLDVEPVARGRRQTVSAKL
                     PQELPPIWVDEDMARRVLINLMENSVKFTPTGGHIEAGAEHEDQWVHLWVRDNGPGIP
                     AAEQDRIFDKFTRLRGTNKSSGLGIGLAFCRL"
BASE COUNT          220 a          332 c          303 g          244 t
ORIGIN      
        1 aaggcggcag aatgccagcc cgatccccag cccgctggat ttgttggttc cacgcagtcg
       61 tgtaaatttg tcaaatatcc gatcctgctc cgccgccgga atgccgggcc cgttatcccg
      121 aacccagaga tgcacccatt gatcttcatg ctccgcgccc gcttcgatat gtcccccggt
      181 cggtgtgaac ttcacggagt tctccatcag gttgatcaga actcgccgcg ccatatcttc
      241 gtcgacccag atgggcggaa gctcctgcgg cagctttgca gagaccgttt gccggcgccc
      301 acgggcgaca ggctcgacat ccagcaccgc ccgttcgatc aatggaatgg tatccacaac
      361 cttttgatcc accacagcct gcccagattc cagtcggttc acgtcgagca gagaactgac
      421 cagacgctgg atgcggtctg tggaattctc cgcaatgcgc aggatggctt gcacgttttc
      481 ctgatcatcc tccgggacca tgctgaagag cacatccagg ctcgagacaa tattcgcaag
      541 cggagagcgc agatcgtgat agatcatcgc cgtcaggtct tcacgcagat tatcgagctc
      601 cttgcgttcg gtaatgtcac gtaagatcca ctggatcgca tcgttttcat cgaactccac
      661 ccggcgcgca tgaacttcga tgggcgcctg agattgatcc tcccggtgca gggaagattc
      721 ataggaacag gtccgattct cacgcaggat ttcgaactcc gtcccggttc gctgccagtt
      781 cacctcgtgg atctggtcga ttgtcagcgt gtgcagtttc tcgctcgtgt acccgctgag
      841 caggatcgcc tgacggttgg actcaatgat acggccctcc caatcggtga ttagcatggg
      901 atcgatgctg tcctcgaaca attcacggta gcgctggtgc gcggcctgca aacgctcaaa
      961 caactgggca ttctggatgg tggaaccagc cagtccgccg atgccggtca tgaccagcag
     1021 cgcatcgggg tcgaaggact tggcgatggg gttgatcgct tcgatgatcc cgatcacgcg
     1081 tccctgtgac tggatcggc
//
LOCUS       DASTWE010001030         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_608872, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001030 DASTWE010000000
VERSION     DASTWE010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608872"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFT30_10740"
     CDS             complement(<1..180)
                     /locus_tag="VFT30_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU5093156.1"
                     /translation="MGSLPAIARDSERGALLTVHVQPGSSRTECVGIHGDAIKIRLAA
                     RPIDGAANDELFRFIA"
     gene            complement(195..1013)
                     /locus_tag="VFT30_10745"
     CDS             complement(195..1013)
                     /locus_tag="VFT30_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEU5093157.1"
                     /translation="MTHQPREVDMTQYHILQEPFYAEVCGEIGLFTIAAERKLPVMLK
                     GPTGCGKTRFVQYMAYKLGRPLITVACHEDLTASDLVGRYLLKGQETVWMDGPLTAGV
                     KHGAIVYLDEVVEARKDTTVIIHPLSDDRRMLPIEKKGQIVEAADEFMLVISYNPGYQ
                     SVLKDLKQSTKQRFMAIEFDYPAPDIETIVVQREAGVEPAVAGNLVKLGQKVRNLRNH
                     GLEEGVSTRLLIYAGTLIKQGVPPERACDAAIARPITDDSDMQRTILEFVKAIF"
BASE COUNT          220 a          300 c          278 g          251 t
ORIGIN      
        1 ggcgatgaag cgaaacagtt cgtcgttggc ggcgccgtca atcggacgag ctgccagtcg
       61 gatctttatg gcgtcgccat gaattccgac acactcggta cgtgaagacc cgggttggac
      121 atgtacggtc aagagcgccc cgcgctcact gtcccgtgcg atggcaggaa gggaacccac
      181 gagaatcgat ggattcaaaa gatcgctttg acaaattcaa gtatcgtgcg ttgcatgtcc
      241 gaatcatcag tgattgggcg agcgatggcc gcatcacagg cccgctcggg tggtacgcct
      301 tgcttgatga gggtaccggc atagatcaag agtctggtgc tgactccttc ctccaacccg
      361 tggtttctca ggttgcggac cttttggccg agcttgacga gatttccggc gaccgcaggc
      421 tccacccccg cctctcgttg aaccactatc gtttcaatat ccggagcggg gtagtcgaat
      481 tcaattgcca tgaatctctg cttcgtgctt tgcttcagat ctttgagaac actctgatag
      541 ccggggttgt aagagatcac cagcatgaac tcgtctgcgg cctctacaat ctggcctttt
      601 ttctcgatcg ggagcattcg ccggtcatcg ctgagaggat ggatgatgac cgtcgtgtcc
      661 tttctggctt ccaccacctc gtcgagatag acaatagctc catgcttcac tccggcggtc
      721 aagggcccat ccatccatac ggtctcctgt cctttcagga ggtagcggcc caccaagtcg
      781 gatgccgtaa gatcttcgtg acatgcgaca gtgatcaacg gccggccaag cttgtacgcc
      841 atatattgaa caaaacgagt tttcccgcag cccgtcggcc ccttcagcat aacgggcaat
      901 ttcctctcgg cggcaatagt gaacaggccg atctctccac agacctccgc gtaaaacggt
      961 tcttggagaa tatgatactg tgtcatatcg acttcgcgcg gctgatgcgt cactgcatca
     1021 ctcctgattt ataacactgc ctcggcctg
//
LOCUS       DASTWE010001031         1164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_738382, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001031 DASTWE010000000
VERSION     DASTWE010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738382"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFT30_10750"
     CDS             complement(<1..979)
                     /locus_tag="VFT30_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5093158.1"
                     /translation="MVTQVLNLIFGSKNDREIKTLRPIVEQINGLEAGLTPLSDQALA
                     DKTQDFKKRLEAGQSLDDILPEAFAVCREMSRRKLNMRHFDVQLIGGMILHRGRIAEM
                     KTGEGKTLVATLPIYLNALEGKGVHLVTVNDYLAKRDAQWMSQLYHALGLSTGIIQHD
                     ASFISDPTYDASDKRLQHLRPCTRPEAYRADITYGTNNEYGFDYLRDNLVVTDLNQCV
                     QRDLNFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINAIIPQLKAEDYTIEEKT
                     KTAALTEDGNARVEKLLGVDNLYDPAHMDLVHHVVKALQAYTLYTRDVDY"
BASE COUNT          242 a          281 c          337 g          304 t
ORIGIN      
        1 catagtccac gtctcgtgta tagagcgtgt aggcttgcaa ggctttcacc acgtgatgga
       61 ccaggtccat atgggccgga tcgtacaaat tgtcgacgcc gagcagtttt tcaacgcgcg
      121 cgttcccgtc ttccgtcagc gccgcagtct tggtcttttc ttcgatggtg tagtcttcag
      181 ctttgagttg gggaatgatc gcattgatac gatagtaaag atcggtcgtc tggtcggtcg
      241 gacccgagat gatcaacggc gtccgcgctt catcgatcaa aatgctatcg acttcgtcga
      301 cgatggcgaa gttcaagtcg cgctgcacac attgattcaa atcggtgacc acgaggttgt
      361 ctcggagata gtcgaaccca tattcgttgt tggtaccata tgtaatgtcg gctcgatacg
      421 cctcgggcct tgtgcagggg cgaaggtgtt gtaggcgttt atcggacgca tcgtaggtgg
      481 ggtcagagat gaacgacgcg tcgtgttgaa tgattcccgt ggataggccc agcgcatggt
      541 agagctggct catccactgg gcatcacgtt tggcgagata gtcgttgacg gtgacgagat
      601 ggacgccttt gccttcgagc gcattcaggt agatgggaag cgtcgccacc aaggtcttcc
      661 cttcaccggt tttcatttcg gcgatgcggc cacggtggag aatcatgccg ccgatcagtt
      721 gcacatcgaa atgccgcatg ttgagcttgc ggcgtgacat ttcccggcac acggcaaacg
      781 cttcaggaag gatgtcgtct aaggattgac ctgcttcaag ccgcttcttg aaatcctgag
      841 ttttgtcggc aagggcttgg tcggagagtg gtgtgagccc agcctccaag ccgttgatct
      901 gctccacaat cggtcgcagg gtcttgattt cacggtcgtt tttgctgccg aagatcagat
      961 tgagtacttg cgtaaccata agaaaggcat tcgtcgttgt gtaagagagt tgaatgtcga
     1021 aataatcagc ccagtataca ggatctttcc agtgaatcct acccgaacct cagacggcct
     1081 catgcggctt gacaggtgtt gtcttggtca cctagtattt gttgcagggg gggaactgac
     1141 cctcatcttt ttgaagaagt gcct
//
LOCUS       DASTWE010001032         1262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1482017, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001032 DASTWE010000000
VERSION     DASTWE010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482017"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="ftsH"
                     /locus_tag="VFT30_10755"
     CDS             <1..1142
                     /gene="ftsH"
                     /locus_tag="VFT30_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5093159.1"
                     /translation="VEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRSRGAGLG
                     GGNDEREQTLNQLLVEMDGFDTKEGVILVAATNRPDVLDPALLRPGRFDRQVTVNHPD
                     LRGRSEILKVHTKNVPVAADVELEKIARGTPGFSGADLANLVNEAALWAARQNKSEVG
                     TIDFEMAKDKILMGAERRSMILTDEEKQVTAYHEAGHALMAKLLSGTDPIHKVSIIPR
                     GRALGVTMQLPTDDRHNHSKEFLYNTLAILMGGRVAEELVFKHVTTGAGNDLERATDL
                     ARKMVCEWGMSEKLGPLTFGQKEESMFLGRSFGSKRDVSDEMALEIDLEIKRLVTENY
                     ERTKRALAERMVTLKALAEALLEKEVLDALEIDQIILRTSPMVST"
BASE COUNT          318 a          291 c          387 g          266 t
ORIGIN      
        1 ttgtcgaaat gttcgtgggc gtcggggcgt cacgcgtgcg ggatatgttc gagcaagcga
       61 aaaagcatgc gccctgtatc attttcatcg atgagatcga cgccgtcgga cgatcgaggg
      121 gagcagggct cggcggtgga aacgatgaac gtgagcaaac gctcaaccag ttgctggtcg
      181 aaatggacgg atttgatacg aaggagggcg tcattctggt tgccgcgacc aatcggccgg
      241 acgtgctcga tccggccctg ctcaggccag gccgatttga tcggcaagtg acggtgaatc
      301 atccagacct tcgaggccga tcagagatct tgaaggtgca cacgaagaac gtgccagtcg
      361 cggccgacgt agagctagag aagattgctc gaggaactcc cggattctca ggcgccgact
      421 tggcaaactt agtcaatgag gctgcgctct gggctgctcg tcagaataaa agcgaagtcg
      481 gaaccataga cttcgaaatg gcaaaggaca agatcttgat gggcgccgag cgcaggagca
      541 tgattctcac ggatgaggaa aaacaggtta ccgcctacca cgaagccggc catgccttga
      601 tggcgaaact tctgtcgggc actgatccta ttcataaagt gtcgattatt ccgagaggcc
      661 gcgcattggg cgtgaccatg cagttgccga ccgacgaccg ccacaatcat tccaaagagt
      721 ttttgtataa cacgttagcc attctcatgg gcggcagagt cgcagaggaa ctggtgttca
      781 aacatgtcac aaccggtgca ggcaatgatt tggagcgtgc aacggacctt gcgcgtaaaa
      841 tggtctgcga atggggcatg agtgaaaagt tggggccgct gacgttcggg cagaaagagg
      901 aatcgatgtt tctgggacgc tcctttggga gcaagcgtga tgtcagtgat gaaatggccc
      961 tggagatcga tctagaaatc aagcgcctcg tcacagaaaa ctacgagcgg accaaacgag
     1021 ctctcgccga acggatggtg accttgaaag cgctggcgga agcactcttg gagaaagaag
     1081 tgctggatgc gttagaaatc gatcaaatta ttctacggac ttctccgatg gtttctacct
     1141 aattgtcggg tacatgtcgg ctcctattcc aggaatacga gtcggcgtca tggggggggg
     1201 aagtagaagt atgggttttt agggagtgat tgcagagcaa ccttcggatt tttcataacc
     1261 ac
//
LOCUS       DASTWE010001033         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_997599, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001033 DASTWE010000000
VERSION     DASTWE010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997599"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFT30_10760"
     CDS             <1..363
                     /locus_tag="VFT30_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093160.1"
                     /translation="MYPWEIIACVALQNHYQAIVRSPENAFNLSKFVNSYHKYTARKW
                     NTEDGLNGRKVWWNYWDTCIHSENDYCGRLRYVFWNAVKHGLVDRPEEHSFSNYAVDL
                     DQGFDATGIDEVNDVPEF"
     gene            347..>1048
                     /locus_tag="VFT30_10765"
     CDS             347..>1048
                     /locus_tag="VFT30_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEU5093161.1"
                     /translation="MFLNFKSVLDTMKIRSLETLDSGSKIYHFARENEHLGEKSRIHL
                     RIDPDGTGTLIVNANRVMHLNPTAVLMAHLLLEEKSEQEIIKSITRHYRVKARQAQSD
                     LTGFRLQLSELLRPDGVCPIHELDLETVMPFSARPSAPYRMDLAITYRCNNDCAHCYN
                     ARERNFPELHPENWKHILDQLWALGVPHIVFTGGEATLRNDLPELIAHAESNGQITGL
                     NTNARRLADPKYVQKL"
BASE COUNT          265 a          272 c          269 g          242 t
ORIGIN      
        1 atgtatccat gggaaatcat tgcctgtgtc gccttacaga atcactatca ggcaatcgtt
       61 aggtcacctg aaaatgcgtt taatttatcc aagttcgtaa atagttacca caaatacact
      121 gcgcggaaat ggaataccga agatgggttg aatggtcgta aagtctggtg gaattattgg
      181 gatacttgta ttcactctga aaatgattac tgcggtcggc tcaggtatgt gttttggaat
      241 gccgttaaac atggactggt tgacagacct gaggagcaca gcttcagcaa ttacgcagtg
      301 gatttggatc agggctttga cgctacagga attgacgagg tgaacgatgt tcctgaattt
      361 taagagtgtt ttagatacga tgaaaatccg tagtctggag actttggatt ccgggagcaa
      421 aatttatcac tttgcgcgtg agaacgaaca cctcggtgag aagtcacgga tccatctgcg
      481 gatcgatccc gatggcaccg gcaccttgat cgtcaatgcc aaccgcgtga tgcacttgaa
      541 tccaactgca gtgttgatgg cacatctcct gctggaggaa aaaagtgagc aggaaattat
      601 caaatccatc acccgtcact accgcgtgaa agccagacag gcacagagtg atctcaccgg
      661 ctttcgcctt cagctttctg aattacttcg tcccgacggg gtctgtccga tccatgagct
      721 cgacctggag accgtgatgc cgttcagcgc gcgcccttcg gctccctacc gcatggacct
      781 cgccatcacc tatcgctgca acaacgactg tgcgcactgc tacaacgccc gggagcgaaa
      841 cttccccgag ctgcatccgg agaactggaa acacattctg gatcagctct gggcactggg
      901 tgtgccgcac atcgtcttca ccggcggcga ggcgaccctg cgcaatgacc tgcccgaatt
      961 gatcgcccac gccgagtcga atggacagat caccggcttg aatacaaacg cccgccggct
     1021 ggccgacccg aagtacgtgc aaaaactt
//
LOCUS       DASTWE010001034         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_480398, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001034 DASTWE010000000
VERSION     DASTWE010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480398"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFT30_10770"
     CDS             <1..692
                     /locus_tag="VFT30_10770"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5093162.1"
                     /translation="FHGGGAISTVPRRQYVAFDVMPYLESGWNVVNIEYDVPGRTVAP
                     IAVQNGICALRWVVANAKEHGFDVDRIVTSGYSSGGWMALMTAMTAGTPAWDGPCAAS
                     ATARVAGVVSWSAPSDLPDLLEGPNAKGWTAQWFTTLPERLAIAKAVSPITLVRSGLP
                     PVLSIHGDTDGTVPYAQSVRLHEALRRAKVPEKLLTVSGGHGFYPRAEQERIFGEITT
                     FLLTHGLSPKR"
     gene            complement(799..>1076)
                     /locus_tag="VFT30_10775"
     CDS             complement(799..>1076)
                     /locus_tag="VFT30_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093163.1"
                     /translation="SIASHRMTAPGCEDTELIVRLFSSTGMVAVHGLLMPVECEVVTD
                     RDSNRPQSRQLIEKTGIAREFCSRGSRRSETAKGIEPYNRRLEKHLN"
BASE COUNT          225 a          318 c          309 g          224 t
ORIGIN      
        1 atttccatgg cggaggtgcc atcagcaccg ttccgagacg gcaatacgtt gcattcgatg
       61 tcatgccata tctcgaatca ggatggaatg tggtcaacat cgagtacgac gttccgggtc
      121 ggaccgtagc gccaatcgca gtacaaaacg gcatttgtgc ccttcggtgg gttgttgcca
      181 atgccaaaga gcatggattc gacgtcgacc ggatcgtgac ctcaggctat tcgtcgggcg
      241 ggtggatggc gctgatgacg gccatgaccg ctggcactcc tgcctgggac gggccatgtg
      301 cagcgtccgc caccgccaga gtcgccggtg ttgtaagttg gtccgctccg agtgatcttc
      361 ccgacctcct cgaggggccg aatgcaaagg gctggaccgc tcagtggttc accacgcttc
      421 cagaacgact cgcgattgcg aaagccgtgt cgccgatcac cctcgtccga tcaggactgc
      481 cgccggtgct ctccattcac ggagatactg atggaacggt tccctatgcc caatcagttc
      541 gactacacga ggccctgcgt cgtgccaagg taccggagaa gctgttgacg gtcagcggag
      601 ggcatggctt ctatcctcgc gccgagcagg agcggatctt tggcgagatc accacgttcc
      661 tgctgacaca tgggctaagc cctaagagat agcattcagc agcgctcgta acatcgatct
      721 ctctagcgac gtccggtcag gccgctgaca ctaaccagtt gcggaaatcg tcccgaacac
      781 ccagccaagc ctgagttatc agttcaagtg cttctccaat cggcggttgt agggttcgat
      841 tcccttggcc gtttctgagc ggcgcgaccc cctggaacaa aattcgcggg caatgcctgt
      901 tttctcaatg agttgacgag attgaggccg gttcgaatcc cggtcggtca ccacctcgca
      961 ctctaccggc atcaaaagac cgtgaacagc gaccatccca gtcgaagaaa ataatcgaac
     1021 gatcagttca gtgtcttcgc agcccggcgc ggtcatccgg tgcgaagcga tggagc
//
LOCUS       DASTWE010001035         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_609035, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001035 DASTWE010000000
VERSION     DASTWE010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609035"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFT30_10780"
     CDS             <1..603
                     /locus_tag="VFT30_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEU5093164.1"
                     /translation="GRQEVQALLDEVGKSHPKLVEELIPTLLPLGTVVRVLGNLLKES
                     IPIRDLRSILEAISDQAVTTKDADVLTEYARQALARTITKQYQAPDGSLQVITLDPRL
                     DRSLAEQAAALPPGATLNLDPTVSHKLLTGLKQAAERVAARGQQPIVLCSQVVRRHLR
                     RHSDRMLHAVPVMGLNEVDSFVRLQSLDTVRIDFELAQSS"
     gene            613..1956
                     /gene="flhF"
                     /locus_tag="VFT30_10785"
     CDS             613..1956
                     /gene="flhF"
                     /locus_tag="VFT30_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEU5093165.1"
                     /translation="MKVKTFHALTMQDAMRDIKEELGPDAIILSAKEVREGGRPLKAF
                     NRPVLEVMAASDQDIQQCSQGGKKMQQASPVKPPDAGSDSVLSAQTFQQTLQGVLKPN
                     GETTPLTVGKLPASLKPSMVHAKPNRLRPLRTVMNELSRLLEDLSRVDNRSIGSQPSP
                     VPGLLRRSLIEQGMNSSTVDFLLNEARKTAQAVGPSDDDSMRLALQREIAKRVRTGGQ
                     RLKGEGYPSIGLLIGPSGAGKTTAVAKLASHYRLERRRSVALITFDRCPEIAVEQLRR
                     YAKVVGVPFACALSPRQVHQGLRRHTEVDIVLIDMPGIGPGDLALATELRRLLPEEAV
                     TTHLVLSASTREQELCRITRRLGDLPHLRLLFTKLDETESFGAIFEVAHQTGVPLSYW
                     SIGRRVPGDIEVASSERLAAFLTGEHSVHSQVLVNRSVRSSEATPALMGAETHHG"
     gene            2078..>2648
                     /locus_tag="VFT30_10790"
     CDS             2078..>2648
                     /locus_tag="VFT30_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5093166.1"
                     /translation="MGKSNVVANLAVALTRLGKRVLILDADLGLGNLDILLGLVPRHT
                     IEDVLVGTHTLDEIVLKGPAGIHVLPASSGVPRLTALTEAQQLMIQEQLAQLTSEMDV
                     LLIDTGAGISPNVTFFASAADETMIVVSPEPTSLTDAYALIKVLARQYRERRFKVLVN
                     QAKSPREAAEVFGKLDVAVDHFLHVAVELV"
BASE COUNT          569 a          722 c          784 g          573 t
ORIGIN      
        1 ggccggcagg aagttcaagc actgctggac gaggtcggaa aatcgcatcc gaagctggtg
       61 gaggagctga ttcccacgct attgccgctc ggaacggtcg tcagggttct cgggaacttg
      121 ctcaaggaga gcattccaat tcgagattta cgatcgattc ttgaggctat ttcagatcag
      181 gccgtaacca ccaaggatgc tgacgtcctc acggagtatg caagacaggc gttagcccgg
      241 accatcacca agcagtatca agcgcctgac ggcagtttac aggttatcac cttagatcct
      301 cgactggatc ggtcattggc ggaacaagcg gcggcattgc cgcctggagc caccttgaac
      361 cttgatccca ccgtctcgca caagctgtta accggtctca aacaagccgc tgaacgggtt
      421 gcagcccgag ggcaacaacc gatcgtactc tgctcacagg ttgttcgccg tcatctccgg
      481 cgacacagcg atcgtatgtt gcatgccgtt cccgtcatgg gactcaatga agtggattct
      541 ttcgtccgtc tgcagtcatt ggatacggtg cggatcgact ttgagttggc gcagtcatct
      601 taaagggaag ccatgaaggt caagacattc cacgcgctca ccatgcagga tgccatgcgt
      661 gacatcaagg aagaactggg gccagacgcc atcatcttgt cggcgaaaga agtgcgtgaa
      721 ggagggcgcc ccctgaaggc attcaatcga ccggttctgg aagtgatggc cgcatccgat
      781 caagacatac aacagtgctc tcagggcgga aaaaaaatgc aacaagcttc tcctgtgaaa
      841 ccgcctgatg ccgggtcgga ttccgtgctg tccgcgcaga cctttcaaca gacgctccaa
      901 ggggtgctga agccgaatgg cgagacgacg ccactgaccg ttggtaagct gcctgcctcg
      961 ttgaagccat ccatggtgca tgcgaagccg aatcgccttc ggcctctgcg caccgtcatg
     1021 aatgaattga gccgcttatt ggaggacctg tcgcgggtgg acaatcggtc gatcggaagt
     1081 cagccgtcgc ccgtgccggg gttgctgcgt cggtcgctca ttgagcaagg gatgaattct
     1141 tctaccgtgg actttctctt gaatgaagcg cgaaagaccg cacaagccgt cggtccatcc
     1201 gacgatgatt cgatgcggct cgcgctacag cgtgaaatcg cgaagcgcgt gcgaacaggc
     1261 gggcagcgat tgaaagggga gggctatccc tccatcggtc tcctgatcgg gcccagtgga
     1321 gccgggaaga ctaccgccgt agctaagctg gcctctcatt atcgtctcga acggcggaga
     1381 tctgttgccc tcattacctt tgatcggtgt ccggaaatcg cggtcgaaca gctgcgtcgg
     1441 tatgccaagg tggtcggggt accgttcgct tgcgctctgt cgcctcgaca ggttcatcaa
     1501 gggctccgtc gccatacgga ggtagacatc gtgttgatcg acatgcctgg tatcggacca
     1561 ggtgacctgg cattggccac agaactgcgg cgacttctac cagaggaggc cgtcacgaca
     1621 catctggtcc tttcggcatc cacaagagag caggagctct gtcggatcac gaggcgcctc
     1681 ggcgatctgc cgcacctgcg tcttctgttc accaagctcg acgagacgga atcgttcggc
     1741 gccatctttg aggtggcaca tcaaaccgga gtgccgcttt cctattggag tatcggacgg
     1801 cgagtccccg gagatatcga agttgcctca tcggaacgct tggcggcgtt tctgactgga
     1861 gaacactccg tccattctca ggtgcttgtc aatcggtcgg ttcgatcatc ggaagccact
     1921 cctgcattaa tgggagcaga aactcaccat ggataatccg gttggaggtc gtgatatgcc
     1981 gttcagaaca agaaggtctg tcctaatgtc tgaggatctg agtcgaggag gcgactcgtc
     2041 tactcacgtc atagccgtct ctagcggcaa gggaggcgtg ggaaagtcca atgtggtcgc
     2101 gaatcttgcc gtggcactga cgaggcttgg aaagcgggtg ctgattttgg atgccgatct
     2161 cggcttaggc aatctcgata tcttactggg gctggttcca cgacatacga tcgaagacgt
     2221 gttggtaggc acacatacgc tcgatgagat cgtgctcaaa ggtccggctg gcattcatgt
     2281 gctcccggcc agttccggtg tcccacgcct tacggcgctg acggaggccc aacagttgat
     2341 gatccaggaa cagctggcgc agctcacctc agagatggac gtgctgttga tcgatactgg
     2401 tgcaggcatc tcgccgaacg tcaccttctt tgcctctgcg gcagatgaga cgatgatcgt
     2461 cgtctcgccg gagccgacat cactcacgga tgcctacgca ctgatcaaag tattggcccg
     2521 ccaatatcgg gaacgccgtt tcaaagtgct agtgaatcag gccaagagtc cccgtgaagc
     2581 cgccgaggtg ttcggtaagt tggatgtggc ggtggatcat tttctgcatg tggcagtcga
     2641 attggtcg
//
LOCUS       DASTWE010001036         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_286246, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001036 DASTWE010000000
VERSION     DASTWE010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286246"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFT30_10795"
     CDS             complement(<1..858)
                     /locus_tag="VFT30_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU5093167.1"
                     /translation="MWIYLNNKFVTDKEAVVSVFDHGFLYGDGVYETIRSYGPRIFMR
                     DEHLSRLFRSAAEIGLAMPIPVKNWGDILQEAMIRNEVGTDLQDAYLRITVSRGAGDV
                     GLDPALCSSPTVVVMAKPLVPPASHLYETGVNVIVASTKRNLPSALPPQIKSTNFLNN
                     IQAKREAIAAGAFDSILLNWEQHLTECTISNVFFVMNGTLRTPALECGLLDGITRSIV
                     IRLAGKLNMHIEESRYTVDQLYRADECFLTNTSMEIMPVASVDRRPVGNGKPGPLTLK
                     LREQFAAVRG"
     gene            complement(861..>1450)
                     /locus_tag="VFT30_10800"
     CDS             complement(861..>1450)
                     /locus_tag="VFT30_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEU5093168.1"
                     /translation="ERLVRLEGRRADTRPIAGTRPRGRSAFDDLRLIDELHTNEKERA
                     EHVMLVDLERNDLGRVCRFGSIHVDEFMTVEQYSHVSHLVSHVAGTLKDHATGFNLLK
                     AMFPGGTITGVPKVRCMEIIEELEPVRRGLYAGSMGYLSWSGDLDFNILIRTLVMRNA
                     VGYLQVGAGIVADSDPTREYEETIHKAQAFFSAFS"
BASE COUNT          311 a          406 c          391 g          342 t
ORIGIN      
        1 gccccgcacc gctgcgaact gctctcttaa cttcaaggta agcgggcccg gttttccgtt
       61 gccgacaggc ctacggtcga cggaagccac tggcatgatc tccatgctgg tgttcgtcaa
      121 aaaacattcg tcggcccggt acaactggtc aacggtatag cgactttctt caatgtgcat
      181 gttgagtttc ccggccaacc gaatcacgat gcttctggta atgccatcga gtaagccgca
      241 ttccagggca ggcgttcgca gagtgccgtt catcacgaag aacacattgc tgatggtgca
      301 ctcggtcaga tgctgttccc aattgagcag gatactgtcg aatgcgcctg ctgcgatcgc
      361 ttcgcgttta gcctggatat tgttcaagaa gtttgtggat ttgatctgtg gcggcaaggc
      421 gcttggtaaa ttccgcttcg tggaagccac gattacgttg acgcccgttt catagagatg
      481 ggacgctggc gggacgagcg gcttggccat tacgacgacg gtcggcgatg aacacagtgc
      541 cgggtccagt ccgacgtctc cggctcctcg ggaaaccgtg atccttagat aggcgtcttg
      601 cagatcagtc ccgacttcat tgcgtatcat cgcctcttgc agaatgtctc cccaattttt
      661 cactggaatc ggcatcgcaa ggccgatttc cgccgcggaa cggaacagcc gcgacaaatg
      721 ttcgtctcgc atgaatatcc ggggaccgta tgagcgaatc gtttcataga caccatcgcc
      781 gtagagaaat ccatggtcga agacggagac gacggcctct ttgtctgtga cgaacttgtt
      841 attcaggtat atccacatgg ctatgaaaag gcactgaaga aagcctgggc cttatggatc
      901 gtttcctcat attcacgcgt cgggtctgaa tcggccacaa ttcctgctcc tacttggaga
      961 tagcccacgg cattcctcat aaccagcgtg cgaatcagaa tattgaaatc gagatctccg
     1021 ctccagctga gatagcccat cgagccggcg tacaggccgc ggcgcacggg ctccaattcc
     1081 tcgatgatct ccatgcagcg gaccttgggt acgccggtga tggttccacc agggaacatg
     1141 gctttaagca ggttgaaacc cgtcgcgtga tccttgaggg tgccggccac atgcgagacc
     1201 aggtggctga catgagaata ttgctccacc gtcataaatt catccacatg gatgcttccg
     1261 aaacggcaga ctcgaccgag gtcgttccgc tcaaggtcga ctagcatgac atgttccgca
     1321 cgttcctttt catttgtgtg taattcgtcg atcagccgca gatcgtcgaa ggcgcttctc
     1381 ccgcggggtc tagtgcccgc aatcggtctc gtatctgctc gacgcccttc cagccgaacg
     1441 agccgttcag
//
LOCUS       DASTWE010001037         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1579078, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001037 DASTWE010000000
VERSION     DASTWE010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579078"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..>1008
                     /locus_tag="VFT30_10805"
     CDS             309..>1008
                     /locus_tag="VFT30_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5093169.1"
                     /translation="MSPKLFDTLLVLVQNSGHLVDKEELLRRVWPDSFVEEGNLTKNV
                     FLLRKILENGADVSYIETVPKRGYRFVAEVKTIQGNGSRPQVAETAELESEVRDRREV
                     EAARQNNDTKRRTPSMSRRLILAEAVIACIGIGAFLLVERQAKATRPEIRSIAVLPLQ
                     NLSGDPAQEYLADGMTEALITKLAQVRSLKVISRTSVMSYKESKKPLPSLPEIARELG
                     VDAVVEGSMQRENGR"
BASE COUNT          243 a          249 c          298 g          218 t
ORIGIN      
        1 atggtggtcc gtgtttgctc tccaaaaagc gagtctctcc ccaaaaattc agatagcttc
       61 aagaagagag cgctggagtc gaacatcttg cttttgcctg gctgctagcc gaggttgcat
      121 ctgagtccca agatggcttg acgctcctgg aaagtccttt caaacgcatc cgaaaattct
      181 ggtaaaattc ttgctagatc attcccagtt caggatcgag gttggccggg ggcaaggtgt
      241 acgagtttgg tccattccga atggattccc atgagcacgc ccgatggcgc gaggggaagg
      301 ttgttccgtt gtcacctaag ctttttgaca cccttctggt gttggttcaa aacagcggac
      361 accttgttga caaagaggaa ctgcttcgga gagtttggcc ggacagtttt gtggaggaag
      421 gaaacctgac caagaacgtc ttcctgctcc ggaagattct cgagaacggg gcagatgtgt
      481 cgtacatcga aacagtcccg aagcgaggct atcggtttgt ggcagaagta aagacgatcc
      541 aggggaatgg ttccagacct caggtagcag agacggcaga gctggaatct gaagtccgtg
      601 atcgtcgaga ggtagaagca gcccggcaga acaacgacac aaagaggcga accccttcaa
      661 tgagtcgccg gctcatactt gccgaagccg tgatcgcgtg catcgggatc ggggctttcc
      721 tgcttgtgga gcgtcaggcg aaggcgacac gccccgaaat tagatcgatt gctgtccttc
      781 cgttacagaa tctctccggc gacccagccc aggaatacct ggccgatggg atgacggaag
      841 cgttgattac caagctcgcg caagtccgct ctctcaaggt aatttcgcga acttcggtaa
      901 tgagctataa ggaaagcaag aaaccgctgc cgtcgctgcc ggagattgct cgagagctag
      961 gagtcgatgc cgtcgtcgag ggttcgatgc agcgcgaaaa cgggcgcg
//
LOCUS       DASTWE010001038         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1352917, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001038 DASTWE010000000
VERSION     DASTWE010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352917"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>1029
                     /locus_tag="VFT30_10810"
     CDS             31..>1029
                     /locus_tag="VFT30_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5093170.1"
                     /translation="MIRREYERISRVLNEFYYSLDRNGLDVDLPNKSKDLTDFRDNLD
                     EYNLTQFEAFQNNPVMVALKGSFHGKTSSALKVTFNKSYREAYEGLSAIRTVFISTDH
                     PERLEELVAEHVSVFYYPVLIGRQVTLRPIKMTRVIAFIFEILQGEGGMRPVPLHTLQ
                     YLAEVRQRVKVPFILDEIQTGCGRLGSVFAYAQTPLAQHAPEYIVVSKALGGGLVKIG
                     AALIRKDIYDQDFGILHTSTFAEDELSSRVAIKTLQLLTRNDGELLHQVREKGEYLLR
                     ELRSLQARYPNTIKEVRGAGLMIGMEFSLMDDHSPFFRSSGKQGVLSLLIASYLLEY"
BASE COUNT          261 a          261 c          259 g          248 t
ORIGIN      
        1 atcaagcacg cgtacaaggt gcaattcgac atgatacgac gggagtacga gcggatttca
       61 agagtgctca atgagtttta ttattcactc gatcgaaacg gtctcgacgt ggatttaccc
      121 aacaagagca aagacctgac cgactttcga gacaatctcg atgaatataa cctgacgcaa
      181 tttgaagcat tccaaaacaa tccggttatg gtcgccctca aagggtcatt tcatgggaaa
      241 accagctcgg ctctgaaggt gaccttcaac aagtcctatc gcgaggcata cgagggactc
      301 tcggccattc gaacggtgtt catcagcacg gatcatcccg agcggttgga agagctggtc
      361 gcggaacacg tcagtgtctt ctactatccc gtgttaatcg gcaggcaagt cactctgcgc
      421 ccgataaaga tgacccgagt cattgcgttc atttttgaaa tcctccaagg ggaaggaggc
      481 atgcggccag tccctctcca cacgctgcaa tacctagctg aggtacgtca gcgcgtcaag
      541 gtgccgttta tcttagatga aatccaaact ggatgtggcc gtctgggatc cgtattcgca
      601 tatgcccaaa cgccattggc gcagcatgct cccgaatata ttgtcgtgag taaagcgttg
      661 ggaggaggat tggtcaaaat cggcgctgcg ctgatccgaa aggacatcta cgatcaggat
      721 ttcggtattc tccacacctc caccttcgca gaagacgagt tgtcgtcacg ggttgccatc
      781 aagaccctgc agctcttgac tcgtaacgat ggagaattgc ttcatcaagt tcgagagaag
      841 ggtgagtacc tcctccgaga gttacgttcc ttgcaagcac gctatccaaa tactatcaag
      901 gaggtacgtg gcgccgggct catgatcggc atggaatttt ccctgatgga cgatcattcc
      961 ccattttttc gttcgagtgg gaaacaaggc gtcttgtcac tcctcattgc gagctattta
     1021 ctcgaatat
//
LOCUS       DASTWE010001039         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_221628, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001039 DASTWE010000000
VERSION     DASTWE010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221628"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2082
                     /locus_tag="VFT30_10815"
     CDS             <1..2082
                     /locus_tag="VFT30_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5093171.1"
                     /translation="RFVIDSQYAEQPLFKPFMKTLGVIPISSHGGLRVILKALRDAGA
                     AIDKGELVCIFPEGQITRTGTLLPFRRGFERVVKGGTAPIIPVHLDRVWGSIFSFNHG
                     RFLWKVPEQLPYPVTVSFGAPLPPDTSAHELRGKIRELGEKAWQFRKPSRRPLHRQFI
                     RSMRRYPFRMAMADLNRPRVSSLQALIGSIVLARKLRPYWKGQQRVGVLLPPTVATAL
                     VNVAAPLCGKTIVNLNYTVGKSGLEAAVELAELRTIVTSRTFVEKAKLELPDGPSVVW
                     LEDVAATITKGQKVLAALLALFAPCRFIERACGQTTPLTPDSMATIIFSSGSTGEPKG
                     VMLSHFNIDANSQGATQVLHFYQDERVLGILPFFHSFGYMVFWFVMSNNAAMIFHPSP
                     LDVAGIGELIRQHRITFIVTTPTFLQLYSRRCTPEQFSSVRVILTGAEKLPARLAEAV
                     EDKIGIGPIEGYGVTECSPVIAVNCPDFRAAGYYQPASRRGTVGQPLPGVSVQIVDPD
                     SYAPLPAGTSGMLLVKGPNVMNGYLRREDLTAQVMRNGWYITGDIASLDEDGFLTITD
                     RLSRFSKIGGEMVPHGKVEEALQQAAAADTQVFAVTGLPDDKKGERLAVLHTLEESRI
                     PPILEKISSSGLPNLFIPGKHQFVKVDALPVLGTGKLDLRGMKRIAMERLSSHETSEV
                     SKEAAENAIVE"
     gene            2079..>2434
                     /locus_tag="VFT30_10820"
     CDS             2079..>2434
                     /locus_tag="VFT30_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093172.1"
                     /translation="MTSTKSLIDCHVHLAALPDGDNGCFISPKLLRSPLFRFLLWKHD
                     LSPTHPRETNQKYLAHLLTELRASRHVSKAVLLGMDGFYDQTGLLNRQHTDFLVSNDY
                     VFKVVQTYPDVFLAGPS"
BASE COUNT          514 a          738 c          642 g          540 t
ORIGIN      
        1 cggtttgtca ttgattcgca atatgccgaa cagcctctct tcaagccctt catgaagacg
       61 ctcggagtca tccccatttc ctctcatgga ggtttgcggg tcattttgaa agcgctccgc
      121 gatgccggtg cggccatcga caagggagaa ctcgtctgca tcttccctga aggccaaatt
      181 actcgtacag gaacgttgct gccgttccgg cggggtttcg agcgagtcgt gaaaggaggg
      241 acagccccca tcatcccggt tcatctcgac cgtgtgtggg gcagcatttt cagtttcaat
      301 cacggccgct ttctgtggaa agttccggaa caacttccgt atcccgttac cgtctcgttc
      361 ggcgcaccgt tgccgccgga tacatcggca catgagctgc gcgggaagat tcgcgagctc
      421 ggcgaaaaag cctggcagtt caggaaaccg agtcgccgtc cgctccatcg ccaatttatc
      481 cgatccatgc gtcgctatcc tttccgcatg gccatggccg atctgaatcg tccccgagtc
      541 tcatccttgc aagctctcat cggatcgatc gtcctggcga gaaagcttcg gccctattgg
      601 aagggccaac aacgagtggg cgtcttgttg ccgcccaccg tcgccacagc cctggtgaat
      661 gtcgccgcgc ccctctgcgg gaaaaccatc gtgaacctga actacacagt cgggaaatcc
      721 gggctcgagg ccgccgtaga gcttgcagaa ttgcgcacaa tcgtcacgag tcgtaccttc
      781 gttgaaaaag ccaaactcga actgccggat gggccgtcgg tggtttggct ggaggatgtg
      841 gccgcgacaa tcaccaaggg tcagaaagta ctggctgccc tgctggctct gttcgctccc
      901 tgccgtttca tcgaacgcgc ctgcgggcag acaaccccgc tcacccccga cagcatggcc
      961 acgatcatct tcagcagcgg cagcaccgga gagcctaaag gcgtcatgct ttcacacttc
     1021 aacatcgacg ccaacagtca aggtgccacg caggtgctcc atttctatca ggatgaacgt
     1081 gtcctcggca ttctgccgtt cttccactca ttcggataca tggtgttctg gttcgtaatg
     1141 tccaacaatg ccgcgatgat ctttcatcct tcgcccctcg atgtggcggg aatcggcgaa
     1201 ctcatccgac agcatcggat cacctttatc gtcaccacgc cgacgttcct gcaactttac
     1261 tcccgccgct gtacgccgga acaattcagt tcggtgcggg tgatccttac cggtgcggaa
     1321 aaactaccgg cacgactcgc cgaggccgtc gaagacaaga ttggaatcgg cccaatcgaa
     1381 ggctatggag tcacggagtg ctctccggtc attgccgtca actgcccgga cttccgcgca
     1441 gccggctact atcaaccggc ctcacgcagg ggcaccgtcg gtcagccact tcccggtgtg
     1501 tcggtgcaga ttgtcgatcc tgatagttac gcaccactcc cagccggcac atccggaatg
     1561 ttgctcgtga agggaccgaa tgtgatgaac ggctatctga ggcgcgaaga tctcacggcc
     1621 caggtcatgc ggaatggctg gtacattacc ggtgacatcg cctctctgga tgaagatgga
     1681 tttttgacga ttaccgaccg gctgtcacgt ttctcaaaaa tcggtggcga gatggtcccc
     1741 cacggcaaag tcgaagaagc cttgcaacag gcggctgcag ccgacacgca agtctttgcg
     1801 gtaaccggtc tacccgacga taaaaaggga gagcgtctcg cagtcctcca tacgcttgag
     1861 gaatcccgca ttcctcccat cctggaaaag atctcgtcga gtggcctccc taatttattt
     1921 atcccgggaa agcatcagtt cgtcaaggtc gacgcgttac cggtccttgg gaccggcaag
     1981 ctcgatttac gaggcatgaa gcgcattgcg atggaacgac tctcatcaca tgagacgtcg
     2041 gaagtgagta aggaagcggc cgagaacgcg attgtcgaat gacgtccacc aaatcgctta
     2101 ttgattgcca cgtccatctt gcggccttgc ccgacggaga caatggttgc tttatctcgc
     2161 cgaaactcct tcgcagtccg ctctttcgtt ttctcctgtg gaaacacgac ctctcaccga
     2221 cccatccgcg cgaaaccaat cagaaatatc tcgcacacct gctgacggaa ctacgcgcct
     2281 cgcggcatgt ttcaaaagca gtgctgctcg gcatggatgg attttacgac cagactggcc
     2341 tgctcaatcg gcagcatacg gactttctcg tcagcaacga ttacgtcttc aaggtcgtgc
     2401 agacttaccc tgatgttttt ttggccggcc cttc
//
LOCUS       DASTWE010001040         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_835914, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001040 DASTWE010000000
VERSION     DASTWE010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835914"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VFT30_10825"
     CDS             <1..1337
                     /locus_tag="VFT30_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093173.1"
                     /translation="LDLTVQQAKGKAQREALAFLSDKKTFPLLARLLGASDFLWEDFL
                     RRQYSNLLPLLQDYRDAPLMTPKAKLRKELDRLVTRTKTDEERKAALNSFKDRELFRI
                     DMKHIVEPGTALPDFSAALTQLAEVIVDRSLKDCQAKLSKLYGPPRLANKKPCPFTVL
                     GLGKFGGRELGYASDIEVMFVYGDAGRTGGRQPIENSEYFERLGAELLQWIEAKQEGI
                     FHLDVRLRPHGGKGSLTNPFDEITSYYGDQGLAAPFERQSLIKLRHVAGDAALGKRVE
                     THRDSYVYSGKPWDLRVALDLRRQQLKQLVERGTINLKHSHGGIVDIEYAVQYLQIMH
                     GHRLPILRTSNTMQALAALVDCGIVTRQDGETLRKAYFFIRMLIDGLRMVRGNAKDRV
                     LPPVDSDEFVFLARRVGYTTDDWQAGARHLETDVQEHMGKVKEFFRRTFGKM"
     gene            1418..>2115
                     /locus_tag="VFT30_10830"
     CDS             1418..>2115
                     /locus_tag="VFT30_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU5093174.1"
                     /translation="MACYIGLSGYSYKAWQGPDRFYPSDIKPTDFLRYYATRYRTVEL
                     DGVWYRLPSENTVQTWRTDTPPHFIYAPKAHREVTHVHRLKPEALPMLMAMLQRFEPL
                     HKAGKLGPILIQLPPNLKRDDRRLETFLGSLSTTHQWAIEFRHESWQADEIERIVRQY
                     SVAWAAVETDDHEAEHRDTAPFHYVRLRKNKYSHAELRRWAERFQDASQQGQDCFVYC
                     KHEDEGSPWLWADEL"
BASE COUNT          514 a          571 c          569 g          461 t
ORIGIN      
        1 ttctggatct gacggtgcaa caggcgaaag gaaaagccca gcgggaagct ttggcctttc
       61 tcagcgataa gaagaccttt cccctgctcg ctcgtctcct gggggccagc gattttctct
      121 gggaagactt cctgcgccgc caatacagca atctgctgcc tcttctgcaa gattatcgtg
      181 atgcccctct catgactcca aaggcgaagc ttcgcaagga actcgatcgc ctcgtcaccc
      241 ggacaaagac cgacgaggaa agaaaagcgg cgctgaatag ctttaaagac cgcgaactgt
      301 tccgtatcga catgaagcac attgtcgagc cgggtactgc tctgccggat ttctccgccg
      361 cactcactca actcgccgaa gtcatcgtgg atcggagctt gaaagactgc caggccaaac
      421 tgagcaagct ctatggtcca ccgcgcctgg cgaataagaa accctgcccc ttcactgttc
      481 tcggattggg gaaattcggt ggcagagaac tggggtacgc atccgacatc gaggtgatgt
      541 tcgtatacgg tgatgcgggc cgtaccggcg gcaggcagcc gatcgagaac agcgaatact
      601 ttgaacggct cggcgcggaa ctcttgcagt ggatcgaggc caagcaagag ggcatctttc
      661 atctggacgt acgtctccgg ccccacggtg ggaaaggttc gctgacgaat ccgttcgacg
      721 aaatcacaag ctactatggt gaccagggtc ttgccgcgcc gttcgaacgt cagtcattga
      781 tcaaattgcg gcatgtggcg ggcgatgcgg ccctgggtaa gcgagtcgaa acccatcgtg
      841 atagttatgt ctacagcggc aaaccgtggg atcttcgtgt cgcccttgac ttgcgtcggc
      901 aacagttgaa gcaactcgtg gagcggggca caatcaacct aaaacatagc cacggaggga
      961 ttgtcgacat cgaatatgca gttcaatatc tccagatcat gcatggtcat cgacttccta
     1021 tcctgcgcac ctccaacacc atgcaggcgc tggctgccct cgtcgattgt ggcattgtga
     1081 cgaggcaaga cggtgaaact ctccgcaagg cctatttctt catccggatg ctcatcgacg
     1141 gactccgcat ggtccgcggc aatgccaaag atcgtgtcct tccacctgtt gattccgacg
     1201 aattcgtctt cctcgctcgc cgcgtgggct ataccaccga cgattggcaa gcgggggcca
     1261 gacatctgga aacggatgta caagagcata tgggaaaggt gaaggagttt ttcaggagaa
     1321 cgtttgggaa gatgtgactg cgggtaagga tgaagaaccg tcgttgcgca aaggttcagc
     1381 ctagtagcaa aagttttatc gcttaaggat ttaggttatg gcctgttaca tcggcctatc
     1441 aggttattca tacaaagctt ggcaagggcc tgatcgtttt tatccatccg acatcaaacc
     1501 gaccgatttc ctccgttact acgccacgag gtatcggacc gttgaactcg atggtgtatg
     1561 gtatcgactc ccaagcgaga acactgtgca aacatggcgc acagatactc ctcctcactt
     1621 catctatgca cccaaggccc atcgagaagt cacacatgtc caccgcctca agccggaagc
     1681 gcttcccatg ttgatggcca tgctgcaacg gttcgagccg cttcataagg caggtaaact
     1741 cggcccaata ctgatccaat tgccacctaa tttgaaacgc gatgatagac ggctggaaac
     1801 atttcttgga tccctgtcaa cgacccatca atgggctata gaatttcgac atgagagttg
     1861 gcaggctgac gagatcgaac ggatcgtgcg acagtattcg gtcgcatggg cggccgttga
     1921 gacggatgat catgaggcag agcatcggga cacagctccg ttccactatg ttcgacttcg
     1981 caagaataaa tacagccacg cagaactccg acgatgggct gaacgatttc aggatgcttc
     2041 tcaacaggga caggactgct ttgtctactg caaacatgaa gacgaaggct ctccctggct
     2101 ctgggcagac gaact
//
LOCUS       DASTWE010001041         1232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_738763, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001041 DASTWE010000000
VERSION     DASTWE010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738763"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..>1232)
                     /locus_tag="VFT30_10835"
     CDS             complement(115..>1232)
                     /locus_tag="VFT30_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5093175.1"
                     /translation="GMSVLPGLWDMHVHLMINGHSDYAHWDKTYPPQMEAVIMPASAR
                     QLLMAGVTSARDLGGPLKASIAVRDRIRKGEIPGPTLYVSGPFIQHEPYPGTEYVRWG
                     VKGPEDARAKVRMLADAGVDVIKLIDQDQMTMEEVQAVVDEAHKRKLPVVAHSHRPEE
                     IRRGLQVGVDCFEHTGLATAPEYPADVIALIRERTSKMSLGPLFWTPTVQGLLNYEYV
                     RDNPEQLDDPSWQVGLPQSIVDDIKQSLKKPGELGYYQITPLRRPTLARKISQLSDSG
                     VVLLIGTDSGIPMNFHSQTTWRELDAWVNGFGIPAMTAIRAATYWPSVAMKVSDQVGT
                     VSEGKYADIIAVRGDVLRHIDLLQDVDIVIKHGRRWK"
BASE COUNT          257 a          364 c          332 g          279 t
ORIGIN      
        1 agtgcgaaac gcatttctga tctttgtttc ggcccatctg taccgttgat ccgacaattc
       61 gtccattaaa ttctcatcca tagaaaccta atgcaggaaa acttggatta caccttactt
      121 ccaccggcgg ccgtgcttga tcacgatgtc aacatcctgc aagagatcga tgtgtcgcaa
      181 cacatcacca cgtacggcga tgatgtcggc gtatttgcct tcgctgacgg tgccgacctg
      241 gtccgatact ttcatcgcga ctgatggcca ataggtggcg gcgcggatgg cggtcatggc
      301 ggggatgccg aagccgttga cccaggcgtc caattcgcgc cacgttgttt ggctgtggaa
      361 gttcatcggt atgccgctgt ctgtgccgat taaaaggact acaccggagt cggaaagttg
      421 ggagatcttg cgcgcgaggg tggggcgacg cagcggtgtg atctggtagt aacccaactc
      481 accaggcttc ttcagcgact gcttgatgtc gtcaacgatc gactgtggca gcccaacttg
      541 ccacgacgga tcgtcaagct gctcgggatt gtcgcgaacg tattcgtagt tcaacaaccc
      601 ctgcacggtc ggcgtccaga acaatggtcc aagactcatc ttagacgtgc gctcacgaat
      661 caacgcgatc acatccgcgg gatactcagg cgcggtcgcg agccccgtgt gctcgaaaca
      721 atcaactcca acttgtagcc cgcgacgaat ctcttccggc cggtgtgaat gggccacgac
      781 cggcagcttg cgtttgtgtg cctcgtcgac aaccgcctgc acttcctcca tcgtcatctg
      841 atcctgatcg atcagcttga tcacgtcgac tccagcgtca gccaacatac gcaccttggc
      901 gcgggcgtcc tcgggtccct tcacgcccca acgaacatat tcagtgccgg gataaggctc
      961 gtgctgaata aacgggcctg acacgtaaag cgttggtcca ggtatctcac cttttcgaat
     1021 acgatcgcgg actgcaatgc tagctttgag tggtccaccg agatcgcgtg cactcgtgac
     1081 accggccatc aatagctgcc gcgccgacgc aggcatgatc actgcctcca tctgcggcgg
     1141 gtacgtcttg tcccagtgtg cgtagtcgct gtgaccattg atcatgagat gtacgtgcat
     1201 gtcccaaagc ccgggcaaca cgctcatgcc tt
//
LOCUS       DASTWE010001042         1871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1353085, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001042 DASTWE010000000
VERSION     DASTWE010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353085"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFT30_10840"
     CDS             <1..755
                     /locus_tag="VFT30_10840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5093176.1"
                     /translation="LDRAALLTWVNDLANVAPQGLTGLFFSNELVDSFPVHRVQVTAQ
                     GLEELWVDYRDGRFVECLRPLSSDTLAHRLRRVSADWPEGYRTEINLRASEWMKHVAQ
                     HLDRGFVLTIDYGHTAQDLYRSDRKHGTFLCYFRQSIREDPFRRVGEQDMTAHVDFSS
                     LVETGEGHKLQTTGFTNQMSFLMGLGVEQMIAELEQDSPQFKAAIHLLRPNGMGTTFK
                     VLVQHKGIQQPELDGLRYKPFFGSALTTQSAA"
     gene            809..1180
                     /gene="ndhC"
                     /locus_tag="VFT30_10845"
     CDS             809..1180
                     /gene="ndhC"
                     /locus_tag="VFT30_10845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5093177.1"
                     /translation="MGDTAVPDNYVPILIFIGVGIALGTLTLLLGKFVRPNQPYRAKL
                     APYESGSPLFQDARVQFPMRYYIIAMLFVIFDIEIVFMFPWAVAFNSLGLVGLVEMVL
                     FIAILVVGFWYAWKKGALEWD"
     gene            1225..1704
                     /locus_tag="VFT30_10850"
     CDS             1225..1704
                     /locus_tag="VFT30_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5093178.1"
                     /translation="MSLLERQFDANIVTTNLDAVVNWARKSALWPMTFGLACCAIEMI
                     ASVSSRYDLDRFGAGVFRASPRQSDLMIVAGTVSRKMAPVIRRIYDQMPEPRYVICMG
                     SCATSGNHYNSYAVVQGVDQIVPVDVYVPGCPPRPEALLDGLLKLQEKIQRERVFVK"
BASE COUNT          410 a          484 c          530 g          447 t
ORIGIN      
        1 ggctggatcg agcagcccta ctgacatggg tgaacgacct tgcgaatgtg gcccctcaag
       61 gtctgaccgg tctgttcttc agcaatgaac tcgtggattc atttccggtc caccgcgttc
      121 aggtcacggc acagggactc gaggaactct gggtggacta tcgagacggg aggttcgttg
      181 aatgtctgag gccgttgtcg tcggacacac tggcgcatcg ccttcgccgt gtgagcgctg
      241 attggccgga aggctatcga accgagatca acctccgagc atcggaatgg atgaagcacg
      301 ttgctcagca tctggatcgt ggattcgtgc tcaccatcga ttatggccat acggcacagg
      361 acctctatcg gtccgaccga aaacacggca cctttctttg ttacttcaga caatccatac
      421 gcgaagaccc gttccggcgc gtgggcgagc aggacatgac tgcccacgtg gatttttcga
      481 gcttagtgga gaccggtgaa gggcacaaac tccagacgac cggcttcacg aaccagatga
      541 gttttctcat gggactcggc gtcgaacaga tgatcgcgga attggaacag gacagtccgc
      601 agttcaaagc ggccattcat ctgctgagac cgaacggtat gggcacgacg ttcaaggtcc
      661 tcgtccaaca caagggaatc cagcagcctg aactcgacgg cttgcggtac aaaccgttct
      721 ttggttcggc gctcacaacg caatcagccg cgtgaggaat ttcgtatgac ggcatcatcg
      781 atgttcggca aggtgttctg aggtctgtat gggtgacaca gccgttccgg ataactatgt
      841 cccaatcctc atctttatcg gtgtgggtat tgcgctcggt acgttgactc tcttgcttgg
      901 aaaatttgtc cgtcccaatc agccctatcg ggcgaagtta gccccgtatg aaagcggaag
      961 cccgctcttt caggacgccc gcgtacagtt tccgatgcgg tattacatca tcgcgatgct
     1021 gttcgtgatc ttcgatatcg agattgtctt tatgtttccc tgggctgttg cattcaacag
     1081 tcttgggctg gttggattag tagagatggt tctattcatt gccatcctgg tggtggggtt
     1141 ttggtacgcg tggaaaaaag gagcactgga gtgggattga agggcgatgg gctatccttc
     1201 cgtaaaagag gtagataaaa gagcatgagt ctacttgagc gacagtttga cgcaaatatc
     1261 gtgacgacga atttggatgc cgtcgtcaac tgggccagga agtcggcatt gtggcccatg
     1321 accttcggat tggcttgctg cgccattgaa atgatcgcca gcgtctcctc ccgatatgac
     1381 ctggaccgct tcggcgccgg ggtctttcgc gcatccccca gacaatccga tctcatgatt
     1441 gtcgcgggaa cggtctcgcg caaaatggcg ccggtcattc gacggatcta cgaccaaatg
     1501 cctgaacctc gatatgtgat ctgcatgggt tcatgcgcga cttcgggcaa tcactacaac
     1561 agctatgcgg tcgtccaggg tgtggatcag atcgtaccgg ttgatgtcta tgtgcctgga
     1621 tgcccgccga gaccggaagc cttgctggac ggactgttga agttacagga aaagatccag
     1681 cgagagagag tgttcgtgaa gtagtcattg gtcattcgcc aacggtcatt acgctcgcca
     1741 tgtgccatct attgaccaat gaccattgac caggtatcaa tgccatgcaa tctctcattg
     1801 aaacgttact caacaagttc ccggaggcgg tgctctccgt cgatgtcgac acggcccgat
     1861 cggaagtcac c
//
LOCUS       DASTWE010001043         1306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1450198, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001043 DASTWE010000000
VERSION     DASTWE010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450198"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFT30_10855"
     CDS             <1..500
                     /locus_tag="VFT30_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5093179.1"
                     /translation="LDKLMPLVYSELRRLASNYLRRERQGHTLQPTALVNEAYLKLVD
                     QRNAKWQNRAQFFGISAQLMRRILVDHARHHQAAKRGGSDQQRLSITSAETVMKQPAV
                     DLLALNEALDELTKMDPQQGRIVELKFFGGLSIEETAEVLGIGHATVERDWKMARAWL
                     RRRLE"
     gene            561..>1306
                     /locus_tag="VFT30_10860"
     CDS             561..>1306
                     /locus_tag="VFT30_10860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5093180.1"
                     /translation="MKMTPERWQQVKAIFNSAIQHEPKDRASYLLRVCSGDTILLDEV
                     ESLIASHEKSGSFIDNPAYDRAPDFMEEHTELKKGEVVGSYEVLGLIGRGGMGEVYLA
                     QDQRLNRKVALKLLPTPSTQDIDRLSRFEHEARAASALNHPNILTIHEITEVNSVHLI
                     ATEYVEGETLRQRLNHSSLSLPEALDIAIQVADAVAAAHKAGIIHRDIKPENVMIRPD
                     GYVKVLDFGLAKLIESPGLTSDADDPTRRI"
BASE COUNT          334 a          326 c          361 g          285 t
ORIGIN      
        1 ccctggataa actaatgccc ctggtctata gcgagctccg acggctggct agcaattatt
       61 taagacggga acgccagggt catacgctgc aaccgacggc gctggtgaac gaagcctatc
      121 tgaagcttgt cgatcagagg aatgcaaaat ggcagaacag ggcccagttc tttggtattt
      181 cagcccagtt aatgaggcga attcttgtag atcatgcccg tcatcaccag gctgcgaagc
      241 gtggcggctc ggatcaacaa agactttcaa ttacgagtgc ggaaactgtg atgaagcagc
      301 ccgcagtcga tctgctggca ttgaatgaag ccctggatga gttaacaaaa atggacccgc
      361 agcaagggcg aatcgtcgaa ctcaagtttt tcgggggcct ctccatcgag gaaacggcgg
      421 aggtccttgg aattgggcac gccacggtgg aacgtgattg gaaaatggcc cgcgcctggc
      481 tgcgccggcg actcgaataa tcttgaggga ataaaaagac taactccgcg ttcgatatag
      541 agggattgtg tctctcgccg atgaaaatga ctccagaacg ttggcaacaa gtcaaggcga
      601 tttttaactc ggccattcaa cacgagccga aggaccgcgc ttcgtatctg ttgcgggtct
      661 gttccggaga caccatccta ctcgacgaag tggagtcgtt aatcgcgtcg catgaaaaaa
      721 gtgggtcatt cattgataac ccagcctatg accgtgcccc tgacttcatg gaagaacaca
      781 cggagcttaa gaagggcgag gtggtcggga gttacgaggt gctgggtcta attggccgcg
      841 gggggatggg ggaagtttat ctggcccagg atcagcgact gaaccggaag gtagcgttaa
      901 agttgctgcc tactccctca acacaagaca ttgatcgcct atctcgcttt gaacacgaag
      961 cgcgggcggc ttccgcttta aatcacccga acatccttac catacacgag atcacggaag
     1021 ttaattcagt tcatttgatc gctacggagt atgttgaagg agagacctta cgtcagcggt
     1081 tgaatcactc atcactctcg ctacccgaag cactcgacat cgccattcaa gtagctgacg
     1141 ccgtcgccgc cgctcacaag gctggaatca tccaccgtga tattaagccg gaaaatgtaa
     1201 tgattcgacc ggacggttat gtgaaggtcc tggattttgg tttggcaaaa ctcatcgaat
     1261 cgcccgggtt gacgtctgat gctgatgacc caacgcggcg aatcca
//
LOCUS       DASTWE010001044         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_577898, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001044 DASTWE010000000
VERSION     DASTWE010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577898"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            583..921
                     /locus_tag="VFT30_10865"
     CDS             583..921
                     /locus_tag="VFT30_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5093181.1"
                     /translation="MEIAAEDLTDGIAKVVLRGRFDTTGAVQVELPFNRIVTEQRKVL
                     VDLSAVSFLTSYAIRVLLIGAKIVNSKNGKLVILCPDGKVAKVLKTAGVDALIPVYVA
                     ESAAIEALRL"
BASE COUNT          239 a          297 c          313 g          241 t
ORIGIN      
        1 ctctcgatcg ggccgaaact gccttggcac acatggggac atgactgcta cttgccaatc
       61 ttgtgagcaa gtcctcctgc aggccggagt aaaggccaaa gccgcgggcc cgccttattc
      121 caagttggcc aatcgcccat cagcgtgcgg atcataggag gactgtgccc ggtcccccga
      181 gggaagtact taagtggccc tgaatcgttc tgattccatc ggtgagccgc tggcacgagc
      241 aagacaagtc aaatcggttc cttgagccgt gcaatcgctt ctcaccgcca tgggcctcct
      301 cgacgttgct gtagccttcg ggatttgggt aaagacgaag gtgtaacggt tctcgtcgtc
      361 ggcattgaca cagggaatgc ggaggcacct ctctggatgg ttcgattatc ggtcaggttc
      421 cgatcagcgc tgagaaatta ccttagcatg gagcggcatt atgatagaga tatgaatgga
      481 tctgaaacgt tctgaatcgg aatgctttcg gcgttccagc ctaaatcaaa caaggatatg
      541 acgggcctgc gttaccaccg ccgccataca cacaggtgat gcatggagat tgcggccgag
      601 gatcttacgg acggcattgc gaaggtcgtt ctgcgtggtc gcttcgacac gacaggcgcc
      661 gttcaggtcg agctgccgtt caatcgaatt gtaaccgaac aacgaaaggt cctcgttgac
      721 ctttcggccg tgagcttcct gacctcctat gccattcgcg tgctgctgat cggcgccaag
      781 atcgtcaaca gcaagaacgg caagctcgtg atcctgtgcc cggacggcaa ggttgccaag
      841 gtcctgaaga ctgccggtgt ggacgcatta attccagttt atgtcgctga gagtgccgca
      901 atcgaggccc taagactgtg acagcgatgt caggttcgga tccaagacgc ctagtgctag
      961 gcaaccacct tgccgagttg aaccgcctcg cagtatggat cgaggggtgg gcgcaggaca
     1021 gcgtatctcc tgacgtctcg ctcgctcttc aactgtgcct cgaagaggca gtcgccaata
     1081 tcatcatgta
//
LOCUS       DASTWE010001045         1810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_577905, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001045 DASTWE010000000
VERSION     DASTWE010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577905"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFT30_10870"
     CDS             complement(<1..713)
                     /locus_tag="VFT30_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093182.1"
                     /translation="MPEQTVARQDSRETLSFYAVGVLFLIVVPLLNVLPAEDSWLHVS
                     DFRLNQFGKFLAFAILALGLDLIWGYCGVLSLGQGVFFGFGAYCMGMYLSLQIGKESV
                     YGSELPDFMVWTQVKELPLFWYPFKSFIGGFLGAMLVPMLFATIFGFLAFRSRIKGVY
                     FAIITQALAFAAWLVFNRNETRLGGTNGLTDFKQLLGFRLSDPSTQRGLYILTVLALA
                     ASYMLCRWIVTSRAGNVLIA"
     gene            complement(706..>1810)
                     /gene="urtB"
                     /locus_tag="VFT30_10875"
     CDS             complement(706..>1810)
                     /gene="urtB"
                     /locus_tag="VFT30_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEU5093183.1"
                     /translation="LIQLRSEDPAVRMVAVKKLGELRSPNGLSVLKEFVETGAQSGAT
                     DEQRALAEAAQTSIGQIDSWSRWASVIETLFRGISLSSILLIMSLGLAIVFGLMGVIN
                     MAHGELMMIGAYATFVTQQAFIAWFSPEMFDWYFPVALPVAFVTSAAFGWVLEATVIR
                     FLYGRLLETLLATWGVSLILMQAARVYFGDLTAVIAPQALRGGAQVMVGVYLPYNRIF
                     IIMLSTICVLGIYFLLFRSDLGIRVRSVTQNRNMSACLGIPTRKVDSYTFAFASGLAG
                     IAGWALTMVGNVDPGLGQNYIVDSFMVVVTGGVGKLAGTIWASLGIGGLNKLIEPVSG
                     AVYGKVFILVGVILFLQWRPQGLFAAKGRSADA"
BASE COUNT          499 a          533 c          439 g          339 t
ORIGIN      
        1 gcgatgagta cgtttcccgc tcgcgatgtg acgatccagc gacaaagcat gtaggaggct
       61 gcaagggcca ggaccgtcag aatatacaac cctcgttggg tcgatggatc ggaaagccga
      121 aaaccaagca attgtttaaa atccgtcaat ccattggttc caccgagccg cgtctcgttc
      181 cgattgaaga ccagccaggc cgcaaatgcc aaagcctgcg tgatgatggc gaagtacaca
      241 cccttaattc tgctacggaa tgcaagaaac ccgaatatcg tcgcgaagag catcgggaca
      301 agcatcgctc ctaagaagcc gccaataaaa ctcttgaatg gataccaaaa gagaggcagc
      361 tccttgacct gagtccagac catgaagtca ggcaattcac ttccgtatac actttccttt
      421 ccgatctgca gcgacaaata catacccata cagtaggcgc caaagccgaa aaataccccc
      481 tgacctaaac tcaggacacc gcaatagccc cagatgagat ccagtccgag agccagaatg
      541 gcaaaggcca aaaacttgcc gaactgattg agacgaaagt ctgaaacgtg cagccaggaa
      601 tcttcagcag gcaacacatt cagcagtggg acgacgatca ggaacaagac cccgactgcg
      661 taaaaagaca acgtctcgcg gctgtcttgg cgagcgaccg tttgttcagg catcggcgct
      721 ccgtcctttg gctgcgaaca ggccctgcgg ccgccactgt aggaataaaa tcacacccac
      781 caagatgaag accttcccgt agaccgcccc gctcaccggc tcgatcagtt tgttcagccc
      841 gccgattcct aatgaagccc agattgtccc ggcaagcttt ccgactcccc cggtcaccac
      901 aaccatgaac gaatcgacga tgtagttctg cccgagtcct gggtcaacat ttcccaccat
      961 cgtgagcgcc catcccgcaa tgccggccaa tcccgatgca aaggcaaagg tataggagtc
     1021 gacctttctg gtcgggatac cgagacaggc gctcatattg cgattttgcg tgaccgatcg
     1081 tacccgtatc ccgaggtctg accggaagag caagaagtaa atccccagaa cacaaatggt
     1141 cgacagcatg atgataaaaa ttcggttata gggaagatag actcccacca tcacctgtgc
     1201 accacctcgc aaggcctgcg gggcgatcac cgcggtcaga tcgccgaagt atactcgggc
     1261 cgcctgcatc aggatcaaac tgactcccca tgtcgccaaa agagtttcta gcaatcttcc
     1321 gtaaagaaaa cggatcacgg tagcttcgag cacccatccg aatgcggcgg acgtcacaaa
     1381 ggccacgggc aatgccacag ggaagtacca gtcaaacatc tcaggagaaa accaggcgat
     1441 gaaagcctgt tgcgtgacga acgtggcata cgcgccgatc atcatcagct cgccatgcgc
     1501 catgttgatc acgcccatca aaccgaagac gatggccagc ccaagagaca tgatcaagag
     1561 aatagaactg agactgattc cccggaatag tgtctcaatg acgctcgccc accgactcca
     1621 ggagtcaatt tgtccgatcg aagtttgggc cgcttccgcc aacgctctct gctcatcggt
     1681 agccccactt tgggcacctg tttcaacaaa ttctttgagc actgacagcc cgttcggact
     1741 tcggagttcg ccgagcttct tgacggcaac catccgaaca gctggatctt cagaacgaag
     1801 ttgaatcagt
//
LOCUS       DASTWE010001046         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1514807, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001046 DASTWE010000000
VERSION     DASTWE010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514807"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VFT30_10880"
     CDS             complement(<1..522)
                     /locus_tag="VFT30_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5093184.1"
                     /translation="MTAPSPLILPTKAHAIAHDLRALLGTEKVKDDAPTITAYAVDAS
                     IYRIPPQAVVLVESEEDIAATVAYAVTRGIPLTPRAAGTNLTGSAIGSGIILDASRLN
                     RILEVNREERWARVQPGIVLAELNKQLSLQGLLFGPDPSSGDMCKLGGMVANNSSGPH
                     TLRYGSVKDNVQSL"
     gene            610..>1241
                     /locus_tag="VFT30_10885"
     CDS             610..>1241
                     /locus_tag="VFT30_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5093185.1"
                     /translation="MDRPTLYITRLLPRPVLDAISGQYRMLAEPTDRPPTVEELRHGF
                     AEAQAVICTLTDRIDASLLSHATKLKIIANYAVGYNNIDLPAAAQRGIIVSNTPDVLT
                     ETTADLTWALLLALTRRVVEGDAWVRTGHWPGWTPTQMLGADVSGKTLGIIGMGRIGQ
                     AVAQRAAGFRMPVIYTGRHGVSNPHGVSWIRRSLDDVLGESDFLSLHVPLT"
BASE COUNT          255 a          359 c          356 g          271 t
ORIGIN      
        1 gagactctgg acattgtctt ttaccgaccc gtagcgaaga gtgtgcggtc ccgaggaatt
       61 attggcgacc atgccgccca atttgcacat gtccccactc gatggatccg gcccaaacaa
      121 cagcccctgc aagctcagtt gtttgttcaa ttcggccagt actattcccg gctggacccg
      181 agcccatctc tcttcccgat taacttcaag gattcgattc aggcgcgatg cgtcgagaat
      241 gatgcctgag ccgatggcag aaccggtcag gtttgtcccg gctgcccgag gcgtaagagg
      301 aatgccgcgt gtcaccgcat aggcgaccgt tgcagcgatg tcctcttctg attcaacaag
      361 gacgaccgcc tgtgggggaa tgcgatagat gctggcatcg acggcgtagg ccgtgatcgt
      421 gggcgcgtcg tctttgactt tctctgttcc caggagagcg cgaaggtcat gagcgattgc
      481 gtgagctttc gtcggcaaga tcaatgggga tggagccgtc atagataatg atccatctta
      541 gctcatgaac tctcagaagc accagggtcg ttgtcggtca cctacgaatt ctcctacaat
      601 ggaaagccca tggaccgtcc gactttatat atcacccgac tgttgcctcg accggtgctg
      661 gacgcaatct ccggacagta tcggatgctg gccgagccga cagatcggcc accgacggtt
      721 gaggagttgc gccatggttt cgccgaagct caggcggtga tctgcaccct gacggatcgt
      781 atcgatgctt cgttactgtc ccacgccacg aaattgaaaa tcatcgcgaa ctacgcagta
      841 ggctacaaca acatcgatct tcctgccgcg gctcaacgcg gcatcatcgt gtccaataca
      901 ccggacgtgc tgacagagac caccgctgat ctgacctggg cgctattgct cgccctgacc
      961 agacgagtcg ttgaaggaga tgcgtgggtt cgaaccggtc actggcccgg atggactccg
     1021 acacagatgc tgggcgccga cgtgtcaggg aagacgctgg ggatcatcgg catggggcgg
     1081 atcggacagg ctgtcgcgca acgggcggcc ggatttcgga tgcccgtgat ctatacaggc
     1141 cgccacggcg tttccaaccc ccacggtgtc tcatggatac ggcgcagcct cgatgatgta
     1201 ctcggtgagt ctgattttct ttcgctccat gtccctctca c
//
LOCUS       DASTWE010001047         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_868288, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001047 DASTWE010000000
VERSION     DASTWE010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868288"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFT30_10890"
     CDS             <1..456
                     /locus_tag="VFT30_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU5093186.1"
                     /translation="VAISQEGLDTFRFDVGATYAVRVVRLVLAHGLPEETLVNVNIPN
                     RSRREIKGVRVTCLSRRRFHNPIIEKLDPHGRKYYWIAGERISWSRSKDADHEAIEEG
                     FVSITPIHLDMTHYGVLDRFRAWEPIMNRGIKKSSTARSTIGRTRKLRA"
     gene            372..1094
                     /locus_tag="VFT30_10895"
     CDS             372..1094
                     /locus_tag="VFT30_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU5093187.1"
                     /translation="MGADHEPGHQEILNRSVYDRPYKEVKGMMGGLIEAIISELSRFV
                     IACISRFGYGGILFTMAVESACIPLPSEIIMPFSGYLVMTGQFTMLGVTLAGAVGNVL
                     GSIVAYYAGVWGGRPFAERYGPYFLVSHHDLDIADRWFEKYGEAAVFFSRMLPVVRTF
                     ISLPAGIAKMNFPRFVVFTFVGALPWCYLLAYIGLRMGEQWEHLRDYFHQFDIVIGLV
                     LAAAVGYFLWSHWPKRRASPGA"
     gene            1100..>1853
                     /gene="cysS"
                     /locus_tag="VFT30_10900"
     CDS             1100..>1853
                     /gene="cysS"
                     /locus_tag="VFT30_10900"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5093188.1"
                     /translation="MLRLFNTLTERQEIFEPIEPKKVRMYVCGVTVYDYCHIGHARSA
                     LVFDVLRRYLEYSGYAVTFVKNFTDVDDKIIKRANEQGITCGAVTAKYIQAYHEDMGK
                     LGIRAATGEPKATEHIDAIIQLTEQLVHKGLAYVVDGDVYFEVAKYPEYGRLSKRRLE
                     DLQAGARVDVDERKRHPIDFALWKNSKPGEPAWESPWGLGRPGWHIECSAMSIRHLGE
                     TFDIHGGGMDLIFPQHENEIAQSCGATGKEFAL"
BASE COUNT          420 a          448 c          556 g          429 t
ORIGIN      
        1 gtggcgatat cccaagaagg gctggacaca tttcgcttcg acgttggcgc aacctatgcg
       61 gtacgcgtgg tgcggcttgt ccttgctcac ggtctgcccg aggagacgct cgtgaacgtg
      121 aatattccaa atcggtcgcg acgggaaata aagggcgtgc gggtcacctg tctcagccga
      181 aggcggtttc ataatccgat catcgaaaag ctggacccgc acggacggaa atattattgg
      241 attgccggcg agcggatatc gtggagtcgc agtaaggacg ctgatcatga ggcgatcgaa
      301 gaagggtttg tctccatcac gccgatccat ctggatatga cgcattatgg ggttctggat
      361 cgcttccgcg cgtgggagcc gatcatgaac cggggcatca agaaatcctc aaccgctcgg
      421 tctacgatcg gccgtacaag gaagttaagg gcatgatggg aggactgatc gaagccatca
      481 tcagtgagtt gagccgattc gtcattgcgt gtatttcaag gttcggctac gggggcatcc
      541 tctttacgat ggctgtcgaa agcgcctgca ttccgctccc gagtgaaatt atcatgccgt
      601 tttccggcta tctggtcatg actggacagt ttacgatgct cggggtcacg ctggccggcg
      661 cggtcggcaa cgttctcggt tcgatcgtgg cgtactacgc gggcgtctgg ggcggccgac
      721 catttgcaga gcgctatgga ccgtattttc tggtttcgca ccacgatctg gatatcgccg
      781 atcgctggtt cgaaaaatat ggagaggctg cagtcttttt cagcagaatg ctcccggtgg
      841 tgcgcacgtt catttcgctc ccggccggca ttgcgaagat gaatttcccg cgatttgtcg
      901 tcttcacctt tgtaggggca ctaccttggt gctatctgtt ggcgtatatc ggccttcgta
      961 tgggcgaaca gtgggagcat cttcgggatt actttcacca atttgacatc gtcatcggac
     1021 tggtcctagc tgcagccgtt gggtacttcc tttggtctca ctggccaaaa cgacgagcga
     1081 gcccaggagc ttaaacggga tgcttagact gttcaatacg ctgactgagc ggcaagaaat
     1141 tttcgaaccg attgaaccga agaaggtacg catgtacgtc tgtggcgtga cggtctatga
     1201 ctattgccac atcggtcatg ctcgcagtgc gttggtattc gacgtgctcc gtcggtattt
     1261 ggaatacagt ggctatgcgg taaccttcgt caagaacttc acggatgtgg acgacaagat
     1321 catcaaacgg gcgaatgaac aggggatcac ttgtggtgcc gtgacggcca agtacattca
     1381 ggcctaccat gaggatatgg gcaagctggg aatccgagct gcgacgggag aacccaaggc
     1441 cacagagcac atagatgcca tcattcagct cacggaacaa ttggtccaca aagggctggc
     1501 gtacgtggtg gatggagatg tgtattttga agtcgcgaaa tatccggaat acggaaggct
     1561 gtcaaaacga cgactcgagg acttgcaggc cggtgcgcgg gtagacgtag atgaacgaaa
     1621 gcgtcacccg atagattttg ccctgtggaa gaacagtaag ccaggtgaac cggcatggga
     1681 aagcccatgg ggattaggac gaccaggctg gcatatcgag tgttccgcta tgtcgatccg
     1741 gcacctcggc gaaactttcg atatccatgg cggtgggatg gatttgattt tcccgcaaca
     1801 tgaaaatgaa atcgcacagt cctgcggagc gacgggaaaa gaatttgcac tct
//
LOCUS       DASTWE010001048         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_383256, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001048 DASTWE010000000
VERSION     DASTWE010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383256"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFT30_10905"
     CDS             <1..325
                     /locus_tag="VFT30_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU5093189.1"
                     /translation="PTGSGWWHWVIFNIPPDVTALAADAGKPDGPGAPQLSMQSMTDF
                     GQPGYGGPCPPPGHKPHRYIFTVFALKVDQLPLKSEASGAMVGYYLNQNALAKASLTG
                     LYSRK"
     gene            348..806
                     /locus_tag="VFT30_10910"
     CDS             348..806
                     /locus_tag="VFT30_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093190.1"
                     /translation="MPLDAELGKKMLQLITSRYDDRHWRKQIEKTLSLPQSGVADPIQ
                     QQIFMYLKHGLKAYKSRRSDPDSWIIGGYATKEVINRAKFQTQLVGSSITKDDVAFLG
                     TDPGDDVTQAWWEEMLVQWFDVPEEEKPAEDKEGGTAADEDSSATFKTKA"
BASE COUNT          257 a          281 c          282 g          222 t
ORIGIN      
        1 tccgaccggc agcggctggt ggcactgggt catcttcaat attcctcccg atgttacggc
       61 gctcgccgcc gatgcgggaa agcctgatgg cccaggcgct ccgcaactca gcatgcagag
      121 catgacggat tttggtcagc ctgggtacgg tggaccctgt cctcctcctg gacacaagcc
      181 gcatcgctac atctttacgg tgtttgcatt gaaagtggac caattaccat tgaaatccga
      241 ggcatctgga gcgatggtcg ggtattatct caaccagaat gccctggcga aggcctcatt
      301 gaccggccta tacagccgca agtaacgacg agtgagaacc tcacaccatg ccgcttgatg
      361 cagagttagg aaagaaaatg cttcagctga tcacgtcgcg ctacgatgat cggcattggc
      421 ggaagcaaat cgagaaaacc cttagtctgc cccagtccgg tgttgccgat ccaatccagc
      481 agcagatctt catgtacctc aagcacggcc tgaaggcgta caagtcccgc cgatccgatc
      541 cggactcctg gattatcggt ggctatgcca cgaaagaagt catcaatcga gcgaaattcc
      601 agacgcagct cgtcgggtca tctatcacga aagacgacgt ggccttcctg gggaccgatc
      661 caggagacga tgtgacccag gcctggtggg aagagatgtt ggtccagtgg ttcgacgtgc
      721 cggaagaaga gaaacctgcc gaagataaag aaggtggcac agctgccgac gaggactcct
      781 cagcaacgtt caagacgaaa gcataacgat cgttcggcgc tcaaggtttt gtgacggacg
      841 aaagcttttg tatagcatct gtcagccgat ctttcgcgcc attgaaaaac ctcacagctt
      901 tctatcctct ccacctctgt tggaggcggg cctgaccgtt tccgcgagca ggagtagtta
      961 gctggccaaa atctgtcaag tgcgatcatg aagttgtccc ttttgagttt ggcaagtaca
     1021 tgaccaaaat gaaatacaac tg
//
LOCUS       DASTWE010001049         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1546981, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001049 DASTWE010000000
VERSION     DASTWE010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546981"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFT30_10915"
     CDS             <1..445
                     /locus_tag="VFT30_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093191.1"
                     /translation="SLENSALLNAAYRTLKDPIQRAEYLVRLEAGAVKEIRNQPPADL
                     FEEILSLQDDLEAFRAAHGQPSADLTALRTKLQTDREALERRQQDMESRLFDLFETWD
                     RLQRLSEPTEAIRAKKESVLKEMRELLSNRTYVRNIINDLIATVG"
     gene            454..>1083
                     /locus_tag="VFT30_10920"
     CDS             454..>1083
                     /locus_tag="VFT30_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU5093192.1"
                     /translation="MARIVGIDLGTTNSLIAYMDGKTPTVVPGRNGRTMVPSVVAMTD
                     NGLIVGDAAKEHLVRNPERTIYSVKRFMGKGLADVRSEVPYFPYTLQEQGGVIRIRIG
                     DKSYSPPQISAMILKELKHRAEAHLGESIAKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQERTQGTIAVYDLGGGTFDISILKLKNGIFEVL"
BASE COUNT          286 a          302 c          269 g          226 t
ORIGIN      
        1 cagcctggag aactctgccc ttctgaatgc ggcatatcga acgctcaagg atcccattca
       61 acgagccgaa tatctggtgc gattggaagc aggggcagtc aaagagattc gtaatcaacc
      121 gcctgcagat ctattcgaag aaattctgtc tcttcaagat gatctggaag cgtttcgcgc
      181 agcgcacgga caaccatcgg cggatctcac ggcattacgc accaagctac aaacggatcg
      241 tgaagctctt gaacggcgtc aacaggatat ggagtcccga ctcttcgacc tctttgaaac
      301 ctgggaccgt ttgcagcgcc tatccgaacc cacggaggcg attcgcgcaa aaaaggaatc
      361 cgtcctcaaa gagatgcgcg aactactttc caaccgaacc tatgtccgca acatcatcaa
      421 cgacctcatc gcaaccgttg gataacaaca cccatggctc gtatcgtcgg catcgatctc
      481 ggcaccacga attcacttat tgcctatatg gacgggaaaa ctcccaccgt ggttcctgga
      541 cgaaatggtc gcaccatggt gccatccgtc gtcgccatga ccgacaatgg actcattgtg
      601 ggcgatgccg cgaaagagca tttggtacga aacccggaac ggaccatcta ttcggtcaaa
      661 cgtttcatgg ggaaaggcct ggccgacgtt cgaagcgagg tcccctattt cccctatacc
      721 ctgcaagaac aaggcggggt gatacgcatt cgcatcggtg acaaaagtta ttctccgccc
      781 cagatatccg cgatgattct caaagaactc aaacatcggg cggaagccca tctgggagaa
      841 agtattgcga aggctgtcat cacggtgccg gcctatttca acgatagtca gcgtcaggca
      901 accaaagacg ccggtatgat cgcgggatta gaagtactgc gaatcatcaa tgaaccgacc
      961 gccgcttcgc tagcctatgg cctccaagaa cggactcaag gcaccatcgc agtatacgat
     1021 ctcggcggtg ggaccttcga catttccatt ttgaagctga aaaacggcat ttttgaagtg
     1081 ttg
//
LOCUS       DASTWE010001050         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1288304, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001050 DASTWE010000000
VERSION     DASTWE010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288304"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VFT30_10925"
     CDS             <1..1441
                     /locus_tag="VFT30_10925"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEU5093193.1"
                     /translation="LPIALANLGYTDQQTQDIVRYCVGAQTLKGAPFINHDTLRQKGF
                     DDAALGRVESSLGQAFEIQFVFNKFTLGDAFCVEKLGLTEAQLNETNFNMLKTLGFTQ
                     EEIAAANDFCCGTMTVEGASHLKAEHLAIFDCANRCGRIGQRSIAVDAHIRMMAAAQP
                     FISGAISKTINMPADATLEDVKAAYLLAWKSMVKAVALYRDGSKLSQPLSASTDSGKA
                     VEAVTGVMELAEKVTERVLVRYLAKRRPLPSRRSGYTQKAIVGGHKLYLRTGEYEDGT
                     VGEIFLDMHKEGAAFRSLMNCFAIAISLGLQHGVPLEEFVEAFVFTRFEPNGPVKLND
                     RIKMSTSIIDYIFRELAVTYLDRYDLAQVKEEDLRMDSVKKDHMDPECFDEEADLDAL
                     AKSSVITEHLPIRRNGGKGNGHGNGHGVARQVEIMRETLTLTEVQNAKEAKVKGYEGD
                     PCQECKQFTMVRNGTCLKCVTCGATSGCS"
BASE COUNT          374 a          381 c          443 g          313 t
ORIGIN      
        1 cttgccgatc gcgctggcca acctcggcta tacagatcag caaacgcagg atatcgtccg
       61 gtattgtgtc ggtgctcaga ctctcaaggg tgcaccgttc atcaatcacg acacgctgcg
      121 tcaaaaggga ttcgacgacg cagccctggg tcgtgtagag agtagtttgg ggcaagcgtt
      181 cgaaattcag ttcgtcttca ataagtttac gctaggagat gcgttctgcg tcgaaaagct
      241 tggtttgacc gaggcccaac tgaatgaaac gaacttcaat atgctgaaaa cgctcggttt
      301 tacgcaagaa gagatcgctg ccgcaaacga tttctgctgc gggacgatga cggtggaagg
      361 cgcgtcacat ttgaaggccg aacacctcgc gatattcgac tgtgccaatc gatgcggtcg
      421 aatcgggcaa cgctccatcg ccgtcgatgc gcatatccgc atgatggccg cggcacaacc
      481 gtttatcagc ggcgcgatca gcaagaccat taacatgccg gccgatgcca cgctcgaaga
      541 cgtcaaggcg gcctatttgc ttgcttggaa gagcatggtc aaagcggtcg cgctgtaccg
      601 tgacggctcc aagctgagtc aaccattgag tgcttcgacg gacagtggaa aggctgtgga
      661 ggcggtcacc ggtgtgatgg agctggcgga gaaagtcacc gaacgggtgc tcgttcggta
      721 tctcgccaag cggcgtccgc taccaagtcg acgaagcgga tacactcaaa aggccattgt
      781 cggaggacat aagctctacc tgcgcaccgg cgaatacgag gatggaacgg tcggggaaat
      841 ctttttggac atgcacaagg agggggcggc gttcagaagc ctcatgaatt gttttgcgat
      901 cgccatctct cttggacttc agcacggcgt gccgctggaa gaattcgtcg aggcgtttgt
      961 ctttacccgg tttgagccga acggtcccgt caagttgaac gatcgaatca aaatgtccac
     1021 ctcgatcatc gattatatct tccgtgaatt ggccgttacg tatctcgatc gatacgacct
     1081 cgcgcaggtg aaagaagaag atctacgcat ggattctgtg aagaaagacc atatggatcc
     1141 cgaatgcttc gatgaagaag cggacctgga tgccctggct aaatcgtccg ttattacaga
     1201 acatcttccc atccgccgaa acggcggaaa aggaaatggc cacggaaacg gacatggtgt
     1261 tgcccgacaa gtggagatca tgcgtgaaac gctcacactg acggaggtcc aaaacgctaa
     1321 agaggccaag gtcaagggct acgaaggcga cccctgccag gaatgcaagc aattcaccat
     1381 ggttcgcaac ggcacctgtc tcaaatgtgt gacgtgcggc gcgacgagtg gatgttcata
     1441 agaggagtat tgaggatgca gagcaagcgg ccgtcatcgt gcctctggtg taggaagcgg
     1501 cggttcgtac g
//
LOCUS       DASTWE010001051         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1288340, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001051 DASTWE010000000
VERSION     DASTWE010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288340"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFT30_10930"
     CDS             complement(<1..1163)
                     /locus_tag="VFT30_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5093194.1"
                     /translation="MQERKPRFTSLSGFDTNRVYTHNDLKDWSPDEELGAPGQFPYTR
                     GVYPTMYRSRFWTMRQFAGFGSAEDTNRRFKYLLQHGQNGLSVAFDMPTLMGIDADAP
                     LAHGEIGHCGVSISSLDDMERLFDGIPLDQVTTSMTINGPAAVIFAMYLAVAEKRGIR
                     LERLDGTLQNDILKEYIAQKEWLYPPEPSLRLITDTIAYCSEHVPKWHPVSVSGYHIR
                     EAGSTAVQELAFTLYDGLTYVEAAIKAGLPVDRFAPQLSFFFNAHNDFFEEIAKFRAA
                     RRLWAREMTRRYRPGDPRSVQLRCHAQTAGCSLTAQQPMNNVVRTTIQALAAVLGGAQ
                     SLHTNSMDETLALPTEDAVKLALRTQQIIAHESEVTNSVDPLGGSYYVETLTNR"
     gene            complement(1203..>1646)
                     /locus_tag="VFT30_10935"
     CDS             complement(1203..>1646)
                     /locus_tag="VFT30_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5093195.1"
                     /translation="LVEVVRGLETSERTTQLALDLAKRLGKTPVVAKDVPGFIVNRVL
                     IPMINEAIFALEEGVASAEDIDLAMTAGANHPMGPLALADRIGLDTVLAICDVLHQDL
                     GDPKFRACPLLRRYVEAGWLGRKRGRGFHVYEEKNERQEAIGSTS"
BASE COUNT          350 a          473 c          475 g          348 t
ORIGIN      
        1 cgattcgtga gggtttcgac atagtaggag ccgccgagag gatcgacgct gttggtgact
       61 tcgctctcat gtgcaatgat ctgttgcgtc cgcaatgcca gtttcaccgc atcttcggtc
      121 ggcagcgcga gcgtttcgtc catcgaattg gtatggagcg attgcgcacc tcccaagacg
      181 gcggccaggg cctggatggt cgtgcggact acattgttca tgggctgttg tgccgtcagg
      241 gaacagccgg cagtctgcgc gtggcagcgg agctgcaccg agcgaggatc gccggggcgg
      301 taccgccgcg tcatttcgcg ggcccatagt cgacgggccg cgcggaactt ggcgatctcc
      361 tcaaaaaagt cattgtgagc gttgaagaaa aacgagagtt gcggagcgaa tcggtccacg
      421 ggaagaccgg ctttgatcgc cgcttccaca tacgtcaggc cgtcgtacaa ggtaaaggcc
      481 agttcttgga ccgcggtcga accggcttcc cggatgtgat atccgctgac actgaccgga
      541 tgccatttgg ggacatgctc ggaacagtac gcgatggtat cggtaatcaa acggagggag
      601 ggttccggcg gatagagcca ctctttttgc gcgatatatt ccttcagaat gtcgttctgc
      661 aaagtgccgt ccaatcgctc aagacgaatg cccctcttct ccgcaaccgc gaggtacatc
      721 gcaaagatca cggcagccgg accgttgatc gtcatcgagg tggtgacctg atcaagcgga
      781 ataccgtcga acagtcgctc catatcgtcg agcgacgata tcgagacgcc gcagtggccg
      841 atctcgccat gagcaagagg ggcatccgcg tcgatcccca tcaacgtggg catatcaaac
      901 gcgacgctca ggccgttttg cccatgttgg aggagatatt tgaagcgacg gttggtgtcc
      961 tccgccgaac cgaagccagc aaactgtcgc atggtccaga acctactacg atacatagtc
     1021 ggatagaccc ctcgcgtgta cggaaattgg cctggcgcgc caagttcctc gtccggcgac
     1081 cagtctttca aatcgttgtg ggtatacacc cggttggtgt cgaatcctga gagggaggtg
     1141 aatctcggtt tacgttcttg catcgatctg ctccagaccg tgcggagaaa ccaggaccaa
     1201 gatcaggacg tactgcctat cgcttcctgc cgttcatttt tctcttcgta gacatggaat
     1261 ccgcggccgc gcttccgtcc caaccatccc gcttcgacat accggcggag taaaggacaa
     1321 gcgcgaaatt tgggatcgcc caagtcttga tgcaagacat cgcagatggc caacactgta
     1381 tccaaaccga tgcggtcggc cagggccagt ggtcccatgg gatgattggc acccgcagtc
     1441 atcgccagat cgatgtcctc cgcagaggca accccttcct ccaatgcgaa aatcgcttca
     1501 ttgatcatgg gaatcagcac gcggttcacg atgaaccccg gtacatcctt agccacgacc
     1561 ggcgttttgc ccaagcgctt tgccaagtcg agtgcgagct gcgtcgttcg ttcggatgtt
     1621 tccagcccgc gaaccacttc caccag
//
LOCUS       DASTWE010001052         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1514924, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001052 DASTWE010000000
VERSION     DASTWE010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514924"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1246
                     /locus_tag="VFT30_10940"
                     /pseudo
     CDS             11..1246
                     /locus_tag="VFT30_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
     gene            1389..>2077
                     /locus_tag="VFT30_10945"
     CDS             1389..>2077
                     /locus_tag="VFT30_10945"
                     /inference="COORDINATES: protein motif:HMM:NF023301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5093196.1"
                     /translation="MKYTQNYMSRTIVDFDNGSIVVETLDDKSPKESLKNAIITTLLT
                     PDDPRSVDLFSDKPVTLTSAHPPYLLSLVLDQQGQPIRTPAQAEAFAASILDSTKTRT
                     VDQNGAIKQALIAEIKMVANFSNRQADKYRATVTRFDEQFKISPSLIFAVIRTESNFN
                     PFAVSSAPAYGLMQLVPTSGGRDAYRRAKGKDTTPSRDYLFDPENNIELGSAYLNVLS
                     YNLLEQIDNEVS"
BASE COUNT          565 a          575 c          535 g          402 t
ORIGIN      
        1 ggccgaggcg atggaagact cctcggccgc aggattggat gaaaacgaca gcgcaatcgt
       61 ccgtctggcc aaccaaatca ttgccgatgc ctatcgccaa aatgcatcgg atattcacat
      121 cgagccctac ggagaaaaac gtgaaactct ggtccgtttc cgagtcgacg gtgactgttt
      181 cgagtacatg aagatcccgc agagttaccg tcgtgctatc gtctcccgac tcaagatcat
      241 ggccagcctg gacattgccg aacgccgcaa acctcaagat gggaagatca aattcaagct
      301 ctcggagacg aaagagatcg aacttcgcgt cgcgactctc cccacggccg gatacaacga
      361 agacgtggtc atgcgtatcc tcgcggcgag tgaacctctg cctctcgaca agatggggtt
      421 ctcggaacgg aacctctaag tgctcaagga gatttcagaa aaaccctatg gcatcatact
      481 ttgtgtcggg ccgaccggat ccggaaaaac gaccacgctg cattcagtcc tcggcaacat
      541 taacactccc gatataaaaa tatggacggc agaagatcct gtcgaaatta cacagtatgg
      601 cctgcgtcaa gtacaggtcc agcccaaaat cggtctgaca ttcgctaacg ccatgcgtgc
      661 atttctccgg gccgaccccg atgtcatcat ggtgggagaa atgcgggata aagagacggc
      721 cgacaccggc atcgaagcgt cgctcaccgg ccatctggta ttgagcacac tgcacaccaa
      781 cagcgcagtc gaaacggtca cccgcctgct cgacatgggt tgcgatccgt tcagtttcgc
      841 cgatgccatg cttggcgtgc tcgcacaacg attagctcgc cggatctgca aggactgcaa
      901 ggagcagtac atcggaagca aagaggagta tgaagaactt cgattggggt atggacctga
      961 gtattgggat aagctcggca tcaaacagga caatacattc aggttgtccc gtgggaaggg
     1021 atgcgaaaca tgcaaccggt ccggctttag gggccgggta gccctacacg aactcctctt
     1081 gggaacggac caaataaagc gaatgattca acaaaaggcg cgtaccgaag agatgctgaa
     1141 aatcgcgctt gaagaaggga tgacgacgct cgtgcaagac ggcgttcaaa aagtcttgca
     1201 aggccacacg acatacaaag aagtcaaagc cgtcgccatc aaatagctct gtcgacttat
     1261 cccctaccgt ctttcgggag tcaggctctt tcactcctta cccacaaccg tgtagaattc
     1321 gccgcccaat ccgggtccga caaacctggg gcgagaaaga agtgaaggtc ccagagcaaa
     1381 aaaggtacgt gaaatacacc cagaactaca tgagccggac tatcgtggac ttcgacaacg
     1441 ggtcgatcgt ggtagaaaca ttggacgaca aatcgccgaa ggaaagcctt aagaacgcga
     1501 tcatcacgac cttgctcaca cctgatgatc cacgctccgt cgatcttttt tccgacaagc
     1561 cggtcacgct aaccagtgcc caccctccat acctattgag tctggtgctg gatcaacaag
     1621 gacagccgat ccgcacccct gcccaagcgg aagcgtttgc agcatcgata ctcgatagca
     1681 ctaagacgag aacggtggac cagaacgggg cgatcaagca agcgctgatc gctgaaatca
     1741 agatggtcgc gaatttctcg aatcgtcaag cagataagta tcgggcgacc gtcaccagat
     1801 tcgacgagca attcaagatc agcccaagcc tgatctttgc cgtcattaga acggaaagca
     1861 acttcaaccc ttttgccgtg agttccgctc cggcctatgg cctgatgcag cttgttccca
     1921 ccagcggagg ccgagacgcc tatcgaagag ccaaggggaa agatacgact ccatcgcgcg
     1981 attacctgtt cgacccggag aataacatcg aactcggcag tgcctatctc aatgtcctgt
     2041 cgtataattt gctcgagcag atcgacaatg aggtttc
//
LOCUS       DASTWE010001053         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_642196, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001053 DASTWE010000000
VERSION     DASTWE010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642196"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="VFT30_10950"
     CDS             <1..1940
                     /locus_tag="VFT30_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEU5093197.1"
                     /translation="SVATIPPESQQPYTLLAYSNEIDRIILRHGNDAVSVWQLGKKLI
                     PLKHIALDQKKEVSSVAASPDTKLLAIAQGHTVHLHDLSGTVIRDVAIAGGKPRDLLF
                     APNGSTLAASVGHTILFIDTETASVSAHGMLTSAEGAQGSLTPEVFSRDGHRLVAANG
                     EWSYGLFNTDTGELVTLTEFTYAAPGRGMRSPTQLRAVDIAADADLLVGQPEHLHTLQ
                     IWDLQTGAMLPDLCGEDCRNMSPRVSLLKWSPTGSKIVVGMQGGLNPDVDGKISVWDI
                     SSRSPELVLDPSQPQAKVLAKRMTLPTFITTTPSSPAAPAFVHARALRAVAMSPSANL
                     LVTTGDDGLLKIWDPGQGVLLRQLALSAPAHAIAFSADGAILAAGTVEGGVRLWETQT
                     WREFSPYSSRQGRINALQFLPGNRFLALAGGRSKVLVVDLVTRTLVNELVHTSPSPAC
                     GEGSCTKRRAVRGEVIDSLSFLDGSSFLLTTSPSGPVVWDITTWREVEKPAGLPDAWS
                     GLGWKHSFVWTTIRSAVPTAFTLAVWDTKNNKVWASLDTFTNRDTQIDHGPAVSLGTS
                     MAVDPLHRWAATRVGEHISVWDLSAQAKQKTFHVKTPYHLHWTSDGKYLIVATLDRKI
                     LVWSAETMEPVHYLRDPSVTR"
     gene            2057..>3765
                     /locus_tag="VFT30_10955"
     CDS             2057..>3765
                     /locus_tag="VFT30_10955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEU5093198.1"
                     /translation="MVKKKESASDTGSQSSAPRANPEPAVTKKVSAKPAERVTAVEMG
                     ARQREISVSEFFTKNRHLLGFDNPRKALLTCVKEAVDNALDACEEAGILPDVTVRLEV
                     VSNGEPVAPSQATRFRVTVTDNGPGIVRQQIPRIFAKLLYGSKFHRLRMSRGQQGIGI
                     SAAGMYGQLTTGKPVKIISRIGPKAAAHFFEVQIDTKKNEPLVHENKQIEWEQPRGTQ
                     VTLEVEGKYQKGRASVDEWLEQTSIANPHVKLMYHTPEGEVKEYPRTYHELPPQPREI
                     KPHPYGIEFGMLLKMLQDTKSHTLSGFLAGDFCRVSPQMGDEICKAAKVSPTSKPRDL
                     KGPVAETLYKTLQETKIMAPPTNCISPIGEKAILSGLYKQIKGEFYTAVSRPPAVYRG
                     NPFIIEAGLAYGNRPQDQNKPQQPAMPKAEGEHEEDDSELARVIRYANRVPLLYQQSA
                     CSTFKAVLSTTWKNYGLTQSRGALPGGPMVIFVHMASVWVPFTSESKEAIADYDEIQK
                     EITLALRECGRRLGLFVRRRERAASEYRRRNIFELYIEEVVDACNRLKGGKLPKEKLK
                     EQL"
BASE COUNT          851 a         1036 c         1088 g          790 t
ORIGIN      
        1 tctcagtggc caccattcca ccggaatcgc agcagcccta taccttgctt gcctattcga
       61 atgagatcga tcggataatt ctccgtcacg ggaacgacgc tgtgtcggtg tggcagctag
      121 ggaaaaagtt gataccgctc aaacacatag cgctggatca gaagaaggaa gtttcttcgg
      181 tggctgcctc gcccgatacg aagctgttgg cgattgcgca aggtcatacc gttcacctgc
      241 acgacctcag cgggacagtc atccgtgacg tggctatagc aggtggcaaa ccgcgtgatc
      301 tcctctttgc gccgaacggg tccactctcg cggcaagcgt cggccacacg attctcttca
      361 ttgatacgga aacagcatcg gtatccgcgc acggcatgtt gacgagtgct gaaggagcac
      421 agggctctct cactcccgag gtcttctcgc gggacggtca tcgcttggtg gcagccaacg
      481 gggaatggag ctatgggctt ttcaatactg atacgggcga gctggtaacc ttgaccgagt
      541 tcacctatgc agctccaggg cgcgggatgc gctcaccgac acagcttcgt gccgtcgaca
      601 ttgctgcgga tgcagatctc ttggtcgggc aaccggagca tcttcacacg ttacagatct
      661 gggatctaca aactggtgcg atgttgccgg acctgtgcgg agaggattgc cgcaacatga
      721 gcccccgagt ttcgttgctc aaatggtctc cgaccggttc gaagattgtg gttggaatgc
      781 agggtggcct caatcctgat gtggatggaa agatatcggt ctgggatatt tcctcacgtt
      841 ccccagagtt ggtattggat cccagtcaac cccaggccaa agtattggcg aaacgaatga
      901 ccctaccgac atttattacg acgacacctt cttccccagc tgcgccggct ttcgtccatg
      961 cacgggcctt gcgcgcagta gcgatgtctc ccagtgcgaa tctgctcgtc acgaccggcg
     1021 acgatgggct gctgaaaata tgggacccgg ggcagggggt cctcttgcgt caactggcac
     1081 tgtctgctcc cgcacatgcg attgcgttca gcgccgacgg cgcgatcctg gcggctggta
     1141 ccgtggaagg gggcgtccgt ctgtgggaaa cccagacctg gcgtgagttc tcaccctatt
     1201 caagcaggca aggtcggatc aacgcgttgc aattccttcc tggcaatcga tttctggccc
     1261 tcgctggagg acgatcgaaa gttcttgtcg tggatctggt cacgcgaacg ctcgtgaacg
     1321 aattggtgca taccagccct tctccggcct gcggcgaggg gagctgtaca aaaaggcgag
     1381 ctgtgcgcgg agaggtcata gatagtctca gcttcttgga cggaagctca tttcttttga
     1441 cgacctcgcc atccggtcct gtcgtctggg atatcacaac ttggcgcgaa gttgagaagc
     1501 cggccggcct tccggacgcc tggtccggtc ttggctggaa acattcattc gtctggacga
     1561 ccattcggtc cgctgttccg actgccttta ccctcgccgt atgggatacc aagaacaata
     1621 aagtgtgggc gagcctggat acgttcacca atcgggatac acaaatcgat catgggcccg
     1681 ccgtgtcgtt agggacatcg atggccgttg acccattgca tcgatgggct gccacccgag
     1741 tgggggaaca tatttccgtg tgggacctct cggcgcaagc caaacaaaaa acgttccatg
     1801 tcaaaactcc gtatcatctt cattggacga gcgacgggaa atatctgatt gtggccaccc
     1861 tcgaccgaaa aatcctcgtc tggtcagccg agaccatgga gccggtccac tatctgcgag
     1921 atccgtccgt tacccgctag ggatattcgt tcttcgcaag ggtctttttt catcttgtca
     1981 ttcaccatct ggtccattgt atccgcgata agttttgtgg taccaaacaa tgaacgtttg
     2041 tttcatgcga gggtgaatgg taaaaaagaa agaatccgca tcagatactg gatcgcaatc
     2101 ttccgctcct cgagcaaacc ctgaaccagc tgtaacgaag aaagtctccg ccaagccggc
     2161 ggaacgggtc actgcggtcg agatgggagc gcgtcagcgg gaaatctccg tctcggagtt
     2221 tttcacgaag aaccggcact tgcttgggtt cgacaacccg cgcaaggcgc tgttgacctg
     2281 cgtcaaggag gcggtggaca acgcgcttga tgcttgcgag gaggcgggga tccttcctga
     2341 tgtgacggtg aggcttgagg tagtgtccaa cggcgagccg gttgcgccca gccaagcgac
     2401 aaggtttcgt gtcacggtga ccgacaatgg tcccggcatc gttcgccagc agattcccag
     2461 gatctttgcg aagctgctct atgggtccaa gtttcatcga ttgcggatga gccgtgggca
     2521 gcagggcatc ggtatttccg ctgccgggat gtacggacaa cttaccaccg gcaagccggt
     2581 caagattatt tcccgtatcg gtcccaaagc cgccgcgcat ttcttcgaag ttcaaatcga
     2641 cacgaagaag aacgagccgc tggtccatga gaacaaacag atcgaatggg aacagccgcg
     2701 aggcacccaa gtcacgttgg aagtcgaagg caaatatcag aaaggccgag cctcggtcga
     2761 tgaatggctg gagcagacgt ccatcgccaa tccacacgtg aaactgatgt atcacactcc
     2821 ggaaggggag gtgaaagagt atccgcgtac ctatcacgag ctgccgccgc aaccgcgcga
     2881 gatcaagccc catccctacg gtattgaatt cggcatgctg ctcaagatgt tgcaggacac
     2941 gaagagccac acactctccg gctttctcgc cggcgatttt tgccgggtat ccccacagat
     3001 gggcgatgaa atctgcaaag cggccaaggt gtcaccgacg tcaaagccgc gcgatttgaa
     3061 ggggccggtg gcggagacac tctataagac gttgcaagaa acaaagatca tggcgccgcc
     3121 gaccaactgc atttcaccga tcggcgagaa ggcgatcctc tcgggactct acaaacagat
     3181 caagggagaa ttctatacgg cagtcagcag gccaccggcg gtctatcgtg gcaatccatt
     3241 catcatcgag gccgggcttg cctacggaaa tagacctcaa gatcaaaata agccgcagca
     3301 gccggccatg ccgaaagcgg aaggcgaaca cgaggaagac gactcggagc tcgctcgcgt
     3361 gatccgctat gcgaatcgag taccgctgct gtatcagcaa tcggcctgtt cgacgttcaa
     3421 ggccgttctg agcacaacct ggaaaaacta cggcttgacg cagtcgcgag gggctctgcc
     3481 cggtggaccg atggtgatct tcgtccatat ggcctcggtc tgggtgccgt ttacgagtga
     3541 atccaaagag gcaatcgccg actacgatga gattcaaaag gaaatcaccc tggcgcttcg
     3601 tgagtgcggc aggcggttgg gcctcttcgt tcgtcggcgc gaacgggcgg ccagcgaata
     3661 tcggcgtcgg aatatcttcg agctgtatat cgaagaagtc gtggacgcct gtaaccggct
     3721 caagggaggc aagctgccga aagaaaaact gaaagagcag cttca
//
LOCUS       DASTWE010001054         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_739123, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001054 DASTWE010000000
VERSION     DASTWE010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739123"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1294
                     /locus_tag="VFT30_10960"
     CDS             239..1294
                     /locus_tag="VFT30_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093199.1"
                     /translation="MAIGSIVASALRLSAGVLLCVVTVGCALTTGVDDRRLHMDDERR
                     CAPTDGDLALRRVSLTDIDTADDKVARMAEARAPNSFSWRVIQAAHAIEILPLLSRIE
                     ILEERQGTPQANPTQLNAIRNEVLGRIMLAMSEVSSTAAKTACEIERTYQAADRMRVG
                     EQARIKQQTLWAIIIGAAAAMVAGGATLSNAGSQAEGIASLIGGLVAGALGVTALYQE
                     GEEFFPHSNNILREVWVHSDQPRYIPPSIWLLLKQPVAEGSESKSYLEELIASWRQEG
                     RLGTPGSKTEQKRSELFFGPGGSYTTSDLVARAQMLDMLRATILVMNLHMERLIREVM
                     IQTAVRKQSLSPTRKNP"
     gene            1505..2026
                     /locus_tag="VFT30_10965"
     CDS             1505..2026
                     /locus_tag="VFT30_10965"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEU5093200.1"
                     /translation="MSKPKHIDADLLIRQYYEGEDTGPEGAFPEDTMESVSSVPPRSS
                     NTAMPGSTLSGGDVDAARSGTDAGEETVGGSTPTPDQDVVDELGKAVGITYNDAEPLQ
                     FGDKMADRDAQRWELNPASSEGYQERLQELRFRGTDKPKRPRNKQQKQTVRTEQEASS
                     RDKIARKTQKKRH"
     gene            complement(2126..2494)
                     /locus_tag="VFT30_10970"
     CDS             complement(2126..2494)
                     /locus_tag="VFT30_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093201.1"
                     /translation="MRRSPKKDLRFRLSYNSCSVSRSTDHLATEIRAPTALFRTWTAQ
                     GMLRRMTFTQRGTLIANLSAESAERVGPLRVAADPFGGEKANIRTIEAKPDATCHQII
                     MVVMFHADHVVRARLADLRA"
     gene            2493..2723
                     /locus_tag="VFT30_10975"
     CDS             2493..2723
                     /locus_tag="VFT30_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093202.1"
                     /translation="MRSFFVINLVFVVCLVTAPMQATTIVRLHEASPPTDPSPGPTDP
                     TPKPSGPIPPPQPPVPKPNPSHPSPSPVFGSA"
BASE COUNT          662 a          803 c          805 g          589 t
ORIGIN      
        1 catgagtgcc gcgacaatcc tcattgtcgt cttcgtgggc ggagccgtcg ggggactgtt
       61 cgggctgatc ttcgcgatac ccgtcgccgc ctgtctcaag atactcatcg aggaattcat
      121 cagaccgcgt tggctgcaat gggtgcatga ggcctagtcc aataccagaa agttctggtc
      181 agagcgtcaa gacgaatgag ccgaggataa cgaatgccta ggtggaggag aaggtgcgat
      241 ggcaataggc tcgatagtcg cgtcggcatt gcggttgagt gccggagtgc tgctctgcgt
      301 cgtgaccgtc ggctgcgcat tgacgaccgg cgtcgacgat cgccgtctac atatggacga
      361 cgaacgacgc tgcgcgccga ccgatggcga tctcgccttg cgcagagtat cgctcacgga
      421 catcgacaca gctgacgata aagtcgcccg aatggcggag gctcgcgccc cgaacagctt
      481 ctcctggcgc gtcatacaag cggcccacgc cattgaaatt ttgcctctcc tcagccggat
      541 cgagatcctg gaggagcgac aaggcacgcc gcaggccaac ccgactcagc tcaacgccat
      601 acggaacgaa gtattgggga gaatcatgct ggccatgagc gaggtgtcca gcaccgcagc
      661 aaaaacggcc tgcgagatcg aacgtaccta ccaggcggct gaccgcatgc gggtgggtga
      721 gcaggctcga atcaaacaac aaaccctatg ggctattatt atcggcgctg ccgccgccat
      781 ggtggccggc ggcgcaacct tgagcaacgc cgggtctcaa gctgagggga tcgcctcgct
      841 tatcggaggg ctcgtggccg gggcgctcgg agtgaccgct ttgtatcagg aaggcgagga
      901 gttttttcct cactccaaca acattttgcg cgaagtctgg gtccattccg atcagcctcg
      961 gtacattcca ccgtccatct ggttactgtt gaagcagccg gtcgcggaag gctcggaaag
     1021 caaatcgtat ctcgaagagc tgatcgcgag ttggcgtcaa gagggccgac tcggcacccc
     1081 cggcagcaag acagagcaga agcgcagtga gctgtttttc ggtcccggcg gctcatatac
     1141 cacctccgac ttggtggccc gcgcgcagat gctcgatatg cttcgcgcca cgattctcgt
     1201 gatgaatctc catatggaac ggcttatccg tgaagtcatg attcaaacgg cggttcgcaa
     1261 acaatctttg tcacccaccc gaaaaaaccc atgaccgact gcttgcctct caaacaccaa
     1321 tgtgtatgcc caggcttttc gtaataagtt gcggtggcga atctagggaa tgaccgagta
     1381 cagccttctt atgaacttgc atacattaaa agcgggtctt gtttatcgac actgagaatc
     1441 gaatctcaag ccggtacctt cacctatttt tcttatgagc tgaccacgca ttaaaggata
     1501 gaccatgtca aaaccgaaac acatagacgc cgatctcttg atccgccagt attacgaagg
     1561 agaggacacc gggccagagg gagcttttcc ggaagacact atggagtccg tttcgagcgt
     1621 ccctccgcgc tcctccaata cggccatgcc aggctcaaca ctgtccggcg gcgacgtcga
     1681 tgcggctcga agtgggactg atgcaggtga agagactgtg ggcggttcaa ctcctactcc
     1741 agatcaggat gtggtcgacg aattaggcaa ggctgtgggc attacataca atgacgccga
     1801 gcctttgcag ttcggcgata aaatggcgga tcgcgatgcg cagcgatggg aactgaaccc
     1861 cgcctcctcc gagggatatc aagaacgatt gcaggaactc agatttcggg ggactgacaa
     1921 gccgaaacgg ccgcggaaca aacaacaaaa acaaaccgtc agaaccgaac aagaagcatc
     1981 cagtcgcgac aagattgcca gaaagacaca aaagaaaaga cactgatatt ccgatatacg
     2041 gacaccatct ccacggaagg agccgacgat catgcaaaag aaaactttat cgccacagca
     2101 gcaaacctgt ctcgactcct gtttctcatg cgcgcagatc tgcgagacgt gcgcggacga
     2161 catgatcggc atggaacatc acgaccataa tgatttgatg acacgttgca tcaggctttg
     2221 cctcgattgt gcggatattt gccttctcac cgccaaatgg atcagccgca actcgcaacg
     2281 gtccgactct ctctgccgat tctgcgctga gatttgcgat caatgtgccg cgttgtgtga
     2341 acgtcatgcg ccgcaacatg ccttgtgcgg tccatgtgcg gaagagtgcc gtcggtgcgc
     2401 ggatctctgt cgcgagatgg tcggtactgc ggctcacgct gcatgagttg tatgagagcc
     2461 ggaagcggag atctttctta ggggaccggc gcatgcggag tttttttgtg attaatctgg
     2521 tgttcgttgt ctgtttagta accgcgccga tgcaggcgac aacgatcgtt cgactgcacg
     2581 aagccagtcc gccgaccgac cccagcccgg gaccgaccga cccgactccc aagccatccg
     2641 ggccgattcc gcctcctcag cctcctgttc ccaagcccaa tccatcgcac ccatcgcctt
     2701 caccggtgtt tggctcggca tgaggttgaa aggatttttc aatggtaccg acatactggg
     2761 aatcgctccg gattggatgg actatcctct ggcggggagc aggaagcgtg atgctgttgc
     2821 tgatgcttat caactggctg atcatcaccg tcatgcctg
//
LOCUS       DASTWE010001055         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_318826, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001055 DASTWE010000000
VERSION     DASTWE010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318826"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..>1003
                     /locus_tag="VFT30_10980"
     CDS             4..>1003
                     /locus_tag="VFT30_10980"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU5093203.1"
                     /translation="MTHFDHERIPERIVHARGSGAHGFFQVYEPMSRYTKAAFLNDPT
                     VQTPVFVRFSTVAGSRGSTDLARDVRGFAVKFYTQEGNFDLVGNNMPVFFIQDAIKFP
                     DLVHAVKPEPHQGMPQAASAHDTFWDFVSLMPESTHMLLWVMSDRAIPRSFRTMQGFG
                     VHTFRFVNDQGESTFVKFHWNPKQGTHSQVWDEAVKVSGADPDFHRRDLWEAIETGDF
                     PEWELGVQLFSEELADSFSFDVLDPTKIIPEELVPVTPIGRMVLDRNPDNFFAETEQV
                     AFCTAHVVPGIDFSNDPLLQGRLFSYTDTQLLRLGGPNFHELEINRPRCPMHNFQRD"
BASE COUNT          216 a          295 c          272 g          220 t
ORIGIN      
        1 aagatgacac attttgatca tgagcgaatt cccgaacgta ttgtacatgc acgaggctcg
       61 ggcgcccatg gatttttcca ggtatatgaa ccaatgtccc gttataccaa ggcagcattc
      121 ttaaatgatc cgacagtcca aacacccgtc tttgttcgat tttcaactgt agcgggctct
      181 agaggttcga cagatcttgc cagggatgtg cgaggctttg ctgtaaagtt ttatacacag
      241 gagggcaatt ttgacctggt gggaaataat atgcccgtat tttttataca ggatgctata
      301 aagtttcccg acctggtaca cgccgtgaaa cccgagcctc atcaaggcat gccccaagcc
      361 gcgtccgcac acgatacctt ctgggacttc gtttcgttga tgcccgaatc cacgcacatg
      421 ctgctgtggg tgatgtcgga ccgcgcgatt ccacgcagct tccgcacgat gcagggcttc
      481 ggcgtccaca ccttccggtt cgtcaacgac cagggcgaat cgaccttcgt caagttccat
      541 tggaacccga agcagggaac gcattcgcaa gtgtgggacg aagcggtcaa ggtttcgggc
      601 gccgatccgg acttccatcg acgcgacctt tgggaagcga tcgagacagg cgacttcccg
      661 gaatgggaac tcggcgtgca gctgttctcc gaggagctgg ccgacagttt ctccttcgat
      721 gtgctcgacc caaccaagat cattcccgaa gagctggtgc cggtgacgcc gatcggacgc
      781 atggtcctgg atcgcaaccc cgacaacttc ttcgccgaga ccgagcaggt ggccttctgc
      841 accgcgcacg tggtgccggg catcgacttc agcaatgatc cgctgctgca gggccgcctg
      901 ttttcctaca ccgacacgca gctcctgcgt ctcggcgggc cgaacttcca cgaattggag
      961 atcaacaggc cgcgctgccc gatgcacaac ttccagcgcg acg
//
LOCUS       DASTWE010001056         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_286880, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001056 DASTWE010000000
VERSION     DASTWE010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286880"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..>1075)
                     /locus_tag="VFT30_10985"
     CDS             complement(133..>1075)
                     /locus_tag="VFT30_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093204.1"
                     /translation="IVPAIAGKVSRLTLFMGKAQWILPRSDRHYGAAERFVRTLPGIR
                     HIIRLLVFVFYDIRFIAFRRYPGISGISQLMKDHYRRRLKKQLDKYIKDEKLRQHMMP
                     DYELGCRRVIPTNTYLPSLSMSNVDVDISGIECITPDGIRTKDGKDIGLDVIVYATGY
                     FAYSNMKKALTFQVHGLGGRNLNSEWEKEAVSYKGIIVSGYPNYFKVNGPNTGSGHSS
                     QISYMEAATNYIVQAICAVKRDQCIKAIDARRDLQDAYVADMRRKMQKTVWQNATCTA
                     FYRKNMTEEVTSLSPEPVTGFILSRRWFRLSDYRLLK"
BASE COUNT          232 a          298 c          295 g          250 t
ORIGIN      
        1 ccacacgtcg agacggctga agtcgactcg ctgctgctag tcactcaggg tgaggaccga
       61 aaagggtgga cacaggaagc caggttttcc gagtaactcc ctccgatcga ggagcgctcg
      121 cgcgatcgtc ctttacttga gcagacgata atcactcaag cggaaccacc tacgggaaag
      181 gatgaacccg gtcacgggtt ccggcgagag actggtgacc tcttcagtca tgttcttcct
      241 gtaaaaggcg gtgcaggtgg cgttctgcca gaccgtcttt tgcatctttc ttctcatgtc
      301 ggctacgtat gcatcctgaa ggtcgcgtct ggcatcgatg gccttgatgc actggtcccg
      361 tttcacggcg caaatggcct gaacgatgta attagttgcc gcctccatgt aggaaatctg
      421 cgagctgtgt ccggagccgg tgttcggacc attcaccttg aaatagttgg ggtaaccgga
      481 aacgataatg cccttgtaag agactgcctc tttttcccat tcactgttga ggttgcggcc
      541 acccagcccg tggacctgaa acgtcagcgc cttcttcatg ttcgaatacg cgaaatatcc
      601 cgtggcgtag acgatgacgt ccaggccgat atccttgccg tcctttgtcc tgatgccatc
      661 gggcgtgatg cattcaatcc cgctgatatc cacatcgaca ttgctcatgg acagcgatgg
      721 gagataggtg ttggtcggta tgacccgccg gcagcccaac tcgtaatcgg gcatcatgtg
      781 ctgccgcaac ttttcgtcct tgatgtactt gtccagctgc tttttcaacc ttctccggta
      841 atggtccttc atgagctgac tgattcccga aatccccggg taacggcgga aggcgataaa
      901 cctgatgtca tagaaaacaa agacgagcaa acggatgatg tgccgtatgc ccggcagcgt
      961 tcgaacgaaa cgctctgcgg caccgtaatg ccggtctgag cgcgggagaa tccactgcgc
     1021 cttgcccatg aacagcgtca agcgggatac cttgccggca atggccggaa cgatc
//
LOCUS       DASTWE010001057         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_60697, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001057 DASTWE010000000
VERSION     DASTWE010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60697"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFT30_10990"
     CDS             <1..501
                     /locus_tag="VFT30_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5093205.1"
                     /translation="YRAIHPIFGTMDDFDRLLESAHRLGIKVILDLVPNHTSSQHPWF
                     LESRASRNNPKRDWYIWRDPTLDGGPPNNWLSTFGGSAWEFDAGTSQYYYHAYLKQQP
                     DLNWRNPDVRHAMHEVMRFWLDRGVDGFRVDVIWHLIKDEQFRANPPNPHYRAGQWPY
                     RQLLAT"
     gene            471..1379
                     /locus_tag="VFT30_10995"
     CDS             471..1379
                     /locus_tag="VFT30_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5093206.1"
                     /translation="MALPTTACNITTDRPEVHHIIAGMRAVMDDYSDRLMIGEVYLPV
                     ERLVTYYGTGGDGCHLPFNFQLIEEPWHAATIASAVQTYEAALPAHGWPNWVLSNHDK
                     SRIGSRVGLPQARVAAMLLLTLRGTPTLYYGDEIGMTDVPIPPNLIQDPWEHNVPGIG
                     IGRDPVRTPMQWDASPHAGFTMGTPWLPVDQAASRVNIAKERNDPQSMLSLYRELIRL
                     RNDEDALILGSYRLLSVMDDVMVYLRELGTRRLLVGLNFSAQARVTSVQVRGKILLST
                     GLDRWNEQLDGELNLRGNEGVVADLE"
     gene            complement(1550..>1743)
                     /locus_tag="VFT30_11000"
     CDS             complement(1550..>1743)
                     /locus_tag="VFT30_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093207.1"
                     /translation="YIKLCYPETIVIGLSVNADKEKEEAMKRAGAVRLMTKEAAVEQL
                     YDAIQEAVRQREGRYANTL"
BASE COUNT          389 a          503 c          464 g          387 t
ORIGIN      
        1 tatcgagcca tccatccgat cttcggaacc atggacgatt ttgaccgact gctggaatcc
       61 gcccatcgtc tcggcatcaa agtgatcctg gacctcgtgc ccaatcatac gtcgtcacaa
      121 catccctggt ttcttgaatc gcgcgcttcc aggaacaatc ccaagcgcga ctggtacatc
      181 tggagagatc ccacactcga cggaggcccg cccaataatt ggctcagcac gttcggaggc
      241 agcgcctggg agttcgatgc aggcacgagt cagtactatt atcacgcgta cctcaagcag
      301 caaccggatc tgaattggcg caatccggac gtccggcacg ccatgcatga ggtgatgcgg
      361 ttttggttgg accggggtgt cgacgggttc cgcgtcgatg tgatctggca tctgatcaaa
      421 gatgagcagt tccgcgccaa cccgccgaat ccgcattatc gagcaggcca atggccctac
      481 cgacaactgc ttgcaacata acgactgatc gcccggaagt gcatcacatc atcgcgggga
      541 tgcgagccgt gatggatgac tattcggacc gactgatgat cggggaagtg taccttcccg
      601 tggagcgttt ggtgacctac tacggcaccg gcggtgacgg atgccatctg ccattcaact
      661 ttcaactcat tgaggagccg tggcatgccg ccaccatcgc atcagcggta cagacctacg
      721 aggctgcact accggctcac ggctggccta actgggtctt gagcaatcac gacaaatcgc
      781 gcatcggtag ccgcgtcgga cttcctcagg cacgtgtcgc agcgatgttg ttactgacct
      841 tgcgtggaac gccgacgctc tactatggcg atgaaatcgg catgacggac gtcccgatcc
      901 cgccgaatct cattcaagat ccctgggaac acaacgtgcc gggtatcggc atcggccgcg
      961 atccggttcg aacgccgatg caatgggatg cctcaccaca tgccggcttt acgatgggaa
     1021 caccgtggct acccgttgat caagcggcaa gtcgtgtgaa tatcgcgaag gagcgcaacg
     1081 acccgcaatc catgttaagc ctgtatcgag agttgattcg cctgcgcaac gacgaagacg
     1141 cattgatact cgggtcgtac cgcctcctgt cggtcatgga cgacgtgatg gtctacctgc
     1201 gggaactcgg cacacgtcgt ttgctggtcg gcttgaattt ttcagcacaa gcccgagtca
     1261 ccagcgttca ggttcgcggc aagatcctgc tttctaccgg tctggatcgt tggaatgagc
     1321 aactggacgg tgaattgaac ctacgcggca acgaaggcgt cgtagctgat ctcgaataag
     1381 agtgtccggt acagcgtgcg acttgcacta catccatagt gcctatccag ataatttttc
     1441 aaaactaggg gtcactccaa ttgggaaggt aacggtaaag aggataccgc gttttagttt
     1501 atggtgaaaa gttatagcag tggctcacca tcatgccatc atgagatatc tataaagtat
     1561 tagcgtatcg accttctctc tgtctcaccg cctcctgaat ggcatcatag agctgttcca
     1621 cagcggcttc cttcgtcatg agccggacgg ccccggctcg cttcatcgcc tcctccttct
     1681 ccttgtcagc attcaccgaa agtcctatca cgatcgtctc cggatagcac aacttgatgt
     1741 acc
//
LOCUS       DASTWE010001058         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_351644, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001058 DASTWE010000000
VERSION     DASTWE010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351644"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFT30_11005"
     CDS             complement(<1..814)
                     /locus_tag="VFT30_11005"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEU5093208.1"
                     /translation="MKIGKKDCQNLARALSLEWLETNGRGGFASGTVSGANTRRYHAL
                     LLTAQQPGTNRYVLVNHVEEWFYLDGEQVSLSTNLYPDTVHPQGYAHCTSFVSVPWSK
                     WIYTYKRQKIQREILCIRGRDLVFVRWKLMGSKGKDVALTVRPMLTSRDYHALHHENE
                     KLSMQATINPGWVTWQPYQGLPVVRAFHSGTYRHAPDWYRHVQFPLEQERGLDFGEDW
                     WSPGEFLFTLEPGKEQVLAFTSETVDRLDIADLAHKERVRRRRAQKSAPDVDR"
     gene            complement(1008..>1222)
                     /locus_tag="VFT30_11010"
     CDS             complement(1008..>1222)
                     /locus_tag="VFT30_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093209.1"
                     /translation="SRRLTHIFLRNKTGRRPVYGGANKFQEDPFWRDAILFYEYFHGD
                     NGAGIGASHQTGWTGLVAKLIQQSGE"
BASE COUNT          278 a          356 c          308 g          280 t
ORIGIN      
        1 atcggtcaac gtccggggca gacttttgcg cccgcctacg tcggacacgt tccttgtgag
       61 cgagatccgc gatatcaagc ctgtcaaccg tttcactggt aaatgccaac acttgctcct
      121 tgcctggctc caacgtgaat aaaaattctc ccggtgacca ccaatcttct ccgaagtcga
      181 gtcctcgctc ctgttctaga gggaattgca catgccgata ccaatcgggg gcatgacgat
      241 aggttccgga gtggaaggcg cgcacgaccg gcagaccttg atatggctgc caggtcaccc
      301 accctgggtt gatcgtggct tgcatggaaa gcttttcatt ctcgtggtgc aaggcgtggt
      361 aatcgcggct agtcagcatc ggccgcaccg taagagccac atccttaccc tttgaaccca
      421 ttaacttcca tcgaacaaaa acgaggtcac gcccccgaat acacaggatt tcacgctgaa
      481 tcttctggcg tttgtacgta tagatccatt ttgaccaagg gactgatacg aacgaggtgc
      541 aatgggcata tccctgagga tggactgtgt ccggatagag attagttgag agagaaactt
      601 gctcgccgtc tagatagaac cattcctcaa cgtgattgac cagcacgtac cgattggttc
      661 ccggctgttg cgctgttaac aacaatgcgt ggtaccggcg cgtattggca ccggaaacag
      721 ttcccgatgc aaatcccccg cgaccgtttg tctctagcca ttcgaggctc agtgcgcgag
      781 cgagattctg gcaatctttc tttccaattt tcatcttcgt ccacatctcg ggtggcacgg
      841 atggagcggt ttctcatcgc gcgcgatgtt ttcacccaca caaccccagc cgcgccgaga
      901 cgcacccctt cccacggaag cacctcccga tactgtgatt atgaacagat cgtgcgtgta
      961 cgttgggcga gcgaagaaac ggctcaggcc gtgtcaacct cacttcccta ctccccagac
     1021 tgctgaatca acttggcaac aagtccggtc catcctgtct gatgactggc cccaataccc
     1081 gcaccgttat ccccatgaaa gtattcgtag aaaagaatcg cgtctcgcca gaaaggatct
     1141 tcctggaatt tgttcgcgcc tccgtagaca ggacgccggc cggtcttatt gcggaggaaa
     1201 atatgagtca gccggcgcga ca
//
LOCUS       DASTWE010001059         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_836374, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001059 DASTWE010000000
VERSION     DASTWE010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836374"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFT30_11015"
     CDS             <1..419
                     /locus_tag="VFT30_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEU5093210.1"
                     /translation="ADLVIWKPEFFGVKPEMVLKSGFIAQAQMGDPNASIPTPEPTIS
                     RPMFGAFGRALSSTSFTFVSQAAVDHEIPKRLGLQRRIAAVKNCRSVKKRDLKLNDAL
                     PKVEVNPETYIVTADGVPLICEPAIVLPLAQRYFLF"
     gene            503..>1092
                     /locus_tag="VFT30_11020"
     CDS             503..>1092
                     /locus_tag="VFT30_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEU5093211.1"
                     /translation="MNTPALLEGLRFIDTFFPSGGYAFSSGLEAAIQGGAVKNSDQLA
                     RYVEDLLRGGMSRREAFAVKRANKAGSEGNISLAVDVDRELDSTKLSRESRMASRQMG
                     RQVIRVAADQLRAKTVLREYCDAMESEQAPGHLAVCFGLTLGVSGWEPEAAVAAFLYQ
                     TAVGFVSAAMRLSPIGQHEGQRILSEWLPLIDRISR"
BASE COUNT          237 a          266 c          339 g          250 t
ORIGIN      
        1 ttgccgacct tgtcatttgg aagccggaat tcttcggcgt gaaaccggag atggtgttga
       61 agagtgggtt catcgctcaa gctcaaatgg gcgatcccaa cgcgtcgatc ccgaccccgg
      121 agccgacgat cagtcggccg atgttcggtg cattcggtcg tgcactgagc agcaccagct
      181 ttacatttgt gtcgcaggcc gccgtagatc atgagattcc gaagcgcctt ggtttgcaga
      241 ggcgcattgc ggcagtgaag aactgccgta gcgtgaagaa gcgcgatctg aagctaaacg
      301 acgcattgcc gaaagtggag gtaaatcctg agacgtacat tgtgacggca gatggtgtac
      361 cgctcatctg tgaaccggcg atcgtgcttc cgctggcaca gcgatatttc ttgttttaat
      421 gaacctgagc agccggctcg gctacaagtc cggggatgat tgcgaaggta gctgaccggc
      481 tgttctgttt catcaatgat gtatgaatac tcccgcatta cttgaagggc ttcggttcat
      541 cgatacattt ttcccctccg gcggctacgc gttttcctca ggattagaag cggccatcca
      601 aggcggcgcc gtgaagaatt ctgatcaact ggcccgctat gtggaggatc tgttacgcgg
      661 cggcatgagc cgaagagagg ctttcgcggt aaaacgggcg aataaagccg ggtccgaagg
      721 aaatatctcc ttggctgtcg acgtcgatcg agaattggat tcgacaaagc tgagtcgtga
      781 gtcgaggatg gccagccgcc aaatgggtcg gcaagtcata cgcgtggcgg cggatcagct
      841 ccgggccaag acggttctcc gggaatactg tgatgcgatg gaatctgaac aggcgcccgg
      901 tcatctggcg gtctgctttg gattgacatt gggtgtcagt ggatgggaac ccgaagcggc
      961 cgtggcggct ttcttgtacc agacagcggt cgggtttgtg tctgctgcca tgagattgag
     1021 tccgatcggt caacatgaag gacagcgcat actcagtgaa tggcttccac tgatcgatcg
     1081 gatcagtcga ga
//
LOCUS       DASTWE010001060         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_642358, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001060 DASTWE010000000
VERSION     DASTWE010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642358"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFT30_11025"
     CDS             complement(<1..555)
                     /locus_tag="VFT30_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093212.1"
                     /translation="MTLVLLLGGCGGLQEVWEGPGARVFRPQTIAVLPPMASQYDSAR
                     EDIQDVLAGALNRHGNIERVVSPENVTDIFQASKEAFDSLVFYFSRLEMTGQSDKESA
                     IKLGKALNVDSFLVVRVNSWEYVRKEGDNVGRVGMSLRLIDATTGTTVWKARHERSSS
                     YMFIKPSLKDIAKELADEMIKYMPP"
     gene            complement(607..1749)
                     /gene="traF"
                     /locus_tag="VFT30_11030"
     CDS             complement(607..1749)
                     /gene="traF"
                     /locus_tag="VFT30_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HEU5093213.1"
                     /translation="MRCSPLLRIGVLLAASALPSHVSAAEFVIAGPRAMGMGGAGVAV
                     TTNALATYWNPAGLAMTQTVDIRIQGGGMAFDRLGLGDALHDLENFNTSDTTPDNLSR
                     AQSIADRINRSGATISVNGSAGLYLKGHLGEHAFGFNVSDVATGGGFVSTPVQASQGG
                     AGTPITVAGQMALRGLEARQLAFSYAYAFSDKTFSIGITAKVIQGAAYRGSTDLQGGS
                     GVSTTDHFGKPNISTTYGIDIGAIYRPSSWIRFGIVAKDINKPTFDAAEGGELKLEPQ
                     VRAGVAINPYSTLTLTADVDATSNKTFVPGVKSQLLSLGLEQTILSEFLSFRIGTFKN
                     MQDASTPFVPTAGLGIRWFNFRADAGGGYDFREKGALVSASISFTF"
     gene            complement(1908..2570)
                     /locus_tag="VFT30_11035"
     CDS             complement(1908..2570)
                     /locus_tag="VFT30_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5093214.1"
                     /translation="MIKVTNLHKSFSMGSYELPVLKGINLEIQRGELVAIVGASGAGK
                     STLLHIIGTLDKPTGGTVTFDGHDLFRMTDTQQAEFRNQRIGFVFQFHHLLAEFTALE
                     NTCMPSLVQRREPASVKADATSLLTEVGLGHRLHHKPGELSGGEQQRVAMARALMQNP
                     DLVLADEPTGNLDTHSGEGLFGLMRTLNKIRGTTFVIVTHNDKLSAQSDRIIHMQDGQ
                     IV"
     gene            complement(2609..>3728)
                     /locus_tag="VFT30_11040"
     CDS             complement(2609..>3728)
                     /locus_tag="VFT30_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5093215.1"
                     /translation="DIQSKILGTTAHIVVQERMKEHMTDYDRRTDKIQKVPDVVAATP
                     FVFRQVLLTTQSGVQGIILRGIDPKREANVTELAKNITAGQLLDLLTPVKVMQAPADD
                     PQGDLRPVEKPGIILGKELALRLGVFVGDTVNVVSPVGPISAMGMVPKIRTFAVVALF
                     HSGMFEYDSSFAYIELGEAQKFFNLGASVTGIEVKVTDVFRAGEIAHNIEQELGFSHG
                     ARDWMQMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQKEIAILKAM
                     GATKRSIRRIFMLNGLIIGFAGTGIGIPLGYAFLWLIQTFWTFDPSVYYISTIPVHVL
                     AEDVLLVAGSAILISFLATVYPANQAAKLEPVAALRYE"
BASE COUNT          837 a         1039 c         1020 g          832 t
ORIGIN      
        1 cggtggcatg tacttgatca tttcgtcagc gagctctttt gcgatgtcct tcaagctcgg
       61 tttgatgaac atatagctgc tggaacgttc atggcgtgct ttccagacgg tcgtgccggt
      121 cgtggcatcg atcaatcgta ggctcattcc gacccgcccc acgttgtctc cctccttccg
      181 cacgtattcc catgaattaa cccggaccac gagaaaggag tcgacgttga gcgctttccc
      241 gagcttaatc gctgactctt tatcggattg accggtcatc tccagacgag agaaataaaa
      301 cacgagcgaa tcaaacgcct cctttgaggc ctggaagata tccgtcacgt tctccggcga
      361 aacgacccgt tcgatattac catgacgatt gagtgctcca gccaagacat cctgaatatc
      421 ttcgcgtgcg ctgtcgtatt ggctcgccat cggcggcaac acggcgatgg tctgcggccg
      481 aaagaccctg gctccggggc cttcccatac ttcctgcaag ccgccgcaac ctccaagcag
      541 caaaacaaga gtcatcgcgg atattatgta cgcatatgat cgagacaaca tgggcttagt
      601 ataccattag aatgtgaatg aaatagaagc ggaaaccaag gcaccctttt cgcggaagtc
      661 atagccaccc ccggcatctg cacggaagtt aaaccaccgt atgcctaacc ctgccgtagg
      721 aacaaagggg gtggaggcgt cctgcatgtt cttgaacgtg ccgattctga atgacagaaa
      781 ctcagacaag atcgtttgtt cgagccccaa gctcaggagc tgacttttca ctcccgggac
      841 gaaggtcttg ttcgaagtcg catccacatc ggccgtcaat gtcagagtgg agtacggatt
      901 gatcgcgaca ccggcccgaa cctgtggttc caattttagt tccccaccct ctgcagcatc
      961 gaaggtaggt ttattgatgt ccttcgcgac gatcccgaac ctgatccatg acgatgggcg
     1021 atagatcgct cctatgtcaa ttccataggt cgttgagatg ttcggtttac cgaagtgatc
     1081 ggtcgtgctg acgccactcc ccccttggag gtcggttgaa ccacggtacg cggcgccttg
     1141 aatgactttc gcggtaattc cgattgaaaa ggtcttgtcg gaaaaggcat aggcatagga
     1201 aaaggccaac tgtctggctt cgagcccacg aagtgccatt tggcccgcca cggtgatggg
     1261 cgtgccagct ccgccttgag atgcctggac cggtgtcgag acgaatccgc caccggttgc
     1321 aacgtcggac acgttgaacc caaacgcatg ttccccaagg tgtcccttca gatatagtcc
     1381 ggcggaccca ttgaccgaga tcgtagcacc cgaccgattg atgcgatcgg cgatgctctg
     1441 agctctcgac aaattatccg gagtggtatc gctggtattg aagttctcca ggtcatgaag
     1501 cgcatcgccc aagccgagcc gatcgaatgc catcccgccc ccctggattc gaatatccac
     1561 ggtctgggtc atggccaagc cggcggggtt ccaataggtg gcgagcgcat tggttgtgac
     1621 ggcgacaccg gccccgccca tgcccatggc gcgaggacct gcgatcacaa attcggctgc
     1681 tgatacatga gacggcaacg ctgaggcggc cagcaacacg cctatgcgaa gcagtggtga
     1741 gcagcgcatc tcagatcctt gagcgaatgg acaccttggc gagtctagag aaggaacggt
     1801 agttcgtcaa ggaaacgccg ttggccaaca ccgcatgacg gcgcctgctc tagaaaaacc
     1861 ggagtcgaac tgaggcgaga ggacgctgcg acgggggtat ccgacaatca gacgatttgg
     1921 ccatcctgca tgtgaatgat gcggtcggac tgggcggaaa gcttgtcgtt atgcgtgacg
     1981 atcacgaacg tcgttccgcg tatcttgttc aaggtgcgca tcaatccaaa caggccttct
     2041 ccgctgtgtg tgtcgaggtt accggtcggt tcatcagcca agaccaagtc cggattctgc
     2101 atcaacgcac gtgccatggc gacccgctgt tgttcccctc ccgacagttc tccgggtttg
     2161 tgatgaagcc gatgtcccag tccgacttcc gtgagcaggc ttgtggcgtc ggccttgaca
     2221 gaagcaggtt cgcggcgttg cacgagcgat ggcatgcagg tattttccaa tgcggtgaac
     2281 tcggcgagca ggtgatggaa ctgaaacacg aaccctatcc gctgatttcg aaactccgcc
     2341 tgctgtgtat ccgtcatccg aaagaggtca tgcccatcga acgtgacggt gcccccggtc
     2401 ggtttatcca gagtcccgat aatatgaagt agggtgctct tgccggctcc cgaagctccc
     2461 acaatcgcca ccagctcgcc gcgctgaatc tcaagattga tccccttgag gacgggcagt
     2521 tcataggacc ccatcgagaa ggatttatgg aggttggtga ccttaatcat gatcaagtga
     2581 tgagttgtga ggactgagga ctgcgaatct attcataacg cagcgcagcg accggttcca
     2641 atttcgcggc ctgattggcc ggatagaccg tcgccaaaaa actgatcagg atggcggacc
     2701 ctgccacgag gagcacgtcc tcggccagca catgaaccgg aatagtggag atgtaataca
     2761 cgctcgggtc gaacgtccaa aacgtttgaa tcagccaaag aaacgcgtag cccagtggaa
     2821 tgccgatgcc tgtccccgca aatccgatga tcaatccgtt cagcatgaag atgcgtcgga
     2881 tactgcgctt cgttgcgccc atcgctttga gaatcgcgat ttctttctgc ttctctgtga
     2941 cgatcatcgt caaggtgctg acgatgttga atgaggccac gatcgtaatc aagacaagaa
     3001 ggagaaacat catcgtcttc tccagcttca gggcggagaa gagattgcga ttcatctgca
     3061 tccaatctct ggctccatga ctgaacccca actcttgttc gatattgtgg gcgatctcgc
     3121 cggcacggaa cacatccgtg accttgacct caattccggt tacactcgcg cctaggttga
     3181 aaaatttctg tgcctcgccg agttcgatgt aggcgaatga tgaatcgtat tcgaacattc
     3241 ccgaatggaa cagggcgacc acggcgaacg tccggatctt gggcaccatc cccatcgcgc
     3301 tgatcgggcc gacgggagag accacattga ctgtatcgcc gacaaacacc cccagcctga
     3361 gcgccaactc tttgccgaga atgatgcccg gtttttcaac cgggcggagg tcgccttgag
     3421 gatcatcggc cggagcctgc atcactttca caggagtgag caagtcgagc agctgtcccg
     3481 cagtaatgtt cttggcgagt tcggtgacat tggcttctcg tttcggatcg atcccacgca
     3541 agatgattcc ttgcaccccg ctctgcgtgg tgagtaatac ttggcggaac acgaacggcg
     3601 tggctgcgac gacgtcgggc actttctgga ttttgtcagt ccgacggtca tagtccgtca
     3661 tgtgttcttt cattcgctct tggacgacga tatgtgcggt cgtgccgaga atcttgctct
     3721 gaatgtct
//
LOCUS       DASTWE010001061         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1030480, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001061 DASTWE010000000
VERSION     DASTWE010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030480"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..435)
                     /locus_tag="VFT30_11045"
     CDS             complement(142..435)
                     /locus_tag="VFT30_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093216.1"
                     /translation="MTEESRVDYNLPMKALEALSQGDRSKAIEHIQMERNMSREDARE
                     LVAAFILSQPSLQAKMRMEENRAGTQWGLMRWLILFQAIAVAVGYFLFFRDQW"
     gene            complement(914..1081)
                     /locus_tag="VFT30_11050"
     CDS             complement(914..1081)
                     /locus_tag="VFT30_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093217.1"
                     /translation="MPTAEDSARRAALIPIGDHSDCPGEYSLTQHLVLAPDIRFQTIN
                     ATHFSGTQGLV"
     gene            1571..>2444
                     /locus_tag="VFT30_11055"
     CDS             1571..>2444
                     /locus_tag="VFT30_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEU5093218.1"
                     /translation="MNRLDPSPFILTINGGSSSIKFALFRDAESLVPVTRGMIERIGL
                     PNSEMLVTDAATQQSSRRSVHAPDHSACVGFITEWLEKRLDIGCLCAVGHRVVHGGKT
                     YSGPERVTPELLTELRRLCPYSPEHLPAEITLIESFRQYDTTLTQVACFDTAFHRDMP
                     RVARLLPIPRRYEKRGIQRYGFHGLSYAFLMRKLTRMGAPGEAEGRVILAHLGNGASM
                     AAVNHGQALDTTMGFTPASGLPMSRRSGDLDPGLVSYLARTEGMTVEQFHGMVNTESG
                     LLGMSELSSDMRDLL"
BASE COUNT          554 a          654 c          671 g          565 t
ORIGIN      
        1 aaatgcaaca caaagaaagg gggattaaaa gaagttgatg gcagtttgct tcttgttacc
       61 catttgaagg aggagtctgg aagagattga aggaggaggt tggaagagaa ggcttctcaa
      121 cgctgcttgt agcaggttgg ctcaccattg atctcggaaa aaaaggaagt acccgactgc
      181 gaccgcgatc gcttggaaca ggattagcca gcgcatgaga ccccactgtg tccccgctcg
      241 gttttcctcc atcctcattt tcgcttgtag agaaggttgc gagaggataa acgcggcaac
      301 cagttccctc gcatcctccc ggctcatgtt tctctccatc tgaatgtgtt cgatcgcttt
      361 gctgcggtcg ccttgcgaca gcgcctccag cgctttcatg ggaagattgt aatccactcg
      421 tgattcctcc gtcatggtga tcaatgaagt ctaccagaaa agatcgctgc ccgtcttctt
      481 gatttccaaa taacccgagt gcagtgcata gatgcagcac cctcgattat tccgttgtca
      541 gtttctatat gtcattctct atataatgtg tgcgccgtcc taagtccaca ctctcacact
      601 cgtatcgaag aggaggacca tcaggaatgt cgagaccgct ggagcagcac tggcggccca
      661 cctggatatc gacaaagtag gcttcaccgg ctccacggag ggggggaaag ctcatcctgg
      721 cgcggtgtcg ctggagcttg gagggaaatt gcccaacatc gtgtgcaaag acgccgatgt
      781 ggaagccagt atcccaggat ggcacgcgca atttttttaa ccacggccaa tgtgttgatt
      841 gaagttgtat tccacggccg gagcaatcgt gagcagattg ccatacccct tccgacgatc
      901 gccggttcgc cgttcagacc aacccctgtg ttccggaaaa atgcgtcgca ttgatcgtct
      961 gaaatcggat atcgggcgcg aggaccagat gttgcgtgag gctatattct cccggacaat
     1021 cagagtggtc accgatcggg atcaacgcgg ccctccgtgc cgaatcctcc gccgtaggca
     1081 ttgaatccct gcacccatga ccggtgagta gaaaccgtac gtcgtattca cgcggtactt
     1141 gaacacatga taccctcaca gccatatggc tttctgcact ccgatcccca gtgtcgtcgc
     1201 aaaagagccg cccaggtcca cccggaccct gcgaggcatt gaggcatatc tccccacggc
     1261 gagaagtctg tgtgccgcga atggcaacag tcgtccatta cattgaagaa tctgcaaaaa
     1321 gctttcccgc gtcgttctcg cagcctcaca ttccatatcg agcctgcccg atcgtgggtg
     1381 ttgaaatttg aacgagcgag ttcagcaggc gactaatgtc tcggcatttg tcttgctgga
     1441 atttgtttag ctgaagaaga gcatgatgcc gctgttgtag atgatcgttc ttcaacaacc
     1501 ggatgtcgac accgtatgca aacgaagcgc atgacgtgct ggacgcctgt tatggtaaag
     1561 cggtaacagc atgaatcggc tggatccgag cccgttcatt ctcacgatca atggcggatc
     1621 gtccagtatc aagtttgctc tatttcgaga tgcggaatcc ctcgttccgg tgacgcgcgg
     1681 catgatcgaa cggataggtc tgccgaacag cgagatgctt gtcaccgatg cggccacgca
     1741 acaatcgagc cgcagatccg tacacgcgcc tgaccatagt gcctgcgtcg ggtttataac
     1801 cgaatggttg gagaagcggc tcgatatcgg atgtctctgc gccgtcggac atcgtgtcgt
     1861 ccatggcggg aagacctata gcggacccga acgagtgacg cccgagttgt tgacggagct
     1921 tcgtcggcta tgcccttaca gccctgagca tctgcctgct gaaatcacct tgatcgaatc
     1981 gttccgtcag tatgatacga ctcttacaca agtcgcctgt ttcgatacgg catttcaccg
     2041 agacatgccg cgtgttgcgc ggctgctgcc gattccgaga cggtatgaaa agcggggtat
     2101 tcagcgctat ggatttcacg gattgtcgta cgcgtttctt atgagaaaat tgacgagaat
     2161 gggtgcgccg ggcgaggccg agggccgcgt tattctcgct cacttaggaa acggggccag
     2221 catggcggcg gtcaaccatg ggcaggcgct ggatacgaca atgggcttca cccccgcctc
     2281 cggtctgccg atgagtcgtc gatccggcga cctcgatcca gggcttgtct cttacttggc
     2341 ccgaacagaa ggcatgacag tggagcagtt ccacggaatg gtcaatacgg aatcaggttt
     2401 gctcggcatg tcagaattga gttcggacat gcgagactta ctca
//
LOCUS       DASTWE010001062         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_836387, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001062 DASTWE010000000
VERSION     DASTWE010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836387"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFT30_11060"
     CDS             <1..420
                     /locus_tag="VFT30_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093219.1"
                     /translation="TAAEPDFGQALRQLIASMSGAGQSTPELELTNPVLSFITPKLGE
                     QLLNIVREALSNSTRHAHASHRWVRLSLADNAIHLIIGDNGVGFSTKRKRRAGHGLSN
                     MAVRAKQISATFTLKSAPGKGTSVLVDIPLKKGNVYE"
     gene            413..1147
                     /locus_tag="VFT30_11065"
     CDS             413..1147
                     /locus_tag="VFT30_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5093220.1"
                     /translation="MNKSKPLRLVLVDDHEVVRIGLCAVLNLTPGMKVVGQGGRMEEA
                     KKLCYRLKPDLVLLDIRLPDGSGVEAARAILAKCPNTRVLFLTSFADDHTVLEAILSG
                     AQGYILKDIASNALIRAIRTVASGQALIDPRLTKHTLEWLSNVYSEPGQSKRPLLSPQ
                     EQRLLPLVANGLTNREIAHDLRLSEKTVKNYLANIYSKLNIGRRSQIAAFYAGSFKGS
                     GALYLSKLTESVKPDRAKTPPHLNSC"
     gene            1347..1916
                     /locus_tag="VFT30_11070"
     CDS             1347..1916
                     /locus_tag="VFT30_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEU5093221.1"
                     /translation="MSDSHPPESRDAHRSLKPSASNQVESFFESIVFTSRWIQAPLYA
                     GLIVAELLYAYKFLIELWEMVVHINKMEETVFMLGVLGLIDVTMVANLLTMVVIGGYA
                     TFVSKLDLEQHPDRPDWLTHVDPGTIKIKLAASLIGISSIHLLKGFVDVANENPEHLK
                     WKIFIHMTFLASAILLAWTDKIMQKDKKH"
     gene            complement(2020..>2796)
                     /locus_tag="VFT30_11075"
     CDS             complement(2020..>2796)
                     /locus_tag="VFT30_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093222.1"
                     /translation="DSARLGRLLSVLLSNAVKFTEHGEVCLFIEPAPRLIGANPASGS
                     SASRIRFSVMDTGIGIDTADVQRVFQPFVQADGSYRRKYGGVGLGLALAKQLVELMGG
                     TIDLESQPGKGSRFWFDLPLTRAGDRVPEPVEPGCALVYVKEIVSQAVIVKTLEKLGY
                     RVRTTTMAEELVNIGMELTPRLLVAEVDLLVSEADCIPVQMLKARFPELWILGLRHGA
                     MGGHPPLRLPFHMDGYLEKPLTVEAIKAAAAPKISKSPSH"
BASE COUNT          658 a          773 c          719 g          646 t
ORIGIN      
        1 accgcggcgg agccggactt tggacaggcc ctccgacagc tcatcgcatc gatgtccggt
       61 gccggccaat ccacacctga gctggagctc accaacccag tcctgtcctt catcaccccc
      121 aagctgggag aacaattact caatatcgta cgagaagccc tcagcaacag cactcgccat
      181 gcgcacgcgt cacatcgatg ggttcgactc agtctggcgg ataacgcgat ccacttgatc
      241 atcggcgaca atggagtcgg attttccacc aagcgcaaac gtcgcgctgg tcatggatta
      301 agtaacatgg ccgtccgagc caagcaaatc tccgccacgt ttacgctgaa gagcgcgccg
      361 gggaagggaa ctagtgtcct ggtcgatatt ccgctgaaga aggggaacgt gtatgaataa
      421 gtccaaaccg cttcggctgg tccttgtgga tgatcatgaa gtcgtgcgta tcgggctttg
      481 tgctgtcctc aatttgacgc ccggcatgaa ggttgtggga caaggtgggc gtatggaaga
      541 ggctaagaaa ctctgttatc gtcttaagcc tgacctcgta ttactcgaca tccgactgcc
      601 ggacggcagt ggggtggagg ccgcgcgcgc gattcttgcc aagtgcccca atactcgcgt
      661 gttgtttttg acgagtttcg ccgatgacca taccgtactt gaggctattc tttccggcgc
      721 tcagggctac attctgaagg atattgcgtc caatgcgctc atccgtgcca ttcggaccgt
      781 agcctctggt caggctctca tcgaccctcg cttgacgaaa catacactgg aatggctgag
      841 taatgtgtat agtgagccgg ggcaatcaaa gcgccctctc ctttctccgc aagagcaacg
      901 tcttctgccg ttggtagcca acggcttaac caatagagag atcgcccatg atctgcggtt
      961 gagtgaaaag accgtcaaaa actacctcgc gaatatctat tcaaaactga atattggacg
     1021 ccgatctcag attgccgctt tctatgccgg gagcttcaag ggctcagggg cactttactt
     1081 atcgaaactc actgaatcgg tcaaacctga ccgagctaag acacctcctc atctcaactc
     1141 ttgttaatac tcgtctgttt acggggaaag acgctgcccc attacagatc aaatcctcat
     1201 ccgcccaatc tctctctaca ccgttcgcgc ggcccacgaa caggacgaag cattgaattg
     1261 acagctttga attcactgac taggatacgc ctcgccgtcg agtcgtcact tgagggcacg
     1321 catcgttgga ctgctaggag gtcttgatgt ccgattctca tccgcccgag tcccgagatg
     1381 ctcatagatc acttaagccc tcggccagca atcaagtcga atcatttttc gaatccattg
     1441 tctttaccag tcgatggatt caagctccgc tctatgccgg actcatcgtg gctgaactgc
     1501 tctacgcata caaatttctc atcgagctct gggaaatggt ggtccatatt aacaagatgg
     1561 aagagacggt attcatgctg ggagtcctgg ggctgatcga tgtcacgatg gtcgctaatc
     1621 tgctgaccat ggttgtgata ggggggtacg caacgtttgt cagcaagctg gatttggagc
     1681 aacatccgga ccggccggac tggcttaccc acgtcgatcc cggcaccatc aagatcaagc
     1741 tggcggcatc cttgatcggc atctccagta ttcatttatt gaagggattt gtagacgtcg
     1801 ccaatgaaaa cccggaacat ctgaaatgga agatcttcat ccacatgacc ttcttggcct
     1861 ctgccatctt gttggcatgg accgataaga tcatgcagaa ggacaagaaa cattgagcaa
     1921 gggagggaac gaccttcttc atcgattgat cccacaattc gttctaggaa ccggtgccat
     1981 ttcccgtcat gatggaggga actggcagtg agaaactacc tagtgagacg gggatttgga
     2041 aatttttggt gccgcagcag ccttgatagc ttcgaccgtt agcggttttt ccagatatcc
     2101 atccatgtgg aacggcagac gcagtggtgg gtgcccaccc atcgcaccgt gtcgtagtcc
     2161 tagtatccac agttcgggga agcgtgcttt gagcatctgt acggggatac aatccgcttc
     2221 tgataccaga aggtcgactt ccgcaaccag cagtctcgga gtgagttcca tgccaatatt
     2281 taccaattcc tccgccatag tggtggtccg gactcgataa ccgagcttct caagcgtttt
     2341 cacaatgacg gcctgactga ctatttcctt cacatagacc agcgcgcagc caggttcaac
     2401 cggttccggc acacggtcac ccgcccgtgt cagtgggagg tcaaaccaga agcgactgcc
     2461 tttacctggt tggctttcga ggtcgatagt tccacccatg agttcgacca actgcttggc
     2521 aagcgccaaa ccgagaccaa cccctccata cttgcggcga tacgatccat ctgcttgaac
     2581 gaatggctgg aacactcgtt ggacatccgc cgtgtcgata cctattcccg tatccatcac
     2641 agaaaaccgg attcgactgg cgctggaccc agaagcgggg ttcgctccta tcagccgagg
     2701 ggcgggctca atgaagagac agacctcccc gtgttccgta aatttgacgg cgttgctgag
     2761 caagacggac agaagacgac caagacgagc cgaatc
//
LOCUS       DASTWE010001063         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1223849, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001063 DASTWE010000000
VERSION     DASTWE010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223849"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..>1014
                     /locus_tag="VFT30_11080"
     CDS             343..>1014
                     /locus_tag="VFT30_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU5093223.1"
                     /translation="MRYPFEERRHLGGLLVDRLLYWAVTKIRDRALKKGRIQHLFVPM
                     DDTLGMRVIATGEYESTHVAVMQALINSSHQLLGFKINTDAVCVDVGANIGLYSIFFS
                     GIFSHVLALEANPVTAKVLEANIAVAGRHNVSCICRGVSDSSGEVILHVPNDGNFGWS
                     TLERNQSVSNSNQLAIQTATLDDILASCEFAGEPVALIKIDVEGHELSVLRGSTRTLQ
                     RWNPIV"
BASE COUNT          210 a          259 c          298 g          247 t
ORIGIN      
        1 agggagtctt cgtggctgtc ttgatcgtgg agacgttatc tgctgtaggc ttcattgccg
       61 ccttgatcat tgaaaagtct ttcctgaggg ctatcgggac acaaccatga gaggcacgtt
      121 tgcatggccg cgaacggagc atcgggcgag agtgctgggg ttggcgaacc cgccgaacgg
      181 catacggtat tggtgcgctc gagcggcttg gcgccggact caatggtccg tagttcgcaa
      241 aaattgcttg ctgcggtttc ccggtgtatc aatttcgttg tgttcgggtt gctaggctgg
      301 ggcgcgaggg gccctgacta atcgcttcaa gcgaggaacg ccgtgcgata cccatttgaa
      361 gaacgccggc atttaggcgg actgctggtc gatagactgc tctactgggc agtgaccaaa
      421 atccgcgaca gggcgcttaa aaaggggcgt atccagcacc tatttgtccc gatggacgac
      481 acgttgggga tgagggtcat tgccacggga gaatacgagt cgacgcatgt cgccgttatg
      541 caggcattga ttaattcttc tcaccaactc ctgggcttca agattaatac cgacgcggtt
      601 tgcgtggacg ttggcgccaa tatcggcctg tactcgattt tcttcagcgg aatttttagt
      661 cacgttctcg cattggaagc caacccggtc accgcgaagg tcttggaggc gaatatagcc
      721 gttgccggac ggcataacgt atcgtgcatc tgccgaggcg tatctgattc gtcgggcgaa
      781 gttatcttgc acgtccccaa cgatgggaac tttggatggt cgacgctcga gcgaaaccag
      841 tccgtgtcca actccaatca actagccatc caaactgcta ccttggacga cattctcgct
      901 tcgtgtgaat tcgctgggga gccagtcgcc ttgattaaaa tcgacgtcga aggtcatgaa
      961 ttgtcagttc tccgcggctc gacgcgcact cttcagcgat ggaatccgat tgtc
//
LOCUS       DASTWE010001064         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_190025, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001064 DASTWE010000000
VERSION     DASTWE010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190025"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFT30_11085"
     CDS             complement(<1..314)
                     /locus_tag="VFT30_11085"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEU5093224.1"
                     /translation="MKIRSWSSASLALPMLFLLTGCPENGGGEGGFEASAPSNVVVSP
                     NGSFLYVSNRGLNSVSAYTIGGAGVITPITGSPFPAGTNPSAVTVSPNGSFLYVSNQG
                     SN"
     gene            468..1292
                     /locus_tag="VFT30_11090"
     CDS             468..1292
                     /locus_tag="VFT30_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093225.1"
                     /translation="MHHSLTILGSGYTAKFLLPLAQYSYSHVFATSRDPDRNLAHLSI
                     DQRIRFDLARPETWQAIPISTDIVWCFPAVPIELVQQFADAASLRTRRLVVLGSTSVY
                     DDCASVEYPPSWVDETTPIDLIKPRVQGEEFLRNSYGAVLLRVSGIYGPGRDPVEWIR
                     TGRVNRSRKYVNLIHVEDLASSCLAALLHADTGGIYNVSDGNPKTWMEICKMVEQRWN
                     IRSLDSQDADSPGKRVSNKRMCELLKLHGTGLRHGDLFEAIELIQRNSLNEATPSR"
     gene            complement(1267..>2017)
                     /locus_tag="VFT30_11095"
     CDS             complement(1267..>2017)
                     /locus_tag="VFT30_11095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5093226.1"
                     /translation="LVGIARLRRARMSILCLHLPGVPPPWPGGTAAAVILRGGALGVI
                     TTAVILSTSSFQHASLRALAIPLTYLPLLALAYVQLLRPAGLTFRNGFGLGIKRENLG
                     RLVCTIFAIVAAGLWGEWVMGRAVEFLNLNNHWTEWFDQDLVWGGPPVVAVSVLEYVI
                     FAPIFEELAFRGLLFAMLRRRFKFFPAALISTSLFALAHGYSLIGFLSVFWSGFLWAW
                     IYERTGSLIPGMIAHAANNLLVCLTVMALLR"
BASE COUNT          507 a          561 c          518 g          431 t
ORIGIN      
        1 ttgtttgaac cctggttgct cacataaagg aaacttccgt tcggtgagac tgtcacggcg
       61 ctgggattcg tccccgccgg aaatggtgat cctgtgattg gggttattac tccagcacct
      121 ccaatcgtat aggcggacac actgttcaat cctcgattgc tcacataaag gaaacttccg
      181 ttcggtgaga caactacatt gcttggtgct gacgcttcaa atcctccctc cccacctccg
      241 ttttccggac agccggtcaa aaggaatagc atcggcagag ccaacgatgc cgaggaccat
      301 gatcgtatct tcatatggtg cccttcttgt ttgcacacga agtgcgatag ttatttcccg
      361 tatagcacga ggaaatacgt acaccaacta aaatgacttt tttcgttgga ctctgatcgg
      421 ttctcgctgg cacactgttc acaggtcaac ttggaataca actgatcttg catcattcac
      481 tcaccattct cggatccggc tacactgcta agtttctcct gccgctcgcc caatatagct
      541 acagccatgt cttcgccacc agtcgagatc ctgatcggaa cctcgcgcat ctgagcattg
      601 atcaacggat acgattcgat ctggcacggc ccgaaacatg gcaggccatt cctatatcaa
      661 ccgatatcgt gtggtgcttt cccgcagtac cgatcgagtt ggttcagcag ttcgctgatg
      721 ccgcatctct acgaacgcgc cgcctggtgg tactcggcag tacctccgtc tacgacgact
      781 gcgcctcagt cgaataccct ccatcttggg ttgacgaaac aaccccaatt gacctgataa
      841 aacctcgcgt acaaggtgag gaatttttgc gaaattcata tggagccgtg ctgttgcgag
      901 tttcaggaat ctatggtcca ggccgcgatc ctgtcgagtg gatcagaaca gggcgagtga
      961 atcgctcacg taaatatgtc aacctgattc atgtcgagga cctggcctcg tcctgcctcg
     1021 cagccctcct gcacgctgac accggcggaa tctacaacgt cagcgatgga aatccgaaga
     1081 cctggatgga gatctgcaag atggtcgagc aacgatggaa tattcgatcc cttgactctc
     1141 aggatgccga ctcgccaggg aagcgggtgt caaacaaacg gatgtgcgag ttgctgaaat
     1201 tgcatgggac aggtctgcgc catggggatt tatttgaagc gattgagctg atacaacgaa
     1261 attcgctcaa cgaagcaacg ccatcacggt gagacacacg agcaagttgt tcgccgcgtg
     1321 ggcaatcatc ccgggaatca ggctccccgt ccgttcgtag atccaagccc agaggaatcc
     1381 gctccaaaag acgctgagga atccaatcag gctatagcca tgggccagag cgaaaagact
     1441 ggtactgatc agggcggcag gaaagaattt gaatcggcgg cgaagcatcg cgaacagcaa
     1501 tccacgaaac gcaagttcct cgaagatcgg tgcgaagatg acgtattcga gtacactgac
     1561 ggccacgaca ggcggacctc cccacacaag atcctgatca aaccattcgg tccagtggtt
     1621 attcaaattc aggaactcga ccgcccgtcc catcacccat tcaccccaga gtccagctgc
     1681 gacaatcgcg aagatggtgc ataccaggcg tccgagattc tctcgcttaa tcccgagacc
     1741 aaagccgttt cggaaagtca gaccggccgg cctgagcaat tggacatagg caagggcaag
     1801 cagtggtagg taggtcaaag gaatagccaa ggcgcgtaac gacgcatgtt gaaatgacga
     1861 agtcgacaaa atgactgcgg tcgtgatgac tcccagggca ccgccgcgga gaataaccgc
     1921 agcggcagtt cctccgggcc aagggggcgg aacgcctggc aaatgaagac acaggatgct
     1981 catccgtgct ctccgcagtc ttgctatgcc cacaagc
//
LOCUS       DASTWE010001065         1245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_545753, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001065 DASTWE010000000
VERSION     DASTWE010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545753"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..824
                     /gene="prcA"
                     /locus_tag="VFT30_11100"
     CDS             138..824
                     /gene="prcA"
                     /locus_tag="VFT30_11100"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEU5093227.1"
                     /translation="MPMPYYVSPEQMMQDKAEYARKGIAKGRSIIAMEYVDGILLTAD
                     NPSASLHKVSEIYDNIAFAGAGKYSEFENLRKAGIRHADLRGFMYSREDVTGRSLANG
                     YSQSLGTVFSQEMKPLEVEILVVQAGTNHHPNEIYRISFDGSIIDEKNFAVIGGRSEA
                     VQTVLKEKSPDQAPPLDTALKLCVTALEQTANQKLHPEGLEVAVLDRTRTGRKFRRLS
                     ISDVRHILSP"
     gene            897..>1245
                     /locus_tag="VFT30_11105"
     CDS             897..>1245
                     /locus_tag="VFT30_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEU5093228.1"
                     /translation="MMSAGPMKQRIFGLENEYGLIFSPNGRIYLPMEKVLGYIFEGLI
                     PNSWPSNAFLVNGARFYQDTGCHPEYSTPECDNILDLVIHDKAGERLLEACLPAAEER
                     LREEGLSGEIYIFK"
BASE COUNT          303 a          318 c          344 g          280 t
ORIGIN      
        1 ggaccggatc tcgtgcgggg catttacccc accgcaaaaa tcgtgaccgc ccaaggcatc
       61 accgacatat ccgacgacac gctacgtgcc gtgtgcaacg atgtgatggc ggcacgtcgt
      121 tcaagggaga cgtaatcatg ccgatgcctt attacgtgtc ccctgaacag atgatgcagg
      181 ataaggctga gtatgccagg aaggggattg ccaaaggccg ttcgattatc gcgatggagt
      241 atgtcgatgg tattctgttg acggcggata atcccagcgc ctccctgcac aaggtttctg
      301 aaatctatga caacatcgcc tttgcgggag ccggcaagta cagtgagttt gagaatcttc
      361 gaaaggccgg catccgccac gccgatctca ggggattcat gtatagccgg gaagatgtga
      421 ccggccgctc gctcgcgaac gggtattcgc aaagtcttgg aaccgtgttc agccaagaaa
      481 tgaagccgct tgaggttgag attctcgtgg tgcaagccgg caccaatcac cacccgaacg
      541 aaatctatcg tatttcgttc gatggcagca tcatcgacga gaagaacttt gcggtgatcg
      601 gcggacggtc tgaggcggtg caaaccgtgt tgaaagaaaa aagtcccgac caggctccac
      661 ccttggacac tgcgttgaaa ctctgtgtca cagcgctgga gcaaacggcc aatcaaaagc
      721 tgcatccgga agggttggaa gtagcggtgt tggatcgaac ccgtacgggc agaaagtttc
      781 gacggttatc gatcagcgac gttcgacata tcctctctcc ctgacctctg ccctccatag
      841 gggcttcctt tgttgaagat ccgaaaagcg gcgtataatc tgtcttaagg ccagtcatga
      901 tgtctgccgg tcccatgaag caacggatat tcggtctcga gaacgaatac ggcctcattt
      961 tttcccccaa cggtcgtatc tatctcccca tggaaaaagt tctgggatac atctttgaag
     1021 gattgattcc gaacagctgg ccgtcgaacg cattcttggt caacggagcg cggttctatc
     1081 aagacaccgg ttgtcatcct gaatactcga ctccggagtg cgataacatc cttgatctcg
     1141 tgatccacga taaagcgggc gagcgcttgt tggaagcctg tttgcctgca gcggaggaac
     1201 ggcttcgaga ggaaggattg tccggagaaa tctatatttt caaga
//
LOCUS       DASTWE010001066         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_609868, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001066 DASTWE010000000
VERSION     DASTWE010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609868"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1317)
                     /gene="gspF"
                     /locus_tag="VFT30_11110"
     CDS             complement(79..1317)
                     /gene="gspF"
                     /locus_tag="VFT30_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEU5093229.1"
                     /translation="MPVYQYQGYRNDGGAATGIIDAENVKVARLKLRKEGVYPTNVVE
                     QGQAPSRSKEKTSSHIERSIGRSTTLTSTDLALLTRQFATLLVAGLPLVEALGVLVDQ
                     AEKKPIKALLADIREQIRGGKALSVVLETYEKDFSPIYVHMVRAGETSGALDQILFRL
                     TEFLEKQLALRNKVTNAMLYPIIMLVIGSVILFFLITFVVPKITLVFAQQKQALPWPT
                     VALMSVSQFFADYWMVLVGLVLGGLYVTRRFVRTGAGRMMADRLILKSPLIGDVARMV
                     SISRLTSTLSTMLASGVQLLEAMDVSKRVMNNRVLEETVETARQNIREGETIADPLKR
                     SGEFPALVTHMIAVGEKSGEMEEMLRRVSQIYDGEVERVIARLTSLMEPIMILAMGAI
                     VLFIVVAILLPIFEMGQMIR"
     gene            complement(1413..>2058)
                     /locus_tag="VFT30_11115"
     CDS             complement(1413..>2058)
                     /locus_tag="VFT30_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5093230.1"
                     /translation="VEYQLLGIGQMQVNPKINLSFAAGLRSILRQDPDVIMIGEIRDR
                     ETAEIAIHASLTGHLVFSTLHTNDAASAATRLIDMGIEPFLVASSVVAVLAQRLLRRI
                     CPDCKRPYTPSEEELSRLDVAPGSDVTLYRGAGCAACSQTGYRGRTGIFELMVLDDEI
                     RRLIGSKADSSAIKQAAIAKGMVTLKQEGAERVIQGRTTLEEVMRITQQEIDVE"
BASE COUNT          425 a          591 c          576 g          466 t
ORIGIN      
        1 atcaactgag tttttccgca acctgtatct cattcaactt attcaattta tcggaccccg
       61 cattgagtat tgaccgccct atcgaatcat ctgacccatt tcaaagatgg gtaagagaat
      121 cgcgacgacg atgaagagga cgatggcgcc catggcaagg atcatgatcg gctccatgag
      181 cgaggtcaag cgggcgatga cccgttccac ttcaccgtcg tatatttgac tcacccggcg
      241 taacatctcc tccatttcac cgctcttttc accgacggcg atcatatggg tgaccagggc
      301 cggaaattcg ccgctccgct ttaaaggatc ggcgatagtt tccccttcgc ggatgttctg
      361 ccgtgcagtt tcgaccgttt cttcaaggac cctattgttc atgacgcgct tcgagacatc
      421 cattgcctcg agcaattgca ctccgctggc caacattgtt gacagggtgc tggtgagtct
      481 ggaaatcgac accatccgcg cgacgtctcc gatcaatgga gactttagta ttaaccggtc
      541 ggccatcatg cgaccggctc cggtccggac gaagcgccgc gtgacataga ggcctccgag
      601 cacgagcccc accaacacca tccagtagtc ggcaaagaac tgactgaccg acatcaaggc
      661 gaccgtcggc catggcagtg cttgtttctg ttgagcgaac acgagcgtaa tcttggggac
      721 gacaaacgta atgaggaaaa agaggatgac cgacccgatc acgagcatga tgatggggta
      781 gagcattgca ttggtgacct tgttcctcag ggcgagttgc ttctccaaga actctgtgag
      841 ccggaacaag atctgatcca gcgctccgct ggtctcgccg gctcgtacca tgtgaacata
      901 gatcggagaa aagtcctttt catacgtctc caggacgaca ctcaacgctt tccccccacg
      961 gatttgctcc cggatatcgg ccaggagggc cttgatgggt ttcttctcgg cttggtccac
     1021 caggacgccg agggcttcga ccaacggaag cccggctacg agtaaggtcg caaactgcct
     1081 ggtcaggagg gctagatcgg ttgaggtgag agtggtcgat cggccgatgg atcgctcgat
     1141 gtggctgctg gttttttctt tcgatcgact tggcgcttgg ccttgctcga cgacattggt
     1201 cggatagacg ccctctttcc tcagtttgag gcgcgcaact ttgacgttct ccgcgtcgat
     1261 gatcccggtc gctgctccac cgtcgttccg gtagccctgg tattgataga ctggcatggt
     1321 cggtcagcag gctccggacg catcgggttt ggccgatctg tgccggccag cccatgaacg
     1381 aaccgactgg aggaggcgat cagcgtgtcg cattattcga cgtcgatttc ctgttgagta
     1441 atccgcatga cttcttccag cgtggtgcgg ccttgaatga cccgctcagc cccttcctgc
     1501 ttcaacgtca ccatgccctt ggcgatcgcg gcctgtttga tggccgagga gtcggctttg
     1561 cttccgatga gccgccggat ctcgtcatcc aacaccatga gctcaaaaat accggtacgc
     1621 ccccgatacc cggtctggga gcaagccgcg cagccggctc ctcgatagag ggtcacgtcg
     1681 gaaccaggcg ccacgtccaa ccgactcagt tcttcttcgc tcggggtata ggggcgcttg
     1741 cagtccggac agatccttct cagcagtcgt tgggccagaa cggcgacgac tgaggaggcg
     1801 accagaaagg gctcgattcc catatcgatc aggcgcgtgg cggcactggc agcgtcgttg
     1861 gtatgcaagg ttgaaaacac cagatgtccg gtgagagagg catgaatggc gatctccgcc
     1921 gtttccctgt cgcgaatctc gccgatcatg atgacgtcgg ggtcttgccg cagaatcgaa
     1981 cgaagtccag cggcaaacga caaattgatt ttcgggttca cttgcatttg tccgatgccg
     2041 agcagctggt attccacc
//
LOCUS       DASTWE010001067         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_868834, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001067 DASTWE010000000
VERSION     DASTWE010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868834"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..495
                     /locus_tag="VFT30_11120"
     CDS             280..495
                     /locus_tag="VFT30_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093231.1"
                     /translation="MKSVDTRTDIQTDDPRNWMGGEATVGCQARRYIRRQAGHPTSLL
                     GVILSLTFLGGLALGSSFGLLCRSKGE"
     gene            719..>1171
                     /locus_tag="VFT30_11125"
     CDS             719..>1171
                     /locus_tag="VFT30_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HEU5093232.1"
                     /translation="MNRWTGKDGRLCQFSRPHKDVVADRKHPAQTGYVERLYEIPGLP
                     PENAQLIEDDFMQRLDSHAAAALTMLEDDDPRITREPQARSAWSRFIMSLLMRTPQDV
                     VAIKKGVAEEWARHIPGLEARYAAERMNDPITFDEYIARRGSDYINQRA"
BASE COUNT          294 a          303 c          318 g          256 t
ORIGIN      
        1 gttcaagtca ggagcttgct tcgggcaaat tctcatgaag tggaagggga ggccctcgat
       61 ctatcgctcg gctgcgccag gatcaaaagc tctctcgagg tccaaccggg aaggcagatc
      121 gccgtcaagc tgattgttcc aggagacgac actccgatct gattgaacaa gctcgggtac
      181 aatggaccgt cgatcgtttg tggacgtgca acagctagag atggatgaat tggagcagct
      241 gatcgatgaa tgcatcgcac ttgatgaagg gagagaggca tgaaatcagt cgatactcgg
      301 actgacatac agacagacga cccgcgcaat tggatgggag gcgaagcgac cgtcggttgc
      361 caagcacgtc gatatattcg ccgccaagcg gggcacccca cttcgctcct cggtgtaatc
      421 ctcagcctga ccttcctcgg cggcctcgcc ctaggcagct ccttcggcct actctgtcga
      481 agcaagggag agtagcttct tcctgccatc taattctgct cagcacttga ttaagtcacc
      541 agaggctggt tcgtatgcgt cggcctcaag gcctatctct gaagttttca aaaccaaact
      601 cctagacttc aattccgtca gctccaggtc tagcataagc tgcggataga agctgcagag
      661 ctaaaccgag tgtagccatg tcggtccctt tgaaacatca ccactgccgg tcttttacct
      721 gaaccgctgg acgggtaaag acggtaggct ttgtcagttc agtcgacccc ataaggatgt
      781 tgttgccgac cgaaagcacc ccgcgcaaac agggtacgtt gagcgtctct atgaaattcc
      841 gggtcttccg ccagaaaatg cacagctaat agaagacgac tttatgcaac gacttgactc
      901 gcatgctgct gcagcgctga cgatgttgga agatgacgat cctcggatca cacgggagcc
      961 acaggcaaga agtgcatggt cgcgttttat tatgtctctt ctgatgcgta ctccacagga
     1021 tgttgttgct atcaagaaag gagtagctga ggaatgggca cgccacatcc caggcttgga
     1081 agcgaggtac gcagccgaaa ggatgaacga tccaattaca ttcgacgaat atatcgcacg
     1141 gcgcggatca gattatataa atcagagggc g
//
LOCUS       DASTWE010001068         1506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1159891, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001068 DASTWE010000000
VERSION     DASTWE010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159891"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFT30_11130"
     CDS             <1..193
                     /locus_tag="VFT30_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093233.1"
                     /translation="SVSKEKPQSKASSDRNRAFFIFPPKNKLTLKGQSEGTVGKKLQK
                     NGYISETLFVLCVRDVAKI"
     gene            275..>1506
                     /locus_tag="VFT30_11135"
     CDS             275..>1506
                     /locus_tag="VFT30_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093234.1"
                     /translation="MNSWTKLLLLLPIISVTSNAQQIIRITEPTAISPAEVSIAINPK
                     NPDNMIAASFQTGRPPKPRAGSYHYVTFDGGKTWKTVPTPNPTNLVQGDDVLAFSNDG
                     VAYHVHLSFDGIRLARPVRAENGMIVNVSKDGGNTWTNGTAAINHVNTVIPFEDKPGL
                     VVDNAPGSRLKGNVYLAWTRFDVYGSADPEHRSQIYFTRSTDQGQTFSMPFRISDTGG
                     DCLDSDNTVEGAVPAVGPNGEVYVVWAGPLGLVFDKSTDGGLTFGKDKVIGDIPGGWD
                     FEIEGLSRANGMPVTGVDLSNGPNKGTLYVNWIDARNGDPDVFVMSSRDGGQTWTAPL
                     RVNDDALKNGKAQFFTWMAIDPADGSVNIVFYDRRDGKGTQTGLTLARSVDGGRTFVN
                     HKIDIPLFGVNERVFFGD"
BASE COUNT          366 a          385 c          415 g          340 t
ORIGIN      
        1 gagtgtgagc aaagagaagc cgcagagtaa agccagcagc gatagaaaca gagccttctt
       61 cattttccct cctaaaaaca aattgacact aaaggggcaa tcggagggaa ctgtcgggaa
      121 gaagctgcaa aagaatggtt atatttccga aactctgttc gttctttgtg tgagagatgt
      181 agcaaagatc taactattga caggatcata tgacatacgt gacagtctca tcttgttcat
      241 cctgcagatc ctgaaatcct gtaaacaact atctatgaac tcatggacca aactgcttct
      301 cctactcccc atcatctccg taacatcaaa cgcccaacag atcattcgca tcactgaacc
      361 aaccgcaatc agcccggctg aagtctcgat tgccatcaac ccgaagaatc ccgacaacat
      421 gatcgcggct tcgtttcaaa cgggccgtcc accgaagcca cgcgccggca gctatcacta
      481 cgtcacgttc gacggcggca agacatggaa aactgtgccc actccgaacc cgaccaatct
      541 cgtgcagggc gacgacgtgc tcgcgttcag taacgatggt gttgcgtatc acgttcacct
      601 gtcgttcgat ggaattcgtc tcgcgcggcc ggtgcgcgcc gagaatggca tgatcgttaa
      661 cgtctcgaaa gacggtggca acacatggac taacggcacc gccgcaatca atcacgtcaa
      721 caccgtcatt ccgttcgaag ataagcccgg tttggttgtc gacaatgcgc cgggctctcg
      781 cctgaagggc aacgtttatc tcgcctggac gaggttcgac gtttacggca gcgcggatcc
      841 cgaacatcgt tctcaaatct atttcacgcg ctcgactgat cagggacaga cgttctcgat
      901 gcccttccgc atctcggaca ctggcggcga ctgtctcgat agtgacaaca cggtcgaagg
      961 cgctgtgccc gcagttggtc caaacggtga ggtttatgtc gtgtgggctg gaccactcgg
     1021 tctcgtgttc gacaagtcga ctgatggtgg tctgacgttc ggcaaagaca aagtgattgg
     1081 agacattccg ggtggctggg atttcgaaat tgagggattg agcagggcca acggcatgcc
     1141 ggtgacgggt gtcgatctga gcaatggacc aaacaaggga acgctctacg tcaactggat
     1201 cgatgcgcgt aacggcgatc ccgatgtgtt tgtgatgtcg tcaagagatg gtggtcagac
     1261 gtggacggcg cctttgcgtg tgaatgatga cgcgctgaag aatggcaagg cgcagttttt
     1321 tacgtggatg gcgatcgatc ccgcggatgg atcggtcaat atcgtctttt atgatcgacg
     1381 tgatgggaaa ggcacgcaga ctggcctgac cctcgcgcgc agtgtcgacg gtggtcgaac
     1441 gttcgtgaat cacaagatcg acataccgtt gtttggggta aatgagcgtg tgttctttgg
     1501 tgatta
//
LOCUS       DASTWE010001069         1229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_448696, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001069 DASTWE010000000
VERSION     DASTWE010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448696"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFT30_11140"
     CDS             complement(<1..884)
                     /locus_tag="VFT30_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEU5093235.1"
                     /translation="MQLGRCITLVCFLIGLSGQHGDRAIAEESTVQSTSEWVTEIEKV
                     FIRSEDCKQCHERHYEEWKGAREQTPDLKTFGRVDAALLHGTSLESRVFRTVLGLWRQ
                     TNPTPDEQRRCLSCHVPSVNAFPQHAEKIATQVIAGTPKIEGIGCAACHLMNGMEKGL
                     NSPPTFKLQPGSTFYGPYSNPEENLVHPAALSDHFRAVSFCASCHFDKVKDAAQKDLP
                     GEILQGTICQDCHMEPSTGSSTSRRGAMTRAIGRHWFRGVVVSGTMLKNRNLQAEWMP
                     RIDVEVTKIDTAVEGTGIV"
     gene            complement(939..>1229)
                     /locus_tag="VFT30_11145"
     CDS             complement(939..>1229)
                     /locus_tag="VFT30_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5093236.1"
                     /translation="GAVDEEIQKELNLQKPQGVAVFEVIGNSRADYAGIKVRSVIKEI
                     DKQEIRNMADFGKAIKKAMKECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
BASE COUNT          272 a          374 c          285 g          298 t
ORIGIN      
        1 acaatgccgg ttccttccac cgccgtatcg atcttcgtca cctctacatc aatccgaggc
       61 atccactcag cttgtaggtt ccgattcttg agcatggtac cggaaacgac gacaccacga
      121 aaccaatggc gtccgatagc cctggtcatg gccccgcgtc gagacgtgga acttccggtc
      181 gatggctcca tgtggcaatc ctgacaaatc gtgccctgaa gaatctcacc cggtagatcc
      241 ttctgcgccg catctttcac tttatcgaaa tgacaggagg cacaaaaact gaccgctcgg
      301 aagtgatccg acaacgcagc cggatggacg aggttctctt cgggatttga ataagggcca
      361 tagaacgtgc tgccaggctg caatttgaat gtcggcggtg agttcagacc tttttccatt
      421 ccattcatca aatggcaggc tgcacagccg atcccttcaa ttttcggcgt gccggcaatc
      481 acttgcgttg caatcttctc cgcatgctgg ggaaatgcgt tgacggaggg cacatggcac
      541 gagagacagc ggcgctgttc atcaggcgtg ggattcgtct gcctccaaag acccagcacg
      601 gttcgaaaga cccgtgactc caacgacgta ccgtgcagga gggcggcatc cacacggcca
      661 aacgtcttca aatccggcgt ttgctctctg gcacccttcc actcttcgta atgacgctca
      721 tgacattgct tgcagtcttc agaacgaatg aagactttct cgatttccgt cacccactcc
      781 gacgtagact gcacagtcga ttcttcagca atcgcccggt cgccatgctg accgctcaga
      841 ccgatgagga agcagacgag tgttatgcag cgacctaact gcaccttacg gtcctgtcct
      901 gtccattcgc ctcacccctt aggtctcacc actcgccatt aatctcgttt acagccgaca
      961 aaatgaaaat tgacgcccca gccgaccgga tcgcctggat ctgccggttc ataggttccg
     1021 acggtaaagt tgcattcttt catcgctttc ttgatagctt tcccgaaatc cgccatgttt
     1081 cgaatctcct gtttatcgat ttccttaatg acggaccgaa ccttgattcc cgcataatcc
     1141 gcccgtgaat tcccgatgac ctcgaacaca gccactccct gcggtttctg caggttaagt
     1201 tctttttgaa tctcttcatc gacagcgcc
//
LOCUS       DASTWE010001070         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_836625, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001070 DASTWE010000000
VERSION     DASTWE010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836625"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="dnaX"
                     /locus_tag="VFT30_11150"
     CDS             complement(<1..483)
                     /gene="dnaX"
                     /locus_tag="VFT30_11150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU5093237.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQPHVVQTLMNAVSTKRIAHAYLFS
                     GTRGVGKTTVARILAKALNCERGPTSRPCDTCENCREIAQGNSVDVIEIDGASNTSVD
                     DVREIRENVKFTPFRGQFRVYIIDEVHMLSNSAFNALLKTLEEPPAHVVFIFATTEIH
                     K"
     gene            complement(588..687)
                     /gene="ffs"
                     /locus_tag="VFT30_11155"
     ncRNA           complement(588..687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFT30_11155"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(796..885)
                     /locus_tag="VFT30_11160"
     tRNA            complement(796..885)
                     /locus_tag="VFT30_11160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(849..851),aa:Ser,seq:gga)
     gene            1252..1334
                     /locus_tag="VFT30_11165"
     tRNA            1252..1334
                     /locus_tag="VFT30_11165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Leu,seq:tag)
     gene            1381..>2484
                     /gene="tig"
                     /locus_tag="VFT30_11170"
     CDS             1381..>2484
                     /gene="tig"
                     /locus_tag="VFT30_11170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU5093238.1"
                     /translation="MKMEVTELGPMKRALKIEVPADEVTQQFSRAYLELNRQVQIPGF
                     RPGKAPLAILEKRYAKTVEEDVIRKLVPDFYDRAIKQAGINPVVVEIPPLDRVKIKKD
                     SPFTFTATVEIKPTIELRDYKSPNPMSLQADKRTVAEEQVDRALDVFREQHARLDEAQ
                     TGTVLSEGDYAIVDLEGFLDGTPLDGTKKEGQLHKVGSKAALLGIEIDAHLIGRQAGD
                     IIEIPQTYPVSHPDQRVAGKAIKFRLVITGVKQKKLPILDDEFAKDCGPYATLHELRD
                     KLRDQMEKALKKDIEESYKDTILKRLIDTHHFDLPDTLVERELSTIVRQKLQARQRGK
                     VTDSIPASDAEELKRIREEHREEANRRVKAGLIL"
BASE COUNT          643 a          641 c          656 g          544 t
ORIGIN      
        1 tttgtgaatc tccgttgtcg caaagatgaa aaccacatgt gccggtggtt cttcgagggt
       61 cttcaacagc gcattaaacg ccgagtttga gagcatgtgg acttcatcga taatatagac
      121 tcgaaactga ccacgaaacg gcgtgaactt aacgttctcg cggatctctc gcacatcatc
      181 cacgctggtg ttggatgcgc catcaatttc gatgacatct accgagttcc cttgcgcaat
      241 ctcgcggcag ttctcgcagg tgtcgcaggg acgacttgtc ggtccccgtt cgcagttgag
      301 tgccttcgcg aggattcgcg ccacggttgt tttccccacg cctctcgtgc cggaaaataa
      361 atacgcatgt gcgatccgct tcgttgagac cgcgttcatt agcgtttgga cgacatgggg
      421 ctgaccgatt acatcgtcaa acgttccggg ccgatattta cgcgccgaga cttgatagtc
      481 cataaaagaa gccgtcaggg gtgagacgta aggggtgtaa agcaagacga catgaagccg
      541 aagatgttat tgccttacgt ctcacgtttc atacctcgtg tccagagtgg ggccaggtga
      601 tcctgcggca cacagaactg gctgcttacc gttgcttcct tccggacctg gcggggttca
      661 cagggttcta ttgcacaggg cccagcccct attttcgatc cagacctcca acacagtcca
      721 cacttgagat atctatccgc cccgcagaaa tacgatgctg cgataaagag cgggcggtac
      781 acacgaagtg cggtatggcg gagagggtgg gattcgaacc cacggtcccg ttgccgggac
      841 gcatgctttc caagcatgtc gattcgtcca ctctcgcacc tctccgcatc gagaagtgca
      901 aaggggtgac atcttagcat gcgtgttcct gaccggcaag gtgaccaacc ctcgccattg
      961 ccattaagtt tgagaggagg caattcctgt atcaagaaga gatcacctta agatgtacct
     1021 caagatgtac ctcgacgaac gtaatgtgtg agtttttcgg tgagcaacgg tgaccgatga
     1081 aagcagcaaa cagcctgact gggggtcatt catactagga atagggccct ctgtcgtatg
     1141 ggttgacatt agagaagccc aacggtacaa cgctatacga cacgcgtgtt ttgcggtctc
     1201 ggtcattcta ccgtttagct agagtgacag ctctgcggca tcctttacca tgcgagagtg
     1261 gcggaactgg cagacgcgcg agacttagga tctcgtgggc aaccgtgggg gttcaagtcc
     1321 cccctctcgc accacactac gattagtacg atccatttgg aagggagtcc gatcgcgacg
     1381 atgaaaatgg aagtgaccga gttaggaccg atgaaacgcg ccttgaaaat cgaggtgccg
     1441 gccgatgagg tgacacaaca attttcacga gcgtatttgg aactcaaccg tcaggttcag
     1501 attccaggat tccgaccagg aaaagcccct ttggctattt tagaaaagcg ttatgccaaa
     1561 accgtcgaag aagatgtcat tcgaaagctt gtgccggatt tctacgatcg ggccatcaaa
     1621 caggccggga tcaatccagt agtggtggag attccccctc tggatcgggt caaaatcaaa
     1681 aaagactccc cgtttacttt cacggcgaca gtcgaaatca aacccacgat cgaacttcgc
     1741 gactacaagt cccccaatcc catgtctctc caggccgaca agcgcacggt tgcggaagaa
     1801 caggtagacc gggcactgga tgtatttcgt gagcaacacg ctcgactgga tgaggctcaa
     1861 accggtacgg ttttatccga aggggattat gccatcgtcg atctcgaagg atttctggac
     1921 ggtactcctc tcgacggcac aaaaaaggaa ggtcagctcc ataaggtggg atcgaaagcc
     1981 gccctgctgg ggattgaaat cgacgcgcat ctgattggaa gacaggcagg ggatattata
     2041 gaaattcctc agacctatcc ggtaagtcat ccggaccaac gagtggcagg aaaagctatc
     2101 aaattccgtc tagtcataac gggagtcaag cagaagaagc ttcccatcct tgacgatgag
     2161 ttcgccaaag actgcggacc atacgcgacg ctccacgaat taagggacaa attgcgtgac
     2221 caaatggaaa aggccctcaa gaaggatatc gaggagtcct acaaggatac tattcttaaa
     2281 cgcctcatcg acacccatca tttcgatctt cctgacacac tcgtagaacg agagctcagc
     2341 acgattgtac ggcaaaaatt acaagcacga cagcgtggca aagtcaccga ttccattcct
     2401 gcatctgatg cggaagaact gaagagaatt cgcgaagagc accgcgagga agccaatcgt
     2461 cgcgtgaaag cgggattgat tctt
//
LOCUS       DASTWE010001071         1898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1418702, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001071 DASTWE010000000
VERSION     DASTWE010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418702"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /gene="fusA"
                     /locus_tag="VFT30_11175"
     CDS             complement(<1..1242)
                     /gene="fusA"
                     /locus_tag="VFT30_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5093239.1"
                     /translation="MARQTSLERTRNIGIMAHIDAGKTTTTERILYYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDSVQGVEPQSETVWRQADKYHVPRIAFMNKMDRIGADFYGSVQSIIDRLGAKPV
                     PIQIPIGREAEFRGSIDLVRMKGYFYDDETLGAKYKVDEIPGDLLEQAKEYREKMLDA
                     VAEFDDQVMEKYLNGHPLTEEEVMRAIRAATISMKVTPVLCGSAFKNKGVQQLLDGVV
                     DYLPSPLDIPPVLGVDPHSGKEVARKVDDGEPFAALAFKIMSDPFAGQLTFFRVYSGT
                     LKTGTPVLNVTKGTKDRIGRLLKMHANKREEIDEVHAGDIVAAVGLKGATTGDTLADE
                     KQPVLLEVMKFPEPVIAMAIEP"
     gene            complement(1357..1827)
                     /gene="rpsG"
                     /locus_tag="VFT30_11180"
     CDS             complement(1357..1827)
                     /gene="rpsG"
                     /locus_tag="VFT30_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5093240.1"
                     /translation="MPRSRFLGQREVLPDVRYRDKLVGKFINTLMSNGKKSTTERICY
                     GAFDVIQEKTGSDPLKVFKAAVDNVKPIVEVKSRRVGGASYQVPVEIRPARRVSLALR
                     WLSQFARTRGGKSMREKLAAELLDASNNTGAAVKKREDVHRMAEANKAFAHYRW"
BASE COUNT          435 a          578 c          417 g          468 t
ORIGIN      
        1 cggctcgatt gccatcgcaa tgaccggttc aggaaatttc ataacctcga gcaacaccgg
       61 ctgcttctca tctgccaagg tatctccggt ggtggccccc ttgagtccta ccgccgcgac
      121 gatgtctccg gcatgaacct catcgatttc ttctctctta ttggcatgca ttttcaagag
      181 acgtccgatc cgatccttcg tccccttcgt cacattcaag accggcgtgc cggtcttcag
      241 cgtcccagaa tagacacgaa aaaaggtcaa ttgaccggca aacgggtcag acatgatttt
      301 gaatgccaac gccgcaaatg gctcgccatc gtccaccttc ctcgccactt ccttgccgct
      361 gtgaggatct actcccagaa cgggaggaat atcgagcggt gacggcaaat agtcgacgac
      421 accatccaat agctgctgta cacctttatt cttgaaggct gagccgcaaa gcacgggagt
      481 aactttcatc gaaatggtcg cagctctgat cgcgcgcatc acttcctctt ccgtcaacgg
      541 atgaccgttc aaatacttct ccatcacctg gtcatcgaat tctgcgactg cgtcgagcat
      601 cttttcgcga tactcttttg cttgttcgag gagatcaccg ggaatttcat cgaccttgta
      661 cttcgcgccc agcgtctcat cgtcgtaaaa gtaccctttc attctgacca gatcgatgga
      721 tcctcgaaat tcagcttcac gtccgatggg gatctggatg ggaaccggct ttgccccgag
      781 tcgatcgata atggattgaa cgctgccata aaagtcagcc ccaatccggt ccatcttatt
      841 cataaaggca atacgaggga cgtgatattt atccgcctga cgccaaactg tctcagactg
      901 cggctcaact ccttgcaccg aatcgaatgc cgccaccgcc ccatcgagca cccgcagcga
      961 acgctctact tcgatcgtaa aatccacatg ccccggcgta tcaataatat tgatgcggtg
     1021 atcgcgccag aaacaggtgg tagcggcggc cgtaatggtg atgccccgct cacgctcctg
     1081 ctccatccaa tccatcgtgg ccgcaccctc atggacctcg cccatcttat gagtcatgcc
     1141 tgtataatag aggatccgct cggtggtcgt agtcttgccg gcatcaatgt gagccatgat
     1201 gccgatgttt cttgtgcgct ctaatgatgt ctgacgagcc acttcaatcc ctctaaaaac
     1261 aattgtgctg cagattaagc cgcatcacac tgctgcgatg tagaagcatc cggatctgcg
     1321 gatcgcagca cggctcaact gcagcacaga atgacgttac cagcgataat gagcgaacgc
     1381 tttgttggcc tccgccatcc gatgtacgtc ttcccgtttc ttgaccgcag cccccgtatt
     1441 attcgatgca tccaatagct cggctgcaag cttttcgcgc atactctttc caccgcgcgt
     1501 acgggcaaac tgcgataacc aacgaagcgc caacgacact cggcgtgccg gcctaatttc
     1561 gaccggaacc tgataggagg caccccccac ccgacgagac ttgacttcta cgatcggctt
     1621 cacattatcc acagctgcct taaacacttt cagcgggtcg ctaccggtct tttcctggat
     1681 aacatcaaat gcgccatagc atattcgttc tgtcgtactc tttttcccgt tactcatcag
     1741 cgtgttgata aacttcccga ccagtttatc tcgataccgc acatcaggaa gcacttcgcg
     1801 ttgacccaaa aacctactgc gtggcatatc gacctatcca atcaatgtaa tgattttcaa
     1861 aacccttgaa tctacttagg ccgctttgct ccatactt
//
LOCUS       DASTWE010001072         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_901642, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001072 DASTWE010000000
VERSION     DASTWE010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901642"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VFT30_11185"
     CDS             complement(<1..1431)
                     /locus_tag="VFT30_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093241.1"
                     /translation="MSIFRLLLWAVPWLGLLAIDFGTAAERPIPRVPLFDNLGTLHHP
                     ITTNSNEAQQYFDQGLRFVYAFNHEEAIMAFEEAARLDPSAAMAYWGVALALGPNINR
                     AMDKAAERRAWDAVQKARSHRAHASPAEQAYIDAIGKRYDLNTHARSALDKAYANAMR
                     LVWRQFPNDPDAGVLFAEALMDLSPWDLWTAAGRPKPGTEELLSTLESLLARFPDHPG
                     ACHYYIHSVEASPTPDRAVACAERLPGLMPGAGHLVHMPAHIYMRLGRYHEAAERNAH
                     AADVDRNYLSGRKLSGNYADGYYTHNLHFLWASLAMEGQRTEALKVARRLAGTITDDE
                     ARKDKWKELYLPTPLWSMIRFGQWDELLREPSPPKSLRLQQGMWRLGRGMALSASGRL
                     PGAEGEHVVLAGLAKRFGRDRTSEEKTERTLLKIAERLLAGELAMHRHKYDEGITSFT
                     DAVKLEEGLPYTEPPFWPIPTRHYLGA"
     gene            complement(1655..>2080)
                     /locus_tag="VFT30_11190"
     CDS             complement(1655..>2080)
                     /locus_tag="VFT30_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093242.1"
                     /translation="IQQDHLRPNVGGEPVQGAENPRASKVTILQKPVHAPTLVKSEEV
                     AELRAFLVGQQDEIARLTSHIQELTSLVSSQQQVLVHLGKELEAGAFSTTSTSTAAAS
                     VKRNRIGRKKSTENETPNSPQENEHQFSPQKSEGQSLNL"
BASE COUNT          436 a          586 c          589 g          469 t
ORIGIN      
        1 agcgccgaga tagtggcggg tcggaatcgg ccagaacgga ggttcggtgt atggtaaccc
       61 ctcttccagc ttgacggcat cagtgaacga tgtgatgccc tcgtcgtact tatggcggtg
      121 catcgcgagc tcgcccgcca acaatcgttc cgcgatcttg agcagggtcc gttcggtttt
      181 ctcctctgaa gtccgatcac ggccgaagcg cttagccaag ccggccagca ccacgtgttc
      241 accctctgct ccgggcaagc ggcctgacgc cgataatgcc attcctctgc ccagtcgcca
      301 cattccctgt tgcagacgca gcgacttggg aggagacggt tcgcgcagca gttcgtccca
      361 ctgtccgaac ctgatcatcg accacaaggg agtgggaagg tacaattcct tccatttatc
      421 tttccgcgct tcgtcgtcgg tgatcgttcc tgccagtcga cgtgccacct tcaaggcctc
      481 ggttctttgt ccctccatcg ctaaggaagc ccaaagaaaa tgcagattgt gggtgtagta
      541 gccgtcggca tagttgccgc ttaactttct tccgctcaga tagttgcgat ccacatccgc
      601 ggcatgtgca tttcgttcag ccgcctcatg gtatcgccca agtctcatgt agatgtgagc
      661 cggcatgtgg actaagtggc cggcacccgg cataagccca ggcaatcgtt ccgcacaggc
      721 cacggctcgg tcaggggtcg gagaagcttc cacgctatgg atatagtagt ggcaggctcc
      781 cggatgatcc gggaaacgcg ccagaagcga ctccaaggtg gacagaagct cctcggtgcc
      841 gggctttggc cgaccagccg ccgtccataa atcccacgga ctcagatcca tcaaggcttc
      901 agcgaacaat acgccggcat ccgggtcgtt cggaaactgt cgccagacta accgcatagc
      961 gttcgcatag gctttatcga gagccgatcg cgcatgcgta ttgagatcgt accgcttacc
     1021 gatcgcgtca atataagcct gctcggctgg gcttgcatgg gcacgatgtg aacgagcctt
     1081 ttgcacggca tcccacgccc tccgttctgc tgccttatcc atcgccctat tgatgttcgg
     1141 acccaacgcg agcgccacgc cccaataggc catcgccgca gagggatcaa gacgcgcggc
     1201 ttcttcgaag gccataatag cttcctcatg gttgaaggcg tacacgaatc gcaaaccttg
     1261 atcgaagtat tgttgagcct cattagagtt cgtcgtgatg ggatgatgaa gggtgccgag
     1321 attgtcaaag aggggcacac gcgggatagg gcgttcggcg gccgtgccaa aatcaatcgc
     1381 caacagacct agccacggca ccgcccacaa gaggaggcga aagatgctca ttgacggctt
     1441 tgtagcagat gtgaccgcac ctggctagca gatgttgatt tccacaggcg cgggctcgcg
     1501 aagtccgcga ggctcagatt tttaacggcc tgttaaaaga tggtgtacgt agtgagagga
     1561 tcaccggccc ggatcggtat gatttgctta ttttaccttc gaagtgcttc taaagaggcg
     1621 cgagcaacgg ccaacgctgg cggaccaatt cagcttacag attaagtgac tggccttcac
     1681 ttttttgagg ggaaaattga tgctcgtttt cctgaggaga attcggcgtc tcgttttcag
     1741 tcgatttctt ccgaccgata cggtttcgtt tgacggaagc ggccgccgtg ctggtcgagg
     1801 tcgtcgaaaa tgcaccggct tccagctctt taccgagatg aacgaggact tgttgctgag
     1861 agctgaccaa ggaggtgagc tcctggatgt gtgaagtaag ccgggcaatc tcatcctgtt
     1921 gcccgaccag aaaagctcgg agttcggcga cttcttccga cttaacaaga gtcggtgcgt
     1981 ggactggctt ttgtagaatc gtgacctttg aagcccgtgg attctctgct ccctggaccg
     2041 gctccccccc aacattcgga cggaggtgat cctgttgaat
//
LOCUS       DASTWE010001073         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1450942, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001073 DASTWE010000000
VERSION     DASTWE010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450942"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFT30_11195"
     CDS             <1..935
                     /locus_tag="VFT30_11195"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEU5093243.1"
                     /translation="DGQTVPGMLSKQHFTQPPPRYNEALLIKELEEKGIGRPSTYAAI
                     ISTIQDRKYVEKTEGRLVPTETGKTVNDFLLKGFPELINVDFTSQLEEQLDEVEEGNK
                     QWVAAVRDFYMPFTREMERAKTIPGPKDIVEPPTDIPCEKCGRMMEIKWGRNGKFLAC
                     PAYKDDPPCKNTQNFEKLPDGTIKIVPKQELTTDQVCEKCGSSMVVKTGRFGKFIACS
                     GYPQCKTTKPLALGVKCPQPDCGGDLVQKRTRKGRSFFACSNYPKCEFALWDRPVPKA
                     CPTCQAPFLIEKVSKQDGRSVQCRNQDCGYREAG"
BASE COUNT          262 a          277 c          290 g          206 t
ORIGIN      
        1 aggatgggca gacggtcccc ggcatgttgt caaagcaaca tttcacgcag ccgccgcccc
       61 ggtataatga agcgctcttg atcaaggagc tggaggaaaa aggcatcgga cgaccgtcga
      121 cctacgctgc cattatctcc acgattcagg atcgcaagta cgtggaaaag accgaaggac
      181 gcttggtccc cacggagacc ggcaaaacag ttaacgattt tctgctgaag ggttttcccg
      241 aactaatcaa cgtcgacttc acgtctcagc tggaagaaca gttggacgag gtggaagagg
      301 ggaataaaca gtgggttgca gcggtgcgcg atttctacat gccgtttacc agggaaatgg
      361 aaagggcgaa gaccattccc ggtcccaaag atattgtcga gccgccgacc gacattccgt
      421 gcgagaagtg cggacggatg atggagatca agtggggccg aaatggtaag tttctcgcct
      481 gcccggcata caaggacgat ccgccctgca agaacacgca aaactttgaa aagctgccgg
      541 acggcaccat caaaattgtg cccaagcagg aactgaccac cgatcaggtt tgcgagaagt
      601 gcggaagctc catggtggtg aagaccgggc ggttcggcaa gttcattgcc tgttcaggct
      661 atccgcagtg taagacgacc aagccgctgg cgctcggcgt caaatgtccg caacccgatt
      721 gcggcggcga cctcgtgcag aagcggacgc gaaaaggccg ctcatttttc gcgtgcagta
      781 attatcccaa atgtgaattc gccctctggg atcgcccggt gcccaaagct tgcccgacgt
      841 gccaggctcc attcttgatc gaaaaggtca gcaagcagga cggtcggagc gtccaatgcc
      901 gtaatcaaga ttgtggctac cgcgaagcag ggtgacgatt cgagattgtt tccttcactt
      961 tccttgcagt ccatcaatca ccacagtcct tacttcaccc gactaggatt cacctgacaa
     1021 gattgatgtc cattc
//
LOCUS       DASTWE010001074         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_255270, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001074 DASTWE010000000
VERSION     DASTWE010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255270"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /gene="nuoD"
                     /locus_tag="VFT30_11200"
     CDS             <1..1260
                     /gene="nuoD"
                     /locus_tag="VFT30_11200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU5093244.1"
                     /translation="FQLPPEKLAMAQEALQFKPEDWGFARTHHDPDFDYMFINLGPAH
                     TGTHGVLRLVTQLAGEEIIDIVPDIGFHHRAKEKIAERQTWHTFIPYTDRVDYLQGVL
                     NEMPYCLSVERLAGITVPERAQVIRVMMCEFYRLASHLVWYGTFAQDVGALSPVFYMF
                     NDRERIHAITEAICGGRMHPNWFRIGGVSHDLPVGWDKLVRQFVDYLPGRLDEYERLV
                     MDNPIFKQRTVGIGAFTLEDAIAWGATGPMLRACGLPWDLRKVRPYSGYDQFDFEVPV
                     GSHGDCYDRAVVHVLEMRQSLRIIRQCLEHMPTGPYKSLDPLATPPLKERTMQDIETL
                     IHHFLGVSWGPVLPPGEAEVPTESSKGQTSYYLTSDGAQVSYRTRIRTPSFAHIQMVP
                     FMARGELLPDLLAILGSVDFVLSDVDR"
BASE COUNT          280 a          401 c          370 g          295 t
ORIGIN      
        1 ttccaactgc cgcctgaaaa gcttgccatg gcgcaggagg ctttgcagtt caaacccgaa
       61 gactggggtt ttgcccgcac gcatcacgac ccggacttcg actatatgtt cattaatctc
      121 gggcctgccc atacaggcac acatggtgtc ttgcggctcg tcacacaact cgcgggcgaa
      181 gaaatcatcg acattgtgcc cgatatcggt tttcaccatc gagccaaaga aaagatcgcc
      241 gagaggcaga cctggcatac gtttattccc tataccgacc gtgtcgacta cctgcaaggt
      301 gtcctcaacg aaatgcccta ttgcctttcg gtcgaacgac tggccggcat tacggtccct
      361 gaacgggcgc aggtcatccg cgtcatgatg tgcgagtttt accgcctcgc cagccacctc
      421 gtgtggtacg gcaccttcgc gcaagacgtc ggcgctctct caccggtctt ttacatgttc
      481 aacgaccgcg agcggattca tgccatcacg gaagcgatct gcggcggtcg catgcacccg
      541 aactggttcc gtatcggtgg ggtttcccat gaccttcccg tcggatggga caaactggtc
      601 cggcagttcg tggactatct tcccggccgt ctcgacgaat acgaacgtct ggtgatggac
      661 aatccgattt tcaagcaacg gactgtcggc atcggcgcct tcacgctcga agacgccatc
      721 gcctggggcg ccaccggacc gatgttgcga gcctgtggac ttccatggga tctgcgcaaa
      781 gtccgccctt attccggcta cgaccagttc gactttgaag tccccgtcgg ttctcacgga
      841 gactgttacg accgggccgt ggtgcacgta ctggagatgc ggcaatcgtt gcgtatcatt
      901 cgccagtgcc tcgagcacat gccgaccggc ccctacaaat cactcgatcc tcttgccacg
      961 cctcccctga aggaacgcac gatgcaggat atcgagacac tcattcatca tttcctggga
     1021 gtgagttggg gaccggtgct ccccccgggc gaagcggaag ttccgacgga atcgtcgaag
     1081 ggccagacca gttattacct gaccagtgac ggggcacaag tttcgtaccg gactagaatc
     1141 cgtaccccat cgttcgcgca cattcagatg gttccgttca tggcgcgcgg cgaactcttg
     1201 ccggatctcc tcgccatatt gggaagcgtg gactttgtgt tgtcggacgt tgacaggtag
     1261 ggggaggcga gaaggcctta atcgtaaatc gtgaatcgca agggactccg gcgtttaacg
     1321 aacaacgttt aacgattaac gagcat
//
LOCUS       DASTWE010001075         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_125792, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001075 DASTWE010000000
VERSION     DASTWE010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125792"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          250 a          247 c          271 g          247 t
ORIGIN      
        1 ccagtccatc cagtccagct tatcaattgc gcgttttatt aataccgaaa tttgataagt
       61 caagaggagg caggacaatg acaactgatt ggtttttaaa gcaggattag ccgccagtct
      121 cctactagac tcaatcttcc tattgctatt ggcagataga ggatggagcc ctgccaagtc
      181 ttgtccctat cgctattccc agagggcatg tctggagtat tgacttttct tcagaaggaa
      241 ttattgccac aaccgttacg cggagtcgtc atgcttataa aggtgatgag gccgcatggc
      301 gatcttctac aatcagcagt cacgaaccgc catccatcat ttagaacagg accgtataag
      361 gtaacacccc tgcgaattgt cgctgctgaa cagcgggatg gccctgtgat tcaacaagtg
      421 caaaggtggg atgaatcgtg gaaccgtcgt ggagaacttc gaacacggtt ctgacaggga
      481 cggtcgatgt tggagccgtt cagcgcgcaa accaagatgc accgcggcag gctccaggct
      541 atggctttca gcatccagca gtattgctga atgtcccacc gccgaccata aaatttcgcc
      601 gctcactccg ctggtggtcg ctcgatcgga gaaggcggct ggcaacggtc attcgctttc
      661 gggatcaact ggttgatgac attatgcgtg cgggttgtag gaacgctggg tgaagaacag
      721 gagaccaatg atatgggaat cactgacgac gttaccgcgc accgaagttc tagggttggt
      781 gatattcgcc cttatcatct tggccttcat ggtgagtgcc tggctgtcat ggtggaagga
      841 caggagacgg acacagaagc gaacgttccg agtcaaatga ccgctcgatt tcgtcatgcg
      901 aaatcgctca ttcattccag cattccagtg gcaggtcatt gtggtcatgc atgatcccgc
      961 gctaatcacc gtacttgaca gagccgttca gaagcttatt gaaatcgtga gcttg
//
LOCUS       DASTWE010001076         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_158167, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001076 DASTWE010000000
VERSION     DASTWE010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158167"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="metG"
                     /locus_tag="VFT30_11205"
     CDS             complement(<1..1059)
                     /gene="metG"
                     /locus_tag="VFT30_11205"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5093245.1"
                     /translation="MANKNTFYITTPIYYVNDVPHIGHAYTTIAADVLARYWRLRGRE
                     VFFLTGLDEHGQKVQQAAAKAGIDPQVHCDKLAPKFETLWKKLNVSNNAFIRTTDSEH
                     TSIVQRYLQQLYDKKLIYKDSYTGWYCTFDERFWTEKDVVDRLCPDCKRPVEQLSEQN
                     YFFKMGQYQDRLVEHIKTHPNFIRPESRRNEVLGFLQNQKLGDLSISRPKSRLSWGIE
                     LPFDNNYVTYVWFDALVNYMSALEYLPREKPAGDRFWPANVHLVGKDILTTHAVYWST
                     MLMASNLPLPETIFAHGWWTVDGEKMSKSRGNVVDPNKMVEIYGVDAFRYFLLREVPF
                     GQDGDFSLTGMVARINSDL"
     gene            complement(1096..>1672)
                     /locus_tag="VFT30_11210"
     CDS             complement(1096..>1672)
                     /locus_tag="VFT30_11210"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HEU5093246.1"
                     /translation="TSRLHALPITIRSRCQALRFTTPARTQVEGALILKRELPPADAR
                     FLAVFTDGRIGEALTADVAEVRARQQECLTLVKREGLTSSATILSASESLAKADRGEE
                     TLAWLARWIRDLVIVMVDGDQDQILHLDQLADLRRYAQRADIDTLLTLLNDIERTEQQ
                     ATRHLNVQMALETTFLRLREALGLVPAGVSA"
BASE COUNT          358 a          443 c          486 g          385 t
ORIGIN      
        1 aagatcgcta ttgattcgtg cgaccatccc ggtaagcgaa aagtctccat cctgtccaaa
       61 cggcacctca cgcaggagga aatagcggaa cgcatcgacg ccgtagatct ccaccatctt
      121 gttaggatcg acgacgttgc cacggctttt ggacatcttc tcgccgtcca cggtccacca
      181 gccatgagcg aagatggttt caggtaatgg aagattcgat gccatgagca tcgtggacca
      241 atagacggca tgtgtcgtca agatatcctt cccgacaagg tggacattgg ccggccagaa
      301 tcggtcgccc gctggttttt cccgcgggag gtattccaag gcggacatgt aattgaccaa
      361 ggcatcgaac cagacatagg tcacatagtt gttatcgaat ggtagttcga tgccccagga
      421 gagcctggac ttcggacggg agatcgagag gtctccaagt ttctggtttt gaaggaaacc
      481 gaggacttcg ttgcgccgcg actctgggcg aataaaattt ggatgtgtct taatgtgctc
      541 gaccaagcga tcctgatact gccccatctt gaagaagtag ttctgctcgc tgagctgttc
      601 gacggggcgt ttacagtccg gacagagcct gtccaccacg tctttctccg tccagaaccg
      661 ctcgtcgaag gtgcagtacc atccggtgta tgaatccttg taaatcagct tcttgtcgta
      721 gagctgctgc aggtatcgtt gaacgatgga tgtgtgctcc gaatccgtcg tcctgatgaa
      781 ggcgttattc gaaacgttca gcttcttcca gagagtctca aatttcggtg cgagcttgtc
      841 gcagtggact tgtgggtcga tgccggcctt ggcggcagcc tgttgtactt tctgaccatg
      901 ttcatcaaga ccggtcagga agaacacctc gcgtccgcgc aggcgccaat agcgggccag
      961 cacgtcggcc gctatcgtgg tgtaggcatg gccgatatgc gggacatcgt tgacatagta
     1021 aatcggggtg gtgatgtaaa acgtattctt gttcgccata tcgagggaaa gataacaggt
     1081 tgagagcgcc tatttctagg cagagacccc ggcagggacc aacccgagtg cctcacgcaa
     1141 gcgaaggaac gtcgtttcca gcgccatctg cacgttcaag tgccgtgtgg cttgctgctc
     1201 ggttcgctcg atgtcattca agagggtgag aagagtgtcg atgtccgctc gctgggcgta
     1261 tcgccgcagg tcggcgagtt ggtcgaggtg aaggatctga tcctgatctc catccaccat
     1321 gacaatgaca agatcgcgga tccatcgcgc aagccatgcg agcgtttctt ctccgcgatc
     1381 cgccttggcc aagctttccg atgcagaaag gatggtcgcg ctcgacgtca atccttcccg
     1441 cttcaccaac gtcaggcatt cttgttgccg cgcgcgaact tctgcaacgt ccgccgtgag
     1501 cgcttccccg attcggccat cggtgaaaac cgccaggaaa cgggcatcgg caggaggaag
     1561 ctcccgtttg agtatcagcg ccccttcaac ttgcgtgcgt gcgggtgttg tgaaacgaag
     1621 cgcttggcag cgtgatcgaa tggtgatcgg aagtgcatgg agccggctgg tg
//
LOCUS       DASTWE010001077         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1515573, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001077 DASTWE010000000
VERSION     DASTWE010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515573"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFT30_11215"
     CDS             <1..440
                     /locus_tag="VFT30_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093247.1"
                     /translation="LADGLEYEYPDLSVRFHAQEGTAGLGGIVFGVRDTRNFYAAVVD
                     LIGQKVRLVHISDGRELPLAQASVNLKPVDWHSLRVQRNTIISKDFIEVVVDGVLVLS
                     VEDQALGLGQTGLVVLGSSTVLFDSFHAVPLFSHRPFSAPPAY"
     gene            703..1002
                     /locus_tag="VFT30_11220"
     CDS             703..1002
                     /locus_tag="VFT30_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU5093248.1"
                     /translation="MGAKIYVGGLPYSTTEQQLSDLFAAHGAVASARIITDKFTGQSR
                     GFGFVEMSSDAEAQAAITALNGTQLGGRTLTVNEARPQEPRSGGGGRGGFGGGRG"
     gene            1136..>1408
                     /locus_tag="VFT30_11225"
     CDS             1136..>1408
                     /locus_tag="VFT30_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5093249.1"
                     /translation="MPPFKLEAPFKACGDQPEAIARLTAGVQSGKKHQVLLGVTGSGK
                     TFTMANLIEQVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFVSY"
BASE COUNT          284 a          358 c          415 g          351 t
ORIGIN      
        1 tgctggccga tggattagag tatgagtatc ctgatctcag cgttcgcttt catgcgcagg
       61 aaggaacggc cgggctcgga ggcatagtat ttggtgtgcg cgatacgcgc aatttttacg
      121 cggcggtcgt cgatctgatc ggtcaaaagg tgcggctcgt tcacatctcg gatggacgcg
      181 aactacccct cgcgcaggcg tcggtcaatc tgaaaccggt tgactggcat tcgctcaggg
      241 tgcaacggaa caccattatc agcaaagatt ttattgaagt ggttgtagac ggagtgctcg
      301 tcctttccgt tgaagatcaa gccttgggat tgggacagac tgggttagtc gttcttgggt
      361 catcgaccgt gttgttcgac agtttccatg cagttcctct cttttcccac cggccgtttt
      421 ccgcgccgcc agcttactaa catgggctcg cctccgagcc tgtttggcct caaacttgcc
      481 ttttttcgtc ggtctggagt agcatggagc aaccggtttg cgaaaggcct ggcaagaccg
      541 gcgctgaaag tgacaaggcc tgaatggatg ttgtgttggg ccagtccggg aatctgatcg
      601 gaagccagag ctaagtcgcc gctgtgttga cgtcttcggc ccattccttc cccgttgttt
      661 ctcgagtcca agacaacatc attgtgtcaa aggaggagtc tgatgggtgc gaagatctat
      721 gtcggtggat tgccctattc aacgactgag caacaactaa gcgatttgtt tgcggcgcat
      781 ggggccgtgg cctcagcgcg gattattacg gacaagttca ctgggcagtc tcgcgggttt
      841 ggatttgtcg aaatgtcatc cgatgctgaa gcgcaagcgg cgattaccgc cttgaacggc
      901 acgcaactcg gtggtcgtac gttaacagtg aacgaagctc gtcctcagga gcctcgttcc
      961 ggtggaggag ggcgtggagg attcggaggc ggtcgcggtt agcctcaggt ctgttccgct
     1021 acgataaggg ctgccggagt ttccggcggc cctttttact ttttggatcg cgacgtcagg
     1081 aatcactgat gtccatttgg atttccgttc acattgtttc actttggaga ggaccgtgcc
     1141 accctttaag ttagaagctc cctttaaagc ctgcggggat cagccggaag ccatcgcacg
     1201 gttgaccgct ggggtgcagt ccgggaagaa acatcaagta ttgctcggag tgacgggttc
     1261 cggcaagacc ttcacgatgg cgaatctcat cgagcaagtc cagaagccga cgctcgtgct
     1321 ggtccacaac aagacgttgg ccggccaact ctatcaagag ttcaagcagt ttttccccca
     1381 caacgccgtc gagtacttcg tgagctat
//
LOCUS       DASTWE010001078         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_578648, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001078 DASTWE010000000
VERSION     DASTWE010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578648"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFT30_11230"
     CDS             complement(<1..524)
                     /locus_tag="VFT30_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU5093250.1"
                     /translation="MVSLVLFTPLLAALFVLLLRKEHEDGARWIAFSASLLTFFLSLG
                     LLWNFDSRADGFQLVERVPWIPDFGIEYHVGIDGISLFLVLLTTFLVPLVILSSWGDV
                     HKRVKEYQFLFLVLETGMIGAFVAIDLFLFYVFWEVMLIPMYFLVGVWGGARRIYAAI
                     KFLLYTMVGSLLMLV"
     gene            complement(518..>1630)
                     /locus_tag="VFT30_11235"
     CDS             complement(518..>1630)
                     /locus_tag="VFT30_11235"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5093251.1"
                     /translation="LIHAATMVTAGVYMIARLHFLYELSPTALACVSWVAAITAILAA
                     TIALVQTDIKKVLAYSTVSQLGYMFLGVGVGAYGAGIFHLMTHAFFKALLFLGAGSVI
                     HGMSEEQDIRKMGGLLGKMPITGWTFLIACIAIAGIPGFSGYYSKDLILEEAYAGPHA
                     QPLLYWVGTVGAGMTAFYMFRLFFVTFWGRDIRASHDVAHHVHESPWSMTIPLIILAV
                     LSVIGGYLPLLDWLSSLFHAGHVEVPDVIRWLPTIAGGVGIALAFFLYGTNTQQAVAK
                     MNEEPVGLRKALWNKWYVDEIYDAAIVKPTASLADVFWRGVDTRVIDGAVNGTARLME
                     LWASAIRVIQTGNVQNYAMSFLVGVTVILFLGVYLW"
BASE COUNT          445 a          468 c          430 g          287 t
ORIGIN      
        1 accagcatca acaggctgcc gaccatcgta tagagtaaaa acttgatggc cgcgtaaata
       61 cgccgcgctc ccccccatac gccgacgagg aagtacatgg gaatcagcat cacttcccag
      121 aacacgtaaa agaggaacag atcaatagcg acaaacgcgc cgatcatccc cgtctcaagc
      181 accagaaaga ggaactgata ctctttgacc cgcttatgga cgtcgcccca tgaggagaga
      241 atcaccaggg gcacaagaaa tgtcgtgagc agcactaaga agagactgat cccgtcaata
      301 ccgacgtggt actcgatgcc gaaatcagga atccacggca cccgctcgac cagctgaaat
      361 ccgtcagcac ggctatcgaa gttccacagc aggcctagtg acaagaaaaa cgtcaggagt
      421 gaggcgctaa acgcgatcca gcgcgccccg tcttcgtgct ccttgcgcag caatagcaca
      481 aacagtgcgg caaggagtgg ggtgaagaga acaagactta ccataagtac acccccaaga
      541 acaggatgac ggttacgccg accaggaacg acatcgcgta attctgcacg tttccggtct
      601 gtatgacacg aatggccgac gcccacagct ccatcaatcg tgcggtgcca tttaccgctc
      661 cgtcgatcac ccgggtatcg actcctctcc agaagacatc ggcaagggag gcggtcggct
      721 tcacgatggc tgcatcgtat atctcatcaa cgtaccattt gttccatagc gccttgcgta
      781 gcccgacagg ctcctcgttc atcttcgcca ctgcctgttg ggtattcgtc ccgtacagga
      841 aaaaggctag agcaatgccg actcctccgg cgatcgtggg cagccagcgg atcacatccg
      901 gaacttcgac atgtccggca tgaaagagcg aagaaagcca atcaagtagc gggagataac
      961 cgccaatcac agacaatacc gcgagaatta ttaaaggaat cgtcatcgac cagggggatt
     1021 catgcacgtg gtgagcgaca tcatggctcg cacgaatgtc tcgcccccag aatgtcacaa
     1081 aaaagaggcg aaacatgtag aaagcggtca taccggcacc gacggttccc acccaataca
     1141 gcaacggctg cgcgtgtggc ccggcgtacg cctcttcgag gatgagatct ttactgtagt
     1201 aaccagaaaa accagggata ccagcaatcg cgatgcaagc gatcaggaac gtccagcccg
     1261 tgatcggcat tttcccgagc aggccgccca ttttgcggat gtcctgttcc tcgctcatgc
     1321 cgtggatcac acttccggcg ccgaggaaaa gcagggcttt gaagaaggca tgggtcatca
     1381 aatggaaaat acctgccccg tacgcgccaa cccctacccc caagaacatg tacccgagtt
     1441 ggctgacagt tgaataggca aggactttct taatgtcggt ttggacgagg gcaatagtgg
     1501 cggcaagaat ggcggtgatc gcggcaaccc acgagacgca ggctaacgcg gtcggcgaaa
     1561 gctcgtacag aaaatgtaag cgagcgatca tatagacccc tgccgtgacc atcgtcgccg
     1621 cgtggatgag
//
LOCUS       DASTWE010001079         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_901768, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001079 DASTWE010000000
VERSION     DASTWE010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901768"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1307
                     /gene="prsR"
                     /locus_tag="VFT30_11240"
     CDS             <1..>1307
                     /gene="prsR"
                     /locus_tag="VFT30_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEU5093252.1"
                     /translation="LASDYDLLEASDRTSALSLIRQAKPPLVLLDLGLPPDVDGPSEG
                     LAILRETLRLNSEAKVIVVTGNSDRANAVAAIENGAYDFIEKPVQLDVLKVVLQRAMY
                     LFNLDRESRVIQGQVDENEFEGLVGNSKPMQDVFGMIRRVGPSDVQVLITGESGTGKE
                     LVARAIHRMSGRKEAPFVAINCGAIPEALLESELFGYEKGAFTGAVQQRKGRIESAQG
                     GTLFLDEIGDIPLSLQVKLLRFLQDHEVHRLGGKDTVSVDIRVLAATNVNLQEAIESG
                     RFREDLYYRLCVVVIPVPSLKARGSDITLLARTFLMKFAEAQNKMLKGFTPQAIEALL
                     AHNWPGNVRELENRVKRAVVMTEGKYVSPANLELRDLSSIERDPTTLRVARDLREKEL
                     VRFVLEKADGNVSKAAVELGISRPTLYQLLSRYGLRFPKFADR"
BASE COUNT          304 a          309 c          398 g          296 t
ORIGIN      
        1 cccttgcatc ggactacgac ttattggagg catcggatcg cacgagtgcc ctatcgctca
       61 ttcgccaggc caagccaccg ctggtgttgc ttgatttggg attgcccccc gatgttgacg
      121 gtccatccga agggctggcg attcttcggg agacccttcg actgaattcg gaggccaagg
      181 tgattgttgt cacagggaac agcgatcgag cgaatgccgt cgccgctatt gaaaacggtg
      241 catacgactt tattgaaaag ccggtgcagc tcgacgtgtt gaaggtggtg ctgcagcggg
      301 caatgtatct cttcaacttg gatcgggaga gccgtgtcat tcagggacag gtcgacgaga
      361 acgaatttga aggtctcgtg ggaaacagca agcctatgca agatgtgttt ggcatgatcc
      421 gtcgcgtcgg gccgtccgac gtccaggtcc tcattaccgg ggagagcggg acggggaaag
      481 aactcgtagc ccgtgcgatt caccggatga gcgggcgcaa agaagcacca tttgtcgcca
      541 tcaactgcgg cgcgattccg gaggcactgc tggagagcga gctgttcggc tatgagaagg
      601 gagcatttac cggggctgtc cagcaacgaa aaggccggat cgaatccgcg cagggcggca
      661 cgctcttcct ggatgaaatc ggagatattc cgcttagtct gcaagttaaa ttgctgcggt
      721 ttttgcagga tcatgaagtc catcggttgg ggggaaagga caccgtctcg gtcgatatca
      781 gggtccttgc tgcgacgaac gttaatctgc aagaggctat cgaaagtggt cgatttaggg
      841 aagatttgta ttacaggctc tgtgtagtgg tcatccctgt ccctagtctg aaggcgcggg
      901 gttcggatat cactctcctc gcacgaacat tcttaatgaa gtttgcagaa gcacaaaaca
      961 aaatgctcaa agggtttaca cctcaagcga tcgaggcgtt gttggcgcac aattggcctg
     1021 gaaatgtccg agaattagaa aatagggtca agcgggcggt tgtgatgacg gaagggaagt
     1081 acgtcagccc tgcgaatttg gaattgagag atctttcctc cattgagagg gatcccacga
     1141 cactccgagt cgcacgtgac cttcgcgaga aggagctggt gaggtttgta ctggaaaaag
     1201 ccgatggcaa tgtgtccaag gcagctgtcg aattggggat cagtcgtccc accctctatc
     1261 agctactctc tcggtatggg ttaaggttcc ccaaatttgc cgataga
//
LOCUS       DASTWE010001080         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1321385, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001080 DASTWE010000000
VERSION     DASTWE010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321385"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..>1056)
                     /locus_tag="VFT30_11245"
     CDS             complement(456..>1056)
                     /locus_tag="VFT30_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5093253.1"
                     /translation="AKKFHPDRIGEHSTEAFQAISEAYQALSDPEKRRLHNRLLDQEE
                     DRRREAGQRVKVRRGLEPEPLSWEKVSMMEDYQHAWPSFDGLYQQFLKDFTGMGVPNA
                     ARAEELNFEVILTADEARHGGVISVQVPTYYRCSVCGGIGRDWMFPCFDCQGSGVIET
                     ERPVNIRFPQGVRSGTVIEAPLSESGNANLHIRLYLVVD"
BASE COUNT          237 a          310 c          261 g          248 t
ORIGIN      
        1 ggtgaatgcg atagacttgt ctaatcgcgg aatgtgggat ctgggcaatc caaataaatg
       61 cccgccgctt gatcatgtgc ttccacccac tggggcttgc gtgtggtcgc ttgctcgaaa
      121 tctggggcgg cttgaggacc ctcttcataa tccggaattc tcgaccgcgg cgtaggaata
      181 aaaacgcttc gtctcggtgc agttcaagca cacgagtgta atcgataatg gcagcatcgc
      241 actgatctag cgcgtggtcg gattccgcgc ggctccgtca ttccagtcag cggtcctcga
      301 atgctcctcg aatgcagcaa gcctactcac ttcagccccg cgcaatcatc acgtctgtcg
      361 ccgacgtgga attcaccgca ttggataagt gctgctcaaa ccagctccat gaagccacat
      421 tgatcgaggt cagactcaag atctctggcc cttcgtcaat caactacgag gtaaagccga
      481 atgtgcaagt tagcattccc ggactcgctc agaggagctt cgataacggt tccgctccga
      541 acgccctgcg ggaagcggat gttaacaggc ctctcggtct cgatcacacc tgaaccctga
      601 caatcgaaac aaggaaacat ccagtctcgt cctattcctc cgcaaacaga gcagcgataa
      661 tacgtcggca cttgcacgga gatgactccg ccatgcctag cctcatcggc tgtaagaatc
      721 acctcgaagt ttagttcttc cgcccttgcg gcattaggca cccccatgcc ggtgaaatcc
      781 ttcagaaact gttgatacag tccatcaaag gaaggccaag catgctggta gtcctccatc
      841 attgagactt tttcccagct gagaggttct ggctccaggc ctcgtcgaac cttgaccctt
      901 tgaccagctt cacgccgcct atcttcctcc tggtcgagca aacggttgtg caatcgcctc
      961 ttctcggggt ccgacagagc ttggtaagct tctgatatcg cttggaaggc ctcggtactg
     1021 tgctcaccaa ttcgatccgg gtgaaacttt ttggca
//
LOCUS       DASTWE010001081         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1580192, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001081 DASTWE010000000
VERSION     DASTWE010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580192"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFT30_11250"
     CDS             complement(<1..384)
                     /locus_tag="VFT30_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093254.1"
                     /translation="MPKTHKEIFIARLMHERDKFELLLNRVGYTRRMTLKGVSGKWSI
                     KDMLAHILAYEQYIADRMHEILHGEEYVPCRTQNALDAFLDEYGYPDFGSPLLDDDGP
                     NEWVTEKYKNVALDDVVMQEIQAFSS"
     gene            complement(390..>1047)
                     /locus_tag="VFT30_11255"
     CDS             complement(390..>1047)
                     /locus_tag="VFT30_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093255.1"
                     /translation="DLCKRVLAEGRKVIGMKAKLNVGVSTFVPKSQTPFQWVSCDTPE
                     QIRAKQSLLRRELMRDRNIKLSLTKAEDSFLEAWLSRGDRRMAEVVYAAWKGGSKFDA
                     WDEGKKYEAWMQAFEEHGLDPLFYTHRQRRTDEVFPWEHITAAVRKNFLFQDFRQSLE
                     GQIRVDCRLNCFACGILPTFANMRRENPGDVWKCPDVKSPVSTNRAAMSSLKLPVVGD
                     "
BASE COUNT          216 a          287 c          286 g          258 t
ORIGIN      
        1 cgatgaaaat gcctggatct cctgcataac cacatcgtcc agagccacgt ttttatattt
       61 ctcggtgacc cattcgtttg gtccatcatc atcgagcagc ggcgagccga aatcgggata
      121 tccatattcg tcgagaaagg catccagtgc gttctgagtc cggcagggca cgtattcttc
      181 gccatgcagg atctcatgca tccggtccgc tatatattgt tcgtaggcaa ggatatgagc
      241 aagcatgtcc ttgatggacc acttcccgga aacgcccttc aacgtcatgc ggcgtgtgta
      301 tccgaccctg ttcaataata gctcaaattt gtcgcgctcg tgcatgagac gcgcgatgaa
      361 aatctcttta tgtgtcttgg gcataacctt taatctccca cgaccggcag tttcaggctg
      421 gacatggcgg cccggttcgt ggaaaccggg gatttgacgt cagggcactt ccatacatcg
      481 ccgggattct cgcggcgcat gttggcgaag gttggaagga tgccgcaggc gaaacaattc
      541 aagcggcaat ccacgcggat ttgtccttcg agggattgcc tgaaatcctg gaacaggaag
      601 ttcttgcgaa cggcagcggt gatgtgctcc caggggaaga cttcgtcggt gcggcgctgg
      661 cggtgagtgt aaaagagcgg gtcgagccca tgctcttcaa aggcctgcat ccacgcttca
      721 tactttttgc cttcatccca tgcgtcaaac ttggagccac ctttccacgc ggcataaacg
      781 acttctgcca tgcgccggtc gccgcgtgag agccaggctt ccaggaatga atcctccgcc
      841 tttgtcaggc tgagcttgat attgcggtcc cgcatcagtt cccggcgcag caaggactgt
      901 ttggctcgga tttgttcggg tgtatcgcag gagacccatt ggaagggggt ctgcgatttc
      961 ggcacgaacg tactgacccc tacattcagt ttcgctttca tgccgatgac cttgcgtcct
     1021 tccgccagta cacgtttgca caggtct
//
LOCUS       DASTWE010001082         1004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_93453, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001082 DASTWE010000000
VERSION     DASTWE010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93453"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..957
                     /locus_tag="VFT30_11260"
     CDS             376..957
                     /locus_tag="VFT30_11260"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093256.1"
                     /translation="MDISRRTFIKTTLIGGAGVSVFGFNLAPVYAQTQGLKIERTTET
                     RSTCPYCSVSCGVIIHTLGDKAKNAVPQVVHVEGDPDHPINRGTLCPKGASLEQEIVS
                     DRRLVKPQVRRPGSDHWEDISWDQAIDDIARWTKKTRDATFIEKDKDGYTVNRLETIG
                     WIGGCTDTNELNFLAVKTMRSLGLCYFETQARV"
BASE COUNT          244 a          265 c          265 g          230 t
ORIGIN      
        1 ttgaggcgtg cgatacgtgc aaacactata tcaagggaat cgatctcact cggttcgggt
       61 tcgctgcgcc cctcgttgat gaactctacg ccgcgccgct ctatctctgg gcacgggaac
      121 acgggtatac gaagatcgaa ctcaatttgg tgggcttatg aaacgagttt atttagacag
      181 gatgacatga tttacaggat gacaagataa atcctgtgaa tcctgtaaat cttgtaatcc
      241 tgtctaaatt acgtggccta ctttaaacgt tgactttgct ggtgaatgct cctagtctta
      301 tccccgtaac cccctcaatt tggagcccct aacgatcatt agtcaccggt aattccgttg
      361 acggaggtat ggtctatgga tatctccaga agaaccttca tcaagacgac cttgatcgga
      421 ggcgcaggtg ttagtgtatt cggcttcaat ctcgctccgg tgtacgcgca gacacagggt
      481 ctgaaaatcg agcgtaccac cgaaacgcgc agcacgtgtc cttactgttc cgtaagctgc
      541 ggagtgatca tccacaccct cggcgataag gccaagaacg cggtaccaca agtggtccac
      601 gtcgaaggcg atccggatca cccaatcaat cgcggcacgt tgtgtccaaa aggcgcttcg
      661 cttgagcagg aaatcgtcag cgatcgtcgc ttggtgaaac cacaggtgcg acgtcccggc
      721 tctgatcatt gggaagacat ctcatgggac caggcgattg acgacattgc tcgttggacc
      781 aagaagacac gcgacgccac gttcatcgag aaagataagg acggctatac ggtcaaccgg
      841 ttggaaacga tcggttggat cggcggctgc actgacacaa atgaactgaa ttttctcgcc
      901 gtaaaaacca tgcgcagcct cgggctctgc tatttcgaaa cgcaggcccg ggtttgacac
      961 ggtccgacgg tctccagttt ggggcccacc ttcggacgcg gcgc
//
LOCUS       DASTWE010001083         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_707719, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001083 DASTWE010000000
VERSION     DASTWE010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707719"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFT30_11265"
     CDS             complement(<1..277)
                     /locus_tag="VFT30_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5093257.1"
                     /translation="MPGQAPDDAAPHDAFKTDQVADLKSPELAPYWLAALIESAEDAI
                     ISKTLDGIITSWNHGAQRIFGYTADEAIGKPVTMLIPPDHIDEEPAIL"
     gene            complement(456..>1054)
                     /locus_tag="VFT30_11270"
     CDS             complement(456..>1054)
                     /locus_tag="VFT30_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093258.1"
                     /translation="IDPDSIKTALTYEKIFVFGRTRGLILRSDAFGFEFDKRNKNRNI
                     MTDLHGILVLPPHRFNENNIAAVDFTAGFEAGHNFKHALNPNDGLGNLWRWKFGANAY
                     FVAFSPPVFKRIDFATEYKVRLLTSVEPFTETIGGSSFTTLTKKPRHYVGSDLDFMVS
                     DALGISLKYRFGSQPPAFKFVDHSASVGLTFKLKQANK"
BASE COUNT          241 a          274 c          288 g          251 t
ORIGIN      
        1 cgaggattgc gggctcttcg tcgatgtggt cgggcgggat cagcatcgtc accggttttc
       61 cgatcgcctc gtccgcggtg taaccgaaga tacgctgggc gccatggttc caactcgtaa
      121 tgatgccgtc gagcgttttg ctgataatcg cgtcttcagc cgactcgatg agcgcagcca
      181 gccagtaagg cgccagctca gggctcttta aatctgcaac ttgatccgtc ttgaatgcgt
      241 cgtgcggagc cgcatcgtct ggagcttgac cgggcataac caattgatct ctgcgctgac
      301 ttctcggagg agagtgtttt accgggggga caaacagggg taggcggatt atagcagttc
      361 aaacgtgcgg cacctaaaca ctcaaatcta tacccgtgtt tccgctgacg gataggtcag
      421 gggccgacgg atgacgaccc cttgacgcga agccttcatt tgttcgcttg tttcaatttg
      481 aaagttaagc ccactgaagc gctgtgatcg acgaacttga acgccggcgg ctgcgagcca
      541 aacctgtatt tcagactaat gcccagcgca tccgaaacca tgaaatcgag atccgagccg
      601 acatagtgtc ttggtttctt agtcagcgtc gtgaacgacg atccaccgat ggtttcagtg
      661 aatggttcaa ctgaggtcag caaccgcacc ttgtactcgg tagcaaaatc gatgcgctta
      721 aacaccggtg gactgaacgc gacgaaatac gcgttagcgc cgaatttcca gcgccaaaga
      781 ttcccgagtc catcattcgg gttcaaagcg tgcttgaagt tatggcctgc ttcgaatccc
      841 gcggtgaaat ccacagcagc aatattgttt tcgttgaaac gatgcggagg cagaacaagg
      901 attccgtgaa ggtccgtcat gatgttgcga ttcttatttc tcttgtcgaa ctcgaaaccg
      961 aatgcatcag aacggaggat cagccccctt gtccgaccga acacgaagat cttttcatac
     1021 gtgagcgcgg tcttgatgct gtccgggtcg atgt
//
LOCUS       DASTWE010001084         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1095515, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001084 DASTWE010000000
VERSION     DASTWE010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095515"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1728)
                     /locus_tag="VFT30_11275"
     CDS             complement(<1..>1728)
                     /locus_tag="VFT30_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU5093259.1"
                     /translation="RKEPAKEQIFLYKGGIVSFVEHLNEAKTPLHKPIYVKVEKPEMI
                     LEVALQYNDSYAENLFSFANNINTKEGGTHLIGFKAALTRTINNYANANDLFKKETES
                     LTGDDVREGLTAVVSVKVRNPQFEGQTKAKLGNSEVKGVVEAAVNEALGNYFEENPPV
                     ARKIIGKAVDAARAREAARKAKDLIRRKSALDGGSLPGKLADCSEKDPAMSELYIVEG
                     DSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSDEIRTLIMALGTGIGRKRE
                     EGDKSEKDTFDIAKARYHKIILMTDADVDGSHIRTLLLTFFFRQMPELLERGYIYIAQ
                     PPLFKVKKGKTERYLKDEGSLNEYLADLAVEDVELYTEGAQGYVTGRRLLPILKKMIS
                     FETLLARLNKKPHEAAMLRAFVDEPGLDRELLKDREALNRVVVGVKKALLLAFPKLEP
                     TFDIFADEEHQSNKVTCRLHVNGMVHSIEINHDLVGSADFREVQKLAPSVIGLGRAPY
                     RLNTKGQERQLVTTAETVKTILELGKQGLGIQRYKGLGEMNPSQLWETTMDPEKRTLL
                     KVQLEDVTGV"
BASE COUNT          335 a          499 c          434 g          460 t
ORIGIN      
        1 acgccggtga cgtcctccag ctgaaccttc aagagcgtgc gtttctcggg atccatcgtc
       61 gtttcccaca attgggatgg attcatttca ccaagtcctt tgtaccgctg aataccgaga
      121 ccttgctttc cgagttcgag gatcgttttc accgtttcag ctgtcgtaac tagctgccgt
      181 tcctggccct ttgtgttcag cctgtacgga gcccgaccca gtccaatgac ggacggcgca
      241 agcttttgga cctcccgaaa atcggccgat cccactaagt catggttgat ttcgatgcta
      301 tgcaccatgc cgttgacatg aagccgacac gtgactttgt tggactgatg ttcttcatcc
      361 gcaaagatat cgaatgtcgg ttctaacttc gggaatgcaa ggaggagtgc cttcttgacc
      421 ccaaccacca cgcgattgag tgcctctcgg tccttgagga gctcgcgatc gagccctggt
      481 tcatcgacaa aagcgcgcag catggctgct tcatgcggct ttttattgag gcgagcgagc
      541 aacgtttcga acgagatcat cttctttagg atcggcaaca gccgacgccc tgtcacatat
      601 ccctgggcac cttccgtgta cagttcgacg tcctcaaccg ccaaatcggc caagtactcg
      661 ttcaacgacc cctcatcctt gagataccgc tccgttttgc ctttttttac tttgaacagc
      721 ggtggctgag cgatgtagat atagccccgc tcgagcaatt caggcatttg ccggaagaag
      781 aacgtcaaga gcagggtgcg gatgtgactc ccgtcgacgt cggcatcggt catgaggatg
      841 atcttgtgat agcgggcttt tgcgatatcg aacgtatctt tctccgattt gtcgccttct
      901 tcacgcttgc gaccgatacc agtccccagc gccatgatga gtgtcctaat ctcatcactg
      961 gaaagcatct tgtcgaagcg tgccttctcg acattcaaga tcttcccttt cagggggagg
     1021 atcgcttgga acttgcggtc acgtccttgc ttcgcagatc cgccggccga atctccctca
     1081 acgatgtaga gttcgctcat ggccggatct ttttccgaac aatcagccaa cttgccggga
     1141 agcgagccgc catcgagcgc gctcttacgc cggatcagat ccttggcttt ccgagcagct
     1201 tcacgtgcac gggctgcatc gacggccttg ccgatgatct ttcgagcgac gggaggattt
     1261 tcctcgaaat aattgcccaa ggcctcgttg accgcggcct cgacaactcc tttcacttcg
     1321 ctgttcccga gcttggcttt cgtctggccc tcgaactgcg gattgcggac tttgacgctg
     1381 acgactgccg tcaggccctc ccgtacgtca tcgccggtca gcgattccgt ctccttctta
     1441 aagagatcgt tggcattagc gtaattattg atcgttcttg taagggcggc tttgaaccca
     1501 atcaagtgcg tcccgccttc tttggtattg atgttgttcg cgaacgagaa caggttctcc
     1561 gcgtagctgt cattgtattg gagcgccact tccagaatca tttccggctt ctcaaccttg
     1621 acgtagatcg gcttgtgcaa cggcgttttg gcttcgttca gatgttcaac gaaggacacg
     1681 atcccgccct tgtacaggaa gatctgttct ttcgccggct ctttccgt
//
LOCUS       DASTWE010001085         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_384205, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001085 DASTWE010000000
VERSION     DASTWE010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384205"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VFT30_11280"
     CDS             complement(<1..1156)
                     /locus_tag="VFT30_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5093260.1"
                     /translation="MREVILWTTFCVCFSSLLAACGGESGTQDIAPASTTLKLQTITR
                     NLNSPVFLTAPPGDPNRLFVLEQGGAIKVLDRATGNRLSIFLTLTGITSGGERGLLGL
                     AFDPNYNANGRFYLHYTDANGAITIARFSVSSTDANIADPASQVILVSIPHPTFANHN
                     GGMLAFGPDGCLYAAVGDGGSSGDPNNNAQNLASRLGKLLRIDPTTGTACTNGTLNPF
                     VLSGGDQLIWNYGLRNPWRFSFDGDDLYIGDVGQGAREEINVSQGPNAGRGLNYGWRL
                     MEGSTCFNPATNCNNGTLTLPIVEYTHDNGACSVTGGYVYRGQAAPAIQGTYFYADFC
                     AGFVRSFRFNNGSALEQKEWPLLAAPSITSFGQDGLGELYITSQGGTVSRI"
BASE COUNT          280 a          302 c          325 g          279 t
ORIGIN      
        1 cgatccgaga cacggtgcca ccctgagaag tgatgtaaag ttctccaagg ccatcttgac
       61 cgaagcttgt gatcgacgga gcggcgagca gcggccattc tttttgttcg agagctgagc
      121 cattattgaa gcggaaactt cggacaaatc ccgcgcagaa atctgcatag aaataggtcc
      181 cttggatcgc tggtgcggct tggcctcgat aaacataccc tcctgttacg gagcaggctc
      241 cgttgtcatg cgtatattca acaatcggca atgtgagtgt tccattattg cagttggtgg
      301 ctggattaaa acaggttgaa ccttccataa gccgccagcc gtaattcaat ccgcgtcccg
      361 cattggggcc ttgtgacaca ttgatctcct ctcgggcacc ttggccgacg tcaccgatat
      421 acagatcgtc tccatcaaac gagaagcgcc agggattacg gagtccataa ttccagatca
      481 actgatcgcc tccgctgagg acaaaagggt tgagcgttcc attggtgcag gctgttccgg
      541 tggtcggatc gatccggaga agttttccaa gtcggcttgc gagattctgg gcattgttgt
      601 tcggatcccc tgagcttccc ccatctccaa cggctgcgta cagacaacca tccggtccga
      661 acgccaacat gcctccgtta tggttagcga aggttggatg gggaatggaa accaagatga
      721 cctgcgatgc aggatcagcg atattggcat cagttgacga caccgaaaat cgagcgatag
      781 tgatcgcgcc attggcgtcg gtatagtgga ggtagaatcg tccgttggca ttatagtttg
      841 ggtcaaaggc cagacccaac aatcctcgtt ctcctccgct cgtgatgccg gtcagtgtca
      901 gaaatatcga gagcctgttc ccagttgccc gatcaagaac tttaatggcg cctccctgtt
      961 ccaggacaaa caggcgattc ggatccccag ggggagcagt caggaatacc ggagagttaa
     1021 ggtttctcgt gatggtttgg agcttcagag tagtcgaagc tggggcgatg tcctgggtgc
     1081 cactttctcc tccgcatgca gcaaggaggc tggagaaaca cacacagaaa gtggtccaca
     1141 gaatgacctc tctcatgagt aagtctttgt ccttattgag ggatac
//
LOCUS       DASTWE010001086         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1063515, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001086 DASTWE010000000
VERSION     DASTWE010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063515"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFT30_11285"
     CDS             <1..370
                     /locus_tag="VFT30_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093261.1"
                     /translation="SIGLPGTNGFVGEFLILLGAFKANWWFGAIAVLGVIFGAAYFLW
                     YYERSMLGPLGAAVKGTMSDLQPREKIIAVSLSVMILWIGLYPSPFLRIMNGSVQALV
                     DRVSQETQVSLEGNRFGRGY"
     gene            379..>1747
                     /locus_tag="VFT30_11290"
     CDS             379..>1747
                     /locus_tag="VFT30_11290"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5093262.1"
                     /translation="MGEYALLYILFAPFAGALMLIFVSNRQPLLVRSIAASAAFVSLV
                     ASLYIFYAYDPVKGGFQFVQKFEWSRQLGISLYLGVDGIGTPLVLASSILLFAGIFVS
                     WHIKDRTKEFYIWLLILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDPEGLKVRDSVGFILNFGSNSKEYAAMKLVLFLSAFAVVALMGILLIYKYSGLN
                     TFDILVLREQANFMNIPVLGTTLDKIIWVLIFFGFASIAPLWPLHSWSPVGHAAAPAA
                     TSMLHAGVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCMFSIIYGGFVAFYAKDTK
                     YVIGYSSSSHMGYVFLGMAALNYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHSRD
                     IPSLGGLSNKMPFISACFIVACMASIGMPGTVNFIAEIMIILGSWNNYPVQVIVAMLG
                     IVLTLA"
BASE COUNT          330 a          445 c          466 g          506 t
ORIGIN      
        1 gtcgatagga cttccaggca ccaatggctt tgtcggcgag ttcctcattc tcttgggcgc
       61 gttcaaagcc aactggtggt tcggagctat cgccgttctg ggagtgattt tcggcgctgc
      121 gtattttttg tggtactacg aacgttcgat gttaggtccg ttgggtgctg ccgtgaaagg
      181 aacgatgagc gatctgcaac cgcgcgagaa gatcattgcg gtgtcgctgt cagtcatgat
      241 tctctggatc gggctttatc cctcaccgtt cttacgaatc atgaacggat cggtgcaagc
      301 actcgttgat cgagtctctc aagagaccca ggtgtcatta gaaggcaacc gcttcggtag
      361 agggtattag gtcgcccgat gggagagtac gccctgctct atatcctttt tgccccgttt
      421 gccggggctc tcatgttgat ttttgtttcc aatcggcagc cgctgctcgt gcgaagtatc
      481 gcagcaagtg ccgcatttgt gtcgctggtt gcatcactct acatctttta tgcgtacgat
      541 ccggtgaaag gcggcttcca gtttgtccaa aagtttgagt ggtccaggca gctcggcatt
      601 tcattgtatc ttggcgtaga tggaatcggg actcctctcg tgctcgcctc ttccattttg
      661 ttgtttgccg gcatcttcgt ctcttggcac atcaaggacc ggacgaagga gttttacatc
      721 tggctgctga tcttggcggc cgccacgatc ggcgtgttca tgtctctcga cctgttcttc
      781 ctctacttct tctatgagat gtccgtgatt ccgatgtatt tgttgttggg aatgtggggc
      841 agccacacaa agaagtattt ggagatgacc gatcccgaag gtctaaaagt gcgggactca
      901 gtcggtttta tcctcaactt tggatcgaac agcaaagaat atgcagcgat gaagcttgtg
      961 ctcttcctgt ctgcgtttgc cgtcgtcgcg ctcatgggta tcttactcat ttataaatat
     1021 tccggcctga acaccttcga tatcctggta ctccgtgagc aggccaattt catgaatatc
     1081 ccggtgctcg gcacaacgtt ggacaagatc atctgggtgt tgatcttctt cggatttgcg
     1141 tcgatcgccc cgctgtggcc gctgcattct tggtctccgg tcggccatgc ggctgcgccg
     1201 gccgcaacca gcatgcttca tgcgggtgtc ttgatgaagc tcgggcattt ttccattatt
     1261 cgggtggcct ttgaaattct ccctgaaacg accagagaac tgatgccgat agccgccgtt
     1321 ctgtgcatgt tcagcatcat ctacggcggc ttcgttgcgt tttacgcaaa agacaccaaa
     1381 tacgtgatcg gatactcaag ttccagtcat atgggttatg tgttcctcgg catggcggcc
     1441 ttgaattaca tcagcttgag cggtgccgtc atctacatgt tcgcccacgc catggcgacg
     1501 ggcatgctct ttgccatggc aggatgggtc tatgaccaaa cccattcacg cgacatcccc
     1561 tcgttgggtg gtctttcgaa caagatgccg ttcatctccg cttgtttcat cgtagcctgc
     1621 atggcttcga tcgggatgcc cggtacggtg aattttatcg cggaaatcat gatcatctta
     1681 ggcagttgga acaattaccc agttcaagta attgtcgcca tgttgggcat cgtgctgacc
     1741 ttggcgt
//
LOCUS       DASTWE010001087         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_546473, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001087 DASTWE010000000
VERSION     DASTWE010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546473"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFT30_11295"
     CDS             complement(<1..713)
                     /locus_tag="VFT30_11295"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEU5093263.1"
                     /translation="MKVTRGYSDEAVRPYPVGTIGNFDGHHLGHHALLKMVVDTARRA
                     HGTAVVLTFAPHPVKILAPHVDLRFLTSGEEKRARFEQAGIDEVVSLEFTQEFASLSP
                     EMFAEQVLSRGLGLKEIFVGQHFAFGHKRAGRIGDLAALGKRFGFIVHPIPPVMIDGG
                     VVSSTKIRQLIMIGQVDQAAVLLGRHYALSGVVSSGEGRGRTLGCPTANLRLPPDRVA
                     PADGIYASVSILDQERHDSV"
     gene            complement(720..1691)
                     /gene="hemB"
                     /locus_tag="VFT30_11300"
     CDS             complement(720..1691)
                     /gene="hemB"
                     /locus_tag="VFT30_11300"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU5093264.1"
                     /translation="MGFPIQRLRRLRQHESLRRMVRETTLSPSDFIYPLFVVEGQDRR
                     EEIASMPGQFRLSVDLLVKEAGEIQALGIPAIMLFGIPAHKDDRGSSGSDPNGIVQRA
                     IRAVKQQVPGLLVITDVCIDEYTSHGHCGIVKDGKILNDETLECLTVMARTHAEAGAD
                     MVAPSDMMDGRVAAIRRELDRAGFSDLPILAYAAKFSSCFYAPFRDAAFSSPQFGDRQ
                     SYQMDPANAREALREIELDIDEGADIVMVKPALPYLDIIAMARAHTLLPLAAYQVSGE
                     YSMIKAAARAGWLDESRAMMESLLAIKRAGADLILSYFAKDAVKLLH"
     gene            complement(1700..2092)
                     /locus_tag="VFT30_11305"
     CDS             complement(1700..2092)
                     /locus_tag="VFT30_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5093265.1"
                     /translation="MVPVKSFMIPREKFVTVPRDTDARTAARIMRDRGIGSLFVTNDR
                     EIIGIITDTDMVRRVVAAGADAIKTTVEQIMSAPIMTIEESKTLLDANDLMAQSHLRH
                     LGVTRDGKLVGVISVRDLVVFLTNLPRK"
     gene            complement(2146..>2533)
                     /locus_tag="VFT30_11310"
     CDS             complement(2146..>2533)
                     /locus_tag="VFT30_11310"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU5093266.1"
                     /translation="EVARDLLPDELRAAGAHVAVVAVYRTLTPDHHFEEWRQELMNRR
                     IHVVTFTSSSTVRNFVAMLGGVEAVKPLLQSVLIACIGPITAKTAEEYGLTVSIMPSE
                     NTIPALVDAIAHHYGSCEQVTMGALQ"
BASE COUNT          569 a          753 c          665 g          546 t
ORIGIN      
        1 accgaatcat gtctctcctg atccagaatc gagacagatg cataaattcc gtcggcaggt
       61 gccacacggt ctggtggtag ccgaaggttc gccgtagggc atcctagtgt tcgccccctc
      121 ccctccccgg aagatacgac tccgctcagg gcatagtgtc ggccgagcaa gacagccgct
      181 tgatcgacct gcccgatcat aatgagctgc ctgattttcg tcgaactgac cactccaccg
      241 tcgatcatga cgggaggaat cggatggacg atgaagccga atcgtttgcc gagcgcggcc
      301 agatcgccaa tccgtcctgc tcgcttgtgc ccaaaggcaa aatgttgtcc gacaaagatc
      361 tctttgagtc cgagccctct agacagaacc tgctcagcga acatctctgg agaaagtgat
      421 gcaaattctt gagtgaactc aagggagacc acctcatcga tgccggcttg ttcgaaccgg
      481 gcacgttttt cttccccgct cgtcaggaac cgcagatcaa cgtgaggcgc aaggattttc
      541 accgggtgag gggcgaacgt caggacgaca gccgttccat gggcacggcg cgccgtgtca
      601 accaccattt ttaagagcgc gtgatgaccg agatgatgac cgtcgaaatt cccgatggtt
      661 cccaccggat acggccgtac ggcctcgtcc gaatatccgc gggtcacttt catcgtcgat
      721 caatgaagca gcttgacggc gtctttcgca aagtacgaca ggatcagatc ggctcccgct
      781 cgcttgatcg ccaagagcga ttccatcatg gcacgcgatt catcgagcca ccctgctctg
      841 gccgccgcct taatcatact gtactcgccg ctcacctgat atgccgctaa cgggagaagc
      901 gtatgagcac gggccatggc aatgatgtcc agataaggta gtgccggttt gaccatcacg
      961 atatcggcac cttcgtcgat atcgagctcg atctcgcgca gcgcttcgcg cgcgttagcc
     1021 ggatccatct gataagactg tcggtcaccg aattgagggc tggaaaaggc cgcgtcgcga
     1081 aaaggggcat agaaacagga ggagaatttg gcggcatagg ccaggatcgg aagatcggaa
     1141 aatccggcgc gatccagctc gcgtctgatt gcggcgacac gaccatccat catatccgat
     1201 ggcgcgacca tatcggctcc agcctcggca tgggtgcgtg ccattaccgt aagacactcg
     1261 agcgtctcat cgttgagaat ctttccatcc ttgacgattc cgcagtgccc gtggctcgta
     1321 tactcgtcga tacagacatc cgtgatcacg agtaatcccg gcacttgttg tttgacggct
     1381 ctgatcgccc gctgcacgat accgttcgga tccgaccctg aactaccacg atcgtctttg
     1441 tgcgccggta taccaaatag catgatggcc ggaataccta aggcttgaat ctctcccgcc
     1501 tctttgacca gcaaatcgac agagagccga aattggcccg gcatcgaagc gatctcttca
     1561 cgacgatcct gcccctcgac cacaaacagc gggtagatga aatccgacgg ggacagcgtc
     1621 gtttcacgca ccatccgacg caacgattcg tgttgcctca atcgtcggag ccgctggatt
     1681 ggaaacccca tggctcacct tactttcgtg gcaggttcgt caagaacacg acgaggtcgc
     1741 gaacggaaat aacccccacc agcttcccat cgcgcgtgac accgagatgg cggagatggg
     1801 attgcgccat caaatcgttg gcatcgagta acgtcttgct ttcctcaatg gtcatgatcg
     1861 gcgccgacat gatttgctca accgtcgtct tgattgcatc cgccccggcc gcaactactc
     1921 gacgcaccat atccgtatcc gtgatgatgc cgataatctc acgatcgttg gtcacgaaca
     1981 aactgccgat ccctcgatcg cgcatgatgc gggcggctgt ccgtgcatct gtatcacgag
     2041 gcaccgtcac gaacttttct cggggaatca tgaatgactt tacaggaacc atctcatcat
     2101 cctcctttgg taactatcta taatgataca tctcgcgccc tttcgttact gcagagctcc
     2161 catggtaact tgctcacagc ttccataatg gtgggcgatc gcatccacca gcgcgggaat
     2221 cgtattctca ctcggcatga tcgagaccgt caaaccatat tcctctgccg tcttggctgt
     2281 gatgggtccg atgcaggcga tcaagacgga ttgcaagagc ggtttcaccg cttctacacc
     2341 accgagcatc gcgacaaagt tgcggaccgt ggaagagctg gtaaacgtga cgacatgaat
     2401 ccgacgattc atgagttcct gccgccactc ctcgaaatga tggtctggtg tgagcgttcg
     2461 atacacggcc acgacggcga cgtgtgcgcc ggctgcacga agttcgtccg gcaacagatc
     2521 tctggccact tcg
//
LOCUS       DASTWE010001088         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_740024, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001088 DASTWE010000000
VERSION     DASTWE010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740024"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFT30_11315"
     CDS             complement(<1..714)
                     /locus_tag="VFT30_11315"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEU5093267.1"
                     /translation="MSRCEVQQVKCPSCGASQDAKVFVSVNGARIKSAADRLIDGSWG
                     VLSCLKCGADYHRPMPRLFTDLPGKLWLVRYDDSERDRYPMREEEANTIFEREWLERP
                     PAAIRDQALSMQRRICFGPAQLREKLLLRRHGLDDRMVECLKLVLMREYVSDLFTFGP
                     AEFYVHQVEPGGLGMVAVSIGEKHQAFELKVEQPTLEEIAQDAERFREAFPELFTNLY
                     VSASRYVTESETEKVSHSIR"
     gene            complement(711..>1184)
                     /locus_tag="VFT30_11320"
     CDS             complement(711..>1184)
                     /locus_tag="VFT30_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093268.1"
                     /translation="REMGSLLGKDTVHFVAFTARPHSPIWHKFYFSQYLTPENFDHAA
                     SRLQRVVKRFAPDTQSASRWAIYHDRLARRHCTDTLFVSLALTEDECDRSVKIDYPGV
                     SPLVAAGLLDGPSSERAHERFQRLCAVAGRPTLSYLGVRMGVEEQPTLKGYADLP"
BASE COUNT          267 a          362 c          312 g          243 t
ORIGIN      
        1 tcgaatcgag tgggaaacct tctccgtttc cgactccgtc acgtaacgcg aagcgctcac
       61 atagagattg gtaaacaatt ccgggaaggc ctcgcggaac cgttcagcat cttgggcgat
      121 ttcttcgaga gtcggctgct caaccttgag ctcaaaggcc tgatgcttct ctccgatcga
      181 cacagccacc atgccgaggc cgcccggctc gacctgatgc acgtagaact ccgccggccc
      241 gaacgtgaac aagtcactca catattcacg catcagaaca agtttcaaac actccaccat
      301 ccgatcgtcg aggccgtggc ggcgcaataa caatttctcc cgtagttgtg caggtccaaa
      361 acaaatgcgg cgttgcatgg acagtgcttg gtctcggatc gcagccggag gccgctcaag
      421 ccactcccgt tcaaaaatcg tgttcgcctc ttcctctctc atggggtagc ggtctcgctc
      481 tgagtcgtcg taacgaacga gccacaactt tcctggcagg tcggtaaaca ggcgaggcat
      541 cggccgatga taatcggcac cacactttag acaagacaac actccccaac ttccatcaat
      601 gagccggtct gccgcgctct tgatgcgggc gccgttgaca ctgacgaaga ctttcgcgtc
      661 ttgactcgct ccgcacgaag gacacttcac ctgttgaact tcgcatcggc tcatggcaag
      721 tctgcgtagc ctttcagggt cggttgttcc tctactccca tccgcacccc caggtacgag
      781 agtgtcggtc gtccagcgac agcgcagagc cgctggaatc gctcgtgagc ccgttcggat
      841 gacggtccat cgagcaaccc cgccgctacg aggggtgaca ctcccggata gtcgattttc
      901 accgatctgt cacactcgtc ttcagtcagg gccagagaca cgaacagggt gtctgtgcaa
      961 tgacgccgag ccaatcggtc atgatagatg gcccaacggg aggccgactg cgtatcagga
     1021 gcgaaccgct tcacaacccg ttgcaaccgc gatgcggcat gatcgaagtt ttcaggcgtg
     1081 agatattgag agaaataaaa cttatgccag atagggctgt gcggcctcgc cgtgaaggcg
     1141 acgaaatgca ccgtgtcctt gcccagaaga gaacccattt cccg
//
LOCUS       DASTWE010001089         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_708003, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001089 DASTWE010000000
VERSION     DASTWE010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708003"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFT30_11325"
     CDS             <1..400
                     /locus_tag="VFT30_11325"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEU5093269.1"
                     /translation="DIAAIYIPDVIKVDLSTQGVRFAGGGYDHPTNPDDEGFSRLSIF
                     KGDLLQSNSQDPFKNGGLIAGGWPNGRRFGDDVVDIAVIALINDLRNPPFDLKKIIIA
                     GDGVDKNDIPFNKVFPYEATPLNGRNHSHP"
     gene            522..1097
                     /locus_tag="VFT30_11330"
     CDS             522..1097
                     /locus_tag="VFT30_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5093270.1"
                     /translation="MTEFNIHTVESAPQGSKPALEQLKKHVGFIPNLAATMAESPNLL
                     EAFTSLRAISGRGTFTPVEREAVALAVSFDNNCTYCMAAHSTFAKMNGIPEDDLSLLR
                     VGKSPSSPRLQALSALAHQVVRSKGHIAPEDIQGFLNVGFSQAQLLEVLVGISTTAIA
                     NYMHNIAKTPIDEAFQAQSWTPSSSGARSSV"
BASE COUNT          276 a          312 c          293 g          267 t
ORIGIN      
        1 cgatatcgct gccatataca tccccgatgt gatcaaggtt gatctatcaa cgcagggtgt
       61 gcgtttcgcg ggcggcggtt atgatcatcc gactaatcct gacgatgagg gtttctcgcg
      121 actgagcatc tttaagggcg atttactgca aagcaacagt caggacccgt tcaagaacgg
      181 cggtttgatt gccggtggtt ggcccaacgg caggcgtttc ggcgacgacg ttgtggacat
      241 tgccgtcatc gccttgatca acgacttgcg caatccaccg ttcgatctga agaagatcat
      301 aatcgctggt gacggcgtag ataagaacga cattccgttt aacaaagtgt tcccgtatga
      361 agcgacaccg ctgaacgggc gcaaccacag tcacccataa ccaggcatcg ggttcacttc
      421 cctcaactct gtttctggaa cggacgccat cgtgcgctcc tagcgggaca gtgtgtctgc
      481 ggggagaaat acaggacatt agcaagaaaa ggaaaactat tatgaccgag ttcaatatcc
      541 acacagtcga atctgctcct cagggttcaa agccggccct ggagcaactc aagaagcatg
      601 tcggattcat acctaacctt gcagcaacta tggctgaatc ccccaacttg cttgaggcgt
      661 tcacatctct tagagcgatc tccgggcgtg gaacattcac tccggttgaa cgcgaagcgg
      721 tcgctctggc ggtcagtttt gacaacaact gcacgtactg catggcggcc cattcaacat
      781 ttgccaagat gaatggtatt ccagaggatg atctgagcct gcttcgggtg ggcaagtcac
      841 cttcttcccc acgcctgcaa gctctcagcg ccttggcgca tcaagtcgtt cgcagcaagg
      901 gacacatagc gcctgaggat attcaaggat tcctcaacgt aggtttctct caagctcagc
      961 ttttggaagt tctggttggg atcagtacca cagcgattgc caattacatg cacaacatcg
     1021 ccaaaacgcc tatcgatgaa gcgttccagg cccaatcttg gactccttca tcctcaggag
     1081 cccgtagctc agtgtagacg gctcttgggc cagggtgaat accgacagtc tatcaactgc
     1141 cggttttc
//
LOCUS       DASTWE010001090         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1224612, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001090 DASTWE010000000
VERSION     DASTWE010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224612"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="modA"
                     /locus_tag="VFT30_11335"
     CDS             <1..307
                     /gene="modA"
                     /locus_tag="VFT30_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5093271.1"
                     /translation="LELWEGIQSQIVMGESLGQTMGFIESGNAQLGFVALSQVMDPKI
                     KGKGSRWDVPNNLHEPIQQDVILLTKGKNNPAAKALMEFIGSPQAKAIIERYGYELK"
     gene            317..991
                     /gene="modB"
                     /locus_tag="VFT30_11340"
     CDS             317..991
                     /gene="modB"
                     /locus_tag="VFT30_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEU5093272.1"
                     /translation="MGALTDLDLSALWVSVRLATMTVIVLLIVGTPLAWWLAHTRVRW
                     RPIVEAAVALPIVLPPTVLGFYILITLGPYGPLGRVTNLSLAFTFTGLVIASVFYSMP
                     FVLQPLQGAFEAVGKAPLEAAWSLRASKWDAFLTVISPIALRGYISAIVLGFAHTMGE
                     FGVVLMVGGSIPGQTRVLSTTIFEHVEMMEYDQAHTIAAILLIFSFIVLLAVYIANRR
                     FPIHVS"
     gene            988..>1706
                     /gene="modC"
                     /locus_tag="VFT30_11345"
     CDS             988..>1706
                     /gene="modC"
                     /locus_tag="VFT30_11345"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEU5093273.1"
                     /translation="MSHLLARFDVRFPTFHLNVDVDIPMSGITAIFGPSGSGKTTLLR
                     CLAGLERAPNGFMQFGNDIWQDEKIDLCLPLYKRPIGYVFQEPRLFPHYNVRANLLYG
                     YKRVPVEERRIGMEQVVEILGIGHLLERRIHKLSGGEQQRVAIGRALLTSPKLLLLDE
                     PLASLDVQRKQELLPFMRRLHEELHIPVMYVSHAISEILQLADRVLLLKEGKVIAAGI
                     LNEMFTSLQFRGNFGAHRIGA"
BASE COUNT          382 a          463 c          450 g          411 t
ORIGIN      
        1 gctggaactc tgggaaggca tacaatccca aattgtcatg ggggaaagtc taggacaaac
       61 catggggttt atcgagtcag gcaatgctca gttggggttc gtggccttgt ctcaagtcat
      121 ggaccccaag atcaagggaa agggcagtcg ctgggacgtc cccaacaatc tgcacgaacc
      181 aatccaacag gacgtgatct tactaacgaa gggaaagaac aacccggcgg caaaagcact
      241 catggagttc attggcagcc cgcaggccaa ggcgatcatc gaacgctacg gttatgagtt
      301 gaagtagaga gtacgcatgg gagctctgac agatctggat ctaagcgcgc tctgggtcag
      361 cgtgcgactg gcaacgatga ctgtgatcgt gctgctgatc gtggggacgc ctcttgcctg
      421 gtggctggcg catacccgag tcagatggag acctatcgta gaagctgcgg tcgcactacc
      481 gatcgtgctt ccacccaccg tcctcgggtt ctacatcctt attacactcg gcccatacgg
      541 accattgggc cgtgttacaa acctctcgct cgccttcact ttcaccggcc tggtgatcgc
      601 ttcggtcttt tactccatgc cgttcgtgtt acagcctttg caaggtgcat ttgaagccgt
      661 cggcaaggct ccgctggaag cagcctggtc acttcgcgcg tcaaagtggg atgcatttct
      721 gacggtcatc tccccgattg cactgcgagg ctatattagt gccatcgtgc tcggattcgc
      781 gcatacgatg ggagagtttg gggtggtgct catggtcggt ggatcaattc ccggacaaac
      841 gcgtgtcctt tccacgacta tctttgagca tgtggaaatg atggaatacg atcaagcaca
      901 tacaattgca gccatactcc tgatcttttc ttttatcgtg ttgttggctg tctatattgc
      961 caaccgacga tttcccatcc acgtgtcatg agtcatttgc tggcacgctt tgatgtgcga
     1021 tttccgacct ttcatctgaa cgtcgatgta gatatcccaa tgtccggtat taccgccatc
     1081 ttcggacctt ccggatcggg aaagacaacg ctgttgcgat gcctggcggg acttgagcga
     1141 gctcccaacg gtttcatgca gttcggcaac gatatctggc aggacgagaa aatcgacctg
     1201 tgtctcccgc tctataaacg gccaatcgga tatgtgtttc aagaacctcg cttattcccc
     1261 cattacaacg tgcgcgcaaa tcttctgtac ggctacaaac gcgtgccggt ggaagaacgc
     1321 cgcatcggca tggaacaggt cgtcgagatt cttggaatcg gccatctctt agagcgccgc
     1381 attcataaac tatccggcgg agagcaacag cgggtagcga tcggccgcgc gctactgacg
     1441 agtcccaaac tactactcct ggatgaaccg ctcgcttcgc tcgacgttca acgcaagcaa
     1501 gagctccttc ctttcatgcg tcgtcttcat gaggagctgc atatcccggt aatgtacgtc
     1561 agccatgcga tctccgaaat cctgcaactc gcagatcgtg tccttctttt gaaggaagga
     1621 aaggtcatcg cggccggcat actcaatgag atgttcactt ccttacaatt ccggggaaat
     1681 ttcggcgcgc atcgaatcgg cgcaat
//
LOCUS       DASTWE010001091         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_416867, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001091 DASTWE010000000
VERSION     DASTWE010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416867"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFT30_11350"
     CDS             complement(<1..256)
                     /locus_tag="VFT30_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU5093274.1"
                     /translation="MGSKLYVGGLPYSATEQQLNDLFGAHGSVASVRIIADKFTGQSR
                     GFGFVEMSSDAEAKAAITALNGTDMGGRTLTVNEAKPMEQR"
     gene            complement(509..586)
                     /locus_tag="VFT30_11355"
     tRNA            complement(509..586)
                     /locus_tag="VFT30_11355"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Glu,seq:ttc)
     gene            complement(727..1281)
                     /gene="rfbC"
                     /locus_tag="VFT30_11360"
     CDS             complement(727..1281)
                     /gene="rfbC"
                     /locus_tag="VFT30_11360"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEU5093275.1"
                     /translation="MTPIDIPDVLLLEPSVLSDHRGWFAETYHELRYCEAGITEHFVQ
                     DNCSRSVRNTLRGLHFQEPHAQGKLVMALEGTVYDVVVDIRKGSPTFGKWYGIELSEK
                     NLYQMYIPPGCAHGFCVTSETATFLYKCTAYYSPKDEHGILWNDPALGILWPISEPIL
                     SQKDQAYRTLKGMDMELPLYRAVR"
     gene            complement(1300..1725)
                     /locus_tag="VFT30_11365"
     CDS             complement(1300..1725)
                     /locus_tag="VFT30_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093276.1"
                     /translation="MPSLDVQNPEMPDLQFVLFVSALCTADLTTINISQELRHTIFDR
                     CWALLHTEAPPTNPKERVLDLRGGTELTLDACASTIRSLLLDANIATLVWDHPVSEAR
                     MTSTPEALPLIDRLEQLYPDHPTVVDPPRHPPRDSNPTS"
BASE COUNT          436 a          479 c          511 g          404 t
ORIGIN      
        1 agcgctgctc catcggcttc gcttcattga ccgttaacgt gcggccaccc atgtccgtgc
       61 cattgagcgc cgtgatcgcg gctttcgcct cagcatccga ggacatctcc acgaagccaa
      121 atccacgcga ctgtcccgtg aacttatctg cgatgatcct cacagaggcc accgagccat
      181 gcgccccaaa caaatcgttc aactgctgtt cggtcgccga ataaggcaac ccaccgacgt
      241 aaagcttcga acccatgttg ggttcctcct tataatgatg acattgtctt gaacccgagg
      301 aacgggggaa ggagcgggcc gaagacgcag gcaatgcggc gacttaggac tgacttccga
      361 caggtttccc ggacaaggca atatcgatgt tgcgagcctt cctcaaacca gcttcgtcgc
      421 gaggcccgta cgacggcgaa acaaccgtaa cacaggggta ggtggtttac aacgtgtgtg
      481 cgtggcgatt gatctaggca tttgtatctg gtgtccccaa cgggatttga acccgtgtta
      541 ctgccttgaa agggcaatgt cctaggccag gctagacgat ggggacgtgc cacctacggg
      601 taagagaaca gcactcatac cacactcgta gctgccctgt cactaggccg gctcctcaga
      661 attccagcga tccgcggagc ctctttgtta cgtgcaagga atgagagtgg aaggaatgac
      721 aatttgctaa cgaacagctc tatagagcgg caactccata tccattcctt taagggtacg
      781 gtaggcttgg tctttctgtg agagaatagg ctcgctgatc ggccaaagaa tccccaacgc
      841 cggatcgttc cataagatgc catgttcatc tttcggcgaa taataagccg tgcacttata
      901 caggaacgtc gccgtctcgc tcgtgacaca aaagccatgg gcgcatcccg ggggaatgta
      961 catctgatac aggttcttct ccgatagctc gatcccgtac catttcccga acgtcggaga
     1021 ccccttccgg atatcgacga cgacgtcata tacagtccct tcaagggcca tgaccagttt
     1081 cccttgagcg tgcggttcct gaaaatgtag accgcgtaac gtgtttcgca ccgatcgtga
     1141 gcagttatct tggacgaagt gctcagtgat acccgcctca cagtagcgta gctcgtgata
     1201 cgtttccgcg aaccatcctc ggtgatcgga caagacagac ggttcgagca agagcacatc
     1261 aggaatgtca attggagtca cacgcacggc atcctctatt catgatgtcg gattggagtc
     1321 gcgcggtggg tgacgaggag gatcgacgac cgtaggatgg tcgggataca attgctccaa
     1381 tcgatcgatc aagggaaggg cttcaggggt gctggtcatg cgagcctcac tcaccggatg
     1441 gtcccatacc agagttgcga tgtttgcatc gaggagaagc gatcggatcg tagaagcgca
     1501 ggcatcgagc gtgagctccg ttccccctcg tagatcaaga acgcgttctt taggattcgt
     1561 gggtggtgcc tcggtgtgaa ggagcgccca gcagcgatca aagatcgtgt gtcgcagctc
     1621 ttgcgagata ttgattgtcg tcagatctgc cgtgcaaagc gccgacacga acaagacgaa
     1681 ttggaggtcg ggcatttctg gattttgaac atcgagagat ggcatagcta ctcattatca
     1741 ggagagaatg ccagtcgagt caactatcgt agaagggagc ggtttagaac ttgtggacgc
     1801 atgaggaatg acggatatgg tgactatcgt
//
LOCUS       DASTWE010001092         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_384463, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001092 DASTWE010000000
VERSION     DASTWE010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384463"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFT30_11370"
     CDS             <1..884
                     /locus_tag="VFT30_11370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5093277.1"
                     /translation="NLAEALLEAGDEIIIPIPYWVSYADQALLNDATPILLPTKEDEG
                     YTVHASELERLITPRTKAIIVNSPCNPTGATYDRTTLEQIASVAVRHDLLVISDEIYE
                     KVLYDGAAHISIASLGSEIAARTVIINGVSKSYAMTGWRIGYAAGPKDLVTAMANIQS
                     QSTSNPCSISQKAAVAALRFGEPFTRKMVEEFSRRRIAIVEGLNRIPGVSCRMPTGAF
                     YAFPNIKGVLGKRGPSGVLKTPNDVAQYLLHDVRIAVVPGEPFGSPEHLRLSYATNMT
                     NIIRGLDRMEQAFAKLG"
     gene            complement(1088..1291)
                     /locus_tag="VFT30_11375"
     CDS             complement(1088..1291)
                     /locus_tag="VFT30_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093278.1"
                     /translation="MEEEGVVVVLGVLVDELVGVAAGVDAGAGDAALGVVEAADSFFS
                     PFSTVLVPPVSPPALDGGLSLSE"
     gene            complement(1341..>1631)
                     /locus_tag="VFT30_11380"
     CDS             complement(1341..>1631)
                     /locus_tag="VFT30_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093279.1"
                     /translation="QLRSETQTLSIEKIQLQAQVTDLSKTKQELQGQIDELSRINREK
                     TGMITSLQNQLDLVRARALKSMNPKSPPHKALAKSTAKSAAKLPSKTSQRHR"
BASE COUNT          380 a          415 c          447 g          389 t
ORIGIN      
        1 acaatttggc agaagcctta ctcgaagccg gtgacgagat cattattccg atcccctatt
       61 gggtttccta tgcggatcag gcgcttctca atgatgcgac gccgattcta ttgccgacta
      121 aggaggacga gggttataca gtccatgcat cagagttgga acgactcatt acgccgagaa
      181 ccaaagccat catcgtgaac agcccctgca atcccaccgg tgcgacctat gatcggacga
      241 cattggaaca gattgcctca gtagcggtcc ggcacgatct ccttgtcatt tcggacgaga
      301 tttatgaaaa ggtgctctac gatggggccg cgcatatcag cattgcctcg ttgggatctg
      361 agatcgctgc tcgaaccgtc attatcaacg gcgtgtcaaa gtcttatgcc atgacaggat
      421 ggcgaatagg atacgccgct ggtccaaaag atctggtgac ggcgatggcc aatatccaga
      481 gccaaagtac ctcgaatccc tgctcgattt cacagaaggc cgccgtcgct gcgttgcgct
      541 ttggggagcc cttcactcgc aaaatggtgg aagaattttc ccgtcgccga atcgctattg
      601 tggaaggatt gaacaggatt cctggtgtgt catgtcgaat gccgactgga gctttctatg
      661 cgttcccaaa tatcaaaggg gtgttgggga aacgcggccc ctctggtgtg ctgaagacgc
      721 cgaatgacgt ggcgcagtat ctcttgcatg atgtccgaat cgcggtggtt ccgggtgaac
      781 cctttggaag cccggagcat ctccggctgt cctatgccac caatatgacg aatatcatcc
      841 gaggtcttga tcgaatggag caagcgttcg cgaagttagg gtaaggtagg attgactcaa
      901 agctaagagg gaggaaatca gtgccgatct acgaatatca gtgcgacagc tgttcgtatc
      961 gttttgaagt gaagcaaagc atcaaagatg atccgctttc gacgtgtgca cggtgtggga
     1021 aagcggtcag acggttgatt tcatcgcctg ggatcatgtt taaagggagt ggctggtacg
     1081 tcaccgacta ctccgataaa ctcaaacctc catccagcgc gggcggtgag accggcggaa
     1141 cgagtactgt agagaaagga gaaaagaagg agtcagccgc ttccaccaca cccagcgcag
     1201 catctccagc tcctgcatcg acgcctgcgg caacgccaac cagttcatcg acgagcacac
     1261 cgagtacgac aacaacaccc tcctcttcca aataagatcg aggtctgtcg tactcagact
     1321 actttcgaag cggtttctgt tcaccgatgc ctttgcgatg ttttggaagg cagcttggcg
     1381 gcggacttcg cagtcgattt cgccaaggcc ttatgaggcg gtgatttggg attcatcgac
     1441 ttcaacgctc tcgcgcgtac tagatccaac tgattttgga ggctggtgat cattcctgtt
     1501 ttttcccggt tgatgcgact cagttcatcg atctgcccct gtagctcctg tttggtcttg
     1561 gacaagtcgg tgacctgagc ctggagctgg atcttctcta tagacagagt ttgggtctcg
     1621 gaccgcaatt g
//
LOCUS       DASTWE010001093         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1483690, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001093 DASTWE010000000
VERSION     DASTWE010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483690"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="glnA"
                     /locus_tag="VFT30_11385"
     CDS             complement(<1..1020)
                     /gene="glnA"
                     /locus_tag="VFT30_11385"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU5093280.1"
                     /translation="MNVREVLEFAKKNKVQVVDMKFVDLIGTWQHFTIPVSELTEGLF
                     KDGSGLDGSSIRGWKAINNSDMLLVPDPVTACLDPFCSIPTLSLIGNVLDPITRETYD
                     RDPRYIAQKAEKYLQSTKIGDTSYWGPEAEFFIFDHARYDQTNHSGYYFIDSDEGVWN
                     MGQDGINLGGKIRHKEGYFPVPPTDTQQDIRTEMILEMEKAGIATEKHHHEVATAGQA
                     EIDIRFDSLVRTADKMMMFKYIVKNVARRHGKTATFMPKPIFGDNGSGMHTHQSIWKE
                     GKPLFAGKEYAGVSQMCLHYIGGILKHAPALAAFTNPSTNSYKRLTPGFEAPVLLAYS
                     SRNRSA"
BASE COUNT          229 a          315 c          306 g          289 t
ORIGIN      
        1 ggccgatcgg ttccgacttg aataggccag cagcacaggc gcttcaaaac ccggcgtcaa
       61 gcgcttgtag gagttggtag acgggttagt aaaggcggcc agcgccggcg catgcttgag
      121 aatgccgccg atgtagtgca ggcacatctg ggacacgccc gcgtattcct tccctgcgaa
      181 gaggggtttc ccctctttcc agatgctctg gtgcgtatgc atgccggagc cgttgtcgcc
      241 gaagatcggc ttgggcatga acgtggccgt ctttccatga cgccgggcga cgtttttgac
      301 gatgtatttg aacatcatca tcttatcggc ggttctcacc agactatcga agcgaatgtc
      361 gatttcggcc tggcctgcgg tagcaacttc atggtgatgc ttttcggtgg caattcccgc
      421 cttctccatc tcaagaatca tttccgtacg aatgtcttgc tgcgtgtcgg taggaggcac
      481 gggaaagtat ccttccttat gacggatttt tccgcctagg ttgattccgt cctgccccat
      541 gttccagacg ccttcgtcgg aatcgataaa gtaatagccg ctgtggttcg tttggtcgta
      601 ccgtgcatgg tcgaaaatga aaaattcggc ttccggtccc caataagagg tatcgccgat
      661 cttcgtgctc tggagatatt tctcggcttt ctgagcgatg tatcgtggat cgcgatcgta
      721 ggtctcccgg gtgatgggat caagcacatt gccgatgagg ctcaacgtcg gaatggagca
      781 aaacggatcg aggcaggcgg tgacggggtc cggcacgagc agcatgtcgc tgttattgat
      841 ggctttccac cctcgaatcg acgagccgtc aagcccagac ccgtccttga acagaccttc
      901 cgtcaactca ctgaccggaa tggtgaaatg ctgccaagtg ccgatcaggt ccacgaactt
      961 catatccacg acttggacct tgttcttttt tgcaaactct aagacctcac gaacgttcat
     1021 cccctcctcc tttcagacat tctgagagcc cacagcctcc ggcgttacgc cgctttgact
     1081 tcgagaaatt tttcgattgc ctgctcgatg actgttcgca cccgttccag ctgcacatg
//
LOCUS       DASTWE010001094         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_837323, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001094 DASTWE010000000
VERSION     DASTWE010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837323"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFT30_11390"
     CDS             complement(<1..692)
                     /locus_tag="VFT30_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HEU5093281.1"
                     /translation="MLRSLTKPSLAEVLVDQGILTQRTVEDVLRRLKGVAAVLGQTLI
                     SEGLLSEDQLAHALAVQCGLPYDPLTDFQVDPRYYETISIKLMQRFPFVPIAERSGAL
                     MIAVADPQNLLGLDELELLIGKPLDRVVSSKSAILAALERSEGSSQALRELEAEYRSI
                     LVKEDDRGEEIPTLDHAGEEQSPAVKLLDSILLSAMQRRASDIHIEAADRATKVKLRV
                     DGILIPAMEPLDV"
     gene            complement(751..1965)
                     /locus_tag="VFT30_11395"
     CDS             complement(751..1965)
                     /locus_tag="VFT30_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5093282.1"
                     /translation="MAVFAYRVARPDGSTIHGHVEGENESLVRAKLESQGWLVFNLHR
                     RGITSVKTEASWSWGKLPLSQFLVFNQELLALVKSGLPILRIWDLLIERAGHAGFQRT
                     LRDVREDIRGGTSASDALAKHPVYFPELYVATVKAGEQSGNLPEVLQRYVVYLKLMVG
                     LRQKVKKAISYPIVLIGIGLAVVGFLLTYVMPTFVSVYGESAKTLPWATQVLLDMVTH
                     AESRIVPAAVVMIGIMLGTHAYYATPTGQLVIDHLVLKLPLVGQIAVKHNTVQLTRTL
                     GTILAGGTPLVDALQGARGAVSNRWISQEMIGAVNEIREGATLADALDRSRVLPRLAI
                     EMLSVGEETGSLDTMLRDVAEFYEADLDTRLTQLTTWIEPVLLLVMGVLVGGIVIVMY
                     LPIFQMAGTVGG"
     gene            complement(2010..>2643)
                     /locus_tag="VFT30_11400"
     CDS             complement(2010..>2643)
                     /locus_tag="VFT30_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5093283.1"
                     /translation="VAELRAEVDARRQELADVQIARAQLEGRIREAERRLIEARHVID
                     LQREELASSRSEREQVRRTRAALQNQLKQIQKQISKVGKHVKVNVSPEVMASPKDGQR
                     EFRTFGSQEDALLASLKEGTQVVAGSAPQVSGASSGDEAPVVSQSATAPSRLQVLVKS
                     GDTLWSIARRYHTSVKFLMVRNALPSDRIQVGQVLWLTEPLIEEPEHERM"
BASE COUNT          611 a          777 c          630 g          625 t
ORIGIN      
        1 acatccagcg gctccatggc aggaattagg atgccgtcaa cacgaagttt gactttggtc
       61 gcgcgatctg cagcttcgat gtggatatca cttgcgcggc gttgcatcgc actcagcagg
      121 atagagtcca gcagcttcac ggccggactt tgctcttctc ctgcatgatc gagagttgga
      181 atttcttccc ctcgatcatc ttccttcacc aagatcgacc gatactccgc ttctagctca
      241 cggagcgcct gactcgatcc ttcactgcgc tccagcgccg ccagaattgc gctcttggaa
      301 ctgacgactc gatccagcgg ctttccgatt aggagctcca gttcatcgag cccaagcaag
      361 ttttgcgggt cggcaacggc aatcatcaat gctccggaac gctcagcaat cggcacgaaa
      421 ggaaatcgct gcattaactt gatcgagatg gtctcgtagt atcgtggatc gacctgaaaa
      481 tccgtcaacg gatcataggg aagaccgcat tgaacagcca gggcatgagc caattgatcc
      541 tccgagagga gtccttcact gatcaacgtt tgtcctaaga cagccgctac tcccttcaat
      601 cgtcttagga catcctcgac cgtgcgctgt gtgagtattc cttggtcgac caagacctct
      661 gcaagagatg gcttcgtaag gctacgtaac atacgcaacc tctttcatca ttatatcctc
      721 gttatcctcg gatcccactt ctcttctaat ctagccgcca actgtcccgg ccatttgaaa
      781 tatcggtagg tacatcacaa tgacgattcc accgaccaac actcccatga cgagcagcag
      841 aaccggttct atccacgtgg tgagctgcgt aagtctggta tcgagatcag cctcataaaa
      901 ctccgcgacg tcccgcagca tcgtatcaag cgaacccgtt tcctccccta ccgataacat
      961 ttcaatcgca agtctcggga gcactctgga acgatccaac gcatctgcca gggtcgcccc
     1021 ttcacgaatc tcattgactg ctccgatcat ttcttgtgaa atccatcggt ttgaaacggc
     1081 tcctcgtgcg ccttgcaggg catcaacaag cggggttcct ccggcaagaa tagttccgag
     1141 tgttcgagtc agctggacag tattgtgttt gactgcaatc tggcccacga gtgggagttt
     1201 cagcacgaga tgatcgatca ctagctgtcc cgtcggagtg gcatagtagg catgtgtccc
     1261 cagcatgatg ccgatcatga cgactgctgc gggcactatt cgtgactcag catgcgtgac
     1321 catatcgagc aagacctgag tcgcccatgg caaagtcttt gctgactctc catagaccga
     1381 tacaaaagtc ggcataacat aggtcaatag gaatcccacc acggccagac cgataccgat
     1441 gagaacaatg ggataggaga tcgccttctt taccttctga cgaagcccga ccatcaactt
     1501 cagatacaca acgtaccgct gtaggacctc tggaagattg cctgattgct ctcctgcctt
     1561 taccgtggcg acatagagtt ccggaaaata taccgggtgt ttagctaatg cgtccgaagc
     1621 cgaggttccg cctcggatgt cttcccgcac gtcacgtagt gttcgctgaa atccagcatg
     1681 gccggctcgt tcgatcaaga gatcccagat gcgtaaaatg ggcaacccgg atttgaccag
     1741 cgccagcaat tcttgattga agaccaagaa ctgactaagc ggaagtttcc cccatgacca
     1801 cgacgcctcc gtcttcaccg aggtgattcc tcggcggtgg agattaaaaa ccagccatcc
     1861 ttgtgactct agtttggcac gcaccaacga ttcattttct ccttccacat gcccatggat
     1921 tgtcgaccca tcaggccgtg cgactcgata cgcaaatact gccatagatg tagaccctca
     1981 cgacttgccg cttagccaat cgtgtgccat tacattcgtt cgtgctcggg ctcttcaata
     2041 aggggctcag taagccaaag gacttgccca acttgaatac ggtcgctagg aagcgcgttg
     2101 cgaaccatga ggaatttgac ggaagtgtga tatctgcgtg cgatactcca caacgtgtcc
     2161 ccagacttga ccaatacctg taagcgggaa ggtgcagttg ccgactgaga aaccaccggt
     2221 gcttcatcgc ccgaagatgc tcccgatacc tggggtgccg atccagcgac aacctgggta
     2281 ccctccttca aagaagccaa tagggcatcc tcctgagagc cgaacgtcct aaactcacgt
     2341 tgtccgtctt ttggagaagc cataacttca ggagacacat ttaccttcac gtgttttcct
     2401 actttcgata tctgtttttg aatttgcttc agttggttct gcagtgcggc tcgggtccgc
     2461 ctcacctgct cccgttcgga tcgcgagctg gccaattctt cccgctggag atcaatcaca
     2521 tggcgagctt caatcagtcg acgttccgct tcccgaatac gcccttcaag ctgagcccgg
     2581 gcgatctgca catcggccaa ttcctgacgc cgtgcgtcga cctctgccct cagctcggca
     2641 acc
//
LOCUS       DASTWE010001095         5058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1419377, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001095 DASTWE010000000
VERSION     DASTWE010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419377"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..565)
                     /locus_tag="VFT30_11405"
     CDS             complement(272..565)
                     /locus_tag="VFT30_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093284.1"
                     /translation="MKRDVVVSALPRVDSRRVTKLAEKSSDPLRNMAWRLEKLEGQIQ
                     KLSELVRDHAEDMDRLVRIVAENQDVVRRQLLRKHHEKVRVRKHLREANSGLD"
     gene            705..1943
                     /gene="glgC"
                     /locus_tag="VFT30_11410"
     CDS             705..1943
                     /gene="glgC"
                     /locus_tag="VFT30_11410"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU5093285.1"
                     /translation="MKNIFTMVLAGGKGERLFPLTDQRAKPAVPFGGKYRIIDFTLSN
                     CLNSGLRKIVVLIQYKSHSLDRHIRTGWNILNAELGEYIASVPPQQRISEDWYRGTAD
                     AVYQNMFLIDGEHPEFLLILAGDHVYKMNYAEMYHWLIAKSADAVVGAIDIPVKEATR
                     FGVIAIDEDYRITRFDEKPANPIPLPNDPAHAFASMGIYLFRTKAIREHLIADAREGS
                     AHDFGKNIIPRMIEQGRVYAFKFQDANKKAVKYWRDIGTLDAYWEANMDLVAVDPQFN
                     LYDSEWAIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIVSGGRVQNSILSPN
                     VRVQDHADVRESIILENVTIGAQSRIRRAIIDKDVTIPPQTEIGYNLEADAQRFTVTE
                     SGLVVISKGMKLHAAVDPSG"
     gene            1918..3777
                     /locus_tag="VFT30_11415"
     CDS             1918..3777
                     /locus_tag="VFT30_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU5093286.1"
                     /translation="MPPSIHPVDLVTALRHKHLTPAIVFLTSRRACDEAMETFDHADL
                     VLPPARQEAIAVALERITVQHPSIAEHPLIPIVKRMGVAAHHAGHLPSWKIAIEELMR
                     QGHLDAVFATTTLAAGVDFPARTVVITQSSIRKSRDFTDLTIGEIQQIAGRAGRRGKD
                     HVGFAIVTPSPYIDLTVLTKGLTGQPEAIDSQFTISYPMVLNLLKAHPHEQIQGILAK
                     SFAQFQLNQRAELLEQKLDALHIQMEPFGPRVCTDWITQWQTFDHARRHRHMRHQTLR
                     SESPEIVARLPFLTPGRVVGLSRGRGIVLRQYRSKGQKNSMLTVLRPDSAVTECPVTS
                     VKEVYDRTYDCAETPTYPWCSTETFDRLSHQLEELPPRLPVLPILVSAGIEPLPDAIV
                     QSLGDFPCPTCPSRPACQKDFIIASRLRQEQQRHTKSIQALRTSLWHRFQERVDVLQK
                     FGYLTLATQLTIEGEWARLIRIDHSLLITELIRAEAFTGADPSLLAGILASLAHDDDR
                     PGAFPRISPGLSSLLGQVRKLAESLSPYEDPPLLRADVAALVERWVADPTLTWIGLCR
                     LTTMAEGDIYRLLARTLEFLSQVQALKSTHPGLAESASQAITLIRRGVLEELS"
     gene            3887..4942
                     /locus_tag="VFT30_11420"
     CDS             3887..4942
                     /locus_tag="VFT30_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5093287.1"
                     /translation="MHTDELEQLLAQQPMALLHRLARGRVHRHFRAGKRRLIELLLQH
                     SIHNRAGFESDLQTLIGERHARSEPGSSHIHHLPAIAHKKSSPRGAEPDSTETAVSLS
                     AWLEGLGVPSPQPFVPDPWQNEALSALNETDVVISVPTGSGKTYVAVEAARRAMEDNR
                     SVIYTSPLKALSNTKYTEFSRIFGPEKVGILTGDRQENGQAPLLIMTTEILRNLLYDA
                     ASGEIDVRLDTLGLVILDESQYLADPERGVVWEETIIFCPSQARLLLLSASIGNPQDI
                     ADWLTSIRSTICRLVRHSKRTVPLRAGYLHPNGKLTPLFRTLGIPYGHPNQLHPEAKR
                     LFLEYEEETLPSGRPRR"
BASE COUNT         1154 a         1478 c         1312 g         1114 t
ORIGIN      
        1 aaccgagaca tccggaacat cctgactctt cggcggcgct tcatagggga tttgaatcgg
       61 ccatgtccgc acagactgat cggacaatgc atagagcgtc tgcgctttca ctagaccgac
      121 gagaataatc gcggcaatac ttgcatagct gatcatgacg tgtgaactcc tcttccagat
      181 catcgatgcg acacatttta gaggagagat tgacacgaac ctgtatccgg catcggctgg
      241 agcgaggggc gtaaccgaca gtcgtcgggc cttagtccaa ccctgaattg gcttctcgca
      301 gatgcttcct cacccgaact ttctcatgat gcttccgaag caactgccgt cggaccacat
      361 cctggttctc tgcgacaata cgaaccaatc gatccatatc ttcggcgtgg tctcgaacca
      421 gttcggacag cttctgtatc tgcccttcta acttttccag acgccaggcc atgttgcgca
      481 atggatctga tgatttctcc gcaagcttcg tcacacgcct ggaatcgact cgtgggagag
      541 ccgagacaac gacatctcgt ttcatgctca ctcctttgtt catgggtcga ccgatccgag
      601 acgctcgatt cggtgttctg gtggctagta ttatccgcat ggaaccggaa gtcaacgatt
      661 cagcttcctt tctatttcat gagggtcctg ctagaattca gccgatgaag aacattttca
      721 caatggtatt ggccggcggg aaaggcgagc gactttttcc gctgacggat caacgtgcga
      781 agccggctgt tcccttcggc gggaaatatc gtatcatcga ctttacctta agcaattgcc
      841 tgaactctgg actccgtaag atcgtcgttc tcattcaata caagtcgcac tcgctcgatc
      901 gccacatccg gaccggctgg aatattctca acgctgaact cggcgagtat atcgcttcgg
      961 tccctcctca acagcgcatc agcgaagatt ggtaccgtgg caccgccgac gccgtgtatc
     1021 agaacatgtt cttgatcgac ggagaacacc cggaattcct gctgatcctg gccggtgatc
     1081 acgtttacaa gatgaactat gcggagatgt accactggct catcgccaag agcgcggatg
     1141 cggtcgtcgg tgccatcgac attcccgtca aggaggccac tcgcttcgga gtcatcgcaa
     1201 tagacgaaga ctaccgaatt actcgcttcg atgaaaagcc ggcgaatccc attccactac
     1261 ccaacgatcc cgcccacgcc tttgcgtcga tgggcatcta cctgttccgc acaaaagcga
     1321 tccgcgagca cctgatcgcc gacgcacgag agggcagcgc acatgacttc gggaaaaata
     1381 tcattcctag aatgatcgag caagggcgtg tctatgcctt caaatttcaa gatgccaaca
     1441 aaaaagccgt taagtactgg cgagacattg ggacgctcga tgcttactgg gaagccaaca
     1501 tggatttggt cgccgtcgat cctcaattta acctgtacga ctcggaatgg gcgatccgaa
     1561 cctatcaagg acagtttcca ccagccaagt tcgtcttcgc acaggatttt cagggaggac
     1621 ggatgggagt cgccctcgat tcgatcgtct gcggcggctg catcgtttcg ggtggccgag
     1681 tccagaattc gattttgtcc ccgaacgtcc gcgtgcaaga ccacgcggat gtccgtgaat
     1741 ccattatctt ggaaaacgtg accatcggcg cccagagccg catcagacgc gccatcatcg
     1801 acaaggatgt gacgatccct cctcagacgg aaatcgggta caatctcgag gccgatgctc
     1861 aacgatttac cgtcactgaa tcaggcctcg tggtcatctc aaagggaatg aagctgcatg
     1921 ccgccgtcga tccatccggt tgatcttgtc actgcgctcc gccacaagca tctcacgcca
     1981 gccatcgtgt ttctcacctc acgtcgggcc tgtgacgagg ccatggagac gtttgaccat
     2041 gccgatctcg tgctccctcc ggcacgacag gaggcgatcg ccgtcgcgct tgaacggata
     2101 acggttcagc acccaagcat tgctgagcac ccgttgattc ccattgtgaa gaggatgggg
     2161 gttgccgccc atcacgccgg gcatttgcct tcatggaaaa tcgcgatcga agagctgatg
     2221 cggcaaggcc acctcgatgc ggtattcgcc acgacgacct tggcggcggg tgtcgatttt
     2281 ccagctcgta cggttgtgat cacccaatcg agcatccgca agtcccgcga cttcaccgac
     2341 ctcaccattg gcgaaatcca gcagatcgcc ggacgagctg gccgccgcgg aaaagatcac
     2401 gttgggtttg cgatcgtgac tccttctcct tatatcgatc tgacggttct gaccaaagga
     2461 ttgaccggac agccggaagc catcgacagc cagtttacaa tcagctaccc gatggtcttg
     2521 aaccttttga aagcccatcc ccatgagcag attcaaggaa tcctggccaa gagtttcgct
     2581 caattccagc tgaatcagcg cgctgagctt ctggagcaaa aactagacgc actccatatc
     2641 cagatggaac cgttcggccc tcgcgtctgt acggattgga tcacccaatg gcaaaccttc
     2701 gatcatgcgc gaagacaccg ccacatgcgc catcaaactc tccgctcaga atcgccggag
     2761 attgtagctc ggttgccgtt cctgacacct gggcgcgtcg taggactcag caggggccgt
     2821 gggattgtgc tccggcaata tcggagtaag ggccaaaaga attccatgct caccgtctta
     2881 cgaccggaca gcgccgtcac cgaatgccct gtcaccagcg tgaaagaagt ctacgaccgc
     2941 acgtacgact gcgcggagac gccgacatat ccttggtgtt ccaccgaaac gttcgatcga
     3001 ctcagtcatc agttagaaga actgccgccg cgtttgccag tgctgcctat actcgtatcc
     3061 gcgggtatcg agccactacc tgatgccatc gtccaatcgt tgggtgactt cccttgcccg
     3121 acttgccctt cacgaccggc ctgccaaaaa gacttcataa tcgcatcacg gcttcgccag
     3181 gaacaacagc gccatacgaa atcaattcag gcactgcgga ccagtttgtg gcatcgtttc
     3241 caagagcgcg tggacgtgct gcagaaattc ggatatctca ccctggcgac acaattgacg
     3301 atcgaaggag aatgggcaag gcttatccgg atcgatcatt cgctgctgat taccgaactg
     3361 attcgtgccg aagcctttac gggagcagat ccgtccctcc tcgccggcat cttggccagt
     3421 ctggcccacg acgatgatcg tcccggggca tttccgcgca tcagtccggg gctgagttcc
     3481 ctgctcgggc aggttcgcaa gttggcagag agtttatctc cttacgagga tcctcccctc
     3541 ttacgcgccg atgtagcggc cttggttgag cgctgggtcg ccgatcccac tctcacatgg
     3601 atcggactct gtcggttgac cacgatggca gaaggcgata tctaccgatt attggctagg
     3661 accttggagt ttctatctca ggtgcaggca ttaaaatcga cgcatcccgg cctcgcagaa
     3721 tccgcttcgc aagcgatcac gttgatccga cggggagtgt tggaggaact gtcgtgagga
     3781 gaggcaaata ctcgtcagtc aatagccatc agtcagtgga gaggctgaaa aaaatcgtct
     3841 ggtccgttaa tggccagtga ccaatgacgg atgaccgggg ggttttatgc ataccgacga
     3901 gcttgaacaa ttacttgcac agcaacccat ggcgctcttg catcgcctgg cccgtggacg
     3961 cgtccatcgg cactttcgtg ccggcaagag gcgactgatc gagctgctcc ttcaacactc
     4021 gatccacaac cgtgccggat tcgaatcgga tctgcagacc ttgatcggag agcggcacgc
     4081 tcgatcagaa cctgggtcgt cccacatcca tcatcttccg gcgatcgctc ataaaaaatc
     4141 atcgccgcgg ggagcagagc cggattctac tgaaaccgcc gtctcactat cggcctggct
     4201 ggaaggcctc ggcgttcctt ctccgcaacc atttgtgccg gacccctggc agaacgaggc
     4261 tttgtccgca ctgaatgaga ccgacgtcgt gatcagcgtc cccaccggaa gcggaaaaac
     4321 ttatgtggcc gttgaagccg cccgtcgggc catggaagac aatcgcagtg ttatttacac
     4381 atcgccgctc aaggccttgt ccaataccaa atatacggag ttttctcgaa tcttcggtcc
     4441 tgagaaagtc ggcattctca ccggagatcg ccaggaaaac gggcaagcgc cattgctcat
     4501 catgaccaca gaaattctac gcaacctgct ctatgatgcc gcaagcgggg agatcgatgt
     4561 cagattggac acgttgggac tcgtgatttt ggacgaatcg caatacctcg ctgatcccga
     4621 gcgtggagtc gtatgggaag aaactatcat tttttgtcct tcccaggctc gattactgtt
     4681 gctatccgcc tcgatcggca atccgcaaga catcgccgat tggctgactt caatccggtc
     4741 aactatttgt cggctcgtac gtcacagtaa acgcaccgta cccctcagag ccggttatct
     4801 gcatccgaac gggaaattga cgcctctgtt tagaacgttg ggaatcccct atgggcaccc
     4861 caaccaactt catccggagg ccaagcgcct ctttcttgag tatgaagaag aaaccctacc
     4921 ctccggaaga ccgagacgat agctcgtcca ctagttgagc agctaaaagg acccggtcag
     4981 cccgacgatg acgccatggc ggattgtctg caactcattg agaggagcgg tccgggactc
     5041 gcttccagca ggatgact
//
LOCUS       DASTWE010001096         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1386738, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001096 DASTWE010000000
VERSION     DASTWE010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386738"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..338)
                     /locus_tag="VFT30_11425"
     CDS             complement(66..338)
                     /locus_tag="VFT30_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093288.1"
                     /translation="MARAISPSDASPSNDNPADQTWDVELLRVLVSRKGTQVEAQWAI
                     HPQLKQDLLPNEWQEVMDLMAKATDIVGDRFSKALSEADPMPPGNA"
     gene            complement(407..>1134)
                     /locus_tag="VFT30_11430"
     CDS             complement(407..>1134)
                     /locus_tag="VFT30_11430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5093289.1"
                     /translation="LKYAGAGFHIEYKNPINLVTDADRAAEQCVIDRIKAHFPTHRFL
                     AEERGRLEEEQSPYLWIIDPLDGTTNFAHGYPAYCVSIGLEYEGRCVLGAIFDPSRNE
                     LFTAIEHRGAQLNGRTIHVSNAMTLDSSLLVTGFAYDIRETPRNNLDHFAKFALKAQG
                     LRRTGSAALDLCYVAAGRFDGFWEVRLNPWDMAAGSVIVREAGGRLSDFSGKDLSIYG
                     QELVASNGQIHEAMLTVLNQEIP"
BASE COUNT          259 a          310 c          297 g          268 t
ORIGIN      
        1 tacgcatcca tacgaacctc ctcgattgat tgagcgtgga atagagacgg ggagcggtga
       61 gacaatcagg cattgcccgg aggcatgggg tcggcttcag acagggcttt ggaaaagcga
      121 tcgccgacga tgtcggtggc cttcgccatg aggtccatta cttcttgcca ttcattgggc
      181 agcaggtcct gcttcaactg aggatggata gcccattggg cctcgacttg tgttcctttg
      241 cggctgacca gcacgcgcag cagttccaca tcccaggtct ggtcagcagg attatcgttg
      301 gacggcgatg cgtcagaagg agatatagcc cgcgccatac ataactctta gtgtaaaccg
      361 ttcacacagc atactgtatt ctgtaccatt ctgtatatcc tgtatatcaa ggaatctctt
      421 ggttgaggac tgtaagcatc gcctcgtgga tctgtccatt gcttgccacg agctcctgtc
      481 cataaatcga aagatctttt ccgctgaaat cactcagccg cccgccggcc tctcgtacga
      541 tcaccgaccc ggccgccata tcccatggat tgagccgaac ttcccagaac ccatcgaatc
      601 gtcctgctgc aacatagcag agatccaatg ctgcggatcc cgttctcctg agtccctggg
      661 ccttgagggc aaatttggcg aaatgatcaa ggttattgcg cggtgtttcc ctgatgtcat
      721 aggcaaagcc cgtcaccaac aggctgctgt cgagggtcat cgcattggac acatggatgg
      781 tgcgaccgtt caactgcgcg cctcgatgtt caatggcagt gaatagttca tttcgtgagg
      841 gatcgaagat tgcgccaagc acacaacgcc cctcgtactc gaggccgata gacacgcagt
      901 aggctggata gccatgcgca aaatttgttg tcccatccag cggatcgata atccacaaat
      961 agggcgactg ctcctcttct aggcgacccc gctcctcggc caagaaccga tgggtaggaa
     1021 aatgagcttt gatccggtcg atgacgcact gctcggcagc gcgatctgcg tcagtgacta
     1081 gattgattgg attcttatat tcgatgtgaa aaccggcccc tgcatattta agca
//
LOCUS       DASTWE010001097         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_999314, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001097 DASTWE010000000
VERSION     DASTWE010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999314"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1085
                     /locus_tag="VFT30_11435"
     CDS             189..1085
                     /locus_tag="VFT30_11435"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5093290.1"
                     /translation="MANLGFLGLGIMGYPMAQHLLKAGHRVALWTHTAAKAKELAKEG
                     NGIACATPKEVAQQADCIFYCVGDTAMSEQVALGSDGIIEGAKPGTVVVDASTVSPSG
                     SRKIGKALAEKKIDFLDAPCTGSKPGAEGGRLTFMVGGDKAVFEKVKPYFEPMGKNLY
                     YCGGPGLGLQAKLTQNLVLSNILQAFNEGFVLATKGGVDPKLMLDILENSAAKSGLIA
                     FKAPFVFKRDFSTNFSVKWMHKDIGLMLDSGNELDVPLPLTSLTQQMFRSAIAKGYGD
                     DDICSTIKVLEEIVGVEVKADM"
BASE COUNT          269 a          272 c          339 g          250 t
ORIGIN      
        1 tttcgcactt ttccgaactg gaaacacgtc gccatcacga ttttgctggg attcttctgc
       61 ggccgggcct tccagaaggc gcaatctgtc cgggccgcca tggtcactca tgccctagtg
      121 aatgcgacat gggtggggtt cctggggaaa gcctgaaaag ccaaagaccg cgatacaatc
      181 ggcgttctat ggcgaatctt ggatttctcg gacttggaat catgggttat cccatggcgc
      241 aacatctgct gaaggcaggc catcgggtgg ccctatggac gcacactgcc gccaaggcaa
      301 aggaactggc gaaggaaggc aacggaatcg cctgcgcgac tccgaaggaa gttgctcaac
      361 aagcggattg catcttctat tgcgtcggcg acacggctat gtcggagcag gtggctctgg
      421 ggtcggacgg gatcatcgag ggagccaagc cggggacggt cgtggtcgac gccagcacag
      481 tctcgcctag cggtagccga aagatcggaa aagcgttggc agaaaagaag atcgactttc
      541 tggacgctcc ttgtaccggc tccaagccag gagcggaagg cggtcgtctg acgttcatgg
      601 tcggcggtga caaggcggta ttcgaaaaag tgaagccgta ctttgagccg atgggcaaga
      661 atctttacta ctgtggcggg cccggcctgg gactgcaggc gaagctgacg cagaatctgg
      721 ttctttccaa tattttgcaa gcgttcaacg aaggcttcgt gctggcgacc aaaggcggcg
      781 tggatccgaa actgatgctg gatatcctgg aaaactctgc cgcgaagagc ggtttgatcg
      841 ccttcaaggc tccctttgtc ttcaagcgtg atttttccac aaatttctcc gtgaaatgga
      901 tgcataagga catcgggctg atgttggact caggaaatga gctggatgta ccgctgccgc
      961 tgaccagtct gacgcagcaa atgttccgga gcgccatcgc aaaaggttat ggcgatgatg
     1021 atatctgctc tacaatcaag gttttggaag agattgtcgg ggtagaagtg aaagcggata
     1081 tgtaaaattt cctgtattca gtagaaaaag ttcttgcaat tccaaaactc
//
LOCUS       DASTWE010001098         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1031482, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001098 DASTWE010000000
VERSION     DASTWE010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031482"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFT30_11440"
     CDS             complement(<1..325)
                     /locus_tag="VFT30_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEU5093291.1"
                     /translation="MSVIKGIKGVKDLFPEETPRWRLIEETARRWAARYGFHEIRIPI
                     FEVTTLFARSIGASTDIVEKEMYTFQDRDGTSLTLRPEGTAGTVRAYIEHNRAAIPVP
                     QKYFYF"
     gene            480..1967
                     /locus_tag="VFT30_11445"
     CDS             480..1967
                     /locus_tag="VFT30_11445"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5093292.1"
                     /translation="MSQDQSGRRVVAIAPMPPEKSAYALARYSRSPDSIEQSIRWVHG
                     HSSEKFWEQFYFDYGHASIADLGHVIVCLEEISEFAAIRLEDEPLWDGQAKSSRYQNF
                     ASSRWFVPGQIRGSETEAIYEGILRSLSEIYRLLHDPLVTYLSERDPCPDSMKQADYQ
                     RTIAARAFDVTRYLLPLAAKTNVGQVVSIRTLEKQITRLLSSQLPELRAIGEDLKEAC
                     QRPAVNLWNELSGQTAGLNDPLAPTLARHAKASAYQESVYSDLARYAKEALRGTGLEQ
                     PGTWGAVESVELIDPHHPLDEIVTTLLYRVSQAPYRKILEVVKEWSEKEKQATIEVAI
                     RQRGPYDELIKEFRSGYTFNLDILMDIGAWRDMHRHRRCQQVQQNFTTLHGYDVPPPL
                     IEAGLDQEYRQAMDAVRQDIELLKKKDQEASLYAIPFGFKVRCLFKMDYAEAEYISKL
                     RSGVKGHWSYRTVAWKMKQQLTRRYPYLGEQIHATPPDVEDALTR"
     gene            1983..>2337
                     /locus_tag="VFT30_11450"
     CDS             1983..>2337
                     /locus_tag="VFT30_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093293.1"
                     /translation="MSIHQQQCEAALVAGAWDALFAESLAWSRDPDGAKDPRPLFARN
                     VVYLLQGRFADAWKTHALCLEAEKDIAAVGTWVKDLLDRHTDSGYTHLVMGLFLAQSG
                     RSEQSMRPYAEAARLL"
BASE COUNT          539 a          638 c          662 g          498 t
ORIGIN      
        1 cgaaatagaa atatttctga ggaacaggaa tcgcggcacg attgtgctcg atataggccc
       61 ggacagttcc ggcagttcct tcaggtctga gggtcagcga ggtaccgtcc cggtcctgga
      121 acgtatacat ttccttctca acaatatcgg tcgatgcacc gatgctccgt gcaaacaagg
      181 tcgtcacttc aaagatcgga atgcgaattt catgaaatcc ataccgcgcc gcccaccgcc
      241 tggcagtctc ttcgatgagg cgccaacggg gcgtctcttc cggaaacaga tctttgacac
      301 cttttatgcc cttgatcacg gacacccgtc atctccttag gaaagtgcgt ttacactgca
      361 cagttcgcgc ggactatagc ggcggcttct gcggcaagtc aacggatccg caacttgcac
      421 cataccgacg tgagaggtat agtgcgcgac ctatagtcaa tacgactgaa agactttcta
      481 tgagtcaaga tcaatcaggt cgccgggtcg tggccatcgc tcccatgcct ccggaaaaat
      541 ctgcttatgc gcttgcgcgc tatagccgat cgccggactc catcgaacaa agcatcaggt
      601 gggttcatgg acactcctcg gagaagttct gggagcagtt ttatttcgat tacggtcatg
      661 cgtcgatcgc cgacctcggc cacgtcatcg tctgtcttga agagatctca gaattcgccg
      721 ccattcgtct ggaggacgag ccgctctggg atgggcaggc gaaatcgagc cgctatcaga
      781 attttgcctc gagcaggtgg tttgtgccgg gccagattcg cgggagtgaa acggaggcaa
      841 tctatgaagg cattctccgc agtctgtcag aaatttatcg gttactgcac gatcccttgg
      901 tcacgtacct ctctgaacgg gacccctgtc cggactccat gaaacaggct gattatcaga
      961 ggacgatcgc ggcacgcgcc tttgatgtca cccgctacct gcttccgctt gcggccaaga
     1021 ccaacgttgg gcaagtcgtc agcatccgga cgttggaaaa gcagattacg cgcttgttgt
     1081 cgtcgcaatt gccggaattg cgggcgatcg gcgaagattt gaaggaagcc tgccaacggc
     1141 cggcggtgaa tttgtggaac gaactctccg gacagaccgc aggtttgaac gatccactgg
     1201 caccaacgct ggcacggcat gccaaggcga gtgcttatca agaatcggtt tactccgatc
     1261 tggctcgcta tgcgaaggaa gcattacgcg gcacagggtt ggagcagcct ggtacgtggg
     1321 gtgctgtaga gtcggtcgag ctgatcgatc cacatcatcc gctcgacgaa atcgtcacga
     1381 cgctcctcta tcgcgtgtcg caggccccgt atcggaagat cctcgaggtc gtgaaagagt
     1441 ggtccgagaa ggagaaacaa gcgaccatcg aagtggccat aaggcagcgg gggccatacg
     1501 atgaactcat caaagagttc cgcagcggct atacattcaa cctcgatatc ctcatggaca
     1561 tcggcgcctg gcgagatatg caccgccatc ggcggtgcca gcaagtgcag caaaatttca
     1621 cgactctcca tggctatgat gtcccgccgc ctcttattga ggcagggctt gatcaggagt
     1681 atcggcaggc gatggatgcc gtgcgtcagg atatcgagtt gctcaagaaa aaggatcagg
     1741 aagcatcgct ctacgcaatt ccgttcggct tcaaagtgcg ctgtttgttc aaaatggatt
     1801 atgctgaagc ggaatacatc agtaagctac ggtccggcgt caaaggccac tggtcatatc
     1861 gaacggtcgc gtggaagatg aagcaacaac tcacccgacg atacccctac ctcggtgaac
     1921 agatccacgc gacgccaccc gatgtcgaag acgcgctgac tagataagac gttgcctcac
     1981 aaatgagcat ccatcagcaa caatgcgaag ccgcgctcgt ggcgggagca tgggacgcct
     2041 tgttcgccga gtcgttggct tggagccggg atccggacgg ggcgaaggat ccacgtcctc
     2101 tctttgcacg aaacgtcgtg tatctcctgc agggtcggtt tgccgatgcc tggaaaaccc
     2161 atgcgctctg cctggaagct gagaaggaca tcgcggccgt gggaacgtgg gtcaaggatc
     2221 tgctcgatcg gcatacggac agtggctaca cccatttggt gatgggcctt tttttggcgc
     2281 aatcgggtcg atcagagcaa tccatgagac catacgcgga agccgctcgg ttactcc
//
LOCUS       DASTWE010001099         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_740280, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001099 DASTWE010000000
VERSION     DASTWE010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740280"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1251
                     /locus_tag="VFT30_11455"
     CDS             <1..>1251
                     /locus_tag="VFT30_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5093294.1"
                     /translation="KQDGQGYLLSGRRSYIYLFDLEAKKLERLTTTKFDESSPAWSPD
                     GSRIAFMSNHGDEPDRDPAAQLYIADAKPGSTEKQLTPNATRAGRGRPEWSPDSKWIV
                     LLEGDEKKYGAYSMDHVILVATDGSAATPTPFKPTEDLDRGVSSPKFSADGRSLTFLV
                     TDDRSVYPARANVSGGVIEKLMQPPIVMSSWNTAGGRTVVMSGGDMKGNEVYEFANGN
                     LRQVTHQNDALIAELDLGTTERVNFKSKDGTDIGGLLTYPVGYVKGTKVPLLLRIHGG
                     PNGQDQHTLSIERQFFAANGYAVLAVNYRGSSGRGQKFSRSIFADWGNYEVQDLLAGV
                     DHVIKMGVADPDRLGVGGWSYGGILTDYLIASDTRFKAATSGAGTAFTVAFYGTDQYI
                     IQYDHEIGPPWNPKAWETYVKISYP"
BASE COUNT          315 a          332 c          337 g          267 t
ORIGIN      
        1 caaacaggat ggccagggct accttctgtc aggtcgacgt tcgtacatct acttgttcga
       61 ccttgaagcc aagaaacttg agcgactgac gaccaccaag tttgatgaga gttctccagc
      121 ctggtcaccg gacggaagtc gaatcgcttt catgagcaat cacggcgacg aacctgatcg
      181 tgacccagcc gcccaactct acatcgcaga cgcgaagcct ggttcaacag aaaaacaact
      241 gacgccaaac gcgactcgcg ctggacgtgg ccgtccagag tggagtcccg acagcaaatg
      301 gatcgtgcta ctcgaaggcg atgagaagaa gtacggggca tacagcatgg atcatgtgat
      361 ccttgttgcg accgacggta gcgcagccac cccaactccg tttaaaccta ctgaagatct
      421 cgaccggggt gtctcgtctc caaaattttc agccgatggt cgctcattga cattcctggt
      481 aaccgacgat cgttccgtgt atccagcacg cgcaaatgtc tcgggcggtg taatcgaaaa
      541 gcttatgcag ccaccgatcg tgatgtcttc ctggaacacc gctggcggcc gaactgtcgt
      601 gatgtcgggt ggcgacatga aaggcaacga ggtttatgag tttgcaaacg gtaaccttcg
      661 acaagtaaca catcagaacg acgccctgat tgctgaactt gatctgggca caaccgaacg
      721 agtcaatttc aagagcaaag atggcacaga cattggtggc ctgctcacct acccggtggg
      781 ctatgtgaaa ggcacaaagg tcccgcttct acttcgcatt cacggtggac caaatggaca
      841 agaccagcac acgttgagca tcgagcgaca gttcttcgcg gctaatggtt acgcggtgtt
      901 ggcggttaac tatcgcggca gctcgggtcg aggacagaag ttttcacgat cgatctttgc
      961 ggattggggt aactatgaag tccaggatct tttggctggc gtcgaccatg taatcaagat
     1021 gggcgtcgcg gacccggatc gacttggagt tggcggatgg agttacggtg gaattcttac
     1081 tgactacctg atcgcaagtg ataccagatt caaggctgcg acaagcggcg ccggaaccgc
     1141 cttcacggta gccttctacg gcaccgacca gtacatcatt caatacgatc acgaaattgg
     1201 accaccctgg aatccaaagg cttgggaaac ctacgttaag atttcctatc c
//
LOCUS       DASTWE010001100         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_126489, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001100 DASTWE010000000
VERSION     DASTWE010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126489"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VFT30_11460"
     CDS             <1..1295
                     /locus_tag="VFT30_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093295.1"
                     /translation="GLIVAAFSKTVRTEIRRFIARNFYRSKYDYRAQWLQITEAFQEA
                     ADKNSIMNRLMDVLIRTFPATHVSIWSFREADRRFFRVRSMAGEIESEPIELSHSIVT
                     ELGKRDSAVWVEPQATETGDHLFMLTDSIRSSGVALYFPIRAHERVIAFIALGPQPHG
                     ELYGTDDEDLLRGIAHHVGMLLSHAILTEDRRASAELEALHRFSAFCLHDLKNLAMRL
                     SLVAQNAERHGRDPAFQESAMRTVKDTALKMAALMSKLSRKSLRPLQAGTSELVDLSA
                     VLDDIVAPMRRDPAARLSVTVVPVPPIMAVRDQIHQVLLNVVLNAKQAIGGNGEISVA
                     LAQHNGSALVTVDDTGDGIPPEMLESLFRPSQSSRSGGLGIGLYQCRQIVEAHQGTIQ
                     VRSEIGKGTQVRIELPIHQTAGNREETGLPHTAMSVPV"
BASE COUNT          315 a          410 c          426 g          315 t
ORIGIN      
        1 ttggattgat cgttgccgcg ttctccaaga cggtgcgtac cgagattcgg cgtttcatcg
       61 cgaggaattt ttatcgatcg aaatacgact accgtgcgca gtggctccaa atcaccgagg
      121 cgtttcaaga ggcggcggac aaaaactcca ttatgaaccg tttgatggat gtgctgatcc
      181 gaaccttccc ggccacccat gtctcgatct ggtcatttcg cgaggccgat cggcgatttt
      241 tccgcgttcg ctcaatggct ggtgaaattg aatcggagcc gatcgaacta tcccattcga
      301 ttgtcacgga acttgggaag cgggatagtg cggtctgggt agaaccacaa gcgaccgaaa
      361 cgggagacca cctcttcatg cttacggatt ccatccggtc gtctggggtg gcgctctatt
      421 tcccgatccg cgcacacgag cgagttatcg ccttcatcgc gttaggccca caaccgcacg
      481 gcgagctgta tggcacggac gatgaagatc tgctgcgcgg aatcgcccat cacgtgggga
      541 tgctgctctc ccacgcaatc ttgacagagg accggcgggc gtcggctgaa ttggaggcac
      601 ttcaccggtt ctccgcgttc tgtttgcatg acctgaaaaa tttggcgatg aggctttcat
      661 tggtcgctca gaatgccgaa cgccatggcc gggatccagc gttccaggaa tcggcgatgc
      721 ggacggtcaa agacaccgcc ctcaaaatgg cggctctgat gagtaaactg tcccgaaaat
      781 ccttgagacc gctccaggcc ggaacgtctg agctggtgga tctgtctgct gtgctggatg
      841 acatcgtcgc gccgatgaga cgggacccgg cggcacggct gtccgtcaca gttgtgccgg
      901 ttccaccgat catggcggtg cgcgatcaga ttcaccaggt cttgctcaac gtggtgctga
      961 acgcgaaaca ggctatcgga gggaatggcg agatctccgt cgcattggca cagcacaacg
     1021 gttcggccct tgtcacggtt gatgatacgg gagacgggat tcctcccgag atgctggagt
     1081 cgctgtttcg cccatcgcaa tccagccgct ccggtggact tggaatcgga ctgtaccaat
     1141 gcaggcaaat cgtggaagcc caccagggga ctattcaggt tcgcagcgag atcgggaagg
     1201 gaacccaagt gcgaatcgag cttcccatcc atcaaacagc cggcaatcga gaggaaaccg
     1261 gcttgcctca cacagccatg tccgtgccgg tttaacacag gggaattcca atcatgacaa
     1321 tacggcctgt tttgcttctt gtcgacgatg atgcagctat tcacgaccag atgaaatggg
     1381 cccttgcatc ggactacgac ctattggagg catcggatcg tacgggtgcc ctatcgcaca
     1441 ttcgccaggc caagccaccg ctggtg
//
LOCUS       DASTWE010001101         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_61795, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001101 DASTWE010000000
VERSION     DASTWE010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61795"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1327
                     /locus_tag="VFT30_11465"
     CDS             41..1327
                     /locus_tag="VFT30_11465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5093296.1"
                     /translation="MDTSVHVSFHALGLSEALLKDLVEAGFASPTPIQERAIPPALAG
                     RDVIGCAQTGTGKTAAFVIPIIERLALLPKGQPQALILAPTRELALQTSATIDKLGKR
                     RRVSATVIVGGMDMQAQVRGLRQRPDILVATPGRLLDHMWSGTIMLSSIKTLVLDEAD
                     RMLDMGFAPQINQILDALPEQRQTLLFSATLPADLARLVQASVNNPVRVMVAPSASTA
                     EGVTQAVHYTSHADKPDLLLSLLGADQATALVFTRTKHRADRLGRFLGHAGHRVAVLH
                     GDRSLSQRRAALEGFRRGAFRVLVATDIAARGIDVANIGHVINFDLPNCPEDYVHRIG
                     RTARMKTTGRATSFVTGEDQRQLRDIERLLGQAVPLAPGSGSHSPAIPVPGGGVRRDG
                     HPSRSQEKRSQTHSFRNARKPNAEPSSYRVATAIPS"
     gene            1391..1768
                     /locus_tag="VFT30_11470"
     CDS             1391..1768
                     /locus_tag="VFT30_11470"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5093297.1"
                     /translation="MLGIQQPEVAENKARRYPRLRILIPFSCSLSSLGTRWFRKSVSD
                     VGMVYDLSLHGACVSTDAAIKPGDQISLTLRLTKGTPPTEVAVATVCWTNHQLHGLAF
                     RTLSQASLRQLTEYMNASDRGKE"
     gene            1775..2173
                     /locus_tag="VFT30_11475"
     CDS             1775..2173
                     /locus_tag="VFT30_11475"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5093298.1"
                     /translation="MDDLDVRAVSGQNVPQLRKHPRVRVSAPFPCSLARVGLLRNGAV
                     EQGLGIIYDVSAKGARVMTEAVITPGDRIAMRLRLPDQAASMFIETATVRWGKEQTYG
                     VEFERLSPNDNKHLQSFMNHQSRSGQSLAV"
     gene            complement(2215..>2698)
                     /locus_tag="VFT30_11480"
     CDS             complement(2215..>2698)
                     /locus_tag="VFT30_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5093299.1"
                     /translation="PSLIFAVIRTESNFNPFAVSSAPAYGLMQLVPTSGGRDAYRRVK
                     GKDTIPSRDYLFDPENNIELGSAYLNVLSYNLLEQIDNDVSREYCVISAYNTGPRNVF
                     KTFAGDSVAALNQINRLQPPAVYDRLRNNLPYQETRDYLVKVVTFRKQFVSLREDNDR
                     "
BASE COUNT          594 a          719 c          776 g          609 t
ORIGIN      
        1 ctgatcccgt catcatgcga tcaaactgaa aggatatgta gtggatacgt ctgttcatgt
       61 aagttttcac gctctcggtt tgtccgaagc acttttgaaa gatttggttg aggcaggatt
      121 tgcttcaccg actcccatcc aagaacgagc catcccgcct gcccttgcgg gacgagatgt
      181 catcggatgc gctcaaaccg ggacagggaa gaccgctgcc ttcgtcattc caatcatcga
      241 gcgtctcgct ctgcttccga aagggcagcc ccaggcattg atcttggcgc cgacgcgcga
      301 actcgcactg caaacctcgg ctacgattga taaactcgga aagagacggc gcgtttccgc
      361 caccgttatt gtgggaggca tggatatgca ggcccaagtc cgaggtttgc ggcagcgacc
      421 ggacatcttg gtcgcgacgc cgggccgcct ccttgatcac atgtggagcg gcacgatcat
      481 gttgtcgtcc attaagacgt tggtgttgga cgaagcagat cggatgttgg acatgggctt
      541 tgccccgcaa atcaaccaga tactcgatgc cttgccggag cagcggcaga cgctgctgtt
      601 ttcggctacc ttgcctgcgg atttggcgcg gctggtccag gcgagcgtga acaatccggt
      661 gcgagtgatg gtcgcgccgt ctgcctcgac ggccgagggt gtcacacagg cggtgcatta
      721 cacatcccac gccgacaaac ccgaccttct gttatcccta ttgggagctg atcaagctac
      781 cgcgttggtc ttcaccagga cgaagcatcg tgccgatcgg ttgggacgct ttctgggtca
      841 tgcgggccat cgagtggctg tactgcatgg agaccggagt ctgtcgcaaa ggcgcgctgc
      901 gctggaaggt ttcaggcgcg gagcattccg tgtgctggtg gcgacggata tcgctgccag
      961 agggatcgat gtcgcgaata tcggccatgt gatcaatttc gatcttccca attgcccgga
     1021 agattacgtg catcgtattg gtcgtaccgc tcggatgaag acaaccggtc gcgccacaag
     1081 ttttgtgacg ggcgaagacc agcgccagct acgagatatc gagcgtttgt tggggcaagc
     1141 agttccgctt gctccaggaa gcgggagtca ttcccctgcg attcctgtcc ctggcggagg
     1201 tgttcgtcgg gatgggcatc cttcgagaag tcaggaaaaa cgttcccaaa cccactcctt
     1261 ccgcaacgcg agaaagccca atgccgaacc atcatcgtat cgtgttgcaa cggcaatccc
     1321 ttcataaagt gcaaaaatag ttcgctgaat tcgccttgtg aggtagagtg tccctcacga
     1381 ggtggatttc atgctgggga ttcagcagcc ggaggtggca gaaaacaaag ctcggcggta
     1441 cccccgactg cgcattctca tacccttcag ctgttccctt tcctcgctgg gaaccaggtg
     1501 gtttcgaaaa tcggtcagtg atgttggtat ggtctatgac ctgtctctgc acggcgcatg
     1561 cgtgagcacc gatgcggcca tcaagccggg cgaccagatt tccctcacac tccgcctgac
     1621 caagggcact cctccaacgg aggtggcggt tgccaccgtt tgctggacga atcatcagct
     1681 ccatggactt gcgttcagaa cgctctcgca agcatcgttg agacagttga ctgagtacat
     1741 gaatgcttca gatagaggga aagagtaaga cacgatggat gatctagacg tcagagccgt
     1801 cagtggacag aatgttccac aattgcgcaa acaccctcgg gtgcgtgtgt cagctccatt
     1861 tccctgttcg ttggccaggg tcggtttgct gaggaacggg gcagttgaac aaggactggg
     1921 gatcatctat gacgtctcgg caaagggggc gcgtgtcatg acggaagcgg taatcacacc
     1981 tggcgaccga atcgccatgc gtttgcgatt gcccgatcag gctgcctcaa tgttcatcga
     2041 aactgccacg gttcggtggg ggaaagaaca aacgtacgga gtggaatttg agaggttatc
     2101 tcccaacgac aacaagcatc tccagagctt catgaaccac caatcccggt cgggccagtc
     2161 tcttgccgtc tgactttcca accttgcata gtatttctga acacttgaag gcgttcaacg
     2221 gtcattgtct tcgcgtagag agacgaactg tttacgaaag gtgacaacct tcacgagata
     2281 atccctcgtc tcctggtacg gcagattatt acgcaatcgg tcatagacgg ccggtggttg
     2341 gagacggttg atttgattga gagcggctac ggagtcgccg gcaaacgtct tgaagacatt
     2401 ccgagggcct gtgttatagg ccgagatcac gcaatactct cgcgaaacat cattgtcgat
     2461 ctgctcgagc agattatacg acaggacatt gaggtaggcg ctgccgagtt cgatattatt
     2521 ctccgggtcg aacaggtaat cgcgcgatgg aatcgtatct tttcccttga ctcttcgata
     2581 ggcatcccgc cctccactgg tgggaaccag ctgcatcaaa ccataggccg gggcggaact
     2641 cacggcaaag ggattgaagt tgctttccgt cctaatgacg gcaaagatga ggctggga
//
LOCUS       DASTWE010001102         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1289664, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001102 DASTWE010000000
VERSION     DASTWE010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289664"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..742
                     /locus_tag="VFT30_11485"
     CDS             281..742
                     /locus_tag="VFT30_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093300.1"
                     /translation="MFPSVAQATHEADHRFMVEGYVCGQDGKGLSNTEVLVKDTRISY
                     GQVVRTDGDGYYKAMFHLHNDNLGDPLLVEARGEQQNHKIQFDPKDLESERKIKVNFG
                     TGCDASGPPVWIWWGGGAMVAAAGTLIGMKLVRSQRKQERGKGKSQRQRKT"
     gene            966..1445
                     /locus_tag="VFT30_11490"
     CDS             966..1445
                     /locus_tag="VFT30_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5093301.1"
                     /translation="MRISSIHAVWILSFGLLSSCGGEQGEGPIIPPPPAPAEYADKHM
                     PADWWGDAQKLEEGRKLFLGETNPDVNCASCHGKDGKPVKAGATDFRNPERMKLYSDS
                     VWFWRISEGVPNTKMKGWKSKLSDEDRWKLVLYERNFGLSGKTWNEEKKQWVDAGMK"
     gene            1537..>2849
                     /locus_tag="VFT30_11495"
     CDS             1537..>2849
                     /locus_tag="VFT30_11495"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU5093302.1"
                     /translation="MKKLSSSVLALVVLFSLCIAATSVYAQEIGAPTVEFPYTGNRTA
                     VWIVAQLHILFAAFILGAPIFVVISEWLGYRKQDPRYDRLAKEITKVTVILFSMTAVT
                     GGLFIFVLLAAYPQFTTSFINQFYMVFAVLYPALFIAETILMYAYLYTWDVWKGEKKG
                     RHIALGVLLNLVCLLILFVINGPTSFMNTPPKAEGVSPQDFIAAATLWDKIANQSWFP
                     LSLHRIDGNVAFGGFIAGMIAAYMYMGAKTQEERAYYDWMGFAAIWIAVGGSLLQPFT
                     GLLLAYEMCDYDFSFCPYMMADQLSMFFETQGIMIGLLFLGINYYSWLSVKRIEGAEN
                     VRITILAPIVLVVLLPVTMWVMDKYWIPDPMSLVFLLPLTLSPFLLGRFIPKTVSART
                     VIKIGFIVMLVSDAVWLTPGGFVATGTDMPDELKLPEGWDYLSSMP"
BASE COUNT          638 a          655 c          835 g          721 t
ORIGIN      
        1 gcacgcaagg ctacctctga agctgttgta ggcctcaggc gatttggaga gtagaacggc
       61 atcgaaagat tgctctgcat cagcaaaccc gcctgccttt tcgttgacaa agatttggcg
      121 ctgtgctaga aacccctgcc ctaaatgtag cgtgggcgtt gtactgccgg cgcgcatttc
      181 ttcgaaggag cgagcgacga gtcagcgatc tagttcgaaa cttagcagcc gaagtccgac
      241 aggacagtta atcctagctt gctgtctcgt aatgaacgtt atgttcccat ccgtggctca
      301 agccacacat gaagcggacc atcgtttcat ggtggagggg tatgtttgcg gacaggacgg
      361 gaaggggctt tcgaatacag aggttctcgt caaggacacc agaatttcat acggtcaggt
      421 tgtacggacg gatggagatg gctattacaa ggcgatgttt catctgcaca acgacaatct
      481 tggcgatccc cttctggtag aggcgagggg ggagcagcaa aaccataaga ttcaattcga
      541 cccgaaagat cttgagagtg aacgaaagat caaggttaac tttggaaccg gctgcgatgc
      601 gagcggtcct cccgtctgga tttggtgggg gggtggagcg atggtcgcag cggctggtac
      661 tctaatcggg atgaaacttg tccgttctca acggaagcag gaacggggca aggggaaatc
      721 gcagagacag agaaaaacat aatcgccgat gacgggcctt aggggctgcg gggtgcctcg
      781 ccgaatcggt cgataggtga ggcgagggcc gttgtgcaga gtgtcgagaa agggtgtagt
      841 ggaacgcttt tgttagaggt ggtggcacct cagcagaggc gtttttttgt ttgtctgtcc
      901 tttcttgagg ctgttacagt ctcgacgaga atttctcaac gacagagtga tagatcgtaa
      961 tgaatatgag gataagctcg attcacgctg tatggatttt gagttttgga ctattgtcga
     1021 gttgcggtgg agagcaggga gaagggccga ttattcctcc gcctccggct ccggccgaat
     1081 atgccgataa gcatatgccg gcagactggt ggggggatgc tcagaagctg gaagagggac
     1141 gtaagctgtt cctcggcgag acaaatcccg acgtaaactg tgccagctgc catgggaaag
     1201 acggaaagcc ggtcaaggcg ggtgctacgg atttccgaaa ccccgagcgt atgaaactct
     1261 actcggattc cgtctggttt tggcgtattt ccgaaggagt tccaaatacc aagatgaaag
     1321 gctggaagag caagctttca gatgaggatc gatggaagct ggtgctctat gaacggaatt
     1381 ttggactgtc gggaaagact tggaatgaag agaagaagca gtgggttgat gccggcatga
     1441 aatgattagt ccaagtttgt aggtgattat ctcgggtaac tgtagtaaac gctgaaaaca
     1501 aaaccctagg ttctaaagta ggggcatcga ggcatcgtga aaaaactgtc gagcagtgtc
     1561 ctagctctcg ttgtgttgtt ctcgctatgt atagctgcca ccagcgtcta tgcccaggag
     1621 attggggcgc ctacggtgga gtttccgtac acgggtaatc ggacggctgt atggatcgtc
     1681 gctcaactcc atatcttatt tgccgcattt atcctcggtg cgcccatctt cgtcgtcatt
     1741 tcagaatggt tgggttatcg gaagcaggac ccccggtatg atcgcttagc taaagagatc
     1801 accaaggtga cggtcatcct cttcagtatg acggccgtga cgggcggctt gttcattttc
     1861 gttcttctgg ccgcctaccc acagtttacg acctcattca tcaatcagtt ttatatggtg
     1921 ttcgcagtat tgtatcctgc gttgtttatc gctgagacca tcctcatgta tgcctacctc
     1981 tacacctggg atgtctggaa gggtgaaaag aaagggcgtc atattgctct cggcgtgctc
     2041 ttgaatcttg tttgtctgct catcttattt gtgatcaacg gccccacgtc gtttatgaac
     2101 acgccaccga aggccgaagg ggtgtcgccg caggatttca tcgcggcagc aaccttgtgg
     2161 gataaaattg cgaaccaaag ttggttcccc ctgagtctcc accgaatcga tgggaacgtg
     2221 gcattcggtg ggttcatcgc cgggatgatc gccgcctaca tgtacatggg ggcgaagaca
     2281 caggaggagc gagcctacta cgattggatg ggcttcgccg ccatctggat cgcggtgggt
     2341 gggtcgctct tgcagccgtt cacgggactc ttgttggcgt atgaaatgtg cgattacgat
     2401 ttttcattct gcccgtacat gatggccgat cagctctcca tgttttttga aacgcagggc
     2461 atcatgattg ggctgctgtt cttgggcatc aactattata gttggctcag tgtcaaacga
     2521 atcgaagggg ctgaaaacgt tcggatcacg atcctggctc ccattgtcct cgtggtcctc
     2581 cttcctgtca ccatgtgggt gatggataaa tattggattc cagatccgat gtcgttggtg
     2641 ttcctccttc ccttgacgtt gtcgccattc ttattgggtc gattcattcc caagactgtc
     2701 tccgcgcgga ccgtcatcaa gatcggcttt atcgtcatgc tcgtaagcga cgccgtctgg
     2761 ctgacccccg gtggattcgt ggccaccggc actgatatgc ccgatgagct gaaattgccg
     2821 gaagggtggg attatttgtc gtcgatgcc
//
LOCUS       DASTWE010001103         1327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1128631, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001103 DASTWE010000000
VERSION     DASTWE010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128631"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFT30_11500"
     CDS             complement(<1..343)
                     /locus_tag="VFT30_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5093303.1"
                     /translation="MRTILVVDDEPTARVALAARLKRLGYRVIEAGDGKAGLEALRRE
                     RPDLTILDWMMPEMDGPSFCEQVRRDPELLSSQVLMMTSHDQPEQIAEGLARGADDFL
                     SKAASKYEITAR"
     gene            complement(463..813)
                     /locus_tag="VFT30_11505"
     CDS             complement(463..813)
                     /locus_tag="VFT30_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5093304.1"
                     /translation="MSMQTKERPVPNGVILEMTGDLTYANREQFKTAVETIRQKGCRH
                     LILNMAEVRFVDSSGLGLLALIAQNFKLSQGKVSMLKPQSYVREIMSLVNIQKLIPVY
                     DNEQDALAGHQRAA"
     gene            complement(900..>1327)
                     /locus_tag="VFT30_11510"
     CDS             complement(900..>1327)
                     /locus_tag="VFT30_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEU5093305.1"
                     /translation="LNNLCRIAVKEAQDGDSVLPGHALIAPGSYHMTLVRSGARYTVH
                     INQDPPVNRHRPSVDVMFASVARYAGANAVGIILTGMGGDGAKEMLTMKQAGAFTIAQ
                     DEASCVVFGMPKEAIKAGAVDKVLPLDDIADAILAHVSR"
BASE COUNT          267 a          393 c          347 g          320 t
ORIGIN      
        1 cgcgagcagt aatttcgtac ttgctagcgg ccttgctgag aaagtcatcg gctccgcgag
       61 ccaggccttc ggcgatctgc tcaggctgat catgactcgt catcatgaga acttgactcg
      121 acagcaattc aggatcccga cgaacctgct cgcagaaaga gggtccgtcc atttctggca
      181 tcatccaatc caagatggtc agatcgggcc gctcgcgtcg gagcgcttcc aacccggctt
      241 ttccatctcc ggcttcaatc acacgatagc cgagccgttt gagtcgagcc gccaaggcca
      301 ctcttgcggt gggttcatca tccacgacca ggatggtacg catccctacc tcgacgccat
      361 tgcaaagtgt cgtcggagtc gacctcgtat gagacgtaac gagaacacct gtgagcgttt
      421 caacggcgaa cgatccctct gtctgatcag tttgtagtcg gcctaggctg cacgctggtg
      481 tccggccaac gcatcctgct cattgtcgta gacggggatc agtttctgaa tattgaccag
      541 actcatgatt tcccgaacgt aactttgcgg ctttaacatg ctcacttttc cttggctcag
      601 cttgaagttc tgggcgatga gcgccagcaa gccgagcccg gagctgtcaa caaatcggac
      661 ttcggccatg ttcaagatca gatgccggca gcccttctgt cgaattgtct ccacggctgt
      721 tttgaattgt tcacgattcg cgtacgtgag atctcccgtc atctcaagaa tcacgccgtt
      781 cgggacgggg cgctctttcg tttgcatcga catgggtagc tccttcatgt gataaggcga
      841 aatggagtcg atctttctac gcagcctgat ggtatgatcg gcatacagac cgtgattcct
      901 cagcggctca cgtgcgcgag tatggcgtcg gcgatgtcgt ccaacggcag aactttgtcc
      961 acggctcccg ccttgatcgc ttccttcggc atgccgaaca cgacacagct cgcttcatct
     1021 tgagcgatcg tgaacgcacc agcctgtttc atcgtcaaca tttctttggc accatcgcct
     1081 cccatcccgg tcaggatgat tccgactgcg ttggccccgg cgtagcgggc tactgaagca
     1141 aacatgacat ccacagacgg tcggtgacga ttgaccggag gatcctgatt gatgtgcact
     1201 gtgtatcggg ccccacttct gaccagtgtc atgtggtagc ttcccggcgc aatcaaggca
     1261 tgccctggca acacactgtc tccgtcctgc gcttctttga cggcaatccg gcagagattg
     1321 ttcagcc
//
LOCUS       DASTWE010001104         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1096099, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001104 DASTWE010000000
VERSION     DASTWE010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096099"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFT30_11515"
     CDS             complement(<1..246)
                     /locus_tag="VFT30_11515"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5093306.1"
                     /translation="MSVLLHHGKRLFCLALFASALSAQTTLGTITGLVSDNTGAIIPN
                     AVVVATNVNTGSKGQTVSSATGNYVIPSLQVGTYEVSV"
     gene            416..>1283
                     /locus_tag="VFT30_11520"
     CDS             416..>1283
                     /locus_tag="VFT30_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093307.1"
                     /translation="MAGRVSQVDQAAAITRVVQVSAQAPAFREHIGRITTSAAFKGSR
                     RSQDFLTHIVEQALAGHFDALKERALGVELFGRNPSYDTGEDAIVRVTASDVRRRLQQ
                     YYGQTPSEWRIELPAGSDIPEFHKSDTAVETRREETLPAESASVRRSRSKLWYIVAGI
                     GILGLLAVAFWVGRPPYRVSAVQTPPWSAMLQNNRPIRILLCDPDISTLQQLLDFNIS
                     LPDYANKEFIPKSLANLPRTREYSSFLRGVNVAAVDTGLTLAVSELAVQNQRHVRTQT
                     ARSIQISDLKTED"
BASE COUNT          297 a          348 c          350 g          288 t
ORIGIN      
        1 aacggacact tcatatgtac ccacttgcag gctggggatg acatagttgc cggtggccga
       61 actgacggtt tggcccttcg aaccggtatt gacattcgtg gccaccacaa cggcgttcgg
      121 aataatcgct ccggtattgt cactgaccaa ccccgtgatc gttcccagag tggtttgtgc
      181 ggacagagcc gacgcaaaca aggcgagaca gaatagacgc ttcccgtggt gaagcagaac
      241 ggacatggtt tcctcctgaa ggcagccggg gacgactata tgccaatact ggctgattta
      301 acaacgctaa aaacgtttct aaccttcttc taaattattg gatttatggc gataatttct
      361 ttacaagatt gtttcggtag ctacgctgtg atatcgtgcc tctcgcgtag gggagatggc
      421 aggcagagtc agtcaagtcg atcaggcggc agcgatcacg agggttgtcc aggtttcagc
      481 ccaggccccc gcctttcgtg agcacatcgg cagaatcaca accagcgcgg ccttcaaggg
      541 aagccggcga agccaggatt tcctgaccca catcgtcgag caggcgctgg cgggtcattt
      601 cgacgcgctg aaggaaaggg ctcttggggt ggagctgttc gggcgaaatc cctcttacga
      661 cacgggcgaa gacgccatcg tccgagtgac ggccagcgat gttcgccggc gattacagca
      721 atattacggt caaacgccat cggaatggcg aattgaactt ccagccggat cagacatccc
      781 ggaatttcat aaaagcgaca cggccgtcga aactcgccgc gaggaaacgc ttccagcgga
      841 atccgcctca gtccggcgta gccgatcgaa actttggtac atcgtagcgg ggatcggcat
      901 cctcggtctg ttggcggtgg cgttttgggt gggaagaccg ccctatcgtg tgagtgccgt
      961 tcagaccccg ccgtggtctg cgatgctgca gaacaatcgt ccgatccgaa ttcttttgtg
     1021 tgatccggat atttccactc tccagcaact tctggatttc aatatttctc tgccggatta
     1081 tgcgaataag gaatttattc caaaatccct cgccaatctt cctcgtaccc gcgaatattc
     1141 gtctttcctt cggggcgtaa atgtagccgc cgtggatacg ggactgactc tcgccgtttc
     1201 cgagctcgct gtacaaaacc agcgccacgt gagaacccag accgcccgaa gcatacaaat
     1261 ttcagatctg aagacggaag ata
//
LOCUS       DASTWE010001105         1242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_869815, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001105 DASTWE010000000
VERSION     DASTWE010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869815"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..616)
                     /locus_tag="VFT30_11525"
     CDS             complement(170..616)
                     /locus_tag="VFT30_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093308.1"
                     /translation="MFAAQPQRSISDAFDPVTVWVVNSDAQVRRLVDNAVQEYPLHLD
                     LCVIFSEDFRSAVAAPPPAVVLINLTAPTESCFNLLPEVRQQWPDAHVIFLSHSDDIH
                     HWAEAIQLGAYEFLPRSVECDQLGWVLQSALWTRRRKMAKHPARPV"
     gene            complement(799..>1242)
                     /locus_tag="VFT30_11530"
     CDS             complement(799..>1242)
                     /locus_tag="VFT30_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093309.1"
                     /translation="LDAVLSGLSLDPRLQCITIVKEYRCDPVLRADPNLLERLFLNLT
                     LNAVQAMKFSGELKIRVVETSQTVLISFIDKGCGMDRGVQEKIFGPFFTTKAAGTGLG
                     LFLCKKYAEAYEGSIEVTTELGLGSTFTVSLPRVPQIAEVAREPL"
BASE COUNT          297 a          347 c          324 g          274 t
ORIGIN      
        1 accgtttgca tagaaccatc caaccagcgg atcttcgccc attgccggac ttcagctcaa
       61 acatctgcgg gtctggaact catcctttgt cgctaccagc ctgttgctga aatccggtta
      121 atttacgccc ataactcgcg caccatgggc gattcctccc ggcacggttt cagactgggc
      181 gagctggatg tttcgccatt ttcctccttc tcgtccaaag agcgctctga agaacccatc
      241 ccaattgatc acactcaacc gatcttggca gaaactcgta ggcaccgagt tgaatagctt
      301 cagcccaatg atgaatgtcg tccgagtggg acaggaagat cacgtgggca tccggccatt
      361 gctgccgaac ttcaggaaga agattgaaac aggactcggt tggtgcagtg agattgatca
      421 gaacgaccgc cgggggagga gctgcgacag ccgatctgaa gtcctctgaa aagatcacgc
      481 acaggtcgag gtggagcggg tattcctgaa ctgcgttgtc cacgagtcga cggacctgcg
      541 catcggaatt gaccacccac accgtcacag ggtcaaacgc gtcgcttatt gaacgttgtg
      601 gttgggcagc gaacataaga acctctgaac ttgagtcacg aaccatccca tttactttag
      661 aacgccagtc tcgcagtcac ctcgactcca ggccgtgaac agcttcgaga gtcgaacacc
      721 gtgatcctcg atgatgtctg gccctgaccg aggtttccac acgagtacca atgtttggcg
      781 atcgatctca cagagaagtt agagcggctc tctggccact tcggctattt gagggactcg
      841 gggtagagaa acggtaaaag tagacccgag gccaagctct gttgtgactt caatgctgcc
      901 ttcgtatgcc tccgcatact tcttacacag gaataggccc aggccagttc cggccgcctt
      961 ggtggtgaag aagggtccaa aaatcttctc ttgaacgcct ctatccattc cacagccttt
     1021 gtcgatgaac gaaatcaaaa cggtctggct ggtttctaca actcggattt tgagctcccc
     1081 ggagaatttc atggcctgga ccgcgttcag ggtgagattc aggaacaggc gctccagcag
     1141 gttggggtct gccctcaaca caggatcgca gcgatactcc ttgactatcg tgatgcactg
     1201 cagccgcgga tccagcgaaa gcccggaaag aacagcatcc ag
//
LOCUS       DASTWE010001106         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1419599, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001106 DASTWE010000000
VERSION     DASTWE010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419599"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFT30_11535"
     CDS             <1..379
                     /locus_tag="VFT30_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093310.1"
                     /translation="VLVVAFPSRSIAQEATEPKSIRMTCGACPDGYAMTGVTSSPEIC
                     KDGDPTLVQCVPLGANMLAVCGSCPDSYAEIGSSSVPARCGTKDGGRLTQCQLRKMEH
                     NFPDSTQGYKRCPPDCGSTAQPG"
     gene            447..>1015
                     /gene="ruvB"
                     /locus_tag="VFT30_11540"
     CDS             447..>1015
                     /gene="ruvB"
                     /locus_tag="VFT30_11540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5093311.1"
                     /translation="MTERFVTNRATDEERGQENVLRPQTLNEYVGQDKMKESLRICIE
                     AAKQREEALDHAIFYGPPGLGKTTIAHIIAKEMGATLRSTSGLVLTHAGDLAAILTNL
                     QEHDVLFIDEIHRLPASVEEALYPAMEDFQLDLVIGQGPATRTVKLDLPPFTLVGATT
                     RAGSLTSPLRDRCGLVYRLEFYEPSELEA"
BASE COUNT          233 a          270 c          297 g          215 t
ORIGIN      
        1 tgttctcgta gtcgcgttcc cttctcggag tatcgcgcag gaggccacgg aaccgaaaag
       61 tatcagaatg acctgcggtg cgtgtccgga tgggtatgcg atgaccggcg tcacctcgtc
      121 tccggaaatc tgcaaagacg gagatccgac tctcgtgcaa tgtgtgccgc tgggggcaaa
      181 catgctggct gtgtgcggat cctgtcccga cagttatgct gaaatcggaa gttcctcagt
      241 ccctgctcgg tgcgggacca aagacggtgg ccggctgaca caatgtcagt tgaggaaaat
      301 ggaacacaat ttccccgact cgactcaggg atataaaaga tgtcctcctg attgcggcag
      361 tacggctcag cctgggtaag gggctctgcc gcctcctccg aagtatcaac agatgccgga
      421 aagcaagtag acatgggcgt cgcatcatga cggaacggtt tgtcaccaat cgcgccacgg
      481 acgaagagcg gggtcaggag aatgtgcttc gacctcagac gctcaatgaa tacgtcggcc
      541 aagacaaaat gaaggaatcg cttcgaattt gtatcgaagc ggccaagcag cgggaagagg
      601 cactcgacca cgccatcttc tatggaccgc ccggtctcgg aaagacgacc atcgcccata
      661 ttatcgctaa ggagatgggc gcgacattgc ggtcgacgtc agggctggtc ttaacccatg
      721 ccggcgattt ggcggcgatt ctgaccaatc ttcaggaaca tgatgtactc tttatcgatg
      781 aaatccaccg cctgcccgca tctgttgaag aagcgttgta tccagccatg gaggactttc
      841 agctggatct ggtgattggg cagggacctg ccaccagaac cgtcaaactt gatttgcctc
      901 cgttcacact tgtgggggcc acgaccagag ccggttcact gacatccccg ctgcgggatc
      961 ggtgtggatt ggtctatcgg ctggagtttt atgaaccatc cgaactggag gcgat
//
LOCUS       DASTWE010001107         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_223378, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001107 DASTWE010000000
VERSION     DASTWE010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223378"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1094)
                     /gene="catE"
                     /locus_tag="VFT30_11545"
     CDS             complement(<1..>1094)
                     /gene="catE"
                     /locus_tag="VFT30_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase C"
                     /protein_id="HEU5093312.1"
                     /translation="NIPVFFIQDAIKFPDIIHAVKPEPDRAFPQAASAHDNFWDFISL
                     TPESMHMIMWVMSDRAIPRSFRFMEGFGVHTFRFLNARDESTFVKFHWKPKLGLQSVV
                     WNEAVKINGADPDFHRRDLWNAIQSGNFPEWELRVQLFDQEFADNFDFDVLDPTKIIP
                     EEILEPIPVGRLVLDRMPDNFFAETEQVAFMTQNVPPGIDFSNDPLLQGRNFSYLDTQ
                     LKRLGSPNFTHIPINAPKCPFAHFQQDGHMAMRNPVGRVNYQPNSFAEGPRESQQRGF
                     RSFPDVEQGQKARLRAESFADHYSQARQFFNSQTEPEQRHIAMALTFELSKVDTLVIR
                     ERMVAHLLNIDEGLAETVAEKLGMKELPKP"
BASE COUNT          231 a          283 c          343 g          237 t
ORIGIN      
        1 aggtttgggt aattccttca tgccgagctt ttcggcgact gtttctgcca aaccttcatc
       61 gatattcagg aggtgagcta ccatgcgctc acggatcacg agcgtgtcca ccttcgacag
      121 ttcgaacgtc agtgccatag cgatgtgacg ctgttctggc tctgtctggc tgttgaagaa
      181 ctggcgcgcc tggctgtagt ggtcagcgaa gctctcggcg cgcaagcggg ccttctggcc
      241 ctgttcgaca tcggggaacg atcggaaccc acgctgttgc gattcccggg gaccttcggc
      301 gaacgagttg ggctgatagt tcacgcgacc cacagggttg cgcatcgcca tgtgcccgtc
      361 ttgctggaag tgagcaaatg gacacttggg tgcgttgatc gggatgtgcg taaagttcgg
      421 actgccgagc cgcttcagtt gtgtgtccag gtaggagaag ttgcgaccct gaaggagtgg
      481 atcgttggag aagtcgatgc cgggtggcac gttctgcgtc atgaacgcaa cttgctcggt
      541 ctcggcgaag aagttgtcgg gcatccgatc gagaaccaag cggccgacag ggatgggctc
      601 gagtatttcc tcgggaataa tcttcgtagg atcgagcacg tcgaagtcga agttatcggc
      661 gaactcctgg tcaaacaact gaacgcgcaa ctcccattcg ggaaagttgc cggactggat
      721 agcgttccac agatcgcggc ggtggaaatc gggatcggct ccgttgatct tgacggcctc
      781 gttccagaca acagactgaa gccccagctt cggcttccaa tgaaacttca cgaaggtcga
      841 ctcgtccctg gcgttgagga accggaaggt atggacgccg aaaccttcca tgaaacggaa
      901 cgaccgaggg atcgcgcggt ccgacatgac ccacatgatc atgtgcatcg actcgggcgt
      961 cagggatatg aagtcccaga aattgtcatg tgccgaagcc gcttgcggaa acgcccggtc
     1021 gggctcgggc ttaacggcgt gaatgatgtc gggaaacttg atcgcgtcct ggatgaagaa
     1081 caccgggatg ttgt
//
LOCUS       DASTWE010001108         1154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_29871, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001108 DASTWE010000000
VERSION     DASTWE010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29871"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1154)
                     /locus_tag="VFT30_11550"
     CDS             complement(<1..>1154)
                     /locus_tag="VFT30_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEU5093313.1"
                     /translation="LLSANVTYGVTALGWREFATVVIGSGEGNLSLEQAIEGLLEGVV
                     SALKRLGGQGRLDILRVVEYDKDRFKAILKILEKIKNNGDGSLTLQLTTRELSKRIHR
                     KKPSKPEHLDTMKGSRITIERDRDVFRFSAIREQAVIPVREITIQSSYAEGASELLKE
                     SRSVEDQQKYGKLLHNYLMPEDFQDMVDVTPLTLMVDRSTAAFPWEMAAFEQHGKAIF
                     YGPGRQLTRQFRTILSGPPGLIASPIPNRLRVLVIADPAPEAELQLSGAREEGRAVVH
                     IIQNMKEEQGLDITIEQRIGANECDPVELLALILSEEFDLIHYSGHGDFDEKNPSKSG
                     WIFGKEHVLSARDIFRARRVPRLIFANACLSGVVRSGKPFTLQESNRNLA"
BASE COUNT          246 a          342 c          294 g          272 t
ORIGIN      
        1 cggcaaggtt tcgattcgat tcttgcaatg taaacggctt tcccgaacga acgaccccgg
       61 acaaacaggc attggcaaag atcaggcgcg gcacccgtcg cgcgcggaaa atatctctgg
      121 ctgaaagcac gtgttccttg ccgaagatcc agccactctt ggagggattc ttctcgtcga
      181 agtcgccatg cccagagtaa tgaatcaggt cgaactcctc gctcaggatc agcgcgagca
      241 gttcgaccgg atcgcattca ttcgcaccga tacgttgctc gatcgtgata tccagtccct
      301 gttcttcctt catgttctgt atgatgtgta caaccgcgcg cccctcttcc cgtgctcccg
      361 acaattgcag ctcggcttcc ggagccggat cggcgatgac cagcacacga aggcggttcg
      421 gaatgggcga agcaataagc cctggcggac cggacaagat ggttcggaat tgtcgcgtta
      481 attgccgccc cggtccgtag aaaatagctt tcccatgctg ctcgaatgcc gccatttccc
      541 atggaaatgc cgctgtcgaa cggtcgacca tgagcgtgag cggtgtgaca tcgaccatgt
      601 cttgaaagtc ttccggcatg aggtagttgt gtaaaagttt cccatacttc tgctgatcct
      661 ctacgcttcg cgattccttc aacagttcgg aggctccttc cgcataggag gattgaatcg
      721 tgatctctcg aactggaatc accgcctgct ccctgatagc ggagaaccgg aatacatcac
      781 gatcccgctc aatcgtaatg cgactcccct tcattgtgtc caggtgctcc ggcttcgacg
      841 gtttcttgcg atggattcgc ttcgagagtt cccgcgtcgt gagctgcaat gtcagcgagc
      901 catcgccatt gttcttgatc ttctccaaga tcttcagaat agccttgaag cggtccttgt
      961 catactctac gacgcgcagg atatcgagac gtccttgccc tccgagtcgt ttgagtgccg
     1021 ataccacccc ctcaagcaga ccttcgatcg cttgctctaa agacaaattg ccttctccgg
     1081 acccgataac cacggtggcg aactcacgcc acccgagcgc ggtcactccg taagtgacat
     1141 tggcggacag gaga
//
LOCUS       DASTWE010001109         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_256203, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001109 DASTWE010000000
VERSION     DASTWE010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256203"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFT30_11555"
     CDS             <1..612
                     /locus_tag="VFT30_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5093314.1"
                     /translation="RQEIVIKPLGPLKPLEQSFFGGATIDPEGGVVLVIDPSRLTSRE
                     TRESAARPLFSKTTPLPEESLAQEKMLSDKPQKARLLLIDDSLSIRKFVGRMLESAGY
                     PFDTAVDGDDGLRKASTTNYQMILTDLEMPKLNGFEVIQALRSRPETRHTPVVVMTTR
                     ARDKHRQMAISLGANSYISKPVEERMLLHEVERWIGKAPALRK"
     gene            683..1201
                     /locus_tag="VFT30_11560"
     CDS             683..1201
                     /locus_tag="VFT30_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU5093315.1"
                     /translation="MNVREPMTGSPSPTFLSRFLIVTLGGRYLALSAESICGLLTPEE
                     AGNGENQTAHGMVYGAINLTDRLSLPNDQGRANTRVVLLSERETRGCIRVTTVHGLLE
                     LQQSQILPLPAQFRGPERYWYRGMILFANSIALALNTTWVLDEQVSDPEGSEGHGCTL
                     EPVAAPKILGER"
     gene            1208..1654
                     /locus_tag="VFT30_11565"
     CDS             1208..1654
                     /locus_tag="VFT30_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU5093316.1"
                     /translation="MLRTSHKHQWETARQSWQLLVFSVGGRRLAVKTLDVSDISQWNE
                     SVPVAGRTPFVTAVIRQGQTVLPVFDLAASLHLTVQGDSPLCLRIRHPLGEMAMCIDE
                     EIPVLHTFDPAAIRTYQGKDLPAEGSYTNGPDEILILSVARLGSTG"
     gene            1693..2889
                     /locus_tag="VFT30_11570"
     CDS             1693..2889
                     /locus_tag="VFT30_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5093317.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGLGVTLAANGEEALAYLAKE
                     QPDVVVSDVIMPDKSGYEVCAFIRGNATLATTPVLLISGIVNDEVTKQAESCRADGVL
                     KKPFQGTSLKDRVLDLIAKRQEPGPAAVAGPVFVPVAIPAEKVMTVSDQLQQSPRQEV
                     SKLKEVEEQLREERIRSEELTKRLSESTEHLARAMESEMQLAIERKRANDLQETLAKV
                     EQQVSRIPDLEAALKVEQDAVGAFKQEAANWQKASGRIAELESALHAERTAAEQLVQQ
                     LADLEQVSIKTRDVEARLASAEQRGTELHQKIEDLEVELSAERRRGAETTGQLQELER
                     VATRVREMEEPLTAEQDRNELLTRRVAEAEHAAESSTKRLEEMARKLSEIARLASQLG
                     NVRGQS"
BASE COUNT          778 a          714 c          863 g          602 t
ORIGIN      
        1 cgccaagaaa tcgtcattaa gccactcggg ccactgaaac cgttggaaca gtcgtttttc
       61 ggaggagcga ccatcgatcc agagggaggg gtagttcttg ttatcgatcc gagccgcttg
      121 acgtcaagag aaaccaggga atccgctgct cgccctctct tttccaaaac tacaccactt
      181 ccagaagagt ccttagcaca ggaaaagatg ttgtcggata agccgcaaaa ggcgcgcctg
      241 cttttgatcg acgattcgtt aagcattcgc aaattcgtcg gaagaatgtt ggagtcggcc
      301 ggatatccgt ttgatacggc cgttgatggc gatgacgggc ttcgaaaggc atcgacgacc
      361 aattatcaga tgatcctcac cgatctcgaa atgccgaagc tcaacggatt cgaagttatt
      421 caagcgctca gaagtcgtcc ggaaaccagg cacacgccgg tggttgtcat gacgacgagg
      481 gctcgggaca agcaccggca gatggcgatt agccttggtg ccaattcata catttccaag
      541 ccggtagaag aacgaatgtt gctgcatgag gttgaacggt ggattggaaa ggctccagcg
      601 ctgcgcaagt aaccttgcgg gaatactcgt atcaattgac gagatgcgac ttgttgcaga
      661 aaatggacag gaagaccttg gcatgaacgt ccgggaaccg atgacgggat ctccgtcgcc
      721 aacttttctg tcacgatttc tcatcgtgac gttaggcgga cggtacctag cgttgagtgc
      781 cgaatctata tgtggattat tgactcccga ggaagctgga aacggcgaaa accagacagc
      841 tcacggtatg gtgtatggtg ccatcaatct gaccgatcga ttgagcttac cgaatgatca
      901 ggggagagcg aacacgcgtg ttgtactgct ttctgaacga gaaacgcgtg ggtgcattcg
      961 agtcaccacg gtgcacggac ttcttgaact ccaacagtct caaatcctac cgcttcctgc
     1021 gcaattccgt ggaccggaac gatactggta ccggggcatg attttattcg caaacagcat
     1081 tgcgttggct ctcaatacaa catgggttct tgatgagcag gtatcggacc cggaaggcag
     1141 tgaggggcat ggatgcactc ttgagcctgt ggcggccccg aagattcttg gcgaacgata
     1201 gccgggcatg ctgaggacca gccacaagca tcagtgggaa acggcacgtc aatcatggca
     1261 gctgttggtg ttttcggtgg gcggaaggcg attggcagtg aagacattgg atgtatcgga
     1321 tatttctcaa tggaatgaat ctgtccctgt cgctggtcga accccgtttg tcacggcggt
     1381 cattcgtcag ggacagaccg tgctaccggt attcgatctg gccgcatctc ttcatctcac
     1441 tgtgcaagga gacagtccct tgtgtctgag gataaggcac cctctcggag aaatggcgat
     1501 gtgcattgat gaagagattc cggtcctaca tacctttgat cctgccgcga ttcggacata
     1561 ccaaggcaag gatttgccgg ccgaaggcag ttataccaac ggcccggacg agatcctgat
     1621 tctctcagta gcacgactcg ggtcgacggg gtgacgattt cgggtgttcc aggatggacg
     1681 aaaggtgcag gcatgccgaa gatcttaata gccgatgaca gcatcgcagt tcgcaaagtt
     1741 gcggagcgac tattgaccga ggctggcctt ggggtcactc tcgccgcaaa cggagaagaa
     1801 gcattggcct atctagcaaa agaacagccg gatgttgtcg tgtccgacgt gatcatgccg
     1861 gacaagagtg gatatgaagt ctgtgcattt atccgtggga atgcaaccct tgcaacaaca
     1921 cctgtactgc tcatctcagg aatcgtgaac gatgaagtaa ccaaacaggc cgagtcttgc
     1981 cgcgcggacg gagtattgaa gaaaccattt caaggtacgt ccctaaagga tcgcgtcctc
     2041 gatctgatag caaaacggca ggaaccagga ccagccgcag tcgctgggcc cgttttcgtt
     2101 cctgtggcga ttcccgctga gaaggtgatg acggtatcgg atcaactgca gcagagtccg
     2161 cgtcaggagg tcagcaaact caaagaggtt gaagaacagt tacgggaaga acggattcgc
     2221 tccgaagagt tgaccaaacg actgtccgag tccacagagc atctcgccag ggccatggaa
     2281 agcgaaatgc agttggcaat cgagcgcaaa cgtgccaacg atcttcaaga gacactcgcg
     2341 aaggtagagc aacaagtatc aagaatccct gatctcgagg cggcacttaa ggtagaacag
     2401 gatgcggtcg gtgcattcaa acaggaagca gcgaactggc aaaaggcttc cggtcgaatt
     2461 gcggagctgg agtccgcttt gcacgctgaa cggacggcgg cggagcaact tgtgcaacaa
     2521 ctggcggatt tggagcaggt gtcgatcaag acaagagacg tggaagcaag acttgcaagt
     2581 gcggaacagc ggggaaccga acttcaccag aaaattgagg acctcgaagt cgaactttcg
     2641 gcggaacgac ggagaggggc agagacaaca ggccaacttc aagaattgga aagagtggcg
     2701 acgagagttc gggaaatgga agagcccttg actgcagaac aagatcgcaa cgaattgctc
     2761 actcgacgag tggcggaagc cgaacacgcc gccgagagct ccacgaaacg acttgaagaa
     2821 atggcacgca agttaagcga aattgcgaga ttggcctcgc aacttggaaa tgtgagggga
     2881 caatcctgac ccagccaatc tgagcacaga gatggtcatg tttgttgaag ggccgaaacc
     2941 gatgtccgtt gaaaccg
//
LOCUS       DASTWE010001110         1312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_708472, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001110 DASTWE010000000
VERSION     DASTWE010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708472"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..>1312)
                     /locus_tag="VFT30_11575"
     CDS             complement(66..>1312)
                     /locus_tag="VFT30_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5093318.1"
                     /translation="AKGVETQTRKLFAVCRMRRIPVLTLINKMDLPGRPPLDLMTEVE
                     QALDIHASAINWPIGSGSDFVGIVTRADSQVQLFSKTMHGGSTKVETDTLPLAELDSQ
                     GRVSQDTMAQVNHDLELLEIAGNPFTREQFIQGEVTPVFFASALTNFGIESFLDAFVE
                     LAPSPGMRPAERDDGSELLVNPTDMPFSAYVFKLQANMNPKHRDSTAFLRICSGRFER
                     DMVVKHHRLGRDIRLARPHSLVAQERSTVEEAYPGDIIGIINPGVFAIGDTVSLTGGF
                     NFKPLPQFQPEIFARLRPTDVGKRKAFDKGLSQMAQEGTVQILRSINDQEVLIAAVGR
                     LQFDVLQYRLRQEYRVETILDALPFTCSAWLEGDPSTFKSPSASMMVKDQRDRVVVLF
                     GDQLMKTIARDRNPDHALLEMG"
BASE COUNT          277 a          374 c          360 g          301 t
ORIGIN      
        1 ggaagagaca tcttggcaac cggttgggaa ccggcggcta gcagtcaaac gacaaactct
       61 ctacactagc ccatttccaa gagcgcgtga tccgggtttc gatcccgtgc gatggttttc
      121 atgagttgat cgccgaatag gacgacgact cgatcccgtt ggtccttcac catcatcgat
      181 gccgatggtg acttgaaggt ggaaggatct ccctccagcc acgcgctaca ggtaaagggc
      241 agtgcatcca agatggtttc cactcggtac tcctggcgca gtcgatattg cagcacatcg
      301 aactggagac gaccgaccgc ggcaatcagg acttcctgat cattgatact tcgcagaatc
      361 tgtaccgtcc cctcttgcgc catctgagac aggcctttgt cgaacgcttt gcgtttgccg
      421 acgtccgtcg gtctgagacg cgcgaatatt tccggttgga attgcggtag tggtttgaaa
      481 ttgaagcccc ctgtcaagga gaccgtatca ccgatggcga agactccggg attaatgatt
      541 ccgatgatat cgccgggata ggcctcttcg accgtgctgc gctcctgcgc caccagactg
      601 tgcggtctcg ccagccgaat atcccgaccc aatcggtgat gcttgaccac catatcccgt
      661 tcgaaacgcc ctgaacagat cctgagaaag gcggtgctgt ctcgatgttt aggattcata
      721 tttgcctgca gcttaaatac ataggcgctg aacggcatgt ccgtcgggtt gacgaggagt
      781 tcggacccat catcgcgctc ggccggccgc atgcctgggc ttggcgcaag ctccacaaat
      841 gcgtcgagaa agctttcaat cccaaaattc gtgagtgcgg atgcgaaaaa gaccggggtg
      901 acttccccct gaataaactg ttcacgtgtg aaagggtttc cggcgatctc caagagctcc
      961 agatcatggt tcacctgcgc catcgtgtcc tgcgataccc tgccctgcga gtcgagttct
     1021 gccagcggca acgtgtcggt ttcaactttg gtcgatccgc cgtgcatggt cttgctgaac
     1081 agctgcacct ggctgtcggc acgggtgaca attcccacga agtcactgcc tgacccgatc
     1141 ggccagttga ttgcactggc gtgaatatcc aatgcctgct ccacttcggt catcaggtca
     1201 agcggtggac gtcccggcaa atccatcttg ttgatcaaag tcaggacagg gatccgacgc
     1261 atgcggcaca cggcgaacag tttgcgtgtc tgggtttcca cgcctttggc tg
//
LOCUS       DASTWE010001111         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_288165, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001111 DASTWE010000000
VERSION     DASTWE010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288165"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFT30_11580"
     CDS             complement(<1..258)
                     /locus_tag="VFT30_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5093319.1"
                     /translation="MELGFIGLGKMGMNMVTRLRRDQHRVVVYDRSNELIKQAESQGC
                     VGSSSLSDLVGKLSVPRVVWVMVPSGSPTEETIQAVADLLQP"
     gene            complement(396..1181)
                     /locus_tag="VFT30_11585"
     CDS             complement(396..1181)
                     /locus_tag="VFT30_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093320.1"
                     /translation="MDTPTTASLPTREELNAELLEVPEPPEQPESPPPPGFEDNVEIA
                     LRHIKGRRGGDLVGVILVGSGARRALTLHSDIDLIALVKGEADGHEILRVDDRLVDIR
                     YHGYHSVEEDLAYSLRLPSLLRKGRLLYDHDGICAKLIEKVHHRFRQGPPPASINEQI
                     RLKAECYHLLGKTQDLLDKPGTAHYLLTLFYEGCISAFFRLRGFWLVAPVDVHRFMFS
                     REPALADLAGQFLTATTLTDRLNFGRQFADLLFKDVPNPARID"
     gene            complement(1351..2382)
                     /gene="ltaE"
                     /locus_tag="VFT30_11590"
     CDS             complement(1351..2382)
                     /gene="ltaE"
                     /locus_tag="VFT30_11590"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEU5093321.1"
                     /translation="MIDLRSDTVTKPTDDMRKAMARAEVGDDVYGEDPTVNRLQDMAA
                     AMLGKRCALFVPSGTMANQLAIRSHTQPGQEIIVESKSHVVRYEQGAAGALAGVQLHW
                     VTGERGIMTAEQVEAAIRPNDPHSITTALICIENTHNAGGGTIYPLSTIEKIRALAVK
                     HGIPMHLDGARLFNAVAATTLPPTVYAQHFETVSLCLSKGLGAPVGSLLISNDQRLMD
                     RARRFRRMYGGAMRQAGILAAAGIYALERHIARLKTDHDHAKKLARLLQQIPAIQIAP
                     QHVETNIVIFDIVDEHRSPADLVAALKEQGVLINAVGGRSYRAVTHLHITDKQIDEAA
                     AVFGKVLTR"
BASE COUNT          553 a          710 c          788 g          606 t
ORIGIN      
        1 gggttgtaag agatcagcta cggcctggat ggtttcctcg gtaggagatc cggaaggaac
       61 catcacccag acgacacgcg ggacactcag tttcccgacg agatcagaga gggacgaaga
      121 accgacacag ccttggctct cagcctgttt gatcaactcg ttggatcgat catacaccac
      181 cacgcgatgc tgatcgcgcc gaaggcgcgt caccatgttc attcccatct tacctagtcc
      241 gatgaatccc agttccataa gagagctcct tggtttatcg ccgggtcaca gccaatgcag
      301 tgctcgtcga tcgatctaca agctggtacg gcggctcctc tcacgttatg tgcgaaaccc
      361 ggctttaatg cagaggtagc cgttgaatgc ttcggtcagt cgatacgagc aggattcggg
      421 acatccttaa agaggagatc ggcaaattgc cggccaaagt tgagccgatc ggtgagggtg
      481 gtggccgtca ggaattgtcc ggcaaggtcg gcaagcgcgg gctcacgcga aaacatgaac
      541 cgatgaacat ccaccggggc gaccaaccaa aaccccctca aacgaaagaa tgccgagatg
      601 caaccctcgt aaaacagcgt taataggtag tgggcggttc ctggcttgtc caggagatcc
      661 tgcgtttttc ctaataaatg ataacactca gccttcaggc gaatctgttc gttgatcgaa
      721 gccggtggcg gaccttggcg aaagcggtga tggacctttt cgatcagttt cgcacagatg
      781 ccgtcgtgat cgtacaggag tctgcctttt cgaagcagcg aggggagtcg aagcgagtat
      841 gcaaggtcct cttcgacgga gtggtatcca tggtagcgaa tatctaccag acggtcgtcg
      901 acacggagga tttcgtgccc gtcggcctct cccttgacca gggcaatcaa gtcgatatcg
      961 ctgtggagcg taagggccct ccgagctccc gatccgacga gtatgacgcc gacgaggtca
     1021 ccgccccttc ggcctttgat gtgccgtaat gcgatttcga cgttgtcctc aaaccccgga
     1081 ggaggcggcg attcgggctg ctccggcggt tccgggactt cgagcagctc ggcattcagt
     1141 tcttcacgag tggggaggct ggccgtggtc ggtgtatcca taatgtttaa acctgatgca
     1201 atgagttgta ttcggaaacc atatgaatga gggcgaagga cctccattgt tttcacctgc
     1261 ctccaacgtg ttgcatggaa cgcaacaggc aaaggtaggt gtcgctaaag cattctatgg
     1321 atggaggaat atggtgtcaa cgttgaacga tcagcgtgtg aggactttgc caaagacggc
     1381 cgcggcttcg tcgatctgct tgtccgtgat atgcagatga gtgaccgctc gatagctccg
     1441 tcctccgacg gcgttgatga gcacgccttg ctctttgagg gcggccacga gatcggcggg
     1501 agagcggtgt tcgtcgacga tgtcgaaaat gacgatattt gtttctacgt gctgtggcgc
     1561 aatctggatt gcggggattt gctgcagcag gcgagccagt tttttcgcat ggtcgtggtc
     1621 ggtcttgagt cgagcgatat gccgttccaa ggcatagatg ccggcggcgg ccaagatgcc
     1681 cgcttgacgc atcgcgcccc catacatgcg gcgaaagcgg cgtgcgcgat ccatgagtcg
     1741 ctggtcgttg gagatcagca gcgatccaac gggggctccc aaccccttcg atagacagag
     1801 ggaaacggtt tcgaagtgtt gggcgtacac cgtcggaggc aaggtggtgg cggccacggc
     1861 attgaagagc ctggctccgt cgagatgcat gggaattcca tgtttcacgg cgagggctcg
     1921 gattttttcg atcgtggaga gcgggtaaat cgtcccgccc ccggcattgt gcgtgttctc
     1981 gatgcagatc aaggccgtcg tgatgctgtg cggatcgttt ggcctgatgg cggcttccac
     2041 ttgttcggca gtcataatcc ctcgctcacc agtcacccaa tgaagttgca cgccggccag
     2101 tgcacccgct gctccctgtt catagcgaac gacatgactt ttgctctcga cgataatttc
     2161 ctgtcccggt tgagtgtgtg atcggatcgc cagttgattg gccatggtgc cggaggggac
     2221 gaaaagcgcg catcgcttgc caagcatggc ggctgccata tcttggaggc gattgacggt
     2281 cgggtcttcg ccatagacgt catctccgac ctcggcacgc gccatggctt tccgcatgtc
     2341 gtccgtcggt ttagtaacgg tgtcactacg aaggtcgatc atatgcgctg tctccctaga
     2401 attccgtacg gtctgcgcat tctaacagat ctgctacttg caggtgaaag cacacctgct
     2461 acactgcagg tccatgggcg ggccggatcg ttcaatactt actcaatggg ctcactccct
     2521 cggcgcgaag cgagaggaac tgatcccgtt ggcttgggcg ttcgtctatt ttttctgtct
     2581 cctgtgcggc tactccattc tccgtccagt gcgcgacgaa atggccatcc aaggaggatt
     2641 aaacaatctc ccctgga
//
LOCUS       DASTWE010001112         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_902617, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001112 DASTWE010000000
VERSION     DASTWE010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902617"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFT30_11595"
     CDS             <1..571
                     /locus_tag="VFT30_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5093322.1"
                     /translation="NRLDNLKFLYQMGLSIFLFDYRGYGKSQSIRPSEKGLYQDAYGA
                     YDYLTRTRKVRSERIVLFGRSLGASVAGELAVQRSASALILESPFPSIEAVAKVHYGG
                     FPSHWLLEAEFRLIDRLPQLSLPKLVIHGDKDDIIPVELGRQVFDAAKPPKEWYVIQG
                     ADHNNTYVMGGGAYFRRLAEFIRKALAAY"
     gene            complement(601..1005)
                     /locus_tag="VFT30_11600"
     CDS             complement(601..1005)
                     /locus_tag="VFT30_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093323.1"
                     /translation="MRSEHEREAHLRFVKALQHEHLTCMKPGCGGAMAIADHTPHNAR
                     IKSYEATCERCHAVEKISGKEEHQPSWDVASITLMAETHLLHDQPTCPFDDTPITFIS
                     LPNPRRKARYRLLCYYCGRHTEMNWPPPEAKR"
     gene            complement(1019..>1618)
                     /locus_tag="VFT30_11605"
     CDS             complement(1019..>1618)
                     /locus_tag="VFT30_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5093324.1"
                     /translation="QDITQRGGQALALRCDVADPVAAKHMVTQVEKEWGRIDVLINGA
                     GPYHRVNLFDETIEGWNEMFDGNLHPIFYLAQAVAPGMKARKAGRIISFSMANADQME
                     AQADVTAHYIAKAGVLILTRTLAKLLAPYGITVNVISPGFIDSGSAPPQELAAMTKRI
                     PAGYIGTVDDTVAAVRYLLSEDARYVNGANIQISGGWGI"
BASE COUNT          340 a          431 c          450 g          397 t
ORIGIN      
        1 caaccgtctc gacaacctca agttcctata ccagatgggc ctgtcgattt tcctgttcga
       61 ctatcgggga tatgggaaga gccagagtat ccgtcccagt gagaaaggac tttatcaaga
      121 tgcgtacggg gcttacgact acctcacacg aaccagaaag gttcgctcgg aacggatcgt
      181 cctgttcggg cggtcactcg gagcctctgt ggcgggtgaa cttgcggtgc aacgatcggc
      241 ctcagccctc atactcgaat cgccgtttcc ttccatcgaa gcagtcgcga aagtacatta
      301 cggcggcttt ccatcccatt ggcttcttga ggcagagttt cgattaattg atcgtctgcc
      361 acaactctcg ctgccgaaat tagttatcca tggggataag gacgacatca tccccgtcga
      421 attgggccgc caagttttcg atgcggccaa accgccaaag gaatggtatg tgatccaggg
      481 cgccgaccat aacaacactt atgtgatggg aggcggcgcc tacttccgcc gcctcgccga
      541 gttcattcgg aaggcgctcg ccgcgtactg aagttaagtg gcggtgatgg tctcgtcgag
      601 ttatctcttt gcttccggtg gcggccagtt catttcagtg tgccggccgc agtagtaaca
      661 aagaaggcgg taccgggcct tgcgacgggg attgggtaga gagataaagg taatgggagt
      721 atcgtcaaac gggcatgtcg gttggtcatg gaggagatgc gtttccgcca tcaacgtgat
      781 ggaggccaca tcccaagaag gttgatgttc ttccttaccg gaaatcttct caacggcgtg
      841 acaacgttcg caggtcgctt cgtaggattt tatgcgggcg ttgtgcggag tgtggtcggc
      901 aatggccatc gctccaccgc aacctggctt catacaagtt agatgctcat gctgaagggc
      961 tttgacgaat cgaagatgag cttcgcgttc atgttctgat cgcatagtcc tccttttatt
     1021 aaattcccca gcctccacta atttggatat tcgctccgtt cacgtaccgt gcatcctcgc
     1081 tcagcagata acgaacagct gcaacggtat cgtcgaccgt tccaatgtag ccggcgggaa
     1141 tgcgtttggt catggcggcg agttcttgcg gtggcgcgct gccggaatcg atgaaacctg
     1201 gcgaaatcac attcacggtg atgccgtacg gcgccaagag cttggctaat gtgcgtgtca
     1261 gaatgagcac tccggctttt gcgatatagt gggcggtcac gtcggcttgt gcttccatct
     1321 gatcggcatt ggccatactg aagctgatga tacgcccggc cttgcgggcc ttcatgcccg
     1381 gcgcgacagc ttgagcaaga tagaaaatcg gatgcaagtt gccgtcgaac atttcattcc
     1441 aaccctcgat ggtttcgtcg aacaggttca ctcgatgata tggaccggct ccgttgatca
     1501 ggacatcgat tcgcccccat tctttttcaa cctgtgtaac catgtgcttg gctgccacag
     1561 ggtcagccac atcgcatcgg agtgccagcg cttgtcctcc tcgctgcgtg atatcctg
//
LOCUS       DASTWE010001113         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_934686, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001113 DASTWE010000000
VERSION     DASTWE010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934686"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="meaB"
                     /locus_tag="VFT30_11610"
     CDS             <1..1011
                     /gene="meaB"
                     /locus_tag="VFT30_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5093325.1"
                     /translation="QEERTTYMAITLTQLLDKMFAGDEVALARLMTLVEREGREVGPI
                     LEAIAPRLGKAFTIGVTGPPGAGKSSVVDRLAALMRKAGKTVGVVAIDPSSPFTGGAL
                     LGDRIRMTQHYLDKGVFIRSMATRGSHGGLSRATKDVTKLLDAFGRDYVLIETVGVGQ
                     TELDVMGATDTVLVTVVPEAGDGVQVMKAGLMEIADIFVVNKADRDGANRMHTELTLM
                     LELKYSNLPHPEPGQPFWRIPVLQAQALNNIGMTELFEALERHHQYLGESGELQRRRK
                     VRRREELMSRVEYEVRRRLTTKARTDSDFAQFLDEVSDGKIDPHAASVQILESSVLRE
                     GT"
BASE COUNT          250 a          266 c          336 g          239 t
ORIGIN      
        1 caggaagaaa gaaccaccta catggcaatt acgcttacgc agttactcga caagatgttt
       61 gccggtgatg aagtagctct ggcgcgtttg atgaccctgg ttgaacgaga aggtcgcgaa
      121 gtcggcccta tcttggaggc gattgctcct cggcttggga aggcatttac cattggtgtt
      181 accggtcccc ctggggcagg aaaatctagc gtagtcgatc gtctagccgc tctgatgcga
      241 aaagccggta agacggtcgg ggtggtggcg attgatccct cgagcccctt cactggaggt
      301 gcgttgcttg gtgaccgcat tcgcatgacc cagcattatc ttgataaagg tgtcttcatc
      361 cgcagcatgg caaccagggg aagtcacggt ggtctctccc gggcgacgaa ggatgtcaca
      421 aaactgcttg acgcctttgg tcgcgactac gttctgattg agaccgttgg ggtcgggcag
      481 acagaacttg atgtgatggg tgcgacggat acggtgcttg tgaccgtcgt accagaagcc
      541 ggtgacggcg tacaagtgat gaaagccggg ctgatggaaa ttgccgacat cttcgtggtg
      601 aataaagctg accgcgatgg tgccaaccgt atgcataccg agttaacgct gatgctcgaa
      661 ttgaaataca gcaacctgcc acatcccgag ccagggcagc cattctggcg tattccggtg
      721 ttgcaggcgc aagcactcaa caacatcggg atgaccgagc tgttcgaagc gctagagcgg
      781 catcaccagt acctcggcga aagcggagaa cttcagcgtc gtcgtaaagt acgccgccgt
      841 gaagaattga tgtcacgcgt cgagtacgaa gtacgccggc ggttgacgac aaaagcgcga
      901 accgactccg acttcgctca gtttctcgat gaggtcagtg acgggaaaat cgacccgcat
      961 gcggcgtcag tccagattct ggaaagttcg gtgttgcggg aggggacgta agccgtaatg
     1021 cgtaaaacgt aaatggggag gagtgtggtt acgttttacg tttcacgttt tgtcgcgtaa
     1081 ggcggcttcc t
//
LOCUS       DASTWE010001114         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1096336, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001114 DASTWE010000000
VERSION     DASTWE010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096336"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFT30_11615"
     CDS             <1..293
                     /locus_tag="VFT30_11615"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HEU5093326.1"
                     /translation="AVGDVGARGVSAPIPECHGVARPFEFVSCLELREVLGKRAMDIA
                     RLLELVEDVALESIYCHTPSFYLRHAHPQTSYPNDFATWAARCVGSLRVSVS"
     gene            465..914
                     /locus_tag="VFT30_11620"
     CDS             465..914
                     /locus_tag="VFT30_11620"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5093327.1"
                     /translation="MLNDRAYVSLVGRVLIGLIFVMSGINKIADPQGTQQYMAAMGIT
                     TATTVLYAGAVFLEVAGGLSLLLGVWAKWGATALIVFMIPTTLIFHTNFGDQNQTIHF
                     LKNLAMMGGLLYVAVYGSGRLSVDRRAFKSDQDVESDETYLKKVVHQ"
BASE COUNT          229 a          266 c          316 g          283 t
ORIGIN      
        1 aagcggtcgg agatgtcgga gcccgcggcg tgagtgcccc gataccggaa tgccacggcg
       61 tcgcccgccc gtttgagttc gtcagttgtc tggaattacg cgaggtattg ggaaaacggg
      121 caatggacat cgcgcgtctg ctggaactgg tggaagatgt tgcgttggag tccatctatt
      181 gtcatacgcc cagtttctac ctgcgccatg cgcaccctca aacgtcgtat cccaacgact
      241 tcgccacgtg ggcggctcgg tgtgttggat cccttcgcgt atctgtctct tgaagggtta
      301 caccaagaga tcaccgccat tatcgacgac catttgagtt cctcacccca tagtctttcg
      361 cgcgtacacc gtcaatttac cggcgggcga ggccttttat atcaaacgat tgctcaacga
      421 gccggagagg atcggtgcga tcggtcgaaa aaggatggtg ccacatgttg aatgacaggg
      481 cgtatgtcag tcttgttgga cgggtattga tcggtttgat ctttgtgatg tctggtatca
      541 acaagattgc cgatccccag gggacgcagc agtatatggc ggcgatgggc atcacgacag
      601 cgacaacagt actttacgcg ggcgcagttt ttctggaagt ggccggtgga ctgtcgttgt
      661 tgcttggggt gtgggcgaaa tggggcgcca cggcgctcat cgtcttcatg ataccgacta
      721 ctttgatatt tcatacgaac ttcggcgatc agaatcagac cattcacttt ttgaagaatc
      781 tcgcaatgat gggtggactc ctgtatgtag ccgtctacgg atcaggacga ttaagcgtcg
      841 atcgcagagc ttttaagtcg gaccaggatg ttgagtctga tgaaacctac ctcaagaaag
      901 ttgtgcacca atagggatgt attcacattc ggcgatgatc atcccatgta gctcagggtt
      961 tatttagtat gatcactttg gctggtgctg attgggtggc ttgtccttga tgcgccgacc
     1021 ccaattttac tcacgtggaa atcatcggtg gtctgcgcgg gtggcgtctg aaggcgactc
     1081 ctcaattcgc tcgc
//
LOCUS       DASTWE010001115         1516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_223560, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001115 DASTWE010000000
VERSION     DASTWE010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223560"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VFT30_11625"
     CDS             complement(<1..1123)
                     /locus_tag="VFT30_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5093328.1"
                     /translation="MNELAGKHIVLVIENEAVPFDRRMWNMALSLREFGADVTVICPK
                     FGKDSEPFVVLQGIHVYRYDNVFADGSIPGYVREYVTAFFKTIWLYHRVLMKRSPVHV
                     VHVANPPDIFWPFALYLKLFGTRFIFDEHDLSPEAYLSRFNKDMNDTGMLFRIQVLFQ
                     RLCYRVANVIISTNETYRANAVSVDSSYARKTFVVRNGPDTRTFHLHPSNSSLRKGHR
                     YMAAYIGVMAIQDGVEYIVRAVDILVNKKNFKDIIVYLIGKGDDWDRLKQMADESGVG
                     DHIVFTGRIPDGPALEILSTADVCLSPDPYSPLNELSTMTKIMEYMCLGKPIVSFHLK
                     ENKYSAGDAALYVDNNNPEAFADGIIRLLNDPALASQMGA"
     gene            complement(1169..>1516)
                     /locus_tag="VFT30_11630"
     CDS             complement(1169..>1516)
                     /locus_tag="VFT30_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093329.1"
                     /translation="GWRPSPDASPLLAASGCAPFVGVDFHDHRQLFPLAMVLDCAMPL
                     SQGAVLDCLRSRRCEPRAFDITLRPGVTEAMKVSLLPLEAARRGLRWIYRRTRSAGAR
                     APQVSPKPPRVNG"
BASE COUNT          338 a          441 c          430 g          307 t
ORIGIN      
        1 atgcgcccat ttgtgaagcg agtgctgggt cgttcaaaag cctgatgatc ccgtccgcaa
       61 aggcctcggg attgttgttg tccacgtaaa gtgcggcgtc tccagccgag tacttgttct
      121 ctttgaggtg aaacgagacg atcggctttc caagacacat gtactccatg atcttggtca
      181 tcgttgagag ctcgttcagc gggctgtacg gatcgggcga cagacacacg tcagcggtcg
      241 acagaatctc cagggctggc ccgtcaggaa tgcgcccggt gaacacgatg tgatcgccca
      301 caccgctctc gtctgccatc tgcttgagcc gatcccagtc atcgcctttc ccgatcaagt
      361 agacgatgat gtccttgaag ttcttcttgt tgaccaggat atccaccgct ctcacgatgt
      421 attcaacgcc gtcctgaatc gccatgacgc cgatgtaagc cgccatgtat ctgtgtccct
      481 tcctgaggga ggaattggac ggatggaggt gaaacgtccg cgtgtctgga ccgtttctga
      541 caacaaacgt ctttcgtgcg tacgaagaat cgaccgacac ggcgttcgca cgatacgttt
      601 cgttcgtgga gatgatgacg ttggcaaccc ggtagcacag tcgctggaag agcacctgaa
      661 ttctgaacag catgccggtg tcgttcatgt ccttgttgaa ccggctgaga taggcctccg
      721 gggagaggtc gtgttcatcg aagatgaaac gcgtcccgaa caacttcaag tagagggcga
      781 atggccagaa gatgtcggga ggattggcca catgaacaac gtgcacaggc gaccgcttca
      841 tcaagactcg gtgatagagc cagatcgttt tgaagaacgc ggtcacatat tcccggacat
      901 agccagggat cgagccgtcg gcaaacacgt tgtcgtaccg atacacgtga atgccttgca
      961 gtaccacaaa cggttcgctg tccttgccga acttcgggca gatcacggtc acgtccgctc
     1021 cgaactcacg cagcgagagc gccatgttcc acatgcggcg atcgaacgga acggcctcgt
     1081 tctcgatgac aaggacaatg tgcttcccag ctaattcatt catacgtgag tgggcttgga
     1141 cacgaaacaa cctacgctca aacggtgatc atccattgac tcgaggcggc ttcggcgaca
     1201 cctgtggcgc acgcgcgcca gcggatctcg ttcgccggta aatccatcgc aaaccgcgcc
     1261 gcgccgcctc gagtggcaac agagacacct tcatggcctc ggtcacgccg ggccgcagcg
     1321 tgatgtcgaa cgcccgtggt tcacaccggc ggcttcgcag gcaatccagg accgcaccct
     1381 gcgacaacgg catcgcgcaa tccagcacca tcgcgagcgg gaacaactgt cggtgatcgt
     1441 gaaagtccac accgacaaac ggtgcacaac cgcttgcggc caggagcggt gaggcgtctg
     1501 gactcggacg ccagcc
//
LOCUS       DASTWE010001116         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_773562, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001116 DASTWE010000000
VERSION     DASTWE010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773562"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFT30_11635"
     CDS             <1..380
                     /locus_tag="VFT30_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5093330.1"
                     /translation="SLWQQVDRAMIQQELTNERELFHAYLNERPSAQEGQTWLEKLDL
                     ERVEAIEYPLEVETGPGHEFLHHPLLRGGFLDDVYECFEDQSLANRFMIEISQDISRF
                     TPLIAQRCVLSGWKRTKNDDRKN"
     gene            complement(667..>1261)
                     /locus_tag="VFT30_11640"
     CDS             complement(667..>1261)
                     /locus_tag="VFT30_11640"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU5093331.1"
                     /translation="GFGAQSLVKDVISGFFILLEDSVRVGDVVEIAGVGGLVEEVKLR
                     TIVLRDESGSIHVIPNGNINTVKNMTQLYSYYVFNIGVAYRENVDEVMALLKDIAEEL
                     RQDAEFADDILDPLEMWGVDRFADSAVIIKCRIKTKPIQQWRIGREMNRRIKNTFDAK
                     GIEMPFPHQTIYWGAPKAGTPPPLYVTSVSSNPSAAR"
BASE COUNT          310 a          357 c          313 g          281 t
ORIGIN      
        1 attcactgtg gcaacaggtg gatcgagcga tgattcagca agagctgacc aatgagcgag
       61 aactattcca tgcatatctg aacgaacgac catcagcgca ggaaggacaa acatggcttg
      121 agaaactcga tcttgaacga gtcgaggcta tcgagtatcc gcttgaagtt gagaccggtc
      181 ccgggcacga gtttctccat cacccgctac ttcgcggcgg ctttctcgac gacgtgtacg
      241 aatgttttga ggatcagtct ctcgccaacc ggttcatgat agaaatctca caagacatta
      301 gccgattcac cccactgatc gcgcagcgtt gtgtgctctc gggatggaag cggactaaga
      361 acgatgatcg caagaattga ggctgaattc cactctgaaa gctcagaatc ttaaaaaatg
      421 tctgatgaac gaaaagactg aggcatcgtc ttgaccgctc cagagcgtcg tgcgcaggga
      481 atttgcaagg tcactttcag cctttcgaaa tcatcctcga atcagagcca acctatcctt
      541 cattgagcaa cctgccttcg gccaggatgg tggagatgaa gacattggac atggccttcc
      601 ttcctccacc tcttggaggg tcccgacgac ccaaacgagg ccggtatgga ccggaacgct
      661 tggcgctcat cgcgcggccg acggattgga cgatacgctc gttacataga gcggtggcgg
      721 cgttccagct ttgggcgcgc cccaataaat cgtctgatgc ggaaacggca tctcgatacc
      781 cttggcatcg aaggtattct tgatcctgcg gttcatctca cggccgatcc gccattgctg
      841 aatcggtttg gtcttgatcc ggcatttgat gatgacggcg gaatcggcaa accgatcgac
      901 tccccacatc tccaacgggt ccaagatatc gtccgcaaat tccgcatctt gccgcaattc
      961 ttccgcaatg tccttcaaca aggccatgac ctcatccacg ttctcccggt aggcgacgcc
     1021 gatattgaag acataatagg aatagagttg ggtcatgttc ttcaccgtat tgatattacc
     1081 gttcggaatg acatgaatac tcccagattc gtcgcgcagc acgatggtcc tcaacttcac
     1141 ttcttcaacc aagccgccca ccccggcaat ctccacgacg tccccgaccc gcaccgagtc
     1201 ctccagaaga ataaagaacc ctgagatgac gtccttgacc aagctttggg cgccgaagcc
     1261 g
//
LOCUS       DASTWE010001117         1206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_449904, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001117 DASTWE010000000
VERSION     DASTWE010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449904"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFT30_11645"
     CDS             <1..613
                     /locus_tag="VFT30_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU5093332.1"
                     /translation="PIMATADFEARQLLKAYRKGLITDELFEAQMRELQTGTGNYTVN
                     GKSHASEKEMIVALLDEYRCAENFASEYLGSWNDVSNEECVKGGLRVIQRREAYHAKL
                     LEDRLRELGGTPQCTVSAGQRAKEMGVYASKDKTDAQKLQYVADQITDLPKALSFITD
                     VIDQIQDDQQTKELLRSLVQDEESSAKWLMEACQLMNGAKAAA"
     gene            726..968
                     /locus_tag="VFT30_11650"
     CDS             726..968
                     /locus_tag="VFT30_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5093333.1"
                     /translation="MPRIPLVEPEHASTEVRALYESFSGAGLPVMNVMKLFGNNVDFL
                     AALTHIAGALYNKPHLSPRYRELAYLRASQLNSCHY"
     gene            984..>1206
                     /locus_tag="VFT30_11655"
     CDS             984..>1206
                     /locus_tag="VFT30_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093334.1"
                     /translation="MLGRSVGLKDEEMAAMSDWETVSTFDDTDRLVLRYAEVLTRENR
                     VSDALYEELGARFPREELVELCMTVGLAAL"
BASE COUNT          288 a          309 c          340 g          269 t
ORIGIN      
        1 accaatcatg gcaactgcag atttcgaagc tcggcaatta ctgaaggcgt accgtaaagg
       61 gctcatcacg gatgaattgt ttgaagcgca gatgcgcgaa ctccagaccg gaacaggtaa
      121 ctacacggtg aatggcaaat cgcacgccag cgaaaaagag atgattgtgg ccctcttgga
      181 tgagtatcgc tgtgcagaaa acttcgcgtc tgagtaccta ggctcttgga atgatgtcag
      241 taacgaggag tgtgtgaaag gtggtttacg ggtcatccag cggcgcgaag cctatcatgc
      301 gaagcttcta gaagatcgtc tccgtgagct tggcggcacc ccgcaatgca ctgtctccgc
      361 tgggcagcgc gcgaaagaaa tgggagtcta cgcctccaag gacaagaccg acgcgcagaa
      421 gcttcaatac gtcgctgatc agatcacgga ccttcccaaa gctctgagtt ttatcaccga
      481 cgtgatcgat cagattcaag atgatcagca gacgaaggaa ctactccgtt cgttggtaca
      541 agacgaggag tccagcgcga aatggttgat ggaggcatgc cagctcatga atggggcgaa
      601 agctgctgcg taaggaagcc tgaaggaatc gtggacgcgg aaagcaacga gaggcgaccc
      661 tcgtccctcg ttgctcttct ctcattcctt ctttactctt gtcgctattt tagcctggag
      721 gtcacatgcc acgtattcca ctcgtcgaac ctgagcacgc ctcaacggaa gtgcgggcgc
      781 tctacgaaag tttttccgga gccggcttgc ctgtgatgaa tgtgatgaaa ctgttcggga
      841 acaatgtcga ttttcttgcc gcgctcactc atatcgccgg tgcactctac aacaagcccc
      901 acttgtcccc acgctatcgt gaactcgctt atctccgtgc ctcgcagctc aactcctgtc
      961 attattgagt gccgacccat gtgatgctcg gtcggagcgt cggactcaaa gatgaagaaa
     1021 tggcggcgat gtcggactgg gagaccgtat caacgtttga tgacacggat cggttggtgc
     1081 tgcgctacgc ggaggtatta actcgagaaa accgggtcag tgacgccctc tatgaggagc
     1141 tgggagcacg ttttccgcgc gaggaacttg tggaactctg catgacagtt ggtctcgcgg
     1201 cattgg
//
LOCUS       DASTWE010001118         1263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_999785, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001118 DASTWE010000000
VERSION     DASTWE010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999785"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFT30_11660"
     CDS             complement(<1..196)
                     /locus_tag="VFT30_11660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5093335.1"
                     /translation="MQIRADEISAIIKEKIKGFDKQVDVKETGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALN"
     gene            complement(206..745)
                     /gene="atpH"
                     /locus_tag="VFT30_11665"
     CDS             complement(206..745)
                     /gene="atpH"
                     /locus_tag="VFT30_11665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEU5093336.1"
                     /translation="MIKTTVARRYAQALFELLDQSTIEATRGMLTDLGQAIKESAQLR
                     HVVVSPAFGMEDKIAVLTTLADKMGCPPAGKAFLGQLVKKNRVGFLPEIADAFGKLVD
                     QLKRTQPVTVSSATTLPPAEQDRIRTRLRETLKREVDVTFQTDAGHLAGLQIHIGSTV
                     VDGTVRGRLRDLQVLLTRE"
BASE COUNT          285 a          342 c          340 g          296 t
ORIGIN      
        1 ggttcaacgc gatgccgtag agaccgccgg ggaattcgag catctcgccg gccatagctc
       61 cgtcgagacc atagaccttg gcgatgccgt ccccgacctg gatgacagac cccgtctctt
      121 tgacatcgac ttgtttatcg aagcctttga tcttctcttt aatgatcgca ctgatctcat
      181 ctgccctgat ctgcatggca actccttatt ctcgcgtcaa caggacttgc aaatcgcgca
      241 agcgacctcg gacggtgccg tccacaaccg tgctgccgat gtgaatctgc aatccggcaa
      301 ggtgaccggc atcggtctga aacgtcacat cgacttcgcg cttgagtgtt tcgcgtaggc
      361 gcgtcctgat tcgatcttgt tccgccggtg gaagagtcgt cgcggatgaa accgtcaccg
      421 gctgtgtccg ttttaattga tcgaccaact tgccgaatgc gtcggctatc tccggcagaa
      481 agcccacccg gttcttcttc accaattggc ccagaaatgc tttaccggct gggggacacc
      541 ccatcttatc ggcgagtgtg gtcagaacgg cgattttgtc ctccatcccg aacgcagggg
      601 agaccacgac gtgacgaagc tgagctgact ccttaatggc ctgaccgagg tcagtgagca
      661 ttccccgtgt agcttcgatg gttgattggt cgaggagctc gaagagggct tgagcgtaac
      721 gtcgcgcaac tgttgtctta atcaccgttc cagatccaca tacgttattt ggacaaacct
      781 aaagggttcg tgcgaggcac gaagcagagc tgggaaaatt agcattgaga cctatgagct
      841 gtcaatacga cacgatcgcg gtctggcatg gtactcatga atgctgtcgg caccagaaac
      901 acaatgagat attactagca gtggatcggc agagtacact ccgttccgca agaaatctca
      961 cccacaccgt cccacgcgtg gaacgtcggc ttcgtcatgg gtatggtcca acgtcatcaa
     1021 aaccactgac agccataatg tctggtgcgt gcgtggagag aataatgcgg atgcttactg
     1081 attgagtgct gactagtgat cgcgcaatcc cgataacgtg acgctcaaga aactcaagga
     1141 ggctgcgttt gggcgaccaa gcaatgcgac acgtgggatc agtagagcta ggatagctag
     1201 cttgcaatct ttgaagtagg gttttggatt cgcttccgcc tatagtgtgc tgccgccttc
     1261 atc
//
LOCUS       DASTWE010001119         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1129010, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001119 DASTWE010000000
VERSION     DASTWE010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129010"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFT30_11670"
     CDS             <1..891
                     /locus_tag="VFT30_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5093337.1"
                     /translation="NPFGLNSTVTLGIVSAVGRGHMGITQYEDFIQTDAAINPGNSGG
                     ALVNTNGELVGINTAIFSQTGGYQGVGFAVSTTMAKPIYESLVKSGKVVRGYLGVGIQ
                     DLNQDLAKSFNIKDSKGALISDVKDGPAEQAGLKQGDVITEYHGTPVEDGVALQRLVT
                     RTSVGSKVPLKVIREGRELEMTVRIGEQPEETKVAKVEKAEADSALSGLAVEDLDQET
                     AKELGLNGKRGVVVTGVAPDSGAEKAGLLPGDVIREINRQPIKSVKDFEKVSSDVKKG
                     GNVLILVNRRGNSLFLSVKV"
     gene            complement(951..1817)
                     /gene="purU"
                     /locus_tag="VFT30_11675"
     CDS             complement(951..1817)
                     /gene="purU"
                     /locus_tag="VFT30_11675"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEU5093338.1"
                     /translation="MTAQKKDSIVLLIHCQDRKGIVARVSGFIHDFGGNILDSDHHTD
                     EETNDFLMRMEFATEGFQIPADEISAAFAPIAKVYDMHYEVYPSSRRTRVGLLVSKQD
                     HCLADLLQRHRRDELHIDIPVIISNHDTCTSWADLFKIPFAVYPVTKETKPQQEQQVL
                     ALLKEHRVELVVMARYMQILTADFLARIGCPVINIHHSFLPAFIGANPYRQAYDRGVK
                     IIGATAHYATKDLDEGPIIEQDVIRVGHRDTVEDLVRKGRDLEEIVLARAVRRHIERR
                     VLVYGRKTVVFD"
BASE COUNT          453 a          496 c          613 g          445 t
ORIGIN      
        1 aatccgttcg gattgaattc taccgtaacg ctaggcatcg tgagcgccgt cggccgaggt
       61 cacatgggaa tcactcagta cgaggatttc attcaaacag atgccgccat taaccccggc
      121 aactcaggcg gcgcactggt aaacacaaat ggagagctcg tcggtatcaa caccgcgatc
      181 ttctctcaga ccggcgggta ccaaggcgtt ggttttgccg tatcgacgac gatggccaag
      241 ccgatctatg agagtctagt caagagcggc aaggttgtcc gaggctacct cggtgtcggc
      301 attcaggatc tgaatcagga tctggcgaag tccttcaaca tcaaggattc gaagggtgct
      361 ctaatcagcg atgtcaaaga cggtccggct gagcaggctg ggctcaagca gggcgacgtc
      421 atcaccgagt atcacggcac tcccgtggag gatggggtcg ccttgcagcg gcttgtgacc
      481 agaacgtcag tcggcagcaa agtgccattg aaagtgattc gcgagggtcg tgaacttgaa
      541 atgacggttc gaattggtga gcagcccgaa gaaacgaaag tggccaaggt ggaaaaggcc
      601 gaagctgact ctgccttgtc cggcctcgcc gtcgaggatt tggaccagga gactgccaaa
      661 gaactcggcc ttaatgggaa gcgaggcgtg gtagtgaccg gggttgctcc ggacagcgga
      721 gctgagaagg ccggactcct accgggcgac gttatccggg agatcaatag gcaaccgatc
      781 aagtccgtga aggattttga gaaggtctcc tcggatgtga aaaagggcgg caatgtgttg
      841 attctggtca atcggcgtgg gaattcgctg ttcctatcgg tgaaggtgta atactgcaaa
      901 ggagagaaga gggagcgggt cggatgaccg gctccccttt tcaacagccg ctagtcgaac
      961 accactgtct ttctgccata gactaggacg cgccgttcga tgtgccgccg caccgcgcgt
     1021 gccagcacga tttcctccaa atcgcgtcct ttccgcacga gatcctccac cgtatcccgg
     1081 tgtcccacac gaatcacgtc ttgctcgatg atcggcccct cgtccaaatc tttggttgcg
     1141 tagtgggccg ttgctccaat gattttgacg ccgcggtcgt aggcctgccg gtagggattg
     1201 gcaccgataa aggcgggcaa gaaggaatgg tgaatgttga tgaccgggca gccgatccgt
     1261 gccaaaaaat ccgcggtgag gatctgcatg tagcgagcca tgaccacgag ctccactcga
     1321 tgctctttca aaagcgccaa gacctgttgc tcttgctggg gcttggtctc tttcgtcaca
     1381 ggatagacgg caaaaggaat tttgaagagg tcggcccagc tcgtgcaggt gtcgtgattc
     1441 gagatgatga cgggaatatc gatgtgcagt tcgtctcggc ggtgccgttg gagaaggtcg
     1501 gccagacagt gatcttgttt ggagaccagt aagccgacac gggtccgtcg gctggacgga
     1561 tacacttcgt agtgcatgtc gtacacttta gcgatcgggg caaaggcggc ggaaatctcg
     1621 tccgcgggaa tttggaatcc ctccgtcgca aactccattc gcatgaggaa atcattggtc
     1681 tcctcgtctg tgtgatggtc ggaatcgaga atattgccgc cgaagtcatg gatgaatcca
     1741 gacactctcg cgacgatgcc tttgcggtct tggcaatgga tcaacaggac aatggagtct
     1801 ttcttctgag ccgtcatcga tcttcactcc tgtgagtgtg tggtcgtttt cagtgtagca
     1861 gatcggagta aagtcacact caggagatag attttctgac gatgcgtcca acgagatcgt
     1921 aggctgcagc atcggtgatt tcgacggtgc agaaatcgcc tggtttggga gaaggcattt
     1981 cggggtgaga atgacgtgcg ttgttgt
//
LOCUS       DASTWE010001120         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_385184, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001120 DASTWE010000000
VERSION     DASTWE010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385184"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VFT30_11680"
     CDS             <1..866
                     /locus_tag="VFT30_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEU5093339.1"
                     /translation="SLVSGLSCRIRLEHGSLNIDRISGDTDDGHLAGRVILNIPERGT
                     RWVRSMFGVSGVPIDHLFSLMDEPPRISGWMAARGVVQAEFGQDRVFHPSITSRRPIS
                     IMIERGRLVYAPVISKVLALINLPALLKGKTDLTKDGLPFDRLKLVFGIEHGIMNISE
                     FLLDSPILKISGTARYDFIDDKFDGVMVASPLGQYSDLLKSVPLFGKLFSGERQGFDT
                     AIFEVKGSAKDPSVVYLPAESLMAGAKGTAKLAFDLLVNAITLPEEAFSMAEDGSPVE
                     EDAVLEGTRGL"
     gene            complement(1042..1404)
                     /locus_tag="VFT30_11685"
     CDS             complement(1042..1404)
                     /locus_tag="VFT30_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5093340.1"
                     /translation="MQPFHCPSGSQRLARFWCPFYLLWIMVAGCALGAGSGLSDSPSD
                     GAISKEVEAKLAGDQFVGLSEILVTTEAGVVMLVGTVERAEQKARAADLARQVKGVKR
                     VKNDLEIHRTKASEAPTQ"
     gene            complement(1575..1994)
                     /locus_tag="VFT30_11690"
     CDS             complement(1575..1994)
                     /locus_tag="VFT30_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093341.1"
                     /translation="MNRIPIGIVLMGFLYLSGAVGCASEEAAKQPTSVGPSVSPATPE
                     IVSVGPSDFLKGCLAKISNESSNGARMIAEQSCQDNEKLHQGVVGTAIAKSGNRASVG
                     TQGDSLDACMARIPTDATAGQRMLAEESCKRDQLMHR"
BASE COUNT          469 a          603 c          611 g          526 t
ORIGIN      
        1 aatcactggt gagtggactg tcatgccgga ttcggttgga gcatggaagt ttgaacatcg
       61 atcgtatcag cggcgatact gatgatggtc atctcgccgg acgtgtgatc ctgaatatcc
      121 cggaacgagg tacgcgatgg gtccgcagca tgtttggcgt cagcggcgtg ccgatcgacc
      181 atcttttttc tctcatggat gaaccgccgc gaatcagcgg gtggatggcg gccaggggag
      241 tcgttcaggc ggagttcgga caggaccgtg tctttcatcc ctcaatcact agtcgtcggc
      301 cgatttccat catgatcgag cgggggcgtc tcgtttatgc gccggtgatc tccaaagtgt
      361 tggccctcat caatttgcct gcgttgctga aaggcaagac ggatctcacc aaggatggcc
      421 tgccgttcga ccgattgaag cttgtgttcg gcattgagca tggcatcatg aacatcagcg
      481 aattcctgct ggatagtcct attctgaaaa tcagcggaac agcacgatat gatttcatcg
      541 acgataagtt cgatggagtg atggtggcga gtccgctcgg ccaatattcc gaccttctca
      601 aaagtgtgcc gctgttcggg aaattatttt ccggtgagcg gcaaggattc gataccgcca
      661 ttttcgaggt taaaggatcg gcaaaagatc caagcgttgt ttatttgccg gctgaatcct
      721 tgatggcagg agcgaaggga acggcgaaat tggccttcga cctgttggtt aatgcgatca
      781 ctcttcccga agaagccttt tcaatggctg aggatggttc gcccgtagag gaagacgcag
      841 tgctggaagg aacgaggggt ctctaacagg atgctgaaaa agtctgccag ctttgttctc
      901 gcgtcgttca gaagctcaac gtaccgaaaa gcgtacgcct cgcctcttcg ctcgctgcgg
      961 ccgcgctgga cagccttttt gagcatcctg tgcggttaaa ccagggagcc ggctccatcg
     1021 gagagccgaa ccgtgccgcg gctattgcgt gggcgcctcg cttgctttgg tccggtgaat
     1081 ttcaaggtca ttcttcacgc gtttcacgcc tttgacttgg cgggccagat ccgccgctct
     1141 ggctttctgt tccgctcgtt cgaccgtccc caccaacatc acgacgcctg cttcggttgt
     1201 gaccaggatc tccgacaacc ctacgaattg atcccctgca agcttggcct ctacttcttt
     1261 ggagatagcc ccgtcgctgg gggaatcgga aagacccgag ccggccccca aggcgcagcc
     1321 agcaaccatg atccacaaca aatagaatgg gcaccagaat cgtgcaagcc tctgcgaccc
     1381 tgaaggacaa tggaatggct gcatctgttc cttacgcaat gggatgcggc gatatactct
     1441 cggttctcac cggagcctga cccggaaagc ataacacgat atcgccaagg actccgcagt
     1501 tttggtcaag ggtgatgtcc atgaaccaga tcggtgcatt caaccaaagc gattttgaga
     1561 taggttctag tctcttaccg atgcatcaac tgatcgcgct tacagctttc ttccgccaac
     1621 attcgttggc cagccgtggc gtctgtggga atacgcgcca tacaggcatc aagcgaatca
     1681 ccctgcgtcc cgacagatgc acgatttccg cttttcgcga tggctgtccc gaccacgcct
     1741 tgatgaagtt tttcattatc ttgacaactt tgttcagcga tcatcctcgc tccattgctg
     1801 gattcgttgg agatcttggc gaggcacccc ttcaggaaat ccgacggccc cacggaaact
     1861 atctcaggcg tggccggcga cacggatggg cctaccgatg tcggctgctt cgctgcctct
     1921 tccgaggcgc aacccaccgc acccgatagg tagaggaagc ccataagtac aattccaatc
     1981 ggaatcctat tcataagtgt catccctcct tcgacacaaa gagtggccag ctgctacgga
     2041 ttcctttcta tataacggag tccctctcat ggcgcaagct gcatgcgtgg acggcgagac
     2101 ttcgtcctca gttgcttcta ggcgtgaggg attcaggcgg atttcttcaa ttctgttgcc
     2161 ggcggcggaa gcagggtacg agggcgcgtc gttcgaaagt ggaccaggt
//
LOCUS       DASTWE010001121         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1258139, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001121 DASTWE010000000
VERSION     DASTWE010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258139"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..799)
                     /gene="rpiA"
                     /locus_tag="VFT30_11695"
     CDS             complement(101..799)
                     /gene="rpiA"
                     /locus_tag="VFT30_11695"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU5093342.1"
                     /translation="MTPENLDNLKKAAAHKAIEFIRDGMVVGLGTGSTAKHLLVALGE
                     QVRAGRKLRGVPTSQETAALARESGIPLIDTENRWEIDAALDGADQVDPDFNLIKGGG
                     GALLKEKIVVASAKQFIVMIDHTKQVPVLGGSFPLPIEVIPFGWGSTARKIEALTKSR
                     VVLRERNGVPFKTEAGHLIVDVHIDRIDRPGELETALNLIPGVVETGLFVGRTNVLIV
                     GTPQGVHTIHVPRK"
     gene            complement(796..>1203)
                     /locus_tag="VFT30_11700"
     CDS             complement(796..>1203)
                     /locus_tag="VFT30_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEU5093343.1"
                     /translation="YAIYEFLRDTKKNEPTWLAEKIRAGNPAAEIAQAGLQGQAEIAK
                     QALDLFASIYGAEAGNLALKALSLDGVYVGGGIAPKLITKLQDGTFMKAFLNKGRYKK
                     LMSAMPVKVIMNQQTALIGAASIAGALSLAPMP"
BASE COUNT          254 a          330 c          326 g          293 t
ORIGIN      
        1 ggattggaat ggtaggagtg tatacgctat cggagctgtc agaaggaagt gggcccgtcg
       61 gccgaaatcc aaaagaattt ctcgtctgct gaatgagcac tcatttcctt gggacatgga
      121 tggtgtgaac gccttgggga gtaccgacga tcaagacatt cgtccggccg acaaagagac
      181 cggtctcgac tacaccgggg atgaggttca gtgctgtttc cagttcaccc ggccgatcga
      241 tgcggtcgat atggacatca acgataagat gaccggcctc agtcttaaac ggaacgccgt
      301 tgcgctctcg taacacgacg cggcttttgg tgagcgcttc aatttttcgc gcagtgctgc
      361 cccacccaaa tgggatgacc tcgataggca acgggaagga ccctccgagc acaggtactt
      421 gtttggtgtg gtcgatcatc acaatgaatt gtttcgccga cgccaccacg atcttttctt
      481 ttaagagcgc tccgcccccg cccttgatca gattgaagtc gggatcgacc tgatcggctc
      541 catcaagggc ggcgtcgatc tcccaccgat tctcggtatc gatgagtgga ataccggact
      601 ctctggcgag cgcagcagtc tcttgtgacg tcggcacacc tcgcaacttc ctgccggcac
      661 ggacttgctc gccgagcgcc accagaagat gttttgccgt cgaccctgtt cccaggccga
      721 cgaccatgcc gtcacgaatg aattctatcg ctttgtgtgc ggcggctttt ttaagattgt
      781 ccagattttc aggggtcatg gcatgggagc gagcgagaga gcccctgcga tggacgcggc
      841 accgatcaag gccgtttgct ggttcatgat gactttcact ggcatggcgc tcatcagttt
      901 cttataccga cctttgttca aaaatgcctt catgaatgtc ccgtcttgga gtttcgtgat
      961 gagcttcggc gcaataccac caccgacata cacaccatcg agggagagcg ccttcaaggc
     1021 gagattcccg gcttctgcgc catagatcga tgcaaagaga tccagcgctt gtttggcaat
     1081 ctcggcttgt ccttgcaaac ctgcttgagc aatttcagca gccggattac cggccctgat
     1141 cttttccgca agccacgttg gctcgttctt cttggtgtca cggagaaatt catatattgc
     1201 gta
//
LOCUS       DASTWE010001122         5413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1355143, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001122 DASTWE010000000
VERSION     DASTWE010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355143"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="gmhB"
                     /locus_tag="VFT30_11705"
     CDS             <1..1024
                     /gene="gmhB"
                     /locus_tag="VFT30_11705"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEU5093344.1"
                     /translation="GAKGEESLGKDIAAQLGGRSVVLSGATSIRELMAVVKRCRLLIT
                     NDTGPMHIASAFGVPVVAVFGPTDWRTTAPYGQERSIVREAVDCAPCLLRECPIDHRC
                     MTRIPVDRVYEAAVEQLAGLSCPSGYSGLAKTDQTDPTNILFGYTIFLDRDGTLNSDP
                     GYINSPDQFELFPGVPEALARLKRAGARLIVVTNQSGVARGFLTRGDLDAVHMKLKRL
                     LDGAGASLDAIYFCPHHPDDGCGCRKPNRGMIDQAVRECSVNLDRSYLIGDHIRDIEL
                     AKRIGARSVLVTTGVVSPQEAERLKTSGPAPNWIASSLAEAADWLLSDVGKVSVQIRG
                     RQVAHP"
     gene            1150..2148
                     /locus_tag="VFT30_11710"
     CDS             1150..2148
                     /locus_tag="VFT30_11710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5093345.1"
                     /translation="MAVSQVNTCSIPCNLCGGIEVSILSNRSRSGKPLQTVICQACGL
                     VWSDPRPHDARQFYEEEYRLSYKNTYSPKSKHVLRAGRVALSRFEKIEQLLSSRKAVL
                     DVGTGGGEFAYLLQSLGHGVSGIEPNRGYADYSICEYGLTVQVGFVQDATFAPDSFDV
                     VTIWHVLEHTEDPGFILALLRSWLKPVGTLVVEVPNVEATCQAPSSTFHEAHLYNFNV
                     VSLRRLAKKHGLYEVKHLISRDGGNITMFFSRMEPLVHDRYEASIPGNCEWISRIVRQ
                     HSNVRRHLTPLPYLRAWQRLCRSLQERREAADERSGKALLDKLYSPQLQLHSVGRS"
     gene            2237..3310
                     /gene="waaF"
                     /locus_tag="VFT30_11715"
     CDS             2237..3310
                     /gene="waaF"
                     /locus_tag="VFT30_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEU5093346.1"
                     /translation="MPSVNPLGSCSSSALKSARILLIKPSSLGDIVHAFPVVSAIKTQ
                     WPGSHITWVVKRQWTDLVERAEGIDRVWPVETTVRSWIREGLALRAQRFDLAIDLQGL
                     FRSGVLARFSGAPMRIGFANAREGSPWFYTQHVPVLSPDIHAVDRYLSVAAALGASLP
                     NKPRFEFRLPEKEMVAVRDLCRCRGFSVDRPWVAMSIGARWPTKRWPLASFAAVVDQL
                     HETHRGPVVMIGGADERTHTNKLRALTDRPFIDLSGDIPLGYLPALLSKATAMVTNDS
                     GPMHIAAALGIPVVAIFGPTSAARTGPYGDGHQVLTSRIPCSPCFSRVCRHDPELECL
                     HLIKPTQAVDVMRPLLMAHVPCR"
     gene            3301..4326
                     /locus_tag="VFT30_11720"
     CDS             3301..4326
                     /locus_tag="VFT30_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5093347.1"
                     /translation="MSMNVLIVRPDGIGDVLLSLPVATQLRRLVPGVTVGFLTSPTVA
                     PLLDRHPDVDYVRTIRFTDPFKELRLAFSQGVEAAIFLKPFRRLMWAAWMAGVPIRVA
                     TRYRWYSLLANRHIYEHRSEFSKHESEYNVEMLKGLGLNPQPISPPVLALTESERAAG
                     ASRWSGLPSPRVVIHPGGVSARRWRLEHYRDLVVTLADRGYGVVLTGSEQERTEFGKG
                     LPVSTALPSGVADHMGNLSLRELMSVIANAHVVVSGATGPAHLAAALGTLTITLFDPR
                     RNNLPVRWKPLGTGVLLRPDVPTCEKCIGEVCPYWDCLDRFTVAKVAAAINKVLENPS
                     PLTVLHT"
     gene            4414..5001
                     /locus_tag="VFT30_11725"
     CDS             4414..5001
                     /locus_tag="VFT30_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU5093348.1"
                     /translation="MNLQGQRDLLLLTEVERDGAVTQRSLASKLGVALGLTNLYLKRL
                     ARKGYIKITTIPSHRVRYLLTPQGFAEKSRLTYLYMEYSLSHYRDMRARLREALSQAA
                     NNGAKRVVIYGTGELAEMAYLSLREMHMTLVGFVNDNQQEAFLSYPVWQPEVLSEWEF
                     DAVLLADIERTVRHREILGQHHVPEVKILALGPSV"
     gene            5055..>5413
                     /locus_tag="VFT30_11730"
     CDS             5055..>5413
                     /locus_tag="VFT30_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEU5093349.1"
                     /translation="MFETNRLHWYALRTKSRHEKFVRDQLNKQGIEPLLPTVKRLSQW
                     KDRKKEIEVPLFSGYCFVRFGKEGKLPVQKVVGVLEVVGSKRPEPIPEEEIESLKTLM
                     TSVLPYDPHPYLHEGMKV"
BASE COUNT         1139 a         1407 c         1604 g         1263 t
ORIGIN      
        1 tggagcgaag ggagaggaat ctctggggaa ggacattgcg gctcagctcg gcgggcgatc
       61 ggttgtctta tccggcgcga cgagcattcg agagctcatg gcggtggtga agcgttgtcg
      121 cttgctgatt acgaacgata cagggccgat gcatattgcg tcggctttcg gtgtgccggt
      181 tgttgcggtg ttcggcccca cggactggcg caccaccgct ccttatgggc aggaacggtc
      241 gattgtgcgg gaagccgtag actgtgcgcc atgcctcctc agggaatgcc cgatcgatca
      301 ccgttgcatg actcggattc ccgttgaccg ggtgtatgaa gcggcagtgg aacaacttgc
      361 gggtctatct tgtccatctg gttactctgg tttagccaaa acagaccaga cagaccctac
      421 caacattctt ttcggctaca ccatctttct cgatcgagac ggaacgctca actccgatcc
      481 cggctacatc aactcccccg atcagtttga gctgtttcca ggagtgcctg aggcgctcgc
      541 caggctgaag cgggccggtg cccgattgat cgtcgttacc aatcaatcgg gggtcgcgcg
      601 aggattcttg acgcgcggtg atctcgatgc cgtgcacatg aaactcaagc gtctactcga
      661 tggtgctggt gcgtcactcg acgcgatcta tttctgtcct caccatcctg atgacggttg
      721 cgggtgccga aaaccgaacc gtgggatgat cgaccaagcg gtgcgagaat gtagtgtgaa
      781 tcttgaccga tcctatctga tcggcgatca catccgcgat atcgagttgg cgaagcggat
      841 tggggcgcga agtgtcttgg tgacgacagg agtcgtttcc ccgcaggagg cagaaagact
      901 gaaaacctcg gggccggctc caaattggat cgcatcctcg ctggccgaag ctgccgactg
      961 gctgttgtcc gatgtaggca aggtgtccgt acagattcgc gggcgtcagg tcgcgcaccc
     1021 gtgagcaggt gatggaggtg gagagactgt gttccgcccg tcttgtgtcg actggggagc
     1081 cggcgtaagt tgtatgggga gaataggacg cgcaaaggaa ccggctatgt tgctcggagg
     1141 gagaggaaga tggcagtgag tcaagtgaac acttgttcca ttccttgtaa tctctgtggt
     1201 ggtatcgagg tgtcgatcct ttcaaacaga agtcgaagcg ggaagccttt acaaacagtc
     1261 atttgtcagg cctgtgggct cgtgtggtcc gacccacgcc ctcatgacgc caggcagttt
     1321 tacgaagagg agtatcgcct ctcctataaa aacacctaca gcccgaaatc gaaacacgtc
     1381 ctgcgcgcgg gccgtgtggc cttatcgcga ttcgaaaaga tcgagcaatt gctgtcgagt
     1441 cggaaagccg ttctggatgt cggcaccggc ggaggagagt tcgcctatct gcttcaatcg
     1501 ttggggcacg gcgtgagcgg cattgaaccg aatagagggt atgccgacta ttccatctgc
     1561 gaatatgggc tcacggttca ggtcgggttc gtgcaagatg cgacgtttgc accggactcg
     1621 ttcgatgtgg tgacgatctg gcatgtactt gagcacactg aagatccggg tttcatttta
     1681 gctctgctcc gatcctggct taagccggtc ggcacgcttg tggtggaagt tccgaatgtg
     1741 gaggcgactt gccaggcccc cagcagcacg ttccatgaag cccacctcta taatttcaat
     1801 gttgtatcat tgcgcaggtt ggccaagaaa cacgggttgt acgaggtcaa gcatctgatc
     1861 tctcgcgatg gtggaaacat cacgatgttc ttttctcgca tggagccgct ggtccacgat
     1921 cgttacgagg cctcgattcc cggcaactgc gaatggatct cgagaatcgt gcgtcagcac
     1981 agcaacgtgc gacgtcattt gacgccgcta ccgtacctac gggcttggca gcgcctctgc
     2041 cgatcacttc aagagaggcg tgaagcggcg gacgagcgga gtggcaaggc actgctcgat
     2101 aaactctatt ctcctcagct gcagctccat tccgtaggac ggtcatgaag aaattcagac
     2161 tgtgcaagga ttaatgacac gcgctctttc ttcgtcttca gtctctggcg aacggggttc
     2221 gctggaaggt tgtccggtgc catctgtcaa tcctctgggt agttgctcct catcggcatt
     2281 gaaaagcgcc cgcatccttt tgatcaagcc cagttctctt ggggacatcg ttcacgcgtt
     2341 tccagtcgtg tcggcgatca aaacacaatg gccgggatcg catattacgt gggtggtgaa
     2401 gcgccagtgg actgaccttg tcgagcgggc ggaaggaatc gaccgtgtct ggcctgtgga
     2461 gacgacggtg aggagttgga tcagagaagg cctggcgctg cgcgcacagc gatttgacct
     2521 cgctatagat ttgcaagggc tgtttcgcag cggcgtcttg gcacgcttca gcggcgcacc
     2581 gatgcgtatc ggattcgcga acgcgaggga agggagccca tggttctata ctcaacacgt
     2641 accggttctc agtcccgata ttcacgccgt cgatcgatat ctttctgttg ccgcggcgtt
     2701 gggagcatct ctgccgaaca aaccgcgatt tgagttcaga ttgcctgaga aggagatggt
     2761 ggccgttcgg gatctctgcc ggtgcagagg cttttcggtg gacaggccat gggtcgccat
     2821 gagcattggg gcgcggtggc cgacaaagcg ctggccgctg gcatctttcg ccgccgtcgt
     2881 ggaccagctg cacgaaacgc atcgtggtcc ggtcgtcatg ataggaggcg cggatgagcg
     2941 cacacacacg aataagttga gagctctgac ggaccgtccg ttcatcgatc tgagcggtga
     3001 tatcccattg ggatacttgc cggcattgct ctcaaaggcg accgctatgg tcacaaacga
     3061 ttccgggcca atgcatatcg ctgccgcgct tgggattccg gtggtcgcga tattcggacc
     3121 cacgagtgcg gcgcgaactg gaccgtatgg agacggccat caggtactga ccagtcggat
     3181 cccttgtagc ccttgtttca gtcgtgtctg ccgacacgat cctgaattgg aatgccttca
     3241 tctcattaag ccgacccaag cggtcgatgt tatgcgaccg ctgttgatgg ctcacgtacc
     3301 atgtcgatga acgttctgat cgttcgccct gacggcatcg gcgatgtcct gctctcgctt
     3361 cccgtggcta cccaactcag gcggcttgtg cccggggtta cagttgggtt tctcaccagt
     3421 cctaccgtcg cgccccttct cgatcgccat cccgacgtgg attatgtgcg aacgattcgc
     3481 ttcacagacc cattcaagga acttcgtctc gccttttcac agggagttga agcggcgatt
     3541 tttctgaagc cgtttcgacg tctgatgtgg gccgcatgga tggccggtgt gccgatccga
     3601 gttgcaacaa ggtaccgttg gtatagcctg ttagccaatc gccatatcta tgaacatcgc
     3661 agcgagttct caaaacatga atcagaatac aacgttgaaa tgttgaaggg cttggggttg
     3721 aaccctcagc ctataagccc gccggtgctc gccctaaccg agtccgaacg cgctgccggt
     3781 gcatcccggt ggtcggggtt accgagtcct cgcgttgtga ttcacccggg aggagtttct
     3841 gcacgccgct ggcggctgga gcattaccgt gatctggtcg tgacattggc ggacagaggg
     3901 tatggtgtcg tgcttacagg cagtgaacag gaacgaacgg agttcggaaa aggtctaccg
     3961 gtgtctaccg cgcttccctc aggggtagcg gatcatatgg gtaatctatc gctgcgagag
     4021 ctgatgtcgg tgatcgccaa tgctcatgtc gttgtctcag gagccacagg gcccgctcac
     4081 ctggcggcag ctttgggtac tctcacgatc accttgtttg accctcgtcg aaacaatctg
     4141 ccggtgaggt ggaaaccgct tgggacggga gttttgctgc ggccagatgt tcccacttgc
     4201 gaaaaatgta tcggagaggt ctgcccctac tgggattgtc ttgatcgatt cactgttgca
     4261 aaggtggctg ccgctatcaa taaggtcctg gaaaaccctt ctccactcac tgtccttcat
     4321 acataagggc agaactcaaa gataacgctc cggattagcc cttgactcca agtcgcgtct
     4381 gtgttcatat tctgaacggc aggttctaac tatatgaatc ttcaagggca acgagatctc
     4441 ctcctgctaa ctgaggttga acgagatggt gcggtcactc agcggtccct cgcttcaaag
     4501 ctaggggttg ctctgggctt gaccaatctc tatcttaagc ggttggcacg gaagggctat
     4561 atcaagataa ccacgattcc ctcacatcga gttcgatatt tactgactcc gcaagggttt
     4621 gccgagaaat ccaggcttac ctacctatat atggagtatt cgctatctca ctaccgcgat
     4681 atgcgtgcgc ggctacgaga agcgctgtcc caggcggcca ataacggagc gaaacgagtc
     4741 gtcatatatg gaaccggtga actggcggag atggcatatc tatcgctccg agaaatgcat
     4801 atgaccttgg ttggctttgt gaatgataac cagcaagagg cgttcttatc ctatccggtc
     4861 tggcagccgg aggtgctgag tgaatgggag tttgacgcgg tgctgctcgc ggatattgaa
     4921 cgaacggtac ggcaccgcga aatattgggc cagcatcacg ttccagaagt caaaattcta
     4981 gcgctgggcc cgtcggtcta agcagttgaa ggatagtcga tttttccgtt cactgtaagt
     5041 gagagggcga agctgtgttc gagactaacc gtcttcactg gtatgcgctt cgtacgaaat
     5101 ctcgccatga aaaattcgtg cgggatcagc ttaacaaaca gggcatcgaa ccattgttgc
     5161 cgacggtgaa gcggctcagt caatggaagg atcgcaaaaa ggaaatcgaa gtgccgttat
     5221 tttccggcta ctgctttgtg cgattcggaa aagaggggaa gctgccggtg cagaaggtgg
     5281 ttggagttct cgaagttgtc ggcagcaagc gaccagagcc cattcccgag gaggaaatcg
     5341 aatcgctgaa gaccttgatg accagtgttc tcccctatga tccacatccg tacctgcatg
     5401 aaggcatgaa ggt
//
LOCUS       DASTWE010001123         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_223828, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001123 DASTWE010000000
VERSION     DASTWE010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223828"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFT30_11735"
     CDS             complement(<1..262)
                     /locus_tag="VFT30_11735"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEU5093350.1"
                     /translation="MTPHTSRIPRWFLLLTALVTGAVVMALEILGSRLLAPVFGSSLF
                     VWGALIGVILAAMSSGYAFGGWVSDRYTGGGVLAALLLFSGSW"
     gene            449..1762
                     /locus_tag="VFT30_11740"
     CDS             449..1762
                     /locus_tag="VFT30_11740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU5093351.1"
                     /translation="MHISVIGSGYVGLVTGACFAEFGVHVTCMDSDNRRIEKLEKGEI
                     PFFEPGIAELVAKGVKEGRLSFTTDIAQAVDKALVIFIAVGTPPSPDGSADLSFVKEV
                     GRGIARHMSSYKVIVTKSTVPVGTGEAIREVVKKTQKDPIRFDMVSNPEFLREGSAIE
                     DFMRPNRVVIGADSDQAIAIMKDLYRPLYLIETPIVVTDVPTAELIKYASNAFLATKI
                     SFINEIANLCEKVGANVQMVAKGMGLDHRIGAKFLHAGPGFGGSCFPKDLAALIQTGE
                     RHGYPMQIASAASRVNDSQRGRMVDKIREALGGLKGKTLAMLGLSFKPNTNDLREAPA
                     LTIGRVLLAEGASIRAYDPEALAEACQMMPELQACRDTYHAAEGADALVIMTEWNVFR
                     NLDFEKLKSVMRVPVLLDLRNIYDPERIAAAGFKHVSVGRMAQSP"
BASE COUNT          489 a          554 c          570 g          446 t
ORIGIN      
        1 tccagcttcc cgaaaagagc aacagggcag ccagcacccc tccgccggta taacgatcgg
       61 agacccaacc gccgaacgcg tacccgctac tcatggcggc caagatcacc cctatcaatg
      121 cgccccagac aaacagcgaa ctgccgaaca ccggtgccaa caaccggctg ccgagtattt
      181 ccaatgccat caccaccgca ccggtcacaa gggcggtcag gagcaaaaac cagcgtggaa
      241 ttcgtgaagt atgaggagtc attgattgaa cggaagggat gggaaactcc ttgtcacgcg
      301 aaacagcgtc ggattgtact caacgcattt tccgagagtc aacgcaaccg ggtggactct
      361 atgacgctga atgatcctgg aattcgttgc tggataggat tcaggttgct atactttccg
      421 acaattacct atgcgatgag gagggtttat gcacatcagt gtgataggaa gcggctacgt
      481 cggactcgtt actggagcat gcttcgccga gttcggcgtc catgtcacct gtatggacag
      541 tgataaccga agaatcgaaa agctcgagaa gggggagatc ccgttttttg aaccaggaat
      601 tgcggaactt gttgccaagg gagtcaaaga aggcagactg agtttcacta cagacatcgc
      661 tcaggcggtc gacaaggcgt tggtaatttt cattgccgtg ggaacccctc caagtcccga
      721 cgggagtgcc gacttatcgt tcgtaaaaga ggtggggaga ggcatcgccc gccatatgag
      781 tagctataag gttattgtga ccaaatcgac cgtgcccgtt gggacaggag aagccattcg
      841 cgaggttgtc aagaagactc agaaagatcc tattcggttc gatatggttt cgaacccgga
      901 gtttctccga gaagggtcgg ccattgagga cttcatgcgg ccgaaccgcg tcgtaatcgg
      961 ggcggacagc gatcaggcga tcgccatcat gaaggatctc tatcgtcccc tctatctgat
     1021 cgaaacaccg atcgtcgtga cggacgttcc gactgccgag ctcatcaagt atgcatccaa
     1081 cgcgttcctg gcgacgaaga tctcctttat caacgagatt gccaatcttt gcgaaaaagt
     1141 cggagccaat gttcaaatgg tggccaaggg catgggactg gatcaccgaa tcggggcgaa
     1201 attcctccat gcggggccgg gttttggagg atcatgtttt cccaaggatc ttgccgcact
     1261 catccagacc ggcgagcgcc atggctatcc catgcagatt gcctccgccg cttcacgagt
     1321 gaacgacagt cagcgaggac ggatggtcga caagatccgt gaggcgttgg ggggattgaa
     1381 aggaaaaaca ttggcgatgc tcggcctctc cttcaagccg aacaccaacg atctcagaga
     1441 agccccggcc ttgacgatcg gtcgagtcct cctggcagag ggcgcgagca tccgcgcgta
     1501 cgatccggag gctctggcag aagcctgcca aatgatgcct gaactccaag cctgccgaga
     1561 cacctaccat gcagccgaag gggccgatgc tctggtgatt atgaccgaat ggaatgtgtt
     1621 tcgaaacctg gattttgaaa agttgaagtc tgtcatgcgg gttcccgttt tgcttgatct
     1681 ccgaaacatt tatgatcctg aacgcattgc ggctgccggc ttcaaacatg tatccgtggg
     1741 gcgtatggca cagagcccct agcaggctgt tgaaaaagtc ggccagctcc gttcacaaac
     1801 gagccatgcc ttattcggcg cggcgtgaac ctcgctcccg tccgcaaacg taaggcctta
     1861 ttcgtcgcct cgcggccttg ctgaacgacc tttttgacca gcctgcaaac tcatgtgaaa
     1921 tgattgcgtc aatatcctgc tacgtgggtt aggtcagcct cggcagctcg gccttagcct
     1981 gctccttggt tttcggcttg taccccattc gatcgagcac tttcatgatt cgggtcttta
     2041 atcgatcatc agcgtcggt
//
LOCUS       DASTWE010001124         4856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1613817, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001124 DASTWE010000000
VERSION     DASTWE010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613817"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFT30_11745"
     CDS             <1..487
                     /locus_tag="VFT30_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEU5093352.1"
                     /translation="KADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFLLS
                     AEYVMSRGNQNVMLCERGIRTFETQYRNTLDLAAIPTLKELSHLPVIVDPSHATGKWD
                     LVAPMSKAAVAAGADGLLIEVHSNPECALCDGEESIKPSKFKALMSDLRNIAKAVGRE
                     L"
     gene            495..1388
                     /locus_tag="VFT30_11750"
     CDS             495..1388
                     /locus_tag="VFT30_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU5093353.1"
                     /translation="MASHFKHVAIIGVGLIGGSLGMILRRKALADHVVGVGRRVENLK
                     TAVALGAIDRYVADPQEGVRGADLVILATPVDTYERHLHEWAHCLTPGAIVSDVGSVK
                     GTLVERSEATMPVGVHFVGAHPIAGKEKTGVAAGSDQLFKGARCILTPTKRTDTTALD
                     RIRQLWEETGSIVLTMDPHLHDQIMGAVSHLPHVAAFALINALAELRDQQVPSLDLAG
                     HSGGGLRDTTRIAASSPEMWRDIFLWNRDNVVSYIDRYARSLEELKQLIKAGDTAGIE
                     KLLERAKGEREKLNSFSPSNS"
     gene            1385..2707
                     /gene="aroA"
                     /locus_tag="VFT30_11755"
     CDS             1385..2707
                     /gene="aroA"
                     /locus_tag="VFT30_11755"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU5093354.1"
                     /translation="MTSLTIIPGRPLKGTITVPGDKSLTHRAIILTALAEGTSTISGY
                     CPGEDCLNTMRAFQGLGIPITQTATELTVHGKGFWGLTEPSAPIDCGNSGTGIRLLTG
                     LLSGQDFFSVLTGDESIRRRPMGRVVKPLREMGAVIGGRKGGELAPLAITGAGLHGIA
                     YTSPVASAQMKSSLLLAGLFAQGKTRYKEPSLSRDHTERMFQFFGIALAKEEGALVLQ
                     GRPSVGWRGVHMTIPGDFSAAAFFIVGATIVQGSDITIRNVGMNPTRTGLLEVMRKMG
                     ADIQVLDQREEAGEPVGDLRVKSSTLKGITIGHDLIPKTIDEFPVLCVAAAVAEGDTV
                     ISGAEELRVKESDRIATMSRELKAMGALIEERPDGMIIRGLGRGGENGRLKAAGKTES
                     HGDHRVAMSLAIGGLTAEESMTIADTSCMDTSFPNFEKALVDLLAEPA"
     gene            2750..3409
                     /gene="cmk"
                     /locus_tag="VFT30_11760"
     CDS             2750..3409
                     /gene="cmk"
                     /locus_tag="VFT30_11760"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU5093355.1"
                     /translation="MIIAIDGPAGVGKSTVAKLLAARLGYLYLDTGALYRAIAWKTLQ
                     SRIRPTDHDQVTTLLPITSIHMRFHQCAMQVLVDGIDVTAELRTPEVTEAASVVSAIP
                     AVREWLLPIQRQIGQRGSVVAEGRDIGTKVFPAAPFKFFLEADSDVRVARRHRELVAA
                     GRGGTVETTSRDLSDRDRRDRTRSVAPLIPAGDARIIDTSALSPDQVVEQMIAAVSTE
                     S"
     gene            3406..4098
                     /locus_tag="VFT30_11765"
     CDS             3406..4098
                     /locus_tag="VFT30_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5093356.1"
                     /translation="MSGIFYGFLWALARVVAWICFRYRVEGEIPRTGGLLIAANHASY
                     FDIPLLGCGMRRRAWYLGRNDLFPIPVLNGILRALGWIPVRLGRLDREAFGKAINLIR
                     AGHVVVIFPEGGRSYDGHLRPPKAGIGVIVSQTGCPVVPAYLKGTFDVLPAGARWPRL
                     RQVTVRFGAPIQFETGEQKERAETKQFYQQVSRTVIEHIAALGQVPVPKGRVDLAPET
                     PNRTTVDAHNAE"
     gene            4154..>4856
                     /locus_tag="VFT30_11770"
     CDS             4154..>4856
                     /locus_tag="VFT30_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5093357.1"
                     /translation="MGTVSNSSDTQLDRNALAALYEETFRNLEEGTITEGRVVALTKD
                     KVIVDIGYKSEGMIPSDQFSSEELHNLKIGDRLQVYIEECEDADGNLVLSKEKADKMK
                     IWEELETLYKEEKSIEGKIVSRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGKTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDKKRQTTLANLKEGQLIQGTVKNITDYGSFID
                     LGGIDGLLHITDMSWG"
BASE COUNT         1122 a         1226 c         1476 g         1032 t
ORIGIN      
        1 gaaggcggat atcattcaga tcggcgctcg gaacatgcaa aacttcgagc tcctcaagga
       61 ggtcggcgcg tacgacaagc cggtccttct gaaacgaggt ctgtcggcaa cgatcaaaga
      121 gtttctcttg tcggcagagt acgtcatgtc acgcggcaac cagaacgtca tgctgtgcga
      181 gcggggtatt cgcacgttcg aaacgcaata tcgcaatacc cttgatctcg cggccattcc
      241 tacattgaaa gagctttcgc atctccccgt catcgtcgac cccagccatg ccaccgggaa
      301 gtgggatctc gtcgctccga tgtcgaaagc ggctgtggcg gcaggggcgg acggcctgct
      361 catcgaagtc cattcgaacc ccgagtgcgc cctgtgtgac ggtgaagagt ccattaaacc
      421 ttctaagttc aaagcgctga tgagtgacct caggaacatt gcgaaggcag ttgggcgaga
      481 actatagaga cgctatggcg agccatttca agcacgtcgc gattattgga gtggggctga
      541 tcggcggatc actcggtatg atccttcggc gaaaagcgtt ggccgatcat gtggtcggcg
      601 tcggccgtcg tgttgagaat ctcaagacgg cggttgcttt gggtgcgatc gatcgatacg
      661 tggccgatcc tcaagaaggc gtgcgtggag cggatttggt gatccttgca acacctgtcg
      721 atacctacga gcgacacctc catgaatggg cccattgtct gactccaggg gcgatcgtca
      781 gcgatgtagg cagcgtgaaa ggaaccttgg tcgaacgatc ggaagcgact atgccggtag
      841 gcgtacactt tgtgggagca catcccatcg cggggaagga aaagacggga gtcgcggccg
      901 gatccgatca gttgttcaag ggcgcacgct gtattctgac gccgacaaaa cgaacggata
      961 cgacggcact tgatcggatt agacagctgt gggaagagac cggctcgatt gtcttaacga
     1021 tggatccgca tctgcacgat cagatcatgg gagcagtcag ccatctgccc cacgtggccg
     1081 cctttgcgct tatcaatgcc ttggccgagc ttcgcgatca gcaggtcccc tccttggatc
     1141 ttgccggcca ctctggggga ggattacggg atacgaccag gatcgccgcg agttctccgg
     1201 aaatgtggcg agacattttc ttgtggaatc gggataatgt ggtgtcctac atcgacagat
     1261 atgcacggtc tctggaagaa ttgaagcagt tgattaaggc tggagataca gcgggaatcg
     1321 agaagttgct tgagcgggcc aaaggcgagc gtgaaaagtt gaatagtttt tctccgagca
     1381 actcatgaca tcattgacga tcatcccggg ccggccactc aagggaacga tcacagttcc
     1441 cggcgacaag tctctcaccc atcgggctat tatcctcacg gcactggcag aaggaacgag
     1501 cacgatatcg ggttattgtc caggagaaga ttgtctgaac acgatgaggg cgtttcaggg
     1561 gctcggaatt cctattacac agactgcaac cgaattgact gtccacggaa agggtttttg
     1621 ggggttaacc gaaccgagtg ccccgatcga ttgcggcaac tcgggaaccg gtattcgtct
     1681 gctcacgggt ctattgtccg gacaagattt tttctcagtt ctcacaggcg acgaatcgat
     1741 cagacggcgt cctatggggc gggtcgtcaa gccactgcgc gagatgggcg cggttattgg
     1801 aggccgtaaa ggcggagaac tggctccctt ggcgattaca ggagccggcc ttcacggtat
     1861 cgcgtacacc tcgcccgtag ccagcgcgca gatgaagtcg tcgctcctgc ttgccggcct
     1921 ctttgctcaa gggaagacac gttataagga accaagcctg tcacgagacc atactgagcg
     1981 gatgtttcag ttctttggaa ttgctctcgc aaaagaagaa ggtgccctgg tcttacaagg
     2041 gcgaccttcg gtcggatggc gaggcgttca tatgaccatt cccggtgatt tctcggccgc
     2101 cgcgttcttc atcgtcggag caacgatcgt gcagggatcc gacatcacca ttcgcaatgt
     2161 cgggatgaat ccgaccagaa ccggcctact tgaggtcatg agaaagatgg gagccgatat
     2221 tcaggttttg gatcagcgag aagaggccgg tgaaccggtc ggggatctgc gtgtgaagtc
     2281 atccacactg aagggcataa cgatcggcca cgacctcatt ccgaaaacaa tcgatgaatt
     2341 tcccgtgctg tgcgtggccg ccgctgtggc ggagggggac actgtgatct ccggagcaga
     2401 ggaactacga gtcaaagaga gcgatcgcat tgcgactatg agccgtgagt tgaaggccat
     2461 gggagctctc atcgaagaac ggccggacgg gatgatcatc cgaggattag gcaggggggg
     2521 agagaacggc cggctgaaag ccgcaggcaa gaccgaaagt cacggagatc atcgtgtagc
     2581 catgtctctc gctatcggtg ggcttacggc ggaagagagt atgacaattg ccgacacgag
     2641 ttgcatggat acgtcgtttc caaattttga gaaggcactc gtcgatctgt tggctgagcc
     2701 tgcctgaaag ccgtgtcaga tgagacaggt cgaaactgag aggcgttgag tgattattgc
     2761 gattgatgga ccagccggag tcggcaagag cacagtagca aaattattag ccgctcgtct
     2821 tggttacctc tatcttgata cgggggcact gtaccgagct atcgcatgga agaccttgca
     2881 atcaaggata cgtccgaccg atcatgatca agtgacaaca ttattgccga ttacctcgat
     2941 tcacatgcgg tttcaccagt gcgcgatgca ggtcttggtc gatggcatcg acgtcacggc
     3001 ggaacttcgt acgcctgaag ttaccgaagc ggcctccgtc gtgtccgcta tcccggctgt
     3061 ccgtgagtgg ctgctgccga tccaacgtca aatcggccag agaggctcgg tcgttgcaga
     3121 gggacgggat atcggcacca aggtttttcc cgcggctccg ttcaaattct ttttggaggc
     3181 ggactcagat gtacgagttg ccagacgaca ccgtgaactg gtcgccgcgg gtcgtggtgg
     3241 gacggttgaa acgacgtccc gggatctgtc ggaccgagat cggcgggatc ggacccgttc
     3301 ggtcgcccca ttgattccag cgggtgacgc acgaataatt gacacgtctg ctctcagccc
     3361 tgaccaagtg gtggagcaga tgattgcggc agtgtcgacc gagtcgtgag cgggattttc
     3421 tacggattct tgtgggccct ggcacgagtc gtggcctgga tttgctttcg atatcgtgtt
     3481 gaaggggaga ttcctcggac cggaggcctg ctgatcgcgg caaaccatgc cagctacttc
     3541 gatattccgc tactcggctg cggaatgcgt cgaagggcct ggtacttggg acggaatgac
     3601 ttatttccga tcccggtatt gaacggtatt ttgcgggcgt tgggctggat cccggtcagg
     3661 ttgggacgcc tcgaccgaga ggcatttggg aaggcgatca acctgattcg agcaggtcac
     3721 gtggtggtca tttttccaga aggcggacgc agctatgacg gtcacctccg gccaccgaaa
     3781 gcgggtattg gagtgatcgt gtcgcagact gggtgtccgg tcgttccagc gtatctcaag
     3841 gggaccttcg acgtactccc cgcgggggct cgctggcccc gattgcggca ggtcacggta
     3901 cgattcgggg cccctattca gttcgaaacg ggggaacaaa aagagagggc ggaaacgaaa
     3961 cagttctatc aacaggtcag ccgtacggtg atcgagcaca ttgcagcctt aggtcaagtt
     4021 cccgttccaa aggggagagt tgacctggcc cccgaaacac cgaacaggac gaccgtcgac
     4081 gctcacaatg ctgagtgagc ccggcgactt caccatcagc acccgacgag agtcggaaga
     4141 gtaggacgtt catatgggta cggtatccaa cagcagtgac actcagttag accggaatgc
     4201 attggcggca ttgtatgaag aaacctttcg taacctggaa gaaggcacga ttacagaagg
     4261 ccgggtggtg gctttgacca aggacaaggt catcgttgat atcggttaca aatcagaggg
     4321 catgatcccg agcgatcagt tctcttccga ggaacttcac aacctcaaga tcggtgaccg
     4381 gctccaagtc tatatcgaag aatgtgaaga tgcggacggc aatctcgttc tttccaagga
     4441 aaaagcggac aagatgaaga tttgggaaga actcgaaacg ctctacaaag aagagaagag
     4501 tatcgaaggc aagatcgtct cacgcatcaa gggcggcatg atggtcgata tcggagtcaa
     4561 agcgttcttg cccggctcgc agattgatct tcatccggtc cgtgatctgg atgggctcgt
     4621 tggaaagact tttcccctca agatcatcaa gatcaaccac aggcgaggca atgtggtcgt
     4681 gtcacgccgc gtgttgttgg aagaaacacg ggataaaaag cggcagacga cgctggccaa
     4741 tctcaaagaa ggccagctca tccagggtac cgtcaagaac atcaccgatt acgggtcgtt
     4801 tatcgacctc ggcggtatcg atgggttgct gcacattacc gacatgtcct ggggtc
//
LOCUS       DASTWE010001125         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1064562, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001125 DASTWE010000000
VERSION     DASTWE010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064562"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFT30_11775"
     CDS             <1..911
                     /locus_tag="VFT30_11775"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU5093358.1"
                     /translation="GLVAFPTETVYGLGCDAMNADAAAKVFEAKQRPQFDPLIVHIAD
                     PKQLNVVIESLPVLGQRLMDAFWPGPLTLVLPKQPAVPDLVTAGLSTVAVRMPNHPVA
                     QAFIREAGTPIAGPSANPFGYVSPTTAQHVADGLEGKVDLILDGGPCPVGVESTIVSL
                     AGPQPELLRPGSITIEQLSAVIGSIRRLSAVNEKPIAPGQLSRHYATQTHLTILAAGG
                     ARPILKDDERAGLLIHSQARDTDDRFAAVEVLSPTGDLKEVARRLFAALRRLDSLGLD
                     RIYVEPCKEEGLGVAIMDRLRRCAAP"
BASE COUNT          250 a          332 c          309 g          251 t
ORIGIN      
        1 gaggactcgt tgcgtttcca accgaaacgg tctatggact cggttgcgat gcgatgaatg
       61 ccgatgcggc agcgaaggtc ttcgaagcca agcaacgccc tcagttcgac ccgctcatcg
      121 tccacatcgc tgatccaaag cagttgaatg ttgtgattga atccctgccc gttctgggtc
      181 agcggctcat ggatgccttc tggcccggtc cactgacttt ggtattgcca aaacagccgg
      241 ccgttccgga tctcgtcact gccgggcttt caacggtagc ggtcagaatg ccgaaccatc
      301 cggtggcaca ggcgtttatt cgagaagcag gtaccccgat tgccggcccc agcgccaatc
      361 cctttggcta tgtcagcccg acgaccgctc agcacgttgc tgatgggctt gagggcaaag
      421 tagatctcat actcgacggc ggaccatgcc cggtcggggt cgaatcgacg attgtttcac
      481 tggcaggacc acaaccagaa ttgttacgtc ctggcagcat caccatcgaa caactcagcg
      541 ccgtcatcgg ctctattcga cggttgtcgg ctgtgaatga aaagccgatc gcacccggtc
      601 aattgtcacg ccactacgcg acacagacgc acctgacgat cttagctgca ggtggagcaa
      661 ggcctatctt gaaagatgac gagcgcgcgg gcttactgat ccactcgcaa gcaagagata
      721 ccgatgatcg ctttgcagcc gttgaagttc tatctcctac aggcgatcta aaggaagttg
      781 cacgccgcct gtttgcagca ttgcggaggt tggactccct tggtctggat aggatctatg
      841 tggagccttg caaggaagaa ggacttggag tcgcgatcat ggaccgcctg cgccgctgcg
      901 ccgcgccatg acggcctctt gttcttccta acgcacagaa cgagcactcc ccactcttct
      961 tctttgctta tcactgccac tttccactca tcgtcacgtc cgctggaaca gcagcttggc
     1021 aatggagaca cggttcttat cgataatcgc gaccaggatg atcacgtagg agagaaatcg
     1081 tatgagatac acaagcggct cgaattcgtc attcccgagg tgtacggcca atgccagacg
     1141 gc
//
LOCUS       DASTWE010001126         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_94760, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001126 DASTWE010000000
VERSION     DASTWE010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94760"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFT30_11780"
     CDS             complement(<1..296)
                     /locus_tag="VFT30_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5093359.1"
                     /translation="MVRVILPQHLRTLARVNGEVTLEVSGPVTQRAVLDALEARYPML
                     RGTVRDHVTHKRRPFVRFFACEQDLSHESPDTPLPDAIVTGSEPFLIVGAMAGG"
     gene            complement(429..1610)
                     /locus_tag="VFT30_11785"
     CDS             complement(429..1610)
                     /locus_tag="VFT30_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU5093360.1"
                     /translation="MSSVRVLVGTRKGAFILTSDGARKRWDVQGPHFGGWELYHLKGS
                     PVDPNRLYASQTSSWFGQVIQRSDDGGKTWSAPGTKPEDLMEPDGMPKGESNMFLYDT
                     SAETGKPLTTHQHYDGSQRPWEFKRVWHLEPSLTDPDTVYAGVEDAALFRSSDGGRTW
                     HELAGLRDVKGSLWQPGAGGMCLHTIILDRTNPQRIYIAISAAGAFRTDDGGKTWRAI
                     NRGLKSQYELPDADADVGHCVHNIAMHPSRPNVLFMQKHWDVLRSDNAGESWHEISGN
                     LPSDFGFPIVVHAHEPNTIYVVPIKSDSEHYPPEGKLRVYRSRSGGNEWEALTKGLPQ
                     ENCYVNILRDAMTVDHLDPCGIYFGTTGGQVYGSTNGGDSWEPIVRDLPAVLSVEVQT
                     L"
     gene            complement(1803..2402)
                     /locus_tag="VFT30_11790"
     CDS             complement(1803..2402)
                     /locus_tag="VFT30_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEU5093361.1"
                     /translation="MQLRPLAVTTLCIMLTVAPVFAKEKKHEKAMDPQAMMEVWKKLA
                     QPGEPHKLFAGLAGSWTTQTKEWMEPGKPPMESTGTSEMKMLLDGRFLFQEYDGQMMG
                     QPFSGVGIDGYDNLTKKYVTAWIDTMGTGIFFMEGTASADGKTITLRGSHPMPGGGKM
                     SHRAVWKIADVNNPTFEMYGAHGKEKEMKFLEIVYTRKQ"
BASE COUNT          516 a          749 c          672 g          652 t
ORIGIN      
        1 tacccccccg ccatggcccc tacaatgagg aacggctcgc ttcccgttac aatggcgtct
       61 ggaaggggag tatccggcga ctcatgagag aggtcctgct cgcaggcgaa aaaccgcacg
      121 aacggccggc gcttgtgtgt gacgtgatca cggactgttc cgcgtaacat cggataacgg
      181 gcttcaagcg catcgaggac cgcacgttgc gtcaccggac cgctgacctc aagggttact
      241 tcaccgttga cacgcgccag ggtacgcaaa tgttgcggca gaatcacgcg caccattatt
      301 cacctattcg gccaggagag ctgatggcgt attgtagggc acaagcacaa agagaagaat
      361 attatggtgc atggctttta cgctttctct ttgttcctac cataagccat cagctatgca
      421 ttttgtcgtc acaacgtttg gacctcgacc gacaacaccg ctggcaggtc ccttacaatc
      481 ggctcccaac tgtccccccc gttagttgat ccatagacct gtcctccggt cgttccgaaa
      541 tagattccgc agggatcgag atggtcgacg gtcatcgcgt cccgcagaat attgacgtaa
      601 caattttctt gcggcagacc cttcgtcagc gcctcccatt cgtttcctcc ggatcggctg
      661 cgatacacgc gcagctttcc ttccggtgga tagtgttcgg agtcgctctt gatcggcacg
      721 acatagatcg tgttcggttc gtgagcgtgt actacgattg gaaatccgaa atcgctcggc
      781 aagttgccgc tgatttcatg ccaggactcg ccggcattgt cgctcctcaa cacgtcccag
      841 tgcttctgca tgaacagcac gttcgggcgt gatggatgca tggcgatatt gtgcacacag
      901 tggccgacat ctgcgtccgc atccggcaac tcatattggg atttcaagcc gcggttgatc
      961 gcccgccatg tcttgccacc gtcgtcggtt cggaacgcgc cggctgcgga gatggcaata
     1021 tagatgcgct gaggatttgt ccggtccagg atgatcgtat gcaaacacat ccctccagcc
     1081 ccaggctgcc acagacttcc cttcacatcg cgtagcccgg caagttcgtg ccaggtacgc
     1141 ccaccgtcgg acgaacggaa caaagcagcg tcttcgacgc cggcgtacac cgtatcagga
     1201 tcagtcaatg acggttcgag atgccatact cgtttgaatt cccacggacg ctgcgagccg
     1261 tcataatgtt ggtgcgtcgt gagcggtttt cctgtttcag cggaggtatc gtagagaaac
     1321 atgttgctct cgcccttcgg catgccgtca ggttccatga gatcttccgg tttggttccg
     1381 ggcgcgctcc atgttttccc gccgtcatcc gagcgttgaa tgacttgtcc gaaccagctg
     1441 ctggtttgcg aggcatatag cctgttcgga tcaaccggtg agcccttgag gtggtacagc
     1501 tcccatcctc cgaagtgcgg cccttgcacg tcccaccgtt tgcgagcgcc gtccgatgtg
     1561 agaatgaatg cacccttgcg ggtgccgacc agtactcgaa cactgctcat tgcatagctc
     1621 ctttctttcg atgaccggtt tttatggtca gtccacagtt tctcgtaagc tagtcgtccg
     1681 accgcaagaa aatcgacagc ggtgtgaaat gtgctacagg attcacgata tgagcgaaag
     1741 acacaaggct gctcttttat cctggtgcca tacgctattc gcgtatttgc cctaaatacc
     1801 gattactgtt ttctggtgta gacgatctcc aagaacttca tttccttttc cttaccgtgg
     1861 gcgccgtaca tctcgaacgt agggttgttg acgtcggcga tcttccagac tgcgcgatga
     1921 gacatcttcc cgccgccagg catgggatgt gagcctcgca gtgtgatggt cttcccgtcg
     1981 gcgcttgccg tgccttccat gaagaaaata ccggtcccca tcgtatcgat ccaggccgtg
     2041 acatatttct tggtcagatt gtcataccca tcgatgccga ctcccgagaa gggctgcccc
     2101 atcatctgac cgtcgtactc ttggaagaga aaccgtccgt cgagcaacat cttcatctcc
     2161 gaggtgccgg tggattccat cggcggcttg cccggctcca tccattcttt tgtttgcgtc
     2221 gtccaactgc ctgcgagacc ggcgaatagt ttgtgcggct caccgggctg ggccagtttc
     2281 ttccagactt ccatcattgc ttgagggtcc attgccttct catgtttctt ctctttcgcg
     2341 aaaaccggtg cgacggtcag catgatgcac aatgtggtta ctgccaaggg tctcaattgc
     2401 atggtggcct ccttcttgtt gttatgaatg aatgtgtcat tcgatgttac cgatagccgg
     2461 ttgcatcggt cgatttccca gtataacgct ggttcatgaa gcagctaggc actgaccagg
     2521 attttaaatc caccatatgc catgcgcttc acatcgaacg gcatcgactt ttcgtcgccc
     2581 atttctcca
//
LOCUS       DASTWE010001127         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_223984, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001127 DASTWE010000000
VERSION     DASTWE010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223984"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..526)
                     /locus_tag="VFT30_11795"
     CDS             complement(26..526)
                     /locus_tag="VFT30_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEU5093362.1"
                     /translation="MKNMPTNKSIISLAIGSAFAVTLGTAPIASANESPFTVQSLGKG
                     YMVAESHDYGEKKEGYGEKKEGYGGKKDREGRCGMSMADTDGDGRVSRDEHARHAEKM
                     FDMMDKNKDGFIDKDEAAAMRKKHAQGHGQSRPYGSEGQGPGYGEAYGTRSGDRQLQH
                     MKPMGE"
     gene            820..>1025
                     /locus_tag="VFT30_11800"
     CDS             820..>1025
                     /locus_tag="VFT30_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093363.1"
                     /translation="MNDWNDLSDRAEALLTRIEGLLHPVQPEPGLNAALAFRWRRYGN
                     KGFIEAVAHPHHITFDALHGIERQT"
BASE COUNT          212 a          293 c          241 g          279 t
ORIGIN      
        1 ccacagtggc ggctgaaccc cattcttact cccccatggg cttcatatgt tgcagctggc
       61 ggtcccccga ccgtgttcca tatgcttcac cgtatccggg cccttgccct tcggatccat
      121 aaggccgaga ttgaccatgg ccttgagcgt gcttcttccg catcgcggcg gcttcatctt
      181 tgtcgatgaa accgtccttg tttttgtcca tcatatcaaa cattttttcg gcatggcgcg
      241 cgtgttcgtc cctgctgacc cggccatcgc cgtcggtatc ggccatggac attccacatc
      301 ggccctcgcg atctttcttc ccgccatacc cctctttttt ctctccgtat ccctccttct
      361 tttcaccgta gtcgtgggat tccgcaacca tatagccttt acccagcgat tgcacggtga
      421 aagggctctc attggcagat gcgatcggtg cagtaccgag cgtcacagca aaagccgagc
      481 cgatggcgag agaaataatt gatttgttcg tgggcatatt cttcatgatg ttttctccaa
      541 aatttgttga aacttgaatg aagttgaaag gcgggtgctt tctgatcgcg ctgacgcaat
      601 atagatatag accatttcat ttctttgctt ctttttggca atttttgtta cgatggagcc
      661 gaatcatggt atttcagcct tgcaataaac aatcttgacc gctttagctg agcggttcag
      721 tacgatagca cacatagcag gaggaaccga caaccactga tgtcgcccct ttccgcaacc
      781 cgcagcaaag acgtacgttc tatagacaaa cccctgctca tgaatgattg gaatgacctg
      841 tccgaccgcg cggaggccct gcttacccga attgaagggt tgctacatcc tgtccagccg
      901 gagcctggct tgaatgccgc gctggcattt cgctggcgta gatacggtaa taaggggttc
      961 atcgaggcgg tcgcccatcc tcatcacatc accttcgatg ctttacatgg catcgagcga
     1021 cagac
//
LOCUS       DASTWE010001128         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_838231, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001128 DASTWE010000000
VERSION     DASTWE010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838231"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..>1102)
                     /locus_tag="VFT30_11805"
     CDS             complement(85..>1102)
                     /locus_tag="VFT30_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU5093364.1"
                     /translation="TGDFTLFNGQHEVGIGEVQVAPAMLRVGDQANIRFIAKNNGVSD
                     ETFDVTVRTLGPGGQSLSTSIAHVTDLPSGAERVVPTATEPNITWDTRNLPWGTYIVQ
                     ASITTPIAGDPQSSNDSNSTFVELAPCDSDGDGVNDKIDNCPTVSNPDQFNCDGNEPG
                     GELGDACDTPRLRSFVPSCNIPGGATVTVIGSGLGRITASDITLGTVKAAAITRQNAC
                     QVEFTNPTANNFPTGSLTIATMPPLQMPVCCAMPTISAIAPSQGRPTEEITIIGCGFS
                     GVIVSLVLQPGSSPIQVGPPATDDQKITFRIPNIVPGFYRIALERPGMQPIISSTTLR
                     VDPP"
BASE COUNT          242 a          301 c          309 g          250 t
ORIGIN      
        1 cgactggaat cgatccagcg cggccggtca agatcgaagg acgggccccc ggtagtgaaa
       61 acacgcaatt ctgatttccg cttcctacgg aggatcgacc ctgagggttg tcgaactgat
      121 aattggctgc attcctggac gttccaacgc gatcctatag aagcccggca cgatgtttgg
      181 gatcctgaag gttattttct gatcgtccgt agcaggggga ccaacctgta tcggcgacga
      241 ccctggctgt aagacaaggg acacgatcac ccctgaaaat ccgcatccga tgattgtgat
      301 ctcctcggta ggtcggccct gtgacggtgc aatcgcgctg atggtcggca tcgcacaaca
      361 cacgggcatt tgaagcggcg gcatggtcgc gatcgttaag gacccggtcg gaaaattatt
      421 ggccgttgga ttcgtgaact ccacctgaca ggcgttctgg cgtgtaatag ctgccgcttt
      481 gaccgtacca agcgtgatat ccgaagccgt gatacgcccc aatccggaac cgattacggt
      541 gaccgttgca ccgccgggaa tattacagga tgggacaaaa gaacgaagtc tcggtgtatc
      601 acaggcatcg ccaagttcac cgccaggttc gttgccatcg caattaaact gatctgggtt
      661 cgacacggtc ggacagttgt cgatcttatc gttcactcca tcgccgtcac tatcacaggg
      721 tgccaattcg acgaaggttg aattgctgtc gttcgagctt tgtggatcgc ctgcgatcgg
      781 cgtcgtgatc gacgcttgaa caatatacgt cccccaggga aggttacgtg tatcccacgt
      841 aatgttaggt tcggtggcgg tcggcacgac gcgctccgcg ccggagggca aatctgtcac
      901 atgtgcaatc gatgtagaga gagattgtcc gcccgggcct aacgttctga cggtcacgtc
      961 aaacgtctcg tcactcaccc cgttattttt tgcaataaag cggatgttcg cttgatcgcc
     1021 cacccgcagc atggctggcg caacctgcac ctcaccaatg ccgacttcgt gttgtccgtt
     1081 gaagagggtg aaatcaccgg ta
//
LOCUS       DASTWE010001129         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_709077, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001129 DASTWE010000000
VERSION     DASTWE010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709077"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFT30_11810"
     CDS             <1..317
                     /locus_tag="VFT30_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5093365.1"
                     /translation="AKRKEMLAESIQLCHQLDGKGQYVHASPLQPEATGIVVRMRNGK
                     ATVTDGPFAETKEQLAGYFLIEAQDRDDAVRIAKLIPGARIGTVEVRPLREITGLPGE
                     EK"
     gene            471..680
                     /locus_tag="VFT30_11815"
     CDS             471..680
                     /locus_tag="VFT30_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093366.1"
                     /translation="MPNYKQRRKKDGRNKSKKKRAMRLSVEHRRANRQGPERRGPFKK
                     GLPDQPDIQEFKPGAANDKHSRKAA"
     gene            978..1451
                     /locus_tag="VFT30_11820"
     CDS             978..1451
                     /locus_tag="VFT30_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5093367.1"
                     /translation="MRYPIAPSVHSIPNGYHTVTPYLINENASRLIEFLKLVFDAKEI
                     NRLSGPDGRVMHAELKIGDSMVMVSDASGEWKPMPASIAIYVENVDATYQRALEVGAI
                     SLREPTDQFYGDRSAGVKDLAGNHWWIATHLEDVPPEELRLRAERWMKERTRVQV"
BASE COUNT          412 a          397 c          409 g          323 t
ORIGIN      
        1 aagccaaacg gaaggaaatg ctcgcggaat cgatccagct ctgccatcag ctcgacggga
       61 aggggcaata tgttcatgcc tccccactgc aacctgaggc gacaggaatt gtcgttcgga
      121 tgcgcaacgg aaaggcaacg gtaaccgatg gaccattcgc cgaaacaaaa gagcagctcg
      181 ccggctactt tctcatcgaa gcccaggatc gggatgacgc tgttcgtatc gccaagttga
      241 ttcccggtgc tcgtattgga acagtcgaag tcaggccctt aagagagatc accggcctgc
      301 cgggcgaaga gaagtgacca tgaccccaaa aacatccggc gcagtcatga caaccgaact
      361 tagcctaggg aagcccatgg cccttcttgt actttacctg caagaggttt caccagttgg
      421 atgaactctt ctcgaatagg agaattacat actgtctgaa aaggagcacc atgccaaatt
      481 acaagcaacg gaggaagaaa gacggccgca ataaatcgaa aaagaagcgg gccatgaggc
      541 tctctgttga gcatcgtcga gccaatcgac aaggccctga gagacgtggt ccgttcaaaa
      601 aagggctgcc ggatcagcct gatattcaag agttcaaacc cggtgcggcc aacgacaagc
      661 acagtcgcaa ggcggcatga gctcagcggg aaatgtttta aacctcctcg ctgagatgga
      721 aacacatgcc aataattgat caccgaattg gtgcactaag gtagtgcagc cgccgcgatc
      781 ggctctaggg aaaccccgcg atatctgtcg gcgatcccgg agatctcatt cgcggcaatg
      841 aacaatgttc gactctgcat cggtaagtcg gcctgactgg aaacgacaac cagtgcgacc
      901 atcacgagat tttcttgatt ccaattatgg agtagtccat gggtccacta ccgaggcaag
      961 acagtaatca ttcaaaaatg aggtatccga tagctccaag cgtgcactcg attccgaacg
     1021 ggtatcatac cgtcacgccc tacctgatca atgagaatgc ttcgaggctg atcgagtttc
     1081 tcaagctggt gttcgatgcc aaagagatca accgactatc cgggccggac ggtcgagtga
     1141 tgcacgccga gctgaaaatt ggagattcga tggttatggt gagtgacgcc tccggtgaat
     1201 ggaagcctat gccggcgtcg atcgccatct atgtcgagaa tgtcgatgct acctatcaac
     1261 gagctctcga agttggagcg atctcgctta gagaaccaac agaccagttt tatggcgatc
     1321 gtagcgccgg tgtgaaggac ctcgcaggca atcattggtg gattgcaact catcttgaag
     1381 atgttccgcc tgaagaactg aggcttcgag cagaacggtg gatgaaagag cggacccgcg
     1441 ttcaagtgta agtgctgttt atcgtttacc gcttccccaa cgacgttcat accattgtaa
     1501 cttttcccgt aactttccag cgctgctgtc ggatcacctc t
//
LOCUS       DASTWE010001130         1703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_159747, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001130 DASTWE010000000
VERSION     DASTWE010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159747"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VFT30_11825"
     CDS             complement(<1..139)
                     /locus_tag="VFT30_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEU5093368.1"
                     /translation="MRLFGIETEYGITRDDLPEMDPVVESMELVRAYLAASFEKRWDY
                     AG"
     gene            complement(228..>1703)
                     /locus_tag="VFT30_11830"
     CDS             complement(228..>1703)
                     /locus_tag="VFT30_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5093369.1"
                     /translation="TLLDVPGEGLARIVVGGAEYYANVDPRVSYTDLKVGTQILINEA
                     YAVIKTLGYDRNGPILKLTEAMPDGRLRFEQEMGRQSLILQRSTDLIGAELKAGDEIR
                     IDPSHHVAIEKLVDQKTSRHVLDETPKVTWEQIGGQSEAITAIRKAIEYPLLHAETFE
                     RFKFSQPKGFLLYGPPGCGKTLIGQAAAGSLAKLVSESNPEQPPDRDKRPPVTSGAFL
                     HVKGPEILNMWLGESERMVRDLFDQARARRRDGALPFIFIDEAESILGTRRAMRSFNI
                     SNTLVPMFCSEMDGIESLRDVVIILASNRPDLIDPAVLRPGRIDRKIKVSRPNKGAAA
                     EILKVYLTADLPLDPVLLKERGCEQSQAVASLVEDIIESIFRRTEDNRLLSIRLRTGQ
                     NKVLYRGDLMSGAILASIVQRAKEKAIDRTVQSGQQSGLQVKDLLDSVSEEFREGEML
                     PPDDAAEEWLKLLDHHPEQVVGISSFRRGRQTEERLVNQII"
BASE COUNT          382 a          515 c          403 g          403 t
ORIGIN      
        1 caccggcata gtcccaacgc ttttcgaaag aagcggcgag atatgcccgc accaattcca
       61 tcgattccac gaccggatcc atctccggta ggtcatccct cgttatccca tactcggttt
      121 cgatgccaaa aagacgcata gtttgttaga tgtaaaagat aaaccgtgaa acgagcaaca
      181 tccggacccc tcaatccttc atcttctatt tctcagattt catgtcttca gatgatctga
      241 ttcacgaggc gttcttctgt ctggcgccca cgccggaatg atgaaatccc gacaacctgt
      301 tccgggtgat gatccagcag cttcaaccac tcctccgcag catcgtccgg cggcaacatt
      361 tctccctctc gaaattcctc tgagacggaa tccagtaggt ctttgacttg caatccggac
      421 tgctgccccg actgaaccgt acgatcaatc gccttttcct tggcacgttg aacgatcgag
      481 gccaagatgg ctccgctcat caaatcgcca cggtaaagca ccttgttttg tccggtcctg
      541 agccggatcg acagaagccg attgtcctct gttcgacgga aaatcgactc aatgatgtct
      601 tcgacgagcg aggccactgc ttgactctgt tcgcaacctc gctctttcag cagcaccggg
      661 tccaacggca gatccgcagt caaatacacc ttcagaatct cggctgcagc gcccttgttc
      721 ggccgactga ccttgatctt ccgatcgatc cggcctgggc gcaacaccgc cggatcgatc
      781 aagtcagggc ggttggaagc caatataata acgacatcac gtagcgattc gattccatcc
      841 atttcgctgc aaaacatcgg cacgagcgtg ttcgagatat tgaaagaccg catagcgcgt
      901 cgcgtcccca aaatcgactc cgcttcgtcg atgaagataa acggcaacgc gccgtccctc
      961 cgtctggcac gtgcctggtc gaagagatcg cgaaccatcc gttccgattc acccaaccac
     1021 atgttgagaa tttcaggccc tttgacatgc aggaatgctc cgcttgtcac cggcggacgc
     1081 ttgtctcgat cgggtggttg ctctggattg gattcgctga ccagcttagc caagctacct
     1141 gccgccgctt gtccgatgag cgtcttccca catccgggtg ggccatagag aagaaagccc
     1201 ttcggttggg aaaacttgaa ccgctcaaag gtctccgcat gcaatagtgg atactcgatc
     1261 gcctttctga tggctgtgat cgcctcgctc tggccgccga tctgctccca cgtgaccttt
     1321 ggcgtttcat caaggacatg ccggcttgtt ttctgatcca ccagtttctc gatcgctaca
     1381 tgatgactgg gatcgatgcg aatttcatcg cccgccttga gctccgcgcc tatgagatca
     1441 gtcgacctct gcaaaatcaa cgactgccgg cccatctcct gttcaaaccg caatcgtcca
     1501 tcaggcatgg cctctgtcag tttcaagata gggccgttac gatcataacc aagcgttttg
     1561 atcactgcgt aggcttcgtt gatcagaatc tgcgttccaa ccttaagatc cgtataggaa
     1621 acgcgggggt ctacgttggc ataatactcg gccccaccga caacgatgcg ggcgagtccc
     1681 tcgccaggaa catcaagtaa agt
//
LOCUS       DASTWE010001131         1158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1290519, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001131 DASTWE010000000
VERSION     DASTWE010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290519"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFT30_11835"
     CDS             <1..400
                     /locus_tag="VFT30_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093370.1"
                     /translation="CNSNTPEPASVELASPRLRLTIVRMATDPFLQRFNLTLNVKGAS
                     GCSSSTELFPDTGYAGRRNVYQASNERVYVVGQYDARVIDALSCRTSLAEFRHLDRNV
                     VFIGSFDQDESKHWTYVSAAQRPELPFEKR"
     gene            complement(440..592)
                     /locus_tag="VFT30_11840"
     CDS             complement(440..592)
                     /locus_tag="VFT30_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093371.1"
                     /translation="MPHVLSMTTGQIGNPIAIVILVISDDGLLNVVMILVLQEGCWTG
                     KLRSGH"
     gene            944..>1158
                     /locus_tag="VFT30_11845"
     CDS             944..>1158
                     /locus_tag="VFT30_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093372.1"
                     /translation="MLFLYSALFFTLAFYGAHFKLLLTLYSSDSLEERQVAAVEAEVA
                     LAEINRLETLYHTNHGTYSGDVTAIGFS"
BASE COUNT          275 a          301 c          289 g          293 t
ORIGIN      
        1 gtgcaacagt aatactcccg agccggcttc ggttgaacta gcatcaccac gacttcggct
       61 gacgattgtg agaatggcga cggatccatt cctccagcga tttaatctca ctctgaacgt
      121 caagggagcg agtggctgtt cttcttcaac cgaattgttt ccggataccg gttatgccgg
      181 tcgacgtaac gtctatcaag cttcaaacga aagagtctat gtggtcggac aatatgatgc
      241 gcgcgtcatc gatgcactga gctgccgaac gagcctcgca gaatttcggc atctcgatcg
      301 aaacgtcgtt tttatcggca gctttgatca agatgaaagc aagcactgga cctatgtttc
      361 tgccgctcaa cggccggaac tgccatttga gaaacgttga cgttggcttg gatccagaag
      421 aattgctgaa gaaacaatat cagtgacctg atcgtagttt tccagtccag cacccttcct
      481 gcaagacgag aatcattacg acattcaaca gcccatcatc ggatatcacc aagatgacta
      541 tggcgattgg attgccgatc tggcctgtgg tcatggacaa cacgtgcggc atcaacctcc
      601 attcacgaat cgctcttggg tactcactgt cgccggccgt gaacagtatt tgggtacccg
      661 tttgaattgt aagaagtgcg aggaacctgc cgattctcct ccgacatcgg gataaattct
      721 gcaccgtccg ctctcctcca tcacgaacgc tccatttact tggccgatgg cagctcaatg
      781 ataccgtgaa tcgcagagct attggggagt gtcatagaca ggcccaatga aggaaggatt
      841 gcgtatggct accgttgcaa aacctgcact atccagcgtc ggccgtaaag acgggttttt
      901 catccatcgg gtccagaagg gctatgccgc atagattaga ttgttgctgt tcctctactc
      961 ggccttgttc ttcacattgg ctttctatgg cgcccatttc aaactcctgc tgacactcta
     1021 tagcagtgat tcattggagg agcgtcaggt tgccgctgtg gaggcagagg tggcgttggc
     1081 cgagatcaac cgccttgaaa cgctctacca taccaatcac ggaacatatt ctggtgatgt
     1141 cacggcgatc ggtttttc
//
LOCUS       DASTWE010001132         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_741218, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001132 DASTWE010000000
VERSION     DASTWE010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741218"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFT30_11850"
     CDS             complement(<1..264)
                     /locus_tag="VFT30_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5093373.1"
                     /translation="MPLDPEKLRHAMRAWTTGVAIVTSIYEDQQYGMTVNSFTSISLE
                     PPLISVALRQLTHTHELVVKSGMFSVTILTATQKDLSDRFAGKL"
     gene            486..>1087
                     /locus_tag="VFT30_11855"
     CDS             486..>1087
                     /locus_tag="VFT30_11855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5093374.1"
                     /translation="MQTILGVAILLATLFTALPSRGLVGGNFYDRLSVILTPRTNEQE
                     QIPFAPQAPAQLRLGIVAGHSGNDSGAVCLDGNGNVTLTEADVNLKIAAMVQEQLIQE
                     GYQVDLLREYDSRLNGYRALAVVSIHNDSCEYVNDEATGFKVAAALNTNDLNRANRLT
                     ACLVDRYQNITAMNFHAGSITPDMREYHSFREIDSSTVAA"
BASE COUNT          263 a          298 c          286 g          240 t
ORIGIN      
        1 cagctttccg gcaaagcgat ccgagaggtc cttctgtgtc gcggtcaaaa tcgtgacgga
       61 gaacattcca gattttacga ccagttcatg tgtatgagtt aattgtcgca aggcaacaga
      121 gatcagcgga ggctccaacg aaatggaggt aaatgagtta acggtcatgc catattgctg
      181 gtcttcataa atggaagtga cgatcgccac acccgttgtc cacgcccgca tggcatgccg
      241 aagtttttca ggatcaagag gcatataaat tctccaggaa attcatgata acgaggaggg
      301 gtaggtgcgt caagtcaaaa aacaaaatcg gaaagcattg tgatccctgc tcgagttacg
      361 gatttgtaac aggaaattcg cggcttcctc ccttgcaagt tcatgcctta aaaggtacta
      421 ttcggatata cgatatggaa accgcttctc ggcctcccag gcgtcgtaca cgctcgttcc
      481 gggcagtgca gacgattctc ggcgttgcca tccttctggc gaccctcttt acggcgctgc
      541 cttcgcgtgg actggtggga ggcaatttct acgatcgatt aagtgtcatc ctcactcccc
      601 gtacaaatga acaggagcag atacccttcg ccccgcaggc accagcgcag ctgcgtctgg
      661 gaattgtcgc agggcactcg ggcaacgact ccggagccgt ctgcctggac gggaacggca
      721 atgtcacgct caccgaagca gacgtgaacc tcaagatcgc ggccatggtc caggagcagt
      781 tgatccagga gggatatcaa gtggacctgc tgcgcgaata cgactcgcgt ttgaacggct
      841 accgcgctct cgccgtggtc tccatccaca acgattcctg tgaatacgtc aacgatgagg
      901 cgaccggttt caaggtagca gcggcgctca acacgaatga tctcaatcgc gccaatcgtc
      961 tcactgcctg tctggtggat cgttaccaga acatcacagc gatgaatttt catgccggca
     1021 gcatcacccc ggacatgcgc gaatatcatt ccttccgcga gatcgactcc agtacggttg
     1081 ccgccat
//
LOCUS       DASTWE010001133         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1484769, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001133 DASTWE010000000
VERSION     DASTWE010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484769"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1183
                     /locus_tag="VFT30_11860"
     CDS             <1..>1183
                     /locus_tag="VFT30_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093375.1"
                     /translation="NEFHGSLYEYHRNTVLNARQFGIAQRPKDIEHDFGGTIGGPIKI
                     PWLAWTDRKKTYGFFNYEQFIRRGGVTAPTVSIPSIKQRAGDFTDWRDASGNLIPVYD
                     PATTRRDPNTGQFVRDQFMGCDGRTPNVICASDPRMQNSLAKQWFRYLPTPTNDLPLN
                     NYTLPVPITGSVNSDTILWDIRIDHHVGDKDRFFYTQHYYASEANVNETVLPKEISAN
                     GYRKPNTNNLVRGSWSHTFNPNLLLNVNGGYNNIYSVSVCVDRDYADAVPKIAGVVHN
                     NLPPVIGLQDFAGMGCNSESSQTRPAYIGNALLSWVNGRHTWKFGGEYRNQQVNTTTY
                     GNEPGSFSFNRLNTGLLGVNSGSSVASFLLGAVSSGSVAVRTQGSNYARQPYTAVYFG
                     DT"
BASE COUNT          280 a          328 c          306 g          269 t
ORIGIN      
        1 aaatgaattt catggatcgc tgtatgagta tcaccgcaat acggtactca atgctcgtca
       61 gtttggtatc gcccaacgtc ccaaggacat tgagcatgac ttcggaggca cgattggcgg
      121 acctatcaag attccttggc tcgcctggac cgatcgaaag aagacctatg gctttttcaa
      181 ctacgaacaa ttcattcgtc gtggcggcgt cacggcgccc acggtgagca ttccatcgat
      241 caagcagcga gcgggagact tcaccgactg gcgcgacgca tctggcaatt tgattccggt
      301 ttacgacccg gcaacgacgc gacgcgatcc caacacaggc cagttcgttc gtgaccagtt
      361 catggggtgc gatggcagaa cccccaatgt gatctgtgcc tccgacccgc gaatgcagaa
      421 ttccttggcc aagcaatggt tccgatactt gccgaccccc accaatgatc tgccactaaa
      481 taattacacg cttcctgtcc cgattactgg cagtgttaac agtgacacca ttctttggga
      541 cattcggatc gatcaccacg ttggggacaa ggatcgcttc ttttatacgc agcactacta
      601 tgcctccgaa gcaaacgtca acgagacagt ccttccgaag gagatctctg cgaacggcta
      661 tcgcaagccc aacacgaaca atctcgtgcg aggcagctgg agccatacct tcaacccgaa
      721 cctgcttctg aatgtaaacg ggggatacaa caatatctat tcggtttctg tgtgtgtgga
      781 tcgcgactac gccgacgctg taccgaagat tgcgggcgtc gtccacaaca acttgccgcc
      841 tgtgattggc ttgcaggatt ttgcgggtat gggttgcaac agtgaatcca gccaaacgcg
      901 gcccgcctac atcggcaatg ccttgctctc ctgggtgaat gggcgtcaca cctggaagtt
      961 cggtggtgag taccgcaacc agcaggttaa caccaccacc tacggaaacg agcctggtag
     1021 tttcagtttc aaccgactca acaccggact gttgggagta aacagtggga gctcggttgc
     1081 cagttttctt cttggggctg tgagcagcgg aagtgttgcc gtccgaaccc agggctcaaa
     1141 ctatgcaagg caaccttata ctgcggttta ttttggggac act
//
LOCUS       DASTWE010001134         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1484807, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001134 DASTWE010000000
VERSION     DASTWE010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484807"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..409
                     /locus_tag="VFT30_11865"
     CDS             185..409
                     /locus_tag="VFT30_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093376.1"
                     /translation="MDTRVRDYLIGAHNKVIGHYRHLLEAGSLAPPERDRIQRGLARI
                     EAELDAIVTGPSASRRLVQAQEPPPRMASY"
     gene            437..628
                     /locus_tag="VFT30_11870"
     CDS             437..628
                     /locus_tag="VFT30_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093377.1"
                     /translation="MKSGENEGFDVVINGENKYFADLELSAIACAGFHKEHSPEDTVQ
                     IRTRASGVLRTVLGHARLQ"
     gene            880..>1219
                     /locus_tag="VFT30_11875"
     CDS             880..>1219
                     /locus_tag="VFT30_11875"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HEU5093378.1"
                     /translation="MSHGVHAVQHEVEHRIHHDDHNDRTNGKNLNKKVALLISVLALF
                     LAFSETLGKSAQTAALNSQIEASNLWSFFQAKNIRRTSLLIATERSKLDLIGVADPDQ
                     KTAIQKQIDDW"
BASE COUNT          305 a          326 c          334 g          254 t
ORIGIN      
        1 gcaccggcta tgtcttaaaa agcactccgg agtgagtttc cagacacgga aactgctgcg
       61 tcaggtcacc ggaaggaccg catcgaaatt cacgccgggt ggcgcgaaaa aagagtaact
      121 cgccccatcc cgtctttgcc gaagctcaaa tgccttgaag atacggaatc caaggaggac
      181 acccgtggac acgagggtgc gagattatct catcggagct cataacaagg tcatcggtca
      241 ttaccgccac cttcttgaag ccggatctct cgcaccgccc gagcgagatc gcattcaacg
      301 aggcttggcc cgcatcgaag cggagctaga cgctatcgtg acaggcccat ccgcctccag
      361 gcggctcgtt caagcccaag aaccaccgcc tcgaatggct tcctattgaa attgctcgcg
      421 cgattaggag ggtgttatga aaagcggaga gaatgaaggc tttgacgtcg tcatcaatgg
      481 cgaaaataag tattttgcgg acttggagct cagcgctatc gcgtgcgcag gctttcacaa
      541 agaacattct cccgaggaca cggtgcaaat acgaacacgc gcgagcggtg tcttgcgcac
      601 tgtgttaggg cacgcgcggc tacaataggc tcgtgtgtca aatacgacgg agtcgagtgg
      661 gcattggctt gaaactcttt ccggctttct gaattattca tacgatgcgg cgctcgcttc
      721 ccggcctgtt gctaaggcaa aaaacccggc ccccaggcaa gcgccgcttc agcgttatga
      781 cacggtcacg gacaagccgc tcagcctgca ttggaggttg gagggctact tgctcggata
      841 agacttgttg caaaggggcg cctgaggaca ttggacgata tgagccatgg tgtgcacgcc
      901 gttcaacatg aggtcgagca tagaatccat catgacgacc acaatgatcg gacgaacggc
      961 aaaaacctta acaagaaggt tgcgctccta atttcggtgc ttgcgctgtt ccttgcattc
     1021 tctgaaactc tgggtaagag cgcgcagaca gcagcattga actctcagat cgaagcttcc
     1081 aacctttgga gtttcttcca agccaagaac atccgccgga cgtccttact gatcgcaacc
     1141 gagcgttcga agctggatct gataggtgta gcggatcccg atcagaagac tgcgatacag
     1201 aaacagatcg atgattggt
//
LOCUS       DASTWE010001135         1385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1484817, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001135 DASTWE010000000
VERSION     DASTWE010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484817"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..766)
                     /locus_tag="VFT30_11880"
     CDS             complement(374..766)
                     /locus_tag="VFT30_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093379.1"
                     /translation="MRIGQLIRSLVLAGSVLIWLQATSVQAVGEPYALGYDVIGQAEK
                     VLKEAATNSQKWQSKQDFIQRDIYDITFLLEQACRAADKANDAAMKDSAHQALSLLQR
                     AVARGHFDPDKVEPIFTLIRQLLPNVSV"
     gene            complement(817..1251)
                     /locus_tag="VFT30_11885"
     CDS             complement(817..1251)
                     /locus_tag="VFT30_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5093380.1"
                     /translation="MSWENQLPVLFHNGSIESQIDRLLDDAIQSVNRWPQSWDRAYNM
                     FEDEQDFTVQIALPGLDVNQIDVQVENNILRVKGERKDDESEGRQWYTRGIEGCAFSC
                     SFKLPEHADYEKSTASYKQGLLTITLPKREEAKPRRIMIESQ"
BASE COUNT          306 a          408 c          324 g          347 t
ORIGIN      
        1 caaaccacct caggactttt actttccatt ctttccattg aaaattcagc atctgaaatt
       61 tcatcatggc acctccctgt ttcattcctc tccaccacac tcgaatctca ctaggccgcg
      121 gccttgagag gcggatagcg agtgccatcg gattgacgtc ctgtctcctc ggaggacgtg
      181 gacacggtgg tggattccga tgacaacgtc gaaatcccta tcaaaaccac tgcaatgacc
      241 ccaccgaacg ccgggcttgc atctatcaat gcgctcatat ccatcatcgt ggtcacctcc
      301 tttccgtttt gtcataagaa gtccactttc tcgtctgcaa gactcggctt attccagctt
      361 tatcccttca gctctacacc gacacattgg gcagcagttg cctgatcaat gtgaaaattg
      421 gctcaacttt gtccggatca aagtggccgc gagcgacagc ccgctgcagc aaggagagcg
      481 cctggtgagc tgaatctttc atggccgcgt cattcgcctt atcagccgct cggcaggctt
      541 gctccagcaa aaacgtgatg tcatagatat cacgctgaat gaagtcctgc ttgctctgcc
      601 acttctgact gtttgtggcg gcttctttta ataccttctc ggcttgaccg atcacgtcgt
      661 agcccaacgc gtacggttca ccgacagcct gaaccgatgt cgcttgcagc catatcagga
      721 ctgaaccagc aagcacgaga ctcctgatca attggccgat ccgcatggcc ttcacctcac
      781 ccccgaggac tcgccggcga gccgatgcac cagcgcttac tggctctcga tcatgatccg
      841 acgcggtttt gcctcttccc gtttggggag agtgattgtg agaagtcctt gcttatatga
      901 ggccgtcgat ttctcatagt ccgcgtgttc gggaagctta aatgagcatg agaacgcaca
      961 tccctcgatc cctcgcgtat accattgccg tccctcagac tcgtcatcct tccgttcacc
     1021 tttcacgcgg agaatgttgt tttctacctg cacatcgatc tgattcacgt ccagtcccgg
     1081 aagggcaatc tggaccgtga agtcctgttc gtcttcgaac atgttatagg cccggtccca
     1141 tgattgcggc caccgattca cagattggat tgcgtcatcg agcagacgat caatctgcga
     1201 ctcgatcgac ccgttatgga acaacacagg caattggttt tcccaagaca taggaaacct
     1261 ccttgtgctt gtgaatgtat agatgattat ggacaaatga gctggaacga gaggactgcg
     1321 tgaagggccg agcgtacacc gctatgacag ctggccagac agtgggccct tcaccagtcc
     1381 atcca
//
LOCUS       DASTWE010001136         1274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1420484, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001136 DASTWE010000000
VERSION     DASTWE010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420484"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..594
                     /locus_tag="VFT30_11890"
     CDS             124..594
                     /locus_tag="VFT30_11890"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU5093381.1"
                     /translation="MPTDELSIQRFQTVLMKSGSRVFIPIPCSPNDAWGVKQRHYIRG
                     TVNGHAVRGSLGSDGKQYFLPLGAAWRRDCGLDAGDQVDVSLSPEGPQSENLSTDIAS
                     ALDAEPQAKTFFESLATFYRNTYIKWIESAKRPETRAARIQEMIELLNAGKKQK"
     gene            591..>1274
                     /locus_tag="VFT30_11895"
     CDS             591..>1274
                     /locus_tag="VFT30_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5093382.1"
                     /translation="MTVDQLTGEFETFQRELKSFLLRMTASVQDAEDIVQETYLKAHA
                     RIDTFRGESSLKTWMFSIASNLARDLLRSRKRWPETVTDICREETLGNRQFFQEAMHI
                     RQTSPQGNFEIREHIAFCFTCISKSLPLEGHLVLLLKELYGFKVKEISQILQLSEAMV
                     KYHLHVSRSKMMEIFDHRCSLINKEGICYQCTELNGIFNPKQNAQQELLKIEMAREAE
                     NRNRDELFDL"
BASE COUNT          337 a          329 c          321 g          287 t
ORIGIN      
        1 ggtgaatgcc ggtgggagtt tttgattcct ggggaatctg ctatactttt caaatctcaa
       61 ggaccatgac atagcaccgt catacggata caatcatggc aaccgtttac aagtaatttt
      121 ccaatgccaa ccgatgaact gagcatacaa cgtttccaaa ccgttctcat gaaatccgga
      181 tccagggtat ttatccctat cccttgtagc cccaatgatg cctggggcgt caagcagcgc
      241 cattacatcc ggggtaccgt taacggtcat gccgtgcgcg gctcgctcgg ttccgatggc
      301 aagcagtact ttcttccttt gggcgcggcc tggaggcgtg actgcggtct cgatgccggc
      361 gatcaggtag atgtctcttt atctccggag ggaccgcagt ctgaaaatct gtcaacggat
      421 atcgcgagtg ccctggatgc cgagccgcaa gccaaaacct tttttgagtc ccttgccacc
      481 ttttatcgaa acacctacat caaatggatc gaaagcgcca aacgacctga aacgcgtgct
      541 gcacgcattc aggaaatgat cgagttgctc aatgcgggca aaaagcaaaa atgacagtcg
      601 atcaactcac cggcgagttt gagacatttc aacgggaatt gaagtccttc ctcctgcgaa
      661 tgaccgccag cgttcaggat gctgaggaca tcgttcagga aacctatctc aaagcgcacg
      721 ccaggatcga tacgttccga ggagaatcat cgctgaagac ctggatgttc agcatcgcct
      781 cgaacctggc cagggacctg ctgaggtcca ggaagcgctg gcccgaaacc gtgaccgaca
      841 tctgtcggga ggaaactttg gggaatcggc aattcttcca ggaagcgatg catatcaggc
      901 agacatcgcc acaggggaac tttgagatca gggagcacat tgccttttgt tttacgtgca
      961 tctccaaatc tcttccgttg gaagggcatc tcgtcctgct attaaaggaa ctctacggtt
     1021 tcaaggtcaa agagatttcc cagatccttc agctgagcga agcgatggtg aaataccacc
     1081 tgcatgtcag ccgcagcaag atgatggaga tctttgacca ccgctgttcc ttgatcaaca
     1141 aagagggtat ttgttaccaa tgtacggaac tcaacggtat cttcaatccc aaacaaaacg
     1201 cacagcaaga actactgaaa atcgagatgg caagggaggc ggaaaacaga aaccgggatg
     1261 aactcttcga cctg
//
LOCUS       DASTWE010001137         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_935469, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001137 DASTWE010000000
VERSION     DASTWE010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935469"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFT30_11900"
     CDS             <1..707
                     /locus_tag="VFT30_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEU5093383.1"
                     /translation="PESRRGNRKSTFGREGGTMRNLTKAILLVMVLLSLQACGTTIEP
                     GQRGVRWNPLTGGLSPEPLKSGFYWRAPWNQIYLYNVRWRSYTETIDALSSDDLPVTL
                     RTVVVIRPIPEELYFLAKEIGPDFYPRVAKRELLAAVRSVVSNYPMATVLEHSSEIAS
                     EVEAVVVEKLKDRHVQVASVAMAGLDPEYIRFKLYDSSHSKWVLLPEKQNVPLLINPG
                     TDQAITSSHEDFRARH"
     gene            815..1429
                     /locus_tag="VFT30_11905"
     CDS             815..1429
                     /locus_tag="VFT30_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HEU5093384.1"
                     /translation="MRTIGLFVMSVLLAACTATPVFPPDLTRDIKTDPGVLKAWKEQT
                     SYPSGANFSSHKVQLGGQITQVIWKPEGVVILAEEQPIDKYLGYGPTSVRRGGSFEFA
                     IVFNGLPNADALQVGNQLAVVGTTDRARPETIGWMPRVIPHLRAQCLDIWKTQGFETD
                     NIIYQGSMGYYPLEKRTFCQEEGKGDSLSTGGGQGNQDTSSTGS"
BASE COUNT          438 a          479 c          486 g          379 t
ORIGIN      
        1 ggccggaatc tcgccggggc aatcggaagt ccacgttcgg gcgagaagga gggaccatgc
       61 gcaatcttac gaaggccata ctgcttgtca tggtattgct ctccctgcaa gcctgcggga
      121 ccaccatcga acccggtcaa cgaggcgtac gatggaatcc cttgaccggt ggtctgagcc
      181 ccgaaccact taagagcgga ttctattggc gggcgccatg gaaccaaatc tacctgtaca
      241 acgtgcgatg gcgcagttac accgaaacga tcgatgccct aagctcagat gacctgccgg
      301 tcacgctcag gaccgtcgtt gtcataagac cgattcccga agagttgtat ttcttggcca
      361 aggaaatcgg accagatttc tatcctcgcg tcgccaaacg cgagttactg gccgccgtcc
      421 ggagcgtcgt ctccaactat cccatggcca ccgtgttgga gcacagttct gaaatagcca
      481 gcgaggtgga ggcggtggtg gtggaaaaac tcaaagaccg tcatgtccaa gtggcgagcg
      541 tcgccatggc cggtctcgat ccggagtaca tcaggttcaa gctctacgac agctcacact
      601 ccaagtgggt actgctaccg gagaagcaaa atgtgccgct tttgatcaac cctggaactg
      661 atcaggcaat aacttcgtcg catgaggatt tccgtgcccg acactgactc aggcatgcga
      721 tcaatactcc caatggaatt catccaagaa agccaagcga gagctgcacc gcctcatttt
      781 gcgctaggtt tgcgctagat aaggagagat tcacatgcgt acgataggcc tgttcgtgat
      841 gtctgtatta ttggccgcct gcacagccac accggtgttt cctccagacc tcacgagaga
      901 tatcaaaacg gaccccggtg tcttgaaggc ctggaaggag cagacatcct atccttccgg
      961 cgccaacttc agctcccata aagtgcaatt gggcggacag atcacgcaag ttatctggaa
     1021 gccggaaggt gtcgtcattc tcgccgagga gcagccaatc gacaaatatc tagggtatgg
     1081 ccctacgagt gtgagacggg gaggctcctt cgaattcgcg atcgttttta acggccttcc
     1141 aaacgccgat gcgttgcagg tcggcaacca gcttgccgta gtcggaacga cggatagagc
     1201 cagaccagaa acgatcggtt ggatgccgag agttatcccg caccttcgcg cccaatgtct
     1261 tgacatctgg aagactcagg ggtttgaaac agacaatatt atctatcaag gctcgatggg
     1321 atattatccg ctggaaaaac gaaccttctg tcaggaagaa ggcaaaggag acagcttgtc
     1381 aaccggtggt ggtcaaggta atcaggacac gagctccaca ggctcgtgaa tgggagccga
     1441 agagttggtc cggaattgag ccaaccttca gtgagggcaa tcgatgatta atctcgttgc
     1501 cggatgttta tcttgtacct cgagacggga acattggagt cggcattagc cggctgtacc
     1561 gccgataccg agagtttcaa tccgaacgtc tcacgcatag ctctcgacat gctcaaatag
     1621 gcgcacgcgg tggagctatt tatgtgtgcg aagcggagac tcattctctg caccggttta
     1681 ccgcagaaag gggggccttc atcacatgcg tactatggtc ccatcataac gtgaagattg
     1741 ttcactgcct gcacaacgga aagcgcacct gctcctcaac tc
//
LOCUS       DASTWE010001138         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_611832, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001138 DASTWE010000000
VERSION     DASTWE010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611832"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFT30_11910"
     CDS             complement(<1..1244)
                     /locus_tag="VFT30_11910"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5093385.1"
                     /translation="MKKEVRLQSHELLVGAGRAPARAMLKAVGFTDDDLSKPLVGVAN
                     TWIEVMPCNFHLRRLSERVKAGIRAAGGTPIEYNTIAVSDGISMGTEGMKASLISREV
                     IADSIELVARGHLFDAVVALSGCDKTIPGTVMALARLNVPSLMLYGGSIMPGQFQGHD
                     VTIQDVFEAVGKHASGKMTDAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGISPM
                     GRNGVPAMDGRKDDVAFECGKMVMELVKNDLRPRRIITRKSLENAIAAVATTGGSTNA
                     VLHLLALARESGIKLSIDDFDKINRKVPLLADLKPGGRFAAADLYAAGGTTLVAKRLL
                     HAGLLHGSQPTVTGRTIGEEASHARETPGQQVLRPLSHPIKPTGGLVILKGNLAPEGC
                     VVKVAGHAMTKFQGPAKVYDRE"
     gene            complement(1262..1906)
                     /locus_tag="VFT30_11915"
     CDS             complement(1262..1906)
                     /locus_tag="VFT30_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093386.1"
                     /translation="MTAITKTTTAFAVASACLLMMGIPSLWANDPSYGHGGGIHGVGG
                     HGYGKGMMHSRTGHLIRHLLKHEKDIGLTADQVTKLKDIQLNLDKIRIKAEADIQIAE
                     RELKALTESEKSDLAAIEAKLRQSEDLQVGLRMTSIKTRRDVMGLLTPEQRAKEKSEH
                     DKVMDQHKGYGSSHGGALPYGANPHGANTYGTNPHSKNPHEAVPPAPPSSMSVQ"
     gene            complement(2016..>2210)
                     /locus_tag="VFT30_11920"
     CDS             complement(2016..>2210)
                     /locus_tag="VFT30_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093387.1"
                     /translation="AEGKLRLKGKGLPSATGGHGDLFLTLQIVLPAGISEDERVLYER
                     LSKQRHPDPRTELLYGAQRR"
BASE COUNT          430 a          645 c          600 g          535 t
ORIGIN      
        1 tcttctcggt catagacctt cgccggtccc tggaacttgg tcatcgcatg gccggctacc
       61 tttaccacgc aaccttccgg cgccaaattg ccctttaaaa tgacaaggcc gcctgtcggt
      121 ttgatggggt gggagagggg acgcaagact tgttgtccgg gcgtctcacg agcgtgcgat
      181 gcctcttcac cgatcgttcg acctgtaacg gtgggctgac tcccatgtag gagtcctgca
      241 tggagcagac gttttgcgac cagggttgtg ccgcctgcgg catagagatc ggctgcagca
      301 aagcggcctc ccggcttgag gtcggccaat aagggaacct tgcgattgat cttgtcgaag
      361 tcgtcgatgc tcagtttgat cccggactca cgggcgaggg cgaggagatg cagcacggca
      421 ttcgtcgagc cgccggtcgt ggcgacggca gcgatggcgt tctccaacga ctttcgggtg
      481 ataatccggc ggggacgcag atcattcttc acaagctcca tcaccatctt gccgcactca
      541 aacgcgacat catctttccg accgtccatg gcgggtaccc cgttgcggcc catcggtgaa
      601 atgccgagga attcaaatgc aatggccatt gtgttggccg tgaactggcc tccacaggct
      661 ccgggtcccg ggcaagcatg atcttccaag tccttcagct cagcgtcggt catctttccc
      721 gacgcatgct tgccgaccgc ttcaaagaca tcctgaatgg tcacatcatg tccctgaaac
      781 tgtcccggca tgatcgagcc gccatagagc atgagcgacg gcacgttcaa tcgggcgagg
      841 gccataacgg ttccgggaat tgttttatcg catccggaca gggcgacgac ggcatcgaac
      901 aagtgtccac gcgcgacgag ctcgatcgaa tccgcgatga cctcgcggct gattagggaa
      961 gccttcatcc cttcggtgcc catcgagatc ccatcggata ccgcgatggt gttgtattca
     1021 atcggagtac cgccggccgc tctgatcccg gctttcactc gctcggacag ccgccgcaaa
     1081 tgaaagttgc atggcatgac ttcgatccat gtattggcca cacctaccag cggtttcgag
     1141 agatcatcgt ccgtaaagcc gacggctttt aacatggccc tcgccggtgc acggccggcc
     1201 ccgaccagaa gttcatgact ctgaagtcgc acttcctttt tcatcagtct tcgtggtccc
     1261 attactgtac agacatgctg ctcggtggtg cagggggtac ggcctcatga ggattcttgc
     1321 tgtgaggatt ggtgccatat gtatttgcac cgtgaggatt cgcaccatac ggcagtgcgc
     1381 ctccatgaga tgatccgtag cctttgtgct gatccatcac tttgtcatgc tcagactttt
     1441 ccttcgcgcg ctgttcaggg gtcagcaagc ccatcacatc acgacgtgtc ttaatggagg
     1501 tcatccgtag cccgacctgc agatcttcac tttgtctcag ctttgcttcg atcgcggcga
     1561 gatcagactt ctcactctct gtcagtgctt tcagctcacg ttcagcgatt tggatatcgg
     1621 cttcagcctt gatgcgtatt ttgtccagat tcagttggat gtctttcagt ttggtaactt
     1681 gatcggcggt gagtccgatg tccttctcgt gtttcaatag atgtcggatc aggtggccgg
     1741 tcctgctgtg catcatccct ttgccatagc catgtcctcc gacgccatgt atgcctccac
     1801 catggccata gctcggatca tttgcccaca ggcttggtat gcccatcatc aacagacatg
     1861 ccgacgctac ggcaaacgca gtggtcgtct tggttatagc cgtcatgaat tcctccttgg
     1921 ttgagaggcc ctggggccaa agcttgccat agcagtcaac gcaacgcaag cgagtctctc
     1981 gactcaaaac tagtcgaagc cgatcgagtt ttggctcaac gccgttgggc gccgtagaga
     2041 agctccgttc gcggatccgg atgccgttgc ttgctcaaac gttcgtacaa tacccgttca
     2101 tcttcgctga taccggctgg aaggacaatt tgcaacgtca ggaaaagatc gccatgaccg
     2161 cctgtggctg agggaagccc cttccccttg agccgtagtt ttccctcggc
//
LOCUS       DASTWE010001139         1399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_967850, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001139 DASTWE010000000
VERSION     DASTWE010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967850"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..>1399)
                     /locus_tag="VFT30_11925"
     CDS             complement(15..>1399)
                     /locus_tag="VFT30_11925"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU5093388.1"
                     /translation="AKGTIHFGDRFMIDMAVATSTLSVSSLPEWVSKSWLEAGNFELS
                     LDVKGRDTDWKAWRISGWMGLTNGLMVAKGIEGPIQDLYARVRFVRNEVELKRLSFKV
                     QGSDIAVEAAIRNWPVKPTITGRIESNQLDISLVIPKGERTPIREFLETLAATSQVTM
                     SVAITRGRYKHLKFSSLAARINIQDGVLDIDRLSGESAHGHVAGRLVVHLPPKAPADL
                     DLSFRATGMEFEDLLRLTKAHAHGVSGEVRVSGVLRGHGRNPHGVYPSLNGRVELLME
                     NGRILKSNEQALWKIISLLNLPAVLQGKVDLEKEGLPYNRISATVTIQNGMFQTENLI
                     IDSPILKITAAGNYDLPTDQLDLAVAVSPFGSYSQFLKTIPLFGRIVAGDRKGIATAM
                     FTMKGAMEDPEVTYLPVKSFASGLSGLAQLAVDVLTNTLTLPIDLVTPDEESEVRPKD
                     MTLSPAPIMP"
BASE COUNT          325 a          407 c          333 g          334 t
ORIGIN      
        1 agaatcaagg ctggtcacgg cataatagga gctggcgaga gagtcatatc ttttggtcgc
       61 acttcggatt cttcatctgg cgtcaccaaa tcaatcggca atgtcagggt gtttgttaag
      121 acatcaaccg caagttgggc caaccccgat agaccggacg caaaggattt caccggcaaa
      181 taagtcactt ctggatcctc catcgctccc ttcatggtaa acatcgcggt tgcaataccc
      241 ttgcgatcgc ccgcaacgat tcgcccaaag agtggaatcg tcttgaggaa ttgtgagtat
      301 gacccgaacg gactcacagc aacggcaaga tccagctggt ctgtcggcaa gtcgtaattc
      361 ccggccgccg taattttcag aattgggctg tcaatgatca gattttccgt ctgaaacatc
      421 ccattttgaa ttgtaaccgt cgcggaaatt ctgttgtatg gcaacccctc cttttcaaga
      481 tcgacctttc cctgcaacac agccgggaga ttcagcaaac taatgatctt ccatagtgcc
      541 tgctcgttcg atttcaggat gcgaccattt tccatcaata actcgactct cccgtttaac
      601 gaagggtaga ccccatgggg attgcgccca tgaccgcgaa gaacgccact gacgcgaact
      661 tcccctgata caccatgagc atgagccttg gtcagcctca acagatcttc gaattccatt
      721 ccggtagccc ggaatgaaag gtccaggtcg gctggtgcct tcgggggcag gtgcacaacc
      781 agccgcccag ccacgtgccc atgggctgac tcgccggaaa gacgatcgat gtccaacacc
      841 ccgtcctgaa tattgattcg agcggcaagc gagctaaatt tcagatgctt gtagcgtccg
      901 cgcgtgatgg ccaccgacat cgtgacttgg ctggtcgccg ccaaggtttc gagaaactcc
      961 cgtattggag tccgttcacc tttcggaatg accagactta tatcaagctg gttggattcg
     1021 atcctgccgg tgatagtcgg cttcaccggc caattccgaa tcgcagcctc tactgcgatg
     1081 tcgctgcctt gaaccttgaa cgacaaccgc ttgagttcga cttcgttgcg cacaaatctt
     1141 acgcgtgcgt agagatcctg gatgggcccc tctatcccct tcgccaccat caacccattc
     1201 gtcaatccca tccatccgga gattcgccac gctttccaat ctgtgtctct ccctttgaca
     1261 tcaagtgaga gctcgaaatt ccctgcctca agccaggatt tggataccca ttccggcagg
     1321 ctggatactg acaatgtgct cgtcgccaca gccatatcga tcataaagcg atcaccgaag
     1381 tgtatggttc ccttggccg
//
LOCUS       DASTWE010001140         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_870808, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001140 DASTWE010000000
VERSION     DASTWE010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870808"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VFT30_11930"
     CDS             complement(<1..1575)
                     /locus_tag="VFT30_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5093389.1"
                     /translation="MTLEQGTGCVHIAPGHGMEDYLLVLEHNAKSSPGERLEILAPVD
                     NSGRFTAAVKEFAGQHVFKANPKIVDYLHANGRLLGHGSLSHSYPHCWRCKSPVIFRA
                     TEQWFVSMETNDLRKETLAEIERVRWIPSYGRERIFGMIQNRPDWCLSRQRVWGVPIP
                     GFTCHTCHHVLADPAVIEHVASLMESGGADIWFERSADELLPAGTTCPKCGGTHFEKE
                     RDILDVWFESGVSYAAVAKPRKWWPVDLYLEGSDQHRGWFHSALLAGVTTDRRAPYQA
                     VLTHGFVVDDHGKKMSKSAWNGVAPHEITKQHGAEILRLWVAAQNYQDDVRISTAIMT
                     QLIEAYRKIRNTCRFLLSNLYDFDPATHRVSYENLPELDRWALLRLGELIAKVRRSYD
                     DFEFHTIFHALNNFCSVDLSAVYLDILKDRLYTFRKDAHERRSSQTVLYDIIVAMAKL
                     MAPVLSFTAEEIWRTLAAQVSGGVGTASVHLASFPEFDPVWADAKLAERWERLLKYRT
                     LVQGVLEERRREKVIGS"
BASE COUNT          357 a          474 c          423 g          338 t
ORIGIN      
        1 ggagccaatg actttctcac ggcgtcgctc ctccaaaacc ccttgcacca gcgtacgata
       61 tttcagaagc cgctcccacc gctcggccag cttcgcatcg gcccagactg gatcgaactc
      121 gggaaacgag gcgagatgca cgctcgccgt tcccacgccc ccggatacct gcgcggccag
      181 cgtccgccaa atttcttccg ccgtgaaact caacactggg gccatgagtt tcgccatagc
      241 tacaatgatg tcgtacaaga cggtctggga gctgcgacgc tcgtgtgcat ccttgcggaa
      301 cgtatagagc cggtctttca gaatgtcgag gtagacagcg ctgagatcca ctgagcaaaa
      361 attattcagg gcatggaaga tcgtgtgaaa ctcaaagtcg tcataggatc gccgcacttt
      421 cgcgatcagt tccccgaggc gcaacaatgc ccatcggtcc agctctggaa ggttttcata
      481 ggagacgcga tgcgtcgccg gatcgaaatc gtacagatta ctcagcagga agcggcaggt
      541 gttacggatc ttccgatagg cttcgatgag ttgcgtcatg atggccgtgg agatgcgcac
      601 atcgtcctga taattctggg cggctaccca tagccgaagg atttccgccc cgtgctgctt
      661 ggtgatttca tggggtgcca caccgttcca agctgatttc gacatctttt tgccatgatc
      721 gtccacgaca aagccgtggg ttaataccgc ttggtagggg gctcgacgat ccgtcgtcac
      781 gccggccagc agcgcgctat ggaaccatcc acgatgttgg tcggaccctt caaggtacaa
      841 atcaaccggc caccatttcc tcggttttgc cacagcggca taactgaccc cggactcgaa
      901 ccagacatcg agaatgtccc gttctttctc aaagtgtgtt ccaccgcact ttggacaagt
      961 tgttccggca ggcaacagtt catcggccga ccgttcgaac cagatgtccg cgcccccgga
     1021 ctccatcaaa gacgcgacat gttcaatcac ggcaggatcg gcaaggacgt gatgacaggt
     1081 atgacaagta aacccaggaa tcggtacgcc ccacactcgc tggcgcgaga ggcaccagtc
     1141 cggccggttc tgaatcatgc cgaagatccg ttctcgtcca tagctcggaa tccatcggac
     1201 ccgttcgatc tccgccaacg tttccttccg cagatcgttg gtctccatcg acacgaacca
     1261 ttgctcagtc gcacggaata tcaccggact cttgcagcgc cagcaatggg ggtaggaatg
     1321 gctcagcgaa ccatgcccga gcaaacgtcc attggcatgg agatagtcga cgatttttgg
     1381 attcgccttg aacacgtgtt ggcccgcaaa ttccttcacg gccgccgtaa atcgtccact
     1441 gttgtcgacc ggagccagga tttcgagtcg ctcaccaggt gaagacttgg cgttgtgctc
     1501 aagcacgagg agatagtctt ccataccgtg ccctggcgca atgtgtacac agccggttcc
     1561 ttgctcgaga gtcacaaagt caccgagcag aa
//
LOCUS       DASTWE010001141         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_547956, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001141 DASTWE010000000
VERSION     DASTWE010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547956"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..>1188)
                     /locus_tag="VFT30_11935"
     CDS             complement(144..>1188)
                     /locus_tag="VFT30_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093390.1"
                     /translation="INGRHDEVKVFEGLLQEKLSRPCFFELIWSYWHEEGMLVQTMNA
                     ICMRFQNRRGPQERDPLANLEIDPLRPLNNLLWGYIQDEYNRLTIPRRAYEYDHHYGL
                     KLVGRAVPEVRSADSRSKFIEAFHNLLQRTAAFYREDADTTVIADGFSLLSSLREVHL
                     ILAEGAHNQFGDLPWTSRVEMLMMQWFLSRPEMREFLRGRAMVPYEETWMGQVDTMKR
                     LQGWTDTSVTHFRNLGTYGEQILLAIRYGDWSAENNQDAAKTWARYWRPEIQGYIHAY
                     LAATGVNLSEDVVDLRGAPTRYVQPSTLLQNRLAAQTSRGELPSGTRGSIPSGVNFAP
                     VTTRRRALNPRKE"
BASE COUNT          249 a          344 c          311 g          284 t
ORIGIN      
        1 aaagattcgg actcaattcg gctgctcatt ttgttacctc caccggaatc aactccggtc
       61 acacttctga cgtctgatct tcgtttctct gtcccagggt tgaaaccaac cctgggttgc
      121 aatcagccaa cgggatttag gtcttactct ttccgaggat ttagtgctcg acgtcgggtc
      181 gtcactggag caaagttgac tccactggga atcgagcctc gcgtacccga agggagctca
      241 ccgcgtgaag tttgtgcagc caatcggttc tgtaacaacg tcgacggctg tacgtaacgt
      301 gtcggcgcac cgcgcagatc gacgacgtct tctgacaggt ttacccctgt tgccgcgagg
      361 tacgcatgga tgtaaccctg aatttcgggt ctccagtaac gcgcccacgt cttggcagcg
      421 tcttgattgt tttccgcgct ccaatcgcca tatcgaatcg ccagcagaat ttgctcgccg
      481 taggtgccga gattgcggaa gtgtgtgacc gaagtatcgg tccaaccttg cagccgcttc
      541 attgtgtcca cctggcccat ccaggtctct tcatatggaa ccatcgcgcg gccacgcagg
      601 aactctcgca tctccggtct gctgaggaac cactgcatca tcagcatctc aactcgcgag
      661 gtccacggca gatcgccaaa ctggttatga gcgccctccg cgagaatcag gtgtacttcg
      721 cgcagtgagc tcaagagaga gaagccgtcg gcgattactg tcgtgtcggc atcttcgcgg
      781 tagaaagccg ccgtacgttg cagcaagttg tggaaggctt cgatgaattt cgaccggcta
      841 tcagccgagc gcacttcagg aactgccctg cccaccagct tcaggccgta gtgatgatcg
      901 tactcgtatg ctctgcgcgg aatggtcagg cgattgtatt catcctgaat gtaaccccac
      961 aacaagttgt tcaacggacg cagtggatcg atttccaggt tcgccagcgg atcgcgttcc
     1021 tgtggaccac gccggttctg gaagcgcatg cagatcgcgt tcattgtttg cacgagcatt
     1081 ccttcctcgt gccagtagga ccagatcagc tcgaagaaac acggccgact caatttctcc
     1141 tgcagcaatc cttcgaaaac tttgacctca tcatggcgac cgttgatg
//
LOCUS       DASTWE010001142         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_30902, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001142 DASTWE010000000
VERSION     DASTWE010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30902"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1594
                     /locus_tag="VFT30_11940"
     CDS             107..1594
                     /locus_tag="VFT30_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEU5093391.1"
                     /translation="MFNRIFGLETEYGLLIHEDRPNHSPTWFAHKIRDHLFQVQRRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYLDMGHLEYASPECHSLTDVVASDRAGDLLLQQA
                     LEELGFSEQVSLIKNNIDHETDATFGSHENYLVSRRFPFSRRGLVPFVTFLVTRQIFT
                     GSGRVGSAALQDAWIQTDRLLIPRSVLGAQHVSEPPFQISQRADYIVNDFFEWVQHNR
                     AIVNTRDEPLADPNQYRRIHLLLGDSNMAEYATALKLGTTGLVLQLIEEGHAPQDLEI
                     DEPVEALQEISQDQERRWLIQLRSQKTISALDIQERFLDCAVEHCAGQDEETDWVLTQ
                     WASVLHDLRGDYQKLVGRVDWASKLWLLECFRNAEHVDWIDPMMKSLDLEYHNLNHMK
                     GLYYGLLEEGRVPRITTDRLIHLAMETAPKNTRAYGRSELVRHLLNERPVSSAGQPPS
                     PDGLFPPYVINWSIFQVRGHAPFPMPDPFKTYLEDVRTHLESVIV"
BASE COUNT          490 a          569 c          588 g          555 t
ORIGIN      
        1 ctacttgatc gtcaggtgca aggaccatcc aagtatcgaa cactcgaggc cgatgagttg
       61 agaacagtac ttcctacgga tctgaaccca tcactcatct aatctcatgt tcaatcgtat
      121 ctttggcttg gagactgaat acgggctctt aattcacgaa gaccgcccca accattcccc
      181 gacatggttc gcccataaga ttcgtgacca tctattccag gttcagcggc gaggcgtgct
      241 ggacttgcat caccgtgggc atgatgagcc ccctggcaat ggaggatttc tcaccaacgc
      301 cggacgaatc tatcttgata tgggtcattt ggaatatgcc tcgcctgaat gccattccct
      361 tacggatgtg gtggcgtcgg accgtgccgg ggacctcttg ctgcagcaag ccctcgaaga
      421 gctcggcttc tcagaacaag tgtccttgat caagaacaac atcgatcatg aaaccgacgc
      481 gacattcggc tcgcatgaga attatttggt ttctcggcgg ttcccttttt caaggcgggg
      541 actggttcca ttcgtgactt ttcttgtgac caggcagatt tttaccggct ctggacgtgt
      601 cggttccgcc gccttacagg atgcatggat tcagacggat cggttactca ttccacgttc
      661 agtcctcggt gcgcaacacg tatcggagcc accctttcaa atctcccaac gtgccgacta
      721 tatcgtgaac gatttcttcg aatgggtaca gcacaatcgg gcgattgtga acacgaggga
      781 cgagccgttg gccgatccca atcaataccg acggattcac ctgctgctgg gtgattccaa
      841 catggccgaa tatgcgacgg cattgaagct cggaacgaca gggctggtgc tccagctcat
      901 tgaggaagga catgcgcctc aggatttgga gatcgatgaa ccggtggaag cacttcaaga
      961 gatttcccag gatcaagaac gacgttggct gattcaattg cggtctcaaa agacgatatc
     1021 ggctttggat attcaagaac ggtttcttga ctgtgcggtt gagcattgtg cagggcagga
     1081 tgaagaaacc gattgggtcc ttacccaatg ggcgtcggtg ctccatgacc tgcgcggtga
     1141 ctaccaaaag ctcgttgggc gtgtcgattg ggcatccaag ttgtggctgc tggaatgctt
     1201 tcgaaacgcg gaacatgttg attggatcga tcccatgatg aaaagtctgg atttggagta
     1261 tcacaatctg aatcacatga aggggctgta ttacggatta ttggaagagg gacgtgtccc
     1321 tcgtataacg acagaccgcc ttatccatct tgcgatggaa acggcgccga aaaacacacg
     1381 tgcctacggc cgcagtgaac tggtcagaca tctcctgaat gagcgacccg tctcatcggc
     1441 gggacagccg ccatcccctg atggattgtt ccctccctat gtcattaatt ggtcgatctt
     1501 ccaagtgcgt ggtcatgctc cgtttcccat gccggaccct tttaagacat atcttgaaga
     1561 tgttcggaca cacctcgaaa gcgtgatcgt ctaagctgct cagattcagg aatggcagtt
     1621 tgtttcttcg tcagaatcct ctcgtgctaa agctttagtt cgtaccagag gtgggttatg
     1681 gtagccagga agtggggatg gtatgtggcg ttgccttggt ctgacagttc ttcttgccat
     1741 gattcttggg caggtttcat tgtttcgaga aactcggtag gaaaattcct ggcctcagac
     1801 cttctccaat tcgtgggtta cagcggttca ttgatgattg ggtggtcggg cgctcgagag
     1861 ttggcgagaa atcctggtca tgccctcgcg acgccgctcg caatcactag tgcatcgtgc
     1921 tgcggccgtt gttgccgaca gcatctgtct cattctcagc tgggtatggt agtacgctcc
     1981 tctcgtcgcc gcatggagtc tcgcaagctt cgcaaagctg cgtagctgcc cgctctggga
     2041 caaggttgac gcagcatcgt catagacgtc ctagcaaaaa gtcaatgatt cggttctccg
     2101 cccagtacgg atcttctcca ttccgactcc ggtgaaaaaa cccgcaatgg cctccatagc
     2161 gaggcgcgac cacacgaatc tgaggatgcc gctgaatctt tg
//
LOCUS       DASTWE010001143         1167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1097230, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001143 DASTWE010000000
VERSION     DASTWE010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097230"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..1035
                     /locus_tag="VFT30_11945"
                     /pseudo
     CDS             565..1035
                     /locus_tag="VFT30_11945"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
BASE COUNT          300 a          283 c          311 g          273 t
ORIGIN      
        1 gagaaagtct gtagcgggaa agctttgtcc gccgactctc tggcattaga atgcacacgc
       61 tatcgataga taggtccggc ggtacccaaa caggctatga aagaggacta ggggtttccc
      121 cagtcgatct ttacgcggtt cttttttata aaaacgctcg cttcggcagc ggaacaacgc
      181 cgttcccaat acaaaccgaa ttccctaggg acaacgccgt cctttaaggc taaagcaggc
      241 cggaaggacg cttgtctgtc tgcagcgttc ctccagtcga aatcgcctag aatcaagcat
      301 tttgcgtctc gtggagttgc cgccatcgtt atttaatcat tattgatact aacttaagga
      361 ggaagagatg gacagccaaa cgtcagtgat tcgcaatgaa gtatttgtag ccactacctg
      421 ccctggggag catcgtctct ggaagcacat gccacgcata tctccctgtg atggtgtcgt
      481 gaccggtcca ttgccagacc aaatctggat tgcaatggag tgggcacgtc ttggtgggga
      541 ttaaggtaag ggttcgaatt cacaatggaa gcgcttagac cgccgctgcc accgttcgac
      601 cggtcgacgg cggcccagaa agtccgtatt gcggaagatg cgtggaacac tcgtgaccca
      661 aaactcgtcg cattaggtta tacgcaagat agcgtctggc ggaatcgttc cgaatttctt
      721 gcgggtcgcg aagcgatcgt ccaattcttg acccgaaagt ggaataaaga actagactac
      781 cggctcacca aggaactctg gacatttcac gaaaatcgga tcgctgtgcg gttctgttat
      841 gagtggcacg atgattcggg caattgattt cgctcctatg gcaatgagaa ttgggagttt
      901 gaagataacg ggtatatgcg tcgacggatt gcaagcatca atgaccttcc cattgaggac
      961 aatcagcgaa aattccactg gccgcttggg cgtcgacctg atggccatcc aggactgtca
     1021 gagctaagtc tgtgacttag cgcaaaacgg cttagaagtt ttggagaata tccgaaaagc
     1081 atacgagacc accggtcata gtcgaagcgc ctcgcgtatc tgcgcatgat catcgaccag
     1141 gagaattcga catgtgtgca actgcat
//
LOCUS       DASTWE010001144         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1355716, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001144 DASTWE010000000
VERSION     DASTWE010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355716"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..>1110
                     /locus_tag="VFT30_11950"
     CDS             43..>1110
                     /locus_tag="VFT30_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5093392.1"
                     /translation="MAVTADHARHEIPQDTPIIIVIPGFGSTQPDYVPLSFYLAANHL
                     RVLRYDHSNHVGQSEGNILQFTLRNMQVDLQIVLDFAHTTWPTAPVALLAEDIAARVA
                     IKVMARNTSPDRLFLLNPVFDIETALSTIDRPNVIETYRQGHRRGVANLWGLNVNFDK
                     FVGDAISGEYVDLASSRTDFAQLAIPPVILVSPRKNRPIEHTFGPQYHSLTALGTVPI
                     VVSLPADVSGESGAKDERRTAALKTSLKLISTSLLGDPSSAQVREPNLREMYQQRQLE
                     HERIRIRYHVSQATRSALWGAHLAHLPQLENLPAYLALTNELYRRFLPLEPGMTILDI
                     GCGQRNFVRHLLTNQAYKSAHQ"
BASE COUNT          246 a          355 c          260 g          249 t
ORIGIN      
        1 aaaagccaca gatgggaatg tcggaatacg catggccagg ctattgcggt gacggccgat
       61 catgcccgcc atgaaattcc acaggacacc ccgataatca tcgtcattcc aggattcgga
      121 tcgacccaac ccgactatgt accactctcg ttttacctcg ccgccaacca tttacgcgtg
      181 ctgcgctatg atcactcgaa ccatgtcggg caaagcgagg ggaacattct gcagttcaca
      241 ttgcgtaaca tgcaggttga tctccagatc gtcctggact ttgcccatac cacatggccc
      301 accgctccgg tggccctcct cgccgaagac attgctgcga gagtggcgat aaaagtcatg
      361 gcccggaaca catcgcctga tcgacttttc ttgttgaatc ctgtcttcga catagaaacc
      421 gctctttcca ccattgatcg ccccaacgtc attgaaacct accggcaagg ccatcggcgg
      481 ggcgttgcca atctctgggg ccttaatgtc aactttgaca agtttgtcgg cgatgcgatc
      541 tctggagaat acgtcgactt ggcctcgtca agaactgact tcgctcaact ggccattccg
      601 cctgttatcc tggtctctcc cagaaagaac cgtcccattg aacacacgtt cggccctcaa
      661 tatcattccc tcaccgcttt gggaaccgtc ccaatcgttg tatccctgcc ggccgacgtg
      721 tccggagagt ccggcgccaa ggacgagcgc cgtaccgccg ccctcaagac gagcttgaaa
      781 ttgatctcaa cttccctgct cggcgatcct tcctcggctc aggtgcggga accgaacctc
      841 cgtgaaatgt accaacaacg gcagttggag catgaacgca tccgcattcg ctaccacgtc
      901 tcgcaggcca cacgcagcgc cttgtggggt gctcatttgg cccatctgcc ccaacttgaa
      961 aacttgccgg cctatttggc cttgacgaat gaactgtatc gccggttcct tccccttgaa
     1021 ccaggcatga ctatcctcga tatcggctgc ggtcaaagaa acttcgtccg tcacctgctg
     1081 accaatcagg cctacaaatc ggcgcatcaa
//
LOCUS       DASTWE010001145         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_127728, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001145 DASTWE010000000
VERSION     DASTWE010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127728"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VFT30_11955"
     CDS             <1..1052
                     /locus_tag="VFT30_11955"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU5093393.1"
                     /translation="ITGRHEVAWQIIQTFSSYVSEGMVPNRFPDMSEDPEYNSIDASL
                     WFVHAVERYLTYSEDATRVRSIAWPAIKQILDGYRQGTRYDIHMDVDGLIVGGTPGIQ
                     LTWMDVKIGDWVVTPRHGKPVEIQALWLRALEVGARLAVQFGEVDYATRCQEDRAQGV
                     ESFRVKFWYKDGQYLYDTINGPEGDDLSIRPNQVFAIVLCDDLLTREQAKQVLQIVKD
                     HLLTPVGLRTLSPTDVRYRPRYEGGPIERDSAYHQGTVWPFLLGPFITAWLKTFGQTG
                     KTRTEARSFLDGIEAHLQEACLGQVSEIFDGDPPHHPRGCTAQAWSVAEPLRALVEDL
                     GVPITTGVARSSRRK"
BASE COUNT          274 a          291 c          334 g          285 t
ORIGIN      
        1 tcattaccgg acggcatgag gtcgcctggc aaatcattca aactttttcc tcctatgttt
       61 cggaaggcat ggtgcccaat cgatttccgg acatgagcga agacccggaa tacaactcta
      121 tcgatgcgtc actgtggttc gtccatgccg tcgagcgata cctcacgtac agcgaagatg
      181 caacacgtgt gcgatcgatc gcttggccgg cgataaagca gattctcgac ggctatcgcc
      241 aaggcacgcg gtacgacatc catatggacg tcgatggcct cattgttgga ggcacgcccg
      301 gtatccaact tacctggatg gatgtgaaaa tcggcgattg ggttgtcaca ccacggcatg
      361 ggaagccggt agagattcag gctttgtggc ttagggccct ggaggtcggc gcacgacttg
      421 ctgtccaatt tggcgaagtc gattatgcca cgcgctgtca ggaagatagg gcacaagggg
      481 tagagtcttt ccgcgtgaaa ttttggtaca aagacggaca gtatttgtac gacacgatca
      541 atgggccgga aggcgacgac ctatctattc gtcccaatca agtttttgcg atcgtcctgt
      601 gtgatgatct gctgacgaga gaacaggcaa aacaggtgct ccaaatcgtc aaggatcatc
      661 tcctcacgcc ggtgggactg agaacgttgt cgccgacgga cgttcgatat cgtcctcggt
      721 acgaaggcgg tcctatagaa cgagatagtg catatcatca gggaactgtc tggccgtttc
      781 tcctgggtcc ctttatcaca gcctggttga aaacgtttgg acagacgggc aaaacacgaa
      841 ctgaggctcg gtcctttctt gatgggatag aagcacattt acaggaagct tgtctcgggc
      901 aggtgtcgga aatttttgat ggggatcctc cacatcatcc gcgaggttgt acagctcaag
      961 cttggtccgt cgcggagcct ctccgtgcat tggttgaaga cctcggggta ccgattacga
     1021 ccggagtagc ccgctcatct cgccgaaaat aagtgctcaa accgaaattg atcgattctt
     1081 cctctcttga cagggtttgg aggcatccgt agtctgattg ctgggtacgt gtgggagggt
     1141 taagaacgat gtcatttttc attccgaacc acaatagccg gctg
//
LOCUS       DASTWE010001146         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1323305, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001146 DASTWE010000000
VERSION     DASTWE010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323305"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFT30_11960"
     CDS             complement(<1..432)
                     /locus_tag="VFT30_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093394.1"
                     /translation="MALRVLPSLSLAVTEETVRKQKRIVMFVPGLIAFGVYRLVKHLV
                     PIADPLVLLTVSGLVATVTSVLAYRVGRSAPWSVIVRQDGARLLSWLAGWIGAVYGIQ
                     LSLLVLTLLWIMKYDYLQHPDGPAMMAIIISCTAVARDAFEI"
     gene            complement(539..1003)
                     /locus_tag="VFT30_11965"
     CDS             complement(539..1003)
                     /locus_tag="VFT30_11965"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HEU5093395.1"
                     /translation="MHTEGIEERAGESGGPQWFIRSECRGCGLKVGIDVPEGQMHGLV
                     DRLLWTDEALHRLDRLPPYVATLVQKDAEQDARQRGFPVITYDALLRPKIGERIEWEP
                     EAERRLERVPAPVRAMARIELERTAADRGLSRITVSLMEEVKAKYFGMGASK"
     gene            complement(1040..1753)
                     /locus_tag="VFT30_11970"
     CDS             complement(1040..1753)
                     /locus_tag="VFT30_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEU5093396.1"
                     /translation="MDWLREPGFFGTHATAGADLSQLMATLFTTLFVVGWFQARKRQA
                     DTHHWLMLGGMISMLSFFIAYYLFRQLGVLAVEGKEGFGGSQSLYDYVFIPVLTLHII
                     LVIIGLIMAVYMIVLGFRAQQVILGVRSLRESRLLTTWKKIGVIFGGIAVVVLGLFFS
                     RVATAGFSMRKLEVYLVFLIVVAFVFAIEMTIQRIWPDGAQRHRALGRFTMVIYCVLF
                     VTGSFTYTMLYILYPGKIG"
     gene            complement(1817..>2394)
                     /locus_tag="VFT30_11975"
     CDS             complement(1817..>2394)
                     /locus_tag="VFT30_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU5093397.1"
                     /translation="VLNRIGQQSGPDFAKRLELMGSERLLDLGGGAGTNAIAFCQVYS
                     ELRATVFDLPATLKLTEKTVKDAGLESRITLHPGDFNRDQLGGPYDVVLMSDILHYQT
                     FQMNHDLVKKVFVSLAPGGRLIIKDRFLDEAGTGPAWTTAFTIHILVNTQYGSCYKCS
                     DAMQWLTESGFDSVVELEKTAVVQGLKSLEA"
BASE COUNT          626 a          711 c          557 g          500 t
ORIGIN      
        1 gatttcaaat gcatcgcgag cgaccgcggt acaagagatg atgatcgcca tcatggcagg
       61 cccatcggga tgttggagat aatcgtactt catgatccac aacagggtca gcaccaacaa
      121 cgagagctga atgccataga cggcaccgat ccagccggcg agccagctga ggagacgcgc
      181 tccatcttgg cggacaatga cggaccatgg ggcggaccga ccaacacgat aggccaggac
      241 cgaagtgacc gttgccacca gaccactgac cgtcaacagt acaagaggat ctgcgatcgg
      301 aacgagatgc ttgacgagac gatatacccc gaacgcgatc aatccgggga caaacatcac
      361 gatccgtttc tgcttgcgca cggtctcttc cgtgaccgcc aaacttaaac tcggaagaac
      421 ccgcaatgcc atccggtgca acatgatatt caggactgag gcagcaggac tgcggactga
      481 gctgagggaa tggccggttt cattcttttg tccattctca gcccctcagt cctcattact
      541 atttagaggc gcccattcca aagtacttcg ctttcacttc ttccatcaga gacacagtaa
      601 tacgagacaa tcctcgatcc gcagccgtgc gctcaagttc gattcgagcc atggcacgaa
      661 caggagccgg tactctttcc aaccgccgtt ccgcctcagg ctcccattca attcgctcac
      721 cgatttttgg acgcaacaac gcatcgtacg taatcaccgg aaacccacgc tgacgagcat
      781 cttgctccgc atccttctga acgagcgttg ccacataggg gggaagacga tccagccgat
      841 gcaatgcctc atcagtccac agcaatcgat cgacaagtcc gtgcatctgc ccctccggta
      901 cgtcgatacc gacctttagt ccacaccctc ggcattccga tcggatgaac cattgcgggc
      961 cgccactttc gccggcacgt tcttcaatcc cttctgtatg catccatcga ccacagccgc
     1021 aggtcagcat ctcgataggc tacccaatct tcccgggata aaggatatac agcatggtgt
     1081 aggtaaagct ccccgtgaca aacaagacgc agtaaatgac catcgtaaat cggccgagcg
     1141 cgcgatgtcg ctgtgcgcca tcaggccaga tccgttgaat cgtcatctcg attgcgaaca
     1201 caaatgcgac cactataaga aatacgagat acacctccag ctttcgcatt gaaaaaccgg
     1261 cggtcgccac acgcgaaaaa aataacccga gcaccaccac ggcaattcca ccgaaaatga
     1321 ccccgatctt cttccacgtc gtgagcaacc gtgattcccg aagggaccgt actccgagaa
     1381 taacctgttg ggcccgaaag cccaacacga tcatgtacac cgccatgatc aagccgatga
     1441 tgacgagaat gatatggagc gtgagcaccg gaataaagac gtaatcatag agcgattgcg
     1501 agccaccgaa tccctctttt ccttccacgg ccaatacgcc aagctgtcgg aagagatagt
     1561 aggcaataaa gaaactgagc atcgaaatca ttccgcccag catcaaccaa tgatgtgtgt
     1621 ccgcctgtcg tttgcgagcc tgaaaccatc caacgacaaa gagagtggta aacagcgtgg
     1681 ccattaactg gcttaagtca gccccggctg tggcatgggt tccaaaaaat ccaggctctc
     1741 ttaaccaatc catcgcgctg ctcttcctcg ctcgtatctt gaggagcgtc cctcaaaatt
     1801 gggcgcgttt caccgactac gcttcaagag atttcaagcc ttgcacaaca gccgtctttt
     1861 ccaattctac gacagagtca aatcccgact cggtcaacca ttgcatcgca tcgctgcatt
     1921 tgtaacaact gccgtactgt gtattcacga gaatatggat cgtgaacgcg gtggtccaag
     1981 ccgggccagt accggcctcg tccaggaatc gatccttaat gatcaatcgc cctcccggcg
     2041 ccaacgatac gaagactttt ttgaccagat cgtgattcat ctggaacgtt tgatagtgaa
     2101 gaatgtccga catcaaaacc acatcgtatg gacccccaag ttgatctcta ttgaaatcac
     2161 caggatgcag tgtgattcgt gattccaacc ctgcgtcctt gaccgttttc tccgtcaact
     2221 tcaacgtagc cgggagatca aagactgtcg cacgtaattc ggagtacacc tggcaaaagg
     2281 cgatggcgtt ggttcctgcg cctccgccca ggtccaacaa ccgttccgat cccattagtt
     2341 ccaatcgctt ggcaaaatca ggtccgcttt gttgaccgat acgattaagg accg
//
LOCUS       DASTWE010001147         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_385932, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001147 DASTWE010000000
VERSION     DASTWE010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385932"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..>1044)
                     /locus_tag="VFT30_11980"
     CDS             complement(67..>1044)
                     /locus_tag="VFT30_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5093398.1"
                     /translation="AITNAHHLLTHYPDPDCLELRQTLVRLWERPPEHIAIGNGSTEL
                     IDVLPRALKIRHLLVVQPTFSEYAASMARSGGRVSAVYAKRTERYAQPIEELCRLIEK
                     HQHGMDAFNGVMLCNPNSPSGQACSIDDVQRLAQAVQRRGAWLVIDEAFADYCPERSF
                     VPQAASWPHVVMLRSLTKFYALPGLRIGYAVAKPSVIELLRRHMPPWSVNAIGQVAAL
                     AAVGDSAHARKSIQFMAKERDRFGKLLAALPGCSVMPTHANYFFMELPHGWHAREVTE
                     QLRNEGLLIRDCSSVPGCNARSIRLAVRSQQDNDQFIQSLSRLLHRPVS"
BASE COUNT          245 a          280 c          277 g          242 t
ORIGIN      
        1 gattgatcac gagcgtgcgt ccaatcactt gatgccgagt gcagacgcgt gaccggattt
       61 tctttttcat gacacagggc ggtgaaggag tctggataag gactggatga actgatcgtt
      121 gtcctgctgg gatcgcaccg caagtcggat agaacgagcg ttgcaccctg gaacggatga
      181 acaatcgcga atcaacagtc cttcatttcg caactgctct gtcacttcac gcgcgtgcca
      241 tccatgaggc aactccataa agaaataatt ggcgtgcgtc ggcatcactg aacagccggg
      301 caaggcagca agcagtttcc caaaccggtc tcgttctttt gccataaact gtatgctctt
      361 tcgagcatgg gccgaatctc ccacggcggc aagcgcagcc acttgcccta tggcattcac
      421 tgaccagggc ggcatgtgcc tccgtaacaa ttcgatcacg gagggctttg caactgcata
      481 tccaatccgc agaccgggta atgcatagaa tttggttaag ctacgcaaca tcacgacatg
      541 cggccacgag gcagcttggg gtacgaatga ccgttcaggg cagtagtcgg caaacgcttc
      601 atcgatgacc aaccaagcgc cacgtcgttg aacagcttgt gccaatcgtt gaacgtcatc
      661 gatggaacat gcttgcccgc ttggactatt gggattgcag agcatgactc cgttgaatgc
      721 atccattcca tgctggtgtt tttctatcag acgacacagc tcttcaatcg gttgagcata
      781 tcgttccgtg cgcttagcat agacggcgct gacgcgtcct ccgcttcgtg ccatcgatgc
      841 tgcatattcc gagaaggtgg gttgcaccac caggagatgc cgaatcttga gagcccgagg
      901 aagcacatcg atcaattctg tcgaaccgtt tcctatggca atgtgctccg gaggtctttc
      961 ccacaacctg acgagcgtct gccgtagctc caaacaatcc gggtctggat aatgagtcaa
     1021 tagatggtga gcgttggtga tcgc
//
LOCUS       DASTWE010001148         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_580552, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001148 DASTWE010000000
VERSION     DASTWE010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580552"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="VFT30_11985"
     CDS             complement(<1..1882)
                     /locus_tag="VFT30_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5093399.1"
                     /translation="MKTYNKHRSIGGCGLAVVAGLALALGCFLSSDGYTAQVETSEQP
                     TTNGCTTAEGCFAAAAWPKERLGHALTKDQVGALKLERLRKVIEEFPATLWAKRAGLL
                     SGVILIDRNPAAALPHLRTAQRDFLVLDDYIRFWIAEALLRLGDAKEAAGMFEGVPQA
                     IPDSNLLNQVALRAGEAWYQASSCPEAVSWFAKAVDVNDKDPQISQVWLRLASCYIRG
                     NQLTEGRETLKQLWTRFPHTKEAKEAETLLRGNIGGEPWTTLPDMHYERAQAFMGQSL
                     HAEAIEELKKFLAQDTSSPHRGDAKLKLGIAQVRLKLYDQARDTFHALTAEQGPRADE
                     ASVWLGRVYLRQGLGEKLLDLCRTLPKRTVTPEQKGQINLFAGIWLEDEARFDEAIAR
                     YRQVAKFGEPASQRAEAQWREGWVLYRTARYREAINVWRQIVDQKDNDFEPQALYWIA
                     RSYGHVEDVKSKEAFALLCQRFPHTYYCQLAREHTDLSISGQTKREGSVVAASSTQPA
                     SEMTQMSVQDNHTSSRMQIELQPAYRRAVELRTLGLEQDAARELGALTDRYSRDPEML
                     AALSMMLNEVGAYHHALRLVRSRFREKLERTGGVVADGLWSVAYPTGLIPTIKMSGAN
                     GVD"
     gene            2037..3122
                     /gene="rlmN"
                     /locus_tag="VFT30_11990"
     CDS             2037..3122
                     /gene="rlmN"
                     /locus_tag="VFT30_11990"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5093400.1"
                     /translation="MTLSGTVTTLLGLTEPQMLKFVRTHRWPNYRATQILRWIYQRRL
                     RTITDMTDLSMHDRMMLSQSATIGRSAHVRVLPSRDGTRKLLLTLDDGMSIEAVLIPD
                     EERLTLCVSTQVGCTLDCGFCLTGQMGLKRNLKLHEIIDQILTAQDLLGSDEHITNLV
                     FMGMGEPLANLDGLKAAVTVLTNKSWGLGWSRRRVTVSTAGLASRLPEIAALGVNLAI
                     SLNATTEEQRRALMPAASEIASLRSLLAACRSYPLPSHQRLTFEYVLLAGVNDNAADA
                     RRLVQLLRGMRCKANLIPFNEFPGSPFKRPTEQAVLRFQSILRDAGLDAFVRKSRGRD
                     VLGACGQLGELSGNNPSLTLTPIETRC"
     gene            3147..4520
                     /locus_tag="VFT30_11995"
     CDS             3147..4520
                     /locus_tag="VFT30_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5093401.1"
                     /translation="MRTIQHHWRIHFLAALLAFGSTSISLAAEPSEYILSNGMKVLLV
                     EVPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTARYPKGSFSRIVRKNGGI
                     DNAFTGQDFTAYFENLAADRVGLALELEADRMQGLILDHSEFQTERDVVKEERRLRSE
                     DDPQGALVEALFAHAFLSHPYHWPVIGWFADLDAMSLEDLQRHYDTFYSPNNATLVVV
                     GDIKAESLLPTIKRLFEPIPRGPSPKQILSPEPEQRGERRFLLKREAQVPFVMMGFRV
                     PNYSNEDSYALDILESILSSGKSSRLYQSLVYDQKNSLAVGAEYSVLQTDPGLFYFYS
                     LVNPSVKVEGVEEAIQREIVRLQNEPPSEQELQRAKNQVEAARVFEQDSNFRHAMLMG
                     QAESVGAGWRRIDQFVERIRAVTAKDIQRVAKQYLTPDNRTVGILIPLPSPPPDSAPT
                     AAHEGKS"
BASE COUNT          995 a         1377 c         1173 g         1016 t
ORIGIN      
        1 gatcaactcc attggctccc gacattttaa tcgtgggaat caatccagtt ggataggcca
       61 cgctccaaag tccatctgcg accactcccc cggttcgctc cagcttctca cggaatcgag
      121 accgcacgag gcgcaaggca tgatggtagg caccgacctc attcaacatc atcgataacg
      181 ccgccaacat ctccgggtca cgactatacc gatctgtcaa cgcgccaagc tctctcgcgg
      241 catcctgctc gaggccgagt gttctgagtt ccaccgcccg ccggtatgcc ggctgtagct
      301 cgatctgcat ccggctgctt gtgtgattgt cctgaactga catctgagtc atctcggagg
      361 caggctgagt ggacgaagca gcgacgacgg aaccttcccg tttcgtctgc ccggaaatcg
      421 acaggtccgt gtgttcgcgc gccaattggc agtagtaggt gtgcggaaat cgctgacaga
      481 gcagtgcaaa cgcctctttt gacttcacat cttccacatg gccgtaagaa cgggcaatcc
      541 agtagagcgc ctggggttca aagtcgttgt ccttctggtc gacgatctgc cgccacacgt
      601 tgatcgcctc tcgataacgg gccgttcggt agagcaccca cccttcccgc cattgtgctt
      661 ccgctcgctg agaagccggt tctccgaatt tggccacctg tcgatacctt gcgatcgcct
      721 catcgaaccg tgcctcatct tccagccaga tcccggcaaa taaattgatc tgccctttct
      781 gttccggagt cactgttcgc ttcggaagtg tccggcagag atccaataac ttctccccca
      841 gaccctgcct gagatacact cttcccagcc atacactcgc ttcatccgcc cgaggtcctt
      901 gctcagcggt cagtgcatga aaggtgtcgc gggcttgatc gtaaagtttc aaccgaactt
      961 gggcgattcc caatttgagt ttggcatctc cgcggtgagg agaagacgtg tcttgcgcca
     1021 agaacttttt caactcctcg atcgcctctg catggagcga ctgtcccatg aaagcctggg
     1081 cccgctcata atgcatgtca ggcaacgtcg tccacggctc ccccccgata ttgcctctaa
     1141 ggagagtctc agcttccttg gcttcttttg tgtgtggaaa cctcgtccaa agctgcttca
     1201 acgtttctcg cccttccgtc aattgattcc ctcgaatata acaagacgcc aaccgcaacc
     1261 atacctgaga gatctgcgga tccttatcat tgacatcgac ggccttggcg aaccaggaca
     1321 cagcctcggg acagctggac gcctgatacc acgcttcccc ggcacggagc gccacctgat
     1381 tgagaagatt ggaatcgggg atcgcttgcg gcactccctc aaacatgccg gccgcctcct
     1441 tggcatcccc taaacgcagc aaagcctctg caatccagaa tcgaatataa tcgtcgagca
     1501 cgagaaagtc ccgctgggct gttcgaagat gtggcagagc agcggccggg tttcgatcga
     1561 tcaagatcac cccggacagt aacccggcac gtttggccca cagcgtagcg ggaaattcct
     1621 ctattacctt tcgaaggcgt tccagcttga gcgcccccac ttgatctttc gtcaaagcgt
     1681 gccccagacg ctcctttggc caggccgccg ccgcaaaaca accctccgca gtcgtacaac
     1741 cattggtcgt cggttgttcg gacgtctcaa cttgcgccgt atatccgtcg cttgaaagaa
     1801 aacatccgag cgcgagcgcc agacccgcga caaccgccag cccgcaacca ccgatggaac
     1861 gatgcttgtt ataggttttc atgacaacct acctaccgtt actcgtccgc gtttattata
     1921 tacggagtgg tccgcaatgg aagaagtttc aggacccgat gacggacata gccgtatgcg
     1981 gcatgagttc cgctatggta ggctcctagc ttgaggatga tattgtcgac ttgccaatga
     2041 ccctttccgg caccgtgaca acgctcttgg gtctcaccga accacagatg ttgaagtttg
     2101 tccgcacgca ccgctggccg aactatcgtg caacgcaaat cttgcgctgg atctatcaac
     2161 gacggcttcg aactatcacc gatatgaccg acctctctat gcacgatcgc atgatgctgt
     2221 ctcaatcggc cacgatcgga cgctcggcac atgtcagggt cttgccgtcc cgggacggaa
     2281 cgcgcaaatt actgttgact ctcgacgacg gcatgtcgat cgaagccgta ctgatcccgg
     2341 atgaggaacg gctgacgctc tgcgtctcga ctcaggtcgg ttgcacattg gattgcggtt
     2401 tctgtctgac cgggcaaatg gggcttaaac gcaacctcaa gcttcacgaa atcatcgatc
     2461 agatcctaac cgcgcaagac ttgctgggtt ccgatgagca cattacgaat ctcgtgttca
     2521 tggggatggg agaacccctg gccaatttag acggtctcaa ggctgccgtg accgtcttga
     2581 ccaacaaatc gtggggtctt gggtggtcgc gtcgacgcgt gacggtatcg acggcggggc
     2641 tagcatcccg tctcccggaa attgcggcgc ttggcgtgaa cctcgcgatc tcgctcaatg
     2701 ccaccactga agaacaacgg cgagcgctga tgcctgccgc cagcgaaatc gcttcgctga
     2761 gatcactcct ggctgcctgt cgcagctacc cgcttccctc tcatcaacgg ctgaccttcg
     2821 aatatgtatt gctcgccggc gtaaacgaca atgcggctga tgcccgccga ctagtacaat
     2881 tgttgagagg catgcggtgt aaggctaatt tgattccgtt caatgaattt ccaggcagcc
     2941 cttttaagcg tccgaccgaa caagcggtgc ttcgttttca atccattctc cgcgacgccg
     3001 gcctcgatgc ctttgttcga aaaagccgag gacgagacgt tctcggcgcc tgcggacaac
     3061 ttggagagct ctccggtaac aacccctccc ttaccttgac acctatcgaa actcgttgct
     3121 agcatgacga gttccatctt catcatatgc gaactatcca gcatcattgg cgcatccatt
     3181 tcttagcggc gcttctcgcc ttcggctcca cttcgatttc gctcgcagca gaaccgagtg
     3241 agtacattct ttcaaacggt atgaaggtgc tgctggtcga ggttcccaag gctccggtgg
     3301 ccacggtgca ggtatggtac aaagtcggtt cgcgaaacga ggtcatggga cgggccggac
     3361 tctcacacat gcttgagcac atgatgttca aaggcacggc aaggtatccg aaaggctcgt
     3421 tttcccgaat cgtcagaaag aacggcggaa tagacaacgc gtttactgga caagacttca
     3481 ccgcctattt tgagaatctc gcagccgatc gtgtcggact ggccctcgaa ctggaggccg
     3541 atcggatgca gggcctcatt ctcgatcaca gcgaatttca gaccgaacgc gacgtcgtga
     3601 aagaagaacg ccgcctcaga tccgaggatg acccccaagg tgcgctggtt gaagcgctgt
     3661 ttgcccatgc cttcctcagt catccctacc attggcccgt catcggatgg ttcgccgatc
     3721 tcgatgcgat gtctctcgaa gacttgcaac gccactacga cacgttttac tctcccaaca
     3781 atgcgaccct tgtagtcgtg ggcgatatca aggccgaatc actacttccg acgatcaaac
     3841 gtctgtttga gccgatcccc agaggcccct cacccaagca aatcctgtcg cccgaaccgg
     3901 aacagcgagg cgaacgccgt tttctcctga agcgcgaagc gcaagtgccg tttgtcatga
     3961 tggggtttcg cgtacccaat tattcgaacg aggactcgta tgctctcgac atcctcgaat
     4021 cgatcctgtc cagcgggaaa agctcccgcc tctaccaaag cttggtctat gaccaaaaga
     4081 attcgttggc cgtcggtgcc gagtacagtg tgctgcaaac ggatcccggc cttttttatt
     4141 tctactcatt ggtaaatccc agcgtgaagg tcgaaggggt cgaagaagcc attcagcgtg
     4201 agatcgtccg gcttcagaat gagccgccgt ccgagcaaga gcttcagcgg gccaagaatc
     4261 aagttgaggc ggcgcgcgtc ttcgagcaag attcgaattt ccgccatgcc atgttgatgg
     4321 ggcaggctga atccgtcggc gccggctggc ggcgaatcga tcaattcgtg gaacggatcc
     4381 gtgcggtcac ggcgaaggac attcagcgtg tggcgaagca atatcttacc ccggacaatc
     4441 ggaccgtcgg aatactcatt cctttgcctt cccctccccc agactcagcc cccactgccg
     4501 cccatgaggg gaaatcataa aagaatcatg atgaccgagc cgaaagacca gaggcgtcat
     4561 t
//
LOCUS       DASTWE010001149         1198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_774622, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001149 DASTWE010000000
VERSION     DASTWE010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774622"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFT30_12000"
     CDS             complement(<1..1091)
                     /locus_tag="VFT30_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093402.1"
                     /translation="MRSDPLAEFRRVVSFRARQFPRQWEASRKLLEGTTFPSTIVRLC
                     DAVQSKDLPASVKETLLRLFERYAARRVQDLDGEGLKSVTGLPPAKALRALAVFFELV
                     PPTAAAKWPVTYLSSEEVEEAVRDLENPFDLLRHIDVASVLDIGAGDLSFAEELVDLY
                     GSELKQQQQPFIVHCLDRLDPRSQLGGPLHANPERLQKLQRNEGLSFSFFGNQDMFEL
                     GDLDQQELLAPRYAIATCWAPATPTFAYEPTRLSEVLIRNELERTKGAFHQTRLGKEQ
                     ALEVRHAGRALLFPPWKFEIVGPLALLSLLACRGFLCVLGSVDAQVFWELLAQLLEDS
                     RYRPPDQPFDPTNLPKVFGEVYHALDGLP"
BASE COUNT          286 a          320 c          345 g          247 t
ORIGIN      
        1 atcggcagac cgtcaagagc atgatagacc tcgccaaaga ccttgggcag attggtgggg
       61 tcaaagggtt ggtccggcgg gcgatatcgc gaatcttcca gcaactgtgc caggagttcc
      121 caaaacactt gtgcatctac cgatcccagg acacataaga atccgcgaca agcgagcagg
      181 ctcaggaggg caaggggtcc gacgatctca aacttccatg gcgggaagag gagcgctcgt
      241 ccggcatgcc tgacttcgag tgcttgttct ttccccaaac gtgtctggtg aaatgcacct
      301 ttggtacgct ccagctcgtt gcggatgagg acctcggaga gccttgtcgg ctcataggca
      361 aaggtcggtg tggccggtgc ccaacacgtg gcgatggcat atcgtggggc cagcaattcc
      421 tgctgatcca gatctcccag ctcaaacatg tcttgattgc caaagaaaga gaaggatagc
      481 ccctcattac gctgcaactt ctgcagcctc tccggattcg cgtggagcgg accgcccagt
      541 tgagaacggg gatcaagtcg gtccaggcag tggacgatga acggttgttg ctgttgcttg
      601 agctcagagc cgtatagatc gactaactct tcagcgaacg agagatcgcc agccccgatg
      661 tccaacacgg acgccacatc aatgtgacga aggaggtcaa aaggattctc cagatcgcgt
      721 acggcttcct cgacctcctc acttgataga taagtcacag gccacttcgc ggcggctgta
      781 ggaggcacca gctcaaaaaa gacggcaagc gcacgtaggg ctttggccgg aggaagaccc
      841 gtaacggact tgaggccttc tccgtcgaga tcctgaacgc gccgtgccgc atatcgttcg
      901 aagaggcgca acaaggtttc tttcactgaa gccggaagat ctttgctctg aacggcgtcg
      961 cacagtcgca cgatagtgga gggaaaggtg gtaccttcca gcaactttct tgatgcctcc
     1021 cattgtcggg gaaactgacg cgcacgaaaa cttacgacgc ggcggaattc agcgagtggg
     1081 tcggacctca tgaagcgtga tcggctgtgt agtgagaggg tatcaatcgg actctaaaga
     1141 ttcacgagca tagtaatctt caaacttcag cgtcaagtcc gcttgttcac gcttattc
//
LOCUS       DASTWE010001150         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_644823, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001150 DASTWE010000000
VERSION     DASTWE010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644823"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFT30_12005"
     CDS             <1..1154
                     /locus_tag="VFT30_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5093403.1"
                     /translation="IKQIPLDANSLSYELSRVTHELAEARVELQRFHQRLTVLHRLTD
                     ALVGSSDEEETIKVLMKGLPTLVDTVMIGIARSNRDRVWIWSDPQNREPEARVRRYLL
                     RRLRQSSSRLTGSHSSSRVVRSGHLYLVPPSASPQSMQEQDSTLGHEVPLALGPEEEG
                     LLLVQPKDQDDFTQSEREAVETVGAFLSLALRQAESHQRMQDLSLRDPLTEMLNTHAF
                     DGALARELSVGLRYGVHACLLLLDLDFFKTVNDRLGHTTGDHVIKTAAGLIRATVRKS
                     DIVGRYRGNIFAAVLPHTDMQQARALAERLRDCIERHPFAIEDGQVRTTASIGLAAIP
                     DTTVASVAEWAMIGDAALKDAKAHGRNRVVLHAPKPPGLACAMALSYAA"
     gene            1144..1779
                     /locus_tag="VFT30_12010"
     CDS             1144..1779
                     /locus_tag="VFT30_12010"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5093404.1"
                     /translation="MPHNVESGLTMTSLVTTPSAPSKNSADERREWIRIDDRVLMEYR
                     LLTETGSADPVEASSTTPEMISAAVAKPTADLLARTGESLIGSQVLPWILKVDYLLEV
                     ILNSLAVSQPSSVTMARPMDINLSGGGVGFVSPREFAAGDQLAIKLILPPFILIQALA
                     KVIRSVSQTDGQGFAIATEFVDLKAGDQEHLIRHILHAQAERLRARRNHAS"
     gene            1839..>2043
                     /locus_tag="VFT30_12015"
     CDS             1839..>2043
                     /locus_tag="VFT30_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5093405.1"
                     /translation="MAETQGILMNQLTIKEIIGNIEKRIDDHRLVNARITSEINILSL
                     NATIEAARAGDAGRGFAVVATEVK"
BASE COUNT          482 a          543 c          561 g          457 t
ORIGIN      
        1 tgatcaagca gatacccctc gacgcgaaca gcctatccta tgaattgtct cgggtcacgc
       61 atgagttggc ggaggctcga gttgaactgc aacgttttca ccaacggctt accgtgctgc
      121 atcgtctcac cgatgcactc gtaggctcgt ctgacgaaga agagaccatt aaggttttga
      181 tgaaaggatt accaactcta gtcgatacgg tcatgatcgg aatcgcaaga tcaaatcgcg
      241 atcgagtatg gatctggtcg gaccctcaga atcgcgaacc ggaggcgcgt gtgcgccggt
      301 atctgcttcg cagactccgc caatcctctt ctcgtcttac tggatcgcac tcttcttcgc
      361 gggtagtccg ttcagggcat ctttatctcg ttccaccttc ggcgtcacca cagtccatgc
      421 aagaacagga ttccaccctt ggacatgagg tgcctcttgc tctcggaccg gaagaggagg
      481 gtcttctcct tgttcagccg aaagatcaag atgactttac ccaaagcgaa cgagaagcag
      541 tcgagacggt cggtgccttt ctgtctctcg ccctgcgcca ggccgagtcg catcaacgaa
      601 tgcaggatct ttctcttcgt gacccactca cggagatgct caatacacat gcattcgatg
      661 gagcgttggc gcgtgaattg agcgtcggcc ttcgctacgg tgtgcatgcc tgtctgttgc
      721 tgctggactt ggattttttc aagaccgtaa atgaccgtct cggccacaca accggtgatc
      781 atgtaatcaa gacggcagcc ggtctgatac gggcaacggt gcgcaagagc gatatcgtcg
      841 gacgatacag aggcaatata ttcgcagccg tgctgcccca tacagatatg cagcaggctc
      901 gtgcccttgc ggagagattg cgagattgca ttgaacgaca cccttttgcg atcgaggacg
      961 ggcaagtccg taccactgca agtatcggct tggctgcgat tccagatacc actgtggctt
     1021 ccgttgcgga gtgggcgatg atcggcgatg ccgccctgaa agatgcgaag gcgcatggac
     1081 ggaaccgcgt ggtcctccat gccccgaaac cgcctggatt agcctgcgct atggcactga
     1141 gttatgccgc ataacgtcga aagtggcttg accatgacgt ctctagtcac aacaccttcc
     1201 gcaccatcca agaattccgc agatgaacgg cgtgagtgga tcagaattga tgaccgtgtt
     1261 ctgatggaat atcgtttgct gaccgagacg ggatccgctg atcctgtcga ggcgagcagt
     1321 acgactccgg agatgatttc cgcagcagtc gcgaaaccga cggcagatct gcttgcgcgc
     1381 acaggagagt cattgatcgg ctcacaggtg cttccatgga ttctgaaagt cgactatttg
     1441 cttgaagtca tcctcaattc tttggcagtc agccaaccgt cgagtgtcac catggcacga
     1501 cccatggata tcaatctgag cggtggagga gtggggttcg tctcgcctcg ggaattcgcc
     1561 gccggcgatc agctcgcaat caagttgatt cttccgccgt tcattctcat ccaggccttg
     1621 gcaaaggtga ttcgatccgt ctctcagacg gacggacaag ggtttgcgat tgccaccgaa
     1681 ttcgtagacc tcaaggcggg cgatcaggaa catctgatcc gccacattct tcacgcacag
     1741 gcagaacgtt tgcgtgcgcg ccgtaaccat gccagctaag ggaagactag ttcaataaca
     1801 tacggacatc actgaagaat tcatgtgatg aggtgaggat ggcggaaaca caagggatct
     1861 tgatgaatca gctcactatt aaagagatca tcggcaacat cgaaaagcgg atcgatgacc
     1921 atcggcttgt gaacgcaaga ataacgagcg agatcaatat tctttcgtta aacgcgacca
     1981 tagaagcggc acgagccggt gatgccggaa gaggattcgc cgtggtcgcg acagaggtga
     2041 aaa
//
LOCUS       DASTWE010001151         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1130011, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001151 DASTWE010000000
VERSION     DASTWE010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130011"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFT30_12020"
     CDS             complement(<1..220)
                     /locus_tag="VFT30_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5093406.1"
                     /translation="MAANPGFPLVLDVRSWPVLMIGGDEEAAEKSQRLLESGARVTVI
                     SPTLNEPLRLLAASGKIIHRGRHFRETDL"
     gene            complement(442..987)
                     /locus_tag="VFT30_12025"
     CDS             complement(442..987)
                     /locus_tag="VFT30_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5093407.1"
                     /translation="MEMTLLLNATYEPLRVVHWQKAIALLWQGKVEVLEVYDREIHGI
                     SLSIKLPAVMRLLKLVKLKDSHRAVKFSRINIFTRDGYCCQYCNHRFRTEELTFDHVV
                     PIAKGGKKTWENIVTACWRCNNRKSGRTPEEAGMKLKKRPTKPRWSPVITITIGIRNT
                     PESWRDYLYWNMELDADPADA"
     gene            complement(1050..>1267)
                     /locus_tag="VFT30_12030"
     CDS             complement(1050..>1267)
                     /locus_tag="VFT30_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093408.1"
                     /translation="KSALHVEDRNPNPIDFHGSSCAGWYLVREGYCDEIVHHGHLAIG
                     PPFAASAASDGHLLDFNATLWHRTISL"
BASE COUNT          290 a          347 c          331 g          299 t
ORIGIN      
        1 cgaggtccgt ttcgcgaaaa tgtcgtccac gatgaatgat cttcccagaa gccgccagca
       61 ggcgtagcgg ttcattcagt gtaggactga tgaccgtaac ccgcgcaccg gattcgagta
      121 ggcgttgtga tttttctgcc gcttcctcat cgccgccgat catcagaacc ggccagcttc
      181 tcacgtccaa aaccagggga aaaccaggat tcgcagccat gatggtactc ccgtttcgac
      241 caagtggttc agtatccgag aatggacctc tatcatgcaa gagggatttc aggaatccac
      301 actcatttcc cctttgatca gtccatcgct ataatgcagc gcgctggtaa gactggaatg
      361 acttcgtgcg aaacgttgga ggattagtcg agggatagag gttgcgaatg cgcagtcctc
      421 ataggtcttc aagatcgagc gttatgcatc ggccggatcc gcatccaact ccatattcca
      481 atagagataa tcacgccagc tttccggcgt gttgcgaatg ccgatggtaa tggtaatgac
      541 gggactccat cgaggttttg tgggccgctt ctttaacttc atgccggcct cttccggcgt
      601 acgcccactc ttccgattgt tgcaccgcca gcaggcggtc acgatattct cccatgtctt
      661 tttcccaccc ttggcgatag gcacgacatg atcgaacgtc agctcttccg tccgaaacct
      721 gtggttacag tattggcagc agtacccgtc tcgcgtaaaa atattgatcc gggaaaactt
      781 gacggcacgg tgactgtctt tcaacttcac cagcttcaag agccgcatca ctgccggaag
      841 tttgatcgac agggaaatcc catggatttc tctgtcgtag acctccaaga cttcgacttt
      901 cccttgccag agaagcgcga tcgctttttg ccaatgcacg acccgcaggg gttcataggt
      961 cgcattgagc aggagggtca tttccatgca ggaacctcat ccccaatcgg gcctccacag
     1021 cagccaggaa gcgtttccgc ggggctggac tacaggctga tagttctatg ccataaggtg
     1081 gcgttgaaat caagcaagtg cccatctgat gccgctgatg ccgcaaacgg cggaccgatg
     1141 gcgagatgac catgatgaac gatttcgtca cagtagcctt cacgcacgag ataccagccg
     1201 gcacaggacg aaccgtggaa gtcgatggga tttggattgc ggtcttcaac gtggagggca
     1261 cttttta
//
LOCUS       DASTWE010001152         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_644932, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001152 DASTWE010000000
VERSION     DASTWE010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644932"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFT30_12035"
     CDS             <1..561
                     /locus_tag="VFT30_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5093409.1"
                     /translation="RSFPADRARWLRVRDQIYEEIMQRGWNAKRGAFVQHYDSDSLDA
                     SNLIMPLVFFLSPNDPRMLDTLAAIARSPRSGGLVSDGLVYRYDSETGEDGLHGKEGT
                     FNMCTFWLVEALARAGRVDRAKLEEARFLFERMLSYANHVGLYSEETGTGGEALGNFP
                     QAFTHLALISAAFNLDRALGSRPKAG"
     gene            704..>1195
                     /locus_tag="VFT30_12040"
     CDS             704..>1195
                     /locus_tag="VFT30_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093410.1"
                     /translation="MNGSLRERQRSAIDSGRSVLLRNLILLGSIALSLLCVPYGFADE
                     SQSQGAATHMKVNGVVTKIQATHVDIKTHWGQMKIAAQVLPKGLEVGEEVEMWVNENN
                     AVIDVHRKGDPSHSHKFVTGNLVYTSMDKTEIKLHTPEGEKNFDVQTGKSKLSSIEEG
                     TPVT"
BASE COUNT          282 a          314 c          362 g          237 t
ORIGIN      
        1 cgctcctttc ccgccgatcg cgctcgttgg ctgcgggtgc gggatcagat ttacgaggag
       61 atcatgcagc ggggatggaa tgccaagcgc ggggcattcg ttcaacatta cgacagtgac
      121 tcgttggacg cctcgaatct catcatgccg ctcgtgtttt tcctttcacc gaacgacccg
      181 aggatgctcg ataccttggc ggcgattgcg cgctcgccga gatccggtgg actggtgtcc
      241 gatggcttag tgtatcggta cgacagcgaa acgggagaag acggcctgca tggaaaagag
      301 ggaaccttca atatgtgcac gttttggctg gtcgaagccc tggccagggc aggacgcgtc
      361 gatcgcgcca agcttgaaga ggcacgcttc ctgttcgaac ggatgttgag ttatgccaac
      421 catgtgggac tgtattccga agagactgga accggcgggg aagcactcgg taacttccct
      481 caagccttca cccacttggc gctgatcagc gccgccttca atcttgatcg tgcccttgga
      541 agcagaccga aggcgggatg atgctatgac gacgaacacc tctcgcaagt gtgagggccg
      601 gttgtagcag gaagctgatc tgtcatataa gtatgacgtt ccagtgggca cgtgtgaaac
      661 atgaaggaaa tagggtctgt ccattaacca agaggagggt atcttgaacg gttcattgag
      721 agagagacag agatcggcca tcgactcggg gagaagcgtc ctgctgcgca atctgatcct
      781 gctcggttcg atcgcattga gcctattgtg tgtgccctac gggttcgccg acgagagcca
      841 gagccaaggc gcggcgactc atatgaaagt caacggcgtc gtcactaaga tacaggcgac
      901 ccatgtcgac atcaagactc actggggcca gatgaagatc gccgcccagg tgctccccaa
      961 gggtctcgaa gtgggagagg aagtcgagat gtgggtgaac gaaaacaacg ccgtgatcga
     1021 cgtgcaccgc aagggcgatc cgagtcattc ccataagttc gtgacgggta atctggtcta
     1081 tacatccatg gacaagacgg aaatcaagct gcacaccccc gaaggcgaga agaacttcga
     1141 cgtgcagacc ggcaagtcga aactgtcctc catcgaggaa ggcacccccg tcacc
//
LOCUS       DASTWE010001153         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_95521, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001153 DASTWE010000000
VERSION     DASTWE010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95521"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFT30_12045"
     CDS             complement(<1..711)
                     /locus_tag="VFT30_12045"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU5093411.1"
                     /translation="MSAEPRSPRQRSVSPQLPLLGITMGDPAGIGPEVIAKALSGTHL
                     HNVCRSIVIGSFPVMERTIKALKLKLKVLRVHDHETTAPRRGTVAILDPLDTPLRTFK
                     LGIAATETGAASVAFIKKAVELAQIGCIQGMVTAPINKEAINMAGCQYPGHTELLADL
                     THANESGMMIVGGPLRIMFVTTHVAIKDLSLLLTQAKIEKAIRLAQLALTTLFGIKRP
                     RIGVAALNPHAGEHGLFGD"
     gene            complement(701..>1114)
                     /locus_tag="VFT30_12050"
     CDS             complement(701..>1114)
                     /locus_tag="VFT30_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093412.1"
                     /translation="LGLGGLIVGAHLFVMAAESMAGLFAMSPLILALLIAPLATELPE
                     MSNSFLWLYRKKDRLAVANVTGAMVFQGTFPVALGLIGTEWLIAPSALTTMILAVQAV
                     GLCLLQILIGGQWRPWLLAAGAIFYIGYTVHLYVS"
BASE COUNT          258 a          309 c          312 g          235 t
ORIGIN      
        1 atccccgaac agtccatgtt caccggcgtg gggattgagg gcagccactc ctatcctggg
       61 acgtttgatg ccgaacagcg tcgtcagcgc caattgagcc aagcggatcg ctttttcaat
      121 ctttgcttgg gtcagcagca acgagagatc tttgatcgcg acatgtgtcg tgacgaacat
      181 gatgcgtaac ggcccaccca cgatcatcat gcccgactcg tttgcgtggg tgagatcggc
      241 caacaattcg gtatggcccg ggtactggca accggccata ttgatggctt ctttgttgat
      301 gggagccgtc accattccct ggatacagcc gatctgagcc agttcgacag ctttcttgat
      361 aaaggcgact gaagccgcac cggtctccgt tgctgcaata ccaagcttga acgttcgcag
      421 cggtgtatcc agtggatcca gtatagccac tgttcctcta cgcggcgccg tcgtttcatg
      481 atcgtgaacg cggaggactt tcagtttgag cttcagtgcc ttgatcgttc gctccatgac
      541 aggaaacgat ccgatgacga tcgatcggca aacattgtgc aagtgagtcc cagatagagc
      601 cttggcgatc acttccgggc cgataccggc cgggtctccc atcgtgattc ccagcaaggg
      661 gagttgggga gatacggaac gttggcgggg tgatcgaggc tcagctgaca tagagatgca
      721 ccgtatagcc gatgtagaaa atggccccgg cagcgagtag ccacggtcgc cactgacctc
      781 cgatcagaat ctgcaagagg cagagtccca ccgcctgtac ggccaagatc atagtggtca
      841 atgccgatgg tgcaatcaac cattcggttc cgatcaaccc cagagcgacc gggaaggtgc
      901 cttgaaaaac catggctcct gtaacattgg ccacggcgag ccggtctttc ttgcgataga
      961 gccagagaaa gctgttggac atctccggca gttcggtggc gagcggtgcg atgagcaggg
     1021 ccaaaatcaa cggtgacatc gcgaacaagc ctgccattga ttctgcagcc atgacaaaca
     1081 aatgggcgcc cactatcaac ccacctaaac ccaa
//
LOCUS       DASTWE010001154         1370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1000937, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001154 DASTWE010000000
VERSION     DASTWE010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000937"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFT30_12055"
     CDS             <1..612
                     /locus_tag="VFT30_12055"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU5093413.1"
                     /translation="AAPFVRLIQSVGIVPQDRKQPFLKIMAPIEVAEGSKRVRIEPAS
                     TPRITYSIHYEHPLIKTQTYAHDCSVSAFENEISEARTFGFLYEVQALWARGLGKGGT
                     LDNTIVLSDQGVVNESGLRFDDEFVRHKILDLIGDFSLLGMPFIGHIVADRSGHSLHT
                     RLVQQILTQPDKWVLLNAEPSGEGKRSTAHALHLQPAVALQAS"
     gene            complement(842..>1370)
                     /locus_tag="VFT30_12060"
     CDS             complement(842..>1370)
                     /locus_tag="VFT30_12060"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5093414.1"
                     /translation="YEDFVEELTDILVGLEQRINAYKSRFQELEKKRRRLDDEIATIK
                     KYLELAETLYRVEADKAKLASLSNQIITPDDSKGIRSVPVMDVTDQSREILLGRSKYV
                     GKSVPQAAYEILRESNRAMHAKELVQRLIEGGLQIKGKTPLTSIATSLKRDKRFRKVG
                     PNTFEALDDMLIQAV"
BASE COUNT          271 a          373 c          363 g          363 t
ORIGIN      
        1 gccgcgccgt ttgtccgact gattcagtct gtcggcatcg ttccgcagga tcggaagcaa
       61 ccgtttctta aaattatggc cccgattgag gtggccgaag ggtcaaagcg tgttcggatc
      121 gaaccggcct ccaccccgag gattacttac tccattcact atgagcatcc tctgatcaag
      181 acgcagacct atgcgcatga ttgctccgtc agcgcgtttg agaacgagat ctccgaagct
      241 aggacgtttg ggtttctgta cgaagtccaa gcattgtggg ctcgtgggct aggcaagggc
      301 gggaccctcg acaacaccat tgtgctgtct gaccagggag tcgtcaatga atccggcctc
      361 cgattcgacg atgagtttgt ccgccacaag attctcgatc tgatcggtga cttttctctt
      421 ctggggatgc ccttcattgg ccacattgtc gctgaccggt cggggcattc acttcacact
      481 cggttggtcc agcagatcct gacgcagcca gataagtggg tgctcctgaa tgcggagccc
      541 tcaggagaag ggaagcgatc gaccgcgcac gcgcttcacc ttcagccggc cgttgcactt
      601 caggcttctt agtcaataga gttcggggga gtttctgggc atagtgctgg ttgcggttct
      661 tccgggcggg aggcgcttct cattccagaa catcgcagtg tggtacgccg ggggcaagtg
      721 gccttgccct cggcgtatca atcatggcgg ggattacttc tttttcttct ttgctgcctt
      781 cttcgttgcc aagagtctca ccccccttcg cactgtcaag ttagattcgt tatctgatcg
      841 gttacacagc ttgaatcagc atatcgtcca aggcttcgaa tgtattggga ccgacttttc
      901 taaagcgttt gtcacgcttg agcgacgtcg caatcgacgt gagcggcgtt ttccctttaa
      961 tctgcaaccc gccttcgatt aagcgctgca ccagttcttt cgcgtgcatc gcgcgattcg
     1021 attcacggag gatttcgtag gctgcttgag ggacactttt ccccacgtac ttacttcgtc
     1081 cgagaagaat ttcccgcgat tgatcggtaa cgtccatgac cggcacagac cgaattccct
     1141 tggaatcatc cggtgtaatg atctggttag agagactggc gagcttggcc ttgtcggcct
     1201 cgacacgata gagggtttca gcaagctcaa ggtatttctt aatggtggcg atttcgtcat
     1261 caagtcggcg acgcttcttc tccagttcct gaaaccgact cttgtacgcg ttgatccgtt
     1321 gctccagacc aacgagaatg tcagttaatt cctccacgaa atcctcatat
//
LOCUS       DASTWE010001155         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_548420, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001155 DASTWE010000000
VERSION     DASTWE010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548420"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFT30_12065"
     CDS             <1..223
                     /locus_tag="VFT30_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093415.1"
                     /translation="RPSTGHCCTITIQQSLYRQYDEREVVLMKNKRRQPALKTLSLAY
                     RTIGSESKWIQAGSKGIHPLWSSGFQREG"
     gene            complement(227..970)
                     /locus_tag="VFT30_12070"
     CDS             complement(227..970)
                     /locus_tag="VFT30_12070"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HEU5093416.1"
                     /translation="MRYALVLGLGLLLTASGVYILDPAEKAIAAKADQQDDSQSLIYT
                     PPKKFTPRARVGGTLRGTEGTEPELVALVPDHVGLTIKQTPTLNWYLSKPTTYSLRFT
                     LNDIQKVVPVYEGSIPAPTEAGVQSIDLKSLGLTLEPNVQYRWFVSASPNPQSPSRDI
                     VAGGMIERCELSECLTITSVNLTCDRESVRTNALIGFWYDAMGCICSLIERDPKDPSL
                     RRLRAALLRQVGLNGVADWDLRSIQSSGK"
BASE COUNT          257 a          282 c          291 g          243 t
ORIGIN      
        1 ccgaccaagc acaggtcact gctgtacaat tacgatacag caatctctat accgtcaata
       61 tgatgagcgt gaagtcgtac ttatgaaaaa taaacgccga caaccggcac ttaagaccct
      121 cagccttgct tatagaacta tcggcagtga atcaaaatgg attcaggctg gatcaaaagg
      181 gattcatcca ctatggtcat caggattcca gcgagaaggt tagcaactac ttcccgctgc
      241 tctgaatgga gcgaagatcc cagtccgcta ccccgttgag tccgacctgc ctgaggaggg
      301 ccgcgcgcag ccgccgaagc gacggatcct ttggatctct ctcaatcaac gagcagatac
      361 agcccatcgc atcgtaccag aatccgatga gggcattggt gcgcacgctt tcacggtcac
      421 aggtcaggtt taccgaggtg atcgtcagac attcactgag ctcacatcgc tcgatcattc
      481 cgccggcgac gatatccctg gagggggact gtggattggg actggccgac acgaaccagc
      541 ggtattggac atttggttca agtgtgagcc ccaggctttt gagatcgatg gattgaaccc
      601 cagcctcggt tggagcggga atggaacctt cgtaaaccgg aacgactttt tggatgtcgt
      661 tgagtgtgaa ccgcaaggaa taggtggtcg gtttcgagag gtaccaattc aacgtcggcg
      721 tttgcttaat ggtcaggccc acatggtctg gaaccaaggc gacgagttcc ggttccgtgc
      781 cctccgttcc ccgtaacgtg cctccgactc gcgcacgggg ggtgaatttc ttcggcggtg
      841 tgtagatgag agactgtgag tcatcctgtt gatcggcctt tgccgctatg gccttttctg
      901 ccgggtcaag gatatagact cccgaggcgg ttaggagaag acccaaccct aggaccaatg
      961 catatctcat ggtgattctc cttatcacag ccaattattg agcaacagaa aaggcgacca
     1021 gtaggccggg tgttcataaa tcctgtcgct caacagctta aactgcgcgc gct
//
LOCUS       DASTWE010001156         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_968351, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001156 DASTWE010000000
VERSION     DASTWE010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968351"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..565
                     /gene="rsfS"
                     /locus_tag="VFT30_12075"
     CDS             146..565
                     /gene="rsfS"
                     /locus_tag="VFT30_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5093417.1"
                     /translation="MARASKATALAVARAMLDKKAVDVQVLHVAPLTSIADYLVIGSA
                     ESDRQTRAIADSVADVLSRVGQRPLSLEGTTSGQWVLIDFGDVVAHVFRHDTRSHYAL
                     ERLWSDARSIPIPDNASTSTATPKRHVILKATSRKMV"
     gene            569..1969
                     /locus_tag="VFT30_12080"
     CDS             569..1969
                     /locus_tag="VFT30_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5093418.1"
                     /translation="MFKLLITIFLISAGVFIYSYFRELNPGTVVVHTAPGAEFELSPV
                     TLVLISVAFGAVLATLVVGLQQTAHLILNWRSNRLVRRKEKVDTLHRDGTHAFMSKRT
                     IEAITLLEKALAIDPNRVDSLLWLGNIYRSEQNYPEAIRLHQHAQRVDDRNIEVLLEL
                     GKDLEEAKRYEEALQTLQKILKIEPDNLTALIRKRNLNIRMERWSEALEIQHRLLKAN
                     LPTPEQQAEAALLVGCMYEVGRQLLERGHPDKARRYFRGAIKKDRSFLPAYIGMGEIL
                     LHEGKTKDAVEILKKVYSRTRSVIILHRLEELFLDQGEPSEIIRVYQEALQQDPHNPV
                     LQFYLGKLYYRLEMVDEAFDLLSTIEGPQDHLLDYHKIMANLYLRKQHFEEAIVELKK
                     ALSFKKRVVVPYICTQCQQESVEWSGRCRRCAKWNTLTALPWLEAGHTAASSAGEPSS
                     VRAVPYQGIASPFETV"
BASE COUNT          475 a          578 c          515 g          442 t
ORIGIN      
        1 atcacctgcc tggccattcc accctgtcca acctcggcct cagaaattag acagagagtt
       61 cggaatggac taccgctggc aaatatgttg ccccccccgg tagaatccta tatacttcgt
      121 catagccttt atcaggagga caacgatcgc acgcgcatct aaggccaccg ccctagccgt
      181 cgccagagcg atgctcgaca agaaggccgt tgatgtccaa gtcctccatg tcgccccgct
      241 tacttcaatc gctgattact tggtcattgg gtcggctgaa tccgaccgac aaacacgcgc
      301 gatcgctgat tccgtcgccg atgtactctc gcgcgtgggc caacggccac tgagtctcga
      361 aggcacgaca tcgggccaat gggtcctgat cgattttggc gatgttgtcg cccatgtatt
      421 cagacatgac acgcgttcgc attatgctct tgagcgtctg tggagcgacg cccggtctat
      481 cccgattccg gataatgcgt cgacttcgac tgccacaccg aagagacatg tgatcctgaa
      541 ggcgacttca cggaagatgg tctagaccat gttcaaactg ctgatcacca tttttcttat
      601 cagtgccggc gtgttcattt acagttattt ccgcgagtta aaccccggaa cggtcgtcgt
      661 ccataccgcg ccaggcgccg agtttgagct cagtcctgtc acacttgtgt tgatttccgt
      721 ggcatttgga gcggtactcg cgactcttgt cgtgggattg cagcaaacgg cacacctgat
      781 cttaaactgg cgcagtaacc gccttgtccg ccgcaaagag aaggtcgata cactccaccg
      841 agacggaacc catgcgttta tgtcgaaacg caccatcgag gcgatcaccc tcttggagaa
      901 ggccctggcc atcgatccta atcgagtcga ttcgctcctg tggctgggaa atatctaccg
      961 atcagagcag aattatcctg aggcaattcg gctccatcag catgcgcaac gggtggacga
     1021 ccggaacatc gaagtcttgt tggagttagg caaggacctg gaagaagcca aacgttacga
     1081 ggaagcgctt cagacgctcc agaaaattct caaaattgaa ccagataacc taacggcgct
     1141 cattcggaaa cggaatctca atatccgtat ggagcggtgg agtgaagcac ttgagattca
     1201 acaccgcttg ctcaaagcca atcttcccac ccctgagcaa caagcagaag cggcattgct
     1261 ggtcggatgc atgtatgaag tcgggcgtca actactcgaa cgtggacatc ccgacaaagc
     1321 ccgtcgatac ttccgaggcg ccatcaagaa ggaccgtagt ttccttcccg cttatatcgg
     1381 gatgggcgag attctgctcc acgaaggcaa aaccaaagat gctgtcgaaa tcctcaagaa
     1441 ggtctactca cggacgcgaa gcgtgatcat tctgcatcgg ctggaagaac tcttcttgga
     1501 tcaaggtgag cccagcgaaa tcattcgagt ctatcaggaa gccttgcagc aagacccgca
     1561 caatcccgtc ctccagttct atctaggcaa gctctactat cggctggaaa tggtggatga
     1621 agcgttcgac ctgctgtcga cgatcgaagg cccgcaggac catctgttgg actaccacaa
     1681 gatcatggcc aacctctatt tgcggaaaca gcatttcgaa gaggctatcg ttgaattaaa
     1741 gaaggccctc agcttcaaga agcgcgtggt cgtcccttat atctgcacac agtgtcagca
     1801 ggaatccgtc gagtggtcgg gccgctgccg ccgctgcgcc aagtggaaca cgctcaccgc
     1861 ccttccctgg ctggaagccg gtcacaccgc agccagctca gctggagagc ccagctccgt
     1921 ccgtgctgtt ccctatcaag gcattgcttc tccgtttgaa accgtgtagg tttcccaccg
     1981 gcgttcgcgg ataatgtgac ctgacaggtt
//
LOCUS       DASTWE010001157         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_871382, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001157 DASTWE010000000
VERSION     DASTWE010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871382"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1169
                     /locus_tag="VFT30_12085"
     CDS             <1..>1169
                     /locus_tag="VFT30_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093419.1"
                     /translation="PYWIGGAAGLGPSMGTGQTYAAIIIEATGYGHRNVFKHEWGHSI
                     LFYFDAMGLTPKPAVTNHAEVNQYVHWPTGDGYVWIDETDANPIPNSIYNNESGFTHD
                     YYSGTTATADQPERRLGITPEAWMLGGPVTKPGTSSVPPPVITCNADITVTSDPGTCQ
                     AIVKLIPPAVSDACDSSLTPVATRSDGADPWAVYPCGQTVVTWTVTNSANLTSTCEQV
                     ITVTDGKPPAFVHVPPPVTVTTGPDATSCGVVVDETRLGSTSVSNPTVPDPTGDVYNP
                     SSSLQNDIISTAATFDSDSITFTVQFADQIFPASSSNPRRLGGTIDIDTDQNSATGWH
                     PLVGPPALSLGIEFQINLWGERQHPGRVEVQTRFPDDDTFLGTVPIQFTGNSFSV"
BASE COUNT          260 a          354 c          313 g          242 t
ORIGIN      
        1 accctactgg atcggcgggg cagcgggatt agggccatca atgggtacgg gccagacgta
       61 cgcagccatc atcatcgagg ccactggata cggccaccgc aacgtcttca agcacgagtg
      121 gggacactcc atcctttttt actttgacgc aatggggttg acacccaaac cggcagtcac
      181 caaccacgcc gaggtcaacc agtacgtgca ttggcccacc ggcgatggct atgtgtggat
      241 tgatgagacg gacgcgaacc ctatcccgaa ttccatctac aacaacgagt cgggattcac
      301 acacgattat tattcgggga ctacggccac agccgaccag ccggagcgca ggctgggtat
      361 cacgcccgag gcatggatgc tgggtggtcc agtgacaaag ccgggcacgt cgtccgttcc
      421 gccgccggtg atcacgtgca atgcagacat cacggtcacg agtgatcccg gcacgtgtca
      481 ggctatagtg aaactcattc cgcccgccgt atctgacgcc tgtgacagta gtctcacgcc
      541 ggttgcaact cgcagcgacg gtgcggatcc gtgggccgtt tatccgtgcg gtcaaactgt
      601 cgtcacttgg accgtgacga atagcgcgaa tcttacatcc acctgcgagc aggtaataac
      661 agtgaccgat gggaagccgc cggccttcgt tcatgtcccg ccccctgtga cagtgactac
      721 tggaccggac gctacttcat gcggagtggt agtcgacgag actcgtctcg gaagcacttc
      781 cgtttcaaat ccgacggtgc ctgatccgac aggtgacgta tacaatccat catcttcact
      841 tcaaaacgat ataatttcga ccgcagctac attcgatagc gactcgatca ccttcacggt
      901 ccaattcgcc gatcaaatct ttcctgcttc ttccagcaac ccacgccgcc tcggaggcac
      961 gattgatatc gataccgacc agaattctgc aaccgggtgg catccgctcg tcggcccacc
     1021 ggcgttgagc ctcggcattg aatttcaaat caacctgtgg ggtgagcggc agcaccccgg
     1081 gcgtgtggaa gttcaaacca gatttccgga cgacgatacg tttctgggta cagtgccgat
     1141 ccagtttacc ggcaactctt tctcagtca
//
LOCUS       DASTWE010001158         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_936112, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001158 DASTWE010000000
VERSION     DASTWE010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936112"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /gene="zwf"
                     /locus_tag="VFT30_12090"
     CDS             <1..1080
                     /gene="zwf"
                     /locus_tag="VFT30_12090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5093420.1"
                     /translation="VPATLDLLDQTGLLKSQGWVRVVFEKPFGHDFHSARALNTLLHR
                     HLDESQIYRIDHYLGNETVQNLLAFRFANPIFESLWNRDTIERVEITVAEDLGVEHRG
                     AYYQEAGALRDMVQNHLTQLLTVVGMEVPTSFEASEIQAEKLKVLRSISPILPQDAIF
                     GQYTAWNVAGQRIDGYLEEPGVSPDSTTETYVALKLEIHNWRWRGVPFYLRTGKRLPR
                     KITQVAVTFRAAPIQVFRSLEPGSLNSNKLLITLQPSEGFSLCFSVKTPGRPFKFTDR
                     ALRFDYGQTFGELPEAYETLLRDVMIGDQTLFVTSDFTETAWRLYDPLLVGPRPVHVY
                     TAGSWGPKEADTLVERDGHSWQLGW"
     gene            1128..>2107
                     /locus_tag="VFT30_12095"
     CDS             1128..>2107
                     /locus_tag="VFT30_12095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5093421.1"
                     /translation="MPANPGTPVTTNIPQRMDRLPWARWHWLVTGALGITWLLDGLEV
                     SIVASLGPMLTHPDTLHLTQSEVGLTASAYLAGSVLGAFLFSYLTDRQGRKKWFMITL
                     ALYLTATVLTAFSWDLTSFLFFRFLTGAGIGGEYAAINSAIDELIPARSRGHTDLAIN
                     GTWWLGSAAGALMTLLLLNPAIFPESIGWRLCFVFGAVLGVAIIMVRRVIPESPRWLM
                     THGRVPDAEAIVSQIERQVAQDRGIVLADPQGTITVHTHPHATIATVARELFRSYPRR
                     TVLGIGLMITQSFMYNAVSFTYPLLLTKYYTVPSADIGLYILPFALGNFLG"
BASE COUNT          443 a          595 c          566 g          503 t
ORIGIN      
        1 gtgcccgcaa ccttggacct actcgatcaa accggcttgt tgaaaagcca gggatgggtg
       61 cgcgtagttt ttgaaaaacc ttttggtcat gattttcatt ctgcccgtgc actcaatacc
      121 cttctacacc gccatctaga cgaatcccag atttatcgca ttgatcacta tctcggaaat
      181 gagaccgtgc aaaatctatt ggcctttcgc ttcgcaaacc ccatttttga gtcgctgtgg
      241 aatcgcgata cgatagagcg cgtagaaatc accgtcgctg aagacctcgg tgtcgaacac
      301 cgcggagcct actatcaaga agccggtgcc ttgcgtgaca tggtgcagaa ccatttgacg
      361 caactactga ccgtcgttgg aatggaggtg ccgacttctt tcgaagcctc cgagattcaa
      421 gccgaaaaac tgaaggtgct tcgttctatt tcacccattc tcccccagga tgcgatcttc
      481 ggccaatata cggcgtggaa tgtcgccggc cagagaattg acggctacct ggaagaacca
      541 ggtgtatctc cggactcgac caccgaaacg tatgtggcgc tgaagttgga aattcacaat
      601 tggcgctgga ggggagtgcc gttttactta agaaccggga aacggcttcc gcgcaagatc
      661 acgcaagtgg ccgtcacctt ccgcgcggca cccattcaag tctttcgatc tttagagccc
      721 ggtagtctga actccaacaa actgctgatc accttgcagc caagtgaggg cttctcgctc
      781 tgcttctccg tgaaaactcc tgggcggccg ttcaagttta ccgatcgggc gctgagattt
      841 gactatggtc agacgttcgg cgagttgccg gaagcctatg agacgctgtt gcgcgatgtg
      901 atgatcggcg accagacgtt gtttgtcaca tcagacttta cggaaacggc ctggcgactc
      961 tacgacccgc ttttggtcgg tccccgaccg gtgcatgtct acacggccgg ctcgtggggg
     1021 ccaaaggaag cggacaccct cgtggaacgg gacggtcata gctggcaatt gggctggtag
     1081 tgattcacga tcaggtattc cgtccggttg tcatgctacg atggtgaatg cctgccaatc
     1141 ccggcacccc cgtcacgacc aatattccac aacgaatgga ccggttgcca tgggcacgct
     1201 ggcactggtt ggtcactgga gcattgggga tcacgtggtt gctcgacgga cttgaagtat
     1261 ccatcgtggc ctcacttggc ccgatgctga cccaccctga cacgttacat ctcacacaat
     1321 cagaagtagg actcacggcg tccgcctact tggcaggttc ggtcctcggt gcgttcctct
     1381 tttcttatct caccgatcgg caggggcgaa agaaatggtt catgatcacc ttggcgctgt
     1441 atctcacggc gacggtcctg acggcttttt cttgggatct cacgagcttc ctgttctttc
     1501 gatttctgac cggagccggc atcggaggtg agtacgcagc catcaattcc gcgattgatg
     1561 aattgattcc cgcccgaagt cgtggccaca ccgatctggc aatcaacgga acctggtggc
     1621 ttggatcggc ggcaggcgcc ttgatgacac tgctcttgtt gaatccggcg atcttccctg
     1681 aatccatcgg atggcggctc tgttttgttt tcggtgccgt gcttggagtc gccatcatca
     1741 tggttcggcg cgtcattccg gagagcccac gctggctcat gacccacggt cgggttcctg
     1801 atgcggaagc catcgtgtca cagattgaac ggcaagtggc gcaagatcga ggcatagtac
     1861 tggcggaccc ccaaggcacg atcacagtac atacccaccc ccacgcgacg atcgcaaccg
     1921 tggcgcggga actctttcgg tcttacccac ggcgaacagt tttaggcata ggattgatga
     1981 tcacacagtc gttcatgtac aacgccgtgt cgttcacgta tccgctgctg ctgacgaaat
     2041 actatactgt accaagcgcc gatatcggtc tctacatcct cccgttcgca cttggcaatt
     2101 ttctggg
//
LOCUS       DASTWE010001159         1533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1065545, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001159 DASTWE010000000
VERSION     DASTWE010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065545"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFT30_12100"
     CDS             complement(<1..234)
                     /locus_tag="VFT30_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093422.1"
                     /translation="MKKTPAKKVADAGKQLVRKGKALRGKAGATARGLKKTVADAVST
                     SSRRLKKGIKTAKSSPASRQARKVGEAVGTFLGQ"
     gene            689..>1533
                     /gene="ispH"
                     /locus_tag="VFT30_12105"
                     /gene_synonym="lytB"
     CDS             689..>1533
                     /gene="ispH"
                     /locus_tag="VFT30_12105"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5093423.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRQKGAVFVEELNEVPEGSVVIFSAHGVAKSVWEEANQRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYDLILIGHAGHPEVIGTLGQIPDKFHLVSSVKDVEELKVENTVNLSY
                     VTQTTLSVDECRDIVGALHQRFPNIKGPHQEDICYATQNRQNAVKELSRLVDVILVIG
                     SPNSSNSNRLRELGEQCGIPSYLIDSAADIDPAWLQGAKAVGIAAGASAPEILVTEVV
                     AFLRAS"
BASE COUNT          334 a          399 c          421 g          379 t
ORIGIN      
        1 ctggcccagg aaggtgccga cggcttcgcc gactttccgt gcctgacgac tggcgggaga
       61 ggacttcgcc gtctttatcc ctttcttgag ccgccggctc gatgttgata cagcatcagc
      121 aacggttttc ttaagtccac gagccgttgc gcccgccttg cctcgaagag cctttccctt
      181 tcgcacaagc tgctttccag catcagcaac ttttttagca ggtgtctttt tcataattga
      241 cctcctaggt ttgtgccgtc cgaccggtcc gcaaacataa caagcttgta tcagacgagc
      301 aaggactcct aggcacatct tctatttttc aggttcaaca gagcgcatac agatcagatt
      361 attttgaata ggtgacagtg tcgccgttgt gcggattcgc tgttggtccg tgccacgacc
      421 ctttcgtcgc atgatcaagg gagccaaagt gacggatcgg cacctgaatg gtcggttggt
      481 cttgtgttga actattaccc catcgcattc tgcacgacgg atggtggttg tgacgttggt
      541 taccggacct tttaacttcg aatcaatgat ggatgttgga cttctatgag ccaattctgt
      601 tgacggagcc tgctgatctg acgtatcgtg aatcttcacg ctaagcagat gaccctgtct
      661 agagttgagc taaggagcta cgacaaccat gaagatctat ctcgccaatc ctcgtggatt
      721 ttgtgccggt gtcgatcggg cgatcgatat cgtggatcta tctcttaaga aatacggtgc
      781 acctatttat gtccgccacg agatcgtcca tagccgtcat gtggtgaatt cgctccggca
      841 aaagggcgca gtcttcgtgg aagaattgaa cgaagtcccg gaagggtcgg ttgtgatctt
      901 cagcgcgcac ggcgtggcga agtcggtctg ggaggaggcg aatcagcgtc gtctccatgt
      961 gatcgacgcg acctgcccac tggtgatcaa agttcataac gaagtcaacc gcgattatac
     1021 ccagggatac gatctgatcc tcatcggtca cgccggccac ccggaagtga tcgggacgct
     1081 gggtcagatc cccgacaagt ttcacttggt gtcgtcggtc aaagatgtcg aagagctgaa
     1141 agtggagaac accgtcaacc tttcgtatgt cacacagacg acgctgagtg tggatgaatg
     1201 tcgggacatc gtcggcgcac tgcatcagcg gtttccgaat atcaagggtc cacatcagga
     1261 agatatctgc tatgcgacgc agaaccgaca gaatgccgtc aaagaattgt ctcgcttggt
     1321 ggatgtcatt ttggttatcg ggtcgccgaa cagctccaat tccaatcgtc tacgcgaact
     1381 gggagagcag tgcgggatcc cctcttatct catcgattca gccgccgaca tcgatcctgc
     1441 gtggctgcaa ggagccaagg cagtcgggat tgcggccggt gcgtcagccc cggaaatcct
     1501 ggtcacggaa gtggtcgcat tcctgagagc gtc
//
LOCUS       DASTWE010001160         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1485620, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001160 DASTWE010000000
VERSION     DASTWE010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485620"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="VFT30_12110"
     CDS             complement(<1..2007)
                     /locus_tag="VFT30_12110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEU5093424.1"
                     /translation="MEREVKTYVLKRPAEEAVARKLSINYAAALNPQQLAAVTAGEGP
                     SLVIAGAGSGKTRTLVYRVAYLIDSGVDPANILLLTFTRKSAQEMLERAGDLIGTSSH
                     RVCGGTFHSVANLLLRRYGRSIGIEPGFTILDRGDAEDLIALVRAQLGLNEKDKRFPR
                     KGTIMEMISKSENTLRSLDEIILEEFSHFADHSEDLGRLQNAYQTAKRQKQLLDYDDL
                     LVMLRQLLLLDEAARANISRQYRYILVDEYQDTNRLQAEVIRQLATTYQNVMVVGDDS
                     QSIYAFRGATFKNIMEFPLLFPGTTVYKLEENYRSTQPILNLANCIIEEATEKYTKRL
                     FTRKIDGPLPALVEAAGENAQSRFIAQKILELREEGVPLDEVAVLFRSSFHSFDLEIE
                     LSRKGLPFIKRGGVKFIETAHVKDLLAHVRVVSNPLDSVSWHRVLLLVDGVGPKKAQD
                     LLAAIVKSERPFDVLRGVSGRSGQGLKNLANTLESLAGSDDRQPAEQVNHIYEYYLPI
                     LKEQYDDYPKRTRDLDHLHTIAEGYEGIDEFLADLALEPPDGSAVDVEAFDRDDERLV
                     LSTIHSAKGLEWQCVFVIWVVDGRFPSAYSFLTDDELEEERRLFYVAVTRAKRHLFLT
                     YPINVYDKTSGMLLSKPSRFLDHVSSDLLDTLALVEEGGREDWGI"
     gene            complement(2085..>2393)
                     /locus_tag="VFT30_12115"
     CDS             complement(2085..>2393)
                     /locus_tag="VFT30_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEU5093425.1"
                     /translation="ILGLATRLQLPRIVLARMSLNLLINGTVGAVPIVGDLFSVWFRS
                     HTRNAALLREAAMRPDRETAVDWLYVSGIIGGTVATLLLAIAFVVWLVFKLWTMIAGG
                     "
BASE COUNT          566 a          719 c          589 g          519 t
ORIGIN      
        1 aatcccccaa tcctctcgtc caccttcctc taccaaagcc agtgtatcga gaaggtccga
       61 ggacacgtgg tccagaaacc gtgagggctt cgaaagcaac attccgctcg tcttgtcgta
      121 cacattaatg gggtatgtca aaaataaatg ccgtttggct cgcgtgacgg ccacatagaa
      181 gagtcgccgt tcttcttcca actcatcgtc ggtgagaaag gaataggctg atggaaacct
      241 gccgtcgacc acccagatga caaacacgca ttgccactcc aaccccttcg cggaatggat
      301 cgtcgaaagc acaagccgtt catcatcacg atcaaacgct tcaacatcca cggcactccc
      361 atctggcggc tccagcgcta aatcagcgag aaactcgtca ataccctcat agccctcggc
      421 aatcgtgtgg aggtgatcga gatctctcgt gcgcttcgga taatcatcgt actgttcctt
      481 gagaatcgga aggtagtact catagatatg attgacctgt tcggcgggct gccgatcatc
      541 cgacccggcc aggctctcca gcgtattggc caagttcttg agcccttgcc cggaacggcc
      601 gctcaccccg cgcagcacgt cgaatggccg ttcggacttc acgattgcgg cgagtaagtc
      661 ctgagccttc ttgggcccca ccccgtctac caacagcaac acgcgatgcc aactgaccga
      721 atcaagcgga ttggagacaa cccgtacatg tgccagcaga tccttgacat gggccgtctc
      781 gataaacttc acccccccgc gcttaataaa gggcaacccc ttgcgcgaga gttcgatttc
      841 caagtcaaat gaatggaaac ttgaacgaaa cagcacggcc acctcatcca gtggcacgcc
      901 ttcttcacga agctccagaa tcttctgtgc gatgaatcgc gattgtgcat tttctcccgc
      961 cgcttctact aatgcaggta gcgggccatc gatctttctc gtaaagaggc gcttcgtata
     1021 cttctcggtc gcttcctcga taatgcagtt ggcaagattc aaaatcggct gagtgctgcg
     1081 atagttttct tcgagcttat aaaccgtcgt gccggggaac aataggggga actccataat
     1141 gttcttaaag gtcgcacccc ggaacgcata gatagattga gaatcgtcgc cgactaccat
     1201 gacattctga taggtcgtgg ccaattggcg gatgacttcg gcctgcaagc gattcgtgtc
     1261 ttgatactca tcgacgagaa tatagcgata ctgacgggaa atgttcgcgc gggccgcttc
     1321 gtcaagcaac agcaactgcc gcagcatcac cagcagatca tcgtagtcca agagctgctt
     1381 ttgacgcttc gccgtttgat aggcgttttg gagccgtccc agatcctccg agtgatccgc
     1441 aaaatggctg aactcctcca ggatgatctc gtcgagactg cgtaacgtat tctcgctctt
     1501 gctgatcatt tccatgattg tccccttgcg ggggaaacgc ttgtcttttt cattcagacc
     1561 gagctgcgct ctcaccagag cgatcaaatc ctccgcatcg ccacgatcca gaatcgtgaa
     1621 tccaggctcg ataccaatcg atcggccata ccggcgcagt aacagattcg ccacggaatg
     1681 gaacgtccca ccacaaaccc gatgactgct ggtcccgatg agatcaccgg cacgctccaa
     1741 catttcctgc gcggacttgc gcgtgaacgt gagcaggaga atgttggcag gatccacgcc
     1801 ggagtcgatt agataggcca cgcgatagac cagcgtgcgc gtcttgccgc ttccggctcc
     1861 cgcgatgacc agcgaaggcc cctcgcccgc cgtcaccgct gccagttgct gggggttcaa
     1921 tgcagccgca tagttgatgg acaacttgcg cgcgactgcc tcttcagcag gccgcttcaa
     1981 cacataggtt ttaacttctc gttccatgga gatctgcgaa tgaggtgtcc gctaatttcg
     2041 ttactcagga aggttcgttg tatatcacac tcgacttccc tttcctatcc acccgcgatc
     2101 atcgtccata gcttgaagac caaccaaacg acgaaggcga tcgcgagaag cagggtcgcc
     2161 accgtgcctc cgataatgcc gcttacatag agccaatcaa cagcagtctc acgatccggc
     2221 ctcatagccg cctcgcgcag cagcgcggca tttcgcgtgt gactccgaaa ccagaccgag
     2281 aagagatcgc cgacaatcgg gactgcacct accgtcccat tgatcaatag attcagactc
     2341 atgcgagcca gcacaatccg gggaagctgg agacgcgtcg ccaacccaag aat
//
LOCUS       DASTWE010001161         1440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_354520, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001161 DASTWE010000000
VERSION     DASTWE010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354520"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..>1440)
                     /locus_tag="VFT30_12120"
     CDS             complement(86..>1440)
                     /locus_tag="VFT30_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5093426.1"
                     /translation="VLMIFSVILYAMTARNLRDAVDQSLEETATTAVRSLEERGFLPL
                     INEEELLSQFPELTRIDKFFQIFSPSGTISIRSPNIKQHEVPLSRTALDTAFAGQSIF
                     ESAKYPNEPPLRLISVPIMYRGNLLYIVQVGTSMESVGETLHRFLILLVVAIPIALTV
                     SLAGGWFLAGRALRPVDKITLAAQRIAAGDLSQRLSMPAAHDEIGRLAATFNNMIGRL
                     DVSFRQIRQFTSDASHELRTPLTVMKGETDLVLRRPRALEDYKSVLESNLEEIDRMTR
                     IVDELLFLSRADMGEVKMESLPVAMESLVEDIHRQATLLGQDRNIEVVLGTVMPVVVQ
                     GDDLRLRELLLNLVENAMKYSYPGGKVEISLLNDGREGRLSVTDHGIGIAPADHKRIF
                     HRFFRTDVARAHTKKGTGLGLAICAWIADLHKGRLEVKSDLGQGSTFTVVLPLAHPAV
                     "
BASE COUNT          336 a          406 c          399 g          299 t
ORIGIN      
        1 ggcacggcac gtctgtgaac gccaccgaga cggtgaggcg gcagtgtctc gcgagaacgc
       61 cgctggcgga cttttcaaca gcctgctaaa cagcgggatg agcgaggggc aacacaaccg
      121 tgaaggttga tccttggccg agatcgcttt tgacttctag ccggcccttg tgcaaatccg
      181 cgatccaggc acagatggca aggccgagac ccgtcccctt cttcgtgtgg gcgcgggcta
      241 catcggtacg gaaaaaccgg tgaaaaatcc gcttgtggtc tgccggagcg atgccaattc
      301 catggtccgt caccgagagc ctaccctctc gaccgtcatt caacagcgag atctcgacct
      361 ttcctccagg gtacgaatac ttcatcgcat tctcgacgag attgagcagt aactcgcgaa
      421 gccgcaagtc gtcgccttgc acaaccaccg gcataaccgt tcccagtaca acctcgatat
      481 ttcggtcctg ccctagcagc gtggcctgac gatgaatatc ttcgacgaga gactccatcg
      541 caaccggcaa cgactccatt ttgacttctc ccatgtcggc acgggagagg aacaacagtt
      601 cgtcgacgat tctggtcatt cgatcaatct cttccaaatt gctctcaagc accgacttat
      661 agtcttcgag tgcgcgaggt cgccgcaaaa ccaagtccgt ttcgcccttc atcaccgtga
      721 gaggggtccg caactcatgc gatgcatcac tggtaaattg acggatttgg cggaacgaga
      781 catccagtcg gccgatcatg ttgttgaagg tggcggccag gcgaccgatt tcgtcgtgag
      841 ccgccggcat gctgagacgc tggctgagat ctcccgcggc aatgcgctgc gcggcaaggg
      901 tgatcttatc gacaggacgt aacgctcgtc ctgccaagaa ccatcccccc gcgagagaaa
      961 cagtcaaggc gatcgggatt gccaccacaa gtagaatcaa gaatcgatgg agagtctctc
     1021 cgaccgattc catcgacgtg cccacttgta cgatatacaa gagattgccc cgatacatga
     1081 tcggcacgga gatcagccgc aatggaggct cattgggata cttggccgac tcaaaaatgc
     1141 tctgtccggc aaacgcggta tcgagggcgg tccggctgag aggaacttca tgctgtttga
     1201 tgttgggaga tcggatcgat atcgtgcccg atgggctgaa gatctggaag aacttatcga
     1261 tgcgcgtcag ttcgggaaat tgggagagca gttcctcttc attgatcaga gggagaaacc
     1321 cacgctcttc gagcgagcgc acagccgttg tcgccgtttc ttccagagat tggtcgacgg
     1381 cgtcgcgtaa attccgtgct gtcatggcat acagaatcac ggagaaaatc atcaagacca
//
LOCUS       DASTWE010001162         1395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_742131, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001162 DASTWE010000000
VERSION     DASTWE010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742131"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1395
                     /locus_tag="VFT30_12125"
     CDS             <1..>1395
                     /locus_tag="VFT30_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5093427.1"
                     /translation="VTIVLASLAAWSLLQFRGVMGILLASAVVVSYGALAIAMLSLTA
                     LVMPIANPLIASLFVFIGTAVWANLTASQRLILLERDMIRIQQESAAVREALVLHEDR
                     AEALQEDLETANAAIALSAGQQEELAKTTHLLRLQIAEAKSQEQDARRHLQELERQLG
                     DLRAASTVSSAIGDADLDRLQTESRQFGIITQDRSLLRLFHDLKKSAKSPLTVLLLGE
                     PGTGKELFARAIHRLSPRSEKTFIAVNMAAISPELFESELFGHVKGSFTGATAERRGY
                     FSLADHGTIFLDEIGDLRMDHQSKLLRVLQDKSFYRVGATTSTTVDVRIVAATNRDLR
                     KGVSEGWFREDLYFRLTGMVFHLPPLRERTGDIPLLADIFLNQFAQQIGKLAPRLTNE
                     ALRAMTEHGWKGNVRELRHCLEQAVTLNDETLLTGASLRLDEEHRQSRRRAEQTAPDL
                     ASDAAVLNCLRRHG"
BASE COUNT          313 a          392 c          383 g          307 t
ORIGIN      
        1 ccgtcaccat cgtgctggcc tcgctggctg cctggagcct cttacagttt cgtggggtga
       61 tgggcatttt gcttgccagt gccgtcgttg tttcgtatgg ggccttggcc atcgcaatgt
      121 tgagcctgac agctctcgtg atgccgatcg ccaatcctct cattgcctca ttgttcgtct
      181 tcatcggcac cgctgtttgg gcgaatctga cggcaagcca acgtctgatt ttgcttgaac
      241 gcgatatgat ccgcattcaa caagagtctg ctgccgtgag agaagcgctt gttctgcatg
      301 aggatcgagc ggaagctctt caagaagatt tggaaacagc caatgctgca atcgctttat
      361 cggccggcca acaagaagag ttggccaaaa cgacacactt actgcgactc caaatcgctg
      421 aagccaagag tcaggaacag gatgctcgtc gacaccttca ggaactggaa cgacagctgg
      481 gcgatcttcg ggcagccagc actgtatcaa gcgctatcgg cgatgcggac ctcgaccgcc
      541 ttcaaaccga atctcgacaa ttcggcatca tcacacagga tcgctccctg cttcggttgt
      601 ttcacgactt gaagaaaagc gcaaagtctc cgttgaccgt tctgcttctc ggagaaccag
      661 gcaccgggaa ggaactcttc gcccgtgcaa ttcaccgatt aagcccacgg tcggaaaaga
      721 ccttcatcgc cgtcaacatg gcagccattt ctccagagct gtttgaaagt gagcttttcg
      781 gccacgtgaa gggcagcttc accggagcga cggccgaacg tcgcggctat ttcagtcttg
      841 ccgaccacgg cacgatcttt ctcgacgaga tcggcgacct tcgaatggat catcagagca
      901 aactactgcg ggtgcttcag gacaaatcat tctatcgagt gggtgcgacc acctcaacga
      961 cggtagatgt tcgaatcgtg gctgcgacca atcgggacct gcggaaaggt gtgtcggaag
     1021 gttggtttcg cgaggacttg tacttccggc tgaccgggat ggtcttccat cttccgccgc
     1081 tgcgtgagcg caccggcgat atcccgcttc tcgccgacat ttttctgaac cagtttgctc
     1141 agcaaatagg gaagctggcc ccgaggctga cgaatgaagc gcttcgtgca atgaccgaac
     1201 atggatggaa aggaaatgtg cgggaactcc gccactgttt ggaacaggcc gtcacactga
     1261 acgatgaaac cctcttaacc ggagcctcac ttaggcttga cgaagagcat cggcaatcga
     1321 gaagacgtgc cgaacagacg gctcccgacc tcgccagcga tgcggcggtc ctgaactgtc
     1381 tgagacgaca tggct
//
LOCUS       DASTWE010001163         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1517916, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001163 DASTWE010000000
VERSION     DASTWE010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517916"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFT30_12130"
     CDS             <1..999
                     /locus_tag="VFT30_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU5093428.1"
                     /translation="MSELDIAERRVPQDGSFRMKLDRKTVDFRVSILPSVFGESVVIR
                     ILDRESIATGVSALKLERLGFNPEDLKRFRKAITRPYGMVLVTGPTGSGKTTTLYAAI
                     SEMNTLEDKLITIEDPVEYQLSGVVQIPVNEKKGVTFARGLRSILRHDPDKIMVGEIR
                     DAETAQIAIQSALTGHLLLTTVHANNVFDVIGRFASMGIDAYNFLAALNCVLAQRLIR
                     ILCPSCRTPVKVQQALIEESGLDYEHYKDTTFYEGEGCPQCHGTGYRGRKCITEFLDL
                     TDEIKEMILADRPLSEIRYRAVTDGMITLRQSALKKVLNGETSLREINRVTFSEEG"
     gene            1118..1999
                     /locus_tag="VFT30_12135"
     CDS             1118..1999
                     /locus_tag="VFT30_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093429.1"
                     /translation="MSPLSDGMIKPSPIEENVPDPSALADRIRTLTGSGSVHQAVGGA
                     ILSDLPRRIGVLLPDTAVRATVLHLEQLPVRREERDALIRWRLGQEQLFPLSGAKVLS
                     QVFHNRSGGKGPAHTVLTVAAQESVLKQYESLCESVGLIPQEVGITSLRIFDLWRRTS
                     NGPHWRSRECLWANVSDRALTTMIWQQGRLLFYRCKLLGAEAAEALTKTDMLNKILEE
                     CCASLEICQQQHPSAVVKEAVICADGDMSAFQELVEAELRLSVEQLSWESVESLGRMA
                     KGNHRGMASLAAMAGVL"
     gene            2001..2627
                     /locus_tag="VFT30_12140"
     CDS             2001..2627
                     /locus_tag="VFT30_12140"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU5093430.1"
                     /translation="MAIMSSTLARLFELKSSPLLVGANDQFQINLSRRYRPVVVPLRL
                     LLIGSCILLVIGICWNLGQAILTYQGSLTIQAELDRVRQQDLDLIAEARHEGIDLSEE
                     SLKRLPFEVELANQLLGKRTFSWTKFLTELEQAIPSHLALSSVRLDQAGTMVRLTGTA
                     TSFEDITAFTVGLQNHPTFKDPILVQHRVGPNGLVEFDVTLQYRHEGT"
BASE COUNT          651 a          670 c          779 g          634 t
ORIGIN      
        1 atgtcagagc ttgatattgc cgagcgccga gtacctcaag acggaagctt tcgcatgaag
       61 ctggatcgaa aaacggtgga ttttcgtgtc tccattctgc ccagcgtctt tggcgagtcg
      121 gtcgtgattc gcatattgga ccgggagtcg atagcgaccg gggtgtcggc tttgaagctt
      181 gaacggcttg ggttcaatcc cgaagacctg aagcgattcc ggaaagcgat cactcgcccc
      241 tacggcatgg tattggtgac agggccaaca ggaagcggaa agacaacgac gctgtacgcc
      301 gcgatctcgg agatgaacac gcttgaggac aagttgatta cgatcgaaga tcccgttgaa
      361 taccaactct cgggagtggt gcagattccc gtcaatgaaa agaaaggagt cacgtttgct
      421 cgcggccttc ggtccatact ccgtcatgat ccggacaaga ttatggtcgg tgaaattcga
      481 gatgccgaaa cggcgcagat cgccatccag tcggccctaa ccggccatct tctgctaacg
      541 acggtgcatg cgaacaatgt gttcgacgtc atcggccgtt tcgcgtcgat ggggatcgac
      601 gcctataatt ttctggccgc tctcaactgt gtgttagcgc agcggctcat tcgaatcttg
      661 tgtccgtcat gtcggacacc cgtcaaagta caacaggctc ttatcgaaga atcaggattg
      721 gactacgagc attacaaaga tacgacgttt tatgaaggag aagggtgtcc gcaatgccac
      781 ggaacagggt accgaggcag aaaatgtata acggaattcc tcgatttgac ggatgaaatc
      841 aaagagatga tccttgcgga taggccgctt tcagaaattc ggtatcgagc cgtgaccgat
      901 ggaatgatta cgctgcgaca atcagcgctg aaaaaagtat tgaatgggga aacgtcgctg
      961 cgagagatta atcgggtgac gttcagtgag gaaggttaac gcgatgtggg aatgggtcag
     1021 tagccgacca cagcgttgct tgaagttcgg agccgactcg atcgggtggg ccgaaactga
     1081 gcgaaattgg tgtgggcagc gtcgacataa gtgcgtcatg tccccgcttt ccgatggcat
     1141 gatcaaaccc tctccgattg aagagaatgt acccgacccc tcagcattgg cggatcgtat
     1201 tcgtaccctg actggttcgg gctcggttca ccaagctgtc gggggagcca tcctttccga
     1261 tcttcctcga cggattggag tgttgctgcc tgacacggcg gtcagagcga cagtcctgca
     1321 tctggagcag cttcctgtca ggcgcgaaga acgcgatgcg ctgattcgtt ggcgacttgg
     1381 gcaagagcaa ctgttcccgc tgagtggtgc gaaggtgttg tcccaggtat ttcacaatcg
     1441 gtcaggaggc aaaggtccag ctcatactgt gctgacggtg gcggcccagg aatcggtgct
     1501 gaagcaatat gagtctctgt gtgaatcagt ggggttgatt ccccaagaag tagggatcac
     1561 aagcctacgg atctttgatc tatggagaag gacttcgaat ggacctcatt ggcgaagccg
     1621 agaatgtctg tgggccaacg tatcggatcg agccctgacg accatgattt ggcagcaagg
     1681 gcgcttgctg ttctaccgat gcaagcttct cggggccgag gcggctgaag cgctcacgaa
     1741 gaccgatatg ctgaataaga ttcttgagga atgctgcgca tcgttggaga tctgtcaaca
     1801 acaacatcca tcagccgtcg ttaaggaagc cgtcatttgt gcagacggag acatgtcggc
     1861 atttcaagaa ttggttgagg cagagctccg actgtccgtc gaacaactaa gttgggaatc
     1921 ggtcgaatcc cttggtcgca tggccaaagg gaaccatcga gggatggcat cgttggctgc
     1981 gatggcaggg gtgctttagc atggcaatca tgagttccac cctcgctaga ctcttcgaac
     2041 tcaagtcgag tccattgctg gtcggagcca acgatcagtt ccagataaac ctgagccgtc
     2101 gttatcggcc ggtggtcgtt ccgctgcgat tgctcttgat cggcagttgc atcttgcttg
     2161 tgatagggat ttgctggaac ctcgggcagg cgatccttac gtatcaagga tctttaacca
     2221 tccaagcaga actcgatcga gtacggcaac aggacctaga cttgattgca gaggcccggc
     2281 atgaaggcat tgatttgtcc gaagagtcgt taaagcgact gcctttcgag gtcgagttgg
     2341 ccaatcagct gcttggaaaa agaacattct cgtggacaaa atttctgacc gaattagaac
     2401 aggcgatccc ttcacatcta gcactcagca gtgttcgcct tgatcaagcc ggcactatgg
     2461 tccgactcac aggaactgct acaagcttcg aagatatcac ggctttcacc gtcggactcc
     2521 agaatcaccc gacattcaag gatccgatct tggtacagca tcgtgttgga ccaaacgggt
     2581 tggtggaatt cgacgttaca ttgcaatacc gacacgaagg aacttgagcg tgaaagatcg
     2641 cctgctcttt ctctggcaac acccctttgc cccgttgctt ccatggacgg gagtcgctct
     2701 gggtcttatt ttcatgctgt tcctcatcca gaat
//
LOCUS       DASTWE010001164         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_742150, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001164 DASTWE010000000
VERSION     DASTWE010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742150"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFT30_12145"
     CDS             complement(<1..262)
                     /locus_tag="VFT30_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5093431.1"
                     /translation="MPRVARNTCDRYDLIVLPDDHVMGGGTASVTVITYCDFECLNCR
                     HAYILISGLRTRFADRVRFVYRHFPLVKKHPNAQLAAEASEAA"
     gene            complement(334..1140)
                     /locus_tag="VFT30_12150"
     CDS             complement(334..1140)
                     /locus_tag="VFT30_12150"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5093432.1"
                     /translation="MTANLSEKEQYRTNTQDLEWNEERAEFSHGENIGDVQRVLSAIA
                     GMIFVASGLSRLSRRNWAGASLATVGGSLLYRAISGYCPAFGALGIDMSGNKQAGGPS
                     DTSQLGRRTVHTGRATKIQRSIEINRPANELYRCWRSLDNLPRIMNYLDSVQVINDRL
                     SHWVVKTMPGAPMVEWDAEIINDVENRRIGWRSLKDADVDNTGSVEFKPTGENRTWLT
                     VTLQYEPPGGKLGTAVAKWFGEDPNTKIAEDLQRFKEQIETGVFSHADGR"
BASE COUNT          286 a          409 c          318 g          320 t
ORIGIN      
        1 cggcggcttc agaagcttca gcggcaagct gcgcattcgg gtgtttcttg actagaggaa
       61 agtgccggta gacgaatcgc acacggtcgg cgaatcgggt gcgcaatcca ctgatgagga
      121 tatacgcgtg cctgcagttg agacattcaa aatcgcagta ggtaataacg gtcaccgagg
      181 ccgttccgcc ccccatcaca tgatcgtccg gcaacacaat aaggtcatat cgatcacacg
      241 tgttgcgggc caccctgggc atagcgccta cacttcctcg tcaccgtcga tggaggtgat
      301 gctccaggaa cgtcatgtgt ggcatggcca ctcctatcga ccatcggcat gagaaaagac
      361 acccgtctct atctgttctt tgaatcgctg cagatcttcg gcaatcttgg tattcgggtc
      421 ttctccgaac catttggcta cagccgtgcc gagtttgccg cccggaggtt catactgcag
      481 cgtcacggtc agccacgttc tattttcacc cgtcggcttg aactccaccg aaccagtatt
      541 gtccacgtct gcatccttca gtgacctcca tccaatgcgc cgattctcca catcgttgat
      601 aatttcggca tcccactcca ccatgggggc accgggcatc gtcttcacca cccagtgaga
      661 cagccgatca ttgatgacct gcaccgaatc aagataattc atgatacggg gcagattatc
      721 caatgaccgc caacaccgat agagttcatt cgctggacga ttgatctcga tggatctttg
      781 aattttcgtt gcgcggcccg tatgcaccgt gcgccgacca agctgactcg tgtcgcttgg
      841 cccgcccgcc tgcttgttgc cgctcatatc gatccccaag gctccaaacg ccggacaata
      901 accacttatt gctcgataaa ggaggctccc tcccaccgta gccagcgacg cgcccgccca
      961 gttgcggcga gacaacctag acaacccgga ggctacgaag atcatccctg ctatggcaga
     1021 tagcacccgc tgcacgtcgc caatattctc tccatgactg aactccgctc gttcttcgtt
     1081 ccattctaaa tcctgcgtat ttgtacggta ctgctctttt tctgatagat tcgcagtcat
     1141 cggcttcctc cctcgttttc tccccatatc acgtttcttc ctcataattt ctgacgtgtc
     1201 tacctccctc gttgtgcaag aggcagaccg cctaccgcgt gagggcgaag acacaagggg
     1261 gtatcggccc catatttcct ggaaaagatt gcggccgtct ccataggggt aatcgcagat
     1321 tgaacttgag act
//
LOCUS       DASTWE010001165         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_257874, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001165 DASTWE010000000
VERSION     DASTWE010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257874"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /gene="pfp"
                     /locus_tag="VFT30_12155"
     CDS             <1..1167
                     /gene="pfp"
                     /locus_tag="VFT30_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEU5093433.1"
                     /translation="TIRSILGSLDVLGIIDGFKWLMEGSTGQVRPLSIEDVSRIHFQG
                     GSYLGTSRANPTRHAEALDNVLFSLSSLGVTRLMTIGGDDTAFSAMKLEERAGGSLQV
                     VHVPKTIDNDLDLPYGIPTFGFQTARHVGAEIVKNLMVDARTTTRWYLVVTMGRKAGH
                     LALGIGNAAGATLTIIPEEFRERPIKLQRVVDLLIGAMIKRLNQGRAYGVAVLSEGLI
                     EILDPRDLGGLEHVERDEYGHLRMNEVDVGDALRRELTKQLRQLGLSTGVVAKNVGYE
                     LRCADPIPFDIEYTRDLGYCAAQYLLDGGTSAMVSIQNGRFTPIPFKQMVDSSTGRTK
                     VRMVDINSQSYQIARQYMIRLMEADMNNRDMLSRYAALAGLSPETFRERFSTVL"
     gene            1205..2062
                     /locus_tag="VFT30_12160"
     CDS             1205..2062
                     /locus_tag="VFT30_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5093434.1"
                     /translation="MKILAATDGSKHGRWAIEWLAEMPFAVQPVVRVLHVIDVASVRA
                     PFMIQPMIVGTERYIQSEVKRMETAAKSTKKESEGLLSTLGLPGTVTIDQGGVAATIM
                     KHAQRGVGLLSIGSRGLDALDRFMLGSISNHALHHAPCSVLVVKEGSRPVRRVVLGID
                     GSAASDKAVKFLMRNVNPIPDGPDRKPIMVTVMHAVPYFKYPEVKEAGRTLVQHYGAK
                     LSKSGFQICEALRLGKPADEILTVAKQDKADLIVTGAKGLGAIRRILLGSVSTRVVQH
                     AHCGVLVVR"
BASE COUNT          458 a          542 c          652 g          453 t
ORIGIN      
        1 acgatccgca gtattcttgg gagcttggac gtcctaggga tcatcgatgg gttcaaatgg
       61 ctcatggaag ggagcacggg acaagtccgg ccgctctcga tcgaagatgt gagccggatc
      121 cattttcagg gaggatccta cctgggcacg tcacgtgcga atccaaccag acatgcggag
      181 gctctcgaca acgtattgtt ctcactatcg agcctcggcg tcacgcgctt gatgacgatc
      241 ggcggggacg atacggcgtt ttcagcgatg aagctggaag agcgagccgg cggcagtctt
      301 caagtcgtcc acgttcccaa gaccatcgat aacgatctcg accttcctta tggtattccg
      361 acgttcggat ttcaaacggc tcgtcacgtt ggggcggaga tcgtgaagaa tcttatggtg
      421 gacgcccgca ccaccacacg ctggtacttg gtcgtgacca tggggcgcaa agccggccat
      481 ctcgccttgg gtatcggaaa cgcagccggt gcaacgctga cgatcattcc ggaggagttt
      541 cgcgaacggc cgatcaaact ccaacgggtc gtcgatcttt taatcggggc catgattaag
      601 cggttgaatc agggtcgagc ctatggggtc gcggtgttgt ccgaaggact gatcgaaatt
      661 ctcgaccccc gtgatcttgg tgggttggag catgttgagc gagatgaata cggccatctg
      721 cgaatgaatg aggtggatgt cggcgatgcg ttgcggcgcg aactcacgaa gcagctgcgc
      781 cagttgggac tttccaccgg cgtggtggcg aagaacgtgg gctatgagct tcgctgcgcc
      841 gatccgatcc ccttcgatat cgaatacacg cgagaccttg gctattgcgc tgctcagtat
      901 ttactcgacg ggggaacctc ggccatggtc tcgattcaaa acggacggtt caccccgatt
      961 ccgttcaagc agatggtgga ttcctccacc ggacggacca aggtaaggat ggtagatatc
     1021 aattcccagt cctaccagat cgcccggcaa tacatgattc ggctcatgga ggccgatatg
     1081 aacaatcggg acatgctgag ccgctatgcc gccttggccg gcctgtctcc agagacgttt
     1141 cgagaacggt tcagcacagt tctctgaaca atggatgcat ttcgtcgaac aaaggatatc
     1201 cgatatgaaa attcttgcgg caactgatgg atcgaaacat ggcaggtggg cgatcgaatg
     1261 gttggcggag atgccgttcg cggtgcagcc ggtcgtccgt gtcctccatg tcatcgacgt
     1321 ggcgagtgtt cgtgctccct tcatgatcca accgatgatc gtcggtaccg aacggtacat
     1381 tcagtccgaa gtgaaacgga tggaaacggc ggcgaagtcc acaaaaaaag aatcggaggg
     1441 tttgttgtca acactcggct tgcccggaac cgtgacgatc gaccaaggcg gtgtggcggc
     1501 cacgatcatg aagcatgcgc agcgtggagt aggactcctg tcgattgggt cccgaggcct
     1561 ggatgccttg gatcgattca tgctgggcag catctcgaac catgcccttc accatgcgcc
     1621 ttgctccgta ctagtcgtga aagagggctc tcggcccgtc aggcgtgtgg tgttggggat
     1681 cgatgggtcg gcggcgtcgg ataaagcggt taagttcctg atgcgcaatg tcaatccgat
     1741 cccagacggt ccggatcgca agccaatcat ggtgacggtg atgcatgcgg tgccctattt
     1801 taagtatccg gaggtcaaag aggccgggag aacgctggta cagcattatg gtgctaagct
     1861 ctcgaaatca ggcttccaga tatgtgaggc cttgaggctg ggcaagccgg cggacgagat
     1921 tctgacggtc gccaaacagg ataaggcgga cctgatcgtg accggagcga aggggttagg
     1981 cgcgatccgc cgtatacttc tcggcagcgt ctccacccgt gtggtgcaac atgcccactg
     2041 tggagtgctc gtggtccgat gatcatctaa agtaggggat ggctcaaggg cggctaaaca
     2101 ggctt
//
LOCUS       DASTWE010001166         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1130426, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001166 DASTWE010000000
VERSION     DASTWE010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130426"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFT30_12165"
     CDS             complement(<1..391)
                     /locus_tag="VFT30_12165"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEU5093435.1"
                     /translation="MTWCCRLLIGGVLLASALGKSIDLKGFVDVLVTYRLFPSWSLWP
                     VALIIIGIEWILAAWILSGWRLRTGALFALMLNSFYALGLVVTLVRRLDLPNCGCYGV
                     FLPQPLRWYSPLEDLMLVGICYALHMGA"
     gene            complement(533..>1165)
                     /locus_tag="VFT30_12170"
     CDS             complement(533..>1165)
                     /locus_tag="VFT30_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEU5093436.1"
                     /translation="IVLQGMDTSGKDGTIKSVMSGVNPQGCKVVAIKVPSKDELDRDF
                     LWRVHREVPPKGYIGIFNRSHYEDVLITRVHGWVTDKAVKRRFNQIREFEELLIENGT
                     AVLKFFLHISKDEQKERLEARIADPEKRWKWNSGDLEERKMWSEYLKAFEDVILATST
                     EEAPWYVVPANRKWYRNLIVADRVVNALEDMKLKTPPDPEGVDFTKLRIE"
BASE COUNT          277 a          359 c          278 g          251 t
ORIGIN      
        1 gagcacccat gtgtaaggca tagcagatgc caactaacat aaggtcctcg agtggggaat
       61 accatcgcaa cggctgtggg aggaacacgc catagcaccc acagttcgga agatcgagtc
      121 tgcggacaag cgtgacgacg agccccaagg catagaagct attcagcatc agcgcgaaga
      181 gtgcaccagt ccgcaaccgc caaccggaaa ggatccacgc tgccagtatc cattcgatcc
      241 cgatgataat gagcgctacc ggccagagag accagctggg gaaaagccga taggtcacca
      301 acacgtccac aaatcccttg agatcgatcg atttccctaa cgcggaggcc aggagcactc
      361 ccccgatcag caggcgacag caccatgtca aaacggtcca cttcaacgaa gaccccagca
      421 taaataagaa gaggagagcg gaataaacgc cgcgctcctc cttctttcgc cttacctgcg
      481 actacgtgcg aatcgacggc accggccgac ccatcgtcgc ctcgacagcc cgctattcga
      541 tcctgagctt ggtgaaatcc acaccttccg gatccggggg tgtcttgagt ttcatgtcct
      601 ccaatgcatt cacgactcgg tccgctacga tgaggttgcg ataccacttt cgattcgccg
      661 gcacgacgta ccagggcgct tcctcggtgc tggtcgccaa gatgacatct tcgaaagcct
      721 tcagatactc actccacatc ttccgttcct ccaaatcgcc ggaattccat ttccagcgtt
      781 tttccggatc ggcgatgcgg gcttccagcc gctctttctg ttcgtccttt gagatatgga
      841 ggaagaactt gagcacggcc gtcccatttt cgatcaacag ttcttcaaat tctcttatct
      901 gattgaatcg acgcttgacg gccttgtcgg ttacccaccc atgtactcgc gtaatcaaca
      961 catcctcgta gtgcgatcgg ttgaagatcc cgatgtaccc tttgggaggc acctcgcgat
     1021 gtacacgcca cagaaagtca cggtccagtt catcctttga cggaacctta atggccacta
     1081 ccttgcaacc ttgcgggttg acgccggaca tgacgctctt gatggttcca tcttttccac
     1141 tcgtatccat cccctgcaac acgat
//
LOCUS       DASTWE010001167         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_871651, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001167 DASTWE010000000
VERSION     DASTWE010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871651"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFT30_12175"
     CDS             complement(<1..1326)
                     /locus_tag="VFT30_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5093437.1"
                     /translation="MADTATSTVRITIDGMTVNVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEIEKMPKLQTACSTPADEGMSIRTATTVVDDAHRSVLEFILANHP
                     LDCPVCDQGGKCDLQDFSHQYTATSRFVETKRIFQKDYFSPLIETQMNRCVQCLRCVR
                     YCDEVMDVKALAPVGRGTMTEIKHFGSHPLDCEFCGGCVQICPVGAITSRLSMYEYRP
                     WMLKRAETICGYCGDGCQITVQTKEQELIEVNSAHGAGRNNGDLCARGFFGFHAPSDP
                     HRLMHPLIRRDGVLGQATWEEALEYVAGRVGEIKAAHGGQSFGGLISGRCTNEELYLF
                     QKFLRVAVGTNSIDSSARYGHVNGLHAMQLVQGTHRWTVTFDDILDADVLILVGTNIT
                     ETNPITGLKVKEAVKKRRATLITIESLEPVVDTMSNIANLSHHHFRIPTD"
     gene            complement(1382..>1685)
                     /locus_tag="VFT30_12180"
     CDS             complement(1382..>1685)
                     /locus_tag="VFT30_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5093438.1"
                     /translation="RLMEFFYHESCGKCSPCREGSSWLVQTLRRILARRGRMEDLETL
                     LDLCKNIAGRTVCAFGDAAVAPIQSTLKHWRQEYVDLINEAEAANLIMPGQVGIGR"
BASE COUNT          344 a          476 c          463 g          402 t
ORIGIN      
        1 gtcggttgga atacggaaat gatggtgcga gagattggcg atattgctca tcgtgtcgac
       61 gaccggttcc agcgattcga tcgtaatcag cgtcgcccgg cgtttcttca ccgcctcttt
      121 gactttgagg cccgtgatgg gattcgtctc ggtaatgttc gtgccgacga ggatcaggac
      181 atccgcgtcc aggatatcgt cgaaggtgac ggtccaccga tgcgttccct gtacgagttg
      241 catggcatgc aggccgttga catggccata gcgcgcgctg ctgtcgatac tgttggtgcc
      301 gacggccaca cggagaaatt tctgaaaaag gtacaattcc tcgttggtgc aacggcccga
      361 gatcaatcct ccgaaactct gtcccccatg agcggcttta atctcgccga cgcggccggc
      421 gacgtattcc agcgcttctt cccaggtcgc ttgcccaagc accccatcgc gccgaatgag
      481 cggatgcatt agacgatgag gatcgctggg cgcatggaat ccaaaaaatc cccgagcgca
      541 taggtcgccg ttgttgcgtc ccgctccatg cgccgagttc acttcaatga gttcctgctc
      601 cttcgtctgc acggtgatct gacacccgtc tccgcagtaa ccgcagatgg tctcggctcg
      661 tttcagcatc cagggccggt actcatacat ggacagccgg ctggtaatcg ctccgacggg
      721 acagatctga acgcagcctc cgcagaactc acaatcgagc ggatgagagc cgaagtgctt
      781 gatttctgtc atggtgccac ggccgaccgg cgccaatgct ttgacgtcca tgacttcatc
      841 gcagtatcgg acgcagcgga ggcactgcac gcaacggttc atctgtgttt caatgagcgg
      901 actgaaatag tctttttgaa agatacgctt ggtttcgacg aaccggctgg tcgccgtgta
      961 ctggtgcgaa aaatcctgga ggtcgcactt ccccccttgg tcgcaaactg gacagtccag
     1021 gggatgattg gcaaggataa actcgagcac cgatctatgg gcgtcgtcga ccacggtggt
     1081 ggcggtgcgt atactcattc cttcgtctgc cggcgtgctg caagccgttt gaagcttggg
     1141 catcttttcg atttcgacca ggcacatgcg gcaattggcg tccggtttga gtttcgggtg
     1201 atagcagaaa tgcggaatca tgacgccgac gcgacgggcc gcttcgatca ccaatgtccc
     1261 tttcgggacg ttgaccgtca tcccgtcgat tgtgatgcgc acagttgatg ttgctgtgtc
     1321 tgccatgctc tccgttattc gttaaacgtt aaacatgatt cttgcgaata acgattcacg
     1381 attaacgtcc tattcccact tgccccggca tgatcagatt cgccgcttcc gcttcgttga
     1441 taaggtcgac gtactcttgc cgccaatgtt tcagcgtgct ttggatcggt gcgactgccg
     1501 catcgccgaa ggcacagacc gtgcggcctg cgatgttttt gcatagatcc aacaaggttt
     1561 caagatcctc catccggccg cgtctcgcca aaattctacg gagtgtctgg acgagccaag
     1621 aactaccttc tcggcaaggg ctgcatttcc cacaggactc atgatagaaa aattccatca
     1681 gccga
//
LOCUS       DASTWE010001168         1411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1421340, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001168 DASTWE010000000
VERSION     DASTWE010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421340"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..>1411
                     /locus_tag="VFT30_12185"
     CDS             34..>1411
                     /locus_tag="VFT30_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5093439.1"
                     /translation="MIGRSRRPEIDVGWIAREFGGGGHAVAAAATVKGQTLVEVKEKV
                     VRLLTTHYRPTLLAQDVMTRQVKAIEVDTSVTEAGQRMTAYGLNVFPILDEKDHYTGL
                     VSRESIQKALFHRLGKMAVRDIMQTDAYIAQPETSFHEIETAMIERNQRFVPIIKDKK
                     IVGVITRTDLLRTLHDDVLKAARMRTMRPSDAEAEIGKPRRNVKGLLRSRLPHRLVTV
                     LEDAGQLADRCQVPLFVVGGCVRDLLLGIENLDLDLVVEGDGIAFARKLGEVLHARVK
                     VHERFGTAILLSADGFRLDVATARTEYYEYPTALPTVEQSSIKKDLYRRDFTMNALAV
                     RLNGKGFGDVLDFYGGQRDLNDKVIRVLHGLSFVEDPTRVFRAIRFESRFGFRLGKDT
                     ATLIAGAVKMNLFHRLSGHRLLEELKLLLGEQKPKQAIKRLAELNLLKFIHPKLSWSD
                     RLDKLLAAI"
BASE COUNT          315 a          361 c          442 g          293 t
ORIGIN      
        1 ctcgtggcag tgatgatggc agatcgagtg gaggtgattg ggagaagccg caggcctgag
       61 atcgacgtcg gctggatcgc tcgggaattc ggaggaggag gccatgccgt ggcggcagcc
      121 gccacggtca aagggcagac acttgtggaa gtcaaagaga aggtcgttcg gctgctgacc
      181 acacactatc gtccgactct gctggcccag gatgtcatga cccgccaggt caaagcgatc
      241 gaggtcgata ccagcgtgac tgaagcagga cagcggatga cggcctatgg gctgaatgtt
      301 tttccgatct tggatgagaa agaccattac accgggctcg tcagtcggga gtcgattcag
      361 aaagccttgt ttcatcggct cggcaagatg gccgtgcgag acatcatgca gaccgacgcc
      421 tatatcgcgc agccggaaac ttcgtttcat gagatcgaaa cggccatgat cgaacgaaac
      481 caacggtttg tgccgatcat caaagacaaa aaaatcgtcg gcgtcattac gaggacggat
      541 ctgttgcgga ccctgcacga cgatgtgctg aaggccgctc gaatgcggac gatgcgcccg
      601 agcgacgccg aagctgagat cggaaagcca cgccgcaacg tgaagggcct gttgcggagc
      661 cgcttaccgc atcgcctcgt gacagtgctg gaagacgcgg gtcaattggc tgaccgctgc
      721 caggtcccgc tcttcgtcgt cggtggctgc gtgcgagacc tcttgctggg catcgagaac
      781 ctggatttgg atctcgtggt tgaaggcgac ggaatcgcct tcgcgcgaaa actcggtgaa
      841 gtgttgcacg cgcgggttaa ggtgcatgag cgattcggca cggctatcct gctgtcggcg
      901 gatggcttca ggctcgatgt ggccaccgca agaacggaat attacgaata tccgaccgct
      961 ctcccgacag tggagcaaag ttccatcaag aaagatctct atcgccggga ttttaccatg
     1021 aacgcgctcg ccgtccggtt gaacggaaag ggcttcgggg atgtgctcga tttctatggt
     1081 ggtcaacggg atctcaatga caaggtcatc cgagtcttgc acgggctgag ttttgtcgaa
     1141 gatcccaccc gcgttttccg cgcgatccga ttcgaatccc ggtttggatt tcgtttgggc
     1201 aaggacactg cgacgttgat tgcgggcgcc gtcaaaatga acctgtttca ccggttatcc
     1261 ggccaccgac tgctggagga actgaaactg ctgctgggcg agcagaagcc gaaacaggcg
     1321 attaaacgat tggcggagct caatctcttg aagttcattc atccgaagtt gagttggtca
     1381 gaccgattgg acaagttgtt ggctgcgata g
//
LOCUS       DASTWE010001169         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_451416, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001169 DASTWE010000000
VERSION     DASTWE010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451416"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1233
                     /locus_tag="VFT30_12190"
     CDS             <1..>1233
                     /locus_tag="VFT30_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093440.1"
                     /translation="ALIPGKALNRRGHAAGACIACALLFLSACVKHLEFPPLGDPLPA
                     SAKLEIPPSIKELTIRYSDSCGQLQEIPLGDRIQEALREGIHRTFKTTFDESTSETST
                     PDYVVQVDLVDSSFDINKEALYDRAPAVLQLNAIARIHDRKGTLLRQTDIKIARQERL
                     RLEQLSKNCDYMIEPFIRDAVIDFATRVSLDARLAVGGQDIASLEQTQMPVGNAGAPS
                     GSSVLRFKAMLLDENSNLIFEGGEHVRVRVDVVNAGTRSVENVSASLTGTQSIIEQFP
                     TATLQIQSLQAGQTKSLEFVATLPSTKQAQQAMIHVTVTESGGAVAAPQTLSLTIQPA
                     GTGTDDVNQIPSAAQGFHQPQTFLVSIGVGTYRDPKLVPRRYAATDAETVAAYFQSLG
                     GVPPSNIRLLQNQKALRA"
BASE COUNT          272 a          379 c          322 g          260 t
ORIGIN      
        1 tgcattgatc ccaggaaaag cgctcaatcg taggggacat gcggcagggg catgtatagc
       61 gtgtgcgctc ctctttcttt ccgcctgcgt caaacatctg gaatttcctc cgctggggga
      121 cccgttaccc gccagtgcca agctggaaat tcccccatca atcaaagaac tgacgattcg
      181 ctacagcgat tcctgtggac agcttcagga aattccgctg ggagatcgaa tccaagaggc
      241 cttacgggaa ggcatacatc gaacgttcaa gacgacgttt gacgaaagca ccagcgaaac
      301 ctccacgcct gattatgtcg tgcaggttga tcttgtcgat tcgtcgttcg acatcaataa
      361 ggaggccttg tatgaccggg cccccgccgt tttacaactc aacgccatcg cccgtatcca
      421 cgaccgaaag ggaacgttgc tccgtcagac ggacatcaag attgcccgtc aggaacggtt
      481 gcggctcgaa cagctctcga aaaactgcga ctacatgatc gaacccttta tccgcgacgc
      541 cgtcatcgat tttgcgacac gtgtctctct ggatgccaga ctcgctgtcg gtggacagga
      601 cattgcttcg ctggagcaga cccagatgcc ggtcggaaat gcgggagccc cttcaggatc
      661 gtcggttctt cgattcaaag ctatgctcct cgacgaaaac agtaatctga tttttgaagg
      721 cggcgagcat gtgcgggtcc gtgtggatgt ggtcaatgcc gggacgcgct ccgttgaaaa
      781 tgtgtcggct tctctcaccg gcactcagtc gatcatcgag caattcccca cagcgacgct
      841 gcagatccag tcactacagg cgggccagac gaaatccctc gagttcgtcg ccactctccc
      901 ctcgaccaaa caggcacaac aagccatgat ccatgtcaca gtcaccgaat caggaggagc
      961 cgtggcggcg ccccaaaccc tttcgttgac cattcagccg gccggtaccg gcacggacga
     1021 tgtgaaccaa attccctcgg cggcgcaggg ctttcaccag cctcagacct tccttgtctc
     1081 tatcggtgtc ggcacctatc gtgatcccaa gctcgtacct cgccgttacg ccgcgacgga
     1141 tgcggagacc gtcgccgcct attttcagtc gctcggcggt gtacccccat ccaacatccg
     1201 gttgttgcaa aaccagaagg cgctccgtgc cga
//
LOCUS       DASTWE010001170         1100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1582731, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001170 DASTWE010000000
VERSION     DASTWE010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582731"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..523
                     /locus_tag="VFT30_12195"
     CDS             53..523
                     /locus_tag="VFT30_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5093441.1"
                     /translation="MSVTQQHVLSRRGFCVCCAAAVGFAAKGGSFSPSQAYAQARTIV
                     DLIREDAAKAQIKIHKLRGNVSILEGSGGNVAVLTGADGKVFIDAGITATKPRILEAA
                     NSLSADPIKHLINTHWHFDHADGNEWLRGEGAAIIAHENTRKHLKISRAFGRLS"
     gene            complement(495..>1100)
                     /locus_tag="VFT30_12200"
     CDS             complement(495..>1100)
                     /locus_tag="VFT30_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093442.1"
                     /translation="PASPDHGIISPEQGEVARTSSLVTFRSLSYGFEQDMPYRLSVLF
                     HSMKVFSVDDEYGGEDLETKWTFLARVKGGNGIVKETKSTSWEQDPLEPRAEPYEIKR
                     KVGPLQLVSVDELVVMASGTDEDDPWNPDDDLAAITKSYRSNQSIWGSQTIILPPIDP
                     ANPSKTGIHFADAQNDEISYRLRYDINLEDSPSTTIFRTLC"
BASE COUNT          251 a          304 c          280 g          265 t
ORIGIN      
        1 atgcgctgct ttgggccctt ctttaaattg tatcgctaga aggaaactcg taatgtcggt
       61 cactcagcaa cacgtgctct cacgtcgagg tttttgcgtg tgttgcgcgg cggctgtcgg
      121 atttgccgcc aaaggcggtt cgtttagtcc ttcgcaggcc tacgcccagg cccgtaccat
      181 tgtcgacttg atccgcgagg acgccgcgaa ggcacaaatc aagatccaca agctgcgcgg
      241 caatgtcagc attctggaag gctcgggtgg caatgtcgcc gtgctgacgg gcgccgacgg
      301 aaaggtcttc atcgatgccg gcatcaccgc cacgaagccg cgcatcctgg aagcggccaa
      361 cagcctcagc gccgacccta tcaagcacct gatcaatacc cattggcact tcgatcacgc
      421 cgacggtaac gaatggctcc gcggcgaagg cgcggcaatc atcgcgcatg aaaacacgcg
      481 caagcacctg aagattagca gagcgttcgg aagattgtcg tagatggtga gtcctcgaga
      541 ttaatatcgt aacgcaaccg gtagcttatc tcgtcatttt gagcatcagc gaagtgtatt
      601 cccgtcttgc tcgggttagc tggatcaatg ggcggcaaaa tgatcgtttg cgagccccat
      661 attgactgat tgctcctata ggacttggta atggcggcaa gatcatcgtc tggattccaa
      721 ggatcatctt cgtctgtccc agaggccata acgacgagtt catcaacact caccagctgc
      781 agaggtccta ccttcctttt tatctcgtat ggctctgctc tgggctccaa ggggtcctgc
      841 tcccaactag tagattttgt ttccttaaca attccatttc cgcctttaac acgagccaga
      901 aaagtccatt ttgtctccaa atcttctcct ccgtactcgt cgtcgacaga aaatactttc
      961 attgaatgaa atagcaccga caacctgtat ggcatatcct gctcgaagcc ataggacagg
     1021 gagcgaaacg taaccagtga gctagttctt gctacttcac cttgttccgg cgagatgatt
     1081 ccgtgatcgg gtgacgcagg
//
LOCUS       DASTWE010001171         1434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_548804, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001171 DASTWE010000000
VERSION     DASTWE010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548804"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1434
                     /locus_tag="VFT30_12205"
     CDS             <1..>1434
                     /locus_tag="VFT30_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093443.1"
                     /translation="LSLTNDLAEARLRQQETTEMVSLLGGSDPMPVLVFPDIREQLIR
                     SRKGGTLEAGELRNCAVVLALMAEVERYAESHTVETQVLARVLAPLHDTRSLRGLLRA
                     IEDAIQSDGSMKDTASPELRRLTHQAQGLKQEMRQQLEQILHSKRYEDVLQESYFAQR
                     EGRYVVPVKTDMRGRIPGIVHDVSASGATVFLEPRELVELNNSIKVADLEVEREVQRI
                     LRKLTALVASNAARIEQGIEVLAECDVIRAKGEMSRRLKCNPVALNSDGRILLKQARH
                     PLLLIAKDQVVANDIHMDETVRVFVISGPNTGGKTVALKIVGLFALMVRVGLHLPCAP
                     ESEMALFIDLYADIGDAQDLSRDLSSFSAHMTYMIRLLSESISVSRSLVPSAPCALVL
                     LDEPVTSTDPQEGAALAEALLCRLAELNMKVVATTHYGALKELAQTTPGFVNASVEFD
                     VERLSPTYRLFLGIPGSSSALEIAGRLG"
BASE COUNT          302 a          369 c          445 g          318 t
ORIGIN      
        1 ctttccttga cgaacgacct cgcggaagct cgcctgcgtc aacaggagac gaccgagatg
       61 gtgagtttgc tgggggggag cgatccgatg ccggtgctcg tctttcccga tattcgagaa
      121 cagctgattc gatccagaaa ggggggaacg cttgaggccg gtgaattacg aaactgtgca
      181 gttgtgctgg cccttatggc ggaagtagag cgatatgccg agtctcatac agtcgagacc
      241 caggtcctgg ctcgggtctt ggctccgctt catgacacaa gaagtttgcg tggacttcta
      301 agggctatcg aggacgcgat ccaatcagat ggttccatga aggacacggc ttcgccggaa
      361 ctgcgacgcc ttactcacca ggctcagggg ttgaagcagg agatgcggca gcaactggaa
      421 cagatcctcc attccaagcg ctatgaggac gtacttcaag agtcgtattt tgcccagcga
      481 gagggacgtt atgtcgtgcc ggtgaagacg gacatgcggg ggagaatccc cgggatcgtc
      541 cacgatgtat ccgccagcgg ggcgaccgtc tttctggaac cgcgggagtt ggtcgaactg
      601 aacaattcca tcaaagtggc ggatctggag gttgagcgag aggtgcagcg catcttgcgt
      661 aagcttaccg cgctggtggc ttccaatgcg gcgaggattg agcaagggat cgaggtgctg
      721 gccgaatgtg acgtgatcag agcaaaaggc gagatgagcc gccggctgaa gtgcaaccct
      781 gtcgcattga atagcgatgg ccgtatcctg ctcaagcagg cgcggcatcc gttgctgctc
      841 atcgcgaaag atcaggttgt cgcgaacgac attcacatgg atgaaacggt ccgcgtcttt
      901 gtgatctcag ggccgaatac cggggggaag accgtcgcac tcaaaatcgt cgggttattc
      961 gcactcatgg tacgggtcgg actacatctc ccttgtgctc cggaatccga gatggcgcta
     1021 tttatcgatc tctatgcgga catcggggat gctcaggatt tgagccgcga tttgtcgagc
     1081 ttctccgctc acatgacgta tatgatccga ctcctttccg agagcatctc cgtttctagg
     1141 tcgctcgtac cctccgcacc gtgcgcactc gtactcctcg atgagcctgt gacttcaact
     1201 gatccgcaag aaggggcggc actggcagag gcacttcttt gccgtctagc tgaactgaac
     1261 atgaaggtgg tggcgaccac gcattacggt gcgctcaagg aattggcgca aacgacaccc
     1321 ggttttgtga acgccagcgt ggagttcgat gtggagaggt tgtcgcctac gtatcgattg
     1381 ttcttgggta ttcccggtag ttcatcggcc ttggagatcg ccggtcgcct gggt
//
LOCUS       DASTWE010001172         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_192886, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001172 DASTWE010000000
VERSION     DASTWE010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192886"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>1548
                     /locus_tag="VFT30_12210"
     CDS             38..>1548
                     /locus_tag="VFT30_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5093444.1"
                     /translation="MVHFRTRRGSDIRGLETFFSGGSFGYQKYGVAIGQETEKFDISM
                     FGSHVLEDGYIRHSNYNTQTLNFNVRYKIDDRQNFYFKAITNWLNAKVPSRLTQGQFF
                     ADERQAGGIRANCTPGTFRGGCADALLLNQSRVDRRTILGGIYERQIDTNTVLTVEAD
                     YDVKDIQQSFSQIGENTNPNYKSYADLRHDGRLGTMPLRSYIGFFVNQMEQKGNSFQN
                     LADGFGTKGTLLQNSRGSIFNIGGRIREELEFYSNWIFAVGLGFEQSRLSVHATNYDQ
                     TTGALANRASASRTFSNWAPEGALIWKPSVDRRHWIRASAGYGIPTFANLLRDPISGQ
                     PGTNFSLKPQKNLNFEIGTEMRLHPTLLVHVVGFYTFYKDEIITQVVNGNNTASVNAD
                     SSRYRGVEVFADWRPVTGLRVSGAYTHIDSEYINFSDRTAAGFIVRDGKQVPNVPTDI
                     LNGKVEYYHEPMGLGAWVEGNYSNSYFLNNNNTFGFPSYVIGNVNVYKNIELS"
BASE COUNT          388 a          389 c          411 g          360 t
ORIGIN      
        1 gtcctcttcc ctatacgaca actatgcatt gggtggaatg gttcacttca gaacgagacg
       61 aggaagcgac attcgtggac tggaaacgtt cttctcgggt ggatcgttcg gatatcagaa
      121 gtacggagtc gccatcggtc aggaaacgga gaagttcgac atttcgatgt tcggcagcca
      181 tgtgttggaa gacggctata tcaggcacag caattacaac acgcaaactc tcaattttaa
      241 cgttcgctac aagatcgatg accggcagaa tttttacttc aaggcgatta ctaactggct
      301 taatgccaaa gtgccgagca gacttacgca aggacagttc tttgcagatg aacgacaggc
      361 gggcggtata cgagcaaact gtacgcccgg caccttccgc ggcggctgtg ccgatgcact
      421 cctgctcaat cagagccgag tcgatcgccg tacgatcctc ggaggcatct atgagcggca
      481 gatcgatacg aacaccgtct taacggtgga agccgactac gacgtcaaag atatccaaca
      541 gtcattttcg cagattggcg agaacaccaa tccgaattac aagagctatg ccgatttgcg
      601 acatgacggc cggttgggaa cgatgccatt gcggagttat attgggtttt tcgtcaatca
      661 gatggaacag aagggcaatt cctttcaaaa tctggcggac ggcttcggaa cgaaaggcac
      721 attactccag aatagccgtg gatctatttt caacatcggc ggccgtatca gggaggagtt
      781 ggaattttac tcaaattgga tcttcgcggt cgggctcggc ttcgaacaat cccgtctcag
      841 cgtgcatgcc acgaactatg atcagaccac cggtgcgctt gccaatcggg ccagtgcaag
      901 ccgtaccttt tccaactggg cgccggaggg ggcgttgatt tggaaaccgt cggtagaccg
      961 tcgccactgg attcgagctt cggcggggta tggcatcccg acattcgcca atctcttgcg
     1021 agatcccatt tcgggtcaac cgggaaccaa cttcagtctg aaaccgcaga agaatctgaa
     1081 ttttgaaatc ggcacggaga tgaggctgca tccgaccttg cttgtccacg tggtcggttt
     1141 ctataccttc tacaaagacg aaatcattac tcaggttgtg aacgggaata acaccgcttc
     1201 agtcaatgcg gactcctcca ggtatcgcgg tgtcgaagta ttcgccgatt ggcgaccggt
     1261 gaccggccta cgtgtatccg gcgcctacac gcacatcgat tccgagtaca tcaactttag
     1321 cgatcgaact gctgccggtt tcattgtccg ggacggaaag caggtaccca atgtgccgac
     1381 cgacatcttg aacggcaagg tcgagtacta tcatgagccg atgggtttgg gcgcgtgggt
     1441 cgaaggcaat tactcgaata gctatttctt gaacaacaat aatacgtttg gctttccttc
     1501 ctacgttatc gggaacgtga atgtgtacaa gaatatcgaa ctctcgaa
//
LOCUS       DASTWE010001173         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_63748, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001173 DASTWE010000000
VERSION     DASTWE010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63748"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..340)
                     /locus_tag="VFT30_12215"
     CDS             complement(44..340)
                     /locus_tag="VFT30_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU5093445.1"
                     /translation="MTYIVAEPCVECKYTDCAAVCPVEAFHELPDKLLINPDTCIDCD
                     ACLPECPVEAIFSDMSIPEEYLSWLEINKEAENYPIISTKQPALYNAGCKGQPE"
     gene            490..>1009
                     /locus_tag="VFT30_12220"
     CDS             490..>1009
                     /locus_tag="VFT30_12220"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEU5093446.1"
                     /translation="MKAERELSLDFLRVCEAAAIESAKTMGQGDRKHSDHVAVEAMRE
                     VMDTVPMRGRIVIGEGERDEAPMLYIGEELGGKSYSEESRKDFPEVDIAVDPLEGTNL
                     CALGANNAIAVLAAAERGGLLNAPDIYMDKIVVGPSSRGAVDIDAPVEDNLKNIARRL
                     GRDVDDLTVICLD"
BASE COUNT          237 a          253 c          295 g          224 t
ORIGIN      
        1 cccagtcgga cagccctcta agacctccaa ctcaaatcta aagctattcc ggttgaccct
       61 tgcagccggc gttgtagagg gcgggctgct tggtgctgat tatggggtag ttctcggctt
      121 ctttattgat ctccagccaa gacaggtatt cctcgggtat agacatatcc gagaagatcg
      181 cctcgaccgg gcattcaggc aggcacgcgt cgcaatcgat gcaggtgtcg ggattgatca
      241 agagcttgtc cggcagttcg tgaaaggcct cgacggggca gactgcggca cagtcagtgt
      301 acttacattc gacacaaggt tcggcgacga tataggtcat ttttctactg agaactcctt
      361 ttcacaaggg tgaaaatcaa agtaaaaccc taatattcgg gggcatagct tgtcaaacgg
      421 gagcgtgtta gaatggctcg cataacttac gtttacaggt tcaataaggt acaggcataa
      481 caattatcta tgaaagcgga acgcgaatta tcgcttgatt tccttcgagt ttgtgaagcg
      541 gcggccatcg aatcggccaa aacgatgggc cagggtgacc gcaagcacag tgaccacgta
      601 gcggtcgaag cgatgcgtga ggtgatggat accgtcccga tgcgcggccg aatcgttatc
      661 ggcgagggcg agcgggatga ggctccgatg ctgtatatcg gcgaggaact tggcggcaaa
      721 tcgtattcgg aggaatcccg caaggacttt cctgaggtcg acatcgcggt tgacccgctc
      781 gaaggtacga atctgtgcgc ccttggtgcc aacaatgcca ttgccgtact tgcggctgcc
      841 gaacgcggcg gattgctcaa cgcaccggac atctacatgg acaagatcgt cgtcgggccg
      901 tcgtcgcgag gagccgtcga tatcgatgcg cccgtggaag acaatctcaa gaacatcgct
      961 cgccgtcttg gacgcgacgt cgatgatcta acggtgatct gtctggata
//
LOCUS       DASTWE010001174         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1033513, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001174 DASTWE010000000
VERSION     DASTWE010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033513"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..978)
                     /locus_tag="VFT30_12225"
     CDS             complement(400..978)
                     /locus_tag="VFT30_12225"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5093447.1"
                     /translation="MIATDRKIRCGWVGLKPHLIRYHDEEWGVPMHDDRKHFEMLLLE
                     GAQAGLTWETVLLRREGYRQAFAGFDPAVVARFTSRMKAALMKDVSIIRNRLKIEAAV
                     TNAQAFLAVQKEFGSFDRYVWRFVGGQPRINGWKTLSQVPAITTESEALSKDLKKRGF
                     RFVGSTIVYAYMQAVGLVNDHTSDCFLGGKVF"
     gene            complement(1070..>2144)
                     /locus_tag="VFT30_12230"
     CDS             complement(1070..>2144)
                     /locus_tag="VFT30_12230"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU5093448.1"
                     /translation="MFVSSIGALARAALEESAIGIGRFSLRLFTPVAPMLANSAEDVG
                     EALSRLEEAAWEYKLDGARIQVHKGGDEIKIFTRQLQDVTQRLPEIVEWAKHVPVREM
                     VLDGEALGLRSDGRPQPFQITMRRLGRQKDVATLQKEIPLSPYMFDALYLDGMPLLVQ
                     PYRDRIKVLDEVALSTRVPQIQTNDPDEARTFLQAALAAGHEGVMAKSLSAPYSAGRR
                     GIHWLKLKEAATLDLVILAAEWGNGRREGWLSNLHLGARDPESGQFVMLGKTFKGLTD
                     DMLRWQTEKLLSLETHRDVSTIFVRPELVVEIAFSDIQDSPRYPAGLALRFARVKRYR
                     TDKTAIQADTIQTVTELFKNQRA"
BASE COUNT          487 a          609 c          540 g          508 t
ORIGIN      
        1 aacgtagagt gccgacacag ttggagagaa caattcatca aagtgttcat cgaacgcagg
       61 gcaaagacca tcactagaaa ggagacaatc catgggattt agctttatca attggattgg
      121 attcgctatg ttggtgctgg cgttccttct caatgaggct ttccttggga taaacgcctg
      181 gtacgacctc aacataatgg cggtccttac gggtgtcgtg tggctggtcg ttgcggccgc
      241 attatcaata tggccaagcc acagggatca gagagcctga tcgattcttt tttcccgcct
      301 tgccaaaagg atcgagcgca ggggttaggt ctgtcctctg cgctttccga ccgtgatctc
      361 gctgaatttt tctaaaaaca ttgacgaagg gtggaatcgt tagaatacct tcccgccaag
      421 aaaacagtcg cttgtgtgat cgttcaccag cccgactgct tgcatatagg cgtagacgat
      481 tgtactgccc acaaatcgga agccgcgctt tttcaggtct tttgacaagg cctcgctttc
      541 agtcgttata gccggaacct ggctcaaggt tttccaacca ttgattcttg gctgcccacc
      601 gacaaatcgc cagacatacc ggtcaaatga tccaaactcc ttctgcacgg ccagaaatgc
      661 ctgtgcattc gttacggccg cttcaatctt caatcgattg cggataatac ttacatcctt
      721 catcagcgcg gccttcatcc gtgatgtaaa ccgggcaacg acagcagggt caaatccggc
      781 aaaggcctga cgatatcctt cgcgacgcag cagaaccgtc tcccatgtca ggcccgcttg
      841 agctccttcg agcaaaagca tctcgaagtg tttgcgatcg tcatgcatcg gcacacccca
      901 ttcctcatca tggtatcgaa tgagatgcgg tttcaggccg acccagccgc accgaatctt
      961 tctgtcagtt gcgatcatcg ccggtggttc cgcctctatc agatgcacag tagatacaca
     1021 gccgagattg taaaagcatc cggagaggag cacaacgagc ctgagtgtgt catgcccgct
     1081 ggttcttgaa caactccgtc acggtctgaa tcgtgtcggc ttgaatggcg gtcttgtcgg
     1141 tccggtaccg tttcaccctg gcaaagcgga gcgcgaggcc agctggataa cgaggcgaat
     1201 cttgaatatc actgaacgcg atctcgacga ccagctcagg tcgcacaaag atggtagaga
     1261 cgtcgcggtg agtctcaaga gaaagcaatt tttccgtctg ccagcgcagc atgtcatcgg
     1321 tcaatccctt gaatgtcttg ccgagcatca cgaattgacc gctctcggga tctcgcgccc
     1381 ctagatggag gttggatagc caaccttccc gtcggccatt gccccattct gcggcaagga
     1441 tcaccagatc aagcgtggcg gcttctttca gtttcaacca atgaattcca cgccgtcccg
     1501 cgctataggg agcactcaac gattttgcca tgactccttc atgaccggcc gccaatgctg
     1561 cttgaaggaa cgtacgtgct tcgtccggat cattggtctg tatctgtggg accctcgtgg
     1621 aaagagccac ttcgtccagg actttgatac gatcccgata cggttgaacg agcaatggca
     1681 taccgtcgag atacagtgca tcgaacatgt agggcgaaag agggatttcc ttctgaaggg
     1741 tcgccacgtc cttctgtcgg ccaaggcgcc gcatggtgat ctgaaacggc tgaggtcggc
     1801 catctgatcg caaacccaac gcttctccat ccagcaccat ctctcgaacc ggtacatgtt
     1861 tcgcccactc gacgatttcc ggcaatcgct gcgtcacgtc ttgcaactgt cgagtgaaga
     1921 ttttgatttc gtcaccgcct ttgtgcactt gaatgcgggc gccgtctagt ttgtattccc
     1981 aggccgcttc ctccaggcga ctcaacgcct ctcccacatc ttcagcgctg tttgccaaca
     2041 tcggtgcgac aggcgtaaac agcctcagcg agaatcggcc gatcccaatc gcgctctctt
     2101 ccaaggcagc gcgtgccagc gcaccgatgc tcgaaacgaa cata
//
LOCUS       DASTWE010001175         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_96153, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001175 DASTWE010000000
VERSION     DASTWE010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96153"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..715)
                     /gene="thiE"
                     /locus_tag="VFT30_12235"
     CDS             complement(41..715)
                     /gene="thiE"
                     /locus_tag="VFT30_12235"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5093449.1"
                     /translation="MAQVSGEKNPCASVLAGVYVILDPSICSDRPLVEILNVSATAGA
                     RIFQYRNKTASMKAAYAEALPLRKAAQELGVLFIVNDRCDLALAVDADGVHLGQGDLP
                     LDLARKVMGPDKLIGISTHSREQVVAATAGGPNYLGFGPIFMPGSKLDHDPVVGLQGL
                     RAIRPLTALPIFAIGGITADRTEEVIRAGADGVAVISAILKAPDISQAVTDFVLQIST
                     LTSPGS"
     gene            complement(721..1308)
                     /locus_tag="VFT30_12240"
     CDS             complement(721..1308)
                     /locus_tag="VFT30_12240"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5093450.1"
                     /translation="MRLSDNSLGVWLVLLLLIVVQVPLSIVALESWALAAADVDELEV
                     GAEPGGGVHATAHPLFPAKPEVIQALLSDYAHWPDLFEVRMKVVALNVHDGVATIDLR
                     IDHPVMPGERRLVTESRVLPNGGLVTDLKDGDFKRYHRVWKLQSAGDHTRADFELTVE
                     PDSLAPDWLVAMVVRQELAAHFRIVKQKALEHSKR"
     gene            complement(1292..>1641)
                     /locus_tag="VFT30_12245"
     CDS             complement(1292..>1641)
                     /locus_tag="VFT30_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093451.1"
                     /translation="FGMKADRCYALVRDYLKGVKDVMGEVWGQNDRYMFTRDVTLKAL
                     IRVLDDLIVDRKLINDWEEQRSHKPFAEIVKPWAPLTKEFRADGFYERFPAKGQLERV
                     RKIHQRLLDAIVG"
BASE COUNT          384 a          519 c          405 g          333 t
ORIGIN      
        1 ttccgccccg ccatcatcca tcttgctcga tctgctgtgc tcaagaacct ggcgaagtta
       61 gtgttgagat ctgcaaaaca aagtcggtga cggcctgcga aatatccggt gccttcagaa
      121 tagcggagat gaccgcgacg ccgtcggctc ccgctcgaat cacttcttca gtgcgatcgg
      181 ccgttatccc tccgatggcg aagatcggga gtgccgtcaa gggacggatt gctcgtaacc
      241 cctgcagacc aacgaccgga tcatggtcca actttgagcc gggcatgaaa atcggaccaa
      301 acccgagata gtttggtccg cccgctgtcg cggccactac ctgctctcgg ctgtgcgtcg
      361 aaataccgat cagtttgtca ggacccatga cctttcgtgc aagatcaagc ggtaagtctc
      421 cctgtccgag gtgcacaccg tccgcatcca cggccagcgc taaatcgcat cgatcattga
      481 cgatgaacag gacgccgagc tcctgtgccg ccttcctcaa cggcaatgcc tccgcatagg
      541 ccgccttcat cgaagccgtc ttgttccggt actgaaaaat tctggcgccg gctgtcgcgg
      601 aaacgttcag gatctctaca agcgggcgat cggaacaaat cgacggatcc agaatgacat
      661 agacgccagc taggaccgag gcgcaaggat tcttctctcc tgaaacttga gccatcacag
      721 ttaccgcttg gagtgctcca acgccttctg tttgacgata cgaaaatggg cggccaattc
      781 ttgccgcaca accatcgcca ccaaccaatc tggtgccagc gaatccggct caacagtcag
      841 ctcaaaatcc gcgcgtgtgt gatcccctgc cgactgaagc ttccagaccc tgtggtagcg
      901 cttgaagtca ccgtccttca gatcagtcac caggccaccg ttcggtaaga ctcgggattc
      961 cgtgaccaac cgacgttcac ccggcatcac gggatggtcg atacgaagat caatggtggc
     1021 caccccatca tgaacgttca aggcaaccac tttcatccga acctcaaaga gatccggcca
     1081 atgggcgtaa tccgacaaca acgcttgtat cacttccggc ttagcaggaa agagtggatg
     1141 tgccgttgca tgcacgccgc cgccaggttc cgcccccacc tccaactcat cgacatctgc
     1201 cgccgccaac gcccacgact ccaatgcgac aatcgacagc ggaacttgaa cgacgatcag
     1261 aagcaggaga accagccaaa cgccaagtga gttatccgac aatcgcatcc agcagccgtt
     1321 ggtgaatctt tcgtacccgt tcaagctgtc ctttggcggg aaaccgctca tagaagccgt
     1381 cggcccggaa ctccttcgtc aggggagccc agggtttgac gatctcagca aatggtttgt
     1441 gcgaacgttg ttcttcccaa tcattgatga gcttgcgatc cacgatcaga tcgtccagga
     1501 cgcgaatcaa cgccttgagc gtcacatcac gagtaaacat ataccggtca ttctgccccc
     1561 acacctcgcc catgacgtcc ttgaccccct tcaggtagtc gcgcaccagc gcatagcagc
     1621 gatctgcctt cattccaaaa t
//
LOCUS       DASTWE010001176         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_678116, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001176 DASTWE010000000
VERSION     DASTWE010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678116"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFT30_12250"
     CDS             <1..349
                     /locus_tag="VFT30_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin B12-binding/radical SAM family
                     protein"
                     /protein_id="HEU5093452.1"
                     /translation="AEFYAELVKTQQVLNKKHLGWAALKGTAKIAAGHLMRGQTNFIK
                     MLWKFNSVYNPELQMADHRRQVVYEMSPPPEKKDKLDAKQLYILPAKGRQGRAIDDST
                     ETFVDESRMGTAV"
     gene            complement(488..>2156)
                     /locus_tag="VFT30_12255"
     CDS             complement(488..>2156)
                     /locus_tag="VFT30_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093453.1"
                     /translation="KSSSPMDRFHQARVVLAHTTLTQPFLLAQTTIPDTSQDDYDVNN
                     PPMRRENAPPGWVGYKTTDTMTRIGKPPRTEGMYESYEMVIAPKAKQCPHLEGPDLKG
                     IADGTFEWSFLMYRKAMGQAVLYRRHVVATLKGEVDDEAKVKQVDFDVTVTLQHVGTE
                     LAPYSQSYGSRGHFTLDQRTSLPQEFRIITVSDFSEAGVQANDAHLIGTLTALVAYFS
                     GQEHYKAQQYWNHSNTCVDITFNPATKTQGFAPNKSYQVKTELRAKKEQTVVPAKFKE
                     AKERPREGNGTVSPREDKSQGDRPATFTYQAPGTRMRHSGFRVNAVSRAGVAEAKSGE
                     WELVESYVLEFKSHIVQEPLNIVNPQFGMQMSSNGFDAHVQATVPLQHTDDRGWVGEG
                     EMRYETRTMTQRAQCEIRIQGTGTTTFHVNGGSISSDPEPLAVNLVILPGRSGEVAET
                     HCTSANTPRKLRELLESQGAHVGDAHAVTEGGGWSSAFSFTRFRTFNWNKKGYEIGGW
                     TPVRDSDVIAKKTISVNCSTGMSSCREETTLILRLADEPGASASQPK"
BASE COUNT          431 a          627 c          565 g          533 t
ORIGIN      
        1 ggctgaattt tacgcggaac tggtcaagac tcaacaggtc ttgaacaaga aacatctcgg
       61 ctgggcggcg ctcaaaggta cggccaagat cgcggcaggg catttgatgc ggggacaaac
      121 caatttcatc aagatgctat ggaagttcaa cagcgtctac aatcccgagc tgcaaatggc
      181 tgatcaccgc cggcaagtgg tctacgaaat gtcgccaccg ccggagaaga aagacaagct
      241 cgacgcgaaa cagctgtaca ttttacccgc gaaaggccgc cagggccggg ctatcgacga
      301 ttccaccgag accttcgtcg acgaatcccg tatgggaaca gcggtctgat cgttgttgtc
      361 ggtgtcggtg ggatgtcagt cgccgtcatg gcttccctag gtcgaagaat gtactcgatc
      421 acagactcac cactgactcg accttcgcac cgacggccag aacccttacg cgaactcttg
      481 atgcttttta tttcggctgg gatgcgcttg ctcccggctc atccgccagt ctcaagatca
      541 gcgtcgtctc ttctctgcaa ctgctcattc ctgtgctgca gttaacagag attgtctttt
      601 ttgcgatcac gtccgagtcg cgtaccggtg tccagccacc gatctcgtag cccttcttat
      661 tccaattgaa tgtcctgaag cgagtgaagc tgaatgccga actccatccc ccaccttctg
      721 tcacggcatg cgcgtcaccc acatgggcgc cttgtgactc aagcaactcc cttaattttc
      781 tgggcgtatt ggcactcgta cagtgggtct ctgccacttc gcctgatcgt cctggaagga
      841 taaccaagtt cacggctaat ggttcaggat cactgctgat cgagccgccg ttcacatgaa
      901 aggtggtcgt gccggtgccc tgaatacgaa tttcgcactg agcccgctga gtcattgtgc
      961 gagtctcgta tcgcatctca ccttccccaa cccatccgcg atcgtccgtg tgctggaggg
     1021 ggactgtcgc ctggacatga gcatcaaacc cattcgaact catctgcatg ccgaattgcg
     1081 ggttcacaat attcaatggt tcctggacga tgtgcgactt gaactccagc acatacgact
     1141 ccaccaattc ccattcccca ctcttggctt ccgcgacgcc ggcgcgggag actgcgttga
     1201 cccggaatcc actatgccgc atccgtgtcc ccggtgcttg ataggtaaac gtggcgggcc
     1261 tgtcaccttg cgacttgtcc tctctcggtg agaccgtccc attgccctcc ctcggccgct
     1321 ctttcgcctc cttgaacttg gccggcacca ccgtctgttc cttcttagcg cgcagctctg
     1381 tcttcacctg gtacgatttg ttcggagcaa atccctgcgt tttggtggct ggattaaacg
     1441 taatgtcgac gcaggtattg gagtggttcc aatactgttg agccttgtaa tgctcttggc
     1501 ctgaaaaata ggccacaagc gccgtgaggg ttccaatgag atgcgcatcg tttgcctgaa
     1561 cccctgcttc agaaaagtcc gatactgtaa tgattctaaa ctcctgggga aggctcgttc
     1621 gctgatcgag cgtgaagtgg cccctgcttc cgtaggactg ggaatagggc gcaagctccg
     1681 tgccgacatg ttggagcgtg acggtgacgt caaaatccac ctgtttgact ttggcctcat
     1741 cgtcgacctc ccctttgagt gtcgccacga cgtgccgacg atagagtact gcctgtccca
     1801 ttgctttccg atacatcaga aatgaccatt caaaggtccc gtccgcgatt cctttcagat
     1861 ccggcccttc cagatgcggg cactgtttcg ctttcggggc aatgaccatt tcatagcttt
     1921 cgtacatacc ttccgtgcgc ggcggcttgc cgatacgcgt catggtatcc gttgtcttgt
     1981 agcccaccca gcctggagga gcattttctc ggcgcatcgg aggattgttc acatcgtagt
     2041 cgtcctgaga tgtgtcgggg atcgtcgttt gtgccaagag gaatggttgg gtgagtgtcg
     2101 tgtgagccaa cacgacgcgc gcttgatgaa accggtccat cgggctactg ctcttg
//
LOCUS       DASTWE010001177         1291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1518214, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001177 DASTWE010000000
VERSION     DASTWE010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518214"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..469)
                     /locus_tag="VFT30_12260"
     CDS             complement(110..469)
                     /locus_tag="VFT30_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093454.1"
                     /translation="MGPTKVLVKDGGLYDAVSGKLVKDGFANRREMEDYVNHHYLVLP
                     AIDNAGRPWQLDGKPVYCLRGVQYETVDDQKVHLARCPECGGMGVRSDEFTVESDCIR
                     CTVCGNEFDTRLEMMET"
     gene            complement(569..>1291)
                     /locus_tag="VFT30_12265"
     CDS             complement(569..>1291)
                     /locus_tag="VFT30_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5093455.1"
                     /translation="SIERMRKQLAELATFLGKGVPTRNLEEFDLDTERLIGDLLGQAS
                     ELLHAYEYAELGEAAGLVNMTDEAPESAGMDSQRHSLLQRSRVLESCIAELEARRAAE
                     PKGAKARRQTLIGPQVVEHMTSEIQSVSQEASLREAGQMMQKWKLGSLLLTDDQSYVG
                     FITDSILAREVVANGVDANTTQVKACMRKPVVAIAGDRPIIDAVRLMKEQATRHVAVT
                     QDGQIIGVISVSNILRYYSGVV"
BASE COUNT          264 a          395 c          320 g          312 t
ORIGIN      
        1 gattacgatc tcatcgaccg ccagcacttc cagatttttg cgaaggcgga gggttgagag
       61 ccggtgattc tggggttcat gaatagagat ataactaagc aatcctttct tatgtctcca
      121 tcatttcgag ccgcgtatcg aactcgtttc cgcagactgt gcaccggatg caatccgatt
      181 ccaccgtaaa ttcgtcggaa cgtaccccca tgccgccgca ctccgggcag cgggcgaggt
      241 gaactttctg atcgtccacc gtctcgtatt gcacaccgcg aaggcagtat accggttttc
      301 cgtcaagctg ccatggtcgt cctgcattgt cgatggccgg taagacgagg taatgatggt
      361 tcacgtagtc ctccatctcg cggcgattcg caaagccgtc tttgaccagt ttgccggata
      421 cagcatcgta tagcccgccg tcttttacga gcactttggt cggtcccatc gcttcacctc
      481 cccgcgaaaa tcatacccgt cgcagaggca ccaaaatccc cctgcgttat ccgatctcat
      541 tgcatttcag acacagcaag ttccattgtt agacgacacc ggagtaatac cgaagaatat
      601 tggagactga gatgacaccg ataatctgtc cgtcttgcgt aacggcgacg tgcctggtag
      661 cttgctcctt cataagccgc acggcatcga taatcggacg atcgcctgca atggccacca
      721 ccggtttgcg catgcacgct ttcacctgcg tggtattcgc atcgacgccg ttggcgacca
      781 cttcgcgcgc caggatcgaa tccgtgatga accccacgta cgattggtcg tcggtcagaa
      841 ggagcgaccc taatttccat ttctgcatca tctgtcccgc ctcccgtaga ctggcttctt
      901 gcgagacact ctggatctcg gaagtcatgt gctccaccac ttgaggaccg atcagcgtct
      961 gccgacgggc cttcgctcct ttcggttcgg cagcccgacg tgcttccaat tccgcaatgc
     1021 agctttctaa cacccgactc cgctggagta agctgtgtcg ttgactgtcc ataccggcgc
     1081 tttccggtgc ttcgtccgtc atgttcacaa ggcccgccgc ttcacctagt tcagcatact
     1141 cataggcgtg gagaagctca gaggcctggc cgaggagatc accgatcaat cgctccgtgt
     1201 ccagatcaaa ctcttccaaa ttacgggtcg gtacgccctt ccccaggaat gtggccagct
     1261 cagccagctg tttgcgcatg cgttcgatac t
//
LOCUS       DASTWE010001178         1397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1291787, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001178 DASTWE010000000
VERSION     DASTWE010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291787"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFT30_12270"
     CDS             complement(<1..1085)
                     /locus_tag="VFT30_12270"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5093456.1"
                     /translation="MYPYGLIGNCQISALISAQGSIDWLCLPRPDSPPIFGKILDLEG
                     GQFSIISSVSSSETTTTQHYLPNTNILVTTVSLPNGEAFQITDFCPRFLQYGRIYRPA
                     ALFRIVEPLSGSPSIRVSCKPVSGWDKTPVRFVRGSSHLRYETRGEYLRLLTNMPLTY
                     LCEETPVVLTSKMYFALTWGIGIEDDLVKVSHEFLDETAKYWRSWVKHCSIPVVYQEE
                     VIRSALALKLHCYEDTGAILAAITTSLPEEPGGPRNWDYRYCWLRDAHFSLSAFYNLG
                     HFEEMEGFLKFLLNVGYAYEHSHERLAPVYTLSQDLPIPETEHVNWQGFLGSRPIRSH
                     NQAAEHIQNDVYGEMILTLAPIFFDNR"
     gene            1134..>1397
                     /locus_tag="VFT30_12275"
     CDS             1134..>1397
                     /locus_tag="VFT30_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU5093457.1"
                     /translation="MRLLTLSLRFVLPLAIVLGVIAYGVIPLVDSLELKWFVRDLDMR
                     SKTMVNTMEGPLADLLVSNSKGKIQAYFTRILQDERLYALGFCD"
BASE COUNT          322 a          341 c          407 g          327 t
ORIGIN      
        1 aatcggttgt cgaagaagat cggagcgaga gtgaggatca tctcgccata gacatcgttt
       61 tgtatatgtt cggcggcctg attgtgactc cgaatgggtc ggctgccgag gaagccctgc
      121 cagttgacat gttcggtttc cggaatcggc aggtcttggc tgagtgtgta aaccggagcc
      181 aagcgctcgt gagagtgctc gtaggcatag cccacgttga gtagaaactt caggaagcct
      241 tccatttctt cgaagtgacc gagattgtag aacgcggaaa gtgaaaagtg ggcatcgcgc
      301 aaccaacaat agcgatagtc ccagttgcga ggaccgccgg gttcttcggg caggctggtg
      361 gtgatggccg ccagaatcgc tccggtgtct tcataacagt gaagtttgag tgcaagggcc
      421 gaacggatga cctcttcctg atagacgacg ggaatcgaac aatgtttcac ccagcttcgc
      481 cagtacttgg cggtctcgtc cagaaattcg tggctgacct tgacgaggtc atcttcaatt
      541 ccgattcccc atgtcaacgc gaagtacatc ttcgaggtca gcacgaccgg tgtctcttcg
      601 cagaggtagg taagcggcat attggtgagc agccgcaggt attctcctcg cgtctcgtat
      661 cgaaggtggc tgctgccgcg gacaaatcgg accggagtct tgtcccagcc ggaaaccggc
      721 ttacagctga cgcgaatcga gggtgagccg ctcagcggct ctacgattcg aaacaaagca
      781 gcgggacgat agatgcgacc atattgcagg aagcgagggc aaaaatcggt gatttggaac
      841 gcctcaccgt tgggaagtga gacggtcgtc accaagatat tagtgttcgg aagatagtgc
      901 tgcgtcgtgg tcgtttccga ggacgagacg gacgagatga tggaaaactg gccaccctcc
      961 aaatccagta ttttcccgaa gatcggaggg ctatccggtc gtggaagaca cagccagtcg
     1021 atagaccctt gcgcgctgat cagcgctgaa atttgacaat taccgatgag tccgtacggg
     1081 tacataaggc gaccatattt gatttctccg tgaaagtaaa ctaacttggt cctatgagac
     1141 tgcttaccct ctcgcttcga tttgtgcttc cattggcgat cgtgttggga gtgatcgcct
     1201 acggagtgat cccgctcgtc gactcgttgg agctgaaatg gttcgtccgc gatctcgaca
     1261 tgcggtccaa aaccatggtc aatacgatgg agggtcctct tgcggacctc ttggtatcca
     1321 attcaaaggg caagatccag gcttatttta cgcgcattct tcaggacgaa cgcttgtacg
     1381 ccttgggatt ttgtgat
//
LOCUS       DASTWE010001179         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_581416, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001179 DASTWE010000000
VERSION     DASTWE010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581416"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /locus_tag="VFT30_12280"
     CDS             complement(<1..1963)
                     /locus_tag="VFT30_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5093458.1"
                     /translation="MLSLAMVVLGLTSLQRLPVDLFPQIQVPVAFVGVIYKGAPPLDI
                     EQSVVYPIEKAVSSASNVEHVESFSKQGIGAVQIWFNWGADINVGQMEVMQRITQILN
                     SLPPGILQPFIVKFDVSNIPVSIVSVSSDGLDERALYDLAYNTIAPQIEQIANVAAAT
                     VEGGKIRQININLDPALLSARGLSILDVVKSVKASNLILPSGNIKAGNLDYNVFTNNQ
                     FRTVDPIQDVIVKVNQQGNPVRVRDIGTVTDSSDIQTNIVHADGAKSVFLRVNKQPIA
                     NTVEVVDALRAALPKMFGIPEGVKLGISFDQSLYIRQSIHNLIEQALHGSLLAAAVIL
                     IFLRNLTSTAIISVAIPLSMLVTFIVLYFTGQTLNVFTLGGLALGIGRLVDDSIVELE
                     NIQRHLNTASSRWDSILEAAREVAMPIFASTVTTVVVFLPMFFVVGVARLLLIPLTVT
                     IAIALFTSFLVSRTVTPALCYKFLRPEQEVRRTMPDWFAKLMDWSRRRYESLDRSYEE
                     SLRWVLGHRRIFIAMVLLIFVGSLTLVPMIGTEFLPVSDESQFRIVLRAPVGQRVEKT
                     EQQVSEVERVLRANIPATELETIVSTTGILSQGRASLFNPNTGPHTSSIQVYLASPDK
                     RTRTQVEIMNDVRPKIVKLFPGVSMYFDPG"
     gene            complement(2119..>3077)
                     /locus_tag="VFT30_12285"
     CDS             complement(2119..>3077)
                     /locus_tag="VFT30_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5093459.1"
                     /translation="GYIAKLHVDKGDFVRANQLLVEIDHTDYHHAVNQAKANLLAATA
                     KVSQQNAMVRNAKLTFDRMQTLIKDQFVSQQDLDNAQVNLDAARAAHESLQAQVNQME
                     VALAQAETNLAYSYIRAPFPGYIAERNLDTGAYVSSATASTSTMSRGIMSLHDINTVR
                     VLIEVVERDIPLVKIGQKAELRAEAYPAQSFEGTVTRIVQALNRATRTMTVEIDLPNR
                     DRRLKGGMFARVEVMVGTHHQALQIPIDAVSRLEDTQYVYVVREGKAQRVDVEIGAHS
                     GNHVEITKGLTGHEQIIVAGKDLVHDGTPVQIEPLNAVKRET"
BASE COUNT          709 a          785 c          875 g          708 t
ORIGIN      
        1 cgccgggatc gaaatacatc gagactccgg gaaataattt gacgatctta gggcgcacat
       61 cgttcatgat ctcgacttga gttctggtcc gcttgtccgg tgaggccaag tacacttgaa
      121 tggaggatgt gtggggcccc gtgttggggt tgaagagcga cgcacgacct tgcgacagga
      181 tgcccgtcgt ggagacgatc gtctccaatt cagtcgcggg aatgttggct cggagcaccc
      241 gttcgacttc cgagacctgc tgttcggtct tttcaacccg ttggccgact ggcgcccgca
      301 ggacgatgcg aaactgactc tcatcggaaa cgggcaggaa ctcggtcccg atcatcggaa
      361 ccaaagtgag cgatccgaca aagatcagta gcaccatggc gataaagatc cggcgatgtc
      421 ccagtaccca ccgcaatgac tcttcgtaac tcctatcgag ggactcgtat cggcgacgac
      481 tccaatccat cagtttggca aaccagtccg gcattgtccg ccgaacttcc tgctcaggtc
      541 tgaggaactt gtagcagagc gccggcgtca ccgtgcggga gactaggaaa gaagtgaaga
      601 gcgcaatcgc aatcgtaacg gtcaacggta tcagcaacag acgggcgaca ccgaccacga
      661 agaacatcgg gaggaagacc accaccgtcg tgacggtcga cgcaaagatc ggcatcgcga
      721 cttcacgagc ggcctcgagg atactatccc accggctgga agcagtattc aggtggcgct
      781 ggatgttctc caactccacg atcgagtcat caaccagccg gccaatcccg agcgcgagcc
      841 cgccgagcgt gaagacatta agggtttgac cggtaaaata aagaacgata aacgtcacga
      901 gcatcgagag cggaatggcc acggaaatga tcgcggtact cgtgaggttg cgcaagaaga
      961 taagaatcac ggctgcggcc aacagcgagc cgtgaagggc ttgttcaatc agattgtgga
     1021 tggattggcg gatgtagagg gactggtcaa acgaaatgcc gagttttacg ccttcgggga
     1081 tgccaaacat tttggggagg gccgcgcgga gggcatctac tacctcgacc gtgttggcga
     1141 tcggttgctt gttcacacga agaaacaccg atttggctcc atcggcatga acgatgtttg
     1201 tttggatatc ggatgagtct gtcacggtcc ctatgtcgcg cacgcggacc ggattgccct
     1261 gctgattcac cttcacgatg acgtcttgga tcggatccac tgttcgaaac tgattgttcg
     1321 taaatacatt gtagtcaagg ttgccggctt tgatattgcc ggaaggcaga atcaaattgg
     1381 aggctttgac ggatttcacg acgtcgagaa tggagagccc acgggcactg aggagcgcag
     1441 gatcaaggtt gatattgatc tgacgaatct tgcctccttc gacggttgcc gccgcaacat
     1501 tggcgatctg ctcaatctgt ggggcgatcg tgttgtaggc gagatcataa agtgcccgct
     1561 cgtccagccc atcgctcgat accgaaacga tcgaaaccgg tatgttggaa acgtcgaact
     1621 tgacgataaa gggctgcaga atgcctggcg ggagactatt caaaatctgg gtgatgcgtt
     1681 gcatcacttc catttgaccg acattaatgt cggcccccca gttgaaccag atctgaacgg
     1741 ctccaatacc ctgtttgctg aacgattcga catgttcgac attggaagcg gaactgacgg
     1801 ccttttcgat ggggtagacg acgctctgtt cgatgtcgag cggaggcgct cctttgtaaa
     1861 taacgccaac gaaggcgacc ggaacctgga tttgcgggaa gagatcaacc ggcaagcgct
     1921 gaagagacgt cagccctagt accaccattg caagcgatag catcaggatc ccgatgcgat
     1981 tgcgtaatgc aagtagagtc aaccacatat aaaacgatga aaggtgaggg gtgaaaagtg
     2041 caaggtgaac gaaaagacgt cagtgatggg tgcgtgggga ataagcagaa ataatggcac
     2101 tccttcgcgt ttcacgtctt acgtctcacg ttttacagcg ttcagaggtt cgatttgtac
     2161 gggtgtccca tcgtgcacca agtctttacc ggcgacgatg atctgttcat gccccgtcaa
     2221 acctttggtg atttctacat ggttgccgct atgagccccg atctcgacat ccactcgctg
     2281 ggctttcccc tctcggacga catagacgta ttgcgtatct tctagtcgac tgacggcatc
     2341 gatgggaatc tgcaacgcct ggtgatgggt tcccaccatg acttcgactc gggcaaacat
     2401 tccccctttt aatcggcgat ctctattagg caggtcaatt tccactgtca tggtgcgtgt
     2461 cgcgcgattt aaggcctgga cgatacgcgt gacggttcct tcgaacgatt gggctggata
     2521 cgcttcggca cgaagttcgg ctttttggcc tatcttcacc agcggaatgt ctcgctcgac
     2581 gacttcgatc agcacacgga ccgtattaat gtcgtgcagg ctcatgatgc cccgcgacat
     2641 tgtcgaggtg cttgccgtcg cgctgcttac ataggccccg gtatctaaat ttcgttctgc
     2701 gatgtaaccg ggaaatgggg ctcggatgta agagtaggcg agattagtct ccgcctgggc
     2761 cagtgccacc tccatctgat tgacctgtgc ttgcagggat tcgtgtgctg ctctggccgc
     2821 gtccaggttc acctgcgcat tgtccaggtc ttgctgtgag acgaattggt ccttgatgag
     2881 ggtctgcatg cgatcgaacg tcagcttggc gttacgaacc atcgcattct gttgtgagac
     2941 cttcgctgtg gctgctaaaa ggttggcttt tgcctgattg acggcatggt ggtagtcggt
     3001 atgatcgatt tcaacgagta actgatttgc cctgacaaaa tcacccttat cgacatgcag
     3061 tttggcgatg tatccgt
//
LOCUS       DASTWE010001180         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_871980, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001180 DASTWE010000000
VERSION     DASTWE010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871980"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFT30_12290"
     CDS             complement(<1..326)
                     /locus_tag="VFT30_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEU5093460.1"
                     /translation="MREFPLTLSRFIIQNQTSHQDATEEFSSLLTQIGLVGKLISQDL
                     RRAGLINILGTTGDMNVQGETVKKLDAIANDDFVKVFQHSGYVCALASEEMEKPISIP
                     GNWPQG"
     gene            complement(376..1212)
                     /locus_tag="VFT30_12295"
     CDS             complement(376..1212)
                     /locus_tag="VFT30_12295"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU5093461.1"
                     /translation="MLTSAPAWSFLLPRAAKACRYAVLVAVVVLSPYSVGSIEAEPLG
                     EAKRPFQVGERLTYEVSWLNLTAAIAVMEVARMDGPKDQALAKLVGTAQSTPIITKFF
                     PVDNRVESELDLDTLTPEHMTFRRREGKKKEDIEYVFHQKEGIVTAVRGGTTESLPIP
                     AGTQDIISCLYYTRTRLPPNPGASLTMNVYHDKKNRPVEVRVEAIETIEGAWGKAETV
                     RVLVIMPFHGLFMNQGNIRVWVTNDERKTPVRMKAKVVLGSIVADLVDGLAGIGSAEQ
                     KY"
     gene            complement(1206..>2234)
                     /locus_tag="VFT30_12300"
     CDS             complement(1206..>2234)
                     /locus_tag="VFT30_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU5093462.1"
                     /translation="RRVMEKGAFVSGRGHLSEHPIIPDYLAYIQQNFSHVQANRLHVV
                     IDSGNGAASLVAQQALELLGCKVTGLYCDLDGRFPNHHPDPTVLENLSDLMQAVKEHR
                     ADVGIGYDGDADRIGAVDEQGDVLWGDRLLVLYSRDILAVKPGSTIISEVKASQSLYD
                     DIAKHGGRAIMWKTGHSLMKAKMREEAAVLAGEMSGHMFFADRYFGYDDAVYASCRLV
                     EILAKARQPLSALVSDLPQSIVTPEIRVDLPDAVKFDVVERVRQRFTEYLKTKQGLGP
                     RKLALQNLITIDGVRAIFDEGWGLIRASNTQPALVLRFEATSSAHLNVIRALIEDELA
                     VAKRALGC"
BASE COUNT          504 a          658 c          560 g          512 t
ORIGIN      
        1 ttgccctgcg gccaattgcc gggaatcgag attggttttt ccatttcttc ggaggccaag
       61 gcgcagacat acccgctgtg ttgaaagact ttgacgaagt catcgttcgc gatggcgtcc
      121 agcttcttga ccgtttctcc ctgcacattc atgtcgccgg tcgttccaag aatgttgatc
      181 agtccggcgc gccgaagatc ttgcgaaatg agcttgccga ccagaccaat ctgggtcaac
      241 agactggaga attcttccgt cgcatcttga tgcgaggtct gattctgaat gataaatctg
      301 cttaaagtaa ggggaaattc tctcataacg acgctcagta taacgggttt gacccgtgcg
      361 aacaataatc tatgttcaat acttttgctc ggcagagccg attcccgcca agccgtcgac
      421 cagatctgcg acgatcgatc caagcacgac cttcgctttc atacgaacag gagtctttcg
      481 ttcatcattg gtgacccaca cacggatgtt gccctgattc ataaacaacc catggaaggg
      541 catgatcaca agcactcgga cggtctcggc ctttccccat gccccctcaa tggtctcgat
      601 agcctcgaca cgaacctcga ccggacgatt tttcttgtcg tgatacacgt tcattgtcaa
      661 cgaggcgccc ggattcggcg gcaaccgcgt gcgtgtatag tacaagcatg aaatgatatc
      721 ctgtgttccg gccgggatgg ggagcgactc cgtcgttcct cctctgacgg ccgtgactat
      781 cccctctttt tgatgaaaga catactcgat atcttctttc tttttacctt cgcgacggcg
      841 gaacgtcatg tgttccggag tcaaggtatc caaatccagt tcagattcca cacgattgtc
      901 gacagggaaa aacttggtga taatcggtgt tgattgtgcg gtccccacca gcttcgcgag
      961 ggcttgatcc ttcggcccat ccatgcgggc cacctccatc accgcaatgg ctgcagtaag
     1021 gttaagccag gaaacctcat acgtcaggcg ttctccaacc tgaaacggac gcttcgcttc
     1081 gcctagaggt tcagcttcaa tggatccgac agagtatggt gacagaacaa ccacagcgac
     1141 caggacggca taccgacacg ccttggcagc acggggaagc aagaaagacc aggcaggcgc
     1201 agatgtcagc acccgagcgc ccgcttcgcc accgcgagtt catcctcgat gagcgctcga
     1261 atgacgttga ggtgcgcgga agatgtcgct tcaaatctca gtactaaggc cggttgggtg
     1321 ttggaagccc tgataagccc ccacccttca tcgaatatcg cacggacgcc gtcgatcgtg
     1381 atcaggtttt ggagcgccag tttcctcggc ccgaggccct gtttggtttt caagtactcg
     1441 gtaaatctct ggcgaacccg ctcaacgaca tcgaacttga cggcatcagg aagatccact
     1501 cgtatctcgg gtgtgacgat cgactgaggc agatccgaca ctaaagccga aagcggctgt
     1561 cgggccttcg ccaaaatttc tacgagccgg cacgacgcat agacggcatc gtcgtacccg
     1621 aaataccgat ctgcgaaaaa catgtgcccg gacatttcac cggccaatac cgccgcctct
     1681 tccctcattt tcgccttcat cagcgaatga ccggtcttcc acatgatggc gcgcccgcca
     1741 tgcttggcga tatcatcgta gagactctgt gacgccttca cttccgagat gatggtgctg
     1801 cccggtttca ccgctaggat gtcacgcgag tagaggacta gcaagcggtc cccccacagc
     1861 acatcgccct gctcatcaac agcgccgatc cggtccgcat ccccgtcata cccgattccc
     1921 acatcggccc tatgctcctt caccgcctgc atgagatctg acaagttctc tagtacagtg
     1981 gggtccggat ggtgattggg aaagcgtccg tccaaatcgc agtataatcc cgttacctta
     2041 catcccaaca actcaagcgc ttgttgggca acaagggagg ccgcgccgtt cccgctatcg
     2101 atcacgacat gcagccggtt cgcttggaca tgagagaagt tttgctgaat gtaagccaga
     2161 tagtcaggaa tgatcggatg ctcggagaga tgccccctcc ccgatacgaa cgcacctttt
     2221 tccatcactc ttcg
//
LOCUS       DASTWE010001181         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_161178, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001181 DASTWE010000000
VERSION     DASTWE010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161178"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFT30_12305"
     CDS             complement(<1..296)
                     /locus_tag="VFT30_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093463.1"
                     /translation="MTKLVFLTGLLFVGSQAVELTSSSDHAWAESIPSQPTVQLKGNI
                     LRGREIFNGKGVCYYCHGIDGYIGRRPRLEADTAALIAKLNPPPSDLRNPETLHL"
     gene            complement(383..859)
                     /locus_tag="VFT30_12310"
     CDS             complement(383..859)
                     /locus_tag="VFT30_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU5093464.1"
                     /translation="MSEQTVTVFYEKDHDRLDELFKTFQTSKRVDFAKAKEAFKEFKV
                     GLQRHIVWEEELLFPVWEEKTGMIEDGPTPVMRFEHSQIKQLLDAIHEKVEGQNLDTD
                     QDEQALVNLLSSHNRKEERALYPAIDNVTSEEERATVFGNMNSIPEDRYNSCCSQH"
     gene            complement(933..1241)
                     /locus_tag="VFT30_12315"
     CDS             complement(933..1241)
                     /locus_tag="VFT30_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093465.1"
                     /translation="MSQEMWDILSYIRTLVPFNLSAGSTARTVYAILSFSATARYLRS
                     EIKGQARKTEEEVLERDMEVRAALDELIECWKQFVEDRRADVETDLQADKEANREKNR
                     "
     gene            complement(1328..1912)
                     /locus_tag="VFT30_12320"
     CDS             complement(1328..1912)
                     /locus_tag="VFT30_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEU5093466.1"
                     /translation="MSTRMWFAGGLSVVLCMLGIWGTGHAGKESAGSVALETVVDYIH
                     SVLEANRTFYTVHVVERIQRRGLIKSSEKWRSESALPLPAQFFQESASLAELTGSKVR
                     YRLISLNPINKQNGPRTEFEQKALEAVMARPEQPYKGLVKEEDGRYFQAVYADYAVSP
                     GCVTCHNADSRSAKRDYKLNDVLGGVLISIPAPE"
BASE COUNT          465 a          650 c          514 g          559 t
ORIGIN      
        1 agatggagag tctcgggatt ccgcagatcg gaagggggag gattcagctt cgcaatgagc
       61 gcggcagtgt cggcttccaa tcgaggtctt ctcccgatat acccatcgat gccatgacag
      121 tagtaacaca cacccttgcc gttgaaaatc tctcggcccc gcaagatatt gccctttagc
      181 tgaacggtcg gctgcgatgg aatggactcg gcccaggcat gatcggatga agaggtcagc
      241 tccactgcct ggctaccgac aaagagcagg ccggtcaaga acactagttt ggtcaataga
      301 ttgcatccga gcaacgttcg agatcttgat atcgtttcaa tcctcgcctt tcccaccgcc
      361 ttttccttct atgagatcat cgtcagtgct gactacaaca gctattgtac cggtcctctg
      421 gaatactgtt catgttgccg aagacggttg cgcgttcttc ctcactagtc acgttatcga
      481 ttgcgggata gagcgcccgc tcttccttcc tattatgaga actcagtagg tttaccagcg
      541 cctgctcgtc ctgatcggta tcgagattct gcccttccac cttttcgtga atcgcgtcaa
      601 gcagctgctt gatctggcta tgttcaaatc gcatcacggg tgtcggtcca tcttcgatca
      661 taccggtttt ctcttcccac accgggaaaa gcaactcctc ttcccacacg atatgcctct
      721 ggaggccgac tttgaattcc ttgaaggctt ccttggcctt ggcaaagtcc acgcgtttcg
      781 aagtttgaaa ggttttgaac agctcatcca gccggtcgtg atccttttcg tagaataccg
      841 taacagtctg ctcactcatc gcgttccctc cgtcgtgcct acacgagtct ccatcggacc
      901 tcttatcgca cacttaccgc cttggatcgc ttctatcgat ttttctccct gttcgcttct
      961 ttgtctgctt gcagatcggt ttcaacatcg gcacgacgat cctcgacgaa ctgtttccaa
     1021 cactcgatca gctcatcaag cgcggctcgc acctccatgt ctctctccag cacctcttct
     1081 tccgtctttc ttgcctggcc tttgatctca ctgcgcaaat atctcgccgt tgccgagaat
     1141 gacaggatag catagacagt gcgtgcagtg gagccagccg agaggttgaa cggaaccaac
     1201 gtacggatgt aacttaaaat atcccacatt tcctggctca actcatgacc actacctccc
     1261 acattagata atcagagatc gtgtatcacg gtgctttggg tctgttgctg cgagtacaag
     1321 cttcccatca ctcgggtgcc ggaattgaaa tcaggactcc gcccagcaca tcgttgagct
     1381 tatagtcacg cttcgcgcta cgggaatcgg cgttatgaca ggtcacgcaa ccgggtgaaa
     1441 cggcatagtc tgcatagacg gcttggaaat atctaccgtc ctcttccttg acgaggccct
     1501 tgtatggctg ttcgggtcgc gccatgaccg cttcgagtgc cttctgttca aactcggttc
     1561 gcgggccgtt ctgcttgttg atcgggttga ggctgatcaa ccgatagcgc actttgcttc
     1621 ccgtcagctc ggccaagctc gccgactcct gaaaaaattg cgccgggaga gggagcgcac
     1681 tttctgaacg ccacttctcc gacgatttga tcaagcctct cctctgtatc cgctcaacga
     1741 cgtggaccgt gtaaaatgtc ctgtttgcct caagcaccga atggatataa tcgacgaccg
     1801 tttccaatgc gacgctgccg gctgattctt ttccggcatg acccgttccc caaattccaa
     1861 gcatgcaaag cacgacgctc aatcctccgg caaaccacat ccttgttgac atgttccctc
     1921 cttcgactga gcctatgcgg tgtggaaact ctgcaatatg gacggccaag aacggcgatc
     1981 tccctttcct catggatggt ggacgtctct ttcgtgaaga tcacgttgca acctcgtggt
     2041 atccgtgacc cccacaacgt gcgagtccaa ccacattcga gcagctgacc tgaacgaccg
     2101 gggcatgcgc cctcaaccgg ccgcatcttc aatggacacg ccaagcccaa tccaggctct
     2161 caaaagatca tggcgagtga ttgagtac
//
LOCUS       DASTWE010001182         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_225500, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001182 DASTWE010000000
VERSION     DASTWE010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225500"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFT30_12325"
     CDS             complement(<1..343)
                     /locus_tag="VFT30_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5093467.1"
                     /translation="MTLSIRLLLPVITVLSVIFATGERHVAYAVEPIAQPSLVLRDLI
                     HETLERNPELVAARKQWEASSQRIVQARSLDDPTLSVHLWNFPQTFNVTRADNSIFGL
                     SQNFPFPGKLAL"
     gene            complement(996..>1786)
                     /locus_tag="VFT30_12330"
     CDS             complement(996..>1786)
                     /locus_tag="VFT30_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5093468.1"
                     /translation="RDKEIVPAKTQNFRSLTGKGVMGTVEGRVVAVGTVLFLNELNVQ
                     TEPLLAQAESLRKEGQTVMFAAIDDKPAGLLGVADPVKSTTSEAIDLLHREGLRLVML
                     TGDNRTTAESVARRLQIDEVQADVLPEQKAAVIKRFQAEGHVVAMAGDGINDAPALAQ
                     AQVGIAMGTGTDVAMESAGVTLVKGDLRAIARARRLSRGTMRNIRQNLFFAFIYNALG
                     VPIAAGVLYPFVGVLLSPMIASAAMTFSSVSVIANALRLRRLNL"
BASE COUNT          421 a          502 c          500 g          363 t
ORIGIN      
        1 tcagagccaa tttgccgggg aacgggaaat tctgtgagag accgaagatg ctgttgtctg
       61 cccgcgtgac attgaaggtc tgcggaaaat tccacaagtg cacggacagg gtgggatcgt
      121 ccagtgaccg tgcttgtaca atccgttgcg aggaagcttc ccattgcttg cgtgctgcga
      181 caagctcggg atttctctca agagtctcat ggatcaagtc ccgcaacacc aaggatggtt
      241 gtgcaatcgg ctcgacagca taggcgacat gacgctcacc tgtggcgaag atcacgctca
      301 gaactgttat gaccggcaac aaaagccgaa ttgaaagagt catgtgatag tcctccccca
      361 agagcacttc cgcctgcgcc tgtcgtaatc tcagcccgtc ttgctgtaga aaagcgatcg
      421 gatggaccga ggctcggcgc cggacagata acgcgatggt gaaacggtat aaggaagaac
      481 cggaaagatc agatacgaag cactgtgctt gcgagagact gatgcggagg agaactcgat
      541 tcgagagagt gatatgggct ggaaattcgt acgagccgag ggctcaccgt tggaagcgta
      601 ggtaacgcat ggtctgccga ccaatgatgg tccacgagca acgatgacga ctgagcctgg
      661 gattcgactc ccaagatctt gaaggcgtca cataattgtc gactaggttg ttcgacagga
      721 gttgaacaac cgttggccat tgacgcatcg gtggtccctg acagcggcaa gagacaggca
      781 tatgcgttga aactgaacgc cagcaagaac accacgataa tcgcggcgag aaaagatcgg
      841 aagtgatgag aacttaatcg catggtgtga gtctatccgt ctgtcaactt aaccgtcaac
      901 ggtatttctc atacgtgggc ttcagtctct atcagcatag ctttccagta gccttctaca
      961 aaggcatgag ccgttccacg gcaactcacg aaacgttaca ggttgagccg gcgcaaacgg
     1021 agcgcgttcg cgatgaccga cacggaactg aacgtcatgg cggcgcttgc gatcatgggg
     1081 ctcaaaagga ccccgacgaa tggatagaga accccggctg cgataggcac acccagtgca
     1141 ttgtaaatga aggcgaaaaa cagattctgc cggatgttcc gcatcgtccc gcggctcaac
     1201 cggcgcgccc gggcgatggc gcggagatct cccttcacta gcgtgacacc ggcgctctcc
     1261 atcgcgacat ccgttcccgt ccccatggca attcctacct gtgcctgcgc caaagccggc
     1321 gcatcgttaa tgccgtctcc cgccatggcc accacgtgcc cttcggcctg gaaccgcttg
     1381 atgaccgccg ccttttgctc gggcaagacg tcggcctgga cttcatcgat ctggagccgt
     1441 cgggccacag actcggctgt tgtccgattg tcgccggtca gcatcacgag ccgtagtcct
     1501 tcgcgatgca acaaatcgat ggcctccgac gtggtggact taaccggatc agccacacct
     1561 aacaagcctg caggcttgtc gtcgatcgcc gcgaacatga cggtctgtcc ttccttccgc
     1621 aacgactcgg cctgagccaa taacggctcg gtttggacat tcagttcgtt gagaaacagg
     1681 actgtgccca cggcgaccac acggccctca accgtgccca tgactccttt tcctgtaagc
     1741 gaacgaaagt tctgggtttt cgccggaacg atctccttgt ctcgag
//
LOCUS       DASTWE010001183         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_807352, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001183 DASTWE010000000
VERSION     DASTWE010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807352"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1035
                     /gene="tal"
                     /locus_tag="VFT30_12335"
     CDS             <1..>1035
                     /gene="tal"
                     /locus_tag="VFT30_12335"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU5093469.1"
                     /translation="DNPLEQLLRCGQSYWLDNLTQHMIAGGELKGWIQDHGLRGITAN
                     PTTFREALTSHDYDRHIESLTYEGRSTKEIYETLLIHDVQQACDLLYDVYAETDGRDG
                     YVSLEVSPHMAYDTEETLHEARKYVKHVNRPNLLVKVPATDAGIPAIEQLLFEGIGVN
                     ITLLFSIPSYQAVIEAYISALERRVTAHQPLQTVVSVASFFVSRIDVLVDQLLQRRVP
                     PGNGGRQSLAAQLQGRAAVANAKLAYRTFRQVFGSDRWARLAVKGARVQRPLWASTGT
                     KNPNYRDVVYVESLVGRDTVNTMPETTLRAFADHGRVVPDAVETELEQADAVMQDLEK
                     TGINFNCVTW"
BASE COUNT          244 a          272 c          297 g          222 t
ORIGIN      
        1 cgataatcca ttagaacagc ttctgaggtg cggacaaagc tactggttgg acaatctcac
       61 ccaacacatg atcgccggcg gcgagctaaa gggatggatt caagaccatg gacttcgggg
      121 aattacggca aatcctacga catttcgtga ggcacttacc agccatgact atgaccggca
      181 tattgaatcc ctcacatacg aagggcggtc cacgaaggag atttacgaaa ccctattgat
      241 ccacgatgtg cagcaggcct gcgatttact gtatgacgtc tatgctgaaa cagatggacg
      301 ggacggctat gtcagcctgg aagtatcgcc gcatatggcg tacgacacgg aggagactct
      361 tcatgaagca cgcaaatatg tgaaacacgt gaatcgtccg aatctgctcg tgaaggtacc
      421 tgcgactgac gccgggatac cggcgatcga acagctgctt tttgagggta tcggagtcaa
      481 catcaccctg ttgttttcga ttcccagcta tcaggccgtc atcgaagcct acatttcggc
      541 tctcgaacgg cgcgtcacgg cccatcaacc gctgcagacg gtggtgtcgg tagcaagctt
      601 tttcgtgagc cgaatcgacg ttctggtcga tcagttattg cagcgacgcg ttcctccagg
      661 taacggcggt cgacaatcgc ttgcggccca attgcagggc cgcgccgcgg ttgccaatgc
      721 caagctggcc taccggacgt ttcgacaagt gtttggcagt gatcgatggg ctcgactggc
      781 agtaaagggc gcgcgtgtac agcgtccttt atgggccagc accgggacga agaatccgaa
      841 ctatcgggat gtcgtgtatg tcgaatcctt ggtgggacga gacaccgtca ataccatgcc
      901 tgagacgacg ctgcgggcct ttgctgatca tggccgggtc gtgcctgatg ccgtcgaaac
      961 cgagttggag caagcggatg ccgtcatgca agatctggaa aagacgggca tcaacttcaa
     1021 ttgcgtcacc tggca
//
LOCUS       DASTWE010001184         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_839701, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001184 DASTWE010000000
VERSION     DASTWE010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839701"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFT30_12340"
     CDS             complement(<1..612)
                     /locus_tag="VFT30_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093470.1"
                     /translation="MALKLQAKWLLLGVLIGPLPLWADADLDYQNRGDRFEGVKPKPV
                     SGYDIEVISVLADYRDPAERLPDQLRVGFYLQRPTPVHLTIREQDYRLYYWLDRVKPA
                     KEWQAKSVNEYTWSTATVLQQLDQKLNPYELGVLIRLGRETPAENEEIAPAILYHAQP
                     PEKISGYLFTMKANGDARLFCKIFREKEPTELMTQVFRRIHGGR"
     gene            complement(746..1768)
                     /locus_tag="VFT30_12345"
     CDS             complement(746..1768)
                     /locus_tag="VFT30_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEU5093471.1"
                     /translation="MALSYHIAVVEKKSKRAPLPKGVTRRRKPVGASTGLAAVELQAA
                     APPGVVAELHQLIETDGGKVLSVYRDPYGGQWLVQAALPIDLVEPTPYQRNLSDTHVR
                     KLEGVIGKIGRFLDPIIAVRITKPDHAAKYWIPNGNHRLSAMRTLGAKSIIAIVVPEA
                     AAAYQILALNTEKAHNLREKALEVIRMYRELAQFDHATENTYALEFEEPALITLGLCY
                     EERPRFSGGAYHPVLKRVDEFLQKPLHATLSVRQQRAKTVLALDDLIVKQVEALKAKG
                     LTSPYLKSFVVARVNPIRFRPKDAPALSFDDALDRMTQAAQKFNPDKIKTDDLAKSGG
                     VPDEVE"
     gene            1767..>2209
                     /locus_tag="VFT30_12350"
     CDS             1767..>2209
                     /locus_tag="VFT30_12350"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU5093472.1"
                     /translation="MVKESPNRSVLRIGHRGACGHAPENTVASIEQAIALRCIFAEVD
                     IQRTSDDELILLHDKQVDRTTNGRGCVSQLTLSDIQKLDAGGGQSPPILEDVLKAASG
                     RIGLILELKTGGLAYDVCATVRASGFNRPVIYASSLQEELQHVRRA"
BASE COUNT          476 a          619 c          611 g          503 t
ORIGIN      
        1 ccgccccccg tgaatccggc ggaacacttg cgtcatgagc tcggttggtt ctttttctcg
       61 aaatattttg cagaagagcc gcgcatcgcc gttggccttc atggtgaaca aatagccact
      121 tattttttct ggaggctgtg catgatagag gattgccggt gcaatctcct cgttctcagc
      181 cggtgtttct cttcccaggc gaatcaatac ccccaattca tatgggttaa gtttctgatc
      241 caattgttgc agcaccgtcg ctgtcgacca ggtatattca ttcaccgact tggcttgcca
      301 ttctttcgcc ggtttcaccc ggtcgagcca atagtacagc cggtaatcct gctcgcgaat
      361 ggtgagatgc actggggtag ggcgctgtaa atagaatccg acacggagtt gatctggcag
      421 acgttcggca gggtcacgat agtcggccaa aacggagatg acttcaatgt cgtagccgga
      481 gaccggtttc ggtttgactc cttcgaagcg gtcgccacgg ttttggtaat ccaggtcggc
      541 atcggcccac aatggaagag ggccaatcag cacgcccaag agaagccact tcgcctgaag
      601 cttgagcgcc atgagggatc cgcggaatgc cgaccaatct actgagccgt ccccgaagtt
      661 tggcgggagt atagcccaga ggcttaagtg aagctagcta tgtagggacc gcgaaaacgg
      721 agagaggatg cgggtaggaa accgactact ccacctcatc tggcactccg ccggatttgg
      781 ccagatcatc cgttttaatt ttgtcgggat tgaacttctg cgcggcctgc gtcatgcgat
      841 cgagtgcgtc atcgaacgac aacgccggcg catctttggg acggaaccga atcgggttca
      901 cacgggcgac gacgaagctt ttaagatagg gactggtcag ccctttagct ttaagggctt
      961 cgacttgttt gacaatgagg tcatccagcg ccagaacggt cttggcccgc tgctgacgaa
     1021 cactcagcgt agcatgcaac ggtttctgta gaaattcgtc cactcgtttc agtactggat
     1081 ggtacgctcc cccgctgaac cgaggccgtt cttcatagca gagtcccagt gtgatcagcg
     1141 ccggttcctc gaactccaag gcataagtgt tttccgttgc gtgatcgaat tgtgccagct
     1201 cccggtacat ccggatcacc tccaacgcct tctcacggag attgtgcgct ttctccgtat
     1261 tcagcgcgag aatctgatag gcggcagcag cttctggaac cacgatcgcg atgatgctct
     1321 tggcccccag cgtccgcatg gcggacagac gatggttgcc gttcggtatc caatacttcg
     1381 ctgcatggtc gggtttggtg attcgcacgg caataatggg atcgagaaag cgaccgattt
     1441 tcccgatgac cccttccaac tttcggacat gcgtgtcaga gagatttcgc tgatagggtg
     1501 tcggctcaac gaggtcgatc ggcaaagcgg cctggaccaa ccactgcccc ccataggggt
     1561 cacgatagac cgaaagaact ttccctccat ccgtctcgat cagctgatgc aattcggcca
     1621 cgacaccagg cggtgcagcg gcttgtaact ccaccgcggc cagaccggtg gaagctccga
     1681 ccggtttccg acgccgtgtc acccccttgg gcaacggtgc tcttttcgat ttcttttcaa
     1741 ccacggcgat atggtaggac agagccatgg taaaagaaag tcctaaccga agtgtccttc
     1801 ggatcggtca tcgcggcgcc tgcgggcacg caccggaaaa taccgtggcg tccatcgaac
     1861 aagcgatcgc actccgatgc atcttcgctg aagtggatat tcaacgaact tccgacgatg
     1921 agctgatact cttgcatgat aagcaggtag accgcaccac aaatggccgc gggtgcgttt
     1981 ctcagctgac tctttcagat atccagaagc tcgatgccgg tggcggtcag agccctccga
     2041 tcttagaaga cgtgctcaaa gccgcgagcg gtcgaatcgg cctgattctc gagctgaaga
     2101 ccgggggatt ggcctacgat gtctgcgcga ccgtccgtgc cagcggtttc aatcgacctg
     2161 tgatctatgc ctcgtcccta caggaagagc tacaacatgt ccgacgggc
//
LOCUS       DASTWE010001185         1376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_96444, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001185 DASTWE010000000
VERSION     DASTWE010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96444"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1376
                     /locus_tag="VFT30_12355"
     CDS             <1..>1376
                     /locus_tag="VFT30_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5093473.1"
                     /translation="LEPELTGLIATYAERPWPTVYERELAVIVDRIRAGFGAWYEAFP
                     RSCASQPGRHGTFHDCEARLPYIAGMGFDVLYLPPIHPIGRTFRKGRNNDPAGSPDSV
                     GSPWAIGAREGGHMAIHPDLGTPEDFRRLIGKAQEQGMEVALDLAFQCSPDHPYVKQH
                     PEWFVQRPDGTIQYAENPPKKYQDIYPLNFESDQWASLWRELKEIVMHWIDQGVRIFR
                     VDNPHTKPFAFWQWLIAEVKRDYPDVLFLSEAFTRPKVMYRLAKLGFTQSYTYFAWRQ
                     TKWELIQYFTELTKTDAREYFRPNLWPNTPDILTEQLQHGGRPTFMSRLILAATLGAN
                     YGIYGPAFELMESRPQKAGSEEYLDSEKYEIRTWDRERADSLKELICRVNRIRRENAA
                     FHNDANLTFHQVDNEELIAYSKSSVDGENVILTVVNLSPHYVHSGWMHLDLHALGVQA
                     DKPFQVVD"
BASE COUNT          319 a          347 c          387 g          323 t
ORIGIN      
        1 ctcgagcccg agttgaccgg tttgatagca acctatgcgg agcgtccctg gccgactgtg
       61 tatgagaggg aattggcggt cattgtggat cggatcaggg cgggattcgg tgcctggtac
      121 gaagcgtttc cgcgttcatg cgcgtcgcag ccaggccgtc atggtacctt tcacgattgt
      181 gaagctcgcc ttccctacat tgccgggatg ggtttcgatg tcttgtatct tcctccgatt
      241 catccgatcg gccgaacctt ccgaaagggc aggaataatg atccggctgg aagtcctgat
      301 tcagtgggaa gtccgtgggc catcggggcg cgggagggag gccatatggc aattcatccc
      361 gatctcggta cgccagaaga tttcaggaga ctcatcggca aggctcaaga gcaaggcatg
      421 gaggtcgcgc tcgatttggc ctttcaatgt tcaccggatc acccctacgt caagcaacat
      481 cctgaatggt tcgtgcagcg cccggacggc accattcaat acgccgaaaa tcctccgaag
      541 aagtatcagg acatctatcc gttgaatttt gaaagcgatc agtgggcttc cttatggcgg
      601 gaactaaaag agatcgtgat gcattggatc gatcaaggtg tcaggatttt ccgggtcgat
      661 aatcctcaca ccaagccgtt tgcattctgg caatggctga tcgcagaggt gaaacgcgac
      721 tatccagacg tgttgttcct ttcggaggcc ttcacacgac cgaaagtcat gtaccgcctg
      781 gcgaagctgg gtttcaccca gtcctatacc tattttgcat ggcggcagac caagtgggaa
      841 ttgatccagt acttcacgga attgaccaag acggatgcgc gggaatactt tcggcccaat
      901 ctttggccca ataccccgga tatcttgacg gagcaattgc agcacggcgg acgtcccact
      961 tttatgtccc gcctcatctt agctgccact ctgggggcta attatggaat ctatggtcct
     1021 gcgttcgagc tcatggaatc acgtccgcaa aaggccggca gcgaggagta tctcgattcg
     1081 gaaaagtatg aaatccgcac atgggatcgg gagcgagccg acagcctcaa ggagttgatc
     1141 tgccgggtta atcgcatccg ccgagagaat gccgcatttc ataatgatgc gaatctgacg
     1201 tttcatcagg tggacaatga agagctgatt gcatacagca agagttcggt cgatggagaa
     1261 aatgtgatac tgacggtagt aaacctcagc cctcactatg tccattcagg atggatgcat
     1321 ctggatctcc atgcgctcgg ggtgcaagcg gataaaccgt tccaggttgt agattt
//
LOCUS       DASTWE010001186         1364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_128869, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001186 DASTWE010000000
VERSION     DASTWE010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128869"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFT30_12360"
     CDS             <1..290
                     /locus_tag="VFT30_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar synthesis regulator FleN"
                     /protein_id="HEU5093474.1"
                     /translation="QAKSPREAAEVFSKLDVAVDHFLHVAVELVGAIPYDDYVHLAVM
                     QQKALSELFPDAPAAQAFKRLAQQILQWPKPGLPKSSVQLVWQRATTPAGN"
     gene            315..1064
                     /locus_tag="VFT30_12365"
     CDS             315..1064
                     /locus_tag="VFT30_12365"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEU5093475.1"
                     /translation="MSKTALPHERKSFSAQGVRREQLIKEFAHVIRAMAHRLAFRLPA
                     YLDAEDLISVGTIGLMDAMDKYDPTREAKFKTYAEFRIRGAMLDEIRSMDWIPRSVHE
                     RIGLLQKTHTALMSRLGRPPQDEEVATELKMSLEELDDFITRARGAVMISVDDLNLHE
                     PDGHKVVTMLADTHTPDPLSTLVNERERASLAGAIQSLPEKERLVLTLYYYEELTMKE
                     IGELLKVTESRVCQIHTKAIIRLKAHLQKPN"
BASE COUNT          345 a          342 c          369 g          308 t
ORIGIN      
        1 atcaggccaa gagtccccgt gaagctgccg aagtgttcag taagttggat gtggcggtgg
       61 atcattttct tcatgtggcc gtcgaattgg tcggggctat tccctatgac gactatgtgc
      121 atttggcggt tatgcagcag aaggcgcttt ctgaattatt cccggatgcg ccggcagccc
      181 aagcattcaa acggttagcg caacaaatac tccagtggcc gaagccaggt ctcccaaaaa
      241 gttccgtgca actcgtttgg caaagagcga caacgccggc cggcaactga aacacgatcg
      301 tacatgggaa gatcatgagc aaaacagcct taccacacga acggaaatct ttttcggccc
      361 agggagtcag gcgcgaacaa cttatcaaag agtttgccca tgtcattcgc gcgatggccc
      421 atcgactggc cttccgtctc ccagcgtact tggacgcgga ggatctgatt tcggtgggca
      481 ccatcggact catggatgcc atggacaagt atgaccctac acgggaagcg aagttcaaga
      541 cgtatgcgga gttcagaatc cgtggagcca tgctcgacga gattcggtcg atggattgga
      601 tcccacgatc cgttcatgaa cgaatcggcc tgctccaaaa gacgcacacg gcgctcatga
      661 gccggttggg ccgaccgccg caggatgaag aggtggcgac agaactgaag atgtctctgg
      721 aggagctgga tgatttcatt acgcgggcca gaggggcggt gatgatcagt gtagacgatc
      781 taaatcttca tgaaccggac gggcacaaag tggtcacgat gttagccgat acgcacacgc
      841 ctgatccact gtccacattg gtcaacgagc gtgaacgtgc atcgctcgcc ggagccattc
      901 aatcgttgcc ggaaaaagaa cggctcgtcc tgacattgta ttactatgag gaactcacga
      961 tgaaggagat cggagagcta ttaaaagtca ctgagtcacg ggtctgtcaa atccatacca
     1021 aagccatcat ccggctcaaa gcgcacttgc aaaaaccgaa ttgatcgaat cggtggatcc
     1081 tgtcagctgc tcgtttgttt tggttgtgat gcaatttcgg gatgttgtgc gatcacccgc
     1141 cttcagttcc gagtcaaaga aaccgatacc tctctaggcg agatctcgac aagtgcctgg
     1201 tgtattgggt gggaaatcca tcgcatcagg tctgaacgtt gatcgagtct ccggtttatt
     1261 cccgattcaa gtcaaattct aaatcagcaa ttacgacaat gcatttgaaa atatcacacc
     1321 atgggctctc acgatcgcga catcgatctc ttgcatggtg gtgt
//
LOCUS       DASTWE010001187         1498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_645895, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001187 DASTWE010000000
VERSION     DASTWE010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645895"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..645
                     /locus_tag="VFT30_12370"
     CDS             112..645
                     /locus_tag="VFT30_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093476.1"
                     /translation="MVEEEVLTAAEEALSEEADVWSVDAERFDCGEVRLGEDEVRLDE
                     DEVRLDEDEDEVRLDEDEVRLVDDEVRVDEDEVILDDDEVRLDEEGRLDEDEGRLGEA
                     EVRLGEAEVRLGEDEVGLGGGEVPSVAAVLLNEDEVALGEDEVRSAAGEVLFFLFGAL
                     RFGEELRSLLRPVSLEN"
     gene            complement(1278..>1498)
                     /locus_tag="VFT30_12375"
     CDS             complement(1278..>1498)
                     /locus_tag="VFT30_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093477.1"
                     /translation="WCPVWSRACAMPQLFVRQRCLCIVAVFSLCFRADRELTGFPRLF
                     RVFFFLVLDHRKDPGQRSELCRVVFPVY"
BASE COUNT          352 a          346 c          487 g          313 t
ORIGIN      
        1 ggagaagtcg tccggtgcgt gtgagtgggt gacttcatcc tcctggtttc cgatctatcc
       61 gtggaagagg aggatctgtg ggaggtcctg tgagatgctg aagaggactg cttggttgaa
      121 gaagaagtct taacggcagc cgaagaagcc ctttctgaag aagctgacgt ctggtcggtt
      181 gatgcggagc gattcgactg cggagaagtc cgcttgggcg aagacgaagt ccgtctggac
      241 gaggacgagg tccgtttgga tgaggacgag gacgaggtcc gtttggatga ggacgaagtc
      301 cggttggttg atgacgaagt tcgagtggat gaggacgagg tcattctgga tgacgacgaa
      361 gtccgcttgg atgaagaagg ccgtttagac gaagacgaag gccgtttggg cgaagccgaa
      421 gttcgtctag gcgaagccga agtccgttta ggcgaagacg aagtcggcct tggcgggggc
      481 gaagtcccgt cggtcgcagc ggtcttactt aacgaagacg aagttgcgtt gggcgaagac
      541 gaagtccgat cagccgcggg agaagtgctc ttcttcctct tcggggcact tcggttcgga
      601 gaagaacttc gttccctttt gcggccagtt tcactggaga actgaagaac gatgtctcta
      661 atgggttgaa gttgggcaca ccaattatcg ttggccttct gtagggcagc gtcgaacatg
      721 gattggatgg cctccaacga caaattcggg tcgggaatga caggcaagga ggcgggaggg
      781 agagtggccg gagcgatgtc acctatcgtg tcgttgggaa gcggaggtgc ttgttcgccg
      841 gtcggcaaag cagtggtgag tccgagcttt tctagatccg tggaaaaggt atcatcgttg
      901 caaatctcca caaattctgt gccctcgatg atgtttgcta cttcctctgg aaggataagg
      961 tgagaaggca cttcctccaa gaaacccgta gcggtcgagc tggtggccga cgaagaccta
     1021 tcccgttccc gcttcgtctt atgttgcgtg tagcgtttag tggatcggtc ctgtcgaata
     1081 cggattcctt tccaatcgtc ttcagaaagg gaggcacaaa tgtcgcatgg ggccatcttg
     1141 gggtcacagt tcctacacga tcggcacaag gggtgtgggt cggtagcgat caaaagcacc
     1201 ccgcatttcg ggcgagtgca gtgacgtccc ttagccatgc tgcggccacg aggcgttagg
     1261 cctaacacgg gtctggttca atacacggga aaaacaacac ggcacaactc cgatcgttga
     1321 ccagggtcct tccggtgatc caaaacgagg aaaaaaaaaa ctcgaaacag gcggggaaat
     1381 ccggttaact cccgatcggc tcgaaaacag agagagaaca cggcgacgat gcaaaggcag
     1441 cgttgcctga caaacaactg aggcatcgcg cacgcgcgcg accagaccgg acaccagc
//
LOCUS       DASTWE010001188         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1486281, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001188 DASTWE010000000
VERSION     DASTWE010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486281"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFT30_12380"
     CDS             complement(<1..279)
                     /locus_tag="VFT30_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5093478.1"
                     /translation="MIKSLDGHHTEGEAGFTLTEVMIAAAMSTAILAAGFGALTVSQK
                     TTRITGQVSNTQAAARNALDMLAADIKLAGFGIQGMMGGAIGGCQVNGI"
     gene            complement(323..868)
                     /gene="pilV"
                     /locus_tag="VFT30_12385"
     CDS             complement(323..868)
                     /gene="pilV"
                     /locus_tag="VFT30_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEU5093479.1"
                     /translation="MKSRKEMGFTLVEVMVSATVLGLGVMGMAAMQGVAFTKNVDAND
                     LSIVTNVASDMVERIQNNRRYAWAYDNLDTVGAGNCLAGGMPAPFPATAPPFGGKRPS
                     NSAILQTMTQTVQGDCTQWRTLVLASNLVAARGTVQVAPIVPTSSNSGAVQVVVQVQW
                     NDRGPGQRPRAVVFRTQIEPE"
     gene            complement(888..1397)
                     /locus_tag="VFT30_12390"
     CDS             complement(888..1397)
                     /locus_tag="VFT30_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5093480.1"
                     /translation="MLCQRLTIVRSMTNQRGFTLLEIMIVVVIIGIITALAVPNFMTM
                     YARNELYQTTTSLYNRLVFARSAAISRNAMITATPGNPLPSGEKGMTFTTPLGIETFP
                     LNVTFVLPLPVQPIGFTPRGLSTTPLATQTIQLQSVRDPNLIYSISLAPSGKVAWCTR
                     QINPCLQNQ"
BASE COUNT          329 a          428 c          393 g          386 t
ORIGIN      
        1 gattccattg acttggcatc cacctattgc tcctcccatc atcccctgta tgccgaatcc
       61 ggcaagcttg atatcggctg cgagcatgtc caacgcattc ctggctgcgg cttgtgtgtt
      121 acttacttgg cccgtgattc gcgtcgtctt ttgactgaca gtcaaggcgc caaagccggc
      181 tgcaaggatc gccgtggaca tggctgcagc aatcatcacc tcggtgagtg tgaatccggc
      241 ttcaccctcg gtatgatgac catccaatga cttgatcatg aggatccccc attgatgcgc
      301 gacctttggt ctccaggagg ttctattcgg gctcgatctg tgttcggaat acgacggctc
      361 taggccgttg ccctggcccg cgatcgttcc attgcacttg cacaacaact tgaactgcgc
      421 cggaattgct tgaggtcggc acaatcggtg ccacttgcac tgttcctcga gcagcgacca
      481 ggttcgatgc gagtacgaga gttcgccact gcgtgcagtc tccttgcaca gtttgtgtca
      541 tcgtctgcaa aattgccgaa ttcgaaggcc gttttccccc gaagggaggg gctgtggcag
      601 ggaacggcgc tggcattcct cccgccaaac agttccccgc tcccaccgtg tcgagattgt
      661 cgtatgccca ggcatagcgg cgattatttt ggattcgttc gaccatgtcc gaagcaacgt
      721 tcgtaacgat ggagagatca ttggcatcaa cgttcttggt aaatgccaca ccttgcatgg
      781 cagccatccc cattactccc aatcccaaca ctgttgctga caccatgacc tccacgagag
      841 tgaaacccat ttccttacga ctcttcatcg catcacctgc tttctcacta ctggttctgt
      901 aagcaaggat tgatctgtct ggtacaccat gcgactttgc cggaaggagc caatgatatc
      961 gagtagatca agttggggtc acgcacgctc tgcagctgaa tcgtctgagt tgccaatggg
     1021 gtcgtgctca ggcctctcgg tgtaaatcca atgggctgca ccggcaatgg caaaacaaag
     1081 gtgacattga gcggaaacgt ttcaatgcca agaggggtgg taaatgtcat tcccttttcc
     1141 ccggagggga gtggatttcc aggtgtggcc gtgatcatcg cattgcgact aatagccgcc
     1201 gaccgtgcaa acactagccg gttatataaa ctcgtggtcg tctggtagag ctcgttgcga
     1261 gcatacattg tcatgaaatt gggtaccgct agcgcggtga taatcccaat aatcacaaca
     1321 acaatcatga tctccagcag ggtgaaccct cgctggttcg tcatggatcg cacaatggtt
     1381 agccgttgac acaacatggc gcctcctgat cgagcaaggg gcatgccgct ccaagcagga
     1441 cccgcattcc gtgtaacctc ttgatgtgct gacgtttctt ttcgccaggg atcattgcaa
     1501 tgcgttcgtt tttcgcactc atgtacgaca attttc
//
LOCUS       DASTWE010001189         1424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_807444, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001189 DASTWE010000000
VERSION     DASTWE010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807444"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFT30_12395"
     CDS             <1..503
                     /locus_tag="VFT30_12395"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEU5093481.1"
                     /translation="PDDPGLDGMAELWFRDKRHFETTIGREAPAEIMADRFNEYADFN
                     KGTWLTVTEHLNVDGPTTRETTKLVYFVKRREGIERSALDKYWLETHIPNVAAALRKT
                     PTARRYTVNLVDPARERIYDGIAQIWMDGPNPSFADVTSYEPDQFNEMVQPLLISRGH
                     EIRIVD"
     gene            568..>1424
                     /locus_tag="VFT30_12400"
     CDS             568..>1424
                     /locus_tag="VFT30_12400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5093482.1"
                     /translation="MSRIIDADSHFMEPLDLWPRYIEPRYRDRCVRFERAADTGRYVL
                     LIEDHRITNSGDLTIEELLGVAVGYGQKEAGKGLKSFDPAEAFNHSLEDMPARLRFLD
                     DEGIESQFIYPTLGLFWEDLVTDPDLAAAHARAYNTWMLEVCTGYRKRLYPVGHITLR
                     HPGEGVKELQRLAKADVKTVFVGALPIDGKSFGHRDFDPVWTAAQELDIAVGIHLVVH
                     SRYLGNEWYKDRDPGFMFLSMNVIQDPRMALTTMVYDGVFERFPRLRVATIESASGWV
                     AEWIDRLDYR"
BASE COUNT          333 a          369 c          397 g          325 t
ORIGIN      
        1 ctccggacga cccggggttg gacggcatgg ccgaattatg gtttcgcgac aagcggcatt
       61 tcgaaaccac gatcggtcgt gaggctccag cagaaatcat ggcggatcgt tttaatgagt
      121 acgccgactt caacaaaggt acctggttga ctgttaccga gcacctcaac gtcgacggtc
      181 caacgacacg cgagaccacc aagcttgttt actttgtcaa acgacgtgaa ggaatcgagc
      241 gctctgcttt ggacaaatac tggcttgaaa ctcatatccc aaatgtcgca gcggcactac
      301 gaaaaacgcc gaccgccaga cgatacaccg taaatttggt cgatcctgct cgcgaacgca
      361 tctacgacgg tattgcgcaa atctggatgg acggccccaa tcctagcttt gctgacgtca
      421 ccagctacga gccggaccag tttaatgaaa tggtccaacc tctgctgata tcacgcggcc
      481 acgagattcg tatcgtggat tgatcttgac gggtagtcgg taagggcccc ttaagttgag
      541 gatactcgag aaaaaggagg acacaccatg agccgcatta ttgacgctga cagccatttc
      601 atggagccgt tggatctgtg gcctcgctac attgagccgc gatatcgtga ccgctgcgta
      661 cgatttgagc gtgcggctga caccgggcgg tatgtcctct tgatcgaaga tcatcgtatc
      721 accaactcgg gcgacctcac gattgaagaa ctgctcggag ttgccgtggg ctacgggcag
      781 aaagaagcag gcaaaggcct caaatcgttt gaccccgccg aggcgttcaa tcattcactc
      841 gaggatatgc cggcgcggct tcgtttcctt gacgacgaag ggattgaaag tcagttcatc
      901 tatccgacgt tggggttgtt ctgggaagat ttagtgactg atcctgacct cgctgccgcg
      961 catgcacgtg cctataacac ctggatgtta gaggtttgca caggctatcg gaaacgattg
     1021 tatcccgtgg gtcacattac gttacgtcat cctggtgaag gggtaaaaga gctgcaacgg
     1081 ttagcgaaag ctgacgtgaa gaccgttttt gtcggggcgc tacctattga cggcaagagc
     1141 ttcggccatc gtgacttcga cccagtatgg acggcggcac aggaactcga catcgccgtc
     1201 ggcatccatc ttgttgttca ttctcgttac ctcggcaatg aatggtacaa agatcgagac
     1261 cctggcttta tgttccttag catgaacgtg atccaagacc cgcgtatggc actaacgacg
     1321 atggtctacg acggcgtgtt tgagcggttc ccacgcttac gagtggcgac gatcgaatct
     1381 gccagcggct gggttgccga gtggattgac cggctggatt atcg
//
LOCUS       DASTWE010001190         1433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_64249, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001190 DASTWE010000000
VERSION     DASTWE010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64249"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1433)
                     /locus_tag="VFT30_12405"
     CDS             complement(<1..>1433)
                     /locus_tag="VFT30_12405"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5093483.1"
                     /translation="VIQTTQTILLNAVDLVLQSATAEDTNQRKFNALIELEQKTQRAG
                     LSFQVPMQPGDWRLHLSFHGKLNDQLRGFYRSTYKDTSGTLETLAATQFEATDARRAF
                     PCWDEPDFKAVFATTLVIDPHLTAISNTSVTSESVESNKKLVRFADTIKMSTYLVAFV
                     VGRMEATEPVSVGKTPLRLWTVPGKQPLTPFGQDIAAASLKFFEDYYGMPYPGDKLDL
                     VAIPDFASGAMENLGAITFRETALLVDRQTGTHAELERVADVVAHENAHMWFGDLVTM
                     SWWNGLWLNEAFATFMEMLAVDAWKPEWKRWESFSVARAAAFSIDGLVSTRPIEYPVH
                     APKDADAMFDILTYEKGASVLRMLEQHIGSTVFRDGVRQYLRTHAYGNADTNDLWTAL
                     GTVARQPVPELMNGWIFQPGFPLVTAEVCNQELRLSQQRFTYLAQAATDELWQIPLQI
                     RLVIGDRTEHRRLLLTERETTIRVPAG"
BASE COUNT          307 a          409 c          398 g          319 t
ORIGIN      
        1 ccccagccgg tactctgata gtcgtctccc gctccgtcaa cagcagccga cgatgttccg
       61 tacgatcccc gattaccagc cgaatctgga gggggatctg ccagagctcg tcagtcgcgg
      121 cctgcgcgag ataagtgaat cgttgttgtg agagccgcaa ttcctgattg cacacctccg
      181 ctgtcaccaa agggaagccg ggttgaaaga tccagccgtt catcaactca ggtaccggtt
      241 gcctggcgac agtgccaagg gccgtccaca gatcattcgt atccgcgttg ccataagcat
      301 gggtccggag gtactgtcgc acaccgtctc ggaagaccgt ggagccgata tgttgctcca
      361 acatccgaag gaccgacgca cctttttcat aggtcaaaat gtcgaacatc gcatcggcat
      421 ctttcggtgc gtgaactggg tattcgatcg gtctggtgct caccaatcca tcgatcgaga
      481 aggctgccgc tcgcgcgacg ctgaaactct cccaacgttt ccattccggt ttccaggcgt
      541 cgacggccag catctccatg aaggtcgcga aggcctcgtt cagccagagc ccgttccacc
      601 aggacatggt gactaagtcg ccgaaccaca tgtgggcgtt ctcatgggct accacgtcgg
      661 ccacgcgctc gagctcagca tgggttccag tctgtcgatc cacgagtaac gccgtctcgc
      721 ggaaggtgat agcgccgaga ttttccatcg cccctgaagc aaagtccggg atcgccacga
      781 gatcgagctt gtctccgggg tatggcatgc cgtagtaatc ttcgaagaat ttcaacgatg
      841 ccgccgcaat atcctgtccg aacggtgtga gcggttgttt tcccgggacc gtccacagcc
      901 gaaggggtgt cttaccgacc gataccggct ccgtcgcctc catacgaccg accacgaagg
      961 ccacgagata ggtcgacatc ttgatggtgt ccgcaaatcg tactagtttc ttgttgctct
     1021 caacggattc cgatgtaacc gatgtgttgg aaatcgccgt gagatgaggg tcgatcacga
     1081 gcgtggtcgc gaagacggcc ttaaaatctg gttcgtccca acaaggaaag gctcgccgcg
     1141 catccgtagc ttcgaattgc gtggccgcca acgtctccaa ggttcctgaa gtatctttgt
     1201 atgtactacg atagaagccg cggagctgat cgttcaattt gccatgaaaa gaaagatgca
     1261 ggcgccagtc gccgggctgc atgggtactt gaaaagagag cccggcccgt tgagtcttct
     1321 gttccaattc gataagcgcg ttgaatttcc gctgattcgt gtcctcagcc gtggctgact
     1381 gaagtacgag atcgacggca ttcaatagga tggtttgcgt ggtctgtata acc
//
LOCUS       DASTWE010001191         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_969220, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001191 DASTWE010000000
VERSION     DASTWE010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969220"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFT30_12410"
     CDS             <1..324
                     /locus_tag="VFT30_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093484.1"
                     /translation="AVGKTLVEETKQFGLPYDKILRGPIPDPFIKGGNTRKVPFSLAL
                     PSGTAAASIEAVLTYALIPTPEPFLWERYLATLKTETEREESRKIIQEYTQRHFLTYR
                     VKSLS"
     gene            338..1579
                     /locus_tag="VFT30_12415"
     CDS             338..1579
                     /locus_tag="VFT30_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEU5093485.1"
                     /translation="MKPLPAMMAVGLSFVITICAAVLSDGALTSVSAQNVTSQSVIEK
                     TFPHSSKCKRCHERVYEEWETSPLAKSIHSPAFRASLDAYLNSPAGKDKALCFRCHAS
                     HVREFQEHVQLFIDQAKAGDPSLDGVACSQCHLIKHVDRAKHPPEPKYEMGGKTLYGP
                     YQDFVPNLAHQSMELSLFQKSDLCLNCHQSVPSASNLGKANDLLGNWDQSRAVKSGKE
                     CQSCHMPQQVGESANGEKKRKIANHTFPGRIGKLRQEAAKLDVQTKIDGDKTTVMVKV
                     QSLVPHNLPTTHPAWASVVLGLEIKGKNLKTVFSDKRVYGRTYLDAQGQKTVFDFEAT
                     KVAEDTVLKPEETREEIFTFPTPKDTKTFDVEVGLNYAPLNGPASFLQRVEAESSQGS
                     QDPVFQPIEIVKRTENVPVNK"
BASE COUNT          426 a          417 c          411 g          346 t
ORIGIN      
        1 gcggtgggca agacattggt ggaagaaacg aaacagtttg gattgcctta tgacaaaatc
       61 cttcgcggtc cgattcctga tcccttcatc aagggcggca acacgagaaa ggtccctttt
      121 tcgctggcgc ttccgtccgg cacagccgcc gcgtcgatag aggcagtgct cacctatgcg
      181 ctcattccaa cgcccgaacc tttcttatgg gaacgatacc ttgcgactct gaaaaccgaa
      241 acagagcggg aggagtcgag aaaaatcatt caggaatata cgcagcggca ctttctgact
      301 tatcgtgtca aatcactctc ctagagttac agtcatcgtg aaaccgttac ctgccatgat
      361 ggcggtcggt ctcagttttg tcataacgat ttgtgccgct gtgttatctg atggcgcgtt
      421 aacatcggtt tctgctcaga acgtcaccag tcagtcagtg attgaaaaga ccttccctca
      481 ttccagcaaa tgcaaacggt gtcacgaacg tgtgtacgaa gaatgggaaa cctcgcctct
      541 ggcgaagtcc atccattccc cggcgtttcg tgcgtctctc gatgcctatc tcaattcgcc
      601 ggctggaaag gacaaggcgc tctgtttccg ttgccacgcc tcgcatgtgc gagagttcca
      661 ggagcatgtt cagctcttta tcgatcaagc gaaagcggga gacccctcct tggacggagt
      721 tgcctgcagt caatgtcatc tgatcaagca tgtagatcga gccaaacacc cgccggaacc
      781 caaatatgag atggggggaa agacgctcta tgggccatac caggatttcg tcccaaacct
      841 ggctcatcaa tcgatggaac tgagcctgtt tcaaaagtcg gacctctgtc tgaactgcca
      901 tcagtccgtg ccatcagctt caaacctggg caaggccaac gatttgctcg gaaattggga
      961 ccagagccgg gctgtaaagt ccggtaaaga atgtcaaagt tgccatatgc cgcaacaagt
     1021 aggggagtcg gccaatgggg aaaagaaacg taaaatcgca aaccatacgt ttccagggcg
     1081 aatcggtaag ttgcgtcagg aagcagcgaa actagacgtg cagacaaaga tcgacggaga
     1141 caagacaacc gtcatggtaa aggtccaaag cctggtaccg cacaacctgc ctaccactca
     1201 tccggcttgg gcttcggtgg tattgggtct tgagatcaag ggtaagaacc tcaaaacggt
     1261 attttccgat aagcgtgtct acggtcgaac gtatctggat gcccaaggac agaagaccgt
     1321 gtttgacttc gaagcaacga aggtggccga agacacggtc ctaaaacccg aagaaaccag
     1381 agaagaaata ttcacgtttc cgaccccgaa agataccaag accttcgacg tcgaagtcgg
     1441 attgaattat gctccattga atggccccgc ctcattcctc cagcgcgttg aagctgagtc
     1501 atcccaaggc tcacaagatc cagtcttcca gccgatagag atcgtcaagc ggacggagaa
     1561 tgtgccggtc aataaatgac ataggacgtc tcttggaagc
//
LOCUS       DASTWE010001192         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1098558, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001192 DASTWE010000000
VERSION     DASTWE010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098558"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFT30_12420"
     CDS             complement(<1..205)
                     /locus_tag="VFT30_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEU5093486.1"
                     /translation="MGQRHFDDELAELKTKLARMAGLAEDQIDKALTALVTRDSALAC
                     QVIERDHKVNALDVEIDEACIELL"
     gene            complement(211..1011)
                     /gene="pstB"
                     /locus_tag="VFT30_12425"
     CDS             complement(211..1011)
                     /gene="pstB"
                     /locus_tag="VFT30_12425"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU5093487.1"
                     /translation="MDSRDSKVSDPRSPQVKPKLRVQGFNFFYGLVQSLFKIDLEISE
                     HQVTAFIGPSGCGKSTLLRCMNRLNDLIEGARHEGNILLDDVDIFNPKIDITDLRKRV
                     GMVFQKSNPFPKSIYENVAYGPRLQGLKSRTVLEDTVEGSLRGAGLWEEVKDRLHKSA
                     LGLSGGQQQRLCIARALAVKPEVLLMDEPCSALDPIATGIIEELLFSLKKELTVVIVT
                     HNMQQAARVSDSTAFMYLGQLIEVGRTKQLFTNPSKKQTEDYITGRFG"
     gene            complement(1070..>1265)
                     /locus_tag="VFT30_12430"
     CDS             complement(1070..>1265)
                     /locus_tag="VFT30_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HEU5093488.1"
                     /translation="HLDRKFMHLGFHIYDVGFQSPNVEAAKPMVYVTTLVLILVVVTL
                     NLTGVVLRNRMRKKYAGSAV"
BASE COUNT          289 a          358 c          316 g          302 t
ORIGIN      
        1 ccaacagctc gatgcaggct tcatcgattt ccacatctag tgcgttcacc ttgtgatctc
       61 gttcgatcac ctggcaggct agggccgagt cgcgtgtgac cagcgccgtc aatgctttgt
      121 cgatttggtc ctcggcgaga ccagccatcc gcgccagttt tgtcttaagc tccgcaagtt
      181 catcgtcaaa atgtcgctgt cccatacaga tcacccgaat cgtccagtga tatagtcttc
      241 cgtctgcttc ttcgaagggt tcgtgaacag ctgtttcgta cgaccgacct cgatcaattg
      301 cccgaggtac atgaacgccg tactatcgga cacccgcgcc gcttgctgca tattgtgcgt
      361 cacgatgacg accgtcaact ccttcttgag tgagaacagc aattcttcga tgatccccgt
      421 ggcaatcggg tcgagcgccg aacagggctc atccatcaga agaacctccg gcttgacagc
      481 gagggctcgc gcaatacaga gccgctgctg ctgaccgccg gacaaaccga gtgcgctctt
      541 gtggagtcgg tccttcactt cctcccagag gccggccccc cggagacttc cttccacagt
      601 atcttccaga accgtccggc tcttcagtcc ttgcagccgc gggccgtacg cgacattttc
      661 atagatcgat ttgggaaagg gattggattt ttggaacacc attcccacgc gcttacgcag
      721 atcggtgata tcgatcttgg ggttaaagat atcgacatcg tcaaggagga tattcccttc
      781 atgcctcgca ccttcgatga gatcattcaa gcggttcatg catcgaagca acgtggattt
      841 tccgcaaccg gatggtccaa tgaatgcagt cacttgatgt tcagaaatct ccaggtcgat
      901 cttgaagagc gattggacaa gcccgtagaa aaagttgaaa ccctgcaccc gaagcttggg
      961 ttttacttgg ggactgcgtg ggtccgacac cttggaatca cgggaatcca tgctctccat
     1021 ctccaataag gaagaagtag gtggactgtg agggataccg gacctcagct catactgctg
     1081 atccagcata tttcttcctc attcgatttc gcaagacgac gcccgtcaga ttaagcgtca
     1141 cgacgacaag aattagcacc aacgtggtca catagaccat gggcttggcg gcttcaacgt
     1201 tcggcgattg aaatcccacg tcatagatat gaaagcccaa gtgcataaac tttcggtcca
     1261 ggtgc
//
LOCUS       DASTWE010001193         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_193495, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001193 DASTWE010000000
VERSION     DASTWE010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193495"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          390 a          396 c          307 g          248 t
ORIGIN      
        1 ggctcgccaa gatcttccat tgccgtggtc gaaaaccggc agagtctgca ctcacatcag
       61 aagtgaacgt gatccgcacc ctttttgtcg ttcctgcagg ggatgcaaga tcatcgaaaa
      121 cccttgcacg gattgtgtga agcttaattc gaagcagatc aacgaactcg ccgaactggc
      181 tagaaagagt gagatacgaa aagaacaaga acgccgcaaa cgagctcgac agcggaacgt
      241 ttcagcttct caggtctcta ccccgagttc gacggcttcc acgccgggta aagtaaggaa
      301 aacggccaag aagcgttcgc caaaaacgaa gacgaagcac ccaaagggca tcacactgcc
      361 atcatcttct aaaaaatctt gtacacagat cccggaccta tccgacacga tgaccgggaa
      421 agacatcaac ccaacgaagg aaatcccatc caccttcctg gtgatttcgg aaacggatga
      481 caccttcgtc gaacaaaacg aaggtgaatc ttcaccttcg ctgcatgaac aacttctccc
      541 tacacagctt ggaatgcctt cggacaccag ggagatggcc gacgaacagg agggctcccc
      601 ctcatcgctc acactttcga ctggcctgga tcatgatcca cattacgact taacggacaa
      661 gataacccag attcttaagg atgttctacc aagatacttg ccacaaagaa catcggatca
      721 ggcttttcgg gatggatcca tccccggaat cgtcccggca agggccggta aaacaagcac
      781 cgacgccgtt acgaacctca ttcatgaaga agtgcttctg gcccgcacgg ccacactcgc
      841 cgcgggattg accctgccgc aacaccgccc acgacgggag gagatcggcc aaacagtcga
      901 tgacgcgccg gctagcccgg atccgccatc gtcgcaacaa tccacagacg tcccgtgcac
      961 accttcactt atacgtgaca acactcgcct tcgtccggcg tggaggattt acaaggcgcc
     1021 ggtatcgaaa cgtgcatatg aggctgacaa gccgtcaaga atgactagaa aattaaatcc
     1081 gtttgccgac ccacgaaaac gtctcctcac tgagacagat gaatcgataa gcaaaagaca
     1141 gagaatgaac gtcttcaaaa acgaatttgg tgcaacacta tatgatcatt cggacctacc
     1201 gtggccggat caaaccacaa acgtcaccgt ttcccaacca gtggttttcg tcgacaaccc
     1261 aaggtcacca tcccctgaga aggaagacca actaccttcg ggtaccaaac cacgaatgcc
     1321 accttcggcc aggagtttac c
//
LOCUS       DASTWE010001194         1727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1098659, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001194 DASTWE010000000
VERSION     DASTWE010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098659"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1727)
                     /locus_tag="VFT30_12435"
     CDS             complement(<1..>1727)
                     /locus_tag="VFT30_12435"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093489.1"
                     /translation="DLPEEEFLYGLRAYRNREYLRIGTRDLLGRATLEDTVQDLSHLA
                     EAAVQIAYEYVRSRLRVEYGEAVVEEDGRNRPLGFVVFGMGKFGGEELNFSSDIDLIY
                     LYERDGGPTVGGSKGKTEPRNFFTQLAQRLTRTLSDLVAGGRVFRVDLRLRPDGRNGP
                     VVNSLPNTLLYYESYGQTWERVALLKARPVAGDRALGEQFLHDISPFVFRRYLDFSTI
                     EDIKEMKARVERALPNRDHAALNVKLGRGGIREIEFVAQTLQLIHAGKDPHVRHRSTL
                     PALDRLVEGKYLSASDRDVLREAYQFLRHVEHKLQVVHDRQTHTLPSDEEGLRALARR
                     LRLEKDTEDRIQKVELESPSLKNSDAGRQPPDLLRAPDQPNDSGPRTSDPGLSRALNS
                     APRPIIPDLRMDEVVHFRSVLNRHTDAVHQIFHSLFHAPEEARKELDPEIPSLLQELH
                     QEERALWHLQQLGFHNLQESYEYLKLLHDGPPHAPASPKRRKLLYALAPKLFQEVSRA
                     ADPDRALAAMANLIASIGARSSFLSLLRENDATLRTLIGLFGTSEYLTQIFLLHPELL
                     DSLVRADLVR"
BASE COUNT          385 a          460 c          500 g          382 t
ORIGIN      
        1 cgaacgaggt cagcccggac gagactatca agtaactccg ggtgaaggag gaagatctgc
       61 gtcaaatatt cgcttgttcc aaagagaccg atcaaggtgc gcagcgttgc gtcattctct
      121 cggaggagcg agaggaaact agaccgcgcg ccaattgacg cgatcaaatt cgccatcgcc
      181 gccagcgcgc gatcgggatc ggcggcgcgg gacacttcct ggaatagttt gggcgcgagc
      241 gcgtacaaaa gcttgcgtcg tttcggggac gccggcgcat gcggcgggcc gtcgtgaaga
      301 agtttcagat attcgtaact ctcctggaga ttatgaaagc cgagctgctg cagatgccac
      361 agcgcccgct cttcctgatg caattcttgc aagagggacg gaatctccgg atcaagctcc
      421 ttccgtgctt cctcaggggc gtgaaacaag gagtgaaata tttgatggac ggcgtcggtg
      481 tgtctattca aaacggaacg gaagtgaacg acttcatcca ttcggaggtc agggattatg
      541 gggcgggggg cagaattcag agcccgagaa agcccggggt ccgaggtccg gggtccggaa
      601 tcattaggtt ggtccggagc ccgtaaaagg tccggcggct gacgtccggc gtccgagttt
      661 ttgagacttg gggattctaa ttctaccttc tgtattctgt cttctgtatc cttttcgagc
      721 cgtagtcgcc gcgccaaagc tcgcagccct tcctcgtcgc tcgggagtgt gtgcgtttgg
      781 cgatcatgga ctacctgcag cttatgctcg acatggcgga gaaactgata cgcctcgcgg
      841 aggacatcac ggtctgatgc cgatagatat ttcccctcga ctaaccggtc gagagcgggg
      901 agcgtactgc ggtgacgaac gtggggatct tttccggcat gaatgagctg gagagtctgc
      961 gcgacaaact cgatttcacg aattccgccg cggcccagtt tcacgttgag cgcggcatgg
     1021 tctctattgg gtagggcgcg ttcgacacga gccttcattt ctttaatgtc ttcaatcgta
     1081 gaaaagtcca ggtatcgacg aaacacaaac ggggagatgt cgtgcaagaa ttgttcgcct
     1141 aaagctctgt ccccggcaac cggacgtgct ttgaggagcg ccacacgttc ccaagtctgc
     1201 ccgtaggatt catagtagag caacgtgttg ggtaacgaat tcacgaccgg gccgttcctg
     1261 ccgtcgggac gaaggcgcaa atcgacacga aagacacgac ctccggcaac gagatcactt
     1321 aatgttcgtg ttaatcgttg ggcaagttgg gtgaagaaat tacgcggttc ggtctttcct
     1381 ttgctcccgc cgaccgtcgg gccaccatcg cgttcgtata aataaatgag atcaatatca
     1441 gaactgaagt tcagttcttc accgccgaat ttccccatgc cgaagacgac gaaaccgagc
     1501 ggacgattcc ggccgtcttc ttcgactacg gcctcaccgt attccacgcg gagacgagag
     1561 cgcacatact cgtacgcgat ctggacagct gcttcggcca aatgactcag atcttggacc
     1621 gtgtcctcta atgtcgccct tcctaaaagg tcccgtgtcc cgattcgcag atactcacgg
     1681 ttccgatacg ctcggagacc gtaaaggaat tcctcttcgg gaagatc
//
LOCUS       DASTWE010001195         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_1389564, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001195 DASTWE010000000
VERSION     DASTWE010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389564"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFT30_12440"
     CDS             complement(<1..254)
                     /locus_tag="VFT30_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5093490.1"
                     /translation="MVDMEARIGFVGVGRMGANMARRLNERGYRIAAVQDHHAEASKS
                     LAHDLNSEAAASPARVAEISDIVITVVSDDAAMRDIYAEQ"
     gene            complement(296..>1130)
                     /locus_tag="VFT30_12445"
     CDS             complement(296..>1130)
                     /locus_tag="VFT30_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093491.1"
                     /translation="AATRYGASSAATLPPSATASTLMIGRGPHTETLASPAPVALTQT
                     LPAPSDIQAGPQKAASFDPTSLVGTWHAHVAQGTTQHDLLLIIQPNLKWSLSSMMQFD
                     GILNAEAGLWSLERANTFKGLGWKGTYRKTTPTSFASTGSIQATWKRLTTDDSPTRIP
                     FELWKLRREATNVPVFQLKTVDPDLVGQWEGSGTYAGGSAAFVWAIKPTAATDLLIVD
                     TLRGTVQTKDGIPQLQPIPKKQQRVSVIAFHDRGFTTTDGKTKLRWTKLRSEPTEDRQ
                     L"
BASE COUNT          219 a          297 c          350 g          264 t
ORIGIN      
        1 tcctgttccg cataaatgtc ccgcatcgcg gcgtcgtccg acacgacggt gatgacgata
       61 tccgatattt cagcgacacg tgccggtgac gcggccgctt ccgagttcag gtcgtgcgcc
      121 agggattttg acgcttcggc atgatggtcc tggaccgctg cgatgcggta tccccgttcg
      181 ttcaatcgcc tggccatatt ggcgcccatc cgacctactc cgacgaaccc gatgcgagct
      241 tccatgtcta ccatgagagc atcatttccg gacatgcaat caaagatggg gatcgctaga
      301 gttgacggtc ctctgtcggt tcagaacgaa gtttggtcca tcgaagtttg gtcttcccat
      361 cggtcgtggt aaatcctcgg tcatggaaag cgatgacgct gacgcgttgt tgcttctttg
      421 gaataggttg gagctgggga atgccgtctt tggtttgcac agtcccgcga agcgtatcga
      481 caatcagcaa atcggtagca gcggtgggtt tgatcgccca tacgaacgca gcggaacctc
      541 cggcataggt gcctgatccc tcccattgcc cgactaagtc gggatcgacg gtcttgagtt
      601 ggaagaccgg tacgttggtt gcttcgcgtc ggagtttcca cagttcgaat ggaatgcgcg
      661 tgggggagtc atcggttgtg agtctcttcc aggtggcctg aatactgcca gtgctggcga
      721 acgaagtcgg tgttgtctta cggtaagtcc ccttccatcc aagacccttg aacgtgttcg
      781 ctcgctcgag tgaccagaga cctgcttcag cgttcaagat accgtcaaac tgcatcatcg
      841 atgagagcga ccacttcaga ttaggctgaa tgatgaggag caggtcatgt tgagtcgtac
      901 cctgtgcgac atgcgcgtgc cacgttccta ccaggctcgt cggatcaaac gatgcagctt
      961 tttggggccc agcctggata tcagaaggcg cagggagcgt ttgtgtgagc gccaccgggg
     1021 ctggcgaagc gagggtctca gtgtggggtc cacggccgat catgagggtt gaggctgtag
     1081 cggaaggagg gagagtcgct gccgaggacg ctccgtacct ggttgcggcg
//
LOCUS       DASTWE010001196         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_322775, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001196 DASTWE010000000
VERSION     DASTWE010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322775"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1024)
                     /locus_tag="VFT30_12450"
     CDS             complement(<1..>1024)
                     /locus_tag="VFT30_12450"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEU5093492.1"
                     /translation="GFLGLGPWYYDNGSTEVTRADERHDRVDVVTRGFLGLTVGCSRC
                     HDHKYDPIPTADYYGLAGVFANTIYEEYPRVPKSVLADFQKIEDQIDQKQKILGEIQQ
                     NQGAQLAKSLAFATANYLQGVWEVTGPQKKEMAAVVEARKLDFELLDRWIKYMEKPTD
                     KYKNKEPWQAMMKKGGKAEEAKKLAEAFQDQVVTVMLAKNELDEENKVIADKAMDGTK
                     RKKRTNKPSNFVTNEDFCPGCNLRLKNLSEQDTSFWTEIFQRMLNDNDDPIAMMAMGM
                     RNMKPGVLVFRGWGLESRIGSEAQMQINAIKKDIDDARKKLEPAYPYLHGVKDSDNPV
                     NLSIHLR"
BASE COUNT          162 a          286 c          293 g          283 t
ORIGIN      
        1 cccggagatg gatcgaaaga ttgacagggt tgtcggaatc cttcactccg tgtaggtaag
       61 gataggcggg ctccagtttc ttacgggcgt cgtcgatgtc cttcttgatg gcgttgatct
      121 gcatctgcgc ctcggagccg attcgcgatt ccagtcccca cccgcggaac accagaacgc
      181 cgggcttcat gtttcgcatt cccatcgcca tcatggcgat cggatcgtcg ttgtcgttca
      241 gcatgcgctg gaagatctct gtccagaagc tagtgtcttg ttcggagaga ttcttcaagc
      301 ggagattgca gccggggcag aagtcttcgt tggtgacgaa gttactgggc ttgttggtgc
      361 gtttttttct cttcgtgcca tccatcgcct tgtcggcgat gactttgttt tcctcgtcca
      421 gctcattctt ggcgagcatg acggtgacca cctgatcctg aaacgcttcg gccagcttct
      481 tggcttcctc ggccttgccg ccttttttca tcatggcctg ccacggctct ttgttcttgt
      541 acttgtccgt gggcttttcc atgtacttga tccagcggtc cagcagttcg aaatcgagtt
      601 tgcgggcttc gactaccgcg gccatctctt ttttctgcgg cccggtgact tcccacactc
      661 cttgcaggta attggcggtg gcgaaggcga ggcttttcgc aagctgggcg ccctggttct
      721 gctgaatttc gccgaggatc ttttgcttct gatcgatctg gtcttcgatc ttttggaaat
      781 cggccaggac cgatttcggg actcgcgggt actcctcgta gatcgtgttg gcgaagactc
      841 ccgccaagcc gtaatagtcg gctgtgggga tcggatcgta tttgtgatcg tggcagcgcg
      901 agcagccaac cgtcagtccc aggaatccgc gggtcaccac gtccacacgg tcgtggcgct
      961 catcggctcg agtgacttcc gttgagccgt tgtcgtagta ccagggcccg agtccgagga
     1021 atcc
//
LOCUS       DASTWE010001197         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_419908, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001197 DASTWE010000000
VERSION     DASTWE010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419908"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2264
                     /locus_tag="VFT30_12455"
     CDS             <1..>2264
                     /locus_tag="VFT30_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5093493.1"
                     /translation="PSSRLIDEEPYHILMEQVRFELGSIAAHCIRVFLEHGPTYFSGY
                     RSNEMMYRTNDIFNFVEDNRLILSQGVSLKQAHKLYTEWCEQTDTRNIYKQYQFRDLM
                     RDYFKEFHAEIMIDGVRHRSFFSELRELDKFSWKGLQPAPPKDWLTFDSDISILDEYL
                     KDMPAQEASSKGTPKRAWSEVKTTLSDIDTSVEHFVQVPEQLVVIDIDLKDKHGKKSL
                     EACLEEAAKWPPTYAEPSKSGEGIHLHYEFDGDVSRLRPVYSDGIEVKTLLGNASLRR
                     RFRGGNNLAITKINSGLPLKEEKVLAPKTMQTEKGLRELVLKGLRKEVHAYTKPSMDF
                     IKMVTDEAYNNGVVFDISDMWDDIMSFAMSSSNQRSICIETAMSIKLKSEEDVPADPD
                     EDKPEVIFDVEVFPNLNALGWMYDRDDAEVVTVVNPSPAEVEELISRTRLEGWNNRGY
                     DNHILWAISLGWPPERVYDLSRRIIAENDRRALFGSAYNLAYADLYDCASNANKKSLK
                     KWEIDLGLPHMELDLPWDQPVPENRVLDVLKYLENDVRSTRVVKHHIIEDLKARQILA
                     ELSGLEVCNTNRQHTERLIFGKLGPNDQPDLVYTDLREQFPGYEFNQFAPGKEKSTYK
                     GYKVSEGGWVKSKPGMYENVAVLDIASMHPTSIVELNMFGKDTKNFKRLLDIRLAIKK
                     GDYDTAIDLDERLAPYLTDKASAKALSDALKIVINSVYGLTASTFPHKFRDPNNIDNI
                     VAKRGALFMVDLVE"
BASE COUNT          568 a          608 c          614 g          474 t
ORIGIN      
        1 cccagcagtc gcctcatcga cgaagagcct taccacatcc tcatggagca ggtcaggttt
       61 gaactcggat cgattgctgc tcactgcatt cgtgtgttcc tcgagcacgg acctacatat
      121 ttctcgggtt accgttcgaa cgagatgatg taccgcacca acgacatctt caacttcgta
      181 gaggacaacc gtctcatctt gtcgcaggga gtcagcctaa agcaggctca caagctgtac
      241 accgagtggt gtgagcagac agacaccagg aacatctaca agcaatacca gttccgggat
      301 ctgatgcgcg actacttcaa ggagttccat gctgagatca tgatcgatgg cgtacgccac
      361 cggtctttct tcagcgaact gcgtgagctg gacaagttct cgtggaaggg tcttcaaccg
      421 gctcctccca aggactggct tacgttcgac tccgatattt caatactgga cgaatacctc
      481 aaggacatgc ctgcgcagga agccagcagt aagggcacac caaagcgggc gtggtctgaa
      541 gttaagacga cgcttagtga catcgacacc tcagttgagc acttcgtcca ggtcccagag
      601 caactcgtcg tcattgacat cgacttgaag gacaagcatg gtaagaaatc tttggaagcg
      661 tgcttggagg aggctgctaa gtggccccca acttacgcag agcccagcaa atctggcgaa
      721 ggtatccacc tgcattacga gttcgatggg gacgtcagtc gacttcgtcc cgtttacagt
      781 gacggaattg aggtcaagac gctgctgggt aacgcttcac ttaggcgacg gtttagaggt
      841 ggaaacaacc ttgccattac caagatcaac tccggactcc cactaaagga ggagaaggtg
      901 ctcgcaccca aaaccatgca gacggagaag gggttgcgag aactcgtcct caagggtctg
      961 cgcaaggagg tccacgccta cacgaagccg agcatggact tcatcaagat ggtcaccgac
     1021 gaggcctaca acaatggtgt agtcttcgat atttcggaca tgtgggatga catcatgtcg
     1081 ttcgcgatga gctcctccaa ccagcggagc atctgcatcg aaaccgcaat gtcgatcaag
     1141 ctgaagagcg aagaggatgt tcctgcggat cccgatgagg acaagccgga ggtcatcttc
     1201 gacgtcgagg tcttccccaa cctgaatgca ttgggttgga tgtacgaccg agacgacgct
     1261 gaagtagtca ccgttgtcaa tccgtcaccc gcagaggtgg aagagttgat ctcccggacg
     1321 cgcctggaag gctggaacaa ccggggatac gacaaccata ttctgtgggc aatttcgctg
     1381 ggttggccgc cggagagagt ctacgacttg tctcggcgca ttattgccga gaacgatcgt
     1441 agggcgctct tcgggagtgc ttacaacctc gcatacgcag atctctacga ctgcgcctca
     1501 aacgccaaca agaagtcact gaagaagtgg gagattgacc ttggcctacc gcatatggag
     1561 ctggatcttc cttgggatca gccagttcca gagaatcgtg tgctcgatgt cctcaagtac
     1621 ctcgaaaacg acgttcgatc tactcgagtg gtcaagcacc acatcatcga agacctcaaa
     1681 gcgcgacaga tcctcgcgga actgagcggg ctggaggtct gtaacaccaa ccgtcagcac
     1741 acggagcggt tgatatttgg aaagctcggt ccgaacgacc aaccagatct tgtgtacacg
     1801 gatctgaggg aacagttccc gggctacgag ttcaaccagt tcgctccagg caaagagaag
     1861 tccacataca agggctacaa ggtcagcgaa ggcggttggg tcaagtccaa gccaggtatg
     1921 tatgagaacg ttgcagttct cgacatcgcg tcaatgcatc ccacgtcgat cgtggagctc
     1981 aacatgttcg gcaaggacac caagaatttc aagcgtttgc tggatattcg tctggcgatc
     2041 aagaagggtg actacgacac agccatcgat ctcgatgagc ggttggcccc atatttgacc
     2101 gataaggcgt ctgcgaaggc cctctcggac gcactcaaga ttgtcatcaa ctccgtttac
     2161 ggtctcaccg cttccacctt cccgcacaag ttccgggatc ccaacaacat cgacaacatc
     2221 gtcgccaagc gtggtgcgtt gttcatggtg gacctggtgg agtt
//
LOCUS       DASTWE010001198         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate SMAG_U2378 k141_743229, whole
            genome shotgun sequence.
ACCESSION   DASTWE010001198 DASTWE010000000
VERSION     DASTWE010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960840
            Sequence Read Archive: SRR25158463
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (soil metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 02:24:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,492
            CDSs (total)                      :: 2,470
            Genes (coding)                    :: 2,462
            CDSs (with protein)               :: 2,462
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 3 of 8
            Pseudo Genes (multiple problems)  :: 2 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743229"
                     /isolate="SMAG_U2378"
                     /isolation_source="soil"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64388889 N 117.4927778 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VFT30_12460"
     CDS             complement(<1..994)
                     /locus_tag="VFT30_12460"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEU5093494.1"
                     /translation="MHRTFDPFRLLLISVAGWLGQQQRDAIEYLREENRVLREQLGDR
                     RLRLNDDQRRRLAAKARMLGRRILREITTIVTPETLLAWHRRLIAQKYDGSKNRGPGR
                     PRTRGEIEKLVVRMATENRDWGYRRIQGALANLGHEVARGTVANILKAHGMEPMPERS
                     RKTTWREFLSRHWELIVAADFFTVDVWTGSGLTRFLVLFLIDLSTRRVEIAGIAAKAD
                     GIWMTQLARNFCDDQDGFLTGKRYLIHDRDPLFTAEFQETLAASGVKSVKLPPRSPNL
                     NPHAERFVRTIKESCLNRMILLGEGSLRKTVREFMAHYHGERNHQGLGNRLISPD"
BASE COUNT          199 a          309 c          290 g          230 t
ORIGIN      
        1 catccgggga gatcaacctg ttgccaagac cctggtggtt gcgttcgcca tggtaatgcg
       61 ccataaattc gcgaaccgtc tttcgcagag agccttctcc gagtaggatc attcggttta
      121 agcaggattc tttgatggtt ctgacaaatc gctctgcatg gggattcaga ttgggcgacc
      181 gcggcgggag tttcacagac ttcacgccac tggcggccag cgtttcctga aactccgcag
      241 tgaacaaggg atcgcggtcg tgaatcaggt atcgctttcc cgtcaagaac ccgtcctgat
      301 catcacagaa gttgcgcgct agctgggtca tccagatccc gtccgccttg gctgcgatgc
      361 cagcgatctc aacgcgtcgc gtcgataggt caatcagaaa gagcaccaga aatctggtca
      421 ggcccgagcc cgtccacaca tcgacggtga agaagtctgc cgccacaatc agttcccagt
      481 gccggctcag aaactctctc catgtggtct ttctgctgcg ttccggcatg ggttccatgc
      541 catgcgcctt gagaatgttg gcgaccgtgc cgcgtgcgac ctcatggccc agattggcta
      601 acgctccctg gatcctccgg tagccccaat cgcggttctc cgttgccatg cgaacgacca
      661 gtttctcgat ctcgccccga gttcgggggc ggcccgggcc acggttcttg ctgccgtcgt
      721 acttctgtgc gatcagtctc cgatgccaag ccaggagagt ttccggcgta acgattgtcg
      781 tgatttctcg aagaatgcga cgcccaagca tcctcgcctt ggctgccaac cggcggcgct
      841 gatcgtcatt caagcggagt cgcctgtcgc cgagttgttc ccgcagtact cgattctcct
      901 cgcggaggta ttcgatcgcg tcacgctgtt gttggcccaa ccaaccggcg acagaaatca
      961 gcagcagtcg aaacgggtca aacgtccggt gcatccggat agagtactag cacggagttg
     1021 tgtgaggc
//
